LOCUS       DASUOM010000001         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1328221,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000001 DASUOM010000000
VERSION     DASUOM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328221"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFY27_00005"
     CDS             <1..1084
                     /locus_tag="VFY27_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5763923.1"
                     /translation="GSDYGLVEVDRWPIRSLSVICFIYRVPLAANRESVVERLQADPR
                     VESAQLVHSFTTQADPLTGYDDTYASLQRGLDVMEISAAHQYSRGQGVRIAIIDGDAD
                     VNHEDLEGRLTSTLLLADPDKSPNSTHGTAVASVIGANANNARGMVGIAPDSKMELYV
                     ACWAEQGAFGAVCDSFTLAKALDTLLGDPADILNMSLIGPYDPLLARLLKELERSGVI
                     IVAAQPSQERKASGFPASFGGVIGVDSSDGRPAASRNISTASSRPRGVYAPGEQIMVA
                     VPDDAYDFRSGTSLSAAHVSGVIALLLAVSPNIPREIVRNYLQESQHGSGSALPSVNA
                     CVVLQLADRSRECRPDPPPSIAPAMH"
     gene            complement(1081..1788)
                     /locus_tag="VFY27_00010"
     CDS             complement(1081..1788)
                     /locus_tag="VFY27_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX5763924.1"
                     /translation="MKKTRFLLSVIVLLGAALPMSPARAADVTGYGVLTSDYVFRGVS
                     YSDGHASVQAGADVALESGLYLGAWASSVDIGGGPARQRDWQLNYYLGYSQELGSDWS
                     IGVNAVLYTFPNTVGETDYDFLEYSIVANFDDRAWIEYSHCPDLFHSGTRTHNLDLYA
                     EWPLPAQLMLGGGAGYYDVSELTDSSYTYWQLGVTRAFGPIDIDLRYHGTSRAVPIIS
                     TPESAEERIALAARFVF"
     gene            2123..3034
                     /gene="gnd"
                     /locus_tag="VFY27_00015"
     CDS             2123..3034
                     /gene="gnd"
                     /locus_tag="VFY27_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5763925.1"
                     /translation="MEIGVVGAGKMGGNMARRWRAGGIDVAVFDRSAAALEEMAAVSG
                     MRTFPSLPALVEALPAPRLLWLMLPTGKPTEQTIEELLALLAPGDLIVDGANAHYRDS
                     QRHAKRLEERSVTFVDAGVSGGIWGINEGYGLMFGGEAAAIERVAPLARILAPSPDKG
                     WVHCGPAGAGHFAKMVHNGIEYGLMQAYAEGFSLLAARQHDLELPLQEIAEAWCHGTV
                     IRSWLLELTAGVLQDGETLASIAAEIADSGEGRWTAQEAIDLGVPAPVINAALMARFS
                     SQGSGDFGARLLAMLRNAFGGHAIPRN"
     gene            complement(3046..>3713)
                     /gene="pgl"
                     /locus_tag="VFY27_00020"
     CDS             complement(3046..>3713)
                     /gene="pgl"
                     /locus_tag="VFY27_00020"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5763926.1"
                     /translation="WRVSEDAAGAAAAFIAQCLRQAMDERGLATVALSGGRSPWGMFE
                     RLADERVEWDRVHVLQVDERIVPLDDKARNWSHFLEGSLAERIPQQNRHPIPVEVDAE
                     LAVGRYSATLIQWAGVPPQLDVVHLGIGEDGHTASLFTDDVLLQDRRHWVGVSRVYEG
                     HRRLTLTLPVLNCARRIAWFAIGAKRREVLTRLRKGDESIAASHVRRDNAVVFTDVAT
                     ADA"
BASE COUNT          743 a         1163 c         1104 g          703 t
ORIGIN      
        1 cggtagtgac tacggcctgg ttgaggtcga tcgctggccg atacgctcgc tgtcagtcat
       61 ctgtttcatc tacagggtgc ccctcgccgc gaatcgcgaa agtgtcgttg agcgcctgca
      121 ggccgatccg cgtgtcgaat cggcacaact ggtgcattcc ttcacgaccc aggcagatcc
      181 actcacgggg tatgacgaca cctacgcgag cctgcagcgc ggcctcgacg tcatggaaat
      241 ttccgctgca catcaatatt cacgcgggca aggcgtgcga atagcgatta tcgacggcga
      301 cgcggacgta aatcacgaag acctggaggg acgtctcacc agtacgctcc tgcttgctga
      361 tcccgataag tcgccgaata gcacgcacgg cacggcggta gcgagcgtca taggcgccaa
      421 tgccaacaat gccaggggaa tggtcggcat cgcccccgat tcgaaaatgg agttgtacgt
      481 cgcctgctgg gccgaacaag gcgcattcgg cgccgtatgt gacagcttca cgcttgcgaa
      541 agcgctggat acgctcctcg gagatccggc agacattctc aacatgagtt tgatcggccc
      601 ttacgatcca ttgcttgccc ggctgctgaa ggagctcgaa cgttcgggcg tcatcattgt
      661 ggcagcgcag ccgtcacagg agaggaaggc cagcgggttc cctgcaagct tcggcggagt
      721 gattggtgtc gacagcagcg acggacgtcc tgcggcaagc cggaacatca gcaccgcttc
      781 ctcgcggccg cgcggtgtct atgcacccgg agaacagatc atggttgcag taccggacga
      841 tgcgtacgac tttcgctccg gaacctcgtt gtccgctgcg catgtgagcg gcgtaatcgc
      901 cttgttgctt gcagtctccc cgaatatccc gcgcgagatc gtccggaatt acttgcagga
      961 atcgcagcat ggttcgggat ccgcattacc atccgtcaac gcctgcgtcg tactgcagct
     1021 ggccgaccga tcaagggagt gccggccaga cccgccacct tcgatcgcac cggcaatgca
     1081 ttagaaaacg aatcgcgcag cgagtgcgat acgttcctcc gcgctctccg gcgtgctgat
     1141 gatcggcacg gctcgactgg tgccgtgata gcgcagatcg atgtctatcg ggccaaacgc
     1201 gcgggtgacg ccaagttgcc aataggtgta gctgctatcg gtcagttccg aaacgtcgta
     1261 gtaacccgcc ccgcctccaa gcatcagctg cgccggcagc ggccattccg catacagatc
     1321 gaggttgtgc gtccgcgtcc cggaatgaaa caggtccgga cagtgcgagt attcgatcca
     1381 ggcgcgatcg tcgaaattcg cgacaatcga atactcgaga aagtcataat cggtctcgcc
     1441 caccgtattc gggaacgtgt aaaggaccgc attgacaccg atactccagt cacttcctaa
     1501 ctcctggcta taacccaggt aatagttcag ttgccaatcg cgctgccgtg caggaccacc
     1561 gccgatatcg acactggacg cccaggcacc gagatacagg ccggactcca gcgcgacgtc
     1621 cgcgcccgcc tggacggaag catggccatc gctgtaggac acgccgcgaa agacgtaatc
     1681 gcttgtcagg accccatagc cggtgacatc cgccgctcga gctggcgaca ttggtaacgc
     1741 agcgcccagc agtactatga cgctgaggag aaaacgggtc ttcttcatcg agtcacggta
     1801 gccatccccg agttggaaac gatcctgcgc accctccgcg gaaacatttc ctcgatttcg
     1861 gatgagatct gtaacgcaag ctattgacta tactacatcc tgaggcttgc aacttcgccc
     1921 ctgttcccct ctggttcgca acccgcgatg accggacagc cgatcaggat catgggcacg
     1981 agagctgcag cgttcgacca ccataccgca cgcggcgagc ggttattgcc tttgctgagc
     2041 tgacgacgta ttcatgccgg ataacttaca ggtttcgaca acggcaccgc atgctgccgt
     2101 gaccgcagca aggaacatca cgatggaaat tggcgttgtt ggcgcaggca agatgggcgg
     2161 aaacatggca cggcggtggc gagccggcgg cattgatgtc gcggtgtttg atcgttccgc
     2221 cgctgcgctc gaagaaatgg ctgccgtgtc cggaatgcgg acattcccga gtctgccggc
     2281 gctggtcgag gcgctgccgg ctcctcgcct gctctggctg atgctgccca ccggcaagcc
     2341 cacggaacag actatcgagg aattgctggc actgctggct ccaggcgatt tgatcgtcga
     2401 cggagccaat gcacattatc gcgactcgca aaggcacgcg aagcgtcttg aagaacggag
     2461 cgtgacattc gtcgatgcag gcgtgtccgg cggcatctgg gggatcaatg agggttatgg
     2521 cctgatgttc ggcggcgagg cggcggccat cgaacgcgtc gccccgctcg ctcgcatcct
     2581 cgccccgtca ccggacaagg gctgggtaca ctgcggccct gccggtgcgg gccatttcgc
     2641 gaagatggtg cacaacggga tcgagtacgg gctgatgcag gcatatgccg aaggtttctc
     2701 gctgctggcc gcgcgccagc acgacctcga acttcctctt caggagattg ctgaagcgtg
     2761 gtgccacggc acggttattc gctcctggct gctcgagctg acagccggcg ttttgcagga
     2821 tggagagacg ctggccagca tcgccgcgga aattgcggat tccggtgaag gacggtggac
     2881 cgcacaggaa gctatcgacc tgggagtacc cgcccctgtc atcaacgcag cactcatggc
     2941 gcgcttttcc tcgcagggca gcggtgattt cggtgctcgc ttgctcgcca tgctgcgcaa
     3001 cgccttcggt ggtcacgcca ttccccggaa ctagcaatgg aagagtcagg cgtcggccgt
     3061 ggcgacgtcg gtgaagacta ccgcattgtc acgccgcaca tggctcgcgg ctatgctctc
     3121 atctcccttc cgcaaacgcg tcaggacctc gcgtcgcttg gccccgatgg caaaccatgc
     3181 aatcctgcgc gcgcagttga gcaccggcag agtcagggtc agccggcgat ggccttcgta
     3241 gacccggctc acgcccaccc agtgccgccg atcctgaagg aggacatcgt cagtgaataa
     3301 tgaggcggta tggccgtcct ctccgatgcc caaatgcaca acgtccagtt gcggcggtac
     3361 gcccgcccac tgaatgagcg tggcggaata ccgcccgacc gcgagttcgg catctacttc
     3421 gaccggtatc ggatgccggt tctgctgcgg aatccgctct gcaagcgaac cctccagaaa
     3481 gtgagaccag ttccgcgcct tgtcatcgag gggcactatt cgctcatcca cctgcaacac
     3541 gtgcacgcga tcccactcca cgcgttcgtc ggccagccgc tcgaacatgc cccacggaga
     3601 gcgaccaccg ctcaacgcca ccgttgcaag cccgcgttcg tccatggctt gccgcagaca
     3661 ttgtgcaatg aaggcagccg cggcacccgc ggcatcttcg cttacccgcc att
//
LOCUS       DASUOM010000002         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1809623,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000002 DASUOM010000000
VERSION     DASUOM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809623"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFY27_00025"
     CDS             <1..1887
                     /locus_tag="VFY27_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5763927.1"
                     /translation="ERVEVLRGPQGTLFGRNTTGGLVHYVSKRPTEELDGYFNATLGD
                     FNQVKLEGALGGPITDAVAGRLSLATNQHDGWTENRSGPDYNETDARAARGQLLFTPS
                     DELDILLSGWWTDNDANVGAWQHQATGFNADGVSVPLAPGQQDMSVDCNADGVLDAGD
                     ARPAPGTDCFGYRDADGDPWAGEFDRDGRVEVETSGLVASVDWQIGNIGVTSITAFQT
                     VERLQSEDTEASPFPWILPTFAAETDTFTQELRFDGGAEDFRWLAGLFYFDNEVDGNY
                     ILDLTNLGFVFFDANYVQETDSLAVFGQVEYDLNDQWTLIAGARFANEEKTLDYLNVD
                     ESGLFEFLFGDNTAFDFDSASVGDLARHDNDSVSGKLELDWRPGDNLLLFGSISQGTK
                     SAGFNVGFLDQTFLFASNTPETIPFDEETLRSYEIGFKSTILDGRARLNGSAFYYDYE
                     DFQTFRFEILNQVIFNTDAEVSGAELEFQASPAEGWDIALGLSVLDATAEGIPDPAGG
                     PPRERTMVAAPEFSANALVRYAWPAFGGEVALQGWANYQGEMFYDIQNVPVSREDGYT
                     VGNLRASYTSGDQRWEVAAFAHNVTDEEYLSYTFDFTGTFGFNQQAYGPPRWFGVSLQ
                     YNME"
     gene            1953..3119
                     /locus_tag="VFY27_00030"
     CDS             1953..3119
                     /locus_tag="VFY27_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5763928.1"
                     /translation="MLEQVIDGDRALEANSFRGLSAAILMGVIGPEVFIVQPGFVQGL
                     VVYLGLTDSQAGYTASAEMFGIAATTVLLTFVAHKVNWRIVFTGSLVVMFITNALSTL
                     VHDFETFTALRFASGLGAGGLVSLSFAAIGLTENPDRNFGYLIMWVLIYGALGLMLMP
                     SAYALGGIDAVLWFFALFPLVGLPFVKYLPHTGENAVVVEQDAVTLPLGLRGLALLAM
                     LAYFLAQGVVWAYLFLIGLSGDLSEQQVANGLTMSQFAGIAGAFLAALVGNRYGRSLP
                     LTFGILGGAVCLWFLVGKMEFLVFATAVCVYNFAWNLTHPYLLAAMASFDRRGRVVVY
                     AVAMQMVGLAVGPGLAASVIDSGRYVNVNILGAALFILSLAFVLPPVIARARGQ"
     gene            3133..3945
                     /locus_tag="VFY27_00035"
     CDS             3133..3945
                     /locus_tag="VFY27_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5763929.1"
                     /translation="MFADLSGKVAIVTGAGQGIGRAIAEVFATAGARVMVATRTASHG
                     ETTVGRIEKAGGTARLCARDIGTEESVKSVVRETAEAWGGIDIMVHNAASFLGGPVEH
                     YSEGDLETVLSVNLKACFRFSAACIPEFRKRGGGRLLFTSSVTGPRVAMPGTSYYAAS
                     KGGINAFIRTAALELAREGITVNGVEPGFIRTAAMELLADAEGLERMTQYIPAGYMGA
                     PEDIAHAMLYLASDEARYITGQTICVDGGSTLPESPVFLDELEGIKELAGSS"
     gene            3986..>4551
                     /locus_tag="VFY27_00040"
     CDS             3986..>4551
                     /locus_tag="VFY27_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5763930.1"
                     /translation="MSRKYDMTINGETVRASEYREIRNPANRDEVVGLMPLGTTGHLE
                     QAIDAARSAFQRWRYSSDEERADACRTIAKVVGDNTDELKVLLSKEQGKPLKGLGAEF
                     ELGGCAAWASYTAGLSLPMKVLEASEKSRIEMHREPLGVVGSITPWNWPLLIAIWHVV
                     PAVRTGNAVVIKPSPFTPLGTLRMIELIS"
BASE COUNT          832 a         1397 c         1437 g          885 t
ORIGIN      
        1 gaaagggtcg aggtcctccg cggaccgcag ggcacgctgt tcggccgcaa tacgaccggc
       61 gggctcgtgc actacgttag caagcgcccg accgaggaac tcgacggata cttcaacgcc
      121 acgctcggcg acttcaacca ggtgaaactc gaaggtgctc tcggcgggcc gatcaccgac
      181 gccgtcgcgg gccggctgtc gctcgcgacc aaccagcacg atgggtggac ggagaatcgc
      241 agcggccctg actacaacga aacggatgcc agggcggcgc ggggccagct gctgttcaca
      301 ccgtcagacg agctcgacat cctgctgagc ggatggtgga cggacaacga cgcgaacgta
      361 ggcgcctggc agcatcaggc aacgggcttc aatgccgatg gggtgagcgt gccgctcgca
      421 ccgggccaac aggacatgtc tgtcgattgt aatgccgacg gagtcctgga cgcgggcgat
      481 gcccgcccgg cacccggcac cgactgcttc ggctaccgcg acgcggacgg cgatccctgg
      541 gccggggagt tcgaccgcga cgggcgtgtc gaggtggaaa ccagcggcct ggtggccagc
      601 gtcgactggc agatcggcaa catcggcgtc acctcgatta ccgctttcca gaccgttgag
      661 cgcctgcagt ccgaggacac cgaagcgagc ccgttcccgt ggattctgcc tacctttgcc
      721 gcagaaaccg acaccttcac gcaggaactg cgtttcgacg gaggcgccga agattttcgc
      781 tggctcgcgg ggctgttcta tttcgacaac gaggtggacg ggaactatat cctcgacctc
      841 accaacctcg ggttcgtgtt cttcgatgcg aattacgtcc aggaaacgga ttcgctcgca
      901 gttttcggcc aggtcgaata cgacctgaat gaccagtgga ccctgatcgc cggcgcccgc
      961 tttgcaaacg aagagaaaac gcttgattac ctgaacgtcg acgaatcagg attgttcgag
     1021 tttctgttcg gtgacaatac tgcgttcgac ttcgactcgg catcggtagg tgacctggcc
     1081 aggcacgaca acgactccgt cagcggcaag cttgaactcg actggcggcc gggcgacaac
     1141 ctgctgctct tcggctccat ctcccagggc acgaagtccg caggtttcaa cgtcggtttc
     1201 ctcgaccaga ccttcctgtt cgccagcaat acgccggaaa ccatcccctt cgacgaggag
     1261 acgctccgca gttacgagat tggtttcaag tcaacgatcc tggacggccg tgcccgcttg
     1321 aacggcagcg cgttctatta cgattacgag gacttccaga ccttccgttt cgagattctg
     1381 aaccaggtga tcttcaatac cgatgccgaa gtcagcggtg cggagctgga gttccaggcc
     1441 tcgccggcgg aaggctggga catcgcgctg ggactgtcgg ttctcgacgc caccgctgaa
     1501 ggcattcccg acccggccgg cggcccgccg cgcgagcgga ccatggtcgc ggcgccggaa
     1561 ttttcggcga acgcgctggt gcgttacgca tggccggcgt ttggcggtga agtggcgctg
     1621 cagggctggg ccaattacca gggcgagatg ttttacgaca tccagaacgt gcccgtatcg
     1681 cgggaggatg gctacaccgt tgggaacctc cgggcctcat acacgtcggg tgaccagcgc
     1741 tgggaggttg ccgccttcgc acacaacgtc accgacgagg aatacctgag ttacacgttc
     1801 gacttcaccg gcaccttcgg ttttaaccag caggcttatg gaccaccgcg ttggttcggc
     1861 gtgagcttgc aatacaacat ggagtgacca gccgccgcag atgtagcccg gctgtttggc
     1921 catagacctg ggtgccgttc gtaggagtag ttttgctgga gcaggtcatc gacggggaca
     1981 gggccctgga ggcgaattcc ttccggggac tgtcggccgc catcctcatg ggcgtcatcg
     2041 gcccggaggt gttcatagtt cagccgggct tcgtgcaggg cctggtggtt tatctcggcc
     2101 ttaccgacag ccaggccggt tacacggcgt ccgccgagat gttcggcatc gcggcaacca
     2161 ctgtcctgct gacgtttgtc gcgcacaagg tcaattggcg tatcgtcttc accggttcgc
     2221 tcgtcgtgat gttcatcact aatgccctgt cgaccctcgt acacgatttt gaaactttca
     2281 cagcgttgcg attcgcatcc ggtctcgggg cgggtggact ggtgtcgctg agctttgcgg
     2341 cgatcgggct cacggagaat ccggatcgga atttcggcta cctgatcatg tgggtgctga
     2401 tctacggcgc cctcgggctc atgttgatgc cgagcgcata cgctcttgga ggcatcgatg
     2461 cggtgctgtg gttcttcgcg ctgtttccgc tggtcggact cccgttcgtc aaataccttc
     2521 cccacaccgg cgaaaacgcg gtggtcgtgg aacaggatgc ggtgacgctg ccgcttggcc
     2581 tgcgcggcct cgccttgctc gccatgctcg cctattttct cgcgcagggc gtggtctggg
     2641 cctacctgtt cctgatcggg ctttccggtg acttgagcga acagcaggtg gcgaatggcc
     2701 tgacgatgtc ccagttcgcc ggcatcgcag gggctttcct ggccgccctc gtcggcaacc
     2761 gctacggccg ttcactgccg ctcaccttcg ggatcctggg tggcgctgtg tgcctctggt
     2821 tcctcgtcgg caaaatggaa ttcctcgtgt ttgcaactgc cgtgtgcgtt tacaattttg
     2881 cctggaacct gacccatcct tacctgctgg cggcgatggc aagcttcgat cgccggggcc
     2941 gcgtggtggt gtacgcggtg gccatgcaga tggtggggct cgctgtcgga cccggccttg
     3001 ccgcctccgt gatcgatagc ggccgctacg taaacgtcaa catactcggg gccgcccttt
     3061 tcattttgag ccttgctttc gtgctgccgc cggtgattgc ccgtgcgcgc ggccagtaac
     3121 aggagaacag ccttgtttgc cgatctcagc ggaaaagtag ccatcgtcac cggtgcggga
     3181 cagggcatcg gccgcgccat cgcggaagtc tttgccacag cgggcgcccg ggtgatggtg
     3241 gcaacccgca ccgccagtca cggcgaaacc accgtggggc ggatcgaaaa agccggcggc
     3301 actgcgcgcc tgtgcgcgcg agacatcggc acggaagaaa gcgtcaagag cgtcgtacgt
     3361 gaaacggcgg aggcctgggg tggcatcgac ataatggtgc acaatgcggc ctcgttcctg
     3421 ggcggtcctg tcgagcatta ttccgaaggc gacctggaga cggtgctttc ggtcaacctc
     3481 aaagcttgct tccgcttctc tgccgcgtgc ataccggaat tccgcaaacg cggcggcggc
     3541 cgcctgctgt ttacctcgtc agtgaccggc ccccgcgttg ccatgcccgg cacctcctat
     3601 tacgccgcct cgaagggcgg catcaacgcg ttcattcgca cggcggccct cgaactcgcc
     3661 cgcgaaggca taacagtcaa tggagtcgaa cccgggttta tccgcaccgc cgccatggaa
     3721 ctgttagccg atgcggaagg cctcgagcgg atgacgcagt acattccggc tggctacatg
     3781 ggtgcgccgg aagatattgc tcacgcgatg ctttacctcg ccagcgacga ggcgcgctac
     3841 atcactggcc agacgatttg tgtcgacggc gggtcaacgc tgccggagag cccggtattc
     3901 ctggatgaac tcgaggggat caaggaactg gccggctctt cataagcctt acaaccctgc
     3961 ccccaccaac cactgagaga agacgatgag cagaaaatac gatatgacga tcaacggcga
     4021 aaccgtgcgg gccagcgagt atcgcgagat ccgcaatccc gccaaccgcg atgaggtcgt
     4081 cgggctgatg ccgctcggaa ccaccggaca ccttgaacag gcgatcgacg cggcgcggag
     4141 tgctttccag cgctggcgct attcgagcga tgaggaacgc gcagacgcct gccgcaccat
     4201 tgccaaagtc gtcggcgaca acaccgacga actgaaagtc ctgctgagca aggaacaggg
     4261 gaagccgctc aagggcctgg gcgcggaatt cgagctgggc ggttgcgcgg catgggcttc
     4321 ctataccgcg ggtttgagcc tgccgatgaa ggtgctggag gcaagcgaga aaagcaggat
     4381 cgagatgcac cgcgagccgc ttggcgtcgt cggctcgatc acgccgtgga actggccgtt
     4441 gctgatcgcc atctggcatg tggtgccggc cgttcgcacc ggcaacgctg tcgtcatcaa
     4501 gccgtcgccg tttacgccgc tcggcacctt acgcatgatc gaactgattt c
//
LOCUS       DASUOM010000003         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_482144,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000003 DASUOM010000000
VERSION     DASUOM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482144"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFY27_00045"
     CDS             <1..815
                     /locus_tag="VFY27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HEX5763931.1"
                     /translation="PKLAVFKFCCCDGCQLQVLSLDDELLELGELFDIAYFNEASSRR
                     DPGPYDVALIDGSIAVPEDVSRIAWVRAQSRVLITIGACATAGGIQALRNWGDVEGWK
                     RSVYPRPEQVAALSTSTPISEHVHVDVELWGCPIDKQQLLHVLKDVHAGVQPRLPAHS
                     VCLECKRQGHVCVLVAQGQPCMGPVTRTGCGAICPGMQRDCYSCFGPVEPGKTGPGLP
                     PNTPSLARHFHRELGLIPVDVVRRFRGINGYTAPFQKESNVWERGDRPDDPR"
     gene            775..>2040
                     /locus_tag="VFY27_00050"
     CDS             775..>2040
                     /locus_tag="VFY27_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX5763932.1"
                     /translation="MSGSEETGRTIRVDRIARVEGEGALRVTVKDGRVDEVRLSIFEA
                     PRFFERFLEGRHISELPDLVARICGICPVAYQMSAVHAVEQIFGVTMEGPLRDLRRLL
                     YCGEWIESHALHVFLLHAPDFFGYESGLEMAKDYPDLVRRGLELRKTGNAILALLGGR
                     AIHPVSVRVGGFSRVPTRRELAGLKDMLSRTRDAAAETVRWVAGFDFPAFAQAYTFVA
                     LRSATEYPMNHGQVASTDGFEIPVAEFERHFVEHQVDHSHALQCSMDGRSYMVGPLAR
                     LHLNYDRLSAVTQDVLADARISLPLRNPYQAVIARAAEMLHAADEALRIIDTYEPPPA
                     PSLAFTMQSGTGTAATEAPRGLLYHRYAIDEDGTIRTVVIVPPTSQNQRRMEDDLRAL
                     LPGVLSLDNESAARACERVIRCYDPCISCA"
BASE COUNT          345 a          646 c          664 g          385 t
ORIGIN      
        1 gtcccaagct tgccgtcttc aagttctgct gctgcgacgg ctgccagctc caagtcttga
       61 gcctcgacga cgaactgctg gaactcggcg aactgttcga catcgcgtat ttcaatgaag
      121 cgtcgagccg gcgcgatccg ggtccgtatg acgtcgccct gatcgacgga tcgatcgcgg
      181 tgccggagga cgtgtcgcgc atcgcctggg tccgcgccca atcccgggtg ctcatcacga
      241 tcggcgcctg tgccactgcc ggcggaatcc aagcgcttcg caattgggga gacgtggaag
      301 ggtggaagcg gagcgtgtat ccccgtcctg agcaggtcgc cgctctcagc acgtcgacac
      361 cgatctccga gcacgtgcac gtggacgtcg aattgtgggg ctgcccgatc gacaagcagc
      421 agctccttca cgtgctcaag gacgtgcatg ccggcgtgca gccgcgcctt cccgcacaca
      481 gcgtctgcct ggagtgcaaa cgccagggac acgtgtgcgt cctcgtggcg caaggccagc
      541 cctgcatggg ccctgtgaca cgaaccggct gcggcgcgat ctgccccggc atgcagcgcg
      601 actgttacag ctgcttcggg ccggtcgagc ccggaaagac cggtcccgga ctgccgccga
      661 acacgccgtc gctggctcgc cattttcatc gcgagttggg cttgattccc gtcgatgtgg
      721 ttcgccgttt tcgaggcatc aatgggtaca ccgccccctt tcagaaggag agcaatgtct
      781 gggagcgagg agaccggccg gacgatccgc gttgaccgga tcgcacgggt ggaaggcgaa
      841 ggagctttgc gcgtcaccgt gaaagacggc cgcgttgacg aggtacgcct gagcatcttc
      901 gaagccccgc gcttcttcga acgctttctc gaggggcggc acatctcgga gctgcccgat
      961 ttggtcgcga ggatctgcgg aatttgtccg gtggcctacc aaatgagcgc cgtgcacgcc
     1021 gtcgagcaga tcttcggcgt caccatggag ggtcccttgc gggacttgcg ccggctgctc
     1081 tactgcgggg aatggatcga aagccatgcc ttgcatgtgt ttctgctcca tgccccggac
     1141 ttcttcggct acgagagcgg cctcgagatg gccaaagact atccggatct ggtgcggcgc
     1201 ggactggagc tgcgcaagac gggcaatgcg attctcgccc tgctgggagg gcgcgcgatc
     1261 catccggtct ccgtgagggt aggcgggttt tcacgggtgc cgacgcgccg ggagttggcg
     1321 gggttgaaag acatgctgtc acgcacgcgc gatgccgcag ccgagacggt tcgctgggtg
     1381 gccgggttcg actttccggc atttgcacaa gcctatacgt tcgtcgcgct gcggtcggcc
     1441 acggaatacc ccatgaatca cgggcaagtg gcttcgacgg atggattcga gattccggtc
     1501 gccgagtttg aacggcattt tgtcgaacac caggtcgacc acagtcatgc gctccagtgc
     1561 tcgatggacg gccgctccta tatggtcggc ccgctggccc ggctccatct taattacgac
     1621 cggctgtcgg cggtgaccca ggacgtcctc gcggacgccc gcatcagcct gccgctcaga
     1681 aatccctacc aggcggtgat cgcgcgcgcg gccgagatgt tacatgccgc cgacgaggcc
     1741 cttcgtatca tcgacaccta cgagccaccg ccggcgccgt ccctggcgtt cacgatgcag
     1801 agcggcaccg gcacggctgc gaccgaagcg ccccgcgggc tgctgtacca tcgctatgcg
     1861 atcgacgagg acggaacgat tcgcacggtc gtgatcgtgc cgcccacttc ccagaatcag
     1921 cggagaatgg aagatgatct gcgggcgttg cttcccggtg tgttgagcct ggacaatgag
     1981 tctgcggccc gtgcatgcga acgggtcatc cgctgctacg atccctgcat ctcctgcgcg
//
LOCUS       DASUOM010000004         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_965772,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000004 DASUOM010000000
VERSION     DASUOM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965772"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1162
                     /locus_tag="VFY27_00055"
     CDS             53..1162
                     /locus_tag="VFY27_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763933.1"
                     /translation="MITLERRKFGWVSLALVLVAGFASDQVLAEPYLAVYKGMHCSGC
                     HSHPAGGGKRNAYGNVFAQSEMPAARLGAADADLWSGEIAKWLSVGADLRAAYEYVDV
                     PDGDESSEFSISRGTAYVQATLIPERLSLYLDQQIAPGSGINREAYVRLNSESGKFHL
                     AAGQFFLPYGLRLQDDTAFVRQVTGVNFSNPDRGVQLGYEAGPWSTQLTVTNGSGGGN
                     ETDTGKQMSWIAHFVRQRWRAGASFNFNDADAGDRQMQNVFFGLRTGPIAWLAEADLI
                     TDDLPSGAEQDAITGLLEGNWLFRKGHNLKVSYEYFDPDDNVDEDHQVRWSLVWEYTP
                     MQFLQGRIGVRLYDGVPQVDLQNRDVYFAEIHGFF"
     gene            complement(1712..2359)
                     /locus_tag="VFY27_00060"
     CDS             complement(1712..2359)
                     /locus_tag="VFY27_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5763934.1"
                     /translation="MRKTKIEPIRDPLKDRLLTPQNCAVVLIDYQPEQVATVKSSPIE
                     EVMLNVKAVCKLARAYEVPVVLSTVGVDMGVNQGTIPEMRDELPDVEEIDRTGVNSWE
                     DADFRAAVEVTGRKKIVMCGLWTEVCLAFPTLDMLAEGYEVYPVIDAVGGISKVSHES
                     AIQRMIQAGAHPITAISFGSELMRNWARRDSDNLRKVLRWYFSARRDLAGHDDNG"
BASE COUNT          490 a          737 c          736 g          520 t
ORIGIN      
        1 cattcgccag tggattgcag atggtgcagc gcgttaaccg cggcgctacg gaatgattac
       61 cctggaacgc cgaaaattcg gctgggtgtc gctggcgctg gtactggtcg caggcttcgc
      121 gagcgatcag gtcctggcag agccctatct cgcagtctac aagggcatgc actgttccgg
      181 ttgccacagc catccggccg gcggcggaaa acgcaacgcg tatggaaacg ttttcgcgca
      241 gtccgaaatg ccggccgcgc gcctgggcgc cgcagatgcc gatctctggt cgggtgaaat
      301 tgcgaagtgg ctgtcggtcg gcgccgacct gcgcgcggct tatgagtacg ttgacgtacc
      361 ggatggggat gagtcgtcgg agttcagcat cagccgcggt acggcctacg tgcaggcgac
      421 actgataccg gagcgtctct cgctgtacct ggatcagcag attgcgccag gctccggcat
      481 caaccgcgag gcttacgtgc gtctcaacag cgaaagcggg aagtttcatc tcgcagcagg
      541 gcaattcttc ctgccatacg gcctgcggct gcaggacgac accgcgtttg tccggcaggt
      601 caccggagtc aacttctcca atccggaccg cggcgtgcaa ctcggatacg aagcgggccc
      661 atggtcgact cagctgacgg tcacgaacgg cagcggtggc ggcaatgaaa cggacaccgg
      721 caaacaaatg agctggatcg cacacttcgt tcggcagcga tggcgcgcgg gcgcgagttt
      781 caacttcaac gacgccgacg ccggcgacag gcagatgcaa aacgtattct tcggcctgcg
      841 caccggaccg atcgcctggc tcgccgaggc ggatctcatc accgacgatc tcccatcagg
      901 tgcggagcag gacgctatca ccggtttgct tgaaggaaac tggctctttc gtaaaggcca
      961 taacctgaag gtcagctacg agtatttcga tcccgacgat aacgtggacg aagaccacca
     1021 ggtacggtgg agcctggtgt gggagtacac gccgatgcag ttcctgcaag gcaggatagg
     1081 ggtgcgtctt tacgacgggg ttccgcaagt cgacctgcag aaccgcgacg tgtactttgc
     1141 ggagatacat ggctttttct gaggacgatc aaccaattgg tgcttggcct gttcgccatc
     1201 ccttgaatca gtgtgactct ggctgtcccg gacgcccatc ccttgggaaa acggctttgt
     1261 gtgtatttgc tgccaagatc tgccgcacgg ttatttcctg ctcgtcgcgc ttccgttctg
     1321 gctacaatct gtgtttgacg cgcacggaac caggcggacc gtacgcggac gcaaactcta
     1381 tcgaaaatat ttggtaatgg atccagacaa aaacgtcagc gtacttttcg tgtgcatggg
     1441 catcatattt tgattcctgt aataacaatc tcttagatga ttcgggcaca atcctggcta
     1501 caggttccca agcgtaatct ggatcgccgg acggtgggcg tttacggacc gattcggaac
     1561 tcttcggaag tttggcagta gggaatcgca cttgcgaacg taacgtacgc gcagagtcag
     1621 cccgttgccg ggtgcctgac aaccgcacta agtatgcggc agattctggc aataggagtc
     1681 gtggcaagaa gtcctctcgc cctaggatgt atcagccgtt atcgtcatgc cccgcgagat
     1741 cgcgcctggc tgaaaagtac cagcgcagca ccttgcgcaa gttgtcggaa tcccgccgcg
     1801 cccagttgcg catcagttcg gagccgaagg agatcgcggt gatcggatgc gcgccggcct
     1861 ggatcatgcg ctgaatcgcg ctctcgtgcg acaccttcga gatgccgccg acggcatcga
     1921 tcaccgggta aacttcataa ccttcggcca gcatgtcgag cgtcgggaat gcgaggcaga
     1981 cctccgtcca caacccgcac atcacgatct tcttgcgccc cgtcacctcg accgcggccc
     2041 gaaagtcggc gtcctcccat gagttgacgc cggtgcggtc gatctcctca acgtccggca
     2101 gctcgtcgcg catctccggg atcgtgccct ggttcactcc catgtccact cccacggtcg
     2161 agagcaccac cggcacctca taggcgcgag ccagcttgca gactgctttc acattgagca
     2221 tcacctcctc gatcggtgat gacttgaccg tggcaacctg ctcgggctgg tagtcgatca
     2281 gcacgaccgc gcagttttgc ggcgtcagga gacgatcctt caacgggtcg cggatcggct
     2341 cgattttggt cttgcgcatg tcggattcct catctatttt cagtttgcgc cgcgtcgcgc
     2401 gacgagttgt ccgtgctcat tcggccgaag cgaccggcgt tgaaatcgtt gatcgccctg
     2461 cgaatctcgg cctcactgtt cat
//
LOCUS       DASUOM010000005         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_481862,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000005 DASUOM010000000
VERSION     DASUOM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481862"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFY27_00065"
     CDS             complement(<1..153)
                     /locus_tag="VFY27_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5763935.1"
                     /translation="MPELPEVETSRLGIAPWLEQQQVRKVIVRERRLRWPVPETIDTV
                     LPGKFIR"
     gene            356..1537
                     /locus_tag="VFY27_00070"
     CDS             356..1537
                     /locus_tag="VFY27_00070"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5763936.1"
                     /translation="MEYLYGVLNLSFWGYVIVTVLMVQITMMAVTLYLHRDQAHRAIN
                     LHPVLRHFFRFWIWCTSGMLTREWVAVHRKHHAYCETPDDPHSPQIYGLKKVLLEGAE
                     LYRVEKDRPETLEKFGRGAPDDWLEKNIYLRYPWGGILLTFAIDLVLFGVPGITIFAV
                     QMISMPLFAAGVINGLGHHSGYRNFECDDAATNLLPWGVLVGGEELHNNHHAFPTSAK
                     FSVRSWEFDIGWLYICVFRALNLAKVNKVAPRPMLKPSPRQSVDIENLRAIIVNRMHV
                     LRDYTKQVTLPVLRRERAAAAGDAAWRKAKKLLVRQPKLLDERARHSLSQLLSDNATL
                     QTVYEFREKLRTLWSGANVSNERLLQQLKDWCAEAEASGISVLEDFAARLRSYHLASS
                     R"
     gene            complement(1717..1872)
                     /gene="rpmG"
                     /locus_tag="VFY27_00075"
     CDS             complement(1717..1872)
                     /gene="rpmG"
                     /locus_tag="VFY27_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5763937.1"
                     /translation="MREKIRLVSSAGTGHFYTTTKNKRLHPDKMETKKYDPRARKHVV
                     YKEAKIK"
     gene            complement(1884..2120)
                     /gene="rpmB"
                     /locus_tag="VFY27_00080"
     CDS             complement(1884..2120)
                     /gene="rpmB"
                     /locus_tag="VFY27_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5763938.1"
                     /translation="MSRVCQVTGRRPQSGNNVSHANNKTRRRFLPNLQNKRFWLESEK
                     RFVRLRVSHKGLRIIDKHGLEKVLADLRAQGQKV"
     gene            complement(2275..2949)
                     /gene="radC"
                     /locus_tag="VFY27_00085"
     CDS             complement(2275..2949)
                     /gene="radC"
                     /locus_tag="VFY27_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019571520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5763939.1"
                     /translation="MVTDFPGKAKLPRAVPPPAGAGSLSEAELLAVLVRQGSSHEAAL
                     ELSIRLLTRFGGLRPLLVATQSELAAEEGVGNSRLAVLRAIPELARRYYEQALPTGHA
                     IRSPADTEAFLMARLRDLPHELFCCIYLDNRHRIIAFEELFRGTIDGTSVYPREVVKQ
                     ALSVNAAAVILAHNHPSGVAEPSQADERITRRLKAALELVDIRLLDHLVIGDGISTSL
                     ASRGMI"
     gene            3114..>4076
                     /gene="coaBC"
                     /locus_tag="VFY27_00090"
     CDS             3114..>4076
                     /gene="coaBC"
                     /locus_tag="VFY27_00090"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5763940.1"
                     /translation="MQKLNILLGVTGSVAAYKAPELVRKLRDGGATVQVVMTEAASRF
                     ITATTLQAVSARPVRDNLWDEEAEAAMSHIELARWADVVLIAPASAEIISRLASGSAG
                     DLLTTICLATEAPLTIAPAMNRVMWAHPAVQANREVLAGRGVTILGPGRGGQACGEFG
                     EGRMLEPREIAEAVLGAERKAPRRAEPGQEPPPQPLLGRTVMITAGPTREAIDPVRFI
                     SNHSSGKMGYALAEAAQTAGARVILISGPVSLPPPANVERVCVETAEQMFEETHNRID
                     GVDIFIGAAAVSDYRPANTRDQKIKKSNAGLRIDLVKAPDTLASI"
BASE COUNT          812 a         1263 c         1250 g          751 t
ORIGIN      
        1 ccgaatgaat tttcccggca gaacggtgtc tatggtttca ggtacgggcc accgcagccg
       61 ccgttccctg acaatgacct tgcggacctg ttgctgttcc agccacggag cgataccgag
      121 acggctggtt tctacctccg gaagttcagg catttctgtc cattaatgtg accggcgccc
      181 actatacagt ggcggggaaa gcgcgagaat gactgacctt ttcccgacgc gtcctggcgg
      241 cgctggtata gtgacgctcg cgcattgcct tcctgtaaac ctccttaacg ccaggcggac
      301 gtccgccgcc ccgccgagtg cgggcagatg caagttgacg attggaaact gacgaatgga
      361 atacctttac ggagtgctga acctgtcctt ctggggctac gtcatcgtga cggtcctgat
      421 ggtgcagatt acgatgatgg cggtcacctt gtacctgcac cgcgaccagg cgcatcgcgc
      481 catcaatctg catccggtct taaggcactt cttccgattc tggatctggt gtacctccgg
      541 aatgctgacc agggaatggg ttgccgttca ccgcaaacat cacgcttact gcgagacccc
      601 ggacgacccc cacagtccac aaatatacgg cctgaaaaaa gtactgctcg agggtgccga
      661 gctttatcgt gtcgagaaag accggccgga aactctggag aaattcggcc gcggcgcacc
      721 tgacgactgg ctggagaaga acatttacct gcgctacccg tggggcggga tattgctgac
      781 ctttgccatc gacctcgtgc tgttcggcgt cccgggcatc actatcttcg cagtacagat
      841 gatttcaatg ccgttgttcg cggccggcgt aatcaacggc cttgggcatc acagcggcta
      901 ccggaacttc gaatgcgacg acgcagcgac gaacctgctg ccctggggcg tgctggtcgg
      961 cggcgaggaa cttcacaaca atcaccatgc gttcccgact tccgccaagt tttcggtccg
     1021 cagctgggag ttcgacatcg gctggcttta catctgcgta ttccgtgcgc tgaacctggc
     1081 gaaggtgaac aaggtggcgc cccgcccgat gctcaagccg agccctcgac agagcgtcga
     1141 tatcgaaaac ctgcgtgcga tcatcgtgaa tcgcatgcac gtgctccgcg actatacgaa
     1201 acaggtgacg ctgcctgtgc tccgccgcga gcgggccgcc gctgccgggg atgcggcatg
     1261 gcggaaggcg aagaagttac tggtgcgcca gccgaagctt ctcgacgaac gcgcccggca
     1321 tagcctgagc cagctgctct cggataacgc caccctgcag accgtctatg aattccgcga
     1381 gaagttgcgg accctctgga gcggggcaaa cgtgagcaac gagcgactgc tgcaacagct
     1441 gaaagactgg tgcgcggaag cggaagcgag cgggatcagc gtcctcgagg atttcgcggc
     1501 acgcctgagg tcctaccacc tcgccagcag ccgctagcat tggtatctgt tgcccgtagg
     1561 acgggctcct gcagccagct atccgaagcc ggcgggaaca gaatcgctgt aagagcccgc
     1621 ctcgccggca acccctcacg tcagaactca gcatcgcacg accaaaaaaa accgccttcc
     1681 ggcggttttt tcgatgcgaa acctgaaagc agattgctac ttgatcttcg cttccttata
     1741 aaccacgtgc tttcttgctc gcggatcgta tttcttcgtc tccatcttgt ccgggtgcag
     1801 ccgcttgttc ttggtggtgg tatagaagtg gccggtgccg gcgctggaga caagcctgat
     1861 tttctcacgc attttggctt ctcctagacc ttctgtcctt gagcacgcag atccgcaagc
     1921 accttttcaa ggccgtgctt gtcgattatg cgaagcccct tgtgggacac gcgaagacgc
     1981 acgaaacgct tctccgactc gagccagaat cgcttgttct gcaggttggg cagaaaacga
     2041 cggcgcgtct tgttgttggc atgggaaacg ttgttgccgc tttgcggacg ccttcccgtg
     2101 acctggcaaa ccctggacat ggcggttaag tctctgattc agtagctggt taattcaaag
     2161 ttgcccgcgg aggcgggcaa agagcgggac tttataccag ccccgccggg gcgtttcaag
     2221 gccggtggcg gaggtccttg tcgaggggca cgatcataca tctttatatc tgcctcagat
     2281 catgccgcgg ctcgcaagcg aggtgctgat gccgtcaccg atgaccaggt ggtccagtag
     2341 ccggatgtcg acgagttcca gagccgcctt gagacggcgg gtgatgcgct cgtcagcctg
     2401 gctcggttcc gccacgcccg agggatgatt gtgtgcaagg atgaccgccg cggcattcac
     2461 cgacagcgcc tgcttcacca cctcccgcgg atagacgctg gtgccgtcga tggtgccacg
     2521 aaaaagctcc tcgaacgcga tgatgcggtg gcgattgtcg aggtagatgc agcagaacaa
     2581 ctcgtgcggc aggtcgcgca ggcgcgccat caggaaagcc tcggtgtccg cgggactccg
     2641 gatcgcgtgc ccggtgggca acgcctgctc gtaataccgc cgcgccagct caggtatagc
     2701 ccggagcacc gcgaggcgtg aattccccac accttcctcg gccgcgagct cgctctgcgt
     2761 cgcgaccagc agcgggcgca gcccgccaaa gcgcgtgagc agccgaattg aaagttcaag
     2821 agcagcttca tgactggaac cctgccgaac cagcaccgcc agcagttcag cctccgacag
     2881 ggagccggcc ccggctggag gcggcaccgc gcgcggcagc ttcgccttgc ccggaaagtc
     2941 ggtaaccacg gacgctcctt gtccagtacg atcgcagaga ccgtggacat cattgatcga
     3001 taccgccacc acggacaact gccaaatccc cgcaccgacg tcagattagg gaggttctgt
     3061 attcccgacg cacaccgggc taagctgtgc cgggttccat acagcgagta cccatgcaga
     3121 aactgaacat cctgctaggc gtcaccggca gcgtcgccgc ctacaaggct cccgagctcg
     3181 tccgcaagct tcgcgacggc ggcgcgacgg tgcaggtggt gatgacggag gcggcatcac
     3241 ggttcatcac ggcaaccact ctgcaggccg tgtcggcaag gccggtgcgc gacaacctgt
     3301 gggacgagga agccgaggcg gccatgagcc atatcgagct ggcccgctgg gcggacgtgg
     3361 tcctgatcgc gccggcttcc gccgaaatca tatcgagact cgcgtccggc tcggcgggtg
     3421 acctgttgac gaccatttgc ctggcgacag aagcaccgtt gaccattgcg ccggcgatga
     3481 accgcgtgat gtgggcgcac ccggcagtgc aggcgaacag ggaagtcctc gccggccgcg
     3541 gcgtcacgat cctcggaccg ggccgcggcg gacaggcctg cggggagttc ggtgagggac
     3601 gcatgctgga gccccgcgaa atcgcggaag ccgtgctggg cgcagaaagg aaggcgccga
     3661 ggcgagcgga gcccggccaa gagccgccgc cgcagcccct cctcggccgt acggtgatga
     3721 tcaccgcagg gcccacgcgc gaggctatcg acccggtgcg cttcatcagc aatcacagct
     3781 cgggaaaaat gggctacgcg cttgccgagg ccgcccaaac ggccggcgcc agggtgatcc
     3841 tgatcagcgg gcctgtgagc ctgccgcccc ctgccaacgt agaacgagtg tgtgtcgaaa
     3901 ccgccgaaca gatgttcgag gagactcaca accggatcga tggcgtcgac atattcatcg
     3961 gcgctgcggc cgtttcggat tatcggccgg ccaacacgcg ggaccagaag ataaagaaga
     4021 gcaatgcagg actcaggatc gacctcgtca aagcgcccga cacacttgcg tccatc
//
LOCUS       DASUOM010000006         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_965236,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000006 DASUOM010000000
VERSION     DASUOM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965236"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VFY27_00095"
     CDS             complement(<1..1770)
                     /locus_tag="VFY27_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5763941.1"
                     /translation="MNTAPPYAELHALSNFTFLRGASHPEELVHKAAELRYEALAITD
                     ECSVSGVVRAYMAAKDYGLKKFLIGSEFRLSQSLKLVVLVQNRQGYARLCRLITEGRR
                     AAKKGSYRLEASAFADGLPGCLLLWVPDNRLLLDEDDRWILDAFRERLWIAVELLADG
                     LERERIERLRVLGGKLELPLVASGDVHMHCRERRVLQDTVTAIRLGTTLDRAGFALYP
                     NGERYLRSRSRLASIYPEEFRQQALNVAGMIDFSLDELRYEYPDELVPEGETASGYLR
                     KLTGEGMRRRWPDGAPPKVVKLVEHELELIGSLEYEPYFLTVHDIVAYARSQGILCQG
                     RGSAANSAVCFCLGITEVDPARMAMLVERFISKERNEPPDIDVDFEHERREEVIQYIY
                     RKYGRERAALSATVITYQPRSALRDVGKALGLSGLQVDRLARSMQWWDGREVDTSRVI
                     EAGLDPESPVIRRLLYLVRQLMRFPRHLSQHVGGFVISRGPLSELVPVENATMKDRTV
                     IQWEKDDLDDLGLLKIDVLGLCMLTAIRRSFDLVRQFDGREYSLATVPAEDPVVYDMI
                     CEGDTMGVFQIESRAQMAMLPRLK"
     gene            complement(1972..3423)
                     /locus_tag="VFY27_00100"
     CDS             complement(1972..3423)
                     /locus_tag="VFY27_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5763942.1"
                     /translation="MNARDGAVTEAVFENQQGNRRILMADARAVELGITPGQSLNAAL
                     ALVPALQLEERNAEKESRVLERMAGWAERFTSIVSIEPPDLLLLEIRGSLQLFGDIPS
                     LRQQVEDGLISRGFRPSIAVAPTVLGSVWLARAGTKASIFDRRNLAGTLSPLPLHCLA
                     WPASVREALHGMGIYCIGDCLRLPRQGFARRFGAARLLQLDRALGRLPDPRRSYRAPQ
                     RFCEEWELPEEQSDSGLLLQACRQLLSRLERFLTTRQIEVRHVQFSFYHLQSAATHLT
                     LGCRQAGRKSGQWFELLAIRFERLALPAPVIAIRLRSGAAQALQADEDSLFDTNRRQQ
                     RTSIDHLKERLSARIGDDCVHGITTVAEHRPQYAWSTAKDHRLPPCAAVPTHPQTCQS
                     PLLLADMRRTNSLLLRRPLWILPEPLPLTTNPEYHGPLKLVDGPERIETGWWDGKGIA
                     RDYYVATNAKGVHLWIYRNRNRQATWHLHGIFG"
     gene            complement(3625..4326)
                     /locus_tag="VFY27_00105"
     CDS             complement(3625..4326)
                     /locus_tag="VFY27_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763943.1"
                     /translation="MNDTLENLLQNPRVWRGSQRLGNPAAAFTACSTGYARLDDHLPG
                     GGWPRHALTEILLEHYGTGELKLLMPALARLSNADDNIGNGGNVNDHCADPAFEEAGW
                     IAWIAPPFQPYPPALLQWGINLQHMLMVHPRRDVEALWAAEQALSSGNCAAVLVWSDK
                     LDNRASRRLQLAAEKGQSLCFAFRPLEAAALSSAAALRLALHAGESGTDVRILKSRGG
                     RPVTVSGVIHHDYAV"
BASE COUNT          881 a         1396 c         1353 g          927 t
ORIGIN      
        1 cttcaatcgc ggcagcatgg ccatctgtgc gcgtgactcg atctggaaca cgcccatggt
       61 gtcgccctcg cagatcatgt cgtacaccac gggatcttcg gcgggcacgg tggcaagcga
      121 gtattcgcgg ccgtcgaact gccggaccag gtcgaagctg cgccgtatgg ccgtcagcat
      181 gcaaaggccc agcacatcga tcttcaacag gccgaggtca tcgagatcgt ccttctccca
      241 ctggattacc gttcgatcct tcatcgtggc gttttcgacc ggcaccagtt ccgacaatgg
      301 cccgcgggag attacgaagc cgccgacatg ctgcgacagg tgtcgcggga aacgcatcag
      361 ctggcgcacg aggtagagca gccgccggat taccggactc tccggatcga gaccggcttc
      421 gatcaccctg ctggtgtcca cttcgcggcc gtcccaccat tgcatcgaac gtgcaagccg
      481 gtcgacctgc aggccggaaa gtcccagcgc cttgccaacg tcgcgaagcg cgctgcgcgg
      541 ctggtaggtg attactgtcg cgctaagcgc cgcacgctcc cggccgtact tacggtaaat
      601 gtactggatg acttcctcgc ggcgctcgtg ctcgaagtcg acgtcgatat cgggtggttc
      661 gttacgctct ttcgaaatga aacgctccac cagcatcgcc atcctggcag ggtccacttc
      721 tgttatccct aagcagaagc acacggctga attcgccgcc gagccccggc cctggcaaag
      781 aatgccctgg ctgcgggcat aggcaacgat gtcgtggacg gtgaggaagt acggctcgta
      841 ttcgagactg ccgatcaact cgagctcgtg ttcgacgagc ttcactacct tcggcggtgc
      901 tccgtccggc catcttctcc gcatgccctc cccggtcagc ttgcgtaagt aaccagacgc
      961 tgtctcgccc tccggcacca gttcgtccgg atactcatat cgcaactcgt ccagtgaaaa
     1021 gtcgatcatc ccggcaacgt tcaatgcctg ttggcggaat tcttcgggat aaatacttgc
     1081 caggcggctc cttgaacgaa gataccgttc gccgttcgga tacagcgcaa aacctgcccg
     1141 gtcgagcgtg gtgcccaacc gtatcgccgt taccgtatcc tgcagcaccc gtcgttcacg
     1201 gcagtgcata tgcacgtcgc cgctcgcgac cagcggcagc tcgagcttac ctccgagaac
     1261 tcgcagccgc tcgatacgct cccgctcgag accgtccgcc agcagctcaa ccgcaatcca
     1321 caggcgttcg cgaaacgcat cgagtatcca gcgatcgtct tcatcgagca gcagcctgtt
     1381 gtccgggacc cagagcagga gacaaccggg tagtccatcg gcaaatgcgg aggcctcgag
     1441 ccggtagctt cccttcttcg ccgctcgcct gccctcggta atgagccggc acaggcgtgc
     1501 atagccttgc cggttctgca ccagcacgac gagcttcagg ctttgcgaaa gccggaactc
     1561 ggagccgatg aggaacttct tgagtccata gtcctttgcc gccatgtacg ctcttacgac
     1621 gccggacacc gagcattcgt cggtaatggc gagtgcctcg tacctgagtt cggcagcctt
     1681 gtgcacgagt tcttcggggt gggatgcgcc ccttaagaag gtgaaattgc tcaaggcgtg
     1741 gagttctgca taaggtggtg ctgtgttcat gcttttttaa gtcgccgcgt aattccgttc
     1801 atggataatg gcctttgcgt tttggcatcg ccggcggggt tgcctttagc ggaatggctc
     1861 ccttcggtcc gctttatttc cgctaaaggc aaccccaccg acgacgcctc ctcgtctgcg
     1921 taccctccgc ttgacctgac caggtgccat tcgttgaagt caatcgctgc atcaaccgaa
     1981 aatgccgtgc aggtgccagg tggcttgcct gttgcggttc cggtagatcc agaggtgcac
     2041 acctttggcg ttggttgcca cgtagtagtc cctggcgatg cccttgccgt cccaccagcc
     2101 ggtttcaatg cgctcgggac cgtcaaccag tttcagggga ccgtggtatt cgggattggt
     2161 ggtgagcggc agcggttccg ggagtatcca gagagggcgt cgcagcaaca ggctgttggt
     2221 tcgccgcatg tcggccagca ggagcggtga ctggcaagtt tgtgggtgag tcggcacggc
     2281 tgcacaggga ggcaatcgat gatctttagc cgtgctccag gcatattgtg gacgatgttc
     2341 ggctacggtc gtaatgccgt gaacgcaatc atcgccgatg cgcgcggaca atcgttcctt
     2401 caggtggtcg atggatgtac gttgctgccg gcggtttgtg tcgaacaggc tgtcttcatc
     2461 tgcctgcagt gcctgggcag cgccgctcct caaacgtatt gcaatgacgg gcgcaggcaa
     2521 tgccaggcgc tcgaagcgga tggcaagcag ttcaaaccac tgccccgatt tacgaccggc
     2581 ctgcctgcag ccgagtgtga gatgagttgc cgcgctctgc aggtggtaaa aactgaactg
     2641 cacatgccgg acctcgattt gccgcgtcgt taaaaaacgt tcgagacgtg acagcagctg
     2701 ccggcaggct tgaagcagca gtccactgtc gctctgctcc tcgggcagtt cccactcttc
     2761 gcaaaaacgt tgtggcgcac gatagcttct gcggggatcc ggcagacggc ctagagcccg
     2821 gtcgagttgc aacaacctgg cggcgccgaa tcggcgcgcg aaaccctgcc tcggcagccg
     2881 caggcaatcg ccgatgcaat aaatgcccat gccgtgcaac gcttcgcgga cagacgccgg
     2941 ccacgcgaga caatgcaagg gtagcgggct caaggttcct gccaggtttc tcctgtcgaa
     3001 aatggaagct ttcgtccccg cccttgccag ccagacagac ccgaggactg ttggtgcaac
     3061 ggcaatcgaa ggccggaaac cccgcgatat caatccatct tccacctgtt ggcgcaatga
     3121 cgggatgtct ccgaagagct gcaggcttcc gcggatttcc aggagcagca aatccggcgg
     3181 ttctatgctg acaatcgagg taaaacgttc ggcccagccc gccattcgtt ccagcacgcg
     3241 gctttccttt tccgcattac gctcttcgag ctgcaaggca ggaaccagcg ccagggcagc
     3301 gttcaacgac tggccgggtg tgatgccgag ttccactgcc cgggcatccg ccatcagtat
     3361 ccggcggtta ccctgctggt tctcgaaaac tgcttctgtc accgctccat cacgcgcgtt
     3421 caatgcttcc agcggcagtg agggaaagtg aatgcaaagc catagcggcg taactcggct
     3481 gcggcttgca tagacgggtg ggtcttttcg ccacggcaat tccggcggca gcacaagagc
     3541 aagcgattgc tgtgcggcgg gctgtgttac agggggagcc tggccatccc gtggtacggg
     3601 cagtttatcc gggggcggca tgggctacac cgcataatcg tgatgtatta cgccggagac
     3661 ggtgacaggc cggccaccac ggctcttgag aatacgcacg tccgtgccgc tttcacctgc
     3721 gtgtaaagca agacgcagtg cggcggcgga agaaagcgct gctgcctcaa gcggacgaaa
     3781 cgcaaaacac aagctttgcc ctttttcggc agcaagctgc aaacggcggc tcgccctgtt
     3841 gtccagttta tccgaccaca caaggacagc cgcgcaattg cctgaagaaa gcgcctgttc
     3901 ggcagcccat aatgcctcga catcccggcg aggatgcacc atcagcatgt gctggagatt
     3961 gattccccac tgcaacaggg ccggcggata aggctgaaac ggcggtgcga tccaggctat
     4021 ccatcctgct tcctcgaaag cagggtcggc gcaatgatcg tttacattac ctccgtttcc
     4081 aatattgtcg tctgcgttgc tcagccgcgc gagcgccggc atcagcagct tcagttcgcc
     4141 cgtcccgtaa tgttccagca ggatttcggt caacgcatgt cgcggccagc cgccgcccgg
     4201 gagatggtca tcgagccttg cgtacccggt cgaacacgcg gtgaacgcgg ctgcaggatt
     4261 gcccagccgc tggcttcccc gccagacccg cgggttctgc agcaaattct cgagcgtgtc
     4321 gttcattgaa agtggcctgt aacaggggaa gcggaagtgg gatcgcgaag ggacgattgg
     4381 ggctggcacc gtggtggctg gcgccggtct ggagcgaagc cgatagccgt agctatcggc
     4441 tgagtgaaga ccaagccaga cgccgccaga ccgagcgaga cagttccgat ttcgctgtta
     4501 caggccacta atggctcttc ctgccggttg cgttcggcat cggcactacg ccattct
//
LOCUS       DASUOM010000007         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1691088,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000007 DASUOM010000000
VERSION     DASUOM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691088"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /gene="tkt"
                     /locus_tag="VFY27_00110"
     CDS             complement(<1..2019)
                     /gene="tkt"
                     /locus_tag="VFY27_00110"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5763944.1"
                     /translation="MSSRTSRSTVVRRPDRRDLANAIRVLSMDAVQAANSGHPGMPMG
                     MADIAEVLWNDYLRHNPANPRWYDRDRFVLSNGHGSMLLYSLLHLTGYPLEMDELRNF
                     RQLGSKTPGHPEREQDAGIETTTGPLGQGLSNAVGMALCEKMLAAAFNRPGFDIVNHR
                     TWVFLGDGCLMEGISHEVCSLAGTLRLGKLVCFYDDNGISIDGKVTGWFSDNTAMRFE
                     AYGWHVVRDVDGHDSEAVSKAIEEALAEGGRPSMIICKTVIGYGAPNKQGSESTHGAA
                     LGEDEVAAARAELGWDSPPFVIPGEISDAWNAVDRGREREREWSDRFAEYRAKFPELA
                     GEFERRMKGELPASWAEGADAAIDDIAAAGKDMATRKASLQALNVFAPLLPELAGGSA
                     DLTGSNLTRHQHSVTVTGEDAAGNYIFFGVREFGMSGVCNGMALHKGIIPYCGTFLTL
                     SDYARNALRMAALMKLRNIFVYTHDSIGLGEDGPTHQPVEHVASLRIMPNMRVWRPCD
                     AVETAVAWRDAIERAGGPTSLVLTRQGVPHQQRTEDQLAAIRKGGYVLRDFGKSPQII
                     FIATGSEVGLAVGAASELENEGIAARVVSMPCTDLFDAQPEDWRQSVLPPEVGARIVI
                     EAGVGDFWWRYAGPSGRIIAMHGFGESAPAEELFQHFGFTVDNVLSAAR"
     gene            2355..>2891
                     /locus_tag="VFY27_00115"
     CDS             2355..>2891
                     /locus_tag="VFY27_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5763945.1"
                     /translation="MSHSYLFTSESVSEGHPDKIADQISDAVLDAILEQDTEARVACE
                     TLVKTGMVLVAGEVTTTAWVDIEALVRETVIGIGYSDSAMGFDGASCAVITALGKQSP
                     HIRQGVDRGDPENQGAGDQGLMFGYACNETSVLMPAPITYAHRLVRRQAEVRKAGTLP
                     WLRPDAKSQVTFVYEDDKP"
BASE COUNT          539 a          911 c          882 g          559 t
ORIGIN      
        1 cctggcggcg gaaagaacat tatcgaccgt aaacccgaaa tgttggaaca gctcctcggc
       61 tggcgcggac tcgccgaatc cgtgcatcgc gataattcgg cccgatggcc cggcatagcg
      121 ccaccagaaa tcgccgacac ctgcttcgat gacgatacgt gccccgacct cgggaggcag
      181 taccgactgt cgccagtctt ccggctgtgc atcgaacagg tcggtgcatg gcattgacac
      241 gacccttgcc gctatacctt cgttttcaag ttccgacgcg gcacccacgg ccaggccgac
      301 ttccgagccg gtcgcaataa aaatgatttg cggcgattta ccgaaatccc gcaggacgta
      361 gccgcctttg cggatcgcag ccagctgatc ttcggtcctc tgctggtgcg ggactccctg
      421 tcttgtcagc accaggctgg tcggtccgcc tgcgcgctcg atcgcatctc tccaggcgac
      481 ggcagtctcg acggcgtcgc acggccgcca gacccgcatg ttcggcatga tcctcaacga
      541 ggctacgtgc tcgaccggct ggtgcgtagg cccgtcctcg ccaagcccga tggaatcatg
      601 ggtgtatacg aatatgttgc gcagcttcat cagcgccgcc atgcgcagcg cgttccgcgc
      661 gtagtccgac aacgtcagga acgtgccgca gtagggaatg atgcccttgt gcagcgccat
      721 gccgttgcag acgcccgaca tgccgaattc ccgtacgccg aaaaagatgt agttgcctgc
      781 agcatcctca cccgtcacgg tgacggagtg ctggtgcctg gtcaggttgg aaccggtaag
      841 gtcggcggaa ccgcccgcga gctccggcaa cagcggagcg aacacgttca gtgcctggag
      901 cgaggctttg cgcgtcgcca tgtccttgcc cgcagccgcg atatcatcaa tcgccgcgtc
      961 cgcaccttca gcccacgagg ccggcagctc tcctttcatg cggcgctcga attctcccgc
     1021 gagttcggga aatttcgccc ggtactcggc gaagcgatcc gaccattcgc gttcccgctc
     1081 gcggccgcgg tccactgcgt tccacgcgtc ggaaatttcg cccgggatga cgaacggcgg
     1141 cgagtcccat ccaagttctg ctcgagctgc cgcaacctcg tcctcaccaa gggccgcgcc
     1201 atgggtgctt tcgctgccct gcttgttggg cgcaccatag ccgatcaccg tcttgcagat
     1261 gatcatcgac ggccgtccac cttccgccag tgcctcctcg attgctttcg acaccgcctc
     1321 gctgtcgtgc ccgtcgacgt cgcgcacgac atgccagcca tacgcctcga agcgcatggc
     1381 cgtattgtcg ctgaaccagc cggtcacctt gccgtcgatg gaaatgccgt tgtcgtcgta
     1441 gaagcagacg agcttgccca gcctgagcgt gccggcgagt gagcagactt cgtgcgatat
     1501 gccttccatc aggcagccgt caccgaggaa tacccaggtc cggtgattga cgatgtcgaa
     1561 gcccggacga ttgaacgccg ccgccagcat cttttcgcaa agcgccatgc caacggcgtt
     1621 ggacaagccc tgccccagcg gtccggtggt ggtttcgata ccggcatcct gctcgcgctc
     1681 aggatgcccc ggcgtcttcg aacccagttg ccggaaattg cggagctcgt ccatctccag
     1741 ggggtagccc gtcaggtgca acaagctgta aaggagcatc gatccgtgac cgttcgacag
     1801 cacgaagcga tcgcggtcgt accagcgggg attcgccgga ttatgtcgca ggtaatcgtt
     1861 ccacagcacc tcggcgatat ctgccatgcc catcggcatt cccgggtggc cggagtttgc
     1921 ggcctgcacg gcgtccatgc tgagcacgcg aattgcattg gcgagatctc tgcgatctgg
     1981 ccggcgaacg actgtcgagc ggctggttcg ggatgacata cttaccagaa ctccgattgt
     2041 ttcggggtcg cgtcacttga cttaaggaac agcccgaagg ctgggaatgg gcatgcagcg
     2101 taggtcgcag ccggtgcagg cgctttacat tttccctgtt tagtcgggtc gccgtcaatt
     2161 ttcgacgcaa aaaggcgcca gggatctctg cggtagaaat ttccgtcgcg aaaatcgacc
     2221 gctgtggtgc atttgcacga ctgcagggat gcaggagtcg gggcgaagca ggagccagag
     2281 ccgagtggaa ctatttctgc cgcgcagagc cctggggtac aatgcgggcc ggtttttgaa
     2341 gcagcgacgg ttaaatgagc cattcttacc tgttcacgtc ggagtcggta tccgagggtc
     2401 atcctgacaa gattgcagac cagatttcag atgcggtgct cgacgcgatc ctcgagcagg
     2461 ataccgaagc gagagttgcc tgcgaaacct tggtgaagac cggaatggtg ctggtcgccg
     2521 gcgaggtgac gaccactgcg tgggtcgaca tcgaggcact ggtgcgggag acggtgatcg
     2581 gcatcggcta ctcggactcc gcgatgggct tcgacggcgc ttcctgtgcc gtcatcacgg
     2641 cactcggcaa gcaatcgccg cacattcgcc agggcgtgga ccgtggcgac ccggagaacc
     2701 agggcgccgg cgaccagggt ttgatgttcg gttacgcctg caacgagacc agtgtcctga
     2761 tgcctgcacc cattacctac gctcaccgcc tcgtcaggcg ccaggccgag gtgcgcaagg
     2821 cgggaacgct cccgtggctg cggcccgacg cgaaaagcca ggttactttc gtctacgagg
     2881 atgacaagcc g
//
LOCUS       DASUOM010000008         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1085048,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000008 DASUOM010000000
VERSION     DASUOM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085048"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFY27_00120"
     CDS             <1..961
                     /locus_tag="VFY27_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5763946.1"
                     /translation="EGLELPTLDDVRFAARQLAPHIIRTPLLRLGFRDTSNPIYLKLE
                     NLQPIGAFKVRPMGNVMLSTGMDVLRKGAYTASSGNAGLGLAWIARQLGIEATVYAPD
                     TGPSAKLEAVRELGARVCRMSPDQWWEVIENGRHPTESGYYVDAVRNRSAMAGNGTIG
                     LEIVEQLPDVDTVIVPFGGGGLVCGIASILGALKPDTDIVVAESAAAAPLTAAIREGR
                     PVQVPMQPSFISGAGAPCVLNEMWPLIRRLVARTAVVPVTDVAEAVRLLFLHNRVVAE
                     GAGAISVAAALRRNAEAGTTVCVVSGGNIDREVFCNILNKQPT"
     gene            complement(1049..1219)
                     /locus_tag="VFY27_00125"
     CDS             complement(1049..1219)
                     /locus_tag="VFY27_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763947.1"
                     /translation="MTIISRTIGTCLFSAALIAGPNPAAAEVTVIHAGRLLTVAGEAP
                     ARRQSIVEGQNN"
     gene            complement(1242..2651)
                     /locus_tag="VFY27_00130"
     CDS             complement(1242..2651)
                     /locus_tag="VFY27_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5763948.1"
                     /translation="MRKPAPSKTSKNLKAALDALQSLLGEKFSSGMAVRDHHSHDESW
                     HSAKRPDAVCFAESTEDVAGALAICHRYRIPVVPFGTGTGLEGGTVAHQGGLCIDTSR
                     MQAIIEVNADDMDCRVQAGVTRKQLDRYLHDSGLFFPIDPGADASLGGMAATRASGTN
                     AVRYGTMRENVLSLKAVMADGSIVTTGRRSRKSAAGYDLTRLFVGSEGTLGIITELTL
                     RLHGRPEAISSGRVYFDDVDEAVNTAIQTIQCGIPIARVELLDAAQMEAINRYSGTCY
                     PGKDALFLEFHGSPASVKEQAETVGTICAALGGSDFEWTAFEEERRAMWDARHSAAYA
                     AMAVRPAAKVYATDVCVPISRLADCIRETRADVHKSTGISSFLLGHIGDGNFHYVFLV
                     DPDDPAEYKTVLDLSERMVQRAIDMGGTCTGEHGIGIGKQKFLNLEFGEAAVALMRTI
                     KAAVDPHDIMNPGKLLPPQ"
     gene            2718..3992
                     /locus_tag="VFY27_00135"
     CDS             2718..3992
                     /locus_tag="VFY27_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX5763949.1"
                     /translation="MPKHPGGMFKAIGPGVLYAATAVGASHLVQSTRAGASYGCALVL
                     LIAVTFVAKYPAFRFSAQYASATGHSLLHGYRYQGTWAVATYIIIAIGTMFAAVPAVT
                     LVTAGLAKVAFGLQMGTFGISVIILAGCTVVLIVGGYHVLDRLVKVLMVLLSVATIVA
                     TALVIPNVDWEVSGTIIPTGLVRTDVFFIVALLGLMPSSVDISVWHSLWSVAKARDTE
                     HPPTLNQSLADFHLGYVGAFVLALCFVLLGAGVMHNSGLQFESGATEFAAQLIELYKK
                     TLGEWSGPLIGIVAVAVMFSTVLTGLDGYPRAAVNLLHIFAEEKLPSDASQADKINRK
                     VYAAALILLVLGSVLILYFFVTSQRLVVDIAATIMFLFAPIIAWLNHRAMTSPEIMTA
                     ARPGRGLLVLSHLGVFWMAAFSVYYLYLRLSG"
     gene            complement(4006..>4526)
                     /locus_tag="VFY27_00140"
     CDS             complement(4006..>4526)
                     /locus_tag="VFY27_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5763950.1"
                     /translation="MVTTLTIGDNYSRLAEHPEFLDEPLYRATLTADEIETLKTDRRK
                     EYQERKWTWWMQIMTTIAQENLLKISEAGGILAAGTDQTTGPALHRELELLAGAGIAP
                     LEIIRIATLNGAIFLGKERELGSIEEGKIADLVLLGADPSRDINNAREIVQVIKDGQV
                     IDRSRLNLPINQ"
BASE COUNT          827 a         1371 c         1313 g         1015 t
ORIGIN      
        1 cgaaggcctc gagcttccca ctcttgatga cgttcggttc gcggcgcgcc aattggcccc
       61 gcacatcatc cgaacccccc tgcttcgcct tggttttcgc gacacatcga acccgatcta
      121 cctcaaactc gaaaatttgc agcccatcgg cgctttcaaa gtcaggccga tgggcaacgt
      181 aatgctgtcc actggtatgg atgttctgcg caaaggggct tacacagcca gttccgggaa
      241 tgccggtctc ggactggcct ggattgcgcg gcagctcggc atcgaagcca cggtatacgc
      301 gcccgacacc ggaccttccg ccaagctcga agcagttcgt gagcttggcg cgcgcgtctg
      361 ccgcatgagc cccgaccaat ggtgggaggt gattgaaaat ggcagacatc cgacagaatc
      421 cggctactac gtcgatgctg tcagaaatcg ctcggcgatg gcgggaaacg gtacgatcgg
      481 tcttgaaatc gtcgaacagc ttccggacgt cgatacggtc atcgttccct tcggcggcgg
      541 tggtctcgtc tgcggtatcg catcgatcct cggtgccctg aaacccgaca cggacatcgt
      601 cgtcgccgaa agcgcagcgg cggcgccgct caccgccgct atccgggaag ggcgcccggt
      661 gcaagttccg atgcagccat ccttcatctc cggggccggt gcgccgtgtg tactaaacga
      721 aatgtggccg cttataagac gcctcgttgc caggaccgcg gtggtaccgg tcacggacgt
      781 tgccgaagcg gtacggctcc tgttcttgca taaccgggtt gttgcggagg gggccggagc
      841 tatctcggtt gcggctgcgt tgcgcagaaa tgctgaagca ggcacgactg tttgcgtcgt
      901 atccggcggc aacatcgacc gggaagtttt ctgcaacatt ctgaacaagc agccaacgta
      961 agttgggcct gacggccact ggattgttga agcacatcat atgccaatgc atatattatc
     1021 tcaatcacca tcgcccgtac ccaggacttc aattattctg tccttccacg atgctttgcc
     1081 gtcttgcagg cgcttcaccg gcgaccgtca gcagccggcc cgcatgtatc acggtaactt
     1141 ctgcggcagc cgggttgggt cccgcaataa gagcagccga gaacaggcag gtgccgatcg
     1201 tcctggatat gatcgtcacg ctggccgcgc tacttttggt tttactgtgg tggcaacagc
     1261 ttgccgggat tcatgatgtc atgcggatcg acggcggcct tgatcgtacg catcagcgca
     1321 acggcggctt cgccgaactc caggttcagg aacttctgtt tgccgatgcc gatgccgtgc
     1381 tcaccagtgc atgtgccgcc catgtcgatt gctctctgaa ccatccgctc actcaagtcc
     1441 agtaccgtct tgtattccgc aggatcatcg gggtcgacca ggaaaacata gtgaaagttt
     1501 ccgtcgccga tatggcccaa cagaaacgaa ctgatgccgg tgcttttatg gacgtcggcc
     1561 cgcgtctcgc gaatgcagtc cgcgaggcgg gaaatcggca cgcagacgtc ggtcgcataa
     1621 acttttgccg ctgggcggac cgccattgcc gcataagccg ccgaatgccg ggcatcccac
     1681 attgctcgac gttcctcctc gaatgctgtc cattcgaagt cggagccgcc tagtgccgcg
     1741 cagatggtgc cgacggtttc cgcctgctcc ttcaccgacg cggggctgcc gtggaactcg
     1801 agaaaaagcg cgtccttgcc tggatagcag gttccggagt agcgattgat cgcctccatc
     1861 tgggcggcat cgagcagttc gacgcgggcg atgggtatgc cgcactgaat ggtctggatc
     1921 gcggtattga cggcctcgtc cacgtcgtcg aagtagaccc tgccggagga aatcgcctcc
     1981 ggcctgccgt gcagccgtaa ggtcagctcc gtgatgatgc cgagtgttcc ctccgaaccg
     2041 acgaacagcc gtgtgagatc gtaacccgcc gccgacttac gcgagcgacg ccccgtcgtt
     2101 acgatgctgc cgtccgccat caccgccttc agcgacagga cattttcgcg catggtcccg
     2161 tagcgcacgg cattcgtgcc tgatgcgcgc gtcgccgcca tgccgcccag cgaggcatca
     2221 gcacccgggt caatggggaa gaacaggccg ctatcgtgca ggtaccggtc gagttgtttc
     2281 ctggtaacgc ccgcctgcac cctgcaatcc atatcgtcag cattgacttc aataatcgcc
     2341 tgcattctcg atgtgtcgat acagagccct ccctggtggg caacagtccc gccttcgagt
     2401 ccggtgcccg tgccgaaggg gactacggga atgcggtagc ggtggcaaat cgcaagcgcc
     2461 cctgcaacgt cttcggtgct ctcggcaaaa cagacggcat cgggacgctt cgcggaatgc
     2521 cacgattcat catgtgaatg atggtcgcgc actgccattc cgctgctgaa tttttccccg
     2581 aggaggcttt gcagcgcatc gagagcagcc ttaagattct tcgaggtttt acttggcgcc
     2641 ggtttcctca tagatgtact catcgctctt tgctaccgta cgtggccagc cggagccaca
     2701 atcgggggag tcgtatcgtg ccgaaacatc cgggcggtat gttcaaagct atcggacctg
     2761 gcgtgttata cgcggcgacc gcggtaggcg catcgcacct cgttcagtcc acccgggcgg
     2821 gagccagcta tggctgcgcc ctggttctgc tgatcgccgt gactttcgtt gcgaaatacc
     2881 ctgcgttccg cttcagcgcg cagtatgcga gcgctacagg tcattcgctg ctgcacggct
     2941 accgatacca gggcacgtgg gctgtcgcga cctacattat catcgcgatt ggcacgatgt
     3001 tcgctgctgt accggccgta acgctggtca cggcaggttt ggccaaagtc gcttttggcc
     3061 tgcagatggg taccttcggc atctccgtca tcatcctggc agggtgcaca gtggttctga
     3121 ttgtgggcgg atatcacgtg ctggaccgcc tcgtaaaagt tctcatggtt ctactctcgg
     3181 tagcgacgat cgttgccacc gcgctcgtaa tcccgaacgt ggactgggag gtatcgggaa
     3241 cgattatacc aacgggactg gtcaggacgg acgtgttttt catcgtggca ctgctcggct
     3301 tgatgccctc ctccgtggat atttcggtgt ggcactccct gtggtcggtg gccaaagcac
     3361 gcgatacgga acacccgccg accctgaatc agtccctggc cgattttcac ctgggttatg
     3421 tcggagcctt cgtgctggcc ctgtgcttcg tactgctcgg cgcgggggtc atgcataact
     3481 cgggcttgca atttgagtcc ggtgcgaccg agttcgccgc gcagctcatc gagctttaca
     3541 agaaaaccct gggtgagtgg agcgggccgc tgatcggcat cgtcgccgtc gccgtgatgt
     3601 tttcgacggt gttgactggc ctggatggct atccccgtgc cgcggtaaac cttttacata
     3661 tatttgcaga agagaaattg cccagcgacg cctcccaggc ggacaagatc aaccggaaag
     3721 tgtacgcggc tgcgctgatc ctgctggtgc tcgggtcggt actcatcttg tatttcttcg
     3781 taacctcaca aaggctcgtc gtcgacattg cagcaacgat catgtttcta tttgcgccga
     3841 tcatcgcgtg gctgaatcac cgcgcaatga cgagtcccga gatcatgaca gcggcaaggc
     3901 cgggccgggg attgctcgtc ctgagccacc tcggcgtgtt ctggatggcc gccttttccg
     3961 tctactatct ttatcttcgg ctgtctggct gacttacatc ctgatctact ggttgatggg
     4021 caagttcagt cgcgacctgt caataacctg tccatccttg atgacttgta cgatctccct
     4081 ggcattgttg atgtctcggg aaggatctgc ccctaacaac acgaggtccg cgatttttcc
     4141 ttcctcgatt gaccccagct cccgctcctt cccaaggaaa atcgcgccgt tcaacgtggc
     4201 gatgcgaatg atctccagcg gtgcgattcc tgcgccggcc agcaattcga gctcccggtg
     4261 caaagccggt cccgttgtct ggtcggtgcc ggcagcgaga ataccgccag cctcgctgat
     4321 cttgagcagg ttctcctgag cgatggtcgt catgatctgc atccaccatg tccacttccg
     4381 ctcctggtat tccttgcgcc tgtcggtctt cagcgtctcg atttcatcgg ccgtcagggt
     4441 tgcccggtac aggggctcat caaggaactc cggatgctcg gccaggcggc tgtagttgtc
     4501 accgatcgtg agcgtcgtca ccatcg
//
LOCUS       DASUOM010000009         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1567983,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000009 DASUOM010000000
VERSION     DASUOM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567983"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /gene="pstC"
                     /locus_tag="VFY27_00145"
     CDS             <1..1202
                     /gene="pstC"
                     /locus_tag="VFY27_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5763951.1"
                     /translation="VWIVLEPPVITELVIKGLPEAQRTLPSGQLDLLLNSVRNLAAGD
                     PAQATDAVLRDAALSYQEYSRTSRRLLACMCLGLALIGGAVAWRRIAPDFRARNRVED
                     VLLALLLAASTIAILTTIGIVLSVLFEAIRFFREVPITEFLFGLNWSPQTAIRSDQVG
                     SSGSFGAVPLFTGTLLITAIAMFVSVPVGLMTAIYLAEYASARVRAYAKPLLEILAGI
                     PTVVYGFFAALTMAPAIRRAGEAIGLDVASESALAAGVVMGIMIIPLVSSLSDDAINA
                     VPRAMRDGALGLGSTRSEAMLHVILPAALPGIVGGVLLAVSRAIGETMIVVMAAGLAA
                     NLTLNPLEAVTTVTVQIVTLLVGDQEFDSAKTLAAFALGLALFVTTLVLNMIGLHVVR
                     KYREQYE"
     gene            1199..2485
                     /gene="pstA"
                     /locus_tag="VFY27_00150"
     CDS             1199..2485
                     /gene="pstA"
                     /locus_tag="VFY27_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5763952.1"
                     /translation="MRVPISGRDTRKAVEAGLRQRRRKEAVFRTFGLLATGVGIVFLG
                     VFFLSLFTQGASAFRQTFIELEIEYSEDELAPGGDLDLEYADYDALVRNALRQAFPEV
                     TDRRELRELVRLVSASAGFQLRDAVLRDPDLVGTRQRVLVPASSTVDMLAKGKVDRDL
                     VADQRSLSDQQINWVDQLEKRGNLQMKFNTGFFTNGDSRDPEMAGIKGALMGSFYMLI
                     VTIALSFPLGVAAAIYLEEFAPKNRWSDLIEVNINNLAAVPSIVFGLLGLAVFINWFG
                     MPRSAPLAGGLVLALLTLPVIIIAARASIQAVPPSIREAAFGIGASRMQVVFHHVVPL
                     ALPGMLTGAIIGMSRALGETAPLLMIGMVAFIVDIPAGFSDPATALPVQIFLWADSPE
                     RAFAERTSAAIIVLLGFLLLMNLAAIILRKKVDRQW"
     gene            2519..>2809
                     /locus_tag="VFY27_00155"
     CDS             2519..>2809
                     /locus_tag="VFY27_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763953.1"
                     /translation="MSAMSEREPVSVRPLPDDVPGKPVEPVVEARRKATNRRRQLEEM
                     SELMGKTVGKVRSDNPFMIAENVNVHYGDLHAIKNVTLEIGKEEVVALIGPSG"
BASE COUNT          473 a          850 c          894 g          592 t
ORIGIN      
        1 tggtatggat tgtgctcgag ccgccggtca ttaccgagct cgttatcaag gggctgccgg
       61 aggcacagcg tacgcttcct tcggggcagc tggacctgct gctcaacagc gtgcgcaatc
      121 ttgccgccgg cgatcccgcg caggctactg atgcggtgct tcgcgatgct gcactctcct
      181 accaggaata ctcgcgcacc agccgccgtt tgctcgcctg catgtgcctg ggcctcgcgt
      241 tgatcggggg cgcggtcgca tggcgccgta tcgcgccgga tttccgtgca cggaatcggg
      301 tcgaagacgt gctcctggcg ctgcttctcg ccgcgtcgac cattgccatc ctgacgacga
      361 tcggcattgt cctgtcggtc ctgttcgaag cgattcgctt cttccgcgag gtgccgataa
      421 cggagttcct gttcggcctc aactggagcc cccagaccgc catccggtcg gaccaggtgg
      481 ggtcgtccgg cagctttggc gccgtgccgc tctttaccgg aacccttctc atcacggcga
      541 tcgcgatgtt cgtctcggtg ccggtcgggc tgatgaccgc gatctatctc gccgagtacg
      601 cctctgcccg cgttcgcgct tatgcgaagc cgctgctgga gatactggcc ggcatcccca
      661 cggttgtgta cggattcttc gcggccctga cgatggcgcc tgcgattcgc cgcgcgggcg
      721 aagccatcgg gctcgacgtc gcgtccgaga gcgccctggc cgcgggtgtc gtgatgggca
      781 tcatgattat tccgctggtg tcgtcacttt ccgatgacgc gatcaacgct gttccccgcg
      841 ccatgcgcga cggggctctc gggctcggct cgacacgctc ggaggcgatg ctccacgtga
      901 tactgccggc ggcgttgccc ggtattgtcg ggggcgtttt gctggccgtg tcgcgtgcca
      961 tcggcgaaac catgatcgta gtcatggccg ccgggctcgc cgcgaatctc accttgaatc
     1021 cgttggaggc tgtcacgacg gttaccgtcc agatcgtcac gctgctggtc ggagaccagg
     1081 agttcgacag cgccaaaacc ctggcagcgt tcgccctggg gctcgcactg ttcgtcacga
     1141 cgcttgttct caacatgatc ggcctgcacg tggtacgcaa gtaccgggaa cagtacgaat
     1201 gagagtacca atcagcggcc gtgatacacg caaggccgtg gaagcaggcc ttaggcagcg
     1261 ccgccgcaag gaggctgtgt tccgcacctt cggcttactg gctaccggcg tcggtattgt
     1321 attcctcggc gtattcttcc tgagtttatt tacccagggc gcaagtgcgt tcaggcagac
     1381 gttcatcgag ctcgagatcg agtattccga ggacgagttg gcgccgggtg gcgatctcga
     1441 cctcgaatat gctgactacg atgcgctggt aaggaacgcg ctgcgtcagg cattcccgga
     1501 agtcacggat cggcgggaac tgagggaact ggtccggctc gtgagcgcct cagccggctt
     1561 ccagttgcgt gatgcggtgc ttcgtgatcc ggatttggtg ggaacccgcc agcgagtcct
     1621 ggttccggca tcctccacgg ttgacatgct ggcgaaaggc aaggtcgatc gcgacctggt
     1681 agccgatcaa cggtcactgt cggaccagca gattaattgg gtcgaccagc tcgagaagcg
     1741 cggcaacctg cagatgaagt tcaacaccgg cttcttcacc aatggcgact cccgagaccc
     1801 ggagatggcg ggcatcaaag gcgccctgat gggctcgttc tacatgctca tcgtgacgat
     1861 tgcgctgtca ttcccgctcg gcgttgcagc agcaatctat ctcgaggaat tcgcgccaaa
     1921 gaaccgctgg tccgatttga tcgaggtcaa catcaataat ttggccgctg ttccgtccat
     1981 cgtatttggc ctcctcgggc ttgccgtctt catcaactgg ttcggcatgc cgcgttcggc
     2041 acccctggct ggcggcctcg tgctggccct gctcacgctg ccggtgatca tcatcgccgc
     2101 gcgtgcatcg atccaagcag taccgccatc cattcgcgaa gcggcgtttg gcataggcgc
     2161 ctccaggatg caggtggtgt ttcaccacgt cgtaccgctc gcgctgccgg gcatgctgac
     2221 cggcgccatc atcgggatga gccgggcgct cggtgagaca gcgccgctgc tcatgatcgg
     2281 catggtcgcg ttcatagtcg acatccccgc aggtttttcg gatcccgcga cggcattgcc
     2341 ggtccagata ttcctgtggg cggacagtcc cgagcgtgcg tttgccgaac ggacgtcagc
     2401 ggcaatcatc gtgttgctcg ggtttcttct gctgatgaat cttgccgcga tcatcctgag
     2461 aaagaaggtt gacaggcaat ggtgatagtg gtttgcaaag aggtttgcga gtatgggaat
     2521 gagtgcgatg tctgagcgcg agcccgtcag cgtgcggccg cttccggacg acgtcccggg
     2581 caaacccgtc gagccggtgg tggaagcccg tcggaaggcg acgaaccgcc ggaggcagct
     2641 ggaagagatg tccgagctga tgggcaagac ggtaggcaag gttcgctccg acaatccgtt
     2701 catgatcgcc gagaatgtca acgtccatta cggcgacctg cacgcgatca agaacgtgac
     2761 actggagatc ggcaaggagg aggtcgtagc gctgatcggg ccatccggt
//
LOCUS       DASUOM010000010         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_481159,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000010 DASUOM010000000
VERSION     DASUOM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481159"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY27_00160"
     CDS             <1..345
                     /locus_tag="VFY27_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763954.1"
                     /translation="QFNDRLWHIRRNLRQAGEGLVETQRARRSVDTAMREEPAQFAAF
                     GERIEAIRPRIDSLQRRVDASVQQHKVFLQDVAVEELQAQKQRLETYTVQARFALAAI
                     YDMSSTVGNVVP"
     gene            342..3149
                     /locus_tag="VFY27_00165"
     CDS             342..3149
                     /locus_tag="VFY27_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5763955.1"
                     /translation="MRVFLSGVLLALGALIFGPAAADEEGTIATLEGNTVDIRPGGVI
                     AGSNEKARENYRGFLDLVSDDPVLRAEAMRRLGDLELEAAEAMQLSSNIEALDGAGFD
                     TPVSLFRQLLETYPDYRRNDSVLYQLARAHEVAGQTDDALRVLNELVERYPQTPHLEE
                     IQFRRGEMLFLRKDYESAEAAYQAVVGFGEGARFYEQSLYKLGWTQFKLARHEESLDP
                     LFDLLDRKIGGSILADGDDRLESLGRAERELVEDTFRVLSISFSYMKGPDSIATCLRR
                     RGSPEYGYVIFMNLGDLYLEKERYVDAAKAYEAFVLEDPHHPKAPLLQVEVIEAYKLG
                     GFPSQVLDGKKSFVERFRMDGEFWRRNSPEGNAAVKDHLKANLTDLAQYYHAEAQRDG
                     KRADYQQAALWYRRYLDYFPGEADSANTNFLLAEILFESGDFASATAEYERTAYDYPR
                     HAHSGEAAYAALISYRKHEESLEGSARRKWHREYLDSGLKFADTYPEHPESGAVLTTV
                     AEDLFDQQQFDLAITVAENAALKVPPLTDSLARTAWTVIAHSKFDLNRFAEAEAAYYK
                     LKALTPPGDKESRQQIDDRIASAIYKQGELARDGGDLEAAVAHFTRLRQAVPQSPIRE
                     TAEYDAAAALINLKAWDRAATVLQAFRRDWPDSKFAADVTQKLAVTYMQAGRSGEAAG
                     EFERIAEADSNTADVRREALWKAADLYRTGRNAVKEEAVLAKIVDRYPNPLSESIEAR
                     YRLLQLVEAGSNQKLREKRLQDIVRADRSAGAERTDRTRFLAARASIDLAEPVRARFL
                     NAKISQPLADSLKRKKSLMEDVLAAYGKAADYGIAEVTTAATYRLGEVYQQFSRDLMQ
                     SERPRDLDADALEQYDLLLEEQAFPFEEKAIELYEANASRTADGVFDEWVQRSFIALA
                     ELVPARYAKLERSEDVVTSLF"
     gene            3271..3783
                     /locus_tag="VFY27_00170"
     CDS             3271..3783
                     /locus_tag="VFY27_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5763956.1"
                     /translation="MATDVPPRILTLYEQAVAAMAGGDATEAELQFRAFLLQNPDYPG
                     AHVNLAILMAAKGDDIAAAEHISAALALDPGHPAALNQLGMLERRRGRFLEAESAYMK
                     AVTASPGYALAHYNLGVLNELYLQRLDNALEHFERYLEFGGDDEQVAKWVTDLKRRLG
                     TVERTANVTE"
     gene            3809..4219
                     /locus_tag="VFY27_00175"
     CDS             3809..4219
                     /locus_tag="VFY27_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763957.1"
                     /translation="MRRLLFSCAMAGVLAAAPASAQQAPEEATGAEGEREALEPRVSS
                     AALEEQAQVRAKEDGGERVIDRLELDRTEVTGNQELPKVLYIVPWQKPDPGDLVGRPV
                     NSLLDEVLAPLDREEFIRQVDYYSDLYGVAGEEE"
     gene            4224..4874
                     /locus_tag="VFY27_00180"
     CDS             4224..4874
                     /locus_tag="VFY27_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5763958.1"
                     /translation="MDVLGAIVRFFQEGGLFMYPILFVFALGLAIAVERWMYLTLSGA
                     SNRALWKKIVPFLKAGNFQEAATVTGKSTAAIASILTYGLYRVKSARRRDDVEKAMEE
                     SLMEVLPRLEKRTHYLATLANIATLLGLLGTIIGLIQAFTAVSNANPADKADLLSASI
                     SVAMNTTAFGLIVAIPLLLIHALLQTKTNQIVDSLEMASVKFLNAITERQLKPAGA"
     gene            4877..5386
                     /locus_tag="VFY27_00185"
     CDS             4877..5386
                     /locus_tag="VFY27_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5763959.1"
                     /translation="MLSSRRSGRRNSETAELNITVFMNLMVILVPFLLITAVFSRVAI
                     LELNLPGASDETVAPEEQTFQLEITVRESGIEVGDRNLGALGVYPSAAGGYDFEALST
                     KLAEIKKSYPAKTDASILLEADIPYDTLVQVMDRVRVSETVLGDAIVRTDLFPDISIG
                     DAPVRDGGA"
BASE COUNT         1117 a         1648 c         1765 g          923 t
ORIGIN      
        1 caattcaacg accggctgtg gcacattcgc aggaacctgc ggcaagccgg cgaaggcctg
       61 gtggagaccc agcgggcgcg gcgcagcgtc gatacggcca tgcgtgagga gcctgcgcag
      121 tttgcagcgt tcggcgaaag gatcgaggcg atccgcccga gaattgactc cttgcagcgc
      181 cgcgtcgatg cgtcggtcca gcagcacaag gtcttcctgc aggacgtcgc ggtcgaggag
      241 ctgcaggcac agaagcagcg cctggagacg tacacggtcc aggcgcgttt tgccctggcg
      301 gccatctacg acatgtcctc tactgtcggg aacgtagttc cgtgagagtc tttctcagcg
      361 gcgttctgct ggcgctcgga gcgttgatct tcggacccgc agcggcggac gaggagggca
      421 ccatagcgac cctcgaagga aatactgtcg atatacgtcc gggaggcgtg atcgcgggca
      481 gcaacgaaaa ggcgcgtgaa aactatcgcg gctttctcga tcttgtttcc gacgatccgg
      541 tactgcgcgc agaggccatg cgccggctgg gcgacctgga gctcgaggcg gcggaggcga
      601 tgcagctcag cagcaatatc gaagcactgg acggtgccgg cttcgacacc ccggtctccc
      661 tcttccggca gctgctcgag acgtacccgg actaccggcg caacgattcc gtgctttacc
      721 agctcgcaag ggcgcacgaa gttgccggcc agacggatga tgcactgcgc gtgctgaacg
      781 aactcgtcga acggtatccg cagacgccac acctggagga gatccagttc aggcgcggcg
      841 aaatgctgtt cctgcgcaag gattacgaat cggcggaggc agcctaccag gcggtcgtcg
      901 gtttcggcga gggtgcgcgt ttttacgagc aatcgcttta caagctgggc tggacacagt
      961 tcaagctcgc gcgccacgag gaaagcctgg accccttgtt cgacttgctg gaccgcaaga
     1021 taggcggatc catactggcg gacggggacg accgcctcga gagtctcggt cgcgcggagc
     1081 gggaactggt cgaggacacg ttccgcgtcc tgagtatcag cttctcctac atgaagggac
     1141 cggacagcat cgcaacctgc cttcgccggc gcggctcacc ggaatacggt tacgtcatct
     1201 tcatgaatct cggagatctc tacctcgaaa aggaacgtta cgtggacgcc gcaaaggcgt
     1261 acgaagcctt cgttctcgaa gaccctcatc acccgaaggc gccgctcctc caggtcgagg
     1321 tcatcgaggc gtacaagctc ggcgggtttc cgtcgcaggt gctggatggc aagaagagct
     1381 tcgtcgagcg tttccgcatg gacggcgaat tctggcggcg caattcgccg gagggcaatg
     1441 ccgccgtcaa ggatcacctg aaagcgaacc tgaccgacct cgcgcagtac taccatgcgg
     1501 aagcgcagcg tgacggcaag cgtgccgatt atcagcaggc tgccctgtgg tatcgcaggt
     1561 acctggacta cttccctggc gaggccgaca gtgccaacac caacttcctg cttgcggaga
     1621 tcctgttcga gagcggtgat ttcgcctccg cgaccgcgga atacgagcgc acggcctacg
     1681 actacccgcg acacgcgcac tccggcgaag cggcttatgc agcactcatt tcgtatcgca
     1741 agcacgagga atcgctcgag ggttcggccc ggcgcaagtg gcaccgggaa tacctggaca
     1801 gcgggctgaa gtttgccgac acctacccgg aacaccccga gtcaggcgct gtgctcacta
     1861 ctgttgccga agatttgttc gaccagcagc aattcgatct cgcgatcacc gtggcagaga
     1921 acgcggcgct gaaggttccg cctttaaccg acagcctggc gcgcacggca tggaccgtta
     1981 tcgcccactc gaagttcgac ctgaatcgct tcgccgaggc ggaagcggcg tattacaagc
     2041 tgaaggccct tacgccgccg ggcgacaagg agtcccggca gcaaatagac gaccggatag
     2101 catcggccat ctacaaacag ggtgaactgg cccgcgacgg cggcgacctg gaagcggcgg
     2161 tcgctcactt cacgcgactc cggcaggcgg tgccgcagtc gccgatacgc gagactgccg
     2221 aatatgacgc tgcagctgcg ctgatcaatc taaaggcatg ggacagggca gcgacagttc
     2281 tgcaggcgtt tcggcgcgat tggcccgaca gcaagtttgc ggccgacgtg acacagaagc
     2341 ttgccgtcac ctacatgcag gccggccgca gtggcgaggc ggccggggaa tttgaacgca
     2401 tcgcggaagc tgactcgaac accgcggatg tgcggcgcga ggctctgtgg aaggcagctg
     2461 acctgtacag gacgggacgc aacgcagtga aagaggaagc ggtgctggcg aagatcgtgg
     2521 atcgctaccc gaacccgctg tccgagtcga tcgaagcccg ctaccgcctg ctgcaactgg
     2581 tggaggccgg gagtaaccaa aagctgcggg agaagcgcct gcaggacatc gtccgcgcgg
     2641 atcgcagcgc gggcgcggag cgcactgacc ggactcgctt cctcgcagcc agggccagta
     2701 tcgacctggc agaaccggtt cgcgccaggt tcctgaacgc caagatttcc cagcccctgg
     2761 ccgacagcct gaagcgaaag aaatctttga tggaggacgt gcttgcagcc tacggcaaag
     2821 cggcggacta cggaattgcg gaagtcacta cggcagcgac ttacaggctc ggcgaagtct
     2881 accagcagtt cagccgcgac ctgatgcagt cggaaaggcc gcgggatctc gatgccgacg
     2941 cactggaaca atacgacctg ctcctcgagg aacaggcgtt cccattcgag gagaaagcca
     3001 tcgagctata cgaagcgaac gcgtcgcgaa cggcggacgg cgttttcgat gaatgggtgc
     3061 agcgtagttt catcgccctc gcggaactcg tgccagcacg ctacgcaaaa ctggagcgca
     3121 gcgaagatgt cgtcacctcg cttttctaag cgcagtgcag cctgcgcctg gagacgctgc
     3181 ctgctgccgc tcggtgccat cgcgttgctg ctgtccggct gcgcttccgc cccgtccgga
     3241 ggggattctg caacgcctgg ggaaggcacg gtggccacgg acgttccgcc gaggatactg
     3301 acactgtacg aacaggcggt tgccgccatg gccggcggcg atgccaccga ggcggaactg
     3361 caattccggg ctttcctgct gcaaaacccc gactatcccg gcgctcacgt gaacctggcg
     3421 atactgatgg cggcgaaggg ggacgacatt gccgccgctg aacacatcag cgccgcactg
     3481 gctctggacc ccgggcatcc cgctgccctg aaccagctcg ggatgctcga gcggcggcgc
     3541 ggccggttcc tggaggcgga gtccgcttat atgaaggctg tcacagccag ccccggctac
     3601 gccttggcac actacaacct gggcgtcctg aacgaactgt acctgcagcg gctggataac
     3661 gcgctcgagc acttcgagcg ttacctcgag ttcggcggcg acgacgagca ggttgcaaaa
     3721 tgggtgacgg acctgaagcg tcgtctcggc accgtcgagc gcactgcgaa cgtgacggaa
     3781 tgacggagtg gctgatatgc ggcgcccgat gagaagactg ctcttcagtt gcgccatggc
     3841 tggtgttctc gcggcggcgc cggcctccgc gcagcaagcg cctgaggaag caaccggagc
     3901 agagggcgaa cgggaggcgc tggagccgcg ggtatcctcc gccgcgctcg aagaacaggc
     3961 gcaggttcgc gcgaaagaag acggcgggga gcgcgtgatc gaccggctcg agctcgaccg
     4021 gacggaggtc accggcaacc aggaactgcc aaaggtcctg tacatcgtcc catggcagaa
     4081 gccggacccc ggcgacctgg tgggcagacc ggtcaactca ctgctggacg aggtcctggc
     4141 gccgcttgat cgagaggaat tcattcggca ggtcgattac tacagcgacc tgtacggcgt
     4201 tgcaggcgaa gaggagtagg taaatggacg ttttaggcgc catcgtgcgt ttctttcagg
     4261 agggcgggct gttcatgtac ccgatcctct tcgtgttcgc gctcggactg gcaatcgccg
     4321 tcgagcgctg gatgtatctc acactgtcgg gtgccagtaa ccgcgcgttg tggaagaaga
     4381 tcgtgccgtt cctgaaggcc ggcaacttcc aggaggcagc tacggtgacg ggcaagtcca
     4441 cggcggctat tgcctcgata ctgacctacg gcctttaccg ggtgaaatcc gcgcgacggc
     4501 gagacgacgt agagaaggcg atggaagaga gcctgatgga ggtattgccg cggctggaga
     4561 agcgcacgca ctaccttgcg acgcttgcga acatcgcaac gctactcggc ctgctcggga
     4621 cgatcatcgg cctcatacaa gcgtttaccg cggtctccaa tgcgaatccg gcggacaagg
     4681 cggacctgtt gtcggcgagt atttcggtcg cgatgaatac gaccgcgttc ggactgatcg
     4741 tggcgattcc gttgctgctg atccacgcgc tactgcagac gaagacaaac cagatcgtgg
     4801 acagcctgga aatggcgagt gtcaagttcc tgaacgccat cactgagcgc cagctgaagc
     4861 ctgccggagc ctgaccgtgt taagcagccg ccggagcggc cggcgcaaca gcgaaacagc
     4921 cgaactcaac atcacggtgt tcatgaacct gatggtgatc ctggttccgt tcctgctgat
     4981 tacggcggtg ttctcgcgcg tggcgatcct ggaactgaac ctcccgggcg cttccgatga
     5041 aaccgtggcc ccggaagagc agacgttcca gctcgagatc accgtacggg aatcgggaat
     5101 cgaagtgggc gaccgtaact taggggccct cggcgtctac cctagcgccg ccgggggcta
     5161 cgatttcgaa gccctcagca ccaagctggc agaaatcaag aagagttatc ccgcgaaaac
     5221 ggacgcctcg atacttctcg aggccgatat cccctacgac acgctggtgc aggtgatgga
     5281 tcgcgtgcgg gtgagcgaaa ccgtccttgg cgacgcgatc gtccgcaccg acctgttccc
     5341 ggacatatcc attggcgatg cgcctgttcg cgacgggggc gcctgaatgc agaagtcaca
     5401 acgcgcgaaa cgaatggaga ggcggcatca gcgcagcaaa ctcggcggcg ccc
//
LOCUS       DASUOM010000011         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_601304,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000011 DASUOM010000000
VERSION     DASUOM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601304"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..1096
                     /locus_tag="VFY27_00190"
     CDS             464..1096
                     /locus_tag="VFY27_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763960.1"
                     /translation="MKCHTPNSSCYFMVIGFVFALASCAGSTKVEKLYQDPAVRGQNF
                     NRILVVGIASEAGERRQMEELVSAQLAERIESAIPSYKRLGVASVVTQDALDMAAEAA
                     EADAILITHIVSVSTSADVREGRVDIKSECRGGNPADYFLYDHEELREPDSVSFAHEV
                     VVVSNLYDADTGTRLWTIQSTCVDRAELESVMADEARAIVRQLRRDGLIG"
     gene            1251..>2051
                     /locus_tag="VFY27_00195"
     CDS             1251..>2051
                     /locus_tag="VFY27_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5763961.1"
                     /translation="MERRLRDVAANVTVLDRDDIEQNLAMSVGDVFIYSPGIEAEGGG
                     QRFGAEGLNIRGIGGNRVALLIDGVPLSDQFDVGNFSNATREFLNAGLIQRIEVLHGP
                     ASALYGSSAIGGVVAATTPDPTDIAPGRGLGMGLQTAWRGADGSWQGTGVAAAAGARA
                     GVLLGGSLHDGGAADSAALSAQVDAREYRNRSALLKLVADDASGRSWRAGILHHDADV
                     ESDLKSMLGSNRFASTTALRGDDEYQLDLLNLALDFGAPGGMVDAGVWR"
BASE COUNT          386 a          608 c          669 g          388 t
ORIGIN      
        1 gctcagcgtt tgcgcgtcgt ggagcagccc cggcgtgccg ctccgcgccc acaatggaaa
       61 aatgccgggg aaagacaatc ccgctccggc caacaggacg gcgcgcagca gttgccgccg
      121 gttaatagtc gatgtcatca aggattgcct gccactcatc tactcctgga acgcgggtct
      181 tgcatgacgc ccggacatcc cggacatgca gacttaagga ggctgcacag gctcgcgcgt
      241 acgcgcgccg ttcagtccgg tccagattgt gcgatgaagc cgaggctacc agattatgca
      301 catcccacgt attcgatagc ggacaggcgc atacttggac cggggatttc gacgagatcg
      361 actgggacaa gttctggaac gaagggtgac acacccccct gctgtacgac ttcgccgatg
      421 caggcgatct ctccagtacc catacccctt tggagggatc gacatgaaat gccatactcc
      481 taacagctcc tgctatttca tggtgatcgg cttcgtgttt gcgcttgcct cctgcgccgg
      541 cagcacgaag gtggagaagc tctaccagga ccccgcggtc cgcggacaga acttcaaccg
      601 catcctcgtg gtgggcatag caagcgaggc cggggagcgc cggcagatgg aggaactggt
      661 gtcggcgcaa ctcgctgaac ggatcgagtc ggcaatacct tcatacaagc gactgggggt
      721 tgcatcggtc gtcacgcagg acgcgctcga tatggcggca gaagctgctg aggccgacgc
      781 gatcctgatc acgcatatcg tcagcgtaag cacctcggca gacgtcaggg aggggcgcgt
      841 ggacatcaaa tccgaatgcc gtggtggcaa tcctgcggac tatttcctct acgatcacga
      901 agaactgcgt gagcctgatt ccgtgtcctt cgcgcatgag gttgtcgtag tgagcaatct
      961 gtacgatgct gacaccggca cccgtctctg gactatccag tcgacttgcg tcgacagggc
     1021 ggagctcgag tccgtaatgg cggatgaggc cagggcgatc gttcgccagc tacggcgaga
     1081 cgggctgata ggctgacgag ggtcagggga atcgccggat gcgtgccttc tcccgaaagg
     1141 tttgttggac tgcggcggcg ctgctgttta tatcggggac gggcgccgct gaaacacgag
     1201 gcggatcggc cggccagccc atagatgaaa ttgtcgtcgt tgctcacaag gtggaacgac
     1261 gactccgcga cgttgcggct aacgttacag tgctggaccg cgacgacatc gagcagaacc
     1321 tggccatgtc tgtgggcgat gtgttcattt attcacccgg catcgaggct gaaggaggcg
     1381 gacagcgatt cggtgccgag ggcctgaaca tccgcggtat cggcggcaac cgggttgccc
     1441 tgctcatcga cggcgtaccg ttgagtgacc agttcgacgt tggcaacttc tcgaatgcca
     1501 cccgcgagtt tctcaatgcc ggactcatcc agcgcatcga ggtcctgcac gggccggcct
     1561 ctgccctcta cggcagttcc gcgatcggcg gcgtcgttgc ggcgacaacg cctgatccga
     1621 ctgatattgc tcctggccgg ggcctcggga tgggcctgca gaccgcgtgg cgcggggctg
     1681 acggcagctg gcagggtacc ggcgttgctg cggcagccgg tgctcgcgca ggcgtgctgc
     1741 ttggggggag cctgcatgac ggcggcgccg ccgactcggc agcattatcg gcgcaagtcg
     1801 acgcccgcga ataccggaac cgctctgccc tgttgaagct ggtcgccgat gatgcgagcg
     1861 ggcgcagctg gcgtgccggc atcctgcatc acgatgcaga cgtcgaaagt gacctgaagt
     1921 cgatgctcgg cagtaatcgc ttcgcctcga cgacggcact ccgtggtgat gacgagtacc
     1981 agcttgatct cctgaatctc gcactcgatt tcggtgcgcc cggtggcatg gtcgacgccg
     2041 gggtgtggcg c
//
LOCUS       DASUOM010000012         5442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_118217,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000012 DASUOM010000000
VERSION     DASUOM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118217"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFY27_00200"
     CDS             <1..217
                     /locus_tag="VFY27_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5763962.1"
                     /translation="YGRTLGMQSWRLRIETADGRQPNLKQASVRFFAALLSWLPLGLG
                     FWWQVLDRDGLAWHDRLSGTRLRHYPK"
     gene            complement(222..1301)
                     /gene="lptG"
                     /locus_tag="VFY27_00205"
     CDS             complement(222..1301)
                     /gene="lptG"
                     /locus_tag="VFY27_00205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX5763963.1"
                     /translation="MRELQVSVLTGYLLRTILSSTLLVLGVLLALAALFEFIGQLDDV
                     QGSYTLPMALLYAALRLPQLSFEMLPIAALIGSLLGLGGLANSSELVVMRTTGISVGR
                     LAGMVAVSGVVLTIATALIGEFIGPPMDYFSRTLRNEARFEQEDIDFGNAAWVKDGPV
                     ILHLERINTEFEFGGIYLFRFNEDHSLQSIARAENSGIDDSDQWILENVRETRFLDDG
                     VQAVQASVAVETFDLNSELLGITLVKPVSLSGRGLLSYIGYLKRNELTAERYETELWS
                     RIARTATVVIMPVLALGFVFGSLRSSGYGARLMLGVLIGLGYFLASEMLANSGQVFDL
                     NPAIVSWMPPIALLLIALFALTRVR"
     gene            complement(1318..2406)
                     /gene="lptF"
                     /locus_tag="VFY27_00210"
     CDS             complement(1318..2406)
                     /gene="lptF"
                     /locus_tag="VFY27_00210"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5763964.1"
                     /translation="MFRILDRYIFREIAQSWLAVTGVLLFILLTNQFARVLGDVAKDK
                     LPRDAVFQVIGLTALQYLTILVPIALFLSVMLALGRLYRDSEMPAMMACRIGPGGVYR
                     PLALLLVPLAIGVGWLAMDVGPRALTEIERIGAEARRQADLASIEAGKFTGDGADGAV
                     VYVERVPDPGEVERVFLQRRMSDGRVEVVVADRGEQQETDDPNTRFFVLFDGRRYEGV
                     PGTADFRVMEFAEHGIPYRLPVAEDIEQDPEAMSTIDLLTPRTLEEAAEIHWRIGVPL
                     STVVLAILAVPLSRSQPRQGRYGRLAIGLLVFIIYFNLLSAGKAWIEKGSMPVAAGLW
                     WAHGLMLLLALTLLAVQNGWYRRLRRRS"
     gene            2698..4197
                     /locus_tag="VFY27_00215"
     CDS             2698..4197
                     /locus_tag="VFY27_00215"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5763965.1"
                     /translation="MDYFTTTSAASRQKAGCLIVGIYESGLLSAAAAEIDTATGGAIK
                     RLAKSGDLSGELGECRTICSLKGVRADRVVIAGLGQKEKFGVAEFRQAMGAAVRAFKG
                     SKVYEVVNYLTIEDVKAASAYYLARYTVESIGNGLYDFEEMKSNRKNVKTPINRIGVA
                     LKDGSERSIAKKGAEHADAIVTAMTLAKDLGNLPPNICTPTYLARTAERLARQHDSIR
                     TQVINEAAMKRLNMHSLLSVSNGSAEPAKLIVMQYKGGDARDKPVVLVGKGITFDSGG
                     ISLKPGPAMDEMKYDMCGAAAVIATVAAIANLKLPINVNAIVPSSENLPSGTATRPGD
                     IVTSMSGLTIEILNTDAEGRLVLCDAITYSRRFKPSVIIDVATLTGACVIALGHHRTA
                     IMSNNDELARSIASAGDAADDRGWHMPLGEEYAQQLKSNFADFANVGGREGGAITAAA
                     FLGKFTEGLDWAHLDIAGTAWVSGSKKGATGRPVPMLTEFLLQRAGALP"
     gene            4220..4648
                     /locus_tag="VFY27_00220"
     CDS             4220..4648
                     /locus_tag="VFY27_00220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5763966.1"
                     /translation="MARIDFYLIAAADEPSRQIFACRLAEKAYRLQNTVHIQAADREH
                     ARQLDDLLWTFRDGSFVPHEVVDEERDAGDAPVTIGVGAAGARPVDLLINLSNDLPQQ
                     LDSFPRVAEIVTSDEDCRQQSRRRFADYRLQGHTLQTHEL"
     gene            4792..>5442
                     /locus_tag="VFY27_00225"
     CDS             4792..>5442
                     /locus_tag="VFY27_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5763967.1"
                     /translation="MEKTYDPREIERRLYQRWEENGWFAPSGSGAPYCIMIPPPNVTG
                     TLHMGHAFQDTIMDALTRYHRMMGRKTLWQPGMDHAGIATQMLVERQLNAEGTSRSEL
                     GRDRFVERVWKWKEESGGSIAHQLRRLGASVDWSRDRFTMDEGLSAAVTEVFVRLYDD
                     GLIYRGKRLVNWDPVLHTALSDLEVLQSEEAGQLWHFRYPLLDGSGHVIVATTRPET"
BASE COUNT         1152 a         1682 c         1680 g          928 t
ORIGIN      
        1 ttatggccga acgctcggaa tgcaatcatg gcgtctgcgg atcgagacag ccgacggccg
       61 gcagccgaat ctcaagcagg cgagcgtacg attttttgcc gcattgctgt cgtggctgcc
      121 gctggggtta ggattctggt ggcaggttct cgaccgggac gggttggcat ggcacgaccg
      181 gctctccggc acgcggctcc gccattaccc gaagtagcgc attagcggac gcgggtcagg
      241 gcgaacaggg cgatcaggag caaggcgatc gggggcatcc aggacacgat cgcggggttc
      301 aggtcaaaca cctgccctga attcgccagc atctcgctcg cgagaaagta cccgaggccg
      361 atcagcacac cgagcatcag ccgcgcgccg taaccggagg agcgcaacga accgaatacg
      421 aagccgagcg ccagcacggg catgatgacc acggtcgcgg tccgcgcgat gcgcgaccac
      481 agctccgtct cgtagcgctc tgccgtcagc tcgtttctct tcaggtaccc gatgtagctc
      541 agcagccctc ggccagagag gctcaccggc ttcaccagcg tgatcccgag cagttcgctg
      601 ttgaggtcaa acgtctcgac cgccaccgat gcctggacag cctgtacgcc atcatcgagg
      661 aacctggttt cgcgaacgtt ctcgaggatc cactggtccg agtcgtcgat tcccgagttc
      721 tcggcccggg ctatcgactg cagcgaatga tcctcattga agcggaacag gtaaatcccg
      781 ccgaattcga actctgtatt gattctttcg agatgcagga tgacggggcc gtctttcacc
      841 caggctgcat taccgaaatc gatgtcctcc tgctcgaagc gcgcttcgtt gcgcagggtg
      901 cgcgagaaat agtccatcgg cggcccgata aactcgccaa tcagcgccgt ggcgatggtc
      961 aacacaaccc cggacaccgc aaccatgccg gccaggcgcc caacggagat tccggtagtc
     1021 ctcatcacca ccagttcgct gctgttcgcg agacctccaa ggccgagaag agagccgatc
     1081 agggcggcga tgggcagcat ttcgaaagac agttgcggca gtcgcagggc cgcgtacagc
     1141 aaagccatcg gcaacgtata gctgccctgg acatcatcga gctgtccgat gaactcgaac
     1201 agcgcggcca gggccagcag cacaccaagg accagcaggg tactgctgag gatggtgcgc
     1261 aacaggtagc cggtcagcac gctcacttgc agttcccgca aaaagtgcga tccggcatca
     1321 agagcgccgc cgcaggcggc ggtaccagcc gttctgtacg gccagcagcg tgagcgcgag
     1381 caggagcatg aggccgtgtg cccaccacag gccggcggcg accggcatgc tgcctttttc
     1441 gatccacgcc ttgccggcgc tcagcaggtt gaagtagatg atgaatacca gcagcccgat
     1501 ggcaaggcgg ccataacggc cctgccttgg ctggctgcgg ctcagcggca ccgcgaggat
     1561 cgcgagcacg accgtggaca gcggcacccc gatgcgccag tggatctctg ctgcctcttc
     1621 caacgtacgt ggagtaagca ggtcgatggt gctcattgcc tccgggtcct gttcgatgtc
     1681 ctctgccacc ggcaatcgat acggaatgcc gtgttccgca aattccatca cgcgaaaatc
     1741 ggcagtgccg ggaacgcctt cgtagcggcg cccgtcgaac aggacgaaga acctggtgtt
     1801 cggatcgtct gtttcctgtt gttcgccgcg gtccgccacc actacctcca ccctgccatc
     1861 ggacattcgc cgttgcagga aaaccctctc gacctcgccc ggatccggca cgcgttccac
     1921 gtagaccacg gcgccatccg ccccgtcgcc ggtgaacttg cccgcttcga tgctcgcaag
     1981 gtctgcctgg cgccttgcct ctgcaccgat gcgttcgatt tccgtcaagg cgcggggccc
     2041 gacgtccatc gccagccacc cgaccccgat ggccagcgga accagcaaca gcgccagcgg
     2101 acgatagacg ccgccgggac cgatcctgca agccatcatt gccggcattt cgctgtcccg
     2161 gtacaacctg ccgagcgcca gcatcaccga caggaacagc gcgatcggca cgagaatggt
     2221 caggtactgc agtgccgtga gcccgatgac ctggaatact gcgtctcgcg gcagcttgtc
     2281 ctttgccacg tcgcccagga cgcgggcaaa ctggttcgtc aggaggatga acagcaggac
     2341 ccccgtcacg gcgagccagg actgggcgat ttcacggaaa atgtatctgt cgaggatgcg
     2401 aaacatctaa aacccggcgg cgccagcctg aatcctcgcg gatatcgtcc ggaaacccgt
     2461 taaccaaaag caattgaata agttagacta acggttttga tttgtccggg acagccccag
     2521 cgccaaatcg acgaccatcc acccgtggtg ggcgcttcgg tggacactgg tcgtggcggg
     2581 gccgacggcc aacaataata gaacgctcga gccggcaact ttcctccagt cgcgcgaaag
     2641 aactgagtgc actcggagac catcatgcgg gcgcaaggcg tcaaacaaag gcattttatg
     2701 gactacttca cgacaacgag tgcggcatcg cgccagaagg ccggctgcct catcgtcggc
     2761 atctacgaat cgggcctgct ttcggccgcc gctgccgaga tcgataccgc aaccggcggg
     2821 gccatcaagc gacttgcgaa gagcggcgac ctgtcgggtg agctcgggga atgccggacg
     2881 atctgttcgc tgaagggtgt ccgcgcggat cgagtggtca tcgcaggact cgggcagaaa
     2941 gagaagttcg gcgtcgccga gttccgccag gcgatgggag ctgccgtcag ggcgttcaag
     3001 ggaagcaagg tatacgaggt cgtaaattac ctgaccatcg aggacgtgaa agctgcgagc
     3061 gcctactacc tcgctcgcta caccgtggag tcgatcggca acggcttgta cgacttcgag
     3121 gagatgaaga gcaaccgcaa gaacgtgaag accccgatca acaggatcgg tgtggcgctg
     3181 aaagacggca gtgagcggtc gatagcaaaa aaaggcgccg agcacgctga cgcaatcgtg
     3241 acggcgatga cgctcgcgaa agatcttggg aacctgccgc cgaacatttg cacacctacc
     3301 taccttgcca ggacggctga acggctggcg cggcagcacg acagtatccg tacacaggta
     3361 atcaacgagg ccgcgatgaa gcgcctcaac atgcactctc ttctgtccgt gtcgaacggc
     3421 agcgccgagc cggcgaagct gattgttatg caatacaaag gcggcgatgc gcgcgacaag
     3481 ccggtggtgc tcgtaggcaa gggaatcact ttcgattccg gcggcatttc actgaagccc
     3541 gggccggcaa tggacgagat gaaatatgac atgtgcggtg cggcagccgt catcgccacc
     3601 gttgcggcca ttgcaaacct gaaactgccg atcaacgtga acgcgatcgt gccgagcagc
     3661 gaaaacctgc cgagcggcac cgcaacccgg cctggcgaca tcgtcacaag catgtccggg
     3721 ctgacgatcg agattctcaa caccgatgcc gaagggcgcc tggtgctatg cgacgccatc
     3781 acctattcga ggcgcttcaa accttcagtg atcatagacg tggcgacgct gacgggggcc
     3841 tgtgtcatcg cgctcggtca tcaccgcacg gcgatcatga gcaacaatga tgagctggcg
     3901 cgaagcatcg caagtgcagg cgacgccgcg gacgatcgcg gttggcacat gccgctcggc
     3961 gaggaatacg cgcagcagct gaagagtaac ttcgccgatt ttgcgaacgt tggcggccgc
     4021 gaaggtgggg cgattacggc agctgcattc ctgggcaagt tcaccgaggg tctcgactgg
     4081 gcgcatctcg acatcgccgg caccgcatgg gtgtccgggt cgaagaaagg cgccaccggg
     4141 cgccccgtac ccatgttgac cgagttcctg ctgcagcgcg caggcgccct gccctgatca
     4201 cagccagggt gaggaccgga tggcgcgcat cgacttctac ctgattgccg cagcggacga
     4261 accgtcgcgg cagattttcg catgtcgcct ggcggagaag gcgtatcgcc tgcaaaacac
     4321 cgtgcacatc caggcagcgg accgggagca tgccaggcag ctggacgatc tgctctggac
     4381 ttttcgcgac ggaagcttcg ttccgcatga agtagtcgac gaggagcgcg acgccggcga
     4441 cgcgcccgta acaatcggcg tcggtgctgc cggggcccgc ccggtcgatc tgctcatcaa
     4501 cctgtcgaac gacctgcccc aacagctgga ctctttcccg cgcgtcgcgg agattgtaac
     4561 ctccgacgaa gactgcaggc agcaaagccg caggcgcttt gccgactatc gcttgcaggg
     4621 acacaccctg cagacacacg aactgtgacc ggccggatga gacaacgctg ttagcgattc
     4681 tcgacgggaa ggaaaagcgt caccagctcc tgtggttacc ggatttgcat catcagcacg
     4741 aggctaaaac catgtcgtgc gtaacatcaa cgcgggtttt atagcagctc catggaaaag
     4801 acatacgacc cccgggaaat tgaacggcgc ctgtaccagc gatgggaaga gaatggctgg
     4861 ttcgcgcctt ccgggtccgg cgctccttac tgcatcatga ttcctccgcc gaacgtgacc
     4921 ggcaccctgc acatgggtca cgccttccag gacaccatca tggatgccct gacacgctac
     4981 caccgtatga tgggacgcaa gaccctgtgg cagcccggca tggaccatgc cggcatcgcg
     5041 acgcaaatgt tggtcgagcg gcaactgaat gccgagggta cgtctcgcag cgagctcggg
     5101 cgggatcgct tcgtggagcg ggtatggaag tggaaagagg aatccggcgg ttcgatcgcg
     5161 caccagttgc gacgcctcgg tgcatcggtg gactggagtc gcgaccggtt cacgatggac
     5221 gagggactat cggctgcagt caccgaagta ttcgtaaggc tatatgacga tggcctgatc
     5281 tatcgcggca agcgcctcgt caactgggat ccggtcctgc atacggcact gtcggacctg
     5341 gaagtgctgc aatcggaaga ggcggggcag ctgtggcact tccgctaccc gctcctggac
     5401 ggcagcggcc atgtcatcgt ggcgaccacg cgtcccgaga cc
//
LOCUS       DASUOM010000013         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_840450,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000013 DASUOM010000000
VERSION     DASUOM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840450"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFY27_00230"
     CDS             <1..207
                     /locus_tag="VFY27_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX5763968.1"
                     /translation="QKLNVVWVVFFATTGLLNIYIAYNFSEAFWVNFKVFGLLGLTIA
                     FMLAQSFWLFAKLDVASTQESGEK"
     gene            209..508
                     /locus_tag="VFY27_00235"
     CDS             209..508
                     /locus_tag="VFY27_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5763969.1"
                     /translation="MWYAILGEDVPESGKLRADARPAHLARLQQLVTEGRLLIAGPHP
                     AIDALDPGPAGFTGSLVIAEFPSLEDAQQWADEDPYVVAGVYARVAVKPFKAVLP"
     gene            505..819
                     /locus_tag="VFY27_00240"
     CDS             505..819
                     /locus_tag="VFY27_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5763970.1"
                     /translation="MTGSRREQIEAVLVDRFRPSEIRVKDQSHLHAGHAGAADGRGHF
                     DVYIVAEAFIDQSRVRRHQLVYKALADLLKSDIHALSIRALSPGEAASGRRDQATKTF
                     NR"
     gene            832..1614
                     /locus_tag="VFY27_00245"
     CDS             832..1614
                     /locus_tag="VFY27_00245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5763971.1"
                     /translation="MGNRFNLAVPAVAICAIAFAPSVHADTAFSVNGVDVDSMIFDVY
                     MESRLQKPAAQATAEEKAALQDELTSIYLLTTQPRADEIAKEPRVQAQLELQRRGILA
                     QATMQDFMSRNPASEDEIKAEYDRQVQNASDLQYKARHILVESQGAATDLIAQLDEGA
                     DFPELAMEHSTDSSASSGGDLGWFSPNQMVPPFSEAVVALEDGAYTSEAVQTQFGWHV
                     ILREESRKSEPPTLESVHDRIKQGLEQQKLQEYLAQLQELDN"
     gene            complement(1961..2239)
                     /locus_tag="VFY27_00250"
     CDS             complement(1961..2239)
                     /locus_tag="VFY27_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763972.1"
                     /translation="MVDGTVAGPGYIYADRVTVSSEQYVPGATQVFVTGIPSVVDRNL
                     GRVSMGSLVVDYTSSLAGGYPAAGAIWTFKGTQPNIRGLMVAGEAAAR"
     gene            2370..>2549
                     /locus_tag="VFY27_00255"
     CDS             2370..>2549
                     /locus_tag="VFY27_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763973.1"
                     /translation="MLISLTLPVEMSRILPVLISLTLPVEMSRILPVVISLTLPVEIS
                     RILPVLISLTRPVEMS"
BASE COUNT          540 a          773 c          730 g          506 t
ORIGIN      
        1 cagaaactca atgtcgtctg ggtcgtcttt ttcgcaacaa caggactgct caacatctac
       61 atcgcttaca acttctccga agcgttctgg gtgaacttca aggtattcgg tctcctggga
      121 ctgacaatcg cgtttatgct ggcgcagagc ttctggcttt tcgcaaaact cgacgttgcg
      181 tcgacccagg aatccgggga gaagtagcgt gtggtacgcg atcctgggtg aagacgtgcc
      241 ggagagcggc aaactgcgcg ccgatgcgcg gcccgcgcac ctcgcccgcc tgcagcaact
      301 ggtgacggaa ggccgtctac tcatcgccgg cccccatcct gccatcgatg cgctcgatcc
      361 gggcccggcc ggcttcacag gcagcctcgt gatcgcggag ttcccgtcgc tcgaagatgc
      421 acagcagtgg gctgacgaag acccttacgt cgttgccggc gtgtacgccc gtgtcgcagt
      481 caagccgttc aaggccgttt tgccgtgacc ggttcgcggc gggaacaaat agaggccgtc
      541 ctggtcgaca ggtttcgccc gagcgagata cgcgtcaagg accagagcca tttgcacgcc
      601 gggcacgccg gcgcggcaga cggacgcggc catttcgacg tgtatatcgt cgccgaggca
      661 ttcatcgatc agtccagggt acggcggcat caactcgtct acaaagccct ggcagatctc
      721 ctgaaatcgg acattcacgc cctgagcatc agggccctgt caccaggcga ggccgccagc
      781 ggccgtcgcg accaggcgac aaagactttc aatcgttgag gaacacaaac catgggtaac
      841 cgtttcaacc tggctgtgcc ggccgttgcg atctgcgcaa tcgcgtttgc gccatcggtg
      901 catgccgaca cggccttctc ggtcaacgga gtcgacgttg acagcatgat atttgatgtc
      961 tacatggaat cacggctgca aaagccggct gcgcaggcga ccgcggagga aaaggcggcc
     1021 ttgcaggacg aacttacgag catttacctg ctgacgacgc agcctcgcgc ggacgaaatc
     1081 gccaaggaac cgcgcgtcca ggcccagctc gagctgcagc ggcgcggcat cctcgcgcag
     1141 gcaaccatgc aggacttcat gtcgcgcaat ccggcatccg aggacgagat caaggccgag
     1201 tacgatcgcc aggtccagaa tgcctccgat ctccaataca aggcccggca tatcctggtc
     1261 gaaagccagg gtgccgctac cgatctcatc gcccagctcg acgaaggtgc ggacttcccg
     1321 gagctggcaa tggagcactc aaccgattcg tccgcctctt ccggcgggga cctgggctgg
     1381 ttctccccga accagatggt gccgccattt tccgaagctg tggttgctct cgaggatggc
     1441 gcctacacca gcgaggcggt acagacacag ttcggctggc acgtgatcct tcgcgaagaa
     1501 tcacgcaaat cggagccgcc gacgcttgag agcgtacacg acaggatcaa gcagggcctc
     1561 gagcagcaga agctgcagga gtacttggcg caactgcagg aacttgacaa ttagcatgca
     1621 caacttaacg taatactgaa aaaagggcgc acgtttgcgc ccttttcttt cccgcaaagt
     1681 tgatttgtga ttccgatcac atcatcgcgc cagctatcac cctaccattg cacgcgagtc
     1741 gcctctaatc gctcgggggt gactcctcca accctcgcat ctccttcatg gttgatacga
     1801 gaactaaagt cggcagggac gccgactttt ttttgtccaa agaatttttt ttgtccaaag
     1861 aaaaaggcta ccaagggtag cctttttcgc tcgagagaac tgcgcacaat gtccggttcc
     1921 gatgagcgat tcggaacaat tccctcgacg ccgttgcgaa ttaccgtgcc gcagcttctc
     1981 ctgcgaccat cagcccgcgg atgtttggct gggtgccctt gaacgtccat atcgcacccg
     2041 cagcgggata acctcctgca aggctcgacg tgtagtcgac taccaggcta cccatggaaa
     2101 cacgcccaag gttccggtcg acgactgatg gaatgccggt cacgaacacc tgagtagcgc
     2161 ccggaacata ctgctccgac gagacggtga cacgatcggc gtagatataa cctgggccgg
     2221 cgactgtccc gtcaaccatt atcatctgac cagcttgaag cccgcgaatt aatcgcgcgc
     2281 tcccgagtat ggtttggcca agtgcttcaa aggtattgtc gcgaccattt acctgggcga
     2341 ttggaccata aagcaagggc ttactgccgg tgctgatatc gctgacactg ccggtcgaga
     2401 tgtcccggat actgccggtg ctgatatcgc tgacgctgcc ggtcgagatg tcccggatac
     2461 tgccggtggt gatatcgctg acgctgccgg ttgagatatc ccggatactg ccggtgctga
     2521 tatcgctgac gcggccggtc gagatgtcc
//
LOCUS       DASUOM010000014         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_599625,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000014 DASUOM010000000
VERSION     DASUOM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599625"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFY27_00260"
     CDS             <1..680
                     /locus_tag="VFY27_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763974.1"
                     /translation="AEGDALSVEQVIDNMRAATASERARNAIQSLTTTADCTGPEGEF
                     ETTVTSIRPDTVYFRQASARAITELWSTPERTWGGNAGEEYQLLTPRVRDFIRAQEFH
                     LMIIDLESRFTDFELLGEREVEGQECLWVSMKDDAGGDASLCIRTDDWLPVELRLDRE
                     SATGPVAISFGDWWESGGITFFHSMRVIEEPDRVFTYDFVDISVNTFAYEMRVPPPEM
                     PKLRRGD"
     gene            732..1523
                     /locus_tag="VFY27_00265"
     CDS             732..1523
                     /locus_tag="VFY27_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5763975.1"
                     /translation="MKSMLLSLLAVAAFVYVCFCLYLYLFQRSFIYFPTASPKGVAAE
                     EMWLENEQETILVWRLHGSRPDAILYFGGNAEDVSYNTVEFRDWFPQYAVYLVNYRGY
                     GGSSGTPGEAALYSDAQAVFDSVAAKHATVSLIGRSLGAAIAVHLATRRDVARLALVT
                     PFDSLRSLASEFYPIFPTAILLKDTYDAIGLASRVRAPALVLVAEHDEIIPRQSSERL
                     AGALGGPHVIRTVEGTGHNTIGASPGYGRALREFMTLQSRSTSGR"
     gene            1520..1918
                     /locus_tag="VFY27_00270"
     CDS             1520..1918
                     /locus_tag="VFY27_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX5763976.1"
                     /translation="MSDAILADGVVLLHLAFVLFVIFGGLLVLRYPRFAFAHVPAFIW
                     GAYIEISGGLCPLTGVENRLRQKVGEGGYDAGFIEHYIYPVLYPPGLTRTMQLWLAAF
                     VLVLNGFIYGWILLRRQQNNPNGPASRNRT"
     gene            1967..2458
                     /locus_tag="VFY27_00275"
     CDS             1967..2458
                     /locus_tag="VFY27_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763977.1"
                     /translation="MKRLMENKGTRTGIYFGATSGVITTMGLIAGLYAGTDSLVAVLG
                     GILVVAVSDAMSDALGIHLAQEADPDSSSAHIWAATISTFLTKLLVALTFALPLMFLP
                     LGVAVLVSVGWGLIVIVLLSYYLARSQKVAVLPVVAEHLAIAVVVVVLAHFIGAWVQN
                     VFS"
     gene            2489..2827
                     /locus_tag="VFY27_00280"
     CDS             2489..2827
                     /locus_tag="VFY27_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX5763978.1"
                     /translation="MTEISWEDFEKVDLRAGTIIEARPFPEARKPAYKLTIDFGGTIG
                     IRKSSAQITDLYSLDELVGKQIIGVVNFPRKQVGPFMSECLVTGLVQDDGRVVLAVPD
                     KFAENGTRLG"
     gene            2847..>3031
                     /locus_tag="VFY27_00285"
     CDS             2847..>3031
                     /locus_tag="VFY27_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX5763979.1"
                     /translation="MSEHTATIIWQRGEQPFIDNAYSRGHEWTFDGGATVAASASPDV
                     VPLPMSVAENVDPEEAFV"
BASE COUNT          587 a          877 c          955 g          612 t
ORIGIN      
        1 gcgcggaagg cgatgcgctg tccgttgaac aggtgataga caacatgcgg gcggccaccg
       61 catcggaacg cgcgcggaat gcgatccaga gcctgaccac gaccgcagat tgcacgggtc
      121 cggaaggcga gttcgaaacg acggtcacat caatcaggcc ggacaccgtt tatttcaggc
      181 aagcgtcggc gcgtgcgatc acggaactat ggagtacccc tgaacgcacg tggggcggga
      241 atgcagggga ggaataccag ctgttgactc cgcgcgttcg cgacttcatc cgggcccagg
      301 aatttcacct gatgatcatc gacctggaat cgcgctttac ggatttcgaa ctgctcggcg
      361 aacgcgaagt agagggacag gagtgcctgt gggtctctat gaaggacgac gccggcggag
      421 acgcatctct ctgcatccgg acggatgact ggctgcccgt cgagctgcgg cttgaccggg
      481 agagtgcgac ggggccggta gccataagct tcggggactg gtgggaaagc ggtggaataa
      541 ccttttttca ttcgatgcgg gtgatcgaag aaccggatcg ggttttcacc tacgatttcg
      601 tcgacatttc cgtcaatacc tttgcctacg aaatgcgcgt accgccgccg gaaatgccga
      661 aactccggcg cggggattga accggcgagc aactacaata gcgggtgtaa gtccggtggc
      721 aggtaaacat catgaagtcc atgcttttat cgttgttggc ggtggccgcg ttcgtttacg
      781 tttgcttctg cctttacctt tacctgttcc agcggtcgtt catttacttc ccgacggctt
      841 ctccgaaagg cgtcgccgcc gaagaaatgt ggctcgagaa tgagcaggaa acgatactcg
      901 tctggcggct gcatggatcg cgtccggacg caatacttta ttttggcggc aatgcggaag
      961 acgtctcgta caacacagtc gaattccgtg actggttccc gcaatacgcg gtgtatctag
     1021 tcaactaccg gggttacggg ggtagcagcg gaacaccggg cgaggctgcg ctttacagcg
     1081 acgcgcaggc cgtcttcgat tccgtggcag cgaaacacgc aaccgtctcc ctcattggcc
     1141 gaagcctggg cgccgcgatt gccgttcacc tggcaacccg gagggacgta gcacgactgg
     1201 ccctggtgac gccattcgac agcctgcgaa gcctggccag cgagttctac ccgatatttc
     1261 cgacagcgat actgctgaag gatacgtacg acgccatcgg actggccagt cgagtgcgcg
     1321 ctcctgcact cgtactggtg gcggagcatg acgagattat tccgcggcaa agttccgaaa
     1381 gactcgctgg cgcgctcggc ggcccgcacg tcatccgaac cgtcgaaggc accggccaca
     1441 acacgatcgg cgcctctccc ggctacggcc gcgccctgcg ggagttcatg acgctgcaat
     1501 cgcgcagtac gagcggccgg tgagtgacgc aatacttgcg gacggcgtgg tcctcctgca
     1561 cctggcgttc gtccttttcg ttatcttcgg cggccttctc gtcttgcgct acccgcggtt
     1621 cgcattcgcg catgtgccgg cgttcatctg gggcgcatac atcgaaatca gcggaggcct
     1681 gtgccctttg accggcgtgg aaaaccgttt gcgccaaaaa gtcggcgaag gcgggtatga
     1741 tgccggcttc atcgaacact atatctaccc ggttctgtat ccgccggggc tgacgcgtac
     1801 gatgcaactc tggctcgcgg cgttcgtcct ggtgctgaac ggcttcattt acgggtggat
     1861 actgctgcgt cgccagcaaa acaacccgaa cggtccggca agccgcaatc gcacgtagtc
     1921 cgtgcggcgt cggcaagcgg cggctgcata ccaccactgg agattcatga aacgactgat
     1981 ggagaacaag ggcacgcgca cgggcatcta ttttggcgcc acgtccggtg ttatcacgac
     2041 gatgggactc atcgccgggc tttatgcggg gacggactcg ctcgttgcgg tgctcggcgg
     2101 catcctggtg gttgcggtgt ccgatgcgat gtcggacgcg ctgggaatac acctggccca
     2161 ggaggcggac ccggactcga gcagcgcgca catctgggcc gcgaccatct ccaccttcct
     2221 gacgaagctg cttgtcgcgc tgacgttcgc gctgccgctg atgttcctgc cgctcggcgt
     2281 tgccgtcctc gtttccgtcg gctgggggct cattgtcatc gtgctgttga gctactatct
     2341 tgcgcgatcg cagaaagtgg ccgtactccc ggtggtcgcg gagcaccttg cgattgctgt
     2401 cgtggtggtt gtcctggccc attttatcgg cgcctgggta cagaacgtat tttcctgatg
     2461 caggttcaac ctgcaggagc acgagaagat gacggaaatt tcctgggagg acttcgaaaa
     2521 ggtggatctg cgagcgggca caatcatcga ggcccggccg ttcccggagg cgcgcaagcc
     2581 cgcgtacaaa ctgaccatcg atttcggcgg cacgatcggt atccgaaaat cttcggcgca
     2641 gatcaccgat ctctactccc tggatgaact cgtcggcaag cagatcatcg gtgtcgtcaa
     2701 ttttccgcgc aagcaggtag gcccgttcat gtcggagtgc ctggtaacag gcctggtaca
     2761 ggacgacggc cgggtcgtac tggcggtacc ggataaattt gcggagaatg gcacgcgact
     2821 gggctaggca gcgtgaagca gaactgatgt cggaacacac cgcaacgatc atttggcaac
     2881 ggggcgagca gccattcatc gacaacgcct atagccgcgg ccacgaatgg acgttcgacg
     2941 gcggcgcgac ggtggcggca tccgcatcac ccgatgtcgt gccactcccg atgtcggtgg
     3001 ccgagaacgt ggatccggag gaggcgttcg t
//
LOCUS       DASUOM010000015         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1802760,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000015 DASUOM010000000
VERSION     DASUOM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802760"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="tilS"
                     /locus_tag="VFY27_00290"
     CDS             complement(<1..500)
                     /gene="tilS"
                     /locus_tag="VFY27_00290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5763980.1"
                     /translation="MTFDAGQLRRRLLELQGEVAVSRYLVGFSGGLDSTVLVHALKRA
                     VDDATPVIAVHINHGLHPDAGAWEAHCRHIAGTLNVGFLACHVDVSDHGGAGIEAAAR
                     EKRYAAMKELVQDGDVVLSAHHEEDQAETLLLNLMRGSGLAGLAAIGARQEFGPGTLI
                     RPMLKVS"
     gene            complement(497..1453)
                     /locus_tag="VFY27_00295"
     CDS             complement(497..1453)
                     /locus_tag="VFY27_00295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5763981.1"
                     /translation="MDLKFLDFEQPIAELEAKIDELRFVADDSEININDEIRKLKGKR
                     DALIKTIFSKLSAWQVAQVARHPMRPYTADYLEILAPDFQELHGDRMFADDPAIIGGI
                     GRLDGRAIMFIGHQKGRDTKERVRRNYGMPKPEGYRKAQRLMRLAEKYGMPIITFIDT
                     PGAYPGVGAEERGQSEAIAYSLYLMAGLRTPILSVVIGEGGSGGALAIGVGDRLLMLQ
                     YSIYSVISPEGCASILWKSADKAEEAAEAMRITASSLSEFGLVDEVLPEPLGAAHRDV
                     KGSAEVIRNALLKHLQDLDGQPLDTLLEHRQARLAGFGQFKE"
     gene            complement(1482..>2106)
                     /locus_tag="VFY27_00300"
     CDS             complement(1482..>2106)
                     /locus_tag="VFY27_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5763982.1"
                     /translation="VRLDAHYFVDAKLADLGSEPPPQSNHGHGDRGYGQSRREVTVAG
                     LVMDIRKRGNRVTVTLDDDSGRLEVSLFSEAFQEFRHLLSKDEIVVVNGTLRYDDFMA
                     AWQLNARKVSPIDRIIESRAQSMVLRLAPNGQGQRLLARLHDVLLPYRKGTCGVAVQY
                     TGSEAAARLDLGPEWTVRPSRELRDKLTELLGQDSVRLLYAPQREMI"
BASE COUNT          361 a          693 c          641 g          411 t
ORIGIN      
        1 gaaactttga gcatcgggcg aatcaaggtg ccgggaccga attcctgccg cgcgccgatt
       61 gccgcaagac cagccaggcc gctgccgcgc atcaggttca gcaacagcgt ctccgcctgg
      121 tcttcctcgt gatgtgcgct aaggaccaca tccccgtcct gcacgagttc cttcatggcg
      181 gcgtagcgct tttcccgtgc agcagcttcg atgccggcgc cgccatgatc gctcacgtca
      241 acgtggcacg cgaggaagcc gacgttcaac gtccccgcga tatgcctgca atgagcctcc
      301 cacgcgccgg cgtccggatg caatccatgg ttgatgtgca cggcaatgac aggtgtggca
      361 tcgtcgaccg cccgcttcag cgcgtgcacc aggaccgtcg aatccagccc gccgctgaac
      421 ccgacgagat atctcgacac agccacctca ccctggagct cgaggagccg tcgccgcagc
      481 tggccggcgt cgaacgtcat tccttgaact ggccgaaccc tgccaggcgg gcctggcgat
      541 gttcgagcag cgtgtcgaga ggctggccat cgaggtcctg caggtgcttc agaagagcgt
      601 tgcggatgac ctcggcgctg cccttgacat cccggtgcgc ggcacccagc ggttcgggca
      661 gcacttcgtc gacgaggccg aattcactca ggctcgatgc cgtgattcgc atggcttccg
      721 cggcctcctc cgccttgtcg gcgctcttcc acaggatcga ggcgcagccc tccggcgaaa
      781 tgacggaata aatgctgtac tggagcatca ggaggcgatc gccaacgccg atcgccaatg
      841 ccccgcccga accgccctcg ccgatgacga cgctcaggat cggtgtgcgc agccccgcca
      901 tcaagtacag gctgtaggct atggcttcgc tttgtccgcg ctcttcggcg ccgacgccgg
      961 gatacgcgcc gggcgtgtcg atgaaagtga tgatcggcat gccgtacttt tcggccagcc
     1021 tcatcagcct ctgcgctttg cgataccctt ccggtttcgg catgccgtag ttgcgccgca
     1081 cgcgttcctt ggtgtcgcgg cccttctggt gcccgatgaa catgatcgcc ctgccatcga
     1141 gacggccgat gccgccgatg atcgcggggt cgtccgcgaa catccggtcg ccgtgcagtt
     1201 cctggaagtc gggcgcgagg atctcgagat agtccgcggt gtacgggcgc atcgggtggc
     1261 gcgccacctg cgcaacctgc caggcactca gcttggagaa aatagtcttt atgagcgcgt
     1321 cgcgcttgcc cttcagcttg cggatttcat cgttgatgtt gatttcggaa tcgtccgcaa
     1381 cgaaacgcag ctcatcgatc tttgcctcga gctcggcgat cggttgctcg aaatccagaa
     1441 acttcagatc cattgaaatc cttcgtcgac ctcagttgcc attagatcat ttcccgctgt
     1501 ggagcgtaca gcagccggac gctgtcctgt cccagcaact ccgtcagctt gtcgcgcagc
     1561 tcgcggctag gccgcaccgt ccactccggc ccgagatcaa gtcgcgcggc ggcctcgctg
     1621 ccggtgtact ggacggccac accgcaggtg cctttgcgat acggcagcag cacgtcatgc
     1681 aggcgagcga gcagacgctg tccctggccg ttcggcgcga gcctcaagac catgctttgc
     1741 gcgcggctct cgatgatacg gtcgatcggc gacaccttgc gggcgttcag ttgccaggca
     1801 gccatgaaat cgtcgtaacg aagggtgccg ttcaccacga cgatctcgtc cttggaaagc
     1861 aggtggcgga attcctggaa ggcttcgctg aacaggctga cttcgagccg cccgctgtcg
     1921 tcgtcgagcg tgactgtgac gcgattgccg cgcttgcgta tgtccatcac cagtccggcg
     1981 acggtaacct cgcggcggct ctgcccatag ccgcggtcgc cgtgcccatg gttactttgg
     2041 ggcggaggtt cggaaccgag gtccgcaagc tttgcgtcaa cgaagtaatg cgcatccagg
     2101 cgcacg
//
LOCUS       DASUOM010000016         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_959771,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000016 DASUOM010000000
VERSION     DASUOM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959771"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..249
                     /locus_tag="VFY27_00305"
     tRNA            160..249
                     /locus_tag="VFY27_00305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:194..196,aa:Ser,seq:tga)
     gene            complement(252..680)
                     /locus_tag="VFY27_00310"
     CDS             complement(252..680)
                     /locus_tag="VFY27_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5763983.1"
                     /translation="MEIQELHRGRLIDHIQLVVRDLKASRRFYEAVFRALEIPVSGSA
                     EKHFWADELFISTTSSPDAAGELTGRNHIAFQARDHAMVDAFYRTGLEHGGRDNGAPG
                     LRAYHPGYYAAFLLDPDGNNIEAVHHGKVDRSAASVKITF"
     gene            complement(705..1115)
                     /locus_tag="VFY27_00315"
     CDS             complement(705..1115)
                     /locus_tag="VFY27_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5763984.1"
                     /translation="MPRITRVLETALYVDDLTRAKEFYARHLDLAQLFCDERMCAFAI
                     GESVLLLFVRGEGASPARTPGGTIPPHDGHGPLHVAFAIDKSDLDAWERRLDHAGIEL
                     ESRVDWPRGGKSIYFRDPDGHLLEFATPGLWANY"
     gene            complement(1120..1353)
                     /locus_tag="VFY27_00320"
     CDS             complement(1120..1353)
                     /locus_tag="VFY27_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763985.1"
                     /translation="MAVCDQCGNDYDKSFQVSKNGRTWTFDSFECAIQSLAPTCVHCS
                     CRIIGHGLEKDGAFYCCDHCAEKSGVTELRDRA"
     gene            1552..2331
                     /locus_tag="VFY27_00325"
     CDS             1552..2331
                     /locus_tag="VFY27_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763986.1"
                     /translation="MANGRRASKTDERAGASSMKPAVIIVGADKGGVGKTTISRTLLD
                     FMAARNLLARAFDAEHPRGSLKRFHPNVTEVIDITAVADQMKMLDTLVSSERKISVID
                     IRAGQLSNTLRAFTDIGFFDAVRSGEFRFALFHVLGPSVASLEEIADVLPFTGGKNYF
                     AVKNFINETSFFEWDPAVYDSYFKMAKGAVELNVPKLNEMAYEQVDLSGVPFSTFIKN
                     KNAQGHNANNSLVLRGYAKTWLKQVFDEYERVGLVDRLTDE"
     gene            2376..>2766
                     /locus_tag="VFY27_00330"
     CDS             2376..>2766
                     /locus_tag="VFY27_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763987.1"
                     /translation="MSETKKHHVLIVASLRPRTGKTLLARLLAEYLTLSGEPRIVFDT
                     DAAEPRLSAFFPGEAKVIDLERVPDQMKLFDTLVSDPGKSQVIDLTHRSFAKFFNLMR
                     DIGYADEAKAAGVEPVIFFIPDGSPASY"
BASE COUNT          545 a          826 c          865 g          530 t
ORIGIN      
        1 ctgaacccgg atggtagccc ggattcccct gctttcttgt atcagaacgc gcccggactg
       61 gggcaaaagt aggactcgcc ggagggcatg gttaactttg cctgatcttc agcggctgat
      121 gcttgcaatc gagggggcaa gacttcatct ggtcgcgacg gaggggtggc cgagtggttt
      181 aaggcagcgg tcttgaaaac cgccgtgcct gcaagggcac cgtgggttcg aatcccaccc
      241 cctccgccac gctagaacgt gattttcacg gacgcggcgc tgcggtctac cttgccgtga
      301 tggaccgctt cgatgttgtt tccgtccggg tcgagcagga acgcggcgta atagcccgga
      361 tggtacgcgc gcagaccggg cgcaccgttg tccctgccgc catgctctag cccggtgcga
      421 tagaaagcat cgaccatcgc gtggtccctt gcctgaaatg cgatgtggtt acggcctgtc
      481 agttctccgg ccgcgtccgg gctactggtg gtggagatga acaactcgtc cgcccagaaa
      541 tgcttttccg ccgagccgct cacgggaatc tcaagtgcgc gaaacacggc ttcgtaaaag
      601 cgccggctcg ccttgaggtc ccttacgacg agctggatgt gatcgatcag acggccacga
      661 tgaagttcct ggatttccat atcagcttcc cgtttctgcc ggacctagta gttcgcccac
      721 agaccaggcg ttgcgaattc gagcaggtgt ccgtcaggat cgcgaaaata gatgctcttg
      781 ccgccgcgcg gccagtccac ccgactctcc agctcgatgc ccgcatggtc gagcctgcgc
      841 tcccaggcgt cgagatcgct cttgtcgatt gcgaatgcca catgcaacgg accgtggccg
      901 tcatgcggcg ggatcgtgcc tccaggcgtc cgcgccgggg acgcaccctc gccgcgcacg
      961 aacaggagca gcaccgactc gccgatcgcg aaagcgcaca tgcgctcgtc gcagaaaagc
     1021 tgcgcgagat cgagatgacg cgcgtaaaat tctttcgccc gggtgaggtc gtcaacatac
     1081 agcgcggtct cgagcacgcg ggtgatccgg ggcatggtct caggcgcgat cgcgcaactc
     1141 ggtgacgccc gacttctcag cgcagtggtc gcaacagtag aaggcgccgt ctttttccaa
     1201 gccatgtccg atgatgcggc aggagcaatg gacgcaggtc ggagcgagcg actggatcgc
     1261 gcattcgaag ctgtcgaagg tccaggtccg tccattcttg gagacttgga acgacttgtc
     1321 gtagtcgttt ccgcattggt cgcagacagc catggctacc tccatcgatt cgacgttgga
     1381 ggaacggccg gtggcggcct ccggttccga agcgcaagcg cgccggggcc cgaccaccgg
     1441 ccttgatgct gagggaaaaa aacgacagtc tatcaagcac aaagggccgt tgcggggttt
     1501 ttacgcggcc cgccgggcgc cctcggtgcg tccgccaggg gagatcttcg cgtggcaaac
     1561 ggaagacgag cgtcaaagac ggacgaacga gcgggggcaa gcagcatgaa gccggcggtc
     1621 atcatcgtgg gtgccgacaa gggcggtgtc ggcaagacaa ccatttcgcg cactctgctc
     1681 gacttcatgg cggcgcggaa cctgctcgcc cgggcattcg atgcggaaca tccccgcggc
     1741 tcgctcaagc gcttccatcc gaacgtgacg gaagtcatcg acatcacggc cgtggccgac
     1801 cagatgaaga tgctcgatac gctggtcagc agcgagcgca agatcagcgt catcgacatt
     1861 cgcgccggac agctttcgaa cacgctgcgc gcattcaccg atatcggctt cttcgatgcc
     1921 gtgcgatcgg gcgaattccg cttcgcactc ttccatgtgc ttggcccgtc ggtcgcgtcg
     1981 cttgaagaga tcgccgacgt gctgccattc accggtggca agaactactt cgccgtgaag
     2041 aatttcatca acgagacgag cttcttcgag tgggatccgg ccgtttatga cagctatttc
     2101 aagatggcga agggcgccgt ggaattgaac gtcccgaagc tgaatgaaat ggcttacgag
     2161 caggtcgacc tctcgggtgt ccccttctcg accttcatca agaacaagaa cgcgcaaggc
     2221 cataacgcca acaactcgct cgtgctgcgt ggttacgcga agacctggct caaacaggtg
     2281 ttcgacgaat acgagcgcgt tggcctggtc gaccggctga ccgacgaata agagcgcggc
     2341 cgggcgcgga agtggagagt gtcccgggaa cggccgtgtc ggaaacgaag aagcatcatg
     2401 tcctaatcgt tgcgtcgctc cggccccgga ccggcaagac cctgttggcc cggttgctgg
     2461 cggaatacct gacattgtcg ggcgagccgc gcattgtctt cgatacggac gcagccgagc
     2521 cacgactgtc ggctttcttt ccgggcgagg cgaaagtcat cgatctggaa cgcgtcccgg
     2581 atcagatgaa actgttcgac acgctggtaa gcgatccagg gaagtcgcag gtgatcgacc
     2641 tcacccaccg gtcgttcgcg aagttcttca acctgatgcg cgatatcggt tatgcggatg
     2701 aagcgaaggc ggctggcgtg gagccggtga tttttttcat ccccgatggc agcccggcgt
     2761 cctatg
//
LOCUS       DASUOM010000017         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1316940,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000017 DASUOM010000000
VERSION     DASUOM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316940"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFY27_00335"
     CDS             complement(<1..533)
                     /locus_tag="VFY27_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5763988.1"
                     /translation="MLITPSRQNLNKIVVLNPKGGCGKTTLVTNLASYYAARGTLPAV
                     MDCDPQGSTMRWIEKRSPSQPPIHGIAAYKKTMQATRSWQLRVPLETTNLIVDSPAGL
                     THDNLREITRDATSILVPVLPSAIDIHAASRCIADLLLVAKIDRRDRKLAVVANRTRQ
                     NTRSFDKLMRFLDSLGIP"
     gene            885..>3554
                     /locus_tag="VFY27_00340"
     CDS             885..>3554
                     /locus_tag="VFY27_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5763989.1"
                     /translation="MRPDDARPFYVRLPGLFLSVISLATLAITPASLAQEASDAAAED
                     DEEVTEEIVVTGSRLKRDTYSSIAPLQVISGQVSREVGLIDAGEILQESTASSGQQID
                     LTFSGFVLDNGPGSSTISLRGLGEARTLVLLNGRRLAPAGVEGAPAAADLNLVPASLV
                     QQYDLLLDGASSVYGSDAVAGVTNIILRKDFTGLEVEAFTNIPEQGGGVENTLSLAWG
                     KNFDRGFFGIGGEYTRLEEVTLGDRRWTSPCERNIEIDEYGRIRTVDLFNQASLGMSA
                     NECSSLLLAGRAAFTFPRSLNAAGTGSVYYTPGTTNIGIPNFSESGIFGVGVDADGDG
                     QTDIDFRDYALESRQQEASLFPELDRTSLMAYGEYTLPGEMNITPFFEAQYNRREFFS
                     DSGAFQLFPSVPGTNPFSPCNPAAAGGVDCGLAYDALMNNPNYIADFVNFYGASPADF
                     GLGADGPSGPAPSQPIVSVDGDRTETVVEAQQIRAVLGLRGDLPMLNFGSMSNWTFEV
                     AGIYAKSDGDSSRMGIRGDRLNLAVGNYSTTGTPCENNVGAVLAADAAAGCVPVNMFA
                     PSLYEGVVGDFATDAERQYLFDSRDFDTEYTQTVLTTYVTGDVFELPAGGVSVGLGFE
                     QRDDEIKSIPDDVARDGLFFGFFSDGGATGEKYTREAFGEIELPLLADLPAVEELTVN
                     LSGRYTKDEFYGSATTYSYKVGYRPVNSLLLRATLGTSYRAPNLRENFLQNQTGFNNI
                     FDPCGIPEAALDPLGGGYNPALDTRDPVVLANCAAQGVDPTALDFGGFNTYSVEIARG
                     GALDLFEEESESWSAGFAWEQPFFEGFDLTFGATYYEIEIDNAIIEPTGQFIVNDCYA
                     NPAQNSTFCSRITRDADQRLNFLAAGFINRDNET"
BASE COUNT          687 a         1046 c         1084 g          737 t
ORIGIN      
        1 gggatgccga gcgaatccag gaagcgcatc aacttgtcga agctgcgcgt gttctgccgg
       61 gtgcggttcg caacgacggc gagcttccgg tcgcggcggt cgatcttcgc aacgagcagc
      121 agatccgcga tgcaacgcga cgccgcatga atatcgatcg cggaaggcaa cacgggcacc
      181 agtatgctgg tcgcgtcgcg tgtgatttcc cggaggttgt cgtgcgtcag accagccggg
      241 gagtcgacga tcaggttcgt ggtctcgagc ggcacacgca gctgccagct acgcgtcgcc
      301 tgcattgtct tcttgtacgc cgcgattccg tgtatcggcg gctgggaggg ggagcgcttc
      361 tcgatccagc gcatggtcga cccctgcggg tcgcagtcca ttaccgccgg caaggttccg
      421 cgggccgcgt agtagcttgc gagattggta acgagcgtgg tcttgccgca gccgcctttc
      481 gggttcagga cgacaatttt gttcaggttc tggcggctcg gcgtgatcag catattcccg
      541 gcaactgtgt gccaatcccg gttcgactgc gctgcgccgg ttcacacatt ggccgcagta
      601 gccagtggcg ggcgccgcgc gccgttttcg gcaatttcca gttattcgtc gtcccgcaac
      661 aacggtccgc gcaaaaacgc cgctgcaacg ccctgttttc gttggtttct tgccacagtt
      721 ggcgtctttc tggcggattc ccgtttacaa ggcgctgctt atttcgcatg attggccggg
      781 gacgaaaccg aggtgcaacc gggcgtccga attaaaaaat gggcaaacaa aaggcccgtg
      841 ctgccgtgac aggcaggcga acaagtgcat aagggggaaa gaccatgcga cctgatgacg
      901 ctcgtccgtt ctatgtacgg ctgcccggct tattcctttc ggttattagc ctcgctaccc
      961 tcgcgataac gccggcatcg cttgcccagg aagcgagcga tgccgccgcc gaggacgacg
     1021 aggaggtaac ggaagaaatt gtcgtcacgg gatcgcgact gaagcgcgac acctacagca
     1081 gcatcgcgcc tttacaagtc atttccggac aggtttcccg tgaggtcggc ctcatcgacg
     1141 cgggcgagat tcttcaggag tcgaccgctt ccagcggcca gcagatcgac cttaccttct
     1201 ccggattcgt gctggacaac ggcccgggtt cgtcgacgat cagcttgcgt ggcctgggcg
     1261 aggcacggac gctggtcctg ctgaatggcc gtcgcctcgc cccggcaggc gtcgaaggcg
     1321 cgccggcggc ggcagacctg aacctcgtgc cggcatccct ggtgcaacag tacgacttgc
     1381 tgctcgatgg cgcctcatcg gtctacggat cggatgccgt ggcgggtgtg actaacatca
     1441 tactccgcaa ggatttcacc gggctggagg tggaggcctt cacaaatatc ccggaacagg
     1501 gcggcggtgt ggaaaacacc ttgtcgctcg cctggggcaa gaacttcgac cgcggattct
     1561 tcggtatcgg cggtgagtac accaggcttg aggaagtgac tctcggggac cgcagatgga
     1621 ccagcccctg cgaacgcaat atcgaaattg acgagtacgg ccggattcgc actgtcgatc
     1681 ttttcaacca ggcttcgctc ggaatgtctg ctaacgagtg ctcgagcctg ctccttgccg
     1741 ggcgcgctgc tttcaccttc cccagatcgc tgaatgcagc cgggacagga agcgtttact
     1801 acacgccggg aaccaccaac atcggcattc cgaacttttc cgaatcgggc atcttcggtg
     1861 ttggggtgga tgcagacggt gacggccaga ctgacatcga cttcagggat tacgctctcg
     1921 agagccgaca gcaggaagcc agcctcttcc cggaactgga tcgcacgagc cttatggcgt
     1981 acggcgaata cactttgccg ggggagatga atatcacccc gtttttcgaa gctcagtaca
     2041 accgccgtga attcttctca gattccggtg ccttccagct gtttcccagc gtgccaggca
     2101 ccaacccttt cagcccctgt aacccggcgg ctgcaggagg tgtcgattgc gggcttgcgt
     2161 acgatgcgct gatgaacaac ccgaactaca tagcagactt cgttaatttc tacggtgcca
     2221 gtcccgccga tttcgggctc ggtgcggatg gtccctctgg cccggctccg tcgcaaccca
     2281 tcgtgagcgt cgacggggat cgtacggaga ccgtggtgga agcacagcaa attcgcgcgg
     2341 tgctcggatt gcgcggtgac ttgccgatgc tgaacttcgg ctccatgagc aactggacgt
     2401 tcgaggtcgc gggaatttac gcgaagtctg acggcgactc gagccgcatg ggtatccgcg
     2461 gcgatcggtt gaaccttgcc gtaggcaatt actcgaccac aggtacacct tgtgaaaata
     2521 acgtaggagc cgtgcttgcc gccgatgctg ccgcgggttg cgtgccggta aacatgttcg
     2581 ccccctcgct ttacgaaggg gtcgtcggcg atttcgcgac agatgccgaa cgacagtacc
     2641 ttttcgacag ccgcgacttc gataccgagt acacgcagac ggtcctgacc acctatgtga
     2701 caggagatgt gttcgagttg ccggcgggcg gcgtcagcgt aggcctgggc tttgagcagc
     2761 gtgatgacga gatcaagtcc attcccgatg acgtcgcgcg agacggattg ttcttcggtt
     2821 tcttctcgga cggtggagcc accggcgaga aatacactcg cgaggctttt ggcgaaatcg
     2881 aactgccgct tctggcggat ctgcctgctg tggaagagct gacggtgaac ctgtcgggcc
     2941 ggtacacgaa ggacgagttc tacgggtcgg ccactactta ctcctacaag gtcggctatc
     3001 gcccggtgaa ctccctgctg ttgcgcgcaa cgctcggaac gtcgtaccgg gcaccaaacc
     3061 tccgcgagaa ctttctccag aaccagaccg gcttcaacaa catcttcgat ccctgcggaa
     3121 taccggaagc cgccctcgat ccgcttgggg gtggctacaa cccggctctc gatactcgcg
     3181 acccggtggt acttgccaac tgcgcggcgc agggtgtaga ccccactgcg ctggatttcg
     3241 gtggcttcaa tacctacagc gtcgagattg caaggggtgg tgcgctggac ctgttcgagg
     3301 aggaatcgga gtcatggtca gccggtttcg cctgggagca gccgttcttt gagggtttcg
     3361 acctgacctt tggtgccacg tactacgaaa tcgaaatcga caacgcaatc attgagccga
     3421 caggacaatt catcgtcaac gactgctatg cgaatcccgc gcagaacagc actttctgca
     3481 gcaggatcac gcgagatgcc gaccagcgtc tcaactttct tgcagcgggt ttcatcaatc
     3541 gtgacaatga aact
//
LOCUS       DASUOM010000018         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_958815,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000018 DASUOM010000000
VERSION     DASUOM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958815"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="recF"
                     /locus_tag="VFY27_00345"
     CDS             complement(<1..661)
                     /gene="recF"
                     /locus_tag="VFY27_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX5763990.1"
                     /translation="MILKSFSCADFRCLASVQLELADRFNLVYGANASGKTSLLEAIA
                     YLGRGKSFRGAPIERLVRHGAIEFLLRGKVRLGSREIPVGVRNGRTGLELSVDGDRSG
                     GVAALASVLPLQVVDPDVHNLVAGGPDHRRKYLDWLTFHVEHGYLDLWRRFRRALRQR
                     NAALRDGATRELASWDAEFVELALSVDGFRQRALEKALPVLEAVGLRLLGDQVGFEYS
                     QG"
     gene            complement(714..1817)
                     /gene="dnaN"
                     /locus_tag="VFY27_00350"
     CDS             complement(714..1817)
                     /gene="dnaN"
                     /locus_tag="VFY27_00350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5763991.1"
                     /translation="MKLTAGREALLRPLQSVIGVVERRQTMPILANVLLVARNGQVAI
                     TATDLEVELVASAEVSVQTPGEVTVPGRKLFDICRALPENAEISISLSGEKLSVRSGK
                     SKFSLTTLPAAEFPSVEDINAGESLELPRESLARLLDKTHFSMAQQDVRYYLNGLLLE
                     SSKKHLRAVATDGHRLALCQVDLERSDAPEKQVIVPRKGILELQRLIGAGGDDNLTIE
                     LGSNHIRIQLAGIRFTSKLVDGRFPEYERVIPKDTGNQLSADRQLLKGALQRTAILSN
                     EKYRGIRLIIRKGNVVLQAHNPEQEEAEEEVEIDYDGEDIEIGFNVSYLQDALGAIDS
                     EQVTLSVVDGNSSCLMREPGNDDCKYVVMPMRL"
     gene            complement(2026..3357)
                     /gene="dnaA"
                     /locus_tag="VFY27_00355"
     CDS             complement(2026..3357)
                     /gene="dnaA"
                     /locus_tag="VFY27_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5763992.1"
                     /translation="MQAVTNLWNRCVVGLQEELSEQQFNTWIRPLHAVEDGNVLRLLA
                     PNRFVVDWLQQHYIERISQLIDGAGGASEVVIEVGSRKSQAPQGTAHTARPARPNPSS
                     VVVSRLNPVFTFSAFVEGKSNQLARAAAHQVGENPGKSYNPLFIYGGVGLGKTHLMQA
                     IGNVMLARDPGARVSYVHSERFVGDMVKGLQHNTISDFKRSYRSLDALLIDDIQFFAG
                     KERSQEEFFHTFNALLEGQRQIVLTCDRYPKEVAGLEERLKSRFGWGLTVAIDPPELE
                     TSVAILMSKAAGENAVLPEEVAFFIAKRIRSNVRELEGALRRVMATSQFTGRPISLDF
                     AKEALKDLLALQDRLVSVENIQKTVADYFKLRLADLLSKRRSRSIARPRQLAMALAKE
                     LTNHSLPEIGDAFGGRDHTTVLHACRRITALRETDKRVNEDYLNLLRTLTG"
     gene            3823..3957
                     /gene="rpmH"
                     /locus_tag="VFY27_00360"
     CDS             3823..3957
                     /gene="rpmH"
                     /locus_tag="VFY27_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5763993.1"
                     /translation="MKRTFQPGNLKRARTHGFRARMATKAGRLVLKRRRAKGRAQLTP
                     "
BASE COUNT          862 a         1289 c         1115 g          824 t
ORIGIN      
        1 aaccctggga atactcgaaa cccacctggt cgccgaggag tcgaaggccg accgcttcca
       61 gcactggcag cgccttttcc agggcccttt gccggaagcc atcgacggac agggcaagtt
      121 ccacgaactc ggcgtcccag ctcgccagtt cccgggtcgc accgtcacgc agggccgcat
      181 tgcgctgtct cagagcccgc cggaagcgcc gccaaagatc caggtaccca tgttccacgt
      241 ggaacgtcag ccagtccagg tacttgcggc ggtgatcagg cccacctgcc accaggttgt
      301 gaacgtcagg atccacgacc tgcaggggca ggacactcgc aagtgctgcc actccgccgc
      361 tgcgatcccc atccacgctt agctcgagcc ccgtgcgccc gttgcggacg cctaccggga
      421 tctcacggga gcccaatcga actttgccgc gcaggaggaa ctcgatagcc ccgtgtcgca
      481 cgagacgttc aatcggcgcg ccgcgaaagg atttgccacg cccgaggtag gcgatcgcct
      541 ccagtaggct tgttttgccg gaagcattgg cgccatacac caggttaaaa cggtcggcga
      601 gctccagctg gacagaggcg aggcagcgaa aatccgcgca ggagaacgat ttgaggatca
      661 aggaatcccg aaacgcgatg ctagaaccct gtcaactcga cgccggcaga atactaaagc
      721 cgcatcggca tgacgacgta cttgcagtca tcgttacccg gctcccgcat caggcagctg
      781 gaattaccgt ccaccaccga cagggtcacc tgctcggagt cgatcgcccc cagggcatcc
      841 tgcaggtaac tgacattaaa cccgatctcg atgtcttcgc cgtcgtaatc gatctctact
      901 tcttcctccg cctcttcctg ctccgggttg tgcgcctgca ggaccacgtt ccccttccgg
      961 atgatgaggc gaatgccccg gtatttttcg ttcgacagga tggccgttcg ttgcagcgcc
     1021 cccttgagca gctggcggtc ggccgacaac tggttgccgg tatctttcgg gataacgcgc
     1081 tcatattcgg ggaaacggcc gtcaaccagc ttcgatgtga accggatacc cgccaactgg
     1141 atacgaatgt ggttgctgcc gagttcaatc gtcaggttgt catctccacc ggcgccgatc
     1201 aggcgctgaa gctccaggat gcccttccgc ggaacgatca cctgtttctc cggcgcgtcc
     1261 gagcgctcga ggtccacctg gcacagcgcg agccggtgcc cgtccgtggc cacggcgcgc
     1321 aggtgcttct tgctgctctc cagcagcaac ccgttcaggt aatacctgac gtcctgctga
     1381 gccatcgaaa agtgtgtctt atccagcaac ctggccagcg attcccgggg cagctcgagg
     1441 ctctcgccgg cgttgatatc ctcaacggac ggaaactccg cggccggcag cgtggtcagg
     1501 ctgaacttgc tcttgcccga ccggaccgaa agtttctcgc cgctcaagct tatggagatc
     1561 tccgcgttct ccggcagggc tcggcaaatg tcgaacagct ttctcccggg caccgtcact
     1621 tctccggggg tctgcacgct cacttcggcg ctcgccacga gttcgacttc gaggtccgtg
     1681 gcggtgatgg ctacctggcc attcctggca accagcaaga cgtttgcgag tatcggcatc
     1741 gtctgccgtc gctccacaac accgatcacc gactgcaacg gcctcagcaa tgcctcccgg
     1801 cccgcggtta acttcatcga ttccacctgt taataaatat tggaaatatt tcttgctttg
     1861 ttattattag tgaccagagg tcctgtggat aacttatttt tttcttttga gatcaatgta
     1921 ttacccaccg atcgatcggc cgcaaaagcg tgtacgatcc gcaagctacc tgtgcatctc
     1981 ctgtgggtaa gtacaaggcc gaaacttctg caccgcttat ccacattatc ctgtcagagt
     2041 tcttaacaaa ttcaagtaat cttcgttaac ccttttgtcg gtttcccgca acgcggtaat
     2101 tcgccggcag gcgtgcagca ccgtggtgtg atcccgcccg ccgaaagcat ctcctatttc
     2161 cggcaggctg tgattcgtca actctttggc caaggccatg gccagctggc gtggcctcgc
     2221 aatcgagcgg cttcggcgct tcgataacag gtcggcgagg cgcagtttga aataatcagc
     2281 gactgtcttc tgaatatttt caacagacac gaggcggtcc tgcagcgcaa gaagatcctt
     2341 caaggcctcc ttggcaaagt cgaggctgat aggtcgcccg gtgaactgtg acgtcgccat
     2401 gaccctgcgc aaggcaccct cgagttcgcg gacgttcgaa cgaatgcgct tcgcaatgaa
     2461 aaatgccact tcctccggca gcaccgcgtt ctctcccgcc gccttgctca tgagaattgc
     2521 gacggatgtt tccagctccg gcggatcgat ggccactgtc aatccccagc cgaagcgcga
     2581 cttcagccgt tcctcgagac ccgccacttc cttggggtaa cggtcacagg taagcacgat
     2641 ctggcgttgt ccttccagga gcgcgttgaa ggtgtggaaa aactcttcct gcgagcgctc
     2701 cttcccggcg aaaaactgga tgtcatcgat cagcagggca tcgagcgaac gatacgagcg
     2761 cttgaaatcc gagatcgtgt tgtgttgcag gccctttacc atgtcgccga cgaagcgctc
     2821 cgagtgcacg taactcacac gcgcaccggg atctctcgca agcatcacgt tgccgatcgc
     2881 ttgcatgagg tgggtcttcc cgagcccgac accgccgtaa atgaacaggg gattgtagga
     2941 cttgccgggg ttctcgccga cctggtgcgc cgcggcccgc gcaagctggt tgctcttgcc
     3001 ctcgacaaac gccgagaaag tgaaaaccgg attcaaacga ctcacgacca ccgaagatgg
     3061 gttcggccgc gccggccgcg ccgtgtgcgc cgtcccctgg ggcgcctggc tcttgcgcga
     3121 gcccacttcg atgacgactt cgctggcgcc gcccgcgcca tcaatcagtt gcgagattcg
     3181 ctcaatgtaa tgctgctgca gccaatcaac tacgaaccgg ttcggcgcaa gaagtcgaag
     3241 tacgttcccg tcctcaacgg cgtgcaacgg ccgtatccag gtattgaact gttgctccga
     3301 cagttcctcc tgcaaaccga cgacgcagcg attccagaga tttgtcacag cctgcaacga
     3361 ggtcctcgtt cgatacacaa gtgagaacgc gaaacgatca aggccaaaaa gttgacgcca
     3421 aaaagttcaa gcttggaata gtgaatggca gatggcgatg agccgcgcag tctataacgt
     3481 ccccaaaacg ttcgccagag aacagaatac acctgtccgc ggacatccgc agataaaagc
     3541 gaagcgttaa aattcgcaga ctgttctcac tacgttcccc ggacttcgct ggttgcacgg
     3601 atcgcggatg aggctttcca atcgcagccc gggatcccgc ttttcctgcg aatccatggc
     3661 ggcgcttgct gccactggct taaggctcgg ttgacagaaa ttccctcccc aagtacgctt
     3721 gccgcccact ttttgcccgt ggcgagaggc ctcgctacgc aattcctgaa gtaaaacttt
     3781 tcggggctca accttgaggc tccaggaaca ggtcagacga caatgaagcg cacatttcag
     3841 ccaggcaact tgaagagagc gagaacccat gggttccggg cacgcatggc aaccaaagcc
     3901 gggcgcctgg tactcaaacg gcgacgcgcg aaagggcgcg cgcaactgac accctgactg
     3961 ttcgctaacc tgttcgcaca gcattactcc gcaataccca acaaaaacgg acgcatcgga
     4021 caaaacgcgg caggacgaag acagcctgat tccgagccag ctgaaaggca gttccgcccc
     4081 gatttcggcg
//
LOCUS       DASUOM010000019         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_231704,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000019 DASUOM010000000
VERSION     DASUOM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231704"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFY27_00365"
     CDS             <1..137
                     /locus_tag="VFY27_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222645.1"
                     /note="transfers the N-acyl diglyceride moiety to the
                     prospective N-terminal cysteine in prolipoprotein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5763994.1"
                     /translation="DAHMGDDGYLVGWLTTGQLLSAPMIVAGAILLFLAYRRPTAATA
                     "
     gene            159..953
                     /gene="thyA"
                     /locus_tag="VFY27_00370"
     CDS             159..953
                     /gene="thyA"
                     /locus_tag="VFY27_00370"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5763995.1"
                     /translation="MRQYLDLMRRVRETGDRKEDRTGTGTLSVFGHQMRFDLGRGFPL
                     VTTKKLHLRSIVHELLWFLSGDSNIRYLKDNGVRIWDDWADEHGDLGPVYGVQWRSWP
                     APDGATHDQIATVMRQLRETPDSRRILVCAWNVAEIDRMALPPCHTLFQFYVANGRLS
                     CQLYQRSADVFLGVPFNIASYALLTHMFAQQADLGVGEFIWTGGDCHLYVNHLEQADL
                     QLAREPLPLPRLAIRRRPPSVLDYRFEDFEILNYESHPHIPAQVAV"
     gene            973..1464
                     /gene="folA"
                     /locus_tag="VFY27_00375"
     CDS             973..1464
                     /gene="folA"
                     /locus_tag="VFY27_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HEX5763996.1"
                     /translation="MHVSLIAAVAENGVIGRDGKLPWHLPDDFRWFKAQTLGKPVVMG
                     RRTWESLGRPLPGRQNIVLTRQSDYAAPGATVVRSPEAALAAAGDAEEVMVIGGGDVF
                     GMFLPRATRVYLTRVNAVVEGDAWFPEFEGGDWWLVSREHHGTDARHRYEFEFCVYER
                     VDK"
     gene            1485..1991
                     /locus_tag="VFY27_00380"
     CDS             1485..1991
                     /locus_tag="VFY27_00380"
                     /inference="COORDINATES: protein motif:HMM:NF021482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEX5763997.1"
                     /translation="MSFRFKCHTCGEIHEGMPTFGADRPLCYYEIPKEERENRCQSSN
                     DDCVIDDKWFFVRGCIDIPVHGADDDFSWGAWVSLSEDNYLTWRDNFGKAKRSHVGPF
                     FGWLNAWLKPYPDTMNLKTMVHLRDDGIRPYIEIEPSDHPLSVEQRNGISIERLSEIY
                     SIMMHDDK"
     gene            2208..>2424
                     /locus_tag="VFY27_00385"
     CDS             2208..>2424
                     /locus_tag="VFY27_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5763998.1"
                     /translation="MAHPSSLVAGNCYFLVHYYDRDLLVPNVSTLVFRESRVDEETGH
                     RLWLFDEPTSSAEVSDAGGSIAVGLPDS"
BASE COUNT          465 a          710 c          758 g          491 t
ORIGIN      
        1 ccgacgcgca catgggcgac gacggctacc tggtcggctg gctcaccacc ggccagctcc
       61 tgagcgcacc gatgatcgtc gcgggcgcga tcctgttgtt cctcgcgtac cgccggccga
      121 cggcagccac ggcctgatgc gaatgcggga ttgacggcat gcggcagtac ctcgacctca
      181 tgcgccgcgt acgcgagacc ggcgaccgca aggaagaccg caccgggacc ggcacgctga
      241 gcgtcttcgg ccaccagatg cgcttcgatc tcggcagggg cttcccgctc gtcaccacga
      301 agaagctgca cctgcgctcc atcgtgcacg aactcctgtg gttcctctcc ggcgacagca
      361 acatccggta tctcaaggac aacggcgtgc gcatctggga cgactgggcg gacgagcacg
      421 gcgatctcgg gcccgtctac ggcgtgcagt ggcggagctg gcccgcgccg gacggcgcaa
      481 cgcacgacca gatcgccacc gtgatgcgcc agctccgcga gaccccggac tcgcggcgca
      541 tcctcgtctg cgcctggaac gtcgccgaga tcgaccggat ggcactgccg ccctgccaca
      601 cgctgttcca gttctacgtc gcgaacggca ggctgtcctg ccagctctac cagcgcagcg
      661 ccgacgtctt cctgggcgtg ccgttcaaca tcgcttccta tgcgctgctg acgcacatgt
      721 tcgcgcagca ggcggacctc ggcgtcggcg aattcatctg gactggcggc gactgtcacc
      781 tgtacgtgaa tcacctggag caggccgacc tccagctcgc gcgagaaccg ttacccttgc
      841 cgcggctcgc catccgccgc cgcccgccat cggtcctcga ctaccgcttc gaggatttcg
      901 aaatcctgaa ctacgagtcg cacccgcaca tcccggcgca ggtggcggtg tagaccagag
      961 cgtccgcgac gcatgcatgt cagcctgatc gcggccgtgg cggaaaacgg cgtcatcggc
     1021 cgggacggca aactcccctg gcacctgccg gacgatttcc gctggttcaa ggcgcagacc
     1081 ctcgggaagc ccgtcgtcat gggacgcagg acctgggagt cgctgggacg gccgctgccg
     1141 gggcggcaga acatcgtgct gacgcggcag tcggactatg ccgcgcccgg ggcgacggtg
     1201 gtgcgttcgc cggaggcggc gcttgccgcg gcgggggatg cggaggaggt gatggtgatc
     1261 ggcggcgggg acgtgttcgg gatgttcctg ccgcgggcga cgcgggttta tctgacacgc
     1321 gtgaatgccg tggtcgaggg ggatgcgtgg tttccggagt tcgagggcgg tgactggtgg
     1381 ctggtttcca gggagcatca cgggaccgac gcacggcaca ggtacgagtt cgagttttgc
     1441 gtctatgagc gggtggataa gtagaatttc ccgatagttg agcaatgtcg tttcgattca
     1501 aatgccatac ctgtggtgaa attcacgaag gaatgccgac ctttggtgcg gatcgaccac
     1561 tctgttatta cgagattccc aaggaagagc gcgagaaccg ttgtcagtca agcaacgatg
     1621 attgcgtgat tgacgacaag tggttttttg ttcgaggctg catagatatt cctgtccacg
     1681 gcgcggacga cgatttttct tggggcgctt gggtctcact cagtgaggac aattacctga
     1741 cttggcggga taattttggg aaagccaaga gatctcatgt tggccctttt tttggctggc
     1801 taaacgcttg gctaaagccg taccccgata cgatgaattt gaaaacgatg gttcatctcc
     1861 gagatgatgg gattcggccc tacatcgaga ttgagccaag cgaccacccg ttgtcggttg
     1921 agcagcgaaa cggaatttct atagaaagac tctcggaaat ctactcgatc atgatgcacg
     1981 acgataagta gttccgattg gcttgccaac gtttggttct ggccgttgag cgacaattct
     2041 ggagcagacg actacgggtc cgctcctcga atgagctgcc cctggacgtc ggttatgtcg
     2101 gctatgcatc ccttagaagt cagtcgcctg atattatccg gagactgcag gtgacccttt
     2161 gcggacaccg gtctagccga tatttgagtg aaaacagatc aaaccaaatg gctcacccaa
     2221 gctcactagt agcgggcaac tgctactttc tcgtccacta ttacgacaga gatctgttgg
     2281 ttccaaatgt ctctacttta gttttcaggg aatcgagagt tgacgaggaa acaggccatc
     2341 gactgtggtt atttgacgag cctacaagca gcgccgaggt ttccgatgcg ggaggttcaa
     2401 tcgctgtcgg gttgccagat tctc
//
LOCUS       DASUOM010000020         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_597669,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000020 DASUOM010000000
VERSION     DASUOM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597669"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..785)
                     /gene="tmk"
                     /locus_tag="VFY27_00390"
     CDS             complement(123..785)
                     /gene="tmk"
                     /locus_tag="VFY27_00390"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5763999.1"
                     /translation="MAMAQGKFITVEGGEGVGKSTNIDFICELIRSRGHDVVETREPG
                     GTPLAERIRGMLLEHGDEDLPDETELLLFFAARSLHLHNLIRPALRSGTWVVCDRFTD
                     ATRAYQGSGRGLDISRIEWLAEWVHGDLNPHLTILLDAAPHIGMKRAGERGEADRLET
                     EQAEFHLRVREGYLRQAATEPKRFAVVDADQPLPAVQGAIEAALGRLLDDNDPEDVRV
                     SN"
     gene            complement(766..1824)
                     /gene="mltG"
                     /locus_tag="VFY27_00395"
     CDS             complement(766..1824)
                     /gene="mltG"
                     /locus_tag="VFY27_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5764000.1"
                     /translation="MKRILYTGAVVAAVAAIALVILAWQALRFLDAPLNLPAEGLTYE
                     IAPGTAFAAVSNDLGRRGVISHPRMFRWYARLTGDASRIHAGEYRIERGTTPPELLGR
                     FVSGDVQLYSFTIVEGWTYRELLAGLKRHDAIVPTLEPDEWTELLEMLGAEATHPEGL
                     FLPETYRFAKGTHDVQILQRSYEMMSRTLEEEWARRVPDLPLSTPYEALILASIIERE
                     TAREDEREKIAGVFVRRLQKGMRLQTDPTVIYGIGPDFDGNLTRRHLQTSTPYNTYTM
                     SGLPPTPIALPGRAAIRAALNPAPGAELYFVATGLPDGSHRFSVTKAEHDAAVAEYLS
                     RLRKSRITATDANGNGAR"
BASE COUNT          343 a          656 c          634 g          378 t
ORIGIN      
        1 ggcggcgcgc ttgcctaccc cggcgctgcc ggtcagcagg accgcatgcg cgggccggcc
       61 acgggcctcg gcgccatgcc acgcctccgc cagcggcgca agccagggta actcatgcaa
      121 catcagttgc ttacccgcac atcttcaggg tcgttatcat caaggaggcg acccagcgcg
      181 gcttcgatag ccccttgcac cgccggcagc ggttggtccg catcgacgac cgcaaagcgc
      241 ttcggttcgg tggcggcctg ccggagatat ccttcgcgga cgcgcaggtg gaattccgct
      301 tgctccgtct ccagccggtc cgcctcgccc cgctctccgg cccgcttcat cccgatgtgc
      361 ggcgcggcat ccaggagtat tgtcaggtgc gggttcaggt cgccgtgcac ccattccgcc
      421 agccattcga tacgcgagat atcgagcccg cggccactgc cctggtaggc ccgcgtcgcg
      481 tccgtaaagc ggtcgcagac aacccatgtg ccagagcgca acgccgggcg aatcaggttg
      541 tggaggtgca gggatcgagc ggcaaaaaac aacagcaact ccgtttcatc cggcaggtcc
      601 tcgtccccgt gttccaggag cattcctcgg atgcgctcgg cgagaggcgt gccaccgggc
      661 tcccgcgtct ctacgacgtc atggccgcga ctccggatga gctcgcagat aaagtcgatg
      721 ttggtcgact tgccgacacc ctcgccacct tccacggtga tgaatttacc ttgcgccatt
      781 gccattcgca tccgttgccg tgattcgcga cttgcgcagg cgcgaaaggt attccgcaac
      841 ggcggcatca tgctctgcct tggtaaccga aaaccggtga ctgccgtcag gaagcccggt
      901 ggcaacgaaa tagagctctg ccccgggagc gggattcagc gcggcgcgga tggcagccct
      961 gcccggcaac gcgatcggtg tagggggcag ccccgacatg gtgtaggtgt tatatggagt
     1021 gcttgtctgc aagtggcggc gcgtcaggtt gccgtcgaaa tccgggccta tgccatagat
     1081 taccgtgggg tccgtctgca ggcgcattcc cttctgcagg cgccggacga acacgccggc
     1141 gatcttttcc cgctcgtctt cgcgggccgt ctccctctcg atgatcgagg cgaggataag
     1201 cgcttcgtag ggtgtcgaca ggggcaggtc cgggacccgt cgggcccact cttcctccag
     1261 cgtacggctc atcatttcgt aagaccgttg caggatctgg acatcgtgag tgccttttgc
     1321 gaaacggtag gtttccggca ggaacagccc ttcaggatgt gtcgcttccg ctcccagcat
     1381 ctcgagcaat tctgtccatt cgtccggctc gagcgtcggc acaatggcat catggcgttt
     1441 cagtcctgcc agaagctcgc ggtaggtcca cccctccacg atggtgaacg aatacagttg
     1501 cacgtcgccc gaaacgaaac gtcccagcaa ctcaggcgga gtggttccgc gttctatgcg
     1561 gtactcgcct gcgtggatcc tgctggcatc tccggtcagc ctggcgtacc agcgaaacat
     1621 gcgggggtga ctgatcacgc cgcgccggcc caggtcgttg ctgacagcgg cgaacgcagt
     1681 gccgggcgct atctcataag tgaggccttc cgcgggcaga ttcagcggcg catcgagaaa
     1741 cctcagggcc tgccaggcca ggatgaccag tgcgatggcc gcaaccgcgg cgacaactgc
     1801 gcctgtgtaa agtattcgct tcatgttgga cccggtcagt gtcgcatatt gcgcatactg
     1861 cggcgcgtcc agcgggacgt ggcggggccg gatcgccgcc ggcgcggtct gccgcaaggt
     1921 tcggctatca cctccgcggt ggcactagcc ccggcggctc aggccggccg gcggaatatc
     1981 aggctgccgt tcgttccgcc aaaaccgaat g
//
LOCUS       DASUOM010000021         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_836480,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000021 DASUOM010000000
VERSION     DASUOM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836480"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..515
                     /locus_tag="VFY27_00400"
     CDS             105..515
                     /locus_tag="VFY27_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5764001.1"
                     /translation="MTGTSYVMDPKWNEDAPIYRQLRDRVVAMILEGVLSDGDALPSV
                     RNVAAEYRLNPLTVLKGYQELVDEGLVEKKRGRGMFVTDGARKNLLKDERQRFVEKEW
                     PKIAATIERLGLDAKELLAGVGQAGASSQGKNND"
     gene            508..1365
                     /locus_tag="VFY27_00405"
     CDS             508..1365
                     /locus_tag="VFY27_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5764002.1"
                     /translation="MTSLLSARNVSKSFGSVRAVDDVSFDIGKGKIMGLIGPNGAGKT
                     TLLKAVLGLTDCEGSLSVLGLDPFRQRKELMQNICFIADVAVLPRWIRVNQLLDFVEA
                     VHPRFSRARAEELLKRTKILSRSKIRELSKGMVTQLHLSIITAIDAKLLVLDEPTIGL
                     DIIFRKEFYGNLLNDYFDKERTILITTHQVEEIENLLTDVMFINDGRIVLHASMDALP
                     ERYVELMTSGENAERARQLNPIFERDVFGKKVLTFEGIARDKLHGLGELRTPGIADLF
                     VAKVKGAAA"
     gene            1362..2285
                     /locus_tag="VFY27_00410"
     CDS             1362..2285
                     /locus_tag="VFY27_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEX5764003.1"
                     /translation="MIANQVALIRRELWEHRSIYVTPLVIALIVSVMTITGQAVVSAF
                     DQAVDIAIIGATNLGPAERAAGITVLMTGVASLLILAMWVLTVFYCLDALYAERKDRS
                     ILFWRSLPCTDAETVVSKLLTAAIVIPLITFAVIVITHLVVLAIASAWLGFQGADAWH
                     LIWRAAPLANNWTGTLIVLLALPLWLAPFIGWLLFVSAFTRRSPLLVAFLPIVILPML
                     EKSLVGTSAFFDAFFVRTGSMPLFRGVTTGDIFSYQGDEWSLNGEVPSGLLSLVDLPG
                     FLASGSLWVGLVVCGLFVTAAIYVRRYRDES"
BASE COUNT          460 a          729 c          768 g          500 t
ORIGIN      
        1 cggccggaca ccatgagccg ggcgtcccgc ggttgtgctg ctaacatagg gcccggcacc
       61 ggcggctata tccgcgtgtt gcctgcgccc gccgacaaca acgaatgaca ggcaccagct
      121 acgtaatgga tcccaaatgg aacgaagatg ctccgatcta ccgtcaactg cgagatcggg
      181 tggtcgcaat gatcctggaa ggggtattga gcgacggtga cgccctgcct tcggtgcgca
      241 acgtagcggc agaataccgg ctgaatcccc tgactgtcct caagggttac caggaactgg
      301 tcgacgaggg actcgtagag aagaagcgcg gccggggcat gttcgttacc gatggcgccc
      361 gcaagaatct gctcaaggat gagcgtcagc gattcgtcga aaaggaatgg ccgaagattg
      421 cggcaacgat cgagcggctg gggctggatg cgaaggagct actggctggc gttggtcagg
      481 caggcgcttc gtcgcagggg aaaaacaatg actagcctcc tgagcgcgcg caacgtgtcg
      541 aaatcattcg gttccgtgcg ggctgtggac gacgtcagct tcgatatcgg aaagggcaag
      601 atcatgggcc ttatcggccc gaatggcgcc ggcaaaacga cgctgctgaa ggcggttctg
      661 ggcctgaccg attgcgaagg cagcctttca gtgctggggc tggatccgtt tcgccagcgc
      721 aaggagctga tgcagaacat ctgctttatt gcggacgtgg ccgtactgcc gcggtggatc
      781 cgcgtgaacc agctgctcga cttcgtcgaa gccgtgcatc cgcggttttc gcgtgcgcgc
      841 gccgaagagc ttttgaaacg gacgaaaatc ctgtctcgat cgaaaattcg cgaactgtcg
      901 aaaggcatgg tgacgcaact ccacctctcg atcattaccg caatcgacgc gaagctgctg
      961 gtcctggatg agccgacgat cggtctcgac atcatctttc gcaaggaatt ctatggaaac
     1021 ctgctgaacg actatttcga caaggagcgc acgatcctca tcacgactca ccaggtcgag
     1081 gaaatcgaga acctccttac cgacgtcatg ttcatcaatg atggccggat cgtcctccat
     1141 gccagcatgg acgccttgcc ggaacgttac gtggaactga tgacgtcggg ggagaacgcg
     1201 gaacgggcgc ggcagctgaa cccgatattc gagcgcgacg tattcggcaa gaaagtactg
     1261 accttcgagg gcattgcccg cgacaagctg catggactcg gcgaattgcg cactccgggg
     1321 atagccgatc tcttcgtcgc caaggtcaag ggagccgcag catgatcgcc aaccaggtag
     1381 cgctgatcag gcgcgaacta tgggaacacc ggtcgatcta cgtcacgccg ctggtgatag
     1441 cgctgatcgt gtcggtaatg acgatcaccg gccaggccgt ggtatccgca ttcgaccagg
     1501 ccgtggatat cgcgattatc ggtgcgacga acctcgggcc cgcggagcgc gcggccggaa
     1561 taaccgtgct gatgaccgga gtggccagcc tcctgatcct ggcaatgtgg gtgctgacgg
     1621 tcttctactg cctggatgca ctgtacgccg agcgcaagga ccgcagcatc ctgttctggc
     1681 gctcgctccc gtgcaccgac gctgaaacgg tcgtgtcgaa gctgctgacg gccgccatcg
     1741 tcataccgtt gattaccttc gccgttatcg tcattaccca cctcgtggtc cttgccatcg
     1801 cgagtgcctg gctcgggttc cagggggcgg atgcctggca cctgatctgg cgcgcggcgc
     1861 cgctcgccaa caactggacc ggaacgctca ttgtcctcct ggcgctgccg ctgtggctgg
     1921 cgccgttcat tggctggttg ttgttcgtct ccgcgttcac gcgcaggtct ccgctgctgg
     1981 tggcgttcct gccgatcgtc atcctgccca tgctggagaa gagcctcgtt gggaccagcg
     2041 ccttcttcga tgctttcttt gtccgtaccg gatcgatgcc gctgttcagg ggcgtgacca
     2101 ccggcgacat attctcctac cagggagacg agtggagcct caacggcgag gtaccctccg
     2161 gtttgctgtc gctcgtggac cttcccgggt tcctcgccag cggctcgctg tgggtcgggc
     2221 ttgtcgtgtg cggactattc gtcaccgcgg ccatttacgt tcgccgctac agggatgaga
     2281 gctgatcgtc ccgccgcagc ctgttacgct ggcttaccct gggttcagtc cctgcgttgg
     2341 tcgaagccga cgcggtccag tccgtgtttt cgtacctttc cccgcatctg gtgatagctc
     2401 aaaccgagga gcgccgccgc agcgcgctgg ttgaagcggg ccttctccag cgcggta
//
LOCUS       DASUOM010000022         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1800113,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000022 DASUOM010000000
VERSION     DASUOM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800113"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFY27_00415"
     CDS             complement(<1..280)
                     /locus_tag="VFY27_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5764004.1"
                     /translation="MPVLYRASLGYLTRHPWQLALALVGICVGVAVMVAVDLANESAR
                     RAFLSSMDAVNGEATHQVIGGPAGIDELLYVDLRVSGGLRNIAPVVEGY"
     gene            complement(292..993)
                     /locus_tag="VFY27_00420"
     CDS             complement(292..993)
                     /locus_tag="VFY27_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5764005.1"
                     /translation="MNDANATAHIRIRNLSRSYQEGTRRHVVLASLNADFSHGETIAL
                     RGRSGSGKSTLLNLISGIDEPDEGEIQIDGATITAMSERERTLYRRRNIGFVYQAFNL
                     VPTLTAADNVRLVLELNDVPGTEANQRVSDLLAAVGLGDRHATYPDVLSGGEQQRVAI
                     ARALAHKPSLLLADEPTGNLDDVTAADVLSLLDNLVRAQGGTMIIATHSAVVAAFCDR
                     RFELREGLLAPTNAH"
     gene            1055..2011
                     /locus_tag="VFY27_00425"
     CDS             1055..2011
                     /locus_tag="VFY27_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5764006.1"
                     /translation="MIDALMLVAGLALLLKGADFLVAGASEVAERLGISHLVIGLTVV
                     AFGTSMPEVVVTLISGLGGHPDLAIGNILGSNIANILLVLGIAAIIRPLPVNDSTVVS
                     EIPFSLTAALLVGFLANAALFSSKPELSISHVDGAILLFFFFLFGIYIYNITRKQAVP
                     FEERAERKPLLKPVTMLLVGVGALWLGGDWVVGSAISIARNLGISDSLIGLTIVAVGT
                     SLPELMASAVAAYRNQPDIAVGNVVGSNIFNLLWVLGIASLITELPFEVVSNADLVMV
                     IVASTLLLLALVSSRATCVLRVHGVIFVLTYGAYLTYLIRRG"
     gene            2155..3648
                     /locus_tag="VFY27_00430"
     CDS             2155..3648
                     /locus_tag="VFY27_00430"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5764007.1"
                     /translation="MLIILSIVGVFLASCAEEGAGESAEATAETPKQPGQVAAAKTQT
                     PAAVDGGDSGLLPHQKLARDLLQELVEIDTTDSNGDNSAAAEAMAARLLAAGFPEEDV
                     KVLKPRDKKGNLVARYRGRNVDRKPLLLLAHIDVVEADPADWTLDPFTFIEQDGYYYG
                     RGTTDDKDEAAIHVANLIRLRQEGFQPDRDIIIALTADEESGTANGVQWLLENHRDLI
                     DAEYALNEGGGGAMQDGRRISNAVQASEKVYQSYLLEVTNPGGHSSLPVKDNAIYRLA
                     DALARIRDYDFPVSLNDVTRVYFERSAAIEGGELGEAMRGILESPPDPGAIAFLSGKP
                     FYNSRLRTTCVATMLSGGHAENALPQRARANVNCRILPGEPVENVQAALETAIADNEV
                     LVSPVAEAKPSPPSPLTPEVLGPIEQITEDLWPGVPVLPTMSTGATDGLYLRNAGIPV
                     YGVSGLFSDIDDNRAHGQNERILIRSYFEGQEFLYQLTKALSRADTQ"
     gene            3745..>4468
                     /locus_tag="VFY27_00435"
     CDS             3745..>4468
                     /locus_tag="VFY27_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5764008.1"
                     /translation="MTEAEASRTGVGVLRYAGRAIELVWSTSRVLTVAFGLLTLVAGV
                     LPAGIAWLGQLIVDSVVAAINDQAPLRPTLMFIAAEALLVAVVAAAQRGIKMCQSLLR
                     AQLGHRVNVLILEKAQELAMAQFEDAEFYDKLTRARREASSRPLSLVNRTFALGQNAV
                     TLVSYAVLLSQFSVWAVLILLIAGFPSFFAETKFAGDAFRLFRWRSPESRKQMYLETV
                     LAREDHAKEVKLFRIAPIFLQRY"
BASE COUNT          829 a         1381 c         1365 g          893 t
ORIGIN      
        1 cataaccctc gacgaccgga gcgatgttcc gcaggcctcc cgacacgcgc aggtcgacgt
       61 acagcaactc gtctatgccg gccgggcccc cgattacctg gtgcgtcgct tcaccgttga
      121 ctgcatccat ggaactgaga aaagcccggc gagcactttc gttggcaaga tccactgcca
      181 ccatcacggc tactccgacg cagatgccga cgagcgccag cgccagttgc cagggatgcc
      241 tcgtgagata ccctaagctg gctcgataca ggaccggcat ccgaattgtc ttcagtgcgc
      301 gttggtgggg gcaaggaggc cttcgcgcaa ctcgaaccgg cgatcgcaga aggcggcgac
      361 cacggcactg tgagtagcta tgatcatcgt gccgccttgc gcccgcacca ggttgtcgag
      421 caagctcaag acatcggcgg cggtaacatc atcgagattg ccggtgggtt cgtccgcaag
      481 taacagcgac ggcttgtgcg ccagcgcacg tgctatcgcg acccgctgct gttcgccgcc
      541 cgacagcacg tccggataag ttgcgtgtcg atctccgagc ccgacggcgg cgagcaggtc
      601 cgagacgcgc tggttcgcct cagtgcccgg aacatcgttc aactcgagga ccaggcgcac
      661 gttatcggct gcagtgagtg tcggcaccag gttgaacgcc tggtagacga agcctatgtt
      721 cctgcgccga taaagcgtgc gctcgcgttc ggacatcgcc gtaatggtcg caccgtcgat
      781 ctgtatctct ccttcatccg gctcatcgat gccggaaatg agattaagga gcgtcgattt
      841 cccggagcca cttcggccgc gcagcgcgat ggtttcacca tgtgagaagt cggcattgag
      901 gcttgccagc acgacgtgtc gcctggtccc ttcctggtaa cttcgggaca ggttgcggat
      961 gcgaatgtgg gcagttgcat ttgcgtcgtt catcgctgca tagtctacag aagggtttgc
     1021 agaggcgccg ccgcgtgatc ggaacagcaa ttgaatgatt gacgctctga tgctggtggc
     1081 ggggctcgcc ctgctgctca agggcgcgga tttcctcgtt gcaggcgcgt ccgaggtggc
     1141 ggaacggctg ggtatctccc atctcgtgat cgggctgacc gtggtggcat tcggcacttc
     1201 gatgcccgaa gtcgtggtca cccttatctc cggactcggg ggtcatcccg accttgcgat
     1261 cggcaatatc ctcggcagca acatcgcaaa catccttctc gttctcggca tagcggccat
     1321 catccggccg ctgcccgtca atgactcgac ggtggtgtcc gaaattccgt tctccctgac
     1381 ggctgccctc ctggtcgggt tcctggcgaa cgccgcgctg ttctcctcca agccagagct
     1441 cagcatcagt cacgttgacg gggcaatcct gctgttcttc tttttcctgt tcggcatcta
     1501 tatctacaac attacgcgca agcaggctgt tcccttcgaa gaacgagccg agcgtaagcc
     1561 gttgctgaag ccggttacga tgttgctggt tggcgttggc gcgctgtggc tcggcgggga
     1621 ctgggtcgtg ggaagcgcca tcagcattgc gcgcaacctg gggatcagcg attcattgat
     1681 cgggctcacg atcgtcgcag tgggaacatc tttgccggaa ctcatggcct ccgccgttgc
     1741 ggcctatcgc aaccagccgg acatcgccgt cggcaacgtc gtcggctcga acattttcaa
     1801 cctgctctgg gtgctcggaa tagcttcgct gatcacggag ctgccgttcg aagtcgtcag
     1861 caatgccgac ctcgtgatgg tgatcgtcgc cagcacgctc cttctgcttg cgctggtaag
     1921 cagtcgcgca acctgcgtat tgcgcgtgca tggcgtcatc ttcgtcctga cgtacggcgc
     1981 gtatctcacg tacctgatcc gccgtggttg aggctgctca gattcgcggg gcgatcaact
     2041 gtccctgcac cggctcgcac agcacttcgt ctctcgcgcc tatccaaacc gcgttcgatc
     2101 gttgataatc gccgccgtta ctggagggat cagatggtac taacgcaagt acgaatgctc
     2161 attatcctgt cgattgtcgg tgtcttcctc gcatcctgcg ccgaggaagg tgccggggaa
     2221 tcggccgaag cgacggcgga aacacccaag cagccaggac aggtggcagc ggcgaaaacg
     2281 caaacgcctg ccgccgtcga cggcggcgat tccggtcttt tgccgcacca gaaactggcc
     2341 cgcgatcttc tgcaagagct ggtggaaatc gacaccaccg acagcaacgg cgacaactcc
     2401 gctgcggcgg aggcaatggc agcccgcttg ctggcggcgg gatttccgga agaggatgta
     2461 aaggtcctta agccccggga caaaaagggg aacctggtgg cacgctaccg cggccgcaac
     2521 gtcgatcgca agccgttact gctgcttgcg catatcgatg tggtcgaggc cgatccggcc
     2581 gactggacgc tcgacccttt caccttcatc gaacaggatg gctactacta cggacgcggc
     2641 acgacggatg acaaggacga ggcagcgatc cacgtcgcga acctgattcg cctgagacag
     2701 gaggggttcc agcccgaccg cgacatcatc atcgccctga ccgccgatga agagagtggt
     2761 acggcgaacg gcgtacagtg gcttctggag aaccaccgtg atctgatcga cgcggagtac
     2821 gcgctgaatg aaggtggcgg cggcgcaatg caggacggcc gtcggatctc gaacgcggtc
     2881 caggccagcg agaaggttta ccagagttac ctgctggagg taaccaatcc cggcggacac
     2941 agttcactgc cggtgaaaga caacgcgatt taccgcctgg ccgatgcgct cgcgcggatc
     3001 cgcgactacg acttccctgt ttccctgaat gatgtaaccc gcgtgtactt cgagcgctcc
     3061 gcagcgatcg agggaggcga actcggcgaa gccatgcgcg gcatcctcga gtcgccaccc
     3121 gaccctgggg cgattgcgtt cctttccggc aagccgtttt acaactcccg cctgcgaacg
     3181 acctgcgtgg cgacgatgct ttcgggcggg cacgcggaaa atgcgcttcc tcaacgcgca
     3241 cgcgccaatg tgaattgccg aatcctgccg ggagagccgg tggagaacgt gcaagctgca
     3301 ctggagacgg cgattgccga caacgaggtg ttggtctcgc ccgtagcgga agcgaagccg
     3361 tcgccaccct cgccgctcac tccagaagta cttgggccga tcgagcagat taccgaagat
     3421 ctgtggccag gggtgccggt gctgccgacg atgagtaccg gagctaccga cgggctttac
     3481 ctgcgcaatg ccggcattcc ggtgtacgga gtgtcaggcc tcttcagcga catcgacgac
     3541 aaccgcgcgc acgggcagaa tgaacgcatt ctcatccgct catatttcga agggcaggag
     3601 ttcctgtacc agctgaccaa ggccttgtcg cgagccgata cgcagtaaac cggaccactc
     3661 atgatcgacc tcgcaccgct ggatatgatc ctgccccatt gtcgcgccga ttcatgaaca
     3721 gtccgggtta aggatccgtt ccccttgacg gaagccgaag ccagccgcac gggcgtcggc
     3781 gtcctccgat atgccggccg cgcgatcgaa ctggtgtggt ccaccagccg cgtgttgacc
     3841 gtggccttcg gcctgctcac gttggttgcc ggcgtgctgc cggccggcat cgcgtggctc
     3901 ggccagctga tcgtcgattc tgtcgttgcg gccatcaacg accaggcgcc cttgcgtccc
     3961 acgctcatgt tcatcgccgc ggaagctctt ctcgtggccg tcgttgccgc ggcgcagcga
     4021 ggtataaaga tgtgccagtc gctgttgcgc gctcagctcg ggcaccgcgt caatgtgctg
     4081 atcctcgaga aggcgcagga actggcgatg gcacagttcg aggacgcaga gttttacgac
     4141 aagctgacgc gggcgcggcg cgaggcttcc agccggccac taagcctcgt caaccggacc
     4201 tttgcgctcg gtcagaatgc agttacgctc gtcagctacg ctgtgttgct gtcccagttt
     4261 tcggtctggg cagtgctgat cctccttatc gcgggcttcc cttccttctt cgctgaaacg
     4321 aaattcgctg gcgacgcgtt ccggctgttc cgctggcgtt cacccgagtc gcgcaaacag
     4381 atgtacctgg aaacggttct tgcgcgagag gatcacgcga aggaagtgaa gctgttccgc
     4441 atcgcgccga ttttcctgca gcggtatc
//
LOCUS       DASUOM010000023         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1199403,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000023 DASUOM010000000
VERSION     DASUOM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199403"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..949
                     /locus_tag="VFY27_00440"
     CDS             161..949
                     /locus_tag="VFY27_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5764009.1"
                     /translation="MTVRIVLVGTTHPGNIGAVARAMKNMGLSELRLVRPRYFPHEDA
                     TARASGAEDVLQAAQVADSLGAAVADCHFVVGASARPRTIGWPTLMPRECAARLTRES
                     RSGHVAAVFGPEKAGLTNEDLDLCHALVTIPTSPDFSSLNLAMAVQVLTYELRLAAPD
                     GAVGEIPRDTPLASAAELERFYVHLEQVLTASGFLDAANPRYLMRRLRRLFLRAEPDR
                     NEINILRGILASLDPHRVQHAAAAQPAAEDERRQDAGQDRGVHK"
     gene            946..2142
                     /locus_tag="VFY27_00445"
     CDS             946..2142
                     /locus_tag="VFY27_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5764010.1"
                     /translation="MTLRKEFIYLDDAATTTVDPAVAEAMQRCLRGGGAFANPASLHV
                     AGRASHALVERSRAQVAGLLNTAPRNLIFTSGATESDNLAITGAAQFRAHRGRHLVSM
                     RTEHKAVTDCFASLEREGFQVTWLKPDRDGLLPIADLEAAIRPDTQLVSIMHVNNETG
                     VIQDIHQIGECCRRHGVLFHCDAAQSAGKLPIDLSRLPVDLMSLTAHKFYGPQGIGAL
                     YLADRPECHIRPLLHGGGQERRLRPGTLAVHQVAGFGVAAAIATECQANDLAHVERLN
                     ARLWKGIRDVHGITRNGDPGHAFPGILNVSIDGIDGESLMLTLEPLCVATGSACNSQS
                     GESSYVLRALGLPDQLAQSAIRFSFGRFTSESDVDAAIELYTAGVSHLRNIMPETTAS
                     TALRER"
     gene            2132..2515
                     /locus_tag="VFY27_00450"
     CDS             2132..2515
                     /locus_tag="VFY27_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5764011.1"
                     /translation="MSAEPYNHTVRRHFANPTHAGDLPEGYARVVRADVSESEAGLRV
                     LLAAQIDGNIISRLRYRVFGCPHLIAAAEEFCARLEGATVEGLQDVPVTELMELLEIP
                     VEKTGRLLLLEDAANALHQACSRHR"
     gene            2676..2999
                     /gene="iscA"
                     /locus_tag="VFY27_00455"
     CDS             2676..2999
                     /gene="iscA"
                     /locus_tag="VFY27_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEX5764012.1"
                     /translation="MAISLTKPAADRVRNYLDARGGGVGLRLGVKKTGCNGFAYVINY
                     ADSIEDSDVVFEQRGIKVIVDPKSLELIDGTEVDFVKEGLNEAFRFRNPNVTGECGCG
                     ESFSV"
     gene            3200..3628
                     /gene="ndk"
                     /locus_tag="VFY27_00460"
     CDS             3200..3628
                     /gene="ndk"
                     /locus_tag="VFY27_00460"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5764013.1"
                     /translation="MSVEQTLSIIKPDGVQKNLIGEIYRRFEKAGLSIVAARMMHLSK
                     DEAQNFYAVHRERPFFNDLVRYMTSGPVMVQVLEGESAIEKNRKIMGATNPADAEAGT
                     IRADFADSIEENVVHGSDGADTARQEIRFFFGDGVCPRTR"
     gene            3643..4746
                     /gene="rlmN"
                     /locus_tag="VFY27_00465"
     CDS             3643..4746
                     /gene="rlmN"
                     /locus_tag="VFY27_00465"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5764014.1"
                     /translation="MNETQVINLLGLPQARLESFFAGLGEKPFRARQVMQWMHQRGAE
                     SFDVMSDLSKELRSRLREVAEIRLPAVISEQHSEDGTVKWLFGSGAGQAVESVFIPEP
                     GRGTLCISSQVGCALDCAFCATGAQGFNRNLTPAEIVGQVAHANRNLPRRPTGEAAVT
                     NVVFMGMGEPLANYRNLLPALDLLIADLAYGLSRRRVTVSTSGIVPHIERLGDDCNVS
                     LAVSLHAPDNDLRDRLVPINRVHPIEELLDACWRYAAKRANRFITFEYVMLNDVNDSP
                     ACADRLSKLLLNHPAKVNLIPFNPFPGSEFRRSSADAIQRFQGRLRKQGVIATTRRTR
                     GGDIDAACGQLAGKVSDRVRVRLGEKNKRIVTV"
     gene            4743..5519
                     /gene="pilW"
                     /locus_tag="VFY27_00470"
     CDS             4743..5519
                     /gene="pilW"
                     /locus_tag="VFY27_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX5764015.1"
                     /translation="MIKSRKLLAKLPLAVASLCVLLAGCVSTTTGVRPTESDDDNAAQ
                     QYFQLGARYFRNGNYELARERLHRAIDLDPRMGMAHSTLALTYEQLDNVRLATEHHDK
                     AVRYEPGNFNVRNAYAVFLCGQKRYDEAKIQFQRAVEVPENDNSEIMLTNAGVCMSQK
                     PDYDQAEAFLRQALDEKPGYGEALLQMSLLKYRTNEFLQARAFLQRFLSSNKSTPDVL
                     YLGVQIEKALGDDRASTDYANQLLREFPNSSQAKSVLRAG"
BASE COUNT         1122 a         1749 c         1778 g          990 t
ORIGIN      
        1 tgattccggc agtgattttg aaagaacccg ccgcgaccag cagtgctcat gccgggtctc
       61 ggcgaatggg cggtagaata ccagacccgc gtagcggtaa cgccccaggg tttacaacgc
      121 gcggcacatc gaacaggcaa ttgatgagta gtagcctcag gtgacagtca gaatcgtact
      181 cgtcgggacg acccatcccg gaaacatagg cgcggttgcc cgcgcaatga agaatatggg
      241 cctgtcggag ttgcggctgg tcaggccgcg gtatttcccg cacgaagatg cgaccgcgcg
      301 cgcgtcaggc gcggaagacg tcctgcaggc ggcacaggtg gcggacagcc ttggcgcggc
      361 ggttgcggac tgtcacttcg tggttggcgc cagcgcccgc ccgaggacca taggctggcc
      421 gaccctgatg ccgcgcgagt gcgcggcgag gctgacccgg gaaagcagga gcggccacgt
      481 ggcagcggtg ttcggcccgg aaaaagcggg actcacgaac gaggacctcg acctgtgcca
      541 tgccctcgtc accataccca catcaccgga tttcagctcg ctcaatctcg cgatggcggt
      601 gcaagtgctc acctacgaac tgcgactggc ggcaccggat ggcgcggtcg gtgagatccc
      661 tcgcgatacg cctctcgcca gcgccgcgga actcgagcga ttctacgttc acctggaaca
      721 ggtgctgacg gccagcggct tcctcgacgc cgccaatccg cgttacctca tgcggcgcct
      781 ccgccggctt ttcctgcgcg cggaacccga tcgcaacgag atcaacatcc tgcgcggcat
      841 actcgcgtcc ctggacccgc accgggtgca gcacgcggca gcggcgcaac ctgcggcgga
      901 agatgagcgc cgtcaggatg ccggccagga tcgaggcgtc cacaagtgac gctccggaag
      961 gagttcatct atctcgatga cgcagcgacc accacggtgg atccagctgt cgccgaggcg
     1021 atgcagcgct gccttcgcgg cggcggcgct ttcgcgaacc cggcgtccct gcatgttgcc
     1081 ggccgcgcat cgcacgcact ggtcgaacga tcgcgcgcgc aggtggcagg gcttctcaat
     1141 accgcacccc gaaacctgat cttcacctcc ggtgctaccg agtctgacaa cctggccatc
     1201 acgggcgctg cgcagtttcg cgcccaccgc ggccggcacc tggttagcat gcggaccgaa
     1261 cacaaggccg tcaccgactg cttcgcctcg ctcgagcgcg agggtttcca ggtgacatgg
     1321 ctcaagccgg accgcgacgg tctcctgccg attgccgatc tcgaggcggc aatccgcccc
     1381 gacacccagc tcgtgtcgat catgcacgtc aacaacgaga cgggcgtaat tcaggacatc
     1441 catcaaatcg gcgagtgctg ccgccgccac ggcgttctat tccactgcga tgccgcgcag
     1501 tcggccggca agctgccgat cgacctgtct cggttgcccg tggacctcat gtcattgacc
     1561 gcgcacaagt tttacggtcc gcaaggcatc ggcgccctgt acctggccga caggccggaa
     1621 tgccacatcc ggccgctgtt acatggcggc gggcaggaac gccgcctgcg gcccggcacc
     1681 ctggcagtgc accaggtggc cggtttcggt gtggccgcgg cgatcgccac ggagtgccag
     1741 gccaacgatc tcgcgcacgt ggagcgcctg aacgcgcgcc tgtggaaggg tatccgggac
     1801 gtgcacggca tcacccgcaa cggcgatccg ggccacgcgt ttcccgggat ccttaacgtc
     1861 agcatcgacg gaatagacgg agaaagcctg atgctgacac tcgagccgct ctgtgtggcg
     1921 accggttctg cgtgcaattc gcagagtggc gagtcttcct atgtgctccg ggcgctcggg
     1981 ctccccgacc agctggcaca aagcgctatt cgcttcagtt ttggccgctt taccagcgaa
     2041 tctgacgtcg acgccgcgat cgagctttac acagcggggg tatctcacct gcgcaacatc
     2101 atgccggaaa cgacagcctc gacggcactt cgtgagcgct gaaccctaca accacaccgt
     2161 gcggcggcat tttgcgaacc caacccatgc gggcgatctg ccggagggct atgctcgcgt
     2221 cgttcgcgcg gacgtttccg aatcggaagc aggtctccgg gtactgctgg cggcacaaat
     2281 cgacggtaac attatttccc ggctgcgcta ccgggtgttt ggctgccctc acctgattgc
     2341 ggcagcggaa gagttctgcg cgcgactcga aggcgcgaca gtagagggct tgcaggacgt
     2401 ccccgtgacg gaactcatgg agttactgga gattccggtc gagaaaaccg gccgcctcct
     2461 cctgctggaa gacgcggcga atgctttgca ccaggcgtgc agcaggcacc ggtaggagcc
     2521 cgccacgccg gctggtttga cggcactcgc cggcattcgc cgctcggaag gtattcgaac
     2581 ttgacacggt tgacgatttc ccgcgttggc ccctgcaggg ggaaccatcc ggcggaagag
     2641 gctatcgaag gaacgggaag gaattgaaga acatcatggc aatatcgctt acaaaaccgg
     2701 cagcggacag ggtccgaaac tacctggacg cacgcggcgg gggcgtcggc ctgcgcctgg
     2761 gagtgaagaa aacaggctgc aacggcttcg cttacgtcat aaattacgcc gacagcatag
     2821 aagacagtga tgtcgttttc gagcagcgag gcatcaaggt catcgtcgac ccgaagagtc
     2881 tcgaactgat cgacggcacg gaagtcgact tcgtgaaaga gggcctgaac gaggcgtttc
     2941 gctttcgcaa tccgaatgtc accggggaat gcggctgcgg cgagagtttc agcgtctgag
     3001 tcgcggaccg gagcatcccg gtcgcgcgct cctcacgaat cctctaagta cctgcccatg
     3061 agcggcggca ttagcgagag accggccgca cctgcggctg attgcccgcc cagaaatttg
     3121 caagtaaaat tgagggctta cgcacggccc gcgcttttga cgggctggtc caatccgaat
     3181 gaatcacaag gaacaccgta tgtccgttga gcagacgctc tccataatca aacccgatgg
     3241 tgtgcagaag aacctgatcg gcgaaatcta caggcgcttc gagaaggcag ggctgtcgat
     3301 cgtcgccgcg cgcatgatgc acctgtccaa ggatgaggcg cagaactttt acgcagtgca
     3361 ccgggagcgg ccgtttttca acgaccttgt gcggtacatg acctcgggtc cggtgatggt
     3421 gcaggtgctg gaaggcgaat ccgccatcga gaagaaccgg aagatcatgg gcgccacgaa
     3481 tcctgctgat gctgaagccg ggactatccg tgccgacttc gcggacagca tcgaggagaa
     3541 cgtcgtccac ggttccgacg gcgccgatac ggcgcgccag gaaatccgtt ttttcttcgg
     3601 cgacggggtg tgcccgagga cgcgttagcg tattcctgaa agatgaatga aacgcaggtg
     3661 atcaatctgc tcggcttgcc gcaggcgcgg ctcgaatcgt tttttgccgg gttaggcgag
     3721 aaacctttcc gcgcgcgcca ggtaatgcaa tggatgcacc agcgcggcgc ggaaagcttc
     3781 gacgtgatga gcgacctgtc gaaggaactg cgttcccgcc tcagggaggt tgcagaaatt
     3841 cgcctgcctg ccgtcatttc cgaacagcac tccgaagacg gcacggtcaa atggctgttc
     3901 ggttccggag ccggccaggc tgtcgagtcg gtgttcattc cagagccggg caggggcacg
     3961 ctgtgcatat cttcgcaggt cggctgcgcc ctcgattgcg cgttttgcgc caccggcgcg
     4021 cagggcttca accgcaacct gacgccggcg gagatcgttg gccaggttgc ccacgcgaat
     4081 cgcaacctgc cgcgccggcc caccggcgaa gccgcggtga cgaacgtcgt gttcatgggc
     4141 atgggcgagc cactcgcgaa ctatcgcaac ctgctgccgg ctctggacct cctgattgcc
     4201 gacctggcgt acggcctctc tcggcgacgc gtaacggtca gcacttccgg aatcgttcct
     4261 cacatcgagc gcctgggcga cgactgtaat gtgtcgctgg ccgtatcgct gcacgcgccg
     4321 gacaacgatc tcagggatcg gctggtgccg atcaaccgcg tgcatccgat cgaggaactg
     4381 ctggatgcct gttggcgcta tgcggccaag agggcgaacc gcttcatcac gttcgaatac
     4441 gtaatgctga acgacgtcaa cgactcgcct gcctgcgcag atcgcctctc aaagttgttg
     4501 ctgaaccacc ctgcgaaagt gaacctgata cctttcaatc ctttcccggg cagtgagttc
     4561 cgccgttcct ctgcggacgc catccagcgt ttccagggcc ggctccggaa gcagggcgtc
     4621 attgcaacca cccgcaggac gcgtggcggc gatatagatg cagcctgcgg acaattggct
     4681 ggtaaagtga gcgaccgggt gcgggtcagg ctgggcgaga agaataagag gatcgtaacg
     4741 gtatgataaa aagcaggaaa ttgcttgcga agctgccgct cgcagtggcc tcgctgtgcg
     4801 tgttgctggc tggctgcgta tccacgacca ccggggtcag gccgactgag tcggatgacg
     4861 ataatgcggc gcaacagtat ttccagctcg gtgcccgcta cttccgcaac ggcaactacg
     4921 agctggccag ggagcgactg catcgcgcca tagatctcga cccgcggatg ggtatggccc
     4981 actcgacgct cgcgctcact tatgagcagc tggataacgt ccggctcgcc accgaacacc
     5041 acgacaaggc cgtgcgctac gagcccggca acttcaatgt gcgcaacgcc tacgccgtgt
     5101 tcctttgcgg ccagaagcgt tacgacgagg caaaaataca attccagcgc gcggtggaag
     5161 tgccggagaa cgacaacagc gaaatcatgt tgaccaacgc cggcgtctgc atgtcgcaaa
     5221 agccggacta cgaccaggcc gaagctttcc tgcggcaggc gctcgacgag aagccgggtt
     5281 atggcgaggc cctgctgcag atgtccctgt tgaagtacag gacgaacgag ttcctgcagg
     5341 cgcgtgcatt cctgcagcgt ttcctgtcct ccaacaagag cactccggac gtgctatacc
     5401 tcggggtgca gatagagaaa gcactgggcg acgaccgtgc aagcaccgac tatgcgaacc
     5461 agctgttgcg tgagttcccg aattcaagcc aggcgaaaag cgtattgcgg gcgggctagg
     5521 gcggcagtat gagcgatgaa gctacaacgc cgccggacgg tggagacagc ggccggctcg
     5581 ccggcgaacg cctcgcggat gcccgtgccg gacgcgatat ccccattcac gagattgcg
//
LOCUS       DASUOM010000024         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_111594,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000024 DASUOM010000000
VERSION     DASUOM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111594"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..166)
                     /locus_tag="VFY27_00475"
     tRNA            complement(77..166)
                     /locus_tag="VFY27_00475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Ser,seq:cga)
     gene            384..638
                     /locus_tag="VFY27_00480"
     CDS             384..638
                     /locus_tag="VFY27_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764016.1"
                     /translation="MSRFIHWLRKRDTGQATFRIGDRVRITSADSPFADRVGVEAIIV
                     SGLERAGDGRSYYRLDNNMSAQPECLTLLEPGVDAASIAD"
     gene            complement(656..835)
                     /locus_tag="VFY27_00485"
     CDS             complement(656..835)
                     /locus_tag="VFY27_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764017.1"
                     /translation="MTKDPKEINEARAKKREDTQHQYREAVALEQIADTLESMRLEFI
                     DFRKTVIRALESRER"
     gene            complement(889..1350)
                     /locus_tag="VFY27_00490"
     CDS             complement(889..1350)
                     /locus_tag="VFY27_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5764018.1"
                     /translation="MSKSLAVIVAAVMLVGCAPSWAQADVRQEVFAAERAFAQTMADR
                     DHETFASFIAEEAIFFGPAEPLRGKQQVVEGWAPFFEGADAPFSWEPQTVEVLASGTL
                     ALSSGPVRNKAGETVAQFTSIWRRESDGAWRVVFDKGCDLCAREAAGKPGG"
     gene            complement(1418..2323)
                     /gene="rimK"
                     /locus_tag="VFY27_00495"
     CDS             complement(1418..2323)
                     /gene="rimK"
                     /locus_tag="VFY27_00495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX5764019.1"
                     /translation="MKIGILSRNRRLYSTKRLVEAASERGHEVRVINHLRCYMNIASF
                     KPSIHYNGELLPQLDAIIPRIGASVTFYGSAVVRQFEMMGTFSLNESVAISRSRDKLR
                     SLQLLSRKGIGLPVTGFANSPDDTYDLLQLVGGAPVVVKLLEGTQGKGVVLAETRKAA
                     ESVVEAFRGLNANFLVQEFIKEAGGADIRCFVIGDKVVASMKRQAQEGEFRSNLHRGG
                     SSKLIKITPEERSTAVRSARIMGLNVAGVDILRSNHGPVVIEVNSSPGLEGIETATGR
                     DIAGMIIAYIEKHWKTGKTRTKGAG"
     gene            complement(2320..2787)
                     /locus_tag="VFY27_00500"
     CDS             complement(2320..2787)
                     /locus_tag="VFY27_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEX5764020.1"
                     /translation="METNNRLVTVGWREWVGLPELGIARIKAKVDTGARTSALHAFHV
                     ERFEKDGRDWVRFKIHPRQYRTDSEIICEAPIRDQRMVSDSGGHRESRYVIETLLTLP
                     GLSWPIEITLTSRDDMRFRMLLGRSALVNRMIVDPSRSYLTRRRKRNRKREAK"
     gene            complement(2790..3815)
                     /locus_tag="VFY27_00505"
     CDS             complement(2790..3815)
                     /locus_tag="VFY27_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5764021.1"
                     /translation="MPGQTVVVGKQEISPGTRKTVELDVANLYTHAHLTMPVHVINGR
                     ADGPTLFLTAAIHGDELNGVEIIKRVMKRAVLKSLSGCILAVPVVNIFGFIDQSRYLP
                     DRRDLNRSFPGRVTGSLAARLAHMVATEIVANSNFGIDLHTGPVHRTNFPQIRVNLSD
                     PDSRRMANAFGAPVVIDANVREGTLRAYAGSQGVPTLLYESGEALRLDELSIEAGVNG
                     VIRVLRELEMLPAHSKTAKVVEPTYANDTSWVRAPMSGIINMRCRLGQRVKSQQVLAK
                     ISDPFGEIETDVVAPAGGIVIGRSTSPLTHEGDALFNLARFDDTREAKATVDEFQEAS
                     AIEHNWG"
     gene            4015..4464
                     /locus_tag="VFY27_00510"
     CDS             4015..4464
                     /locus_tag="VFY27_00510"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5764022.1"
                     /translation="MAGLYFEEFQVGQTFRHSIRRTITEADNVWFSALTHNPAPIHID
                     AEYCRQTEFGKPLVNSCLTLGFMVGISVGDTTAGTTVANLGWDEVRFPKPLFHGDTIH
                     VETEVLELRDSKSRPNNGIVVFTHRAFNQHDELVGHCRRTALMLRRP"
     gene            4465..5352
                     /locus_tag="VFY27_00515"
     CDS             4465..5352
                     /locus_tag="VFY27_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5764023.1"
                     /translation="MTLVNIRSFLFVPADSSKKLLKAADSDADALIVDLEDAVAPREK
                     PRARSIAREFVSEYRQRGCWVRINPLDTEDALPDLRALMSSAPAGIVLPKPYGANDAI
                     QLGKLLDVLEQESALPPGSTAILPVATERPGALFRLHEYARSTERLAGLTWGAEDLGA
                     ALGASANRDGQGNWLPPYELARSLCLFAAAAAAVPAIDTVFTDYRDHDGLRASADRAR
                     RDGFSGMLAIHPAQVAIINEAFLPSSAEIERARRVVELFAATPDAGTVGLDGRMLDRP
                     HLLQAQRILEAARRLKLET"
BASE COUNT         1057 a         1708 c         1659 g         1105 t
ORIGIN      
        1 ttttgttggt cgatatgttg ggcgcaaaat gaaaaatacg ccaatacctt gatatttcgg
       61 cgtgattttc agtaattggc ggagagggtg ggattcgaac ccacggtacg gttgcccgta
      121 ctcttgattt cgagtcaagc ccattcgacc actctggcac ctctcctctt ctcccgtcga
      181 aacgggggcg caagtctacc ggctcggcgc tgttccttcc atgaaaacca accctgcccg
      241 gaaaatcgcg ctatgcctca cgacactgcc aatgtggacg cgataaactc gagggtatgg
      301 ccaaaagaag gcgaacgacg accaggtcat gggcggaggg ggccgaggcg gttagaaccg
      361 ccccgcgaaa acctgagtcc gtggtgagcc gctttatcca ctggttgcgc aaaagagata
      421 ccggacaggc gaccttccga atcggcgatc gcgtccggat caccagcgcg gattcgccat
      481 tcgccgatcg tgtcggcgtc gaagcgatca tcgtgtccgg actggagcgt gcgggtgacg
      541 gccgcagcta ttaccgtctc gacaacaaca tgtcggcaca accggaatgc ctgaccttgc
      601 tcgagccggg tgtcgatgcc gcgagcatcg cggattaggc tccgcagcgt tgctctcagc
      661 gctcccggct ttcaagagcg cgaattaccg tcttcctgaa atcgatgaat tcgagccgca
      721 tcgattcgag cgtgtcggca atctgctcca gcgccaccgc ctcacgatac tggtgctggg
      781 tgtcttcacg tttcttggct cgtgcttcgt tgatttcttt cggatctttg gtcatggtgg
      841 ctccggtgtt cgggcgtaag aatgcggcgc aggcgctacg cgcagcggtc agccgccggg
      901 ttttcccgcg gcttcccgcg cgcaaaggtc gcagcccttg tcgaagacga cacgccaggc
      961 gccgtcggac tcgcgccgcc agatcgaggt gaactgcgcg actgtctccc cggccttgtt
     1021 tcgcactggt cccgaactca gcgccagcgt gcccgaagca aggacctcga cagtctgcgg
     1081 ttcccaggaa aaaggcgcat cggcaccttc gaaaaagggc gcccatccct cgacgacctg
     1141 ttgcttgccc cgcaggggtt cggcagggcc gaaaaatatt gcttcttccg caatgaaaga
     1201 cgcaaaagtt tcatggtcgc gatcggccat ggtttgcgcg aaggcgcgct ctgctgcgaa
     1261 cacttcctga cgaacgtccg cttgcgccca cgaaggcgcg cagccgacca gcatgactgc
     1321 agcgacgata acggcaaggc tctttgacat cgagtgacct ccgcgacagt tatagacctt
     1381 atccgctacc gattacctgc catcggtgac gggtacacta gccggcaccc ttggtccggg
     1441 tcttgccggt cttccagtgt ttttcgatat acgcgatgat catcccggcg atatccctgc
     1501 cggtcgcggt ttcgatgcct tccaggcccg gggaggaatt gacctctatg acgacgggac
     1561 cgtggtttga ccgcaggata tccacaccgg cgacgttgag acccatgatc ctggccgagc
     1621 gaaccgcggt cgaccgctcc tctggcgtga tcttgatcag cttcgatgac ccgccgcgat
     1681 gcaggttgga ccgaaactcg ccttcctgcg cctgccgttt catcgaagcc accaccttgt
     1741 cgccgatgac gaagcagcga atgtccgcgc caccggcttc cttgatgaat tcctgcacga
     1801 ggaagttggc attcaaccca cgaaaagcct cgacgacact ctcggccgcc ttgcgcgttt
     1861 cggcaaggac gactcccttc ccttgcgttc cttcgagcag ctttacgacc accggcgctc
     1921 cgccgacgag ctgcagcagg tcatacgtat cgtccggcga attggcaaag ccggtgaccg
     1981 gcagtccgat gccctttcgc gaaagaagct gcaaagacct cagcttgtcc cgcgaacgcg
     2041 agatggcgac agattcgttc agcgagaacg ttcccatcat ctcgaactgg cgcaccactg
     2101 cagaaccgta gaacgtaacg gatgctccga tacgcgggat gattgcatcg agctgcggca
     2161 gcagttcgcc gttgtaatga atgctgggtt tgaagctcgc gatattcatg tagcagcgca
     2221 ggtggttgat aaccctgacc tcgtgcccac gctccgacgc cgcctcaacg aggcgctttg
     2281 tcgagtacag tctcctgttc ctggaaagaa taccgatctt catttagctt cccgtttacg
     2341 gttgcgtttg cggcgcctgg tcagatagga acgggacggg tcgacgatca tgcgattcac
     2401 gagcgccgac cttcccagca acatgcggaa gcgcatgtcg tctctcgaag tgagcgtgat
     2461 ttctatcggc catgataacc caggcaaggt caggagtgtt tcgatgacgt accggctctc
     2521 cctgtggccg ccggagtcgg acaccattcg ctggtcccgg ataggggcct cgcagatgat
     2581 ctcggagtcg gtccggtatt gtcgcggatg gatcttgaag cgcacccagt cgcgaccgtc
     2641 cttctcgaaa cgttcgacgt gaaacgcgtg cagcgctgat gtccttgcgc cggtgtcgac
     2701 cttcgccttg atgcgagcga tgccgagttc cggcaggccc acccattcac gccagccgac
     2761 agtcaccagc ctgttgttgg tttccaagac taaccccagt tgtgctcgat cgcgctcgct
     2821 tcctggaact cgtccacggt cgccttggcc tcacgcgtgt cgtcgaatcg cgcgaggttg
     2881 aacaacgcat ctccttcgtg agtgagcggg gaagtgctgc gcccgataac gatgccaccc
     2941 gccggggcaa cgacgtcggt ttcgatctcc ccgaaaggat cgctgatctt cgccagcacc
     3001 tgctgcgatt tcacgcgctg cccgaggcgg cagcgcatgt tgatgatgcc gctcatcggg
     3061 gcgcgcaccc aactggtatc gttggcgtaa gtgggttcga ctaccttcgc tgtctttgaa
     3121 tgcgccggca gcatctccag ttcgcgaagc acacggatga ctccgttcac gccggcctcg
     3181 atcgagagct catccagccg cagcgcctcg ccgctttcat agagcagcgt cggtacgccc
     3241 tgcgagccgg cgtaagccct gagcgtgccc tcgcggacgt ttgcatcgat gacgaccggc
     3301 gcgccgaacg cattggccat ccggcgggag tcgggatcag agaggttcac gcgaatctgc
     3361 gggaagttcg tccgatgcac cggcccggta tgcaggtcta tgccgaagtt cgagttcgcg
     3421 acaatttcag tggccaccat gtgcgcgaga cgcgccgcaa gcgagccggt cacccggccg
     3481 gggaacgagc gattcaggtc gcgccggtcc ggcaggtaac gcgactggtc gatgaagccg
     3541 aagatattaa ccaccggcac ggccaggatg cagccactca aactcttcaa aaccgcccgt
     3601 ttcatcactc gcttgatgat ttccacgccg ttcagttcgt ccccgtgtat ggcggccgtg
     3661 agaaacagcg tcggcccgtc ggcgcgtccg ttgatgacat gcaccggcat cgtcaggtga
     3721 gcgtgggtgt agagattggc aacgtccagt tcgacggttt tgcgggttcc ggggcttatt
     3781 tcctgcttgc cgacgacgac ggtctgtcct ggcattcaat actcctgtaa tcgcatccgg
     3841 cggtagcggg catggccggg cgcggctagt gtaccccgtc ataatcaatg gaccattggc
     3901 gcgggcgttt tcgcggcagc cggcaatcgt cgcggcagtt tacataatgc gcgtttgatt
     3961 ggactaccgc aggcattatg atgcgaggtc gttttcatct tggggccttc aggcatggcg
     4021 ggtttgtact tcgaggagtt ccaggtcggg cagacgttcc ggcattcgat tcgccgcacc
     4081 atcaccgagg ccgataacgt ctggttcagc gcgctcaccc acaatcccgc gccgatccac
     4141 atcgatgccg aatactgcag gcaaaccgag ttcggcaagc cgctggtgaa cagctgcctg
     4201 acgctcgggt tcatggtcgg gatctcggtc ggcgacacga ccgcgggaac cacggttgca
     4261 aacctcggat gggacgaggt gcgttttccc aagccgctgt tccacggcga tacgatccac
     4321 gtcgaaacag aggttctcga actgagggac agcaagtcca ggcccaataa cggcatcgtt
     4381 gttttcaccc accgcgcctt taaccagcac gacgaactgg tagggcattg caggcgcacc
     4441 gcgctgatgc tgcgcaggcc gtaagtgacg ctggtgaaca ttcgcagctt cctgttcgtc
     4501 ccggccgaca gcagcaagaa gcttctcaaa gccgcagatt ccgacgccga cgctctcatt
     4561 gtcgatctcg aggatgccgt tgcaccgcga gagaagccgc gtgcccgctc catcgcccgc
     4621 gaattcgtgt cggaataccg ccaacgtggc tgctgggttc gcatcaatcc gctggacacg
     4681 gaggatgcgc ttccggacct gcgggcgctc atgtcctcgg cacccgcggg aatcgtcctg
     4741 ccgaaaccct acggcgcgaa cgacgcgatc caactcggta aactccttga cgtcctggaa
     4801 caggaatccg ctctgccgcc ggggagcact gcgatacttc ccgtggctac cgagcgtccc
     4861 ggtgcgctgt tccgcctcca cgagtacgcg cgctcgactg agcgtctcgc gggcctcacc
     4921 tggggtgccg aagacctggg agcagcgctt ggtgcttcag cgaaccgcga cgggcaaggg
     4981 aactggctcc cgccctacga gctcgcacgg tcgctgtgcc tctttgccgc ggctgcggcg
     5041 gcggtgccgg ccatcgatac ggtatttacg gactaccgcg atcatgacgg actccgcgcc
     5101 agcgctgatc gggcaaggcg cgacggcttc agcggaatgc ttgcgattca cccagcgcag
     5161 gtcgctatca tcaacgaggc gttcctgccg agcagcgccg aaatcgaacg ggccaggcgg
     5221 gttgtcgagt tgttcgcggc aacgccggat gccggtacgg tgggccttga cggccggatg
     5281 ctcgaccggc ctcatttgct gcaggcgcag cgaattctcg aagcggccag gcgcctgaag
     5341 ttggagacgt gatgaaagaa cgtgcaagcg gagtatggca atgaccaacg tatacagagt
     5401 cagcgaagtt gccgccagga cggccggtat cgacaaggaa acctatgatg cgacctggcg
     5461 acggtcgatc accgacaacg aagcgtactg ggcagagcag gccgggtgca tcgactggac
     5521 aaggaagtt
//
LOCUS       DASUOM010000025         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_353652,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000025 DASUOM010000000
VERSION     DASUOM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353652"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFY27_00520"
     CDS             complement(<1..873)
                     /locus_tag="VFY27_00520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5764024.1"
                     /translation="MADDASSKPDLLDDRKFTINFGPQHPAAHGVLRLVLDLDGEVVD
                     RVDPHIGLLHRGTEKLIEYKTFLQALPYFDRLDYVAPMNQEHSYCLAIERLAGIEVPR
                     RAQFIRVIYSEIGRLLSHILNVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMH
                     AAYFRPGGVHQDLPPELIEDIHKFCDPFLKVVDDLETLVTENRIFKQRNVDIGVVSVE
                     DALAWGFSGVMVRGSGLAWDLRRAEPYEVYSELDFKIPVGKNGDCWDRYLCRIEEMRE
                     SIKIIKQCIDMMPVG"
     gene            complement(876..1505)
                     /locus_tag="VFY27_00525"
     CDS             complement(876..1505)
                     /locus_tag="VFY27_00525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5764025.1"
                     /translation="MSVLPTRAAMLAELKIHLEATQAKVISSMHVVHGELTVNVPRDA
                     IVKLLSFLRDDSQCRFETLVDICACDYPTETERFEVVYHMLSMRLNQRIRVKLRTDEM
                     TPVPSVTGVFPVANWYEREAFDMYGVIFSGHPDLRRILTDYGFEGYPLRKDFPLTGRV
                     EVRYDESQKRVVYEPVKLTQEYRNFDFLSPWEGMQSVIPGDEKAGGGKA"
     gene            complement(1502..1975)
                     /locus_tag="VFY27_00530"
     CDS             complement(1502..1975)
                     /locus_tag="VFY27_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5764026.1"
                     /translation="MSDELADKGFLVAAADDLITWARTGSLMWMTFGLACCAVEMMQA
                     SMPRYDMERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCA
                     NGGGYYHYSYAVVRGCDRIVPVDIYVPGCPPTAEALLYGVLQLQKKIRREGSIER"
     gene            complement(2071..2445)
                     /locus_tag="VFY27_00535"
     CDS             complement(2071..2445)
                     /locus_tag="VFY27_00535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5764027.1"
                     /translation="MEDAQALVTSYLPVVIFLVIAAVLSVLFIVLTTLFAPTNPDTEK
                     LSAYECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWVFQVAEGNVVAFWSMM
                     AFLGVLTVGFIYEWRRGALEWQ"
     gene            complement(2625..2701)
                     /locus_tag="VFY27_00540"
     tRNA            complement(2625..2701)
                     /locus_tag="VFY27_00540"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2665..2667),aa:Asp,seq:gtc)
BASE COUNT          608 a          889 c          852 g          542 t
ORIGIN      
        1 acctacaggc atcatgtcaa tgcactgctt gatgatcttg atcgactccc gcatctcctc
       61 gatgcggcag agatagcggt cccagcaatc gccgttcttg cccaccggaa tcttgaagtc
      121 gagttccgag tagacttcgt agggttctgc gcggcgcaga tcccaagcga ggcccgaacc
      181 gcgcaccatc actccggaga agccccatgc cagcgcgtcc tcgaccgaca ccactccgat
      241 gtcgacgttg cgctgcttga agatacgatt ctcggtgacc agcgtctcca gatcatccac
      301 caccttgagg aacggatcgc agaatttgtg aatgtcctcg atcagctccg gcggcaggtc
      361 ctgatgcacg ccgccgggac ggaaatacgc cgcatgcatc cgcgaacccg acgcgcgctc
      421 atagaatacc atcagcttct cgcgttcctc gaagccccac aaaggaggcg tcaacgcgcc
      481 tacgtccatc gcctgagtgg tgacgttgag gatatgcgac agcaatcgcc cgatctcgga
      541 gtagatgact cgaatgaact gggcgcgcct cggcacctcg atgccggcca gccgctcgat
      601 ggcaagacag taggagtgct cctggttcat tggcgcgaca tagtcgagcc gatcgaagta
      661 cggcagggct tgcaggaagg tcttgtactc gatcagcttc tcggtgccgc gatgaagcag
      721 tccgatatgc ggatcgacgc gatccacgac ctcgccgtcc aggtcaagca cgagcctgag
      781 gacgccatgc gccgccggat gctgaggtcc gaagttgatg gtgaacttcc gatcatccag
      841 caggtcgggc ttggaggaag catcgtcggc catgactatg ccttgcctcc gccagccttc
      901 tcatctcccg gaatcacaga ctgcatgccc tcccacggag agagaaagtc gaagttgcgg
      961 tactcctgcg tcagctttac gggctcgtag acaacccgct tttgggattc atcgtagcgt
     1021 acctctaccc ggccggtgag cgggaagtcc ttgcgcaacg gatatccttc gaatccgtag
     1081 tcggtgagga tgcggcgcaa gtcgggatgg ccggagaaga tcacgccgta catgtcgaac
     1141 gcttcgcgct cgtaccagtt ggcgaccgga aagacgccag tcacgctcgg caccggtgtc
     1201 atctcgtcgg tgcgcagctt cacccggatc cgctggttca gccgcatgga cagcatgtga
     1261 tagacgacct cgaaacgctc cgtttcagtc gggtagtcgc atgcgcagat atcgaccagc
     1321 gtttcgaacc ggcactggct gtcgtcgcgc agaaacgaca gcagcttcac gattgcgtcg
     1381 cgaggaacgt tcacggtcag ctcgccatgc acgacgtgca tcgacgaaat gactttggcc
     1441 tgcgtcgctt cgagatggat tttgagttcc gcgagcatgg cggcccgggt tggaagcacg
     1501 ctcatcgctc gatgctcccc tcgcgccgaa tttttttctg aagctgcagc acgccataga
     1561 gcagcgcctc tgccgtcggc gggcagccgg gtacatagat gtctaccggc acgatccgat
     1621 cacatccgcg aacgacggcg taggaatagt gatagtaccc gccgccattg gcgcatgacc
     1681 ccatggagat cacgtagcgc ggctccggca tctggtcgta gaccttgcgc agggccgggg
     1741 ccatcttgtt ggtcagcgtc ccggcgacga tcatcacatc gctctggcga ggcgaggccc
     1801 gcggcgcgaa tccgaagcgc tccatgtcgt agcgtggcat cgacgcctgc atcatctcga
     1861 ccgcacagca ggcgagaccg aacgtcatcc acatcagcga gccagtgcgc gcccaggtga
     1921 tgagatcgtc ggcagcagca acaaggaagc ccttgtcggc cagctcgtcg gacaggccgg
     1981 tgaagaacgg gtcgtccggc ttgacgagcg cacccccttc cggtcgcaga acgcgccctg
     2041 cagagccagc cggcgccttt aggtcagcca tcactgccac tccagggcgc cgcggcgcca
     2101 ctcatagatg aagccgacag tgagcacgcc gaggaaagcc atcatcgacc agaaagccac
     2161 gacgtttccc tcggccacct ggaagaccca cggaaacagg aacgccacct caaggtcgaa
     2221 gatgatgaag agaattgcca ccagatagaa acggacgtcg aacttcatgc gtgcatcgtc
     2281 gaatgcgtta aatccacatt catacgcgga gagcttttcc gtgtcgggat tggtcggcgc
     2341 aaaaagcgtt gtcaggacaa tgaagagcac gctcagcacc gccgcgatga cgagaaagat
     2401 aacgaccggc agatagctgg tgacgagcgc ttgcgcgtct tccatttaac cctcggattt
     2461 ggttgccgga cggcctattc cgctttgacg tgcgaggcaa tagaacgtcc ggaagcgtgc
     2521 gcgaatcttc gacagacgcg actttgtgac attaaatcta ggcgctgatc cgtttcctgc
     2581 agtgcgatgg aacgtcgcaa ttctgccggc cggctttctg caactggcgc gagtgacggg
     2641 gctcgaaccc gcgacctccg gcgtgacagg ccggcactct aaccgactga gctacacccg
     2701 cgcacagacg cgcacgtgat ggcgtgttgc gtcgttgggc gctggataag gggttcggtc
     2761 atgagtgtca agcgccgcaa catcgcctaa tgcgcttacc tagcgtgctt ttttcgcgtc
     2821 gcctcggaaa ccgacgcggc gatgctggag gccctgaatc ttcttgcgga accagaccga
     2881 agcgattaaa c
//
LOCUS       DASUOM010000026         5741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1197124,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000026 DASUOM010000000
VERSION     DASUOM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197124"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFY27_00545"
     CDS             <1..195
                     /locus_tag="VFY27_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764028.1"
                     /translation="EQRWRRSLKIFHRMSMHEPRFSLSRRHPAQLMPNKALQTDKGKV
                     SRRLHPQEARPLAFAAELDC"
     gene            271..639
                     /locus_tag="VFY27_00550"
     CDS             271..639
                     /locus_tag="VFY27_00550"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX5764029.1"
                     /translation="MDALIAAALAKPSERPKLLQALMSGHLFIIASWKDPKSRSITIQ
                     DFYRGEQSFLPVFSDEAHFKAEIAGSGFEDKGVSIDASLFASILKGGELLILNPGSIS
                     PIEISASELKKLVDPARLPK"
     gene            736..1143
                     /locus_tag="VFY27_00555"
     CDS             736..1143
                     /locus_tag="VFY27_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX5764030.1"
                     /translation="MYFDVYVNGKRIGSFGHADVENLSVSVSGAKDGHYLFAGVVCRE
                     DGKQFHYSWPQFELSASDEVRIVPSDGSALSEPERKYEMGRTERKAWEKNVCEFCQRN
                     ETQVERLIAGDRNRPGICSECVELCNAILKNHA"
     gene            1687..2220
                     /locus_tag="VFY27_00560"
     CDS             1687..2220
                     /locus_tag="VFY27_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764031.1"
                     /translation="MRIASSLIAIVLSLNASVVFAAERSPYSGQQSRQIKALSEATVQ
                     GLLKGQGLGYAKAAELNGYPGPRHVLELAAELSLTEQQVRETESLFQRMETEAQKWGK
                     ELVESEMHLDEIFARRLVNEESLDRALKRVTENSARVRAAHLSAHVAQAAVLSREQID
                     LYVKLRGYEGMQHGGEG"
     gene            2462..3262
                     /locus_tag="VFY27_00565"
     CDS             2462..3262
                     /locus_tag="VFY27_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5764032.1"
                     /translation="MRFDKARLSTGLRIHYAESGDARGTPVVFVHGWPDSWFSFSRVL
                     PLLPATLRLIAFDQRGFGESDHPDSGYTIPGFAADLVAFLDALDIERAVLVGHSYGTF
                     VARRAAIAMPERVVALALIGTGASTEIAGADDLQAALRELPDPIPEPFAREFQAGTVY
                     RPVPAEFFDRIIEESLKIPPHLWPVMIESLLAYDDTRELGSIRAPTQLLWGDHDALFS
                     RAQQDAFLAALPAARLKVYEETGHCPNWERPEWVADDIADFVAGAARG"
     gene            3307..3771
                     /locus_tag="VFY27_00570"
     CDS             3307..3771
                     /locus_tag="VFY27_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5764033.1"
                     /translation="MNSEFVALRGECRCGKVRFEITAPPIMTMACHCTGCQRMSASAF
                     SLSAAIPSDAFSITQGEPVIGGLRGALHHFFCDYCMSWLFTRVDGFESFVNIRVTMLD
                     DPSPFPPYIETYTSEKLPWANTPAVHSFSHFPPLDDYDGLVRQYASHVRAQT"
     gene            3818..4324
                     /locus_tag="VFY27_00575"
     CDS             3818..4324
                     /locus_tag="VFY27_00575"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5764034.1"
                     /translation="MPARKTDKSSGPAGRKPARRTAAPGKEARPLPESMVAGKASSAK
                     AAAGDKPVFAYIASLPEPQRSIAEGVDALAARTLPGLQRSVKWGMSYYGVGDGWCFCC
                     GGFADHVKLMFINGTALKPVPPVTPVAMGKATRGMELKSKDDLDERQVAAWMKQVAAV
                     PGVGGKKR"
     gene            4335..4643
                     /locus_tag="VFY27_00580"
     CDS             4335..4643
                     /locus_tag="VFY27_00580"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX5764035.1"
                     /translation="MLELRPSCENCAKALPADSVEARICSYECTFCSTCVDQVLMNVC
                     PNCGGGFVPRPVRPSRNWKDGNCLSNDPASEVVRHRPVDPEAHLAFAAAIRDIPPERR
                     "
     gene            complement(4672..5541)
                     /locus_tag="VFY27_00585"
     CDS             complement(4672..5541)
                     /locus_tag="VFY27_00585"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5764036.1"
                     /translation="MTDRWQAAARPGFITRLFDYSAEVTTPDRKSLDAAARLMPPNAR
                     VYVAALPNDAPDRQLAVCREVRDLGLIPVPHIVARNIPDRAALDLLLGRLAREAGVDR
                     ALILGGDRPVPAGDYDNSLQLIESGLLEKHGIGRIAIAGYPEGHPGIPDSALDAARKA
                     KLLAARAAGLEVIFITQLCFEAAPIVAWLRRLRAEGVTERVRIGVAGPASHATLLRFA
                     AICGVGASLRALKERPGRAKQLLAGETPERLILDLEAAATADPSLDIWGVHFFTFASL
                     ARTIEWVEQFRAG"
BASE COUNT         1092 a         1702 c         1812 g         1135 t
ORIGIN      
        1 gagcaacgtt ggagacgatc gttgaagatt ttccacagaa tgagcatgca cgaacctcgc
       61 ttcagtttat ccaggcggca tccggcgcag ttgatgccta acaaggcgct gcagacggac
      121 aaaggcaagg tgtcacgccg cttgcatccg caagaggcgc gcccgctcgc ctttgccgct
      181 gagcttgatt gttagggttc acttgaaacg atcgtggtta tgtctaccgt tgctcgcatt
      241 tttccacacg cgggtgctat cgaggggaac atggatgccc taattgccgc tgcgcttgcg
      301 aagccgtccg agcgcccgaa actactccaa gcgcttatga gcggtcatct cttcatcatc
      361 gcgtcttgga aagacccaaa gtcgcgttca attaccatcc aggactttta ccgcggcgag
      421 caatcatttc tgccggtgtt ttcggacgaa gcgcacttca aagcagagat tgccggttct
      481 gggtttgagg ataagggcgt gtccatcgac gcgagcctat tcgcgtctat tttgaagggg
      541 ggcgaattac taattctcaa tcctgggagc atttcaccga tagagattag cgccagcgag
      601 ttgaagaaac ttgttgatcc cgctcgactg ccaaagtgag ccctaacaaa tcgttggggc
      661 ggacgcgtga gagataaagt gccacgctca aacggcggcg tgcgcgccgc tcatctcaac
      721 cgttaggcgt cattcgtgta cttcgacgtt tacgtcaacg gcaagcgcat tgggtcattt
      781 ggacatgccg atgtcgaaaa tctctcggtg tccgtatccg gcgccaagga tggacactat
      841 ctgtttgccg gcgtcgtgtg tcgagaggat ggcaaacaat ttcattattc gtggccccag
      901 tttgaactgt cggcgagtga tgaggttcgg attgttccgt ccgacggaag tgccttgtca
      961 gaacctgaac gaaaatatga aatgggtcga acagagcgga aggcatggga gaagaacgtg
     1021 tgcgagttct gccaacgcaa cgaaacacaa gtggaaaggt tgatcgcggg agaccgaaac
     1081 cgacccggaa tctgctcaga gtgcgtggag ctttgcaatg cgattctcaa aaatcacgcc
     1141 taacaatgcg ctgcagctga cgtgtcaacc cgtcattccc cttgcaaaga tgataagcaa
     1201 ggggagcgcc ggcttgccac ggagctgagc ttagtcgtta ggcggcgggg aggcgtcatc
     1261 gatggatctg gtctcgtctg taaggcgtcg tgcgttggat actaacactc ggactgatca
     1321 gaccgatgtc tagccgaagg aggccaccat tttccacagt gatcgcggcg tcgagtacct
     1381 cgccggaagc taccgggagg tgcttacggc agcagctgga gcagagcgtg aaccggccgc
     1441 gcagaatgac tgacaacgct cacatggagt cctggaacaa gtcgatgaag tcggacatgt
     1501 accaccgtcg ccgcttttct gatgaccgat cactccgtgc ggccatcgga cggtacgtgg
     1561 acttttacaa ccatcagcgg ctacattctg ctctgggata ccggccgccg gtggagttcg
     1621 agcgtcaatg ttgctaacta atgggtgtcc actttttgcc ttcactttga gcctacggag
     1681 tttctgatgc gcatagccag ttctctcatt gcaatcgttc tttcgttgaa cgcaagcgtt
     1741 gtgttcgccg cagagcgctc tccttactcc gggcagcaga gccgacagat caaggcgctt
     1801 tccgaagcca cggtccaggg tctattgaag gggcagggtc tgggatacgc gaaagcagca
     1861 gagctgaacg ggtatcctgg tccaaggcac gtgcttgaac tggccgccga gctttcactg
     1921 acggaacaac aagtcaggga gactgaatcc cttttccaga ggatggaaac tgaggctcag
     1981 aaatggggca aggagctagt agagtcggag atgcacctgg atgagatatt tgcgaggcgc
     2041 ttggtaaacg aggaatctct cgatagggcc ttgaagaggg tcacggagaa ctcagcacgt
     2101 gttcgtgctg cgcacttaag cgcccacgtg gcgcaagcgg cggttctcag ccgcgagcaa
     2161 attgacctct acgtcaaact tcgtggctac gaaggtatgc aacacggcgg cgaaggctaa
     2221 ccatgcgttc aagcggaccg cggagagagg cattcatgtt tcctgatcgc attggaggaa
     2281 ttgcgcgctg atgatttacc gattcctccg gcgtccagtc gtgtccacta cgtcgaagtt
     2341 gccgcctgac acaaggcgct gcagacggac aaagacaagc tgtcatgcct cttgcattcg
     2401 aaaaatgcca cgccagcttg cctttgccgc tgcggttggt tgttagactg aggcactagc
     2461 aatgcggttc gacaaagctc gactcagcac gggtcttcgc atccactacg ccgagtctgg
     2521 cgacgcgcgc ggcacacccg ttgtgttcgt gcacggctgg ccggattcgt ggttctcgtt
     2581 ctcgcgcgtg ctgccgctac tcccggcgac gctgcgcctc attgcattcg atcagcgcgg
     2641 gttcggcgag tcggatcatc cggactcagg ctatacgatt cccggtttcg ccgcagacct
     2701 cgtggcgttt ctcgacgcgc tcgacatcga acgcgccgtt ctcgtcggtc attcgtacgg
     2761 caccttcgtc gcgcgccgcg ccgcgattgc gatgccggag cgcgtcgtgg ctctcgcgct
     2821 gatcggcacg ggggcttcga cggagatcgc cggtgccgac gatcttcaag ccgcgctgcg
     2881 cgagctgccc gatcccatcc cagagccgtt tgcgcgggag ttccaggcgg gtacggtgta
     2941 tcgcccggtg cccgccgagt tcttcgaccg catcatagag gagagcttga agatcccgcc
     3001 gcacctctgg cccgtaatga tcgagagcct gctggcttac gacgatacgc gcgagctcgg
     3061 cagtattcgc gctccgacgc agttgttgtg gggcgatcac gacgcgctgt tttcgcgcgc
     3121 ccagcaagac gccttcctcg cagccctgcc tgcggcgcgg ctcaaggtct acgaggagac
     3181 aggtcactgc ccgaactggg agcgtcccga atgggtcgcc gacgatatcg cagacttcgt
     3241 cgcgggcgcg gctcgcggct agccgctgcg ggaatgcggt cgccgaacgg cagacgggag
     3301 cagatagtga acagtgagtt cgtggcgttg cgaggggagt gtcgatgcgg aaaggtccgc
     3361 ttcgagatca ctgcgccgcc gattatgacg atggcctgcc attgcacggg gtgccagcgt
     3421 atgagtgcca gcgcattctc cctgagcgcg gcgattccaa gcgatgcctt ctcgattacg
     3481 caaggggagc ccgttattgg tgggttgcgc ggagcgcttc accacttctt ttgcgactac
     3541 tgcatgagtt ggctctttac gcgagttgat gggttcgaat cgttcgtcaa tattcgagtg
     3601 acgatgctcg atgatccgag cccgtttcct ccgtacatcg aaacatatac aagtgaaaag
     3661 ttgccttggg caaatacgcc ggctgttcat agtttttcgc actttccgcc gctggacgac
     3721 tatgacggcc tggtcaggca gtacgcctcc catgtcaggg cacaaactta agctgcgtcc
     3781 gtgttgccta tacactcccg aggtacagga gaccaccatg cccgcccgaa agacagacaa
     3841 gagttccggt cccgccggcc ggaagccggc caggcgcacg gcggcaccag gcaaagaggc
     3901 caggccgctg ccggaatcga tggtagccgg gaaggcaagc tcggcgaagg ctgcggccgg
     3961 cgacaagccg gtcttcgcct acatcgccag tttgccggag ccacagcgga gcatcgcgga
     4021 aggtgttgat gctcttgcgg ccaggacgct ccccggcctg cagcgctccg tgaaatgggg
     4081 catgtcctac tatggcgtcg gcgacggctg gtgcttctgt tgcggcggat tcgccgacca
     4141 cgtcaagctc atgttcataa acggcacggc gctgaagccc gttcccccgg tgacgccggt
     4201 cgcgatgggt aaagcgacgc ggggcatgga gctcaaatca aaggacgacc tcgacgagcg
     4261 ccaggtcgcg gcatggatga agcaggtcgc ggccgtgccc ggtgtcggcg ggaagaagcg
     4321 gtaggcgtcg ccccatgctg gaactccgtc ccagctgcga gaactgcgcg aaggcgcttc
     4381 cggcggattc tgtcgaggcg cggatatgca gctacgagtg cactttttgt tcgacctgcg
     4441 tcgaccaggt gctcatgaac gtctgtccga actgcggagg cgggtttgtg ccgcgcccgg
     4501 ttcgcccgtc tcgcaactgg aaggacggta actgcctgtc aaacgatccc gcgagcgaag
     4561 tggttcgcca caggcccgtc gaccccgagg cgcatctcgc gttcgcagcg gcgattcgag
     4621 acatcccgcc ggagcggcgt tagcctgatg ccgattcgcc gggacgtcct gtcacccggc
     4681 ccggaactgc tccacccact cgatggtcct ggcaagcgac gcgaacgtga aaaaatgcac
     4741 accccagatg tcgagcgacg ggtctgccgt cgccgcggct tcgaggtcca ggatgaggcg
     4801 ctcgggtgtc tcgcccgcga gcaactgttt cgcgcgcccg gggcgttcct tcagcgcgcg
     4861 cagcgaagcg ccgacgccgc agatcgccgc gaagcgcagg agtgtcgcgt ggctcgccgg
     4921 gccggccacg ccgatgcgca cgcgttcggt gaccccttcg gcgcgcagcc ggcgcagcca
     4981 ggcaacgatg ggcgccgctt cgaagcacag ttgcgtgatg aaaattactt cgaggccggc
     5041 agcgcgggcg gccaggagct tggcttttcg cgccgcgtcg agcgcgctgt ccgggattcc
     5101 cgggtggcct tcgggatagc ccgcgatggc gatccgcccg atcccgtgtt tctccagtaa
     5161 tcccgactcg atcaattgca ggctgttgtc gtaatcgccg gccgggactg ggcggtcgcc
     5221 gccgagtatg agcgcccggt cgacgccggc ttcgcgggcc agccggccga ggagcaggtc
     5281 gagcgcagcc cggtccggga tgttgcgcgc cacgatgtgc ggcacgggga tgagccctaa
     5341 atcccgcacc tcgcggcaga ccgcaagctg ccggtccggc gcgtcgttcg gcagcgcagc
     5401 gacatagacg cgcgcgttcg gcggcatgag ccgagccgcc gcatccagcg atttccggtc
     5461 gggcgtcgtg acctcggccg agtagtcgaa gaggcgggtg atgaaaccgg ggcgtgccgc
     5521 ggcctgccac cgatcggtca ctacctggcg ctcttacgct ggaggcccat gcctggcgct
     5581 ctcctcaggc cgcgccggta cgccagccgg ttgcgtaggc ctcgcgggcc actttggagt
     5641 acggcaccgg gctcaccacg gcccgcacct ccgtctgctc gtgccgctcc actgtcagtt
     5701 tgtccgtgcc gccgccgggc tcgccccaga cgatcgttac t
//
LOCUS       DASUOM010000027         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_954668,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000027 DASUOM010000000
VERSION     DASUOM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954668"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..792
                     /locus_tag="VFY27_00590"
     CDS             520..792
                     /locus_tag="VFY27_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764037.1"
                     /translation="MDEDLEQMTRDDLLAEARKLRQGIREHRDSSRHALCWHHPALWG
                     LLPEQTDPVPVVPEWPVFLEGCIRYRQSLDAQAPRAPRSNRPYEHG"
     gene            824..1378
                     /locus_tag="VFY27_00595"
     CDS             824..1378
                     /locus_tag="VFY27_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5764038.1"
                     /translation="MPTLHTPRLLLREFALSDAADVQRLASDYAVADTTQNIPHPYPD
                     RAAAEWISTHGLAFEAGDAATFAIVLRQEDALAGTAGIRFDLRSDSAELGYWIGRPYW
                     GRGYCTEAALAVLEYAFGDLNLNRVHASHLLRNPASGRVMQKLGMTREGLRREHAKKW
                     NKYEDLVEYGILRREWRDQALKPG"
     gene            complement(1449..2018)
                     /locus_tag="VFY27_00600"
     CDS             complement(1449..2018)
                     /locus_tag="VFY27_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5764039.1"
                     /translation="MRSALCLCAFVMSFAGVAQAADNGIYLGGGVTRTSIDTGNDFIE
                     SAPDFSLDDDDNGFKVIAGMRPFDWLAFEANYVDFGNIEVSSTGASVPGGSFELTGID
                     AFAVGFLPVTFVDIFGKVGAIRWDSDSAANVGGIDFEDSDSGTDLAYGAGVQARLGSL
                     AARLEYERFEVDNTDEVAMITLGLTYTFL"
     gene            complement(2343..3200)
                     /gene="bla"
                     /locus_tag="VFY27_00605"
     CDS             complement(2343..3200)
                     /gene="bla"
                     /locus_tag="VFY27_00605"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX5764040.1"
                     /translation="MDRRRFLAATSLYLAGSMFARASTESPFADIEAVLGGRVGVMAV
                     DTGNGSVMSHRADERFAMCSTFKWVLAAFVLSQVDQDEMSLDERLSYGPDDLLEYAPV
                     ARKYVDRGWLTVNALCGATVTVSDNTAANLLLHRLGGPPELTAFLRHCGDNATRLDRN
                     EPDLNTNLPGDERDTTTPRAMTGTMNSILLGDEILSAASRARLIGWLKQTSTGLERLR
                     AGLPGDWIAGDKTGTGVNGAANDVAIAWPPDRNPILIAAYLSESAASPDALNTAQADI
                     ARAVAARLG"
     gene            complement(3200..>4279)
                     /locus_tag="VFY27_00610"
     CDS             complement(3200..>4279)
                     /locus_tag="VFY27_00610"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX5764041.1"
                     /translation="RIPDIDYFSTAPDDASNDDVALLHAQEYHTSYWGHMGLLGLGEH
                     YLLPDYSSYPGTAAASLHPDNVTAAGMARSQGAVVGYVHPFDAPAPDPETAERLTNSL
                     PVNAALGLLDYYEVVGFADHRTSAEVWYRLLNCGFRIAAAGGTDAMANYASLRGPVGM
                     NRTYVEMPHENLEPAARRDAWLSGLKNGQSLATNGPLLGLTVNGEGPGGDVALGAGDN
                     ELTYSGFLRSMVPVDHLELVYNGKVVKAFDLRGEQKSADLSGSLQVDESGWLLVRAWN
                     EGSHPSVFDLYPYATTTPVYVSIDGRAPRSAEDAGYFIAWIERIRESAAEHPGYNSDK
                     ERALVLAHLDRAQQVFENRRGDGVH"
BASE COUNT          784 a         1320 c         1423 g          752 t
ORIGIN      
        1 accagaagcc ggcctcgcgc atcgacacgc ggtgtggccc ctgcttgtgc atggtggtga
       61 aatcgacgaa gagtgccacc accacgacca gcacgaaaag cagccacatg aatggcgaac
      121 cgatggatga cattgggacg agcctcgagg gcggttggcg tgaaccgtgg gtagttgatc
      181 aatgcccatg ccggtcttgc cagccgcgac ctttgcgcgg actgcagtgc cgggtaaacc
      241 gatttggcgc cgaccgaatc gatcgtccgt gctgacgaca ctgcaggagc gcacgaatgc
      301 gccccggcta ctccccttga gaggcgcggt gattatacag cctgccggga aagtccatgg
      361 gttcaatttc cggggccggg cagcggtttc gggagctcct ggcgaacggg ccttccacgg
      421 gaatggctgc atgtggtctg tggggagcgt tcctcgcttg tcggccgcac tgccggaggt
      481 tactttcgac ctcttttccg cttgaaacgg agagatgcgg tggacgaaga cctcgaacag
      541 atgacgcgcg acgacctgct tgccgaggcc aggaagctcc ggcagggcat ccgcgagcat
      601 cgtgacagca gcaggcacgc cctctgctgg catcatccgg cattatgggg gctgctgccg
      661 gagcagaccg atcctgttcc cgtggtcccg gagtggccgg tattcctgga gggctgcatc
      721 cgctaccggc agtcgctcga tgcgcaggcg cccagggcgc cgcggagcaa ccggccgtac
      781 gagcacgggt aaagggtcga cctgggggca gtccgtgaga cgaatgccga ccttgcacac
      841 gcctcgattg ctccttcggg aattcgcgct gtccgatgcc gcggatgtgc agcgcctggc
      901 cagcgactac gcagtcgccg atacgacgca gaacattccc cacccctatc ccgatcgtgc
      961 cgccgcggaa tggatctcca cgcacggtct cgcgttcgaa gccggcgatg cggcgacctt
     1021 cgcgatagtg cttcgacaag aggatgcgct ggccggtacc gcaggtatca ggttcgatct
     1081 ccgctccgac agcgcggaac tcggctactg gatcggccgc ccgtactggg gacgcggcta
     1141 ctgcaccgag gcggcgcttg ccgtgctgga gtacgccttc ggcgacctca acctgaaccg
     1201 tgtgcatgcg tcgcacctcc tgcgaaaccc ggcgtcgggg cgggtcatgc agaagctcgg
     1261 catgacccgg gaaggcctgc gccgggagca tgcgaagaaa tggaacaagt acgaggacct
     1321 cgtcgagtac ggcatcctgc ggcgggaatg gcgggatcag gccctgaaac ccggctgacg
     1381 caggtcgtgg cggggtcacg atggagacag tgatggcggg ccccgggtga gcccgccacc
     1441 gcctgcactt acaggaacgt gtacgtcagg ccaagcgtga tcatcgcgac ttcgtcggtg
     1501 ttgtcgacct cgaagcgctc gtattcgagc cgggcggcaa ggctgcccag tcgcgcctgg
     1561 acgccggcgc cgtaagctaa gtcggtgccg gaatcgctgt cttcgaagtc gatgccgccg
     1621 acgttggcgg cggaatcgct gtcccagcga atggcgccca ccttgccgaa gatgtcgacg
     1681 aaggttacgg gcaggaagcc gaccgcaaat gcgtcgatac cggtcagctc gaaagacccg
     1741 cccggaacgg atgcgccggt actgctcacc tcgatattcc cgaaatcaac gtaattcgcc
     1801 tcgaaggcca gccagtcgaa ggggcgcatg ccggcgatga ccttgaagcc gttgtcgtcg
     1861 tcgtcgaggc tgaagtccgg agccgactcg atgaagtcgt tcccggtgtc gatgctggtg
     1921 cgggtcacgc caccgccaag gtaaatgccg ttatcggctg cttgcgctac gccagcgaaa
     1981 gacataacga atgcacacaa acataacgct gatctcatgg atataactcc tttacggctg
     2041 ggggatctga caaaaaaagg ggacttcgca ggaagacaat ctgtgccgga cagacgtggc
     2101 tacaagatcg acgatgcgcg cgccggcgcg gggactaaag cgccaggcct tcctgaaatt
     2161 caacagtaga tgtgcgtgcg cccggggacc cggacggcag ctgcactgta gctgcaatct
     2221 gggacaccca tcaagtcgga aggttcgcta ggatgcagat cccataccaa tcaggggttt
     2281 atggtcacag cggaattcgt gggtgtcccc gttccggggc tgtgggtgtc cctgttccgg
     2341 ggctagccca ggcgagcggc gacggcgcgg gcgatgtcgg cctgggcggt gttgagggcg
     2401 tcggggctgg cggccgattc ggagaggtag gcagcgatga ggatggggtt ccggtcgggc
     2461 ggccaggcta tggcgacgtc gttggcggcg ccgttgacgc cggtgccggt cttgtcgccg
     2521 gcgatccagt cgccggggag accggcccgc aggcgttcga ggccggtcga ggtctgtttt
     2581 agccagccga tgagcctggc gcggctggcc gcggacaata tctcgtcgcc caggaggata
     2641 ctgttcatgg ttccggtcat ggcacgcggc gtcgtcgtgt cgcgctcgtc gccgggcagg
     2701 ttcgtgttca ggtccggctc gttgcggtcg aggcgcgtgg cgttgtcgcc gcagtggcgc
     2761 aggaaagccg tgagttccgg cggacccccc aggcgatgca gcagcaggtt cgcggcggtg
     2821 ttgtcgctga ccgtgacggt cgcgccgcac aacgcgttga cggtaagcca cccacggtcg
     2881 acgtacttcc tggcgacagg cgcgtattcg aggagatcat cgggaccgta gctcaggcgc
     2941 tcatccagcg acatctcgtc ctggtctacc tgcgacagta cgaatgcggc tagcacccat
     3001 ttaaacgtgg agcacatcgc gaagcgctcg tccgcgcgat gcgacattac ggacccgttg
     3061 ccggtatcca cggccatcac gccgaccctc ccgccgagga cggcttcgat gtctgcgaaa
     3121 ggcgattccg tggaggcgcg ggcgaacatg ctgccagcaa ggtaaagact ggttgctgcc
     3181 aggaagcgac gtcggtccat cagtgcaccc cgtctccccg ccggttttcg aatacttgct
     3241 gcgcccgatc aaggtgagcg agcaccaagg cgcgctcctt gtcgctgttg tagccgggat
     3301 gctccgcggc cgattcgcga atgcgctcta tccacgcgat gaaatacccg gcgtcttcgg
     3361 ccgatcgcgg cgccctgccg tcgatgctca cgtaaaccgg cgtggtcgtt gcgtagggat
     3421 aaagatcgaa cacggatgga tgcgaaccct cgttccacgc acgcaccagc agccatccgc
     3481 tttcgtccac ttgcaggctg ccgctcaggt ccgcgctttt ttgttctccg cgaaggtcga
     3541 aggccttgac gaccttcccg ttgtacacca gttccaggtg atcgaccggc accatggagc
     3601 ggagaaagcc ggagtaggta agctcgttgt cgccggcgcc aagcgcgacg tcgccgccgg
     3661 gtccttcgcc gttgaccgta aggccaagga gaggaccgtt ggtggcgagc gactgtccat
     3721 ttttcaatcc ggaaagccag gcatcccgcc gcgccgccgg ttccaggttt tcgtggggca
     3781 tctcgacgta agtgcggttc atgccgaccg ggccgcgcag cgaggcatag ttcgccatcg
     3841 cgtcggtgcc gcccgctgcc gctatgcgaa agccgcagtt cagcaagcga taccacacct
     3901 ccgccgaggt gcgatgatcg gcgaagccga ccacctcgta gtaatccagc aagccgagcg
     3961 ccgcattgac cggcagcgaa ttcgtcaacc gctcggccgt ctccggatcc ggcgccggcg
     4021 catcgaaagg atgcacgtag cccacgacgg caccctgcga gcgcgccatg ccggcagcag
     4081 tcacattgtc gggatgcaga ctcgccgcgg ccgtgccggg ataagaggaa tagtccggca
     4141 acaggtagtg ctccccgagg ccgagcagtc ccatgtgacc ccagtaactg gtgtggtact
     4201 cctgcgcgtg caacaaggcc acgtcgtcgt tggacgcatc atccggcgcc gtcgagaagt
     4261 agtcgatatc gggaattcg
//
LOCUS       DASUOM010000028         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_831974,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000028 DASUOM010000000
VERSION     DASUOM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831974"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFY27_00615"
     CDS             complement(<1..209)
                     /locus_tag="VFY27_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764042.1"
                     /translation="MTSEELPTTRPGDSRQLTAGEVELAHSVFGDAIDYARVRIFRRK
                     YFPLQPRNRTMAPNGNIYFHPRSLA"
     gene            complement(231..1355)
                     /locus_tag="VFY27_00620"
     CDS             complement(231..1355)
                     /locus_tag="VFY27_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5764043.1"
                     /translation="MRKLLRKGSPEKAEIDAFIAREDFPLVDGNEVTFVYRGAADAVY
                     LRCWISGFDTAQPFQSLNDTDLWVSTIDLPPGSRIEYKLEVEAGGKRELILDPLNDVR
                     AHDPFGANSVCQGAGYERPAWTFPDPEARSGSIDMFDVESSAFRESRGVSAYLPARFR
                     RNRRYPLLVVHDGHDYVRYAELKVVLDNLIHYLEIPQMIVALTQSPDRLKEYGGHDAH
                     ADFIATDLLPQLADRYPLVDSVTARGMMGASFGGVAALHAAWRYPDLFRRLLLQSGSF
                     AFSDLGGHRRSPVFDPVAKFMNAFRENPGDISGKVYMSCGMYESLIYENRSLLPRLQA
                     QGMDVRFEEVRDAHNWENWRDRLRNGLSWLFPGPLWMVYE"
     gene            complement(1364..2296)
                     /locus_tag="VFY27_00625"
     CDS             complement(1364..2296)
                     /locus_tag="VFY27_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX5764044.1"
                     /translation="MPQIILLGPQRPNTNLGAAIVEAGLPDGSIAVISAGWQEAEGDI
                     EDIHELVRRPLHDLRLYQRAEEVFAAAPELLLAYRARQDRLQVLQRLYRLRLRRLLNA
                     ARQMLRAKVELSLVEAEQRHAIAQLRALDRHHMRRVSVVNEEFDAEFDVGKHELLARH
                     VAEISGILDNVETVVITGGNVAILINRLRLFGLGDMLKAKNVVAWSAGAMALCDRIVL
                     FHDNPPQGRRNPEVLGPGLGLVRRQVLFPNARTRLRTSDRVRMQLLARRFAPAACVTL
                     DSGAILRIADGRFVSVERARRIMESGRLRKVKTP"
     gene            complement(2301..3341)
                     /locus_tag="VFY27_00630"
     CDS             complement(2301..3341)
                     /locus_tag="VFY27_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5764045.1"
                     /translation="MQDVTAESDRKVALWLLTLCALVYGMILLGGVTRLSGSGLSIVD
                     WRPIMGVLPPLSEQDWQQTFDMYKESPEFRKVNAHMTVQDFKGIFWLEYLHRLLGRLI
                     GIAFLLPFLYFVWRGYIRKHEWPRYGLMFVLGGLQGVLGWYMVKSGLIDNPRVSQYRL
                     TAHLLAAIAIYAYMFWIALSLLSPYKVHATHPWFSRTLLLAVLVLTTIISGGFVAGLD
                     AGLFYNTFPKMGNYWIPPDVFHLSPWWRNLFENMAAVQFNHRVLALTTFALIIVYWLY
                     ARRADLPTRLGRGVNALLHTAVLQVILGISTLLLVVPLVLAAAHQAVAVLLFTVMLYL
                     CHEMRRAAAAPS"
     gene            complement(3534..3884)
                     /locus_tag="VFY27_00635"
     CDS             complement(3534..3884)
                     /locus_tag="VFY27_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764046.1"
                     /translation="MKAINLAEKLATFTEHWQPRTIGQFNGHDLLVVKAQGEFNWHKH
                     DDTDDLFLVLRGRITIQMRDGDVTVGPGELFVVPKGVEHCPFAEDEAHILLIEPTGTP
                     NTGNAETAAPRREI"
     gene            3912..>4702
                     /locus_tag="VFY27_00640"
     CDS             3912..>4702
                     /locus_tag="VFY27_00640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5764047.1"
                     /translation="MSPAAYIEPADSCDSLAAHRTGLGQRNAVRLKNCFNTADFRTLA
                     KARLPSPIFHYIDGGSDDETTLRRNTEAFNSCDLVPSVLAGVESADLRTHVLGQELSM
                     PLFFSPTAMQRLFHHDGERAIANVAKKFGTMFGVSTIGTCSIEELGSFEDVPKLFQIY
                     VHNDPGLTTDLITRCKNSRFSALALTVDTIVAGNRERDLVTGMTTPPKLTVRSLMSFL
                     WHAEWTLNYLTRERFDLPSVSRYVEQGSSVSSSVAAYIDSQMKRS"
BASE COUNT          939 a         1487 c         1399 g          877 t
ORIGIN      
        1 taggcaagcg agcgaggatg aaagtagata ttgccattcg gcgccatcgt tctgttgcgc
       61 ggctgtagcg gaaagtactt tctgcggaaa attcgtaccc gcgcgtaatc gatggcatcg
      121 ccgaagacgg agtgcgcaag ctcgacttcg ccagcggtca gctggcgaga gtcgcctggc
      181 cgcgtcgtcg gaagttcttc gctcgtcatt tttgctaccg ttcccggaag ctattcatac
      241 accatccaca gcggtccggg aaacagccac gaaagaccat tccgcagccg gtcacgccag
      301 ttttcccagt tgtgagcgtc acgcacctct tcgaaacgca cgtccatccc ctgtgcctgc
      361 aggcgcggca gcaacgagcg gttctcatag atgagcgact cgtacatgcc gcagctcatg
      421 tagaccttgc cggaaatatc gcccgggttt tcgcggaacg cgttcatgaa tttcgccacg
      481 ggatcgaaaa cgggcgaacg gcgatggccg ccgagatcgc tgaaggcgaa cgaccccgat
      541 tgcaggagca gccgccggaa aaggtccggg tagcgccacg ccgcatgcag tgccgccacc
      601 ccgccaaagc tcgcgcccat cattccgcgt gccgtgacgg agtcgaccag cggataacgg
      661 tcagcgagct gcggaagcag gtctgtcgcg atgaaatcgg catgcgcatc gtgcccgcca
      721 tactccttca ggcgatccgg cgactgcgtc agcgcgacga tcatctgcgg aatctcgagg
      781 tagtggatca ggttgtccag aactacctta agctccgcat agcgtacgta gtcatgtccg
      841 tcgtgcacga ccagcagggg atatcggcgg ttgcggcgaa aacgcgccgg gagataggca
      901 cttaccccgc gggattcccg aaacgcgctg ctctccacat cgaacatatc gatagaaccg
      961 ctgcgcgctt cgggatcggg aaaggtccac gcgggtcgct cgtaacctgc cccctggcaa
     1021 acggaattcg cgccgaacgg gtcgtgcgcc cgtacgtcgt tcagcgggtc gaggatcagc
     1081 tcgcgtttgc cgccggcttc cacctcgagc ttgtactcga tccttgaccc gggcggcagg
     1141 tcgatagtgg agacccagag gtcggtgtca ttgagcgact ggaaaggttg cgccgtgtcg
     1201 aatcccgaaa tccagcagcg caggtaaacc gcatccgcgg cgccgcggta gacgaacgtc
     1261 acctcgttcc cgtcgaccag cgggaagtcc tctcttgcga tgaaggcatc tatttccgcc
     1321 ttttccggag agcctttccg caacaatttg cgcaatgagg gattcacggc gtctttacct
     1381 tgcgcagcct gccgctttcc atgatgcgcc gggcgcgctc gacggacacg aatcgaccat
     1441 cggcgatgcg cagaattgcg ccgctgtcca gcgtaacgca cgctgccggc gcgaaacggc
     1501 gggcgagaag ctgcatcctc acgcgatcgg aggtcctgag ccgggtacgg gcattgggaa
     1561 agaggacctg gcgccgcacg agtccgaggc ccggaccgag gacttcgggg ttgcgccgtc
     1621 cctgcggtgg attatcgtga aaaagaacga ttcgatcgca cagcgccatg gcgccggcgg
     1681 accaggcgac cacattcttt gccttcagca tgtctcccag tccgaacagt cgcaggcggt
     1741 tgatgagtat cgcgacgtta ccgccggtta ttacgacggt ttcgacgttg tccagtattc
     1801 cggatatctc agcaacgtgg cgcgccagca gttcatgctt ccctacgtcg aactccgcat
     1861 cgaattcctc gttgaccacc gaaacccgcc gcatgtgatg ccggtcgaga gcgcgaagct
     1921 gtgcaatagc gtgccgctgc tccgcctcga ccagcgaaag ctccaccttt gcgcgcagca
     1981 tctgccgtgc tgcgttcagc aggcggcgca ggcgcagcct gtacagcctt tgcaggacct
     2041 gtagcctgtc ctgccgtgcc cggtaagcga gcagcaactc cggcgccgcc gcgaacactt
     2101 cctcagcgcg ctggtaaagc cgcaagtcgt ggagcggacg ccggacgagt tcgtggatgt
     2161 cctcgatgtc gccttccgcc tcctgccagc ccgcggagat gacggcaatc gaaccgtccg
     2221 gcaagcctgc ttcaacgatg gcggcgccca ggtttgtgtt cgggcgctgc gggccgagga
     2281 ggatgatctg cggcatttgc tcaggacggg gcggcggcgg ctcgccgcat ctcgtggcac
     2341 aggtacagca tcacggtgaa cagcagcacc gccactgcct ggtgagccgc cgccagcacc
     2401 agagggacca ccagcagcag cgtcgagata ccaaggatga cctgcagcac cgcagtgtgt
     2461 agcagggcgt ttacgcccct tcccagccgc gtgggcaggt cagccctgcg cgcatataac
     2521 cagtacacga tgatcagcgc aaacgtcgtc aacgccagta cccggtgatt gaactgcact
     2581 gccgccatgt tctcgaacag gttccgccac cacggtgaca ggtggaatac gtccggcggt
     2641 atccagtaat tccccatttt cgggaacgtg ttgtagaaca ggccggcatc gagaccggcg
     2701 acgaaaccgc cggagatgat cgtcgtcagt accagcaccg cgagcagcag tgtccgtgaa
     2761 aaccacggat gggttgcatg aaccttgtac ggagacagga gcgacagggc aatccagaac
     2821 atgtacgcgt agatcgcgat cgccgccagc agatgcgccg tcaggcggta ctggctgacg
     2881 cgcgggttgt cgatgagccc gcttttgacc atgtaccagc ccaacactcc ctgcagcccg
     2941 ccgagcacga acatcaatcc atatctcggc cattcgtgtt tccggatata cccgcgccac
     3001 acgaagtaca ggaagggcag gagaaaggcg atgccgatca gccggcccag caaacggtgc
     3061 aggtattcca gccagaagat gcccttgaaa tcctgcacgg tcatgtgcgc attgaccttc
     3121 cggaactccg gcgactcctt gtacatgtcg aacgtctgct gccagtcctg ctccgaaagc
     3181 ggcggcagga ctcccatgat gggacgccag tcgacaatcg agagcccgga accgctcagg
     3241 cgagttaccc cgccgagcag gatcatgccg tacaccagcg cacacagggt cagcagccag
     3301 agtgctacct tgcggtcgct ttccgcggtt acatcctgca ttgcctggtt acctggaata
     3361 agacttgaga cggcgcgcaa tagtagcagt cagcggctta cggcgccgca gcacaagacg
     3421 agtgcaggag cccgccacgc gggcgagttc tcgaatgccc atcgaactcg ccagcatcgc
     3481 gggctccctc ctgcgccttc ggttgtgcaa gtgttgcccg ttcccggact ttgctagatc
     3541 tctcgcctgg gagcagcggt ttcggcattg ccggtattgg gcgtgccggt cggctcgatg
     3601 agcaggatgt gcgcttcgtc ctcggcgaaa gggcagtgct cgacgccttt gggcactacg
     3661 aacagttcgc cggggcctac agtcacgtca ccgtcccgca tctggatcgt gatccggcct
     3721 ctcagcacca ggaacaagtc gtccgtgtcg tcatgcttgt gccagttgaa ctcaccttgc
     3781 gctttcacaa ccagcaaatc gtgtccgttg aactggccga tggtccttgg ctgccagtgt
     3841 tcggtaaacg tggcgagctt ttcagcgagg ttgattgctt tcacgaatga ggtctccgaa
     3901 ggagtcgaag ggtgtcaccg gcagcgtaca tcgagcctgc cgactcatgt gatagtcttg
     3961 ccgctcatcg aaccggtctc ggccagcgga atgccgtgcg tctgaagaat tgctttaata
     4021 ctgccgactt tcgcacgctc gcgaaagcac ggctgccatc tccgatcttc cattacatcg
     4081 acggcggctc cgacgacgag accacgctgc gccgcaacac ggaagcattc aactcctgcg
     4141 atctcgtgcc gagcgtgctg gcaggggtcg agtcggcgga cctgcgcacg catgtgctcg
     4201 gacaggagct cagcatgccc ctgttcttct ctcccacggc catgcagcga ctgttccatc
     4261 acgacggcga acgtgcgatc gccaacgtcg caaagaagtt cggcacgatg ttcggcgtgt
     4321 ccacgatcgg cacctgcagc atcgaagaac tgggtagttt cgaagatgtc ccgaagctct
     4381 tccagatcta cgtgcacaac gacccgggcc tcacgaccga cctcatcacc cgttgcaaga
     4441 actccaggtt cagcgcgctg gccctgacgg tcgatacaat cgtagcgggc aaccgggagc
     4501 gtgacctcgt taccggcatg accacgccac cgaagctcac cgtgcgcagc ctcatgagct
     4561 tcctgtggca cgcagagtgg accctgaact acctcacccg cgagcgcttc gatcttccaa
     4621 gtgtctcgcg ctacgtcgag cagggttcca gcgtctcgtc ttccgtcgcg gcctacatcg
     4681 acagccagat gaagcgatcc at
//
LOCUS       DASUOM010000029         7184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1436413,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000029 DASUOM010000000
VERSION     DASUOM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436413"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY27_00645"
     CDS             <1..345
                     /locus_tag="VFY27_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764048.1"
                     /translation="VVEVLLRKSDRLYLNDGMYGIFWELRFRGHDRYPVKAYRDGAEL
                     QGALRSYKLYGPTCDGSDMLPGQVDLPADTRPGDYLEFGRIGAYSLSGRTNFNGHYSD
                     TVVTIEAGTPPG"
     gene            complement(396..1304)
                     /locus_tag="VFY27_00650"
     CDS             complement(396..1304)
                     /locus_tag="VFY27_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5764049.1"
                     /translation="MDRFRAIETFARVVESGSFARAAERLDLSTSAVSRKVSDLEAHL
                     EARLLNRTTRRLSLTEAGRSFYERSVQLLSDLEEAELAMRAAAVTPKGTLKLTCGVSF
                     GERFIAPAIGEFAAQHQQVVFDLDLSDRPMDLVEEGFDMAIRIGSVSQQGLVSRRLGW
                     TQIVCCASPAYLAGRGTPQTPEELAEHDCLSYTLVPVPNEWRFEDSQGESYALSVSPR
                     HRANNGRMLAAMAAADLGVIIEPDFIVSPEVKSGELVRLLADYSLPRAPIAALYPSRR
                     QLSAKVRTFVDFLAARFSSEQAWHLD"
     gene            1412..2044
                     /gene="gstA"
                     /locus_tag="VFY27_00655"
     CDS             1412..2044
                     /gene="gstA"
                     /locus_tag="VFY27_00655"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX5764050.1"
                     /translation="MKLYYTPGACSQAPHIALHELGLPFEAVQVDLATHKLPDGSDYY
                     AVNPKGYVPFLVLEDGTDLSEAHVILQYVADREPGALAPEFGTIERWKLMEWLGFVAT
                     EVHKGFGPLWSPQTPPEVRERTVSALGNRFTVLAETLGRQPFLTGEKFTIADAYLFVV
                     LNWTKVHKVDLKPWPALSAFQARVAARPSVQATLEDEGLMARKRPARAAG"
     gene            2408..4330
                     /locus_tag="VFY27_00660"
     CDS             2408..4330
                     /locus_tag="VFY27_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX5764051.1"
                     /translation="MSIFANYQRRYEDTEQEEFSLQEYLEICKTDRTAYASAAERLLI
                     AIGEPQLVDTSKDPRLSRIFSNKVIKRYPVFDDFYGMEECIEQIVSFFKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKLLMEVVPIYSLKGSPVQESPLGLFKPAEDARILRED
                     YGIPERYVNTIMSPWAAKRLKEFNGDITKFRVVKRHPSILNQIAISKTEPGDENNQDI
                     SSLVGKVDIRKLEEFPQNDPDAYSFSGGLCRANQGILEFVEMFKAPIKVLHPLLTASQ
                     EANYNGTESVGSIPFDGIILAHSNEAEWQAFKNNRNNEAFIDRVYIVKVPYCLQVTEE
                     VKIYEKLLRNSSLSEAPCAPDTLRMLAQFSVLTRLVEPENSSIYSKMRVYDGENLKDI
                     DPKAKSIQEYRDTAGVDEGMEGLSTRFAFKILSKVFNFDSTEIAANPVHLLYVLEKQI
                     EHEQFPADVRDRYLNFIKEFLAPRYIAFIGKEIQTAYLESYSQYGQNIFDRYITYADL
                     WIQDQDYRNPDTGENLDHSALNDELEKIEKPAGISNPKDFRHEVVNFVLRARAANHGK
                     NPAWTSYEKLRLVIEKNMFSSTEDLLPVISFNPKASTEDKKKHKDFVSRMTEYGYTEK
                     QVRLLSEWYLRVRKSR"
     gene            4344..5591
                     /locus_tag="VFY27_00665"
     CDS             4344..5591
                     /locus_tag="VFY27_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEX5764052.1"
                     /translation="MIIDRREEGRNKSAVNRQRFLRRFRKHIKEAVADAVDKRSITDI
                     DRGDEISIPRKDVSEPVFRHGSGGRQRRILPGNREFIAGDRIKRPVGGQGGGGGQASN
                     TGEGLDDFAFELSRQEFLDFMFEDLALPNLVKKQLKDTETFRYQKGGYTNVGIPARLN
                     VVQSLRNAHARRIALTGATKRRMDALREELEGLQDRAGADQRMEEIRLELERLERRLN
                     QIPFLDDFDLKYNNLIQQPLPTSNAVMFCLMDVSGSMTQEVKDVAKRFFILLHLFLKR
                     AYKKIDVVFVRHHTVASEVDEDEFFHSRETGGTVVSSVLKLMLEIIRERYPSADWNIY
                     AAQASDGENWPDDSPIARDLLLNGILPLVQYYAYVEITKYDHQELWHAYEAVQEKKPD
                     CFAMKKVTDASDIFPVFHELFKK"
     gene            5597..7126
                     /locus_tag="VFY27_00670"
     CDS             5597..7126
                     /locus_tag="VFY27_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX5764053.1"
                     /translation="MVERRPIAETSEWTFENLADFERELARIAKQYRLDTYSNQIEII
                     TAEQMMDRYSSVGMPLGYHHWSLGKQFLNTEQKYRRGHMGLAYELVINSNPCIAYLME
                     ENTTAMQALVIAHACFGHNSFFKGNYLFRTWTDASAIVNYLLFAKNYIAECEERYGLD
                     ATESLLDSCHALMNYGVDRFRRPPHISADDERRRQIEREDFLQQQVNDLWRTLPKKDM
                     PKDEPWPRFPDAPQENLLYFIEKNAPLLEPWEREVVRIVRKIAQYFYPQRQTKVMNEG
                     WATFWHYVMLNTLYDEGLLTDGFMLEILQSHTNVTCQPEFDSPHFSGINPYALGYAIF
                     SDIRRICESPTDEDRAFFSELVGKDWLESVHHGMQNFKDESFILQFLSPKVIRDLKLF
                     SILDDDQEKEIQVTAIHDDLGYRRIREELADQYNLSKYEPNIQVYEVDLRGNRSITLR
                     HIQQDRIPLQKDDATAVMKHLHALWRFDVQLESIQDGRVTATYLCDNQSVRRIANEAA
                     A"
BASE COUNT         1643 a         2128 c         2088 g         1325 t
ORIGIN      
        1 gtcgtcgagg tgctgctcag gaaaagcgac cggctatatc tcaatgacgg catgtacggc
       61 atcttctggg aactgaggtt ccgcggccac gaccgctatc cggtcaaggc atatcgcgac
      121 ggcgcggagc tacaaggagc actccggagc tacaagctct acggccccac ctgcgacggc
      181 agcgatatgc tgccggggca agtggacctg cccgcggaca cgcgtccggg cgattacctc
      241 gagttcggcc gcatcggcgc ctacagtctt tccgggcgca ccaacttcaa cgggcactac
      301 agcgacactg tcgtgaccat cgaggcaggg acgcctccgg gctagcctcg cccatcctcc
      361 gcggaggttt ttcctcgaca gcgtgaacag ggtcactaat ccaggtgcca cgcctgctcg
      421 ctagaaaacc gcgcagccag gaaatccacg aaggtgcgca ccttggccga aagctgccgg
      481 cggctcgggt agagcgcggc gatcggcgcg cgcggcaaac tgtagtccgc gagcagccgc
      541 acgagttccc cggacttcac ctccggtgaa acgatgaaat cgggctcgat gatcacgccc
      601 aggtcagccg cggccatcgc agcgagcatg cgcccgttgt tggcgcgatg gcgcggcgaa
      661 acggacagcg catagctttc gccttgcgaa tcctcgaagc gccactcatt tggcacgggt
      721 acgagcgtat atgacaggca gtcgtgctcc gcgagttctt ccggtgtctg cggcgtgccg
      781 cgtcccgcca ggtacgcggg cgacgcgcaa cacacgatct gcgtccagcc gagccgccgc
      841 gacaccagcc cctgctgcga aactgaaccg atacgaatcg ccatgtcgaa gccttcctcg
      901 acgagatcca tcgggcggtc cgaaaggtcc aggtcgaaga cgacctgctg gtgctgcgcc
      961 gcgaactccc cgatggcagg cgcgatgaag cgttcaccga agctcacgcc gcaggtgagc
     1021 ttcaaggttc ctttcggcgt gactgcggca gcgcgcatcg caagctctgc ttcctcgaga
     1081 tcggaaagca actgcacgct gcgctcgtag aacgagcgcc ctgcctcggt cagtgaaagc
     1141 cgccgggtgg tgcggttgag gaggcgcgct tcgaggtgcg cctcgaggtc cgagaccttg
     1201 cgagagacgg ccgatgtcga caggtcgagt cgctctgccg cgcgagcaaa gctgcccgac
     1261 tcgacgaccc tggcaaacgt ctcgatggcg cgaaagcgat ccatggctga ttattgcata
     1321 gagtgcaaca gtgtagcaac gaggatgctg tttatccttg cagaagggat agccaaacta
     1381 gccaccatca ctcaccgaca ggagaaacgc aatgaagctc tattacaccc ccggcgcctg
     1441 ctcacaggcc ccccatatag ccttgcacga actgggcctg cctttcgaag ccgtccaggt
     1501 cgacctcgcc acccacaagt tgccggatgg cagcgattac tacgccgtga atcccaaggg
     1561 gtatgtgccg ttcctggtgc tcgaagatgg cacggacttg tcggaagccc acgtcatcct
     1621 gcagtacgtc gcggaccgcg aacccggcgc gctggcgccc gaattcggca cgatcgagcg
     1681 ctggaagctg atggaatggc ttggtttcgt cgccacagaa gtgcacaagg ggttcgggcc
     1741 gctctggtcg ccgcagacgc cgcccgaagt gcgcgagcgc acggtcagcg cactgggcaa
     1801 ccgcttcact gtgctcgccg aaacgctcgg caggcagcca tttcttacgg gcgagaagtt
     1861 cactatcgcg gacgcttact tgttcgtggt actgaactgg acgaaagtgc acaaagtcga
     1921 cctgaagccg tggccggccc tgtccgcttt ccaggcgcgc gtagccgccc ggccgtcagt
     1981 gcaggcaacg ctcgaggatg aaggactcat ggcccggaaa cgcccggccc gcgcggcagg
     2041 ttgacctcgc gcggaaatcg ctgaagcgcc tcccgcatcc cgcggcgcac tctcccggtg
     2101 cgaccgatgc gggtggcgca gggtaccggc aagcgaggac aatatgcctc gcttgcgctc
     2161 ccgcgcccgt caacagcgcc gtgatccgca tcatggctgc ttcaggggaa catcggggac
     2221 ttactttcct ccaattgtta ttatcagttc tcggatttca ttgtcggttt cgttgactta
     2281 gtcgacatta tcggcgtcga cttacggcaa gattcctgct aacaatgtga acacgtaaag
     2341 cacttttgca gcggacagta tcaagcgaat tctaaccatt cacaggcgaa agcccggggg
     2401 aatcggaatg agtatcttcg ccaactacca gaggcgttat gaagatacag aacaagaaga
     2461 attcagcctt caggaatacc tggagatttg caaaacagac aggacggctt acgcaagcgc
     2521 cgcagagcga ctgctcattg ccatcggtga gccgcaactc gtagacacat ccaaagatcc
     2581 gcgtctcagc cggatctttt ccaacaaggt catcaagcgc tacccggtgt tcgacgactt
     2641 ctacggcatg gaagaatgca tcgagcagat cgtgtcgttc ttcaagcatg ccgcgcaggg
     2701 actcgaagaa aagaaacaga ttctctacct gctggggccg gtcggcggtg gaaagtcttc
     2761 gctcgcggag aaactcaagc tgttgatgga ggtggttccc atctactcgc tgaagggttc
     2821 cccggtgcag gagtcgccgc tgggcctgtt caagccggcg gaagacgcgc ggatactccg
     2881 ggaggactac gggatacccg aacgctacgt caacaccatc atgtcgccgt gggccgccaa
     2941 gcggctcaag gaattcaacg gcgatatcac gaagttccgg gtggtcaaac ggcacccgtc
     3001 catcctgaac cagatcgcga tatcgaagac agagccgggc gacgaaaaca accaggacat
     3061 ttcctcgctg gtcggcaaag tcgatatccg caagctggaa gagtttccgc agaacgaccc
     3121 ggacgcctac agcttctccg gcggcctctg ccgcgccaac cagggcatcc tcgagttcgt
     3181 ggagatgttc aaggcgccga tcaaagtgct gcatccattg ctgactgcat cgcaggaagc
     3241 aaactacaac ggcaccgaat ctgtcggctc cattccgttc gacggcatta tcctggcgca
     3301 ctccaacgag gccgaatggc aggctttcaa gaacaacagg aacaacgagg ccttcatcga
     3361 tcgcgtctac atcgtcaagg ttccgtactg cctccaggtt accgaggaag tgaagatcta
     3421 tgaaaagctt cttcgtaaca gctcgttgag cgaagcgccc tgcgcgcctg atacgctgag
     3481 aatgctggcc cagttttccg tgctgacaag gctcgtcgaa ccggagaact ccagtatcta
     3541 ttccaagatg cgggtctacg acggcgagaa cctgaaggat atcgacccga aagccaagtc
     3601 gatccaggag tatcgcgaca ccgccggagt ggatgaagga atggagggcc tctcgacacg
     3661 gttcgccttc aagatcctct cgaaggtctt caatttcgac tctacggaaa ttgcggccaa
     3721 tcccgtgcac ctcctctatg tgctcgagaa gcaaatcgag cacgaacagt tcccggcaga
     3781 cgttcgcgac cgttacctga atttcatcaa ggaattcctc gcaccacgct acatcgcgtt
     3841 catcgggaag gagatccaga cggcgtacct ggagtcctac tcgcagtacg gccagaacat
     3901 cttcgaccgc tacattacct acgcagacct ctggatccag gaccaggact atcgcaaccc
     3961 ggacacgggg gagaacctcg atcattcggc tctgaatgac gaactcgaga aaatcgagaa
     4021 accggccggc atcagcaacc ccaaggactt ccggcacgag gtggtcaatt tcgtcctgcg
     4081 ggcacgggca gccaatcacg gcaagaaccc ggcgtggacc agctacgaaa agctcaggct
     4141 ggtcatcgag aaaaacatgt tttcgagcac cgaagacctg ttgccggtca tctccttcaa
     4201 tccgaaggcg tccacggaag acaagaagaa gcacaaggat ttcgtgagcc ggatgaccga
     4261 gtacggctat acggagaagc aggtaaggct gttatcggag tggtacctgc gcgtccgcaa
     4321 gtcgcggtga cctggtggca ttcctgatca tcgaccggcg ggaagagggt cgcaacaaga
     4381 gtgccgtcaa ccggcagcgc ttcctgcgcc ggtttcgcaa acacatcaag gaagctgtcg
     4441 ctgacgccgt cgacaaacgc agcatcaccg atatcgatcg cggcgatgag atcagtattc
     4501 cgaggaagga cgtatcggaa ccggttttcc ggcacggcag cggcggccgc cagcggcgca
     4561 tcctgcccgg taaccgggag ttcatcgccg gagacaggat caaacggcct gtgggcgggc
     4621 agggcggcgg aggtggccag gccagcaaca ccggcgaggg cctggatgat tttgccttcg
     4681 agctttcccg ccaggagttc ctcgatttca tgttcgagga cctcgcactc ccgaacctgg
     4741 tcaagaagca gctcaaggac acggagactt tccgctacca gaaaggcggt tatacgaacg
     4801 tcggcatacc ggcgagactg aacgtcgttc aatcgttgcg caatgcgcac gcgcgccgca
     4861 tagcgctgac gggggcaacg aaacgccgca tggatgcgct gcgggaagaa ctggaaggac
     4921 ttcaggatcg agcgggcgcg gatcagcgga tggaggagat caggctggaa ctggaacgtc
     4981 tcgagcggcg cctgaatcag attccgttcc tggacgactt cgacctcaag tacaacaacc
     5041 tcatccagca gccgctgcct acgagcaacg cggtgatgtt ctgcctgatg gacgtctccg
     5101 gctcaatgac ccaggaagtc aaggatgtag ccaagcgctt tttcatcctt ttgcacctgt
     5161 tccttaagcg agcctacaag aagatcgacg ttgtattcgt tcggcatcac actgtcgcaa
     5221 gcgaggtcga tgaggacgag tttttccatt cgagggagac cggcggcacg gtcgtatcga
     5281 gcgtgctgaa gctcatgctc gagatcatcc gcgaacgcta ccccagcgcc gactggaata
     5341 tctatgcggc acaggcatcc gacggcgaga actggccgga cgattcaccg attgcacgcg
     5401 atctcctgct caacggaatt ttgcccctgg tgcagtacta cgcctacgtg gaaattacaa
     5461 agtacgacca ccaggagctg tggcacgctt acgaggcggt tcaggagaag aaacctgact
     5521 gctttgcgat gaaaaaggtc acagacgcct ctgacatctt tccggtcttc cacgagctgt
     5581 tcaagaaata ggcgcaatgg tcgaacgacg ccccattgcc gaaacatcag agtggacctt
     5641 cgaaaatctt gccgatttcg aacgtgaact cgcccgtatt gcgaagcagt accggctgga
     5701 tacctattcg aaccagatcg agatcattac cgcggaacag atgatggacc ggtactcgtc
     5761 ggtcggcatg ccgctcggct accatcactg gtcgctggga aagcaattcc tcaacaccga
     5821 gcaaaagtac cggcgcggcc acatgggcct cgcctatgaa ctggtcatca attcgaatcc
     5881 ctgcatcgct tacctgatgg aggagaacac gacggcgatg caggcactcg tgattgcaca
     5941 cgcgtgcttc gggcacaact cgttcttcaa gggaaactac ctgttcagaa cctggaccga
     6001 tgcgagcgca atagtcaact acctgctgtt tgccaagaac tacatcgccg aatgcgagga
     6061 gcgctatgga ctcgatgcga ccgaatccct gctggactcc tgtcatgcgc tgatgaacta
     6121 cggagtcgac cgttttcgcc ggccgccgca catttcggct gatgacgaga ggcggcgcca
     6181 gatcgagcgg gaggatttcc tgcagcaaca ggtgaatgac ctgtggcgca cgctgcccaa
     6241 gaaggacatg ccgaaggacg aaccatggcc gcggttcccg gacgcgcccc aggagaatct
     6301 cctgtacttc atcgaaaaga atgccccgtt gctggaacca tgggaacggg aagtcgtcag
     6361 gatagtccgg aagatcgcgc agtactttta cccgcaacgc cagacgaagg tcatgaacga
     6421 aggctgggcg acgttctggc actacgtgat gctgaacacg ctatacgacg aaggcctgct
     6481 gaccgacggc tttatgctgg agatcctgca gtcgcatacg aacgttacct gccagcccga
     6541 atttgactca ccgcatttca gcggcatcaa tccttacgcg cttggttatg cgattttttc
     6601 cgacatccgc cggatctgcg aatcgcccac ggatgaggat cgcgctttct tttccgaact
     6661 cgtcggcaag gactggctgg agtcggttca ccatggaatg cagaacttca aggatgaaag
     6721 tttcatcctg cagttccttt cgccgaaggt catacgtgac ctgaagcttt tcagcatcct
     6781 cgacgacgac caggaaaagg aaatccaggt gacggcgatt cacgatgacc tgggttaccg
     6841 ccggattcgc gaggagcttg cggatcagta caacctcagc aagtacgagc cgaacatcca
     6901 ggtctacgaa gtcgacctcc gcgggaatcg ttcgatcacg ttgaggcaca tacagcagga
     6961 ccgcataccc ctgcaaaagg acgacgcaac ggccgtgatg aagcacttgc atgccctgtg
     7021 gcgttttgac gttcagctcg aatcgataca ggacgggcgg gtgaccgcga cctacctctg
     7081 cgacaaccag tccgtgcgga ggattgcgaa cgaagcggca gcctgaacgg tttaggtggc
     7141 aggatgatcg acaaactcat tgaagcgttg ccgaaagcgg agtt
//
LOCUS       DASUOM010000030         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_830654,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000030 DASUOM010000000
VERSION     DASUOM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830654"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /locus_tag="VFY27_00675"
     CDS             <1..2545
                     /locus_tag="VFY27_00675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5764054.1"
                     /translation="LWQPGTDHAGIATQMVVERQLVAEGKSRHGLGREAFNARVWRWK
                     EESGGTIARQLRRLGASVDWSRDRFTMDPAMSRAVQHAFIRLHADGLIYRGKRLVNWD
                     PVLHTALSDLEVLQSEESGQLWHFRYPLLDGSGHVIVATTRPETMLGDSAIAVHPDDD
                     RYRELVGREVVLPLVERRIPVVADDYVDPQFGTGCVKITPAHDFNDYELGLRHALAIY
                     NVLDDDAAMNESVPPAYRGLDRFAARRKVVEDMQALGLVEKVEDHEMSVPRGDRSGAI
                     VEPYLTDQWYVDIKPLARPAIEAVESGRIRFVPEGWTKTYYDWMHNIEDWCVSRQLWW
                     GHRIPAWYDDAGEVYVARTERAARDKYGLPDNLPLRQDEDVLDTWFSSGLWPFSTLGW
                     PAETEDLRTFYPGNVLVTGFDIIFFWVARMIMLGLKFMGDVPFREVYIHGLIRDQDGQ
                     KMSKSKGNVLDPLDLIDGVDLDTLLAKRTAGLMQPHLKPKIDAATRKQFPKGIPAFGA
                     DALRFTFAALATTGRDIRFDLGRIDGYHKFCNKLWNASLYVVMNTEDFLRGDVRLSPA
                     DRWIRSRLNRTILSVHKNLADYRLDLAAQALYDFTWHEFCDWYLELSKPVLQSDTSSD
                     EERRAAQQTLLDVLEMQLRLLHPLMPFITEEIWLNVGPRARAEGETVMLQPFPQFRED
                     CHDQDAEDEIAWVMQFILGIRQIRGEMDISPGKPLPVLLQGADAMDVELAGRHRRLLE
                     RVGRVESVRALGEGEVAPMSATALPGKMKLLVPMKGNIDVAAEKSRLTRQRQKLEAEI
                     EKSRQKLGNANFVNNAPAAVVTMETERSAEFARQLGQLNEQLARLAAVE"
     gene            2856..>3346
                     /locus_tag="VFY27_00680"
     CDS             2856..>3346
                     /locus_tag="VFY27_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5764055.1"
                     /translation="MAAIAATREALGNIILGKDVEISMALACLLARGHLLIEDLPGLG
                     KTMLAQALARVLGLSYQRIQFTSDLLPADIVGVSIFRQQPGEFVFPPGPVFAQLVLAD
                     EVNRATPKAQSALLEAMEEQQVSVDGQTHRLPQPFFVVATQNPSDQVGTFPLPESQLD
                     RFL"
BASE COUNT          641 a         1045 c         1066 g          594 t
ORIGIN      
        1 gctgtggcag ccgggaacgg accacgcggg catcgcgacg cagatggtcg tggagcgcca
       61 gctcgtcgcc gagggcaagt cgcgccacgg actcgggcgc gaggccttca atgcgcgggt
      121 ctggcgctgg aaggaggaat ccggcgggac gatcgcgcgc cagttgcgcc gtctcggcgc
      181 ctcggtcgac tggagccgcg accgcttcac gatggatccc gccatgtcgc gcgccgtgca
      241 gcatgcgttc atccggctgc acgcggacgg cctgatctac cgcggcaagc gcctcgtcaa
      301 ctgggatccg gttttgcaca cggcactgtc ggatctggaa gtgctgcaat cggaagagtc
      361 ggggcagctg tggcacttcc gctacccgct cctggacggc agcggccatg tcatcgtggc
      421 gaccacgcgc cccgagacca tgctgggcga cagcgccatc gccgtgcatc ccgacgacga
      481 ccgctatcgc gagctggtcg gcagggaagt cgtcctgcca ctcgtcgagc gcaggatccc
      541 ggtggtcgca gacgactacg tcgacccgca attcggcacc ggttgcgtca agatcacgcc
      601 ggcgcacgac ttcaacgact acgaactcgg gctgcgtcac gctctcgcga tctacaacgt
      661 tctcgacgac gatgccgcga tgaacgaatc cgtccctccc gcgtatcgcg gcctggatcg
      721 cttcgcggca cgcaggaaag tcgtggaaga catgcaggca ctgggcctcg tcgagaaggt
      781 cgaggatcac gaaatgagcg tgccgcgcgg cgaccgcagc ggcgcgatcg tcgaaccgta
      841 cctgaccgac cagtggtacg tggatatcaa gcctcttgcg cgtccggcga tcgaggctgt
      901 cgagtcgggg cggatccgtt tcgtccccga aggctggacc aaaacctatt acgactggat
      961 gcacaacatc gaggattggt gtgtcagtcg gcagctgtgg tgggggcaca ggatcccggc
     1021 gtggtacgac gatgccgggg aggtttacgt cgcacgcacc gaacgcgcag ccagggacaa
     1081 gtacggcctt cccgacaacc tgccgctcag gcaggacgag gacgtcctgg acacgtggtt
     1141 ttcctcgggt ctctggccgt tcagcaccct gggctggcct gcggaaacgg aagaccttcg
     1201 cacgttctat ccgggcaacg tgctcgtcac cggcttcgac atcatcttct tctgggtcgc
     1261 caggatgatt atgctcggct tgaagttcat gggcgacgtg ccgttccgcg aagtctacat
     1321 tcacgggctg atccgggacc aggacgggca gaagatgtcc aagtcgaaag gaaacgtgct
     1381 cgaccctctc gacctgatcg acggcgtcga tctcgacact ctcctggcga agcgcacggc
     1441 cgggctcatg cagccgcacc tgaagcctaa aattgacgcc gcaacgcgca agcagtttcc
     1501 gaagggtata cccgcattcg gcgccgatgc gctccggttc acgttcgccg cgctcgcgac
     1561 aacaggacgc gacatccgtt tcgatctcgg ccgtatcgac ggttaccaca agttctgcaa
     1621 caagttgtgg aacgcatctc tttacgtcgt gatgaacacg gaggatttcc tgcgcggcga
     1681 tgtacggctg tcgcccgccg accggtggat ccgctcgcgg ctcaacagga cgatcctgtc
     1741 cgttcacaaa aacctcgccg actacaggct cgacctggcc gctcaggcgc tctacgactt
     1801 cacgtggcac gagttctgcg actggtacct cgaactttcg aaaccggttc tgcaatcgga
     1861 tacctcgagc gacgaagaga ggcgagccgc gcagcaaact ctcctcgacg tgctggagat
     1921 gcagttgcgc ctgctgcacc cgctgatgcc ctttatcacg gaggagatct ggctgaacgt
     1981 cggtccgcgg gcgagagccg agggcgaaac cgtcatgctg cagccgtttc cgcagttccg
     2041 ggaggactgc cacgaccagg acgccgagga tgaaatcgcc tgggtcatgc aattcatact
     2101 cggtattcgg cagatccgcg gcgaaatgga catatccccc ggtaagcccc ttcccgtact
     2161 gttgcagggg gcagacgcga tggatgtcga actcgcagga cgacatcggc gcctgctgga
     2221 gagagtgggc cgcgtcgaat cggtgcgtgc gctcggcgaa ggggaagtcg cgccgatgtc
     2281 cgcgaccgca cttcctggaa agatgaaact actggtgccg atgaagggca atatcgacgt
     2341 agcggccgag aaatcccggc tgactcgcca acgtcagaaa ctggaggcgg agattgagaa
     2401 atcccggcaa aagctcggga atgcgaattt cgtgaacaat gcgccggccg cagtagtcac
     2461 aatggagacc gagcgctcgg cggaattcgc gcggcagctg ggccagctga atgagcagct
     2521 cgcgaggctc gccgctgtag agtagcggac acaccttcgc gatggtgtac agccgcacaa
     2581 ttgcgcggtg ggctattggc aactgtagga gcccgccatg cgggggtttc gacgagccag
     2641 atcggactcg cccgcaaggc gggctcctac aggttgcacc ggtcggacca ggtccaatct
     2701 gcgcactggg tcacaatctg cgcgcgcctc gtgtgcattg gcgttcttca tccgcgacca
     2761 tggatgctgt tttagacggc taacgggagt atccgatgca gcggggaaat acgctggagc
     2821 agctgccgct gaatgcggaa ggccacaagg ctgccatggc cgcgattgcc gcaacccggg
     2881 aagcgctcgg gaacattatc ctcggaaagg acgtggagat ttcgatggcg ctcgcctgcc
     2941 tgctggcgcg cggacatctc ctgatcgagg acctgccggg cctcgggaag acgatgctcg
     3001 cacaggcact cgcgagggtc ctgggtttga gttaccagcg catccagttc accagcgacc
     3061 tgcttcccgc cgacatcgtt ggcgtttcca tctttcgcca gcaaccgggc gagttcgttt
     3121 tcccgccggg tccggtgttc gcgcaactcg tgctcgcgga cgaggtcaat cgcgctacac
     3181 ccaaggcaca gagcgccctg ctcgaagcga tggaagaaca acaggtttcc gtcgacgggc
     3241 agacgcaccg gctgccgcag ccgttcttcg tcgtcgcgac gcagaatccc tccgaccagg
     3301 tcggtacctt tcccttgccg gaatcccagc tcgatcgctt cctgat
//
LOCUS       DASUOM010000031         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_108304,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000031 DASUOM010000000
VERSION     DASUOM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108304"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..308
                     /locus_tag="VFY27_00685"
                     /pseudo
     CDS             165..308
                     /locus_tag="VFY27_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(348..1576)
                     /locus_tag="VFY27_00690"
     CDS             complement(join(348..1288,1288..1576))
                     /locus_tag="VFY27_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611960.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX5764056.1"
                     /translation="MSKTTNKFSAEVRERAVRLVLDHEKDHPSRWAAVSSVASKIGCS
                     AHTLNEWVKKAEVESGSRAGVPLDVLEKLKAQEREIRELRQANEILRKASAYFCDGGA
                     RPPTEMMLGFIEAHRQEHGVEPICKVLPIAPSTWRRHAARAADPAKLPARAQRDAGLK
                     ADIRRVFEENFQVYGVRKVWRQLQREGIQVARCTVARLMREMGLRGAIRGKPAKTTIA
                     DKGLPCPLDRVNRQFRADRPDQLWLSDFTYVSTWSGFVYVAFIIDAFARRIVGWRVSR
                     TADAGFVLDALEQALHQRRPAGKSGLVHHSDRGSQYLSIRYTERLGLAGIEPSVGSVG
                     DSYDNALAETVNGLYKAELIHRRGPWKSFEAVEYATLEWVDWFNNRRLLEPIGNIPPA
                     EAEQRYHDAVNAQPLAA"
     misc_feature    complement(1179..1295)
                     /locus_tag="VFY27_00690"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1717..1956)
                     /locus_tag="VFY27_00695"
                     /pseudo
     CDS             complement(1717..1956)
                     /locus_tag="VFY27_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2188..>2569
                     /locus_tag="VFY27_00700"
     CDS             2188..>2569
                     /locus_tag="VFY27_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764057.1"
                     /translation="MQKPAEHQADMPRVQRFLFWLFALCFVLVGILLTPELSGRMMAE
                     AGYLPDRSWKYMRLVSSAALAIGLLLAVTPLLPSRHGRRWLGALSYVGALLLIFELSS
                     AVLLRLTFGFNHIRDCTQYSFHPSL"
BASE COUNT          424 a          868 c          743 g          534 t
ORIGIN      
        1 gacgccgacc gcgtgccctg gctccccgat ctcatccgcc aaaacgcctc agcgtagtcc
       61 agctcagatc atcatgaccg ctagcagcct gtcggactta acggcatagg gcgggcctga
      121 tagaatcagg gcatgtttcg agagcttttt caggctattt ctcgatgagc aacttccgcc
      181 ctgtcgatcg tgacaccggt ttcctgctgc cgccgtcggt tgacgagtgg ctgccggaga
      241 agcacttggc gcgcttcgta gtggaggtga tcgacggcct cgacctgggg cggatggccc
      301 gggcctactg aaccgcgccg gaattgtcgg cggcaaaagt tcgtgagtca tgccgccagg
      361 ggctgagcgt tcacggcgtc atgatagcgt tgttcggcct cggccggtgg gatatttccg
      421 atgggctcga gcaggcgcct attgttgaac cagtcgaccc attcgagggt ggcgtattca
      481 acggcttcaa aggatttcca tggtcctcgc ctgtggatga gttcggcctt gtagagaccg
      541 ttgacggttt cggccaaggc gttgtcgtag ctgtccccga cactgccaac cgacggttcg
      601 atgccggcca ggccgaggcg ctcggtatag cggatggaca ggtattggct tcccctgtcc
      661 gagtgatgga ccagtccgga tttcccggct ggccggcgct ggtgcagggc ctgctccagg
      721 gcatccagga caaagccggc atcggccgtc cgggacaccc gccagccgac gatccgccgg
      781 gcaaaggcgt cgatgatgaa agccacatag acaaagccgc tccaggtcga gacgtaggtg
      841 aaatcggaaa gccacagctg gtcgggccta tcggcccgga actgccggtt cacccggtcc
      901 agcggacagg gcaaaccctt gtcggcgatg gtggttttcg ccggcttgcc gcggatggca
      961 ccgcgcaggc ccatctcccg catcagccgc gcgaccgtgc agcgcgccac ctggatgccc
     1021 tcacgttgca gctgccgcca caccttgcgc accccataga cctggaaatt ctcctcaaac
     1081 acccgccgga tgtcggcctt caagccggca tcgcgttgtg cccgcgccgg cagcttggcc
     1141 ggatcggccg cgcgcgccgc atgccgccgc caggtcgatg gggcgatcgg cagcaccttg
     1201 cagatcggct cgaccccgtg ttcctggcgg tgggcctcga tgaaacccag catcatttcg
     1261 gtcggcggtc gagctccgcc atcgcaaaat aagccgaggc cttgcgcagg atctcgttcg
     1321 cctggcgcag ctcccggatt tcccgctcct gcgccttcag cttctccagc acatccagcg
     1381 gaacgccggc gcggctgccg ctctccacct cggctttctt cacccattcg ttcagcgtat
     1441 gcgccgaaca gccgatcttc gacgccacgg acgaaaccgc cgcccatcgg gacgggtgat
     1501 ccttctcgtg gtccagaacc agccgcacag cccgctcgcg gacctcagcc gaaaacttgt
     1561 tcgttgtctt gctcatcgtc gctccatcct ctcaggagtt ggagccgccg acaatcccgg
     1621 agcggttcag gccgctcccc tccaccgtcc atggtaacgc caaccgtttc tccgcccaca
     1681 ggcggaactg cggtccgcga tggtttttta agatatcatt ttcacgcgcg acaattctgt
     1741 tcgcagacga ctgatttcca tttgttccgc gctgactgcc gatgtagcag aacttttcaa
     1801 tccgccgttg cccgccgcct cgacccagtt atgcagcgtc tgctccgaca ggccgagatt
     1861 cctggcgacc gtcgccaccc gctcgccacc ggtgaccaga cgaacggcct cttccttgaa
     1921 ctccagcgtg tagcgcgccc gtgttgattt cgtcattccg cctccatcct ctcgccgatc
     1981 ttacccggct atgggaggcg ctgcacgggg gcaaggtcag tttgcttcgc aacaatcaaa
     2041 ccacgctgtc cacatcccct caccagcccg cccgaagcac ccccattgcc cggttggaca
     2101 gccggctatc cgtcccttat tcggatcacc gatccgggta cgggttcgga gcagcgaccg
     2161 tgtgcctatt cttcctctct catcggcttg caaaagccag cggagcatca agccgatatg
     2221 ccccgagttc aacgattcct cttctggctt ttcgccctgt gcttcgttct tgtcggcatc
     2281 ctgctgaccc cggaactcag tggccgtatg atggccgaag ccggatatct gcccgaccgc
     2341 agttggaaat atatgcggct ggtatcgtcg gcggccctgg cgatcggcct gctgctggcg
     2401 gtcacacctc tgctgccgtc gcggcacggc cggcgttggc tgggcgcctt gtcctatgtc
     2461 ggggcgcttc tgctgatctt tgaactgtcc tcggccgtgt tgctgcggct cacctttggg
     2521 ttcaatcaca tcagggactg cacccagtac agctttcatc cctcgctga
//
LOCUS       DASUOM010000032         5927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1309612,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000032 DASUOM010000000
VERSION     DASUOM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309612"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2244
                     /locus_tag="VFY27_00705"
     CDS             <1..2244
                     /locus_tag="VFY27_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5764058.1"
                     /translation="IYFDQKRAGENFSDLYVVPAAGGEPRQVAPADESKSSNASRVYN
                     AARTMVAWIHRGDIMVKDLSSGQTRQLTRTTAEETSPMFMADGTSVAFVRDKLHFVHD
                     PAAGTTAEAADIRFEKHPDEQSFDTLREQQMRNLETLREDKRRAEALEADAEARQHED
                     PAGAPLPIYLGEDYTEVGRSLSPSGRHLLVTVTDDEVDEGRAGMMPNYVTEDGYTSIL
                     ETRTRVGRNEPAAQQLLLVDLLEGRFETVDYASLPGLDEDPLQELRESAVDWHVGRGA
                     DREEVEKALEAPETRTLTVERVVWNNPGSLAAVQLRSVDNKDRWLATIDPREPLVTPQ
                     HRLTDEAWINWDYNEFGFMPDGRTLWYLSEESGFSHLYTKRVNSRRTRQLTSGEFIVR
                     EPELNPQGSHFYVVTNRTHPGNYEVYRVAVDGGDLEQVTELGGVVTSRLSPGGDELLL
                     SRSSLDRHADLYIQPATAGGEAVKLTDTVSERFKDIDWVIPEILPVPSSQVDKPIYTK
                     LYLPPDYREALSYPAVMFVHGAGYTQNSHMGWPYYFREFMFHTILANEGFVVIDMDYR
                     ASQGYGRDWRTAIYRNMGRPELEDFKDGIDWLAENYGVDRSRVGIYGGSYGGFMTFMS
                     LFLEPDLFAAGAALRPVADWMHYNHTYTSNILNTPVIDPEAYERSSPINYVQNLEKPL
                     LIASGMQDDNVFFQDSVLVVQRLIELKKENFEFAVYPLDPHGFVHPNSWLDEYRRIYK
                     LMNEHLK"
     gene            complement(2376..3083)
                     /locus_tag="VFY27_00710"
     CDS             complement(2376..3083)
                     /locus_tag="VFY27_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5764059.1"
                     /translation="MGAADGQIRFLTWNVHSCIGTDRRFDPERVKSIIKAIDPDIAAL
                     QEVDSRRDLRDGFDLLGNTLGSHSAEVRTVRTPDRDYGHMLLSRWKIAAWTHHDLSFR
                     NREPRSLIEASIETDRGAVSVLAAHLGLSHGERRQQAQTIAALAEADGLPTIILGDFN
                     EPTGNGTAGRMLGRLFHSIGRHATFPSRWPFLPLDRIWFESSLSLIQAGVHREAPTAS
                     DHLPLWADFRLRTGRKI"
     gene            complement(3086..5218)
                     /locus_tag="VFY27_00715"
     CDS             complement(3086..5218)
                     /locus_tag="VFY27_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5764060.1"
                     /translation="MAETLRRDVLEEGRTVWKLTHADRAAVLVDAADYFGALRAAMGA
                     ATRSITVVGWDIDSRTPLVGRSGEAPDGAPATLLAFIEALVEKRPLLDVRLLLWDYTI
                     LYALDREPLPSLNLKWRTPPQVKVELDSCLPLGACHHQKLVIVDGNLAFCGGLDLTTR
                     RWDTPEHRPRHPFRRDADDKPYPPFHDIQMLVDGEAAKHLAGIASRRWREAAGEKISV
                     VETEVRWPENVAPDCQDVEVGIAQTLPATDEREAVKQVYELYLATIRTARRYIYIENQ
                     YLTSDEIAEALAARVREIPELEVLVITPWAPEGWLEKRTMGAGQQRVMQKLSQEGLGA
                     RVKFLYPWVGSAREQTPVMVHAKLMIVDDLFLRVGSSNLNNRSMGVDTECDLLLRAST
                     DGHRAGIRSVLHRLLAEHLGLDVSEVADKWQESGSLLKLAQGKWCDERGLKPLRQHAK
                     YQALSETLNLVADPEVPLEPSEFVGDMFGAAGGNRYATRRVLKLAAIAAMLLLLLLAW
                     SYTPLKDFADPEAVADALYRVRSEWWIYPAVLAAFVFGGILLFPVTVMIAVSGMLLGP
                     WTGWFYAMLGSMLSAWVGHATGRWLGGSPLRNISGRAFRAVSQALKNQGVIAVAALRM
                     VPIAPYTVVNMAMGAAGVSARVFLAGSFLGMLPGTFVLTMLGDRLREAWQEPAPANLV
                     LFALVVVLWLGLAFVLQQVVSRLKKRER"
     gene            5336..5773
                     /locus_tag="VFY27_00720"
     CDS             5336..5773
                     /locus_tag="VFY27_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764061.1"
                     /translation="MIQAHVKKQFDAAIENVESALRDLSDRLESLSQSRRPPTAMRRA
                     GRNLKRRASSFADHVPLERASAIAADTGRTVRQHPVTTVLTAAVAGYCIWSLVQYSTN
                     RTAAGRKSSNRRARDNFFGEGRTGEDSPQRVVLPGEPDSAPRH"
BASE COUNT         1166 a         1897 c         1835 g         1029 t
ORIGIN      
        1 atctacttcg accagaagag agcgggtgaa aacttcagcg acctttacgt cgtaccggcc
       61 gcgggtggcg agccacggca ggttgcgccg gccgatgagt cgaaaagctc gaacgcatca
      121 cgtgtgtata acgccgcgcg cacgatggtc gcctggatcc atcgcggcga tatcatggtc
      181 aaagacctgt ccagcgggca gacaaggcag ctgacgcgta ccacggcgga agagacctcc
      241 ccgatgttca tggcggacgg tacgtcggtt gcgttcgtgc gagacaagct gcatttcgtg
      301 cacgaccccg cggcgggaac caccgcggaa gctgccgaca tccgtttcga aaagcacccg
      361 gacgagcagt cgttcgacac gctgcgcgag cagcagatgc gaaacctcga gacccttcgt
      421 gaagacaagc gccgcgcgga ggcgctcgaa gccgatgcag aggcccgtca gcacgaagat
      481 cccgccggcg caccgcttcc gatctacctg ggggaagact acaccgaagt cggccgctcg
      541 ctctcgccct ccggccggca cctcctggtg acggtgacag acgacgaggt cgacgaaggg
      601 cgggccggca tgatgccgaa ttacgtcacc gaggacggct acaccagcat cctggaaacc
      661 cgcacgcggg tcggcaggaa cgagccggcg gcacagcaat tgctgctggt cgatctcctc
      721 gagggcaggt tcgagacggt ggactacgcc tcgctgcccg gccttgacga agacccactg
      781 caggaactgc gggaaagcgc ggtcgactgg catgtcgggc gaggcgccga cagggaggaa
      841 gtcgagaagg ccctggaagc ccctgagacg cggacgctga ccgtcgaacg ggtcgtctgg
      901 aacaaccccg ggagtcttgc agcggtgcag cttcgctccg tcgacaacaa ggaccggtgg
      961 cttgcgacca tcgatccgcg cgagccgctg gtaacgccgc agcatcggct gacggacgag
     1021 gcctggatca actgggacta caacgagttc ggcttcatgc cggatggccg gacgctctgg
     1081 tacctctcgg aggaatcggg tttttcgcac ctttatacga agcgtgtgaa ctcccgcagg
     1141 acgcggcaac tgacgagcgg ggagttcatc gtccgcgagc cggaactcaa cccgcagggc
     1201 agccactttt acgtcgtgac gaaccgcacg caccccggca actacgaggt gtaccgggtt
     1261 gctgtggacg gcggcgatct cgaacaggta accgagctcg ggggtgtagt cacatcccgc
     1321 ctgtcacctg gcggtgacga gcttctgctg agccgctcgt cgctggaccg ccacgccgac
     1381 ctttacatcc agccagccac tgccggtggc gaggcagtca agctcacgga caccgtgagc
     1441 gagcggttca aggacatcga ctgggtaatt ccggagattc tcccggtgcc gtcctcgcag
     1501 gtcgacaagc cgatctacac gaagctgtac ctgcctcccg actatcgcga agccttaagt
     1561 tatcctgccg tgatgttcgt ccacggcgcc ggctatacgc agaactcgca catgggctgg
     1621 ccttactact tccgcgagtt catgtttcac acgatcctgg cgaacgaagg ttttgtcgtg
     1681 atcgacatgg attaccgtgc atcccagggc tacgggcgcg actggcgcac cgccatctat
     1741 cgcaacatgg ggcggcctga actcgaggat ttcaaggacg gcatcgactg gctggcggag
     1801 aattacggag tcgacaggag ccgcgtcggg atctacggcg gatcctacgg cggcttcatg
     1861 acgttcatgt ccctgttcct cgaaccggac ctgttcgcgg caggcgcagc gctgcggccg
     1921 gtggccgact ggatgcacta caaccacacc tacacctcga acatcctgaa cacgccggtg
     1981 atcgacccgg aagcctacga gcgcagctcc ccgatcaact atgtgcagaa cctcgagaag
     2041 ccgctcctga tcgcaagcgg catgcaggac gacaacgtgt tcttccagga ttcggtactg
     2101 gtcgtacagc gcctcatcga gctgaaaaag gaaaacttcg agttcgcggt gtacccgctg
     2161 gacccgcacg gcttcgtgca cccgaactca tggctcgacg agtaccggcg tatttacaag
     2221 ctgatgaacg aacatctcaa atagcatgca ggagcccacg ctaagcggaa tcggaaacaa
     2281 tatgaatccg gacggccgtt cggacggcga atgcgggcgc gttcgctgag gccaggtcga
     2341 actcgcccgc atggcgggct cctacagcgc tcgaatcaaa ttttcctgcc ggttcgcagg
     2401 cggaaatctg cccatagcgg gaggtggtcc gatgccgttg gtgcttcgcg atgcacgccc
     2461 gcctggatca gcgacaggct cgactcgaac cagattcggt cgagcggcag gaaaggccag
     2521 cgcgaaggaa aggtggcgtg acggccgatc gaatgaaaca acctgccaag catccggccg
     2581 gccgtaccgt tgcccgtcgg ctcgttgaag tcgcctagaa ttatggtcgg caagccgtcg
     2641 gcctccgcga gcgccgcaat cgtctgcgcc tgctgccggc gctccccgtg tgaaagcccg
     2701 aggtgcgcgg cgagaacgct caccgcgccc ctgtccgtct cgatactggc ttcgatcagc
     2761 gaacgcggct cgcgattgcg aaagctcagg tcgtggtggg tccatgcagc gatcttccag
     2821 cggctgagca gcatgtgccc gtagtcgcgg tcgggcgtac gcaccgtgcg gacctccgcg
     2881 ctgtgactgc cgagtgtatt tcccagcagg tcaaaaccgt ccctcaggtc acggcggctg
     2941 tccacctcct gcagcgcggc gatgtccggg tcgatggctt tgatgatcga cttcacgcgt
     3001 tccgggtcga agcgccggtc ggtgccgata cagctgtgca cattccaggt caggaagcgg
     3061 atctgcccgt cagccgcccc catgcttatc gctcgcgctt cttcagccgc gagacgacct
     3121 gttgcaggac gaaagcgagc ccgagccaga gtacgacgac cagggcgaac aacacgagat
     3181 tggcaggagc aggttcctgc catgcctcgc ggaggcggtc gccaagcatg gtcagcacaa
     3241 aagtgccggg cagcatgcct aagaaacttc ctgcaaggaa gacccgcgcc gagaccccgg
     3301 cggcgcccat tgccatgttc accaccgtgt acggggcaat cggcaccatt cgcagtgcgg
     3361 cgaccgcgat gacgccctgg tttttcaagg cttgcgatac cgcgcgaaat gcgcgtccgg
     3421 aaatatttcg cagcgggctg ccgccgagcc agcgcccggt cgcatggcca acccaggcgc
     3481 tcagcatcga gccgagcatc gcgtagaacc agcctgtcca cggtccgagc agcatgccac
     3541 tcaccgcgat catcacggta accggaaaca gcagaattcc tccgaacacg aaggcagcga
     3601 ggacggcagg ataaatccac cattcgctgc gtacacggta gagcgcgtcg gctaccgctt
     3661 ccggatcggc aaaatccttg agcggcgtgt agctccacgc gagcagcagc aggagcagca
     3721 tcgcggcaat ggcggcgagt ttcagcaccc gcctggtggc atagcgatta cctccggcgg
     3781 cgccgaacat gtcgccgacg aactcggacg gctccagcgg cacttcgggg tcggcgacca
     3841 ggttcaacgt ctccgacagc gcctggtatt tggcgtgctg ccggagcggc ttcaagccac
     3901 gttcgtcgca ccactttccc tgcgccagtt tgagtagcga accagactcc tgccacttgt
     3961 ccgcaacctc gcttacgtcc agtcccaggt gctcggcgag caaccgatgc agtaccgaac
     4021 ggatgcctgc ccgatgcccg tccgtcgaag cacgcagcag gaggtcgcat tcggtatcga
     4081 ctcccatgga gcgattgttc aggtttgacg agccaacgcg caggaagagg tcgtcgacga
     4141 tcatcagctt cgcgtgcacc attaccggcg tttgctctcg tgcggacccg acccacgggt
     4201 agaggaactt cacgcgagcg ccgagtcctt cctgtgacag cttctgcatt acgcgctgct
     4261 gccccgcacc catcgtgcgc ttctccagcc atccttccgg tgcccagggt gtgattacca
     4321 gcacctcgag ttccgggatt tcgcgaacgc gcgccgccag cgcctccgca atttcatcgg
     4381 aagtgaggta ctggttctcg atgtagatgt agcggcgcgc cgtgcgaatg gtggcgaggt
     4441 aaagctcgta tacctgcttt acagcttccc gctcgtccgt tgctggcaac gtctgcgcga
     4501 tgcccacctc gacatcctgg cagtcgggtg cgacgttctc cggccaccgc acctccgtct
     4561 cgacaacaga gattttctcg cccgctgctt cccgccacct gcgggatgcg attcctgcaa
     4621 ggtgcttcgc ggcttccccg tcgaccagca tctggatgtc atggaaaggc gggtacggtt
     4681 tgtcgtcggc atcgcggcgg aacggatggc ggggacggtg ttccggcgta tcccagcgcc
     4741 tggtggtcag atcgaggccg ccgcagaacg cgagattgcc gtccacgatc acgagcttct
     4801 ggtgatggca ggcgcccagc ggcaggcaac tgtccagctc gaccttcacc tgcggagggg
     4861 ttcgccactt caggttgagc gagggcaggg gctcccggtc gagtgcgtag agaatcgtgt
     4921 aatcccacag cagcaagcgc acatcgagta ggggccgctt ctcgaccagg gcctctatga
     4981 aagccagcaa ggttgccgga gcgccatccg gcgcctcgcc gctacggccg acaagcggag
     5041 tacggctgtc gatatcccag ccgacgaccg tgatcgaacg agtcgccgcg cccatggccg
     5101 cacggagtgc cccgaaataa tcggctgcgt cgaccagcac ggcggccctg tcggcgtgcg
     5161 tgagcttcca aaccgtgcgt ccctcttcca gcacgtcccg tcgcagagtt tcagccacgg
     5221 ggttgttcct ttttgttaat ggctcaccat cctggacctc ggcgctggca agagacttgc
     5281 atggcgagaa catcgccgct aaacacatcg tcaggaacga tatcaggagc ccccaatgat
     5341 acaggcacac gtgaaaaagc aatttgacgc cgcgatcgag aatgtggaat cggcgctgcg
     5401 ggatctttcc gaccggctcg agtcgctctc gcagtcgcgg cggccgccga ccgccatgcg
     5461 ccgtgccggt cgaaacctga aacggcgggc cagttctttt gccgaccatg tcccgctgga
     5521 gcgagcctcc gcaatcgccg cagacaccgg ccgcactgtg cgtcagcacc cggtgacgac
     5581 ggtattgact gccgcggtag caggctattg catctggtcg ctggtccagt actcgaccaa
     5641 ccggactgcc gccggcagga aatcgagcaa tcgccgcgcc cgcgacaact tcttcggcga
     5701 gggacgcacc ggcgaggact cgccgcagag ggtggtcctg ccgggggaac cagacagcgc
     5761 tccccggcac tagcgggcgc ccgacgacca aacggcataa attgcctgtt tttgcagaat
     5821 ctacatttcg cttctgcaac actgtctctt aatgacgacg tcggcgtgct ggtgcatggt
     5881 gcggcatgtg ttgccgacgt aaagcgttgt acggcaacga aaacagc
//
LOCUS       DASUOM010000033         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_590563,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000033 DASUOM010000000
VERSION     DASUOM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590563"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2160
                     /locus_tag="VFY27_00725"
     CDS             106..2160
                     /locus_tag="VFY27_00725"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5764062.1"
                     /translation="MTELSSNDTEAEGLTHWRVEQDSAGISWLCLDKADGSANVLSGP
                     VLRQLNDIIEPYRGAPPRGVVIYSGKPNGFIMGADINEFTRIKDPDEAYELIRLGQQV
                     LASLEALRCTTVAAINGFALGGGFELALACDYRLVLQTEKAIIGLPEVQLGIHPGFGG
                     TVRAVRVAGVRAAMGLMLTGQPIAPGRARRQNLVDRIVAPDNWKQAAKEMIATPQKRA
                     QPPLLDQALNLPLVRPFIARKMRVEVAKRARPDHYPAPYAIIDLWVQHGASEHHGYEA
                     EARSIAQLMCGETSRNLVRVFFLQNRLKSLGGKPAARIERVHVVGAGVMGGDIAAWCA
                     LRGLDVSLQDRAMEYIQPAMDRARKLFAKKVRTEYDQAATAERLKADPTGEGVADAGL
                     IIEAIFEDADAKKELYATLQKSMKADAILATNTSSIRLEDLREGLARPERFIGLHFFN
                     PVAKLPLVEVIRADDTAEEAMDIGFAFVKAIGKFPLECRSSPGFVVNRVLAPYMAEAM
                     LLAGEGVPLREIDRAAEDFGMPMGPIELLDSVGLDVALHVSNVLGKAFDKPVPSMLSQ
                     MVESKQLGRKSGRGFYAWEDGKAVKPEAEPGASVPPDLCDRLILVMVNEAVACLHENV
                     VSDADLLDAGVIFGTGFAPFRGGPLQYARSRGISDVQQRLRQLEEKYGKRFSPHAGWN
                     EL"
     gene            2467..>2677
                     /locus_tag="VFY27_00730"
     CDS             2467..>2677
                     /locus_tag="VFY27_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764063.1"
                     /translation="MKDEQLDTELLKSFSPLDGLKRDNLAALAKKVQIRELSPQQILF
                     KEGDTEKRTVYVVSGSLELIDQGQVQ"
BASE COUNT          569 a          766 c          854 g          488 t
ORIGIN      
        1 ggcattaccg tgacgggctt cgaggtgccg agcgtccgtc tttccgatca cctgccgctc
       61 atatgcgatt tcgacgtcga ctgagaacta ccccgggaac ccgaaatgac cgaactctcg
      121 agcaacgata ctgaagccga aggactgacc cactggcgcg tcgagcagga cagcgccggt
      181 atttcatggc tttgcctgga caaagccgat ggcagtgcca atgttttgtc cggacccgtg
      241 ctgcgccagc tgaatgacat catcgagccg taccgcggcg caccgccgcg cggcgtagtg
      301 atctactcgg gtaagccgaa cggcttcatc atgggcgcgg acatcaacga gtttacccgg
      361 atcaaggatc cggacgaagc atatgaactg atccgccttg gccagcaggt gcttgcctca
      421 ctggaagcgc tgcgttgcac gaccgttgcc gcgataaacg gttttgcgct gggcggcggg
      481 ttcgaacttg cgctcgcctg cgattaccgc ctggtcctgc agactgaaaa ggccatcatc
      541 gggttgcctg aagttcagct cggcatacat ccgggttttg gcggtaccgt gcgcgcggtg
      601 cgggtcgccg gggtgcgcgc tgccatgggg ctcatgctga ccggccagcc gattgccccc
      661 ggcagggcca ggcgccagaa cctcgtcgac cggatagtgg ccccggacaa ttggaagcag
      721 gcagcaaagg aaatgattgc gacgccgcaa aagcgcgcgc agccaccctt gctggaccag
      781 gcgctgaatc ttccactcgt acgtcctttc atcgcgcgca agatgcgcgt cgaagtggcg
      841 aagcgggccc gtcccgacca ctacccggca ccgtatgcga tcatcgatct gtgggtgcag
      901 cacggagcat cggaacacca cggatacgaa gcggaagccc gctccatcgc acagctgatg
      961 tgcggagaaa catcgagaaa cctcgtgcgg gtatttttcc tgcagaaccg gctcaagtcg
     1021 ctgggaggca aaccggctgc gcgtatcgaa cgtgtgcacg tcgtcggcgc aggagtcatg
     1081 ggtggagaca ttgctgcatg gtgtgcattg cgcggcctcg acgtcagcct gcaggatcgg
     1141 gctatggagt acatccagcc ggcaatggac cgggctagga agcttttcgc gaagaaggtc
     1201 cgtaccgaat acgaccaggc tgcgacagcg gaacggctca aggcagatcc gactggcgaa
     1261 ggtgttgccg acgcgggtct gataatagag gcaatattcg aagatgccga tgccaagaaa
     1321 gaactttacg cgactctcca gaagtcgatg aaggcggacg cgatcctcgc gactaacaca
     1381 tccagcatcc ggctcgaaga cctgcgcgag gggcttgcgc ggccggagcg gttcatcggc
     1441 ctgcatttct tcaatcccgt tgcgaagctg ccgctggtag aggtcatccg cgcggacgat
     1501 acggcagagg aggcgatgga catcggtttt gccttcgtca aggcaatcgg taagttcccg
     1561 ctggaatgca ggagctctcc gggtttcgtg gtcaatcggg tgctggcgcc gtacatggca
     1621 gaagcaatgt tacttgccgg cgaaggcgtt ccgctccgcg agatcgaccg cgcggcggaa
     1681 gatttcggga tgccgatggg ccccatcgaa cttctggaca gtgtaggtct cgacgtcgcc
     1741 ctgcacgtgt cgaacgtgct gggcaaggcg ttcgacaagc cggttccctc catgctctcg
     1801 caaatggtcg aaagcaagca gctcgggaga aagtctgggc gaggtttcta cgcctgggag
     1861 gacggcaagg ccgtgaaacc cgaagccgaa cccggcgcat cggtgccgcc ggatctctgc
     1921 gacaggctga tcctcgtgat ggtcaatgag gctgtcgcat gccttcacga gaacgtcgtc
     1981 agcgatgcgg acctgctcga cgccggtgtc attttcggca ccggattcgc gccattccgg
     2041 ggcgggccgc tgcagtatgc tcgcagccgc ggcataagtg atgtccagca acggcttcga
     2101 cagctcgagg aaaagtatgg gaagcgtttc agtccacatg cgggctggaa cgaactgtaa
     2161 accgaccggc gcgcgtcccc tcgacttgtt aaactgtgct ccgcttcacg gtgccgcata
     2221 gcggcggtat cggagcctga taaattgaag gtagaataac ggcttagccg gaggtacaag
     2281 cccggactgt tcatgaatcg ccgcgatgat tcggtgcgga caaggatcac gcaagggcgg
     2341 agcgtattca tgaatagtcc gggctggcaa ataccgcacg ccgcgcaggc gtgcaccggg
     2401 acgcaacagt ccgcaagcca gcggcctcaa cgcagcaaaa taaaatgcga gccaacagga
     2461 tgagacatga aggacgagca gctggatact gaactgctca agagcttctc cccgctggat
     2521 gggctgaagc gcgataacct cgctgcgctt gcgaagaaag tgcagattcg ggagttgtcg
     2581 ccgcagcaaa tcctgttcaa ggagggagat accgagaagc gcaccgttta tgtagtgtcc
     2641 ggttcgctcg agctgatcga ccagggacag gttcagg
//
LOCUS       DASUOM010000034         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_951223,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000034 DASUOM010000000
VERSION     DASUOM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951223"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VFY27_00735"
     CDS             complement(<1..1306)
                     /locus_tag="VFY27_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5764064.1"
                     /translation="MNKIRPQVAGVYGRLWKYVLPYRAIGAIAVIGMAATAAVEAGMV
                     SLLQPLTDDALVARNLETVRWMPVAFIAIFIARGLAGFATEMSLGWIGRGVISSLRRD
                     VFQKFLTLPSRFFENHATGPLLSRMTYNVEMVAESVTSVVTILIRDLLTIIAAVAVML
                     YQSVRLTIFVAFLLPIISLLIQVLNKAFRRYSSRIQDSIGEVTQVTEEVITGNRVVKV
                     FGGYEYEKDRLFEVDERNRRQNLKLVRSRALGVAVTQVIFGFGVAGVIYVAATESVEG
                     ELSPGNFIAFFGAMMLMLQPLRRLTSVSATLQRGIAAGSSLFKIIDEPDEVDSGSFRA
                     GSVQGTVEFRDVSFNYGDGKAPVLEKVSLTVEAGHSLAIVGHSGSGKSTLVSLLPRFH
                     DVDAGEILLDGTPVREYALDNLRSHVSLVSQDVVLFNDTIAHN"
     gene            complement(1337..1762)
                     /locus_tag="VFY27_00740"
     CDS             complement(1337..1762)
                     /locus_tag="VFY27_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018875790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5764065.1"
                     /translation="MKLSSQSRPEPDVNLTPLIDVVFLLLIFFMVSTSFVKQSQITIR
                     LPEAESTAMVEEPPEKIDIMITEQGTYLVNGRELINNRPETIRNALQRVSGGNTDLPL
                     TISADANARHQHVVTAMDVAGRLGFVRISIATVDDPTND"
     gene            complement(1759..2385)
                     /locus_tag="VFY27_00745"
     CDS             complement(1759..2385)
                     /locus_tag="VFY27_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5764066.1"
                     /translation="MVEIVKSGGWLMAPIILCAILTMGIILERFWTLQQKRVIPEDLT
                     SKVWGWVKKDALDQRQIQTLHQGSPLGQILAAGLINRDRERSVMKDSIEDTGRHVVHE
                     LERYLDTLGTVAAISPLLGLLGTVFGMIEVFAAITTQGNGNPTVLAGGIAQALITTAA
                     GLTVAIPALIGHRYYRNRVDTLVVNMEKEAIKLVEALHRRQARGGADS"
     gene            complement(2427..4760)
                     /locus_tag="VFY27_00750"
     CDS             complement(2427..4760)
                     /locus_tag="VFY27_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX5764067.1"
                     /translation="MLRLCLCMLAGAYALALCRELPNDAALMGTAFCTAASLCLRKLR
                     LLGFVLLGLLVMWCASRAMLEDRLLPELQGQTLAVTVKVATFPEVLPGSVRFVAQTEG
                     PTDDSMAGRVLPRKLRLSWYEPPEIPRIGETWHLQVRLKRPRGFANGTGFDYELWLAR
                     QRIGATGYVIAGPGNAKLDAVVDRRSLLRQRLVGRIVKVTGENEASAVLLAVTVGALH
                     LISKEQWERYAISGTSHLMAISGLHIGLAAGGAWLLCRLVLPLLSRSVNVRDLAALTA
                     VVAACVYAEISGLAIPARRAALMALLAAIPFILRRQLSPDKILAASCLAIFAGDPLAV
                     HAPGFKLSFGAVAILLWIARQHGGNRTGGSSRASRFLSDARSLSALQFTLLLGLFPMT
                     VLVFGRISWLAPLVNLLVLPLFNTVTVPAALLGLLLDGPFAIAGDSLLRLSWHSVRLT
                     LSLIDVVSAWPPARVHIATPTGIMPFVVMLAALPAFLPPGFPGRKLAWIAAVATLLFK
                     PAAPPGGCVDLVALEVGQGLSVVLRTRTRTLVYDTGPSFRGGSDIGQLVLVPWLRATG
                     VRQVDLLVVSHSDDDHAGGARSLVDTVKVRQLLAGEPLPSIDRPQLACRSGQAWIWDG
                     IRFGVMHPGVYPLQSNNNASCVLEVAAGRHRILLTGDIESPIENHLVRSASLSPVDIV
                     TVPHHGSRTSSGSAFVSTLRPSVAIVSAGHGNRWGFPKEDVVARWREAGARVMNTAVS
                     GAIHYRVCTEGGVQLQSEQRSRTRRYWHEGTIAGKNF"
     gene            4933..>5461
                     /locus_tag="VFY27_00755"
     CDS             4933..>5461
                     /locus_tag="VFY27_00755"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX5764068.1"
                     /translation="MPRRFFRKFALKRDRFQGQWFLAPFDHLLHDRQLWGIRRRNVVP
                     AFSLGLFIGFLPVPGHVLIAALCALALRINIPVAALTTFISNPLTVGPMYFLAYRVGL
                     KVLSLQPQPFSFELSFVWISDRFLAIWQPLLLGCILLGALAALVGYFALDLLWRASIA
                     DYIAAKREGRPGDGDT"
BASE COUNT         1066 a         1712 c         1685 g          998 t
ORIGIN      
        1 ggttgtgggc gatggtgtca ttgaacagca caacgtcctg gctgacgagg ctgacatgcg
       61 agcggaggtt gtccagcgcg tactcccgca ccggcgtgcc gtcgagcagg atctcgccgg
      121 cgtccacgtc gtggaaacgc ggcagcaggc tcaccagcgt cgatttgccg cttcccgagt
      181 ggccgacgat cgccagcgaa tggccggcct ctaccgtgag ggaaaccttc tccagtaccg
      241 gggccttccc gtctccatag ttaaagctca cgtcgcgaaa ctcgaccgta ccctgaaccg
      301 atccggcacg gaaactgccg gagtcgacct cgtccggttc gtcgatgatc ttgaacaaac
      361 tgctccccgc ggcgatgccg cgctgcagcg ttgcgctcac actggtcagg cgccgtaagg
      421 gctgcaacat cagcatcatc gcgccgaaga aggctatgaa attgcccggc gaaagctctc
      481 cttccacgga ctcggtggca gctacataga tgactcccgc tacaccgaag ccgaagatta
      541 cttgtgtgac ggcgacgccg agggcgcgcg agcgcacgag cttgaggttc tgccggcgat
      601 tgcgttcgtc cacttcgaac aggcggtctt tttcgtattc ataaccgcca aagaccttga
      661 cgacgcggtt accggtgatt acctcttccg ttacctgcgt aacctcgcca atcgaatcct
      721 gtatgcggct gctatagcgc cgaaacgcct tgttcagtac ctggatcaaa aggctgatga
      781 ttggcagcag aaacgcaacg aatatcgtca ggcgcacgct ctggtagagc attacggcta
      841 ccgccgcgat gatcgtcagc aagtcgcgga tgagaatcgt tacgacgctg gtaaccgatt
      901 ccgcgaccat ctcgacgttg taggtcatcc tcgacaacag cggccctgtc gcgtgattct
      961 cgaagaagcg cgacggcagc gtaaggaact tctggaagac gtcccgccgg agggagctga
     1021 tgacgccacg cccgatccag ccgagagaca tctcggttgc aaagccggcg aggcccctcg
     1081 caataaagat cgcgatgaac gcgaccggca tccacctgac ggtctcgagg ttgcgcgcga
     1141 ccagcgcgtc gtcggtcagg ggctgtagca gcgagaccat gccggcttcg actgctgccg
     1201 tcgcggccat gccgatcacc gcgatggcgc cgatcgcgcg atagggaaga acgtatttcc
     1261 acagtcgccc gtatacgccg gcgacctgtg gacgaatttt attcatgttg tgccctgtcc
     1321 cggaaaacag ggactctcag tcattcgtgg gatcgtcgac ggtcgcgatg ctgatgcgca
     1381 cgaagccgag ccggccggcg acgtccatag ccgtaacgac gtgttgatga cgcgcgttcg
     1441 cgtctgcact gatcgtaagt ggaagatccg tattcccgcc cgagactcgc tgcagtgcat
     1501 tgcggatcgt ttcgggacgg ttgttgatca gctcgcgccc gttgacgagg taagtgccct
     1561 gttcggtgat catgatgtcg atcttctccg gcggctcctc caccatcgcg gtgctctccg
     1621 cttccgggag gcggatggtg atttgcgact gcttgacgaa actcgtcgac accatgaaaa
     1681 aaatcagcag caggaacacc acgtcgatca acggggtcag gttgacgtcc ggctccggcc
     1741 gggactggct ggagagcttc acgaatccgc accgccccgc gcctggcgcc ggtgcagcgc
     1801 ctcgaccagc ttgatcgctt ctttttccat gttcacgacc agcgtgtcga cgcgattgcg
     1861 gtaatagcgg tggccgataa gggcgggaat cgcgaccgtg agccctgccg cagtcgtgat
     1921 gagggcctga gcaataccgc ccgccagcac cgtcgggttg ccgttgccct gcgtggtgat
     1981 ggcggcgaat acctcgatca tgccgaagac cgttcccagg aggccgagca gcggggaaat
     2041 cgcggcaacc gttccgagcg tgtcgaggta gcgctcgagc tcgtgcacga cgtgcctgcc
     2101 ggtgtcctcg atgctgtctt tcattaccga gcgttcacgg tcgcggttga tcaatccggc
     2161 agccagaatc tggcccagcg gagatccctg gtgcagtgtc tggatctgtc gctggtcgag
     2221 cgcgtccttc ttcacccagc cccacacctt gctggtgaga tcttcgggaa tgaccctctt
     2281 ctgttgcagg gtccagaaac gttccaggat gatgcccatc gtgaggatgg cgcacaggat
     2341 gatcggcgcc ataagccagc cgccggattt aacgatttca accatatagc gtgacccggt
     2401 gaaaagcgga atatacagcg cccggctcaa aaattcttcc cggctatcgt accttcgtgc
     2461 cagtaacgcc gcgttcggct gcgctgctcg ctctgcagtt gcacgccgcc ttccgtgcag
     2521 acgcgataat gaattgcccc ggacaccgcc gtgttcatca ctcgggcgcc cgcttcccgc
     2581 cagcgcgcca ctacgtcctc tttcggaaag ccccagcgat tgccgtgtcc ggccgacacg
     2641 atggcgacgg acggacgcaa cgtgctgacg aatgcgctgc cggaggaagt ccggctgccg
     2701 tgatgcggaa cggtgacgat gtcgacaggg gacaacgatg ctgatcgcac aaggtgattt
     2761 tcgatcggcg attcgatatc gccggtaagc aatatcctgt gccggccggc ggcaacttcg
     2821 agcacacagg atgcgttgtt gttgctctgc agcggataga cgcccgggtg cattacgcca
     2881 aagcggattc cgtcccaaat ccaggcctgt ccgctgcggc acgcaagctg cggccggtcg
     2941 atcgatggaa ggggctcacc tgccagcagc tgcctcacct tgacggtgtc taccagggaa
     3001 cgggcgccgc ccgcatggtc gtcatcggaa tggctgacga cgagcaggtc gacctgcctc
     3061 acgcccgttg ctctcagcca tggcaccagt accagctgcc cgatatcgct gccgccgcga
     3121 aacgacgggc cggtgtcgta tacaagggtg cgggttcgcg tccgcagcac caccgacagt
     3181 ccctgaccga cttccagcgc gacgaggtcg acgcagccac cagggggcgc cgcgggtttg
     3241 aaaagaagcg tcgctaccgc ggcgatccag gcaagcttgc gaccgggaaa gcccggaggg
     3301 aggaaggccg gcagcgcggc aagcatcact acgaacggca tgattccggt cggcgtggcg
     3361 atgtgaaccc gcgccggcgg ccacgcggac acaacgtcga tcagggaaag tgtcagccgc
     3421 acgctatgcc aggacaggcg aaggagacta tcgccggcaa tcgcgaaagg accgtcgagc
     3481 aacagcccca gcaatgccgc cggcacggtg acagtgttga atagcggcag caccagcagg
     3541 tttacaagcg gtgcaagcca cgagatgcgg ccgaagacga gcaccgtcat cgggaacagt
     3601 cccagaagga gtgtgaactg cagcgccgac aggctcctgg cgtccgacag gaagcggctt
     3661 gcccgggacg atccgccggt cctgttgccg ccatgttgcc tcgcgatcca caggaggatt
     3721 gctaccgcgc cgaacgatag tttgaaaccc ggtgcgtgaa cagccagcgg atcgccggcg
     3781 aagatcgcga gacaactcgc ggccaggatc ttgtccggtg acagctggcg ccgcaggata
     3841 aacgggattg ccgccagcaa cgccataagt gccgcgcgcc gcgcgggaat agccaggccg
     3901 gagatttcgg catagacgca agccgccacg accgccgtca atgccgccag atcgcggacg
     3961 tttactgaac gggaaagcag cgggagtacc agccggcaca gcagccaggc accaccggcc
     4021 gcgaggccga tgtgcagccc ggatatcgcc atcaggtgac tcgtgccgct gatggcgtaa
     4081 cgctcccact gctccttgct gatgaggtgg agtgcaccga cggttacggc gagcagcacc
     4141 gccgacgcct cgttctcccc ggtaaccttc acgatccggc caacgagacg ctgtcgcagc
     4201 aaggagcgcc ggtccacgac ggcgtcgagc ttcgcattcc cggggccggc gatcacatag
     4261 ccggttgcgc cgatgcgctg gcgcgccagc cagagctcat aatcgaatcc cgtgccgttg
     4321 gcaaacccgc ggggacgttt caggcgtacc tgcaggtgcc atgtctcgcc aatgcgcgga
     4381 atttccggcg gttcgtacca gctcagccgg agcttccggg gaagcacgcg cccggccatc
     4441 gaatcatcgg tgggaccttc ggtctgcgcg acgaagcgaa cggagcccgg cagaacctcg
     4501 gggaaggttg cgactttcac cgtcacggcc agcgtctgcc cctgcaactc cgggagcagc
     4561 cggtcttcca gcatcgcgcg cgaggcacac cacatcacga gcaggccgag caacacgaag
     4621 ccaagcaggc gcagcttccg caggcagagg cttgcggctg tgcagaatgc cgtgcccatg
     4681 agggcagcgt cgttcggaag ctcgcggcaa agcgcgagcg cataagcacc ggccagcatg
     4741 cacaggcaga gtcgaagcat tttccgcttc cctgcggttt gtcgttatca tgctcgccct
     4801 aacccggact attcatgaat gcactcgtgc cgtctgcttt gccatcgtca cgtcggtcca
     4861 tgaataatcc ggctgcgatg ccgtagacgt cggcatcgaa actcaagcca taagaccaaa
     4921 ctccagctgc cgatgccaag gcgatttttt cgcaaatttg cactgaaacg cgaccgattc
     4981 cagggccaat ggtttcttgc acccttcgat cacctgctgc acgacaggca actgtggggg
     5041 atcaggaggc ggaacgtggt gcccgcgttt tcgctgggcc tcttcatcgg gttccttcca
     5101 gtaccagggc acgtcctgat cgcagcgctc tgtgcgcttg cgctgcgaat caatatccct
     5161 gtcgctgcgc tcaccacttt tatcagcaat ccgctgacag tggggccgat gtattttctc
     5221 gcgtaccggg tggggctgaa agtcctgagc ctgcagcctc agcctttctc cttcgagttg
     5281 tccttcgtct ggatcagcga ccggttcctc gcgatctggc agccgttgtt gctcggttgc
     5341 atccttctgg gtgcactggc ggcattggtg ggctacttcg cgctcgacct gctgtggcgc
     5401 gcatcgatcg ctgactatat tgcggcaaag cgcgaaggaa ggccgggcga cggggacacg
     5461 g
//
LOCUS       DASUOM010000035         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1794009,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000035 DASUOM010000000
VERSION     DASUOM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794009"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..>2918
                     /locus_tag="VFY27_00760"
     CDS             392..>2918
                     /locus_tag="VFY27_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX5764069.1"
                     /translation="MLAEAEASQLLGAWKGVTTAYPREATIGGLFLETARRFPQRVAL
                     CSGGRSLTYFQLRSEASQLALHLLEAGVAPGDLIAICSRPSPEMIVGFLAILLAGGCC
                     VPIDPTYPADRFTQLLEDCGATIGLAMVGCEACYPADWKGKILLIHAAGDAAAPTGLP
                     EIPLTAEHPAHLLFTSGSTGRPKGVLLAHRGVVRLVRNQDFITITPDDVFLQAAPASF
                     DASLLEIWGPLLNGGRLILLPDGPGIAEIVTAVREHGVTTLWLTSGLFQLMMDASPAS
                     LKGLRYLLSGGDVLSPPHVRRALESLPGTRLINGYGPTENTTFTTCHAITLADLEKSS
                     IPIGKPIANTTVYLLDGMLRLVPVGVPGELFAGGDGLAIGYHGAAELTAEKFITHPEF
                     GRLYRTGDLCRRTTDGTIEFLGRVDHQVKVRGFRIELGEIESVLGSHPDVCQAKAAVR
                     GESAEAKRILAWVIPNELGKVQEIDLTRFLAARLPAYMRPDAVGIVESFPLNANGKIQ
                     VSALPDPARSWSATLARAAAPPAGETEKQLAVIWSELLDMADINRDDDFFALGGHSLM
                     ALRMFARIHHEFDRSLPLATLLEHPTIEGLAGVLDPSVEVKAGEAPPIPGKGNVVTIA
                     VGGDDTPLCCIHGGDGGVLFYRSLAALLPPEIPLHAIESLELGSSGPIESSSIEETAA
                     GYICSLRAVQPHGPYRLAGYSFGGVVAHEMAFQLTRDGHAVEFVGLFDTHNPAAPARF
                     YTLTERLRVFWRQNSAVPVGRRLKLVHARLSEGIRANRRVKAETRAAQRSGPAEPYSD
                     LRRVQVRQENWHAMQAYRPPAYQGRITLFKTSMISDKVERPADY"
BASE COUNT          533 a          954 c          894 g          537 t
ORIGIN      
        1 catacctggg cgcttggctg agcgggcccg cctagcgccg ataaatctgg cggcactgcc
       61 agcaccggct tcgaacgtgg ttttcgaagt cgattcaggt gacatttggt gagcaaaaga
      121 aacggcctac tcgtgggctg cggggagggc tgcatccaca gcacgccatt gccaactccc
      181 cagaacgtcg ccgcccccag gcgacaacaa ctgcttcaaa ccgtgcagat cagatgcgtt
      241 tgaggcccca gacctggcga cactcccgca atgatgtggt tgcaatttcg gttccccgtt
      301 gttgcgcccg tggaagaatt cgcgacaacc tccatccagt ctgtcaaccg tgatgcctat
      361 cgccgaattc tccgagccga taagccaagc aatgcttgct gaagcggagg catcccaact
      421 cctcggagca tggaaaggcg tcaccaccgc ctacccgcga gaggcgacca tcggcgggct
      481 tttcctggag accgcgcgcc gcttcccgca gcgcgtggcg ctgtgttccg gtggccgctc
      541 gctcacgtat ttccagcttc gttcggaggc gagccagctc gcgctgcact tgctcgaagc
      601 cggcgtcgct cccggagatc tgatcgcgat ctgctcgcgt ccttcgccgg aaatgatcgt
      661 cggattcctc gccatcctgc tcgctggcgg ctgttgcgtg ccgatcgacc ccacctatcc
      721 ggcggaccgc tttacgcaac tgctggagga ttgtggcgcg accatcggcc tcgcgatggt
      781 gggatgcgag gcctgctatc cggcggattg gaagggaaaa atcctcctga ttcatgcggc
      841 cggagacgcc gcagccccca ccgggcttcc ggagattccg ctgacggcgg agcatccggc
      901 gcatctgctt ttcacctcag gctccaccgg acggccgaaa ggcgtcctgc ttgcccatcg
      961 cggcgtggtg aggttggtca ggaatcagga tttcatcacg atcacgcctg acgacgtctt
     1021 tctgcaagcc gcgccagcgt cgttcgacgc atcgcttctc gaaatctggg ggccgctgct
     1081 caacggcggc cgcctgatcc tgctccccga tggtccgggg atcgcggaaa tcgtcacagc
     1141 ggtccgcgaa cacggcgtca ccacactttg gctcacgtcc gggttgtttc aactgatgat
     1201 ggatgcaagc ccggcatctc tcaaagggct gcgctatctt ctgtcgggag gcgacgttct
     1261 gtctccgccg catgtgcggc gggcgctgga atccctgcca ggcacccggt tgatcaacgg
     1321 ctatggaccc accgaaaaca cgacattcac cacctgccac gccatcaccc tggcggatct
     1381 ggagaagtcg tcgatcccga tcggcaagcc gatcgccaac accacggtct atctgttgga
     1441 tggcatgctc agactggtcc cggtcggcgt tcctggcgag cttttcgcag gcggtgacgg
     1501 cctcgccatc ggctaccacg gcgcggccga gcttacagcg gagaaattca tcacccatcc
     1561 cgagttcggc aggctctatc gcaccggcga tctctgccga cggacgaccg acggcaccat
     1621 cgagtttctg ggccgtgttg accatcaggt aaaggtccgc ggcttccgga tcgaactggg
     1681 ggaaatcgaa tccgttctcg gcagccaccc ggacgtctgc caggccaagg cagccgtacg
     1741 cggagagagc gccgaagcca aacgcatcct cgcctgggtg attcccaacg agctcggcaa
     1801 ggttcaggag atcgatctga cgagatttct cgcggcccgg ttgcccgcct acatgcgacc
     1861 cgacgccgtc ggcattgtcg aatcgttccc gctcaacgcc aacggcaaga ttcaggtgtc
     1921 tgccctgcca gaccccgcga gaagctggag cgcgacgctg gcgagggctg cggcaccgcc
     1981 tgctggagag acggaaaagc aactggcggt gatctggagc gagctgctcg acatggcgga
     2041 catcaaccgt gacgacgact ttttcgccct cggcggccac tcgctgatgg ccctgcggat
     2101 gtttgcccgg atccaccatg agttcgacag atcactgcct ctggccacac tgctcgaaca
     2161 tcctacgatc gaaggtcttg ccggcgttct ggacccatcg gtcgaggtga aagcgggcga
     2221 agcgccgccg attccgggca aaggcaatgt cgtcacgatc gcggtcggag gcgacgacac
     2281 gccgctgtgt tgcattcacg gcggcgatgg cggggtgctg ttctaccgca gtctcgccgc
     2341 gttgttgccg ccggaaattc cgctgcatgc catcgagtcg ctggagctcg gcagcagcgg
     2401 ccccatcgaa tcctccagca tcgaagaaac ggcggcaggc tatatctgca gcctgcgggc
     2461 cgtgcagccg cacgggcctt accgccttgc cggctactca ttcggcggcg tggtggcgca
     2521 tgaaatggcc tttcaattga cccgggacgg acacgcagtt gaattcgtcg gcctcttcga
     2581 cacccacaac ccggccgcgc cggcacggtt ctacacgctg acggagcgcc tgcgtgtttt
     2641 ttggcgacag aactcggcgg tgccggtagg acgccggctg aagctggtgc atgcgcgatt
     2701 gagcgaaggg atccgggcca accgccgggt gaaggcggaa accagggccg cacaacgcag
     2761 cggtccggcg gagccctaca gcgatctgcg ccgcgtgcag gtgcgccagg aaaattggca
     2821 tgccatgcag gcctaccgac cccctgctta ccagggacgc atcacccttt tcaagacctc
     2881 aatgatcagc gacaaggtcg agcgtccggc cgactatg
//
LOCUS       DASUOM010000036         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1308828,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000036 DASUOM010000000
VERSION     DASUOM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308828"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VFY27_00765"
     CDS             <1..2145
                     /locus_tag="VFY27_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764070.1"
                     /translation="PQGRFVLGPNFGFWDGTLNDGFVNDGTGPLPAFDPLDPDGGDFN
                     AFDAGDRFNFNGPGFNYLRTPNERINVYSNVTHQLSDTVEMFIRGSYTNRASATKAAP
                     EPLCLGNGCGNRINDNFVISALNPFNPFGVDLSVANGNLEFFGRRPLESGPRLFFQEV
                     DALMLSAGLMGEFAAGSRTMYWDLSAGYGDNRGFQEKENSHNAAKLQVAMGDPAVCAA
                     TPNCVPFNFFGGQGANGEGSITEEMLDFVGYTQRDYSTQTLRDFAFNITGDLMQLPGG
                     DMGFAAGIEYRDHSGSYRPDPIAERGETAGIPAGATRGSFDVTEYYAELSLPLIAGAP
                     LADYVEVNIAARNSDYSTSGSEATYKVSGLWRPIEELSFRASFSTGLRAPGIGELFGG
                     AAREDFTFLDPCADYTGIVGSANGGRDAPQPANIQANCAALGVPVGLAQLNPQQSVVN
                     AGNSALDPETSDNWTAGLVWSPSWADDSEWASGLTMSLDFYNLEIDDAIQGVPPGDLV
                     TACVDTLSPVFCDSVPRTSSGQLGLVNNQLQNIGGIEASGFDLMLNYLTPELGFGQFG
                     LTVNATHLDEYLEKTAGLTGGSETVTDRTGEHTQETFQRAFPEWRAVTTIDWLMDRWS
                     GALSFRWVDEMFIVVNPTTRNQLDSAMFTDLRFSYNPAFADDALTLTLGFNNVLNEDP
                     PVCDPCGSIGMSRVAHDIPGTVGYLRVTYQHE"
     gene            2240..2887
                     /locus_tag="VFY27_00770"
     CDS             2240..2887
                     /locus_tag="VFY27_00770"
                     /inference="COORDINATES: protein motif:HMM:NF039641.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HEX5764071.1"
                     /translation="MRLPFTSSEFFQVFADYNETVWPAQILLLLIALVATALMFSRAR
                     WSDTLISTLLAALWLWMGLVYHLSFFARINPLAYAFAALSIAGAAAFLWHGLYRKRLQ
                     FRWRSSGWNVAGAFLIIFSLAVYPVWTWRTGHTWPAMPTFGLPCPTTLFTIGMLAFLL
                     PPYPRSVLLAPLLWCAIGVQAAFLLDVTPDLSLLAGGTVAVALLVVAGRSRETDL"
     gene            complement(2999..4468)
                     /locus_tag="VFY27_00775"
     CDS             complement(2999..4468)
                     /locus_tag="VFY27_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5764072.1"
                     /translation="MAIITRRALLRGTAAISGLAAIPAPAVFGLESLSRASNASPLDG
                     LAKMLRGELVIPGHENYEAMRRVWNGMIDRRPAAIARCTGAADVMAVVKYARDNGIAA
                     TVRGGGHHVAGKSVRDGAIVIDLAPMQGSQVDPVKRRARAEGGLRWGALDHETLAHGL
                     YTTGGTVSVTGIGGLTLGGGLGWLMRKHGLSCDNLVGAEVVTADGRLLSANASENSDL
                     FWALRGGGGSFGVVTAFDYRLHEVEPIVGGLAVYAGERLKDMLHFYRDFTANAPDHLE
                     TQAGAMIGPPGTPVEGQVAGWMAICHSGPEADGERIVAPLKDFGPPVMDFIGPTTYST
                     IQTMFDAGMAITTRNYWRSSFMKELGDDVLDSLVDHAADMPRPGTLLLIEHMGGAIRR
                     VGEQETAFANRGAEYNVSVLSSWLDPEADAKNIAWTRQFGDDLNSFSSGGAYVNYMAN
                     SDDPAAMRAAYEANFERLAEVKRKYDPDDFFNPGEILKR"
BASE COUNT          814 a         1450 c         1406 g          922 t
ORIGIN      
        1 ccgcagggcc ggttcgtgct cggtccgaac ttcggtttct gggatggcac attgaatgac
       61 gggttcgtga acgacggcac gggcccgctc ccggccttcg acccgctcga tccggacggc
      121 ggcgatttca atgccttcga cgcaggcgac aggttcaact tcaacggacc ggggttcaac
      181 tatttgcgca cgccgaacga gcgcatcaac gtctacagca atgtgacaca ccagttgtcc
      241 gacacagtgg aaatgttcat tcgcgggtcc tacacgaacc gtgcctcggc aaccaaggcc
      301 gcgccggaac ctctgtgtct tggcaatggt tgcggtaacc ggatcaatga caacttcgtg
      361 atttcggctt tgaatccgtt caaccctttc ggcgtggatc tttcggtcgc caacggcaac
      421 ctggaatttt tcggacgccg tcccctggag tccggaccgc gcctgttctt ccaggaagtc
      481 gatgccctga tgctgagtgc aggtctgatg ggagaattcg ccgccggcag ccgcacgatg
      541 tattgggacc tgagcgccgg ttacggcgac aaccgtggct tccaggaaaa agaaaattcg
      601 cataatgctg ccaagctgca ggttgcgatg ggagacccgg ctgtttgtgc cgcaaccccg
      661 aactgcgtgc cgttcaattt cttcggcggc caaggtgcga acggagaagg ctcgatcacg
      721 gaggaaatgc tcgacttcgt gggatatacc cagcgcgatt acagtaccca gacgctccgg
      781 gacttcgctt tcaacatcac cggcgatctc atgcaactgc ctggcggtga catgggcttc
      841 gccgcaggca ttgagtatcg cgatcacagc ggttcgtacc ggcccgaccc gattgccgaa
      901 cgtggcgaaa cggcaggtat cccggccggc gcgaccaggg gttcgttcga cgttaccgag
      961 tactacgccg agctcagcct gccgctgatt gcgggggccc cgcttgccga ctatgtggaa
     1021 gtgaacattg ccgcacgcaa ttccgactac agcacttcgg gctcggaagc gacttacaag
     1081 gtcagcggcc tgtggcggcc gatcgaggag ctgtcgtttc gcgcgtcgtt ctctactggc
     1141 ctccgcgcgc cgggtatcgg tgaactgttc ggcggcgctg cgcgcgagga tttcacgttc
     1201 ctggatccct gcgccgacta taccggcatc gtaggttccg ccaacggcgg gcgtgacgcg
     1261 ccccagccgg caaatatcca ggccaactgc gccgcgttgg gcgtacccgt gggcctggca
     1321 cagctgaatc cacagcaatc ggtggttaat gcgggcaatt cggcgctcga tccggaaacc
     1381 tccgacaact ggacggccgg cctggtctgg agcccgagct gggccgacga ctcagaatgg
     1441 gcctccggcc tgaccatgtc gctggacttc tacaacctgg agatcgacga cgcaatccag
     1501 ggcgtgccgc cgggcgacct cgtaacggcc tgcgtagaca cgctgagtcc ggtgttttgc
     1561 gactccgttc cgcgcacgag ttcaggtcag ctcgggttgg tcaacaacca gctgcagaac
     1621 atcggtggca tcgaggcttc cggcttcgac ctgatgctga actacctgac cccggaactc
     1681 ggcttcggcc agttcgggct gacggtgaat gcgacgcatc tggacgagta cctggaaaag
     1741 acagccggtc tgaccggtgg ttcggagacc gtcacggatc gcacagggga gcatacgcaa
     1801 gagaccttcc agcgcgcgtt tcccgagtgg cgtgccgtga ccacgatcga ctggctcatg
     1861 gaccgctgga gcggggccct gtcattccgc tgggtcgacg aaatgttcat cgtcgtgaat
     1921 cccaccacgc ggaaccagct ggattcggcg atgtttaccg acctgcgttt cagctataac
     1981 ccggcgtttg ccgacgatgc gctgactctg acgctcggct tcaacaacgt gctgaacgaa
     2041 gatccgccgg tgtgcgatcc ctgcggcagc attggaatga gccgcgtggc acacgacatc
     2101 ccgggaacgg tcggctacct tcgtgtgacc taccagcacg agtgaaagcg ccgcgcagtt
     2161 ctgcgcaata caagacggcc ccttcggggg ccgttttttg tggtcagatg gccctggagt
     2221 aacgtcggac cgcaaccgta tgcgcctgcc attcacatca tccgagttct tccaggtttt
     2281 cgccgactat aacgagaccg tctggcctgc ccagatcctg ttgctgctga tcgcgctcgt
     2341 cgccacggca cttatgttca gccgggcgcg ctggtcggat acattgatat cgacgcttct
     2401 cgcagctcta tggttgtgga tgggtctcgt ctatcacctg tccttcttcg cccgtatcaa
     2461 tcctctggca tatgcgttcg ctgcgttgtc gatcgccggt gcggctgcct ttctctggca
     2521 tggcctgtac cgaaagcgcc tgcagttcag atggcggagc agcggttgga atgtggcagg
     2581 tgcattcctg atcattttct ccctggccgt ttaccctgtt tggacctggc ggaccggaca
     2641 tacctggccg gcgatgccga cattcggctt gccgtgccca acgaccctgt ttacgatcgg
     2701 gatgctggcc ttcctgctac cgccgtaccc acgcagcgtg ctgctggcgc cgctcctctg
     2761 gtgcgcgata ggcgtgcaag ctgcgttcct gttggatgtc acgccggacc tgtcgttgct
     2821 cgccggcggc acggtggccg tcgccctgct ggtggtggcg gggcgatctc gtgaaacgga
     2881 tttatgatcg agttcgtgct gtcgtgtacc gctgtgccac gaagtcaatc ggaggcgcat
     2941 tcgcgacacg caggtcgacg agccatgtga ggcttcgctt ttggggaccg cccgtccttc
     3001 accgcttcag gatttcgccc ggattgaaga agtcgtccgg atcgtacttc cgcttgacct
     3061 cggcgagtcg ctcgaagttg gcctcgtagg ctgccctcat cgccgccgga tcgtcgctgt
     3121 tcgccatgta gtttacgtac gcgccgccgc ttgaaaacga attcaggtcg tcgccgaatt
     3181 gtcgagtcca ggcgatgttc ttcgcgtccg cttccggatc gagccagctc gacagcacgg
     3241 acacgttgta ctcggcgccg cggttggcga atgccgtttc ttgctcaccc acccggcgga
     3301 tcgcgccacc catatgctcg atcaacagga gcgtgcccgg ccgcggcatg tcggcagcat
     3361 gatcgacgag ggagtccagc acgtcgtcgc caagttcctt catgaaactc gagcgccagt
     3421 aattgcgggt cgtgatcgcc atgcccgcat caaacatcgt ctggatggtc gaatacgtgg
     3481 tcgggccgat gaagtccatc accggcggcc cgaaatcctt gaggggtgcg acgatacgtt
     3541 cgccatcggc ctccggaccg ctgtggcaga tcgccatcca accggcgact tgcccttcca
     3601 ccggagtacc gggtggaccg atcattgcgc ccgcctgggt ttcgaggtga tccggcgcgt
     3661 tcgcagtgaa gtcgcggtag aaatgcagca tgtctttcag gcgttcgccg gcatagacgg
     3721 ccaggccgcc aacaatcggc tcaacctcgt gcagtcggta atcgaatgcg gtgacgacgc
     3781 cgaagcttcc gccgccaccg cgcagcgccc agaacaggtc gctgttctcc gaggcattcg
     3841 ctgacagaag acggccgtca gccgtcacga cctcggcgcc gaccaggttg tcgcagctca
     3901 aaccgtgctt gcgcatgagc cagcccaggc ccccgccaag cgtcagcccg ccgataccgg
     3961 taaccgagac ggtgcctccg gtcgtgtaca ggccatgcgc caacgtttca tgatcgagtg
     4021 cgccccagcg gagaccgcct tccgcgcgag cgcgcctctt tacgggatcg acttgcgatc
     4081 cctgcatcgg ggcgagatcg atcacaattg ctccgtcacg aacggacttt cccgccacat
     4141 gatggccgcc gccacgcact gtcgcggcga tgccgttgtc gcgagcgtac ttgacgacag
     4201 ccatgacgtc ggcggcgccg gtacagcggg cgatcgccgc gggccgccgg tcgatcatgc
     4261 cgttccagac gcggcgcatg gcctcgtagt tttcgtgccc ggggatgacc aactccccgc
     4321 gcagcatctt ggcgagcccg tcgagtggcg aagcgttcga cgccctggag agggactcca
     4381 gcccgaagac agccggtgcc ggaatcgccg cgagtccgct tatcgctgcc gtgccacgta
     4441 acagcgcgcg ccgagtgatg atagccatct cgcatctcca tgagggctta cggggccaaa
     4501 aatactaaca gcagttgccg cctgatgcat tcaggcaccc gagggctcgt gcaggggggg
     4561 atcagtatca gccttcagtg ggcacgcaat ct
//
LOCUS       DASUOM010000037         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_829602,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000037 DASUOM010000000
VERSION     DASUOM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829602"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFY27_00780"
     CDS             <1..541
                     /locus_tag="VFY27_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX5764073.1"
                     /translation="DMSHNIACCLVGHRRFTLFPPEQVHNLYPGPLDPTPGGQVVTMT
                     DIQAPDFDRFPRLREALAVSEVADLEPGDALFYPAMWWHQVDALDSFNVMINYWWNTL
                     PAYIDTPQNALLHALLSIRDRPEQEKRAWRAMFDYYVFGPPERAGEHLPSGARGNLGP
                     LDETKARRLRAYLLKRLNR"
     gene            550..2040
                     /locus_tag="VFY27_00785"
     CDS             550..2040
                     /locus_tag="VFY27_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX5764074.1"
                     /translation="MSRQVVMRVVIAGGGTAGWTAAAALAQQLGPLLEITLVESDEIG
                     TVGVGEATIPTFRTFQVLLGLDEREVMRATQATFKLGIKFENWSQLGHRYFHAFGDIG
                     KSTWMGDFHNMWLMAKARGFGGELGDYCFEHQAAEAGRFATSDKAQINYAYHLDAGLY
                     AHFLRKKFEPKGVRRIEGKIERVEQDGESGYVTALVLQNGTRIEGDLFVDCTGFRGLL
                     IEQTLGAGFDDWRHWLPTDSALAVQTESTGRLPPYTRSIARTAGWQWRIPLRHRVGNG
                     LVFCSTHQAEDEARAELLGNLESRPLFEPRLIRYVTGRRRKAWHKNVVALGLASGFLE
                     PLESTSIHLIQIGVMRLMQLFPFEGNFESLAVRYNEQSHREFESIRDFLVLHYKLTER
                     EDAPFWRDCGAMAIPDTLAARIELFLDSGYAFRGPHDLFVVASWLFVMVGQGLMPRHH
                     HYMGALLGDERLRRALESLSSKIERAVARLPRHEDFLNHYCPAADV"
     gene            2091..>3557
                     /locus_tag="VFY27_00790"
     CDS             2091..>3557
                     /locus_tag="VFY27_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX5764075.1"
                     /translation="MNFTVRIFSVAVLCCVAAAALAEAADELQINDKDYFEAPGLNVL
                     VFSNWYDGLFSDSKISGIELIHHGVRTATNGDVRLNATPEQWDAIPTFVERKVDAATN
                     SIEAFLRYPAFDFDFSIKAEGGQDGVLISVHLPEPLPAELEGKAGFNLEFLPAAYFEK
                     AFMMDGESGLFPLYPGGPKEQNDRLAPEPLAVGSRLVLAPDDPERTVSISSPDALLSL
                     YDGRAKAQNGWFVVRSLLPAGRSGEVLQWTLKPKAKENWTRDPVILHSQVGYHPQQEK
                     LAVIELDRNDRTVDAVRLVRVTPKGEAKQVAQEQPAEWGQYLRYRYKIFDFSTVTDEG
                     IYYLEYGSQRSTPFRIADDVYRDVWKPTLDVFLPVQMDHVTVNEAYRVWHGASHLDDA
                     LQAPTDHEHFDLYAQGPTTDTPYRPGEHIPGLNVGGWYDAGDYDIRTQTQYYVVNNLV
                     DAWEEFGITRDNTSVDYERKFVDIHVPDGKPDLLQQIEH"
BASE COUNT          696 a         1073 c         1133 g          655 t
ORIGIN      
        1 cgacatgtcg cacaacatcg catgctgcct cgtgggtcac cggcgtttta cattgtttcc
       61 cccggagcag gtgcataacc tgtatcccgg cccgctggat ccgaccccgg gtggacaggt
      121 ggtcacgatg acggacatac aagcacccga tttcgaccgg tttccgcgtt tgcgcgaggc
      181 gcttgccgtg agtgaagttg cggatctcga gccgggcgac gcactgttct acccggcgat
      241 gtggtggcac caggtggatg ccttggactc gttcaacgtg atgatcaatt actggtggaa
      301 tacattgccg gcttatatcg acacgccgca gaacgcgctc ttgcacgcgc ttctgagcat
      361 ccgtgaccgg ccggaacaag agaagcgggc gtggcgcgcg atgttcgact actacgtgtt
      421 cggcccgccg gaacgggcgg gcgagcacct gccgtccggc gcgcgtggca accttgggcc
      481 gctggacgag accaaggccc ggcgcctgcg tgcttacctg ctgaagcggc tgaaccggtg
      541 acggcgccca tgtcccggca ggttgtaatg cgcgtcgtca tcgccggagg cggcacggcc
      601 ggctggaccg cggccgcggc gctcgcacag cagctcggtc ccctgctcga aatcaccctc
      661 gtcgagtccg acgagatcgg gaccgttggc gtcggcgagg cgacgatccc gacgttccgt
      721 accttccagg tgctgcttgg actcgacgaa cgcgaggtta tgcgcgcaac gcaagcgacc
      781 ttcaagctgg gaatcaagtt tgagaactgg agccaattgg gtcatcgcta ctttcacgca
      841 ttcggcgaca tcggcaagtc gacctggatg ggcgacttcc acaacatgtg gctcatggcg
      901 aaggccaggg gtttcggcgg tgagctcggg gactattgct tcgagcatca ggcggccgag
      961 gccggaaggt tcgcgacctc ggacaaggca cagattaatt atgcgtatca tctcgacgcc
     1021 gggttgtacg cgcactttct acggaagaag ttcgagccga agggcgttcg gcgcatcgag
     1081 ggcaagatcg aacgggtcga gcaggacgga gaaagcggct acgttacggc gctcgtcctg
     1141 cagaacggta cgcgcatcga gggagacctg tttgtcgact gtaccggttt ccgcggcttg
     1201 ctgatcgagc agacactcgg cgctggcttc gacgactggc ggcactggct gccaactgac
     1261 agcgcgctcg cggtgcagac agaatcgaca ggccggctgc caccatacac ccggtcgatt
     1321 gcgcgtacgg ccggctggca gtggcgcatt ccgctgcggc atcgggtggg caacgggctt
     1381 gtgttctgca gtacgcacca ggcggaagac gaagcccgtg ccgagttgct cggaaacctc
     1441 gagagcaggc ccttgttcga gccgcggctc atccgctacg tcaccgggcg ccgtcgcaag
     1501 gcatggcaca agaacgttgt ggcgctgggg ctcgcaagcg gcttcctcga accgctggaa
     1561 agcaccagca tccacctcat ccagatcggc gtcatgcgac tgatgcagct cttccccttc
     1621 gaggggaatt tcgaatcact tgcagtccgt tacaacgagc agtcgcaccg ggaattcgaa
     1681 agcatccgcg actttcttgt gctgcactac aagctcaccg agcgcgagga tgcgccgttc
     1741 tggcgggact gcggcgccat ggcgataccc gatacactcg ccgcgcgaat cgagctgttc
     1801 ctggatagcg gatacgcatt tcgcgggccg cacgatttgt tcgtagtcgc gtcatggctg
     1861 ttcgtgatgg taggacaggg ccttatgccg cgccatcatc attacatggg tgcactgttg
     1921 ggcgacgagc ggctgcgacg ggcactcgag tcgctgagca gcaagatcga gcgtgccgtg
     1981 gcgagactgc cgaggcacga agatttcctc aaccactatt gtccggcggc ggacgtgtag
     2041 ttggtggctc gcgtgaaacc ttaccgggcg ccattgccag gacgggcatg atgaacttca
     2101 ctgtgcgaat cttctccgtc gcggtgctct gctgcgtcgc cgctgcggcg ctggccgaag
     2161 cagccgacga attgcagatc aacgataagg attatttcga agcgcccggc ctgaatgtgc
     2221 tggtgttcag caactggtac gacgggttgt tcagcgactc caagatcagc ggcatagagc
     2281 tcatacatca cggtgtccgg acagcgacca atggcgacgt ccggctgaac gcgacgcccg
     2341 aacaatggga tgctatcccg acgttcgtgg aacgtaaggt cgacgcggcg accaattcca
     2401 tcgaggcatt cctccggtat ccggcattcg atttcgactt ctcgataaag gcggagggcg
     2461 ggcaagacgg cgtgctgata tccgtgcacc tgccggaacc cctgcctgcg gaactcgaag
     2521 gcaaggcggg tttcaacctg gagttcctgc cggctgccta cttcgagaag gcgttcatga
     2581 tggatggcga gtcgggtctg ttcccgcttt atcccggcgg accgaaggaa caaaacgacc
     2641 ggctggctcc ggagccgctg gcggttggct cgaggctcgt cctggcgccc gatgacccgg
     2701 agcgaacggt ctcgatctcg tcccccgatg cgctgctctc gctgtacgac ggcagggcga
     2761 aagcacagaa cggctggttc gtcgtccgca gccttctgcc tgccgggagg agcggcgagg
     2821 ttctgcagtg gactctcaaa ccgaaagcga aggaaaactg gacaagggac ccggtgatcc
     2881 tgcattcgca ggtgggttat cacccgcaac aggaaaagct tgccgtcatc gagctggaca
     2941 ggaacgaccg tactgtcgac gccgtgcggc tcgtgcgtgt cacgccgaag ggggaagcga
     3001 aacaggtcgc gcaggagcag ccggctgaat ggggccaata cctgcgctac cggtacaaaa
     3061 ttttcgattt ctcgacagtc accgacgaag gcatctacta cctcgagtac ggatcgcagc
     3121 gttccacgcc gttccgcatc gccgacgacg tgtaccgcga cgtctggaaa ccgacgctgg
     3181 atgtcttcct gccggtgcag atggaccacg tcactgtcaa tgaagcatac cgcgtgtggc
     3241 atggtgcctc gcacctggac gatgcgctgc aggcgccgac agatcacgaa cacttcgacc
     3301 tgtatgccca agggccgacg accgatacgc cgtaccggcc gggggagcac attcccggac
     3361 tgaacgtcgg aggctggtat gacgccggcg actacgacat ccgcacgcag acccagtact
     3421 acgtcgtcaa caatctcgtg gacgcgtggg aagagttcgg cattacccgc gacaacacca
     3481 gcgtcgacta cgaacgcaag ttcgtcgaca tccacgtccc tgacggcaag cccgatctcc
     3541 tgcagcagat cgagcac
//
LOCUS       DASUOM010000038         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1677347,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000038 DASUOM010000000
VERSION     DASUOM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677347"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFY27_00795"
     CDS             <1..388
                     /locus_tag="VFY27_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764076.1"
                     /translation="QVTSERTASLAVIHLGSDDVLTQIYPNPLGFTPQLEGNAANGVG
                     GEIGLAAREPIGVEWFVFIASESLPLPPTMPGLQQVGSWATVYPLGEQGSPGEKLALW
                     LADTFGGAGVSSNILQIEVVAGEPAP"
     gene            385..1428
                     /locus_tag="VFY27_00800"
     CDS             385..1428
                     /locus_tag="VFY27_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5764077.1"
                     /translation="MSAIPQEIFNSALEYFLDPIKELLADESVREIMINGVGQIYIEK
                     KGSLEKSSITFPDEDQLLAAVRNIAQYVGHYIRPGTARFDARLPQGHRVHVVLPPVCR
                     QGISVTIRKHTKSAFGLEDLVRLGSINEESKDFLRLCMEREKNMVISGGTGTGKTTFI
                     NALSALIPKGERIITIEDAAELQLDQEHVVSLEARPPDRKGEGAVTIRDLLHSSLRMR
                     PDRIIVGECRGGEALDLLQAMNTGHGGSMSTLHANSPIESLRRLETLALFSGEEIPLR
                     FLRAQVASAIDIVVQLQRLRTRRTVQSIAQVLPLTDSGEYAVREIYTYDYVNEGLKRV
                     GEASFEAEPHGSH"
     gene            1487..2095
                     /locus_tag="VFY27_00805"
     CDS             1487..2095
                     /locus_tag="VFY27_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5764078.1"
                     /translation="MKSTLLAITFAVPAGHALAQQEQLTDLTGQEVSVEQLVAALDIP
                     IRGVAAKCAPIQEQMTRLTRGVGSTPRTAEDVPSIKPMKTASISATFELNSDELTPDA
                     KSLLETVSKALNEPALSAQCFQLAGHTCDLGDDTYNLDLSQRRADAVKAFLVSHGVDE
                     NRLVTTGFGEMSPLVPNQDDQMRHKNRRVDLGALAPAALEYQ"
     gene            2124..>2393
                     /locus_tag="VFY27_00810"
     CDS             2124..>2393
                     /locus_tag="VFY27_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764079.1"
                     /translation="MNWPLSLVVFLGLVLLLAPLVYPGELSHVRPGLNRWLYDRAARS
                     YDRKWRSRAYSDPDVEAAVIAFAERSLAGSGVERVLDLGCGTGRGT"
BASE COUNT          500 a          725 c          741 g          427 t
ORIGIN      
        1 ccaggtcaca tcggagagaa cggcatcgct ggcggtgatc cacctcggat cggacgacgt
       61 actcacgcag atttacccga atccactcgg cttcacgccg caactggaag gcaatgcggc
      121 aaacggcgtc ggcggtgaaa tcggcctggc ggcgcgcgaa ccgataggcg tagagtggtt
      181 cgtattcatc gcgagcgaaa gtctgccact gcctcccacg atgcccggcc tgcaacaggt
      241 cggcagctgg gccaccgttt atccgctggg tgagcagggt tcgccaggcg agaagctggc
      301 gttgtggctt gcggacacct tcggcggcgc tggcgtctcc tcgaacatcc tgcagatcga
      361 agtcgtcgcc ggggagccgg caccgtgagc gccattccgc aggaaatctt caactcggcc
      421 ctcgaatatt tcctcgaccc gatcaaggag cttctcgcgg acgagagcgt gagggaaatc
      481 atgatcaatg gcgtcgggca aatctacatc gagaaaaaag gttcgctcga aaagagttcc
      541 attaccttcc ccgatgaaga ccagttgctg gccgccgtca ggaacattgc ccagtacgtc
      601 ggtcactaca ttcgaccggg tacggcgcgc ttcgacgcgc ggcttccgca gggtcatcgg
      661 gtgcacgttg tgcttccgcc tgtctgccgc cagggcatct cggtcacgat ccggaagcac
      721 accaagagcg cgttcggcct cgaagatctc gtgcggttgg gatccatcaa cgaggaatcg
      781 aaggattttc tccgcctgtg catggagcgc gagaaaaaca tggtcatttc cgggggcacg
      841 ggcacgggta aaacgacgtt catcaacgca ctttccgccc ttatccccaa aggtgaacgc
      901 atcattacga tagaagatgc cgcggaactg cagctcgacc aggaacacgt cgtctcgctc
      961 gaggcgcgcc cacccgaccg caagggagaa ggcgctgtga cgatccggga tcttctgcat
     1021 tcatcgctgc gcatgcggcc cgaccggatc atcgtcgggg agtgccgcgg aggcgaagcg
     1081 ctcgacctcc tgcaggcaat gaacaccggc cacggcgggt cgatgtcgac cctccacgcg
     1141 aattcgccga tcgagtcgct gaggcggctc gagacgctgg cactcttttc gggtgaagaa
     1201 attcccctgc ggttcctgcg cgcgcaggtt gcctcggcga tcgatatcgt cgtgcagttg
     1261 cagcggctcc gcaccaggcg aacggtccag agcatcgcgc aggtcctgcc gcttactgac
     1321 agcggtgaat acgcggtgcg tgagatatat acctacgatt acgtcaacga agggctcaaa
     1381 cgggttgggg aagcgtcctt cgaggccgaa ccgcacggca gtcattgaat tgcagacagc
     1441 agcgatacaa caggagataa aaacagtgaa tacaacacga acgatcatga aaagtaccct
     1501 gctggcgata acctttgccg tcccggcggg tcacgcgctc gcacagcagg aacagttaac
     1561 ggatttgaca gggcaggagg tttccgtcga gcaactggtt gccgcactgg acatcccgat
     1621 ccgcggcgta gcggccaagt gcgcaccgat ccaggagcag atgacgcgcc tgacccgcgg
     1681 tgtaggatcg acgccccgga ctgctgagga cgtaccttcc atcaagccca tgaaaacagc
     1741 cagcatctcg gccacgttcg aactgaattc cgatgagctt acgccggacg ccaagtcact
     1801 gctggaaaca gtctccaagg cactgaacga acctgcgctg tctgcgcaat gtttccagct
     1861 ggccggacac acctgcgacc tgggcgacga cacttacaat ctggacctgt cacagcggcg
     1921 ggccgatgca gtgaaggcct tcctcgtgag ccacggagtg gatgaaaaca ggctcgtgac
     1981 gaccgggttc ggcgaaatgt ctccgctcgt tcccaaccag gacgaccaga tgcgacacaa
     2041 gaatcgcagg gtggacctgg gcgcgctggc tccggcagca ctcgaatacc agtaacgcgc
     2101 cggcgctgca tcggcacttt gggatgaact ggccgctgag cctggtggtg ttcctggggc
     2161 tcgtactgct tctcgcgccg ctggtttacc cgggagagtt gtcgcacgta aggcctgggc
     2221 tcaatcgctg gctgtacgat cgcgccgcgc gcagctacga ccgtaagtgg cgctcccgcg
     2281 catacagcga cccggacgtt gaggccgcgg tcattgcatt cgcggagcga tcgctcgcag
     2341 ggtcgggggt cgaacgggtg cttgacctcg gttgtggcac cggtcgcgga aca
//
LOCUS       DASUOM010000039         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1070865,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000039 DASUOM010000000
VERSION     DASUOM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070865"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..2372)
                     /locus_tag="VFY27_00815"
     CDS             complement(54..2372)
                     /locus_tag="VFY27_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5764080.1"
                     /translation="MTELVLLALLGLAVTGLAWFWLNKRRLARRLAQLNMELVAAAGD
                     ASVGRRLSVAENGDIGGLARTINRLFDALGERDEEIQDRDRLFMDFARTLPEVVLVHD
                     ERILLANESAAALVGLTPDQLEGREVADLVKPAYRALFRNTTAKRLAGEDVPRRLEIQ
                     LINGDEQGLWVEAQSSSIEYRGTSAILTIARDVSYRKSLEVSLGRSKRQAQYTLESIA
                     EGVITTDNEGRIDYLNRAAEAMTGANREEATGHKIGEIFSLIDEADRRPLGDPVERCL
                     SMRRRVNMGRRALLVSRDGEHEHSIEITASPIRGPGNSISGTVVVFHDVSEIRGLTQQ
                     MSYHATHDALTGLKNRREFERRLQDAMDTAHAEEGVHMLLYMDLDRFKAVNDSCGHQA
                     GDNMLREVANLIKDKVRDSDYVGRLGGDEFGTLLISCPIEKARQIAADICTAVADYRF
                     VWQDKIFNIGISIGLVEISHSSGNLQDLMSAADSACYVAKQRGRGQVHVYSARDEAIA
                     RERGDIQWLRQMQAALHENRFELAKQPIIATGSGTGAPDDTGPAAEVLIRMPAEHGRS
                     TNSAEFLRPAERYQLMPQIDRWVVSAALAAIAAGEIKLPGRRNCSINISGETLADEGF
                     LSFVVEALDHSGVSPGSICFEVTESAISTNVQHAQRFIEVLHGIGCEFALDDFGNAFS
                     SLKHLPVDYLKIDGAYTRNVTTDQINQEMVSAMIKLARTMEFRVIAEQVERQEDFDWL
                     RDVGVDFVQGNFIEAPSMLGSGQTGAFRVLMP"
     gene            2711..3361
                     /locus_tag="VFY27_00820"
     CDS             2711..3361
                     /locus_tag="VFY27_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5764081.1"
                     /translation="MNTEFARQQMLGQQIRTFEVFDPKVLDVLAEVPREEFVPSAFRH
                     MAFADMQIPLPHGRFMMTPTVEGRLLQMLAIQPDDEVLEIGTGSGFLTACLARLGRTV
                     VSVDIFPDFPETAALKLKRAGIDNFTLASMDATRELPAGQFDAIAVTASCPIFDNRFL
                     QALKPNGRLFVIVGSPPVMEARLVVRGDSGRPKITSGFDTCVPPLINALAPPAFRF"
     gene            3452..>3657
                     /locus_tag="VFY27_00825"
     CDS             3452..>3657
                     /locus_tag="VFY27_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764082.1"
                     /translation="MKMFEEITPADLVARCRAGELWQLLDVREPWEVAIASVEQTVRM
                     PTKNIPMSEIPARHTELLESEPVAV"
BASE COUNT          678 a         1148 c         1077 g          754 t
ORIGIN      
        1 cgaagggagc cattccgtca aaggcaaccc cgacagatca gtcggctgat ccgttacggc
       61 atcagcaccc ggaaggcgcc ggtctgcccc gacccgagca tactgggcgc ctcgataaag
      121 ttcccctgta caaaatcgac ccccacatcc cgcaaccagt cgaaatcctc ctggcgctcg
      181 acctgctccg cgatcacgcg gaactccatc gtccgcgcca gcttgatcat tgcgctgacc
      241 atctcctggt tgatctggtc tgtagtgacg ttacgggtgt aggcgccgtc gattttcagg
      301 tagtcgaccg gcaggtgctt gaggctggaa aaggcgttgc cgaaatcatc aagggcgaat
      361 tcgcacccga tgccgtgcaa cacctcgatg aagcgctgcg cgtgctggac gttggtcgag
      421 atcgcgctct ccgtgacttc gaagcagatg gaaccgggtg aaaccccgct gtggtccagc
      481 gcttcgacga cgaagctcaa gaaaccctcg tcggccaacg tctcgcccga gatgttgatc
      541 gagcagttgc ggcggccggg caacttgatc tccccggccg cgatagcggc aagcgcggca
      601 ctgacgaccc agcggtcgat ctgcggcatc agctggtagc gttccgcagg acgcaggaat
      661 tcggccgaat tggtggagcg tccatgttcg gcgggcatgc gaatcaatac ttcggccgcc
      721 ggtccggtat cgtcaggagc tccggtgccg cttccggtgg caatgatggg ctgtttcgcc
      781 agttcgaagc gattttcatg gagggcggcc tgcatctgcc gcagccactg gatgtcgccg
      841 cgctcgcggg ctatcgcctc gtcgcgcgcg gaatacacgt gcacttggcc gcggccgcgt
      901 tgcttggcga cgtaacaggc agaatcggca gcactcatga ggtcctgcaa gttaccgctg
      961 gagtgactta tctcaacaag gccgatcgaa atgccgatgt tgaagatctt gtcctgccag
     1021 acaaatcggt agtccgctac cgcggtgcag atatccgcgg cgatctgcct ggccttctcg
     1081 atcgggcagc ttatcaacag agtgccgaac tcgtcaccgc cgagccgccc gacataatcc
     1141 gaatcccgca ccttgtcctt tatgaggttg gcgacctccc ggagcatatt gtcgccggcc
     1201 tggtgaccgc agctgtcgtt gaccgccttg aaccggtcaa ggtccatgta caggagcatg
     1261 tgcacgcctt cctcggcgtg cgcggtatcc attgcgtcct gcaggcgccg ctcgaattcg
     1321 cgccggttct tgagaccggt aagtgcatcg tgcgtggcgt ggtagctcat ctgctgcgtc
     1381 agtccgcgga tctcgctgac gtcgtgaaat accaccacgg ttccggatat gctgttgccg
     1441 gggccgcgaa tcggagatgc ggtgatctcg atcgagtgct cgtgttctcc gtcgcggctg
     1501 accagcaacg cgcgccgacc catgtttacg cgccggcgca tcgaaaggca gcgctcgacc
     1561 gggtcgccga gcggtcggcg atccgcttcg tctatgagtg agaagatctc gccgatcttg
     1621 tgaccggtag cctcctcgcg gttcgctccg gtcattgctt cggcggcccg gttcaggtag
     1681 tcgatccggc cctcgttgtc cgtcgtgatg acaccttccg caattgactc cagggtgtac
     1741 tgggcctgcc gcttgctgcg gcccagggaa acttcgaggc tcttcctgta gctgacgtca
     1801 cgagcgatgg tcaggatggc gctggtgcca cgatactcga ttgacgagct ctgcgcctcc
     1861 acccacagcc cctgctcgtc cccgttgatc agctgtatct cgaggcgccg cggcacgtct
     1921 tcgccggcca gccgcttcgc cgtggtgttg cgaaacagcg cccggtaggc aggcttgacg
     1981 aggtcagcga cctcccgtcc ttccagctgg tcgggagtca ggccaaccag cgcggccgca
     2041 ctctcgttcg caagcaggat ccgctcgtcg tggaccagca cgacctccgg aagggttcgg
     2101 gcgaaatcca tgaaaagccg gtccctgtcc tggatttcct catcgcgttc accgagtgcg
     2161 tcgaacagcc tgttgatggt tcgtgccaga ccaccgatat cgccgttctc cgcgaccgac
     2221 aagcgccggc ctaccgacgc gtcgccggcc gccgccacga gttccatatt gagttgggcc
     2281 aggcgccgcg caagacggcg cttgttcagc cagaaccaag cgagccccgt caccgccagc
     2341 ccgagcagtg cgaggagaac cagttcggtc atctgcgcct tcctgcgccg acagattggg
     2401 agcgcgttcg acgctttatt tttgaattat tgcagcgcaa gttctcgtta tcccgttccg
     2461 gctatcgcgc caatgatgcc gtgaaacgat catttgttat tattggccgc tccgggcgtg
     2521 tccccaatgc cggggtcacc acctgaacca gagcgttgat aatgttcact gtcaagcata
     2581 tcccagcggc aacggcgcac tgcgccgatt caccataatt cacgatgcag gacgggttgc
     2641 acgcttgagg gtttgcgggc ggtcaaaaag acacaggctc actaaagcta ctttatcggg
     2701 atcagactga atgaacactg aatttgcccg tcagcagatg ttgggccagc aaataagaac
     2761 cttcgaagtc tttgacccta aagttctcga cgttctggcg gaagtgcctc gcgaggaatt
     2821 cgtgccgtcc gcattccgcc acatggcctt cgcagacatg caaatcccct tgccgcacgg
     2881 acggttcatg atgacgccga cggtcgaggg gcggctgttg cagatgctgg cgatccagcc
     2941 tgatgacgag gttctcgaaa ttggtacggg ctccgggttt ttgaccgctt gcctcgccag
     3001 actcggtcgg accgtggtca gcgtcgacat attccctgat ttcccagaaa ccgcggcact
     3061 gaagctcaag cgcgcaggca tcgataactt cacgctggcg tcgatggatg ccactcgcga
     3121 gcttcccgcc ggccagttcg atgccattgc cgtgacggct tcctgcccga ttttcgacaa
     3181 tcgctttctt caagcactca aaccgaatgg ccggctgttc gtgatcgtgg gcagcccgcc
     3241 ggtgatggaa gcgcggctgg ttgttcgcgg cgacagcggc aggcctaaga tcaccagcgg
     3301 tttcgatacc tgcgtcccgc ccctgatcaa tgctctggcg ccgcctgctt tccggtttta
     3361 gaaccgtggt gaaaccactg ttacgcttgc ggcatctaaa tgaggtatca cgccacccgt
     3421 tcccgccgag ccggcctgga aacgtcgttg aatgaagatg tttgaagaaa taactcccgc
     3481 cgacctggtg gcgcgctgtc gtgccggcga actttggcag ctcctcgacg tccgagaacc
     3541 ctgggaagtg gcgatcgcct ctgtagaaca aacggtcagg atgccgacaa aaaacatacc
     3601 catgtccgaa atccctgccc gccacaccga attgctggag tccgaaccgg tcgccgt
//
LOCUS       DASUOM010000040         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_106209,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000040 DASUOM010000000
VERSION     DASUOM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106209"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VFY27_00830"
     CDS             complement(<1..1716)
                     /locus_tag="VFY27_00830"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX5764083.1"
                     /translation="MSAPKSPPAAPRLRSRAWFDDASNVDMTALYLERYMNFGLSLEE
                     LQSDRPVIGIAQTGSDLAPCNRHHLVLAERVREGIREAGGIVLEFPVHPIQETGKRPT
                     AGLDRNLAYLGLVEVLFGYPLDGVVLTIGCDKTTPACLMAAATVNMPAIALSVGPMLN
                     GWFKGERTGSGTIVWKARELLAAGEIDYPGFVKLVASSAPSTGYCNTMGTATTMNSLT
                     EALGMSLPGSAAIPAPYRDRQECAYLTGKQIVEMVAADRKPSDIMTRDAFVNAIRVNS
                     AIGGSTNAPLHLIAVARHIGVELTIDDWQELGLDIPLLVNLQPAGEYLGEDYYHAGGV
                     PAVVAQLMQNGLIAESALTVNGRSIGENCRGALIEDENVIRPFDRPLKDKAGFTVLRG
                     NLFDSAIMKTSVISEQFRARYLSNPEDPDAFEGTALVFEGPEDYHSRIDDPSITIDEQ
                     TILFMRGTGPVGYPGAAEVVNMRPPANLIVAGVHNLPCIGDGRQSGTSGSPSILNASP
                     EAAVGGGLALLKTGDRVRIDLRRGEANMLIGDEELEARRRALKEAGGFPYPESQTPWQ
                     EIQRAT"
     gene            complement(1760..2923)
                     /locus_tag="VFY27_00835"
     CDS             complement(1760..2923)
                     /locus_tag="VFY27_00835"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX5764084.1"
                     /translation="MPRVIKSMKNLVVTFVLATLNLFLAGLPAYPAEATRMDFGELKD
                     GTGIEAIVLSNSNGTSATIITLGATIQSLSVPDRDGRSEDIVLGYRTADEYLENPQYF
                     GATVGRYANRIAGGRFTLDGKHYTLPKNDGTNHLHGGPQGLDKALWKVESVSKGSPAT
                     VTFSHSSPDGEGGYPGTLDITATYALSDQDELSIEYRATSDKPTIVNITNHAYFNLAG
                     EAGRLDVMDHRLTLLADRYTPVDETLIPTGELREVEGTAFDFREGEPIGLRIRDGGEE
                     QIRFGRGYDHNFVVNDAAGTLRPAARVEDPHSGRVMEVMVTAPGVQFYSGNFLDGTTV
                     GKNGRVYRQGDAFCLEPQVFPDSPNKPRFPSARLDPGDTYVNRMVFRFSTTGR"
     gene            3136..>3359
                     /locus_tag="VFY27_00840"
     CDS             3136..>3359
                     /locus_tag="VFY27_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764085.1"
                     /translation="MVLRQQGFLASLAVSALIAGTSCSTAWAAEDALYKSPGAAVPAR
                     VDDLLGRMTLEEKIVQLLAVWEDKAEILDDS"
BASE COUNT          596 a         1053 c         1051 g          659 t
ORIGIN      
        1 ggtggcgcgc tggatctcct gccacggcgt ctggctttcc ggatagggga agcctcccgc
       61 ttccttgagc gcccgcctcc gcgcctccag ttcctcgtcg ccgatcagca tgttggcttc
      121 accgcgacgc agatcgatac ggacccggtc ccccgtcttc agcagtgcaa gtccgccccc
      181 gaccgctgct tccggagaag cgttcaggat cgagggcgaa ccggatgtgc cggattgcct
      241 tccgtcaccg atgcagggaa ggttgtggac gcccgccacg atcaaattgg ccggcggacg
      301 catattcact acctccgcgg ccccggggta accgaccggg ccggtgccgc gcatgaagag
      361 gatggtctgc tcatcgatcg tgatggaggg atcgtcgatg cgcgagtgat agtcctccgg
      421 cccctcgaac acgagcgccg taccctcgaa cgcgtccggg tcctccgggt tcgagagata
      481 tcgggcccgg aactgttctg aaataacgct ggttttcatg atggcggaat cgaacaggtt
      541 gccgcgcagc accgtaaagc ccgccttgtc cttgagcggg cggtcgaacg gccggatgac
      601 attctcgtct tcgataagcg cgccgcggca attttcgcca atggagcgcc cgtttaccgt
      661 cagcgccgat tccgctatca atccgttctg catgagctgt gcgaccacgg ccggcacgcc
      721 gccggcgtga taataatcct cgcccaggta ctcgcccgcc ggctgaaggt tcaccagcaa
      781 cggaatgtcc agccccagtt cctgccagtc gtctatggtc aactcgacgc cgatatgccg
      841 cgcgacggca atgaggtgga ggggcgcatt ggtcgagccg ccgatggcgg agttgacgcg
      901 gatcgcgttg acaaacgcgt ccctcgtcat gatgtccgac ggctttcggt ccgcggcaac
      961 catctcgacg atttgtttgc ccgtaagata cgcgcactcc tggcggtcgc ggtagggcgc
     1021 cggtatcgcg gcggaacccg gcagcgacat cccgagcgct tcggtcaggc tgttcatcgt
     1081 ggtggccgtg cccatcgtat tgcagtagcc ggtggaaggc gcggacgagg cgaccagctt
     1141 cacgaaaccc gggtaatcga tttctcccgc cgccagtaat tcccgggcct tccacacaat
     1201 ggttcccgag ccggtgcgtt cgcccttgaa ccagccgttc agcatcgggc ccaccgacag
     1261 cgcgatcgcc ggcatgttca cggtcgcggc ggccatcagg catgcaggcg tggtcttgtc
     1321 gcacccgatc gtcagcacga ccccatccag cggataaccg aacagcactt cgacgagccc
     1381 gaggtatgcg agattccggt cgaggcccgc agtcggccgc ttccctgttt cctgaatcgg
     1441 gtggacggga aactcgagca cgatgccgcc tgcttcgcga atgccttccc gcacccgttc
     1501 cgcgagaacc aggtgatggc ggttgcatgg cgcgaggtca ctgcccgtct gggcgatccc
     1561 gatgaccggc ctgtccgact gcaattcctc cagcgacagc ccgaagttca tgtagcgctc
     1621 gaggtacagc gcagtcatgt ccacgttgga ggcatcgtcg aaccacgcgc gtgagcgaag
     1681 acgtggcgcg gccggtggtg atttcggtgc actcataggc aaatttcgga cattgacgga
     1741 atttcggggt gcgcgggctt caccggccgg tggtcgagaa gcgaaacacc atcctgttca
     1801 cgtacgtatc gccgggatcg aggcgcgcgg atggaaagcg gggtttgttc ggggaatcgg
     1861 gaaacacctg gggctccagg caaaacgcat ctccctgccg atagactcgg ccattcttcc
     1921 cgacggtcgt gccatcgaga aaattgcccg aataaaactg cacgccaggc gcggtcacca
     1981 tcacttccat cacgcgtccc gaatgcgggt cttcaacgcg cgcggccggg cgcaacgttc
     2041 cggcggcatc gttgacgacg aaattgtggt cgtatcccct cccgaagcga atctgctctt
     2101 caccgccatc gcggattcga agcccgatgg gctcgccctc gcgaaagtcg aaggcggtgc
     2161 cttcgacctc ccgcaactcg ccggtaggaa tcagcgtttc atcgacgggc gtgtagcgat
     2221 ccgcaagcaa tgtcaggcga tgatccatga cgtcgaggcg ccctgcctcg cccgccaggt
     2281 tgaagtaggc atggttcgtg atgttgacga tcgtcggctt gtcgctggtc gcgcgatatt
     2341 cgatggaaag ttcgtcctgg tcgctgaggg cgtaggtggc agtgatgtcg agcgtcccgg
     2401 gatagccccc ttcgccatcg gggctggaat ggctgaaagt gaccgtggcc ggtgagccct
     2461 tgcttacgga ttcgactttc catagcgcct tgtccagtcc ctgcgggccg ccatgcagat
     2521 ggttggtgcc gtcgttcttc ggcagcgtgt agtgctttcc atcgagggtg aagcggcctc
     2581 cggcgatgcg attcgcgtag cggccaacgg tcgcgccgaa gtactgggga ttttccaggt
     2641 attcgtccgc cgttctgtag ccgagcacga tgtcctcgct cctgccgtcc cggtccggca
     2701 ccgacagcga ctgtatcgtc gcaccgagcg tgatgatcgt ggcgctcgtg ccattcgaat
     2761 tcgacagcac gatcgcctct atgccggtgc cgtccttcag ctcaccaaaa tccatacgcg
     2821 tcgcttccgc cgggtacgcc ggcaggccgg cgaggaacag gttcaaagtg gcaagaacaa
     2881 acgtcacgac caggtttttc attgatttta tgaccctcgg catgtccttg ctcggccgcc
     2941 tgtcaatact cggacaaaat ggcaccaaat cgtcccgctg acaaggcccg tgcgaatcgc
     3001 attgacaggc aaagcggtgg cgcctatggt agcgctatca cggctgccac gggtcctgga
     3061 aagccagcca gtggggaagc tctgagttcg aggcccgccc gcgggcacgc cagcggcaac
     3121 tacaggggga tttttatggt tcttcggcag caagggtttc ttgcgtcgtt ggcggtttcc
     3181 gctttgatcg ccggcacgtc gtgctccacg gcgtgggccg cggaggacgc cttatataag
     3241 agtcctggcg ccgccgtccc agcgcgtgtc gatgacctgc tcgggcgcat gaccctcgag
     3301 gagaaaatcg tgcagctgct ggcggtctgg gaggacaagg ccgaaatact ggatgactc
//
LOCUS       DASUOM010000041         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_844988,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000041 DASUOM010000000
VERSION     DASUOM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844988"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFY27_00845"
     CDS             complement(<1..578)
                     /locus_tag="VFY27_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5764086.1"
                     /translation="MVYDLGGFVLDPRRRSLSRSDGEPIRVTAKAFDTLIYLVEHAGE
                     LVSRSEIIDVIWPRTIVEEGNLNQTIYALRRALGDHGGHRYIATVPGRGYQFVAEVQT
                     IRSDPRANSQPDSGPVPAQLAPSPRWSTMALVAGAVIGVSLVGAYSITRTPYGGSQGL
                     APVSRVSPVTTYPGDELSPSLSPDGRQVVFSW"
     gene            789..2054
                     /locus_tag="VFY27_00850"
     CDS             789..2054
                     /locus_tag="VFY27_00850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX5764087.1"
                     /translation="MHNPLRKPRKSNFPALILLCGIGILSLPLRAEVVSDWNATAVTV
                     VNDPTLGSLGRQIPYLSMVHAAIYDAVNGIDRRYTVLGVSPTSDTRGASKQAAAASAA
                     YHLLLELFPAQQSLLDSAYTNSLSAIRDGIAKTKGIAIGQEVAAAWIELRDGDGRDLP
                     APPYIPGTEPGDYQLTGPPPILTWLGGMRPFTLKYASQFRAYGPPDLTSHQYARDLNM
                     VKKLGSATSTERTAEETEIAMFHTENPNIFWWRNFRDLAAAMRLDTAESARFFALLVI
                     GQGDAIQACFDSKYHFNFWRPLTAVPAADLDDNPATKADPAWSPLAPTPPHPEYPAAH
                     ACAAGLTAEVLEHFFGKKHFVMTFRSTVPGSVPHTFHSANDLVEEIIMARVYGGMHFA
                     TSGVHGAVLGRRVGQWITSKYFLPVKRPE"
     gene            complement(2116..3003)
                     /locus_tag="VFY27_00855"
     CDS             complement(2116..3003)
                     /locus_tag="VFY27_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5764088.1"
                     /translation="MPELPDIKVYVDALRDRIQGHTLERMRINNPFVVRSTSPMPSEV
                     PGLTVTSVDHQAKRIVISLEQDVRIFIHLMIAGRLHWKDRGAPLKGRNQLAALDFDSG
                     SLVLTEAGTKRRASIHLARGAMASAEFDRGGLDPLLITEAQFRERLSKENHTLKRALT
                     DQRLLAGIGNAYSDEILLAARLSPFKQTQALTDDEWQRLYAATRDTLNLWIERLRREA
                     GSGFPKKVTAFREGMAVHGRYRKPCPVCGSPVQRIVYAENEANYCARCQTGERLLADR
                     SLSRLLKDNWPKRLEDLEE"
     gene            3100..>4018
                     /locus_tag="VFY27_00860"
     CDS             3100..>4018
                     /locus_tag="VFY27_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX5764089.1"
                     /translation="MRLVEQLLRLSATDLANHLSCAHLTRRSLAVAEGRARKPKWQDP
                     IADILRERGIEHEAAYLAHLRETEGLDVVAIPEGSDADSFDRTRAAMQEGAPLIYQAS
                     LGNARWHGRADFLRRVEKPSRLGSWSYEVIDAKLATITRAGTILQLCVYSELVAELQD
                     AWPEYAYVVAPHHGFKPEPYRLADFLAYYRLVKRRLEHALGSNADAYPEPVMHCEVCA
                     WWQLCNRVRRDDDHLGFVAGISRQQIKELRNIGVNTLEHFGDLREVPKPARGSREALA
                     RKRDQAAIQLKARRLDVPQFEILEPQDAEH"
BASE COUNT          766 a         1300 c         1218 g          734 t
ORIGIN      
        1 tcccaggaaa agactacctg gcggccgtcc ggcgacaacg acggcgaaag ctcgtctccg
       61 gggtacgtcg tgactggcga gacgcgcgag accggcgcga gaccctgcga gccaccgtac
      121 ggagtccggg tgatgctata ggcgcccacc agcgagaccc cgatgactgc gccggcgaca
      181 agtgccatcg tcgaccagcg cggcgaaggc gcgagttgtg ccgggactgg tccggaatcc
      241 ggttgcgagt tagcgcgcgg gtccgagcgt attgtttgta cctcggcgac gaactgatag
      301 ccgcggccgg gaacagtggc aatatagcga tggccgccat ggtctcctag cgcccggcgc
      361 agcgcgtaga tcgtctggtt caaattgcct tcctcgacga tcgtgcgcgg ccaaataaca
      421 tcgataatct cggaccggga caccaactcg cccgcgtgct cgaccaggta gatcagcgtg
      481 tcgaaagctt tcgctgtgac cctgatgggc tcgccgtccg agcggctgag cgagcgacgt
      541 ctcgggtcca gaacaaaccc gcccagatcg tagaccaacc acggccctga ctcgctcatc
      601 gccgtcggca ctgccatcac cccggtggcg tcacataaga attaataccg tttgatattg
      661 ccgcaaagct ttttgatacg cggctgatta gtttactgct gaaactcgtc cgccttggcg
      721 aaataaaact gttacgaaac aaggcagtgt cgttcacccc ccaatcaacc ctcacctgga
      781 gaaatccaat gcacaaccca ttgcgcaagc cgcgaaagtc gaacttcccg gctttgattc
      841 tgctctgcgg catagggatc ctttccttgc cgctcagggc cgaggtcgtc agcgactgga
      901 atgcaacggc cgtcacggtc gttaacgacc cgacgctagg ctcccttgga cgccagatcc
      961 cctatctgtc catggtccac gcggcgattt acgatgccgt caacgggatc gatcgccgct
     1021 ataccgtcct gggagtcagt ccgacgtcag atacgcgcgg cgcctctaag caggcggccg
     1081 ccgcttccgc ggcctatcac ttgctgctcg aactctttcc cgcgcagcag tccttgctcg
     1141 attccgccta cacgaactcg ctctccgcga ttcgcgacgg gatcgccaaa accaagggca
     1201 tcgcgattgg ccaggaagtg gcggcggcct ggatcgagct acgcgacggc gacgggcgag
     1261 atttaccggc acctccctac ataccgggca cggagccggg cgactaccag ctgaccggcc
     1321 cgccgccaat cctcacgtgg ttaggcggta tgcggccatt taccctcaag tatgcatccc
     1381 agtttcgggc ttacggtcct ccggacctta cgagtcacca gtacgcgcgc gacctgaaca
     1441 tggtcaagaa actcggctct gcaaccagca ccgaaagaac tgccgaagaa accgagatcg
     1501 caatgttcca tacggagaac ccgaatatct tctggtggcg taatttccgc gatctggcag
     1561 cggcgatgcg tctcgatacc gcagaaagcg cgcgtttttt cgcactgctc gtgatcggcc
     1621 agggcgatgc gatccaggcg tgcttcgact cgaagtatca cttcaacttc tggcgccctt
     1681 tgacggctgt accggctgcg gatctcgacg ataatcctgc gacgaaagcc gacccagcgt
     1741 ggtcacccct cgcaccgaca cccccgcacc cggagtatcc ggcggcgcac gcctgtgcag
     1801 ccggtctcac cgccgaggtg ctggagcact tcttcggcaa aaagcatttt gtgatgactt
     1861 tcagaagtac tgtccccggc tcggtacctc acacgttcca ctcggccaat gatctggtcg
     1921 aggaaatcat catggcacgc gtctacggcg ggatgcattt cgcgacgtca ggcgttcacg
     1981 gtgccgtatt gggcagaagg gtgggccagt ggattaccag caagtacttc ctcccggtga
     2041 agcggcccga ataacatttg caggttccgc gcgggcgctt gtgcccgcgc gggccggcgc
     2101 gcgaatcagc ggcaactact cttccaggtc ttcgagcctc ttcggccagt tgtccttcag
     2161 gagtcgcgac agcgagcgat ccgcgagcaa tcgctcgccg gtctggcagc gggcgcaata
     2221 gttcgcctcg ttctccgcgt agacgatgcg ctgcacgggc gagccgcaga ccgggcaggg
     2281 tttgcggtag cggccatgca cggccatgcc ctcgcggaag gcggtgacct tcttcgggaa
     2341 accgcttccc gcctcccgcc gcagccgttc gatccacaga ttgagcgtgt ccctggtcgc
     2401 ggcatacagg cgctgccact cgtcatcggt cagtgcctgc gtctgcttga acggcgacag
     2461 cctcgccgcc agcaggatct cgtcggagta cgcgttgccg atgcctgcaa ggagccgctg
     2521 gtcggtcagc gctcttttca aggtatggtt ctccttcgac aggcgctcgc gaaattgcgc
     2581 ctccgtgatc aggagcgggt cgaggccgcc gcgatcgaac tccgccgatg ccatcgcacc
     2641 gcgggccaga tgtatggagg cgcgccgctt cgtaccggcc tccgtgagca ccagcgaacc
     2701 gctgtcgaaa tccagggccg ccagctgatt gcggcccttc agcggcgccc ctctgtcctt
     2761 ccagtgcagc cgcccggcga tcataaggtg gataaagatg cggacgtcct gctcgagcga
     2821 gatgacaatg cgctttgcct ggtgatcgac actcgtcacg gttaaacccg gcacctccga
     2881 cggcatcggc gatgtgctgc gcacgacaaa gggattgttg attcgcattc gctctagcgt
     2941 gtgcccctgg atccgatcac gcagggcatc gacgtagact ttgatgtcgg ggagctctgg
     3001 cacacgttca tgctgcagca aacccgccgt tctggccaga tgcccggcgg cattcccgca
     3061 ccggggaaca ctgaagcctc acctgcgtat actcccggca tgcgtttagt cgaacagctc
     3121 ctgcgcctgt ccgccacgga tcttgcgaac catctgagtt gtgctcacct gacccggcga
     3181 agccttgctg tagcggaagg gagggccagg aaaccgaagt ggcaggaccc gatcgccgac
     3241 atcctccgcg agcgcggcat cgagcacgaa gctgcctacc tggcacatct cagggagacg
     3301 gaagggctcg acgtggtagc gatccccgag ggcagcgatg ccgacagctt cgacaggaca
     3361 cgcgccgcga tgcaggaagg tgcgccgctg atctaccagg catccctcgg caacgcgcgc
     3421 tggcacgggc gcgcggactt cctgcgccgg gtggaaaagc ccagccggct tggttcatgg
     3481 tcctacgagg tgatcgacgc gaagctcgca accataacgc gggctggcac gatcctgcag
     3541 ctgtgcgtgt actcggaact ggtcgccgaa ctgcaggatg cgtggcccga gtatgcgtac
     3601 gtcgtcgcgc cgcatcatgg tttcaagccg gaaccttacc ggctagcgga cttcctggct
     3661 tactaccggc tcgtcaaacg caggcttgag cacgccctgg gaagcaacgc ggacgcctat
     3721 cccgagcccg tgatgcattg cgaggtctgc gcgtggtggc agctttgcaa ccgggtccgg
     3781 cgcgacgacg accatctcgg tttcgtcgcg ggcatcagcc gccagcagat caaggagctt
     3841 cgaaacatcg gtgtgaacac cctcgagcat ttcggcgacc tgcgcgaagt gccgaagcct
     3901 gcgcgcggtt cgcgagaagc gctggcccgg aagcgcgacc aggcggcgat acagctgaag
     3961 gcgcggcggc tcgacgttcc acagttcgaa atcctggagc cacaggatgc cgagcacg
//
LOCUS       DASUOM010000042        11631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_826510,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000042 DASUOM010000000
VERSION     DASUOM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826510"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFY27_00865"
     CDS             complement(<1..374)
                     /locus_tag="VFY27_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5764090.1"
                     /translation="MVASTADSEHRSTKSPALGLRLLLLAALSVVLLVVDHRENHLDA
                     VRKAVGTAVYPLRILVDAPVSTWQWIRDNTASQNDLLLEIGRLNAERLLTHARLQRFT
                     ALESENARLRAMLDATSKVRDQV"
     gene            complement(472..1515)
                     /locus_tag="VFY27_00870"
     CDS             complement(472..1515)
                     /locus_tag="VFY27_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5764091.1"
                     /translation="MLKNILGYFSNDLSIDLGTANTLIYSRGHGIVLDEPSVVAIRED
                     SGRGGRVVEAVGAEAKNMLGRTPGNITAIRPLKDGVIADFTVTEKMLQHFIRKAHGKR
                     FFRPSPRVLVCVPYGSTQVERRAIRESAEGAGARKVLLIPEPMAAAIGAGMPVHEARG
                     SMVLDIGGGTSEVAVISLNGIVYAASVRIGGDRFDEAITNYVRRNYGILIGEATAERI
                     KHEIGSAFPGQEVREISVKGRNLSEGVPRSFTLNSNEILEALQEPLQGIVGAVKEALE
                     QTPPELGADVAERGIVLTGGGAMLRDIDKLLMEETGLPVIIADDPLTCVARGGGRVIE
                     LIDEHGPSVFGLE"
     gene            1659..1943
                     /gene="gatC"
                     /locus_tag="VFY27_00875"
     CDS             1659..1943
                     /gene="gatC"
                     /locus_tag="VFY27_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5764092.1"
                     /translation="MSLTKDQVHHIALLARLKLEESEFDDVVDKLSRIVDFVDQLQAA
                     PTEGVVPMAHPLDQSQRLRPDVVTETIDRDAIQANAPLARDGFYLVPKVL"
     gene            1968..3422
                     /gene="gatA"
                     /locus_tag="VFY27_00880"
     CDS             1968..3422
                     /gene="gatA"
                     /locus_tag="VFY27_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5764093.1"
                     /translation="MHVLTLTELARGIREGDFSALELTETLLRRIDAHNFDLNAFITV
                     TAEQALASAEAADKARAAGNAPVLNGLPIVHKDLFCTSGILTTCGSRMLSNFVSPYDA
                     TVVARMKSAGAVTLGKTNMDEFAMGSSNETSWFGPVANPWDPARSPGGSSGGSAAAVA
                     ARLVPAGTGTDTGGSIRQPAALTGTVGIKPTYGRVSRYGMVAFASSLDQAGVITRSAE
                     DAALLLGVMAGFDERDSTSVDQPVPDYLAALGTSLKGLKIGIVRQHFDEGLDPQCAAR
                     IREALEVLRAEGATLVEVDLPNLELSVPTYYVVAPAECSSNMSRFDGVRFGHRAADPE
                     DLLDLYCRSRGEGFGAEVKRRIMTGTYVLSAGYYDAYYLKAQKVRQLIGDDFRKAFGQ
                     VDVIAGPTAPTPAFALGEKTDDPITMYLNDIYTIGANLAGNPGISLPCGFAEKLPVGL
                     QLVGPHFSEEKLLNVAHQYQLLTDWHKACPEAFQ"
     gene            3419..4882
                     /gene="gatB"
                     /locus_tag="VFY27_00885"
     CDS             3419..4882
                     /gene="gatB"
                     /locus_tag="VFY27_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5764094.1"
                     /translation="MSVPAAHSRAASEWEAVIGLEIHMQIATASKIFSAAPTTYGAEP
                     NTQASLIDLGYPGVLPVLNEDVVRMACKFGLAVNASIAPRSVFARKNYFYPDLPKGYQ
                     ISQYELPIVARGELTILDEQGREKRIGITRAHLEEDAGKSLHSGFDEFTRIDLNRAGT
                     PLLEIVSDPDLRSAKEAVAYMRKVHTLVRYLEISDGNMQEGSFRCDANVSIRRCGDQT
                     LGTRTELKNLNSFRFVERAINFEIERQQEVLEEGGTVLQETRLYDSELDETRPMRTKE
                     EANDYRYFPDPDLLPVVIEEAFIESVRETLPELPDAKRQRFVEQYDIRAGDAEILTTS
                     RGLANYFEATAASSDPKLAANWILGDLSAALNREGLEIDDSRIPPEGLAELVARIADS
                     TISGKIAKEVFDAMWAGEGTASGIIDVRGLRQITDPSAIEAVVGKVIEANPGQAADYR
                     SGKDKLLGFFVGQVMKETSGKANPAEVNRILKERLKS"
     gene            4882..5754
                     /locus_tag="VFY27_00890"
     CDS             4882..5754
                     /locus_tag="VFY27_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX5764095.1"
                     /translation="MAAHDVVMPFVFEALPVRGAIIQLQKSWHRMLLGHNYDQPVLET
                     LGHSAAASALIAQSLKFDGSITLQINGNGPLAMLVMQCTSNLELRGMASAGQLDDDIA
                     FSELVRRARCAITVNAGAMERPYQGIIEVSGDSLADSLEDYYRRSAQIPTHLRLVSDR
                     NCCGGILLQQMPGEQSPVADDWRRVGLLAGTLRIHDLADGLAPSLLGRLFAEDDLRVF
                     RPREAQFRCRCSRRRSEDVLKLLGEQDTRDLLAEQGRIEVTCEYCGRTRTFDSIDISQ
                     LFTEPPVATSDTLH"
     gene            5870..6760
                     /locus_tag="VFY27_00895"
     CDS             5870..6760
                     /locus_tag="VFY27_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5764096.1"
                     /translation="MKPGNERLLLQFKILADPLRLRLVALCRRGECSVSELTQAVGQS
                     QPRVSQHLKKLVEAGLLERFRDGKRVYYRVPAAEGHAARRLLDMLPDAEPLMLEDAGR
                     LAAMRAQRGFTHPVSDAGVGTANRAMHRLLLELTVTAPVGNLLDIGCGQGRVLKLLAS
                     RANRAIGVDIDPQARLTARTDLMLAGLPNCSLRHGDMYRLPFADGEFDTIILDDVLAD
                     AVDPVDALQEAKRLLHPNGRLLVLQALRGQPVMALQKSLAEWSRAAALRLAPARFAPE
                     KTPEWLLCVATRADANAAAA"
     gene            6783..7667
                     /gene="metF"
                     /locus_tag="VFY27_00900"
     CDS             6783..7667
                     /gene="metF"
                     /locus_tag="VFY27_00900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5764097.1"
                     /translation="MNDSRTATQVSFEFFPPKTEEMEKTLWESIERLSSLSPRFVSVT
                     YGADGSTRERTHAAVARLLKDTSLTAAPHLTCVGASRGEIDDIARGYWDMGVRHLVAL
                     RGDPPKAADVPWQAYAPHPDGYAYASDLVAGLKKVADFDISVAAYPEVHPEAQDAGTD
                     LENLKRKIDAGASRAITQFFFDVDTYLRFRDRCAARRIETAIVPGILPITRFPQLQRF
                     AEQCGASVPEWLHERFQGLEDDPETRRLIAASVAIEQVRQLKKEGINEFHFYTLNRSE
                     LTYAICHALGVRAEQAAA"
     gene            7686..11393
                     /gene="metH"
                     /locus_tag="VFY27_00905"
     CDS             7686..11393
                     /gene="metH"
                     /locus_tag="VFY27_00905"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5764098.1"
                     /translation="MQREQRIELLKKLLAERILLLDGAMGTMIQAYRLSEDDYRGERY
                     REWPRDLKGNNDLLSITRPEVIRDIHRQFLEAGADIIETNTFNSNAPSMGDYGMETLV
                     SDLNRAAASLARETADSFAERSGEPKFVAGVLGPSNRTASISPDVNDPGFRNITFERL
                     AETYQVAATALIEGGVDFLMIETIFDTLNAKAAIFGLRRSFEAAGFELPVMISGTITD
                     ASGRTLSGQTTEAFWNSVRHAKPFMVGLNCALGAADLRPYVAELSRIADTGVSAHPNA
                     GLPNEFGEYDETPEEMAGIIVEFASSGLVNLVGGCCGTTPDHIRRLREAIAGKAPRNI
                     PSVPVRCRLSGLEPLNLGPDDLFVNVGERSNVTGSKLFRRLIEAGEYGEAVQIARQQV
                     ENGAQIIDVNMDEGMLDSEKAMQTYINLISSEPDVARVPVMIDSSKWTVIETGLRCVQ
                     GKSIVNSISLKEGEEAFIRQARLVRDYGAAVVVMAFDEQGQADSVARKVAICKRCHDI
                     LTKQVGFDPADIIFDPNIFAVATGIEEHNRYGLDFVEATRAIKEALPHVMVSGGVSNV
                     SFSFRGNDVVREAIHSVFLYHAIKAGMDLGIVNAGQLAIYEDLPKDLRDAVEDVILAR
                     RDDATERLLEVAESYKGLAGGQKAKEKDLGWRDLPVNKRLEHALVNGIDEYVIEDTEA
                     ARLAAQKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVNHLVPFIEKE
                     KGGARKSNGRIVMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVPCEKILETAKRE
                     NAQIIGLSGLITPSLDEMVHVASEMQRLKFQLPLLIGGATTSPAHTAVKIEPNYEGPV
                     IYVKDASRSVGVAQALVETASRDELITRTRRENATRRERHAGKTRLAPQLSLSQARER
                     KHRCDWVNYVPPRPSFSGTRVLDKVDLATLRDHIDWMPFFNAWEFHGKFPAILDDPSV
                     GSSVRPLYADANAMLDRIIAEGWLEARAVFGFFPASTVDDDDILVYENDSRQKSLLRL
                     CHMRQQRGKPSGMAQDCLADFVAPAGSGIDDYIGAFAVTAGIGIDEHVQRFEADHDDY
                     SSIILKALADRLAEALAEYLHLRVRREFWGYDPAEALSNRELIEEKYRGIRPAPGYPA
                     CPDHTEKGVLWQLLQAEKAIGLKLTESYAMVPTAAVSGFYFSHPDAHYFSVGQVGRDQ
                     VESLAARKNLPLSEMERWLSPILGYDRSAAA"
     gene            complement(11444..>11631)
                     /locus_tag="VFY27_00910"
     CDS             complement(11444..>11631)
                     /locus_tag="VFY27_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764099.1"
                     /translation="IFFMANPALKALRDYEAQRKRGVEQYSFDPEALDIPHADYWVAR
                     LRSESSAAGARRTSNDR"
BASE COUNT         2248 a         3588 c         3651 g         2144 t
ORIGIN      
        1 acctggtcac ggaccttgct ggtggcatcc agcatcgcac gaagccgggc gttctccgac
       61 tcgagcgccg tgaagcgctg caagcgagcg tgcgtcagca gccgctcagc attcagccgg
      121 cctatttcca gcaacaaatc gttttgcgaa gccgtgttgt ccctgatcca ctgccacgtg
      181 gacaccggcg cgtccaccag gatccggagc gggtacaccg cggtgccgac ggccttacgc
      241 acggcgtcga ggtggttttc gcgatgatcg acgaccagca ggacgacgct gagcgctgcg
      301 agcagcaaca ggcgcaggcc aagcgcgggt gatttcgtcg accggtgctc gctgtcagcg
      361 gtacttgcga ccatcagttc agttgcgttg aaacgcgctg ccgctgccgc ggcgacacca
      421 ccggggcgag ggacggcagc ttcaggaaaa gcccgaattc actcctggtc gtcactcgag
      481 gccgaatacg gagggaccat gttcgtcgat cagttcaatt acccggccgc cgccgcgcgc
      541 cacgcacgtc agcggatcgt cggcaatgat caccgggagc ccggtttctt ccatcagcaa
      601 tttgtcgata tcccggagca tggcgccgcc accggtcagg acgatacccc gttcggcgac
      661 gtcggctccg agttccggcg gcgtttgctc gagagcttcc ttgaccgcgc cgacgatccc
      721 ttgcaacggc tcctgtaacg cttccagtat ctcgttgctg ttcagtgtaa aacttctggg
      781 cacgccttcg gacaggttgc gccccttcac cgaaatctct ctcacttcct gtccgggaaa
      841 tgctgacccg atttcgtgct tgatgcgttc ggcagttgcc tcgcctataa gaatgccata
      901 gttgcggcgg acgtagttcg tgatcgcttc gtcgaagcgg tcgccgccga ttctcacgga
      961 ggcggcgtag acgattccgt tgagcgagat gaccgcgact tcggaggtcc cgcccccgat
     1021 gtccagcacc atcgagccac gtgcttcgtg aacgggcatg cccgcgccga tggcggccgc
     1081 catcggttcc ggaatgagca ggaccttgcg ggcgccggca ccttcggcgg actcccggat
     1141 ggcccgtctc tccacctggg tcgaaccgta cgggacgcac acgagcactc gcgggctggg
     1201 gcggaagaag cgcttgccgt gagccttgcg aatgaagtgc tgcagcatct tttcagtgac
     1261 ggtgaagtcg gcgatgacac cgtctttcaa tgggcggatg gcagtgatgt tgcccggtgt
     1321 cctgccgagc atgttcttcg cttcggcacc gacggcttcg acaactctgc cgccgcgtcc
     1381 gctgtcttcg cgtatcgcaa ctaccgaagg ttcgtcgagt actatgccat gtccgcggga
     1441 gtagatcagt gtgtttgcgg tccccagatc gattgataag tcgttcgaga agtaccctag
     1501 aatgttcttt agcatgtggg gctatcgtcc cgcgaggcaa tttaactttg taatctaaca
     1561 atgcgcagga cattgagcaa gacggcgtgt atcattgctg aattttgccg ggtctggcat
     1621 cgctcgggct cttttgccgg actcgtcggg actctttcgt gtcgctgacc aaagaccagg
     1681 ttcaccacat cgcgttgctt gctcgactca agctcgagga gagcgagttc gacgatgtag
     1741 tcgacaagct gtcccggatc gtcgacttcg tcgaccagct ccaggccgcc ccgacagagg
     1801 gcgtcgtacc gatggctcat cccctggacc agtcgcagcg tttgcggccg gatgtcgtca
     1861 ccgaaacgat agaccgggac gctatccagg caaacgcccc cctggccaga gatggtttct
     1921 acctggtacc gaaagttctc taagtaatga gtgtggacgc cgggtccctg catgttctga
     1981 cgctcaccga acttgcaaga ggcataagag agggcgattt ctcggcactg gagctgacgg
     2041 aaacattgct ccggcgcatc gacgcacaca acttcgacct gaacgccttc atcacggtca
     2101 ccgccgaaca ggcactcgcc tcggccgagg ctgccgacaa ggctcgcgcg gcgggcaacg
     2161 cgccggtcct gaacggtctg ccgatcgtcc acaaggacct gttctgcacg agtggaatcc
     2221 ttaccacgtg cggctcccgg atgctgtcga atttcgtctc gccgtacgat gcgaccgtcg
     2281 tcgcgcggat gaaatctgcc ggtgccgtga cgctcggcaa aaccaatatg gacgagttcg
     2341 cgatgggctc gtccaacgag acgagctggt tcgggccggt ggcgaatccc tgggaccctg
     2401 ctcgctctcc gggcggctcg tccggaggct cggccgctgc agtcgccgcg agactggtgc
     2461 cggcgggtac cggcaccgat accggcggct cgatccgcca gcccgcggca ctgaccggca
     2521 cggtcgggat caagcctacc tacggtcgcg tctcgcgcta cggcatggtc gcgttcgcgt
     2581 cgagcctcga ccaggccggc gtcatcacgc gcagcgcgga ggacgctgcg cttttgctcg
     2641 gcgtcatggc cggcttcgac gaacgcgact cgacttctgt cgaccagcct gtccccgatt
     2701 accttgcggc attgggaaca tcgctcaagg gattgaagat aggcatcgtg cgccagcact
     2761 tcgacgaggg tctcgatccg cagtgcgccg cccggataag ggaagctttg gaggtactgc
     2821 gcgccgaagg tgcaacactt gtcgaagtcg acttgccgaa tctcgagctc tccgtgccga
     2881 cctactatgt cgtggcgccg gccgaatgct cgtccaacat gtcgcgcttc gacggcgtgc
     2941 gtttcggcca cagggcggcc gatcctgaag acctccttga cctgtattgc cgcagccgcg
     3001 gcgaaggctt cggcgcggag gtcaagcgcc ggatcatgac cggcacctat gtgctctccg
     3061 ccgggtacta cgacgcgtat tatttgaagg cgcagaaagt gcggcagctg ataggcgacg
     3121 acttcaggaa agcctttggt caagtcgatg tgatcgccgg gccgacggcg ccgacgcctg
     3181 cgttcgcact cggagagaaa accgacgacc cgatcacgat gtacctgaat gacatttata
     3241 cgatcggcgc gaacctcgcc ggcaaccccg ggatttcgct gccctgcgga tttgcggaaa
     3301 agcttcccgt cggattacag ctcgtggggc cgcacttctc ggaagaaaaa ctcctgaacg
     3361 tcgcgcacca gtaccagctg ctgacagact ggcacaaggc ctgtcccgag gcctttcaat
     3421 gagcgtgccg gccgcccatt cacgtgccgc ttctgaatgg gaagcggtta tcggtctcga
     3481 gattcacatg cagatcgcga cggcgagcaa gatattctcc gccgcgccga cgacctacgg
     3541 cgcagaaccc aatacccagg cttcgctgat cgatctgggt tatccgggtg tcctgccggt
     3601 gctgaacgag gacgtcgtgc gcatggcctg caagttcggg ctagcggtga acgcgagtat
     3661 cgcgccgcgc tcggtcttcg cgcgcaagaa ttacttttat ccggacttgc cgaagggtta
     3721 ccagattagc cagtatgagc tgccgatcgt ggcgcgcggc gagctgacga tcctcgacga
     3781 gcagggcagg gagaaacgca tcggcatcac ccgggcccac ctcgaggaag acgccggcaa
     3841 gtcgctgcat tcgggcttcg acgaattcac gagaatcgac ctgaaccgcg ccggtacgcc
     3901 actgctcgaa atcgtctccg atcctgattt gcgatcggcg aaagaagctg tcgcctacat
     3961 gcgaaaggtg cacacgctcg tgcgctatct cgagatatca gacggcaaca tgcaggaagg
     4021 ctctttccgc tgcgacgcca acgtttcgat tcggcgttgc ggcgaccaga ctctcggtac
     4081 ccgcaccgaa ctgaagaacc tgaactcgtt tcgcttcgtc gaaagggcca tcaacttcga
     4141 gatcgaacgg cagcaggagg ttctggaaga gggcggcacc gtcctgcagg agacgcgtct
     4201 ttacgattcc gaactggacg agacgcggcc catgcgaacc aaggaagaag caaacgatta
     4261 tcgttatttt cccgaccctg acttgctgcc ggtcgtcatt gaagaggcgt ttatcgaatc
     4321 cgtccgcgag acgctgccgg aactccccga cgcgaaacgc cagcgcttcg tcgagcagta
     4381 cgacataaga gcaggcgatg cggaaattct caccacgagc cgcgggctgg ccaactactt
     4441 cgaagcgaca gctgcgagtt cggaccctaa gctcgccgcg aactggatcc tcggagacct
     4501 gtcggcagcc ctgaaccggg agggactcga gatcgacgac agccgcatcc ctcccgaagg
     4561 actggcggag cttgttgcgc gcattgcaga cagcacgatc tccggcaaaa tcgccaagga
     4621 ggtgttcgat gcgatgtggg ccggcgaggg cacggcgtcc gggattatcg atgtgcgtgg
     4681 cctgcggcag ataacggacc cttccgccat cgaggccgta gtcggcaaag tgatcgaggc
     4741 gaatcccggc caggcagcgg actatcgttc cggcaaggat aaactcctcg gcttttttgt
     4801 cggacaggtc atgaaagaga caagcggcaa ggcgaacccc gcggaggtca acaggatcct
     4861 gaaggagcga ctgaagtcct gatggctgcg cacgacgtcg tcatgccatt cgtcttcgag
     4921 gcacttcccg tccgcggcgc cataatccag ctgcagaagt catggcaccg catgctgctc
     4981 ggccacaact acgaccagcc ggtgctcgag acgctcggcc actccgccgc ggccagcgca
     5041 ctgatcgcgc agagcctgaa gttcgacggc tccatcaccc tgcagatcaa cggcaacggc
     5101 ccgctcgcca tgctggtcat gcagtgcacc agcaacctcg aactgcgcgg tatggccagc
     5161 gccgggcaac tcgacgacga catcgccttc agcgaactgg tgcggagagc tcgctgcgcc
     5221 atcaccgtaa atgctggcgc gatggagcgt ccctaccagg gcatcatcga agtcagcggt
     5281 gactccctgg ccgacagcct ggaggattac taccggcgct ccgcacagat cccgacccac
     5341 ctccggctcg tcagcgaccg caactgctgc ggcggaattc tcctgcagca gatgccgggt
     5401 gaacaaagcc ccgtcgccga cgactggcgc cgcgtcgggc tcctggccgg caccctgcgc
     5461 attcacgacc ttgcggacgg cctggctcct tctctcttag gtcgcctctt cgccgaagac
     5521 gacctgcgcg tgtttcgtcc ccgcgaagcc cagttccgct gccgctgctc gcggcgccgt
     5581 tccgaagacg tgctcaaact tctgggcgag caggacaccc gcgatctcct ggccgagcag
     5641 ggcagaatcg aagtcacctg cgaatattgc ggacgcacac gcacgtttga ttcgatcgac
     5701 atctcccaac tctttaccga acctccggta gcgacctccg atacgttgca ctagggtgct
     5761 ggggctggac cgccgtgtcc ggaggtggtc gctccatggc tcgacttcct tgcgccgcga
     5821 accccgtgat acactccgcg gcaacgcata aagatatcgt tatacgttaa tgaaacctgg
     5881 aaacgagcgc cttctcctgc agttcaagat cctggccgat cccttacggc tgcggctggt
     5941 ggcgctttgc cggcgggggg agtgcagcgt gtcggaactg acgcaggcgg tggggcaaag
     6001 ccagccgcgg gtttcgcagc acctgaagaa gcttgttgag gcgggattgc tggagcgatt
     6061 ccgggacggg aagcgcgtgt attacagggt gccggcggcg gaagggcatg cggcgcggcg
     6121 gttgctggat atgttgccgg atgcggaacc gttgatgctg gaggatgccg ggcgcctggc
     6181 ggccatgcgg gcgcagaggg ggtttacgca tcccgtgagt gacgccggtg tcggcactgc
     6241 caaccgggcg atgcatcggc tgctgctgga gctgaccgtt accgcgcctg tcggcaactt
     6301 gctggacatc ggttgcggtc agggacgtgt gctcaagctg ctggcgagtc gtgcgaatcg
     6361 cgcgatcggc gtcgacattg atcctcaggc gcggctgacc gcacgcacgg acctcatgct
     6421 ggcgggattg ccgaattgca gcctccggca cggtgacatg taccggctgc cgtttgctga
     6481 cggtgagttc gacaccatca ttctcgacga cgtcctcgcg gacgcggtcg atcccgtgga
     6541 tgcgttgcag gaggcgaagc gcctgctgca tccgaacggg cgcctgctcg tgttacaggc
     6601 gttgcgcggc cagcccgtaa tggcattgca gaagtcgctt gccgagtgga gccgcgccgc
     6661 ggccttgcgt ctcgctccgg cgcgcttcgc gccggaaaaa acgccggaat ggcttctgtg
     6721 cgtggcaacc cgcgcagacg caaacgcggc ggctgcctga ctttcatcga aagaggatcc
     6781 ggatgaacga ttcgcgcacg gctacgcagg tttctttcga gttctttccg ccgaagacgg
     6841 aggaaatgga gaagaccctg tgggagtcca tcgagaggct cagctcactg tctccgcgct
     6901 tcgtttcggt gacgtacggg gctgacggct ccactcgtga gcgaacacac gcggcggtag
     6961 cgcggctgct gaaggacacc tccctcactg ccgcgccgca ccttacctgc gtcggcgcaa
     7021 gcaggggcga gatcgacgac attgcgcgcg gctattggga catgggcgtg cggcatcttg
     7081 tcgcgctgcg cggcgacccg ccgaaagcgg cggacgtccc ctggcaggca tatgcgccgc
     7141 atccggatgg ctatgcctat gccagcgatc tcgtggccgg cctcaaaaag gtggcggact
     7201 tcgacatttc cgttgctgcg tatccggagg tccacccgga agcgcaggat gcgggtacgg
     7261 acctcgaaaa cctcaagcgc aaaatcgacg ctggcgcctc ccgcgctatc acgcagttct
     7321 ttttcgatgt cgacacctac ctgcgttttc gcgaccgctg cgcagccagg agaatcgaga
     7381 cggcgatcgt tcccggcatc ctgcccatta cccgtttccc gcaattgcag cgtttcgcgg
     7441 agcagtgcgg ggcgtcagtg ccggagtggc tgcacgagcg gttccagggc ctggaggacg
     7501 atccggaaac gcgccgcctc atcgccgcga gtgtcgcgat cgagcaggtc cggcagttga
     7561 aaaaagaagg catcaacgaa tttcattttt acaccctgaa ccgatccgaa ctgacgtatg
     7621 cgatctgtca tgcgctgggc gtgagggcag agcaggcggc tgcttaagtg cactaaggaa
     7681 aagccatgca acgcgagcag cgaatagaac tactgaagaa actcctggcc gaacgcatcc
     7741 ttttgctcga cggagcgatg ggcacgatga tccaggctta tcggctttcc gaggacgatt
     7801 atcgtggcga gcgctaccgc gagtggcccc gtgacctgaa aggcaacaac gaccttcttt
     7861 caattacgcg accggaagtg attcgtgata ttcaccgcca gttcctcgag gccggtgccg
     7921 atatcatcga gaccaacacg ttcaattcca atgcgccgtc gatgggcgac tacggcatgg
     7981 agacgctggt ctcggacctg aaccgcgccg ccgcaagtct cgcgcgcgaa accgccgaca
     8041 gcttcgcgga gcggagtggc gagccgaaat tcgtggcagg tgtgctgggg ccgagcaatc
     8101 gtactgcatc gatttcgccg gacgtgaacg atcccggctt ccgcaacatc accttcgagc
     8161 ggctcgcgga aacataccag gtggctgcga ctgcgcttat cgaaggcggc gtcgacttcc
     8221 tgatgatcga gacgattttc gacacgctca atgccaaggc ggcgatcttt ggcctgcgcc
     8281 ggagcttcga agcggccggg ttcgagctgc cggtcatgat ttccggcacg atcaccgacg
     8341 cgtccggccg cacgttgtcc ggacaaacga ccgaggcgtt ctggaattcg gtgcgccacg
     8401 cgaagccgtt catggtcggc ctgaactgcg cgctcggtgc ggccgatctc aggccctacg
     8461 tcgcggagct ttcccgcatc gccgacaccg gcgtcagcgc ccacccgaac gcgggtttgc
     8521 cgaacgagtt cggcgagtac gacgagacgc ccgaagagat ggcgggaatt atcgtcgagt
     8581 tcgcgtcgag cggcctggta aaccttgtcg gcggctgctg cggcacgaca cccgatcaca
     8641 ttcgccgcct gcgggaggcc attgccggca aggcgccgcg aaacataccg tcggtgccgg
     8701 tccgctgccg cctgtccgga ctcgagccgc tgaacctggg gccggacgac ctgttcgtca
     8761 acgtcggcga gcgaagcaat gtcacggggt cgaagctttt ccgccgcctg atcgaagccg
     8821 gcgaatacgg ggaagccgtg cagatcgcgc gccagcaggt cgaaaatggc gcgcaaatca
     8881 tcgacgtcaa catggacgag ggcatgctcg actccgagaa ggccatgcag acgtacatca
     8941 acctgatttc gtcggaaccg gacgttgcac gcgtgccggt aatgatcgac tcgtcgaagt
     9001 ggacggtgat cgagacagga ttgcgctgcg tacagggcaa gtcgatcgtc aattcgatca
     9061 gcctcaagga aggcgaggag gcattcatcc ggcaggcgcg cctcgtgcgg gattacggcg
     9121 cggcagtcgt ggtgatggcg tttgacgagc agggccaggc ggacagtgtc gcgcgcaagg
     9181 tcgcgatctg caaacgctgc catgacattc tgacgaagca ggtcgggttc gacccggccg
     9241 acatcatttt cgatccgaac atttttgcgg tcgcaaccgg catcgaagag cataaccgct
     9301 acgggctgga ctttgtggag gctacgcgtg cgatcaagga agcgctaccg cacgtaatgg
     9361 tgagcggcgg cgtcagcaac gtctcgttct ccttccgcgg caacgacgtc gttcgcgagg
     9421 ccatccattc cgtattcctg taccacgcca tcaaggccgg catggatctg ggaatagtca
     9481 acgcgggcca gctcgcgatc tacgaggacc tgccgaagga cctcagggat gctgtcgaag
     9541 acgtgatcct tgcccggcgt gacgatgcca ccgagcgcct gctggaagtc gccgaatcct
     9601 acaagggact cgcaggcgga cagaaggcga aggagaaaga cctcggttgg cgcgacctcc
     9661 cggtgaacaa gcggctcgag catgcgctgg tcaacggaat tgacgagtac gtcatcgagg
     9721 acacggaagc agcgcggctc gccgcccaaa aaccactcga cgtgatcgag ggaccgctga
     9781 tggatggcat gaatgtcgtc ggcgatctct tcggcgccgg caagatgttc ctgccgcagg
     9841 tggtcaagtc ggccagggtc atgaagaaag cggtcaacca tctcgtgccg ttcatcgaga
     9901 aggagaaggg cggcgcgagg aaaagcaacg ggcgcatcgt catggcaacc gtaaaagggg
     9961 atgttcacga catcggcaaa aacatcgtcg gcgtggtcct gcagtgcaac aacttcgaag
    10021 tgatcgacct cggcgtcatg gttccctgcg aaaagattct cgagaccgcg aagcgcgaga
    10081 acgcgcagat catcgggctg tccggcctga ttacgccgtc gctcgatgaa atggtgcacg
    10141 tggcaagcga gatgcagcgg ctgaagtttc agttgccgtt gctgatcggg ggcgcaacca
    10201 cttcaccggc gcacacggcg gtgaaaatcg agccaaacta cgagggcccg gtgatctatg
    10261 tgaaggacgc ctcccgctcg gtcggcgttg cccaggcgct cgtcgaaact gcatcgcgcg
    10321 acgaactcat aaccaggacg cgacgcgaga acgctacgcg gcgcgaacgt catgcaggaa
    10381 agacgcggct ggcaccgcag ctgtccctgt cacaggcacg cgaaaggaag catcgctgcg
    10441 actgggtgaa ttatgtcccg ccgcggccat cgttctccgg cacccgtgtg ctcgacaagg
    10501 tggacctggc gacgttgcgc gaccacatcg actggatgcc gttcttcaac gcgtgggaat
    10561 tccacgggaa gttcccggcc attctcgatg acccgtcggt cgggagttcg gtgcggccgc
    10621 tttatgcgga cgccaacgcg atgctggacc ggatcatcgc ggagggctgg ctcgaggcgc
    10681 gggccgtctt cggcttcttc ccggcgagta ccgtcgacga cgacgatatc ctggtgtacg
    10741 agaacgactc gcggcagaag tcgctattgc ggctttgtca catgcggcag cagcggggga
    10801 agccttcggg gatggcgcag gattgcctgg ccgatttcgt cgcacctgct ggtagcggca
    10861 tcgacgatta catcggcgcg ttcgcggtaa cggcgggcat cggcatagac gagcacgtgc
    10921 agcgtttcga agcagatcac gacgattaca gcagcattat tctcaaagcg cttgccgacc
    10981 ggctcgccga agcactcgcc gagtacctgc acctcagggt gcgcagggag ttctggggtt
    11041 acgaccccgc cgaggctctg tccaatcgcg aactcatcga ggagaagtac cgcggcatac
    11101 gcccggcgcc cggctatccc gcttgcccgg accatacgga gaaaggcgtt ctttggcagc
    11161 tgctgcaagc ggagaaggca atcgggctca agcttacgga gtcgtatgcg atggtgccta
    11221 ccgcggcggt cagcggcttc tacttctcgc acccggatgc gcattatttt tcggtcggac
    11281 aggtcggtcg cgaccaggtc gaatcgctgg cggcccgcaa gaacctgccg ctcagcgaaa
    11341 tggagcgctg gctctccccg atcctcggct acgaccgctc cgccgccgcc tgatccacct
    11401 gcaggagccc gccccgccgg cgacaatacc gttcgaaact gcgctaccga tcgttgctcg
    11461 tacgacgggc tcctgcagcg ctcgattccg aacgaaggcg tgctacccag tagtcggcgt
    11521 gcggaatgtc cagagcttcc gggtcgaacg aatactgctc gacaccccgt ttgcgttgag
    11581 cttcgtaatc ccgtagcgct ttcagcgccg gattcgccat gaagaaaata a
//
LOCUS       DASUOM010000043         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1431791,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000043 DASUOM010000000
VERSION     DASUOM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431791"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFY27_00915"
     CDS             complement(<1..1383)
                     /locus_tag="VFY27_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764100.1"
                     /translation="MFLSSVLMLACTAPRMIGYSRSSVVCRDLREAPHSRWETTVDGG
                     VHWLLTPCGERFFSLGVNVMDGGEPQRFSNGRIAYHWGTFYADLEHWAQAARQRVLGW
                     GFNTAGGWSLHPTVLGLPITPNLELGRTARFHWFDPFDPATAERMYSLARRLVSPYKG
                     NPYRLGYFIDNEVGWWNGALFLHYLKQPATNYTKQHLIARLREYYGNDWGRFTQDFVP
                     PQDAGSFEHLLHHTHATQLRPGGAGIQFVRHWTGIITSLYYRLAHDAIRAADPDALIL
                     GDRLPIYYDPVALRTMVQHVDVISTNYNVDSPDGWLARYFFDGLRQLAPRKPILISEW
                     FFAADENRSGNLNNSHLMTVPTQAERTRGAIAAAQRFARQPQIVGLQWFQYYDHPYGG
                     REDGEDYNFGLVDINDRPYERLVEAFSRVNPDLPAVHEQMSDTTLPVRDGPWRIPHAA
                     IDVRDRSLGEW"
     gene            complement(1416..2069)
                     /locus_tag="VFY27_00920"
     CDS             complement(1416..2069)
                     /locus_tag="VFY27_00920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5764101.1"
                     /translation="MIQRELVDQKMRSFMDSLWERGDPWDIENSTYERDRCVQLLNML
                     EGRRYARVLEIGCGAGYLTRLLAPHADHIVALDISETAIDRALALGTGHTKVDFRVAN
                     IMDYKPELDGPWDLVVFSDTMCYLGWLYPLFDVAWLAVQIFEATRTGGRLMLANSMYE
                     GEDWLLRPWIIRTYRDLFVNVGYRIESEEVYKATKRGVEFEVLMTLYCKPEEGKQGK"
     gene            complement(2091..2951)
                     /locus_tag="VFY27_00925"
     CDS             complement(2091..2951)
                     /locus_tag="VFY27_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5764102.1"
                     /translation="MEKLLPEHLKHCWESVERRERSREDYTREYERAIGEYRQTWERA
                     LTLPEHDDLRESLLAELGSYTQCSDLAEVRRRCEQAVANLKREWEHKVQSVDRQSIEQ
                     FYDESPAMLHELMWWHALGDDLSPLAYVLALRFAEQHGCNSYLDFGAGVGAGGLLFAA
                     HGLQTNLADISSSLLRFSAWRLGLRKLPAQYLDLKRCHLPSECFDFITAMDVFEHLSD
                     PVETVEKLWNALKPGGFLYGRISAEADEDRPQHIVHDFEPTFARMRALGFVEVWRDEW
                     LWGHQVFQKL"
     gene            complement(2951..>3622)
                     /locus_tag="VFY27_00930"
     CDS             complement(2951..>3622)
                     /locus_tag="VFY27_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX5764103.1"
                     /translation="VRWPAAAEAAAYARKHPELSVGLHFDLGEQILRAGEWVPLYTVV
                     PLQDESAVQDEISRQLGAFDRLMEHAPTHLDSHQHVHLREPVRAILLDIAQRLGVPLR
                     SCSPEVSYCGSFYGQADDGTTLRDVISVDGLIRILKTLPAGFTELACHPAAECDVQTL
                     YSQERLEELKVLCDPRVRAALTDNGIALRSFANLSQGDKRSVAEVHTELNFGKHSIRP
                     RKQEE"
BASE COUNT          812 a         1056 c         1027 g          727 t
ORIGIN      
        1 ccattctcct agtgagcgat cgcgcacatc gattgctgca tggggaatcc gccatgggcc
       61 atcgcgaacc ggcagggtgg tatcggacat ctgctcatgg acggccggaa gatcaggatt
      121 gacgcgactg aacgcctcga caagccgctc atagggtcgg tcgttgatat cgacgagccc
      181 aaagttataa tcttcgccgt cctcacgccc tccgtacggg tggtcataat actgaaacca
      241 ctggagaccg acgatctgtg gctggcgagc gaatcgctgg gcggcggcta tggcgccgcg
      301 agtgcgctca gcctgcgttg gcacggtcat caagtggcta ttattaaggt tgccggagcg
      361 attttcgtct gcggcaaaaa accactcaga aattagaatc ggcttgcgcg gggcgagctg
      421 tcgcaggcca tcaaaaaaat accgggcgag ccatccatca gggctatcga cgttgtagtt
      481 ggtggatatg acgtcgacat gctgcaccat tgtccgcaac gcgaccggat cgtaatagat
      541 aggcaggcga tcaccgagta tcagagcgtc aggatcggcc gcacgaatgg cgtcatgagc
      601 tagccgatag tagaggctcg tgatgatccc cgtccagtgg cgtacaaatt ggattcccgc
      661 accaccaggt cgcaactgcg tggcatgagt gtggtgaagc aaatgctcaa aagagccggc
      721 gtcctgaggc ggcacgaaat cctgtgtgaa gcggccccag tcattgccgt aatattcacg
      781 cagtcgagcg ataagatgtt gcttggtgta attggtggcg ggctgcttta gatagtgtaa
      841 aaatagagca ccattccacc agccgacttc attatcgata aagtagccga ggcggtacgg
      901 attgcctttg tagggggaga ccaggcgccg tgccaaggaa tacatgcgct cggcggtggc
      961 gggatcaaat gggtcaaacc aatgaaagcg ggcggttcgc cccaattcga gattgggagt
     1021 gatcggcagc cctaacaccg tgggatgcaa ggaccagcca ccggcagtgt tgaagcccca
     1081 ccctaatact ctttgccgcg ccgcctgcgc ccaatgttcc aagtcagcat aaaaggttcc
     1141 ccaatggtaa gcgatacgcc cgttggaaaa tcgctgcggc tcgccgccgt ccatgacatt
     1201 gacaccgagc gaaaagaagc gctcgccaca tggtgtaagt agccaatgca cgccaccgtc
     1261 gacggtcgtc tcccagcgcg aatgaggcgc ctcccgcagg tccctgcaaa ccaccgacga
     1321 gcggctgtaa ccgatcatcc gaggggctgt gcacgccagc atcagaacgc tggaaaggaa
     1381 cacgcaaatg ctcggcgcta gccaccgacg aactatcact tgccctgctt tccctcctcg
     1441 ggcttgcaat agagcgtcat gagtacttcg aactcaacac cgcgtttcgt cgccttgtag
     1501 acttcctcac tttcgatcct gtagcccaca ttcacaaaga gatctcggta cgtgcggatg
     1561 atccagggac ggagaagcca atcctcgccc tcatacatgc tattggccag cattagtcgc
     1621 ccgccggtgc gtgtcgcctc gaaaatctgt acggcaagcc aggcgacgtc aaacaatgga
     1681 tagagccagc cgagataaca catcgtgtca ctaaaaacga cgagatccca cggcccgtca
     1741 agctctggct tgtagtccat aatattcgct acgcggaagt ccaccttcgt gtggcctgta
     1801 ccgagtgcaa gcgcccggtc aatggctgtc tccgaaatgt cgagcgcaac aatgtgatcg
     1861 gcgtgagggg cgaggagccg agtgagataa ccggcgccgc atccgatctc caacacgcga
     1921 gcgtagcgtc tgccctcgag catgttgagc agctgtacac atcgatcccg ctcataagtc
     1981 gaattttcga tatcccatgg gtcgccgcgt tcccacaggc tgtccatgaa actccgcatt
     2041 ttttggtcga cgagttcccg ctggatcata aaattctccc cttctcttcc tcacagtttt
     2101 tgaaagactt ggtgacccca aagccactca tcacgccaga cctcaacgaa tccgagtgcg
     2161 cgcatacgcg cgaacgtcgg ttcaaaatcg tgcacgatgt gctgaggccg atcctcgtca
     2221 gcttctgccg agatccgccc gtagagaaac ccgcctggct tgagcgcatt ccacagcttc
     2281 tcgacagttt ccacggggtc actcagatgt tcgaagacat ccatagctgt gatgaaatcg
     2341 aagcactcgc ttgggaggtg acaacgctta aggtcgaggt attgggcagg caattttctg
     2401 agccctaatc gccaggcgct gaagcgcagc aaagaggagc taatgtccgc caggttggtt
     2461 tgtagcccgt gagccgcgaa gaggagcccg ccggctccca cgccggcacc gaagtcgagg
     2521 tagctgttgc agccgtgctg ctcggcaaag cgcagcgcca ggacataggc gagcggcgag
     2581 agatcatcgc caagggcgtg ccaccacatg agctcgtgca acatggccgg actctcatcg
     2641 taaaactgtt cgatggactg ccggtccacc gattggacct tgtgctccca ctcacgcttc
     2701 aggttcgcga cggcctgctc gcagcggcgc cggacctcag ccaggtcgga gcattgcgtg
     2761 tagctgccga gctcagccaa aagactctcg cgtaaatcgt cgtgttcagg caaggttaga
     2821 gcacgctccc aagtctgccg gtattcaccg atggcgcgct cgtattcgcg agtgtagtct
     2881 tcacgggaac gttcacggcg ctccactgac tcccaacagt gtttcaagtg ctcgggcagt
     2941 aacttctcca tcactcctcc tgcttacgcg ggcgtatcga atgtttaccg aaattcaact
     3001 cggtgtgcac ctctgcgact gaccgtttgt cgccttgcga aagattggca aacgaacgga
     3061 gcgcgatgcc gttgtccgtt agcgcggctc gtacccgggg atcacacaaa accttgagct
     3121 cctccagacg ttcctgagaa tataacgttt gcacgtcgca ctccgccgct ggatggcatg
     3181 ccagctctgt gaaacccgcc ggcaacgttt tcaaaatacg aatcaggccg tcgacgctaa
     3241 tcacgtcacg caaggtcgta ccgtcgtctg cttgcccata gaagctgccg caatagctga
     3301 cttccggaga gcaacttcgg agcggaactc cgaggcgctg ggcgatgtcc aagaggatgg
     3361 cgcggacagg ttcgcggaga tgaacgtgct ggtgcgagtc gaggtgagtc ggagcatgtt
     3421 ccatcaggcg atcgaatgcg ccgagttggc gggagatttc atcttgcacc gcactctcat
     3481 cctgcaatgg gactacggtg tagaggggca cccattctcc ggctcgcaag atctgttcgc
     3541 cgaggtcaaa gtgcaaccca acactcagtt ctggatgctt tcgggcataa gcggccgcct
     3601 cagcggcagc cggccagcgc ac
//
LOCUS       DASUOM010000044         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_220243,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000044 DASUOM010000000
VERSION     DASUOM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220243"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFY27_00935"
     CDS             <1..900
                     /locus_tag="VFY27_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5764104.1"
                     /translation="PMPELPDVELYLHALGSRIVGRTIERVRLASPFLVRSFDPPVSA
                     IEGRSVAGLHRLGKRIVWELDDELFVVIHLMIAGRFRWKPPGAAVPGKIGLAAFDFAN
                     GTVLLTEASPKKRASLHVVRGRAALKAHDPGGLEVLAATGAQFAERLRLENHTVKRAL
                     TDPRLFSGIGNAYSDEILHAARLSPLQWTSRLSDDDVARLFDATIRVLRTWTERLARE
                     TGDLFPEKVTAFREDMAVHGRYGRPCPECGSTVQRIRYADNECNYCATCQTRGKLLAD
                     RSLSRLLGADWPKTLEELERLKA"
     gene            complement(939..1643)
                     /locus_tag="VFY27_00940"
     CDS             complement(939..1643)
                     /locus_tag="VFY27_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5764105.1"
                     /translation="MDAPDLALGLLWFAAFLFSTTVHEAMHAWAAWKGGDPTAYHGGQ
                     VSLSPVPHIRREPIGMVVLPLLTALTQGWAIGWASAPYDPRWAARYPKRAAVMAAAGP
                     VGNLLIASAAFIVLKIGLNQDLFMAPPRVSIDSLVMPAGGNAGDFISTLLSILLMLNV
                     FLCVFNLIPLPPLDGSAVLALILPERQALQMQQYAAQPWLQLVGLAIAWKVFPIISGP
                     LFLIVLRLLHPAEAYG"
     gene            complement(1768..1884)
                     /gene="rrf"
                     /locus_tag="VFY27_00945"
     rRNA            complement(1768..1884)
                     /gene="rrf"
                     /locus_tag="VFY27_00945"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2016..>2438)
                     /locus_tag="VFY27_00950"
     rRNA            complement(2016..>2438)
                     /locus_tag="VFY27_00950"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          466 a          807 c          750 g          415 t
ORIGIN      
        1 cccatgcctg agctcccgga cgtcgagctg tacttgcacg ccctcggctc gcgcatcgtg
       61 ggccggacga tcgagcgcgt gcgcctggcg agcccattcc tggtccgctc gttcgacccg
      121 cccgtttccg cgatcgaggg ccggtcagtc gccggtttgc accgcctggg caagcgcatc
      181 gtctgggaac tggacgatga gctgttcgtc gtcattcacc tgatgatcgc cggccgcttc
      241 cggtggaagc cgccaggagc cgccgttccc ggcaagatcg ggctcgccgc gttcgacttc
      301 gcgaacggga cggtgctgct gaccgaggca agcccgaaga aacgggcatc gctccatgtc
      361 gtgcgcggtc gagcggcgtt gaaggcacac gaccccggcg gcctggaggt gctcgcggca
      421 accggggcgc agtttgccga acgattgcgg ctcgagaacc acacggtcaa gcgtgcgctc
      481 accgatcccc gcctcttcag cggaattggc aacgcctact cggacgagat cctccacgcc
      541 gcccgactgt cgccgctgca gtggacctcg cgtctttccg acgacgacgt cgcacggctg
      601 ttcgacgcga ccattcgggt acttcgcaca tggaccgagc ggctggcgcg cgagacaggc
      661 gatctgtttc ccgagaaggt cacggcgttt cgcgaagaca tggccgtcca cggccgatat
      721 ggcaggccct gtcccgagtg cggatcgacg gtgcaacgca tccgatacgc agacaacgaa
      781 tgcaactact gcgcgacctg ccagacccga ggcaagctgc tcgcggatcg cagcctctcg
      841 cggctgctcg gcgccgattg gccgaagacg ctggaggaac tcgaaaggct caaggcttga
      901 gtggaacctg ggctcagggc ttcgggctcc ggggtcgcct acccatacgc ttccgccgga
      961 tgcagcaggc ggagcacgat gagaaacagc ggtccgctga tgatcgggaa gaccttccag
     1021 gcgatggcga gccccaccag ttgcagccac ggttgagcgg cgtactgctg catctgcagc
     1081 gcctgccgtt cgggcagtat cagcgcgaga accgccgaac cgtcgagggg cggcagcggg
     1141 atgaggttga acacgcagag gaacacgttc agcatcagca gaatcgagag cagcgtgctg
     1201 atgaagtcgc cggcattccc accggccggc atcacgagcg agtcgatcga gacgcgcggc
     1261 ggggccatga acagatcctg attcagtccg atcttcagga cgatgaacgc cgccgacgcg
     1321 atgagcaggt tcccgacggg gccggccgca gccatgaccg cggcccgctt cgggtaacgc
     1381 gccgcccacc gcggatcgta cggcgcgctc gcccacccga tcgcccaccc ctgggtaagc
     1441 gcggtcagca gcggcagcac caccatgccg atcggctcgc gcctgatatg cggcaccggc
     1501 gagagcgaca cctggccgcc gtgataggcc gtcgggtcgc ctcccttcca tgcagcccac
     1561 gcgtgcattg cctcgtggac ggtggtcgaa aagaggaagg cggcaaacca gagcaggccg
     1621 agggcgagat cgggcgcgtc catgggcccc aatttagcag tcggatgccg ttggcgccga
     1681 aggtttctga cgtccgaacg gcctacgctg agaaccgtac aaagaaaaag cccgacactc
     1741 tttcgagcat cgggcttctg gtaaaactcc cggcagcgac ctactctccc acacccctgc
     1801 ggatgcagta ccatcggcgg tggcaggctt aacttccgtg ttcggaatgg gaacgggtgt
     1861 gaccctgccc ctgtgaccac cgggaacttg ctgactgtgg ggcggggctt cagccccgcg
     1921 atccgcggcc ctgagggccc cgcaccacat tgaataccct gcaacgatag ttttccgacc
     1981 acacacccac agcgtgaatt cgttcttatg ggtaaatggt caagcctcac ggctgattag
     2041 taccagtcag ctaaagacat cactgccctt acacacctgg cctatcacct ggtagtcttc
     2101 caggagcctt tagaggcctt acggcctggg agatctcatc tcgtggaggg tttcacgctt
     2161 agatgctttc agcgtttctc ccttccgcac ttagctactg agcgctaccg ctggcgcgat
     2221 aactcataca ctagaggtgc gttcaacccg gtcctctcgt actaaggtca gccccactca
     2281 aatctcctgc gcccatcaca gatagagacc gaactgtctc gcgacgttct aaacccagct
     2341 cacgtaccgc tttaaccggc gaacagccgg acccttggga gctgctacac ccccaggatg
     2401 cgatgagccg acatcgaggt gccaaacctg agcgtcga
//
LOCUS       DASUOM010000045         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1552118,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000045 DASUOM010000000
VERSION     DASUOM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552118"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFY27_00955"
     CDS             complement(<1..1076)
                     /locus_tag="VFY27_00955"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX5764106.1"
                     /translation="MKPVEPEFTIGVEEEYLLVDRGTRALIIDPPQSLIGECEERCGG
                     QVTSELLRSQVEVGTKVCRTVQEVRADLARLRSGIIEVAGRHNLAPIAASTHPFSRWS
                     EQKQTAKDRYDSLTAEMQGAARRLVICGMHVHVGISDEELRIDLMNQMSYFLPHLLAF
                     SCSSPFWEGRDTGLKSYRLTIFDALPRTGIPERFQSYAEYQRHVGVLINAGLIEDSTK
                     IWWDLRPSGRFPTIEVRVMDVCTRLDDCVAIVALLISIVRMLYRLRLANQRWRLYTTM
                     LIRENRWRAMRYSFDEGLIDLAKGAVVPVGELLDELIHLTAEDSEALGCTEEVASLRD
                     IMKRGTSAHRQLRTFSEAKEAGAS"
     gene            complement(1073..1930)
                     /locus_tag="VFY27_00960"
     CDS             complement(1073..1930)
                     /locus_tag="VFY27_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5764107.1"
                     /translation="MTYLPFTMPPRGTAGHPAVTDLLAADEPAPFSILNPSAGKPLLL
                     VCDHASRVFPRALGDLGLDPLARRCHLALDIGAGALTAKLSESLGATAVLAGYSRLVV
                     DCNRDLLDPGAFLEFGDGIVIPGNRNLQPEQKAARAEAIYWPYHRAIDAEIKRLAGEG
                     RSPAVIAIHSFTPVLDGTSRPWEFGILWDADPRIADILMRKLADSGFVVGDNEPYSGR
                     APQDFTVDNHAEAAGLPHAGIEVRQDLISNDAGVARVAGILHGIIDDLPTSIFGLPAA
                     GNYRKAQRQ"
     gene            complement(2152..>3986)
                     /locus_tag="VFY27_00965"
     CDS             complement(2152..>3986)
                     /locus_tag="VFY27_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5764108.1"
                     /translation="GREIVVLENSRAIYDDDGKVVAHEGTITDITERKIAETRVFEEK
                     ERAQVTLQSIGDGVITTDAEGRVDYINPVAQELAGLEIRNARGKPIDKIMTIVNEHTR
                     ATVENPVLRCLKEGRVIALADHSVLINQQGVEIAIQDSAAPIRDRIGNIIGAVMVFHD
                     VSKETRLFRQLSYQASHDALTGLINRREFENRLVAALDKAYGQGASHALLYFDLDQFK
                     VVNDTFGHTAGDELLRQISELVQSNIRSTDVLARLGGDEFGILLERCTEERAIEVSEA
                     IRSAVEAFRFTWQEAYTSVRCSIGIVMVNKDTQSVASIMSSADVACYSAKDMGRNQVH
                     LYQDRDASVRHEEMKWVSRITSAVEDNRFELFYQPIIGIGEKGAGARGHYELLLRMRG
                     EDGQIVGPDQFIPAAERYNLMSMLDRWVIREALSQLADRSHTSEQARFTLAINLSGTS
                     LSEDRFLEFVIEELTKQRLPQGAICFEITETAAISNLSRVIHFMQTLRKLGCKFSLDD
                     FGSGLSSFTYLKNLPVDYLKIDGQFIRNVAEDSVDESMVRAINEVGRAMGIATIAERV
                     ESKKVLDKLGDLGIEFAQGFFIARPTSVQTFEPWASDATASRLA"
BASE COUNT          690 a         1255 c         1264 g          777 t
ORIGIN      
        1 tggcttgccc cggcctcctt cgcctcgctg aaggtcctga gctgccggtg agcgctggtg
       61 ccccgcttca tgatgtccct cagggaggcg acctcttcag tgcagcccag cgcctccgag
      121 tcttcggccg tcaggtgtat cagttcgtcc agcagttccc ctaccggcac cacggcgcct
      181 ttcgcaagat cgataagtcc ttcgtcgaag ctgtaacgca tcgctcgcca gcggttttcg
      241 cggatcagca tggtcgtgta caggcgccag cgctggttcg cgaggcgtaa ccggtacagc
      301 atgcggacga tggagatgag cagtgcgaca atcgcgacgc agtcgtcgag ccgcgtgcag
      361 acgtccatca cccgcacttc gatggtcggg aaccgcccgc tggggcgcag atcccaccag
      421 atcttggtcg agtcctcgat cagcccggcg ttgatcagca caccgacatg ccgctggtac
      481 tcggcgtaac tctggaagcg ctccgggatg ccggtccgcg gtaacgcatc aaagatggtc
      541 agccggtaac tcttgaggcc ggtgtcccgg ccctcccaga atggagacga gcaggaaaag
      601 gcgagcagat gcggcaggaa atagctcatc tggttcatca gatcgatgcg cagctcctcg
      661 tcgctgatgc cgacgtggac atgcatgccg cagatgacga gtcggcgcgc ggcgccctgc
      721 atctcggcgg tgagcgaatc gtatcgatcc tttgccgtct gcttctgttc cgaccagcgg
      781 ctgaacgggt gggtcgaggc ggcgatcggt gccaggttgt gacggcccgc cacctcaatg
      841 atgccgctgc gcaagcgtgc gagatccgct cgcacctcct gcacggttct gcacaccttc
      901 gtgccgactt ccacctggga gcgcagcagc tccgacgtca cctggccgcc gcagcgctct
      961 tcgcattcgc cgatcaggga ttgcggcggg tcgatgatga gcgcccgcgt tccgcggtcg
     1021 acgagcaggt attcttcctc gacgccgatc gtaaactcgg gttccactgg tttcattgtc
     1081 gttgcgcctt cctgtaattc ccggccgccg gcaagccgaa gatcgacgtc ggcaggtcgt
     1141 cgatgatccc gtgcaggatg cccgctaccc tggcaacgcc agcgtcgttc gaaatcaggt
     1201 cctgccgtac ctcgattccg gcgtgcggca ggccggcggc ctccgcgtgg ttgtcgaccg
     1261 tgaagtcctg cggcgcacgg ccggagtacg gctcattatc gccgacgacg aagcctgagt
     1321 cggcgagttt tcgcatcaat atatccgcta tgcgcgggtc cgcgtcccac aggatgccga
     1381 actcccacgg ccgtgatgtg ccgtcgagca caggggtgaa actgtggatt gcaatcaccg
     1441 cgggcgatcg cccttcgccg gccagccgct tgatttcggc gtcgatagcg cgatggtaag
     1501 gccagtagat cgcttcggcg cgggcggcct tctgctcggg ctgcaggttc ctgttcccgg
     1561 gaatgacgat gccgtcacca aattcgagga atgccccggg gtcgagcagg tcgcggttgc
     1621 agtcgacgac caggcgcgag taccccgcca gcacggcggt cgcaccgaga ctttcggaga
     1681 gtttcgccgt cagggcgccg gcaccgatgt cgagcgcgag gtggcaacgg cgcgcgagcg
     1741 gatccaggcc aagatccccc agcgcccgcg ggaacacacg gctcgcgtga tcgcacacga
     1801 gcaagagcgg cttcccggcg gacgggttca ggatggaaaa aggcgcgggc tcgtctgcgg
     1861 caagaaggtc agtaacagcg ggatgtccgg cagtgcctcg tggcggcatg gtaaacggca
     1921 ggtaagtcaa aggcggcaag tgtaacctgt ttcgcagaaa ccacgacggt gttgaatcag
     1981 gcactgtagg agcccgccgt gcgggcgagt ttgttgagcg ttatcgaact cgcccgcatg
     2041 gcgggctcct acgggtgcca atcgacccgg ccgacgaacg tcgacatcag ggcatcgagg
     2101 gacatcctcg caacgtcggg ccgcccgcat ggccaactcc tgcagcaggc ttcaggcgag
     2161 ccgcgaggca gtggcgtcgc tcgcccaggg ttcgaaggtc tgtaccgagg tggggcgcgc
     2221 gatgaagaag ccctgtgcga actcgatccc taggtctccc agcttgtcga ggactttctt
     2281 cgattccacg cgttccgcga tcgtcgcgat gcccatcgcg cgtccgactt cgttgatggc
     2341 gcgcaccatg ctctcgtcga cggaatcttc ggcgacattg cggatgaact ggccgtcgat
     2401 cttcagatag tccaccggca ggttcttcag gtacgtgaac gacgagagcc cgctgccgaa
     2461 gtcgtccagg gagaacttgc agccgagctt gcgcagcgtc tgcatgaaat gaatcacgcg
     2521 cgagaggttc gatatcgccg cagtttcggt aatctcgaag catatcgcgc cttgcggcag
     2581 gcgctgcttc gtcagttcct cgatgacgaa ctcgaggaag cggtcctcgc tcaacgatgt
     2641 gccggacagg ttgatcgcca gcgtgaaccg cgcctgctcg gaagtatgac tgcggtcggc
     2701 aagctgcgac agcgcttcgc gaattaccca gcggtcgagc atcgacatca ggttgtagcg
     2761 ttccgccgcc gggatgaact ggtcgggccc cactatctgg ccgtcctcgc cgcgcatccg
     2821 cagaagcagc tcgtagtgcc cgcgggcacc ggcgcccttt tccccgatgc cgatgatcgg
     2881 ctggtagaac agttcgaagc ggttgtcctc gacggcgctc gtaatccggg acacccactt
     2941 catttcctcg tgacgcaccg acgcgtcgcg gtcctggtac aggtggacct ggttgcggcc
     3001 catgtccttc gcggagtagc aagccacgtc ggccgaactc atgatgcttg cgacactctg
     3061 ggtgtccttg ttgaccatca cgatgccgat ggagcagcgc acgctcgtgt acgcctcctg
     3121 ccaggtgaaa cggaacgctt cgacagcgct gcggatcgcc tccgacacct cgatggcgcg
     3181 ttcctcagtg caacgctcga gcaggatgcc gaattcgtcg ccgccgagcc gcgcgagtac
     3241 gtctgtcgac cggatgttcg attgcaccag ctcggaaatc tggcgcaaca actcatcgcc
     3301 cgcggtgtgg ccgaaggtgt cgttgacgac cttgaactgg tccaggtcga agtagagcag
     3361 cgcgtgcgaa gcgccctggc cgtaggcttt gtccagcgcc gcgaccaggc ggttctcgaa
     3421 ctcgcggcgg ttgatgaggc cggtcaatgc gtcgtgtgac gcctggtagg acaactgccg
     3481 gaacagcctg gtttccttgc tgacgtcgtg gaacaccatg accgcaccga tgatattgcc
     3541 gatgcggtcc cggatgggcg cagcggaatc ctggatagcg atctctacgc cctgctggtt
     3601 gatcaggacc gagtggtccg cgagcgcgat gaccctgccc tccttcaggc agcggagcac
     3661 cgggttttcg accgtggcgc gcgtgtgttc gttgacgatt gtcatgatct tgtcgatcgg
     3721 cttgccgcgc gcattacgta tttcgagccc cgccagttcc tgcgccacgg ggttgatgta
     3781 atccacgcgc ccttcggcgt cggtcgtgat gacgccgtcg ccaatagact gcagggtgac
     3841 ctgggcacgt tctttctctt cgaatacccg cgtctcggca atttttcttt cggtaatgtc
     3901 cgtgatcgtg ccttcgtgcg caacgacctt gccgtcgtca tcgtaaattg cacgggagtt
     3961 ctccagcacc acgatctcgc gcccgt
//
LOCUS       DASUOM010000046         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1789733,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000046 DASUOM010000000
VERSION     DASUOM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789733"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFY27_00970"
     CDS             <1..273
                     /locus_tag="VFY27_00970"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5764109.1"
                     /translation="MLEGIVLFVILWTFSSRPRPYMALTGMFLLWYGVFRFFVEFFRL
                     PDADIGYLAFGWVTMGQVLSAPMIIAGALMLLMAYRNGPAGDRVAA"
     gene            275..1069
                     /locus_tag="VFY27_00975"
     CDS             275..1069
                     /locus_tag="VFY27_00975"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5764110.1"
                     /translation="MKQYLAMMRHVREHGAEKGDRTGTGTLSVFGYQMRFDLGEGFPL
                     VTTKKLHLRSIIYELLWFLRGDSNIEFLKRHGVSIWDAWADEQGELGPIYGVQWRSWP
                     APEGRTIDQIADVVQRLGENPDSRRMIVSAWNVSELERMALPPCHCLFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGFRVGEFIWTGGDCHLYRNHLEQADV
                     QLRREPYALPRLVIGRRPESLFDYRFEDFEIIGYEAHPHLRAEVAV"
     gene            1086..1595
                     /locus_tag="VFY27_00980"
     CDS             1086..1595
                     /locus_tag="VFY27_00980"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5764111.1"
                     /translation="MSKVTMVVAASANNVIGRRGELPWRLPEDLRHFKELTMGKPIVM
                     GRRTFESIGRPLPGRRNIVVSRRASLHLEGCEVSDSPDAALDLARQAEEIMVIGGQQL
                     YEALLPRTTRIHMTRVQETIEGDAFFPELPRDKWQVVWSELHPATREQPLAYTFEVLE
                     RAASAEVAL"
     gene            complement(1664..>2849)
                     /locus_tag="VFY27_00985"
     CDS             complement(1664..>2849)
                     /locus_tag="VFY27_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5764112.1"
                     /translation="ERAGVFPLVEKLLMPMRGRVIHSEDGATELQPYGQRRGEQIYSV
                     SRAELNRILIQAAEDCGIAVRFEQEARDFDGAGRGIMITDHADGSDWTCPDTPVIAAD
                     GAGSAMRRALDDAIGIAATESLLPHGYKELCIPPQPGGKHLLDPNGLHIWPRGGFMLI
                     ALPNPGGDFTLTLFLPNEGRESFASLSSEAAVEAFFCRYFADVVPLIPGLVESFLTNP
                     VGILGTVRCDGWHEGGRLLLIGDAAHAIVPFHGQGMNLAFEDCVVLDEAARTARTWEA
                     AFAAFEERQLPNANAIADMALENYVEMRDTVRDPKYKLRKELSFELERRLPDRFIPRY
                     SMVMFHEEIPYAVAQQRGAVQQALLDELVHDALALEEIDLSTAEELVRRQLPRLSLNK
                     PL"
BASE COUNT          525 a          869 c          897 g          558 t
ORIGIN      
        1 atgctggaag gcatcgtgct gttcgtcatc ctgtggacct tctcgtcccg cccgcgcccg
       61 tatatggcgc tcaccgggat gtttctcctg tggtacggag tgttccgttt cttcgtcgag
      121 ttcttcaggc tgccggacgc cgatatcggc tacctcgcct tcggctgggt cacgatgggc
      181 caggttctca gcgcgccaat gatcattgcg ggagccctca tgctgctcat ggcctatcgc
      241 aacgggcctg ccggagacag ggtagcggcc tgatatgaaa cagtaccttg cgatgatgcg
      301 tcacgtgcgc gagcacggcg cggaaaaagg ggaccgcacc ggtaccggca cgctcagcgt
      361 gttcggctac cagatgcgct tcgatctcgg ggaaggattc ccgctggtga ccacgaagaa
      421 gctgcacctg cggtcgatca tttacgagct gctgtggttt ctccggggcg attccaatat
      481 cgaattcctc aagcgccacg gagtttcgat ctgggacgca tgggcggacg agcagggcga
      541 actcgggccg atctacggcg tgcaatggcg gagctggccc gcgccggagg gcaggaccat
      601 cgaccagatc gccgatgtcg tgcaaaggct cggcgagaat ccggattcac gccgcatgat
      661 cgtcagcgcg tggaacgttt cggagctgga gcgcatggcg ctgcctccct gccactgcct
      721 gttccagttc tatgtcgcgg acggcaagct gtcgtgccag ctgtaccagc gcagcgcgga
      781 catttttctc ggcgtgccgt tcaacattgc ctcgtacgcg ctgctgacgc acatgatcgc
      841 gcagcagacc ggcttcaggg tcggtgagtt catctggaca gggggcgatt gccacttgta
      901 ccggaatcat ctcgaacagg cggatgtgca gctaaggcga gagccctacg cgctgccgcg
      961 gctggtgatc ggccgccggc cggaaagcct cttcgattat cgtttcgaag acttcgagat
     1021 catcggctac gaggctcatc cgcaccttcg cgccgaagtt gctgtatgaa ctgcccgaac
     1081 gccgggtgag taaggtgacc atggtggtcg cagcatcggc caacaatgtc atcggcagga
     1141 ggggcgagct gccgtggcgc ctgcccgagg atttgaggca cttcaaagag ttgacgatgg
     1201 gtaagcccat cgtgatggga cggcgcacgt tcgagtcgat cggccggcct ctgccggggc
     1261 gccgcaacat tgtcgtcagc cgtcgcgcaa gtttgcacct cgaaggttgc gaagtgagcg
     1321 actcgccgga tgcggcgctt gatcttgcga ggcaggcgga agagatcatg gtgatcggcg
     1381 gccaacagct ctacgaggcg ttgctgccgc gaacgacgcg catccacatg acacgcgtac
     1441 aggaaaccat cgaaggcgat gcgtttttcc cggaattgcc gcgggacaag tggcaagtag
     1501 tatggtctga gcttcacccc gcgacgcggg agcaaccact cgcatacacc ttcgaagtcc
     1561 tcgaacgcgc agcctccgcg gaagtagcgc tgtaggatcc catcccggcg ggcgacagga
     1621 cccttctcag ttgcgaaacg aatgtcgccc gcgggatgga ctcctacagc ggtttgttca
     1681 gggaaagacg cggcagttgt ctgcgtacga gctcttcggc cgttgaaagg tctatttcct
     1741 cgagcgccag cgcatcgtgc accagttcgt cgagcagcgc ctgctgcacc gcgccgcgct
     1801 gttgcgcgac ggcatagggg atctcttcgt gaaacattac catcgagtag cgcggtatga
     1861 acctgtccgg caggcgacgc tcgagctcga aggaaagctc cttccgcagc ttgtatttcg
     1921 ggtcgcgtac cgtgtcgcgc atctcgacat aattctcgag cgccatgtct gcgatggcgt
     1981 tggcgtttgg cagttgtcgc tcctcgaatg ctgcgaacgc tgcttcccag gtccgtgcgg
     2041 tcctggctgc ctcgtcaagg acgacgcagt cctcgaacgc caggttcatg ccctgtccgt
     2101 gaaacggaac gatggcgtgc gcggcatccc cgatcagcaa cagcctgcca ccctcatgcc
     2161 aaccgtcgca gcgcactgtt ccgaggatac cgaccgggtt cgtgaggaag gattcaacaa
     2221 gaccgggtat cagtggcacg acatccgcga aatacctgca aaaaaacgct tcaaccgcag
     2281 cctcgctact tagcgacgca aaactttccc tgccttcgtt gggcagaaag agcgtcagcg
     2341 tgaagtcacc gccagggttc ggcagcgcga tcagcatgaa gccgccgcga ggccagatgt
     2401 ggaggccatt cggatccagc aggtgtttcc cgcctggttg cggcggtatg caaagctcct
     2461 tatagccgtg gggcagcagg ctttccgtgg cggcgatgcc aattgcatcg tccagcgccc
     2521 gccgcatcgc tgagccggca ccatcggcgg caatgacggg cgtatccggg cacgtccagt
     2581 cggagccgtc ggcgtgatcg gtgatcatga ttccccgacc ggccccgtcg aagtcgcgcg
     2641 cctcctgctc gaagcggacg gcgatgccgc agtcttctgc agcctggatc aggatccggt
     2701 tgagctcggc tcgcgatacc gagtaaattt gttcgccgcg gcgttgcccg tatggctgga
     2761 gctccgtagc tccgtcttcg ctatggatga cgcgtccccg catcggcatc agcaacttct
     2821 cgaccagcgg gaacacaccg gcgcgctcc
//
LOCUS       DASUOM010000047         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_825501,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000047 DASUOM010000000
VERSION     DASUOM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825501"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..273)
                     /locus_tag="VFY27_00990"
     CDS             complement(19..273)
                     /locus_tag="VFY27_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764113.1"
                     /translation="MAASCGPIATTKTSRIFSPCTYDISTIYAALGDVDETFSWLERA
                     LEERSQLIGWLPWDPVFDGIRSDPRYDNLVRRLNLPASPE"
     gene            complement(335..661)
                     /locus_tag="VFY27_00995"
     CDS             complement(335..661)
                     /locus_tag="VFY27_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764114.1"
                     /translation="MRYFIYSIDFHSHFWQFKASANRDNAAGGLLQPRNRAGAAQTLA
                     VFPFRPLTATDRNESLELGMAETLITRLNDSRLLVKPLSAVRRYANPGGDAVAAGREL
                     NVDAVL"
     gene            797..961
                     /locus_tag="VFY27_01000"
     CDS             797..961
                     /locus_tag="VFY27_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764115.1"
                     /translation="MADFWKDDRGEHAIEFLMILTFGVLPLIGAVFLLEDVLREYVSF
                     GQIFISSPFF"
     gene            1017..1247
                     /locus_tag="VFY27_01005"
     CDS             1017..1247
                     /locus_tag="VFY27_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764116.1"
                     /translation="MNNLAYTAGLGLPEFCNLLTRLHEDERGDNENLGRLLILALVLI
                     PLIILIAIFGADIAKRARDAWDSLIGKEVKQQ"
     gene            1288..1827
                     /locus_tag="VFY27_01010"
     CDS             1288..1827
                     /locus_tag="VFY27_01010"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5764117.1"
                     /translation="MTLSHFAAIGLVTAAAFFDIARHRIPNLLTYPSWALGLALGAYA
                     AGMDGMTNAALGFAAGFFPLLLMFMSGSLGGGDVKLMGGVGALLGFPAGLNALISSIL
                     VGGFFAAIILLWQGRLIPILKYAASRLWSKVWTVHVPLPAPPEHKDAFPFGVAIALGT
                     YLTLISIQMGAQTPNNLFS"
     gene            1845..2774
                     /locus_tag="VFY27_01015"
     CDS             1845..2774
                     /locus_tag="VFY27_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX5764118.1"
                     /translation="MYIDIAYADRLAELTFEARAWWCIAVGSLLMLSVVVVRAGRRCS
                     SQVEVNPGAAVPASLLKRLHRGESATASMEYLLVLFPFLIIVMTVWQLAFMINAQMHV
                     AYAAYGAARSASVVISTELTNEKEGLLKKQDDAGATKWTRIRDAAIPGTLAISPGDFG
                     TAGLVALASSVRRGGVGNLMQLPDATAIIPRFTLMTVHHTDPGIFSGTRLQRGLVKNA
                     YAEKMTQVLVNKQDHTKTQDLAGASRIEVTVNYIFWLHVPYVGRLMEAMFEGYQNPLT
                     GEYILLNPYPSMQLSETISMTNWPRRRAIEPCT"
     gene            2765..>3119
                     /locus_tag="VFY27_01020"
     CDS             2765..>3119
                     /locus_tag="VFY27_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX5764119.1"
                     /translation="MYLAALNLVKRLHREESAQISFLAVAGVICFVGLLSMVINTNDV
                     VTERVHMQDVADATVLSAASWTARGLNTISFVNVLNTKLISTAVLLNALADSIPVMEA
                     VGQIQQAIFSGCSGVP"
BASE COUNT          591 a          929 c          959 g          640 t
ORIGIN      
        1 gccgggcgac aaaacggctc attccggcga tgccgggagg ttcagccggc gcacgaggtt
       61 gtcgtaccgc gggtccgatc gtattccgtc gaacacaggg tcccacggca gccagccgat
      121 cagttgcgat cgttcctcga gcgcgcgctc gagccaggag aaggtctcgt ccacgtctcc
      181 aagcgccgcg tagatcgtgg agatgtcgta tgtgcacgga gaaaatatcc gtgaagtttt
      241 cgtcgtagcg atcggcccac agctggcggc catcgcggac gtcaatcagc cgcgcggaaa
      301 cccgcaggcg acctccggct cgctgcaagt gaccctacag caccgcatcc acattaagtt
      361 cgcgccccgc agcgaccgcg tccccgccgg gattcgcgta gcgccgcacc gcactcagcg
      421 gcttgacgag cagccgcgaa tcgttcaggc gcgtgatgag cgtttcggcc atgccgagtt
      481 ccagcgactc gttgcggtca gtcgccgtga gcggacggaa cgggaatacg gcgagcgtct
      541 gcgccgctcc tgcccggttg cgcggttgca gtagcccgcc ggcggcgtta tcgcggttag
      601 cgcttgcctt gaattgccag aaatgcgaat ggaaatcaat cgaatagatg aaatatctca
      661 agtcgatttc actgatttat cgagaccgct attgcgttgt ccggcggctg gattgatatt
      721 agcgacgcag tacgcacgtg cattgtgaca acaaactccg agaacggcgg tgcactcaga
      781 ccaggcgcga agaacgatgg ctgatttctg gaaggacgat cgcggcgagc atgcgattga
      841 attcctgatg atcctcacgt tcggcgtgct ccccctgatc ggcgccgtct tcctcctgga
      901 agatgttctc agggaatatg tttccttcgg acaaatattt ataagttccc ctttcttcta
      961 gttcgtcgaa aggggggaat aacaactcag aaagaaacgc gcacagaggg agtgacatga
     1021 acaatttggc ctatacggcc ggcttggggc tgccggaatt ctgcaacttg cttacccgcc
     1081 tgcacgaaga cgagcgcggc gataacgaaa acctggggcg actccttatt ctcgccctgg
     1141 tgctcatacc gctcatcatc cttattgcga ttttcggcgc cgacatcgct aaacgagcca
     1201 gggatgcctg ggattcgctg atcggcaaag aagtaaagca gcaataggca ggctgatgct
     1261 ccgcccctcg gcagaggtgg ccttcgtgtg acgctttcac atttcgcggc tatcggactg
     1321 gtaactgcgg ctgcgttttt tgacatagcg cgccaccgga taccgaacct tctcacgtac
     1381 ccgtcgtggg cactgggact ggctctgggc gcctacgctg ccggcatgga cggaatgacg
     1441 aacgcggccc tgggctttgc tgcaggtttc ttccccttgc tgctgatgtt catgagcggg
     1501 tccctgggcg gtggtgacgt caagttgatg ggcggggtcg gagcgctact ggggttcccg
     1561 gccgggctga acgccttgat ctcctcgatc ctggtcggcg gcttcttcgc cgccatcatt
     1621 ttgctctggc agggcaggct cattccgata ttgaagtacg ccgcctccag gctgtggagc
     1681 aaggtgtgga ccgtgcacgt gcccttgcct gcgccgccgg agcataagga tgcgttcccg
     1741 ttcggcgttg ccatcgcgct ggggacttac ctcacgctga tatccatcca gatgggtgcg
     1801 cagacgccaa acaacctgtt ttcctgagct gaacgacaac ggccgtgtac atcgacattg
     1861 cctacgcaga cagactcgca gagcttacgt tcgaggctcg cgcgtggtgg tgcatcgccg
     1921 tgggttcgct tctcatgctg tccgtcgtcg tggtccgggc gggcaggcga tgctcgtcgc
     1981 aggtcgaagt caaccctggc gcggctgtac cagcctccct gttgaaacgc ctgcaccgcg
     2041 gggaatccgc caccgcgtcg atggaatacc tgctggtgct gtttccgttc ctgatcatcg
     2101 tcatgacggt gtggcagctc gcgttcatga tcaacgcgca gatgcacgtc gcttacgcgg
     2161 cctatggtgc ggcgcgcagc gcgtccgtgg tgatttccac ggaactcacg aacgagaagg
     2221 agggcttgct gaagaagcag gacgatgcgg gcgctacgaa gtggacgcgt atccgcgatg
     2281 cagcgatacc cggcacgctc gccatctcgc cgggtgattt cggcacggcg ggactcgtag
     2341 cactcgcatc cagcgtgcgg cggggcggcg tcggcaacct gatgcagctg cctgacgcca
     2401 cggcgatcat cccgcgcttc acgctgatga cggttcatca cactgatccg ggtattttct
     2461 ccggcacccg gctgcagagg ggcctggtga agaatgcgta cgcagaaaaa atgacccagg
     2521 ttctcgtcaa caagcaggat cacacgaaaa cgcaggacct ggccggtgcc agccggatcg
     2581 aggtgacggt caattacatc ttctggctgc acgtgccgta cgttggccgc ctgatggaag
     2641 ccatgttcga gggctaccag aacccgctca cgggcgagta catactgctg aacccgtacc
     2701 cgtccatgca gcttagcgaa acaatcagca tgacgaactg gccccgccgg cgggcgatcg
     2761 agccatgtac ctagcagcac tgaacctggt gaagcgcttg caccgcgagg agtccgcgca
     2821 gatctccttt cttgcggtgg ccggggtgat ctgtttcgtc ggactattgt cgatggtcat
     2881 caatacgaat gacgtcgtga cggagcgcgt gcacatgcag gacgtcgccg atgcgacggt
     2941 gctgtcggca gcctcctgga ctgcgcgggg actgaacacg atctcgttcg taaacgtgct
     3001 gaacaccaag ctcatctcga ccgcggtcct gttgaacgca ctggccgatt cgatccccgt
     3061 catggaggcc gtgggccaga tccagcaggc cattttctcg ggttgctccg gcgtgcctt
//
LOCUS       DASUOM010000048         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_824916,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000048 DASUOM010000000
VERSION     DASUOM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824916"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1156
                     /locus_tag="VFY27_01025"
     CDS             179..1156
                     /locus_tag="VFY27_01025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5764120.1"
                     /translation="MKAVLCRELGPPALLAVEEVPSPPLANDQVRVRVRACGVNFPDV
                     LMVAGKYQLQPPMPFSPGAEFAGEIITVGEDCRNSTVGQRVLAITGFGGMAEEASVAE
                     SSVVPIPLDLEYETAAAFLLTYGTSWHALRQRAALVPGETLLVLGAAGGVGLAAVQIG
                     KLLGAKVIAAAGTPEKLQLARANGADEIVNYTEARLKDAVRELTSGRGADVIFDPVGG
                     ELFGDCLRAVAWRGRILIVGFASGSIQSIPANLPLLKGSSLLGVFWGQFTKREPQLHQ
                     QNTAELLAALQQGRLKPHIGAVFPLERAADALQMLVERRAAGKVVVRVS"
     gene            1559..>2002
                     /locus_tag="VFY27_01030"
     CDS             1559..>2002
                     /locus_tag="VFY27_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5764121.1"
                     /translation="MQMYSEKDVDSRALAGQRVAILGYGSQGRAHALNLRDSGHDVVV
                     GLRRDGASWKKAARDGLEVREPAQAVEGAAIVAFLTPDMTQASLYQSVEPALPDTSAI
                     LFAHGFNIHFRQIEPRADLDVILIAPKGPGDLVRRQYEEGHGVPCL"
BASE COUNT          407 a          609 c          620 g          366 t
ORIGIN      
        1 tgaaatccat ctatggttta ctcttgagcg agctgcaaac agaaatcaca atgccgaatg
       61 ccgcatcagg gttccgcatg gccgcagcat acctgtatat aaggaatggc cgctacgcgc
      121 ctgaccatga cctgccttcg acggaactgc cggccgatcc acagcggggt cgttgattat
      181 gaaagcagta ctgtgcagag aactcggtcc gcccgcgctg ctggctgtgg aagaggtgcc
      241 ctcaccgccg cttgccaacg accaggttcg ggtcagggtg cgcgcctgcg gcgtcaactt
      301 ccccgacgtg ctgatggtcg ccggaaaata ccagttgcag cctccgatgc cgttcagccc
      361 cggcgcggag ttcgcgggcg agatcattac cgtcggcgag gactgccgca actcgaccgt
      421 cgggcagcgc gtgctcgcca tcaccgggtt cggcggcatg gcggaggaag cttcggtcgc
      481 cgagagttcc gtagtcccga tcccgctcga cctggagtat gaaaccgccg cggccttcct
      541 tctgacgtac ggcacctcat ggcacgcact caggcaacga gccgcactcg tccccggcga
      601 gacgctgctg gtcctgggcg cggcaggcgg ggtcgggctt gccgcggtgc agatcggcaa
      661 gttgctggga gcgaaagtca tcgccgctgc cggcacgcct gaaaagcttc agctggcccg
      721 cgcgaacggc gcggatgaaa tcgtcaacta cacagaagca cggctgaaag acgccgtacg
      781 cgaactgaca tccggccgtg gcgccgacgt tattttcgat cctgtcggcg gtgaactgtt
      841 cggcgactgc ctccgtgccg tcgcgtggcg agggaggatc ctgatcgtcg ggtttgccag
      901 cggcagcatc caatccattc ctgcaaatct tccgctcctt aaaggcagtt cgctgctcgg
      961 ggttttctgg ggacaattca caaagcggga accgcagctt catcagcaaa acacggccga
     1021 gctgctcgcg gcgcttcagc aagggagact gaaaccacac ataggtgccg tctttccgct
     1081 cgagcgtgcg gcggacgcac tgcagatgct cgtggagcga cgagccgcgg gcaaggtcgt
     1141 cgtccgcgta agctaaaatt tttgcgtgag gcgggagccc gcatccgggc gagtgcgatt
     1201 tgcgccggcg aactcgcccg catgacggct cctacggttg cagaagtaac tgaaacatcg
     1261 gcggttgaca tcgttccgtt catccaacat gatcgagcgc tatgaacgtc ttgacgtccc
     1321 ctgccctgct tcgtccggtc cgcatcagca gcctccttcg tgtgaagctg gtgctcctcc
     1381 ttggcgggag aaagagcggg tagcgaacca aaattaaacg aagacgcaaa ccccgcattc
     1441 cgacaggagc gtggggtttt tttttggcgg gagtaatccg cgggattaag caggactgat
     1501 aaagcaagag aagagcacga tcacgaacct ttgtaagcac atcggggaca acaaccaaat
     1561 gcagatgtat tcagagaaag acgtcgactc cagggccctt gcaggccaac gcgtcgcaat
     1621 cctgggctat ggcagccagg gtcgcgcgca tgcgctcaac ctgagagaca gcggtcatga
     1681 cgtggtagtc ggcctgcgcc gcgacggcgc cagctggaag aaagctgcca gggacggcct
     1741 cgaagtacgg gagccggcgc aagcggttga aggtgccgcc attgtcgcgt tcctgacacc
     1801 cgacatgacg caggcgtcgc tgtaccagtc cgtcgagcct gccctgccgg ataccagcgc
     1861 cattcttttt gctcacggct tcaacattca cttcaggcag atcgagccac gcgcagacct
     1921 cgatgtgatc ctgatcgcac ccaagggacc aggcgacctc gttcgccggc agtacgagga
     1981 aggacatggc gttccgtgcc tg
//
LOCUS       DASUOM010000049         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_462834,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000049 DASUOM010000000
VERSION     DASUOM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462834"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFY27_01035"
     CDS             <1..389
                     /locus_tag="VFY27_01035"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764122.1"
                     /translation="RACAEGKNGFEQAFLYIRYHVEVMVGERGPVAIMSEIPSLKDAH
                     REEILQISRQHSSAFEKIIEEGIEDGSVGLCDVRMTGNAIMGAINWIPKWFHGDAETA
                     ERILAEFPALLTRCLLPAGSGAAGRG"
     gene            complement(407..1162)
                     /locus_tag="VFY27_01040"
     CDS             complement(407..1162)
                     /locus_tag="VFY27_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764123.1"
                     /translation="MNKFSYRRLYLAFASRQRIGAGAVAILLATAPLAAPADEYRQFP
                     GAQTDSRTLSVQERVEDVFSSGDYGRALLIYEKELAPLGDKYAQYMVGFMHLTGKGVA
                     ENPAEALAWYRLAAERGEVPFVKARDALEGSLQPDELTRSNSRFAELWQSYGDRKLLL
                     DLISEDLEILRQYEASPDGVANAGTRVALAAGYAGTETTGSYHRRVRTRLAERLEYLS
                     STKGAAEAGIDDPGILEELESQVRQDLKVLELN"
     gene            complement(1318..2115)
                     /locus_tag="VFY27_01045"
     CDS             complement(1318..2115)
                     /locus_tag="VFY27_01045"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5764124.1"
                     /translation="MSDRVEISIEKHVALVRMNRPKKHNALDHESFEALIEAGETLAR
                     NKSVRAVVLAGAGESFCAGIDMSVFDGDRLAAAREELLPRGETCANFYQRAALVWREL
                     PVPVIAAVHGVAYGAGLQIAMAADVRVAAPSARLSIMEVRWGIIPDMGLSVTMRHVVP
                     ADRIRMLAYTGKIVDGTEALKLGLITELAGDPLMEAGLLATAISQRSPDAIRAIKALL
                     NESLDEQAPDALRREADLQRSLLGSPNNREAVMANLHKREPHFQNPQ"
     gene            complement(2157..>2856)
                     /locus_tag="VFY27_01050"
     CDS             complement(2157..>2856)
                     /locus_tag="VFY27_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5764125.1"
                     /translation="LKTVPTFASMLPPAAFLAESGLDGAKILLAGLRLELYRPLPAAA
                     NLLVNRRVTGVYDKGAAHGAVIVVQSEARLAKDETALFTLGSTLIARGDGGIGGPRAA
                     PLERHALPKRDPDLSCSSPTRADQALLFRLSGDRNPLHADRAAAREAGFPRPLLHGRC
                     TYGIACRAILNTICDYDHTLITGFEARFSAPVYPGDTLTTEMWQDGNIVSFRCSVKAR
                     NSIVLSNGKCTLAA"
BASE COUNT          499 a          904 c          916 g          537 t
ORIGIN      
        1 agcgggcctg cgcagaggga aagaacggct tcgagcaggc attcctctat atccgctatc
       61 acgtggaagt gatggtgggg gagcgggggc ccgtcgcgat catgagcgag attccgtcac
      121 tcaaggacgc gcacagggaa gaaattctgc agatctcacg gcagcacagc tccgcattcg
      181 agaaaatcat cgaggagggt atcgaagacg gcagtgtggg cctttgcgat gtccgcatga
      241 ccggcaacgc gatcatgggc gcgatcaact ggattccgaa gtggttccac ggcgacgcgg
      301 aaacggctga acgaattctc gcggaatttc cggccttgct tacgcggtgc ctgctgccgg
      361 cgggatcggg cgcggccggg cgcggatgac gctccgggcg gcgctatcag ttgagctcta
      421 gaaccttcag gtcctggcgc acttgcgact ccagttcttc caggatcccg gggtcatcga
      481 tgccggcttc cgccgcacct tttgtggagg acaaatactc cagccgctcg gcaagcctcg
      541 tgcggacccg gcgatggtag ctcccggtcg tctccgtacc ggcgtatccc gcggccaggg
      601 ctacgcgcgt cccggcgttg gcgactccgt caggcgacgc ctcgtactgc ctcaggatct
      661 ccagatcctc gctgatgagg tccaggagga gtttccggtc gccatagctc tgccatagct
      721 cggcaaaccg tgaattcgac cgcgtgagtt cgtcgggctg cagcgatcct tccagagcgt
      781 cgcgagcctt gacgaacggc acctccccgc gctctgcggc gagccggtac cacgccagcg
      841 cctcggcggg gttttccgca acacccttgc ctgtcaggtg catgaagccg accatgtatt
      901 gcgcgtactt gtcgccgagc ggtgcgagtt ccttttcgta gatcagcaac gcccggccgt
      961 agtcgccgga cgagaacacg tcctcgaccc gttcctggac gctgagcgtg cggctgtccg
     1021 tctgtgcgcc cggaaactgc cggtattcgt ctgcgggcgc agccagcggt gcggtagcga
     1081 gaaggattgc aacggcgccc gctccgatgc gctgcctcga agcaaaggcc aggtagaggc
     1141 gacggtaaga aaatttgttc aagcagacct catgagcgtg cagcggctgg tttgcatcgc
     1201 ggcgcgccgg ttatcgtttc gctggttgtc catgcggacg gtcatacgag tgagactgcc
     1261 gcctcacgcc gggagttcct tgccggggca agacattcga tggccgcact tccacgccta
     1321 ctgcggattc tggaagtgcg gttcgcgctt atgcaaattt gccattaccg cttcgcggtt
     1381 attgggtgat cccagcagcg aacgctgaag gtctgcttca cggcgcaatg cgtccggcgc
     1441 ctgttcatcc agcgactcgt tcaggagtgc cttgatcgcc cggatggcgt ccggagagcg
     1501 ctgcgatatc gcggtcgcca gcagaccggc ctccatcagc ggatccccgg ccagttccgt
     1561 gatcaatccc agcttcagtg cctccgtgcc gtcgacgatt tttcccgtgt acgccagcat
     1621 gcggatccgg tccgcgggta cgacatgtcg catggtgacg ctgagaccca tgtccggaat
     1681 gattccccag cggacttcca tgattgaaag cctggcggac ggcgcggcga cgcggacgtc
     1741 ggctgccatc gcaatttgca gtcccgcgcc ataggctacg ccatgtacag ccgcgatgac
     1801 gggaaccggc aactcacgcc agacgagtgc cgcacgttga tagaagtttg cgcaggtctc
     1861 gccgcgtgga agcagttctt ctctggccgc agcgaggcga tcgccatcga acaccgacat
     1921 atcgatgccg gcgcaaaagc tttcgccggc acctgccagg acgaccgccc gcacggactt
     1981 gttccgggcc agcgtctccc ccgcttcgat caaagcctcg aaggattcgt gatcgagggc
     2041 attgtgcttt ttcggccggt tcattcgcac cagcgccacg tgcttctcga tgcttatctc
     2101 cacacggtcg ctcatcggct cgctcactcc atatcaggtt gcgggacgct ccgggcctac
     2161 gccgccagcg tgcactttcc gttgctgagc acgatgctgt tgcgcgcctt tacggagcag
     2221 cgaaacgata cgatgttccc gtcctgccac atctcggtcg tcagcgtatc acccgggtag
     2281 acaggcgcgg agaaccgcgc ctcgaagccg gtaatgagcg tgtggtcgta atcgcagata
     2341 gtgttgagga tcgcgcgaca ggcgattccg taggtgcagc ggccgtgtaa caggggccgg
     2401 gggaaacccg cctcgcgcgc cgcggcccga tcggcatgca gcggattgcg atcgcctgac
     2461 aggcgaaaca gaagtgcctg gtccgcgcgc gtcgggctgg agcagctcag gtcgggatct
     2521 cgcttcggca gcgcgtgccg ctcgagcggc gcagcgcgag ggccgccgat gccgccgtcg
     2581 cccctggcga tcagcgtgct gccgagcgta aacagggcgg tttcgtcttt cgcaaggcgt
     2641 gcctccgatt gcacgacgat cacagcgccg tgcgcagctc ccttgtcata cacaccggtg
     2701 acgcgccggt tcaccagaag gttcgcggca gcaggcagag ggcggtacag ttcgaggcgc
     2761 agccccgcca gcaggatctt cgccccgtcg aggccgcttt cggcaagaaa cgccgcgggc
     2821 ggcaacatgc tggcaaaggt ggggaccgtc ttcagt
//
LOCUS       DASUOM010000050         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_824421,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000050 DASUOM010000000
VERSION     DASUOM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824421"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="msrA"
                     /locus_tag="VFY27_01055"
     CDS             <1..484
                     /gene="msrA"
                     /locus_tag="VFY27_01055"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5764126.1"
                     /translation="AGGSAQSARYELVGRGDTGHAESVQIVYDPTKITYGQLLQIFFS
                     VVHDPTQLNRQGPDHGPQYRTAIFTADPAQRELAKKYIAQLDAAKVYPKKIVTALEDA
                     PFYPAEAYHQDFLERNPNHPYISYHDLPKVRDLERVFPGVYRAEPVLVSPKRAVSIPR
                     "
     gene            complement(492..1130)
                     /locus_tag="VFY27_01060"
     CDS             complement(492..1130)
                     /locus_tag="VFY27_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX5764127.1"
                     /translation="MSITRESPWSLLHFGLLVLLALQAALIAARVLSSGADALLGAPL
                     GVMAATLVLAASNRRLAADPHIGALTGWLVALRGLVALVLVAATGALVFDRYVSATAP
                     DEFARGVCALLWLVLTIKGALIGKLKPNGVIGLRVPWTLESRLAWDKAHRTLGRALFW
                     VGLAGLASSLSIPPTISMPLIIAAVVSAVTLALIEARSAWRSDPQRGLRSAR"
     gene            complement(1127..1432)
                     /locus_tag="VFY27_01065"
     CDS             complement(1127..1432)
                     /locus_tag="VFY27_01065"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5764128.1"
                     /translation="MNTLFKALSHPVRREIVSMLQTGPMSSGDIAAAFDMAWPSVTGH
                     LNALKEADLIEPERDGNSIRYRLNISALEEAVSFLLGVVQPASEKRGATRKLKRRTT"
     gene            1847..2509
                     /locus_tag="VFY27_01070"
     CDS             1847..2509
                     /locus_tag="VFY27_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764129.1"
                     /translation="MNLSRTFCALLIGLAGFVCGCAETSTALQKAEAQPEQKAEPEQK
                     ADAQDQRRILNEGYSIFYSTVDGLSKFDLIFLGKSETESVYEVTTAVTEYADTLKATL
                     DRVAKDYPAVNMELDPLPVIEQRTRGGITTDRLLSFAPVVGLTGESFDRRMLQVLEGT
                     LNNLRFMAKALADEEPEPSLKQIATTAHNRFEQLYGQVLNMLDEKYFINTGERKVAKA
                     AP"
     gene            complement(2666..3736)
                     /locus_tag="VFY27_01075"
     CDS             complement(2666..3736)
                     /locus_tag="VFY27_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764130.1"
                     /translation="MSSQINPERENTDASLRVEREKTDQALVDRQVAVDENADQTVEQ
                     ARSVADAVVATARVTADELLRAGGDEHVLPHTVLAEQRAVEDDALQDARADADEALRQ
                     QREENVRILAALLPLERQKTDRYLLTERARADDAVAHRDNFLGIVTHDLRDLLSGIVL
                     SAALLPDSTSEPNGTKRRIERYAARMNRLIGDLIDVASIDAGQLKVVPALADWSALIE
                     EVAALFRPSASAQGITLEIALAERSLPMRFDHDRMLQVLANLIANSLKFTPRGGRIRV
                     EAARGADELRCAVTDTGSGIPAHMLEAIFQRFWQVRSDDRRGVGLGLYITRCIVEAHR
                     GRIWAESIEGLGSTLRFTLPIV"
     gene            complement(4243..4983)
                     /locus_tag="VFY27_01080"
                     /pseudo
     CDS             complement(4243..4983)
                     /locus_tag="VFY27_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
     gene            complement(5061..5303)
                     /locus_tag="VFY27_01085"
     CDS             complement(5061..5303)
                     /locus_tag="VFY27_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764131.1"
                     /translation="MDAIAIAAQIRERGSLLAAIEELRGDVKTLCPYQTPDLSAVEEW
                     LAKNDVLGPGGPDEPFEPLARRRLFRRSNRLASGEP"
BASE COUNT         1115 a         1703 c         1630 g         1051 t
ORIGIN      
        1 cgccggtggg tccgcgcaga gcgcgcgtta cgagctcgtc ggccgcggtg acaccggcca
       61 cgccgaaagc gtgcagatcg tctacgatcc gaccaagatc acctatggtc agcttttaca
      121 gatcttcttt tcggtcgttc acgatccgac gcaactgaac cggcagggcc ccgatcacgg
      181 tcctcagtac cgaacggcga tctttacggc cgatcccgcg cagcgcgagc tcgcaaaaaa
      241 gtacatcgcg cagctcgacg ccgcaaaggt gtatccgaaa aagatcgtga cggcgctaga
      301 ggacgctccg ttctatccag ccgaggcata tcatcaggac ttcctcgagc gtaacccgaa
      361 ccacccgtac atctcgtatc acgacttgcc gaaggtccgt gacttggagc gcgtgttccc
      421 aggtgtctat cgcgcagaac ccgtactcgt ttcgccgaaa cgggctgtca gcatcccgcg
      481 ctagcggagg tctaccgcgc ggaccgcaat ccgcgttgcg gatcgctgcg ccaggcggac
      541 cgcgcttcga tgagcgctag cgtcacggcg ctcacgactg cagcgatgat cagcggcatc
      601 gatatcgtgg gcgggatcga gagactactg gccaggcctg caagcccgac ccagaacagc
      661 gcgcgcccca gcgttctgtg cgccttgtcc cacgcaagcc ggctttccag cgtccacggc
      721 acgcgcagac cgatcacacc gttgggcttg agcttgccga tcaacgcgcc cttgatcgtc
      781 aggaccagcc acagcagggc gcaaacgccg cgcgcaaatt cgtccggtgc cgtcgcgctt
      841 acatagcggt cgaagaccag cgcgcccgtc gccgcgacga ggacgagcgc aacaaggccg
      901 cgcagcgcca ctagccatcc ggtcaaagcg ccgatgtgcg ggtccgcagc gagtcgccgg
      961 ttgctggctg caagcacgag tgtggccgcc atcacgccaa ggggcgcgcc aagcagcgca
     1021 tcggcgccac tgctcaaaac ccgtgctgcg atcagcgccg cctgcaacgc gagcagcacc
     1081 agaagtccga aatgaagcaa ggaccaggga gattcgcgcg tgatgctcat gtcgtcctcc
     1141 gcttcagctt gcgcgttgcg ccgcgcttct cgctcgccgg ctgcaccacg ccgagcagga
     1201 agctgaccgc ctcctccagc gccgaaatat tgagcctgta gcggatcgaa ttgccgtcac
     1261 gctcgggctc gatcaggtcg gcctccttca gagcgttgag atgtcccgtc acggaaggcc
     1321 aggccatgtc gaaggcagcg gcgatgtcgc ccgaggacat gggcccggtt tgcagcattg
     1381 aaacgatctc gcgtcggaca gggtggctta gcgccttgaa gagcgtgttc atagttttcc
     1441 gcctaattag gatacatcct aaattaaggc cgccagggtt gtcaacgggg accagtaatg
     1501 ggtccgccgg cctgacgatc gtgagagagc gccggatgca caactacggc accgcgtttt
     1561 tcccgtcact cccagtttca atcatttctc ggcttgttcc gtctggcgtt ctgcccgtcg
     1621 cgccaaatgg ataccgtcag acataccgcc aagacgcggc ccaaccgcac gtctaagccg
     1681 gggtgaggtg caacgggttg gtggcttcct cgcagacgtc gggcgcctag ctggcgtcct
     1741 gcgaactcaa tctgctctag cagcctatcg ggcttagcgc gttatttgtc tctgcccgcc
     1801 cccaggatgc gagacggcag cggtatgttc cttgcatccc gtggctatga acctatcacg
     1861 cactttttgc gctcttctga ttggacttgc aggctttgtt tgtggctgtg ccgaaacttc
     1921 aacagcgctg cagaaggcgg aggcacaacc ggagcagaaa gctgaaccgg agcagaaagc
     1981 tgacgcacaa gatcaacgga ggattctgaa tgagggctac agtatttttt acagcaccgt
     2041 cgatggtctg tccaagttcg acctgatctt tctcggcaag tctgagacgg agtcagtcta
     2101 tgaggtgaca acagcggtca ccgagtacgc cgataccctc aaggcgacgc ttgaccgcgt
     2161 ggcaaaggac tacccggccg tcaatatgga actcgatccg ttgcccgtga tcgagcaacg
     2221 cacacgcgga ggaatcacaa cagatcgcct attgagcttt gcacccgtcg taggcctcac
     2281 gggtgagagc ttcgaccgcc ggatgttgca ggtcctcgaa ggcacgctca acaacctgcg
     2341 gttcatggca aaagcgttgg cggacgaaga accagaaccg agcctgaaac aaatcgcgac
     2401 cactgcccat aacaggtttg agcagctcta tggccaggtc ctcaacatgc tcgacgagaa
     2461 gtacttcata aacaccgggg agcggaaagt cgcgaaggcg gcaccatgat gccgcggtcc
     2521 ccgttcaaaa tttggccacc gttcccgccg tccggcgagt gaaccgacgg atcaggaggc
     2581 caggttcaat gcatccgcgt tgaatagatc atcccgcagc gatggaccgc gaacgatggc
     2641 gaacgagccg tccgccttgc ggaagctaaa cgatcgggag agtaaatcgc agcgtgcttc
     2701 cgaggccttc gatgctctcg gcccaaatcc gtccgcgatg agcctcgacg atgcatcggg
     2761 tgatgtagag cccaagccct acgcctcgtc gatcatcgct ccgcacctgc cagaagcgct
     2821 ggaagatcgc ctccagcata tgggccggta ttcccgagcc tgtgtcggtc acggcgcagc
     2881 gtagctcgtc cgctccgcgc gcggcctcca cgcgtattct gccgccacgt ggagtgaact
     2941 tgagcgaatt ggcgatgagg ttagcgagca cttgcagcat gcgatcgtga tcgaaccgca
     3001 tcgggagaga tcgttctgct aacgcgattt cgagggtgat gccctgcgcc gatgccgagg
     3061 gtcggaatag cgccgccact tcctctatca atgcggacca atccgcgagc gcaggtacga
     3121 ctttgagttg ccctgcgtcg atggaggcta cgtcgattag gtcaccaatc aggcggttca
     3181 ttcgggcggc gtagcgttca atgcgccgct tcgttccatt tggctcggac gtgctatccg
     3241 gtagcagcgc cgcgctcaac acgattccgg aaagcagatc gcgcaaatcg tgagtaacta
     3301 tccccaagaa attgtcgcga tgcgcgaccg cgtcgtccgc acgagcgcgc tccgttagca
     3361 agtatcggtc cgttttttgt cgctccagcg gcagcagcgc tgcgagaatg cggacattct
     3421 cttcgcgttg ctggcgcaaa gcctcgtcgg cgtcggcgcg cgcgtcctgg agtgcgtcgt
     3481 cctccaccgc ccgctgttcg gcgagcaccg tgtgcggcag aacatgctcg tcaccgcccg
     3541 cgcgcagcaa ctcatcggcc gtaacgcgag cggtggccac aacggcgtcg gccaccgatc
     3601 gtgcttgctc gacagtctgg tccgcgttct cgtcgacggc gacctgtctg tctaccaacg
     3661 cttgatcggt cttctcccgt tcgacgcgta ggcttgcgtc cgtattttcg cgctccgggt
     3721 tgatttggct ggacatttcg atccttttga acccaggaaa ggcccgccgt tagacaacga
     3781 aatggtgcaa acgacacgac aagaatcgat tgggcggtga gccccgtggt cggactccgt
     3841 tcggccgtag cgattccgcg atctttagcg gaagaagctt gccgccgagc gcacgatgtc
     3901 cgcggccgac ggctgcgacc gtgcggtcag agccatgagc aacggtaccg tcgccaggtt
     3961 gaactcgagc accgccgttt catagccccg ctagctcgtg ggtctctggc ctcgccgatt
     4021 ttctggtttc atcgctgatg actcaagcta gtcgggctcg cagaagactg ctgtgcggtg
     4081 acacacccaa tctgtcccgg cgaagtagcg caggttcgag cggataatgg gccctcaggt
     4141 ctatgtgggg taccgcacag agcgctcaac gtggtctgta cggttttgcg tgtcttcggc
     4201 catcggtgcg tcggctgtcg ttccggctgc cggtcctggc ctagcggtac gcttcacggt
     4261 cgcaagcccg acccactcca agagccggat gtaaaaccag ccgatgtcga gttcccacca
     4321 cttgttcgaa aatttcgccg agtaggcgtg ctcatggtgg ttgttgtgca attcctcgcc
     4381 cccgatcagc acaccgaccg gaatgaggtt gcgtgattcg tcgggtgtct cgaagtttcg
     4441 gtaaccccag taatgtgcgg caccattaac cacgcctgcc gcccaaaacg gtatccacaa
     4501 catttggact gcgtaaatga tcaaccccgg taaaccgaac agaaaaatat cgaggacgag
     4561 catgagtcct attcccaaga aacggcgagc tccatacacg cgttcctcga tccagtcgtt
     4621 cggcgtgccg gttccgtacg cgcggacagt ttcaatgtcc cgcgctgcaa ttcggtataa
     4681 agagactcca ttgaaaagca cacgccaaat gccgtacacc tgcggactgt gcggatcccg
     4741 atgcgtttcg acggtggcgt ggtgcttccg atgcaccgct acccactcgc gcgtgatcat
     4801 gccggttgtt atccaaagcc aaaaccgaaa aacatgactg accacgggat gcaaggtcag
     4861 agatcggtgc gcttggtgac ggtgaagaaa aatcgtcacg ctcacgatgg tcgcgtgggt
     4921 catcacgagc gcagctgcga cgtaacccca aaaggagagc tcgatgagac cgtgggacgg
     4981 cattaggtcg tgaagcaaga cctgtgccag aaccgcgcgt ctaggccgga aacacccggt
     5041 tcttatggct cattgcgata tcacggctcg ccggacgcaa gacgattcga gcgacgaaag
     5101 agcctgcgcc gcgcaagcgg ttcgaacggc tcgtcgggac cgccggggcc gagcacgtcg
     5161 ttcttggcga gccactcttc gactgccgac aagtccggcg tctgataggg gcaaagcgtc
     5221 ttgacgtctc cgcgcagctc ttcgatcgcc gcgagaagcg acccgcgttc gcggatctgc
     5281 gcagcgatcg ctatcgcgtc cacgcccaag tgctcggaca agagcccgtt gtgaaacgcg
     5341 cgtatggccg ggccgcatga cgcgttagcc cccaatgtgg tcgaaatcaa aacgtcgcat
     5401 tcagtgtcga gccgcatcga gcggcgttca agttggagga ccctacacgc aacgcgacgt
     5461 cgtcgacgat catgactttc gcgtggacgt aaactgggt
//
LOCUS       DASUOM010000051         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1672349,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000051 DASUOM010000000
VERSION     DASUOM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672349"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFY27_01090"
     CDS             complement(<1..474)
                     /locus_tag="VFY27_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281449.1"
                     /note="has 3'-5' exonuclease, 5'-3' exonuclease and
                     5'-3'polymerase activities, primarily functions to fill
                     gaps during DNA replication and repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5764132.1"
                     /translation="MTRKTLVLVDGSSYLYRAYHALPKLSSSKGDPTGALLGVLNMIN
                     RLLKDEPTECFAVVFDAPGKTFRDVMYEQYKANRETMPDELRAQTEPLLAAVEALGLP
                     LLRIEGVEADDVIGTLCRQCAGQDLKVLVSTGDKDMAQLVNEDVTVVNTMTGSVLD"
     gene            complement(490..789)
                     /locus_tag="VFY27_01095"
     CDS             complement(490..789)
                     /locus_tag="VFY27_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX5764133.1"
                     /translation="MKQKLKLVLPVLLSLLACACGQKGPLFLPGDRSKIYTELPEMDR
                     EALEDAFEDEQEEGTRDVPDTERPQGTDDEAEPFIDPASPPSPSPPDDHDEDGIE"
     gene            complement(903..2087)
                     /gene="argH"
                     /locus_tag="VFY27_01100"
     CDS             complement(903..2087)
                     /gene="argH"
                     /locus_tag="VFY27_01100"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5764134.1"
                     /translation="MTRLWEKGLPLDERVLRYTAGEDHLLDARLVPYDVRGSIAHAEM
                     LCRQQLLDEADCEAICRGLRELEQQFTAGEWRIELGDEDVHTALESRLTVLVGKAGER
                     LHLGRSRNDQVLTALRLYLVDAADEVSASAELLCLELDRLAERQGEVILPGYTHMQQA
                     MPSSVLLWSGAFREAFQDDVAGIRSTQRRSSRNPLGSAAGYGTPGLRLDRDSTTEALG
                     FAETQVPVTAVQLSRGKAECTLMFELNLLVQDLGRLASDLLLFYTQEFGYVELPAGFT
                     TGSSIMPQKKNPDVLELVRAASATAQACLDEGLMITAKLPSGYQRDLQRLKPPLFRAI
                     DLALESTDIMRGLIAGLRFLPERIRLSEGLDAAEEAYNLVTREGIPFREAYRRVASRY
                     KD"
     gene            complement(2084..3022)
                     /gene="argB"
                     /locus_tag="VFY27_01105"
     CDS             complement(2084..3022)
                     /gene="argB"
                     /locus_tag="VFY27_01105"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5764135.1"
                     /translation="MKQRHDRAVVVSALKHAAPYIRLYKGRVFVLKVGGEVFADLGST
                     RALMEQVGILNQVGIRVVLVHGGGPQSTRLAAALGLDATFIEGRRVTDGASLDVATMV
                     LNGQINTRVLASCRDLGIPAVGISGVDAGLIRAFRRPPVQREGEQPIDYGFVGDIESV
                     DADVLRKQLDNALMPVVSPLSCDSSGNLLNINADTVAAAIAAELKAEKLILATGAPGI
                     LDNAADPTSLISYIDRAGLKKLTQSGKLADGMLPKAAAIDAALINGVQRVHVISYKVP
                     DSLLLEVFTNEGTGTLVVNDIAALSPAEQASAGTDA"
     gene            complement(3019..>3729)
                     /locus_tag="VFY27_01110"
     CDS             complement(3019..>3729)
                     /locus_tag="VFY27_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764136.1"
                     /translation="IASYGDAIGIRAFAERRSLEADLADAEFQSMIQLIETPYINMES
                     AINHPCQSLADWKTLDELDIPANGGKFVLSWAYHPLALPLAVPASTLHMAAMRGMDVT
                     VLRPEGFALPESLMQKARAAADASGGTVRETEDRKEAMEGAKVIYAKSWSSTAAYGNK
                     VKDLELRRGLEHWCVDEPWFENAAPDCRFMHCLPVRRGVVVTEHVLDGPRSVVIQEAR
                     NRMLAQMAVLHQMLGSKS"
BASE COUNT          627 a         1192 c         1184 g          726 t
ORIGIN      
        1 gtcgagcacg ctgccggtca tggtgttcac gaccgtcacg tcttcgttga cgagctgcgc
       61 catgtccttg tctccggtgg agacgagcac cttcaggtcc tgtccggcgc actgtcggca
      121 cagcgttcct atcacgtcgt ccgcttctac gccttcgatc cttagcagcg gaagcccgag
      181 cgcctccacg gcggcaagaa gcggttcggt ttgcgcgcgt agttcgtcgg gcatcgtctc
      241 gcggttcgcc ttgtactgct cgtacatgac gtcgcgaaag gttttgcccg gcgcgtcgaa
      301 cactaccgcg aagcactcgg ttggctcgtc cttcagcagc cggttgatca tgttcagcac
      361 gccgagcagc gcgccggtgg ggtcgccttt cgaggaagaa agcttcggca gggcgtgata
      421 ggcgcggtaa aggtaggaag agccatctac caggacgaga gtcttccggg tcaccgggcg
      481 ggccttccgt tattcaatgc catcttcgtc gtgatcatcg ggcggcgagg gcgagggcgg
      541 ggaagcgggg tcgatgaatg gctcggcctc atcgtcggtg ccctgcgggc gttccgtatc
      601 cggtacatcc cgggtcccct cttcctgctc gtcctcgaag gcgtcttcca gcgcttcgcg
      661 gtccatttcc ggaagctccg tataaatttt gcttcggtcg ccaggcagga aaagcgggcc
      721 cttctggccg caggcgcagg cgaggaggct tagcaggacc ggaagtacga gtttgagctt
      781 ctgcttcatc tacagggatc gggccgcggg tggcgcagtc aaaggagtcg aatggtataa
      841 cagttggacc tgatcggctt ccggccggaa ttcccgaagc cgcgcccgaa aactcgagcg
      901 gatcaatctt tgtaacggga ggcgaccctc cggtatgcct cgcggaacgg gattccctcg
      961 cgggtcacga gattgtatgc ctcttccgcc gcgtcaagac cctcgctcaa acgaatgcgc
     1021 tcaggtaaga accttaagcc cgcgatgagt ccgcgcatga tgtcggtgct ctcgagggcc
     1081 aggtcgattg cgcgaaagag aggcggcttc agtcgctgca ggtcgcgctg gtagcccgac
     1141 ggtagtttcg ccgtgatcat gagcccctcg tcgaggcagg cctgcgcagt tgcggaagcg
     1201 gcacgcacca gttccagcac gtccgggttt tttttctgcg gcatgatcga tgagccggtt
     1261 gtgaacccgg ccggtaactc tacgtaaccg aactcctgcg tataaaaaag gagcaggtcg
     1321 ctcgccagcc tgcccaggtc ctgcaccagc aggttcagtt cgaacatcag ggtgcactcg
     1381 gccttgccgc gggaaagctg gactgccgtt accggtacct gggtttcggc aaacccgagc
     1441 gcttcggtgg tcgagtcgcg atcgagccgg agccccgggg tgccgtaccc ggcggcgctt
     1501 cccagggggt tgcggctcga gcggcgctgc gtgctgcgaa tgccggccac gtcgtcctgg
     1561 aaagcttccc tgaacgcgcc actccacagg agcaccgaag acggcatggc ctgctgcatg
     1621 tgcgtgtatc ccggcaggat cacctcaccc tggcgttcag caagcctgtc cagctcgagg
     1681 cacagcagtt ccgcactggc agacacttcg tcagccgcgt caacgaggta gagtcggagc
     1741 gccgtcagta cctggtcgtt gcgtgagcgg cccaggtgca ggcgctcgcc ggctttgccg
     1801 accagcaccg tcagccgcga ctccagcgcc gtgtgcacgt cttcgtcgcc gagttcgatc
     1861 cgccattcac cggctgtgaa ctgttgctcg agctcccgca agccgcggca gatcgcttcg
     1921 cagtccgcct catccagcag ctgttgccga cacagcatct cagcatgcgc gatggagccg
     1981 cgcacgtcgt agggcaccag ccgtgcgtca agcaggtgat cttcgcccgc cgtatagcgc
     2041 aacacgcgtt cgtccagggg gagtcccttc tcccacagcc tcgtcatgcg tcggttcccg
     2101 cagacgcctg ttcggccgga gacagcgcgg caatgtcgtt cacgaccagc gttccagtac
     2161 cctcgttggt gaatacctcc agcagcaggc tgtccggcac cttgtaggag atcacgtgca
     2221 cgcgctgcac gccgttgatg agcgccgcat cgatggccgc tgccttcggc agcatgccgt
     2281 cggcgagctt gccggattgc gtgagcttct tgaggccggc cctgtcgatg taggaaataa
     2341 gagaggtcgg atcggctgcg ttgtcgagga tgcctggcgc gccggtcgcg aggatcagct
     2401 tttccgcctt caactcggca gcgatcgccg cggcaaccgt gtctgcattg atgttcagca
     2461 ggttcccgga cgagtcgcag gaaagcgggc tgacgaccgg catcagcgcg ttgtcgagct
     2521 gcttgcgcag cacgtccgcg tcgaccgact cgatatctcc gacgaagcca tagtcgatcg
     2581 gctgctcgcc ctctcgctgc accggcgggc ggcggaaggc gcgaatcagg ccggcatcga
     2641 ctccgctgat gccgacagcg ggtatgccga gatcgcggca ggaggcgagg acgcgggtgt
     2701 tgatctggcc gttcaggacc atcgtcgcga catcgagcga tgcgccgtcg gtgacgcggc
     2761 gcccttcgat gaaggttgca tcgaggccga gcgccgcggc gagccgggtg gattgcggtc
     2821 cgcctccgtg cacgagcacc acccgtatgc cgacctggtt caggatgcct acctgttcca
     2881 tgagggcgcg ggtgctgcca aggtcggcga atacttcgcc gcccaccttg agcacgaaaa
     2941 ccctgccttt gtacaggcgg atgtacggcg ccgcgtgctt cagtgcggag acgacaacgg
     3001 cccggtcgtg ccgctgcttc acgatttgct gcccagcatc tggtgcagta ccgccatctg
     3061 cgccagcatc cggttgcgcg cttcctggat caccacgctg cgaggcccgt cgaggacatg
     3121 ctcagtcacg accacgccgc ggcgtaccgg caggcaatgc atgaagcggc agtcgggcgc
     3181 cgcattttcg aaccagggct catccacgca ccagtgctcc aggccgcgtc tcagctccag
     3241 gtccttgacc ttgttgccgt acgccgccgt cgaggaccac gatttcgcgt agatgacttt
     3301 cgcgccttcc atcgcttcct tcctgtcttc tgtttcccgg acagtaccgc ccgatgcatc
     3361 ggccgccgcg cgggcctttt gcatcagcga ctccggcagc gcgaaacctt ccggccgcaa
     3421 tacagtcacg tccatgcccc gcattgcggc catgtgaagc gtcgacgcag gtacagccag
     3481 cggcaaggcc agcgggtgat aggcccagct caggacgaac ttgccgccgt tcgccgggat
     3541 gtcgagttcg tccagcgtct tccagtcggc gaggctctgg cagggatgat tgatcgccga
     3601 ctccatgttt atgtacggag tttcgatcag ttgaatcatc gactggaact ccgcgtccgc
     3661 aagatcggct tcgaggcttc tacgttccgc gaaggcgcgt atgcctatag cgtcgccata
     3721 ggacgcgat
//
LOCUS       DASUOM010000052        10574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_705351,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000052 DASUOM010000000
VERSION     DASUOM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705351"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VFY27_01115"
     CDS             <1..1658
                     /locus_tag="VFY27_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5764137.1"
                     /translation="TDDTYDLVVVGGGLSGLSAAFLYRQRTGRDARILVLDNHDDFGG
                     HAKRNEFDVGGRKLICYGGSQSIDGPGNYSAAAKKLLRDISVHTERFYDYFDQEFYRE
                     NELGRAMWFSREAYGEDRLLPNVMRGWDGDAASDIAATVDRYPLQEAAQQALIDLLTS
                     ERDLLPDLSREQKIALLRSTSYSGFLQAHAHMPPEVPELFRDSIKGLWGVGWDALSAL
                     EAYRSGMPGTAGLEIGELENDPPGREEPYIFHFPDGNAGVARALVRRLIPEAVPGETM
                     EDLVSAKVRYELLDQEGANSRIRLNSTAVDVRHAEDGKAVDIAYVRSGETYRVRAKHA
                     VLACYNNIVPHICSEVPQEQREAIEYATKVPLVYISIAIRNWQAFDKLGYHSIYVPKA
                     DLMHSFGLDFPVSMGDYRFPSSPEQPAVIHGGFVPTLPDQGLSAREQHVAGRRRLFEM
                     SFEDFEKGVLRQLDGALSEGGFDAERDIAAITVNRWPHGYAYEYNDFSDPPDWGRDKG
                     PHIAGRAQIGRISIANSDAAAYAYVDGAIDAAHRAVEEQLAHG"
     gene            1764..3125
                     /locus_tag="VFY27_01120"
     CDS             1764..3125
                     /locus_tag="VFY27_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX5764138.1"
                     /translation="MEPDFSSVIPTLVVFVLAILTRRPIESLVTGAVVGLAMIHGSGF
                     VTGFAETSLRVMTDEDVAWVILVCGFMGGLIAVLMRTGATSAFTAIMTRYVRSEKSAL
                     FATWVLGIFMFVDDYLNSLAVGAAMRDVTDKYKVSREKLAYVVDSTAAPVSVVIPFST
                     WGAFFGGLLVANGVAAEGEGLTTYIAAIPYMLYAWIAVFLVPVVIAGWVPSLGGMKAA
                     EVRAKTKNQMVPPDALHIEQANKAIMPHPAVKPRVSMFVLPMFALVFFTLYFDKDFLT
                     GIYITLGGTALVILGLRILDLNDTFDAIIDGFKTMIEPLGVLVAAFILKDVNDALQLP
                     TYVVTTMEPVLTAESLPAVIFASMGLVSFMTGSNWGVFVIILPIVVSLGQNLGTDMAL
                     VIGATLSASTFGSHACFYSDATVLTAQATGCTAMQHALTQIPYALIAAAIAIVGYLIL
                     GYV"
     gene            3163..3624
                     /locus_tag="VFY27_01125"
     CDS             3163..3624
                     /locus_tag="VFY27_01125"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764139.1"
                     /translation="MKKLLLPVALLVAGMSLGAVLAAHHGPEHDAVLPAKISKEDMTG
                     KIFERPDTITRTSGGNTIKDVTSLKSSDKKFASGMYKSGKVRFEATEPYGVDEFMYFL
                     EGGVKLTSADGSVQVINAGEAVTIPKEWTGVWDTDGYTKIWVIYSEDGSGL"
     gene            3732..4649
                     /locus_tag="VFY27_01130"
     CDS             3732..4649
                     /locus_tag="VFY27_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764140.1"
                     /translation="MLLIRLVIAVFALPAAALAQVTPANLPGETEWYLHADLDELRNA
                     SSGKHIYQWLEGEVFIDIRDDLGIDLGKETSSLTAFSDASLGTVIVVNGAIADSTRDK
                     LLAMAATDSKLQTLEHDGKTYYHAERSKGRSGNGRSFDDLEKSAYFSFDVRNKLLIAS
                     DPNQIKALLESGGRMAGNQDHRGALLVLTADKSFLQAGARTAKFADDDDESWNSNILR
                     NTELVALLVSDRDGLIAVEAQLVSHEPTMAQSLAGIINGLISLQALNPGLDPQVVSLI
                     QNTRVDVKDKILSINAVINPQMVVDLLND"
     gene            4656..5219
                     /locus_tag="VFY27_01135"
     CDS             4656..5219
                     /locus_tag="VFY27_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5764141.1"
                     /translation="MPTDAELVHQAKRGSASAFERLVERYQQRLFRFLVTRCATRADA
                     EDALQDTFVNAFRYLDTYNPHWRFSTWLYRIAMRNALNMASGNSVLAADVDTEVADDA
                     PGLLQECIRESERENLWLTAKRLLSDEVFTAMWLRYAEDMPVREISRSLGRSQAWTRV
                     SLLRARKRLAAGMTGDGATGKESKAYG"
     gene            5212..5616
                     /locus_tag="VFY27_01140"
     CDS             5212..5616
                     /locus_tag="VFY27_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764142.1"
                     /translation="MDELERRLREDAHEIRAEVPAGLRARIGESLRSARPMPPARRDF
                     SWPLWLAASVTGAAAAVVAIVSVNVATEAPEATDSPAYSVPQYVNQLHLEIPLRATSA
                     DLTAPLEEEMKNLRSDLERARENVEQDLEFTF"
     gene            6043..7473
                     /locus_tag="VFY27_01145"
     CDS             6043..7473
                     /locus_tag="VFY27_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5764143.1"
                     /translation="MPLAIVLILLVVGSIVFHFVSPWTFTPLASNWSEIDDTIGITFW
                     VTGFVFVAVNLFMAYAIIRYRHKKDQRANYEPENKKLEGWLTVFTTIGVAAMLAPGLF
                     VWASFVEVPEDAMEVEAVGQQWHWSYRLPGEDGEFGESDLKLVTDDNPFGMDVEDPAG
                     QDDILVSDPQLHLPVDRPVKLWLRSKDVLHNFTVAQFRVKMDLVPGMVTYMWLTPTRT
                     GRYEVLCEELCGIAHHTMRGAVMVEEQQDFDEWAGSYPTFAELQQPAQAVAAAGAAQY
                     VVCAACHGQQGEGMQALNAPKLAGQDPWYLKRQLRNFKNGLRGTHEEDVFGKQMAPMA
                     MTLATDQAIDNMVAHIQSFPDTSPTHTVEGNVAAGAERFTVCAYCHGANGEGIQAMNA
                     PRLAGISDWYLEHQIRNFKRGIRGEHPEDYYGKQMGFMGRIFQTDQSINDVVAYINTL
                     DAPAERVAASAAVPDDQPTGQTSSQY"
     gene            7498..9261
                     /locus_tag="VFY27_01150"
     CDS             7498..9261
                     /locus_tag="VFY27_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5764144.1"
                     /translation="MAYVAIADRDHEIEHHDPQTFITKYVWSQDHKVIAIQYGLTAIF
                     VGVIALCLSVMMRLQLGFPDNFSFITPESYYQFVTMHGMIMVIYLLTALFLGGFGNYL
                     IPLMLGSRDMVFPYMNMLSYWVYLLSVIILLTSFFVPGGATGAGWTLYPPQAIARGTP
                     GVDWGIVLMLVSLAVFIVAFTMGGLNYVTTILQARCRGMTLMRMPLSVWGIFMATVLG
                     LLAFPALLVSAIMMLLDKTLMTSFFMPAVVSAGVQTGHESGSPILFQHLFWFFGHPEV
                     YIVALPAFGIVSDLLSTHARKNIFGYRMMVWAILAIGGLSFIVWAHHMYVSGMNPYFG
                     FFFATTTLIIAVPTALKVYNWVLTLWQGNIHLRVPMLFAIGFIFTFIHGGLTGIFLGN
                     VVVDLPLSDTYFVVAHFHMVMGVSPILVVFGAIYHWFPKMTGKMYNETLGRWHFWLTF
                     LGTYSIYLPMHYLGFLGVPRRYFAMGNTDFMPESAHALNTSITISAIFVALTQLIFFY
                     NMITSLRKGEKAGPNPWGATTLEWQTADTPPKHGNWGHDLPEVHRWAYDYSVPGYPED
                     FIPQNVPAELAPVGRDHGKQR"
     gene            9283..9945
                     /locus_tag="VFY27_01155"
     CDS             9283..9945
                     /locus_tag="VFY27_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764145.1"
                     /translation="MTVSLVLLAIIMFVVIGWLLRQTLNTQPWLPRAADNAASGTSID
                     TPAKTFGLVSFLAVATSLFALFISAYAMRMHMGDWRPVSEPQLLWVNSAFLVFASIAY
                     ELTRRAAGRGRAGALRPGLVIAGLFTFLFLGGQLVAWLQLNASGHYMTSNPASAFFYV
                     LTAVHGLHLLGGLWVWARSTVKVFSGAEADSVRLSVELCAVYWHYLLLVWAVLFGLLL
                     AT"
     gene            10007..>10574
                     /locus_tag="VFY27_01160"
     CDS             10007..>10574
                     /locus_tag="VFY27_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5764146.1"
                     /translation="MSEAALAKPGDVLPAGTRGVVTDFSSDKQAFHVPWGKAMMWIFL
                     LSDTFIFSCFLVSYMTVRMSAAEPWPYPSEVFALSFGGDPIPLVLIAIMTFVLITSSG
                     TMAMAVNMGYRKDRTKTFWLMVATAVLGATFVSMQAFEWTKLIVDEGVRPWGNPMGAA
                     QFGSSFFMITGFHGMHVSAGVIYLLVVAF"
BASE COUNT         2015 a         3273 c         3220 g         2066 t
ORIGIN      
        1 agaccgacga cacgtacgac ctcgtcgttg ttggcggcgg cctgtccggt ttgtccgccg
       61 cattcctcta tcgccagcgc accggtcgcg atgcgcgcat tctcgtgctc gacaaccacg
      121 acgacttcgg gggtcatgcg aagcgcaacg agttcgatgt cggcggccgc aaactgatct
      181 gttacggcgg cagccagtcg atcgacgggc ccggtaacta ctcggcagcg gcaaaaaaac
      241 tgctcaggga catctcggtt cacaccgaac gcttctacga ttacttcgac caggaatttt
      301 accgggagaa cgagctcggc cgtgcaatgt ggttcagcag ggaagcctac ggcgaggacc
      361 ggctgctgcc gaacgtcatg cgtggctggg acggcgacgc tgcgagcgat atcgccgcga
      421 ctgtggaccg ttatcccctc caggaagccg cgcagcaggc cttgattgac ctgttgacga
      481 gcgagcgtga cctgctgccg gatctctccc gcgaacagaa gatcgccttg ctgcgaagca
      541 ccagctattc cgggttcctg caggcgcacg cgcatatgcc gccggaggtg ccagagctgt
      601 tccgcgattc gatcaagggc ttgtggggcg tcggctggga tgcgctctcg gccctggagg
      661 cataccggag cgggatgccg ggcacggcag gcctcgaaat cggcgagctg gagaacgacc
      721 cgcccgggcg tgaagagccc tacatcttcc actttccgga cggcaacgcg ggcgtcgcca
      781 gggcgctcgt ccgccggttg atcccggaag ccgtgcccgg cgagaccatg gaagacctgg
      841 tctctgcgaa agtgcgttac gagctgctcg accaggaggg cgcgaactcc aggatccgct
      901 tgaacagcac ggcagtggat gtgcgccatg cggaggacgg caaggccgtc gacatagcct
      961 atgtccggag cggggaaact tatcgcgtgc gggcgaagca cgccgtcctg gcctgctaca
     1021 acaacatcgt cccgcacatc tgcagcgaag tcccgcagga gcagcgcgaa gccatcgaat
     1081 acgcgacgaa ggttcccctc gtatatataa gtatcgcgat acgaaactgg caggcgttcg
     1141 acaagctcgg gtaccacagc atctacgtac ccaaagcgga cctcatgcat tctttcggtc
     1201 tcgatttccc ggtcagcatg ggcgactaca ggtttccgtc gagtccggaa cagccggcgg
     1261 tcattcacgg cggcttcgtt cccacgctcc cggaccaggg actttccgcg cgcgagcagc
     1321 acgtggcagg ccggcgcagg ctgttcgaga tgagcttcga ggatttcgag aagggtgtcc
     1381 tgcggcagct cgacggtgca ctctcggaag gcggcttcga tgccgaacgc gatatcgcag
     1441 ccattaccgt gaaccgctgg cctcacggtt acgcgtacga atataacgac ttttccgacc
     1501 ctcccgactg gggacgcgac aagggcccgc acattgccgg gcgcgcgcag atcggccgca
     1561 tctcgatcgc gaactcggat gccgcagctt acgcctacgt ggacggcgcc atcgacgccg
     1621 cccaccgcgc ggtagaagaa caactcgcgc atggctagcg cggcggggca aaatctggca
     1681 cctgtaggag cccatgcggg cgattgcgat atgcaccggc gaactcgccc gcacggcggg
     1741 ctcctgcagc gccaggaata aaagtggaac cggatttcag ctccgtcata ccgacgctcg
     1801 tcgtcttcgt gctggcaatt ctcacgcgcc ggcccatcga gtcgctcgtg accggcgcgg
     1861 tggtcgggct cgccatgatc cacggatcgg gattcgtcac gggttttgcc gagacttcgc
     1921 ttcgcgtgat gaccgacgag gacgtagcct gggtgatcct cgtctgcggt ttcatgggcg
     1981 ggctgatcgc ggtactgatg cgcacgggtg ccaccagcgc gttcacggcc atcatgaccc
     2041 gctacgtacg ctccgagaaa agcgcgttgt tcgcaacctg ggtcctcggg atattcatgt
     2101 tcgtcgacga ctatctcaac tcgctcgcgg tcggcgcggc gatgcgcgat gtgaccgaca
     2161 agtacaaggt gtcgcgggaa aagcttgcgt acgtcgtcga ttccactgct gctccggtca
     2221 gcgtcgtcat cccgttttcc acctggggcg ctttctttgg cggactgctg gtggcaaatg
     2281 gtgtcgcagc ggagggcgaa ggccttacca cctacatcgc tgctatacct tacatgctgt
     2341 acgcctggat agccgtcttc ctggtgcctg tcgtgatcgc gggttgggtt ccttccctcg
     2401 gcggaatgaa ggcggccgaa gtccgcgcga agacgaagaa ccagatggtg ccccccgatg
     2461 ccctgcacat cgagcaggcg aacaaggcca tcatgcctca ccccgcggtc aagccgcgcg
     2521 tgtccatgtt cgtgctgccg atgttcgcgc tcgtgttttt taccctgtac ttcgacaagg
     2581 acttcctcac cggcatctac ataacgcttg gcggcaccgc gctggtgatc ctgggcctcc
     2641 gtatactcga cctgaacgac acgttcgatg cgatcatcga cggcttcaag accatgatcg
     2701 aaccgctcgg ggttctcgtg gccgcgttta tcctgaagga tgtcaacgac gcgctgcagc
     2761 tgccgactta cgtcgtaacc acgatggagc cggtactgac ggcggaaagc ctgccggcgg
     2821 tgatttttgc atccatgggg ctcgtctcgt tcatgaccgg gtcgaactgg ggtgtcttcg
     2881 tcatcatcct gccgatagtc gtatccctag ggcagaacct cggaacggat atggcgctgg
     2941 tcatcggtgc aactctttcc gccagcacct tcggcagcca cgcctgcttc tactccgacg
     3001 cgacggtgct cacggcgcag gccacgggtt gcacggccat gcagcatgct cttacgcaga
     3061 tcccgtacgc cctgattgcc gccgcgatcg ccattgtcgg ctacctcata ctaggctacg
     3121 tatgatgaaa cattcttttg gcttaccagg gaggaaaacc cagtgaagaa attgctgctg
     3181 cccgtcgcct tgcttgtggc tggaatgagc ctcggcgccg tcctcgccgc ccaccacggc
     3241 ccggaacacg atgccgttct cccggcaaaa atcagcaagg aagatatgac gggcaaaatt
     3301 ttcgagcggc cggatacgat tacccgtacc agcggcggca acacgatcaa ggatgtcacc
     3361 tcgctcaaga gcagcgacaa gaagtttgcc tccggcatgt acaagtccgg caaggtccgc
     3421 ttcgaggcaa cggagccgta cggcgtcgac gagttcatgt acttcctcga aggcggcgtc
     3481 aagctgacct ccgcggacgg ctcggtacag gtcatcaacg ccggcgaagc cgtcactatc
     3541 ccaaaggaat ggacgggtgt atgggacacc gacggctaca cgaagatctg ggtgatctac
     3601 tccgaagacg gctcgggcct ctagactcgc cacaaagggg acacggtccc cttttcctcc
     3661 cgggtgtaac gttttccttc gccgggcgct ataagaggta acaacctcgc cctcgctcca
     3721 aggaaacgaa catgctcctg atacgcctcg tcattgccgt tttcgcgctc ccggccgccg
     3781 ccctggcgca ggtgacgcct gccaaccttc cgggagagac ggagtggtac ctgcacgccg
     3841 acctcgacga gctgcgcaat gcgagctccg gcaagcacat ctaccagtgg ctggaagggg
     3901 aggttttcat cgacattcgc gacgacctgg gcatcgatct tggcaaggag acaagttcac
     3961 taacagcgtt ttccgatgca tccctgggga cggtcatcgt cgtgaatggg gctattgcgg
     4021 attcgacgcg cgacaagctg cttgcgatgg cggcgaccga ttcaaagctc cagaccctgg
     4081 agcacgatgg caagacctac taccatgcgg agcgcagcaa agggcgatcg ggaaacggcc
     4141 gctcgttcga tgacctggaa aagtccgcgt acttcagctt cgacgtcagg aacaaactcc
     4201 tgatcgcttc ggatccgaac cagatcaagg cgctgctcga gagcggcggc cgcatggccg
     4261 gtaaccagga tcatcgcggc gcgctgctgg tgctgacggc ggacaagagc ttcctgcagg
     4321 cgggcgcgcg caccgcgaag ttcgccgatg acgacgacga aagctggaac tccaacatcc
     4381 tgcgcaacac cgagctggtg gcgttgctgg tgtcggaccg ggacgggctg atcgctgtcg
     4441 aggcgcagct ggtttcgcac gagcccacca tggcgcagtc gctcgccggt atcatcaacg
     4501 ggctgatcag cctgcaggca ctgaaccccg gcctcgaccc gcaggttgtt tccctcatcc
     4561 agaacacccg ggtagacgtc aaggacaaga ttctttccat aaacgctgtc atcaatccac
     4621 agatggttgt cgatttgctc aacgattaga cggcagtgcc cactgatgcc gaacttgtcc
     4681 accaggcgaa gagaggctct gcctccgcgt ttgaacggct ggtggagcga taccagcagc
     4741 ggctgttccg cttcctggtc acgcgctgcg ccacgcgcgc agatgcggag gatgcgctgc
     4801 aggacacgtt cgtcaacgct tttcgatacc tggataccta taacccgcac tggcgattca
     4861 gcacctggct ctaccggatc gcaatgcgca acgcattgaa catggcatcc gggaactccg
     4921 tgcttgcggc ggatgtcgac acagaggtcg cggatgatgc ccccggactc ctccaggagt
     4981 gcatacgcga gtcagagcgg gagaacctgt ggcttacggc gaagcggttg ctgtcggacg
     5041 aagtctttac ggcgatgtgg cttcgctatg cggaggatat gccggtgagg gaaatctcga
     5101 gatcgctcgg tcggtcgcag gcatggacca gggtgtccct cctgcgcgcg cgcaagcggc
     5161 ttgccgccgg gatgaccggc gatggtgcca ccggaaaaga gagtaaggca tatggatgaa
     5221 ctcgaacgac ggctgcggga ggacgctcat gaaatccgcg ccgaggtgcc tgccgggctg
     5281 cgtgcgcgca ttggcgaatc gctgcgaagc gcgcgaccga tgccgccggc gcggcgcgat
     5341 ttcagctggc cgctgtggct ggccgcgagc gttaccggcg ctgcggcagc agtggtggca
     5401 atcgtgtcgg tgaacgtcgc caccgaggct ccggaggcca cggacagccc cgcgtattcc
     5461 gtaccgcaat acgtaaacca actgcatctc gagattcccc tgcgggcaac ttccgccgat
     5521 ctcaccgccc ccctcgagga agaaatgaaa aaccttcgtt cggatctcga gcgcgcccgc
     5581 gaaaacgtcg aacaggacct cgaattcact ttctagtaaa gggggacaga tttatttttt
     5641 cctgtccctt gtaggtcgac aaaaaataaa gctgtccctt gctggtcgtc ccggacgccc
     5701 gatttttatt cttttacaat caggtttttg cgcgggacgc ttcacatgtt gaaatgcggt
     5761 cacatagtgg aaatcggcac ctgaatccac tatcctcggg cgtatcagtt cgccggtaga
     5821 atgaagcgta cggcggccac ggaagacggg gacggggagt agaaggggga ctcgcatcag
     5881 gcgaaaacct gcatgtgtca atctctcacg acgacgacag cacattcaaa accgcgagcc
     5941 gctcaaaaag cagatggcga agcttcgagg atccggcgca gatccggaag cttgccgccg
     6001 tcaaaataat ccgcgaacgt caagatagaa ggatgcgaca gtatgccttt agcgattgtg
     6061 ttgatcctgt tggtggtggg atccatcgta tttcacttcg tgagtccctg gacgttcact
     6121 ccgctcgcct ccaactggag cgagatagac gacactatcg gcataacttt ctgggtaacc
     6181 ggcttcgtct tcgtcgccgt caacctgttc atggcatacg ccatcatccg ctaccggcac
     6241 aagaaggacc agcgcgccaa ctacgagccg gaaaacaaga agctcgaggg ctggctgacg
     6301 gtgttcacaa ccatcggcgt ggccgccatg ctcgcgcccg ggctcttcgt ctgggcaagt
     6361 ttcgtcgagg ttccggagga cgccatggaa gtcgaagccg tcgggcagca gtggcactgg
     6421 agctaccgcc tgcctggtga agacggcgag ttcggcgaga gcgatctcaa gctggtcact
     6481 gacgacaacc cgttcggcat ggacgttgaa gatcccgccg ggcaggacga catcctggtt
     6541 tcagacccgc aactgcacct tccggtggac cggccggtca agctgtggct gcgctcgaag
     6601 gacgtgctgc acaatttcac cgtcgcacag ttccgggtga agatggacct ggtgccgggc
     6661 atggtcacct acatgtggct gacgccaacc cgtacggggc gttacgaagt cctctgcgaa
     6721 gaactctgcg gcattgcgca ccacaccatg cgcggcgccg tcatggtcga ggaacagcag
     6781 gacttcgacg aatgggccgg ctcgtacccg accttcgccg aactgcagca gccggcacag
     6841 gcagtggccg cggcgggcgc tgctcagtac gtggtatgcg cggcctgtca cggccagcag
     6901 ggagaaggaa tgcaggcctt gaacgcgccc aagctcgccg gccaggaccc ctggtacctc
     6961 aagcgccagt tacggaactt caagaacggc ctccgtggca cccacgaaga ggatgtcttc
     7021 ggcaagcaga tggcgccgat ggcaatgacc ctggccaccg accaggccat cgacaacatg
     7081 gtcgcccaca tccagtcctt ccccgacacg tcaccgacgc acacggtcga aggtaacgtt
     7141 gcggcgggag ccgagcgctt caccgtttgc gcgtactgcc atggtgcgaa cggcgagggc
     7201 atacaggcga tgaacgcgcc gcgactggcg ggcatttccg actggtacct cgagcaccag
     7261 atcaggaact tcaagcgcgg catccgcggc gagcacccgg aagactatta cgggaagcag
     7321 atggggttca tgggcaggat tttccagacg gaccagtcga tcaacgacgt cgtcgcctac
     7381 atcaacaccc tggacgcgcc agccgaacgc gtggccgctt ccgcggctgt gcccgacgac
     7441 cagccgacgg gccagaccag cagccagtat tagagcattc gttccgaggg gaacctgatg
     7501 gcatacgtag caatcgccga tcgtgatcac gaaatagagc accacgatcc gcagacgttc
     7561 ataaccaagt acgtatggag ccaggaccac aaggtcatcg ccatccagta cggactgacc
     7621 gcaatctttg tcggagtcat tgcgctctgt ctctcggtga tgatgcggct gcagctcggc
     7681 ttccccgaca acttctcctt catcaccccc gagagctact accagttcgt cacgatgcac
     7741 gggatgatca tggtgatcta cctgctgacc gcgctgtttc tgggcggctt cggtaactac
     7801 ctgatcccct tgatgctcgg ctcaagggac atggtgttcc cgtacatgaa catgctgagc
     7861 tactgggtct acctgctctc ggtcatcatc ctgctgacga gctttttcgt cccgggcggc
     7921 gcgacgggcg cgggctggac cctgtacccg ccgcaggcca tcgcgcgcgg cacgccaggc
     7981 gtcgactggg gcatcgttct catgctcgtc tcgctcgccg tgttcatcgt tgcattcacg
     8041 atgggcgggc tgaattacgt cacgaccata ctgcaggctc gctgccgcgg catgacgctg
     8101 atgcgcatgc ccttgtcggt gtggggaatc ttcatggcga cagttctcgg cctgctcgca
     8161 tttcccgcgt tgctggtcag cgcgatcatg atgctcctcg acaagaccct gatgaccagc
     8221 ttcttcatgc ctgcggtggt gtcggccggc gtgcagactg gccacgaaag cggcagcccg
     8281 atcctcttcc agcacctgtt ctggttcttc ggtcacccgg aggtctacat agtcgcgctg
     8341 cctgcgttcg gcatcgtgtc ggacctcctg tcgacgcatg cgcgcaagaa cattttcggc
     8401 taccgcatga tggtgtgggc gatcctcgcg atcgggggcc tgagcttcat cgtgtgggct
     8461 caccacatgt acgtcagcgg catgaacccg tacttcggtt tcttcttcgc cacgacgacg
     8521 ctgatcatcg cggtgccgac ggcgctcaag gtgtacaact gggtgctgac gctgtggcag
     8581 ggcaacatcc acctgcgggt accgatgctg tttgcgatcg gcttcatttt caccttcatt
     8641 cacgggggcc tcaccggcat cttcctgggc aacgtggtgg tcgacctgcc gctctccgat
     8701 acctatttcg tggtcgcgca cttccacatg gtcatgggcg tgtcgcccat cctcgtcgta
     8761 ttcggcgcga tctatcactg gttcccgaag atgaccggga agatgtacaa cgagacgctc
     8821 ggccgctggc acttctggct gaccttctta ggcacgtact cgatctacct gccgatgcac
     8881 tatctgggct tcctcggcgt gccgcgccgt tacttcgcga tgggcaacac cgacttcatg
     8941 ccggagtccg cgcacgcgct gaacacgagc atcacgatct ccgcgatttt cgtcgccctg
     9001 acgcagctca ttttcttcta caacatgatc acgagcctgc gcaaaggcga gaaggcgggc
     9061 ccgaatccat ggggcgccac cacgctcgag tggcaaacgg ccgacacgcc gccgaagcat
     9121 ggcaactggg ggcacgacct gccggaagtc catcgctggg cctacgacta cagcgtcccc
     9181 gggtatccgg aggacttcat tccgcaaaac gttcctgccg aactcgcccc ggtcggtcgc
     9241 gaccacggca aacagcgcta gtgctggatt ggaactgacg gaatgacggt atcgctcgta
     9301 ttgctcgcca tcatcatgtt cgtcgtgatc ggctggctgt tgcggcagac gctcaacact
     9361 caaccttggc tgccgcgcgc ggcggacaat gccgcttccg gaacctcgat cgatacgccc
     9421 gcgaaaacct tcggcctcgt cagtttcctt gccgtagcga cctcactgtt cgcgcttttc
     9481 atcagcgcgt acgcgatgcg catgcacatg ggcgactggc ggccggtcag cgaaccgcag
     9541 ctcctgtggg tgaacagcgc gttcctggtc ttcgcgagca tcgcttacga gttgacccgg
     9601 agagcggcgg gcaggggacg ggccggagcg ctcaggcccg gcctcgtcat cgcaggcctt
     9661 ttcacgttcc tgttcctggg cggccagctg gtcgcatggc tgcagctgaa cgcctccggt
     9721 cattacatga cgagcaatcc ggccagtgcg ttcttttacg tgctgacggc ggtacatggc
     9781 ctgcacctgc tgggcgggct gtgggtctgg gcgcgctcga cggtcaaagt gttttccggc
     9841 gcggaagcgg acagcgtccg gctgagcgtc gagttgtgcg cggtctactg gcactacctg
     9901 ctgcttgtgt gggcggtgtt gttcggcctg ctgctggcga cctgaatgtt tgtcacaacg
     9961 gccggtaaca ggtgctaaca gggcttacct tcgaggaaat gaactgatgt cagaagctgc
    10021 actggcgaag cccggcgacg ttcttccggc cggcacccgg ggggtcgtca ccgatttctc
    10081 gtcggacaag caggcattcc acgtcccctg gggcaaggcg atgatgtgga tcttcctgct
    10141 gagcgacacg ttcatcttca gctgtttcct ggtgtcgtac atgaccgtcc gcatgtcggc
    10201 ggcggagccg tggccctatc ccagtgaggt gttcgcgctg agcttcggcg gtgatccgat
    10261 cccgctcgtg ctgatcgcca tcatgacttt cgtgctgatc acctccagcg gcacgatggc
    10321 aatggctgtc aacatggggt atcgcaagga ccgcaccaag accttctggc tcatggtggc
    10381 aacggctgta cttggcgcca ccttcgtcag catgcaggcg ttcgagtgga ccaagctgat
    10441 cgtggacgag ggcgtgcgcc cctggggcaa cccgatgggc gctgcgcagt tcggttccag
    10501 cttcttcatg attaccggct tccacggcat gcacgtgtcg gccggcgtca tctacctgct
    10561 ggtggtcgcg ttcc
//
LOCUS       DASUOM010000053         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_353278,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000053 DASUOM010000000
VERSION     DASUOM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353278"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFY27_01165"
     CDS             complement(<1..1136)
                     /locus_tag="VFY27_01165"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HEX5764147.1"
                     /translation="MQVDRDAAPSVSYLSMIENGKRVPSERLLQVVAGIFQKEVSWFF
                     DETLQAETVETTPRTGGIEGIPLEPGFLFSNDLLQTAIPELLSQTGTTGRQFAHLLIR
                     AHQESHQNRFPDLERAAEAIGKKRFPLTVEDLFGMARRLGLRIRWFDHPPFRDRSGGQ
                     RPLDTLVRSFYEAPNVIYLNRQLETLPGRLKYDIGNHIAHRILHDGDGARTPQVAGGD
                     ISPRRQDVASPTMEAKDILYAWRDFECSYFASALLGPKTPFRQFLTRYAYSIDVGKKI
                     DLSTTLIMRRMSSVSPYPHWHYFDAYPPGNLRAVYRGNGIPLPWGNMRMVLDPCQHWA
                     VFKMLNTRSTKPSAQISVLRSGSDARLYCCQSIRSKDPAGNAHVL"
     gene            1384..2664
                     /gene="aceA"
                     /locus_tag="VFY27_01170"
     CDS             1384..2664
                     /gene="aceA"
                     /locus_tag="VFY27_01170"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX5764148.1"
                     /translation="MSRDTQIAELEKEWAESPRWKGVKRDYRAADVVKLRGSVLVEHT
                     LARLGAEKLWRLVHQEEPLCALGAITGNQAIQEVQAGLKAIYCSGWQVAGDGNSSGQM
                     YPDQSLYPVDSVPKMVERINNAFTRTDEIYSLNGDDSIDWFAPIVADAEAGFGGNLNA
                     FELMKHMIRSGAAGVHFEDQLSSAKKCGHMGGKVLVPTHEAVAKLSAARLAADVLGVP
                     TVLIARTDADAANLITSDADERDRDFINGERTAEGFFRTRAGLDQAIARGLAYAPYAD
                     MIWCETSKPDLEQARKFADAIHAQFPGQLLAYNCSPSFNWKANLDDRTMACFREELGA
                     MGYKFQFITLAGWHALNSSMFKLSRAYKADGMFAYSRLQQEEFADEEHGFRATKHQSF
                     VGAGYFDAVQNIIMSGQSSTTAMDGSTEEEQFAA"
     gene            2790..3770
                     /locus_tag="VFY27_01175"
     CDS             2790..3770
                     /locus_tag="VFY27_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019674783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5764149.1"
                     /translation="MKLASLKTGGRDGTLVVVSRDLTRAVAVRDIAPTLQSVLDDWDQ
                     TAPLLQEVAEHLEQGAIAREFTFDPASVAAPLPRAYHWVDGSAYVNHVELVRKARGAG
                     MPDSFWHDPLVYQGGSDDLLGPCDDIQVASEDFGIDMEAETAVITDDVPMGTRAEDAG
                     EHIKLLMLVNDVSLRNLIPAELAKGFGFYQSKPSTAFSPVAVTPDELGEAFAEGKFHL
                     PLRTSLNGQLFGEPDCGVDMTFDYHQLIEHVTRTRRLGAGAIIGSGTISNYDRSKGSS
                     CIAEKRMLETIEDGKPATPFLKFGDVVRIEMLDGEGRSIFGAIEQKVVRA"
     gene            3803..5131
                     /gene="hmgA"
                     /locus_tag="VFY27_01180"
     CDS             3803..5131
                     /gene="hmgA"
                     /locus_tag="VFY27_01180"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5764150.1"
                     /translation="MMPSDPLSNRRSLEYMSGFSNEFATEALAGTLPVGRNSPQRVAH
                     GLYAEKLSGTAFTAPAKDNYRSWLYRIRPSVVHGDFTRIDGGLIRTGPDGEGTMTPDQ
                     LRWDPLVIPENATDFIDGMVTMATNGDVRLQAGIGIHVYRATSSMQDRFFYNSDGEML
                     LVPEQGDIAIHTECGVLAVGPGEIAIIPRGMKFRVALLHDVARGYVCENYGRKFELPE
                     RGPIGGDGLANRRDFETPVAAYEDREGDFELVTKLGGALFHCRIGHSPLDVVAWHGNL
                     VPCKYDLRRFNVIGSISHDHPDPSIFTVLTSQSDTRGTANADFVIFPPRWLVMEDTFR
                     PPWYHRNVMSEYMGLIYGEYDAKSGGGFVPGGASLHNCMSAHGPDRTTFDQASAAKLE
                     PLKLERTMAFMFESRYLLQPTRFALACPELQKDYRDCWQNLERRFPAQSP"
BASE COUNT         1135 a         1644 c         1773 g         1090 t
ORIGIN      
        1 aggacgtggg cgttaccggc cgggtccttg ctgcggatgg actggcagca gtagaggcgg
       61 gcgtcgctgc cgcttcgcag gacggagatc tgggcggagg gtttggtgga gcgggtgttg
      121 agcattttga agaccgccca gtgctggcag ggatcgagga ccatgcgcat gttgccccag
      181 ggaagcggaa tgccgttgcc gcgatacacg gcgcggagat tgcctggcgg gtaagcgtcg
      241 aagtaatgcc agtgcgggta cggagagacg ctggacatgc gtcgcatgat caaagtcgta
      301 gagaggtcga tctttttgcc gacgtctatc gaataggcgt agcgggtcag gaactggcga
      361 aagggcgtct tgggcccgag tagcgctgag gcaaaatagc tgcattcgaa gtcgcgccag
      421 gcatacagga tgtccttagc ctccatcgtg ggcgacgcga catcctgacg tctgggtgag
      481 atgtcgcctc ccgcaacctg cggcgtgcgt gcgccgtcgc cgtcgtgcag gatgcggtga
      541 gctatgtggt tgccgatgtc gtacttcagg cggcctggaa gggtttcgag ttgacgattg
      601 aggtatatga cgttcggcgc ttcgtagaac gagcgtacga gcgtgtcgag tggccgttgc
      661 cccccgctcc tgtcgcggaa cggcgggtga tcgaaccagc ggatgcgcag gcccaggcgg
      721 cgtgccatgc cgaagagatc ctcgaccgtc aggggaaagc gctttttacc gatcgcttcc
      781 gctgctcgtt cgagatccgg aaaccggttc tggtgtgact cctggtgagc acgaatcaac
      841 aggtgtgcaa actgccgacc ggtcgtgccg gtctgcgaca gcagctcggg tatcgcggtc
      901 tgcagaaggt cgttcgaaaa caggaaacct ggttcgagag gaatgccctc gatcccgcct
      961 gtcctgggag tcgtctcgac cgtttctgcc tgtagcgttt cgtcgaaaaa ccaggagacc
     1021 tctttctgga aaattccggc taccacctgc agcaagcgct ccgacggcac gcgtttgccg
     1081 ttttcgatca tcgagaggta ggaaacggag ggcgccgcat cgcgatcgac ctgcatacat
     1141 cgcacggcca gatcttcaag tgtcaggtga tttcttttcc gcaagctttt gattttgctg
     1201 ccaaggaagt gtgcttttcg tagaaacccg tgcccgcctg ccattatgtc accctgtgaa
     1261 attaacactg tgaaattttt attgtgaaat tatatgctgc agctggctaa gatgctcgtg
     1321 atgcagtgca caggagtgca cgggttgctt tgggcttttc cgcattgacg caggagaccg
     1381 accatgtcac gtgacacgca aatcgccgaa ctggaaaaag aatgggccga gagcccgcga
     1441 tggaagggag taaagaggga ctatcgcgcc gcggacgtgg tgaagctgcg gggctccgtg
     1501 ctcgtcgaac atacgctcgc ccgcctcggc gcggaaaaac tctggcgcct cgtgcaccag
     1561 gaagagccgc tgtgcgcgct cggcgctatc acgggcaacc aggctatcca ggaagtgcag
     1621 gccggcctca aggcgatcta ctgttccggc tggcaggtcg ccggtgacgg caacagctcc
     1681 ggccaaatgt atcccgacca aagtctctat cccgtcgact cggtgccgaa gatggtggag
     1741 cgcatcaaca acgcgtttac ccgcaccgac gagatctact cgctcaatgg cgatgacagc
     1801 atcgactggt ttgcgccgat cgttgcggat gcggaagccg gcttcggcgg aaacctgaac
     1861 gcatttgaat tgatgaagca catgatccgc tcgggtgccg ccggcgtgca tttcgaagac
     1921 cagctgtcat ccgcgaaaaa gtgcgggcac atgggcggca aagtgctggt tcccacgcac
     1981 gaggcagtag caaagctttc ggcggcacgc ctcgctgccg acgtcctggg ggtgccgacg
     2041 gttctcatcg cccgaaccga cgccgatgca gcgaacctga tcacgtcgga tgcagacgag
     2101 cgggatcgcg atttcatcaa cggcgagcgc acggcggaag gttttttccg cacccgcgct
     2161 gggctcgacc aggccatcgc caggggcctt gcttacgctc cctatgcgga catgatctgg
     2221 tgtgaaacgt cgaagccgga cctcgagcag gcgcggaaat ttgcggatgc catccatgca
     2281 cagttccctg gccagctgct ggcgtacaac tgctcgccct ctttcaactg gaaagcgaac
     2341 cttgacgacc gcacgatggc gtgcttccgc gaggaattgg gcgcgatggg ctacaagttc
     2401 cagttcatca cgctggcagg ctggcacgcg ctgaactcga gcatgttcaa gttgagccgg
     2461 gcctacaagg ccgacggcat gttcgcgtac tcgaggctgc agcaggaaga atttgcggac
     2521 gaggagcatg gcttccgtgc cacgaagcac cagtcgttcg tcggcgcggg ttacttcgat
     2581 gccgtacaga acatcatcat gtcaggccaa tcctccacca ccgccatgga tggcagcacc
     2641 gaagaggaac agttcgccgc ctgatccctc gccggccagg cggcatgcgc acgaggcgtc
     2701 accgggagca ggagggttgg agtcccggcg aatgaacgtc taatatcccc ttatagataa
     2761 taagaagaac gtgcataagg gataggtatt tgaagctggc aagcctgaag accggcggcc
     2821 gggacggcac ccttgtcgtc gtcagccgcg acctgactcg tgcagtcgcc gtccgcgaca
     2881 tcgcgccgac gctgcaatcg gtgctggacg actgggacca gaccgcgccg ctccttcagg
     2941 aggtcgccga gcacctggag cagggggcca tagcccggga attcacgttc gaccccgcgt
     3001 ccgtggcggc gccgctgccc cgggcgtatc actgggtcga cgggagtgct tatgtaaacc
     3061 acgtcgaact cgtgcgcaaa gcaaggggag ccgggatgcc ggacagtttc tggcacgacc
     3121 ctctcgtgta ccagggcggc tccgatgacc ttctcgggcc ttgcgacgac atccaagtcg
     3181 ccagcgagga cttcggcatc gacatggaag cggaaaccgc cgtcataacg gatgacgtgc
     3241 caatggggac ccgggcggag gatgccgggg agcacataaa gctgctgatg ctcgtcaacg
     3301 acgtctcgct gcgaaacctg atacccgccg aactggcgaa aggcttcggc ttttaccagt
     3361 cgaaaccctc gaccgccttc tccccggtcg cggttacccc tgacgaactc ggcgaagcct
     3421 ttgccgaagg caagtttcat ctccccttga ggaccagctt gaacggacag ctgtttggcg
     3481 aacccgactg cggcgtcgat atgacgttcg actaccacca gctgatcgaa catgtgacga
     3541 ggacgcggcg gctcggcgcc ggagcgatta tcggctccgg aaccatatcc aactacgacc
     3601 gctcaaaagg ttccagctgt atcgccgaga agcgtatgct ggaaacgatc gaagacggca
     3661 agcccgcgac gccattcctg aagttcgggg atgtggtgcg catcgagatg ctggacgggg
     3721 aagggcgtag tatcttcgga gccatcgagc agaaagttgt ccgggcctga gccaggacac
     3781 cggcattccc atcgagaaac gcatgatgcc aagtgatccg ctgtcgaacc gtcggtcact
     3841 cgaatatatg agtggcttct ccaacgaatt cgcgacggag gcgctcgccg gcacgctgcc
     3901 tgtcggcagg aactcgccac aaagggtagc gcacggcctt tatgccgaaa agctgagcgg
     3961 taccgcgttc acggcgcccg caaaagacaa ttaccggagc tggctttacc gcattcgccc
     4021 gtcggtggtg catggcgatt tcacacggat cgacggcgga ctgatccgca ccgggcccga
     4081 cggcgaaggg acaatgacgc cggaccagct gcgctgggat ccgctcgtca tccccgaaaa
     4141 cgcaaccgat tttatcgacg gcatggtcac gatggcgacc aacggtgatg tgcgccttca
     4201 ggccggcatc ggcatccatg tgtatcgcgc caccagctcg atgcaggacc gctttttcta
     4261 caactccgac ggtgaaatgc tgctggtacc ggagcagggt gatatcgcaa ttcacaccga
     4321 gtgtggggtg cttgccgtcg ggccgggtga gattgcaatc atcccgcgcg gaatgaagtt
     4381 ccgcgtcgcc ttgttgcatg acgttgcccg cggttacgtg tgcgagaact acggacgcaa
     4441 attcgaactg ccggaacgcg gcccgatcgg cggcgacggg cttgcaaacc gccgcgactt
     4501 cgagacgccg gtagccgcct acgaagacag ggagggggat ttcgagctgg taacgaagct
     4561 cggcggcgcg ttgtttcatt gccgcattgg ccactcgccg ctggacgtcg ttgcctggca
     4621 tggcaacctg gttccctgca aatacgacct ccggcgattc aacgtcattg gctcgatcag
     4681 tcacgatcat ccggacccgt cgatttttac cgtactgact tcgcagtcgg acacgcgcgg
     4741 aacggcgaac gccgacttcg tcattttccc cccgcgctgg ctggtcatgg aggacacgtt
     4801 tcggccaccc tggtatcacc ggaacgtgat gtcggaatac atggggctga tatacggcga
     4861 atatgacgcg aagagcggcg gcggatttgt ccccggcgga gccagtctgc acaactgcat
     4921 gtcggcgcac gggccggatc gcaccacctt cgaccaggcc agcgccgcga aactcgaacc
     4981 cctcaaactg gagcggacca tggccttcat gttcgagtcg cgatacctgc tgcagccgac
     5041 acgcttcgca ttggcgtgtc ccgagctgca aaaagactac cgtgactgct ggcaaaatct
     5101 cgaacggcga tttcccgcac agtcgcccta gcggcagtac tttgagcgca gcttgcgcag
     5161 ctcgcgcagt cttgcttcca tcctctctcc ctcggacgcg ccgtatccct gcctcatttt
     5221 tgcctggatc ctgagaatct cgcgcctggt cttttcgcag tctgccttgc aggctgattt
     5281 cggatagcag ctaccggaag ccaaacctgc gttggaaggc aaggttgcca gcagcaaaat
     5341 aaatcccgcc acgataatct ttcgcatacg tcggccggtg gtctgtcaga cttcgaataa
     5401 gtgtcgcgtg gtgcggaggc gcaggcgatc ggcgaaaggc ggcgaatgac gcggttttgt
     5461 gcgattgctt cctggtggca gaatgcagaa ggaggcgaaa caggatgatt ctcgtttcaa
     5521 caacgttgtg ccgggcgcgg gtgtaatacg tgccgagact cgtgccgtcg tggctgttac
     5581 tgtcggtcgc cgtggccgtc gtgctgtggt ctctcgcgac cttgctcgag aaccgcctgc
     5641 ag
//
LOCUS       DASUOM010000054         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_582869,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000054 DASUOM010000000
VERSION     DASUOM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582869"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFY27_01185"
     CDS             <1..382
                     /locus_tag="VFY27_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5764151.1"
                     /translation="VADLEAALADAATEDRPAMLDFYADWCVSCIEMEHYTFTDPNVQ
                     EALADTVLLQADVTANDQQDQALLKRFGVFGPPTIIFFGSDGTERDGFEVVGFMDAQE
                     FAAHVQRATGAGATATIAAQQPGT"
     gene            476..1048
                     /locus_tag="VFY27_01190"
     CDS             476..1048
                     /locus_tag="VFY27_01190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5764152.1"
                     /translation="MTKKILVVLVLIAAAGAGYWLAQRTSDQAVASYQDRQISFALPD
                     LGGNMRDLAEWEGKTRLVNFWATWCAPCRREIPLLKEMQAKHGADGLQVIGVAVDYQE
                     DVVAYAETADFNYPILIGQEDAMAAAETSGMDFVGLPVTMLVSPEGDLIKMHMGEIME
                     NHIEQIVDVVARMQSGELDLNGAREALGRF"
     gene            1183..1629
                     /gene="aroQ"
                     /locus_tag="VFY27_01195"
     CDS             1183..1629
                     /gene="aroQ"
                     /locus_tag="VFY27_01195"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5764153.1"
                     /translation="MASLLLINGPNLNLLGSREPQVYGRTSLSEIERELCAKAESLGH
                     TLACFQSNGEEKIIDRIQKASADSVDFILLNPGALTHTSIAIRDALLGVGIPFIEIHL
                     SNVFAREEFRRHSYLSDIAAGCLFGLGASGYELALLAAHSKLANRG"
     gene            1669..2154
                     /gene="accB"
                     /locus_tag="VFY27_01200"
     CDS             1669..2154
                     /gene="accB"
                     /locus_tag="VFY27_01200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5764154.1"
                     /translation="MDIRKVKKLIELLDESGIAEIEITEGEESVRISRYSPGYAPQMP
                     PLQYAQPQTSAAAAPVSDRQASAGSPSTVEAEPEEDGFAVVAPMVGTFYSAASPGSAP
                     FVQVGDRVNQGDTLCIVEAMKMMNQIEAEVSGMIKSIRVQNGDPVEYGQVLFIIDQRG
                     T"
     gene            2161..>3007
                     /locus_tag="VFY27_01205"
     CDS             2161..>3007
                     /locus_tag="VFY27_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5764155.1"
                     /translation="MLEKVVIANRGEIALRILRACREMGIKTVAVHSTADNNLKHVLL
                     ADETVCIGPPSSTESYLNMPAIISAAEVTDSVAVHPGYGFLAENADFAERVESSGFIF
                     IGPPASAIRLMGDKVSAIRAMKAAGVPCVPGSDGPVSENPDENLRLARDIGYPIIIKA
                     SGGGGGRGMRVVHSEASLIGAITVTQAEARSAFGNETVYMEKFLEKPRHIEFQVLADG
                     RGNAVHLAERDCSMQRRHQKVIEEAPAPGITEEQRQKIGQRCVEACLEMGYRSAGTFE
                     FLYQDE"
BASE COUNT          627 a          906 c          928 g          546 t
ORIGIN      
        1 ggttgcggat ctcgaagcgg cgctcgcgga cgcggccacg gaggacaggc cggcgatgct
       61 cgatttctac gcggactggt gcgtatcctg catcgaaatg gagcactaca cttttaccga
      121 tcccaatgtc caggaagctc tggcggacac agtattgctc caggccgacg taacggccaa
      181 cgaccagcag gaccaggctt tgctgaagcg tttcggcgtc ttcggcccgc cgaccatcat
      241 attcttcggc tctgacggta cagaacgaga cggcttcgag gtggtcggat tcatggatgc
      301 gcaggaattc gcggctcacg tgcaacgcgc caccggcgcc ggagcaaccg ccacgatcgc
      361 tgcgcagcag ccgggcacct gacccggtca tacatttcac ccggggccgc ttttgcttga
      421 tcctgcgagg tcgagcagat ccatcatcgt ccctggtcaa acaacaaggc tcgcaatgac
      481 gaagaagata ctcgtggtcc tggtgctcat cgccgcggcg ggcgcaggtt actggttagc
      541 gcagcggacg tccgaccaag cggttgcttc ctaccaggac cggcagattt ccttcgcgct
      601 acccgacctt ggcggcaaca tgcgcgacct cgccgaatgg gaggggaaga cccgcctcgt
      661 gaacttctgg gcgacctggt gcgcgccgtg ccggcgtgag attccgctgc tcaaggaaat
      721 gcaggcaaag cacggtgcag acggactgca ggtgatcggc gtcgctgtcg attaccagga
      781 agacgtcgtc gcctatgccg aaaccgcgga cttcaattac ccgattctca tcggccagga
      841 ggacgcaatg gcggcggcgg aaacctccgg catggatttc gtcggcctgc cggtgacaat
      901 gctcgtgtct ccagaggggg acctgatcaa gatgcacatg ggcgaaataa tggaaaatca
      961 catcgagcag atcgttgacg tggtggcccg gatgcagagc ggcgaactgg acctcaacgg
     1021 tgcccgcgag gcactcggca ggttttagga caagtccggc cgggccatat cggccacggt
     1081 agcgcggtca gggtcccatt cctgcgtgcc ggagttgcat gagctgccgg gaaaagggtt
     1141 agaattgccg ccatcttcgt caaactggct gcgaatcctg acatggcgag cctcttgctc
     1201 atcaacgggc ccaacctcaa cctgctcggc agccgggagc cgcaggtcta tggccgcacc
     1261 agcctgtccg agatcgaacg ggaactgtgc gcaaaggccg agtccctggg ccatacgctc
     1321 gcgtgctttc aaagcaacgg cgaagaaaaa atcatcgacc gcatccagaa ggcgtcggct
     1381 gacagcgtgg acttcatcct gctcaatccg ggcgccttga cccataccag catcgcgatc
     1441 cgtgacgccc tgttgggcgt cggcattccc tttatcgaaa ttcacctgag caacgtcttc
     1501 gcccgggagg agttccgccg ccacagttac ctgagcgata tcgcggcggg ttgccttttt
     1561 ggcctcggcg cctccggtta cgaactggca ttgcttgcag cacattcgaa actcgcgaac
     1621 cgcgggtagt gcgatcacga atacattcgg cagttccggg atcaggaaat ggacatacgt
     1681 aaagtaaaaa aactaatcga gctgctggat gagtccggta tcgcggagat tgaaatcacc
     1741 gaaggggaag agtccgtcag gatcagccga tacagccccg gctatgcacc gcaaatgccg
     1801 ccgcttcagt atgcgcaacc gcagacgtca gcggccgcgg cgcccgtctc cgaccggcag
     1861 gcatctgccg gttcgccgtc gacggtagag gcagaacccg aagaagatgg tttcgccgtc
     1921 gtcgcaccga tggtcggcac cttctacagc gccgcctctc ccggttccgc tccgttcgtc
     1981 caggtaggag accgagtcaa ccagggagac acgctttgca tcgtcgaagc gatgaagatg
     2041 atgaaccaga ttgaagccga agtttcgggc atgatcaaat cgatacgcgt acaaaacggg
     2101 gacccggtcg agtacgggca ggttctgttc atcatcgacc agcgcggcac ttaagccttc
     2161 atgctcgaaa aggtcgttat tgcgaatcgc ggcgagattg cgctgcgcat acttcgtgcc
     2221 tgccgcgaga tgggcatcaa gacggtggcc gtccattcca cggccgacaa taacctgaag
     2281 cacgttctcc tcgccgacga aactgtctgc ataggccccc cgtcatcgac ggaaagctac
     2341 ctgaacatgc ccgcgatcat cagtgccgcg gaagtgacgg actcggtcgc tgtgcatccc
     2401 ggttacggct tcctggccga gaacgcggat tttgcggagc gagtggagtc gagcggcttc
     2461 attttcattg gcccgccggc atccgcgatc cggctgatgg gcgacaaggt gtccgccatc
     2521 cgcgcgatga aagcggcggg agtaccttgt gtgccgggct ccgacggccc ggtatcggaa
     2581 aacccggacg agaacctgcg cctggcgcgc gatatcggct atcccatcat catcaaggcg
     2641 tccggaggcg gtggcgggcg cgggatgcgc gtcgtgcatt cggaggcttc cctgatcgga
     2701 gccatcacgg ttacccaggc ggaagctcgt tcagccttcg gcaatgaaac cgtctacatg
     2761 gaaaagttcc tcgagaaacc ccgacacatc gagttccagg tcctcgcgga cgggcgcggg
     2821 aatgccgttc acctggccga gcgcgattgc tcgatgcagc gccggcacca aaaggtaatt
     2881 gaagaagcac cggcgccggg gatcacggag gaacagcggc aaaagatcgg ccagcgctgc
     2941 gtggaggcct gtctcgagat gggctaccgc agcgccggca ccttcgagtt cctttaccag
     3001 gatgagg
//
LOCUS       DASUOM010000055         5905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1670583,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000055 DASUOM010000000
VERSION     DASUOM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670583"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFY27_01210"
     CDS             <1..724
                     /locus_tag="VFY27_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5764156.1"
                     /translation="GSGSDAITLSSQKILAKRWMRLNGIPTPEWHSAAEFADGREDEN
                     RSWIVKSLWEHASFGIDDASVVRGRGAAEQRIRQCIDTYGGRWFAEEYVDGREFNISI
                     VEQKGQPLVLPIAEMTFVGFPPDKPKIVGYQAKWNEGAPEYAATQRRFEVSGADRDVL
                     TAVAKKCWMLFGLRGYARVDVRMDQQGTPFVLEVNANPCLAKDAGFIAAATEAGFRYR
                     QVIEMVLHAAIRPVLPVLRRAG"
     gene            751..2529
                     /locus_tag="VFY27_01215"
     CDS             751..2529
                     /locus_tag="VFY27_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5764157.1"
                     /translation="MFRIHKIFDVTTPANQRLLSQVQAMLRVQFEALSEQEIETLPAR
                     LQHRHRYRFRPILLVAEKGPSVRGFALLLHDPGLGFCLLDYVAAARGKTGGGIGGALY
                     ERAREEAYQLGVVGIFLEALPDDPALSPDPAVRKQNAARLKFYESFGARPIVGTAYET
                     PFRETDTDPPYLVFDNLGRDVQLRSAQARKIVRTILERKYGPVAPPGYIDKVVESFRD
                     DPVRLRQPRYVREPAGAQDRRPKPRKIALVVNDRHDIHHVRDRGYVEAPVRIASILKE
                     LDRTLIFERMAPRHFGVKHIEQVHDPEFVRYLQKACANVPESNSVYPYVFPVRNRARP
                     PRHLPLRAGYYCIDTFTPLNANAYKAALGAVDCALTGADCLLDGGRAAYALVRPPGHH
                     AERRSFGGFCYFNSAAVAANYLSEHGRVALLDIDYHHGNGSQDIFWRRRDVLTVSIHG
                     NPRFTYPYFSGFEEEKGESDGAGCNVNLPLPENIDGERYRETLAAALRIVRKFEPQFL
                     VLALGLDTAKGDPTGSFRLTARDFSENGRLIGALRLPMLVVQEGGYKTQTLGVNARHF
                     FTGLWENLSPPDAEQTAGQNRRGSKR"
     gene            2529..3014
                     /locus_tag="VFY27_01220"
     CDS             2529..3014
                     /locus_tag="VFY27_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5764158.1"
                     /translation="MQWRDQVCDNDRLAVRQLVTATGFFSPAEQDIAVELVDDTLTQG
                     EAAGYEFLFADNEERPGMLRGYACFGPIPSRSGDYDLYWIAVAPFGQRHGLGRQLVAE
                     AERRAVSRGAVSMFIDTSGREQYLPTRAFYERMGYRIHEVVRDFYSPGDDKVVYRKQL
                     C"
     gene            3097..4074
                     /locus_tag="VFY27_01225"
     CDS             3097..4074
                     /locus_tag="VFY27_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5764159.1"
                     /translation="MKFRSILCAVALSLTGPYAVAQDQAPAEEETSRDDQAVVESTPP
                     QSDAADPLKIFISADMEGVVGVVTDEQIGPGGFEYERFRGFMTAEVNACIEAAREAGA
                     TEILIADSHGNGQNLLIERLPDDVMLVRSWPRPLGMMEGIDESFDGVIFLGYHASTGN
                     ERGVRAHTLSSANVTALRLNGMSMSEASINAAIAGHFGVPVIMVSGDNVAVAETQVII
                     GDVEGAVVKWAKGFHSAQTLMPAAAYDVIRERTREAMRRIEQFEPYVLETPVRLELSL
                     KHYRPVELLSYLPGVERINSHTIGFVGENMLEVSRFLRVALGYSVDLQP"
     gene            complement(4087..>5905)
                     /locus_tag="VFY27_01230"
     CDS             complement(4087..>5905)
                     /locus_tag="VFY27_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764160.1"
                     /translation="QLMADVVRTSEEFLDSEGRLYFHGESFDNYYIGKGSTFPFVNGG
                     VGILYEAAAALGVEIDTANGLRTYRENIRKHFRTSLASIESAASLRAELLQYQKEFYD
                     SAIAEADDAAVQAYVFAAPGDPARMFHFLDMLQSHRIRAWRLGREVTEGGVTFSPGEA
                     VVVPVSQPQFRLIRGMFETVSEFEDTTFYDVSTWNIPLAFDLDFAPLSGRRFNENLLG
                     ETVAAAMPVADTPDTSGYAYAFEWSGYYAPRALNRLLEEEILARVATKPFTGRTSRGP
                     VSLARGTILVPYDRQEKSRAEIREVMEAIAREDGIPVHALESGASAAGGESIDAGAPS
                     FKPLKQPEVLLVVGRDMDLYDAGEIWHLLDHRMRMRVTLRDRDQLDGINWSGYTHIVF
                     AGGVYDEYLPDFLPRLRQWVSEGGTLVALREAALWARTNVLDFIDPEEGVLGPEPGAP
                     EAAPETPSGHDPEETDEILEPYRFPYSEKETRDALELIGGAIFAGDLDNTHPIGFGYR
                     DRNIALHKNLEDIMERPENPFATVIAYDTPPVLSGYTSEANRIMLEGTAALIAERRQQ
                     GSIILFADNPNFRGYWYGTNKLFLNALFFSKAFDPLPEE"
BASE COUNT         1130 a         1798 c         1887 g         1090 t
ORIGIN      
        1 cgggtccggc agcgacgcga taaccttgag ctcgcagaag atccttgcca agcgctggat
       61 gcggctcaac ggaattccta cgccggagtg gcattctgcg gcggagttcg cagacggccg
      121 cgaagacgaa aaccgttcgt ggatcgtgaa atccctttgg gagcacgctt cgttcgggat
      181 cgacgacgcc tccgtggtcc gtggacgggg agcggccgag cagcgcatcc ggcagtgcat
      241 cgacacttac ggcgggcgat ggtttgccga ggaatacgtc gacggccgtg agttcaacat
      301 ttcgatcgtt gaacagaaag ggcagcccct cgtgctgccg atcgcggaga tgaccttcgt
      361 cggtttcccg ccggacaagc cgaagatcgt gggctaccag gcaaaatgga acgaaggcgc
      421 accggagtat gcagcgacgc aacgccggtt cgaggtttcc ggtgcggatc gggatgtcct
      481 taccgcggtc gccaagaagt gctggatgct gttcggactg cgcggctatg cgcgggtcga
      541 tgtccggatg gatcagcagg gcacgccctt cgtactggaa gtgaacgcca atccctgcct
      601 ggcgaaggat gcaggcttca tcgccgcagc gaccgaagca ggctttcgct accggcaggt
      661 gattgaaatg gtactgcacg cggcgattcg tcccgtgctg cccgtattgc gccgtgccgg
      721 ctgaagtctc cggcaggtga tcacgctctc gtgttccgca tccacaagat tttcgatgta
      781 accacgcctg caaaccagcg cctgttaagc caggtccagg cgatgctccg cgtccagttc
      841 gaagccttga gcgaacagga gatagagacc ctgccggcac gcctccagca ccggcaccgg
      901 taccgcttcc gtcccatcct gctcgttgcc gagaagggcc cttccgtgcg gggtttcgcc
      961 ctgctgctgc acgaccctgg gctcgggttc tgcctgctgg actatgtggc cgcggctcgg
     1021 ggaaagaccg gcggcggcat cggcggtgcg ctgtacgaac gtgcccgcga agaagcgtac
     1081 cagcttggcg tagtcggtat tttcctcgaa gcgttgccgg atgacccggc gctgtctccc
     1141 gatccggcgg tgcggaagca gaatgccgcg cgcctgaaat tctacgagag cttcggcgca
     1201 cgtccgatcg tcggaaccgc ctacgagacg cccttcaggg agacggatac cgatccgccc
     1261 tacctggtgt tcgacaacct cggccgcgat gtgcagctaa ggagcgcgca ggcgcggaag
     1321 atcgtacgca ctatcctgga aaggaagtac ggcccggttg ctccccccgg ttacatcgac
     1381 aaagtggtcg aatccttccg ggacgacccg gtgcgcctgc gacagccacg gtacgtcagg
     1441 gagccggcag gtgcccagga ccggaggccg aagccgagaa agattgcgct ggtggtgaac
     1501 gaccggcacg acatccacca tgtccgcgac cgcggctacg tagaagcgcc tgtccggatt
     1561 gcgtcgatcc tgaaggagct ggaccgaacc ctgattttcg agaggatggc gccgcgccat
     1621 ttcggcgtga agcacatcga gcaggtgcac gatccggaat tcgtacgcta cctgcagaag
     1681 gcatgcgcga acgtaccgga atcgaactcc gtctatccct acgtcttccc cgtccgtaac
     1741 cgggcgcgac cgccgaggca cctgccactg cgggcgggtt actactgcat cgataccttc
     1801 acgccgctga acgccaatgc gtacaaggcg gcgctcggcg ccgtcgactg cgccctgacc
     1861 ggcgcggatt gcctgctcga tggaggacgc gccgcctacg cgctcgttcg tccgcccggc
     1921 catcacgctg aacgccgctc cttcggcggc ttttgctatt tcaattctgc ggcagtggcg
     1981 gcgaactacc tgagcgagca cggccgcgtt gcgctgctcg acatcgacta ccaccacggg
     2041 aacggctcgc aggacatctt ctggcgccgc cgtgacgtgc tcaccgtgtc catacacggc
     2101 aatccgcgtt ttacctaccc gtatttctcg ggcttcgagg aggaaaaggg cgagagtgac
     2161 ggcgcgggtt gcaacgtgaa cctgccgttg ccggaaaaca ttgacggcga gcgctaccgc
     2221 gagacgctgg cggccgcact gcgcatcgtg cgaaagttcg agccccaatt cctcgtgctg
     2281 gccctgggac tcgatacggc gaaaggcgac ccgacaggtt cgttccggct gaccgcgaga
     2341 gacttcagcg agaacggcag gctcatcggg gccttaaggc tgccgatgct ggtggtgcag
     2401 gagggtggat acaagaccca gacgctcggc gtcaacgcga ggcacttctt cacggggctg
     2461 tgggaaaacc tgtcgcctcc ggatgcggag caaaccgcgg gtcagaaccg ccgcggcagc
     2521 aagcgttgat gcagtggcga gaccaggttt gcgacaacga tcggctcgcc gtgcggcaac
     2581 tcgtgacggc aaccggcttc ttctctcccg cagagcagga cattgccgtg gagctggtcg
     2641 acgacacgct gacccagggc gaagcggcag gctacgagtt cctgtttgcc gataacgaag
     2701 aacggcccgg gatgctgcgc ggttacgcct gcttcgggcc gatcccttca cgttccggcg
     2761 actacgacct ctactggatt gccgtggcac cattcggcca gcgtcacggc ctcgggcggc
     2821 agctggttgc agaggccgaa cgccgcgcag tttcaagagg cgccgtcagc atgttcatcg
     2881 atacctcggg gcgtgagcag tacctgccga cccgtgcatt ttatgaacgc atgggatacc
     2941 gaattcacga ggtagtgcgc gatttctatt ctccgggcga cgacaaggtc gtgtaccgca
     3001 agcagctttg ctaaagcttc agcttttgac cctccaggga cgcaggcata tcctccgtga
     3061 gtgacaccga ctgagctctc gataaggagt agcggaatga agttcagaag tatcctctgc
     3121 gcagtcgctc tttcgctgac gggaccctat gccgtggcac aggaccaggc accggccgag
     3181 gaggaaacat cgcgcgatga tcaggcggtg gtcgaatcga cgccgccgca gtcggatgct
     3241 gcggacccgc tcaagatatt catatccgcg gacatggaag gcgtggtcgg tgttgtcacg
     3301 gacgagcaaa ttggcccggg tggcttcgag tacgagcgct tccgcggttt catgaccgcg
     3361 gaggtcaacg catgcatcga agcggcgcgc gaagctggtg caaccgagat actcatcgcc
     3421 gactcgcacg gcaacgggca gaacctgctg atcgaacgcc tgcccgatga cgtgatgctg
     3481 gttcgttcat ggccccggcc gctcggcatg atggaaggca tagacgagtc gttcgatggc
     3541 gttatctttc tcggctatca cgccagcacc gggaacgagc gcggcgtgcg ggcccacaca
     3601 ctttcgagcg ccaacgttac cgcgctgcgc ttgaacggca tgtcgatgtc cgaggccagc
     3661 atcaacgcgg cgatcgccgg tcacttcggc gtgcccgtga tcatggtctc gggcgacaac
     3721 gtggcggtcg ccgaaaccca ggtgatcatc ggggatgtcg aaggcgcggt cgtgaagtgg
     3781 gcaaaaggct tccactccgc gcagacgctg atgcccgcag ccgcatacga cgttatccgc
     3841 gagagaacgc gggaagcgat gcggcggatc gagcagttcg aaccctacgt actggaaaca
     3901 cccgtgcgtc tcgagctaag cctcaagcac taccggccgg tggagctgct ttcgtacctg
     3961 ccgggagtag aacgcatcaa ttcacacacc atcgggtttg tcggcgaaaa tatgctggag
     4021 gtgagcaggt tcctgcgggt tgccctgggc tactccgtgg acctgcaacc ctgattcaac
     4081 tgcgcgtcac tcctccggca acggatcgaa ggccttcgaa aaaaacagcg cgttgagaaa
     4141 cagcttgttc gtcccgtacc agtagccgcg aaagttcggg ttgtcggcga acaggatgat
     4201 gctgccctgc tgcctgcgct ctgcgatcag cgcggcggtg ccttcgagca tgatgcgatt
     4261 ggcctcggac gtatatccgg agagcacggg gggcgtgtca taggcaatga cggtggcaaa
     4321 cgggttctcc ggacgctcca ttatgtcctc caggttcttg tgcagggcga tattcctgtc
     4381 gcggtagccg aagccgatcg ggtgcgtgtt gtcgaggtcg cctgcaaaga tggcgccgcc
     4441 gatgagctcg agtgcgtcac gggtttcttt ctcggagtag ggaaagcgat acggctccag
     4501 gatctcatcg gtctcctcgg gatcgtgacc ggagggagtt tcaggagcag cctccggtgc
     4561 gccgggctcc ggaccgagaa cgccttcttc gggatcaatg aaatcaagta cgtttgtcct
     4621 ggcccagaga gccgcctcgc gaagtgcgac gagcgtgccg ccctcggaaa cccattgccg
     4681 aagcctcggc aggaagtcgg gaaggtactc gtcgtacacg ccacctgcga aaacgatgtg
     4741 cgtatagccg ctccagttga tgccgtccaa ctggtcgcgg tcccgcaagg tcacgcgcat
     4801 gcgcatgcga tggtcgagca ggtgccagat ctcgccggcg tcatacaggt ccatgtcacg
     4861 gccgacgacc agcaacacct caggctgttt cagcggcttg aaagaaggcg cgccggcatc
     4921 gatgctctcg cctcctgctg cactggcgcc ggattcgagc gcatgcacgg ggattccgtc
     4981 ctcccgggca atcgcctcca tcacctccct tatttcggca cggcttttct cctggcggtc
     5041 atagggaacc aggatggtgc cccgcgccag ggaaacaggg ccgcgagatg tgcggccggt
     5101 aaacggcttc gtagcgaccc tcgcaagaat ttcttcctcc agtaagcggt tcaatgcgcg
     5161 gggcgcgtaa tagccggacc attcgaatgc ataggcgtag ccggacgtat ccggagtgtc
     5221 ggcaaccggc attgcggctg caacagtctc acccaggagg ttttcgttga aacgacgccc
     5281 cgacagcggc gcgaagtcca ggtcgaaggc aagcggtatg ttccacgtcg agacgtcgta
     5341 aaaagtcgtg tcctcgaact cgctgacggt ctcgaacatg ccgcggatga ggcgaaactg
     5401 cggctggctc accggcacta ccacggcctc gcctggcgag aacgtgacac caccttccgt
     5461 cacttcgcgg ccgaggcgcc aggcgcggat gcgatgggat tgcagcatgt ccaggaagtg
     5521 gaacatccgc gcgggatctc ccggcgctgc aaagacgtac gcctgcacgg ccgcatcgtc
     5581 ggcctcggcg atggcggagt cgtagaattc cttctggtat tggagcagtt cggcacgcag
     5641 gcttgccgcg ctttcgatgc tcgcgaggct ggtgcggaaa tgcttgcgta tgttttcgcg
     5701 gtatgtacgc agtccgttcg cggtgtctat ttccacacca agcgccgcgg ccgcctcgta
     5761 gagaatgccg acgccgccgt tgacgaatgg aaaggtggaa cccttgccta tgtagtagtt
     5821 gtcgaagctt tcgccgtgga agtaaagcct gccctcggaa tccaggaatt cctcggaggt
     5881 acgcacgacg tccgccatca gctgc
//
LOCUS       DASUOM010000056         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_703380,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000056 DASUOM010000000
VERSION     DASUOM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703380"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFY27_01235"
     CDS             complement(<1..893)
                     /locus_tag="VFY27_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764161.1"
                     /translation="MSFLKELERRNVVRVALAYLAAAWLFIEVADTVLPRLGFSDAAV
                     TNVILLLAIGFVPVLVLAWVFEWTPEGMRRDTGETTREAAAPANKRLDRIIMVTLALA
                     VGYFAVDKFVFDPSRDASLRDRAQQEGRTAAYTDAYGERSIAVLPFDNLSSDPEQGFF
                     ADGISEELLNLLARVEGLRVISRTSAFSFKDSNEDLRSIAAKLDVAHILEGSVRRSGN
                     RIRITAQLIDARNDAHLWSETYDRILDDIFDIQDEVAAHVVEQLKLTLALAPPRATRA
                     NLQAYTLYLKAQQMVAAGDPS"
     gene            complement(911..>3580)
                     /locus_tag="VFY27_01240"
     CDS             complement(911..>3580)
                     /locus_tag="VFY27_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764162.1"
                     /translation="EAIAFSGRPTLEDALNQMPQVVPDFNRTANNPGDGTARINLRGF
                     GAGRTLVLLNGRRIAPSGVGGAIDINNLPQALVDRVEIITGGATTVYGSDAVAGVVNF
                     ITRDDFAGLEIDASVNTTEENDANAYDVNVAFGNDFGNGRGNVTLYAGTHDREALFAS
                     ERELTRQSFQNDDTDGTLLEGGNVVTPEGVIFFPEVNLGTNSPWVTFNADGTPRGFVE
                     PDDRYNFQSVNYLQTPLTRYHGGALATFELGSSHELYLETSYSSNEAQQVLAPVPAAG
                     FVNVNIDNLLLTAETRQLFIDNYEIEPGLAEFFVGRRLSDIGPRIIDSERDYWRSVVG
                     LRGDIGNNWDIDAWVTYTRSSETQFLRNDGSASRFQQGLLVDPLTGECFDTSNDCVPI
                     DIFGPGRLSVAAADFLRVTNVQNDTERTQILASLVVSGTPLETRGGPLDTALGIEWRS
                     DDASFEADDVLFTGDTLAYRGEASVDGRESVAEVYAEALVPLAQDKAWAQYFALELGG
                     RYSRYDKAGGVWTYKMGSQWQLSDKLRFRGMHQKSVRAPNNLELFQQPFAESFLLEEL
                     NIEDPCSASNDPVGAGVADKCVLQGVPADQVGIFEAGPVPVDFVQGGNPQLAPEEAKT
                     WTLGVVLTPERFFNWQVAIDWFKLEVTDSIGSIDALAICFDPLNAGNLFCENIRRNTT
                     SGNISEVSQLQSNRGLIGTEGIDTLVNYETGLPAAFSVFEGDARVSIDLTWTHTFKNR
                     WQLNPVTRIVDCVGYFGDFCGLGVTGDFGFTLPENRVTTHVNYVSGPLNVHLTSRWID
                     GTSNSRALEAVFFGSPQPVLAIPAIGSKHYLDLGFGYSFGESITFRAVINNLGDTSPP
                     NMADAVTANNTDSFLYDVFGRSFHLALSARLFR"
BASE COUNT          685 a         1083 c         1123 g          689 t
ORIGIN      
        1 tctgacgggt cgccggctgc caccatctgc tgcgccttga ggtaaagcgt ataggcttgc
       61 aggttggcgc gagtcgcccg cgggggagcg agtgccagtg tcagcttcag ctgctcgacg
      121 acatgtgcgg caacctcatc ctggatgtcg aagatgtcgt ccagtatccg gtcataggtt
      181 tccgaccaga gatgtgcgtc attacgtgcg tcgatcagct gcgccgtgat gcgaatgcga
      241 ttgccggagc gtcgcaccga gccctccagg atgtgcgcga cgtcgagctt cgcggcgatg
      301 cttcgcaggt cctcgttgct gtccttgaag gagaatgcgg acgtgcgcga gatgacccgc
      361 aagccctcga ccctggccag gaggttcaac agctcctcgg agatgccgtc tgcaaaaaag
      421 ccctgctccg ggtcagaact caggttgtcg aacggcagca cggcaatcga cctctctcca
      481 taggcgtcgg tgtaggcggc agtccggcct tcctgttgcg cgcgatcccg caggctggca
      541 tcgcgcgacg ggtcgaaaac gaatttatct acggcaaaat aacccaccgc cagcgccagc
      601 gtaaccatga tgatgcgatc caggcgcttg ttcgcgggcg ccgctgcctc acgggttgtc
      661 tcgccggtgt cgcgccgcat cccctccggc gtccactcga atacccaggc gagtaccagg
      721 acgggcacga agccgatcgc aaggagaagg atgacgttcg tgaccgcagc gtcggaaaag
      781 cccaggcgcg gcaacaccgt gtcagcaacc tcgataaaga gccacgccgc cgccaggtag
      841 gcaagtgcaa cccgaacgac gttgcggcgt tcgagttcct tcaggaatga catgaccgcc
      901 ggctacgatg tcagcggaag agccgcgccg aaagcgctag atgaaaactg cggccgaaga
      961 cgtcgtagag aaacgagtcg gtattatttg ccgttacagc gtccgccatg ttcggcgggc
     1021 tcgtgtcgcc caggttgttg atcaccgccc gaaaggtgat gctctcgccg aagctgtagc
     1081 cgaagccgag gtcgaggtag tgcttgctgc cgatggctgg aatcgccagc accggctggg
     1141 gcgatccgaa aaaaaccgcc tcgagtgcgc gcgagttgga tgtgccgtcg atccatcgcg
     1201 aggtcaggtg gacgttcaag ggaccgctca cgtagttgac atgcgtggtg acgcgattct
     1261 ccggcagggt aaagccgaag tccccggtga ccccgagccc gcagaagtca ccgaagtagc
     1321 caacgcaatc gacgatgcgg gtgacaggat tgagctgcca ccggtttttg aaggtatgcg
     1381 tccaggtcag gtcgatggac acccgcgcat cgccctcgaa cacggaaaat gcagcgggca
     1441 gcccggtttc gtagttaacc agcgtatcga tcccttctgt accgatcaaa cctcgattgc
     1501 tctgcagctg cgacacctcg ctgatgttac cgctcgtcgt attgcggcgg atattctcgc
     1561 agaacaggtt gcccgcgttg agcgggtcga agcagattgc cagtgcgtct atgctgccga
     1621 tgctgtcggt gacttcgagc ttgaaccagt cgatcgcgac ctgccagttg aagaagcgct
     1681 cgggtgtcag cacgactcca agcgtccagg tcttcgcttc ctcgggcgcg agttgcggat
     1741 tgccgccctg cacgaaatct accggcaccg ggcccgcttc gaatataccg acctggtcag
     1801 cgggcacgcc ctggaggacg cacttgtcgg caacgccggc accgaccggg tcgttcgacg
     1861 cggaacaagg gtcttcgata ttgagttcct cgagcaaaaa agactccgca aacggctgct
     1921 ggaacagctc gagattgttc ggagcccgaa cactcttctg gtgcatgccg cggaaacgca
     1981 gcttgtcgga cagctgccat tggctgccca tcttgtaagt ccacacgccg ccggccttgt
     2041 cgtaacggga gtaacgtcca cccagttcga gggcgaagta ctgtgcccat gccttgtcct
     2101 gggcgagcgg caccagcgct tccgcgtaga cctcggcgac gctttcccgc ccatcgacag
     2161 aagcctcacc cctgtaggca agcgtatcgc cggtaaacag gacgtcgtct gcctcgaagc
     2221 tggcatcgtc gctgcgccat tcgatgccga gggcggtatc gagcgggcct ccccgggttt
     2281 cgagcggcgt tcctgacacg accaggctgg cgagtatctg cgtgcgctcc gtatcgttct
     2341 ggacgttggt gactcgcaga aaatcggctg cggccacgga aaggcgaccg ggaccaaaaa
     2401 tgtcgatggg tacgcagtcg ttgctcgtgt cgaagcactc gcccgtgagc gggtcgacca
     2461 gcaggccctg ctggaagcgg ctggccgacc cgtcgttgcg caaaaactgg gtctcgctcg
     2521 acctggtgta ggtcacccag gcatcgatgt cccagttgtt gccgatatcg ccgcgcaggc
     2581 cgacgacgga gcgccagtag tcgcgctcgc tatcgatgat gcgcggcccg atgtcgctca
     2641 gccgccgccc cacgaagaat tcagcgaggc cgggctcgat ttcgtagttg tcgatgaaaa
     2701 gctggcgtgt ctctgccgtg agaagcaggt tgtcgatatt gacgttaaca aatcctgcag
     2761 cgggaaccgg cgccagcact tgttgagctt cattgctgct gtagctggtt tcgaggtaga
     2821 gctcatggct gctgcccaac tcgaacgtgg cgagcgcgcc accgtggtaa cgggtcagcg
     2881 gcgtctgcag gtaattgacg ctctggaaat tgtagcggtc gtcaggttct acgaagcccc
     2941 gcggcgtgcc gtctgcgtta aaggtcaccc aggggctgtt cgtgccgaga ttcacctcag
     3001 gaaaaaagat gaccccttcc ggtgtgacaa cattgccccc ttccaacagc gtaccgtcag
     3061 tgtcgtcgtt ctggaagctc tgccgcgtca gttcccgctc ggacgcgaac agtgcctctc
     3121 gatcatgtgt tcccgcgtac agcgtcacat tgccgcggcc gttgccgaag tcgttgccga
     3181 aggccacatt aacgtcgtag gcattggcat cgttctcttc ggtggtgttg acgctcgcat
     3241 ctatctcgag acctgcaaag tcgtcacggg tgatgaagtt gaccacgccg gcgacggcat
     3301 cggagccgta cacggtggtg gcgccgccgg taatgatctc gacccggtct acgagcgcct
     3361 gcggcaaatt gttgatgtcg atcgcaccgc caacgccgga gggggcgatg cgcctgccat
     3421 tgagcaatac gagcgtccgc cccgcgccga agccgcgcag gttgatcctg gcagtgccat
     3481 cgccggggtt attggcggta cgattgaaat ccggcacgac ctgcggcatc tggttgaggg
     3541 catcctcgag agtcggccgg ccggagaagg cgatcgcctc
//
LOCUS       DASUOM010000057         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1063243,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000057 DASUOM010000000
VERSION     DASUOM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063243"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFY27_01245"
     CDS             <1..250
                     /locus_tag="VFY27_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5764163.1"
                     /translation="ILRADPIRVVEAVVTGTAFLGAGTIFRDRERGVEGLTTAASLLL
                     VGAIGISVALKQLLTAVLVTILTLLLLRAARWFKTAKH"
     gene            complement(356..928)
                     /locus_tag="VFY27_01250"
     CDS             complement(356..928)
                     /locus_tag="VFY27_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5764164.1"
                     /translation="MDKPLKQKTIAFLATDGVEQVEFTKPWDAIKAAGAEVHLISLEP
                     GEVQGFNHLDKGDSFHVDRTVDSAKASQYDGLCIPGGVANPDALRMNGDAVEFVREFF
                     EQSKPVAAICHAPWLLVEADVVQGRRLTSWPSLKTDIENAGGTWVDEEVVVDNGLITS
                     RKPDDLDAFCAKAVEEFSEGRHEQQAASAR"
     gene            complement(988..3492)
                     /gene="ligD"
                     /locus_tag="VFY27_01255"
     CDS             complement(988..3492)
                     /gene="ligD"
                     /locus_tag="VFY27_01255"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5764165.1"
                     /translation="MAKERLSEYNARRNFARSKEPPGKPGRSRRKSLGFFVQKHAATR
                     LHYDLRLEWDGVLLSWAVTRGPSPDPKQKRLAVRTEDHPMSYAQFEGTIPKKEYGGGT
                     VMLWDKGRWVPKEDTDEGLKQGKLKFFVEGERMQGGWTLVRMKPRKRGDRENWLLIKE
                     RDEHAGHDPDELVNAYTTSIATARTMEQIANDEDPPKKTKLGKRRTAKEKTSKKARKL
                     PPFRKPQLATLSDTAPDGDEWVHESKFDGYRSIVAVAGDEVRCYSRAGHDWTDRFARV
                     AEALRDLDCENALLDGEVVAPHTAAGSQFSALQAALSSGGPLAYYAFDLLELDGEDLT
                     SRPLLERKANLRKLLGTLPGRSIVIYSEHVRGSGQEVLDAMCRNGGEGIVSKKVNSVY
                     RGRRTTSWLKIKCTKRQEFVIGGFTPSDKRGREFASLLVGTFENGELQYRGRVGSGFS
                     GTHLEDLARRMQSLQQKQSPFATTPKSVSRNARWIRPEMIAEIDFNELTDGGHVRHGV
                     FKGLREDKKPEQVSLERATGADPKVREEYHGIRISNPHRVIYPKLGITKAELAHYYDA
                     VSGRMLPHVVNHPISLLRCPQGLDDKNRNDGCFFQKHASAGFPDELKRVNVREKSSKR
                     ADYLYIDNVRGLIAAVQMGTLEFHVWGSKIDSLEKPGRVVFDLDPDESLEFPVVALAA
                     KVLKQRLQKLGLKTVALVTGGKGVHVIAPLRRTADWKQVKGFAQGFATQLAAEEPDIF
                     VATMSKSRRRGRIFVDWLRNERGSTAIAPYSTRARDNGPVATPVTWDELEDLSSANCF
                     RTDDVIRRLEDDDPWDEAKALRQSITAQMLERVSIE"
     gene            complement(3492..4379)
                     /locus_tag="VFY27_01260"
     CDS             complement(3492..4379)
                     /locus_tag="VFY27_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5764166.1"
                     /translation="MAARPLWKGQLRLSLVAMAVELYSATKTGARVSFRQIHEPSGKR
                     IRYEKVVPGIGPVEKDDIMKGYELDKDDYVLLDQEEIEAVKLESKKTFELIQFVDGCE
                     IPPLYFDKPYYLVPADDLAEDAYRVIREALRASGKVGLGQITLRGREYIAAVKPCNDG
                     LLLETLHYEDEIREAEPLFSKISDEKVQEDLLDVATTLIEKKTAKFDISRFKDNYTVA
                     MRDLIEQKTKAKGAKRVSTDEDEERPVTGDNVIDLMAALKKSLERTSGEGARSKTGKK
                     TQKKQSKKKKAPSRKRKRA"
     gene            4677..>4989
                     /locus_tag="VFY27_01265"
     CDS             4677..>4989
                     /locus_tag="VFY27_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764167.1"
                     /translation="MITPDVYSASDTDFPRLLLASNREPFRVIRDANGEVSRCDRTMG
                     GLTSALCPVLERCDGVWVSWNPHALDIEDDDHIREYRGDGIPFPVIQVGLRKPEVRGF
                     YN"
BASE COUNT          827 a         1599 c         1443 g         1120 t
ORIGIN      
        1 aatcttgagg gcggatccca tccgcgtcgt agaggccgta gtcaccggca cggcttttct
       61 tggagccggc acgatcttcc gtgaccgcga gcgcggcgtg gagggactca ccacggcggc
      121 atcactgctt cttgtcggcg ccatcggcat ctccgtggcg ctgaagcagt tgttgacggc
      181 cgtcctcgtg acgatcctga ccctcctgct tttgcgcgcc gcacgctggt tcaaaaccgc
      241 gaagcattga aactccgctg ctcggaactg taggagcccg ccatgcgggc gagcgcgatt
      301 acgcgccggc gaactcgccc gcatgtgctt ctaccgggcc cggctcgtca caacgctagc
      361 gcgccgatgc cgcctgttgc tcgtggcggc cttcgctgaa ttcttccacc gccttcgcgc
      421 agaacgcgtc gaggtcatcg ggtttcctgc tcgtgatgag gccgttgtcg acaaccactt
      481 cctcgtcaac ccatgtgccg ccggcgtttt cgatatctgt cttcaagctg ggccaggatg
      541 tgagcctgcg cccctgcacg acatcggctt caacaagtaa ccatggcgca tggcagatcg
      601 ccgctaccgg cttcgactgt tcgaagaatt ccctgacgaa ctcgaccgcg tccccgttca
      661 tccgcaaagc gtccggattg gcgacgccgc cgggaatgca gaggccgtcg tactgcgagg
      721 ccttggcgct gtcgactgtc cgatcgacat ggaaactgtc gcccttgtcg aggtgattga
      781 agccctgtac ctcccccggc tccagcgaga tcaggtgaac ctcggcaccc gcggccttga
      841 tggcgtccca cggctttgta aattcaacct gttccacgcc gtccgttgca agaaaggcaa
      901 tggttttttg cttgagcggt ttgtccatga tgatccctcg gtatgcttaa gaagaacgcc
      961 tcccatcaag caaaagctgt gccaccgtca ttcgattgaa acgcgctcca gcatctgcgc
     1021 cgtgatcgac tggcgcaacg ccttcgcttc gtcccaggga tcgtcatctt ccaggcgccg
     1081 gatgacatca tcggtgcgaa agcagttcgc cgacgaaagg tcttcgagtt catcccaggt
     1141 taccggcgtc gcgacgggac cgttgtcgcg cgcgcgggtg gagtagggcg caatcgccgt
     1201 cgagccgcgt tcgttcctga gccagtcgac gaaaatccgc ccgcgccgcc ggctcttgga
     1261 catcgtcgcg acgaagatat ccggctcctc ggcggcgagc tgcgttgcaa agccctgcgc
     1321 gaaacccttg acctgtttcc agtccgcagt gcggcgcaga ggtgcgatga catgcacgcc
     1381 cttgcctccc gtcaccagcg ccacggtctt caggccaagc ttttgaaggc gctgcttcaa
     1441 taccttcgca gcgagagcca ccaccgggaa ttccagcgac tcatcggggt cgaggtcgaa
     1501 caccacccgg ccgggctttt ccagggaatc tatcttcgat ccccaaacgt ggaattccag
     1561 cgttcccatc tgcaccgcgg caatcaaccc cctaacgttg tctatgtaga gatagtccgc
     1621 gcgcttgctc gatttctccc ggacgtttac gcgcttcagc tcgtccggaa atccggccga
     1681 tgcgtgtttc tggaagaagc agccgtcgtt gcggttcttg tcgtcgagtc cctgggggca
     1741 ccgcagcagc gagatcgggt gattcaccac gtgcgggagc atgcgcccgg aaacggcgtc
     1801 gtaatagtgt gcaagttccg ctttcgttat accgagcttc gggtagatga cccggtgcgg
     1861 gttggagatg cgaatgccgt gatactcttc cctcaccttc gggtctgctc ctgttgcacg
     1921 ctcgagagac acctgctccg gcttcttgtc ctcgcgcagg cctttgaaaa cgccatgacg
     1981 cacatggccg ccgtccgtca gctcgttgaa atctatctcg gcgatcatct ccgggcgtat
     2041 ccaccgggcg ttgcgcgaaa cggatttcgg cgtcgtggcg aaaggcgact gcttttgctg
     2101 cagcgactgc atccgcctgg cgagatcctc gagatgcgtg cccgagaaac cgctgccgac
     2161 gcggccgcga tattgcaatt caccgttctc gaaggtgcct accagcagcg atgcgaattc
     2221 ccgcccgcgc ttgtcggacg gggtgaatcc gccaattaca aactcctggc gcttcgtgca
     2281 cttgatcttg agccagctcg tggtacgccg tccgcggtaa actgaattca ctttcttgga
     2341 gactatccct tctccgccgt tcctgcacat cgcgtccagg acttcctggc cgctgccgcg
     2401 tacgtgctcg ctgtagatga cgatcgagcg gccggggagg gtgccgagca atttgcgcag
     2461 gtttgccttg cgctcgagca gtggccgcga cgtgagatcc tcgccgtcga gttcgagcag
     2521 atcgaacgcg tagtaggcga gggggccgcc cgaggacaag gcagcctgta acgccgagaa
     2581 ctgcgatccg gccgcagtgt ggggcgcgac aacctcgcca tcgagcagtg cgttctcgca
     2641 atcgaggtcg cgcagtgctt ccgccacgcg cgcgaacctg tccgtccagt cgtgccccgc
     2701 tcgcgaatag cagcgaactt catcgccggc aacggccacg atagagcgat agccgtcgaa
     2761 cttggattcg tgcacccact catcgccgtc aggagccgtg tctgacagcg tcgcgagctg
     2821 gggcttgcgg aacggcggca gcttgcgcgc ctttttcgaa gttttctcct tcgctgtgcg
     2881 acgcttgccg agcttcgtct ttttcggcgg atcctcgtcg ttcgcgatct gctccatcgt
     2941 gcgcgccgtg gcgatgctgg tggtataggc gttcaccagt tcgtcggggt cgtgtccggc
     3001 atgctcatcc cgttccttga tcagcagcca gttctcgcgg tctccgcgtt tgcgcggctt
     3061 catgcgaacg agggtccagc ctccttgcat ccgttcgcct tcgacgaaga acttgagctt
     3121 gccctgcttc aggccttcat cggtgtcttc cttcggcacc cagcggccct tgtcccacag
     3181 catcaccgtg ccgccgccat actctttctt cgggatggtc ccttcgaact gtgcgtacga
     3241 catcgggtgg tcttccgtgc gcacggccag tcgcttttgc ttcggatcgg ggcttggccc
     3301 acgcgttacc gcccagctga gcagcacgcc gtcccactcg agacgcaagt cgtagtgcag
     3361 gcgggttgcg gcgtgcttct gaacgaagaa gcccagggac ttgcgccgcg agcgaccggg
     3421 ctttcccggc ggttccttcg acctggcaaa attccgcctg gcgttgtact ccgagagccg
     3481 ctctttcgcc actaagcccg cttccttttc ctgctcggcg ccttcttttt cttcgactgc
     3541 ttcttttgcg tcttctttcc ggttttagac ctggcgcctt cacccgacgt cctttccagg
     3601 cttttcttga gcgccgccat cagatcgatc acgttgtcgc cggtaaccgg ccgctcctcg
     3661 tcttcatccg tcgagacccg tttggcgccc ttggctttcg tcttttgctc gatcaagtca
     3721 cgcattgcca ccgtgtagtt gtccttgaac cgcgagatgt cgaatttcgc agtcttcttt
     3781 tcgatgaggg tggtcgccac atccagcagg tcctcctgca ctttttcgtc gctgatcttc
     3841 gagaacagcg gctccgcctc gcggatctcg tcctcgtagt gcagggtctc gagcagcaag
     3901 ccgtcattgc agggcttcac ggcggcgatg tattccctgc cgcgtaaggt gatctgaccc
     3961 agcccgactt tgccggaggc gcgaagcgct tcgcggatta cgcgataggc atcctccgca
     4021 aggtcgtctg ccggcaccag gtagtagggc ttgtcgaagt agagcggtgg aatttcgcag
     4081 ccatcgacaa actggataag ctcgaacgtc ttcttcgact cgagcttcac cgcctctatt
     4141 tcttcctggt cgaggagaac gtagtcgtcc ttgtcgagct cgtagccctt catgatgtcg
     4201 tccttctcca ccggcccgat tcccggcacg accttctcat agcggattct cttcccgctt
     4261 ggttcgtgga tctggcggaa gctcacgcgc gcgccggtct tcgtggccga gtacagttcc
     4321 accgccatcg caaccagcga aagcctgagc tgtcccttcc agagaggtcg tgctgccatc
     4381 ttgttaatcc tttgtaaggg cgcgacctcc gtggctttgc aatatgtatg ccagtcgccg
     4441 ccgccgcgcc cggaggtcgt tcgcgggaag aaatcatcaa gcaaccggat gtcccgacga
     4501 gcgtggagcg agaccggcag cggcgttgta agaatttccg gtatggcggc gatttttcat
     4561 cggcgcgcgt gagcaccggc tttttcctgc gggtttgagc tgcatccgtc cgtggcatac
     4621 atcttgcagc cgctgtcacc gtaatccggc acccgccgtt cccgagcacc gagatcatga
     4681 tcacaccgga tgtttacagc gctagcgaca ccgattttcc gcggttactt ttggccagca
     4741 accgggaacc attcagagtc atcagggatg ccaacgggga ggtcagtcgc tgcgatcgca
     4801 cgatgggagg tctcacctcg gcgctgtgcc cggtactcga acgctgcgat ggcgtgtggg
     4861 tttcctggaa tccgcatgcc ctggacattg aggacgacga tcacattcgc gaatacaggg
     4921 gcgacggtat ccccttccct gtcatccagg taggactgcg aaagccggaa gtccgtggct
     4981 tctacaatg
//
LOCUS       DASUOM010000058         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1300272,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000058 DASUOM010000000
VERSION     DASUOM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300272"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFY27_01270"
     CDS             complement(<1..581)
                     /locus_tag="VFY27_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX5764168.1"
                     /translation="MGTDGFEFVEYAAPDPELLRKLFSRMGLPAVAHHRSKDVTLHKQ
                     RDINFIINAEPDSFAQEFARVHGPCACAMAFRVKDANYALNRAVDLGAKPVPGDPGPM
                     ELNIPAIEGIGGSRLYLVDRYGDQSIYDIDFRYESGWQEQVRELDTGLTYIDHLTHNV
                     HKGQMKVWAEFYETLFNFREIRYFDIEGKHTGL"
     gene            complement(774..1880)
                     /locus_tag="VFY27_01275"
     CDS             complement(774..1880)
                     /locus_tag="VFY27_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX5764169.1"
                     /translation="MTNVKARSPHWWAGVGLAVAVSLAAGISAEWLGETLLGFEKSPI
                     SPIVMAIVIGMIVANSLSVSESVTDGLRFCASTILRIGIMLLGIRLSLLGAGRFTLVA
                     LPFVIVAITIGLTIVGLLGRSLGLSRQLTGLIAVGTSICGCTAIVATAPLIKANEAEV
                     SYSIACITLFGLAVMFVYPVLGHFFFPDQPALAGLFLGTSIHETAQVAGAGLMYQTQY
                     NAPVALDIATVTKLVRNLCMVAVIPLVGILYAGDRGQATGKTRWASIIPWFIVGFALM
                     SALRTVGDLGPRPFGILEPGQWRVMVEFLQVGAERFLLVAMAAVGLTSIFAGLRSIGI
                     RPFLLGLFAAALIGGGSLLLISLFAERLIELAGI"
     gene            complement(1923..>2346)
                     /locus_tag="VFY27_01280"
     CDS             complement(1923..>2346)
                     /locus_tag="VFY27_01280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5764170.1"
                     /translation="MVEEWGGPFAIKGVMSVDDAKRAVEIGASAIMISNHGGRQLDGS
                     RAPFDQLKAIVDAVGGQIEIILDGGVRRGTHVLKALALGATACSGGRWYLYALAAAGT
                     AGVERAMTLLRAEIERDMLLMGCRSIADLDRSKIAPRP"
BASE COUNT          488 a          731 c          686 g          441 t
ORIGIN      
        1 aacagcccgg tgtgcttgcc ttcgatgtcg aagtagcgga tttcgcgaaa attgaacagg
       61 gtttcgtaga actcggccca gaccttcatc tgtcctttgt gaacgttgtg agtgaggtgg
      121 tcgatgtacg tgaggccggt atcgagttcc cggacctgtt cctgccaccc ggattcataa
      181 cggaagtcga tgtcgtaaat ggattgatca ccgtagcgat cgaccaggta gagcctgctc
      241 ccgcctatgc cttcgatcgc gggaatgttc agctccatcg gacccgggtc gccaggcacc
      301 ggtttcgcgc cgagatccac ggcgcggttc agcgcgtagt tggcgtcctt cacgcgaaaa
      361 gccatcgcgc acgcgcaggg gccgtgcaca cgcgcgaact cctgcgcgaa actatccggt
      421 tccgcgttga tgataaaatt gatatcgcgc tgcttgtgca gcgtcacgtc cttggagcgg
      481 tgatgagcga ccgcgggaag gcccatcctg gaaaacagct tgcgcaacag ttcggggtcc
      541 ggcgccgcat attccacgaa ttcgaatccg tccgttccca tcggattcct gctttctccg
      601 acgtgaccag ctgttttctt cgtcgtcctt accaccattg gaaccttctc tgcatcctgg
      661 ggctttttac cgatgccgct cgaatggcgg cagtgcgcct gccgaatttt accgcaacgg
      721 gctttttctt tcccggttga ataccgtcag tggctgcctc ggcccgcagg gcatcaaata
      781 ccggctagtt cgatcagcct ttcggcgaaa agagagatca gcagcaggct cccgccgcca
      841 atgagggcag cggcaaacag gccgagcaga aaaggtctga tgccgatgct gcgcaagccc
      901 gcaaagatgg aagtcaggcc aaccgccgcc atggcaacca gcaggaaacg ctccgcgcca
      961 acctgcagga actcgaccat cactcgccac tggcccggct ccagtatgcc gaacggtcgc
     1021 ggaccgagat cgccgacggt tcggagagcc gacatcagcg cgaagccgac gatgaaccac
     1081 ggaatgatcg aggcccagcg cgtcttgccg gtggcctggc cccggtcgcc agcatagagg
     1141 atgccaacaa gcggaatcac tgcgaccatg caaaggttgc gtaccagctt ggtgacggtc
     1201 gcgatgtcca gtgcgaccgg cgcattgtat tgcgtctggt acatgagacc tgcgccggcg
     1261 acctgtgcgg tctcatggat ggaggtgccg agaaaaagcc ctgccagcgc cggctggtcg
     1321 ggaaaaaaga agtgacccag aaccggatag acgaacatca cggccagtcc gaacaacgtg
     1381 atgcaggcaa tggagtaact gacttccgcc tcgttcgcct tgatcagcgg tgccgtcgcc
     1441 actatcgcgg tgcagccgca aatgctcgtg ccgaccgcaa tcagccccgt cagctggcgc
     1501 gacagcccga gcgaacggcc gagaagaccc acgatggtca gcccgatcgt gattgcgacg
     1561 atcacgaacg gcagcgcgac cagcgtgaac ctcccggcac caagaaggct caagcgaatg
     1621 ccgaggagca tgatcccgat tctaagtatc gtgctggcac agaatcggag gccgtctgtg
     1681 acggactccg acaccgaaag cgaattcgcg acgatcatgc cgatcacgat agccatgacg
     1741 atcgggctga tcgggctttt ctcgaagccg agcagcgtct ctcccagcca ttccgccgat
     1801 atgccggcag caagggaaac ggccaccgca agaccgacac cggcccacca gtgtgggctt
     1861 cgagctttta cgttcgtcac gagggctgtt ccgtttcttc ttgtcttgtc ggggaacgtt
     1921 atctacggcc gcggggcgat ttttgagcga tcgaggtctg caatggaacg gcaccccatc
     1981 agcagcatgt cgcgctcgat ctccgcccgg agcaacgtca tggcgcgttc gactccggcc
     2041 gtcccggcag cggcgagagc atacaggtac cagcgtcctc cggaacaggc agtagccccg
     2101 agagcgagcg ctttcagaac atgcgtgccg cggcgaacgc cgccatccag gatgatttcg
     2161 atctgcccac caacggcatc gacgatagcc ttcaattggt cgaacggcgc ccgggaaccg
     2221 tcgagctgcc ggcctccgtg gttggaaatc atgatcgcgc ttgcaccgat ttccacagcc
     2281 cttttcgcat cgtcaacgga catcacgccc ttgatggcga acggtccgcc ccactcttct
     2341 accatt
//
LOCUS       DASUOM010000059         7854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1426223,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000059 DASUOM010000000
VERSION     DASUOM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426223"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /gene="kaiC"
                     /locus_tag="VFY27_01285"
     CDS             <1..1348
                     /gene="kaiC"
                     /locus_tag="VFY27_01285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX5764171.1"
                     /translation="IGAKRVAIDTIETLFGGLSNQAILRAELRRLFRWLKDKGVTAII
                     TGERGEGQLTRQGLEEYVSDCVILLDHRATEQVSTRRLRIVKYRGTAHGTNEYPFLID
                     EDGITVVPITSLEVRYDVSEEQISTGISALDEMFSGGGYYRGSTILVSGSAGTGKTSV
                     AAHLALATCRRGEKCLYFAFEEAEQQFLRNMRSIGIDLAPFIRQGVFKFQASRPALQG
                     LEQHLVQVHKLVRREQPSVVIIDPISNFVAAGSLSDASAMLTRLIDFLKMNGITAMLT
                     NLTSGSQLEKTDVKISSIVDSWLLLRNIEQDGERNRAMFVLKSRGMAHSNQLREFVMT
                     DRGIDLVDVYIGSEGVLTGSARALQEARDHSEKLVKRQDVERRQRVLDRKRRALESRI
                     AALRAEFETEEEDLKQLLEADEGRERSIADSRTDMGRRRRSAQGQRGHREALNDDE"
     gene            1341..1682
                     /locus_tag="VFY27_01290"
     CDS             1341..1682
                     /locus_tag="VFY27_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5764172.1"
                     /translation="MSDSRSTGGSLDEYPGEPSADVLWEFKLYVAGHTPRSVTAIANL
                     KALCEEYMPGRYRIEIIDLIERPELARSDQIVAIPTLVRKLPGPIRRIIGDLSNTDRV
                     LVGLQVKVPGR"
     gene            1705..3696
                     /locus_tag="VFY27_01295"
     CDS             1705..3696
                     /locus_tag="VFY27_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764173.1"
                     /translation="MISKTEALQQEVQHLRQELQEAEETIRAIQEGRVDAFVVARNAA
                     ERVYTLESGSGPYRILVERMQQCAVTMAPDSTVLYVNCQSSTLVDRSPAELVGSPFRS
                     LVAEADLGQFDAVIKGDADSLQQGEIRLKRPDGTLVPVYLSAGTLSPEEGGGVCVLIT
                     DLTRQKQHEELIAAENLARSMLQQAADAVVVCDPAGVIAFASPAAHELCGRNPLGQPF
                     ADMFPLAITGKTPLAADGYFDLRRLNESIRGLEVTSLRQGSSTRSLMLSAGRVTDSNG
                     EQLGSVVTLADITALKRAERELKEADRQKDEFLAVLAHELRNPLAPIVTGLDVIGLGS
                     DVGEKTAPVLRMIKRQVNHLTRLVDDLLEISRITLGKIELQKEVLEFSDVLSNALDTT
                     REFIESSKHTVHVDLPPEPLRIEADGVRLSQVIVNLLNNASKYTAPGGDILLTARVQE
                     DQLEIRVRDSGRGIAAESLDEVFKMFAQPRRSRPQDGLGVGLALVRRIVELHGGTVGA
                     ASGGLGKGSEFTVRLPLGNTAEEGNKNRSAPEDAGIGGKRVLVVDDNQDSANSLGMLL
                     DILDYEVKVAFDGESALAELDRFSADTIFLDLDMPRMDGFELARRVRQHAEGRNATLI
                     ALTGWGQEDDKKLTRDAGFDHHLLKPVNVDVLRSVLADR"
     gene            complement(3715..4629)
                     /locus_tag="VFY27_01300"
     CDS             complement(3715..4629)
                     /locus_tag="VFY27_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX5764174.1"
                     /translation="MRYPTVKQLRYFLALCDSGHFGRAAQASCVSQSAFSTAIQDLET
                     LLGTQLVDRTNRQVTITAAGQEVATQARLCMRDIEDLMQIAGQRQKPLSGDLRLGVIP
                     TIAPFLLPRVLPALRRAYPSLRLYLTEDQTQRIHEKLLAGDLDLLLLALPFDLRSADE
                     LPLFKDRFRLACREGTERVDPEQYRFNRLDAESILLLEDGHCLRDHALAACRLRDTEK
                     VSRFAASSVLTLVEMVDADLGISFLPEMAEKSALLRNTRVKLYPLKDDSYRTIGLAWR
                     KGSGRVEEFRLLGDFLTEHGSARTAKSS"
     gene            4773..5534
                     /locus_tag="VFY27_01305"
     CDS             4773..5534
                     /locus_tag="VFY27_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX5764175.1"
                     /translation="MFENREGKRVPDVKFRTRQESEWVDRTSGDVFAGKTVVVFSLPG
                     AFTPTCSSSHVPRFNKLAPLFKSHGVDDIVCISVNDAFVMNEWKESQRADNITFLPDG
                     NGHFTEAMGLLVGKEDLGFGKRSWRYSMLVRDGVIEKMFIEPEEPGDPYEVSDADTML
                     RYLAPDDAAPLDVTVFTRNGCPHCARAKGLLRDAGIEFEELRLNREYTDQTLRAVAAA
                     TSVPQVFIGGQHIGGADELEKWLSKNERIVRKAAA"
     gene            5718..7151
                     /locus_tag="VFY27_01310"
     CDS             5718..7151
                     /locus_tag="VFY27_01310"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5764176.1"
                     /translation="MKTKAYVDGNWVDSDSGETYEVTNPANGEVIARVAKCGAAETRR
                     AIEAAARAMDGWRSKSAKQRAAIMRKWFNLMMENQEDLAQLMTAEQGKPLAESRGEIA
                     YGASYIEWFAEEAKRIYGDTIPAPASDKRIVVIKQPVGVVACITPWNFPNAMLARKIA
                     PALATGCTVVCKPANYTPLSAFAFAELGERAGIPAGVINLVSGKTAEIGEELTSNPVV
                     RKLTFTGSTPVGKKLMAACAATMKRTSMELGGNAPFIVFDDADVDEAVKGAIICKFRN
                     AGQTCVCANRILVQDSVYDEFSEKLMAAATELKVGDGMTEGVSIGPLISEEAANDVLG
                     FIDDAVQRGAKVAAGGGRNQLGPNFVNPTILTNVNDEMRVFREEIFGPVAPLFRFKTE
                     DEAIKMANDTAVGLACYFYSRDVGRVWRVAEGLEYGIVGINEGIISNEMAPFGGVKES
                     GQGREGSKYGLDDYLEIKYLCMGGLDD"
     gene            7218..>7854
                     /locus_tag="VFY27_01315"
     CDS             7218..>7854
                     /locus_tag="VFY27_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX5764177.1"
                     /translation="MLQNEIYERARLARDPRFDGQFYIAVKTTGIYCRPICPVMSPKR
                     ENITYFPSAAAASEAGYRPCLRCRPECAPGTPAWGGTSTTVRRGLRLIAGGALDEGNV
                     ELLADRLGVTSRHLRRLFTRHLGASPLAVAHTQRLHFAKRLIDDTALPMTHISAAAGY
                     RSVRRFNDAFIKTYGRSPRELRKVGVNGSGARKSAALVLRLPYRAPFDWAAM"
BASE COUNT         1608 a         2303 c         2516 g         1427 t
ORIGIN      
        1 catcggtgca aaacgcgtcg ccatcgacac catcgaaaca cttttcggcg gcttgtccaa
       61 tcaggcgatc ctgcgcgcgg aactgcgacg tctcttccgc tggctcaagg acaaaggcgt
      121 cacggccatc atcaccggcg agcgcggcga gggccagctt acgcgccagg ggctggagga
      181 atacgtttcg gactgcgtca tcctgctgga ccatcgtgcg accgaacagg tgtccactcg
      241 acgactccgc atcgtcaagt atcgcggcac cgctcacggc accaatgagt acccgttcct
      301 catcgacgag gatggcatta cggtggtgcc catcacctcg ctcgaagtga gatatgacgt
      361 ttcagaggag cagatttcca ccggcataag cgcgctggac gagatgtttt cgggcggcgg
      421 ttattaccgc ggcagcacca tcctcgtgag cggcagcgcc ggcaccggga agacgagcgt
      481 ggcggcccat ctcgccctgg ccacctgcag gcgcggtgag aagtgcctgt acttcgcctt
      541 tgaggaggcg gagcagcaat tcctgcgcaa catgcgctcg atcggcatcg atctcgcacc
      601 cttcatcagg cagggtgttt tcaagttcca ggcaagccgt ccggctcttc agggactgga
      661 gcagcatctc gtgcaggtgc acaagctggt gcggcgtgag caaccctcgg tcgtcatcat
      721 cgaccctatc agtaacttcg tcgcggcggg atcgttgtct gacgcgagcg cgatgctgac
      781 aaggcttatc gattttctga agatgaacgg cattacggcg atgctgacca acctgacgtc
      841 gggatcgcag ctggaaaaaa cggatgtaaa gatctcgtcc atcgtggaca gttggctgct
      901 gttgcgcaac atcgaacagg atggcgaacg aaaccgcgcc atgttcgtgc tcaagtcccg
      961 cggaatggcg cattcaaatc agcttcggga gttcgtcatg accgatcgcg ggatcgacct
     1021 tgtggacgtc tacatcggtt cggagggggt gctcaccggc tccgcccgag ctctgcagga
     1081 ggcacgtgac cactctgaaa agctcgtgaa acgccaggat gtcgagcgcc gtcaacgcgt
     1141 gctggaccgc aagcggcgcg cgctcgaatc ccggatcgca gcattgcggg cagagttcga
     1201 aacggaggaa gaagacctga agcagttgct cgaagcggac gagggccggg agcggtcgat
     1261 tgccgacagc cgaaccgaca tgggccggcg ccgcagaagc gctcagggac agcgaggtca
     1321 ccgggaagct ctcaatgacg atgagtgaca gcaggtcgac tggcggttca ctggatgaat
     1381 atcccgggga gccctccgcc gacgtcctct gggaattcaa gctgtatgtc gcaggtcaca
     1441 cgccccgcag cgtgaccgcc atcgccaacc tgaaggcgct ttgcgaggaa tatatgccag
     1501 gacgctaccg tatcgagatc atcgatttga tcgagcgtcc cgagctcgcc cgaagcgacc
     1561 agatcgtcgc catcccgaca ttggtccgca agctgccggg gccgattcgg cgcatcatcg
     1621 gcgatctctc caacacggat cgcgtgctgg tcggcctgca agtaaaggtg cctggcagat
     1681 gatgatgttg aaggacgccg gcggatgatt tcaaagacag aggcgctgca acaggaagtc
     1741 cagcacttgc ggcaagagct gcaggaggcg gaggaaacca tacgcgcgat ccaggagggg
     1801 cgggtcgatg ccttcgtggt cgctcgaaac gcagcggaac gcgtctacac gctggaaagc
     1861 ggttcgggtc cctaccgaat tcttgtggag cggatgcagc aatgcgcggt gaccatggcg
     1921 cccgacagca ccgtcctcta tgtcaattgc cagtcctcga cgcttgtcga ccgttctccg
     1981 gcggaactcg tcggttcgcc attccggtca ctggttgccg aggcggacct cgggcaattc
     2041 gatgcagtga tcaaaggcga cgcagattcg ctgcagcagg gagagattcg gctcaagcgc
     2101 cctgacggca cgctggtccc ggtgtacctg agtgccggga ccctgtcgcc ggaagaaggc
     2161 ggcggcgtat gcgtgctcat cacggatctc acgcgacaga agcagcacga ggagctgata
     2221 gcggcagaga acctcgcacg gtcgatgctg cagcaggccg ccgacgccgt ggtggtgtgc
     2281 gatcccgccg gggtcatcgc gtttgccagc cccgcggcgc atgagctgtg cggccgtaat
     2341 ccgctggggc agccatttgc ggacatgttt ccgctcgcga ttacaggaaa gacgccgctg
     2401 gcagcggatg gttactttga cctgagacgg ctcaacgagt ccatccgcgg cctcgaagtg
     2461 acgtcgctgc ggcagggctc ttccacaagg agcctgatgc tgagtgcggg gcgtgtgaca
     2521 gacagtaacg gggagcagct tggatccgtt gttacgcttg ccgacatcac tgctctcaag
     2581 cgcgccgaac gcgagctgaa agaggccgac cggcagaagg acgaattcct ggctgtactg
     2641 gctcacgaac tgcgaaaccc gttggcgccg atcgtcaccg ggctggatgt cataggactc
     2701 ggcagcgacg tgggagaaaa gactgcgccg gtattgcgca tgatcaagcg ccaggtaaat
     2761 cacctgacgc gtctcgtgga cgacctgctg gaaatctcga ggatcacgct cggcaagatc
     2821 gagttgcaga aagaagtgct cgagttctct gacgtcctgt cgaatgcgct cgatacaacc
     2881 cgcgaattca tcgagtcttc gaaacatact gtccacgtgg atctgccgcc ggaacctctt
     2941 cgcatagaag ccgatggggt tcgcctgtcg caggtaatcg tgaacctgtt gaacaacgcc
     3001 tcgaagtaca cggcccctgg cggcgatatc cttctgacgg cgagggtgca ggaggatcaa
     3061 ctggagatac gggtgcgtga cagcggtcgc ggcattgcag ccgagtccct ggatgaggtg
     3121 ttcaagatgt tcgcccaacc gcgccgctca aggccccagg acgggctggg agtgggttta
     3181 gccctggtga ggcgaatcgt tgagctgcat ggcggaacgg tgggtgccgc aagcggcggt
     3241 ctcggtaagg gaagtgagtt caccgtgcgg ctgccgctcg gaaatacagc ggaagaggga
     3301 aacaagaatc ggtcagcgcc tgaagatgca gggattggcg gaaagcgcgt gctggtagtt
     3361 gacgacaatc aggattcggc gaacagcctc ggcatgttgc tggatatcct ggactatgaa
     3421 gtgaaagtag cttttgatgg cgaatcggcc ctcgcggagt tggatcgctt tagcgccgac
     3481 acgattttcc tggacctgga catgccgcgc atggatggat tcgaactcgc gcgccgcgtt
     3541 cgtcagcacg cggaaggcag gaatgccact ttgatagcct tgaccggatg ggggcaggag
     3601 gacgacaaga aactgacacg ggacgcaggc ttcgaccatc acctgttgaa gccggtcaac
     3661 gtagacgtcc tgcgatccgt gctggcggac cggtaagtca ggggaggctt cgatctagct
     3721 gcttttcgcc gtccgcgcgc tgccatgctc cgttaaaaag tcgcccagca gccggaactc
     3781 ctccacgcgg ccgctgccct tgcgccaggc caggccgatc gtgcgatagc tgtcgtcttt
     3841 cagcggataa agcttcacac gcgtattccg caacagcgcg gatttttcgg ccatctctgg
     3901 caaaaagctt attccaaggt cggcatccac catttccacg agggtaagca cgctgcttgc
     3961 tgcgaagcgg ctgacctttt cggtgtcacg cagcctgcag gcggcgagtg cgtggtccct
     4021 gaggcagtgg ccatcctcga gcagcagaat gctctccgcg tcgagtcgat tgaagcgata
     4081 ctgctccggg tctacccgtt ccgtgccctc ccggcaggcg agccggaagc ggtccttgaa
     4141 gagcggcagt tcatccgcgc tacgcaggtc gaacgggagc gccagcagca ggaggtcgag
     4201 gtctcctgca agcaacttct cgtgaatgcg ctgcgtctgg tcctcggtga gatatagccg
     4261 cagcgaggga taggcgcggc gcagcgccgg cagcacgcgc ggcaacagga aaggcgcgat
     4321 ggtcgggatg acgccgagcc gcaggtcgcc ggatagcggc ttttgccgct ggcccgcgat
     4381 ttgcatcagg tcttctatgt cacgcatgca gagccgtgcc tgggtcgcca cctcctggcc
     4441 ggccgccgtg atcgtgacct gccggttcgt gcgatccacc agctgcgtac caagcagcgt
     4501 ttccaggtcc tggatggcgg tgctgaaggc ggattgcgag acgcacgaag cctgcgcagc
     4561 acgtccgaaa tgtccggagt cgcacaaggc gaggaagtag cgcagctgtt tgacggtagg
     4621 atatcgcatg atcggtcaaa gcggacgtag cgatcatatt aatccatttc ccatatggat
     4681 taacaggggc tagagtggct gcagctggcc caaagaacct acagcaattc ttcgcataat
     4741 ctaagcttta cattacctca ggagagaaat cagtgtttga aaatcgagaa gggaagaggg
     4801 taccggacgt caagtttcgc acgcgacagg agagcgaatg ggtcgaccgg accagcggcg
     4861 acgtgttcgc cgggaaaacg gtcgtggtct tctccttacc aggggcgttc accccgacct
     4921 gttcttcgtc gcacgtgccg cggttcaaca agctggcgcc gctgttcaaa tcccacggcg
     4981 tcgacgatat cgtgtgcata tccgtcaacg atgcatttgt catgaacgag tggaaggaaa
     5041 gccagagggc ggacaacatt acgttcctgc ctgatggcaa tggccatttc actgaagcga
     5101 tgggcctgct cgtaggcaag gaagatctgg gcttcggcaa gcgttcctgg cgctactcga
     5161 tgctggtgcg cgatggcgtc atcgagaaga tgttcatcga gccggaagag ccgggcgatc
     5221 cgtacgaagt atccgacgcc gacacgatgt tgcgttacct ggcgcccgac gatgccgcac
     5281 cgctggacgt cacggtgttc acccgcaacg gctgcccgca ttgcgcaagg gccaagggtc
     5341 tcctgcgcga cgccggaatc gagttcgagg agcttcggct gaaccgtgaa tacacggacc
     5401 agacgctgcg cgctgttgca gcggcaactt ccgttccgca ggtgtttatc ggcggccagc
     5461 acatcggcgg tgccgatgaa ctcgaaaagt ggctctcgaa gaacgagaga atcgtccgca
     5521 aggcagcggc gtaagtttta caagaagtcg caaggcagca gcgggacgcg tttggcgtcc
     5581 cgctgctttt ttttgccgct gcaaagctgc cgcccgcctg cgattgtggt gccggccgtt
     5641 tgcctctatt ctcgtattcc gcaacgagat gagaatggag ccaaatggtg agtgtcagcg
     5701 tatccgataa agcgctattg aaaacgaagg cttacgtaga cggcaactgg gtcgacagcg
     5761 acagcggcga gacctacgag gtgacgaatc cggcaaacgg ggaagtcatc gcccgggttg
     5821 ccaaatgcgg tgcggcggaa acccgccgtg cgatcgaagc ggcggcgcgc gcgatggatg
     5881 gctggcgcag caagagcgcg aagcaacgcg ccgcaatcat gcgcaagtgg ttcaacctga
     5941 tgatggagaa ccaggaagac ctggcgcagc tgatgaccgc ggagcagggc aagccccttg
     6001 cggagtcgcg gggcgaaatc gcctatggcg ccagttatat agagtggttc gccgaagagg
     6061 ccaagcgcat ctacggcgat accatacctg cgcccgccag cgacaagcgc atcgtcgtca
     6121 tcaagcagcc ggtcggcgtc gttgcctgca ttaccccctg gaatttcccg aacgcgatgc
     6181 ttgcgcgcaa gatcgctccc gcgcttgcaa ccgggtgcac ggtcgtttgc aaaccggcga
     6241 actacacgcc gctctccgcg ttcgcattcg cggaactggg cgaacgcgcc ggaattcccg
     6301 ccggcgtcat caacctggtt tcgggcaaaa cggcggaaat cggcgaggaa ctgacctcca
     6361 accccgttgt ccgcaagctg acgtttaccg ggtcgactcc cgtgggcaag aaactgatgg
     6421 ccgcgtgcgc ggccaccatg aagcgcacgt cgatggagct cggcggcaac gcccctttca
     6481 tcgtcttcga cgatgcggac gtggacgagg ccgtgaaggg cgcgattatc tgcaagttcc
     6541 gcaatgccgg gcagacctgc gtctgcgcga accgtattct ggtgcaggac agcgtctacg
     6601 atgagttcag cgaaaagctg atggccgccg cgaccgaact caaggtcggg gacggcatga
     6661 ccgagggcgt gagcataggg cctctcatca gcgaggaagc cgcgaacgat gtgctcggct
     6721 tcatagacga cgcggtgcaa cgtggggcga aggtcgcggc cggtggcggg cgtaaccagc
     6781 tcggtcccaa tttcgtgaac ccgaccatat tgaccaacgt caacgacgag atgcgcgtgt
     6841 ttcgggagga aatattcggg ccggtcgcgc cgctgttccg gttcaagact gaggatgaag
     6901 ccatcaagat ggcgaacgac accgcggtcg gccttgcctg ctatttttat tctcgcgacg
     6961 tcgggagagt gtggcgtgtt gcggaaggcc ttgagtacgg catcgtcggc atcaacgaag
     7021 gcatcatctc gaacgagatg gcgccgttcg gcggcgtgaa agaatccggc cagggccgcg
     7081 aaggctcgaa gtacggcctc gacgactacc tcgagatcaa atacctctgc atgggcggcc
     7141 tcgacgactg acatccccca aaccaccagg tccgattctg gccattacac cgtcacccgc
     7201 ggcgtatact ttcgcggatg ctgcagaacg agatctatga acgcgcacgg cttgcacggg
     7261 acccacgttt cgacgggcag ttctacatcg ccgtaaaaac caccggcatc tattgccggc
     7321 cgatatgccc ggtgatgtcg ccgaagcgcg agaacatcac gtactttccc agcgccgcgg
     7381 cggccagcga ggccggctac cgtccctgcc tccgttgccg ccccgagtgt gcgcccggga
     7441 cccctgcgtg gggcggcact tcgaccacgg tgcgtcgcgg cctgcgactg atcgccggcg
     7501 gcgcgctcga tgaaggtaat gtcgaactgc ttgcagaccg gctcggtgtg accagccgcc
     7561 acctgcgccg cctgttcacc cggcacctgg gcgcatcgcc gctcgcggtc gcgcatacgc
     7621 agagactgca tttcgcgaag cgcctgatcg acgacacagc gttgccgatg acacacatat
     7681 cggcggccgc gggctaccgc agtgtgcgcc gcttcaacga cgcgttcatc aagacctacg
     7741 gacgttcgcc gcgtgagctg cggaaggttg gcgtcaacgg aagcggtgcc aggaaatccg
     7801 ccgcgcttgt cttgcgcctg ccgtaccgcg cgccattcga ctgggcggca atgc
//
LOCUS       DASUOM010000060         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1372943,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000060 DASUOM010000000
VERSION     DASUOM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372943"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..913
                     /locus_tag="VFY27_01320"
     CDS             143..913
                     /locus_tag="VFY27_01320"
                     /inference="COORDINATES: protein motif:HMM:NF005473.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5764178.1"
                     /translation="MGRLQNKVALVTGAAQGLGAATATLLAREGAKVLLTDINVEGVE
                     QRAAEINARDPGSAKAFEQDVTDENQWIKAIEAARRRFGGLHILVNNAGVGAVANIEE
                     ETLDNWRRVHTIDLDSVFLGSKHAIRLIAASGGGSIVNISSISGIIAGHNLAAYNSAK
                     AAVRHLSKSIALHCAKAGNGIRCNSVHPVFIDTPILDRIGAGQDRAVALEKLARQIPL
                     GRVGRPDDVAYAVLYLASDESAFVTGAEIRVDGGISAM"
     gene            924..2144
                     /locus_tag="VFY27_01325"
     CDS             924..2144
                     /locus_tag="VFY27_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5764179.1"
                     /translation="MTRKVIIAGAGHAAGQAVATLRQKKWPGEIVLAGEEPWLPYQRP
                     PLSKKFLAGELPAERLYVKPPAFYEDPSITLKLETRVDGIDRAARSLLFKGGGSLGYD
                     VLILALGARARRLPPLPGSDLAGIHYLRNVQDVQQIQQGLIEGRHLVIIGAGYIGLEV
                     AAVASRLGLKVTVVEMQDRVMKRVVAPPVSAFYAREHREHGVELRLQTGLAGFSGQEQ
                     VDGVNLADGSVLPADLVVIGIGIVPNTEVAAAAGLEVSDGIVVDEHCRTSDERIYAIG
                     DCTMHPNSLIGRSVRLESVQNALEQARTAASNICGEDSRYAEIPWFWSDQYDLKLQIA
                     GLSQGYDSAVVRGDPGKRSFSCVYLSGNRILAVDAINNPKDFLQSKKLIADRARMDLE
                     RLADASIELKDLAV"
     gene            complement(2265..>2696)
                     /locus_tag="VFY27_01330"
     CDS             complement(2265..>2696)
                     /locus_tag="VFY27_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764180.1"
                     /translation="VLVTLLGDGRGNASGLGVKFSSDSERTSLLKGEALLDSAWHVFL
                     PESGTLFIDQTENLWSYVRDIVVPARLSSADSWRGTWYGVTTAGPTALGTAKVAGGTG
                     RFADVATEAVEAINARAYSALQGPVSATASLTIVAVEPVAH"
BASE COUNT          520 a          840 c          864 g          472 t
ORIGIN      
        1 gccaaggacg gcgggatttc acgatctgga cccactgtcc cgccactctg ctgaaggaac
       61 ccggcctgct cgcgattcgc gctggttcgt gtttgaatcg cgcgcaggcg ggtgacccgc
      121 acttgattac cggaaatggc aaatggggcg actacaaaac aaggttgcat tggtcactgg
      181 cgccgcccag ggacttggcg ctgccactgc cacgcttctc gcccgcgagg gagcgaaggt
      241 gcttctgacc gacatcaacg tcgaaggtgt ggagcagcga gccgctgaga tcaatgcgcg
      301 tgatcccggc agcgcgaagg ctttcgaaca ggatgtcact gatgagaacc agtggatcaa
      361 agcgattgag gctgcgcgca ggcgcttcgg cggcctgcac atactcgtca acaatgcagg
      421 cgtcggcgct gttgcgaaca tcgaagaaga aacgctggac aactggcggc gcgtacatac
      481 gatcgatctc gacagcgtct ttctcgggag caagcacgcg atacggctga tcgcggcgag
      541 cggaggggga tccattgtga atatctcctc gatctcaggg attatcgccg ggcacaacct
      601 cgcggcgtac aactcggcaa aagctgcggt ccggcacctg agcaagtcca ttgcgctgca
      661 ctgcgcgaag gccggcaacg gaatccgctg caactcggta catcctgtat ttatcgatac
      721 gccgattctc gacaggatcg gtgcgggcca ggaccgcgcg gtggcgctcg aaaaactggc
      781 gcgccagatc ccgctgggcc gcgtgggccg gccggatgac gtggcctacg ccgtccttta
      841 cctcgcttcc gacgaatctg ctttcgtgac cggtgcggaa atccgcgtcg acggcggcat
      901 cagcgcgatg taggcggcgg agagtgacca ggaaagtgat catcgcgggt gcgggacacg
      961 cggccggaca agccgttgca acgcttcgcc agaaaaagtg gccgggcgag atcgtgctgg
     1021 caggcgagga gccgtggctc ccctaccagc gccctccgct gtcgaagaag ttcctggccg
     1081 gagaactgcc ggcggagcgc ctgtacgtca agccgccagc gttctacgag gacccgtcca
     1141 tcacgctgaa actcgagacc cgggtggatg gaatagaccg tgcagcgagg tcgctgctct
     1201 ttaagggcgg cggcagcctc ggctacgacg tgctcatcct tgcgctcgga gcccgcgccc
     1261 ggcgactgcc gccactgccg ggcagcgatc ttgccggaat tcattacctc cgcaacgtcc
     1321 aggacgtgca gcagatccag caaggcctga tcgagggccg ccatctcgtc atcataggcg
     1381 cgggatacat cggcctcgaa gttgctgccg tcgcctcccg gcttgggctc aaggtaaccg
     1441 tggtcgagat gcaggaccgg gtcatgaaac gcgttgtcgc cccgccggtg tccgcgttct
     1501 acgcgcgtga acatcgcgaa cacggcgtcg agctcaggct ccagaccggc cttgcgggtt
     1561 tcagcgggca ggagcaggtg gatggcgtaa acctggcgga tgggtcggtc ctgccggccg
     1621 acctggtggt gatcggcatc ggcatcgtcc cgaacaccga agtcgcagcc gcagcaggac
     1681 ttgaagtctc ggacggcatc gtcgtcgatg aacattgccg aacctccgat gaacgcatct
     1741 acgccatcgg cgactgcacc atgcatccca acagcctcat cggccgaagc gtcaggctcg
     1801 aatccgtgca gaacgcgctg gagcaggcgc ggactgccgc atccaatatc tgtggagaag
     1861 attcacggta cgcggagata ccctggttct ggtccgacca gtacgacttg aagctgcaaa
     1921 tcgcggggct ctcgcagggt tacgacagcg ctgttgtacg tggcgatccc gggaagcgct
     1981 ccttttcctg cgtgtatctc tccggcaacc gcatcctcgc cgtcgatgcg atcaacaatc
     2041 cgaaagattt cctccagtcg aagaaactga tagccgatcg ggcgcgaatg gacctggagc
     2101 ggctcgccga cgcgagcatc gaactgaaag acctggccgt ctgagaagcg gtgcgatcgg
     2161 accactccag ggactgatcc gggagatgcg aattcgcgga gctctgtcgc actcgcccgc
     2221 gtggcgctcc tacagcgctt cctcagcttt tgtgcaggcc ccggctagtg tgccacaggc
     2281 tctacggcaa ctatcgtgag gctggcggtg gccgacaccg ggccctgaag cgcggaatac
     2341 gcgcgggcgt tgattgcctc gaccgcttcc gttgcgacat ctgcaaaccg gcctgtaccg
     2401 cctgcaactt tcgcagtgcc gagcgcggtt ggccccgccg tggtcacgcc gtaccaggtg
     2461 ccccgccagc tgtcggcgga actcaagcgc gccgggacga cgatgtcgcg cacgtaggac
     2521 caaaggttct ctgtctggtc gatgaacaac gtgccgctct cgggcaggaa gacgtgccag
     2581 gcgctgtcca gcagggcttc gccttttagc aaactggtgc gttccgagtc ggaggaaaac
     2641 ttgacgccca ggccgctggc attcccgcgg ccgtcgccga gcaacgtaac cagtac
//
LOCUS       DASUOM010000061         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1614008,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000061 DASUOM010000000
VERSION     DASUOM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614008"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="VFY27_01335"
     CDS             <1..2061
                     /locus_tag="VFY27_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5764181.1"
                     /translation="FGPGLARNVGLDPGSIDAPDEKVRALETKLATTQREAAALSEQL
                     AAAQQNLERAEQDLEQQQVAEKRLQDELAAAQAKYEVVAASEGERDALKQGVDRSSTE
                     LREQQMVVARLQDEINRNRRQIAAYQSDFERIAQLEAQLKAQTERYETTSRELEQARA
                     ALADSNERLAAQQRIFEAERRELEATRSNLDRTAELSTETQASLQAELKDREARLAGV
                     AGKLDEMRQEVDSFRAQSVSLQDELQALRARNAELAASREEAELYQREAERLKALLAE
                     TQDQLAAIESAPDDRAEKVDQMQAELTRVRQEVARYRERITELEGNQVIVADLAGPTI
                     QLIDPAAFSTRGTSEITVSSVDQYQIVGKVTAPAGVLSVLVNRHEADLNDSNVFRSVV
                     DLSGEKTPIHIAAIDKQGKRTEQVLSLISEAARQAAAKPKLPQVEFGEFYALLIGNEN
                     YEMLPDLVTPKEDVRQIGAVLSERYGFHTTVLEDSGRQQIMDSMYELLGRLTSEDNLL
                     IYYAGHGEYVTDTDRGVWLPVDASPSSPANWISNVEINDYLKQIRAKQIVVIADSCYS
                     GALTRSALINTRPGLTDEEYEAHLEKMARIPARVVLTSGALAPVLDSTSPDSQHSIFA
                     AALIEILTQNDTVLSAQDLGRTIAAKVSLAATKVGYEQEPQYAPLNHSNHQGGDFFFV
                     PEYF"
     gene            2185..2607
                     /locus_tag="VFY27_01340"
     CDS             2185..2607
                     /locus_tag="VFY27_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764182.1"
                     /translation="MTDKPIIGIALGGGAARGWAHIGVLGALREPADAEHQEELSYLD
                     RMQHYMRGLLEPSSDPPDRGPALLDVVNSAINIMQERVARSRLAGDPPELEIVPDVQD
                     IRLMDFHRAALAVERGVEAVHARAADISRLLQISEANG"
     gene            2623..3741
                     /locus_tag="VFY27_01345"
     CDS             2623..3741
                     /locus_tag="VFY27_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764183.1"
                     /translation="MQGIFSSRVVRVYVVPGAVFQSVMIGGGYGTGREIVEFFTSFGL
                     LGGLLGLGVAFLVLSLVLALTFELARSFRVYDYRNFFKLLLGRGWVAFEILVVLLFLL
                     ILAVLASAAGNILRDNFGIPYVAGLSVMLLVIGVLTFYGRELIARVLTFWSLVLYIVF
                     GTFFVAVLGSGWEPIADAFAGGEVVAGWWRSGFTYAMYNLAVAPLLLYVTRGFETRAQ
                     ALTSGVVAGAIAMLPAVMFHISFFAAYPGILHQALPAYWLISELGLTWLLVIFSIVLF
                     GTFIETGAGMLQGINERIDTWLAERRGKGLGRTAHAILAVSAILVSALLSLWGITSLI
                     AQGYGTIAWGFFAVFMVPLVTVGVYRIAMARDKSFEKA"
     gene            3752..>5403
                     /locus_tag="VFY27_01350"
     CDS             3752..>5403
                     /locus_tag="VFY27_01350"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5764184.1"
                     /translation="MNWMGRSRRNLCGLLAAGVFAAASLSGGCGEQEPAAATSTAERT
                     VYDVVIRGGTVYDGLGGDPVSADAAIDGDRLAVIGDLSGARGRTEVDAGGLAVAPGFI
                     NMLSWAVDSLIEDGRAQSDVRQGVTLEVFGEGESWGPWNESLKQVMKEGQADIRYDIE
                     WTTLNEYLEFLENKGVSVNVASFIGATTVRENVIGFEDRVPTQEELEEMQLLVRQAME
                     EGALGVGSSLIYAPAFYAKTPELIALAKAAAEYDGMYISHMRSEGNQLLQAVDELLQI
                     AREAKIRAEIYHLKAAGRDNWGKLDQVFQKVEAARAAGLEITADIYPYTAGATGLDAA
                     MPPWVQEGGTQQWIERLKDPEIRERVVAEMKTPSDDWENLYLAAGADKLIVIGFKNPD
                     LKHLTGKTLGEVARERGTSPEETAIDLVIEDGSRVGTVYFLMSEDNIRKKIQRDWVSV
                     GSDADARAPEGVFLESNPHPRAYGSFARFLGKYVRDEQLISLAEAVRRLTSLPAGNLR
                     ITGRGSLKAGNFADVVVFDPKAIQDHATYENPHVYSTGVLHV"
BASE COUNT         1075 a         1588 c         1751 g          989 t
ORIGIN      
        1 ttcggccctg gtctcgcacg caacgtcggc ctcgaccctg gatcgatcga tgctcccgat
       61 gaaaaggtcc gggctctgga aacgaaactt gcgaccacgc agcgcgaggc agcggctttg
      121 tcggagcagc ttgcggcggc acagcagaac ctcgagcggg cggagcagga ccttgagcag
      181 cagcaagtgg cggaaaagcg cctgcaggac gaactggctg ccgcacaagc gaagtatgaa
      241 gtcgtagcgg ccagtgaggg agaacgggat gccctgaagc agggtgtcga ccgaagcagc
      301 accgaactgc gagagcaaca gatggtcgta gcgcgcctgc aggatgagat caaccggaat
      361 cgccggcaaa tcgctgccta tcaatccgac ttcgagcgca tcgcccagct ggaagcccaa
      421 ctgaaggcgc agacggagcg ctacgagaca acgagcaggg aactcgagca ggcgcgggcg
      481 gcattggccg actcgaacga gcgactggcg gcgcagcagc gcattttcga agcggagcga
      541 agggaactcg aggcgacacg cagcaacctt gatcgtacgg ccgaattgtc gacggagaca
      601 caggcatcgc tgcaggctga actgaaggat cgggaagcga ggctcgccgg cgtcgccggc
      661 aagctcgacg aaatgcggca ggaagtcgac tccttcaggg cccagtccgt ttccctgcag
      721 gacgagttgc aggcgctgag agcgcggaat gcggaactgg ccgcttcgcg cgaagaagcc
      781 gagctttacc agcgcgaagc cgagcgcctg aaagcgcttc tcgcggaaac ccaggaccag
      841 cttgctgcaa ttgaatccgc gcctgacgac agagctgaga aagtcgacca gatgcaggcc
      901 gaacttacgc gcgtgcggca ggaggtagcc cggtaccgtg agagaatcac cgaactcgaa
      961 ggcaaccagg tcatcgttgc cgacctcgct ggtccgacga tccagctcat cgatccggct
     1021 gcgttcagta cgcgcggcac ttccgaaatc accgtgagtt ctgtagatca ataccagatc
     1081 gtcggcaagg ttaccgcgcc ggccggtgtg ttgagcgtgc tcgtcaaccg gcacgaggcc
     1141 gacctgaatg acagcaatgt gttccgcagc gtcgtggatc tgtccggcga gaaaacgcct
     1201 attcatatcg cggcaatcga caagcagggc aagcggaccg agcaggtctt gagtctcatc
     1261 agcgaagcgg cgcggcaggc tgcggccaag ccaaagcttc cccaggtaga gttcggcgag
     1321 ttttacgcgc tgctcatcgg aaacgaaaac tacgagatgt tgcctgacct ggttacgccg
     1381 aaagaggacg ttcggcaaat cggagccgtg ctgagcgaac gctacggctt tcacacgacc
     1441 gtgctcgaag acagcggtcg ccagcaaatc atggacagca tgtatgagct gctgggccgc
     1501 cttacgtccg aggacaacct cctgatctac tatgccgggc acggtgagta cgtgacggat
     1561 accgatcgcg gggtatggtt gccggtcgat gcgagccctt cgagtccggc gaactggatc
     1621 tcgaacgtcg agatcaacga ttacctgaaa cagattcggg cgaagcagat cgtcgtcatc
     1681 gccgattcct gttattcggg tgcattgacg cgttccgcgt tgatcaacac ccggcccggc
     1741 ctgactgacg aggagtacga agcgcatctc gagaagatgg cacggattcc tgcaagggtc
     1801 gtattgacct cgggcgcgct ggcgccggtt ctcgattcga cgagtccaga ctcacagcat
     1861 tcgattttcg cagccgccct cattgaaatc ctgacgcaga acgacacggt gctttcggcg
     1921 caggacctcg gtcgcacgat tgctgcgaag gtcagtcttg ccgcaaccaa agtcgggtac
     1981 gaacaggagc cgcagtacgc tccgttgaac cattcgaatc accagggcgg cgatttcttt
     2041 tttgtgccgg agtatttctg atccccgtca tcgtcaccgc gctgtaagtc cgccgccgac
     2101 gctaccccgg gtcgctcgca ggacgggcaa gctataccat cctcgatcat cgaacaaacc
     2161 cggtgccgtg gtcacctatc cataatgact gacaaaccca ttatcgggat cgcgctgggc
     2221 ggcggtgctg ccaggggatg ggcgcacatc ggtgtcctcg gggccttgcg cgagcctgcc
     2281 gatgcggagc accaggagga actaagctac ctcgaccgca tgcagcacta catgcgcggc
     2341 ctgctggagc cgagcagcga tccaccggac cgcggtcctg ccctgctgga cgtggtgaac
     2401 agcgccatca acatcatgca ggagcgagtc gcccgcagcc gccttgcagg agacccgccc
     2461 gaactcgaga tcgtgcccga cgtgcaggac atccggctga tggattttca tcgtgccgcg
     2521 ctcgccgtcg agcgcggagt ggaagccgtg cacgcccgcg cggccgacat ctcccgccta
     2581 ctgcagataa gcgaagccaa cgggtaagga aaaaggggag tagtgcaagg aatattctct
     2641 tcgcgtgtcg tgcgcgtgta cgtcgtgccg ggtgcggtat tccagtcggt gatgattggc
     2701 gggggctacg gcaccgggcg cgagattgtc gagttcttca cgtcattcgg gttgctcggc
     2761 gggctgctgg gcctgggcgt cgcgttcctc gtgctgtcac tggttctcgc gcttacgttc
     2821 gaactggctc gctccttccg cgtttacgat taccgtaact ttttcaagct gctgctgggc
     2881 cgcggctggg ttgcattcga aatcctggtc gttctcctgt tcctgttgat actcgcggtg
     2941 ctggcgtcgg cggccggcaa tatcttgcgc gacaacttcg gcatacccta cgttgccggt
     3001 ttatcggtga tgttgctcgt gatcggcgtg ctcacgttct acggaaggga gctcatcgcc
     3061 agagtcctga cgttctggtc cctggtactc tacatcgtgt tcggcacttt cttcgtcgcg
     3121 gtgctcggca gcggctggga gccgattgcc gacgcgttcg caggcggcga agtcgttgcg
     3181 ggctggtggc gcagcgggtt cacgtacgcg atgtacaacc ttgcggttgc acctttgctg
     3241 ctttatgtca cccgcggttt cgaaacccgc gcccaggcgc tgacgtcggg ggtcgtggcc
     3301 ggtgcgatag ctatgcttcc ggcagtgatg tttcacatct cattttttgc cgcatacccc
     3361 gggatactgc accaggcgct gccggcatac tggctcatct ccgagctggg actgacctgg
     3421 ctgctcgtca tcttctccat cgtcctgttc ggcaccttca tagagaccgg tgccgggatg
     3481 ttgcaaggta tcaacgagcg aatcgatacc tggcttgcgg aacgacgagg caaggggctc
     3541 ggccggacgg ctcacgccat actcgccgta tccgccattc tcgtgagtgc cctcctgtct
     3601 ctctggggaa tcacgtcgct gattgcgcag ggctacggca cgatcgcgtg gggattcttc
     3661 gccgtgttca tggtgccgct ggtgacggtt ggggtttacc gaatcgcgat ggcaagggac
     3721 aaatcgtttg agaaagcttg aggagagcaa tatgaattgg atgggtcgat cgcgacgtaa
     3781 cttgtgcgga cttcttgctg cgggcgtttt cgctgccgcg agcctctccg ggggatgcgg
     3841 cgagcaggag ccggccgcgg cgacgagtac ggcggaacgc accgtttatg acgtcgtcat
     3901 ccgcggcggc accgtgtatg acgggctggg tggagaccct gtgtccgcgg acgcggcgat
     3961 agacggggac aggcttgcag tcatcggtga cctttccggc gcccgcgggc gtacggaagt
     4021 cgatgccggc ggactcgccg tggcgccggg attcatcaac atgctcagct gggccgtcga
     4081 ctcgctgatc gaggacggca gggcgcagag cgacgttcgg cagggcgtga cgctcgaggt
     4141 cttcggcgag ggcgaatcgt ggggcccctg gaacgaatcg ctcaagcagg tcatgaagga
     4201 aggccaggcc gatatccggt atgacatcga atggacgacg cttaacgagt acctggagtt
     4261 tctcgagaac aagggcgtct ctgtgaatgt cgcgtccttc atcggcgcca ccacggtccg
     4321 tgaaaacgtg atcggcttcg aagaccgcgt tcccacgcaa gaggaactcg aagagatgca
     4381 gctgctcgtg cggcaggcga tggaggaggg cgcgctcggt gtcggctcgt cgctgattta
     4441 tgcgccggca ttttatgcga aaacgcctga actcatcgcg ctcgccaaag ccgctgcgga
     4501 atacgatgga atgtacatat cgcacatgcg aagcgaaggg aaccaattgc tgcaggctgt
     4561 cgacgagctc ctgcaaatcg cgcgggaggc caagatccgg gcggagatat atcacctcaa
     4621 ggccgcgggc cgcgacaact ggggcaagct cgaccaggtg ttccagaaag tcgaggcggc
     4681 acgcgccgcc ggactcgaga tcaccgcgga catctacccg tacaccgctg gcgccaccgg
     4741 gctcgatgcc gcgatgccgc catgggtgca ggagggtgga acgcagcaat ggatcgaacg
     4801 gctgaaggac ccggaaattc gcgagcgggt tgtcgcggaa atgaagacac caagcgacga
     4861 ctgggagaac ctgtacctcg cggccggcgc cgacaagctg atcgtcatcg gcttcaagaa
     4921 cccggacctg aagcacctga cgggcaagac gcttggcgag gtcgcccggg agcgcggaac
     4981 ctcgcccgaa gaaaccgcga tcgatctcgt gatagaggac ggcagccgcg tcggtaccgt
     5041 gtacttcctc atgtccgagg acaacatccg caagaagatc caaagagact gggtgagcgt
     5101 cggctcggac gcggacgcga gggcgccgga gggcgtgttc ctggaatcca acccgcaccc
     5161 gcgcgcctac ggcagcttcg cgcgtttcct cggcaagtat gtgcgcgacg agcaactgat
     5221 ctccctggcg gaagctgtca ggcgcctgac gtcgctgccc gccgggaacc tgagaatcac
     5281 gggacgcggc tcgctcaagg cgggaaattt cgccgacgtg gtggtgttcg accccaaggc
     5341 gatccaggat catgcgacct atgagaatcc gcacgtttac tcgaccggcg tcctgcacgt
     5401 ctt
//
LOCUS       DASUOM010000062         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_766426,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000062 DASUOM010000000
VERSION     DASUOM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766426"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFY27_01355"
     CDS             complement(<1..352)
                     /locus_tag="VFY27_01355"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5764185.1"
                     /translation="MLRFPAAVERDPSVEAWFGSRTGGLGAIARPWYERMRQCGNDVR
                     ELLHDGHPTACVGDAAFGYVNSFTAHVNVGFFLGAQLADPARLLEGTGKNMRHVKLRP
                     GTELDREALATLIEA"
     gene            complement(373..780)
                     /locus_tag="VFY27_01360"
     CDS             complement(373..780)
                     /locus_tag="VFY27_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5764186.1"
                     /translation="MKSAAEQPTVSVATLKAIADAFNAHDLDAIMEFFADDCSLDMPR
                     GPDPWGQRFTGKAAVREGLAMRFKGLPDVHYSDDRHWVSGNMGVSEWLLTGTMPGEVR
                     IRVRGCDHWEFRDGKVIRKDSYWKIVEKSAQRN"
     gene            complement(797..1333)
                     /locus_tag="VFY27_01365"
     CDS             complement(797..1333)
                     /locus_tag="VFY27_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEX5764187.1"
                     /translation="MHRGKLLASLKSRFDKNMQRHAGVAWDDVLARLEGSPAALKSLG
                     EMEASGGEPDMIGQDKAAGQYVFCDCSAESPLGRRSLCYDREALSSRKEHKPVGSAVE
                     MAAAMGIELLTEEQYRELQQLGEFDTKTSSWIATPRDVRLLGGALFCDRRYGRIFAYH
                     NGAQSYYAARGFRGLLRA"
     gene            complement(1326..1625)
                     /locus_tag="VFY27_01370"
     CDS             complement(1326..1625)
                     /locus_tag="VFY27_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764188.1"
                     /translation="MAPDPTTPAGVASRSDTMLSRVLGGMSVFTMLMTVPQVLTIWVG
                     HQAAGVSVVSWSAYLFSAILWFWYGLQKRDKNIYLPCVGWVGLDTAVIVGALVYA"
     gene            complement(1652..2137)
                     /locus_tag="VFY27_01375"
     CDS             complement(1652..2137)
                     /locus_tag="VFY27_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5764189.1"
                     /translation="MAKSPKASIFLWYNGDAEEAARFYAKTFPDSSVGAVHRAPGDFP
                     SGKQADVLMVEFTVMGIPCLGLNGGPAFKHNEAFSFQVATADQAETDRYWNAIVGNGG
                     QESACGWCKDRWGLSWQITPIALTKAVTDPDRAAAKRAFEAMMQMKKIDIAAIEAARR
                     G"
BASE COUNT          382 a          729 c          651 g          418 t
ORIGIN      
        1 cggcctcgat cagggtggcg agcgcctcgc ggtcgagttc ggtgcccggc cgcagcttca
       61 cgtgacgcat gttctttccc gtcccttcga gcagccgcgc gggatccgca agctgcgcgc
      121 cgaggaagaa gcccacgttg acgtgcgccg tgaacgagtt gacgtagccg aatgcggcgt
      181 cgccgacgca tgcggtggga tggccgtcgt gcagcaactc gcgcacatcg ttgccgcact
      241 ggcgcatgcg ctcgtaccac gggcgcgcga tcgcaccgag gccgcctgtc cggctgccga
      301 accatgcctc gaccgaggga tctcgctcga cggccgccgg aaatcgcagc aatcggactc
      361 gactacgcac ggctagttgc gctgcgcgga cttctcgacg atcttccagt acgaatcttt
      421 cctgattacc ttcccgtccc ggaactccca gtgatcgcat ccgcgcactc gaatccgcac
      481 ttcgccgggc atggtgccgg tgagcaacca ctcggacacg cccatgttgc cgcttaccca
      541 gtgccgatcg tcactgtaat ggacgtcggg aaggcccttg aagcgcatcg ccaggccttc
      601 tcgcacggcc gctttgcccg tgaagcgctg gccccaggga tccggccccc gcggcatgtc
      661 cagcgagcaa tcgtcggcga agaactccat gatggcgtcc aggtcgtggg cgttgaacgc
      721 atcggcgatc gccttcagag ttgcgaccga aaccgttggc tgttcagccg cgctcttcat
      781 ttcagctttc tcctattcag gcgcgtagca gcccgcgaaa gccgcgtgcc gcgtagtacg
      841 actgcgcgcc gttgtgatac gcgaatatcc tcccatagcg ccgatcgcag aagagggccc
      901 cgccgagtaa gcggacatcc cggggcgtcg cgatccagct ggaggttttc gtatcgaatt
      961 ccccgagctg ctgcagctcg cggtattgtt cctccgtaag gagctcgatg cccatcgcgg
     1021 ccgccatctc gactgcgctg cccaccggct tgtgctcctt tctcgagctc aatgcctcgc
     1081 ggtcgtagca caggctccgg cgcccaagcg ggctctccgc agagcagtcg cagaaaacgt
     1141 attggccggc ggccttgtcc tggccaatca tgtccggttc accgccggag gcctccattt
     1201 cacccagcga tttcagggca gccggacttc cttccagcct ggccagaacg tcgtcccacg
     1261 cgacccctgc gtgccgctgc atgttcttat cgaatcggct cttcagcgac gccagcagct
     1321 tgcccctatg catacacgag cgcacccacg ataaccgctg tgtccaggcc aacccatcca
     1381 acgcacggca gataaatgtt cttgtcgcgt ttctgcaggc cgtaccagaa ccaaaggatg
     1441 gcggaaaaga ggtacgcact ccacgacacc acggaaacgc cggcggcctg gtggccaacc
     1501 cagatggtca gaacctgcgg aaccgtcatg agcatggtga agaccgacat gcctcccagc
     1561 acgcgactaa gcatcgtgtc ggagcgggat gcgacaccgg cgggagtggt ggggtctggt
     1621 gccatggacc tcgtcgatga tattcgccgc ctcagccgcg gcgcgccgcc tcgattgcgg
     1681 cgatgtcgat ttttttcatc tgcatcattg cctcgaaggc gcgcttggcg gccgctctgt
     1741 cggggtcggt caccgctttc gtcagggcga tcggcgtaat ctgccacgac aggccccatc
     1801 ggtccttgca ccagccgcac gcactctcct ggccgccgtt gccgacgatc gcgttccagt
     1861 aacgatccgt ctcggcctgg tcggcggttg ccacctggaa cgagaatgct tcgttgtgct
     1921 tgaacgcagg tccgccattg agcccgaggc agggaatccc catcacggta aattcgacca
     1981 tcaatacatc cgcttgcttg ccggacggaa agtctcccgg tgcgcggtgc accgcgccga
     2041 cggacgaatc gggaaacgtc ttcgcataaa accgcgccgc ctcctcggca tcgccgttgt
     2101 accagaggaa gatggaggct tttggtgatt tggccattgc atttattcct gattgggaga
     2161 ggccgatgtc agcggctggg
//
LOCUS       DASUOM010000063         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1717769,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000063 DASUOM010000000
VERSION     DASUOM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717769"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..2392
                     /locus_tag="VFY27_01380"
     CDS             323..2392
                     /locus_tag="VFY27_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764190.1"
                     /translation="MTTHPLICLVGITLGFGLGHPSSLFASEHTPSAPLRFSVSEGRV
                     HNEFFRDGQVAAHLVLTSGPAPRLVVAFPAGNSGTAVWFDSASGSFAWLPEAGITAAH
                     RNVKEGTLRGVSAELEATGAPITIRHAIMSSVRVIRDYGYTGETPAEVMVPPQLTENS
                     IVWQRQRLDGEPGYYLSLELLGGRISGDSETVRLLPDGDGRFRFRVTALTGEEPLMPI
                     VEDELLTSAAQPDARLRQILGFLSYEGKLLAGSWRFNTYFGRDTLMSLELLMPVLQPA
                     PVEAGLGAVLERLNAAGEVAHEEDIGEYAVLRHIREDDEASDTPIYDYKMIDDDFLLP
                     IVSARYLLATSTGRERAADFLARTTSSGERYGAALVRNFQFILSASSAFARNPRWSNL
                     VALKPGVYVGNWRDSEDGLGGGRFPYDVNGIFVPAALAAIGRLHDSGILEAYIPQDAG
                     AAISHAGREAAVWQRYAPPYFEATFSSEIARAEATRYARRIGVPSTAALTALGDDPVT
                     FHAVALDEAGAPVPILNSDEAFALLFLDVAPAKVERIIESLMRPFPAGLMTDVGPVVA
                     NPAYANDELEPLFGRSRYHGTVIWSWQQAMLAAGIARQLERGDLTTSARNALISARAR
                     MESVMAGSRTRRGSELWSWSQQNGRYVVEPFGQRTEDETESNAAQLWSTVYLAAASQL
                     TRRAAGE"
     gene            complement(2367..>3577)
                     /locus_tag="VFY27_01385"
     CDS             complement(2367..>3577)
                     /locus_tag="VFY27_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5764191.1"
                     /translation="FDGGAFELAQTAGWFSVDGHGVDYRVLPIVDVLKEAGRPSSLYE
                     KFASSNPEASYFQDLEWLRPGDRLNVPALFFDSWYDYGAAETLALFGMLRQQSVTATA
                     RENQFVIIAPGTHCGYREATERTVVGERDLGDARIDVLDLQIRWFDYWLKGIDNRVTD
                     RPRIQYYLMGANRWRYADTWPIAGTRFTKLYLGSRGDANGLDGDGTLSHEPPSRSASD
                     TFEYDPASPVPSHGGHACCTGTDTEAGAYDQTQIEMREDVLVYTSAKLGEGIEVTGPL
                     RLTLYVSSSAPDTDFTAKLVDVYPDGRAFNIQEGALRMRYRGGFDKNLRMQAGEVYEA
                     QIDLHVTSNYFGRGHRIRLDISSSNFPRWDRNLNTGGNNYDEKEFIVARNTVHHTPEH
                     PSFILLPLVE"
BASE COUNT          635 a         1087 c         1124 g          731 t
ORIGIN      
        1 ccagaagccg atggcgaaca atatggccag gtaagccgtg aggattccga tgatcagggg
       61 gtcgttcatc ggtcaagctt catcgcgcga agcgctccgt gacctgcctg cgttcttcag
      121 gcagtacagg attgtcatga gtaaccacgg aagcgtggag caggcgatga gaatccaggc
      181 cgtggccggc agttcatgca tgatcgggtt ccccgcctct tcttcgttgg cctgaactct
      241 gctgcggcgc accgggggtg tcaaggcgct cgctgcgcca gccttcgatg aggtgtcgcg
      301 gatgctatat tgtgtcgtgc ctgtgacaac gcatccgctg atctgcctcg tcggaattac
      361 ccttggattc ggcctcgggc atccttccag cctgttcgca tccgaacata cgccatccgc
      421 tccactgcgt ttctccgttt ccgaaggacg cgtgcacaac gagtttttcc gggacggtca
      481 ggtcgccgca cacctcgttc tcacatcggg cccggcgccg cggctggtgg tggcgttccc
      541 tgccggtaac agcgggacgg ccgtgtggtt cgactcggca tccggttctt tcgcctggct
      601 acccgaagcc gggataaccg ccgcacaccg caacgtcaag gaaggcactc ttcgaggcgt
      661 gtccgcggaa ctcgaagcga ccggtgcgcc gatcacgatc cgccacgcga tcatgagcag
      721 cgttcgcgtc atccgcgatt acggctacac aggtgagact ccggcggaag tcatggtccc
      781 cccgcagcta acggaaaact cgatcgtatg gcagcgccaa cggctcgatg gcgagccggg
      841 ttactacctg tccctcgaac tgctgggcgg caggatttca ggcgacagcg aaaccgtcag
      901 gttgctgccg gatggcgacg gacgctttcg ctttcgggtg acggcgctaa ctggcgagga
      961 gccgctcatg ccgatcgtcg aggacgagtt gctgacttcg gcagcgcagc ccgatgcgcg
     1021 cttgcggcag atcctggggt tcctgagtta cgaggggaaa ctgcttgccg ggtcctggcg
     1081 attcaacact tacttcggcc gcgacacgct gatgtccctc gagctgctga tgccggtact
     1141 gcaacccgcg ccggtggaag ccggcttggg agccgtactc gaacgcctga acgccgcagg
     1201 cgaagtcgcg cacgaggaag acatcggtga atatgctgtc ttgcgtcaca tccgcgagga
     1261 cgacgaagcg agcgatacgc cgatctacga ctacaagatg atcgacgacg attttctgct
     1321 acccattgtc tcggcccgtt acctgctggc gacttcaaca ggacgtgaac gcgcggcgga
     1381 tttcctggcg cgtactacgt cgtcgggtga gcggtatgga gccgcgctcg tacggaactt
     1441 tcagtttatt ttgtcagcct cttcggcttt tgcccgaaac ccgcgatggt cgaacctggt
     1501 cgctcttaag ccgggagtat acgtcggcaa ctggcgcgac agcgaggacg gcctcggggg
     1561 cggacgcttc ccttatgacg tgaatggcat ctttgtgccg gccgcactgg cggccattgg
     1621 caggttacac gacagtggca tcctcgaggc ttacatcccg caagatgctg gtgccgcgat
     1681 ttcgcacgcc ggtcgcgaag ccgctgtatg gcagcgatac gcgccacctt acttcgaagc
     1741 gacattttcg tcggagatcg ctcgcgccga agcaaccagg tatgcccggc gtatcggggt
     1801 gccgtcgacc gcggcgctga ctgcgcttgg agatgatccg gtcacctttc atgccgtcgc
     1861 gctcgatgag gcgggcgcgc cggtgccgat cctgaattcg gacgaggcat tcgcgttgct
     1921 gtttcttgac gttgcaccgg cgaaagttga gcgcatcatc gaatcgctca tgcggccatt
     1981 cccggccggg ttgatgaccg acgtcggacc ggtagttgcg aacccggcgt atgccaatga
     2041 tgaactggag ccgttatttg gccgctctcg ttatcacgga accgtgatct ggtcctggca
     2101 gcaggcgatg ctcgctgcgg gcattgctcg gcagctcgaa cgcggcgacc tcacaacctc
     2161 cgcgcgaaac gcgctcatca gtgcccgtgc gcgtatggaa tcagtcatgg ccgggtcgcg
     2221 cacccggcgc ggctccgagc tgtggtcctg gtcgcagcaa aacggccgct acgtcgtcga
     2281 gcccttcggg cagcggacgg aagacgaaac ggagtcgaac gctgcgcagc tctggagcac
     2341 ggtatacctg gcggcggcgt cgcagcttac tcgacgagcg gcaggagaat gaacgaaggg
     2401 tgctccggtg tgtggtgtac cgtattccga gctacgatga attccttctc gtcgtaatta
     2461 ttgccgcccg tattgaggtt gcgatcccag cgcgggaagt tcgagctgga gatgtccagg
     2521 cggatacgat gcccgcgacc gaaatagttg ctggtcacgt gcaggtcgat ttgcgcctcg
     2581 tacacttcgc ccgcctgcat gcgcagattt ttgtcgaagc caccgcgata tcgcatgcgc
     2641 aacgcgcctt cctggatatt gaaggcgcga ccgtccgggt agacatcgac gagcttcgcc
     2701 gtgaagtccg tgtcgggagc tgaagacgag acgtacaagg tcagcctcag cggtcctgtg
     2761 acttcgatgc cttccccgag cttagcgctt gtgtacacga gcacgtcttc gcgcatttcg
     2821 atctgtgtct ggtcgtaggc tccggcttcc gtgtcggtgc cggtacagca ggcatggccg
     2881 ccgtgcgaag gcaccggtga agccgggtcg tattcgaacg tgtctgacgc gctgcgcgaa
     2941 ggaggttcat gggacagcgt tccatccccg tccaggccgt tcgcgtctcc cctgctgccg
     3001 aggtagagct tcgtgaagcg tgtgccggct atcggccagg tgtccgcgta gcgccagcga
     3061 tttgcgccca tcaggtagta ctggatcctt ggcctgtcgg tgacgcggtt atcgatgccc
     3121 ttgagccagt agtcgaacca ccgtatctgc aggtccagga cgtcaatgcg cgcatcaccc
     3181 aggtcacgct cgccgacgac tgtccgttct gtcgcttctc gatagccgca atgagttccc
     3241 ggcgcgatga ttacgaactg gttttccctc gccgtcgcgg tgacggattg ctgtcgcagc
     3301 ataccgaaga gagccagcgt ctcggcggca ccgtagtcgt accatgagtc gaagaacagt
     3361 gcggggacgt ttagcctgtc gcctggtcgg agccattcga ggtcctggaa gtacgatgcc
     3421 tccgggttgc tggaagcaaa tttctcgtaa agggacgacg gccgccctgc ctctttcagg
     3481 acgtcgacga tcggcagcac gcggtaatcc accccgtgcc catcgacgct gaaccacccc
     3541 gctgtctgcg ccagttcaaa ggcaccacca tcgaacg
//
LOCUS       DASUOM010000064         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1127669,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000064 DASUOM010000000
VERSION     DASUOM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127669"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFY27_01390"
     CDS             complement(<1..1569)
                     /locus_tag="VFY27_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5764192.1"
                     /translation="MQQDDRASAPGEPASKGAGAPGRLNLFIPNIGQIAKGAYGREKF
                     RNDIFGGLTAAVVALPLALAFGVASGAGPIAGLYGAIAVGFFAALFGGTPAQVSGPTG
                     PMTVVMGAVVATHSHSLAEAFTIVILGGLLQILFGLLRVGKYVEYTPYSVVSGFMTGI
                     GVIIMILQTLPFLGLPASDHGVLGTLRELATLDPGAVDYRTFIFAAVALAIVVYWPKK
                     LHNRFPAPLGVLLIGTIVATFFLHGMPQIGEVPSGLPSFIVPQMPVAEMPLFIQAAFI
                     LALLGSIDTLLTSLVADSVTRTHHDSDKELIGQGIGNCMSGLIGGLPGAGATMRTIVN
                     VRAGGRTPVSGMLHAAILLALALGLGPVVAHVPHAVLAAILMKVGWDIIDWGYLKRMR
                     RAPREKFIIMLVTLTLTVFVDLIIAVAVGIILASFVTSRGLAVEQLKGLRQSVDADEL
                     DQLTGEERVMLRSANGKVLVTILHGSFSYASARELSRRASPKVTGYQASIYDFSSAGY
                     IDTSAALAIEEMIDL"
     gene            complement(1795..3204)
                     /locus_tag="VFY27_01395"
     CDS             complement(1795..3204)
                     /locus_tag="VFY27_01395"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5764193.1"
                     /translation="MKNGFLASVHNMVDRAFGTMDLPPGLAEQIKQTNSLYRVKFPVE
                     IRGEVQVFTGWRAVHSEHRLPTKGGIRYSKMVNQDEIEALAALMSYKCAIVDVPFGGS
                     KGGLEIDPADYQEHELKVITQRFAYELAKKGYISPSENVPAPDLGTSEREMAWIADTY
                     RMDFPDDINAIACVTGKPVTQGGIAGRTEATGRGIQYALQEFFRHPEDVKEAGLRGTL
                     AGKRVVVQGLGNVGYHAAKFLQEENDVRIVAIAERDGALVSEKGLDVENVHAFLRQSG
                     GVANYGDAEFIADSARALELDCDILIPAALENQINENNADRISAPLIAEAANGPTTYR
                     ADQMLHKKGTVILPDAFMNAGGVTVSYFEWVKNVSHIRFGRLDRRLEEARGRHIVRLV
                     EEMTGKTVPEHLSAPLLAGASEIDVVRSGLDDTMRLAYNQIRGIRRSRQNVPDYRTAA
                     YVSALQKIARSYLELGIGH"
     gene            complement(3390..4484)
                     /locus_tag="VFY27_01400"
     CDS             complement(3390..4484)
                     /locus_tag="VFY27_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5764194.1"
                     /translation="MERNQRRHGHPGREHRRESSRRPYSDEQETYRNPRNEYDDDRGR
                     DHRQSQYGRQDERNRDWEFSQDESNPYGASERGYGYGDQGFSDDSEYYEPGYAARRQG
                     QSRDTWDEPETGNFSDTRSRWPRDDRYRSEPRSQNWPHSRTGGRVSEYGYFGDQSEGY
                     GGRHQGGSRYGFGSRPQRGYQSGYGAQQQGSSQFGYLGGSGEPGESRYSSQYDYRREG
                     GSGGYGQQEGGTGHGNHRFDEDYSGRGPRNYSRSDERVKEDICDELSSDPRCNAEDIE
                     IEVKDGMVTLSGTVPSRKMKHRAEDIADAARGVKDVDNRIRVTGRVGQRGSSGGSEES
                     RSAGNEDMGSSNGRSRKSESQQSSRSSSRK"
BASE COUNT          870 a         1503 c         1435 g          958 t
ORIGIN      
        1 aagatcgatc atctcctcga tagccagtgc cgcgctcgtg tcgatatagc cggcgctgga
       61 gaagtcgtaa atggaggcct ggtagcctgt gaccttcggc gatgcccggc gcgaaagctc
      121 acgagccgac gcatacgaga atgagccatg gaggatcgta accagtacct ttccgttcgc
      181 ggagcgcagc attacccgct cttccccggt cagctggtcc agctcatcgg cgtcgacgga
      241 ctgccgcaaa cctttcagct gctccaccgc gagtccgcgc gaggtgacga agctcgcaag
      301 gatgatgccg acagccaccg caattatgag gtcgacaaac accgtcagcg tcagcgtcac
      361 cagcatgatg atgaacttct cgcgcggggc acgccgcatg cgcttcaggt aaccccagtc
      421 gatgatgtcc cagccgacct tcatcagtat cgccgcgagg acggcgtgcg gcacatgagc
      481 gacaaccggt ccgagtccaa gcgcaagtgc cagcagtatg gccgcgtgca gcatgccgga
      541 aacaggtgta cgtccgccgg cccgaacgtt gacgatcgtg cgcatggttg cgccggcgcc
      601 cggaagcccg ccgatgaggc cggacatgca gttaccgata ccttgcccga tgagttcctt
      661 gtccgaatcg tggtgcgtcc gtgtcaccga gtccgccacc agggaagtca gcagggtatc
      721 gatcgaccct agcagcgcga gaatgaaagc agcctggatg aaaagcggca tctcggccac
      781 cggcatctgc gggacgatga acgagggcaa cccggaagga acttcgccga tctgcggcat
      841 gccatgcagg aagaacgtcg cgacaatggt gccgatcaac aatacgccca acggcgccgg
      901 gaatcggttg tgcagcttct ttggccagta cacgacaatc gcgagcgcta ccgccgcgaa
      961 gatgaacgtg cggtaatcca cggcgccggg atccagcgtg gccaactccc tcaaagtgcc
     1021 gaggacgccg tggtccgatg ccggcaggcc caaaaacggc agggtctgga gaatcatgat
     1081 gatgacgccg atgccggtca tgaatcccga cactaccgag tacggcgtat attcgacgta
     1141 cttgccgacg cggagcagac cgaacagtat ctgcagcagg ccaccaagga tgactatggt
     1201 gaatgcttcc gcgaggctat gggaatgcgt cgcgaccacc gcgcccatca ccacggtcat
     1261 gggacccgtg ggtcccgata cctgcgccgg cgtgccgccg aacagcgcgg cgaaaaagcc
     1321 gacagctatt gcgccataga ggccggcgat cggtcctgcg cctgacgcca cgccgaacgc
     1381 gagcgccagt ggaagtgcga ccaccgctgc agtaagaccg ccgaagatgt cgttgcgaaa
     1441 cttctcccgg ccgtaggccc ccttcgctat ctgcccgatg ttcggtatga aaaggttgag
     1501 ccggcccggc gctcctgcgc ccttcgacgc gggctcgccc ggagccgacg cgcgatcatc
     1561 ctgctgcaaa atcccactcc tgaaaacgcc gccacagcgc ctcccgggcg cggcgaaacg
     1621 gcgccacaat cggactgcgc atgcattttg tcaagggacc gaggtgtttc tgcaggtaac
     1681 cgcaggggtc cgaaagacca ggaagcgata ctgattccga ttgcctcccg gacgacggat
     1741 gctcgcgagt tcggcttaag cgaactcgcc cgcatggcgg gctcccaggt catactaatg
     1801 accgataccg agctcgaggt aagacctggc gatcttttga agtgcagaca cgtacgccgc
     1861 ggtacggtag tcgggaacgt tctgacgtga gcgacggatg ccccggatct ggttgtaggc
     1921 gagccgcatg gtgtcgtcga ggcccgaacg cacgacatct atctcgcttg ccccggcgag
     1981 gagcggtgcg ctgaggtgct ccgggacggt cttccccgtc atctcctcga ccaggcggac
     2041 gatatgccgt cccctcgcct cttccaggcg ccggtcgagc cggccgaagc gtatgtgcga
     2101 tacgtttttc acccattcga aatacgagac ggttacaccg cctgcattca tgaaagcgtc
     2161 gggaaggatc acggtgccct ttttgtgcag catctggtcg gcgcgatagg tggtcggacc
     2221 gttggcggct tccgcgatca gtggagcgct gattcgatcg gcattgttct cgttgatctg
     2281 gttctcgagc gcggccggga tcaatatgtc gcaatccagt tccagcgccc ttgcggaatc
     2341 agcgatgaat tccgcatcac cgtaattggc gacgcctccg gattgccgca ggaaggcatg
     2401 cacgttttcg acgtccagtc ccttctccga aaccaacgcg ccgtctcgct cggcgatcgc
     2461 gacaatgcga acgtcgtttt cctcctgcag gaactttgcg gcgtggtatc ccacgttgcc
     2521 aaggccctgc acaaccaccc gctttcccgc aagcgttccc cgaagcccag cctctttcac
     2581 gtcttcaggg tgtcgaaaga actcctgcaa cgcatactgt attccgcgac cggtcgcctc
     2641 ggtgcgcccg gctatcccgc cttgcgtgac cggcttgccg gtcacgcagg cgatggcatt
     2701 gatgtcatcg ggaaaatcca tgcggtaggt atctgcgatc cacgccattt cccgctcgct
     2761 tgtgccgagg tctggagccg ggacgttttc gctggggctg atgtaacctt tctttgcaag
     2821 ctcgtacgca aagcgctgcg taatgacctt cagctcgtgc tcctggtagt cggcggggtc
     2881 gatctcgaga ccgcccttgg agccgccgaa gggcacgtcg acgattgcgc atttgtacga
     2941 catcagcgca gcaagggctt cgatctcgtc ctggttcacc atcttcgaat agcggatgcc
     3001 gcccttggtc ggcaggcggt gctcgctgtg gacggctcgc cagcctgtga atacctgcac
     3061 ctcgccgcgg atttcgaccg ggaacttcac ccggtagagg gagttggttt gcttgatctg
     3121 ctccgctagt ccgggaggca ggtccatggt cccgaatgcc cggtcgacca tgttgtgtac
     3181 gcttgccagg aatccgtttt tcatggcggg ggaagctcgt tgccaaggtc cgtgccattc
     3241 ttccatttaa ggaagtgcat cgccagacag gcggagtaag acgctgcctt cgctacgccg
     3301 gctgctacct aaaaacagct ttgcgcgagc aacgttcggc cggcgctgcc ggctttggaa
     3361 ttgccggcag cgcgcatagc agccaggccc tatttcctgg atgacgacct ggacgactgc
     3421 tgcgattcgc tcttgcgcga gcgaccgttg gaagagccca tgtcctcgtt cccggcggaa
     3481 cgcgattctt cgctgcctcc cgaacttccg cgctggccga cgcgcccagt gacgcggatc
     3541 cggttgtcga cgtctttcac accgcgcgcc gcatccgcga tgtcctctgc acgatgcttc
     3601 atcttcctcg atgggacggt tccggagagg gtgaccatgc catccttcac ctctatctct
     3661 atgtcctccg cgttgcaccg ggggtcggac gagagttcgt cgcagatgtc ctccttgacg
     3721 cgctcgtcgg aacgggagta gttcctggga ccgcggccgc tatagtcttc gtcgaaccgg
     3781 tggttgccgt gaccggtgcc gccctcctgc tgcccatagc cgccggaacc tccctcacgc
     3841 cggtagtcgt attgggaact gtaccgggat tcgccgggct caccggaccc gccgaggtaa
     3901 ccaaactgcg aactgccctg ctgctgtgca ccatagcccg actggtagcc gcgttgcggc
     3961 cggctgccga agccgtacct cgaacctccc tggtgcctgc cgccgtaacc ttcggactgg
     4021 tcaccgaagt agccgtattc cgacacgcgg ccgccggtac gcgagtgcgg ccagttctgg
     4081 ctgcgcggct cagaacggta gcggtcgtcc cggggccatc gagagcgtgt atcggaaaaa
     4141 ttcccggttt ccggctcgtc ccaggtatcc ctggactggc cctggcgtct cgcagcgtat
     4201 cccggctcat agtattcact gtcgtcgctg aatccctggt cgccgtagcc gtagccacgc
     4261 tccgacgcgc cgtatggatt cgactcgtcc tggctgaact cccagtccct gttgcgctcg
     4321 tcctgtcggc cgtattggct ctgccgatga tcgcgtcccc tgtcatcgtc gtattcgttg
     4381 cggggattgc ggtaggtctc ctgttcgtcg gagtaaggcc tgcgggaaga ctctcgccgg
     4441 tgctctcgtc ccgggtgacc gtggcgtctc tggtttcgtt ccatggcttt tctcctgaag
     4501 aactgctggt gttgttgcaa gaattgatcg atagactttt cgccagtgcg gtccggcatg
     4561 cgcggtcgcc atgacgatgg tttgtgcctg taactggcag caaaaaacat gccacggact
     4621 ccgtttgcgt tctcgcgaaa agctcgacga caatcgtggt gactccgtct tacgccgcct
     4681 cgatgtggca aatgcggttt gcacacatgt aaggaatgcg tccggctgta acttctaccg
     4741 gggtaaacca tggtcttgcg aggaac
//
LOCUS       DASUOM010000065         5446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_768219,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000065 DASUOM010000000
VERSION     DASUOM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768219"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /gene="mdoH"
                     /locus_tag="VFY27_01405"
     CDS             complement(<1..2148)
                     /gene="mdoH"
                     /locus_tag="VFY27_01405"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX5764195.1"
                     /translation="MGPELIADWRLPHHDALREVNPLWQAPVPRMDAYLKASGVADSD
                     RRLKIIAEIQGRLQQLLPLHQGENPLEIAMRETVQWVEDHQPVHDPAELAAVLPATPP
                     RAELAMPEQSIELRSLRWWRKRKPAGNEETYRKPGGAEGRPQQGVNDGSANSGPAQGV
                     HARRLVFASLIVATTTGAIALLSGSMQQNGLSPLEFLLVLLYAVLFAWICAAFWTAFV
                     GFIIRYFGIRRFDIDAVPLEPLPPENRPRTALIMPVYNETPRYVYARIRAIWESLQAT
                     GAGDDFDLFILSDTRDPEIQVEEELRWAKLASGIGAVGRIFYRNRVDNTYRKAGNIAD
                     FVRSWGGRYRYMAIFDADSLMEGRTLTRMVDILEAHPRVAVLQAPPVPVNNRSLYARL
                     LQFGAALYGPLYTAGMNFWQAGDASYFGHNALLRVQPFAEHCGLPSLPGQEPLGGPIL
                     SHDFVEAATLRRAGWEAWLGPSLGGSYEEIPPTLIDYAKRDRRWCQGNLQHARLVAAR
                     GWRGLTRLHFGMAVMSYLASPLWFLFLLAAGIEAWLQTRETPVYFFGHSLFPVWPISY
                     AFELMTVFLVTLFILFLPKVLALILLLAEPARWRDFGGFLRATLSAVLESLFSVVVAP
                     VLMVFQTKFVVAILMRANVGWPTQQREDRATSWGEAIRTHGSQVLLGIGAGLVTWFYV
                     PGFFWWFTPVLAGLVLAVPVSVFTSRSDVGAAAR"
     gene            complement(2133..3716)
                     /locus_tag="VFY27_01410"
     CDS             complement(2133..3716)
                     /locus_tag="VFY27_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX5764196.1"
                     /translation="MSLRRTTIKSVSLLAAVLAVLLPVPVPPALAVQAVEQAAPAAGK
                     QEPFGFENVYQRAVELAREPYQPDRPELPPVFRNMGYDQYRDIRFRPDRNLWASASLP
                     FQLQFFSRGFLYADRVTINIIDDIGPRMMPFTGDLFDFGKNQVPPRLPQNMGFAGFKI
                     LYPLHQADRLDEVAVFLGASYFRAIGQNQNYGVSARGLAIDTGLEKPEEFPVFREFWI
                     RKPGPDDTEITLFALLDSPRTTGAYRFIIQPGLQTVVDVKARVLMREDVEKLGIAPLT
                     SMFYHGENTDRFMDDFRPEVHDSDGLVIESGRGERIWRPAVNPVRLQITPFEVQSPRG
                     FGLMQRDRNYGHYQDTEAIYHTRPSAWVETRGDWGQGHVEMVEIPSGSERNDNLVAFW
                     TPAQLPKAGENIDFEYRLHFGLDPEARLQGGRTLSTRIGAGGTDVPDHSRRKFVIDFI
                     GPALKRLDPETENVEAVCNTSTGTIAQSVAHHNRFTEGWRLFFELVPEEGQAADLRCF
                     LRRGQDVLTETWVFKWAPN"
     gene            complement(3973..5187)
                     /locus_tag="VFY27_01415"
     CDS             complement(3973..5187)
                     /locus_tag="VFY27_01415"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019674917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX5764197.1"
                     /translation="MTRAIILMLDSFGIGATADAERFGDAGANTLGSIAKYRAAQGQP
                     LKVPNLARLGLLHAAKESAGEFPAGTETDAEIIGTYGFAEELSSGKDTPSGHWEMAGV
                     PVLFEWGYFRDAENTFPKELLDELIERADLPGILGNCHASGTTIIKKLGEEHVRTGKP
                     IVYTSADSVFQIACHEEAFGLERLYEMSRIARELVDEYNIGRVIARPFVGDDSSTFVR
                     TGNRRDLTVPPPEATLLDKLVESGGEVISIGKIADIYAHRGISRTIKATGNAALFDAT
                     LEALAGAPDRSLVFTNFVDFDMLYGHRRDIEGYATALEYFDRRLPELLDAMQEGDLLV
                     ICADHGCDPAWPGSDHTREHIPVLALGSGVTAGAIGKRDTFADIGQSLAGFFNLSPMA
                     YGQSFLDQSPHS"
BASE COUNT          999 a         1747 c         1726 g          974 t
ORIGIN      
        1 cctggccgcc gcaccgacgt ccgagcgact cgtgaagacg gataccggca ccgccagtac
       61 cagccctgcc agcaccgggg taaaccacca gaaaaagcct ggcacataga accaggtgac
      121 gaggccggca ccgatgccga gcaacacctg ggagccgtgg gtgcgtatcg cttcgcccca
      181 gctggtcgcc cggtcttcac gttgctgcgt cggccagccc acgtttgccc gcatgagtat
      241 tgccacgacg aacttcgtct ggaacaccat cagcaccggc gcgacgacga cagaaaaaag
      301 gctttcgagg acggcgctca acgttgcgcg caggaagccg ccgaaatccc gccagcgggc
      361 gggctcggca agcagcagta tcagcgcgag caccttcggc aggaacagga tgaacagcgt
      421 cacgagaaac acggtcatca actcgaaggc gtaggagatc ggccagaccg ggaacaggct
      481 gtgcccgaag aaatagaccg gggtttcccg ggtctgcagc caggcttcga taccggcggc
      541 gagcaggaac aggaaccaga gcggcgaggc gaggtaggac atcacggcca tgccgaagtg
      601 cagccgtgtc aggccgcgcc agccgcgcgc ggccaccagg cgcgcatgct gcagatttcc
      661 ctggcaccag cgccggtcac gtttagcgta atcgatcagg gtcggcggga tttcctcgta
      721 actgcccccg agcgatgggc ccaaccacgc ctcccatccc gcccgcctca gcgttgccgc
      781 ttccacgaag tcgtggctca gtatcggccc gcccagcggc tcctgccccg ggagagacgg
      841 caggccgcaa tgctctgcaa agggctgcac gcgcagcagt gcgttgtggc caaaatagct
      901 ggcatcaccg gcctgccaga agttcatgcc cgcggtatag agcggaccgt agagcgccgc
      961 tccgaactgc aggagcctcg cgtacagcga tcgattgttg accggcaccg gcggcgcctg
     1021 cagcaccgcg acgcggggat gtgcttccag gatgtcgacc atccgcgtga gcgtacggcc
     1081 ttccatcagg ctatccgcgt cgaaaattgc catgtagcga tacctgccgc cccacgaacg
     1141 cacgaagtcc gcgatgttgc cggccttgcg ataggtattg tcgacgcggt tcctgtagaa
     1201 gatgcgcccg acggcgccga tgccggaagc aagcttcgcc caacgcagct cctcctcgac
     1261 ctggatttcc gggtcgcggg tgtcgctcag gatgaacagg tcgaaatcgt cgcccgctcc
     1321 ggtcgcctgg agcgactccc agatcgcgcg gatgcgcgca tagacgtaac gcggcgtctc
     1381 gttgtacacc ggcatgatca gcgcggtgcg cgggcggttc tccggcggca gcggctctag
     1441 cggaacagca tcgatgtcga agcgacgaat gccgaagtag cggatgatga acccgacgaa
     1501 tgctgtccag aaggctgcgc agatccaggc aaacaatacg gcatagagca ggaccagcag
     1561 gaactcgagc ggactgagac cattctgctg catggaacca ctcagcagtg cgattgcgcc
     1621 ggtggtcgtt gcgacgatga gggacgcgaa cacgaggcgg cgcgcgtgga cgccctgcgc
     1681 gggaccggaa ttcgcggaac cgtcattaac gccctgttgc ggacggccct ccgcaccgcc
     1741 tggcttacgg taggtctcct cgttgcctgc tggtttacgc ttgcgccacc atcgaaggct
     1801 gcggagctcg atgctttgct ccggcatcgc aagttcggcg cgtggcggtg tcgccggcag
     1861 cacggccgcc agttctgccg ggtcgtgcac cggctggtgg tcctcgaccc actgcacggt
     1921 ttcgcgcatg gcgatttcca gcgggttctc gccctggtgc agcggcagta attgctgcag
     1981 ccttccctgg atctccgcga tgatcttaag cctccggtcg gaatctgcca caccggaagc
     2041 tttgagatag gcgtccatgc gcggcaccgg agcctgccac agcgggttga cttcccgcag
     2101 cgcgtcgtgg tgcggcaacc gccagtctgc gatcagttcg gggcccattt gaaaacccag
     2161 gtctcggtca atacatcctg gccgcgccgc aggaagcagc ggaggtccgc cgcctggcct
     2221 tcttccggga ccagctcgaa aaacaagcgc cagccttcgg tgaaccggtt gtggtgcgcg
     2281 accgactgcg cgatcgtgcc cgtcgaggta ttgcacaccg cctcgacgtt ttcggtctcc
     2341 ggatcgagac gcttcagcgc cgggccgatg aagtcgatga cgaacttgcg gcgcgaatga
     2401 tcgggcacgt cggtaccgcc cgcgccgatg cgcgtggaca gcgtgcgccc tccctggagc
     2461 cgtgcctcgg ggtccagccc gaagtgcagc cggtactcga agtcgatgtt ttcgcccgcc
     2521 ttgggaagct gcgccggcgt ccagaaggcg accaggttgt cgttacgttc gctgccgctt
     2581 gggatttcca ccatctcgac atgtccctgg ccccagtcgc ctctcgtctc tacccaggcg
     2641 ctcggccggg tgtgatagat cgcctcggta tcctggtaat gaccgtaatt ccggtcgcgc
     2701 tgcatcaggc cgaagccgcg cgggctctgt acttcgaacg gcgtgatctg cagtcgcacg
     2761 gggtttaccg ccgggcgcca gatgcgctcg ccgcgtccgc tctcgattac gaggccgtcc
     2821 gaatcgtgca cctccggccg gaaatcatcc atgaaacggt cggtgttctc gccgtggtag
     2881 aacatgctgg tgagtggtgc gatgcccagc ttctcgacgt cttcccgcat cagcacccgc
     2941 gctttcacgt cgacgaccgt ctgcaagccg ggctgtatga tgaagcggta ggccccggtt
     3001 gttcgcgggc tgtcgagcag cgcgaacagg gtgatctcgg tatcgtccgg acccggtttc
     3061 ctgatccaga actcgcggaa caccgggaat tcctcgggct tctccagtcc ggtatcgatc
     3121 gccagcccgc gcgcggagac gccatagttc tggttctggc cgatggcgcg aaaataactc
     3181 gcgcccagga acaccgcgac ctcgtcgagc cgatcggcct ggtgcagggg atagagaatc
     3241 ttgaagccgg caaagcccat gttctgcggc agccgcggag gcacctggtt cttgccgaaa
     3301 tcgaacaagt cgccggtaaa cggcatcatg cgcggcccga tgtcgtcgat gatgttgatc
     3361 gtcacccggt cggcgtacag gaacccgcgt gaaaaaaact gcagctggaa cggcagggac
     3421 gcactggccc acaggttccg gtccggcctg aagcggatat cgcgatactg gtcatagccc
     3481 atattccgga acacgggagg aagctcggga cggtcgggct ggtacggttc gcgggccagt
     3541 tccaccgcgc gctggtacac gttctcgaag ccgaacggtt cctgcttccc ggccgcaggc
     3601 gcggcctgtt ccacggcctg caccgcgagc gcgggcggca ccggcacggg cagaaggacc
     3661 gcgagtacgg cagcaagcag gcttacggac tttattgttg ttcgccttag agacacgcac
     3721 atcctccttg agctgagcag tacatgaaaa gcaataattt gatcagcagg cccggacgaa
     3781 ggtttcgccg catgctgccc ggggggcgcg catggccatg ccatatgcag gaataaagga
     3841 ctgtagagag tcgaactgtt taaaggggac agatctcttt ttcgctggcg gaacgcgctc
     3901 ttgcagggag gtgaaaaata gatctgtccc ctttaagcag ttcggcaggc gctcgaaact
     3961 tcgcctagtt aatcaggaat gcgggctttg gtccagaaag ctttgaccgt atgccattgg
     4021 cgacaggttg aaaaagcctg caaggctctg gccgatgtcg gcaaaggtat cgcgtttgcc
     4081 gatggcgccc gcggtcacgc ccgagccgag ggcgagcacc gggatgtgct cccgggtgtg
     4141 gtccgagccg ggccaggccg ggtcgcagcc gtggtccgcg cagatgacga ggagatcgcc
     4201 ctcctgcatc gcgtccagca gctcgggcaa gcggcggtcg aagtactcca gcgccgtcgc
     4261 atacccttcg atgtcgcggc gatggccgta gagcatgtcg aagtcgacga agttggtgaa
     4321 gacgagcgac cggtccgggg cacctgccag ggcttcaagg gtcgcatcga acaacgcggc
     4381 attgccggtc gccttgattg tccggctgat gccgcgatgc gcatagatgt cggcgatctt
     4441 gccgatgctg atgacttcac cgccgctctc gaccagcttg tccagtagcg tcgcctccgg
     4501 tggcggcact gtgaggtcgc gccggttccc ggtgcgcacg aacgtgctgc tgtcgtcgcc
     4561 gacgaacggc ctcgcgatga cgcgaccgat gttgtactca tccaccagct cgcgcgcgat
     4621 cctgctcatc tcgtagagac gctccaggcc gaacgcctct tcatgacatg caatctggaa
     4681 cacgctgtcc gccgacgtgt acacgatcgg cttacctgtt cgcacgtgct cctcgcctag
     4741 cttctttatt atcgtcgtcc cggacgcatg gcagttgccc agtatccccg gcagatccgc
     4801 ccgctcgatc agctcatcga ggagctcctt cggaaacgtg ttctccgcat ccctgaagta
     4861 gccccattcg aacagcaccg gcacgcccgc catctcccag tgaccgctcg gcgtgtcctt
     4921 cccgctgctc agttcttcgg caaagccgta cgtcccgata atctcggcgt ccgtttcagt
     4981 gccggccggg aattcgccag cgctttcctt tgccgcgtgc aacaggccca ggcgcgccag
     5041 gttcggcacc ttaagcggct gtccctgtgc cgcgcggtac ttcgcgatgc ttcccagcgt
     5101 attggcgccg gcatcaccga agcgttcggc gtcggcggtg gcgccgatgc cgaaggagtc
     5161 gagcatgagg atgattgccc gcgtcatcgt tatgccatcc ttccagtcat cgcacgacag
     5221 caggggtgct caggctgcga tgcggtgccg gtcggcctct gcctccggga gcaaggagag
     5281 tcgaagacgg tcggagtagc ttccgacggt cggaggcatc ggtgggatgg gggctgtctt
     5341 tggcggaaat aaagcggacc gaagggagcc attccgccaa agataccccc catcccgccg
     5401 gtgccctcag gcagcagccg cacagcgcac tcctcaacca cctgcc
//
LOCUS       DASUOM010000066         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_523565,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000066 DASUOM010000000
VERSION     DASUOM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523565"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..557)
                     /locus_tag="VFY27_01420"
     CDS             complement(108..557)
                     /locus_tag="VFY27_01420"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5764198.1"
                     /translation="MLAIGTRAPEFVLPNELGEDIALSSLLRDGALVLYFYPADFTPF
                     CTKEACSIRDMHPGIVQAGLRVAGVSPQDVASHLRFKERYDLPFMLLSDPEKVAARLY
                     DVDGPFGVRIRRATYLIGGDGIIRDALLADLKVSRHEEFIRKAMAAR"
     gene            complement(577..1044)
                     /locus_tag="VFY27_01425"
     CDS             complement(577..1044)
                     /locus_tag="VFY27_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5764199.1"
                     /translation="MEEFIIRDARPSDATTIVEFNMKMAMETEGRSLDPGLINPGVAA
                     VLADPGRGRYWVAEMDGQVVGQLMITCEWSDWRNGVFWWIQSVFVREDCRRKGVFSAL
                     HRHVETLARSAGEVCGIRLYVELENRRAQKTYEALGMTSPGYQVMEIDFRKGQ"
     gene            1165..1241
                     /locus_tag="VFY27_01430"
     tRNA            1165..1241
                     /locus_tag="VFY27_01430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1199..1201,aa:Arg,seq:cct)
     gene            1517..>3942
                     /locus_tag="VFY27_01435"
     CDS             1517..>3942
                     /locus_tag="VFY27_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764200.1"
                     /translation="MSHFYLSPLSGLQRLPNLGFSRRFGPLVLPVTVTVIAASLATGL
                     PAHAQQQIEEIIVTSTKRETTVQDVPFSINVQTQQDIQRSGASNLEDLSRNIAGLTVQ
                     NLGPGQSQVAIRGVSAGQIVRDQPGVKEQVGVYLDESVISLSLFTPDLDLYDLNRVET
                     LRGPQGTLFGSGSVGGTIRYITNQPNVDAFDASVELDVNTVTDGEVGGHLKGMVNVPF
                     ADGMAALRAVGYTTEFGGFIDARHEGGGITEDVNSGSRTGARVSLAFMPTDTLTITPR
                     VVYQEVETDGFNRQEVYNLFANPYTSTRPPVQLGEREQHLLLDEEFSDETLLGDVVVE
                     AAFDAFALTLTGSYLERDILVSRDASALTGSVSVDVGMPEAGILLPSNLRDTTELEQT
                     TFEARVSSVIDGRWQWLFGAFLSDTKREYRQRLPTPGYAAFLDAAVGAGTSAGAANGY
                     DLDSPYNADLPYDIEQFAFFGEVSYDVTDMLTMTVGGRYYDFDETRTITQGGFFSSGF
                     DGEVDNTSSDGFTPRVLASLALSDTMTINAQVSQGFRLGGVNDPINLTLCSDEDEVIF
                     SPFQRYGDEKMWNYETGIKSSFANGVTLNAAVFYADIEDLQVTLDAGSCSSRVSFNVP
                     DAHAAGIEFELGAQPTEAFELSLAGSFLEAEFDSTVTDALGSVLGGVEDGNRLASVPE
                     FQLAATGTFYFPLAFAGGSEAYVSATVHHVGDRITQPTDQVPGAGDFVSGLPFGGATG
                     NEVTSLDLVLDAYTIGNVSAGIIRDAWEAVLYINNVTDENADLSFDRERGGRARLAFR
                     TNQPRTIGLT"
BASE COUNT          766 a         1259 c         1170 g          747 t
ORIGIN      
        1 ccgaaggcca ccgatgttag ggccacgcct gacgaaattt cgtggaatcc cattccaaaa
       61 gatctcgtcg catgagactc ggacgagttc gccaccgtag atcttcccta tcgtgctgcc
      121 atcgccttgc ggataaattc ctcatgccgg ctgaccttga ggtccgccag cagggcatcg
      181 cggatgatcc cgtctccgcc gatcaggtaa gttgcacgcc ggatgcgcac gccgaacggg
      241 ccgtccacgt cgtacaggcg ggcggcaacc ttctccgggt cgctcaacag catgaacggc
      301 aggtcgtacc gctctttgaa acggagatga ctcgcgacgt cctgcggact cactcccgcc
      361 actctcagcc ctgcctgcac gatgcctgga tgcatgtcgc ggatggagca ggcttccttt
      421 gtacagaatg gcgtgaagtc cgcgggataa aaatacagaa ccagcgcacc gtcgcgaagc
      481 aggctggaga gcgcgatgtc ctcgccaagc tcattcggca gcacgaattc cggcgccctg
      541 gtgccgattg caagcaacgt tttctcccgg cagcgtctac tgccctttcc ggaaatcgat
      601 ctccatgacc tggtaacccg gcgaggtcat gcccagcgct tcatatgtct tctgcgcacg
      661 ccggttttca agctcgacgt agagccggat cccgcaaacc tcgccggcag accgcgcaag
      721 cgtttccacg tgacgatgca aggcggaaaa gacgcccttg cgccggcagt cttcgcgaac
      781 gaagacgctc tggatccacc agaaaacccc gttccgccag tcgctccatt cacaggtgat
      841 catgagctgt ccgaccacct ggccatccat ctcggcaacc cagtaccggc ccctgcccgg
      901 atccgcgagc acggccgcga caccggggtt gatcagcccg gggtcaagcg aacggccctc
      961 ggtctccatc gccatcttca tattgaactc gacgatcgta gtcgcgtcgg agggacgcgc
     1021 gtcacgaatt atgaattcct ccatctgtcc tgcatccgca tccggaacgt tccgggacaa
     1081 tacaagcctc gagccacttt gcctaccgca tcaccgtgcg ccggaaatgc ggcgccaaat
     1141 cgcttagaat cctccaccag tgaagccccg gtagctcagt aggatagagc accagattcc
     1201 taatctgggg gtcccatgtt cgaatcatgg tcggggcgcc atccacaagg tgaccgcagg
     1261 tcgccttgtg gatggcggct cggcctgctg atgagaacat tgttcgacaa atcggcagga
     1321 gccgatttgg accgcgaagc gacccgagcg gagcgagggc gaggtccacg gagaccgagt
     1381 caatcatggt cggggcgcca tcttcgttgt aactgcgcta caccagtcat ttcggcgtag
     1441 gcggtgccac tcgcactcat ttgcgtataa tcggcccggg gggaggttct caagcaacag
     1501 ccacacgggg accgccatgt cgcattttta tttgtcgccg ctctcgggat tgcagcgcct
     1561 tcccaacctc gggttttccc gccgtttcgg gccacttgtc ctgcccgtaa ccgttaccgt
     1621 catcgcggcg tcgctcgcaa ccggcctgcc ggctcacgcg cagcagcaga tcgaggagat
     1681 catcgtcacc tcgaccaagc gcgagaccac cgtgcaggac gttccttttt cgatcaacgt
     1741 ccagacacag caggatatcc agcgttccgg cgcctcgaac ctcgaagacc tttcgaggaa
     1801 catcgccggg ctgacggtgc agaacctggg gccgggtcag agccaggtcg cgattcgcgg
     1861 cgtgtccgcg ggacagatcg ttcgcgacca gcccggcgtg aaggaacagg tcggcgtcta
     1921 tctggatgag tccgtcatct cgctgtcgct gttcacgccg gacctcgacc tgtacgacct
     1981 caatcgcgtc gagacactcc gtggaccgca aggcacgctc ttcggatccg gatcggtagg
     2041 cggcacgatc cgctacatca cgaaccagcc gaacgtggac gcgttcgatg ccagcgtcga
     2101 attggacgtg aatacagtga cggacgggga ggtcggtggc cacctcaaag gaatggtgaa
     2161 cgtgccgttt gcggacggga tggcagcgct acgggctgtc ggctacacca cggagttcgg
     2221 cggctttatc gatgcgcggc acgagggcgg cggcatcacc gaggacgtca acagcggcag
     2281 ccgcacgggt gcccgggtgt ccctggcctt catgccgacc gacacgctga ccatcactcc
     2341 gcgcgtcgtg taccaggagg tggaaacgga cggcttcaac cgccaggagg tctacaacct
     2401 tttcgccaat ccctacacat cgactcgccc gccggtacag ctcggggaac gtgaacagca
     2461 cctgctgctc gatgaggaat tttcggatga aacgctgctt ggcgacgtcg tcgtcgaagc
     2521 cgcattcgat gcgttcgcac tcacgctcac cggcagctac ctcgagcgtg acatcctggt
     2581 cagccgtgat gcgagtgcgc tgaccggcag cgtatccgtc gatgtgggga tgccggaagc
     2641 cgggatcctc ctgccttcca acctgcgcga cacgacggag ctggagcaga cgacgttcga
     2701 agccagggtg agctccgtga ttgacggccg gtggcagtgg ctgttcggcg cgttcctctc
     2761 ggataccaag cgggaataca ggcaacgttt accgacaccc ggatacgctg ctttcctgga
     2821 tgcagcggtg ggcgcgggca cttcggcggg cgcggcgaat ggctacgacc tggattcgcc
     2881 ttacaacgcc gacctgccgt acgacatcga gcagttcgcc ttctttggcg aagtgtccta
     2941 cgacgtcacc gacatgctca cgatgacggt cggcggccgc tactacgact tcgacgagac
     3001 ccggaccatc acgcagggcg gcttcttctc atccggcttc gacggcgaag tcgacaacac
     3061 ttcgtcggac ggcttcacgc cgcgcgtact cgcgagcctc gccctgagcg atacgatgac
     3121 catcaacgcg caggtttcac aggggttccg cctcggcggc gtgaacgatc caatcaacct
     3181 gacgctgtgc tcggacgagg acgaggtgat attcagcccg ttccagaggt atggcgacga
     3241 gaagatgtgg aactacgaaa cgggcatcaa gtccagcttc gccaacggcg taacgttgaa
     3301 tgccgcggtg ttctacgccg acatcgagga tctgcaggtc accctcgatg ccggatcgtg
     3361 ttcatcgcgc gtatctttca acgttcccga tgcccatgca gcgggcatcg aattcgagct
     3421 gggtgcgcag ccgaccgaag cattcgaact gtcgctggcc ggtagcttcc tcgaagcaga
     3481 attcgactcg accgttaccg acgcgctcgg ctccgttctt ggtggagtgg aggatggaaa
     3541 tcgcctcgcc tctgttccgg aattccagct tgccgctacc ggtacattct acttcccgct
     3601 ggcgttcgcc ggaggatcag aagcgtatgt ttccgcgacg gttcaccacg taggcgaccg
     3661 gataacccag cccacggacc aggtgccggg agccggcgat ttcgtctccg gcctgccgtt
     3721 cggcggcgcc accggcaacg aggtcaccag cctggatctc gtgctcgatg cctatactat
     3781 cgggaacgtg agtgcaggca taatcaggga tgcgtgggag gcggttctct atatcaacaa
     3841 cgtcactgat gagaacgccg acctttcgtt cgatcgcgag cgcggcggcc gcgcgcgcct
     3901 ggccttccgt acgaaccagc ctcgtaccat tggcctgaca tt
//
LOCUS       DASUOM010000067         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_701688,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000067 DASUOM010000000
VERSION     DASUOM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701688"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFY27_01440"
     CDS             <1..1572
                     /locus_tag="VFY27_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX5764201.1"
                     /translation="MSELRVSKPFRQPAERIRNIVVVGGGTAGWMAAAAFSQVLPREL
                     VSIRLIESDDIGRVGVGEATIPPICTFNAMLRIDENDFLRRTQGTFKLAIELTDWTRI
                     GHSYFHPFGEFGLDIEGVQFHQFWRKLHLRGEVPGIEEYCVPAMAARHNKFARPATDP
                     ASVASRLKYAYHFDASLYAALLREYAEARGVTRTEGKILDVKLRAEDGFVEAVNLEGG
                     ATITADLFIDCSGFRGLIIEEALKAGYEDWSHWLPNDSALAIQCESHGELTPYTRSTA
                     RAAGWQWRIPLQHRVGTGYVFSSRYVSDDEAAATLMANLESPPLTDPRLLRFTGGHRK
                     KFWSRNCVALGLASGFLEPLESTSIHLVQVGITRLLALFPDMSFNPVEIEQYNRFMTS
                     QYETIRDFLVLHFKATERSDSDYWNYCRMMDVPDSLRHKMELFRAQGRVFRYEDELFA
                     EANWIAVLLGQNVLPESYDTLADRLDTAAVVRYLEHIRTAIKQTVDAMPTHRGYVRKN
                     CAAEPVAAVPDAAAG"
     gene            1576..>2324
                     /locus_tag="VFY27_01445"
     CDS             1576..>2324
                     /locus_tag="VFY27_01445"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX5764202.1"
                     /translation="MDKRLRSIVIVGGGTAGWMTAAALSRFLTDGYTKVRLIESEEIG
                     IVGVGESTIPLMRTFNRMLGLDEDEFMRKTRGTFKLAIEFVDWTRLGHVYHHPFGPAG
                     LNMDGVSFHAYWLRMNELGEAPDLGEYSLQTVAARQEKFSRPVDAGNSPLSTIAYAFQ
                     FDASLYAQYLREYAEARGTERTEGQIVDVGLRGEDGFIEAVTLESGARVEGDLFIDCS
                     GFRGLLIEQALKTGYEDWSHWLPCDRAVATQ"
BASE COUNT          450 a          746 c          724 g          404 t
ORIGIN      
        1 atgagcgaac ttcgcgtaag caaacctttc cggcaaccag ctgagcgcat tcggaacatc
       61 gtggtcgtcg gcggcggcac ggccggctgg atggcggctg ccgcgttctc gcaggtgttg
      121 ccgcgtgagc tggtgagcat ccgcctcatc gagtcggacg atatcggccg cgtcggcgtt
      181 ggcgaagcga ccataccgcc gatctgcacg ttcaacgcaa tgctgcgcat cgacgaaaac
      241 gatttcctgc gcaggacgca aggcaccttc aagctggcga tcgagctaac cgactggacc
      301 cgcatcggcc actcatattt tcacccgttc ggcgagttcg gcctggacat cgagggcgta
      361 cagtttcacc agttctggcg caagctgcac ctgcgcgggg aggtgccggg aatcgaggag
      421 tattgcgtgc ccgcgatggc ggcgcgacac aacaagtttg ctcgccccgc taccgacccc
      481 gcctcggtcg catcacggct gaaatacgcc tatcacttcg acgcgtccct ctacgccgcg
      541 ctgctccgtg aatacgccga agcacgtggc gtcacccgca ccgaagggaa gatcctggac
      601 gtgaagctgc gcgccgaaga cggcttcgtc gaagccgtca atctcgaagg cggcgcaacc
      661 atcaccgctg acctcttcat cgactgcagc ggcttccgcg gtctcatcat cgaagaagcg
      721 ctcaaggccg gctacgagga ctggtcacac tggctgccga acgacagcgc gctcgccatc
      781 cagtgcgaaa gccacgggga actgaccccc tatacgcggt cgaccgcgcg cgctgccggc
      841 tggcaatggc gtattccgct gcagcaccgg gttggcaccg ggtacgtctt cagcagccgc
      901 tacgtgagcg acgacgaggc ggcggcaacc ctgatggcaa acctggaaag cccgccactc
      961 acggacccgc gtctgctgag gttcaccggt gggcaccgca agaagttctg gagccgcaat
     1021 tgcgtggcgc tcggcctcgc gagcgggttc ctcgagccac tcgagtcgac cagcattcac
     1081 ctcgtccagg tgggtatcac gcgcctgctg gcgctgtttc cggacatgag cttcaacccg
     1141 gtcgagatcg agcagtacaa ccgcttcatg accagccagt acgagaccat ccgcgatttc
     1201 ctcgtgctgc atttcaaggc gacggaaaga agcgattcgg actactggaa ctactgccga
     1261 atgatggatg tccccgattc gctgcggcac aagatggagc tattccgggc gcagggtcgg
     1321 gtgttccgct acgaagacga gctgttcgcg gaggcgaact ggattgcggt gctgctgggg
     1381 cagaacgtgc tgcccgagtc ctacgacacg ctggccgaca ggctcgacac cgccgcggtc
     1441 gtgcggtacc tcgaacatat caggacagcc atcaaacaga ccgtggacgc catgcccacc
     1501 caccgcggct acgtccggaa gaactgcgcc gccgaacccg tggcggccgt accggacgcg
     1561 gcggcaggct gagacatgga taagcgcctg cgcagtatcg taatcgtggg aggcggcacc
     1621 gccggctgga tgaccgcggc cgcgttgtcg cgattcctca cggatggcta caccaaagtg
     1681 aggctgatcg aatcggaaga gatcggcatc gtgggtgtcg gcgagtcgac catccccttg
     1741 atgcgcacct tcaaccgcat gcttgggctc gacgaggacg agttcatgcg gaagacccgg
     1801 ggaaccttca agcttgccat cgaattcgtc gactggacac gcctggggca cgtttatcac
     1861 cacccgttcg gcccggccgg cctgaacatg gacggcgtct cctttcacgc ctactggctg
     1921 cgcatgaatg aactgggcga ggcgcccgat ctcggcgagt actcactgca gacggttgcc
     1981 gcacgccagg agaaattcag tcgcccggtg gatgccggca actcccccct ttccaccatc
     2041 gcgtacgcgt tccaattcga tgcatccctt tatgcgcaat accttcgtga atatgcagag
     2101 gctcgcggaa ccgagcgtac cgagggccag attgtcgacg tcgggctgcg gggagaggac
     2161 ggattcatag aagcggtaac cctcgaaagc ggcgcgcgcg tcgagggcga cctgttcatt
     2221 gactgctccg gcttccgcgg gctgctcatc gagcaggcgc tgaagaccgg ctacgaagac
     2281 tggtcgcatt ggctgccctg cgatcgcgcc gtcgccacgc aatg
//
LOCUS       DASUOM010000068         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_493833,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000068 DASUOM010000000
VERSION     DASUOM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493833"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VFY27_01450"
     CDS             <1..1437
                     /locus_tag="VFY27_01450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5764203.1"
                     /translation="VMVLSLIGRIVLRGGYHSGAVYSRRNRNAPVIMAVGVGLVILGW
                     IGVLSARIIKSAVSRQRETLADASAVQFTRQTQGLANALKKIGGYAEKSYLRAADPEE
                     VSHMLFARGSRLASLFATHPPLVQRIQALDPTFREEDFPRIVPQPQGASQMSQAMGFA
                     DTPAGSGATEFDVAGYAARDGRSIADSVGNPGNRQIAFAEALRQSIPDALYGAAHGEA
                     TALLLTLALVLDRSGRHVDRQLHLVAEQFGGERTSVVKRYYEELLKAGPQFSLPLLEI
                     SFPALKQRPAAQLQFLLDIVRRLVETDGEVDLFEYCFYRVLVASLDPVGVPFRSRAGR
                     KLSRRPARQAAVQLLGIVANFGHDSPPSREAAFRAGAGLLGAWAANTSDESRSGLPAA
                     QDRVAELDKCLDLLRGLGMSAKERLLHALSAVVGHDRRLTVVESELMRAICAGLDCPL
                     PPLLIENPIAPTQLGKPGEQARPRDVAE"
     gene            1657..2505
                     /locus_tag="VFY27_01455"
     CDS             1657..2505
                     /locus_tag="VFY27_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX5764204.1"
                     /translation="MRIRLPFSIILLSLTVAMPAAAATPEEERVAAATDVIDQLLRIP
                     EQSVPDVLLSRAYAVAVIPNVVKIGFGLGARRGKGVLVVRQDNGTWSNPAFVTLTGGS
                     FGWQIGAQSTDIILVFKTREGVDDISKGKLTLGADASIAAGPVGRHTGIATDIEFQAE
                     VFSYSRSRGLFAGVALEGAGVTMDRGANAAFYGSSTITPEQIFASSGNSAPAVANNFV
                     QVLSAETRQLPAQPGMDSDGDPPGSSMDNRDSAAPTPESSPAVRTYGIGDPDAADAED
                     EQYDNF"
     gene            complement(2551..3183)
                     /locus_tag="VFY27_01460"
     CDS             complement(2551..3183)
                     /locus_tag="VFY27_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764205.1"
                     /translation="MKRTVALFFVLSLPGACGKELPQPTVAEFMEDPILLEATIVRCG
                     EDRSATRYDAACVSARDAVDRIAAAREQERRMELEAESERKRQALRRTQEAAAESRRL
                     AEEAEKRREEAAYYGEFEPLPAEVETSEERPAAGEDETSNAAGTGILKDTPLPGNSRP
                     ALPDDGVVRMPVVTESEPGVSTGIETEAPAAAEPDAVPEELKRRQDEGQE"
     gene            3368..>4701
                     /locus_tag="VFY27_01465"
     CDS             3368..>4701
                     /locus_tag="VFY27_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5764206.1"
                     /translation="MSETNQFSLLRQRRFAPFFLTQFLGAFNDNIFRNGLVILITFQG
                     VRIANMDAQLLANVAGALFILPFFLFSATAGQLADKHEKSLLIRRIKLLEIALMVLAA
                     TAFVAGSYLTLLLVLFLMGLQSTLFGPVKYAYLPQQLETSELVGGNALVESGTYMAII
                     LGLMAGGIAVAANQGQHVILSVCLVAVAVAGYVASRGIPVTRAVDPGLRINWNAWTET
                     SRIVGFARERRSVFLSILGVSWFWFFGSALTFQIPAYTLNVLSGSEEITTVLLVAFAV
                     GVGGGSLLCEKMSAHRIELGLVPFGSIGLSVFAIDLYFAQPVVHPVSLHSVGEFLGRP
                     GSFRVLADLALLGAFGGLYSVPLYAMIQERAERQHLSRIIAANNILNALFMVCAAILA
                     ISLLNSGVSIPQFFLVLALLNALVAIYIYTLLPEFLIRFLAWILINTLYRIRA"
BASE COUNT          809 a         1500 c         1427 g          965 t
ORIGIN      
        1 gtgatggtgc tgagcctcat cgggcgcatc gtcctccgtg gcgggtatca cagcggcgcg
       61 gtatacagcc gccggaaccg caacgctccc gtcatcatgg ccgtcggcgt gggcctggtg
      121 atccttggct ggatcggcgt cctctccgca cggatcatca agagcgcggt ttcacggcag
      181 cgcgagacgc tcgcagatgc atcggcggtg cagttcacgc ggcagaccca ggggctcgca
      241 aacgcgctca agaaaatcgg ggggtacgcg gaaaagtctt acctgcgcgc cgccgatccg
      301 gaagaggtca gccacatgct gtttgcgagg ggcagccggc tcgcgtctct ttttgcgacg
      361 catccaccgc tcgtccagag aatccaggcg ctcgacccga cgtttcgcga ggaggatttt
      421 ccgcgcatcg tgccgcagcc gcagggcgcc tcgcagatgt cgcaggcgat gggcttcgcg
      481 gatacccctg caggttccgg agcgacggaa ttcgatgtgg ccggatacgc agcaagagat
      541 ggccgctcga tcgccgattc ggttggtaat ccgggaaatc gccagatagc gttcgccgag
      601 gcgctccggc agtcgattcc cgacgccctc tatggtgcag ctcacggtga ggccaccgca
      661 cttctcctga cgctcgcgct ggtgctcgac cgcagcgggc ggcatgtcga ccgccagctt
      721 cacctggttg cagaacagtt cggaggcgag cgcacgtctg tcgtaaagcg ctattacgag
      781 gagttgctga aggcaggacc gcagttcagc ctgccgttgc tcgagatcag ttttccggcg
      841 cttaagcagc gaccggcggc gcagctgcaa ttcctgctcg acatcgtgag gcggctcgtt
      901 gaaacggatg gcgaagtcga cctgttcgaa tactgcttct accgtgtact cgtcgcgagt
      961 ctcgacccgg ttggtgttcc gttccgtagc agggccggcc gcaagctgtc gaggcgcccg
     1021 gcgcggcagg cagcagtgca actattgggc atcgtcgcga atttcggcca tgacagcccg
     1081 ccaagtcgcg aagcggcgtt ccgagccggc gccgggctcc tcggggcctg ggccgccaac
     1141 acgagcgatg agagccgttc aggcctcccg gccgctcagg atcgcgtggc cgaactcgac
     1201 aagtgcctcg acctgctgcg gggcctcggc atgtcggcga aggagcgact gcttcatgcc
     1261 ttgagcgccg ttgtcggtca cgaccgaagg ctgacggtcg tggagagcga gctgatgcgt
     1321 gccatctgtg caggcctcga ctgccctctt ccgccgctgc tcatcgaaaa cccgattgcg
     1381 ccaacgcaac tcggcaagcc aggcgaacag gctcggcccc gtgatgttgc cgagtgaagc
     1441 gttgacgagt tgacgattct cgctcaaacg agcgcgcggg tacttgcggc cgcacaattg
     1501 gcgtatcatg tgccgcggtg taacccgcgg gagaaaccga ctctccttat gcacgaccct
     1561 tcacgatact ggtcacgaca cttattacga cgagcagggc ggctcttcga cgtatcctgt
     1621 caggactcca gaaatcgcca accttcggta actatcatgc gcatacgcct gccattctcg
     1681 atcattttgc tgtcactcac cgtcgcgatg cctgccgcag ccgcaacccc tgaagaagag
     1741 cgcgttgccg cagcgacgga cgtcatcgac cagctgctga ggattccgga acaatcagtc
     1801 cccgatgtgc tgctttcccg ggcgtatgct gtcgcggtga taccaaatgt ggtcaagatc
     1861 ggcttcgggc tgggtgcaag acgcggcaag ggagtactgg tcgtccggca ggacaacgga
     1921 acctggagca accccgcctt cgtcacgctg accggaggca gcttcggttg gcagatcggg
     1981 gcgcaatcca ccgatatcat cctcgtattc aagacgcgcg aaggcgtcga cgacatctcg
     2041 aaaggcaagc tgaccctggg cgcggatgcc tcgatcgccg cgggaccggt cgggcggcat
     2101 acgggcatcg ctaccgacat tgagttccag gcggaggtat tttcctactc gcgcagccgc
     2161 gggttgttcg cgggggtcgc gctcgagggg gcgggagtca ccatggatcg cggcgcgaac
     2221 gcggcattct acggctcatc gaccattaca cccgaacaga tcttcgcaag ctccggcaac
     2281 tcggctccgg ccgtcgccaa caatttcgtg caggtattat cggccgagac acggcagctg
     2341 cccgcgcagc ccggcatgga ttccgacggg gaccctccag gttcttcgat ggataaccgc
     2401 gacagcgccg cccccactcc ggagtcttct cctgcggtcc gcacctatgg cattggcgat
     2461 cccgatgcgg cagatgccga agacgagcaa tacgacaact tctagtcact atgtgactgg
     2521 tctctgactc aggacttcag gcttccgggg ttattcctgc ccttcgtcct ggcgacgctt
     2581 cagttcttcg gggacagcat cgggttctgc cgccgccggc gcttcagttt cgattccggt
     2641 gctcacgcca ggctcgcttt cggtaaccac aggcatacgc accacgccgt catccggcag
     2701 cgccggccgc gagttccccg ggagcggggt gtccttaaga atgccggtgc ccgcagcatt
     2761 gctggtctcg tcctctccgg ccgcgggtct ctcctctgac gtctcgactt ccgccggcaa
     2821 gggctcgaat tcgccgtagt aggcagcctc ctcccgccgc ttttccgctt cctccgccag
     2881 gcgcctgctt tccgcagcgg cctcttgcgt gcgcctcagc gcctgtctct tccgttccga
     2941 ctcggcctcg agttccatgc ggcgctcctg ttccctggcg gctgcgatac ggtcgactgc
     3001 gtcgcgggcg ctgacgcagg ccgcgtcata acgggtggca gagcggtcct ccccgcagcg
     3061 cacgatcgtc gcttcgagca gtatggggtc ctccatgaac tccgccaccg tgggctgagg
     3121 aagctccttg ccgcaagcgc cgggtagcga aagcacaaaa aatagcgcaa ctgtgcgttt
     3181 catcgtcgct cctgcgaccc gggtaagtaa gcttgaggtg gtcaaagtac cgcgataata
     3241 acactcagga cgttggggaa gccctgccgg gcgtcacgca ataaaaaggt cgtgaaagac
     3301 gtggtggtat agtggcgccg ccgtaacagg cgcgctgcag gagtcttttg gtcatggatg
     3361 ctgaatagtg tcagagacaa accagttctc gctcctgcgc cagcgccgct tcgctccctt
     3421 tttcctcacg cagttcctcg gagccttcaa cgacaacatc ttccgcaatg gcctggtcat
     3481 attgatcacg ttccagggcg tccgcatcgc caacatggat gcacaactgc ttgccaacgt
     3541 ggccggagca ctgttcatcc tgccattctt cctgttctcc gcaactgcag gacagctcgc
     3601 cgacaaacac gagaagtcgc tgttgatccg gcgcatcaag ttgctggaaa tcgcgctgat
     3661 ggttctcgcc gccacggcct tcgttgccgg aagttatctg acgctgctgc tcgtgctttt
     3721 cctgatgggc ctgcaatcca cgcttttcgg accggtgaag tacgcttacc tgccacaaca
     3781 actggaaaca agcgagctgg taggcggcaa tgccctggtc gagtcgggca cctacatggc
     3841 catcatcctc gggctgatgg ccggcgggat cgcagttgcg gcaaaccagg ggcagcatgt
     3901 aatcctttcc gtttgcctgg tcgccgttgc tgttgccggc tacgttgcca gccgcggcat
     3961 ccccgtaaca cgcgcggtcg atccaggcct caggatcaac tggaatgcgt ggacggaaac
     4021 ctcgcgcatc gtggggttcg cccgcgaacg tcgcagcgtt ttcctctcga tactgggcgt
     4081 ttcatggttc tggttcttcg gctcggctct gacattccag atcccggcgt atacgctcaa
     4141 cgtcctgagc ggcagcgaag agatcaccac ggttctgctg gtcgcttttg cggttggcgt
     4201 cggcggcggc tccctcctct gcgagaagat gtcggcgcac cggatcgagc tgggcctggt
     4261 gccgttcggc tcgataggat tgagtgtatt cgcgatcgat ctttatttcg cccagcccgt
     4321 cgtgcacccg gtcagcctgc attcggtcgg cgagttcctg gggcggcccg gcagttttcg
     4381 cgtgcttgcc gatctcgcgc ttctcggtgc tttcggcgga ctctacagcg tgccgctgta
     4441 cgccatgatc caggagcgcg cggaacgaca acacttgtcg cgcatcattg ccgccaacaa
     4501 catcctgaat gccttgttca tggtgtgcgc cgcaatcctt gcgatctcgc ttctcaacag
     4561 cggcgtttcc ataccgcagt tcttcctggt gctggcgctc ctgaatgccc tggtggcgat
     4621 ctacatctac acgttgcttc cggaattcct gattcgcttc ctggcgtgga tactgatcaa
     4681 tactctctac cggattcgcg c
//
LOCUS       DASUOM010000069         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_224391,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000069 DASUOM010000000
VERSION     DASUOM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224391"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFY27_01470"
     CDS             <1..598
                     /locus_tag="VFY27_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764207.1"
                     /translation="RSMRRCLEGGSLPMHRYALSLLPALVLVSLLLPVSAARGAEAPP
                     EPATAAMPPPPAWLEELDTLYDARVRVTGLDDRAFSPEHWWDVAGPLATTERGFHTEQ
                     VGRSVEGRPLRHVSWGGGNTPVLLWSQMHGDESTASMAIADLFRFLGENPQHPIVQHT
                     SGAVSISACARRLLNLALDHARDVASSAAAGPSAISPP"
     gene            complement(538..1632)
                     /gene="arsB"
                     /locus_tag="VFY27_01475"
     CDS             complement(538..1632)
                     /gene="arsB"
                     /locus_tag="VFY27_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5764208.1"
                     /translation="MTESISRKLSVLDRYLTLWIFIAMAVGVAIGYFVAGAPAFIQRF
                     DVGTTNVPIAIGLILMMYPPLAKVRYELLPKVFADKKVLGLSLAQNWLVGPALMFVLA
                     VVFLHDYPEYMVGLILVGIARCIAMVLVWNQLARGNSEYAAALVALNSLFQVITFGFY
                     AWLFVTVLPPLLGLQGSVVNVSIAEIFTSVMIYLGIPFAAGFLSRLILVRAKGRDWYQ
                     EVFLPRISPITLVALLFTIVVMFTLQGQAIIQSPLDVVLIAVPLTIYFALMFLLTFFA
                     AWRLGADYSRSTSVAFTASGNNFELAIAVAIAVFGIESKVAFATVIGPLVEVPVLIGL
                     VNVALYFQRRYYGGEIAEGPAAAEDATSRA"
     gene            complement(1629..2060)
                     /locus_tag="VFY27_01480"
     CDS             complement(1629..2060)
                     /locus_tag="VFY27_01480"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5764209.1"
                     /translation="MKKLKVLFLCTGNACRSQMSEGWARHLKGDRIEAYSAGVAPHGL
                     DPRAVKVMEEAGVDISAHSSKHLDTLLHVPFDYVVTVCDNAAESCPIFPGNVKKVHRS
                     FQDPPALARTAHSEEEALEHYRRVRDEIRTFIETLPEALTT"
     gene            complement(2077..2568)
                     /locus_tag="VFY27_01485"
     CDS             complement(2077..2568)
                     /locus_tag="VFY27_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX5764210.1"
                     /translation="MKRLHVHVAVDDLETSTRFYSQLFAADPVVAKPDYAKWMLDDPR
                     VNFAISARATRPGIEHLGIQVEDRDELAEIYQRLERAGGAVLEEGATTCCYAQSEKSW
                     IEDPQGVKWETFLTTGESTVYGSDPVSLEAASGCCKPASQGEASCCAPAKHANESSAI
                     CCS"
     gene            complement(2575..3009)
                     /gene="arsN2"
                     /locus_tag="VFY27_01490"
     CDS             complement(2575..3009)
                     /gene="arsN2"
                     /locus_tag="VFY27_01490"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEX5764211.1"
                     /translation="MEPISGQPSRQGVIALLSRAQLPTSDITDELLESFYFAGERTDP
                     LGIVGLELSRPHALLRSLVVKAEARSGGLGSRLLEHAEEQARARGAHRIYLLTTTAEP
                     FFAARGYAGANRSDAPPFIRSSAEFASLCPASAAFMVKHLQE"
     gene            complement(3018..3365)
                     /locus_tag="VFY27_01495"
     CDS             complement(3018..3365)
                     /locus_tag="VFY27_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5764212.1"
                     /translation="MNTPDVITALAALAHEHRLGIFRLLVEKGPEGLPAGEIAERIGL
                     VPSSLTFHLQHLARAGLLTQRRVGRQLFYATDFGVMNELVGYLTENCCGGAGCASAAA
                     PRGKSRRTSTRQA"
BASE COUNT          697 a         1102 c         1140 g          655 t
ORIGIN      
        1 tcggagcatg cggcgctgcc ttgagggagg atcgctgccc atgcatcgtt atgcactctc
       61 cctgctgccg gccctggtgc tggtctccct gctgttgccc gtatccgcgg cccggggtgc
      121 cgaagctcct ccggagcccg ccacggcggc gatgccgccg cctccggcct ggctcgagga
      181 actggatacg ctgtacgacg cgcgcgtccg ggtgacgggc ctggacgacc gggcgttttc
      241 gccggagcac tggtgggacg tcgccggacc cctggcaacg acagagcgcg gcttccatac
      301 cgaacaggtc ggccgcagcg tcgaggggcg accgctgcgc cacgtgtcct ggggaggtgg
      361 caacacgcct gtgttgctct ggtcgcagat gcacggcgac gagagcaccg cgtcgatggc
      421 aatcgccgac ctgttccgct tcctcggcga gaacccgcag cacccgatcg tgcagcacac
      481 atccggcgca gtatccattt cggcgtgcgc aaggagattg ttgaacctgg ctcttgatca
      541 cgctcgtgat gttgcgtctt ccgccgccgc tggaccttcg gcaatttctc ccccgtagta
      601 acgacgctgg aagtacaggg ccacgttcac gaggccgatc aggaccggca cttctaccag
      661 cgggccgatg accgtggcga aagctacctt cgattcgatg ccgaacacgg cgatcgccac
      721 cgcgatggcc agttcgaagt tgttgccgga ggcagtgaag gcaacgctgg tcgagcggga
      781 gtaatctgcg cccagccgcc aggcggcgaa gaacgtcaac aggaacatca gcgcgaagta
      841 gatggtcagt ggcacggcaa tcagtacaac gtccaacggc gactggatga tcgcctgacc
      901 ctgtagcgtg aacatcacca cgattgtaaa aagcagcgcg accagggtga tcggagagat
      961 acgcggcaga aagacctcct ggtaccaatc cctgcctttc gcgcggacga gaatcagcct
     1021 cgaaaggaag ccggcggcaa aaggaatccc gagatagatc atgacgctcg tgaaaatctc
     1081 ggcgatcgag acgtttacga cggagccctg gagccccaga agtggcggca gcaccgtgac
     1141 gaaaagccac gcatagaagc cgaacgtgat gacctggaag agactgttga gcgctacgag
     1201 cgccgcggca tactcactgt tgccgcgggc cagctggttc cacacgagca ccatggcgat
     1261 gcagcgcgcg attcccacga ggatcagccc caccatgtac tccggataat cgtgcaggaa
     1321 cacgacggcc aggacgaaca tcagcgcggg ccctaccagc cagttctgcg cgagcgacag
     1381 gccgagcact ttcttgtcgg cgaacacctt cggcaggagt tcgtaacgca ccttcgccag
     1441 cggcgggtac atcatcagga taagcccgat tgcgatcggc acgttcgtgg tgccgacgtc
     1501 gaagcgttgg atgaatgcag gcgctccggc cacgaagtac ccgatcgcca cgccgaccgc
     1561 catggcgatg aagatccaca gggtgagata gcgatccagg acggatagct tgcggctgat
     1621 cgattcggtc atgtagtcag agcctcaggc agtgtctcaa tgaaggtacg gatctcgtcc
     1681 cttacccggc ggtagtgctc aagcgcctcc tcttcgctgt gtgccgttcg ggccagagca
     1741 ggcgggtcct ggaaactgcg gtggaccttt ttgacgttgc cgggaaaaat cggacagctc
     1801 tcggcagcgt tgtcacagac cgtgaccacg taatcgaacg gcacgtggag gagcgtgtcg
     1861 aggtgtttag agctgtgggc ggagatgtcc acgcccgcct cttccatcac cttcacggct
     1921 cgcggatcga gcccgtgtgg cgccacgccg gcagagtaag cttcgatccg gtcgcctttg
     1981 aggtgacgcg cccagccctc gctcatctgg ctacggcacg catttcccgt gcacaggaag
     2041 agcaccttga gctttttcat gccgtgctcc gcacgttcag ctgcagcaga tggcgctgct
     2101 ctcgtttgca tgtttcgcgg gcgcgcagca ggaggcttcg ccctgcgaag ccggtttgca
     2161 gcatcccgat gccgcttcga ggctgacagg atcgctgccg tataccgtgc tctcacctgt
     2221 cgtaaggaac gtctcccact tcacaccctg gggatcctcg atccagctct tctcgctttg
     2281 tgcgtaacag caggtggtag cgccctcctc caatacggca ccgccggcgc gttccaggcg
     2341 ctggtagatt tccgcgagtt catcgcggtc ctcgacctgg atgccgaggt gctcgatgcc
     2401 cggccgtgta gctcgtgcgc tgatggcgaa gttcacgcgg gggtcgtcga gcatccactt
     2461 tgcgtagtcc ggcttcgcca cgaccggatc ggcggcgaag agctgcgagt agaagcgggt
     2521 cgaagtttcg agatcgtcta cggcgacgtg tacatgcagg cgtttcattt gcgtttactc
     2581 ctggaggtgc ttgaccatga atgccgcgct tgcgggacaa aggctcgcaa actcggcgga
     2641 tgaacgaatg aacggtggcg cgtcgctgcg gttagcgcct gcgtaaccgc gtgcggcaaa
     2701 gaagggctcg gcggtcgtgg tcaacagata gatccggtgc gctccgcgtg cccgggcctg
     2761 ctcttcggca tgctccagca ggcgcgagcc aaggccgccg gaccgagctt ctgctttgac
     2821 cacgagcgac cgcagtagcg catgcgggcg cgaaagctcg aggccgacga tgccgagcgg
     2881 atctgtccgc tcacccgcaa agtagaaact ttcgagaagc tcgtcggtta tatcggatgt
     2941 cggcagttga gcgcgactca ggagcgcgat gaccccctgc cgggaaggtt ggcctgaaat
     3001 gggttccatt gcagggttca tgcctgccga gtggacgtgc ggcgggattt gccacgcggc
     3061 gcggctgcag atgcgcatcc ggccccgccg cagcagtttt ccgtgagata tcccacgagc
     3121 tcgttcatca cgccgaagtc ggtcgcgtaa aagagctggc ggccaacccg ccgctgcgtg
     3181 agcagcccgg cgcgcgcgag gtgctgcaag tggaacgtga gcgaggacgg caccagtccg
     3241 atccgctccg caatctcacc ggccggcagt ccctccggtc ccttttccac gaggagacga
     3301 aagataccca atcgatgctc gtgtgccagg gctgcaagcg cagtgatgac gtcgggggta
     3361 ttcatgtacg ctacgataat acgagaatag tcgaaatgca agcaggcctg aatcccgtca
     3421 tggccagcaa ccgggccaag agcacacttc gcagagccaa tgaactgcct tgtacttcgc
     3481 tgtggagaac gaaggcgatg ctaatcgccg acgatgcatt tcacgacggc gtcaaaaaac
     3541 gccatgggat cgatgacttc cttgcccacc ggcttgggtt cggcggcatt ctgt
//
LOCUS       DASUOM010000070         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1719603,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000070 DASUOM010000000
VERSION     DASUOM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719603"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFY27_01500"
     CDS             complement(<1..316)
                     /locus_tag="VFY27_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5764213.1"
                     /translation="MKFSDSWLRAFVAPSATAEELAERLTMAGHEVDDIEIMGAKLDG
                     ILVTEVLAVKQHPDADRLSVCRVYDGDNEYEIVCGARNVAVGMKSILALPGATLPNGA
                     RLR"
     gene            complement(323..1306)
                     /gene="pheS"
                     /locus_tag="VFY27_01505"
     CDS             complement(323..1306)
                     /gene="pheS"
                     /locus_tag="VFY27_01505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5764214.1"
                     /translation="MQALHELVQRAKSEIDTAATLPALDAVRVAWLGKKGILTQRLKQ
                     LSALPQEERPAAGQQINKAKSEIQAGLEARRQHLEEEALTQKLSKDRVDVTLPGRGMS
                     LGGRHPVTRAMARIERIFRDAGFGVRTGPEIEDDYHNFTALNIPENHPARALHDTFYF
                     PGGKLLRTHTSPVQIRSMVEEGAPIRIIAPGRVYRRDSDQTHTPMFHQVEGLVVDRGI
                     SFANLKAVLHQFVEIFFERDAKLRFRASYFPFTEPSAEVDVLWGDDKWLEILGCGMVH
                     PNVLRNAGVDPAEFTGYAFGMGVERLAMLRYDVRDLRTFFENDLRFLRQFH"
     gene            complement(1389..1751)
                     /gene="rplT"
                     /locus_tag="VFY27_01510"
     CDS             complement(1389..1751)
                     /gene="rplT"
                     /locus_tag="VFY27_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5764215.1"
                     /translation="MARVKRGVTAKARHKKVLDKAKGYYGARSKVYRVAKQAVIKAGQ
                     YAYRDRRQRKRQFRALWITRINAAARLHGISYSQLINGLHKSAIEIDRKVLADIAVHD
                     PEAFGAIAAQAKAGLGES"
     gene            complement(1767..1964)
                     /gene="rpmI"
                     /locus_tag="VFY27_01515"
     CDS             complement(1767..1964)
                     /gene="rpmI"
                     /locus_tag="VFY27_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5764216.1"
                     /translation="MSKMKTNRGAAKRFRRTGKGGFKRAQSHLNHILTKKRSKRKRQL
                     GTTEQVADSDQKSVRRMLPYS"
     gene            complement(2081..>2439)
                     /gene="infC"
                     /locus_tag="VFY27_01520"
     CDS             complement(2081..>2439)
                     /gene="infC"
                     /locus_tag="VFY27_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5764217.1"
                     /translation="DPPVCRVMDFGKYLFELNKKAQTAKRKQKQVHVKEIKFRPGTDE
                     GDYKIKLRKLIDFLQSGDKTKVTLRFRGREMAHQELGAKLLARVRNDLDAIGTVEQMP
                     QLEGRQMVMVMAPKKK"
BASE COUNT          417 a          754 c          705 g          563 t
ORIGIN      
        1 gccgcagcct ggcaccgttc ggcaacgtcg cgcccggcag cgccagtatg ctcttcatcc
       61 ccacggccac gttccgggcg ccgcaaacga tctcgtactc gttatcgccg tcatagacgc
      121 ggcaaacgga aagcctgtcg gcgtcgggat gctgctttac tgcaagcact tcggtgacca
      181 ggatgccatc gagcttcgca cccatgatct cgatatcgtc cacttcatgg cccgccatcg
      241 tgagtctttc agcgagttcc tcggcggttg ctgatggagc aacgaaggcg cgcagccagc
      301 tgtctgaaaa tttcatcgcg attcagtgaa actggcgcag gaagcgaagg tcgttttcga
      361 agaacgtgcg caggtcacgc acgtcgtagc gcagcattgc caggcgctcg acgcccatgc
      421 cgaaggcata gccggtgaat tctgcagggt ccacgccggc gttccgcagc acattggggt
      481 gcaccatgcc gcaacccagg atctcgagcc acttatcgtc gccccacagc acgtcgacct
      541 cagccgaggg ttccgtgaac gggaaatacg aggcgcggaa tcgcaacttt gcgtcgcgct
      601 cgaaaaaaat ttcgacgaac tggtgcaaca ccgctttcag gttggcgaag ctgatgccgc
      661 ggtcgacaac cagaccttcg acctggtgaa acatcggcgt gtgggtttga tctgaatcac
      721 gacggtacac ccgccccggc gcaatgatgc ggataggagc tccctcttcg accatcgagc
      781 ggatctgcac cggcgaggtg tgagtacgca gcagctttcc gccgggaaag tagaaggtgt
      841 cgtgcagcgc tcgagccggg tgattttccg ggatattgag cgccgtgaag ttgtggtaat
      901 cgtcctcgat ctcgggaccg gtgcggacgc caaagccggc atcgcggaag attctttcga
      961 ttcgcgccat cgcgcgggtg acgggatgac gtccgccaag tgacatgccg cgaccgggaa
     1021 gggtcacatc cacccgatcc ttgctgagct tctgtgtcaa ggcctcctct tcaaggtgtt
     1081 gccgcctggc ctccagtcct gcctggattt ccgacttcgc cttgttgatc tgttggccgg
     1141 cggcgggccg ctcctcctgc ggcaatgcgc tcagttgctt gagcctctgg gtcaggatcc
     1201 cctttttacc cagccacgcc acccttacgg catcgagagc cggcagggtg gcagcggtat
     1261 caatttcgga ttttgcccgc tgaacgagct cgtgcagtgc ctgcatcgga gtccccaaca
     1321 aaaagcggcg tgccggtatg ctgcgcagcg ggactttccc tgctggcgct cgccgcttcc
     1381 gatccaggtc agctttcgcc gaggcctgct tttgcctgcg cggctatggc gccgaacgct
     1441 tcagggtcgt ggacggcgat gtccgccaac accttgcgat cgatctcgat ggccgacttg
     1501 tgcaggccat tgatcagctg gctgtacgaa atgccgtgca ggcgcgccgc agcattgatg
     1561 cgggtgatcc agagtgcccg gaactggcgc ttgcgctggc gccgatcccg gtaagcgtac
     1621 tgtccggcct tgatgacggc ctgtttcgca acccggtaga ctttgctgcg cgcgccgtaa
     1681 taacccttgg ctttatccag gactttcttg tggcgtgctt ttgcggttac gccgcgtttg
     1741 actcttgcca tttcgaatga ctcctcttaa ctgtaaggga gcatgcggcg cacactcttc
     1801 tgatcgctgt cggcaacctg ctccgtcgtg ccgagctggc gcttacgctt cgatcgcttc
     1861 ttcgtgagaa tgtggttcag atgggactgg gctcgcttga agccgccctt tcccgtccgg
     1921 cgaaagcgct ttgcagcgcc ccggttggtc ttcattttcg acatttctgt ctccgaattt
     1981 tatggcctgg cagcgtacgt tcacgggcgc cgaagccccc gtcgccgctg caggcgggta
     2041 attggtcgcg accgcacttc aggtgcagcc gtcccgttac tcactttttc tttggtgcca
     2101 tcaccatgac catctgccgc ccttccagtt gcggcatctg ctcgacggta ccaattgcat
     2161 cgaggtcatt cctgaccctt gccaggagct tggcgcccag ttcctgatgc gccatctcgc
     2221 ggcctctgaa tcgcagggtg accttggtct tgtcaccact ttgcaggaaa tcgatcagct
     2281 tgcgcagctt gatcttgtaa tcgccttcgt ccgtaccagg gcgaaacttg atttccttta
     2341 cgtgtacctg tttttgtttg cgcttcgctg tctgtgcctt cttgttcagc tcaaacaggt
     2401 actttccaaa atccatcacc cggcataccg gcgggtctg
//
LOCUS       DASUOM010000071         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_440115,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000071 DASUOM010000000
VERSION     DASUOM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440115"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1045
                     /locus_tag="VFY27_01525"
     CDS             74..1045
                     /locus_tag="VFY27_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5764218.1"
                     /translation="MTERILLVIAAALFVPALLVSAAYLADMRREYARVRANGTVIPS
                     PCGDIEFTEGGAGPDALVIHGSGGGYDQGELIADAVLGANFRSITPSRFGYLRSTFHE
                     GATWDDQAHVYRYLLDHLGVERVAVLAMSHGGPSALLFAALYPERVSSLTLVSCGVAT
                     SLDPGQAQADRKGKVLTSAFKFDFPYWLVSRLGKSQFMELMGATDTVIARLTPAQRTS
                     VGNIIDFMNPASLRSAGVAFDNTGTLPGRRVAEIKAPTLIFHAADDTLQLYHNAEFAV
                     STIPDARLVSFERGGHLAVIVEQSRIRTAAQAHIRLNMRERQEEQHQ"
     gene            complement(1089..1739)
                     /locus_tag="VFY27_01530"
     CDS             complement(1089..1739)
                     /locus_tag="VFY27_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX5764219.1"
                     /translation="MSDLLPTSPLLSAFLLTSFVLAVTPGPGVVYIVTRTVTEGRASG
                     FASAAGVALGNFGNALGASLGLAAIFSASSLAFEVVRYAGALYLLFLGWKALRTKGTR
                     AVPASNSTRKPGNRALRDGFFIALFNPKTALFFAAFLPQFVVPAASHPLQPIILGTLF
                     VITAAVTDALYATFASLAVPWFRRIRFAASAARYATGGAYLGLGLFAAFSGTRARQ"
     gene            complement(1766..2122)
                     /locus_tag="VFY27_01535"
     CDS             complement(1766..2122)
                     /locus_tag="VFY27_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5764220.1"
                     /translation="MALEQYEGGCQCGAVRFDVGVDLDNTITCNCSRCQRMGFVLAFS
                     PRDDFHLKSGEQALTEYLFNKKAIRHLFCSTCGVESFAYGKMPDGSPIVAINVNCLDD
                     VDPRSLKSQHVDGRSA"
     gene            complement(2480..2860)
                     /locus_tag="VFY27_01540"
     CDS             complement(2480..2860)
                     /locus_tag="VFY27_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5764221.1"
                     /translation="MHQSRLCAILIDCNTPDVDEAAAFWAAALGRPIDREHPGTRGNY
                     RMLETPADEPIVQIQRVEHESRAHIDIETDDISAEVARLEKLGAEIVSRLERWIVMQA
                     PTGQRFCVVRVQRPGFPRNATQWD"
     gene            complement(2930..3499)
                     /locus_tag="VFY27_01545"
     CDS             complement(2930..3499)
                     /locus_tag="VFY27_01545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008223076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX5764222.1"
                     /translation="MRTERQKMLAGELYNPQDEELVAARKRARDLCRSLNDTRESQQA
                     ERRVLLQDLFGSGGDTVWMQPPFWCDYGSHIHLGTRVFFNFNCVVLDVCEVRIGDYTL
                     FGPGVQILTPLHPLDPGLRREREFGKPVHIGSDVWVGGGAFISAGVTIGCRSVIGAGS
                     VVTRDIPAGVFAAGNPCRVIKDATQAPSA"
     gene            complement(3546..>4010)
                     /locus_tag="VFY27_01550"
     CDS             complement(3546..>4010)
                     /locus_tag="VFY27_01550"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX5764223.1"
                     /translation="GKPCFIFEGRNVAIIQPFKEHCSLMFFKGALMQDPHKLLRSQGE
                     NTQSAMRLEFTGKDQIKKTIVTAYIKEAIAVEKAGLKADFKAKRELELPEELTRIMNK
                     DSKLSKAFHALTPGRQRGYVLHFTGAKQSETRTARIEKSIPRILAGKGIHDR"
BASE COUNT          834 a         1211 c         1147 g          818 t
ORIGIN      
        1 gttgtaggcc gcaaaaactt gcaggaactc gcttcgggaa aagggtagga tcacgacggc
       61 aaaagctccc acgatgacag agcgtatcct acttgtgatc gctgcggcgc tgttcgtccc
      121 ggcgcttctg gtttccgccg cttatctcgc cgatatgcgg agggagtacg cacgagtccg
      181 cgcgaacggt accgtcattc cctctccctg cggcgacatc gaattcaccg aaggcggagc
      241 cgggcccgac gctctcgtga ttcatggaag cggcggcggc tacgaccagg gcgaactcat
      301 cgccgatgcg gttttgggcg caaacttccg gtccatcaca ccctcgcgct tcggatactt
      361 gcgttccacg ttccatgaag gtgcgacttg ggatgaccag gcgcatgtat accgctactt
      421 gctggatcac ctcggcgtgg aacgcgtcgc cgtcctcgcg atgtcccatg gcggtccctc
      481 tgccctgctc ttcgccgcgt tgtacccgga gcgtgtgtcg tcgctgaccc tcgtctcctg
      541 tggggtcgcc acctcactgg acccgggcca ggcacaggcc gaccgtaagg ggaaagtgct
      601 gacgtcagcc ttcaagttcg atttccccta ctggctggta agcaggctgg gaaagagcca
      661 gttcatggag ctgatgggcg ccaccgacac cgtcattgcg agacttaccc cggctcagcg
      721 gacatcggtt ggcaacatca tcgacttcat gaacccggca tcgttacgat ccgccggcgt
      781 tgcgttcgac aacacaggca ctttgccggg tcggcgcgtt gccgagatca aagcgcccac
      841 cctgatcttc catgctgccg atgataccct gcagctttat cacaacgctg aattcgcagt
      901 ctcaaccatt cctgatgcga ggctcgtttc ctttgaacgc ggcggccacc tggcagtgat
      961 cgtggagcag tcgaggattc gtacggccgc gcaggcacat attcgcctca atatgagaga
     1021 gcggcaggag gagcagcatc agtaaccggc ggccggtcaa ctttgcccgg gtgcactcgc
     1081 gaccgcagct actgcctggc acgcgtgccg gaaaaagccg cgaacagccc aagaccaaga
     1141 taagccccgc cagtcgcata tcttgctgcg gatgcggcga agcggatccg acgaaaccag
     1201 ggaacagcga ggctggcaaa ggtagcgtac aaagcatctg tcactgccgc ggtgattaca
     1261 aacagggttc cgaggatgat cggctgaagt gggtgcgatg ctgccggaac aacgaactgc
     1321 ggcaggaaag cggcaaagaa caaagccgtt ttcggattga aaagcgcgat gaaaaagccg
     1381 tcccggagcg cgcgattgcc cggctttcgg gttgagttgg aagccgggac agcccgcgta
     1441 cctttcgtgc gcaatgcctt ccagccaagg aacagaaggt acagtgcgcc tgcatagcga
     1501 acgacctcga aggccaggga tgacgcggag aaaatagccg caaggcctag cgaagctccg
     1561 agagcgtttc caaagttacc gagtgcgaca ccggccgccg acgcaaagcc ggacgcacgc
     1621 ccttcggtga cagtacgtgt gacgatatac actacgccag gtccaggagt gaccgccagc
     1681 acgaagctcg tgaggaggaa cgcggacagg agcggcgagg ttgggagcag gtcagacata
     1741 gatcaccgtc agatttccgc taagactatg cacttctacc gtcaacatgc tgacttttca
     1801 agctcctggg atccacgtca tcgagacaat tcacgttgat cgcaactatc ggtgatccat
     1861 ccggcatttt tccgtaggcg aagctctcga caccgcaggt gctgcagaag agatggcgaa
     1921 ttgccttctt gttgaaaagg tactcggtga gagcctgctc accagacttc aggtgaaaat
     1981 catcccgcgg tgaaaaagcc agtacaaagc ccatgcgctg gcagcgcgaa caattgcagg
     2041 tgatggtgtt gtccaggtcc acgccgacgt cgaaacgaac ggcaccacac tggcaaccgc
     2101 cttcatactg ctcaagtgcc atttctttaa cctccttccg ggatgtcagt ggtcttggcg
     2161 tcgcgagagc gccttgctct taaagacaga cgataccctc gacgtgacgt ggatgaaatc
     2221 agatcagccc cgatacgatc tccgggggaa tgtcgatcgg gtaagctccg ccaccttgtc
     2281 gcccgtgatt tcgaacagaa acacccggtt cgcctcgata tacctgccat cgaccgcgag
     2341 ttgatccgcc cagagcccga tccacgcctc gggatagccc agtgccgttt cgccgcgaac
     2401 gcgatggtgg taagttccgg tgcgtcggcc gtcgcagccc tgcgaatgtc tatgttcaag
     2461 gccgcacctt atccactggc tagtcccatt gagttgcatt cctggggaac cccggccgct
     2521 gcacccgcac gacacagaag cgctggcccg tcggagcctg cattacgatc cagcgctcca
     2581 ggcgactgac tatttccgct ccaagctttt caaggcgagc tacttcagca gaaatgtcgt
     2641 cggtctcgat gtcgatatgg gcacggctct cgtgctcgac tcgctggatt tgtacgatgg
     2701 gctcgtcggc tggcgtctcc agcatacgat agttgccgcg cgtgcccgga tgttcgcgat
     2761 cgataggacg gccaagggcc gccgcccaga aggccgccgc ttcatcgaca tccggagtgt
     2821 tacagtcaat caatattgcg cacagcctgg actggtgcat cgtattacct ccgccttacc
     2881 gtgacgtttt acctgtgccg agcgcttcaa atgaaatcac gcccgatcgt caggcgctcg
     2941 gtgcctgcgt agcatctttg atcacgcgac acggattgcc ggcggcgaaa actcccgctg
     3001 gaatgtcacg tgtgaccacg ctcccggcac cgataacgga tcggcaaccg attgtaacgc
     3061 cggccgatat aaatgcgccg ccaccgaccc acacgtcgga gccgatgtgc acgggcttgc
     3121 cgaattcccg ctctcttcgc agccccggat caaggggatg caggggcgtg agtatttgca
     3181 cgcctgggcc aaacaaagtg tagtccccga tgcgaacttc gcagacatca agcaccacgc
     3241 aattgaagtt gaagaacacc cgggtgccaa ggtggatatg cgacccgtaa tcgcaccaga
     3301 acggcggctg catccagaca gtatcgccgc ctgagccgaa gagatcctgc aacagtaccc
     3361 gtcgttctgc ttgctgcgat tcccgcgtgt cgttcagtga acggcataaa tcgcgtgccc
     3421 gcttacgtgc ggcgaccagt tcctcgtcct gcgggttgta cagctccccg gcaagcattt
     3481 tctggcgctc agtgcgcatc tgtccgtgca gggacatctc aggcggccga cctcttggct
     3541 cgcggctaac gatcgtgaat gcccttcccg gcgagtattc tgggaatcga cttctcgatt
     3601 cgcgccgtac gagtttcgga ttgcttcgcc cctgtgaagt ggagaacgta gccgcgctgt
     3661 cgtccgggag tcagggcatg aaaagccttt gacagcttgc tgtccttgtt catgatccgg
     3721 gtaagctctt ccggaagctc cagctcgcgt ttcgccttga agtcggcctt cagcccggcc
     3781 ttctccacgg cgatggcctc ttttatatac gccgtcacga tggttttctt gatttgatcc
     3841 ttaccagtga attccaatcg catcgcggac tgtgtatttt cgccttggct gcgaagcagt
     3901 ttgtgcgggt cctgcatcag ggcgcccttg aagaacatta acgaacaatg ctctttaaat
     3961 ggttgaatga tggccacgtt cctcccctcg aatatgaagc acggcttgcc
//
LOCUS       DASUOM010000072         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_198769,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000072 DASUOM010000000
VERSION     DASUOM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198769"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..425
                     /locus_tag="VFY27_01555"
     CDS             24..425
                     /locus_tag="VFY27_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5764224.1"
                     /translation="MLWVLRDEVGLTGTKFGCGIGACGACTVHVDGRAQRSCMTAIGT
                     LANRSITTIEGLSADATHAVQRAWLEEDVPQCGYCQAGQIMAVTDLLRQVPDPTDEDI
                     DRLHTNICRCGTYYRMRKAIHRAAAILAEEA"
     gene            427..2646
                     /locus_tag="VFY27_01560"
     CDS             427..2646
                     /locus_tag="VFY27_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5764225.1"
                     /translation="MHAPILTRRHFLQVSLTASGALLLGLKLPLDVRAANASDLNAFV
                     RIEPGGATIIGVSQPDMGQGMYTTMSMLIAEEMDADWSRVTTRQLPLMLKRDDQGQMA
                     WKFVPQGAGGSTSVVDLYRPLREFGAEARQLLVRAAAAEWQVPAGEITTEPGMVIHRQ
                     SNRRAAYAELASRAAALEPGDRVPELKAPANFRLIGKQVVPKAVREVVSGRTRYGIDV
                     QLPGMRHAVIARCPYFDGTLKEFDDSEARKVKGVLDVVSIAGPPPGAPATILAPGVAV
                     VAESTWAAIKGRNALKIRWNEGPHHEETTAAFDARCQAELDTGEGEVVRDDGDFAAVL
                     DASSRSITQRYRLPYAHHATMEPQNCYADCRDDRCTVIGPMQMPSSASRLVAELTGLD
                     RMAIDVQMTRIGGGFGRRLTSDYAAEAVLVSKAAGVPVKVTWTREDDMAHDFLRPGGW
                     HELTAALGEDGMPKAWRHRVASPSKYYRREGEKLSESEIYIDDPPAGLVEHVRYEYLP
                     MQSGAWRGSWRAPAHTANAFTVQSFIDELAQATGQDPLEYRLRMLGERRELPYSNHGG
                     PTWNPGRLAEVLRVAAREAGWGREMPSGEGLGIAGHFTFGGYAAWVAHVKVDRDGKLT
                     VRRLTGAVDCGLAVNPNGIRAQMEGGACDALSTALGEELTIEGGRHRETNFHQYRLMR
                     IDQAPRSIDVHIVQGADEPAGLGEPPVPPLAPALTNAIFAATGRRIRHLPVGDQLRS"
     gene            2840..3724
                     /locus_tag="VFY27_01565"
     CDS             2840..3724
                     /locus_tag="VFY27_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5764226.1"
                     /translation="MSILPKLEEAGSIALESLKSFFSGEPFQLAAALSYYTMLSIAPL
                     LLVMIGVSGFLLGEEQVREALVMQVRDLVGDEGASLLQTVSQNVGTNDRNYVSMIVGF
                     VLMVLGATTVFAQLQTSLNRVWGVKAEPANAILGFLRSRLLSLTVVLGLAFLLLVSLI
                     FSAAISAFQTYLEGFLPGADMIWTIANHAISLVLVALLLAMLFRYVPDVEISWRDTVI
                     GALVTATLLTLGKYAIGVYLGRAGVGSPYGAAGSAVVFMVWVYYVSLILFLGAEITKT
                     IARHRGNPLRPSAHAKRI"
     gene            3862..4839
                     /locus_tag="VFY27_01570"
     CDS             3862..4839
                     /locus_tag="VFY27_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5764227.1"
                     /translation="MAYGLLAWLFGLAGLFHDNLIFNAGATLLLAHGMVIAAYMVHEC
                     GHNTVFRRVRHNEQLGSALTWICGASYGTFADIRYKHMRHHVDNDDVVWFDYEKFFLE
                     HPLALRVTRVLEWFYIPAHDLLMHGIMVFTSFVIPQRRNQRRRNVAVVLVRGGVFVAL
                     LFFYPKVAILYCIAYIVMVQVLRFTDSLQHDYPYRLNLYTDERSPHRGDKQWEQEHTF
                     SVPLSLRHDWLNWLTLNFGYHNAHHSRPTTPWYRLPALHRELYGNDESAVIPLSRQLV
                     IFHRGRVRRIIKWDDDAVDARTPTGREFLEAARQAQVDGGNAANFLTSF"
     gene            complement(4932..>5236)
                     /locus_tag="VFY27_01575"
     CDS             complement(4932..>5236)
                     /locus_tag="VFY27_01575"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5764228.1"
                     /translation="LAASGWHTAAVTMRMLVDGGLKVAGGLVGAGMEELRWPKPVRPG
                     DELHVEAEVLGKRESRSRPRQGIVRVRNTTFNQDDEVVQVSVSAMIVPRRNPGSKQ"
BASE COUNT          962 a         1689 c         1632 g          953 t
ORIGIN      
        1 acgacggcga tcccgaacag ccgctgcttt gggtgctgcg cgatgaggtg ggtcttaccg
       61 gaaccaagtt cggttgcggc atcggcgcct gcggcgcctg cacggtgcac gtcgacggcc
      121 gcgcgcaacg ctcctgcatg acagcgatcg gcacgttggc gaatcggagc atcacgacca
      181 tcgaaggcct ttccgcggat gctactcacg ccgtacagcg ggcatggctc gaggaagatg
      241 tgccgcagtg cggatactgc caggcagggc agatcatggc ggtaaccgat ctcctgcggc
      301 aggtaccgga tccaacggac gaggatatcg atcgactgca taccaacatt tgccgttgcg
      361 gcacttacta ccgcatgcgg aaggcgatcc atcgcgctgc cgcaatcctc gcggaggaag
      421 catgacatgc acgcgccgat cctgacgcga cggcacttcc tccaggtttc cctcacggcc
      481 agcggcgcac tgctgctcgg actcaagctt cctctggatg tccgcgcagc caatgcttcg
      541 gatctcaacg cgttcgtccg catcgagccg ggcggcgcga cgatcattgg cgtttcgcag
      601 cccgacatgg gacagggcat gtacaccacg atgagcatgc tgatcgccga ggaaatggac
      661 gccgactggt cgcgcgtcac cacccgtcaa cttccgctga tgctgaagcg agacgaccag
      721 ggccagatgg cgtggaagtt cgtaccgcaa ggcgccggtg gcagcaccag cgtcgtagac
      781 ctttaccgcc cgttgcgcga gttcggcgca gaggcgcgcc agctcctggt gagggccgcg
      841 gcagccgaat ggcaggtgcc ggccggtgag attacgactg agccgggtat ggtcattcac
      901 aggcagtcga atcgccgcgc cgcgtacgcg gaactggcct cgcgcgcagc ggcgctcgaa
      961 cccggtgatc gcgtgcccga actgaaagca ccggcgaact tccgtcttat tggcaagcaa
     1021 gtcgttccga aggcagtccg cgaagtcgtt agcggccgta cgaggtacgg catagacgtg
     1081 cagttgcccg gcatgcggca cgccgtaatt gcacgttgcc cgtacttcga cggcacgctc
     1141 aaggagttcg acgacagcga agcgcgtaag gtaaagggcg tgctggatgt cgtttcgatt
     1201 gcaggccccc caccgggcgc gcccgcgacc atcctggcac ccggtgttgc ggtggttgcc
     1261 gaatccacct gggcggcgat caagggccgt aacgcgctga agattcgctg gaatgaggga
     1321 ccgcatcacg aggagaccac agcagccttc gatgcccggt gccaggccga gctcgatacc
     1381 ggcgagggag aggtcgtgcg cgacgacggc gacttcgcgg ccgtgctcga tgcgagctcc
     1441 cgatcaatca cgcagcgtta ccgcttgccg tacgcgcacc acgcaaccat ggaaccgcaa
     1501 aactgctacg ccgactgccg cgacgaccgc tgcaccgtga tcgggcccat gcagatgccc
     1561 tccagcgcgt cgcgcctggt ggcggagctt accgggcttg acaggatggc gatcgacgtg
     1621 cagatgaccc ggatcggcgg cgggttcggc cggcgcctca cctccgacta tgccgccgag
     1681 gccgtgctgg tttcgaaagc ggccggggtg ccggtcaagg tcacctggac ccgtgaagac
     1741 gatatggcgc acgacttcct ccggccgggc ggctggcacg aactgacggc ggccctcggc
     1801 gaggatggaa tgccaaaagc gtggcggcac cgcgtcgcca gcccctcgaa atactaccgg
     1861 cgcgaaggcg aaaagctttc ggagtcggag atatatatcg acgatccccc ggcagggctc
     1921 gtcgagcatg tgcgctatga atatctaccg atgcagtcgg gagcctggag aggttcatgg
     1981 cgcgcgcccg cgcatactgc gaacgccttc accgtgcaaa gctttatcga cgaactcgca
     2041 caggcaaccg ggcaggatcc gctggagtat cgcctgagga tgctcggcga acggcgcgag
     2101 ctgccctact ccaaccatgg cgggcccacg tggaacccgg gccgcctggc ggaagtcctg
     2161 cgggttgccg cgcgcgaggc cggttgggga cgggagatgc cgtcgggcga gggcctgggc
     2221 atcgccgggc atttcacctt cggcggttac gcggcatggg ttgcccacgt gaaagtcgac
     2281 agggacggaa agttaaccgt ccgccgcctg acgggcgcag tcgactgcgg actagcagtc
     2341 aacccgaatg gtatccgcgc gcaaatggaa ggcggagcct gcgacgcact cagcaccgca
     2401 cttggtgagg aactcaccat cgagggcggc cgccaccgcg aaacgaactt ccaccagtac
     2461 cgcctgatgc gcatcgacca ggctccgcga agcatcgacg tacatatcgt gcaaggggcc
     2521 gacgagccgg ccgggctggg agaaccgcca gtgccgcctc ttgcacccgc tctcaccaac
     2581 gccatcttcg ctgcaaccgg cagacgcatc cgccatcttc cggtaggaga ccagctccgg
     2641 tcctgatccc cgggcctccg tcatagcggc attatttgca acccgggcgg caaaaattac
     2701 accgggagtg gcgccagccg gccagccctc tgcgcggcaa tggggaggcg tggcagcgtc
     2761 ttggcacatc gcttgcagtt tgccgcgagt atgtcgtccg ctctgcgcga gcaacttctg
     2821 ccgcactgga acgccgatct tgtccatatt gcccaaactc gaggaagccg gctcgatcgc
     2881 gctcgaatcc ctgaagagct ttttctcggg agaaccgttc cagttggcgg cggcgctctc
     2941 ctattacacg atgctgtcga tcgcgccgct tctgctcgta atgatcggcg tgtccggatt
     3001 cctgcttggc gaggagcagg tgcgggaagc actggtgatg caggtgaggg acctggtagg
     3061 cgatgaaggc gcctcgctgc tgcagaccgt ttcgcaaaac gtcggcacga atgaccgtaa
     3121 ctatgtctcg atgatcgtgg gtttcgtgct catggttttg ggtgccacca cggtatttgc
     3181 ccagctgcag acgtcgctga accgcgtctg gggcgtgaag gcagagcccg ccaacgccat
     3241 actcgggttt ctccgctcgc gcttgttgtc gctcacggtc gtgctcgggt tggcgttcct
     3301 gttgctggtg tcgctcatct tcagcgccgc gataagtgcg ttccagacgt acctggaagg
     3361 tttcctgccg ggcgcggaca tgatctggac catcgccaac catgccattt cccttgttct
     3421 cgtggcactg ctgctggcga tgctgttccg ctatgtgccg gacgtggaaa tatcctggcg
     3481 cgacacggtc atcggtgccc tggtcaccgc cacgctgctg acccttggaa agtatgcgat
     3541 aggcgtctac ctgggacgcg ccggcgtagg ctccccctat ggcgccgccg ggtcggccgt
     3601 cgtcttcatg gtctgggtct actacgtctc cctgatcctg tttctcggtg ccgaaatcac
     3661 caaaaccatc gcccggcacc gcggtaaccc gctcaggccg agcgcgcacg caaaacggat
     3721 ttgataacat ttgcagctgt ccaggcagca tcgagctctc agagaagagg gaacgcctga
     3781 ccaacggatc cagagacggc agtggcagca ggctcaagcg ctttcaggga accccagggt
     3841 gccgtctacc acggcgctgc agtggcatac ggtctgctcg cctggttgtt tggcctcgcc
     3901 ggactgttcc acgacaacct gattttcaat gccggggcaa ccctcttgct cgcgcacggc
     3961 atggtcatcg ccgcttacat ggtgcacgaa tgcgggcaca acacggtgtt ccggcgcgtg
     4021 cggcacaacg aacagctggg cagcgcgctc acctggatct gcggcgcttc ctacggcacg
     4081 ttcgcagaca tccgctacaa gcatatgcgc catcatgtcg ataacgacga cgtcgtatgg
     4141 ttcgactacg aaaagttttt cctcgagcat ccacttgccc tccgcgtgac acgcgtgctg
     4201 gagtggttct acatcccggc gcacgacctc ctgatgcacg gcatcatggt gttcacttct
     4261 ttcgtcattc cgcagcgccg caaccagagg cgcaggaacg tagcggtggt cctggtgcgc
     4321 ggcggcgtgt tcgtcgcgct gctgttcttc tatccgaagg tcgcgatcct gtattgcatc
     4381 gcgtacatcg tcatggtgca ggtactgcgg ttcacggaca gcctgcagca cgactaccca
     4441 tatcgcctga acctgtatac cgacgagcgc tcgccccatc gcggcgacaa gcaatgggaa
     4501 caggaacaca ccttcagcgt ccctttgtcg ctgcgccacg actggctaaa ctggctgacg
     4561 ctcaacttcg gctatcacaa cgctcatcat tcccggccga cgacaccgtg gtaccggttg
     4621 ccggcgctac atcgcgagct ctacggtaac gacgaaagtg cggtaatccc gctatcccgg
     4681 caactcgtca ttttccatcg cggcagggtc cggcggatca tcaagtggga cgacgacgcc
     4741 gtcgatgcaa ggaccccgac tggccgcgag tttctggaag ccgcgaggca ggcgcaggtc
     4801 gacgggggca acgccgcaaa cttcctcacc tcgttctgaa ccgctcgaac ggcacgctgc
     4861 agtagctcgt ccacggacga cattccacat gaccttgtgt taacgcggcg cccgaagatg
     4921 ggcgtgcatg actactgttt ggagccggga ttgcggcgcg gcacgatcat ggcgctcact
     4981 gacacctgca cgacctcgtc atcctggttg aacgtcgtat tgcgaacccg aacgattccc
     5041 tgccgggggc gcgaccttga ttcccgcttg cccagcactt cggcctcgac atgcagctcg
     5101 tcgcccggcc gtaccggctt cggccagcgc agctcttcca ttcccgctcc gaccagtcca
     5161 cccgcgactt tcagaccgcc atccacgagc atgcgcatgg tgacggctgc cgtgtgccat
     5221 ccactggcgg ccaagc
//
LOCUS       DASUOM010000073         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1353786,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000073 DASUOM010000000
VERSION     DASUOM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353786"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1457)
                     /locus_tag="VFY27_01580"
     CDS             complement(297..1457)
                     /locus_tag="VFY27_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5764229.1"
                     /translation="MIDTANRPLTALADEERMFRDAVAEFAESEVRPRVREMDSAKRI
                     DPQLITSCFELGLMGIEVPGEFGGAGGSLFMVTIAVEELSRVDASAAILVDVQNTLVN
                     HPMVTYGSPEQQSRFLPRLTSDMVGAYALSEAGSGSDAFGLETRAERAEGGGWRLNGR
                     KLWITNGAEAGFFIIFANADPQAGYRGITAFLVERDYPGFAVGKKEDKLGIRASSTTE
                     LLLDQCVVPDENVLGPVGQGYKIAIETLNLGRIGIGAQMIGIARGALEATLTYVQDRK
                     QFGRAIAEFQAVQFQLAQAATELEAARLTVYNAARLKDAGLPFAQEAAMAKLFSSQVC
                     ERVTSLCVELFGGSGYTTDHPVEKFYRDAKIGTIYEGTSNMQLQTIAKGLLR"
     gene            complement(1510..>2403)
                     /locus_tag="VFY27_01585"
     CDS             complement(1510..>2403)
                     /locus_tag="VFY27_01585"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5764230.1"
                     /translation="TSVEIVPPRGVDASRMLEGVRALKAAGIDAVNVPDGPRAQSRMG
                     ALMTSLLIEQQVGIESVTHYCCRDRNLLGMLSDLLGASAIGLRNMLLITGDPPKMGPY
                     PDATAVFDIDSIGLTNLVDRLNHGLDPGGNAIGTPTRFVIGVGVNPAAIDPETEIRRF
                     EWKVEAGAEFAITQPVFDVAQLESFLARVSHVRIPVMAGIWPLVSARNAEFLANEVPG
                     VVVPQEIIDRMRAANAISKDRAVQEGIAIAREMLERVRESVQGVQVSAPFGKVELALE
                     VIGGGRTLEAERERGNGKGET"
BASE COUNT          441 a          805 c          701 g          456 t
ORIGIN      
        1 tagcgagtgc acgaacgtcc aggggtcgtt cgcgcgggca gcagtatatg ccgcgctcgc
       61 cgcgttgaaa agatcctttc caccctcctg aatcactttg ttctgtgaag tcgttattgg
      121 tgatttacat caccagtgtg ccgcggttga cgtcaccaaa caggcggata attcgaggtt
      181 gagagcagaa aagcttggcg gttgaggacc ttggaagaaa gcgcggcagg cgttcggttc
      241 gcctggctcg gccggcccag gccgctcgag gggcggcgcg gaccgccatc gttatgctag
      301 cgcagcaggc ccttggcgat cgtctggagc tgcatgttcg aggtgccttc gtagatcgta
      361 ccgatcttcg cgtcccggta gaacttctcg acggggtgat cggtcgtgta gccggatccg
      421 ccgaacaatt cgacgcataa cgatgtaacg cgctcacaca cctgggatga gaacagcttg
      481 gccatcgccg cctcctgggc gaacggcagg cccgcatcct tcaggcgcgc ggcattgtaa
      541 accgtcagtc gtgccgcctc cagttcggtc gccgcctggg cgagctggaa ctgtaccgcc
      601 tgaaactcgg ctattgcccg gccgaactgc ttccggtcct gcacgtaggt gagcgttgcc
      661 tccagtgccc cccgggcgat gccgatcatc tgcgcaccga tgccgatcct gccgaggttc
      721 agcgtttcga tggcgatctt gtagccctgg ccgaccgggc caagcacgtt ctcgtcaggc
      781 accacgcact ggtcgagcag cagttccgtc gtgctcgaag cgcgaatccc gagcttgtcc
      841 tccttcttac cgaccgcaaa acccggatag tcgcgctcta cgagaaacgc cgtgattccc
      901 cggtagccgg cctgcggatc ggcattggcg aaaatgatga aaaatccggc ctcggcaccg
      961 ttcgtgatcc acagcttgcg cccgttgagc cgccagcccc ctccctccgc gcgctccgcc
     1021 cgggtctcca ggccgaacgc atccgatccg gacccggctt ccgacagcgc atacgctcct
     1081 accatgtcac tcgtcaggcg gggcaggaac cgcgactgct gttcgggtga gccatacgtc
     1141 accatcggat gatttacgag cgtgttctgc acatctacca gaatggccgc cgacgcatcg
     1201 acgcggctca gctcctccac cgcgatcgtc accatgaaca gcgagccgcc cgcaccgccg
     1261 aactctccgg gcacctcgat gcccatcaac cccagctcga agcaggaagt gatcagctga
     1321 ggatcgatcc gcttggcgct gtccatttcg cgcactcgcg ggcgtacctc ggactcggcg
     1381 aattccgcaa cggcatcgcg gaacatgcgc tcttcatcgg cgagcgcggt cagggggcga
     1441 ttcgcggtat cgatcatctc ggaattctag gacgggaaat gggaaacggg aaaggggaaa
     1501 tgggaggaac tacgtttccc ctttcccgtt tcccctttcc cgctctgctt ccaacgtccg
     1561 tccacccccg atcacctcca gcgccagttc caccttgccg aatggtgcgc tcacctgcac
     1621 accctgcacg gattcacgta cgcgctcgag catttcccgg gcgatggcta tcccctcctg
     1681 taccgcgcga tccttcgaga tggcgttggc cgcgcgcata cggtcgatga tctcctgcgg
     1741 cacgacgacg ccaggcacct cattcgccag aaactcggcg ttgcgtgccg agacgagcgg
     1801 ccagatgccg gccatgaccg gaattcgaac gtgcgacacc cgcgcgagaa aactctcgag
     1861 ttgcgccaca tcgaacaccg gctgtgtgat ggcgaattcc gcgcccgcct ccactttcca
     1921 ttcgaaccgg cggatctccg tttcgggatc gattgccgcg gggttgaccc ccacgcctat
     1981 cacgaacctc gtcggcgtgc caatggcgtt gccgcccgga tcgagcccgt gattgagccg
     2041 gtcgaccagg ttggtgaggc caatggaatc gatgtcgaac accgccgttg catccggata
     2101 aggacccatc ttgggcggat cgccggtgat cagcagcatg ttccgcaggc cgatggcgct
     2161 cgcacctaac aaatcggaca gcataccaag caggtttcgg tcgcggcagc aataatgagt
     2221 caccgactcg atcccgacct gctgctcaat gaggaggctc gtcattagcg cccccatccg
     2281 gctctgcgcg cgcggaccgt ctggcacgtt gaccgcgtcg atcccggctg ccttgagcgc
     2341 ccgcacccct tcgagcatgc gcgaagcatc gactccgcga ggcggaacga tctccactga
     2401 agt
//
LOCUS       DASUOM010000074         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1002913,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000074 DASUOM010000000
VERSION     DASUOM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002913"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFY27_01590"
     CDS             complement(<1..265)
                     /locus_tag="VFY27_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764231.1"
                     /translation="MLSFQRAGRKLRFRYLLLNRLLSFKHDKGSGSLGSLSRPDCRAI
                     PEGGLFLKSGGVAMKYLVSTAGALFTALAFNLSTVPVQTAAAQE"
     gene            complement(392..2056)
                     /locus_tag="VFY27_01595"
     CDS             complement(392..2056)
                     /locus_tag="VFY27_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX5764232.1"
                     /translation="MVINTSTSGMPGRRTAFSAVFIALVLCVSVDSKAAENYQDLVEL
                     FDDWRSFERPPLRDGAPDYTATRFKSAHKELKQYQARLNAIDISEWPIEQQVDWHVVR
                     AEMNGFDFNHRVLQPWVRDPAYYQSVWMNRSDVPAHEGPSHHALLEFWTYSLPLSAGE
                     RQRLIGDLNVIPPLLAQARGNLTGNARDLWNAGIKNIRDQVDNLEAIGTAVGASSNAG
                     LAAALTAAIDASSSFVTWLEEQAPSKTGPSGVGKENYTWYQRNVHYVPMTWEEEVMLL
                     KRELDRAWSSLELEEHRNRHVPPLTAVDSPEEYAERADAAASRFMAFLEDREIMTVKP
                     YMEPALREHLGQYVPPEQRNFFAIAMHYDPTTLYTHFYHWFDLAQMDLEPHASPIRRG
                     PLLYNIFDSRNEGTATGVEEMFMHAGLYDDNPHVREIVWIMLAQRAARGLGSLYAHAN
                     IMTMGEAGEFHAEMTPRNWMSKELDLLYFEQHLYMRQPGYGTSYVTGKYLLERLLADR
                     SKQTNGNISLRQFFDELNAAGNIPIALVRWQLTGDKSEIHALTGGH"
     gene            complement(2267..>2484)
                     /locus_tag="VFY27_01600"
     CDS             complement(2267..>2484)
                     /locus_tag="VFY27_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764233.1"
                     /translation="HWSPTYGGGDAEFDYKVVFAYPTFADIGADFERYANGGGRERSE
                     ETFGEIDECDDARVYATTSRRAAQLRD"
BASE COUNT          494 a          744 c          697 g          549 t
ORIGIN      
        1 cttcctgtgc cgcggcggtc tgcaccggaa cggtcgacag gttgaaagcc agagcggtaa
       61 acagcgcccc cgccgttgag acgagatatt tcattgccac tcccccagat tttaaaaaca
      121 gaccgccttc aggaatcgct cggcagtccg gccgtgatag agaacccaaa gaaccacttc
      181 ctttgtcgtg cttaaacgac aaaagccggt ttaacaataa ataccggaac cgtagcttgc
      241 gcccggcacg ctgaaatgac aacacattcg ttgatcaggc agaccaatag cgcacctgct
      301 gcttgttgcc ctatggcaac aaatgattac agccacgatc gacaagacgt ggggcttacg
      361 tctgcgcttt gcttccggcc agccctggct ttcagtgacc gcctgtcaat gcatgaattt
      421 cacttttgtc cccggtcaat tgccaacgca ccagggctat cgggatgttt ccggctgcgt
      481 tgagttcgtc aaagaactgc cgcagcgaga tgttgccgtt ggtttgcttg ctgcgatcgg
      541 cgagcaagcg ctcgagcaag tatttgcccg tgacgtaact tgtgccatag cccggctggc
      601 gcatgtagag atgctgctcg aagtagagca gatcgagttc cttgctcatc cagttacgcg
      661 gtgtcatctc tgcgtgaaat tcgcccgctt cacccatcgt catgatattg gcgtgagcat
      721 acagcgagcc gagacctcgt gcggcgcgct gcgccagcat gatccagacg atttcgcgaa
      781 cgtgcggatt gtcatcgtag agcccggcgt gcatgaacat ttcctcgaca ccggttgccg
      841 taccttcatt cctgctatcg aaaatgttgt acagcaaggg accacgacgt atcgggctcg
      901 catgcggttc caggtccatt tgcgcgaggt cgaaccagtg atagaagtgc gtgtaaagcg
      961 tggtcgggtc atagtgcatc gcaatggcga agaagtttcg ctgctccggc ggcacatact
     1021 ggccaaggtg ttcacgcaac gccggttcca tgtacggctt gaccgtcatt atttccctgt
     1081 cctcgagaaa tgccatgaat cggcttgcgg ctgcatccgc gcgctccgca tactcttccg
     1141 ggctatcgac agcggtcagc ggaggaacgt gtctattgcg gtgttcctcg agttccaggg
     1201 acgaccatgc gcggtccaac tctcgcttga gcaacatgac ttcctcttcc caggtcatcg
     1261 gcacgtagtg cacgttgcgc tgataccagg tgtagttctc cttgccgacg ccggatggcc
     1321 cggttttcga cggcgcctgt tcctcaagcc acgtaacgaa cgatgacgag gcatcgatgg
     1381 ccgcggtcag cgccgccgcg aggccggcgt tgctgctcgc accgacggct gtcccgatgg
     1441 cttcgagatt gtcgacctgg tcgcgaatgt tcttgatccc tgcgttccac aggtcacgcg
     1501 cgttgcccgt caggttgccg cgcgcctggg cgagcaatgg cgggataacg ttcaggtcac
     1561 cgatgagccg ttgccgttcc ccggcagaca ggggcaacga ataggtccag aactcgagca
     1621 gggcatgatg cgacggtccc tcgtgcgccg gtacgtcgct gcgattcatc cacacggact
     1681 ggtaataagc cggatcgcgc acccacggtt gcagcacgcg gtgattgaaa tcgaaaccgt
     1741 tcatttcagc gcgcaccacg tgccagtcaa cctgctgctc gattggccat tcactgatat
     1801 cgatcgcgtt caatcgggcc tggtattgct tcagttcctt gtgcgccgac ttgaatcttg
     1861 tcgcggtgta gtcgggtgcg ccgtcccgca gcggcgggcg ctcgaaagag cgccagtcgt
     1921 cgaacagctc gaccagatcc tgatagttct ccgccgcctt gctatcgacc gacacgcata
     1981 agaccaaggc aatgaaaact gccgagaatg cagttcgcct gcccggcatt cccgacgtgc
     2041 tagtgttgat gaccattgaa ttctcccccg tgtcgaaatg cggaatcgct gtcgcaattg
     2101 aattgtagcc agattcgaag cagtgtagac aacctgaaac acgcattaga gtgcacggta
     2161 acgattcatt gaggtggccc tcttttcatt tgatcctcag gccatcgttg ggtaacgaat
     2221 cagggcggca gctggagctg ccgccctgcg tctccctgca gccgcgtcag tcgcgcagtt
     2281 gggccgcgcg ccggctcgtc gtcgcgtaaa cccgtgcatc gtcgcattcg tcgatttctc
     2341 cgaaagtctc ttcggagcgc tcacgaccgc ctccgttcgc gtatcgctcg aaatcggcgc
     2401 cgatgtccgc aaacgtcgga tatgcaaaga ccaccttgta gtcgaactcg gcgtcgccgc
     2461 ctccgtacgt cggactccag tgcc
//
LOCUS       DASUOM010000075         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_81246,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000075 DASUOM010000000
VERSION     DASUOM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81246"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VFY27_01605"
     CDS             <1..1629
                     /locus_tag="VFY27_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764234.1"
                     /translation="SGVPFIGAFCAAMAVVVKIQLGALKYLRTAVDKMANALSKCSGK
                     RGLWKMMKGLEQLASVVHKSFPAIGIAESISIAQANGASFGIVVNGKFTNVAGQLTLP
                     VKAMDFKEHCPFVESGGPGFELQSYQCSEGPLEVGRERIKWTLLVPFTTLVAQPIFSG
                     MVAKHNSQIGCKNDPSENSKIPVTLNSLEQCVKYDAKARWSHVWAETVQLSAAQATLT
                     MNDFIPWRPRKEYAGSKGADDAVDAGELGGQLGGLGIPTGGPVGEAAPEKPLGKGNEL
                     IDIGTERVVDPVFNQSEVACDGARYPFYQPPKAFPDYDWNASRPGLTAAFSNPQFCLN
                     CQPIKNWGKFTWVSGQHVVGGPETSGGYFIRVERREIPPKDEEKEEEPKFVYVVETVS
                     LISAGTTEMDQEEFDKYLEEQGQGGVNAEGSQSSSGCGNDRPKPYVLDKGTSPSQEKD
                     FQDRLRFIGIVWKDLEEDPPFWSGFYDEPPPTQYAYAQGQVYNYLSEDTFTQDWRVRL
                     EQASLLEGFLKSDAAKGQGVGKFVGSAIGKVNNH"
     gene            1648..2229
                     /locus_tag="VFY27_01610"
     CDS             1648..2229
                     /locus_tag="VFY27_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX5764235.1"
                     /translation="MFKPQRSFRGSAAIEVLLLIPFIVLIMMLLVNMGYNGFRHRTTQ
                     GALRLAAFDFVAGLSTMSREKSAQAAESSVNQVFFAGEDKAATVTFSGQKGVASQMEG
                     NSGFLADMSSRETAAISVKRTPPYADLFPNTDLRAGLIVASNTWTYCEMKDKDQGTLG
                     LSQIDLVGKYGLWLFGGCGGKTFDFSCDDRCPK"
     gene            2226..3077
                     /locus_tag="VFY27_01615"
     CDS             2226..3077
                     /locus_tag="VFY27_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764236.1"
                     /translation="MTGNAYSIVGKAIGTLVLALGLVVMVGRAPFLPEREAISGDTPP
                     GHSLQNVKVEPGIMQRSSSPLLSPPFPEKLAGIDAGLGLYKARFAATDSMPGFATEEH
                     EVEGSCTGAGRRYLEETCPGCELLPRKPGDASPPMLMWKEKGDGSESLRAVSTDCISI
                     GPTGLPVVGTLQLGDGVADLSAGRAAGVALDVVPLVPGASRLAAVEIGNWFATFDNVV
                     RPSSAVGDMARALASRGWREVSGTEGQSQFEGERVFTNARDEVCVVTLSRQGDEYQLL
                     TVVSLQT"
     gene            3088..3855
                     /gene="cpaB"
                     /locus_tag="VFY27_01620"
     CDS             3088..3855
                     /gene="cpaB"
                     /locus_tag="VFY27_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5764237.1"
                     /translation="MNAKWTLGLAIVAGLVAVFLVQRQLDNMRGETITVYKAASDRAA
                     GEILGGDVEAVTIAAGVFPDLFKEAPTAELVDLVQTTPLRENVRAGDILLFRHFDSAV
                     DQRLPASIAPGMKAIAIPVDEVSSVGFFVQPGDTVDVLATFLGSDDVSGPQVNADMFE
                     MSTRAVVQAVKVLAVGDKQRPTERQLMEPYSSVTLLVTMEEAAKLIFARDFFGANMTL
                     VMRGDGDELVDPAAPRIGVNTADFNLIGNRPDAPLTP"
     gene            3864..>4423
                     /locus_tag="VFY27_01625"
     CDS             3864..>4423
                     /locus_tag="VFY27_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5764238.1"
                     /translation="MAALVHIYDLRSSRIGEYRLDGTLSVGRDAASDIVLPSPYISPR
                     HAELKSLNGEVYIHAVGFNGVFVNDVDVPPGSSRLIHSGDIASVPGYVLYITGLEETI
                     RHDELELEQQFSALKAAIHSELLSSAGLRNVVSDTNDSKVVETLRSKLMEILDRPQVM
                     SDALVTYIVKRDIQQQVVNTVVRGGLG"
BASE COUNT          878 a         1287 c         1451 g          807 t
ORIGIN      
        1 tccggtgtgc ctttcatcgg cgccttctgc gccgcaatgg cggtcgtggt caagatccag
       61 ctgggggcgc tcaagtacct gcgcacggct gtggacaaga tggcgaacgc cctctccaag
      121 tgcagcggca agagagggct gtggaagatg atgaaggggc tggagcaact cgcgagcgtg
      181 gtgcacaaga gcttcccggc catcggaatc gccgaatcga tcagtattgc gcaggcaaac
      241 ggagcgtctt tcggcatcgt cgtaaacggc aagttcacca acgtcgcggg tcagctcaca
      301 ctcccggtga aggcgatgga cttcaaggag cattgcccgt tcgtcgagag cggggggccc
      361 ggtttcgagc tgcagagtta ccagtgcagt gaaggcccgc tcgaagtcgg gcgcgagcgc
      421 atcaagtgga cgttgctggt gccgttcacc acgctcgttg cccagccgat cttttcgggc
      481 atggtcgcga agcacaactc ccagatcggc tgcaagaacg atcccagcga gaacagcaag
      541 attccggtca cgctgaactc cctcgagcag tgcgtcaaat acgacgcaaa ggcccgctgg
      601 tcgcatgtat gggccgagac ggtgcagctt tccgccgcgc aagccacgtt gaccatgaac
      661 gatttcattc cctggaggcc gcggaaggaa tacgccggct cgaagggcgc ggacgatgcc
      721 gtggacgcgg gcgagctggg cgggcagctc ggcggccttg gtattcccac cgggggccct
      781 gtcggtgaag ccgccccgga gaagccgctg gggaaaggca acgagttgat cgacatcggc
      841 accgaacggg tggtcgatcc ggtctttaac cagtccgaag ttgcgtgcga cggtgcgcgc
      901 tatccctttt accagccgcc caaggcattc ccggactacg actggaacgc cagccggcct
      961 ggtctcactg cggcattctc aaacccgcaa ttttgcctga actgccaacc tatcaagaac
     1021 tggggcaagt tcacctgggt ttccggccag cacgtcgtag gcggaccgga gacctccggc
     1081 gggtacttca tcagggtcga gcggcgggaa attccgccga aggacgaaga gaaagaggaa
     1141 gagccgaagt tcgtatacgt cgtcgagacg gtatcgctga tcagcgccgg cacgaccgag
     1201 atggaccagg aggaattcga caagtacctg gaagagcagg gccagggtgg cgtcaatgcg
     1261 gaaggcagcc agagcagcag tggttgcggc aatgacaggc cgaagcctta cgtgctcgac
     1321 aagggcactt cgccgtcaca ggaaaaggac ttccaggacc ggctgcgctt catcggcatc
     1381 gtgtggaagg atttggaaga agatccgccg ttctggagcg ggttctacga cgagcccccg
     1441 ccgacccagt acgcgtatgc gcaggggcag gtctacaact atctgtccga ggataccttt
     1501 acccaggact ggcgcgtgcg gctggagcag gcgagcctgc tggagggctt tctcaaatcc
     1561 gacgccgcca aggggcaggg agtcggcaag ttcgtcggca gcgcgatcgg caaagtcaat
     1621 aatcactagg gaggttctgc acaagtgatg ttcaagccac agcgctcttt tcgcggctct
     1681 gccgcgatcg aggtcctgct gctgatcccg ttcatcgtgc tcatcatgat gttgctggtc
     1741 aacatgggct acaacggttt ccggcaccgg accacgcagg gcgctttgcg actcgccgca
     1801 ttcgatttcg tagccggcct gtccacgatg agccgtgaaa aatccgcgca ggcggccgag
     1861 tcgtcggtca accaggtgtt cttcgccggc gaggacaagg ccgcgacggt taccttctcc
     1921 ggccagaagg gcgtagccag ccagatggag ggaaactccg gcttcctcgc cgacatgtcg
     1981 tcccgcgaga ctgcggcaat atcagtcaag cgaaccccgc cttatgccga cctgtttccg
     2041 aacaccgacc tgcgggccgg gctcatcgtc gcgtcgaaca cgtggaccta ctgcgagatg
     2101 aaagacaagg accagggcac gctcggtttg agccagatcg atctcgtcgg caagtacggc
     2161 ctctggctgt tcggcggatg cggcggcaag acctttgatt tcagctgcga cgaccggtgc
     2221 ccgaaatgac cggcaacgca tacagcatcg tgggcaaggc gatcgggaca ttggtcctgg
     2281 ccctcgggct cgtcgtgatg gtgggcaggg cgccgttcct gccggagcgc gaagcgatca
     2341 gcggcgatac gccgcccggg cactcgctgc agaacgttaa ggtagagccg gggatcatgc
     2401 aacgcagctc gtcgccgcta ctgtcgccgc cttttccgga aaaactggcc ggtatcgacg
     2461 cggggttggg actctacaag gcccgcttcg ccgccaccga ctccatgccg ggattcgcca
     2521 ccgaggaaca cgaggtcgag ggctcctgca ccggtgcggg gcgccgctat ctcgaggaaa
     2581 cctgtcccgg ctgcgagctg cttccacgaa agccgggaga cgcttcgcct ccgatgctca
     2641 tgtggaagga aaaaggtgac ggcagcgaat cgctgcgcgc tgtatcgacg gattgcatca
     2701 gcatcggtcc gaccggttta ccggttgtgg gtacgctgca actcggtgac ggtgtcgccg
     2761 acctttcggc cgggagagcg gctggcgttg cgctggacgt cgtgccgctg gtaccgggcg
     2821 cgtcgcggct ggccgcggtg gagatcggta actggtttgc gacattcgac aacgtggtgc
     2881 ggccgagcag cgcggtcggc gatatggcca gggcattggc gagccgcgga tggcgcgaag
     2941 tttcgggaac ggaagggcaa tcgcaattcg aaggcgagcg ggttttcacc aacgctcgtg
     3001 atgaagtgtg tgtagtcacc ctgagcagac agggtgatga ataccagttg ctgacggtcg
     3061 tgagcctgca aacgtgaggg gaaaattgtg aacgcaaagt ggacattggg gctggcgatc
     3121 gtcgccggac tggttgccgt gttcctcgtg cagcgccagc tggacaacat gaggggagag
     3181 acgattaccg tttacaaggc ggcgtcagat cgcgcggccg gcgagatcct cggcggcgat
     3241 gtcgaagcag ttaccatcgc cgcgggcgtc tttcccgact tgttcaagga ggcgcccacc
     3301 gcggagctgg tcgacctggt gcagacgacg ccgttgcggg aaaacgtgcg cgctggcgac
     3361 atactcctct tccggcattt cgattctgca gtcgaccaac gcctgcctgc gagcattgca
     3421 ccgggcatga aagcgattgc catcccggtc gacgaagtca gttcggttgg attcttcgtc
     3481 cagccgggcg acacggtcga cgtactcgcg acttttctcg ggagcgacga tgtgagcggg
     3541 ccgcaggtga atgcagacat gttcgaaatg tctacgcgcg ccgtggtgca ggcggtcaag
     3601 gtgctggcgg tgggcgacaa gcagcgcccg acagaacgcc agttgatgga gccttactcc
     3661 tcggtgacgc tgctcgtaac catggaggag gctgctaagt tgatttttgc ccgcgatttt
     3721 ttcggagcga atatgacgct cgtgatgcgc ggcgacggtg acgaactggt ggatcccgcg
     3781 gcaccacgca tcggcgtgaa taccgcggat ttcaacctca tcggcaatcg gccggatgcg
     3841 ccgctgacgc cataggcaag gaaatggcag cactcgtcca tatctacgat ctccgctcgt
     3901 cccgcatcgg cgagtatcgg ctcgacggca cgttgtccgt cgggcgggat gcggccagcg
     3961 atatagtgct gccgagtccc tatatctcgc cgcggcatgc cgaattgaaa tcgctgaacg
     4021 gcgaggtcta catccatgct gtaggcttca acggcgtgtt cgtcaatgac gtcgacgtac
     4081 ctccgggcag cagccggctg attcactccg gtgacatcgc atcggtcccg ggttatgtcc
     4141 tgtacatcac gggactcgag gagacgatca ggcacgacga gctggaactc gagcagcagt
     4201 tctccgccct gaaagctgct atccactcgg aactgctctc gagtgccggg ctgcgcaacg
     4261 tcgtgtccga cacgaacgac agcaaggttg tcgaaaccct ccgcagcaag ctcatggaga
     4321 tactggatcg cccgcaggtc atgtcggacg cgctggtcac gtacatcgtc aagagggata
     4381 tccagcaaca ggtagtcaat accgtcgttc gaggcggtct cgg
//
LOCUS       DASUOM010000076         5230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1410178,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000076 DASUOM010000000
VERSION     DASUOM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410178"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFY27_01630"
     CDS             <1..620
                     /locus_tag="VFY27_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX5764239.1"
                     /translation="GNSAGMTDPLVSTQLYMCSLAAAMLAEHFPFGDELAPLAFRFNR
                     IMANRFYEILDFVNLHYCLTQRTDTDFWIEVQKRDRINDRLQAKLDHWRHKPPSRSDF
                     EDQFFPGQAERPLPSSGLHGDYRSPVDTGGLWGYEAYEVLLYGMDFLREECDEWFGKN
                     RPDPQVLASVVQRLNLARKKLPPHDVWLKQVFGMPDYPVSKQAVN"
     gene            620..2239
                     /locus_tag="VFY27_01635"
     CDS             620..2239
                     /locus_tag="VFY27_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX5764240.1"
                     /translation="MAAKRILIVGGGSSGWMSAAYLDAVLNAEGQEPVEIGLIESPDV
                     PRIGVGEATIPSINHILAAIGIDEIEFLRRVDGTYKQAIKYVNWLDGSGEGYYHAFGR
                     YRPSPVDTSTLDWLTSDRSIPFSETISAQPVICESCLAPKTIDQADIGARFTYAFHMN
                     ALKFADYLCEIATRRGVRHYLDHVSDVEMSENGDIAAVNTRSGNRIEADLFVDCTGFA
                     ALLIEKKLGVGWVDCSQWQLCNRALTIQVPYEHNYPGYVRPNTLATAVSAGWIWEIPL
                     QNRRAWGYVHGSDYIGEDDAEKELRQFIGSIGDNFPARLVPYKVGHRVKSWVNNCIAI
                     GLSAGFIEPLESTGLYLSDLAAVMLTEHFPHGGDMGRMAFRFNRIMANRFYEILDFIN
                     LHYCLTRRNDTAFWREVQRPERINDRLKAKLEFWRVKPPSLADFEDQWFPGQPDLPLP
                     DGGLPGDHRSAIDTAGIFTLSSYEAILYGMDFLREECDQWFGKNRPPSRVPPNIARRL
                     QMAANRLPSHEEWLTRVVGMPEYRQMGQASG"
     gene            complement(2224..2943)
                     /locus_tag="VFY27_01640"
     CDS             complement(2224..2943)
                     /locus_tag="VFY27_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX5764241.1"
                     /translation="MMRSGIAPVTVPNMNFPGNFFHIGDLDTADLKVLVKNLTEEQWN
                     SFSVRQKRYEVHQHTQTIGLVYDPDFRHSHPTRLPILEVFEPALRPILWMTADHFEET
                     QSAQRLIRENGSGYFIRASLVRLKANCSIAEHRDMNFSLTHSHRVHLPIITNDQVLFS
                     VGQETINMREGQLTEINNRRMHSVRNNGPDDRVHLLLDFVLPGEQCCCGARLHPQTRC
                     SPPACEDTVVGRVPCTCYPEA"
     gene            3323..>5230
                     /locus_tag="VFY27_01645"
     CDS             3323..>5230
                     /locus_tag="VFY27_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5764242.1"
                     /translation="MARRSNRPGYSSSVERVPMLTPLAAAVLTALYPLGPAFAQDEAV
                     PSDEPVEEVIVTGSRIRRDTFSSAAPMEVVLSEAAAPRGITDVASLLQTTTVAAGAPQ
                     VTAASSSVFVENGGLGASTLSIRGLGPNRTLILLNGRRAGPSGVQGGVSSFDLNAIPM
                     TAIERVEILKDGASSIYGSDAVAGVVNIITKKEDGAEFDAFLSAPQESGGEQMRLSGS
                     WGKSFDRGRMRVTADYNKVEILQRRDRDFFDCEEHYIFDPETGERADLIDPRTGSYLC
                     DDLPWGHVWVYDYAESYGDGTTNVGPPTYLLQYDFDGSLAANGLPPLAAPTSPYHMTT
                     PAGWYPVARGDALSESLIDSQHPLHGTQTLVPENEAMTVFLEGEYNLTDSVTAYAEVL
                     LNRRETVDVGYKQIWTYIYNYDSAGLVDDDWGTDPFSAGWTGAQWLSPLSITDHADQE
                     VTVDYGRFVAGLTGESLPVLEDWSWDLSFQYSKSDGEYLNEQVLTDALEGQYNRTGSC
                     VGTTTPISNRPCVDIQWLDPDFLAGDIPQEVKNYLFDVETGNTEYTQWSVEGFMTGDL
                     FELPAGSVAGAIGVHYREDEILDTPGPITLVRDANGQPALDEDGLTFSNAWQADAAGI
                     TAGEDSTYAIFG"
BASE COUNT         1047 a         1549 c         1579 g         1055 t
ORIGIN      
        1 tggggaattc ggccggtatg acagatccgc tggtatcgac acagctgtac atgtgcagcc
       61 tggcggccgc gatgctggcc gaacatttcc cgttcggtga cgaactggcc cccttggcgt
      121 ttcgctttaa tcgaattatg gccaatcggt tctacgaaat cctcgacttc gtgaacctgc
      181 attattgcct cacgcagcga accgataccg atttctggat cgaggtgcag aaacgggatc
      241 gtatcaacga caggctgcag gcaaagctcg accactggcg ccacaagccg ccttcacgga
      301 gcgacttcga ggatcagttc ttccccgggc aggccgagcg gccgctgcct tcgagcggat
      361 tgcacggtga ctaccgcagc ccggttgaca ctggtggtct atggggctac gaagcctacg
      421 aggttctgct gtacggcatg gacttcctgc gcgaagagtg cgacgaatgg ttcggcaaaa
      481 atcgacctga cccccaggtg ctggccagcg tcgttcagcg gctgaacctc gcgcggaaaa
      541 agttgccccc gcacgatgtg tggctgaaac aggtattcgg catgccggac tatccggtgt
      601 ccaagcaggc tgttaactga tggccgcgaa aagaattttg atcgtcggcg gcggttcttc
      661 gggctggatg agtgccgctt acctggatgc cgttttgaat gcggagggtc aggagccggt
      721 tgagatcggg ctgatcgagt cgccggacgt acccaggatc ggcgtcggtg aggcgacgat
      781 cccgagtatc aaccatatcc tggccgcgat tggcatcgac gaaatcgaat tcctccggcg
      841 ggtcgacggc acctacaagc aggccatcaa gtacgtcaac tggctcgacg gcagcggcga
      901 aggctactac cacgcatttg gacgttatcg cccatcgccg gtcgatacct cgacgctgga
      961 ctggctcacc agcgatcgct cgatcccgtt ctccgagacg atctcagcgc agcccgtaat
     1021 ctgtgagtcc tgcctcgcgc cgaaaacaat cgaccaggcc gacatcggtg cgcgtttcac
     1081 ctatgccttc catatgaacg ccctgaaatt cgccgattac ctctgcgaga ttgcgactcg
     1141 ccgcggggtg cggcactacc tggatcatgt gtccgacgtc gagatgtcgg agaatggcga
     1201 tattgccgcc gtcaacacca ggagcggtaa ccgcatcgag gcggacttgt tcgttgactg
     1261 taccggcttc gccgcactgc tgatagagaa aaagcttggc gtcggctggg tggactgctc
     1321 gcagtggcag ctctgcaatc gcgcactgac gatccaggtt ccgtacgaac acaactatcc
     1381 cggctatgtg cggccgaaca cgctggctac cgcggtctcg gcagggtgga tatgggagat
     1441 tccgttacag aaccgccgtg cctggggcta cgtgcatggc agcgactata tcggtgaaga
     1501 cgatgccgaa aaggagctgc ggcaattcat cgggtcgatc ggcgacaact ttccggcgcg
     1561 cctggtgcct tacaaagtcg ggcaccgggt caagtcgtgg gtcaacaact gcattgccat
     1621 cgggctgtcg gccggcttca tcgagccgct ggaatctacc ggactttacc tcagcgatct
     1681 cgcggcggta atgctgaccg agcatttccc gcacggcggc gacatgggca gaatggcgtt
     1741 ccgctttaat cgcatcatgg ccaaccgatt ctacgagatc ctggacttca tcaatctcca
     1801 ctactgcctg acccggcgta atgacactgc attctggcgc gaggtccagc gcccggagcg
     1861 gatcaatgac aggctgaaag ccaagctcga gttctggcgg gtcaagccgc cttcgctcgc
     1921 ggatttcgaa gaccagtggt ttccaggtca gccggatttg cccttgcccg acggaggact
     1981 acccggcgat caccgcagcg ccatcgatac cgcgggaatc tttaccttgt cgagttacga
     2041 ggcaatcctc tacggcatgg attttctccg cgaggagtgc gaccagtggt tcggcaagaa
     2101 caggccgcct tcgcgtgtac cgccaaacat tgccaggcgc ctccagatgg cggcaaacag
     2161 actgccttcc cacgaggaat ggctgacgcg cgtagttggc atgcccgaat atcgtcaaat
     2221 gggtcaggct tcgggatagc aggtacaggg aacacggcca accaccgtat cctcgcaggc
     2281 gggcggactg caccgggtct gagggtgcag cctggcacca cagcagcact gttcgcccgg
     2341 cagcacgaag tcgagtaaca ggtggacccg gtcgtccggg ccattgttgc gaaccgagtg
     2401 catccgccgg ttgttgattt cggtcagctg tccttcccgc atgttgatgg tctcctgacc
     2461 gacggaaaaa agcacctggt cgtttgtaat tatcggcagg tgcacgcggt gcgaatgcgt
     2521 cagcgagaag ttcatatccc tgtgttcggc gatgctgcag ttcgccttca gccgcaccag
     2581 cgacgcgcgg atgaaatatc cggacccgtt ctccctgatg agccgctgtg ccgattgtgt
     2641 ctcctcgaag tgatccgccg tcatccacaa tatgggacga agtgcgggct cgaacacctc
     2701 gaggatcggc aagcgggtcg gatggctgtg acgaaaatcg ggatcataga ccaggccgat
     2761 cgtctgggta tgctgatgca cctcgtaacg tttctgacgg accgagaagc tgttccactg
     2821 ttcttcggtc agatttttca cgagtacttt caggtcggcg gtatcgaggt cgccgatatg
     2881 aaagaaatta ccgggaaaat tcatgttcgg tactgtaacc ggggcaattc cggatctcat
     2941 cataaccgcc gcgccggagt tctgcagtca gggaaggatt gactgcgacg gagcgtattt
     3001 gacgaacggt gcgttgacct cgatgacttt cgcttcgcag attgccctgc ggtaatttgt
     3061 gtatataacc agcggttata aggctttgga aagcatctcg cactcgcccg catggcgggc
     3121 tcctacagcg gtgcaggttg tccgtttcgc aatatggagg cattgcccac gccgtccttc
     3181 cttttgctat tgttgctcgc aagtagccat aaatgaggct aacctttgcg cgacatctca
     3241 cgtaagcgga aacccgtcat gacgttcgag cgacgacgag gtttttcgca agcgacacca
     3301 cgctgtttcg ggggagacga aaatggcaag acgcagtaat cgtccgggct actccagttc
     3361 ggttgagcgg gttccgatgt tgacgccact ggctgcagcc gtgctaacgg cgctttaccc
     3421 gctaggcccg gcgtttgccc aggatgaggc ggtgccgagt gatgagccgg tcgaggaagt
     3481 gatcgtcacg ggctcacgca ttcgtagaga taccttctcc agtgcagcac cgatggaagt
     3541 cgtgctgtcc gaggccgcag cccccagggg cataaccgac gtggcctcgc tgctgcagac
     3601 gaccaccgtc gcagccggtg ctccgcaggt caccgcggca tcttcgagtg tgttcgtcga
     3661 gaacggtggc ctcggagcga gcacgctttc cattcgcggc ctcggcccga accgcacgct
     3721 tatcctgctc aatggccgcc gcgccggtcc ctcgggcgta cagggtggtg tttcgtcctt
     3781 cgatctgaac gccatcccga tgacggcgat cgagcgtgtc gagatcctga aggacggcgc
     3841 atcctcgatc tacggatccg atgccgttgc cggtgtcgtc aacatcatta ccaagaagga
     3901 ggacggggca gaattcgacg cgttcctctc agcgccacaa gagtccggcg gcgaacagat
     3961 gcgcctcagc ggctcgtggg gcaagtcttt cgatcgcggg cggatgcgcg ttacggctga
     4021 ttacaacaaa gtcgagatcc tgcagcgccg cgatcgggac tttttcgact gtgaggaaca
     4081 ttacatcttc gaccccgaaa ccggggaacg cgccgacctt atcgatcctc gcaccgggtc
     4141 gtacctgtgc gacgatctcc cttggggcca tgtctgggtc tacgactacg cggagagcta
     4201 cggcgacggc accaccaacg tcggcccgcc gacgtacctg ctgcaatacg attttgacgg
     4261 ttcgttggcg gcaaacggct tgccgccgct cgccgctccg acgagcccct atcacatgac
     4321 gacgccggcc ggctggtatc cggtcgcccg cggcgacgcc ctgtcggagt cgctcatcga
     4381 ttctcagcac ccgttgcatg gcacgcagac gctggttccc gagaacgagg ccatgaccgt
     4441 gttcctcgag ggcgaataca acctcaccga cagcgtcact gcctatgccg aggtcctgct
     4501 gaaccgccgc gagacggtgg acgtgggtta caagcagatc tggacctaca tctacaacta
     4561 cgattccgca ggtctggtcg atgacgactg gggtaccgac ccgttcagcg ccggctggac
     4621 cggcgcccaa tggctgagtc cgctgtcgat taccgaccac gccgaccagg aagtgacggt
     4681 ggactacggg cgcttcgtcg ccggactgac tggcgaatct ttgccggtgc tggaggactg
     4741 gagctgggac ctctcgttcc agtacagcaa gtccgacgga gaatacctca acgaacaggt
     4801 cctgacggac gcactggaag gccagtacaa cagaaccggc tcctgcgtag gaactaccac
     4861 gcccatttcg aaccgcccgt gcgtggacat tcagtggctg gacccggact tccttgccgg
     4921 cgatattccc caggaggtca agaactacct gttcgatgtc gagacaggca ataccgaata
     4981 cacccagtgg tcggtggaag ggttcatgac cggcgacctg ttcgagttac ccgcgggttc
     5041 ggttgccggc gcgatcggcg tccattaccg ggaagacgag atcctggaca cgccgggacc
     5101 cattacactt gtcagggacg ccaacggaca gcctgccctc gatgaggatg gccttacctt
     5161 cagtaatgcc tggcaggccg atgcagcagg gatcacagcg ggtgaggaca gtacctatgc
     5221 gatttttggc
//
LOCUS       DASUOM010000077         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1728150,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000077 DASUOM010000000
VERSION     DASUOM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728150"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..839
                     /locus_tag="VFY27_01650"
     CDS             9..839
                     /locus_tag="VFY27_01650"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX5764243.1"
                     /translation="MAVFRDRILMCPPDYFRVEYVINPWMAGHEGSLDKSLAKKQWQV
                     LRDALAEHAEIVTIEPQPDLPDMVFTANAGVVYGDKAVASHFMPLERRPEEPHFKKWF
                     RENGFELLDLDEKIGFEGAGDSLLDRGGPWLWTGYGFRTEIEAHEEIRKFFGLDLISI
                     RLTDSRFYHIDTCFCPLTGGFLMYHPPAFDFDSRMAIESRIPPHKRIVVDTADAGNFA
                     CNAVNIGHRVFLNKASEPLKANLLMAGFTVQEIDLSEFLKAGGSAKCLTLKVAEPEYA
                     "
     gene            869..2680
                     /locus_tag="VFY27_01655"
     CDS             869..2680
                     /locus_tag="VFY27_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5764244.1"
                     /translation="MTAEPHRYRITPANPGAHLFEVTLRIAQPDPDGQLLSIPAWIPG
                     SYLVRDFARNVVAIRAQAGDRDLELIKVDKSSWQAEACHEPLTVTLEIYAYDLSVRGA
                     HLDTTHAYFNGPCVFPQVVGQGGRPCEVEICPPPKPFGLEWRVATSMRSRDAERYGFG
                     HYCADSYDELIDHPVEIANLLIAEFEAAGIPHAIAVRGHERVDMSRVSRDLATLCERH
                     IGLLGAPHDLDRYLFLVLVLPEGYGGLEHRWSSSLVCSRGDLPVRGESGTKNSYRKFL
                     RLCSHEYFHLWNVKRMKPSRFTPYDLRAEAHTGLLWVFEGITSYYDSLLLMRSGLITK
                     ESYLELLGVTITRVIRARGRFRQSVEESSFDAWTKFYKPDANTSNAVVSYYTKGSLIA
                     LSLDLTLRRESDGKHSLDEVMRECWKRYGETGEGMPERGLESIAQAFTGIDLREFFDR
                     YVRGTGDLPLQKLLREFGVTMHLRQQAGSDDCGGKPSLSENPPPPWLGLNLSAKTGRD
                     VVTVVHTDSPAEQAGIAPGDELVALNDVRLTAPNMDTRLREYHAGDRVKVTVFRNDSL
                     LHLKVRLAEPPEDTCWLELDNNRSAEAERHQSAWLSP"
BASE COUNT          589 a          948 c          893 g          511 t
ORIGIN      
        1 acagctaaat ggcggtattc cgcgaccgga tattgatgtg cccgccggac tacttccgcg
       61 tcgaatacgt catcaacccg tggatggccg ggcacgaagg atctctcgac aaatcgctgg
      121 cgaaaaaaca gtggcaggtc ctgagggacg cgctggccga acatgccgag atagtcacga
      181 tcgagccgca gcccgacctt ccggacatgg tgtttacggc gaatgccggc gtcgtctacg
      241 gcgacaaggc cgtcgcgagt catttcatgc cgctcgagag gcggccggag gaaccgcact
      301 tcaaaaaatg gttccgcgag aacggcttcg agcttctgga cctcgatgag aaaatcggtt
      361 tcgagggcgc cggcgactcc ctgctggatc gtggtggacc gtggctatgg accggttacg
      421 gctttcgcac cgagatcgag gcgcacgagg aaatccgcaa gttctttggc ctcgatctca
      481 tctcgattcg gcttaccgac tcgcgcttct atcacatcga cacttgcttc tgcccgctga
      541 cgggcggctt cctcatgtac catccgccgg cgttcgactt cgactcgcgc atggccatcg
      601 agtcgcgcat cccgccgcac aagcgcatcg tcgtcgacac tgccgacgcc ggcaacttcg
      661 cctgcaacgc ggtcaacatc gggcatcggg tgttcctcaa caaggcttcc gaaccgctga
      721 aggccaacct cctgatggcg gggttcacgg tacaggaaat cgacctgtcc gagtttctga
      781 aggccggcgg ctcggccaaa tgccttacgc tcaaagttgc ggagccggag tacgcctaag
      841 gaatgttcgt gcagttgatc gaacctcaat gaccgccgag ccgcaccgtt accgcattac
      901 tcccgcgaac cccggcgcgc acctgttcga agtaacgttg cgtatcgcgc agccagaccc
      961 ggacgggcag cttctctcga taccagcctg gattccgggc agttacctgg tgcgggactt
     1021 tgcacgcaac gtcgttgcca tacgcgcgca ggcgggagat cgtgacctcg aactgatcaa
     1081 ggtcgacaaa agcagctggc aggcagaggc ctgtcatgag ccgctgaccg tcacgctcga
     1141 aatctacgcg tacgacctga gcgtacgcgg cgcgcacctc gatacgacgc atgcctactt
     1201 caacgggcca tgcgtgttcc cgcaggtggt cgggcagggg ggcaggcctt gcgaggtgga
     1261 gatatgcccg ccgcccaaac cgttcggcct cgaatggcgg gtggccacct ccatgcgctc
     1321 acgcgatgcg gaacgctacg gcttcgggca ctactgcgcg gacagctacg acgagctgat
     1381 cgaccacccg gtggaaatcg ccaaccttct catcgccgag ttcgaggccg ccggcattcc
     1441 acacgcgatc gcagtacggg gtcatgagcg ggtcgacatg tcgcgcgttt cccgcgacct
     1501 ggcgacgctg tgcgagaggc acattggcct gctcggcgcg ccgcacgatc tcgatcgata
     1561 cctgttcctg gtgctcgtgc ttccggaagg ttacggcggc ctcgagcatc gctggtcgtc
     1621 cagcctcgtc tgcagtcgcg gcgatctccc ggtgcgcggc gagagcggca cgaagaacag
     1681 ttaccgcaag ttcctgcggc tctgcagcca cgagtatttc cacctgtgga acgtcaagcg
     1741 gatgaagccc tcgcgcttca cgccgtacga cctgcgtgcg gaagcgcaca cggggctcct
     1801 gtgggtattc gagggaatca cctcgtacta cgactcgctg ctactcatgc gctccggcct
     1861 catcacgaag gaaagctacc tggaactgtt gggcgtcacc atcacgcgcg tcatacgcgc
     1921 gcgcggacgt ttccgtcaaa gcgtggaaga gtcgagcttc gacgcgtgga cgaagttcta
     1981 caaaccggac gccaacacca gcaacgccgt cgtaagctat tacaccaagg gttcgctgat
     2041 tgcgctgtcg ctggacctga cgctgcggcg tgaaagcgac ggcaagcatt cgctggacga
     2101 ggtcatgcgc gaatgctgga agaggtacgg cgagacaggg gagggcatgc cggagcgcgg
     2161 cctggaatcg atcgcgcagg cgtttaccgg gatcgacttg cgagagtttt tcgaccgcta
     2221 cgtccgcggc accggcgacc tgccgctgca gaaattgctg cgggaattcg gtgtcacgat
     2281 gcacctgcgc cagcaggcag ggagcgacga ttgcggcggc aagccttcgt tgtcggagaa
     2341 cccgccgccg ccgtggttgg ggctgaacct gtcggctaaa acgggaagag acgtcgtcac
     2401 ggtggtgcac accgacagtc ccgcggaaca ggccggcatt gcccccggcg atgaactggt
     2461 ggcgctgaat gacgttcgcc tgacggcgcc gaacatggac acgcggctgc gcgaatatca
     2521 cgcgggcgac cgcgtaaaag tcaccgtgtt ccgcaacgat agcctgttgc atctcaaggt
     2581 gcgtttggcg gaacctcccg aagacacctg ctggctcgag ttggacaaca accgcagcgc
     2641 cgaagccgaa cgccaccaat ccgcctggct aagcccgtag gagcccgccg tgcgggcgag
     2701 ttagacatac cgtcagcgaa gtcgcccgca cggcgggccc agcttgctat cagcgcgctg
     2761 cgggagcgcc gcgacggaac aatccatccc cttaaaccgg ccggtgccgc gagatcaccg
     2821 caccgccaac catcaaccct cctccttaaa cccttccacc ctcttgtacg caacatacga
     2881 gtaaaccacc ggcaccaaca cgatcacccc caccatcacc aggaacacca ccggcggaac
     2941 c
//
LOCUS       DASUOM010000078         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_280085,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000078 DASUOM010000000
VERSION     DASUOM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280085"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFY27_01660"
     CDS             complement(<1..258)
                     /locus_tag="VFY27_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764245.1"
                     /translation="MSTPQRSVLVLHEALRADARPDELDVLVQVREISEAIEQLHWRA
                     NTLEVDLDLKLALERLKDRNPACVFNLVESLQGRGELIGLVP"
     gene            complement(266..1291)
                     /locus_tag="VFY27_01665"
     CDS             complement(266..1291)
                     /locus_tag="VFY27_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764246.1"
                     /translation="MRRPNRLRIGITYDLRETWMEAGYSEVDTAEFDRRDTIDSIAAA
                     LETLGYDTDPIGNLQQLTRRLVAGDRWDMVFNICEGMHGWGREAQVPAILDAYQVPYV
                     FSDPLVCSLTLHKGMTKDVVRAAGVATPDFVVVNEPGSVRHIKLPFPLFAKPVAEGTG
                     KGVSCDSKINNAADLQAVCRQLLKEHRQPVLVERFLPGREFTVGILGTGRKATAIGTL
                     EIELLPGADAEVYSYRNKEECEELVEYRLLHEAKLKARVEALALRAWRTLGCRDAGRI
                     DVRLDEAGAVNFIEVNPLAGLHPQHSDLPIMASMTGMDYVSLIGGIMSSAKTRCRTST
                     RAVRLAA"
     gene            complement(1288..2592)
                     /locus_tag="VFY27_01670"
     CDS             complement(1288..2592)
                     /locus_tag="VFY27_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX5764247.1"
                     /translation="MKTAQRAEEPPEPGSRILPASVSIPSYLSNPPSNRLPKTIARRD
                     RAKFYVSERSADFLARYYPDATVDDWNNWRWQNRYRVRTLADLSRFIDLSEEESAAIK
                     RHTGALPVGITPYYASLLDRHDPLQPLRRTVVPVLGEYELSRGENEDPLGEDGHSPVP
                     GLVHRYPDRVLLLVTNFCSVYCRYCTRARMVGSAGERSVKKSDIEAALEYIEQTPVVR
                     DVLISGGDPLSLDDERLEYILRRLRRIRHVEFIRIGSKQPVVQPMRITPSLTRILKRY
                     HPLWMSLHFTHEAEITPEVTEACGRLADAGIPLGSQTVLLKGVNDELETLKKLMHGLL
                     RIRVKPYYLYQCDPISGSGHFRTSVAKGVELVRGLRGHTTGYAVPTFVVDAPNGGGKI
                     PVAPDYVLGYEKGDLLLRSYDGGTYRYPDESASLADRAEVSS"
     gene            2926..>3205
                     /locus_tag="VFY27_01675"
     CDS             2926..>3205
                     /locus_tag="VFY27_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764248.1"
                     /translation="MLPPQPKASMFPSLSNDPQTNRSLRHSLKDGIAFGTMTGTGETY
                     FSAFALFLKATTPQIGLLASLPPLLASFMQLVSAWLGRLTGQRKAIILA"
BASE COUNT          563 a         1007 c          999 g          636 t
ORIGIN      
        1 cggcaccagg cctatgagtt cgccgcggcc ctgcagcgac tccaccaggt tgaacacgca
       61 ggcaggattg cggtccttga gtcgctcgag cgcaagcttg aggtccaggt ccacttccag
      121 cgtgtttgcc ctccagtgca actgctcgat ggcctcgctg atctcgcgaa cctggaccag
      181 cacatccagc tcatcgggcc gggcgtcggc gcgcaacgcc tcgtgcaaaa cgaggacgct
      241 ccgctgagga gtgctcatcc ctgaatcagg cagcgaggcg cacggcgcgc gtcgaggtgc
      301 ggcagcgggt cttcgcggag ctcatgatgc cgccaatcag gctgacatag tccatccccg
      361 tcatcgatgc catgattggc aggtctgaat gttgcgggtg cagcccggcg agtggattga
      421 cctctatgaa gttgacggca ccggcctcgt cgaggcgcac gtcaatgcgg ccggcatccc
      481 ggcagccaag cgtccgccac gcgcggagcg ccagcgcctc gacccgcgcc ttgagcttcg
      541 cttcatgcag cagccggtac tcgaccagtt cttcgcattc ttccttgttg cgataggagt
      601 agacctctgc gtcggctccc ggcagcagtt cgatctccag ggtgccgata gcagttgcct
      661 tacgcccggt gcccagaatg ccgacggtga actccctgcc tggcaggaat cgctccacca
      721 gcaccggctg ccgatgctcc ttcagtaact ggcggcagac cgcctgcagg tccgccgcat
      781 tgttgatctt cgaatcgcag ctgacgcctt tgcccgtacc ctcggcgacc ggcttggcaa
      841 acagcgggaa cggcagcttg atatggcgta cgctgcccgg ctcgttcacg acgacgaagt
      901 caggagttgc gacacccgcg gcacgcacga cgtccttggt catgcctttg tgcagcgtca
      961 gcgagcagac caggggatcg gaaaacacgt acggtacctg gtaagcatcg agaattgccg
     1021 gaacctgtgc ctcgcggccc cagccatgca tgccttcgca gatattgaag accatatccc
     1081 agcggtcgcc ggctaccagc cgccgcgtca gttgctgcag gttgccgatc ggatctgtgt
     1141 cgtagcccag cgtttccaga gcagccgcaa tgctgtcgat ggtatcccgg cgatcgaact
     1201 ccgcggtgtc tacctcgctg tagcctgctt ccatccaggt ttcgcgcagg tcgtaggtga
     1261 tgccgatgcg cagtcgattc gggcgcctca tgacgatacc tccgcccggt ccgcaagcga
     1321 ggcgctttcg tccggatagc ggtacgtgcc gccgtcgtag ctccggagca gcaggtcgcc
     1381 tttctcgtat cccaggacat agtccggtgc gacgggtatc ttgccgccgc cgttcggcgc
     1441 atcgaccacg aacgtcggga cggcataacc ggtcgtgtgt ccgcgcagtc cccggaccag
     1501 ttcgacgccc ttcgccacgc tggtgcggaa gtggccggaa cccgagatcg ggtcgcactg
     1561 gtacaggtag tatggcttca cccgtatgcg caacaggccg tgcatgagct ttttcagcgt
     1621 ctccagctcg tcgttcacgc ctttcagcag tacggtctgg ctgccgaggg gaatgccggc
     1681 gtcggcgagg cgcccgcagg cctccgtgac ttcgggcgta atctccgctt cgtgggtgaa
     1741 gtgcaggctc atccacaacg ggtgatagcg cttcaggatg cgcgtcaggg agggcgtgat
     1801 gcgcatcggc tggaccaccg gctgcttgct gccaatccgg atgaactcga cgtgcctgat
     1861 gcgccgcagt cgccgcagga tgtattccag ccgctcgtcg tccaggctca gggggtcccc
     1921 accggaaatg agcacgtcgc gcacgacggg agtctgttcg atgtattcaa gtgcggcttc
     1981 gatgtccgac ttcttcacgc ttcgctcgcc ggcagagccg accatgcggg cacgcgtgca
     2041 gtagcggcag tagacggaac agaagttggt tacgagcagc aggacccggt cagggtaacg
     2101 gtgtacgagt cctggcaccg ggctgtgacc gtcctcgccc agcgggtctt cgttctcgcc
     2161 acgtgacagt tcgtactcgc cgaggacggg cacgacggtg cgccgcaacg gttgcagggg
     2221 gtcatgccgg tccagcaggc tcgcgtaata gggcgttatg ccgacgggca gcgcgccggt
     2281 atggcgcttg attgccgccg actcttcctc cgagagatcg atgaaccttg aaagatcggc
     2341 cagcgtgcgt acccgatagc ggttctgcca gcgccagttg ttccagtcgt cgaccgtcgc
     2401 gtcagggtaa tagcgcgcca ggaaatccgc cgaccgctcc gacacgtaaa acttcgcacg
     2461 atcgcgtcgg gcgatggtct tggggaggcg gtttgaaggc gggttggaaa ggtaggaagg
     2521 gatgctgacg gaggcgggga ggattctact tcccggttcc ggaggttctt ccgcgcgctg
     2581 ggcggttttc atcgacaaat cctttatgga gcagatgggc gacctgcaga tgaaaaaccg
     2641 acccggccct tcgagtcgtg cgggcaattg cccggcttga tttcgcggta atatatttgc
     2701 ggctgactaa atgtcaacca atttttgtcg agctgattca cacttctcaa cacaggtctt
     2761 tcgtcgcaaa aagcagacgc gttctcaagg ctcgcgcctg ctgtgaaatt cgacctgccg
     2821 accgtttcaa aaatagcatc ctgttcacgg cgtgtcgtaa cgcgcatgtg cggcagtcga
     2881 tggattcgcc gggcgctcag tgcccatagt gcgggcacct gaactttgct cccaccccaa
     2941 cccaaggcat cgatgtttcc gtccctctca aacgacccgc agacgaatcg ttcgctgcgg
     3001 cattcgctga aggacgggat cgcctttggc acgatgaccg ggacgggtga gacctacttc
     3061 tcggcgttcg cgctgttcct gaaggccacc acgccgcaga tcggcttgct cgcatcgttg
     3121 ccccccttgc tcgcatcctt catgcagctc gtctcggcgt ggctcggcag gctgacgggg
     3181 cagcgtaagg cgatcatcct ggcag
//
LOCUS       DASUOM010000079        10420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1145191,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000079 DASUOM010000000
VERSION     DASUOM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145191"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..2039
                     /locus_tag="VFY27_01680"
     CDS             60..2039
                     /locus_tag="VFY27_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5764249.1"
                     /translation="MKFRAIPQIRLCLLYFHTVVMAAFCLQSPSHADPYPLEYFALRD
                     VISNVRVSPDGKFLALMKIPNRDGNPIIEVYDAGDLGKAPFRVTADPMEITSFDWVSE
                     TNFVMSLRQKVRDRIDGFNQGVYDTRIAKVDVKRQKIEAFDELNVTISSVLPNKANKV
                     ILSFNPSGDRSSKVAEQFRPRAYYEFDVERATKELILQGQVALAQIEFDADGNPWTAR
                     GFDPGKAEFIWYVRTPGESKWQEIYRQGEDSFEVFEVEGFDEAQRNILFVRAHNGHDI
                     QGLWEFDTSTKSFGPLIYRRSDVDVVGVRYHSNSWTNHDTVVAVSYAKEKLFFEYFDG
                     VEEATYRQLEELVPSSHYVRIVSRSRDGETLVVENSGPRDPGTFYLLKDARFQAVGSK
                     QPLFKADDLADVEYITYAARDGRQIPAYLTVPYGEPPFPTIVLPHGGPFVQEIVGYDE
                     WGQLLANNGYLVLQPQYRGSHGYGLDFYTSAFRDGGQGGYKMQDDKDDGALYLVEQEL
                     ADRNRLALFGWSYGGYAALIAASRTPQIYQCVIAGAAVSDPIMQVNYYRDLLRGAQRD
                     EQLNMWRDSISPVKEIEKVNVPMLLIHGDVDQRVPPAHVRKYMKELERTGKDYRYVEL
                     KGADHFSNTLFYEHQIMLYESLIEYLRDDCGPGGL"
     gene            complement(2179..3972)
                     /gene="ilvB"
                     /locus_tag="VFY27_01685"
     CDS             complement(2179..3972)
                     /gene="ilvB"
                     /locus_tag="VFY27_01685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX5764250.1"
                     /translation="MSGADIVVQVIADEMVDTIFGYSGGAILPTYDAVFRYNERHRKP
                     DGKAAMPLIVPANEQGAGFMAAGYARASGKVGVVMVTSGPGATNTVTPVRDCMADSVP
                     IVVICGQVPTNAIGTDAFQEAPVSAIMGAVAKHVFLVTDPKRLEATVRTAFELARTGR
                     PGPVVVDIPKDVQNWEGEFTGCGYLPLTGYRNRWRAIVDNRLSDEACMRFYAMLAEAE
                     RPLIYAGGGVINSDATKAMRHLANAWGIPVVTTLMGLGASDTAHPLSLHMLGMHGAAF
                     ANYAVEDCDFLIAIGARFDDRVAGDPARFAPNARNIAHLDVDVSEIGKVKRVTWHHVG
                     LLKEDLQAIVAYGKRIGFAKEFGAWHAEIAELKSRHCMNYDRDSALIQPYAVIEEINR
                     IANGEAIITTGVGQHQMWAAQYFDFREPRLWLTSGSMGTMGFGLPAAIGAQFARPERL
                     VIDIDGDASIRMNIGELETVTTYKLPIKVVVLNNFGDGMVRQWQKLYYKGRMSASDKS
                     LHRKDFVRTAEADGFDFAQRLDRRENMPRVIREFISFPGPAFLEVIIDPDAGVYPMVG
                     PGQSYANMITGDYIESREPPAKDVLDPSEMF"
     gene            4164..4685
                     /gene="ilvN"
                     /locus_tag="VFY27_01690"
     CDS             4164..4685
                     /gene="ilvN"
                     /locus_tag="VFY27_01690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5764251.1"
                     /translation="MRHVISVLLQNEVGALTRMTGLFSTRGYNIDSLNVAPTDDPLVS
                     RVTLVISGSDAAIAQLNTQLAKLVDVVNIHDMTLGDHFERELVIVKLKLATGGLAKAL
                     NLAREFGAELLDDAQVSCTLQLTGRPAEVDAFVAAASGIGTLAAIARSGSVAVSKGEV
                     TLSSYARIHRLTG"
     gene            4693..6282
                     /gene="glpD"
                     /locus_tag="VFY27_01695"
     CDS             4693..6282
                     /gene="glpD"
                     /locus_tag="VFY27_01695"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX5764252.1"
                     /translation="MSPPTAYDVVIIGGGINGAGIARDAALRGLEVLLLEQNDFGSGT
                     SSWSSRLIHGGLRYLEHGEIPLVYESLRERRVLQEVAPHLVTRLRINIPVYDHSKRGL
                     LLLRAGMIAYDLLSLGKSLPNHRVLNREQFLRNEPGVNPSGLQGGVQYHDAQVAFAER
                     LVMENILAAHNAGAVVENYSPVTSIVVENGRVNHVVYRHGENGQKREAQAQIVVNASG
                     PWVDDVLSKTTLPGRKLIGGTKGSHIIVGAFDGSPKDAFYVEARSDGRPFFIIPWNGQ
                     YLIGTTDIRYDGDPAEAVASPEEIDYLLRETNRIFPQANLGTADIHYTYAGVRPLPRR
                     SAGPESAITRKHIIRRHEGAKGLWTVIGGKLTTYRSLAEDALDRIMRRSGLEAGACKT
                     REELLPGAAGLDGTETADRGLQIPEEARARLPRIYGSRTRDILDLMRNEPVLAAEAGP
                     GILAAEVVFAIRFEFARTLIDILHRRTMIGLSPDLGRSAAVEIAAVAARELGWSEAET
                     EQQLSALRAYNERLTPLTRPG"
     gene            complement(6403..7701)
                     /gene="icd"
                     /locus_tag="VFY27_01700"
     CDS             complement(6403..7701)
                     /gene="icd"
                     /locus_tag="VFY27_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5764253.1"
                     /translation="MTDATPTPPQGGARITIDNGNLAVPDQPIIPFIEGDGTGPDIWR
                     ATVRVLDAAVDKAYGGKRKIHWMEVYAGQKAYDLYNSWLPDQTVDACREYLVSIKGPL
                     TTPVGGGIRSLNVALRQLLDLYVCLRPVRWFNGVPSPVKDPGSVDMVIFRENTEDIYA
                     GIEFERGSQANEKFLTLFKENFPQEFDKIRFPGSCGIGLKAVSQEGTERLVRSAIEYA
                     ITNGRKSLTIVHKGNIMKFTEGAFRTWAYELADREYRDRTYTWNQWERTKAAKGADAA
                     NAEQDQALSSGKILVKDAIADIALQQVLTRPKEFDVIATLNLNGDYLSDALAAQVGGI
                     GIAPGGNINNVTGHAVFEATHGTAPKYAGLDVVNPGSLILSGEMMLRYLGWSEAADAI
                     VRAMDTTIGQKTVTYDFARLMEGATKVKCSEFATAMIGNM"
     gene            7871..10120
                     /locus_tag="VFY27_01705"
     CDS             7871..10120
                     /locus_tag="VFY27_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5764254.1"
                     /translation="MLNRMTRESICLAFLVAALAFAPGASAQVPANENANADNDELPR
                     IGLVLGGGGARGAAHIGVLHELERMRVPVHAIAGTSMGAIIGGLYASGMTPQELENLI
                     SNLDWAEVFQDAPRREDLSYRRKQDDVDFPVDFELGLNRGRLQLPKGLIQGQKLSPLL
                     RELTLDVAHISDFDELPTPFRALASDIETGESVVLSEGDLTLAMRASMSAPGIFAPVI
                     VDGRMLVDGGLVGNLPIETIREMDVDIIIAVDVEFPLYSREELDSAVGITGQVLTILI
                     HKETERQLATLDEDDILIRPQLGDFGSTNFADITDVVQPGAEATLAQASSLRRLALDE
                     ESYQRYQAGRRPSGDQNFLVDFVRVDDDGPLSARVLQSRLDTKAGETISPQALADDVA
                     RLYGLDLYEHVGYRLVREGEETGVVFETVPKSWGPNLLQFGLSLEDDFEGSTAFNVAG
                     RVTRAGINLLGAEWRTDLQLGTEPYLATEFYQPLSFDSKFFVAPRLRLEQTNLNGFAE
                     DLTVARYRIGEGEIALDLGRQLGRWGEFRVGAFRGTGNARVIVGQPDIPNIDFQTGGI
                     FASFGVDTLDDGQIPHSGMLIDATLTMSRPAFGADNNFDTVETTLTKVFTRGRHTFQM
                     GLDFSTTIQSDNLIQDFFPLGGFLRLSGLERGQITGPHAGLTRAIWYRRSGETGGGLF
                     DVPLYLGASLEAGNVWQSRSDISLESLIVNGSIFASLDTYFGPLFLAAGIAEGGQSSF
                     YLFLGTPPW"
     gene            complement(10145..>10420)
                     /locus_tag="VFY27_01710"
     CDS             complement(10145..>10420)
                     /locus_tag="VFY27_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764255.1"
                     /translation="HSRTTGSHAVDFVHRHNLVQPDAIPREKRFGIRVTLPPGDTFTR
                     LLGEDWERFHWYESEVERDAAFRQMAIRHGYYRKSDAPTQILQKLVR"
BASE COUNT         2001 a         3137 c         3210 g         2072 t
ORIGIN      
        1 gggtgcccgc ggacttatcc ccttgttgac aacagccgct ctgaagaaag tcaaaaacga
       61 tgaagttccg ggcaattcca cagattcgcc tttgccttct gtatttccac accgtggtga
      121 tggccgcttt ctgcctgcag agccccagtc acgccgatcc gtatcctctc gaatatttcg
      181 cgcttcgcga cgtcatcagc aacgtccgcg tgtctcccga tggcaagttc ctcgccctca
      241 tgaaaatccc gaatcgcgac gggaacccca tcatcgaggt atatgacgcc ggcgacctgg
      301 gaaaggcacc gttccgtgtg actgccgatc ccatggagat cacgagcttc gactgggtga
      361 gtgaaacgaa cttcgtcatg tccttgcgac agaaagttcg agacaggata gacggcttca
      421 accagggtgt ttacgacacc cggatcgcga aggtcgatgt caaacggcag aagatcgaag
      481 cgttcgacga gctgaatgtc acgatctcga gtgtcctgcc gaacaaggcc aacaaggtta
      541 ttctttcctt caatccgagc ggcgacagga gcagcaaagt agcagagcag ttcaggccac
      601 gagcatatta cgaattcgac gtcgaacgag ctacgaagga actgatactc cagggacaag
      661 tggcgttggc ccagatcgag ttcgacgccg acggtaatcc gtggactgcg cgcggcttcg
      721 atccgggaaa ggccgagttt atctggtacg tccgcacgcc gggtgagtcg aagtggcagg
      781 agatctaccg ccagggcgag gacagcttcg aggtcttcga ggtcgaaggg tttgacgagg
      841 cgcagcggaa tatcctcttc gtgcgagctc acaatggcca cgatatacag gggctgtggg
      901 aattcgacac cagcacgaag tccttcggtc cgctgatcta ccggcgttcc gatgtcgatg
      961 tcgtcggtgt tcgctaccac agcaacagct ggacgaacca cgacacagta gtggcggttt
     1021 cgtacgcgaa ggagaagcta ttcttcgagt acttcgatgg agtggaggag gcaacatacc
     1081 ggcagctaga ggagctggtg ccgagttccc actatgttcg catcgtaagc cggtcgcggg
     1141 acggagagac gctggttgtg gagaatagcg gcccgcgcga tccgggcacc ttctacctgc
     1201 tgaaggatgc caggttccag gcggtcggca gcaagcaacc cctgttcaag gcggacgatc
     1261 ttgccgatgt cgagtacata acgtacgcgg ctcgcgatgg ccggcagata ccggcctacc
     1321 tgacggtgcc ctacggcgag ccgccgtttc cgacaatcgt gctgccgcat ggcgggcctt
     1381 ttgtacaaga gatagtcggg tacgacgagt gggggcagct actggcgaac aatggctacc
     1441 tcgtattgca gccgcagtat cgcgggtctc atggatatgg cctggacttc tacacgagcg
     1501 cttttcgcga cggcggccag ggcggctaca agatgcagga tgacaaggat gacggcgcgt
     1561 tgtacctggt ggagcaggaa ctggccgacc ggaatcgcct cgccttgttc ggctggtcct
     1621 acggtggtta tgccgcgctc atcgcagcct cgcgaactcc ccagatctac cagtgtgtaa
     1681 ttgcgggcgc ggcggtgagc gacccgatca tgcaggtcaa ttattatcgt gacctcttgc
     1741 gtggcgcgca gcgcgacgag cagctgaaca tgtggcgtga cagcatctcc cctgtcaagg
     1801 agatcgagaa ggtcaacgtg ccgatgctcc tcattcacgg cgatgtggat caacgggtgc
     1861 cgccggcgca tgtgcggaaa tacatgaagg agctcgagcg aaccgggaag gactaccggt
     1921 acgtagaact caaaggggcc gaccacttct ccaacacact gttctacgag caccagatca
     1981 tgttgtacga gtccttgatc gaatacctga gggacgattg cggacccggg gggttgtagt
     2041 tgcggtgatc gctcaggtcg ggtcgcccgt aggacgggct cgtacagcgc ccgattccag
     2101 gcagcgcagg cggccggagc ggaacgctgt tggagctcgt cgtcgcacgg gcgactcacc
     2161 gcagcgtgag attcgcctct agaacatctc gctcggatcg aggacgtctt tcgccggagg
     2221 ttcgcggctt tcgatgtaat cgccggtgat catgttggca taactctgtc ccggccccac
     2281 catcgggtag accccggcat cgggatcgat gatgacttcg agaaatgccg gtccgggaaa
     2341 actgatgaac tcccggatga cgcgcggcat gttctctctc cggtcgaggc gttgcgcgaa
     2401 atcaaagccg tccgcttcgg ccgtcctcac gaagtccttc cggtgcagtg atttgtcgct
     2461 ggcagacatc cggcccttgt agtagagctt ctgccactgg cggaccatgc cgtcaccgaa
     2521 gttgttcagt accacgacct tgatcggcag tttgtatgtc gtgaccgttt cgagctcgcc
     2581 gatgttcatg cgaatgctgg catcgccatc gatgtcgatg accaggcgtt ccggtcgcgc
     2641 gaattgcgcg ccgatcgccg ccggcaagcc gaagcccatg gtgcccatgc tgcccgacgt
     2701 tagccacagg cgcggttccc tgaagtcgaa atactgcgca gcccacatct ggtgctggcc
     2761 aacgcccgtc gtgataatgg cctcaccgtt cgcgatgcga ttgatttcct cgatgaccgc
     2821 gtaaggctgg atcaatgcgc tgtcgcggtc gtagttcatg caatgcctgg acttgagttc
     2881 ggcgatctcg gcgtgccatg cgccaaactc tttcgcgaag cctatgcgct tgccgtacgc
     2941 gacaattgcc tgcaggtcct ccttgagcaa cccgacatgg tgccaggtga cgcgcttgac
     3001 cttgccaatc tcggaaacgt cgacgtcgag gtgtgcaatg tttcgcgcgt tcggtgcgaa
     3061 tcgcgcagga tcccccgcaa ccctgtcatc gaaacgggcg cctatcgcga tgagaaagtc
     3121 gcagtcttcg acggcgtagt tggcgaacgc ggcgccatgc atgcccagca tgtgcaacga
     3181 cagaggatgc gcagtgtcac tcgcgccaag gcccatgagc gtcgtgacga ccgggatgcc
     3241 ccacgcattg gcgaggtgcc gcattgcctt tgtggcatcg ctgttgatga cgccgccacc
     3301 ggcatagatg aggggccgtt ccgcttccgc gagcattgcg taaaaacgca tgcaggcctc
     3361 gtctgacagg cgattgtcga cgatggcccg ccaccggttg cgataccccg tcagcggaag
     3421 atagccgcag cccgtgaact caccttccca gttctggacg tcctttggaa tgtccactac
     3481 taccggacct ggccggccgg ttcgcgcaag ctcgaaggcg gtgcgtaccg tagcctcgag
     3541 ccgcttcgga tcggtcacca ggaagacgtg cttggcgacg gcgcccatga tcgcggaaac
     3601 gggcgcctcc tggaatgcat cggtaccgat tgcgttggta ggaacctggc cacagatgac
     3661 gacgatcggc acggagtccg ccatgcagtc tcgcaccggc gttacggtat tcgtcgctcc
     3721 cggccctgac gtcaccatta cgacgcccac cttgccgctg gcgcgtgcgt aaccggctgc
     3781 catgaaaccc gcgccctgtt cgtttgccgg aacgatcaat ggcatggctg ccttgccgtc
     3841 cggtttgcgg tggcgctcgt tgtagcgaaa cacggcgtcg taggtcggaa gaatggcgcc
     3901 gccgctataa ccgaagatgg tgtcgaccat ctcgtcggca atcacctgta cgacgatgtc
     3961 cgcgccactc atgcgagtgc cggttaaagg atgcagtggc tgggcgggcg cgtgtgagtg
     4021 tgaggtcatc gaaaaagctt tgcagggcag acttttgcga gtgtttacta aagtttattg
     4081 cattgcaata ctccataatc tgcgccacca tcgatcgcgc attcggtgga atggtttcat
     4141 cggaatcttc aggaagaagg aatttgcgtc acgtcatctc ggtactgctg caaaacgaag
     4201 tcggtgcact cacccgtatg accggcctgt tttccacccg cggctataac atcgattccc
     4261 tcaatgtcgc gcctacagac gatcctctcg tgtcccgggt tacgctggtc atatcgggct
     4321 cggatgcggc gatcgcccag ctcaataccc aactcgccaa actggtcgac gtcgtgaaca
     4381 ttcacgatat gacactcggc gatcatttcg agcgcgagct cgtgatcgta aagctcaagc
     4441 tggcgaccgg aggcctggcc aaggcgttga acctcgccag ggaattcggc gccgagctcc
     4501 tggacgacgc ccaggtgagc tgcacattac agctgaccgg ccggccggcc gaggtcgacg
     4561 cattcgtcgc ggcagcgtca gggataggca cgcttgcggc gatcgcccga agcggcagcg
     4621 tcgcggtctc caagggagag gtgacgctgt cctcctacgc ccgaatccac aggttgactg
     4681 gttaaatagc cggtgtcacc tccgaccgcc tacgatgttg tcatcatcgg cgggggaatc
     4741 aacggcgcgg gcatcgccag ggacgcggca ctcaggggcc tcgaggtcct gctccttgaa
     4801 cagaacgact tcggcagcgg cacgtccagc tggtcgagcc ggctgatcca tggggggttg
     4861 cgctacctcg agcacggcga aataccgctg gtctatgaat ccttgcgcga acgccgggta
     4921 ctgcaggagg ttgcgccgca tctcgtgacg agactgcgga tcaacatacc ggtctacgat
     4981 cacagcaagc gcggcttgct tctgttgcgc gcgggcatga tcgcctacga cctgctgtcg
     5041 ctcggcaaat cgctgccgaa tcacagggtg ctgaaccggg agcagttcct gcgcaacgag
     5101 ccgggcgtga acccgtcagg attacaaggt ggtgtgcagt accacgacgc gcaggttgcc
     5161 ttcgccgagc gactcgtcat ggagaacatc ctcgcggcac ataatgccgg agctgtagtc
     5221 gagaactaca gcccggttac atccatcgtg gtggagaacg ggcgggtaaa ccacgtcgtg
     5281 taccggcatg gggagaacgg gcagaagagg gaagcgcagg cgcagatcgt agtcaacgcg
     5341 tccggtccgt gggtggacga tgtgctgtcg aagaccaccc tgcccgggcg caagctgatc
     5401 ggcggcacca agggcagcca cattatcgtc ggagctttcg atggttcacc gaaagacgcg
     5461 ttctacgtcg aggcgcgatc ggacgggcgt cccttcttca tcatcccgtg gaacgggcag
     5521 tacctcatcg gtaccaccga cattcgttac gacggcgacc cggccgaggc tgtagcaagc
     5581 cccgaagaaa tcgattacct gctcagggaa acgaacagga tatttccgca ggcgaacctg
     5641 ggaacggcgg acatccacta cacctatgcg ggcgtcaggc cgctgccccg gcgcagtgcc
     5701 ggaccggaat ccgccattac ccgcaaacac atcatcaggc gtcacgaagg cgcgaaggga
     5761 ctgtggaccg tgatcggcgg caagctgacc acctatcgca gcctcgccga ggacgccctg
     5821 gaccgtatca tgcggagatc aggcctcgaa gccggcgcct gcaaaacccg cgaggaactg
     5881 cttcccggcg ctgccggact cgacggaact gagacagcag atcggggact gcagattccc
     5941 gaggaggcga gggctcgttt gccgcgcatc tatgggagca ggaccagaga catcctcgac
     6001 ctcatgcgca atgagcccgt gctcgccgcc gaggccggcc ccggaatcct ggccgcggaa
     6061 gtggtgtttg ccatccgctt cgagtttgcg cggaccctga tcgatatcct gcatcgccgc
     6121 accatgatcg gcctgtcgcc cgacctcggc cgctcggcgg ccgtggagat cgccgcggtt
     6181 gcagcgaggg agcttggatg gtccgaggcg gagacagaac agcaactgtc ggcacttcgc
     6241 gcctacaacg agcggctgac gccgttaacg cggccgggat agaagttgta ggagcccgcg
     6301 atgcgggcga gtgcgatatt ctgggagaac ctgcgaactc gcccgcatgg cgggctccta
     6361 cgggagcatc cccatgctgc gtcatatcag tcggtccagc gtttacatgt tcccgatcat
     6421 cgcggttgcg aactcgctgc acttgacctt ggtcgcacct tccatcagcc gcgcgaagtc
     6481 gtaggttacg gtcttctgcc cgatggtcgt gtccatggct cgcacgattg catcggcggc
     6541 ttcgctccag ccgaggtaac gcaacatcat ttcgccagat aaaatgaggg agccggggtt
     6601 taccacgtca aggccagcgt acttcggtgc cgtaccgtgc gtagcttcga aaacggcatg
     6661 tccggtgaca ttgttgatgt tgccgccggg cgcgatgccg atgcctccga cctgggcggc
     6721 gagcgcatcg gacaggtagt cgccgttcag gttcaaggta gcgatgacgt cgaactcctt
     6781 cggacgtgta agtacctgct gcagggcgat gtcggcaatc gcatccttga cgaggatctt
     6841 gccggagctc agggcctggt cctgttcggc atttgcagcg tctgctccct tggcggcttt
     6901 tgttcgctcc cactggttcc aggtgtaggt gcggtccctg tactcgcggt ccgcaagttc
     6961 gtaggcccag gttctgaagg caccctcggt gaacttcatg atgttgcctt tgtgcacgat
     7021 cgtcaggctc ttgcggccgt tcgtaatcgc atactcgatg gcggagcgca caagccgttc
     7081 cgttccttcc tgcgaaacgg ctttgagccc gatgccgcag gaccccggga agcggatctt
     7141 gtcgaactcc tgcgggaagt tttccttgaa cagggtgagg aatttttcgt tggcctggct
     7201 gccgcgctcg aattcgatgc ccgcgtagat gtcctcggta ttctcgcgaa atatcaccat
     7261 gtccacgctg cccggatctt tcacgggtga cggtacgccg ttgaaccagc gcaccgggcg
     7321 caggcagacg taaaggtcga gcagttggcg aagggcaacg ttgagagagc gaattccgcc
     7381 gccgacaggc gtcgtcagcg ggcccttgat ggagacgaga tattcgcggc aggcatcgac
     7441 agtctggtcc ggcagccagc tgttgtacaa gtcataggct ttctgcccgg catagacttc
     7501 catccagtgg attttgcgtt tgccgccata agccttgtcg acggccgcgt cgagaacccg
     7561 gaccgtcgca cgccatatat cgggcccggt gccgtcgcct tcgataaatg gaatgatcgg
     7621 ctggtcgggc accgccagat tgccgttgtc aatcgtaatt ctggcgccgc cttgcggcgg
     7681 tgtcggtgtg gcgtcggtca tgatttgctc cggtgagtgc tcgatggggc atcccgtccg
     7741 agaatttctg cacaggccgt gggtggcgta ttttctcccg gactcgtgca gacgctccct
     7801 cgtgcaggaa ccgcgtgcgt gacgccatta taatcggccc atctcgactt cgcccggcgg
     7861 ggagtacttc atgctgaatc gaatgacccg ggaaagcatc tgtcttgctt tcctggtcgc
     7921 agcgctcgct ttcgcgccgg gcgcatcggc acaggttccg gccaacgaga acgcgaacgc
     7981 cgacaatgat gaacttccgc gtataggcct ggtgctcggc ggcggaggtg cccgcggtgc
     8041 cgcgcacatc ggtgtactgc atgagctgga acggatgcgt gtgcctgttc acgcgattgc
     8101 cggcacgagc atgggcgcga ttatcggcgg actgtacgcg tccggcatga cgccgcagga
     8161 gcttgaaaac ctgatctcga acctggactg ggcggaggtt ttccaggatg cgccgcggag
     8221 ggaggacctg tcatatcggc gcaaacagga tgatgttgat tttcctgtcg acttcgaact
     8281 gggcctcaac cgcggccgcc tgcagcttcc aaagggactc atccaggggc agaaactgag
     8341 tccgttgttg cgcgagctta cccttgatgt cgcgcacatc agcgatttcg atgaactgcc
     8401 gacccctttt cgcgctctcg cttcggatat cgaaaccggc gaatcggtcg tgctctctga
     8461 aggagatctc accctcgcca tgcgcgccag catgtccgcg cctggaattt tcgcgccggt
     8521 gatcgtcgac ggaaggatgc tggtcgacgg cggtctcgtc ggcaacctcc cgatcgagac
     8581 gatcagggag atggacgtcg atatcatcat cgccgtcgac gtggagtttc cgctttactc
     8641 gcgcgaagag ctcgactccg cggtcggcat caccgggcag gtactgacga tactcatcca
     8701 taaggagacc gagcggcagc ttgctaccct ggacgaggac gacatcctga tacgccccca
     8761 gcttggcgat ttcggctcca cgaacttcgc cgacatcacg gacgtggtgc agccgggagc
     8821 tgaggccacg cttgcacagg cgtccagcct tcgacgtctc gcgctcgacg aagagtccta
     8881 ccagcgttac caggccggac gcagaccttc aggcgaccag aatttcctcg tcgatttcgt
     8941 ccgcgtcgac gacgatggcc cactctccgc acgcgtgctg cagtcccgcc tggacacgaa
     9001 agccggagaa acaatcagcc ctcaagccct ggcggacgac gtagcgcggc tctacggcct
     9061 ggacctttac gagcatgttg gttatcgatt ggtccgtgaa ggcgaagaga ccggcgtcgt
     9121 gttcgaaacc gtacccaaga gctggggtcc caacctcctg cagttcggcc tgtcgctcga
     9181 agacgatttc gaaggatcga cggcgttcaa cgtggcgggc agggtcaccc gcgccgggat
     9241 caacctgctt ggggcggagt ggcgcaccga cctgcaactc ggcaccgagc cttacctggc
     9301 gactgagttc taccagccct tgagcttcga ctccaaattc ttcgtcgcac cccggctgag
     9361 gctcgagcag acgaacctga atggattcgc ggaggacctc acggttgccc gctacaggat
     9421 aggcgaaggt gagatcgccc tcgatctcgg ccgtcagcta ggccgctggg gcgagttcag
     9481 ggtcggtgcg tttcgcggca cgggcaacgc ccgcgtgatt gtcggtcagc cggacatccc
     9541 gaacatcgac ttccagaccg gaggaatatt cgcaagcttc ggcgtagaca cgctcgatga
     9601 cgggcagatt ccccacagcg gcatgctcat cgacgcgact cttaccatgt cgcgcccggc
     9661 tttcggtgcg gacaacaact tcgacacggt cgaaaccacc ctgacgaagg tcttcacacg
     9721 cggcaggcat accttccaga tgggcctcga tttcagtacc accatccagt cggacaacct
     9781 catccaggac ttcttcccct taggcggctt cctgcgcctt tccggcctgg aacgcgggca
     9841 gatcaccggg cctcacgccg gccttacccg ggcgatctgg taccggcgct ccggtgaaac
     9901 gggcggaggg ctgttcgacg tgcccctcta tctcggtgcg tcgctcgaag ccgggaacgt
     9961 ctggcagtcg cggtcggaca tctcgctcga gtccctgatt gtcaacggca gcatctttgc
    10021 cagtctcgat acctacttcg gcccactttt cctggccgcc ggcatcgcgg aaggcgggca
    10081 gtccagcttc tacctgttcc tgggcacgcc gccctggtag tcaagagccc tgtgagtcct
    10141 gcagtcagcg gaccagtttc tgcaggatct gcgtcggtgc gtcgctcttc cggtagtagc
    10201 cgtggcggat ggccatctgg cgaaaagccg cgtcgcgttc tacttcgctt tcataccagt
    10261 ggaaccgctc ccaatcctcg ccgagcaagc gggtgaaggt gtctcctggc ggcagggtta
    10321 cgcggatgcc gaaacgcttc tcgcgcggga tggcatccgg ctgcaccagg ttgtgtctgt
    10381 gaacgaaatc cactgcatga ctgccggttg ttctcgaatg
//
LOCUS       DASUOM010000080         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_798129,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000080 DASUOM010000000
VERSION     DASUOM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798129"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFY27_01715"
     CDS             <1..359
                     /locus_tag="VFY27_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764256.1"
                     /translation="LLHEYERIGVDAAHRELDRITESVLLARSTVRNALGITRSAKPP
                     ITLDTVVNDLLEARERGELDVDDELAANLIAGLDPAIHLIKGDLASAPTLRRVALAGG
                     AATGPLVTLTGRGILN"
     gene            559..1248
                     /locus_tag="VFY27_01720"
     CDS             559..1248
                     /locus_tag="VFY27_01720"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5764257.1"
                     /translation="MKKPIAIAVGLALTGSMALAAQEQGSTEDYDPNRDIVTETESDR
                     GTTMEAPRQDTPVQSDYGTEPETQTESDMSTDPYGTDQSQSQTTDPAETQSQSQSDTY
                     GTESETESTQSASTWEDKSGTDMADMTADKLSGKSVVTATGEEIGEIDEVGYSATHQE
                     RVAVIEAGGFLGVGEKRIALPLSELEMGSDGNVKTSMDRDSIESQEEFDEAGFTADQT
                     DETDPMDDMSQ"
     gene            1514..2098
                     /locus_tag="VFY27_01725"
     CDS             1514..2098
                     /locus_tag="VFY27_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764258.1"
                     /translation="MSDERQTTREVKQQARSDFERGKQRAQEAAQDVAEQGKEKLMEQ
                     SERAASGVDDFADAVGSAASRLSELEHEGLADYANQLSSFLSDMSGTLRNKNVDELAR
                     DVRDVAQRNPALFLLGSVAIGLGLSRFAKASGKPRSRRETGGESEWRGDSGLRGDDEW
                     RGYGDEDFISDVARPEDTSTERQVTPDRTGGSGL"
     gene            2095..2565
                     /locus_tag="VFY27_01730"
     CDS             2095..2565
                     /locus_tag="VFY27_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5764259.1"
                     /translation="MNRRESGSTPTNRTGHTSPIIGDGENGAARYEDDSVPGLVKKLA
                     YDLSTLFNQELALAKAEIKSNVKDVGSGVSSIATGGAVLYAGLLFLLGGVMLLLTNWV
                     SLTTAAFIVGAVVTIIGAILLSSGKKKMSADSLTPDRTIDSVKKDADLARRKMQ"
     gene            2562..>2869
                     /locus_tag="VFY27_01735"
     CDS             2562..>2869
                     /locus_tag="VFY27_01735"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5764260.1"
                     /translation="MNERDVTRDTENGKDPKDPKDPARLERDIDRTRASLGRTVDALE
                     RRLSPGELVDQAMGMAREHGGEFATNLGRSVRNNPMPVILTGVGLAWMMASSNEPRAP
                     V"
BASE COUNT          681 a          819 c          913 g          456 t
ORIGIN      
        1 gcctgttaca tgagtacgag agaattggtg tcgacgccgc tcatcgtgaa ctggaccgga
       61 taacggagtc ggtgctcctt gcgcggagta cggtgcgcaa tgcactcggc attaccaggt
      121 cggcgaaacc gccgattacc ctggacacgg tcgtcaacga cctgctcgag gcacgcgagc
      181 gcggagagct cgacgtggac gatgaactcg cagcgaacct gatagcgggt ctcgatccgg
      241 ctatacacct gatcaagggc gatctcgcgt ccgcgcccac cctgcgcagg gtcgcgctcg
      301 caggcggcgc ggccacaggt ccgctcgtca cgctgacagg tcgcggcata ctcaactgaa
      361 agacagcctc gctgccccgt gcagcaggtg ccgtgcgttt cttccatccg cactttgtga
      421 aaattgcagt tactgcaaaa tctacctgtc tttttcggcc accttccacg cgattgctcg
      481 ccactctggc tggcacgacc tttgcaaccc accttggata ggcaagcgcc gataggttaa
      541 ataccaatgg aggtaacgat gaagaaacca atagcgatag cagtaggatt ggccctgacc
      601 ggcagcatgg cgttagccgc tcaggaacag ggatcgacag aggactacga tcctaatcgc
      661 gacatagtga ccgagacgga aagcgatcgg ggcacgacta tggaggcgcc tcgccaggat
      721 acccccgtcc agagcgacta cggcacggag cccgagacgc agaccgagag cgacatgagc
      781 accgacccgt atgggacgga ccaaagccaa agccagacca ccgacccggc cgaaacgcag
      841 agccagtcgc agagcgacac ctatggcacc gagagcgaga ccgagagcac gcagagcgct
      901 tcgacctggg aagacaaatc cggcacggac atggcggata tgactgccga caagctgagc
      961 ggcaaatcgg tcgtcaccgc aaccggcgag gaaattggcg agatagacga ggtgggttat
     1021 agcgcaacgc accaggagcg cgtggccgtg atcgaggccg gcggcttcct tggcgtcgga
     1081 gagaagcgca tcgccctgcc actgtccgag ctggagatgg gctctgacgg taacgtgaaa
     1141 accagcatgg acagggattc gatcgagtca caggaggagt tcgatgaggc aggcttcacc
     1201 gccgaccaga cggacgaaac ggaccccatg gacgacatga gccagtaaag agctcgcatc
     1261 gcgcgccggc agcgatcgct gcggcgcgag ttacaggaac cctgcagcgc ctttccggag
     1321 ttgcagggtt ttcttttttc atccgcaccg ccgtgctgcc gcaaaatgca ggcaatggca
     1381 cgatgattgc agagtctatt cagtcaacgc gaaggtcgcc tccgcgaacc cgagcgacac
     1441 ccctcccgga actgccagag aagagcggca gtcaggaatt tcaggcgaca aatcaccagc
     1501 agaggaaaac attatgagcg atgaacggca aacaacgagg gaggtaaagc agcaggccag
     1561 atctgacttc gagcgcggca agcagcgcgc tcaagaagct gcacaggatg tagccgaaca
     1621 aggtaaagag aaactgatgg agcaaagcga gcgcgccgcg tcgggtgtcg acgattttgc
     1681 cgatgcggtc gggtcggcgg cgtcgcgcct gagcgaactg gagcacgagg gcctggctga
     1741 ttacgccaac cagctttctt cattcctgag cgacatgtcc ggtaccttgc gcaacaagaa
     1801 tgtcgacgag cttgcacgtg acgtgcgcga cgtcgcgcaa cggaacccgg ctttgttcct
     1861 cctcggcagc gtagccatag gcctcggcct ttcccggttc gcaaaagcgt ccggtaagcc
     1921 gcgcagccga cgggagacag gcggcgagag cgaatggcgt ggcgattccg ggttgcgcgg
     1981 ggacgatgaa tggaggggct acggcgacga ggacttcata tccgacgttg cgcgccccga
     2041 ggatacttcc acagagaggc aggtgactcc ggacaggaca ggaggtagcg gactatgaac
     2101 aggcgagaat caggatcgac tcctacgaac cggacgggac acacgagccc gatcatcggc
     2161 gacggagaga acggagcggc aaggtacgag gacgactctg tgccgggtct ggtcaagaag
     2221 ctggcgtacg acctgtcgac gctcttcaac caggaacttg cgctggcgaa agcagaaatc
     2281 aagtcaaacg tgaaagacgt tggcagtggc gtgtcgtcaa tcgcgacggg cggcgcagtg
     2341 ctatacgcag gactgttgtt cctgctcggc ggcgtaatgc tgctgctcac taactgggtg
     2401 tctctcacca cggccgcgtt cattgtcggt gctgtagtga cgattatcgg cgccattctt
     2461 ctgagttccg ggaagaagaa gatgtcggcc gattccctga caccggatcg caccatcgac
     2521 tcggtcaaga aggacgccga tctggcaagg aggaaaatgc aatgaacgag cgcgacgtaa
     2581 cgagagatac ggaaaacggc aaggatccta aggatcctaa ggatcctgcg cggttggaac
     2641 gcgacatcga ccgtacacgt gccagcctgg gacgcacggt cgatgcgctc gaaagacggc
     2701 tgtctccggg agagctcgta gaccaggcca tgggcatggc gcgagaacac ggcggtgagt
     2761 tcgccacgaa cctcggccgg tccgtccgca acaacccgat gccggtaata ctgacaggag
     2821 tggggctggc atggatgatg gcatcctcaa acgaaccgcg agcgcctgt
//
LOCUS       DASUOM010000081         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1193129,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000081 DASUOM010000000
VERSION     DASUOM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193129"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..4478)
                     /locus_tag="VFY27_01740"
     CDS             complement(159..4478)
                     /locus_tag="VFY27_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764261.1"
                     /translation="MSDEFIEKIRQGFRDLGDKQNVVVKFIGHTDDAPLSGRIERIYG
                     TQVGLSKARARRVALAVQDRLKLPTAAIDSDGRGALHPLGSDETDRGRAMNRRVEVEF
                     WYDDPLQELPDEPQLCPDAPGAEMVTKVYDPPWGTIDHIEFNDGTPVISTGYAQVLER
                     AMADVAEKANVRLRFVGYTRNERLDRRTALVYGDDIGLSASRARRAMEAINGDMLLSD
                     SQREYEGRGYLYSDDVVNAGFIQGDTSYVKVQVVYDEPALLDDYDGIDITRLTRELSP
                     QNPLGLNLMRITVDGEPIDDPQRSSSDIQRCTDVAMDEADIQFGFDNLKSAPRLSISA
                     QPASVTIPESEPGAGGATVRFRMYTNYSHFIERAEVRIFDNGQSLQSEPLGVVDVEPD
                     GFAEWRPPAVQFRAPAHELAYVLRAYGSDGNFDETLPQPLWLIYGDAGEAPPEPAPEE
                     ATDSDSLLAAYGENGLARQNIGLSSGTVTVQGSGIPANHQVWVAGRPVPVDPKGNFVS
                     EEILPQGTHTVEVAVLDEQGNGELFLRDLEFRSNDWFYVGIADLTLSANDTEGPMASL
                     SGENTPYDNESTADGRLAFFVNGKFSEHWRLTASADTREGALGDIFSNFMEKSPDMLF
                     RRIDPDYYYPTFGDDGTVEELAPTMGKFFLRLSESDNYGQWGNFKVGYMQNELAQVDR
                     GLYGANLHYQSESTTSFGEQQFAVDTFAAEPGTVPSREEFRGTGGSLYFLRRQDILAG
                     SERVRIEIRDKASGLVTGALNLTPSLDYDIDYLQGRIMLSEPLASTADDSLLVRSGAL
                     SGDEAHLVVRYEYTPGFEDLDAVSTGGQVHYWMGEHLKLGLVSNVNELDDGDSKLHAA
                     DVTLRMSSESWLKLQTATSEGIVSNSQFSNDGGFGFYSQDPAAFTSAEAEAYRADVSV
                     ATNDLLRFGNGQITLYIQDAEAGYSAPGLATLLDTRNVGGTLRIPVLDRLSLQAKVDS
                     RVQEQGLETKAEEVNLNYSMSENWDVSAGYRRDERTDLSPLVPLTQEQGERTDAVVQL
                     GYNSRDDWNAYLFVQDTLSTTADRPENGRIGTGGSYRVSERLRLDAEISDGDLGKGGK
                     LGTNYLHSERTSMYVNYTLENERTDNGLRRTAGSEGNLVAGIKSRLTDSMSVYQEERY
                     QHSGATTGLTHATGISLAPTERLNIGMNTDIGTLQDVQTGAETDRRAAGIHVGYGFEA
                     LQLSSGIEYRHDDTEQLDQGISQRKTWLFRNSLKYQMNPSARLLGKFNHSDSASSLGA
                     FYDGGFTEAVVGYAYRPVSHDRLNALAKYTYFYNVPGADQVTLYNTAVEFIQKSHIAA
                     IDVSYDLLPDFSIGGKFAHRLSMVSLERENPDFFENDANLYVLRGDWQFRNVWDLVVE
                     GRILDMPDLNERREGMLLVVSRYVGDHTKVGVGYNFSDFSDDLTDLQFEHRGFFLNLT
                     GTL"
BASE COUNT          784 a         1408 c         1331 g          971 t
ORIGIN      
        1 ccggccggaa cctatgttga cggtctgatc gtaaccattc ttttctagct gaccttcctg
       61 caagcgcccc gctatcctgg gatggaccgc ccaaagctac ggggtttacg ctgcagttcg
      121 gactgcatcg ccagcccccg gatattgcgg cgatcagatc acagggtccc cgtcaggttg
      181 aggaagaagc cgcggtgctc gaattgcaga tccgtgaggt catcggagaa gtcactgaaa
      241 ttgtagccga ccccgacctt ggtgtgatct cctacgtagc gtgagaccac cagcagcatg
      301 ccctcgcgcc gctcgttgag gtcaggcata tcgaggattc gtccttcgac gacgaggtcc
      361 cagacgttcc ggaactgcca gtctcctcgc agcacgtaca ggtttgcatc gttctcgaaa
      421 aagtccgggt tctctctctc gaggctcacc atgctcaggc gatgcgcgaa cttgccgccg
      481 atcgaaaagt ccggcagcag atcgtagctg acatcgatgg ccgcaatatg gctcttctgt
      541 atgaactcca ccgcggtgtt atacagcgtg acctgatcgg cacccggaac gttgtagaag
      601 tacgtgtact tcgccagcgc attgagacgg tcgtgagaaa ccggccggta agcgtatccc
      661 actacagcct cggtgaatcc cccgtcatag aatgctccca gcgagcttgc gctgtccgaa
      721 tgattgaatt ttccgagcaa gcgtgctgac ggattcatct ggtatttcaa gctgttgcgg
      781 aacagccagg tctttcgctg cgagatgccc tgatcgagtt gctcggtatc atcgtgccgg
      841 tactcgatgc cgctcgagag ctgcagcgcc tcgaagccgt agccaacatg aattccggcc
      901 gccctgcgat ccgtttctgc accggtctgt acatcctgca gagtgccgat atccgtgttc
      961 ataccgatat tcagcctttc ggtcggcgca aggctgatac ccgttgcgtg tgtcagcccg
     1021 gtcgtcgcac cgctgtgctg gtacctttcc tcctggtaga cgctcatgct gtccgtgagc
     1081 ctggatttga tgcctgccac gaggttgcct tcgcttcccg cggtacggcg caagccattg
     1141 tcggtacgct cgttctccag tgtgtaattg acgtacatgc tggtgcgctc ggaatgaaga
     1201 tagttcgttc cgagtttgcc gcccttgccg agatctccgt cggatatctc tgcgtcgagt
     1261 cgcaggcgtt ccgagacccg gtaagatccg cctgtgccga ttcgcccgtt ttccggcctg
     1321 tctgcggtcg tcgagagcgt gtcctgcacg aacaggtagg cattccaatc gtctctcgag
     1381 ttatagccga gctgcaccac ggcgtcggtg cgttcgccct gctcctgcgt cagcggcacc
     1441 agcggtgaga gatcggtacg ttcatcccgc cgatacccgg cgctcacgtc ccagttttcc
     1501 gacatgctgt agttgagatt gacctcctcc gctttcgttt ccaggccctg ttcctgcacc
     1561 cggctgtcca ccttcgcttg cagtgacagg cggtccagga ccggtatgcg caaggtgccg
     1621 ccgacgttcc tggtatccag gagcgttgcc aggccgggag cggaatagcc ggcttccgca
     1681 tcctgtatat atagagtgat ctggccattc ccgaaccgga gcaagtcgtt tgttgccacg
     1741 ctgacgtcgg cacggtaagc ctccgcctcg gcactggtaa aggctgccgg atcctggctg
     1801 tagaagccga acccgccgtc attggagaac tgcgagttcg aaacgatacc ttcgctggtt
     1861 gccgtctgca gtttcagcca ggattcggaa ctcattcgga gcgttacgtc ggcagcgtgc
     1921 agtttgctgt cgccgtcatc cagttcgttg acgttggaga ccaggcctag cttgaggtgc
     1981 tcgcccatcc agtagtgaac ttgcccgccg gtggaaactg catccagatc ctcgaagccg
     2041 ggcgtgtatt cgtaacgcac caccaggtgc gcctcgtcac ccgacaacgc gccgctacgg
     2101 accagcaggc tgtcgtcggc agtcgacgcc agcggctcgg agagcatgat gcgtccctgc
     2161 aggtagtcta tgtcgtagtc cagcgaaggc gtgaggttga gcgcgccggt caccaggccc
     2221 gatgccttgt cccgtatttc gatgcggacg cgttccgaac ctgcaaggat gtcctggcgc
     2281 cgcaggaagt acagcgaacc acccgtgccg cgaaactctt cccggctcgg tacggtgccc
     2341 ggctcggcgg caaaggtatc gaccgcgaac tgctgctcgc cgaagctggt tgtcgactcc
     2401 gactggtaat gcaggttggc cccgtaaagt ccgcggtcca cctgtgccag ctcgttctgc
     2461 atgtaaccga ccttgaaatt tccccattgg ccgtagttat cgctttcgct tagcctcaag
     2521 aagaacttgc ccatggtcgg ggccagttcc tcgaccgtgc cgtcgtcacc gaaggtggga
     2581 tagtagtagt ccggatctat ccgccggaac agcatgtctg gcgacttttc catgaagttg
     2641 ctgaagatgt cgccgagtgc gccttcgcgc gtatccgcac tggccgtcag ccgccagtgc
     2701 tcgctgaatt tcccgttgac aaagaaggca aggcggccat ccgcggtcga ctcgttgtcg
     2761 tagggcgtgt tctcgccgct caacgaagcc atcggtcctt ccgtgtcgtt ggcagagagg
     2821 gtgagatcgg ctatcccgac gtagaaccag tcgttcgacc tgaactcgag atcgcgcagg
     2881 aacagctcgc cgttgccctg ctcgtcgagc accgccactt ccaccgtatg ggttccctgc
     2941 ggcaggatct cctccgagac gaagttgccc ttcggatcga ccggtaccgg ccgcccggca
     3001 acccacacct ggtgatttgc aggtatgccg ctgccctgga ccgtcaccgt gccgctgctc
     3061 aggccgatgt tctgccgcgc cagcccgttt tccccgtatg cggcgagcag cgaatcgctg
     3121 tccgtcgctt cctccggcgc gggttcgggc ggagcctccc cggcgtcgcc gtagatgagc
     3181 cagagcggct gcggcagtgt ctcgtcaaag ttgccgtcgg acccgtaggc ccgcagaaca
     3241 taagccagtt cgtgggccgg cgccctgaac tgaacggctg gcgggcgcca ttccgcaaac
     3301 ccgtccggct cgacatcgac gacgccaagc ggctccgact gcaacgattg tccattgtcg
     3361 aaaattcgaa cctcggcgcg ttcgatgaag tgtgagtagt tcgtgtacat ccggaaacgg
     3421 accgtcgcgc cgcctgctcc aggttcggac tcggggattg tcacgctggc cggttgtgcg
     3481 gaaatgctca ggcgcggcgc cgacttcagg ttgtcgaagc cgaactggat gtctgcctcg
     3541 tccattgcga cgtccgtgca ccgctggatg tccgatgaac ttcgctgcgg atcgtcgatc
     3601 ggttcgccgt ccacggtaat gcgcatcagg ttcaggccga gcgggttctg gggactcagt
     3661 tcccgcgtga ggcgcgtgat atcgatgccg tcatagtcat cgagtaacgc aggctcgtca
     3721 tagacgacct ggaccttcac ataagaagtg tcgccctgga tgaatccggc gttgacgaca
     3781 tcgtccgaat agaggtaacc gcgcccttcg tattcgcgct gggaatccga taacagcata
     3841 tccccattta tagcttccat cgcgcggcgc gcacgtgatg cggacaggcc gatgtcgtct
     3901 ccgtacacca gcgcagtccg gcgatccagc cgctcgttgc gggtgtagcc gacgaatcgc
     3961 aaccggacgt tggccttttc ggccacatcg gccatcgcgc gttccagtac ctgcgcgtag
     4021 ccggtggaaa tgaccggagt accgtcgttg aactcgatgt ggtcgatcgt tccccacggc
     4081 ggatcgtaaa ccttcgttac catctcggca ccgggcgcgt cggggcaaag ctggggttcg
     4141 tcgggcagct cctggagcgg gtcgtcgtac cagaattcga cctcgacgcg gcgattcatg
     4201 gcgcgtccgc ggtcagtctc gtccgaaccc agcgggtgca gtgcgccgcg gccgtcactg
     4261 tcgatcgcgg ccgtgggcag tttcaggcga tcctgcacgg ccagtgcaac acgcctcgcc
     4321 cgcgccttcg acagcccgac ctgcgtgccg taaatgcgct cgatgcgccc gctcaagggc
     4381 gcgtcatccg tatggccgat gaacttgacg acgacgttct gcttgtcgcc caggtcccgg
     4441 aatccctgcc ggatcttttc gatgaactcg tcgctcacgt cgatggattc ttcg
//
LOCUS       DASUOM010000082         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_789909,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000082 DASUOM010000000
VERSION     DASUOM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789909"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /locus_tag="VFY27_01745"
     CDS             <1..2639
                     /locus_tag="VFY27_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5764262.1"
                     /translation="IYKGKITRIEPSLEAAFVNYGADRHGFLPLKEISSEYFINDVAP
                     GAKPEIKHVLKEGQDIVVQIDKEERGNKGAALTTFVSLAGRFIVLKPNKSRSGGVSRR
                     IVGEDRDVAREALQDVTIPDGMSVILRTAGIDRNAEELQWDLDNLLAVWEAIKKVVVE
                     RPSPFLIYRESNAVIRALRDYLSNDIGEILIDDEATFAEAKEFVEQVMPQNLKKLKLY
                     TDAVPLFTRYQIETQIESAFAHSVTLPSGGSLVIDHTEALVSIDINSARATKGGDIEA
                     TALNTNLEAADEIGRQLRLRDLGGLVVIDFIDMGPQKNQREVENRLRDAVRQDRARVQ
                     IGKITRFGLLEMSRQRLRPSLGESSYMTCPRCSGIGNIRSVESLALAILRIIGEEARK
                     ERTSKVIAQLPVEVATYLLNEKRNWVQSLESRNDTQVILVANSALETPHYQIRRVRDD
                     QVELPENSGTSYKLAELAEEPDAVLAGVQERKVAEPAAVATITPSTPAPVRVIPESKG
                     PGLWSRLLQIFAGGEESAERSSRESSGRSGKKRDSRGGQRTRGRRSETQRGEPRSSRT
                     SKTGRKRGGKGSAEDSSRPSRGSSQRSGTDKSSALRNAAENGNVKSAEDGGFHDKKQQ
                     DAPTRSDSRNDGRDDTRRAPGKRRSRGGRRRKRADSGETAEASENQQRTEAGSTDGPG
                     KDEGSRERPSSRRRGGRNRTERTGESAPANPAAAERPAESIEPGPAAAATAGVQPTGG
                     AGEWRQTPPPARPDERETAWSSYEDTQPELPVMRPAPADRKSAAEPGHARPAAEVTGF
                     ENAPASAPPDRRAEAEPAPGTQLKTNGAEETAKPGGRLLPWEPMTEPMRKTKAESEPE
                     PARPEAAVAGKPLENVDRSESD"
     gene            complement(2673..3161)
                     /locus_tag="VFY27_01750"
     CDS             complement(2673..3161)
                     /locus_tag="VFY27_01750"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5764263.1"
                     /translation="MDPDKNVSVLFVCMGNICRSPTAEGFFRKVVTEAGLADRIHVES
                     AGTHAFHVGAAPDQRAQAAAARRGVTLMDIRARRIAETDFETFDYIFAMDKDNLGELL
                     ERAPEDHRDKVMLFLDFAGTGRAAEVPDPYYGGASGFELVLDLVEQASQRLLQRIQPR
                     LR"
BASE COUNT          778 a         1096 c         1101 g          510 t
ORIGIN      
        1 acatctacaa aggcaaaatc acacgcatcg agccgagtct cgaagcggcc ttcgtcaatt
       61 acggcgccga tcggcacggt ttcctgccgc tcaaggaaat ctcgagcgaa tatttcatca
      121 acgacgtcgc cccgggcgca aaaccggaga tcaagcatgt cctgaaggaa ggccaggaca
      181 tcgtcgtcca gatcgacaag gaagaacgcg gcaacaaggg agcggcactt accacgttcg
      241 tgagcctcgc cggccgcttc atcgtgctga agccgaacaa gtcccgctcc ggcggcgttt
      301 cccgtcgcat cgtaggtgaa gatcgtgacg tcgcccgcga agcattgcag gacgtcacca
      361 tcccggacgg catgagcgtc attctccgca cggccgggat cgaccggaat gccgaggaat
      421 tgcagtggga cctggacaac ctcctggcgg tgtgggaagc catcaagaag gtcgtggtag
      481 aacgcccctc gccatttctt atatatcgcg agagcaatgc cgtgatccgg gcgctgcgcg
      541 actacctgtc caacgacatc ggcgagattc tgatcgacga tgaagcgacc tttgcggaag
      601 cgaaggagtt cgtcgaacag gtcatgccgc agaacctcaa gaaactgaag ctctacaccg
      661 acgcggtgcc cctgttcact cgctaccaga tcgagacgca gatcgagtcc gctttcgcgc
      721 attcggtgac gctcccgtcc ggcggcagcc tggttatcga tcacaccgag gcgctggtat
      781 cgatcgacat caactccgcc cgcgccacca agggcggcga catcgaggcg accgcgctca
      841 acaccaacct ggaggcagcg gacgagatcg gccgccagct tcggcttcgc gaccttggcg
      901 gcctcgtagt gatcgatttc atcgacatgg gcccgcagaa aaaccagcgc gaggttgaaa
      961 accgcctgcg tgacgcggtg cgccaggatc gcgcccgggt gcagatagga aagatcaccc
     1021 gcttcgggct tttggaaatg tcgcggcaac gcctgcggcc gtcgctcggc gagtcgagtt
     1081 acatgacctg cccgcgctgc tccggcatcg gcaacattcg cagcgtcgaa tctcttgctt
     1141 tggcgatcct ccgcatcatc ggcgaagaag cccgcaagga aaggacatcg aaggttatcg
     1201 cgcaactgcc ggttgaagtg gctacttacc tgctgaacga aaagcgcaac tgggtacaga
     1261 gcctcgagag ccgcaacgac acccaggtca tactcgtcgc gaattcggca ctcgagacgc
     1321 cgcattacca gatccgccgc gtgcgggacg accaggtcga gctgcccgaa aactccggca
     1381 ccagctacaa gctggccgaa ctcgccgagg aaccggatgc agtcctggcc ggcgttcagg
     1441 aacgcaaagt ggcggagccc gcagccgtgg caacgattac gccgagcacg cccgcccctg
     1501 tgagggtaat acccgagagc aaggggcctg gcctttggag ccgcctcctc cagatattcg
     1561 caggcggcga ggagtccgcg gagcgaagct cccgcgaaag ctccggaagg tccgggaaga
     1621 agcgggacag ccgcggcggg cagaggacac gcgggcggcg tagcgaaacg caacgcggcg
     1681 agccgcgaag cagccggacg tcgaagacag gcaggaagcg cggcgggaag ggttctgccg
     1741 aagacagctc ccgtccctcc cggggcagct cgcaaagaag cgggaccgac aaaagcagcg
     1801 ccctgaggaa cgctgccgag aacggcaacg tcaagagcgc tgaggacggg ggattccacg
     1861 acaagaaaca gcaggatgcc cccactcgct ccgacagccg gaacgatggc cgcgacgaca
     1921 ctcgcagggc tccgggcaag cgccgcagtc gcggcgggcg acggcgtaag cgtgccgaca
     1981 gcggagagac ggcggaggcc tcagaaaacc agcagcgcac tgaagctgga tccacggacg
     2041 gacccggaaa ggacgaaggc agccgggaac gcccttcttc gaggcgaaga ggcggccgga
     2101 acaggacgga gcgtaccggg gagagtgctc ctgcgaaccc tgcggcagcc gaacgtccgg
     2161 ccgagagcat cgagccagga cctgccgccg ccgcgacggc gggtgtgcag ccaaccggcg
     2221 gcgccggtga atggcggcaa acaccgccac cggcccggcc tgatgaacgc gagacggcat
     2281 ggtcctctta cgaggatacc cagccggaac tgcccgtcat gcgtcccgct cctgcggaca
     2341 ggaaaagcgc agcggaacca ggacatgctc gacccgctgc agaggtgacc ggcttcgaga
     2401 atgcacccgc gtcggcgcct ccggaccggc gggctgaggc cgaacctgcg ccaggcaccc
     2461 agctgaaaac caacggtgcg gaggaaacgg caaagcccgg gggaaggctg ctgccgtggg
     2521 aaccaatgac ggaaccgatg cgtaagacga aagcagaatc ggagccggag cccgcgagac
     2581 ccgaagcggc tgtggctggg aagcccctcg agaacgtcga ccggagcgag tcggactagg
     2641 tctacgaggt aagcgcgcgg tccagggcgt cgttaacgca agcggggctg aatccgctgc
     2701 aataagcgct gcgacgcctg ctccaccagg tccaggacga gctcgaatcc cgacgccccg
     2761 ccgtagtagg ggtcgggaac ttcggccgcg cggcccgttc ccgcgaagtc caggaagagc
     2821 atgaccttgt cgcgatgatc ctccggggca cgctcgagaa gttcgcccag gttgtccttg
     2881 tccatcgcga agatgtagtc gaatgtctcg aaatccgtct ccgcaatccg ccgggcacgg
     2941 atatccatga gcgtcactcc ccggcgggca gcggcagcct gggctcgctg gtccggcgca
     3001 gctccaacgt gaaaagcatg ggtacccgcc gattcgacgt gtatccggtc ggcaagtccc
     3061 gcctccgtca ccaccttccg gaagaaacct tcggccgtgg gagaacggca gatgttgccc
     3121 atgcacacga aaagtacgct gacgtttttg tctggatcca ttaccaaata ttttcgatag
     3181 agtttgcgtc cgcgtacggt ccgcctggtt ccgtgcgcgt caaacacaga ttgtagccag
     3241 aactgtagcc ggacgggcag gaaatcccgt gcaacagatg ttagcagcaa atacaaacag
     3301 gccgcatcaa gaatggtgtt tcaagaagtc gaggcaattc tctcgtagca acatctattg
     3361 ctttcggagt cgcaacgtcg aaatcccacg gtcgcacaat tccgccaatt ttaccgcgga
     3421 gtcagatccg cgctccaatt gccttgcaaa gctgggcgat cgcggatcgg ccactacccc
     3481 ctgcc
//
LOCUS       DASUOM010000083         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1001520,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000083 DASUOM010000000
VERSION     DASUOM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001520"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFY27_01755"
     CDS             complement(<1..616)
                     /locus_tag="VFY27_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5764264.1"
                     /translation="MTAALTASLVVLWIVVILLGVAVVALARQVGVLHERVAPAGALL
                     MAGGARIGEAAPELRLKGIRGEDIRIGGKRNDRRHTLLFFLAPSCPVCKTLLPTLRSA
                     RKSESGWLDIVLASDGEMQEQLKFVERENLDVFPYVVSRDLGVSYRVGKLPYAYLIDD
                     QGVLRTGGLVNSREHLESLFNAKELEVGSVQEYLRREQTSEKHVA"
     gene            complement(613..1167)
                     /locus_tag="VFY27_01760"
     CDS             complement(613..1167)
                     /locus_tag="VFY27_01760"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX5764265.1"
                     /translation="MSALDPFYWHVIRWLVATVFAVACVHKLSSIAAFDNVVKDYALL
                     PSALAPLASRLLILCEIAVVAGLASGLRIPVAATLAMVLLAVYAAGMGINLARGRRDI
                     DCGCFGPAGSGENRHTLSGWLLLRNLSLIALTGLLLLPVADRALTWLDLFAIAAATAA
                     AFTAWLAGDQLMANAPRLQRRLSR"
     gene            complement(1171..2301)
                     /locus_tag="VFY27_01765"
     CDS             complement(1171..2301)
                     /locus_tag="VFY27_01765"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HEX5764266.1"
                     /translation="MATRRIVTAIFVALLAGTAAAEVPLDKPRVAALDAPKPHWFWAN
                     DVAFFNMADGRTYLYDADSGAMLGMLSTGIFYAKFETPNDYSMIYSPETYYSRHTRGE
                     RTDVVSFYDTRTLDIVDEVEIPPKRHSGLVNVGFSSLTDNERFLTIYNFTPAQSVSVV
                     DVEARTFIEEIATPGCALTYASGDSKFMTMCGDGRLMTISLDDQGKEASRLRSEPFFD
                     PAADPVMEKAVRREDTWYFISFEGEVHEVDVSGDEPAFSEPWSLVADDETGWRPGGLQ
                     LMAMHVVRAELAVLMHEHGGPGTHKDPGSEIWVYDLQSRERVRRIELAAPAVSINVTR
                     DAAPVLIASHGEPAIDIYNFRTGEHLRTITGVGQTPLYIQVP"
     gene            2494..>2826
                     /locus_tag="VFY27_01770"
     CDS             2494..>2826
                     /locus_tag="VFY27_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764267.1"
                     /translation="MSAPDREMPFMFELDALPVDFFDDPFPYYHELRQQHPFYRAADG
                     SLLLSRYTDVAQVYRNPQAFISDKHDLFRPKYGDSPLFEHHTTSLVFNDPPYHSRVRR
                     LIAGALKPA"
BASE COUNT          556 a          893 c          842 g          535 t
ORIGIN      
        1 acgcgacgtg cttctcgctc gtttgctccc ggcgcaggta ttcctgaacc gaacctactt
       61 cgagttcctt cgcgttgaag aggctctcca ggtgctcgcg cgagttaacc aggccgcccg
      121 tgcgtagcac gccctggtca tcgatgaggt atgcgtacgg aagcttgcct acccggtaac
      181 tgacacccag gtctcgcgac acgacgtaag ggaagacatc caggttctcg cgctcgacga
      241 acttcaactg ttcctgcatt tcgccgtcgc ttgccagcac gatatcgagc cacccggatt
      301 ctgacttgcg cgcagaacgc agggtaggca gcagcgtctt gcaaaccggg cacgaaggtg
      361 cgaggaagaa cagcagggtg tgtcgccggt cgttccgctt gccgccgatc ctgatgtcct
      421 ctccacgaat acccttaagc cttagttccg gagcggcttc gccgattcgt gcgccgccgg
      481 ccatcagcag cgcgccagcg ggcgcgaccc gttcgtgaag cacgccaacc tgtcgggcga
      541 gcgcgacgac ggcgacgccg agtagtatga ccacgatcca gagcaccacg agggatgccg
      601 taagggcagc ggtcatcgcg atagccgcct ttgcagccgc ggcgcgttcg ccatcagctg
      661 gtcgcctgcc aaccacgccg tgaaagcggc cgccgttgcc gcggcgatgg cgaacaggtc
      721 gagccacgtg agagcacgat cggcaaccgg caggagcagc aagccggtca acgcaataag
      781 cgacaggtta cgcagcaaca gccagccact cagggtgtgc cggttttcgc cgctgcctgc
      841 cggtccgaag cagccacagt cgatgtcgcg tcgcccgcgc gcgaggttga ttcccatgcc
      901 ggcggcatac accgccagca gcaccatcgc gagcgtcgcc gcgaccggta tccgcaggcc
      961 gctcgccagc cccgccacga ccgcgatctc gcacaggata agcaggcggg acgcaagcgg
     1021 cgccaaagcg gacggcagca aggcgtaatc cttcacaacg ttatcgaatg cggcgatgga
     1081 ggagagctta tgcacgcaag cgacggcgaa gaccgtcgcg accagccacc ggatgacatg
     1141 ccaatagaaa ggatcgagtg cggacaccgg tcagggaacc tggatgtaga ggggcgtctg
     1201 tccgacgccc gtgatggtgc gcaggtgttc gcccgtcctg aaattgtaaa tatcgattgc
     1261 gggctcgccg tgcgaagcaa tcagcaccgg cgccgcgtcc cgcgtcacgt tgattgaaac
     1321 ggccggcgcc gccagctcga tccttcgcac gcgctcgcgg ctttgcaggt cgtagaccca
     1381 gatttccgac cccgggtcct tgtgagtgcc cgggccgccg tgctcgtgca tcagcactgc
     1441 cagttccgca cgtaccacat gcatcgccat cagctgcagg ccgccgggcc gccagccggt
     1501 ttcatcgtcg gccaccaggc tccacggttc tgaaaatgcc ggttcgtcgc ccgagacgtc
     1561 gacttcgtgc acctcgcctt cgaacgatat gaagtaccaa gtgtcctccc ggcgcacggc
     1621 tttttccatg accgggtcgg cagcaggatc gaaaaacggt tcgctgcgca ggcgcgaagc
     1681 ctccttgccc tggtcatcga gcgagatcgt catcaggcgg ccgtcgccgc acattgtcat
     1741 gaacttggaa tctccggatg cataggtcag ggcacaaccg ggggtggcga tttcttcgat
     1801 gaacgtgcgt gcttccacat cgacaaccga aaccgactgc gcaggagtaa agttgtagat
     1861 ggtcagaaac cgttcattgt cggtcaggct gctgaagccc acgttgacga ggccagagtg
     1921 ccgtttcggt ggaatctcga cctcgtccac gatatccagg gtgcgggtgt cgtagaagct
     1981 gacgacatct gtgcgctctc cccgcgtgtg ccgggagtaa tacgtctccg gcgagtagat
     2041 catgctgtag tcgttcggag tctcgaactt ggcgtagaat attcccgtgc tcagcatgcc
     2101 cagcatggcg ccgctgtccg cgtcatacaa ataagtgcgg ccgtccgcca tgttaaagaa
     2161 tgcaacatcg ttagcccaga accagtgcgg ttttggggca tcgagggccg cgacccgcgg
     2221 cttatccagc ggcacctccg cggccgccgt gcctgcaagt aacgcaacaa aaatggcagt
     2281 gacgattctc ctggttgcca tgtttttttc tctcagcgcg cggcgtggca tgtggatgaa
     2341 ggtgtggcct gtataccaca ggcgaatagt ccattccaca aggacccggg cagcgcatcc
     2401 gcaaggttcg ctcccgtaca agcgtgcagc gaccagggct gctgatgtac ggtacgactc
     2461 aatcttcagc ttgtctttcg gggaagcagg attgtgagcg cacctgatcg ggaaatgcca
     2521 ttcatgttcg aactcgatgc tcttccggtc gattttttcg acgatccgtt cccttactac
     2581 catgagttgc gacagcagca cccgttttat cgcgcggccg atggatctct tttactcagc
     2641 cgatataccg atgtggcgca ggtataccgc aacccgcagg cattcatctc cgacaagcac
     2701 gacctgttcc ggccgaaata tggcgattcg cccctgttcg agcatcatac gaccagcctg
     2761 gtcttcaacg acccgccgta tcacagtcgt gtgcgtcgcc tgatcgcagg cgcgctgaaa
     2821 ccggcc
//
LOCUS       DASUOM010000084         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1738664,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000084 DASUOM010000000
VERSION     DASUOM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738664"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VFY27_01775"
     CDS             <1..1406
                     /locus_tag="VFY27_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5764268.1"
                     /translation="CSPTTSVSMTINGPAPMILAFFMNTAIDQQVEKYLKETGGWGAA
                     QEKIDAWFEGRERPRYEGELPAGNDGLGLKLLGISGDKLVSPAACREIRARTLASVRG
                     TVQADILKEDQAQNTCIFSTEFAMKMMGDVQQFFIENDVRNYYSVSISGYHIAEAGAN
                     PISQLAFTLSNGFTIVEYYLARGMHIDQFAPNLSFFFSNGMDPEYSVIGRVARRIWAR
                     AMRERYGASARSQMLKYHIQTSGRSLHAQEISFNDIRTTLQALYALFDNCNSLHTNAF
                     DEAITTPTEQSVRRAVAIQLIISKELGLNYCENPWQGSFIIDELTDLVEEAVYKEFDR
                     ISERGGVLGAMDTMYQRGKIQEESLYYESRKHDGSYPLIGVNTFLPRAGQEDEVRHQE
                     LIRSTAAEKDAQIRNVARFNARHADESPDAIARLQKVARSRSNVFAELMNTVKSSSLG
                     RISAALYEVGGEYRRNM"
     gene            1475..2323
                     /locus_tag="VFY27_01780"
     CDS             1475..2323
                     /locus_tag="VFY27_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5764269.1"
                     /translation="MKSYFFVTAAAISMVLVLTAVAGAVTGNYDRLAWCAAAIANLPL
                     PLFWLRLKFARVPRTSRDLPLLIFISAAGSVVALWQVLLSRSAEWQPAAIAVVGTGLL
                     LLYVFWYSRFGRVPNARLAIRNKLPEFTLADLDGREVKASEFLGTPTVWVFYRGNWDP
                     FCIAQVHEIASRHSELERLGVQLNLVSSQPEDRVRELAAELVPPVRFLVDRNAEAARI
                     LDIAEIYSLPPGAAGYAASGAMPTTIVTSASGTILFADETDNYRVRPEPDIYISILRR
                     AGAAAQ"
BASE COUNT          499 a          731 c          750 g          464 t
ORIGIN      
        1 tctgctcacc gacgacctct gtgtcgatga cgatcaacgg ccccgcgccg atgatcctcg
       61 cgttcttcat gaacacggcc atcgaccagc aggtggagaa atacctgaag gagaccggcg
      121 gatggggtgc ggcgcaggag aagatcgacg cctggttcga aggccgcgag cggccgcgtt
      181 acgagggtga gctgcctgcg ggaaatgatg gtctgggcct taagctgctg ggcatttccg
      241 gcgacaaact ggtatctcct gcggcttgtc gcgagatacg cgcacgaaca cttgcttccg
      301 tgcggggaac ggtgcaggcc gatatcctca aggaggacca ggcgcagaac acctgtattt
      361 tctccaccga gttcgcgatg aagatgatgg gcgacgtcca gcagttcttc atcgagaacg
      421 acgtccgcaa ctactacagc gtctcgatca gcgggtatca catcgcggaa gcaggcgcga
      481 atcccatcag ccagctcgcg tttaccctgt ccaacggctt cacgatcgtc gagtattacc
      541 tcgcgcgcgg catgcacatc gaccagttcg cgccgaacct gagcttcttc ttttccaacg
      601 gaatggatcc cgagtactcg gtgatcggca gggtggcgcg ccggatctgg gcgcgcgcca
      661 tgcgggaacg ctacggcgcc agcgcccgaa gccagatgct caagtaccac atccagacgt
      721 cgggacgcag cttgcacgcc caggaaatca gcttcaacga cattcgcacg acgctgcagg
      781 cgctgtatgc cctgttcgac aactgcaaca gcctgcatac gaacgcgttc gacgaggcga
      841 tcacaacgcc taccgaacaa tcggtacggc gcgcggttgc catccagctc atcatttcga
      901 aggagttggg cctgaattac tgcgagaatc cgtggcaggg ctcctttatc atcgatgagc
      961 tgaccgatct cgtcgaggaa gcggtgtaca aggaattcga ccggatctcg gaacgcggcg
     1021 gcgtcctggg agccatggat acgatgtacc agcgcggcaa gatccaggaa gaaagtctct
     1081 attacgaaag caggaagcat gatggctcct atcccctcat cggtgtgaac acgttcctgc
     1141 cgcgcgcggg ccaggaagac gaggtacggc atcaggaact gattcggtcc actgctgccg
     1201 aaaaggatgc gcagataagg aatgtggcaa gattcaatgc ccggcacgcc gacgaaagcc
     1261 cggacgccat tgcgcgcctg cagaaggtcg cgaggtcgcg cagcaacgtg tttgcagaac
     1321 tgatgaatac cgtcaagtcc agctcgctcg gccggatttc tgccgcgttg tacgaagtcg
     1381 gcggcgagta tcgacgcaat atgtgactcg agccgccgga ccgcccgggc tggtgcagcc
     1441 ggccggagaa tcccgcattg gagatcactt caggatgaaa tcctattttt tcgttacggc
     1501 agcggctatc agcatggtgc tcgtgctgac tgcggtcgcc ggagccgtca ccggcaatta
     1561 cgaccggctg gcctggtgcg cggcagccat cgccaacctg cccctgccgc tgttctggct
     1621 gcggctgaaa ttcgctcgcg tgccgagaac gagccgggac ctgccattgc tcatcttcat
     1681 atccgcggcc ggatccgtcg tcgcgctgtg gcaagtcctg ttgtcccgtt ccgcggagtg
     1741 gcaaccggcg gcgatagccg ttgtcggaac cgggttgctg ctcctctacg tgttctggta
     1801 ttcgaggttc ggccgcgtgc cgaacgcacg ccttgccatc cggaacaagc ttccggagtt
     1861 cacgctggcc gacctcgacg gcagggaagt aaaggcctcg gagttcctgg gaacgcccac
     1921 ggtgtgggtt ttctatcgcg gcaattggga tcccttctgc atcgcccagg tgcacgaaat
     1981 cgcgagccgc cactcggagc ttgagaggct tggcgtgcag ctgaacctgg tcagctcaca
     2041 gccggaggac cgtgtgcgtg aactggcggc ggagctggtt ccaccggtac ggttcctggt
     2101 cgatcgtaat gcagaggccg cccgaatcct ggacatcgcg gaaatatact ccctgcctcc
     2161 gggcgcggca ggctacgccg cttcgggcgc aatgcccacg acaatcgtaa cgagcgcttc
     2221 aggtacaatt ttgtttgcgg acgaaaccga caactatcgg gtgcggcccg aacccgacat
     2281 ctacatctcc attttgagac gcgcaggagc cgctgcccag tgaacgacca agtgaaaaac
     2341 gacgagctca ggagcttcat cgacaacccg acgctgtacc aggggactta tctcgagttc
     2401 ctggggtttc gcctgacggc atggaaagag ggttttgcgc ggct
//
LOCUS       DASUOM010000085         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1748163,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000085 DASUOM010000000
VERSION     DASUOM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748163"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFY27_01785"
     CDS             <1..1010
                     /locus_tag="VFY27_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX5764270.1"
                     /translation="LFDEDKSAFARYRELVFHTFSWPKLIRYELLVTFIGPIPGALGL
                     LLRKRLYRSLFRKVGKGVVFGRSLTLHHADRITLGDGVMLDRGATLDARGAGDAGIVI
                     GDRVIVNRGAAIQAKIGHIEIGNDCNIGSDVDVIAQGPIILKDGVSIACKATVAGGRY
                     VVERERDEANAKQRFTGGPIQIGSNTRIGMGAIIQDGVTIGDNAIVAPGAVVFEDVPP
                     DSVVWGNPARPVRNRSVTPAVEKNRSGAAAGSAAANTRLQQEVCKYLENDLFIEFGPD
                     GFSLSDSLLDSGVMDSLALVRLLLWTEEKFGVDLDFASLDPSEIDSVDRIVRRIAARR
                     Q"
     gene            1007..>2236
                     /locus_tag="VFY27_01790"
     CDS             1007..>2236
                     /locus_tag="VFY27_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5764271.1"
                     /translation="MTGNDLQPVFRQLQEHAASAPGARAIQSGTLAISYAELSSQIEA
                     TATALRQRMGVGRGDRVILLAEANANFVCTYFATHSIGAICVPLDPHVAAPRLRDIVE
                     RVSPRLIIAEKPPASHSSVTFDDLTSGGSGDSTAGSAPIDIEQTADILFTTGTTGRPK
                     GVILSHRALATACAHINEFIGIRADAVEVLPLPLSHSFGLGRVRCVLGAGGSLVLLPG
                     LVNASKIFEALENYRATGFASVPAGVAILLSDRGQALHRFSGQLQYIEIGSSAMPLEH
                     KRLLMDLLPNTRICMHYGLTEASRSAYLSFHDDNGKLESIGRPSPRVAMRVVDEAGGD
                     AEDGVNGQIEVRGDHLFTGYWQDAQLTGETLHDGWLRTGDLGRRDADGYYYLQARSSD
                     IINVGGRKVSPQEIEEVL"
BASE COUNT          445 a          681 c          686 g          424 t
ORIGIN      
        1 agctcttcga cgaggacaag tctgcgttcg cccgttacag ggaactggtc tttcatacgt
       61 tcagctggcc gaaattgatc cgctacgagc tgctcgtcac gttcattggc cctatccccg
      121 gggcgctggg gctcttactg cgcaagcgcc tgtaccgttc actgttcagg aaggtgggca
      181 aaggcgttgt tttcggccga agccttacac tccatcatgc cgaccggata acgctgggag
      241 acggcgtgat gctggaccgc ggtgcaaccc tcgacgcgcg cggcgccggg gacgccggta
      301 tagtcatcgg cgaccgggtg atcgtcaatc gcggcgccgc gatacaggcc aagataggtc
      361 atatcgaaat cggcaacgac tgcaacattg gatcggatgt cgacgttatc gcgcagggcc
      421 cgatcatcct taaggacggc gtttccatcg cctgcaaggc tacggtcgcc ggcggtcgct
      481 atgtagtcga aagggagcgt gacgaagcga atgcaaagca gcgctttaca ggtggtccga
      541 tccagatcgg cagcaatacg cgtattggca tgggagccat tatccaggac ggagtgacga
      601 tcggcgacaa cgccatagtc gctccaggcg ccgtcgtttt cgaggacgta ccccccgact
      661 cggtcgtctg gggtaacccg gcccgccccg tacgcaaccg gtctgtgaca cccgcagtag
      721 agaagaacag gagcggtgca gcagccggat cggctgccgc caataccagg ctacagcagg
      781 aagtgtgcaa gtacctcgaa aacgacctgt tcatcgagtt tggcccggac ggcttcagcc
      841 tcagcgattc cctcctggac tcgggcgtca tggattcttt ggcgttggta cgcctgttgc
      901 tgtggaccga ggaaaagttt ggagtagacc tcgacttcgc cagcctcgat ccatccgaaa
      961 tcgactcagt cgacagaatc gtcaggcgga ttgccgcacg caggcagtga cgggaaacga
     1021 cctgcaaccc gttttccggc agttacagga gcacgccgca agcgcgccgg gcgcacgcgc
     1081 tatccagagc ggaacgttgg cgatttccta cgcggaactt tcatcccaaa tagaggcgac
     1141 tgcaacagcg ctcaggcagc gcatgggagt cgggcgcggc gaccgtgtca tcctcctggc
     1201 tgaagccaat gccaacttcg tctgtacgta tttcgcgacg cacagcatcg gcgcaatctg
     1261 cgtgcctctc gacccgcacg tcgctgcgcc gcgcttgcgt gacattgtcg agcgcgtatc
     1321 gccacgactc atcatcgcag agaagccacc ggcatctcat tcaagcgtta cattcgacga
     1381 tctcacctcg ggcggtagtg gcgactccac tgccgggtcg gcgcccatag acatcgagca
     1441 gacggccgac atcctgttta caacgggtac gaccggccgg ccgaagggcg tcatcctgag
     1501 tcatcgcgcg cttgcgaccg cttgcgccca catcaatgag tttatcggca tacgggctga
     1561 tgcggtcgag gtgttgcctt tgccgctcag tcactcgttc ggcctgggac gcgtgcgctg
     1621 tgtcctcggg gccggtggca gcctggtgct tctgcccggg ctcgtcaacg cctctaagat
     1681 ttttgaagcg ctggaaaatt accgcgccac gggcttcgcc tccgtccctg ccggtgtcgc
     1741 catcctgctg agtgaccggg gccaggcatt gcaccggttt tcaggacaac tgcagtacat
     1801 cgagatcggc agttctgcaa tgccgctcga gcacaaacgc ctgctgatgg acctgctgcc
     1861 gaatacgcgg atctgcatgc actatgggct tacggaggcc agccgctccg cctacctgag
     1921 cttccacgac gacaatggaa agctcgagtc gatcggacgg ccaagcccgc gcgtcgcaat
     1981 gcgcgttgta gacgaagcgg gcggggacgc cgaagacggc gtgaacggcc agatcgaagt
     2041 tcgcggagac cacctgttca cgggctactg gcaggacgcc cagttaaccg gggaaacact
     2101 gcacgatgga tggttgcgca ccggcgacct tggccggcga gatgccgacg gctattacta
     2161 cctgcaggcc aggtccagtg acatcatcaa tgtcggagga cgcaaggttt ctccgcagga
     2221 aatagaggag gttctc
//
LOCUS       DASUOM010000086         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1607076,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000086 DASUOM010000000
VERSION     DASUOM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607076"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..1264)
                     /locus_tag="VFY27_01795"
     CDS             complement(503..1264)
                     /locus_tag="VFY27_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5764272.1"
                     /translation="MGELIDWSIMGPALAAGLLVLSTHVPLGQEVLNRGIIFIDLAIA
                     QIAGLGVIAAHGLDWEQDGFEVQVAAVSAALLAAAILNWTEKRWPRTQEALIGIFFIL
                     AATGGILLLANNPHGSEHLKELLVGQILWSTWTSLAPIAVLYAALLLVWFLWRDRIGA
                     LGFYVTFAIAVTASVQIVGIYLVFASLIIPAVATSRLRRGNRLFVSYAIGAAGFIAGI
                     ILSLAFDLPTGAIVVWAMAAVALVAGFSLSEPRAV"
     gene            complement(1264..2160)
                     /locus_tag="VFY27_01800"
     CDS             complement(1264..2160)
                     /locus_tag="VFY27_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5764273.1"
                     /translation="MIRYIVLLLVAVTPLSSRADIEIFACEPEWAALAEEIGGDLVDA
                     TSATTALQDPHYIQARPSLIARVRNADLVVCSGAQLEIGWLPALLQKANNRNVTPGSD
                     GYFEASMFVQRLDATGSVDRAQGDIHPQGNPHVQTNPHNLRLIAGALAERMAQLDSEN
                     AGAYQSGVADFTRRWDEAIASWEARAAPLKGLRVITHHKSWVYLERWLGLEEVANLEP
                     IPGLPPTAGHLSQLVRSFGDNGADIIIRAPYQDERASEWLHERTGIPAVLLPLSVGGT
                     EEATDLFTLFDDIIDRLLGATQ"
     gene            complement(2174..>2567)
                     /locus_tag="VFY27_01805"
     CDS             complement(2174..>2567)
                     /locus_tag="VFY27_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764274.1"
                     /translation="WRQRNFVFQSEYLWRNEDGAYALPGISTLPYDIDQRGWYAQAVY
                     QPFPRWRFGSRFDTLSMDDPGTAFAGTELALRDDDPRRLSFMVDWSNSEFSRLRLQYT
                     RDEAGASDDNQFGLQYTMSIGAHGAHSF"
BASE COUNT          472 a          789 c          807 g          499 t
ORIGIN      
        1 aggtagaacg aacgttcgag tttcatatta cgggccgtcg aggaactttt tcgagcgtgt
       61 gggctttgtg aaattggtgc ttggtttttc tggcgattga tacgatactc acctccgggc
      121 taacaacttt cagggaggtg atccaatgtc tagtgagatt acgagggtat cgatgcagcc
      181 tctggatatt gaatcgggta atggagcagc ctctgttatt tgattgagtc accaggatga
      241 gttccccgcg ctcctgatgt ccgtcgggga atagcatcac gaggttgaag atcttcgatc
      301 tcacggaagg ccgcggcgac gcggcctttc ttgtttggcg aagttacgct ccaggacgct
      361 gcctttcggg cgagccgctc ctgagaaatg gaatcctgca aagctcttcc aatccgctgc
      421 gtcggcacca cgtagcgctg taggagcctg ccatgcaggc gagttccgtg aatatggtcg
      481 cactcgcccg cccggcgggc tcctacacag cccttggctc ggagagggag aagcctgcta
      541 ctaacgctac tgccgccatc gcccagacga cgatggcgcc cgtcggaaga tcgaaggcca
      601 gcgacaggat gatccccgcg atgaagccgg cggcgccgat tgcgtagctg acgaacaggc
      661 gattcccccg gcgcaaccgt gaggtggcga ccgccgggat gatcaggctg gcaaaaacga
      721 ggtaaatgcc gacaatctgc accgaagccg tcacggcgat ggcgaaagtc acgtaaaacc
      781 cgagcgcacc gatcctgtcg cgccacagga accacaccag gagcagtgcc gcatatagca
      841 ccgctatcgg cgcgagcgac gtccacgtcg accagagaat ctgccccacc agcaattcct
      901 tcagatgctc gctgccgtgg gggttgttcg ccagcagcag gattccgccg gtagccgcga
      961 ggatgaagaa aatgccgatc agcgcttcct gcgttctcgg ccagcgcttc tccgtccagt
     1021 tgagtatcgc cgccgcgagc agtgccgcgc tgaccgccgc gacctgcact tcaaagccat
     1081 cctgctccca gtcgaggccg tgggcggcga tgacgccgag gccggcgatc tgcgcaatcg
     1141 cgaggtcgat gaaaatgatg ccgcggttca ggacttcctg gcctagcggc acgtgcgtgc
     1201 tgaggacgag gagcccggcc gcgagtgccg gacccatgat cgaccagtcg ataagttctc
     1261 ccatcactga gtcgctccca gcagccggtc gatgatgtcg tcgaagagcg tgaacaggtc
     1321 cgtcgcctcc tccgtgccgc cgacgctcag cggcaggagc acggccggaa tgccggtgcg
     1381 ttcgtgcagc cactccgacg ctcgttcatc ctggtaggga gccctgatga tgatgtcggc
     1441 gccgttgtca ccgaaactcc gcacgagttg gctcaggtgg ccagcggtcg ggggcagccc
     1501 cgggataggt tccaggttgg caacttcctc aagccccagc cagcgttcca ggtacaccca
     1561 ggacttgtga tgtgtgatga cccgcaaacc cttgagcggc gccgcgcggg cctcccaact
     1621 tgcgatcgcc tcgtcccagc gccgggtgaa atcggcgacg ccggattgat acgcgcccgc
     1681 gttttcgctg tcgagttgcg ccatgcgttc tgcgagcgct cccgctatga ggcgcaggtt
     1741 gtgcgggttc gtctgcacgt ggggattgcc ctgagggtgg atatcgcctt gcgcacgatc
     1801 gacgctaccg gttgcatcca ggcgttgcac gaacatgctg gcttcgaagt agccgtcgct
     1861 accgggtgtc acgttgcgat tatttgcctt ctgcagcagg gccggcagcc agccgatctc
     1921 cagttgcgcg ccgctgcaca ccacgagatc ggcattgcgc acccttgcga tcaggcttgg
     1981 ccgcgcctgg atgtagtgcg ggtcctgcag cgcggtcgtg gcgctcgtgg catccaccag
     2041 gtcgccgccg atttcctccg ccagcgcagc ccactccggt tcgcaggcga agatttcgat
     2101 atctgcacgc gatgacaacg gcgtgacggc aacgagcagc aatacgatgt atctgatcat
     2161 gatgtgctcc tgcctagaag gaatgcgcgc cgtgggcgcc gatgctcatg gtgtactgca
     2221 gcccgaactg gttgtcgtcg ctcgcacccg cctcgtcgcg cgtgtattgc aggcgcagcc
     2281 tgctgaactc gctgttcgac cagtccacca tgaagctcag gcgtcgcggg tcgtcgtcgc
     2341 gaagagcgag ttccgttccc gcgaatgccg tgccagggtc gtccattgac agggtgtcga
     2401 agcggctacc gaagcgccat cgggggaacg gctggtaaac ggcctgcgcg taccagcccc
     2461 gctggtcgat gtcgtatggc agcgtgctga tgccaggcag cgcataggca ccgtcctcgt
     2521 tgcgccacag gtattcgctt tggaaaacga agtttcgctg tcgccag
//
LOCUS       DASUOM010000087         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1365256,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000087 DASUOM010000000
VERSION     DASUOM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365256"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /gene="secA"
                     /locus_tag="VFY27_01810"
     CDS             complement(<1..1665)
                     /gene="secA"
                     /locus_tag="VFY27_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5764275.1"
                     /translation="MANFLTRIFGSRNLRLLRQYSKVVNRINALEEDLRALDAAQLGT
                     KTIEFKKRVADGESLDDLLPEAFAVAREAAFRTLGMRPFDVQLIGGMVLHDGNIAEMR
                     TGEGKTLVATLPAYLNALSGKGVHIVTVNEYLAARDAEWMGPVYRFLGLTVGVAKSGQ
                     THEEKVAAYAADITYATNNELGFDYLRDNLAFSLENKTQRELNFAVVDEVDSILIDEA
                     RTPLIISGPTEESTDLYQKINKLIPKLVEQKEEEGPGDYSKDEKAKQVHMTEEGHQRI
                     EELMTQAGLLSEGESLYDATNIRLVHHLNAALRAHVMFHRDVEYIVKNGEIVIVDEFT
                     GRTMPGRRWSDGLHQAVEAKEGVPVKQENQTVASITFQNYFRLYRSLSGMTGTADTEA
                     FEFQSIYGLEVIVIPTHKPMIRKDMSDLVYLTQEDKFKAIIEDITDCRERGQPVLVGT
                     TSIETSEYLSSLLRKKGIEHEVLNAKQHEREAIVVQQAGRPGAITIATNMAGRGTDIV
                     LGGNLEAELAAAGEDADKDAIRADWQQRLETVISAGGLHIIGTERHE"
     gene            complement(1874..>2318)
                     /locus_tag="VFY27_01815"
     CDS             complement(1874..>2318)
                     /locus_tag="VFY27_01815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5764276.1"
                     /translation="VLESVLINQQLVARVSPQGRPVTSGWMSSYFGKRTDPFTGKPAN
                     HKGVDFAGKAGAEVLAVADGVVTWSSKRYGYGQLVEINHGNGYATRYAHNSENLVAVG
                     DEVKKGETVALMGDTGRATGPNLHFEVLHQGKQVNPVNFIRQGAE"
BASE COUNT          383 a          709 c          713 g          513 t
ORIGIN      
        1 ctcgtggcgc tcggtgccga taatgtgcaa accgccggcc gagataactg tctcgaggcg
       61 ctgctgccag tccgcgcgga tggcatcctt gtccgcgtct tcgccggccg cggcgagttc
      121 tgcttcgagg ttgcccccca gcacgatgtc cgtaccgcgg cctgccatgt tcgtggcaat
      181 ggtgatcgca ccgggccgtc ccgcctgctg cactacgatc gcttcccgct cgtgctgctt
      241 ggcgttcagc acttcgtgct cgataccctt cttcctgagc agcgaagaaa ggtactcgga
      301 ggtttcgatg gaagtcgtgc cgaccaacac gggctggcca cgctcgcggc aatccgtgat
      361 gtcctcgatg atcgccttga acttgtcttc ctgcgtgaga tacaccaggt ccgacatgtc
      421 cttccggatc atcggcttgt gcgtcgggat cacgatgacc tcgagcccgt agatggactg
      481 gaactcgaat gcttcggtgt ccgccgtgcc ggtcatgccg gagagactcc ggtacaggcg
      541 gaaatagttc tggaaggtga tcgacgctac cgtctggttt tcctgcttga ccggcacccc
      601 ttccttcgct tcaaccgcct ggtgcagccc gtcggaccag cggcggcccg gcatcgtgcg
      661 gccggtgaac tcatcaacga tgacgatctc gccgttcttc acgatgtact cgacgtcgcg
      721 atggaacatg acgtgcgccc gcagcgccgc gttcaggtgg tgcacgaggc ggatattggt
      781 cgcgtcgtaa aggctttctc cctcgctgag caggccagcc tgcgtcatca gttcctcgat
      841 gcgctggtga ccttcctcgg tcatgtgcac ctgcttggcc ttctcgtcct tgctgtagtc
      901 gcccggcccc tcctcttcct tttgctcgac gagcttgggt atgagcttgt taatcttctg
      961 gtaaaggtcc gtgctttctt ccgtgggccc tgaaatgatc agcggcgtcc gggcttcgtc
     1021 gatcaggatg gagtcgactt cgtcgacgac ggcgaagttc agctcacgct gcgtcttgtt
     1081 ctccagcgag aaagccaggt tgtcgcgcag gtagtcgaag cccagttcgt tgttcgtcgc
     1141 atacgtgata tccgctgcgt acgcggccac cttctcctcg tgtgtctggc cgctctttgc
     1201 cacgccgacg gtcaggccga ggaaccggta tacaggaccc atccattccg cgtcacgagc
     1261 ggcgagatac tcgttgacgg tgacgatgtg gacacccttg ccgctcagcg cattcaggta
     1321 agcgggaaga gtcgcaacca gcgtcttgcc ttcaccggtg cgcatctctg cgatgttgcc
     1381 gtcgtgcagg accatgccgc cgatgagctg tacgtcgaag ggacgcatgc ccagcgtacg
     1441 gaaggcggcc tcgcgtgcca ccgcgaaagc ctcgggaagc aggtcatcga gcgactcgcc
     1501 atcggcgacg cgtttcttga actcgatggt cttggtgccg agctgtgcag cgtcgagcgc
     1561 acgcaggtcc tcttcgagtg cgttgatccg gttgactact ttggagtatt gccgcagcaa
     1621 ccgcaggttg cggctgccga aaattcgggt caagaaattg gccacttggc ctcctggctg
     1681 cacggtatgt gcgcggatac ctgcccccgg cgcgcacaaa gaccggtatt ttacggactg
     1741 gcctgacaaa tgcatcagtt gatgcgctta tattggtgta gccccgcgaa tctcaaggat
     1801 tttccggggc gagaatcgcg agacggcggg aaaaacgcag gagcctgcgt ctgcggattg
     1861 ggtgcggtcg gacttactcg gcgccctggc ggatgaaatt gaccgggttg acctgcttgc
     1921 cctggtgcag gacttcgaag tgcaggttcg ggccggtggc gcggccggtg tcgcccatta
     1981 gcgcgacggt ctcgcccttc ttcacctcgt cgccgacggc aaccaggttt tcggaattgt
     2041 gcgcgtagcg cgtggcgtaa ccattgccgt ggttgatttc gacgagttgg ccatatccgt
     2101 aacgcttgga agaccaggtg actacgccgt cagccaccgc cagtacttca gcgcctgcct
     2161 tgccagcgaa gtcaacgccc ttgtgatttg ccggcttgcc ggtaaaaggg tcagtgcgct
     2221 tgccgaagta cgaggacatc cagccggagg tcacgggccg tccctgcggc gagacacgcg
     2281 ccaccagttg ctggttgatc aggacgctct cgagcacg
//
LOCUS       DASUOM010000088         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_865968,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000088 DASUOM010000000
VERSION     DASUOM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865968"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFY27_01820"
     CDS             complement(<1..995)
                     /locus_tag="VFY27_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5764277.1"
                     /translation="MPHSSPLITTVVVALSLAWLLGVVAQRLKLPPLFGYLLAGIAIG
                     PFTPGFVADINLAGQLAELGVILLMFGVGLHFSWQDLLDVRGIAIPGALGQIASATLL
                     GVALATWFGWPLGAALIFGLALSVASTVVLLRALQERRLVQTDRGRIAVGWLIVEDIV
                     MVLALVLLPALAGLLGPEDVSQSDFTGIAAALGITVAKIVAFVAVMLLVGKRLIPWIL
                     HYVAHTGSRELFRLSVFVIALGTAFAAASLFDVSFALGAFFAGMVLSESALSQQAAQE
                     TLPLRDAFAVLFFVSVGMLFDPSVMVEEPLAVLATLAIIIVGKSAAAFGIMQLAG"
BASE COUNT          590 a          736 c          832 g          506 t
ORIGIN      
        1 tatccagcca gctgcatgat gccgaaggca gccgcggact ttccaacaat gatgattgcg
       61 agggtggcca gaaccgcgag cggctcttcg accattaccg atgggtcgaa caacattcca
      121 acagaaacga agaagaggac cgcaaatgcg tccctaagag gaagtgtttc ctgcgccgcc
      181 tgctgactta gtgcagactc gctcagcacc atgccagcga agaaggctcc aagtgcaaac
      241 gacacgtcga acaacgaggc tgccgcgaag gccgtcccga gcgctatgac gaagacggaa
      301 agccgaaaaa gctctcggct cccggtgtgg gcgacataat gcaagatcca tggaatcagg
      361 cgcttgccga ccagcagcat cacggcgacg aaggctacga tcttcgcgac ggttataccg
      421 agggcggcgg cgataccggt gaaatccgac tgagaaacat cctccggccc aagcaggcca
      481 gccaatgcgg gcaggagcac gagcgcgagc accatcacga tgtcttcgac gatgagccat
      541 ccgacagcga tgcgtccacg gtccgtctgg accagccgtc gttcttgcag cgcacgcagc
      601 aagaccactg tactcgcgac ggagagtgcc agaccgaaga taagggccgc accgagcggc
      661 caaccgaacc aagtggccag cgccacgccc agcaaggtgg cgctcgcgat ttgccccaga
      721 gcacccggaa tggcgatgcc gcgcacgtcc agcaggtctt gccatgaaaa atgcagacct
      781 acgccaaaca tcaacagaat gacgcctagt tcagccagtt gtcccgcaag attgatatcc
      841 gccacgaagc cgggcgtgaa aggccctatc gcgatgcccg ccaacaaata tccgaaaagc
      901 ggaggcaact tgaggcgctg cgccaccact cccagtagcc aagcgaggga tagggcgacc
      961 accaccgtcg taataagagg agagctatgt ggcatcgacc ctgctttcga aatcaggcgc
     1021 tcaaagccga gcttttcaga tcccttcttt agtacgaggc agggtaggcg aactcaacgc
     1081 gatatatgtc tgcttttggc accgagcaga catcccgaag ggcgatgctg acggcggctt
     1141 ttgaccccaa gcggttccga gaaacgacac tatcggaact agcgctagca acgcggatcg
     1201 cggaaacgcc gagcggcatt cagtagtcga tcccctgata ggctaggggc gaagtcaata
     1261 aaggaccccg ccgactggac agagggcggg gccaagcaac aggcgataac ttagacgcac
     1321 aggctcggca gcctgcggac gcagcctagc ccacattggg ccaatgcgta aaggatgagc
     1381 atcctgccta agtcgtggcg gttctttttg aggaaacgcc ctgttcccgc aagcaagcgc
     1441 tgggtgattt ccaggaccgt atcggtagtc ataggactct cttctgcggt ccaggcacga
     1501 gccggatcac cgcatcactg tcttctgtgc aagggcactg cgccaggttg gtggctcgcc
     1561 gagaaggcgc tgctacggtg tgcggtcgtc aagggcaata agcgcgttgc gtgcagcttt
     1621 ggcatcctgg acggtcatgg gctctcgcgt cgataggggc aggtctagcc aagccataca
     1681 gcggggatca aggtcgtcgt cagtgtcggg cgatgtcacc atcagagcgt tctccaagtc
     1741 tgcttgcgtt gctcttggta cctttcccct gccttggtta acgaggtctt gccctggcgc
     1801 cttatctttc gtggatcgtc gcccgtcgcg gtcgagcgcg cggccgatgg tagtagaaag
     1861 gaaggatggt gagcgaacaa acagcgtgcg agcgattggt gaggaggctg gtgagttacg
     1921 gtgagccgga aggcgttttc aatcggttgg gtgtgggatt gcttttgttg aggcgggtgt
     1981 ttcaagggca cttgtcgggg caggttgcgc ggaagctccc agtcgatggg ataaaagaag
     2041 ggttggtcgc aaacggacag gaggggcagt tttaccggca tttgtatttt catatcgggt
     2101 gccggctcct cggatggcct gggatgctcg cgagttggtt tatgcatcag gtggatgtga
     2161 ggcaggccgc gatgggacga atggaatcgg cggtcgagat caggggcaat gtggcggcgt
     2221 ttgcctgtgt ggaaattctc ttgaaccggt ccagacggag gatatcgcgg cgcgaggcgg
     2281 ctgaacggtt acgggagatc ctggcagaat gagggggggc acgcgccagg tcgttctgcc
     2341 agccattctg aaaggcttgc ggaatggttt cctcagaccg cggcccggct ccagtccggt
     2401 gtctcacacc gccattaggt ccattgaacc cataaatcac cacgccgttt cccaccgccc
     2461 tggcagggtg attcagagac atcgtcaggt cgacttagaa cgtccgctat tcattggttt
     2521 gtcggacatt cgtaagcggc gagggcggtt ccctgttttc tctgaaaaca ttccctgttg
     2581 gggtccaaaa tttccctgtt ccgcgtgtag ggaatttcac ccgcaatgcc ttgaggatgc
     2641 tggggcttca gcgcccagca acgg
//
LOCUS       DASUOM010000089         9636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1473417,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000089 DASUOM010000000
VERSION     DASUOM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473417"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..240)
                     /locus_tag="VFY27_01825"
     CDS             complement(1..240)
                     /locus_tag="VFY27_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX5764278.1"
                     /translation="MSDEQDISTDRDQSAPVGGEGTVPTFIPERRTEAGTASSMRNKK
                     REEQVTTVPVEIGGRNGPDPTRFGDWELKGRCIDF"
     gene            329..1192
                     /locus_tag="VFY27_01830"
     CDS             329..1192
                     /locus_tag="VFY27_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764279.1"
                     /translation="MFDIVRVSGPDARSFLQGQLTQDLARLDDAPSLRAAWCNPKGRV
                     IAVVRIIEAREGSDETCLSVPAGMADALVKRLLMFRFRARVDIALAGSGWGAFAVSDE
                     RDIAALDELGLLRRGGAGSAARNAGVVAVDTGAPAQCVEVYGPLAAMQEAGLALSRPL
                     TDAEWQFALINAGVPMIDAATTEKYTPHMLNLDCLDAISFTKGCYTGQEVVARTQHLG
                     KSKRRLMHYRTEEPLAGTGDKLRHDDREAGEVVNAVGEHLLAVVPVELRGSTLSLEGH
                     PATPVDLPYAL"
     gene            complement(1253..2758)
                     /gene="lysS"
                     /locus_tag="VFY27_01835"
     CDS             complement(1253..2758)
                     /gene="lysS"
                     /locus_tag="VFY27_01835"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5764280.1"
                     /translation="MRNDDVKHADENALIAERRRKLNELRAAGEAYPNDFRRNALAEE
                     LHCTFGPHDADKLPEVSPRVRVAGRMMAKRVMGKASFVKLQDRTGQIQVFLERDRLPE
                     GLYQDFRKWDIGDIVGAEGLLFKTKTGELTVKADNVRLLTKSLRPLPEKWHGLADMET
                     RYRQRYVDLIMNEKSRHVFRIRARIVAYIRAFMESQDFLEVETPMMQTLAGGAVARPF
                     TTHHNALDMQLYLRIAPELYLKRLIVGGLERVYEINRSFRNEGVSTQHNPEFTMLELY
                     RAYADYHDLMEMVEALLTGMSQAVLGTAVVDYQGEQYDLGKAFRRLTVEEAIVEFNPG
                     IDAARIRDRDYLQDFAEGVGARVEENYGSGKLQIEIFEQTCEAALRQPTFVVAYPVEV
                     SPLSRRNDDDPFVTDRFELFIGGREIANGFSELNDPEDQADRFRAQVKSRDAGDDEAM
                     VYDADYIRALEYGMPPTAGLGVGIDRLVMLFTDSPSIRDVLLFPHMRLETT"
     gene            complement(2759..3854)
                     /gene="prfB"
                     /locus_tag="VFY27_01840"
     CDS             complement(join(2759..3781,3783..3854))
                     /gene="prfB"
                     /locus_tag="VFY27_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787237.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5764281.1"
                     /translation="METTQIKQQIADLTERTEALRRYLDYENRAERLTEVRRELEQPD
                     VWNEPEKAQALGQERAALEKIVVNLDKLSRGLKDASELFELALDEDDKSTLDALVPEI
                     SGYAERIAELEFRRMFSGELDSANAFLDIQSGAGGTEAQDWAEMLLRMYLRWAEAHEF
                     ETEIIEASAGEVAGIKSATVRVVGEFAYGWLRTETGVHRLVRKSPFDSGNRRHTSFAS
                     VFVSPEIDDEIEIEIDPSDLRIDVYRASGAGGQHVNRTESAVRITHLPTNIVVQCQND
                     RSQHKNKATAMKQLKAKLYELELQTRRAKAAEVEDSKADVGWGSQIRSYVLDQSRIKD
                     LRTGVETGNTQAVLDGDVDRFIEASLKQGL"
     gene            complement(3930..5654)
                     /gene="recJ"
                     /locus_tag="VFY27_01845"
     CDS             complement(3930..5654)
                     /gene="recJ"
                     /locus_tag="VFY27_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5764282.1"
                     /translation="MTVTKERPVRRRESPDAAAVAAFSSLDPILARLYVTRGIAKEAE
                     LDLTLQRLVPVRTLDGVDTAVAVLMRSRRRRIIVIGDFDVDGATSTALVVRCLRDFGF
                     AEVSYLVPNRFEYGYGLTPEIVRVAAAMSPALLLTVDNGISSIEGVAEARRLGIDVVI
                     TDHHLPGAELPAAAAIVNPNLKGSTFGSPNLAGVGVAFYTMAALGNALQHEGRREAAR
                     IPARYLDLVALGTVADVVRLDQNNRILVMQGLRRIRAGSCTAGISALLAVSGRQRERV
                     NSTDLGFAAAPRLNAAGRLDDMSVGIECLLTDDSASATRYAEALDRINHERRDIETSM
                     RDQAFAFVDRLDQTGLPGCVCVYDESWHQGVVGLIASRVKERCHRPVIAFARDQDGQL
                     KGSARSVPGIHIRDLLEAVCTVRPGLVPRFGGHAMAAGLSLPAAHLEDFKVLVCQQLY
                     RLYPQADFSGALLSDGDLPATSMTLRFARVLRDGGPWGAGFPEPLWCGDFELVEQRIV
                     GELHLKLKVRPLDGNARIDAIAFNQASAAFRGSARIAYRLDVNEWLGVESPQLVVEQI
                     SRLASPTT"
     gene            complement(5670..6617)
                     /locus_tag="VFY27_01850"
     CDS             complement(5670..6617)
                     /locus_tag="VFY27_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764283.1"
                     /translation="MPLKKHAILVLPPAPHEGESPRVPLRSHRWLARSRVSSASGEIL
                     SQILSILAAPTPPEGLGALRLWGQTGAHPEGWVAAADPVFLEARLNHVVLHKLDEADL
                     DVAEVADIFAHLQENLAGNGDQGFSSVGKFGYVHRRRPMAVAHASPAVAQGNFPEDFL
                     PTGEQAKAHDRLQSEVQMCLYDSAVNRRRAIAGKPPVTSLWMWGGGIAPALPMMPLPP
                     LYANEPLCRGYWLAASAAVADWTGDLESCLSRSPDGFVAVLPAPAAAERSEQDDVHLA
                     FLRRMLRRGRLRRVTLLIGRGSRVDIGRFDALRLWRRKF"
     gene            6810..7226
                     /locus_tag="VFY27_01855"
     CDS             6810..7226
                     /locus_tag="VFY27_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX5764284.1"
                     /translation="MQFTREQISEITVRQVEPGRIRIGNAVFHNNVLLTVERAIRDWS
                     LEDIDDLDESALEAILEDAPEMIVVGTGWKPVFPSRELVFALARRGIGFETMDTPAAC
                     RTFNILINEGRRAAAALIVNGPVPAGASLAANVDRS"
     gene            complement(7260..8111)
                     /locus_tag="VFY27_01860"
     CDS             complement(7260..8111)
                     /locus_tag="VFY27_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5764285.1"
                     /translation="MRVDASAVSPHDAKRLYLNEVGTSRLLSAEEEVQIARRIRQGDE
                     AAWRRMIESNLRLVVKVARRYIDRGLPLLDLIEEGNLGLIHAVRKFDPERGFRFSTYA
                     TWWIRQTIERAIMNQTRTVRLPIHVIKDINSCLRAARYLRQVNGAAPTAAEIAQHLKR
                     EPSDVERLIALHERVSIGSAASDEDTTPLDMLADGPEAEPPQCVQQERVTAILDAWLL
                     KLDSKQRAVVEQRYGLHGHKRLTLEEIGREIGVTRERVRQIQLDALNNLKRMMESHGF
                     NGAALLD"
     gene            complement(8288..8974)
                     /locus_tag="VFY27_01865"
     CDS             complement(8288..8974)
                     /locus_tag="VFY27_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5764286.1"
                     /translation="MPFRLSLLLAALLAAACGPATRRDHSPDTHIVRSGETLFTIAWR
                     YGRDYRDLARWNRLGDGSLIYPGQVIQLDPPAGARAAAPRQPVSRPAPRRAEPLPPIP
                     AEPAPPWEWPTSGRVVVEFGGRPGSGTGILIDGKPGQPIEAAAAGRVVYSGGGLIGYG
                     QLIIIKHNDTYLSAYGHNASLLVREGEGIRKGQRIATMGEGPGREPRLHFEIRRNGKP
                     VDPRQYLPAR"
     gene            complement(9019..9603)
                     /locus_tag="VFY27_01870"
     CDS             complement(9019..9603)
                     /locus_tag="VFY27_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX5764287.1"
                     /translation="MRLFGPLYERVLQWSRHRHAERYLAGLSFAESSFFPIPPDVMLA
                     PMCLAERKRAWRYATVTTVASVLGGVAGYAIGFFLFEMIEPWLQTTHYWESYVAARAW
                     FDKWGVLAVFVAGFSPIPYKIFTIAAGAAVLTFPWFVVASFLGRGGRFYLVAGFIVAG
                     GERMAALLPRYVERIGWAVVAIAVVGVAFLMMKE"
BASE COUNT         1738 a         3070 c         2967 g         1861 t
ORIGIN      
        1 tcagaaatcg atgcatcttc ccttcaactc ccaatcgccg aagcgagtcg ggtcgggacc
       61 attccgcccc ccgatttcca ccggcactgt cgtcacctgc tcttcccgct ttttattcct
      121 catggacgaa gccgtgcctg cttcggtccg gcgctcggga atgaaagtgg gtacggttcc
      181 ctcgccgccg acaggcgccg attgatcgcg gtccgtggaa atgtcctgtt cgtcagacac
      241 ccgcggagta tgccagactg tgtgtcatga tgcagtcatg cgcccgcaat gtggttggca
      301 ctccaggtga tccgcgactt tgaacaggat gttcgacatc gtgcgcgtat ccgggcccga
      361 cgctcgctca ttcctgcagg gccagctcac gcaggacctt gcaaggctcg atgacgcccc
      421 gtccctgcgg gctgcctggt gcaatccgaa aggtcgtgtc attgcggtag tgcgaatcat
      481 cgaggcgcgg gagggcagcg acgagacctg tctctccgtg cctgcaggca tggccgacgc
      541 gttggtcaag cgtctgctga tgttcagatt ccgggcgcgg gtcgacatcg cacttgcggg
      601 cagcggatgg ggggctttcg ctgtgtccga tgagagggac atcgctgcgc tggacgaact
      661 gggtctccta cgccggggag gcgccggatc ggccgctcgc aacgccggcg tagtcgccgt
      721 agacacgggc gccccggcgc aatgcgtcga agtgtatggt ccattggcag ccatgcagga
      781 agcggggctc gcactctccc ggccattgac ggatgccgaa tggcaattcg cactcatcaa
      841 tgcaggagtg ccgatgatcg atgccgcaac aacggagaaa tacactccgc acatgctgaa
      901 cctcgactgc ctcgatgcca tcagtttcac caagggctgc tatacgggac aggaggtcgt
      961 ggcgcgcacc cagcatcttg gaaagagcaa gcggcgactc atgcattatc gaacagagga
     1021 gccgctcgca ggcaccggcg acaaactgcg ccacgatgac cgcgaagcgg gcgaagtagt
     1081 gaacgcagtg ggcgagcacc tgctcgcggt cgtacccgta gaacttcgcg ggtcgacgct
     1141 gtcgctcgaa ggacatccag cgaccccggt cgacctccca tacgcgctgt aggaacccgc
     1201 catgcgggcg agtgcgattc acgcccagcg aactcgccgg catggccggc tcctacgtgg
     1261 tttccaaacg catgtgcggg aagagcagca cgtcgcgaat cgaaggcgaa tccgtgaaca
     1321 gcatgacgag ccggtcgatc cccactccga gccccgcggt gggcggcata ccgtactcga
     1381 gcgcgcggat gtaatcggcg tcgtagacca tcgcctcgtc gtctccggcg tcgcggcttt
     1441 tgacctgtgc cctgaatctg tcagcctggt cttcgggatc gttcagctct gagaatccat
     1501 ttgcgatttc tcggcctccg atgaagagct cgaaacggtc ggtgacgaag ggatcgtcgt
     1561 cgttacgccg cgacagcgga gacacctcga caggatacgc gacaacgaac gtcggctggc
     1621 gcagcgcagc ctcacaggtc tgctcgaaga tctcgatctg cagcttgccg ctgccatagt
     1681 tctcttcgac ccgggcgccg actccttccg caaagtcctg caggtagtcg cggtcgcgaa
     1741 tcctggccgc atcgataccg ggattgaatt cgacgatcgc ctcttcgacc gtcagccgcc
     1801 tgaacgcctt cccgagatcg tattgctcgc cctggtagtc gaccacggcg gtgccgagga
     1861 ccgcctggga cattcccgtc agcagtgcct ccaccatttc catcaggtcg tggtagtcgg
     1921 cgtacgcccg gtacagttcc agcatcgtga attcaggatt gtgctgggtt gaaacaccct
     1981 cgttgcgaaa actgcgattg atttcgtaga cgcgttccag cccgccgacg atcaggcgct
     2041 tcaggtaaag ctccggcgcg atgcgcaggt agagctgcat gtcgagtgcg ttgtgatgcg
     2101 tcgtaaaggg ccttgcaacg gcacccccgg cgagggtctg catcatcggg gtttcaactt
     2161 cgaggaagtc ctgggactcc atgaaagcgc ggatgtaggc tacgatacgg gcgcgaatgc
     2221 gaaaaacgtg acgactcttc tcattcatga tcaagtcaac gtagcgctgc cgatagcggg
     2281 tttccatgtc cgcaagacca tgccatttct caggcagcgg tcgcagtgat ttcgtcagca
     2341 ggcgcacgtt gtccgccttc accgtcagct cgcccgtctt ggttttgaag agaagtccct
     2401 cggctccgac gatgtcgccg atgtcccact tcctgaaatc ctggtagagg ccttcgggca
     2461 gccggtcgcg ctcgaggaac acctggatct gcccggtacg gtcctgcaac ttcacgaagc
     2521 tcgccttgcc catgacgcgt ttcgccatca tccggccggc cacccgcacg cgcggcgaca
     2581 cttcaggcag cttgtccgcg tcgtggggtc cgaacgtaca atgaagttct tccgccaacg
     2641 cgttgcggcg aaagtcgttg gggtaggctt cgcccgccgc ccgcagctca ttgagctttc
     2701 gacgccgttc ggcgatgagg gcgttttcgt ccgcgtgctt tacgtcgtca ttcctcactt
     2761 acagcccctg cttcagactc gcctcgatga agcggtcgac gtcgccgtcg agcaccgcct
     2821 gcgtgttgcc tgtttcgacg ccggtacgaa gatcttttat ccggctctgg tcgaggacat
     2881 aggatcttat ctgacttccc cagccgacgt cggctttgct gtcctcgacc tcggccgctt
     2941 tcgcgcggcg ggtctgcagt tcgagctcgt acagctttgc cttcagttgt ttcatcgcag
     3001 tcgccttgtt cttgtgctgc gagcggtcgt tctggcattg cactacgatg ttggtcggga
     3061 gatgagtgat tcgcactgcc gactccgtac ggttcacgtg ctggccgccg gcgccgctcg
     3121 cgcggtacac gtcgatgcgg agatccgacg gatcgatctc gatttcgatc tcatcgtcaa
     3181 tttcgggaga tacgaagacg gaggcgaaag aggtatggcg gcggttgccg gagtcgaaag
     3241 gcgatttacg gaccagccga tgcacacccg tctccgtacg cagccagccg taggcaaact
     3301 cgccgacgac tcgcacggtc gcgctcttga tgcccgccac ttcgccggcc gaagcctcta
     3361 tgatttctgt ttcgaactcg tgcgcttccg cccagcgcag gtacatgcgc agcagcatct
     3421 cggcccagtc ctgggcttcg gtaccgccag cgcccgactg gatgtcgaga aaggcattgg
     3481 cgctgtccag ttctccgctg aacatgcgcc ggaattccag ttccgctatg cgctccgcat
     3541 agcctgagat ctccggcacg agggcatcga gtgtgctctt gtcgtcctcg tccagcgcga
     3601 gctcgaacaa ttcgcttgcg tctttaaggc cgcgcgacaa cttgtccagg ttgacgacga
     3661 tcttttccag cgcagcgcgc tcctgcccca gcgcctgggc cttttcgggt tcgttccaga
     3721 cgtccggctg ctccagctcg cgacgtactt ccgtcaggcg ttccgcgcgg ttctcgtagt
     3781 caaaggtacc tccttaacgc ttcggtgcgc tcggtgaggt ctgcgatctg ctgtttgatc
     3841 tgggtggttt ccatgtcgct ggctttccaa ggttctcttt cgcagcctgt gcctcaaacc
     3901 ggggccgcac cgaaaaacgc ggcatgttat cacgtcgtcg gtgacgccag cctgctgatt
     3961 tgttcgacaa cgagctgtgg cgactcgacg cctagccact cattgacgtc gagccggtac
     4021 gcgatcctgg cgctcccgcg gaaggccgca ctcgcctggt tgaaagcgat cgcgtcaatc
     4081 cgggcattgc catctagcgg cctcactttc agcttgaggt gcaactcgcc gacgattctt
     4141 tgttcgacca gttcaaaatc cccgcaccac agaggctccg ggaaacccgc accccagggg
     4201 ccgccgtcgc gcagaactct cgcgaaacgt aatgtcatcg atgtcgccgg caggtcgccg
     4261 tcgctaagga gggcaccgct gaaatcggcc tgtggataca ggcggtacaa ctgctggcaa
     4321 accaggactt tgaaatcctc gagatgcgcc gccggcaggc tcaagcccgc cgccattgca
     4381 tgcccgccga acctgggcac gagtcccggc cgaacggtgc agacggcttc cagcaggtcg
     4441 cgaatatgga tgccgggaac agagcgggcg gagcccttga gctgtccgtc ctggtcgcgc
     4501 gcgaacgcaa tgacgggacg atggcaacgc tcttttaccc gggatgcgat cagtcccacc
     4561 acgccctggt gccaggactc atcgtagacg cagacgcagc cgggcaatcc cgtctggtca
     4621 agacgatcga caaaggcaaa tgcctggtcg cgcattgacg tctcgatgtc acggcgctcg
     4681 tgattgatgc ggtcgagcgc ttccgcgtat cgggtggccg aagccgagtc gtcggtgaga
     4741 aggcattcga tcccgacgga catgtcgtcg agcctgccgg ccgcattgag gcgcggcgca
     4801 gcggcgaacc cgagatcggt gctgttcacc cgttcccgct gccggccgga taccgccagc
     4861 aatgccgaga taccggcagt gcaacttcct gcgcgtattc ttcgcaagcc ctgcatcacg
     4921 agtattctgt tgttctggtc gagccgcacg acgtccgcaa ccgtgccgag tgcgacgagg
     4981 tccaggtagc gtgccggtat ccttgctgct tcccgccgcc cctcgtgctg tagcgcattg
     5041 ccgagcgccg ccatggtata gaaggcgacg ccgactcccg ccaggttcgg actgccgaac
     5101 gtcgacccct taaggttcgg gttgacgatc gcggccgcgg caggcagctc tgctcccggc
     5161 aggtgatgat ccgtgatgac gacgtcgatc ccgagccggc gtgcttcggc aacgccttcg
     5221 atgctggaga tgccgttgtc cacggtcagc agcagcgccg gggacatcgc ggcagccaca
     5281 cgaacgatct ccggcgtcaa tccatatccg tactcgaagc gattcggcac gaggtaactc
     5341 acttccgcga agccgaagtc ccgcaggcag cggacgacga gtgccgtgct ggtcgcgcca
     5401 tcgacatcga aatcgccaat cacgatgatg cggcgccggc ggctcctcat gagcactgct
     5461 accgcagtgt ccacgccatc cagggtgcgc accggcacca ggcgttgcag ggtgagatcg
     5521 agctcggctt ccttcgcgat gccgcgagta acgtagagtc ttgcgagaat gggatcgagg
     5581 ctgctgaacg ctgcgacggc cgccgcgtcg ggtgattccc tgcgacgaac cggcctctct
     5641 ttggtcaccg tcatccgcgg ttgttttcct taaaatttgc gacgccacag ccgcagcgcg
     5701 tcgaagcggc caatatctac ccgcgacccc cggccgataa gcaacgtcac ccggcgcagt
     5761 ctgccacgtc gcagcatgcg gcgcagaaaa gcgagatgca catcgtcctg ctcactcctt
     5821 tccgcggccg cgggcgccgg caggacggcg acaaaaccgt ccggcgaacg cgagaggcac
     5881 gattcaaggt ctcccgtcca gtccgccacc gcggcagagg cggcgagcca gtagcccctg
     5941 catagcggct cgtttgcgta cagaggcggc aacggcatca tgggcagggc gggtgcgatg
     6001 ccgccgcccc acatccacaa cgatgtaacc ggcggttttc ccgcgatggc acgacgccgg
     6061 ttgacggcgg agtcgtacag gcacatctgc acctcgctct gcagcctgtc gtgtgctttg
     6121 gcctgctcgc cagtcggcaa gaagtcttca ggaaaattac cctgcgcgac ggcaggcgac
     6181 gcgtgcgcga ctgccatcgg gcgtcggcga tgaacatagc cgaatttgcc gacgcttgaa
     6241 aaaccctggt ccccgttgcc tgccaggttt tcctgcagat gagcgaatat gtcggctacc
     6301 tccgcaacat cgagatccgc ttcgtcgagc ttgtgcaaga cgacgtggtt gaggcgcgct
     6361 tcgagaaata ccgggtccgc cgcagcaacc catccttcgg gatgtgctcc ggtctggccc
     6421 cacaggcgca aagctcccaa accctccggc ggcgtcggcg cagcaaggat cgagaggatt
     6481 tgcgacagga tttcgccgga ggcggaactc acgcgcgagc gcgccagcca gcggtggctc
     6541 cgcaacggca cacgcgggga ttcgccctca tggggggccg gcggcaggac gaggatggcg
     6601 tgcttcttca ggggcatgtg cgggagggct ccttccgctt gtcctgcttc taatgatgct
     6661 tctgtgcact gttgcgggcg tattcttgat gtccgcggtt gaactgcaac cggatctttg
     6721 tatcatccgg tcagtagttc tgcgcgatgc aaacccttct ccgcgaactt tgacacgtga
     6781 cttgcggcag cgagccgcgg agccggccga tgcagtttac ccgcgaacag atatccgaga
     6841 tcacggtgcg gcaggtagag ccgggacgca tccgcatcgg taacgccgtc ttccataaca
     6901 acgtgctgct gaccgtggag cgcgcgatcc gtgactggag cctcgaagat atcgacgacc
     6961 tcgatgaaag cgcgctcgaa gccatcctcg aggatgcccc cgagatgatc gtggtgggca
     7021 cgggctggaa acccgtgttc ccgtcacgag aactggtgtt cgccctggcg cgccgcggca
     7081 tcggcttcga gacgatggat actccggccg cctgccggac gttcaacatc ctcatcaacg
     7141 aaggtcgccg cgccgccgcc gcgctgatcg tcaacggtcc agttccagcc ggggcttcgc
     7201 tcgccgccaa cgtagaccgt tcatgaacaa tgcgcgccgc gctgaagacg gacgcaccct
     7261 cagtcgagca aggcggctcc gttgaagccg tggctttcca tcatgcgttt gaggttgttc
     7321 agcgcgtcga gctggatctg gcgaacccgc tcccgggtga ccccgatctc ccggccgatt
     7381 tcctccagcg taaggcgctt gtgcccgtgc aggccgtaac gctgttcgac gaccgccctc
     7441 tgcttgctgt ccagtttcaa aagccaggcg tcgaggatag ctgtcacccg ttcctgctgg
     7501 acgcactgcg gcggttcggc ttcgggcccg tcagccagca tgtcgagcgg ggtggtgtct
     7561 tcgtcgctgg cggcagagcc gatgctgaca cgttcgtgaa gagcgatgag ccgttcgacg
     7621 tcggaaggct cgcgcttcaa gtgctgcgcg atctcggctg cggtgggagc cgcaccgttg
     7681 acctggcgca ggtagcgcgc agctcgcagg caggaattga tgtccttgat aacgtggatg
     7741 ggtaatcgaa ccgtacgcgt ctggttcatg atcgcgcgtt cgatcgtctg ccggatccac
     7801 caggttgcat aggtggaaaa gcgaaagccg cgttcgggat cgaacttgcg gacggcgtga
     7861 atcagtccga gattgccttc ttcgatcagg tcgagcagcg gcaatccccg atcgatgtaa
     7921 cggcgggcga ccttcacgac gagtcgcagg ttgctttcga tcatccgccg ccatgctgct
     7981 tcgtcgccct ggcgaatgcg ccgcgcgatc tggacctcct cctcggccga cagcaaccgt
     8041 gaagtcccga cctcgttcag gtagagacgc ttggcgtcgt gcggagaaac cgcactcgca
     8101 tcgacgcgta ttgattcttt cgaccagcct gcgactgcca ctgtagaaat cctcgctatt
     8161 gcaggcgagg gagtcgcggc atgtgaaccg gtctgtcgca tggaactgtg gctttggtta
     8221 agctgccatc atgaagagag cgcataccac agccgccagt gcgaagttgg cgctgatgta
     8281 aagtcgatta tctcgctggc agatattgtc gcgggtcaac cggcttgccg ttgcgcctga
     8341 tttcgaaatg cagtcgcggc tcacgtcccg gcccctcacc catcgttgct atgcgctggc
     8401 ccttcctgat gccttcgcct tcccgaacca gcagcgacgc gttgtgcccg tatgcactga
     8461 ggtaagtgtc gttgtgcttg atgataataa gctgcccata gccgatcagt ccaccgccgg
     8521 aatacaccac ccggccggcg gccgctgctt cgatcggctg gcccggtttg ccgtcgatca
     8581 ggatgccggt gccgctgccc gggcggccgc cgaattccac gaccaccctg ccgcttgtcg
     8641 gccattccca tggcggtgcc ggctcggcgg gaatcggtgg cagtggttct gcgcgcctcg
     8701 gcgccggccg gctgacgggc tgccgcggcg cggctgcacg ggcgccggca ggtggatcca
     8761 gctggatcac ctggccggga tagatcaggg agccgtcccc gagccggttc catcgtgcga
     8821 gatcccggta gtccctgccg taacgccagg ctatggtgaa cagcgtttcg ccggagcgca
     8881 cgatatgagt gtcgggtgag tggtcgcgac gcgtcgcagg tccacaggca gcggcaagga
     8941 gcgccgccag cagcagggac agccgaaaag gcatatgcat tccggcaccc gccgcgccgc
     9001 agtgcctcct ctctcgtctc attccttcat cattagaaag gcgacgccca ccactgcgat
     9061 cgctaccacg gcccagccga tgcgttccac gtacctcggc aacagggcag ccatgcgttc
     9121 accacctgcc acgatgaatc cggccacgag gtagaagcgc ccaccgcggc cgaggaagga
     9181 cgcgacgaca aaccacggaa aggtcagcac cgctgcgccc gctgctatag taaaaatctt
     9241 atacgggatg ggcgagaaac cggcgacgaa cacggccagt acgccccact tgtcaaacca
     9301 ggcccgcgcc gcgacatagc tttcccagta gtgagtcgtt tgcagccacg gctcgatcat
     9361 ctcgaacagg aagaacccga ttgcgtagcc ggcgacaccg cccagaaccg aggcaaccgt
     9421 ggtgacggta gcgtagcgcc aggcgcgttt tcgttcggcc aggcacatcg gcgccagcat
     9481 cacgtccggc ggtatcggga aaaaagaaga ttccgcgaag gaaaggccgg cgaggtaccg
     9541 ttccgcgtgg cggtgccgcg accattgcag tacgcgctcg tacagggggc cgaagaggcg
     9601 cattcagtac aggctccggc gcgcctggtg aaattg
//
LOCUS       DASUOM010000090         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_60873,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000090 DASUOM010000000
VERSION     DASUOM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60873"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFY27_01875"
     CDS             <1..708
                     /locus_tag="VFY27_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5764288.1"
                     /translation="DAGGPVEGVVISGVTPGSAASEAGLRSGDIITAINGESFNADDP
                     GQANQKLLDFMTAVEEGDTLDVEYLRNGQTASVEVKPRAMSDHAFAFRSPDFRGFAVP
                     GVPAAPAAPDARFFNFYAGSGSWGDMELVSLTEDLGRYFGTKDGLLIVRAPVDENLKL
                     RDGDVIQRIDGREPKSVSHAMRILGSYQGGETLEIEIMRDKKRQTLEVEMPDNRQSEV
                     REFGFRVPAAAAEPAEP"
     gene            780..1847
                     /gene="queA"
                     /locus_tag="VFY27_01880"
     CDS             780..1847
                     /gene="queA"
                     /locus_tag="VFY27_01880"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5764289.1"
                     /translation="MQAADFDYELPPELIAKYPAPERRGSRLMIVGPASDELHDRRFE
                     DLPDFLRPGDLLVFNDTRVMNARLCGQKASGGRVEILLERILSPHKATAKIRASKSPG
                     PGSHIHLQGGIAVEVTGRDGEFFRLEFSQPVAEVLDAIGEVPLPPYLERPAEPVDRER
                     YQTVYARERGAVAAPTAGLHFDEALLQQTLDAGVHHAFVTLHVGAGTFQNLRQEEILA
                     NRLHAERVRVPPETCEAIARTRSGGRRVFAIGTTSVRALESASAGGELRPFDGETDMF
                     ILPGYRFRSVDALLTNFHLPRSSLLMLVAAFAGTERILSAYRHAVAERYRFFSYGDAM
                     LVLPPRHAPHGPRSATHATNP"
     gene            1865..>2324
                     /locus_tag="VFY27_01885"
     CDS             1865..>2324
                     /locus_tag="VFY27_01885"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5764290.1"
                     /translation="MRFSITTNCGAARTGKLRFRRGEVRTPAFMPVGTYGTVKSVTPE
                     EVAGTGADIILANTFHLMLRPGPATIRRHGGLHEFMNWAGPILTDSGGFQVFSLAAMR
                     KLDPEGVRFRSPFDGAEVFLTPGRSIEVQHDLNSDVAMVFDECTPYPATHD"
BASE COUNT          425 a          730 c          751 g          418 t
ORIGIN      
        1 gacgccggcg gtcccgtgga gggcgtggtt atcagcggcg tcactcccgg tagtgccgca
       61 tcggaggccg gccttcgctc cggtgacatt atcacggcga tcaacggcga gtcgttcaat
      121 gcggacgacc cggggcaggc gaaccagaaa ctgctcgatt tcatgactgc cgtggaagag
      181 ggggacacgc ttgacgtgga atacctgcgt aacgggcaaa ccgcatccgt ggaggtcaag
      241 ccgcgggcga tgtcagacca tgcgttcgcg ttccgctcgc cggatttccg gggctttgcc
      301 gttcccggcg tgccggctgc gcctgccgcg cccgatgcgc gcttcttcaa cttctacgcg
      361 ggaagcggaa gctgggggga catggagctc gtgtcgctga ccgaggacct tggccgctac
      421 ttcggcacga aagacggact cctgattgtg cgcgcgccgg tggatgaaaa cctcaagttg
      481 cgagacggcg acgtcatcca gcgcatcgac ggaagggagc cgaagtcggt gtcgcacgcc
      541 atgcgcatcc tgggctcgta ccagggcggc gagactctgg aaatcgagat catgcgcgat
      601 aagaagcggc agacactgga ggtcgaaatg ccggataacc ggcaaagtga agtacgggag
      661 ttcggtttca gggtgccggc tgcggcagct gaaccggctg aaccctgatc gggcaccgcc
      721 agcataatcg cgcaccaggg gctgtcaggg cctgttaaca ggccctaata tgcgcgccca
      781 tgcaggcagc ggatttcgac tatgagctcc caccggaact gattgcaaag tacccggctc
      841 cggagcgccg gggcagcagg ctgatgatcg tcggtccggc cagtgacgag ctgcacgacc
      901 ggcgattcga agacctgccg gacttccttc gtcccggcga cctgctggtg ttcaacgata
      961 cgcgcgtgat gaacgcgcgg ctctgcgggc aaaaagcgag cgggggccgg gtggagatac
     1021 tgctcgaacg gatactctcg ccgcacaaag cgaccgcgaa aatccgggcc agcaagagcc
     1081 caggacccgg atcgcacatc cacctgcagg ggggcatcgc ggtcgaagtg accggccggg
     1141 acggcgagtt cttccgcctc gagttttcgc aacccgtcgc ggaagtgctg gacgctatcg
     1201 gtgaggtccc gctgccgccg tacctcgagc gacctgcgga acctgtcgat cgtgaacgtt
     1261 accagacggt ttatgctcgc gaacgcggcg ccgtcgcggc gcccactgcc ggactgcact
     1321 tcgacgaggc actgctgcaa cagacgctcg atgcgggcgt gcatcacgcc ttcgtgacgc
     1381 tccacgtggg tgcaggaacg ttccagaatc tgcggcagga agaaatcctc gcgaatcgcc
     1441 tgcatgcgga gcgcgtgcgg gtgccgcccg aaacctgcga ggccatcgcg cgtacgagaa
     1501 gcggcggccg cagggttttc gccatcggta cgacatctgt ccgcgcgctg gaatccgcgt
     1561 ccgcgggcgg tgagctgcga ccgttcgacg gcgagacgga catgttcatc ctgcccgggt
     1621 acagatttcg ttcagtggat gcgctattga cgaattttca cctcccgcgt tcttcgctgc
     1681 tgatgctcgt tgcggcgttc gccgggaccg aacgtatact gtctgcctac cgccatgcgg
     1741 tcgctgaacg ctatcgcttt ttcagttatg gcgatgcaat gctggtactg ccgcctcgac
     1801 acgcacctca tgggccgcgc tccgcgactc atgcaacgaa tccctgaccc aggaccacca
     1861 gccgttgaga ttttccatca ccacgaactg cggcgccgcc cgcacgggca agctccgctt
     1921 ccgtcgcggc gaagtgcgca cacctgcttt catgccggtc ggaacctacg gcaccgtcaa
     1981 gagcgtcacg cccgaggagg ttgcgggaac gggtgccgac atcattctcg ccaatacctt
     2041 tcacctgatg ttgcgccccg ggccggcaac catcaggcgc cacggcggcc tgcatgagtt
     2101 catgaactgg gctggcccca tattgacgga ctccggcgga ttccaggtgt tttcgctcgc
     2161 tgccatgcgc aagcttgacc ctgagggcgt acgctttcgt tctccgttcg acggcgccga
     2221 agtattcctc acgccggggc gttcgatcga ggtccagcac gacctgaact cggatgtggc
     2281 gatggtattc gacgagtgca ctccgtatcc ggcgacgcat gacg
//
LOCUS       DASUOM010000091         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1726095,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000091 DASUOM010000000
VERSION     DASUOM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726095"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /gene="bamA"
                     /locus_tag="VFY27_01890"
     CDS             complement(<1..2028)
                     /gene="bamA"
                     /locus_tag="VFY27_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5764291.1"
                     /translation="MRSWFASALALTASAGAIIVAQDLTYLSRAWAQQPGQVISRILV
                     EGNQRIEARTVLSYLLVRPGDAYDEERIGLSVRALFATGLFSDVQFELRGSDLIVRVI
                     ENPIINRVIFEGNSAVTTERLEEETEAEPRQTFTLSRVEQDVQRILEVYRRAGRFAAT
                     VTPQYKVLPQNRADLIFVIEEGPTTGIRSINFLGNERFSDDRLRREITTRQSRWWRFF
                     ERADNYNPGQIEFDRTQLSEFYMNQGYADFRVISSVAELTPDRRDFYVTFTVEEGEPY
                     TFGDLSVETELEKLSGERLSLILPIKKGDPFASKSIESAIDTLTFAAGTAGYANVDIR
                     PVERRDPVNHTISYTFQVDEGPRVYIERIDIEGNTQTLDRVIRREIRAAEGDSYNPVL
                     LDNARNRIRALGFFNPDTVEVTDEPGSTDDRTVLKVRVEEEATGELAFSAGFSSEDAF
                     LFSVSAQQRNFRGRGQFLSARIQATSRQQDIEFRFTEPKFQDRNLAVGFDLFATQSDF
                     LDYAGFENSVVGAGGRLLFPLSDTDQIGLRYNLRQDDLTVDERLLGSGGIGDCSAGSF
                     FRSSICDQQGSRLTSAFGYTLTMNRTNDYLEPTRGYEVNFSQDIAGLGGDVNYLRNEL
                     RAATYRGLWPGIVASAKLEAGFIDSWGDEGVRINNRFFKGGNSFRGFDVAGL"
BASE COUNT          446 a          664 c          658 g          423 t
ORIGIN      
        1 taagcccgca acatcgaacc cgcggaagga attgccaccc ttaaagaacc ggttgttgat
       61 gcggacgcct tcgtcgcccc agctatcgat gaacccggcc tcgagtttgg cactggcgac
      121 gatgcctggc cacaggccac ggtaggttgc tgcgcggagt tcattccgaa ggtagttcac
      181 atctccgcct agaccggcaa tgtcctggct gaagttgacc tcatatccgc gcgtgggttc
      241 cagatagtca ttggtacgat tcatagtcag cgtatatccg aacgccgatg tcagccggct
      301 gccttgctga tcgcagattg aggagcggaa gaaggagcct gccgagcagt cgccgatgcc
      361 tccgctgccc agcagtctct catcgaccgt cagatcatct tgccgcagat tgtagcgcaa
      421 gccgatctgg tcggtatctg acagcgggaa cagcaggcgc ccgcctgcgc cgacgacgga
      481 gttctcgaac cccgcatagt caagaaaatc ggactgcgtc gcgaaaaggt cgaaaccgac
      541 agcaaggttg cggtcctgaa actttggctc ggtaaaacgg aactcgatgt cttgctggcg
      601 cgatgtagct tgtatgcgcg ccgacaggaa ctggccacgg ccgcgaaaat tgcgttgctg
      661 cgcgctgact gaaaagagga aggcatcctc ggaggaaaag cctgccgaga aggcgagttc
      721 gcccgttgcc tcttcttcga ccctaacctt gagtacagtg cggtcatcgg tcgagcccgg
      781 ttcgtccgtc acttcgacag tgtccggatt gaagaagccg agagcgcgga tgcgattccg
      841 ggcattgtcg agcagcaccg gattgtacga gtcgccctcg gcggcacgta tctcacggcg
      901 gataacacga tccagtgttt gcgtatttcc ttcgatatcg atgcgttcga tatagacccg
      961 cggaccctcg tcgacttgga acgtgtagct gattgtatga ttcacggggt cgcggcgctc
     1021 gacgggccgg atgtccacat tggcgtatcc ggcggtgccg gccgcgaacg tcagcgtgtc
     1081 gatagccgac tcgatgctct tggaggcgaa cgggtccccc ttcttgatcg gaaggatcag
     1141 cgacagccgt tcgcccgaca gcttctcaag ctccgtctcg actgaaagat caccgaacgt
     1201 gtagggctcc ccttcctcga cggtgaacgt gacgtagaag tcccgccgat ccggagtgag
     1261 ttcggcaacc gaggagatca cgcggaaatc cgcgtacccc tggttcatgt aaaactcact
     1321 gagctgagtg cggtcgaatt cgatctggcc ggggttgtag ttgtccgctc gctcgaagaa
     1381 gcgccaccag cgggattggc gcgttgtgat ctcgcgacga aggcgatcgt ccgaaaagcg
     1441 ctcgttgccc aggaagttga tggagcgaat gccggtggtg ggcccttcct cgattacgaa
     1501 gatgagatcg gcccggtttt gcggcagcac cttgtactgc ggcgtcaccg tcgccgcgaa
     1561 acgtcctgcc cggcgataga cttcaaggat gcgctggaca tcctgctcga cgcgcgagag
     1621 ggtaaaggtc tggcgcggct cggcctcggt ctcctcctcc agccgctcag tcgtaacggc
     1681 gctgttgccc tcgaaaatca cgcgattgat gatcgggttc tctatcacgc gcacaatcag
     1741 gtcgctgccc cgcagttcga actgaacatc gctgaacaag cccgtcgcga acagcgccct
     1801 gaccgagagg ccgatacgct cctcgtcata cgcgtcgccc ggcctgacca gcaaatacga
     1861 caggacggtg cgcgcctcga tacgctggtt gccctctacc aggatccgag aaatgacctg
     1921 ccccggctgt tgcgcccacg cccgcgagag gtacgtcagg tcctgcgcga cgatgatggc
     1981 gccagccgac gccgtcagcg ccagagcgct cgcgaaccaa gaccgcatcg cctacccctc
     2041 actcatcgga ccgcctgacg cgatctcttc gggcgtctta agacaacatg ccgctgaaaa
     2101 gctcaagcgg ctgaatggtt tgctcagggc gccccggacc acaggggcag gcgagtaatg
     2161 tcctgccagg tgatcacaag agcaaagctg a
//
LOCUS       DASUOM010000092         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1483034,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000092 DASUOM010000000
VERSION     DASUOM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483034"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFY27_01895"
     CDS             <1..608
                     /locus_tag="VFY27_01895"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019589282.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX5764292.1"
                     /translation="IVGGANVNFDRLRHIAERAAVGEQREMLLAVEIPEQPGSFRKFC
                     EAIGRRSVTEFNYRYSDSAKAHIFVGIELRHGQAERTRLINKLGALGYRIQDLSDNEL
                     AKLHIRHMVGGGAMPENEKLFRFEFPERPGALLDFLNAIGSNWNISLFHYRNHGSDHG
                     RILAGIQVPPDEVDELETHLAELKYPHWDESGNPAYRMFLA"
     gene            complement(991..1647)
                     /locus_tag="VFY27_01900"
     CDS             complement(991..1647)
                     /locus_tag="VFY27_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764293.1"
                     /translation="MISNLQKHLTDIYQVDPGFEVTDFLITDPTLARLLGDGSLVPNT
                     DESVLLSQDENGLALSLYLEEEMVERLTRDNPMDELRADRLDDLCKVIEGISHFNYIV
                     WRARQDKAVTLLELEMQAEVDKFVGTLLMALEQEDFEFARRLHGWLFDRVSFHPDLNA
                     DQLERYRAANDFAARFCHGLRDRLERDSTRGMHELRRFYRLSQAEKISHIRTQAYQRS
                     "
     gene            complement(1647..2279)
                     /locus_tag="VFY27_01905"
     CDS             complement(1647..2279)
                     /locus_tag="VFY27_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764294.1"
                     /translation="MPHRETAEYVVPVQNLRDYFRESMDAAIDKQGVDVDPHATHYVV
                     NLLTLFARSEDLYEDHGPAYGLKPLALMMVDAVDAPTAEQRNFALQRIGDVSLFIAGF
                     FADSLAHRLVDLDYYINMGGNAYGSLSDEMRGTLRGKALANVYRELAHKFQTVVDILN
                     EVRDGARQSTDIDLLRTYEVWLRTGSKRAESLLRQNGVVPMPNMTSRRSH"
BASE COUNT          509 a          710 c          706 g          496 t
ORIGIN      
        1 ccatcgtcgg cggcgccaac gtcaacttcg accgcctgcg ccacatcgcc gaacgcgccg
       61 cggtgggcga acagcgggag atgctgctgg cagtcgagat cccggaacaa cccggtagct
      121 tccggaagtt ctgcgaagcg atcgggcggc gcagcgtcac cgagttcaac tatcgctaca
      181 gcgattccgc caaagcgcac atcttcgtcg gcatcgaact gcggcatggg caggcggaac
      241 ggacaaggct cataaacaag ctgggagcac tcggctaccg gatccaggac ctgagcgaca
      301 acgaactcgc gaaactgcac attcgccata tggtgggcgg cggtgccatg cccgagaatg
      361 aaaaactgtt ccgcttcgag tttccggagc ggccgggcgc gttgctggat ttcctgaacg
      421 cgatcggcag caactggaac atcagcctgt tccactaccg caatcacggc tcggaccatg
      481 gccgcatact cgccggcatc caggtgccgc ccgacgaagt cgatgaactc gagacgcacc
      541 tggcggagct caagtatcca cactgggacg aaagcgggaa cccggcctac aggatgttcc
      601 tggcttaggt gctgcaggca gcggggcggc cacctgcagg aaagccgtta ccgctgaaac
      661 tttgccgctg tttcatgaat tgcctgtcgc ggcgtttttc tgcgttgctt attccaaact
      721 gttcgcaacc ctttccttcc ggggtgcatc aaaccatcaa ctgtaacgag aaaagatcgc
      781 gcgacggctg tccagcgcga gtcgaaaagg gaagggcaac gatcacgaac atagagttct
      841 ccggtaccgg gttcatgcag tcatgcaccg gcgacaggga cctgaaggta tcggtgaagt
      901 aaacgtaaca aacttatccc ttatgcagtt tgtttcgtaa gcttggacgg tggctatgcc
      961 accgtccttt ttttatggcc tggaccaggt ttagctgcgc tggtacgcct gcgtgcggat
     1021 gtgactgatt ttctccgcct gagacaggcg atagaaacgg cggagttcgt gcatgcccct
     1081 ggtactgtcc cgctcgaggc gatcgcgcag gccgtgacag aagcgtgccg cgaaatcatt
     1141 ggctgcacgg tagcgctcga gctggtccgc attcagatcc ggatgaaaac tgacccggtc
     1201 gaacagccat ccgtgcagcc ggcgcgcgaa ctcgaaatcc tcttgctcca gggccatcag
     1261 tagcgtgccg acaaacttgt ccacctcggc ctgcatttcc agttccagga gcgtgaccgc
     1321 cttgtcctgc cgtgcacgcc atacgatgta gttgaagtga ctgatgcctt cgatgacctt
     1381 gcagagatcg tcgagccggt ccgcccggag ctcgtccatg gggttgtcgc gagtcagcct
     1441 ctcaaccatc tcctcttcaa gataaagact cagcgccagg ccgttttcat cctggctcaa
     1501 tagcacggat tcatcggtgt tcggaacgag cgagccatcg ccgagcagtc ttgcgagcgt
     1561 cgggtcggtg atcaggaagt cggtcacctc gaagcccgga tcgacctggt aaatatccgt
     1621 caggtgcttt tgaaggtttg aaatcatcag tgacttctgc ggctggtcat gttcggcatc
     1681 ggcactacgc cgttctgccg caacagtgat tcggcacgct tgctgccggt gcgcagccat
     1741 acctcgtagg tccgcaggag gtcgatgtcg gtcgactgcc gcgcgccgtc gcgaacctcg
     1801 ttcaggatat cgaccaccgt ctggaattta tgcgccagct cgcggtagac gttggcgagg
     1861 gccttgcccc gcaaggtgcc gcgcatctcg tccgacaggg aaccgtaggc attgcctccc
     1921 atgttgatgt agtaatcgag atcgacaagc cggtgggcaa ggctgtcggc gaagaagccc
     1981 gcgataaaaa gtgaaacatc gccaatgcgc tgcagcgcga aattacgttg ctcggcggtt
     2041 ggcgcatcga cagcgtccac catcatcagc gcgaggggct tgagcccgta agcgggaccg
     2101 tgatcttcgt acaggtcttc cgatcgtgcg aatagcgtca aaaggtttac gacgtaatgc
     2161 gtcgcgtggg gatcaacgtc tacgccctgc ttgtcgatcg cagcgtccat ggattcgcga
     2221 aagtaatcgc gaaggttctg taccggtaca acatactcag cagtttcacg atgtggcatt
     2281 gtctcgcttc ctcactctcg tcaggataga cggcacactc gttgcagccg gcaagcaaaa
     2341 aatgcagtgc tgagtccttt cagcagcgcg tcgactcgtc ggacattgag tacaccgcac
     2401 tacagcgcct tcagtcaatt c
//
LOCUS       DASUOM010000093         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_396267,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000093 DASUOM010000000
VERSION     DASUOM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396267"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFY27_01910"
     CDS             <1..467
                     /locus_tag="VFY27_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764295.1"
                     /translation="QVPRNTYLVGTFGVTGSLFLILYVTFLGSQEPFYEFMRRYGVYM
                     YFLLNVIAQIVLATKVLPVAKALGMPRLVTLTKVQLVLAWFPFALGALNLVLKATLED
                     AGPAESRIEWIFAMQMQVYFLVSYLSWKETGFRAAFDAGSGASASLASPQRS"
     gene            complement(479..1606)
                     /locus_tag="VFY27_01915"
     CDS             complement(479..1606)
                     /locus_tag="VFY27_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HEX5764296.1"
                     /translation="MRITVGLMIAAAVAAAAFSGLVNRDYRWNLPEGVPLPAVPSDNP
                     MSDAKVDLGRHLFYERRLSVTGATSCATCHMQALAFTDGRARSVGATGEVHPRGSMTL
                     VNAAYAGRLTWANHLLDTLEVQALTPMFGEQPVEMGMSGREDEIIQALRYDEYYAKAF
                     RASFPGDADPLSTLNVVRALASFVRTIVSFDSPYDRYLRGDTEALKPEQVRGMDLFFS
                     ERLECFHCHGGFNFTDSSTHAGSLVQSVGFHNNGLYNIDASGAYPPENTGLYDLTGER
                     RDMGRFKAPTLRNIAATAPYMHDGSIETLSGVLDHYERGGRVINDGPYVGDGSRNPYR
                     SEFVRGFELTAQERADLLAFLESLTDDSVPGNPAWSDPFDD"
     gene            complement(1612..2583)
                     /locus_tag="VFY27_01920"
     CDS             complement(1612..2583)
                     /locus_tag="VFY27_01920"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family copper-binding protein"
                     /protein_id="HEX5764297.1"
                     /translation="MRAKAIDPTNSRSPLRMTTRWESTQLPSPRMPKPFRRGCCFEYT
                     AVHTAFVQEFSRFLRAMLARHLILLFMISACSRPQQAIEIPFEPRFGEQPISCGADAT
                     GIAMTDLRFYVHDLRLVSTDGTDTPATLLEDPLWQSNAVALLDFENGAGACINGTQQT
                     NSVIRAQVPEGAYSGIRFRIGVPEDLNHADPLRAEAPLNYSVMHWHWRTGYKFLRAGV
                     TNVTDGFWMHLGSSRCEGTSSNVSSCIAPNRPLVELTAFVPGEDAVEVDLQQLVREID
                     LADGSPTDCSSGPAESECEMPFQALGLDFATGEAGGAASIFRKRSRE"
     gene            complement(2613..>3016)
                     /locus_tag="VFY27_01925"
     CDS             complement(2613..>3016)
                     /locus_tag="VFY27_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764298.1"
                     /translation="PDPRDTRIRSLEADVRVAQTAAEKVKTRLEEMNAELSVVRKALQ
                     EREEELEKQRTTTSVLRTDLKDSVKKTRELREELAQRATENVRSEVKLREVETELSLV
                     HASTDLIATGMLDYVTAEGDDEDVRVSKARG"
BASE COUNT          564 a          922 c          917 g          613 t
ORIGIN      
        1 gccaggtccc gcgcaacacc taccttgtcg gtacattcgg tgtcaccggc tcgctcttcc
       61 tgatcctgta cgtgacgttt ctcggcagcc aggaaccgtt ttacgaattc atgcgtcgct
      121 acggcgtgta catgtacttc ctgttgaacg tcatcgccca gatcgtactg gcgaccaagg
      181 ttcttcctgt tgccaaagcg ctcggcatgc cgcggctggt aacgctgacg aaagtgcaac
      241 tggtgctggc atggtttccg ttcgcgctcg gcgcgctcaa cctggttctc aaagcgacgc
      301 tagaggatgc cggtcctgct gaaagccgca tcgaatggat ctttgccatg caaatgcagg
      361 tctacttcct cgtcagctat ctctcctgga aagagacagg ctttcgcgcg gcgttcgacg
      421 ccgggagcgg cgccagcgcg tctcttgcat ctccgcagcg aagctgactg tttccaggtc
      481 agtcgtcgaa agggtctgac caggcaggat taccggggac gctgtcgtca gtgagcgact
      541 ccaggaaagc cagcaggtct gccctctcct gcgccgtcag ctcgaaacct cgaacaaatt
      601 cggagcggta cgggttacgg ctaccgtcgc ccacgtatgg accgtcattg atcacgcgcc
      661 cgccccgttc gtagtgatcg agcactccgg acagcgtctc gatgctgcca tcgtgcatgt
      721 acggcgcagt cgctgcgatg ttcctcaacg tcggcgcctt gaagcggccc atgtcccggc
      781 gctcgcccgt caggtcgtag agacccgtgt tctcaggcgg gtaggcgccc gaagcgtcga
      841 tgttgtacag cccgttgtta tgaaagccga cgctctggac cagcgagccg gcgtgagtgc
      901 tgctgtcggt aaagttgaag ccgccgtggc agtgaaagca ctccagccgc tcggaaaaga
      961 acaaatccat gccgcgaacc tgttcgggct tcagagcttc ggtatcaccg cgaagatagc
     1021 ggtcataggg agaatcgaag gatacgatcg tgcgcacgaa gcttgcgagg gcacgcacga
     1081 cgttgagtgt cgataaagga tctgcgtcac cggggaagga ggcgcgaaag gctttggcgt
     1141 agtactcgtc gtagcgcaag gcctggataa tttcatcttc gcgaccggac atgcccattt
     1201 cgaccggctg ctcgccgaac ataggcgtca gcgcctgtac ttcgagggta tcgagcagat
     1261 gattcgccca ggtcagcctg ccggcatacg ctgcgttgac cagcgtcatg gatccccgcg
     1321 gatgcacctc accggtagca cctaccgaac gtgctctccc atccgtgaag gcaagcgcct
     1381 gcatgtggca ggtcgcacag gaggttgcac cggtgacgga gagccgcctc tcatagaaca
     1441 ggtggcgccc gaggtcgacc ttcgcgtcgc tcatcgggtt gtcggacggc accgccggaa
     1501 gcggcacgcc ctccggcagg ttccaccgat agtcgcgatt gacgaggccg gaaaatgctg
     1561 ccgccgctac cgcggcagcg atcatcaggc cgacggttat tctcatcccg gtcattcgcg
     1621 agaccgcttg cggaatatcg aagctgcacc cccggcttcg cccgtcgcga aatccaggcc
     1681 aagcgcctgg aatggcatct cgcactcgga ctcagccggc ccagaggaac aatcagtggg
     1741 ggaaccatcg gcgaggtcga tctcccgcac cagttgctga agatccacct ccactgcgtc
     1801 ctctcccggc acgaaggcgg tgagctcgac cagcggccgg ttgggcgcaa tgcaactgct
     1861 gacgttgctg gaagtgcctt cgcagcggga gcttccgagg tgcatccaga aaccgtcggt
     1921 aacgtttgtg accccggccc gaaggaactt gtaccctgtt cgccagtgcc agtgcatcac
     1981 tgaataattg agcggtgcct ccgcgcggag ggggtcggca tggttcagat cctccggcac
     2041 accaatgcgg aaccggatgc cggaatatgc accctccggc acctgtgcgc ggatcacaga
     2101 gtttgtctgc tgtgtcccgt tgatgcacgc tcccgcaccg ttctcgaaat cgagcagcgc
     2161 caccgcattg ctctgccaca gcggatcctc gagtagcgtt gcgggcgtgt cagtcccgtc
     2221 agtactcacc agtcgcaggt cgtgtacgta aaacctgaga tccgtcattg cgattccggt
     2281 ggcgtcggcg ccgcaggata ttggctgttc accgaaccgc ggctcgaaag gaatctcaat
     2341 tgcctgttgg ggcctggagc acgcggaaat catgaatagc aggatgaggt gtcgggcaag
     2401 catggcgcga agaaagcgtg agaactcctg cacaaatgcc gtgtggactg cagtgtattc
     2461 gaagcagcaa ccgcggcgga atggtttagg catccgcggt gagggaagtt gcgtggattc
     2521 ccatctcgtg gtcattcgca gcggagaacg ggagttcgtt gggtctatcg ccttcgcccg
     2581 catggcgggc tcctacaccc ggtgggggcc agttagcccc gggccttgga tacccggacg
     2641 tcttcgtcgt cgccttccgc agtgacgtag tccagcatgc cggtcgcgat caagtcggtg
     2701 gaagcatgca ccagcgacag ctcggtctcc acttcccgca acttcacttc ggaccggacg
     2761 ttttcggtgg cgcgctgggc cagttcctcg cgcagctccc gggttttctt gacagaatcc
     2821 ttgaggtcgg tgcggagcac gctggtagtc gttcgctgtt tttcaagctc ctcctcacgt
     2881 tcctgcagcg cttttcgaac caccgaaagc tctgcattca tctcctcaag gcgagttttg
     2941 accttctccg cggcggtctg tgcgacccgc acatcggcct cgagcgagcg tattcgggtg
     3001 tcacgcggat cgggat
//
LOCUS       DASUOM010000094         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_880295,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000094 DASUOM010000000
VERSION     DASUOM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880295"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFY27_01930"
     CDS             <1..1238
                     /locus_tag="VFY27_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718787.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX5764299.1"
                     /translation="DERFVRRCNEWFARDIEDSGLRLPAGAPPMFATFRLRDMELENR
                     VIMSPMAQYSAVEGLPNDWHFVHYASRAIGGAGLIFTEMTCPSADGRITPGCTGLWSV
                     EQADGWRRIVDFVHGHTQAKFCMQIGHAGRKGSTRVAWEGMDQPLIHGNWPIVSASPI
                     PYRDGSQVPAELTEARMDEIVEQFAESARLADRAGFDMIEAHAAHGYLLASFLSPLTN
                     QRSDRYGGSVENRLRFPMRVVQRMREVWPADKPMSVRISASDWAPGGITDSDVDAIAL
                     AFKEAGIDIINVSTGQTVSHEKPVYGRMFQVPFADRIRQTVKIPTIVAGNITTADQVN
                     TILMAGRADLVALARPHLSDPYFMQRAMAWYGVQTEHACAPQYLSAMNQALRLSERQR
                     AEWLDTKRSLKPPSHEVTE"
     gene            1243..2703
                     /locus_tag="VFY27_01935"
     CDS             1243..2703
                     /locus_tag="VFY27_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764300.1"
                     /translation="MWRPAERIKYQPVPGPWPSREEAERSRLFSPVDVGPIRLLQRTW
                     VPAMVPWRATEDGDITPELLAWYRRFARGKPGAIVVEATGIRDIPSGPLLRIGHDRYL
                     SGLRKLVETVREASEGETRLFIQIIDFLQIRRRPTTEKFIRKYLVIGDHHRTAIGAEG
                     ASDDEVRAKLLTLDDDGLRNVLSEREHEACMMGHRERVGDLHLPHIRDLPRTLPPLFA
                     EAAARAREAGFDGVELHYAHAYTMASFLSALNQRDDGYGGDHAGRVRLPLEVYREVRR
                     QVGDDYVVGCRYLSTDCIEGGMTTGDAAQFGVEFARAGMAFLSLSRGGKFEDAKQPKV
                     GAAAYPYTGRSGYECIPQYISDKTGPFGRNLGATATIRGAIRAAGFDTPVVAAGGIHG
                     FAEAERVLADGEADIIGAARQSLADPDWFLKMRLGLGNDVRLCLYTNYCEALDTRHKQ
                     VTCELWDREGLDEDELMKSSDGRRRLNAPDWEPPPA"
     gene            complement(2727..2927)
                     /locus_tag="VFY27_01940"
     CDS             complement(2727..2927)
                     /locus_tag="VFY27_01940"
                     /inference="COORDINATES: protein motif:HMM:NF022385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX5764301.1"
                     /translation="MKQQDDKLPFEDLEKVYDLLAAAIDGAGPEKEALFLAKLCLTLA
                     HEIGDLTTVEQAIDIAKADLHP"
     gene            complement(2924..>3936)
                     /locus_tag="VFY27_01945"
     CDS             complement(2924..>3936)
                     /locus_tag="VFY27_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5764302.1"
                     /translation="RSFFGSRANPDSTILVHRQPDDIWRIDWQLQPHENPDQAIAEEN
                     VRSRVAAILDMLGHAGRWELEWWSIYTANTLCLDDYRHGRILFIGDAARIVPIFGVRG
                     LNNGFDDAMNAAWKLAYVLKGEAGEGLLESFTPERRGATLDIFSNAGKSSRFMTPPSR
                     GYALMREAVLQLSLTQEFTQKFADPRQVVPYTYGDSPLTSYRHRDSEFQGGVMAGSAA
                     VNRKAANGAFLLDYLGLGFTGLYFMDGENVPSDILDLSRRLAAIDHDFLLVLISRKKL
                     PEEMLTVIPDIEGCISDTYAATDGSFYLLRPDRHVAARWVALRVDEIEDALNISLGRV
                     TR"
BASE COUNT          792 a         1211 c         1220 g          713 t
ORIGIN      
        1 gggatgaacg gttcgtccgg cgctgcaatg agtggttcgc ccgggacatc gaggactccg
       61 gcctcagact accggccggc gcgccgccaa tgttcgcgac attccgcctt cgggacatgg
      121 aactcgagaa ccgcgtaatc atgtccccga tggcacagta ttccgcggta gaaggcctgc
      181 cgaatgactg gcatttcgtg cactacgcaa gccgtgccat cggcggcgcc ggcctgatct
      241 ttaccgagat gacctgcccg tccgccgatg gacgcatcac gccaggatgt accgggctgt
      301 ggtccgtgga acaggcagac ggctggcgaa ggatcgtcga cttcgtgcac ggccatacgc
      361 aggcaaagtt ctgcatgcag atcggtcatg cgggccgcaa gggctcgacg cgggtagcgt
      421 gggaaggaat ggaccagccg ctgatacacg gtaactggcc gatcgtgtcg gcttccccga
      481 ttccgtaccg tgacgggagc caggtaccag cggaactgac agaagcccgc atggacgaaa
      541 tcgtcgaaca gttcgcggag agcgcccgtc tcgccgacag ggccgggttc gacatgatcg
      601 aagcacatgc cgctcacggc tacctgctcg cgagtttcct gtctcccctc acgaatcaac
      661 gcagcgatcg ctatggcgga agcgttgaaa accggctgcg attcccgatg cgcgtcgtgc
      721 aacgaatgcg tgaagtctgg cccgccgaca agccgatgtc ggtccggata tccgcgagcg
      781 actgggcgcc cggcggcatc accgacagcg acgtcgatgc gatagcgctc gcgttcaagg
      841 aagcaggcat cgatatcatc aacgtatcga ccgggcagac ggtgagccat gaaaagccgg
      901 tctatggccg gatgttccag gtaccgttcg cggaccgaat ccgccaaacg gtgaaaattc
      961 ccactattgt cgccggcaac attaccaccg cggaccaggt caacacgatc ctgatggccg
     1021 gacgtgccga tctcgttgcc ctcgcccggc cgcacctctc ggacccgtac ttcatgcagc
     1081 gcgcgatggc ctggtatggc gtgcaaaccg agcacgcctg cgcgccgcaa tacctctcgg
     1141 caatgaacca ggcgctccgc ctgtcagagc gtcaacgggc ggaatggctg gatacgaaga
     1201 ggtccctgaa accgccctca catgaagtga cggagtaagg atatgtggcg gcccgcggaa
     1261 cgcattaagt accagcccgt accgggccca tggcccagcc gggaagaggc cgaacgatcg
     1321 aggctgttca gcccggtcga cgtaggaccg atccggttgc tgcaacgtac ctgggtgcct
     1381 gccatggtgc cctggcgggc gacggaagac ggcgatatca cgccggagct cctcgcctgg
     1441 taccggcggt tcgcgcgcgg caagcccggc gcaatcgtcg tcgaggcgac cgggatccgc
     1501 gacatcccga gcgggcccct gctgcgcata gggcacgacc gctatctttc aggactgcgc
     1561 aagctcgttg aaacggtccg tgaagccagc gagggcgaga cgcggctgtt catacagatc
     1621 atcgatttcc tgcagatccg ccgcaggccg acgacagaaa aattcatccg caaatacctg
     1681 gtgatcggcg atcaccatcg tacggcgatt ggcgcggagg gggcgagcga tgacgaggtg
     1741 cgcgcgaagc ttttgacact ggacgacgat ggcctccgca atgtgttgag cgaacgcgaa
     1801 cacgaggctt gcatgatggg ccatcgcgag cgcgtgggag acctccacct gccgcatatc
     1861 cgcgacctgc cgcgaacgct cccacctctt ttcgctgaag cggccgcgcg ggcgcgggaa
     1921 gccgggttcg acggtgtcga gctgcattac gcccatgcct acacgatggc atcgttcctg
     1981 tcggcactga accagcgcga cgacggctat ggcggcgacc atgccggacg cgtccggttg
     2041 ccgctcgagg tctatcgcga ggtccgcagg caggtagggg acgattacgt tgtcggttgc
     2101 cgatatctct cgaccgattg catcgagggc ggcatgacga caggagacgc cgcgcagttc
     2161 ggggtcgaat tcgcgcgcgc cggcatggct tttctctcgc tgtcacgcgg cggaaaattt
     2221 gaagatgcga agcagcctaa agtaggcgcg gccgcgtatc cctataccgg aagaagcggc
     2281 tacgaatgca taccgcaata catatcggac aagacgggtc ccttcgggcg aaacctcggc
     2341 gcgacggcca cgattcgtgg cgcgatacgt gccgccggat ttgacacgcc ggtcgttgcg
     2401 gccggcggca tccacgggtt cgccgaagcc gaacgggtac tggccgatgg cgaagccgac
     2461 atcatcggag ccgccaggca atcgcttgcg gatcccgact ggttcctgaa gatgcgcctg
     2521 gggctcggca acgatgtgcg gctgtgtctc tacacgaact attgcgaagc gctcgatacc
     2581 cgtcacaaac aggtgacgtg cgaattatgg gaccgcgagg ggctggatga agacgagctg
     2641 atgaagtcgt cggacggacg gcgacgcctg aacgctccgg attgggagcc tccgccagcc
     2701 tgaggccagc aggcattctt tcgatctcag ggatgcagat ccgccttggc aatatcgatc
     2761 gcctgttcga cggtcgtaag gtcgccgatc tcatgtgcca gcgtaaggca tagcttcgcg
     2821 aggaacaggg cctctttctc cgggcctgcg ccatctatcg cagccgcgag aaggtcgtaa
     2881 accttttcga gatcctcgaa aggaagcttg tcgtcttgct gtttcatcgc gtaacccgcc
     2941 caagcgatat attaagcgca tcttctattt cgtcgaccct cagagccacc cagcgtgccg
     3001 ctacgtgacg atccggccgg agcaggtaga agctgccgtc cgtcgcagca taggtatcgg
     3061 aaatacatcc ttcaatgtcc gggatcaccg taagcatttc ctccggcagc ttcttcctcg
     3121 atatcaagac aagtaagaaa tcgtgatcga tcgctgcgag cctgcgggaa agatccagga
     3181 tatcggaagg cacattctca ccatccataa aatatagacc ggtaaacccg agcccgaggt
     3241 aatccagcag gaaggcgcca ttggcggctt tacggttgac cgcggcggag cccgccatga
     3301 ctcctccctg gaattcgctg tccctgtgcc gataactggt cagcgggctg tcgccgtagg
     3361 tgtacggaac gacctgccgg gggtcggcaa acttttgcgt gaactcctgg gtcagcgaca
     3421 actgcagcac cgcttcccgc atcagcgcat agccgcggct gggtggcgtc atgaagcggg
     3481 agcttttgcc ggcgttgcta aagatatcaa gtgttgcgcc gcggcgttcc ggggtgaagc
     3541 tttcgagcaa tccctcgccc gcttcgcctt tcaggacata agccagcttc cacgccgcgt
     3601 tcatcgcatc gtcgaacccg ttgttcaggc cgcgcacgcc gaagatggga acgatgcgcg
     3661 ctgcatcccc gataaacagg atacgcccat gtcggtaatc gtcgagacac agcgtgttcg
     3721 ctgtgtagat gctccaccat tccagctccc accgccctgc atgacccagc atgtccagga
     3781 tcgccgcaac ccgtgagcgc acgttctctt cggcaatcgc ctggtcggga ttttcgtgtg
     3841 gctgtaattg ccaatcgatg cgccagatat cgtccggctg ccggtgcacc agtatggtcg
     3901 agtccgggtt cgccctggat ccgaaaaacg agcgcc
//
LOCUS       DASUOM010000095         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_699920,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000095 DASUOM010000000
VERSION     DASUOM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699920"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFY27_01950"
     CDS             complement(<1..557)
                     /locus_tag="VFY27_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5764303.1"
                     /translation="MMKAMMAIATLLGAVAQLQAQQGAPITAIQLPEQLTLEEAISVA
                     LRNNGQYLRAANDAGVLEAQSRRAFSRLLPVPGASIGTQAARSGSMGPFGSSTSSSSG
                     TSLSLNLNMLIFDGGQTLQNYRNSKTAIQRGTLDLADAENNIRAAVVNAYYRAQLADS
                     AIALERRLLADRKTELEVAELRFRKA"
     gene            complement(615..1829)
                     /locus_tag="VFY27_01955"
     CDS             complement(615..1829)
                     /locus_tag="VFY27_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5764304.1"
                     /translation="MANPRRLVTILFFLVVAAGLGFGGLKKVQAINDKKKADSTNAAA
                     SKAPSPARPDSTVFTFGAGAAIPVVGAEVKRMPLIIWVSAMSVAASSHMVPVSAEAGG
                     RVERFLPKEYDTIKPGDTVMMLDTTELVFALRQSQNSYNRAEVQFKNSTLSDYLIKDP
                     AIRKEREINARLASGLEEAQLSLTRAKLEYAKAATRAPVGGRVANVKVFPGQRVSAGA
                     ELLTLIALDPILVQAEVLQSEVGKLRIGNQARIKFGSETDTLYTGTIQSINPMVDPVN
                     RTARVTVTVRNPKGTIIPGMTARVELQSRTYNDKLLVPKKAILQRSEGRNMLFVYIPN
                     KDTPGEGTTEWRYVNLGLSNNEFVEILPGEDGSQKLVEPGEIVLVDGHYTIEHQTPIR
                     LVDKLAPGMKRL"
     gene            complement(2028..>2845)
                     /locus_tag="VFY27_01960"
     CDS             complement(2028..>2845)
                     /locus_tag="VFY27_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764305.1"
                     /translation="VRIPVDAYLTGFSVTLIDADGKAAPNALIHHLNFIDPENRELFL
                     PISQRMLAIGKETGSYSFPKMLFGYPIRLGQPMVVSAMLHNPHVQDYRGVTVRVAFSY
                     TPTKRPFPLFRVYPFQLDVLFPHGDKSFDLPPGRSERSFEATPVIDGRIIAVSGHVHE
                     HAEALSLTDVASGRVLWSGSPTLDSAGNVARMPVTSFAANLGLPLKAGRNYRVTVVYN
                     NPGADTIRAGGMGVVGGVFLPNLLARWPKADPNDETYVIDRAHYLQQQTRVSR"
BASE COUNT          543 a          820 c          833 g          649 t
ORIGIN      
        1 gccttgcgga accgcagctc cgccacctcg agttccgttt tccggtcggc caggaggcgg
       61 cgctcgagtg ctatagcgct gtcggccagc tgcgcccggt agtaggcgtt gacgaccgcc
      121 gcccgtatgt tgttttccgc atcggccagg tcaagcgtac cacgctggat cgcggtcttg
      181 ctgttgcggt agttctgcag cgtctgacca ccgtcgaaga tcagcatgtt gaggttgagc
      241 gacaggctcg ttccgctcga tgaagatgtc gaagaaccga atggccccat gcttccgctg
      301 cgagccgctt gtgtaccgat gctcgcgccg ggaacgggaa ggaggcggga gaaggcgcgg
      361 cggctctggg cctccagaac tccagcatcg ttcgccgccc gcagatactg accattgttg
      421 cgaagcgcaa ctgaaatggc ttcctcgagc gtgagttgct cggggagctg tattgctgtg
      481 atgggcgcgc cctgctgcgc ctgaagttgt gcgacagcgc ccagcagcgt cgcgatggcc
      541 atcattgctt tcatcatatt cctgtcctgg cagactgagt tatttgcttc ttcaacgctg
      601 agcgcaccgc gcgactaaag ccgcttcatg ccgggcgcca gcttgtccac cagtcgaatc
      661 ggagtctgat gttcgatggt atagtgaccg tccaccagaa ctatttcgcc aggctccacc
      721 aacttctgag agccgtcctc gccgggaagg atctccacga actcgttgtt gctcagtccc
      781 aggttcacat agcgccactc cgttgtcccc tccccaggag tgtctttgtt ggggatgtaa
      841 acaaaaagca tgttgcgtcc ttcggagcgc tgcaggatcg ctttcttcgg aacgagcaac
      901 ttgtcgttgt aagttcgcga ttgcagctcc acgcgcgccg tcatcccggg aatgattgtt
      961 cccttggggt tccgcactgt aactgtcacg cgagcggtgc gattcaccgg atcaaccatg
     1021 ggattgatcg actggattgt tcccgtatac agggtatcgg tttcagatcc aaacttgatg
     1081 cgtgcctgat tcccgattct gagttttcca acttcgctct gcagaacctc ggcctgcacg
     1141 agtatgggat cgagcgcaat cagggtcagt agctcggcgc ctgctgaaac gcgctgtccc
     1201 ggaaaaacct ttacgttcgc aacacgtccg cccacggggg cgcgggttgc ggccttggcg
     1261 tactcgagct tcgctctcgt cagactcagc tgggcctctt cgagtcccga tgcgagccgc
     1321 gcatttattt cccgttcctt tctgatcgcc ggatccttga tcaggtaatc ggaaagcgtg
     1381 ctgttcttga actgaacttc agcgcgatta tagctgttct gcgactgcct gagcgcgaag
     1441 acaagttcag tcgtgtcgag catcatgacc gtgtccccgg gctttatcgt gtcgtattcc
     1501 ttcgggagga atcgctcgac gcgcccgccc gcctcggcgc tcaccggcac catatgggac
     1561 gaggctgcca cgctcattgc acttacccag ataatgagtg gcatgcgttt gacctcagcc
     1621 ccgacaacag ggatggcggc tccggcgcca aaggtgaaaa ccgttgaatc gggtcgcgct
     1681 ggcgatggtg ccttgctggc ggcggcgttc gtggaatcgg ccttcttctt gtcgttgatt
     1741 gcctggacct ttttcagccc accaaagccg agtcctgcgg caacgacgag gaagaagagg
     1801 atggtgacga ggcgacgtgg gtttgccatg gttaaactcg acttcaattg gttcggtggg
     1861 actgcttcag gtaacggtac gaaaggttac gggctataat gctgcgccga acgcggatac
     1921 gtccagtgcc tcaggagaga gtgtactgtc cctcccaaat acagagactt caatagcctc
     1981 ttagaccctg tgcttcatgc acagggttgt atttccttgc caggccgcta tcgcgagacg
     2041 cgcgtctgct gctggagata atgcgcgcgg tcaatgacgt aagtttcatc gttcgggtct
     2101 gccttgggcc acctcgcgag aagattcggc aggaagacgc ccccaactac tcccatgccg
     2161 ccggccctga tcgtgtcggc tccgggattg ttgtacacaa ctgttacacg ataattacgt
     2221 ccggccttca ggggcaatcc caggttggcg gcaaagctgg ttacgggcat tcgcgccacg
     2281 ttacctgcgg aatccagcgt cggtgacccg ctccaaagaa ccctgccgct tgcaacgtcg
     2341 gtgagactga gtgcctccgc gtgctcatgg acgtgcccgc tcacggcgat gattcgaccg
     2401 tcaatcaccg gtgtggcctc gaacgatctt tcgctccgtc ccggcggcag atcgaacgat
     2461 ttgtcaccat gcggaaagag cacgtcaagc tggaacggat acactcgaaa gagcggaaat
     2521 ggccgtttcg ttggcgtgta ggaaaaagcg actctcaccg taaccccgcg gtagtcctgc
     2581 acatgggggt tatgaagcat tgcggacaca accattggct gtcccagcct gatgggatag
     2641 ccgaataaca tctttggaaa gctgtaggat ccagtttcct tgccgattgc cagcatccgc
     2701 tgggaaatgg ggagaaacag ctctctgttt tcaggatcta tgaagttcag gtggtggatg
     2761 agcgcattcg gcgcggcttt tccgtctgca tcgataagag ttacgctgaa cccggtgagg
     2821 tacgcgtcca cgggaatccg cacct
//
LOCUS       DASUOM010000096         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1719298,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000096 DASUOM010000000
VERSION     DASUOM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719298"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFY27_01965"
     CDS             complement(<1..569)
                     /locus_tag="VFY27_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5764306.1"
                     /translation="MGEKLQLDDHVWVDRLLPWFVNNTLDDDERDRVRRHLDICDACR
                     ASVCLLSTVRDSVHHGTATPMVPRPRADRLLETIDSHVSKGRRTRTLAMFASAASVAA
                     ALVLVNLLLPDREQAVTEPARYETTTSPVQHASMDYVLDVQFEAGVPAAVQQQVLEGL
                     EAKEINSAGSDGAYRVTVNLPASSLEELER"
     gene            complement(562..1152)
                     /locus_tag="VFY27_01970"
     CDS             complement(562..1152)
                     /locus_tag="VFY27_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5764307.1"
                     /translation="MRDTAETKPGDQDQRELALLKRIAQRDKDAISDLYQLYHPRLFK
                     FVFRLTHSYAAADELVNDIMLIVWQQAAGFRGDSKVSTWIFGIAYRQAIRSLTRGRKA
                     PAQHGCAEEPTYDDSIEVETRDWVWRGLHTLPYPQQLSMVLVFYYGLSYAETAQIAGC
                     SVNTIKTRMFHARRKLRDYFAEAAIDNAMEDGRQHG"
     gene            1353..2573
                     /locus_tag="VFY27_01975"
     CDS             1353..2573
                     /locus_tag="VFY27_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764308.1"
                     /translation="MRNHQFFPNRAVPALLLATALAAGCGGGDGSSGAEPPPNPPPPS
                     PPPFGANFSEIQAEVFTPTCAVSGCHTGAAAPQGLRLDEANSYGLLVDVASMEVPSLL
                     RVEPGDPDNSYLIQKLEGTADVGEQMPFGGTPLPQASIDVIRQWIIEGAVDDRAPSGE
                     PIRVSSLSPVPDSELEAPPEEIVAMFDRELDVSTVTAETFILEASGGDGTFGDGNEEP
                     IAAAAITTPDTNPTSATFDLGGVAIGDDTYRVRLLGSGASVILDLDANALDGEFSGTF
                     PSGDGTEGGDFEALFTVAAPAPMGPTLDEIQGSVFTPTCSGCHTGPTGETLPGGMDLT
                     NADASFANLVGVPSLQVPELSRVAAGDPDNSYLIHKLEGTASQGAQMPFGGPPLDQAV
                     IDDIRQWIADGAER"
BASE COUNT          490 a          821 c          836 g          504 t
ORIGIN      
        1 cgttcgagct cctcgagcga ggaggccggc agattgacgg taacccggta ggcgccgtca
       61 gatccggccg agttgatctc ctttgcctcc aggccttcca gcacctgctg ttgcacggcc
      121 gccgggaccc cggcttcaaa ctgcacatcg agaacgtaat ccatcgacgc gtgctgcacc
      181 ggcgaggtcg tggtttcgta gcgggcaggt tccgttaccg cctgctcccg atccggcagc
      241 aggaggttga cgaggaccag tgcggcagca acggatgccg cagacgcgaa catcgccagc
      301 gtgcgcgtgc gcctgccttt gctcacatgg ctgtctatcg tttcgagcag tctgtcggcc
      361 cgcggcctgg gtaccatggg tgtcgccgtt ccgtgatgca cactgtcgcg caccgtcgac
      421 agcaggcaca cggatgcgcg gcaagcatcg cagatgtcga ggtgacgacg cacgcggtcc
      481 cgttcatcgt cgtcgagcgt gttgttgacg aaccacggca acagccggtc gacccacaca
      541 tgatcatcca gctgaagctt ctcacccatg ttgcctccca tcctccatcg cgttatcgat
      601 cgctgcttcg gcaaagtaat ctcgcagctt gcgccgtgcg tgaaacatcc ttgtctttat
      661 ggtgttgacg gagcaacccg cgatctgggc ggtttccgca taggaaagtc cgtagtagaa
      721 gaccagtacc atgctcaact gttgcgggta cggcagcgtg tgaagccccc gccataccca
      781 gtcccgggtt tcgacctcga tgctgtcatc gtacgttggt tcctcggcgc agccgtgttg
      841 cgctggtgct tttcttccgc gagtcaggct gcgtatggcc tggcgatagg cgatgccaaa
      901 gatccaggtg gaaaccttcg agtcgccgcg gaatcctgcg gcttgctgcc agacgatcag
      961 catgatgtcg ttgaccagct cgtcagccgc cgcgtaggaa tgcgtgagcc ggaaaacaaa
     1021 cttgaacagt cgagggtgat agagctggta gaggtccgaa atggcgtctt tgtcccgctg
     1081 cgcaatacgt ttcagtaacg ccagctcgcg ttgatcctgg tcacccggtt tcgtttcggc
     1141 ggtatctctc attgtgtagt cgatgtggcc cagcttgagt gagtgccatc cagcggaccg
     1201 gaaggttcaa tggacgaaaa agaaaaggac cgaaagaaat cgtcccggcc ggtgcgagtc
     1261 gaattccgca aaatgcgccg ccattttgaa cctgttcgcg acctggtggg cacttaacta
     1321 acgtgatact ccacaacgga acggcaggca atatgcggaa tcatcagttc ttcccgaatc
     1381 gggctgttcc ggctttgctg ctggcaaccg cattggcggc cggttgcggc ggcggcgacg
     1441 gctcctctgg tgcagagcct cccccgaacc cgccaccacc ttcaccgccg ccgttcggcg
     1501 caaatttctc ggaaatccag gccgaggtgt tcaccccgac ctgtgcggtt tccggctgtc
     1561 acaccggcgc cgccgcgccg caaggtttgc ggcttgacga ggcgaacagc tacggcttgc
     1621 tggtcgacgt tgcaagtatg gaggtgccgt cgctgctgcg cgtcgaaccg ggcgacccgg
     1681 acaacagcta cctgatccag aaactcgaag gtactgcgga tgtcggggaa cagatgccgt
     1741 tcggcgggac gcctctcccg caggcgagca tcgacgtcat ccgccagtgg atcatcgaag
     1801 gggcggtgga cgaccgggca ccatccggtg agccgatccg ggtgagctcc ctttctccgg
     1861 tgcccgacag cgaactggag gcgccgccgg aggaaattgt cgccatgttc gatcgtgaac
     1921 tggatgtttc gacggtcacc gccgaaacgt tcatcctgga agcgagcgga ggggacggga
     1981 ccttcggtga cggcaacgaa gagccgatcg cggcggccgc gatcaccaca ccggatacga
     2041 acccgacgag tgcaacgttc gacctgggcg gcgtggctat tggcgacgat acctatcgtg
     2101 tccggctgct aggcagcggc gcctctgtga ttctcgacct ggacgcaaat gcgctggacg
     2161 gcgagttctc gggaaccttc ccgtccggag acgggaccga aggcggcgac ttcgaagccc
     2221 tgttcactgt cgcagcgccg gcgccgatgg gcccgacgct cgacgaaatc cagggcagcg
     2281 tctttacacc gacttgcagc ggatgccata ccggcccgac cggtgagacg ctgccgggcg
     2341 gcatggacct gacgaatgcc gacgcgagct ttgccaacct ggtcggcgtc ccgagccttc
     2401 aggttccgga gctatcgcgc gtggcagcgg gcgatcccga caacagttac ctgatccata
     2461 agctcgaggg aacggccagc caaggtgcgc aaatgccgtt cggcggccca ccgctggacc
     2521 aggccgtcat cgatgacatt cgccagtgga tcgccgacgg cgcggagcgt tagcggcggc
     2581 tctgcggaat gagcacctgg aaatgcggca cggccgtgct ggcccttgta ctgctggcgg
     2641 gcttcgccac t
//
LOCUS       DASUOM010000097         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1517731,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000097 DASUOM010000000
VERSION     DASUOM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517731"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VFY27_01980"
     CDS             <1..1683
                     /locus_tag="VFY27_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5764309.1"
                     /translation="NDGVLRRLSLWQFESGVMFPSLPLAIALSRPETAASSRISSGDD
                     AVYLSNYRQVQRYAPEEILAADFPEGNIEGRTVFLDSALPLVHAAAILPSGEYVTRSE
                     ITAALLANIEQDEAIVAPAWVGALEWLAPALLAIIGVLFGPGRKRRDILIWAAGLVAA
                     LILIEALMLAFGRVRFDLGRPIIVFIAAAASSWWLAGVKKKAAVDAFKRGSDFLSAGR
                     LEPAFAEFRRCEPSDTVAAVMYKLSLAFEGQAKPERAEAVLQWLKQTQGQASDRSHTL
                     HDTNGNGMPERLGRYVIERKLGRGAMGAVYLARDPRINRPVALKVIPIEKEFEDEELE
                     EARLRFFREAESAGRLTHPNIITVFDAGEDKHLAYIAMEYLGGTPLTHYTDPKKLLAP
                     KKTLELCARTADALNYAHDQGVIHRDIKPANLLYDLKSDLLKISDFGVARLTDNNRTK
                     TGIVLGTPMYMSPEQLGAETLTGHSDLFSLGVTLYELLTGEVPFKAKNIAVLMTKITS
                     EDPVPVSNRRPGIPPSVDAVLSKALAKRPEKRFANGAEMAIALRNCATYASA"
     gene            1697..2524
                     /locus_tag="VFY27_01985"
     CDS             1697..2524
                     /locus_tag="VFY27_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5764310.1"
                     /translation="MSLRSKIKFAEITDTGKVREHNEDAIGSNADIGLMVLADGMGGY
                     NAGEVASGIAVQTITELAAEGATREKRNEIDSTTGLMRQSIVLRDAVARANKIIFQTA
                     QSQTHCEGMGTTLVTAMFYDNKVSIAHVGDSRAYRLRGEKFEQLTLDHSLLQELVDRG
                     FYSEEEAQRSTNRNYVTRALGVEPSVEVEVHEYDVLPNDVFLLCSDGLPDMVEDEDIH
                     LTISTFNASLDVVGQQLVQLTNDNGGRDNVSVMLAQILEPFAAKRSLAARLTGMFRG"
     gene            2600..>2927
                     /locus_tag="VFY27_01990"
     CDS             2600..>2927
                     /locus_tag="VFY27_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5764311.1"
                     /translation="MARLILSLDSQVLAEYNMSKERYTIGRLPDNDVRIDNPAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHALQHGDVITIGHHQLRFSDQHTGDVEQ
                     DEFEKTM"
BASE COUNT          648 a          860 c          874 g          545 t
ORIGIN      
        1 aatgacggcg tcctgcgcag gctttcgcta tggcagttcg agagcggcgt catgttcccg
       61 tcgctgcccc tggcgatcgc gttaagccgt ccggagaccg ccgcgagttc gcgcatctcg
      121 agcggcgacg acgccgtcta cctgtccaat tatcggcagg tacagcgtta cgcgcccgaa
      181 gaaattcttg cagcagattt tccggagggg aacatagagg gtcgcaccgt tttcctcgac
      241 tcggcactgc cgctggttca cgccgcagcc attttgccat cgggagagta cgtgacccgc
      301 tcggagatca cggcagcatt gcttgccaac atcgagcagg acgaggcgat tgtcgcgcca
      361 gcatgggtcg gggcgctcga gtggcttgcg ccggcgttgc tggcaatcat cggcgtgctg
      421 ttcgggccag gccgcaaacg ccgcgacatt ctcatctggg cggcgggcct ggtggctgca
      481 ctgatcctga ttgaagctct catgctcgcc ttcgggcgcg tgcgcttcga tcttggtcgt
      541 ccgatcatcg tgtttatcgc agccgcggct tccagctggt ggctcgcggg agtgaagaaa
      601 aaagccgccg tagatgcgtt caagcgcggt tcagacttcc tctcggcggg acggctggag
      661 cccgcattcg ccgagttccg ccgttgcgaa ccctcggaca ccgtcgcggc ggtgatgtac
      721 aagctgtcgc ttgctttcga ggggcaggcg aagccggaac gggcggaggc tgtgctgcaa
      781 tggctgaaac agacccaggg gcaggccagt gacagaagcc atacgctaca cgacaccaat
      841 ggcaatggca tgcccgagcg cctcggccgc tatgtcatcg aacgcaaact gggccgcggc
      901 gcgatgggcg ccgtctacct tgcccgggat ccgcgaatca accggccggt agcgctcaag
      961 gtcattccta tagagaaaga gttcgaggac gaggaactgg aagaagcgcg gctgcgattt
     1021 ttccgcgaag cggagtccgc cggacgcctg acgcatccca acataatcac ggtattcgat
     1081 gccggcgagg acaagcacct cgcgtacatc gcgatggagt acctgggcgg cactccgctg
     1141 acgcactata ccgatccaaa gaagctgctg gccccgaaaa agacactgga gctttgcgcc
     1201 cgtactgccg atgccctcaa ctacgcgcat gatcaggggg tcatccaccg ggacatcaaa
     1261 ccggctaacc tgctctacga cctgaagtcc gacctcctga aaatcagcga cttcggagtc
     1321 gcccgcctta cggacaacaa caggaccaag accggcattg tccttgggac gccgatgtat
     1381 atgtccccgg agcagcttgg cgccgagacc ctcaccgggc actcggactt attttcgcta
     1441 ggtgttactc tatacgagct tttgaccggc gaagtgccgt tcaaagcgaa gaacattgcg
     1501 gtgttgatga ctaagattac atccgaagac cccgtaccgg tatccaatcg cagaccaggc
     1561 atccccccca gcgtggatgc cgtgctgtcg aaggcgcttg cgaagcgccc ggagaagcgg
     1621 ttcgcaaacg gagccgaaat ggccatcgcc ctccgcaatt gcgcgacata cgcgtccgcc
     1681 tgataaagaa aaccatatga gcctgcggtc gaagatcaaa tttgcagaga tcacggatac
     1741 cgggaaggta agggagcaca atgaagacgc gataggctcg aatgccgata tcggcctgat
     1801 ggtgctcgca gacgggatgg gcgggtacaa cgctggtgaa gtagccagcg gtatcgcggt
     1861 acagacaatc accgaacttg ccgcggaggg cgcgacccgc gagaagcgca acgagatcga
     1921 ctccacaacg gggctgatgc gccagtcgat cgtgcttcgc gacgccgtcg cccgcgctaa
     1981 caagatcata ttccaaaccg cccagagcca gactcactgc gaaggcatgg gaacgacgct
     2041 ggttacggcc atgttctacg acaacaaggt gtcgatcgcc catgtcggcg attccagggc
     2101 ttaccggctg cgcggcgaaa agttcgaaca gctcacgctt gaccattcgt tactgcagga
     2161 actggttgac cgcggattct attcagaaga agaagcgcaa cgttcgacca acaggaacta
     2221 cgtgacccgc gcgctggggg tggagccatc ggtcgaggtc gaggttcacg agtacgacgt
     2281 tctgccgaac gacgttttcc tgctgtgctc cgatggcctg ccagacatgg tcgaggatga
     2341 agatattcac ttaactatca gcacttttaa tgctagcctt gatgtggttg gtcaacaact
     2401 ggtgcaacta accaacgata atggcgggcg cgacaacgtt tccgtaatgc tggcgcagat
     2461 cctggagccg ttcgcggcca agaggagcct ggccgcccgc ttgacgggta tgtttcgcgg
     2521 ataggggagg tggggtgacc tggggtctgc cccagcgcac cctgcgtgta ttggtcttac
     2581 agaaaacggc aagaataata tggcacgttt aatcctgagt ctggacagcc aggtgctggc
     2641 cgaatacaac atgtcgaaag agcgatacac gatcggtcgt ctcccggaca acgacgttcg
     2701 catcgacaac ccagccgtca gcggccacca ctcgctcatc atcaatattc tgaacgactc
     2761 gtttctcgag gacttaaaca gcaccaacgg cacgtacgtc aacggcaagc tgatcaagaa
     2821 acatgccctg caacatggcg acgtcatcac tatcggtcac catcagctcc gtttctcgga
     2881 ccagcacacc ggtgacgtcg agcaggacga gttcgagaaa acgatgg
//
LOCUS       DASUOM010000098         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1414833,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000098 DASUOM010000000
VERSION     DASUOM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414833"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFY27_01995"
     CDS             <1..1055
                     /locus_tag="VFY27_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5764312.1"
                     /translation="TGDAAEDPEPVDVTGVPPVDAGSGATPGEDPVVSQEQPPGASTV
                     PQQDTSAEDPFVEAPIEIEEEGAPAAGSPRGEVVGQPATEQPPPQQPAVTGTGTPSTL
                     PAATAIVPLASPGSFRTEADVTLREGEDELAIDLVRQVDLGTPLSVRLEEAGFSGESS
                     PSQAGRYRLSNNGIAVFEAGQGRARINVSMPSDDVRNPDVQAALLVRNIEDPGSELAL
                     INLVLEDDDQRAFEAALPQDTVGFATSRMYVSERDPAIQIDVLRYNPGGGELEIRYFI
                     GGGSATEGEDYFLPGSTGLVFEPGQRSARLLIPLVQDSVLEADETFSLELRSSTEPAQ
                     ANISRRIEVIIRDDDG"
     gene            1098..>2182
                     /locus_tag="VFY27_02000"
     CDS             1098..>2182
                     /locus_tag="VFY27_02000"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5764313.1"
                     /translation="MEEKELRRYSRIVVDGVEQAPSRAMLRAVGFEEADFKRPQIGIA
                     STWSMITPCNMHIDQLARQTAAGADRAGGKGVIFNTITVSDGISMGTPGMRYSLVSRE
                     IIADSIEAVAGAQGFDGLVAIGGCDKNMPGCVMAMARLNRPSVFVYGGTIRPGVNRRD
                     IVSVFEAVGAAAAGKISSDELLEVERTAIPGPGACGGMYTANTMASAIEALGMSLPNS
                     SAQEAISSHKADDCMRAGAAVVELIRSGTTPSDILTREAFENAITVVIALGGSTNAVL
                     HLLAVAHDAGVRLDIDDFTRVGARVPVVADLKPSGRYLMSELIAIGGIRPLMRGLLER
                     GLLHGDCLTVTGRTVAENLVDAPSYPEG"
BASE COUNT          420 a          674 c          708 g          380 t
ORIGIN      
        1 gtactggcga cgcagcggaa gatcccgaac ctgtcgatgt gaccggtgtt ccgcctgtgg
       61 atgccggcag cggtgcgacg ccgggggagg acccggtcgt gtcgcaggag cagccgccag
      121 gcgcttcgac cgtgccgcaa caggatacgt ctgcggaaga tcctttcgtc gaagccccca
      181 tagaaatcga ggaggagggc gctcctgcgg ccggttcacc gcggggggaa gtggttgggc
      241 aaccggctac cgaacagcct ccgccacagc agcctgccgt caccggaaca ggcacccctt
      301 cgacgcttcc cgcggcaacg gccatcgtgc cgctggcgag tcccggctca ttccgcacgg
      361 aagctgacgt gaccttgcgc gagggcgaag atgagcttgc catcgatctc gtgcgccagg
      421 tcgacctcgg cacacccctc tcggtacggt tggaggaggc cggtttcagc ggcgagagct
      481 cgccatccca ggccgggcgg taccgcctct cgaacaatgg tattgcagtt ttcgaggctg
      541 gccagggaag ggccaggatc aatgtgtcga tgccctccga cgatgtgcgg aatcccgatg
      601 tacaggccgc gcttctcgtc aggaatattg aagacccggg ctccgaactt gcgctgatca
      661 acctcgtgct ggaggacgac gaccagcgtg cgttcgaagc ggcgcttcca caggacacgg
      721 ttggttttgc gacgagccgc atgtatgtca gcgaacggga tccagcaata caaatcgatg
      781 tgctgaggta caaccccggc ggcggcgaac tcgagatccg ctacttcatc ggcggcggaa
      841 gcgctacaga aggcgaggat tactttctcc cgggctcgac cggtctcgtc ttcgaacccg
      901 ggcagcgttc cgcgcgcctg ctcatcccac tggtgcagga ctccgtcctc gaagcggacg
      961 agacattttc gctggaactc aggagtagca ctgaaccagc ccaggccaac atctctcgtc
     1021 gcatcgaagt tatcatccgg gacgacgatg gctgacacca caacaattcc ggcggagccg
     1081 gcagagcgaa aataagaatg gaagaaaagg aactgcggcg gtactcgcgt atcgtcgtcg
     1141 acggcgttga gcaagcgccg agccgggcga tgctgcgcgc ggtggggttc gaggaagctg
     1201 acttcaagcg tccgcagatc ggaattgcct ccacctggag catgatcacg ccatgcaaca
     1261 tgcatatcga ccagctcgcg cgtcagacgg ctgccggcgc tgacagggcc ggcggcaagg
     1321 gcgttatctt caataccatt accgtctccg acggcatctc tatgggcaca cccggcatgc
     1381 gctattcgct cgtctcccgg gaaatcatcg cagattcaat cgaggcggtt gccggtgcgc
     1441 agggattcga tggcctggtg gccatcggcg gctgcgacaa gaacatgccc ggatgcgtga
     1501 tggccatggc gcggctcaat cggccgtcgg tgttcgtcta cgggggcacc atcaggccgg
     1561 gtgtcaatcg gcgcgacatc gtatccgtgt tcgaggcggt cggcgccgcc gccgcgggca
     1621 agatcagcag cgacgaactc ctggaagtcg aacggaccgc cataccgggt cctggcgcct
     1681 gcggtggcat gtacaccgcg aacaccatgg cgtcggcaat cgaagcgctc ggcatgagtc
     1741 tgccgaacag ctcggcacag gaggcgatat cctcgcacaa agcggacgat tgcatgcgtg
     1801 ccggcgcggc agtcgtggaa ctgatcagaa gcggcacgac gccgagtgac atcctcacca
     1861 gggaagcatt cgagaatgcg attaccgtcg tcatcgctct cggcggctcc accaatgccg
     1921 tgttgcactt gctggccgtc gctcacgacg ccggtgtgcg gctggacatc gacgacttca
     1981 cgcgagttgg tgcacgcgtt ccggtagtgg ccgatttgaa gccgagcggg cgctatctca
     2041 tgtccgagct gattgcgatc ggcggtatcc gtccgctgat gcgcggactg ctcgagcggg
     2101 gcctgctgca cggcgactgc cttaccgtca ccggcagaac ggtcgccgaa aaccttgttg
     2161 atgctccatc gtacccggaa gg
//
LOCUS       DASUOM010000099         6975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1675306,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000099 DASUOM010000000
VERSION     DASUOM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675306"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFY27_02005"
     CDS             <1..1215
                     /locus_tag="VFY27_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5764314.1"
                     /translation="SSVAVIGAGTMGAGIAYGCLAAGFGVRLLDKDEASLGRGLATIE
                     KLFEGGIARGKIDRREMQAAMERFQAVSDFAGLKEVDMVIEAVFENMAIKKEVFSRLD
                     ASCKHGAILATNTSTLDVDEIASATSRPQDVIGLHFFSPAHIMRLLEIVRGRETSGDV
                     IATSLALARKLGKTGVVVGNCFGFVGNRMLYSYGRENQLLLLEGAAPEYIDKVLRDWG
                     MAMGPNAVGDLAGLDVGYKVRQERKDLPADPRFYRVADMLAELGRFGQKTGKGIYRYE
                     AGSREPVPDPEVRELIRNEAKRLGVEQRNIGESEIIERCIYGLVTEGARILEDGIASR
                     SSDIDVIWINGYGFPRHRGGPMNFADMAGLSEVYAKVREFRERFGPMYWEPPELLQRL
                     AEEGGRFGDYVG"
     gene            complement(1265..2092)
                     /locus_tag="VFY27_02010"
     CDS             complement(1265..2092)
                     /locus_tag="VFY27_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764315.1"
                     /translation="MTLTPGSIKELAPGIRRLVAPNPGMMTGPGTNTWILGARDVAVI
                     DPGPAIDVHVDTIVDAVGVAIRWILVTHTHPDHSPAAALLAKRTGAAVCGRAAPDGQH
                     QDATFRPDRILEDGEVLSAGEFELDVIHTPGHASNHLCYRQARLCWLFTGDHIINGST
                     VVIDPPDGSMADYLQSLRRLKSFEIAAIAPGHGEVIDEPYAAIDWIIEHRLAREAKVA
                     AAVALHPNLTARGLVPHVYDDVDESLHDIAERSLSAHLEKLELDKRVVREGDCWRPL"
     gene            complement(2146..2703)
                     /locus_tag="VFY27_02015"
     CDS             complement(2146..2703)
                     /locus_tag="VFY27_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5764316.1"
                     /translation="MSRFQVRPSMTLPAVALALLRCSGATAEVIDAEASGFTTRHSVT
                     VSRPRAEVYKAATAGIGSWWSDDHTVSGRAANMYLEPRVQGCFCERLGEQGGVVHMLV
                     TFVNPAVMLRLSGGLGPLGLMGVSGNMTWEFTDSADGTTMTWSYAVGGYFPGGLDQIS
                     SAVDLVLTEQMNQFKKFAEESRATP"
     gene            2879..3802
                     /locus_tag="VFY27_02020"
     CDS             2879..3802
                     /locus_tag="VFY27_02020"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEX5764317.1"
                     /translation="MLDPEHKLKPAGTSTQEESVSALLSLFERFGKVTVLSGAGCSTA
                     SGIPDYRDDGGNWKHRRPVQLAEFLSSAATRRRYWAQSFAGWSRIANAKPNDAHLALA
                     ALERLQKVGCVITQNVDNLHRRAGSLNVIDLHGLLHQVRCLGCGVIVGRDQFQQQLAE
                     DNPDWSATVAGFAPDGDARLVAGEFRDFRIPECESCGGTWKPDVVFFGEGVPAERVER
                     ATGSVQECDALLIVGSSLMVLSGLRFARMANALGKAVIIINRGTTRADDLATAKLTDD
                     CGALLSSLVDRLTKEQNGDARQGNGRLDGSV"
     gene            3762..4661
                     /locus_tag="VFY27_02025"
     CDS             3762..4661
                     /locus_tag="VFY27_02025"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5764318.1"
                     /translation="MLDRGTDDWTVVFESRAYQPCSDRALVLHSLGIPYEIVRRLDGV
                     ALAVPAPFAERAKYEIWQYDQENRKKPPPPAPLPVENQNGVPGALVYIIVVCLVAWLA
                     SQGFLNRDWFAAGRVDGELIRQGEWWRTITALTLHGGTRHLLGNIGFGMLFGVLAGRL
                     VGSGVAWLGIVLAAGAGNWLNTILLDSSHRSVGASTAVFAALGLLAGFVWRAKLMSQE
                     RWPYRLGPVIGGIALLAFTGTGDENTDIGAHLAGFVCGFATGTALTLAAQQLANPRLQ
                     KLCGAAALMLVLAAWLKALTWEA"
     gene            4730..5776
                     /locus_tag="VFY27_02030"
     CDS             4730..5776
                     /locus_tag="VFY27_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5764319.1"
                     /translation="MALHKGRGALSRTAGRFETRSIEATEPADDVAPETVLTAMEARR
                     IISSNDSPDVPFERSINPYMGCEHGCIYCYARPSHSYLDLSPGIDFETRIFYKPNAVK
                     ALLAEWQKPGYVCKPITIGANTDPYQPAEKKLELTRQLLQQFLAHRHPVSLISKSGLM
                     RRDLDLLAELAHLKLCSVAISVPTADNALKRRLEPRVPSADLRFRLIEALSSHGVPTS
                     LLMAPIIPAVNDREIEDIVSRAAAAGARRASFVLLRLPFELKQIFSEWLEGHMPDRAS
                     HVMSLVRQASGGKEYDNRFGIRQSGSGAYADMIAQRFRVACTKSGIPNQRSLDELDCS
                     SFEPPGQGQMTLVL"
     gene            5854..>6975
                     /locus_tag="VFY27_02035"
     CDS             5854..>6975
                     /locus_tag="VFY27_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5764320.1"
                     /translation="MKATKFSSPFGLGAALFIMAACGLAGAQEPPPETEELEEITLDR
                     SPGGTPDAVSEQVSEEEAPEERLQRYFMLYKEALESKAYAEADTLAKQVVELSIGLFG
                     INSLDSARALTNLGIAQHQNEDYDSAILNYTAAIDIIERIEDRLNGNLINPLKGLGAA
                     QLAAGRPDEAVSSFERAVHVSHVNEGPHNLMQVDVLESLAETYLSVGELDEVKDIQEH
                     IFSLQARDVELDSLEAIPALERQARWQHRLQLYEQERYTWRKIIDIIEDRRSDEDLSL
                     IPALTELGKSYLFIGQPNMAFHQPTSISSGEIYLKRALRIAEENPDANWETLEGAMLA
                     LGDFYILSGKPSRARKIYNETWQLLSADEERLKNRQAHLE"
BASE COUNT         1379 a         2103 c         2226 g         1267 t
ORIGIN      
        1 tcgagcgtcg ccgtcatcgg cgcaggcacg atgggtgccg gcatcgcgta cggctgcctg
       61 gctgcgggtt tcggtgtgcg gttgctcgac aaggatgagg caagccttgg ccgcggcctt
      121 gccaccatag agaaactgtt cgagggcggt atcgcgcgcg gcaagatcga ccggcgcgag
      181 atgcaggcgg cgatggagcg attccaggcc gtgagcgact tcgcggggct gaaagaggtc
      241 gacatggtta tcgaagccgt gttcgagaac atggcaatca agaaagaagt tttttcccgc
      301 ctggacgcaa gctgcaaaca tggcgccatc ctcgccacga acacttccac gttggacgtc
      361 gacgaaattg catcggcgac ctcgcgtcca caagacgtca tcgggctgca cttcttcagc
      421 ccggcgcaca tcatgcgcct gctcgagatc gtacgcggca gggagacctc aggcgacgtc
      481 atcgctacat cgctggctct cgccaggaag ctcgggaaga ccggcgtggt ggtgggcaac
      541 tgtttcggct tcgtcggcaa ccggatgctg tacagctacg ggcgtgagaa ccagttgctg
      601 ctgctcgaag gggcggcccc ggaatacata gacaaggtgc tgcgtgactg gggcatggct
      661 atgggcccta atgcagtcgg tgatctcgcc ggactggacg tgggatacaa agtgcgccag
      721 gaacggaagg accttcccgc cgatccgcgt ttctaccgcg tcgcggacat gcttgccgaa
      781 ctgggccgct tcggccagaa aaccgggaaa ggcatttacc ggtacgaggc cggatcccgg
      841 gagcctgttc cggacccgga agtgcgggag ctcatacgga atgaagcgaa gcgcctggga
      901 gtcgagcaaa ggaacatcgg cgagagcgaa atcatcgagc gctgcatcta tgggctggtg
      961 acggaaggcg cgcgcatcct ggaggacggc atcgcgtccc gctcatcgga catcgacgtc
     1021 atctggatca acggttacgg ctttccgcgc catcggggcg gcccgatgaa tttcgcggac
     1081 atggcaggcc tgagcgaagt ttacgcaaag gtgcgcgagt tccgggagcg gttcgggccc
     1141 atgtactggg agccgccgga gttgttgcag aggctcgcgg aggaaggggg gcggtttggg
     1201 gactatgtcg gctgaacgcc gagatttagc taatcattct cgcactcgcc cgcatggcgg
     1261 gctcctacag tgggcgccag caatctcctt cgcggacaac gcgcttgtcg agttcaagct
     1321 tttcgagatg agctgacagc gagcgctccg cgatgtcgtg caggctttcg tcgacgtcgt
     1381 cgtagacgtg aggcaccagg ccccgcgccg taaggttcgg gtgcagagcc accgcagccg
     1441 cgaccttcgc ttcacgtgcc agccggtgct cgatgatcca gtcgattgcg gcgtagggct
     1501 cgtcgataac ctcgccgtgc cccggcgcga tggcggcaat ctcaaagctc ttgagtctcc
     1561 gcagcgactg caggtaatcc gccatgcttc cgtccggcgg gtcgatcacg acagtggagc
     1621 cgttgatgat gtggtcgccg gtgaaaagcc agcacaagcg tgcttgccgg taacaaaggt
     1681 ggttggaagc gtgtcccggt gtgtggatca catcgagttc gaattcgccc gcggagagga
     1741 cctcgccatc ctccaggatc ctgtcgggtc gaaaagtggc gtcctggtgc tgtccatccg
     1801 gcgcggcacg tccgcacacg gcggcgcccg tgcgtttcgc cagcagcgcc gctgccggtg
     1861 aatgatccgg gtgcgtgtgg gtgacgagga tccagcgtat ggccactccc accgcatcga
     1921 cgatcgtatc gacgtggaca tcgatagcag gaccggggtc tataactgcg acatcccgcg
     1981 ctcccaatat ccacgtgttg gtgccgggcc cggtcatcat gcccgggttg ggagcgacga
     2041 ggcgccggat tcccggcgcg agttccttga tggatcctgg cgtcagagtc atttcatcac
     2101 cgggtttgac tggatcggcc ggctcgcacc ggcttccctc gcctgttaag gggtcgcccg
     2161 ggactcctcg gcaaatttct tgaactggtt catctgctct gtcagtacca ggtccacggc
     2221 gctcgagatc tggtcgaggc caccgggaaa gtagccgcca acggcatagc tccacgtcat
     2281 tgtggtgcca tccgcgctgt ccgtaaactc ccaggtcatg ttgccactga cgcccatgag
     2341 cccgagaggg cccagcccac cggacaggcg cagcatgacg gccgggttta cgaaggtaac
     2401 gagcatgtgc actaccccac cctgttcacc cagcctctca cagaagcaac cctgtacgcg
     2461 cggctcgagg tacatgttgg ccgcgcgtcc ggagacggtg tgatcgtcgc tccaccacga
     2521 gccaattccg gctgtcgccg ccttgtatac ctcggcgcgc ggccgcgaaa ccgtgacgct
     2581 atgacgggtg gtgaaaccgg atgcttcggc gtcgatcact tctgccgttg cgcccgaaca
     2641 gcgaagcagc gccagcgcga cggcaggcaa ggtcatcgac ggtcggacct ggaacctgga
     2701 catggtttga acaacttgag tggtcagaag gccacccaat gttcggtcaa aagaacggga
     2761 ctggcaacag ggacgcggca aggaaaccgg ccgacagcag gaccagtccg aagtacaatt
     2821 ttcgccacgt ccgcatgttc ccctaagccc tcgatcttcc agtgcaatgc gattatcgat
     2881 gttggatcct gaacacaaac tgaagcccgc cggaacgagc acgcaggagg aatccgtttc
     2941 tgccttgctc tcgctgtttg agaggttcgg caaggtcacc gtccttagcg gcgccggttg
     3001 cagcacggcg tcgggcatac cggattacag ggacgatggc ggaaactgga agcaccgcag
     3061 accggtgcag cttgcggaat tcctttcaag tgccgcgacg cgacggcgtt actgggctca
     3121 gagcttcgct ggctggagcc ggatcgcaaa cgcgaaaccg aacgacgctc acctggcgct
     3181 ggccgcgctc gaacggctgc agaaggtggg ctgcgtaatt acgcagaacg tcgacaatct
     3241 ccaccggcgt gccggcagcc tgaatgtcat cgaccttcat ggtctcctgc accaggtccg
     3301 ctgccttggc tgcggcgtaa tcgtcgggcg ggaccagttc cagcagcagc tggcggaaga
     3361 taatcccgac tggtccgcaa ctgtcgccgg ctttgcgccg gacggcgatg cccgcctcgt
     3421 cgccggcgaa ttccgggact ttcgcatacc ggaatgcgag tcctgtggcg gcacgtggaa
     3481 gccggacgtc gtgttcttcg gcgaaggcgt tccggcggag cgggtggaac gcgcgaccgg
     3541 gtccgtccag gagtgcgatg cattattgat cgtcggttcg tcattgatgg tactttcggg
     3601 gctgcggttc gcccgaatgg ccaatgctct tggcaaggca gtcatcatca tcaatcgcgg
     3661 tacgacaagg gcggacgacc tggccaccgc caagctgacc gacgactgcg gcgcgcttct
     3721 ttcgtccctg gtcgaccgcc tgacaaagga acaaaacggg gatgctcgac aggggaacgg
     3781 acgactggac ggtagtgttt gaaagccgcg cataccaacc ctgctccgat cgtgcgctgg
     3841 tactgcattc cctcggcatt ccttatgaga tagtcaggag actcgacggg gttgcactgg
     3901 cggtcccggc tccgttcgct gaacgggcga aatatgaaat ctggcaatac gaccaggaga
     3961 accggaagaa gccgccgccg ccagcgcccc tgccggtgga gaaccagaat ggcgtgcccg
     4021 gtgccctggt ctacatcatt gtcgtgtgcc tggttgcctg gctcgccagc cagggtttcc
     4081 tgaatcgtga ctggtttgcc gcggggcgcg tcgacggcga actgatccgg cagggcgagt
     4141 ggtggcgaac gatcacggcg cttaccctgc acgggggtac gcgccatctg ctgggcaaca
     4201 taggatttgg catgctgttc ggcgtcctgg cgggacggct ggtcggttct ggcgttgcgt
     4261 ggctcggcat cgtgttggcc gcgggcgcgg gcaactggct caacaccata ctgctggatt
     4321 ccagtcaccg ctctgtcggt gcctctactg cggtgttcgc cgctctcgga ctgctcgcgg
     4381 gctttgtctg gcgagcaaag ctgatgtcgc aggagcgctg gccatatcgc ctggggcctg
     4441 tcatcggggg tatcgccctg ctggccttta ccggtaccgg cgatgaaaac accgatatag
     4501 gcgcgcacct ggccggcttc gtctgcggct tcgcgaccgg cactgcgctg accctggcgg
     4561 cacagcagtt ggccaatccg cggctgcaga agctatgcgg tgctgccgcg ctcatgctcg
     4621 tgctcgcagc ctggttgaaa gcgctcactt gggaagcgtg aggagccggg cattgaaccc
     4681 tgtacggatg tacagtattc tccggccttc cactggagga gatcaggcca tggctctgca
     4741 caaaggccgc ggcgcccttt cccgcaccgc cgggcggttc gagacccgct cgatagaagc
     4801 aacggaaccg gctgacgatg tcgccccgga aacggtgctg acggcaatgg aagcccgccg
     4861 catcatcagc agcaacgact cgcccgatgt gccgttcgag cgctccatca acccatatat
     4921 gggctgcgaa cacggctgta tctactgtta tgccaggccg agccacagct acctcgatct
     4981 ctcgccaggc atcgacttcg agaccaggat tttctacaag ccgaacgccg tgaaagcgct
     5041 gctggcggag tggcagaaac cgggctacgt ctgcaagccg atcacgatcg gcgcgaatac
     5101 cgatccctac cagccggccg agaagaaact ggaactgaca cggcaactcc tgcagcaatt
     5161 cctggcgcac cggcaccctg tgagcctgat cagcaagagc gggctgatgc gccgcgacct
     5221 cgacctgctt gccgagcttg cgcacctcaa gctctgctcc gtcgccatca gcgtgccgac
     5281 agcggacaac gccctcaagc ggcggctgga gccgcgcgtg ccatctgcgg acctgcgttt
     5341 ccggctcatc gaagccctgt cgtcacatgg ggtccccacc agcctgctga tggcgccaat
     5401 cataccggcc gtcaacgatc gggagatcga agacatcgtc tccagggcgg ccgcagcagg
     5461 cgcgcggcgc gcgtccttcg tgctcctgcg cctgccgttc gaactgaagc agattttttc
     5521 cgagtggctg gagggccaca tgccggaccg ggcctcccat gtcatgagcc tggtccggca
     5581 ggcgagcgga ggcaaggaat atgacaaccg cttcggcatt cgccagtccg gcagcggcgc
     5641 gtatgctgat atgattgccc agagattcag ggtcgcctgt acgaagtcgg gcatacccaa
     5701 ccagaggagc ctggatgagc tcgactgcag cagtttcgaa cctcccgggc aggggcagat
     5761 gaccctggtc ctctgagaac acaacggtcc cagaactaat atccggggcg gcagtcataa
     5821 ccgaaatcat gcatggctga tgcatcacga accatgaaag cgacgaaatt ctcgagtccg
     5881 ttcggactgg gcgcagctct gttcataatg gctgcctgcg ggttggccgg tgcccaggaa
     5941 cccccgccag aaacagagga actggaagag ataacgctcg acaggtctcc gggaggtact
     6001 ccggatgcag tttcagagca ggtctctgaa gaggaagcgc cggaggagcg tctgcagcgg
     6061 tacttcatgc tctacaagga ggcgcttgag agcaaggctt acgccgaagc ggataccctg
     6121 gcgaaacagg tagtggaact gtcaatcgga ttgttcggca ttaacagcct cgattcggcc
     6181 cgcgcgctca cgaacctcgg gatcgcacaa caccagaacg aggattacga ctccgccatc
     6241 cttaactaca ccgccgcaat cgacatcatc gaaagaatcg aagaccggct gaacggcaac
     6301 ctgatcaacc cgctgaaggg ccttggcgca gcgcagctgg cggcagggcg gccggacgaa
     6361 gctgtcagca gcttcgagcg agcggtgcac gtaagtcatg tcaacgaagg cccgcataat
     6421 ctgatgcagg tcgacgtcct ggagtcgctg gcggaaactt acctgtccgt cggtgaattg
     6481 gacgaagtga aggacatcca ggagcacatc ttcagcctcc aggcgcggga cgtggaactc
     6541 gacagcctcg aagccatccc ggcactggag cggcaggcgc gctggcagca tcggctgcaa
     6601 ctttacgaac aggagcgtta tacctggcgg aagatcatag acatcatcga ggatcgccgg
     6661 agtgacgaag atctgagcct gataccggcg ttgactgaac tcggcaagtc gtatcttttc
     6721 atcgggcagc caaacatggc attccaccag ccgacctcga tcagctcggg cgagatctac
     6781 ctgaagcgtg cgctgcggat cgcggaggaa aatccggatg cgaactggga gacgctggaa
     6841 ggagcgatgc tcgcgcttgg cgacttctat atcctgtccg gcaagccgag ccgcgcccgc
     6901 aagatataca acgagacctg gcaactcctt tcggcagatg aggaaagatt gaagaaccgc
     6961 caggcccatc tcgag
//
LOCUS       DASUOM010000100         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1013909,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000100 DASUOM010000000
VERSION     DASUOM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013909"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFY27_02040"
     CDS             complement(<1..515)
                     /locus_tag="VFY27_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX5764321.1"
                     /translation="MKPSRIVRQSTTLPKKSAADERERLTLVSKYQPAGDQSAAIAGL
                     VEGLADGLARQTLLGVTGSGKTFTMASVIQEVQRPAIILAPNKTLAAQLYGEMREYFP
                     RNGVEYFVSYYDYYQPEAYVPSSDTYIEKDASINEHIEQMRLSATKALIERGDAIIVA
                     TVSAIYGLGDP"
     gene            555..1712
                     /locus_tag="VFY27_02045"
     CDS             555..1712
                     /locus_tag="VFY27_02045"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5764322.1"
                     /translation="MARVRPSPTSAVVALAAELRAAGRSIISLGAGEPDFPTPRHIRE
                     AGKKAIDDGETRYTPIDGTMDLKLAIKAKFARDNSLHYEPAQILVSSGAKQTCFNACQ
                     AILGSGDEAIVPAPYWVSYPDMVRLAEAEPVIIDTDIDSGFRISPEQLEAAITPKTRF
                     LFLNSPSNPTGACYSRQQLVELGEVLKAHPDIVIASDEIYEHIYWAEKPFCSFAEACP
                     DLYDRTVTINGVSKCYAMTGWRIGYAGGPPPVISAMKTIQSQSTSNPCSISQVAATAA
                     LNGDQACVKQMRDAYKERHAYIVAALNEIPGFECRETDGAFYAFPRVTGALEKLGYEN
                     DPELVSLLLGSADVAMVPGSAFGAPGYVRISFACSLDTLREAVTRIKRALT"
     gene            1825..1900
                     /locus_tag="VFY27_02050"
     tRNA            1825..1900
                     /locus_tag="VFY27_02050"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1858..1860,aa:Asn,seq:gtt)
     gene            2100..2222
                     /locus_tag="VFY27_02055"
     CDS             2100..2222
                     /locus_tag="VFY27_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764323.1"
                     /translation="MQNALAKSLKIFAYVVLLLMVVGMVYASYISLRYFNGIGV"
     gene            2241..>2543
                     /locus_tag="VFY27_02060"
     CDS             2241..>2543
                     /locus_tag="VFY27_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5764324.1"
                     /translation="MNKRQSRHFAIACTGMAALAFLVLTADSHRQFPELTNAENITPE
                     VIHGKDVWHDNNCINCHTLFGEGAYYAPDLTKITKLRGEAYLTAYMKNPADFYDEDT"
BASE COUNT          535 a          762 c          731 g          515 t
ORIGIN      
        1 tccgggtcgc ccagcccgta aatcgccgat accgttgcca cgatgatggc gtcaccgcgc
       61 tcaatgagcg ccttggtagc ggacagtcgc atctgctcga tgtgctcgtt aatcgaggcg
      121 tctttctcta tatatgtatc ggagctcggt acgtaagctt ccggctggta gtagtcgtag
      181 taggagacga agtactcgac gccgttgcgc gggaagtatt cgcgcatctc accgtaaagc
      241 tgggccgcca gcgtcttgtt cggggcaaga ataatggccg gccgctgcac ctcctggatg
      301 acgctcgcca tcgtgaaggt cttgcccgag cctgtcacgc cgagcagtgt ctgccgcgcc
      361 aggccgtccg ccagtccttc caccaggcct gcgatggcgg cggactggtc gcccgccggc
      421 tggtatttcg acacgagcgt cagacgctcg cgttcgtcgg ccgcgctctt cttgggcagc
      481 gttgtcgatt gacgtactat gcgcgacggt ttcatggagt ttatctggtg agcagtgtag
      541 cagtttcaaa acgtatggcg cgggtaaggc cctcgccgac cagtgctgtc gttgcactcg
      601 ccgcggagct gcgtgcagct gggcggagta tcataagtct cggtgcgggc gaaccggatt
      661 tcccaacgcc ccggcacatc cgcgaagcgg gcaagaaggc gatcgatgac ggcgaaaccc
      721 gctatacgcc aatcgacggc acgatggacc tcaagctcgc aatcaaggcg aagttcgccc
      781 gcgacaactc gcttcattac gagccggcac aaatcctggt atcgagtggc gcgaagcaaa
      841 cgtgcttcaa cgcatgccag gcaattctcg ggtccggcga cgaagccatc gtaccggcgc
      901 cttactgggt atcgtatccg gacatggtca ggctggcgga agccgaaccg gtcattatcg
      961 acaccgatat cgacagcggg ttccgcattt ctcccgagca gctcgaagca gccatcacgc
     1021 cgaagacgcg cttcctgttt ctcaacagcc cgtccaaccc gaccggcgcc tgctactcgc
     1081 ggcaacagct cgtggaactg ggcgaggtcc tgaaagcaca tcccgatatc gtcattgcga
     1141 gtgatgaaat ttacgagcat atctactggg cggaaaaacc cttctgcagc ttcgcggaag
     1201 cgtgcccgga cctgtacgac cgcacggtga ccatcaacgg ggtctcgaaa tgctatgcga
     1261 tgaccggctg gcggattggc tacgccggtg gcccccctcc ggtcatctcg gcgatgaaga
     1321 cgatccaaag ccaaagcacc tcgaatccct gcagcatttc acaggttgct gccaccgctg
     1381 cattgaacgg cgaccaggcc tgcgtaaaac agatgcggga cgcctacaag gaaaggcacg
     1441 cttacatcgt cgctgcgctc aatgaaatcc ccggcttcga gtgtcgcgaa acggacggtg
     1501 ccttttatgc atttccccgg gtcacgggcg ccctcgagaa gctgggatat gaaaatgatc
     1561 cggaactggt gtcactcctg cttggcagtg cggacgtcgc catggtgccc ggcagcgcat
     1621 tcggcgcccc aggctacgtc aggatctcgt ttgcctgctc gctggacacg ttgcgcgaag
     1681 cggtgacaag gataaaaaga gcgctgacct gacgagaagc cgtgcggttg taactgctgt
     1741 gtttacgcgg gtttgcttcc cttgacttga tttccgggct ggaccagaat gcgacccctg
     1801 ccaaggatcg caccctcaca ccattccccg gtagctcagt tggtagagcg attgactgtt
     1861 aatcaattgg tcgctggttc gagtccggcc cggggagcca tcttctgatt gcttgctgcc
     1921 gattgcccga agccgaatgc gggcaattgc agcaaagcca atgaacgaag cttgaccagg
     1981 ttcagcgacc ctgcgtttct cgcctggtcg catttcactc cgtgcggtct ggagaccaac
     2041 gatcaaaaat ggcagacttg gccggtgccg tcctggcgcg tctacgaaaa ggagcagtca
     2101 tgcagaatgc tctcgctaaa tccttaaaga ttttcgccta tgtcgtcctc ctgctaatgg
     2161 tcgtcggcat ggtgtatgcg agctacatca gcctccggta cttcaacggc atcggcgttt
     2221 aacctcgagg ggactcaata atgaacaaga gacagtcccg ccatttcgct atcgcctgca
     2281 cgggcatggc cgcactggct ttcctggtac tgacggcaga cagccaccgg cagttccccg
     2341 agctgacgaa cgccgagaac atcactccgg aggtcataca tggcaaggat gtatggcacg
     2401 acaacaactg cattaactgc cacacgctat tcggtgaggg cgcctactac gcacccgatc
     2461 tcacgaagat cacgaaactg cgcggcgaag catacctgac cgcatacatg aaaaatccgg
     2521 cggacttcta tgacgaagat aca
//
LOCUS       DASUOM010000101         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1108251,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000101 DASUOM010000000
VERSION     DASUOM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108251"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /gene="cysK"
                     /locus_tag="VFY27_02065"
     CDS             complement(<1..1410)
                     /gene="cysK"
                     /locus_tag="VFY27_02065"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5764325.1"
                     /translation="MARYENILETIGNTPLVRLQKLAPPGVNLFVKVESFNPMGSVKD
                     RMAREMIETAERSGELKPGQTVVEATSGNTGIGLAMVCAQKGYPLVVTMAESFSIERR
                     KMLRFLGAKVVLTPAAEKGSGMLKKAVELAQRHGWFLCRQFENQANAAAHMRTTAREI
                     LDDFDNEPLHYFVTGFGTGGTLLGVSRVLRKESPGTRIIVGEPDNSQLLGSGIPQPRG
                     ADGAPSASHPNFRPHLMQGWGPDFIPPLTEEAVANDLFDEVLPVAGDDALRLSRELAR
                     REGIFVGTSSGATLAAALAVAGRAPAGANIVCMLPDTGERYLSTPLFDDIGVDMTEEE
                     LAVSRSTPSCRFDVPAAAAPPAPAPAEIDAEAVRFVDRVLTDEPVVLFALEWCEFCWS
                     VRKLFGKLGVEYRSVDLDSVEYQEGDRGGKIRAVLSARLGVSTIPQVFVGGEHIGGCT
                     EVFDEMRSGELQERLRERGI"
     gene            1812..>3961
                     /locus_tag="VFY27_02070"
     CDS             1812..>3961
                     /locus_tag="VFY27_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5764326.1"
                     /translation="MSLRFLGEFGVIRDGEAMALPPSRKTRALLAYLCLNRRRFRREQ
                     LCDLLWEVPDDPRGSLRWSLSKLRRLLDDEDRQRVLADRTSVEVDVRDVDIDVVALHA
                     LAGDGLKDAPVETLEQAARRYRGNFLEELEFFNFHDFHAWCVAEREQASRAQAAVLRQ
                     LVDRLQETPARALPYARSLVGISPYDESSRAILIRLLHESQHLDEASQQYQIGLRMLK
                     EAGVVSTGALLRARVGGKSGSTVAPAELPGQHARYDAGVRPVTDSLVGRDEEVAILAD
                     AMSRVASDALAQIIVVRGEPGIGKSRMLQCVSRLATDRNALLLEASAFQPESIRPFAL
                     WIDALRALDPEVAERLFGGSEAQNRDRLLSGLSAFITAESNARPVVIVFDDVQWCDES
                     SAAALHYVARMNRRQPLLAVLAVRDGELQDNAVLQQTLGGLRHDGLLREIKLFPLSAA
                     ATAQLVRSCCPEIDAERLHKACRGNPLLAIELARAQRDGAGASSFDQLVQERLTRFDV
                     NGAEVLKWAAVLSPRIDVPMLVTLTSFDAMEVTAVLESAEQRAILSATAHGLRFSHEL
                     IARAIYHGISPLRRQVMHRRVAELLEQNTAMDLARASDLAHHATQSGDPALAAKAMVS
                     AGRLCLRFFANAEALSLARKGLQFSAQLPAADRVCLKIDFHDIMLAAAPLSDWEEAAR
                     RYVELAEEALDHGKLAHARLGYHMASSVRWQHGQ"
BASE COUNT          667 a         1290 c         1277 g          727 t
ORIGIN      
        1 gattccgcgt tcccgcagcc gctcctgtaa ctcgccgctt cgcatctcgt cgaagacctc
       61 ggtacagccg ccgatgtgct cgccgcccac gaaaacctgg gggatcgtgc tgacgcccag
      121 cctcgctgac agcaccgcac gtatcttccc gccgcggtct ccttcctggt attcgacgga
      181 gtccaggtcg acactccggt attccactcc cagcttgccg aagagcttgc gcaccgacca
      241 gcagaattcg caccattcca gggcaaacag gacgactggc tcgtcagtca gaacccggtc
      301 aacgaaacgc accgcttcgg catcgatttc tgccggcgcc ggcgcaggcg gcgccgctgc
      361 ggcgggaacg tcgaagcggc aggatggagt ggagcgcgat accgcgagtt cctcctccgt
      421 catgtcgacg cctatgtcat cgaacagcgg cgtcgacagg tagcgctctc ccgtatccgg
      481 aagcatgcac acgatgttcg caccggcggg cgcgcgtccc gccacggcca gcgccgctgc
      541 cagcgtagcg ccgctcgagg tgccgacgaa aatgccttcc cgccgcgcaa gctcgcgcga
      601 cagccgcagc gcatcgtcac cggccaccgg caggacttcg tcaaagagat cgttcgctac
      661 cgcctcttcc gtcagcgggg gtatgaaatc cggaccccaa ccctgcatca ggtgcgggcg
      721 gaagttcgga tgactggcgg aaggcgcgcc atcggcgccg cgcggttgcg gtatgccgct
      781 gccgagcagc tgggaattgt ccggttcccc gacgatgatt cgggttcccg ggctttcctt
      841 tcgcagcacg cgggagacgc caagcagcgt gcctcccgtg ccgaagccgg tcacgaaata
      901 gtgcagcggc tcgttgtcga agtcgtcgag tatttcccgt gccgtggtgc gcatgtgggc
      961 ggccgcattc gcctggttct cgaactgcct gcacaggaac cagccatgcc tctgtgcaag
     1021 ctcgaccgct ttcttcagca tcccggagcc cttctccgcc gccggcgtca gcaccacctt
     1081 tgcgccgagg aagcgcagca tcttgcgtcg ctcgatgctg aagctctccg ccatcgtcac
     1141 gaccagcgga tagccctttt gcgcgcagac catggcgagg ccgataccgg tattgccgct
     1201 ggtcgcttct accaccgtct ggccgggctt cagctcaccg cttctttccg ccgtctcgat
     1261 catttccctt gccatgcggt ccttcacgga acccatcggg ttgaacgatt cgaccttgac
     1321 gaaaaggttg acgcccggcg gtgcgagctt ctgcaaccgg acgagcggcg tgttgccgat
     1381 cgtttcgaga atgttttcgt agcgagccat tgaacatctc cttcagaatg cagcgtgcgg
     1441 ttcacgaagg tgaacctccg cgggtggcgt ctcgaccggg aagaggtcct gtttcaggaa
     1501 cgccgtgaac ccatccatgg gggctctgcg gaaaacatcc tgttttccga cagtcctgaa
     1561 acaggacctc ttcccggcca agacaccact gaaccgttat cgcgtcgtga accgaccagg
     1621 cctcggaagg cctctacccg ccaaaacgct gtcgcttcga taccgtcctg ccgttagagg
     1681 taagcctcaa tcctgaccgg tgtaatcagc gtggaagcgg ccgtggtttt ttttgcccgg
     1741 tgccgatttt gcgccggatt gcgggcaact gcacccggcg gcgcggcagc ggctacactg
     1801 tagtgatggg cttgagtctt aggtttctcg gggaattcgg cgttatccgg gatggcgagg
     1861 caatggcgtt gccgccgtcc aggaagaccc gcgcgctgct cgcctacctg tgcctgaaca
     1921 ggcggcggtt ccgccgggag cagctgtgtg acctgctgtg ggaggttccg gacgacccgc
     1981 gcggttccct gcgatggagc ctgtcgaagc tgcgccggct gctggacgac gaggaccggc
     2041 agcgggtcct ggccgatcgc acgagcgtgg aagtcgatgt gcgggacgtc gatatcgacg
     2101 tggtggcgct acatgccctc gccggagatg ggctcaagga cgcgccggtc gagacgctcg
     2161 agcaggcggc gcgtcggtac cggggcaatt tcctcgaaga actggagttc ttcaattttc
     2221 acgattttca cgcctggtgc gtcgcggagc gggagcaggc ttcacgggcg caggctgccg
     2281 tgctccgcca actggtcgac cggctgcagg aaactcctgc tcgtgccctg ccttacgctc
     2341 ggtcgctggt cggcatctcg ccctatgacg aatcctcgcg ggcgatattg atccggctgc
     2401 tgcacgagtc gcagcacctg gacgaggcaa gccagcaata ccagatcggc ctcaggatgt
     2461 tgaaggaagc cggtgtcgtg tccacgggcg ccttgctccg cgcacgcgtg ggcggcaaga
     2521 gcgggagcac ggttgccccg gccgaacttc cgggacagca cgcccggtac gatgccggtg
     2581 tacggccggt gaccgactcg ctcgtcggcc gcgacgagga agtcgcaatc ctggctgacg
     2641 caatgtcgcg cgttgcctcc gatgcgcttg cgcagattat cgtggtgcgc ggcgaaccgg
     2701 gcattggcaa gagccggatg ctccagtgcg tatcgcgatt ggccacggat cgaaatgccc
     2761 tcctgctgga agcgagcgcg tttcagccgg agtcgatacg tccattcgcc ctgtggatcg
     2821 acgccctgcg cgcgctggac cccgaagtcg cagagcggct cttcggcggc agcgaggcgc
     2881 agaaccggga ccgcctcctg tccggcctga gcgccttcat caccgcggag tcgaacgcac
     2941 ggccggtggt catagttttc gatgacgtgc agtggtgcga cgagtccagt gcagcggcgc
     3001 tgcattacgt agcgcgcatg aaccggcgcc agcccctgct tgcggttctc gcggtgcgcg
     3061 acggcgagct ccaggacaac gccgtgttgc aacagacctt gggaggcctg cggcacgacg
     3121 ggcttctgcg cgagatcaag ctgttcccgt tgtcggccgc ggccaccgcg caactggttc
     3181 gaagctgttg cccggaaata gacgctgaac gcctgcacaa ggcatgccgc ggcaacccgc
     3241 tgctggcgat agagcttgcg cgcgcccagc gagacggcgc gggagcaagc tcgttcgacc
     3301 agctcgtgca ggagaggctg acgcgcttcg acgtcaacgg ggcggaagta ctgaaatggg
     3361 cggcggtctt aagcccgagg atcgacgtgc caatgctggt gacgctcaca tccttcgacg
     3421 caatggaggt gactgcggtc ctcgaaagtg ccgagcagcg tgccattctt tctgcgacgg
     3481 cgcacggtct gcggttctcg cacgagttga tcgcacgcgc catctaccac ggaatctccc
     3541 ccttgcggcg gcaggtcatg catcgccggg ttgcggagct gctggagcag aacacggcga
     3601 tggatctcgc ccgcgcctcg gacctcgcgc atcacgcgac gcaaagcggg gatcccgcgc
     3661 tggctgcgaa ggccatggtg tctgcagggc gcctgtgcct gcgctttttc gcgaacgccg
     3721 aagcgctgtc gctggcgcgc aagggcctgc aattctccgc gcaattgccc gccgcggacc
     3781 gtgtgtgcct taagatcgat ttccacgaca tcatgcttgc cgccgcgcca ctttcggact
     3841 gggaggaagc cgcgcggcgc tacgtggaac tggccgagga agcgctcgac cacggcaagc
     3901 tggcacatgc gcggctgggc taccacatgg cctcttccgt gcgctggcag cacggtcaat
     3961 g
//
LOCUS       DASUOM010000102         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_566835,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000102 DASUOM010000000
VERSION     DASUOM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566835"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFY27_02075"
     CDS             <1..946
                     /locus_tag="VFY27_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764327.1"
                     /translation="DLISGDRQLTLLQMQKVARLLNVEPKFIPLRQPLTGRSVETQII
                     EKMIRRYGINYVASRAVTLFDIVGFGLLTPFEQMTQLNSLSYSLNSAHAKMMEKEVGI
                     NFARSSSGDGFYVWNRDHGLEANVNLYHFMHIVLADNAIARSKATSNAVPKLRACFHV
                     GSCYEFHQAEGLNPTIHDYIVGDVTIELARMIEAALPGQILVGDFLAKLSTDLLGHED
                     SQVHLDAIAFLQRAQGNLSKLSGLELSGERVSAIKCYLTGERLESGEFNIRKLQIRDK
                     HGLSRAAYNAKVNIYRVLAQPILLGIEDKKLGQILEPA"
     gene            complement(965..1041)
                     /locus_tag="VFY27_02080"
     tRNA            complement(965..1041)
                     /locus_tag="VFY27_02080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1005..1007),aa:Pro,seq:ggg)
     gene            complement(1118..1474)
                     /locus_tag="VFY27_02085"
     CDS             complement(1118..1474)
                     /locus_tag="VFY27_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5764328.1"
                     /translation="MLEPGNNDELPPIPGKRYFTIGEVSDLCAVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVLIIRQIRSLLYDDGFTIGGARQRLTGDEAKTDVSQSQQII
                     KQLRLELEQVLKILRR"
     gene            complement(1455..1754)
                     /gene="ihfA"
                     /locus_tag="VFY27_02090"
     CDS             complement(1455..1754)
                     /gene="ihfA"
                     /locus_tag="VFY27_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX5764329.1"
                     /translation="MALTKADMAESLFDELGLNKREARELVDLYFEELRASLAVGEQI
                     KLSGFGNFDLRDKNQRPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGTGE"
     gene            complement(1791..>3791)
                     /gene="pheT"
                     /locus_tag="VFY27_02095"
     CDS             complement(1791..>3791)
                     /gene="pheT"
                     /locus_tag="VFY27_02095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5764330.1"
                     /translation="LGDDSDGILSLPSDASPGTPLARYLGLPDAVYEVNLTPNRGDCF
                     SILGMAREISALTGAPLAQPALAAVEQSSDAEYPVALEDPRACPRFAGRVVRGIDTAA
                     RTPLWMTERLRRCGVRAIHPVVDVTNYVMLELGQPLHAYDLDRLAGTIRPRFAKAGER
                     LTLLDEREVELSPDTVVVTDDSGPVGLAGIMGGLPTAVTEATMNVFLEAAFWRQDVIA
                     GRARAYGLHTDASLRYERGVDPEGQARAIERATNLLLEIAGGSAGPLTDIVHADHLPK
                     RGPVALRRSRLHSLLGLQLNDAEVSRILRSLRLAVQETGGGWTVQPPGFRFDIAIEAD
                     LIEEVARVYGYDRIPEATATAALPLRPVTERRIDLERVADTLVARDYQEVITYSFIDA
                     ASDQVVSCVESNLTLTNPISSEMSVMRASLWPGMLKIAASNLARQQDRVRLFEIGKTF
                     HGTLEDAKEVVRVAGLATGTAAPEQWGVIPKEVDFFDLKSDLEALFDLAGAGGEVSFM
                     EARHPALQAGQSAQVLRDGKAIGVAGKLHPSASRALDISRSVVLFELDAAAAFAARVP
                     VATAVSRFPAIRRDIAVIVKDDITSAQLIQVALSAAPDIIRRAVVFDVYRGPGIEAGL
                     KSVALGLILQETSRTLTDVDADTAMSKAVQELQQECSATLRE"
BASE COUNT          774 a         1155 c         1092 g          770 t
ORIGIN      
        1 ggacctgatc tcgggcgacc gccagctgac gctgctgcag atgcagaagg tggcccgcct
       61 cctcaacgtt gaaccgaagt tcatcccgtt acgccagcct ttgaccggcc gctcggtcga
      121 gacgcagatt atcgaaaaga tgatccggcg ctacggcatc aactacgtgg ccagtcgagc
      181 ggtaaccctg ttcgacatcg tcggcttcgg gctcctgacg ccgttcgaac aaatgaccca
      241 actgaacagc ctctcctact cgctcaattc cgcgcacgcg aaaatgatgg agaaggaagt
      301 cggcatcaac ttcgcaaggt cctcgagcgg tgacggcttt tacgtatgga accgcgatca
      361 cgggctcgag gcgaacgtca atttatacca tttcatgcat atcgtacttg ccgataatgc
      421 gatcgcccgc agcaaagcaa cgtcgaatgc cgtaccaaaa ttgcgtgcct gcttccacgt
      481 cggcagttgc tacgagtttc accaggcaga ggggctgaat ccgacgattc atgactacat
      541 cgtcggcgat gtaacgatcg agctcgcacg catgatcgag gctgcactcc ccggccagat
      601 cctggtgggg gacttcctgg cgaagctgtc gaccgacctg ctcggacacg aggattcaca
      661 ggttcacctc gatgccattg cgttcctcca aagggcacag ggaaaccttt cgaagctgag
      721 tggactcgag ttatcgggcg agagggtttc cgccatcaag tgttacctga caggcgagcg
      781 cctggagagc ggcgagttca atatccgaaa actgcagatc cgcgacaagc atggtctttc
      841 gcgcgcggcg tacaacgcga aggtaaacat ctatcgtgtg ctggcgcagc cgatactgct
      901 gggcatcgag gacaagaagc tcgggcagat actggaaccc gcctgacgtc tcgcgatttt
      961 taaatggtcg gggcggggag attcgaactc ccgaccccca caaccccatt gtggtgcgct
     1021 accaggctgc gctacgcccc gaccgggtaa aaaaagcttg agggcggcca tgatactggg
     1081 cgcccttttc agttgaaagg cagttgataa ggtcgctcta ccgccgcagt atcttcagaa
     1141 cttgctcaag ttccaatcgt aactgcttga taatttgctg actctggctg acatcggtct
     1201 tagcttcgtc gccggtgagg cgctggcgcg caccgccgat ggtgaaaccg tcgtcgtaca
     1261 gaagactgcg aatctgacgg ataatcagca catcctgacg ctggtagtaa cggcgattgc
     1321 ctcgccgctt taccggtttc agttgtggga attcctgctc ccagtaacga agtacgtgcg
     1381 gcttgacagc gcacaagtcg ctgacttccc cgatcgtgaa atatcgttta cccggaatag
     1441 gaggtagttc gtcgttattc cccggttcca gcataggcct ctactcgggc ttttagtttt
     1501 tgtcccggtc ggaaagtgac aacacggcga gccgtgattg gaatttcctc tccagttttt
     1561 ggatttctgc ccggacgttg gtttttatcc cgaagatcaa aatttccaaa acccgaaagc
     1621 ttgatctgct cacctaccgc aagtgatgcg cgcagctctt caaaatacaa atcaacgagc
     1681 tcgcgtgctt cgcgcttgtt caatcccagt tcatcgaaaa gggattctgc catgtctgct
     1741 ttcgtcagtg ccattcgtct tggttctcga ctgattcgaa tgtgttggtg tcattctctt
     1801 aacgtcgcgg agcattcttg ttgcagttct tgcaccgctt tcgacattgc ggtgtccgcg
     1861 tcgacgtcag taagcgtgcg cgaagtttcc tgcaaaatca agcctaaagc tacgcttttt
     1921 agcccagctt ctataccagg cccgcgatac acgtcgaaaa ccacagcacg gcggattata
     1981 tccggagccg ccgataacgc cacctgtatc agttgcgccg acgttatgtc gtctttcacg
     2041 atgaccgcaa tgtcacgccg gatggcggga aaccgggaaa ccgccgttgc aacaggcacg
     2101 cgggcggcaa acgctgctgc ggcgtccagc tcgaaaagca ctacggagcg gcttatatcc
     2161 agtgcgcgcg aagcggatgg gtgcagtttc cccgccacgc cgatcgcctt gccgtcgcgc
     2221 aaaacctgcg cggattggcc cgcctgcagg gccggatggc gggcttccat gaagctcacc
     2281 tcgcctcccg cacccgccag gtcgaacagt gcctcgaggt ccgacttgag gtcgaagaaa
     2341 tcgacctctt tcggaatgac gccccattgt tcgggcgcgg ccgttccggt ggccaggcct
     2401 gctactcgca cgacctcttt ggcgtcctcg agtgttccat gaaacgtctt gccgatctcg
     2461 aagagccgca ccctatcctg ctgacgcgcg aggttcgatg cggctatctt cagcatgccg
     2521 ggccacaacg acgcgcgcat caccgacatc tcggaagaga ttggattagt caaggtaagg
     2581 ttcgactcta cacaactaac cacctggtcg gacgctgcat cgataaagct gtacgttatg
     2641 acttcctggt aatcgcgtgc gacaagtgtg tcggcgacgc gctcgagatc gatgcgccgc
     2701 tccgtcacgg gcctcagtgg cagggcggcg gttgccgtcg cttccggtat cctgtcgtaa
     2761 ccgtaaaccc gcgccacctc ctcgatgagg tctgcttcga tggcgatgtc gaagcggaaa
     2821 ccgggcggct gcaccgtcca gccaccccct gtttcttgca ctgccaggcg caggcttcgg
     2881 agaatgcggc ttacttccgc atcattgagc tgcaagccga gcagggaatg cagccgtgat
     2941 cggcgcaacg cgaccgggcc gcgtttcggc aggtgatccg catgcacaat gtccgtcaga
     3001 ggcccggcgg aaccgccggc aatctccaga aggaggttgg tcgcccgctc gatggcccgc
     3061 gcctggccct ccggatccac accccgctcg taccggaggg aagcgtcggt atgcaggcca
     3121 taggcccgcg cccggccggc gatgacatcc tggcgccaga acgccgcctc caggaaaaca
     3181 ttcatggtcg cctccgtgac cgccgtagga agcccgccca tgatccccgc aagtccgaca
     3241 gggccggagt catcggtcac aacaaccgtg tcgggagaaa gctccacttc ccgctcatcg
     3301 agcagtgtga gccgctcgcc cgctttcgca aaacgcggac ggatggttcc ggcaaggcgg
     3361 tcaagatcgt aggcgtgcaa tggttgcccc agttcgagca tcacatagtt cgtaacgtcg
     3421 accacgggat ggatcgcgcg aacgccgcac ctgcgcagcc gctcagtcat ccacaacggc
     3481 gtgcgggctg cagtatcgat gccgcggacg actcggcccg caaagcgggg acatgctcga
     3541 gggtcctcca gcgcgaccgg atattctgcg tcggacgatt gctccacggc agccagggcg
     3601 ggctgtgcga gcggggcgcc ggtgagcgct gatatctcac gcgccatgcc aagtatgctg
     3661 aaacagtctc cccggtttgg tgtcaggttc acctcgtaca cagcgtccgg aagccccagg
     3721 tagcgggcca gcggcgtgcc gggagacgca tcggacggca ggctcaggat gccgtcggaa
     3781 tcgtccccga g
//
LOCUS       DASUOM010000103         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1479973,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000103 DASUOM010000000
VERSION     DASUOM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479973"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFY27_02100"
     CDS             complement(<1..378)
                     /locus_tag="VFY27_02100"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352730.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5764331.1"
                     /translation="MRSLLITNARLVNEGNVSDADVLIRGERIEKIARSINAPDVARI
                     LDAAGRWLLPGMIDDQVHFREPGLTHKGDMASESAAAVAGGITSFMDMPNVSPQTTTR
                     EALADKYAAAKGRARANYAFYLGG"
     gene            complement(382..1344)
                     /gene="pip"
                     /locus_tag="VFY27_02105"
     CDS             complement(382..1344)
                     /gene="pip"
                     /locus_tag="VFY27_02105"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX5764332.1"
                     /translation="MNDLSERRTLYPPIEAYDTGELDVGDGHILYYEQSGNPEGKPVV
                     FLHGGPGGGCTPKMRQFFDPSRYRIVLFDQRGSGKSRPHAHLEANTTWHLVSDIERLR
                     ERLDIGRWQVFGGSWGSTLALAYAQTHPERVKELVLRGIFLLRKKEIDWFYQFGASEI
                     FPDRWQGFLEPIPEDERDDLLHAYHKYLTNDDPDVRLRAARAWSVWEGSSCMLIPSPE
                     VEKVFSAEEMALAMARIECHYFVNEGFMRENQLLKNIDIIRHIPAVIVQGQYDIVCPA
                     VSAYELSRAWPEAVLHIVPDAGHAAFEAGNVHELVTTTDRFAGI"
     gene            1596..>2348
                     /locus_tag="VFY27_02110"
     CDS             1596..>2348
                     /locus_tag="VFY27_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5764333.1"
                     /translation="MKFSQVVLAFSGAVVSLLLALSATAADVDISFTEYTLDNGLRLI
                     VHEDTKAPIVAVNIWYHVGSKNEQPGKTGFAHLFEHLMFNGSENFNEEIRQPLERAGV
                     TGMNGTTYFDRTNYFETVPKNVLDMTLWLESDRMGHLLGVVDEAKLTQERGVVQNEKR
                     QSENQPYGQAFNHILRNVFPPQHPYSWSPIGSMEDLDAASLEDVHEWFKTYYGPNNAV
                     IVIAGDIRPEEALERVEHYFGDIPPGPPIAKYE"
BASE COUNT          474 a          713 c          706 g          455 t
ORIGIN      
        1 gccgccgagg tagaacgcgt agttggcacg ggcacggccc tttgcagccg cgtacttatc
       61 ggcaagcgcc tcacgcgtcg tggtctgcgg actgacgttc ggcatgtcca tgaagctggt
      121 gataccgcct gcgacggcgg ccgcagactc gcttgccata tcgcccttgt gcgtcagtcc
      181 aggttcgcga aaatgcacct ggtcgtcgat cattccgggc aggagccagc ggcctgcggc
      241 gtcgaggatc cgggcgacgt ccggggcgtt gatgcttcgt gcgattttct caatgcgctc
      301 gccgcggatc agtacatcgg cgtcgctgac attaccttcg ttaacaaggc gcgcgttcgt
      361 aatcagcaag cttctcaata atcaaatccc ggcaaaacga tcggtggtgg tgacgagctc
      421 atggacgtta ccggcttcga atgcggcatg tcctgcgtcg ggcactatat gaagtaccgc
      481 ttccggccac gcgcgcgaca gctcgtatgc agagaccgcg ggacagacga tgtcgtactg
      541 tccctggacg atcactgcgg gaatgtgacg aattatatca atgtttttca gcagctggtt
      601 ctctcgcatg aagccttcgt tgacgaagta atgacactcg atacgcgcca tcgccagtgc
      661 catttcttcg gcggagaaaa ccttctctac ttccggagac ggtatcagca tgcacgatga
      721 cccttcccag acggaccagg cgcgagcggc ccgcagtcgt acgtcggggt cgtcgttcgt
      781 caggtacttg tggtacgcgt gcagcaggtc gtcccgctca tcctccggaa taggctcgag
      841 aaaaccctgc catcggtcgg gaaagatttc gctggcgccg aactggtaaa accagtcaat
      901 ttccttcttc cgcagcagga aaatcccgcg caggacgagt tccttcaccc gttcgggatg
      961 cgtttgggcg taggccagcg ccagcgtgct gccccaggag ccgccgaaga cctgccagcg
     1021 cccgatgtcc aggcgctctc gcagccgttc aatgtcgctg acgagatgcc atgtcgtgtt
     1081 cgcttccagg tgggcgtgcg ggcggctctt gccgctaccg cgctggtcga acagcacgat
     1141 ccggtagcgg ctcgggtcga agaactgccg cattttcggc gtgcagccgc cgccagggcc
     1201 gccgtgcagg aatacgaccg gcttaccttc cgggttaccg gactgttcgt aatagagaat
     1261 atgcccatcg ccgacgtcca gctcgccggt gtcgtaagcc tcgatgggcg gataaagggt
     1321 gcgtcgctcg gagaggtcat tcatgcgcgc attatgggag ggcagcgcga agattgcagc
     1381 aaatgccacg tgacggccgt cggagcaccg gaacctcttt tccctgagac agtccttgat
     1441 agcaggagcc cgccatgcgg gcgtgtgcgc tacgcctcag cgaactcgcc cgcatggcgg
     1501 gctcctacag gtggagatta cgtcggcacg cagatcgttc cgatacacta gagtgccttc
     1561 gaagtgccga aagactcctg ctcacaaggt cctgtatgaa attctcccaa gttgtgttgg
     1621 cgttctccgg cgctgtcgtt tcgctgctgc tcgccctcag cgcaaccgcc gcggatgtcg
     1681 acatctcgtt caccgagtac accctggaca acgggctgcg cctgatcgtg cacgaggaca
     1741 caaaggcgcc gatcgtggcg gtcaacatct ggtaccacgt cggctccaag aacgagcagc
     1801 ccgggaagac cggctttgcg cacctgttcg agcacctgat gttcaacggc agtgaaaatt
     1861 tcaacgagga gattcgacag cccctcgagc gtgccggcgt cactggcatg aacggcacca
     1921 cgtacttcga ccgcacgaac tacttcgaga cggtgccgaa gaacgtgctg gacatgacac
     1981 tgtggctcga gtcggaccgc atggggcatc tgctgggtgt cgtagacgaa gccaagctta
     2041 cgcaggagcg cggcgtagtg cagaacgaga aacgccagag cgagaaccag ccgtacggcc
     2101 aggccttcaa ccatatcctg agaaacgtct ttccgccgca gcatccgtat tcatggtcac
     2161 caataggctc catggaagac ctggacgccg ccagcctgga agacgtccac gaatggttca
     2221 aaacgtacta cggccccaac aatgcggtga tcgtcattgc cggcgacatc cgcccggagg
     2281 aagcgctcga aagggtcgag cactacttcg gcgatatccc gccggggccg ccgatcgcaa
     2341 agtacgaa
//
LOCUS       DASUOM010000104         4550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_364516,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000104 DASUOM010000000
VERSION     DASUOM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364516"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..803)
                     /locus_tag="VFY27_02115"
     CDS             complement(156..803)
                     /locus_tag="VFY27_02115"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5764334.1"
                     /translation="MRMILFGAPGSGKGTQAKNLMRDRNIPQISTGDMLRTAAASGSG
                     LGKQVKSIMDSGQLVSDDIMLDIIKERLEEPDTVNGFVLDGFPRTLAQAEGLEKLLDR
                     MGKPLDVAVLMDIDFDILLKRLTGRRTCSATGKLLNIWFSPKEEIEECRTQGGELIQR
                     EDDNEETISDRLDVYRKHTEPVIDYYKSRGKLTTIDAEGSVEEVYARLLDVLQET"
     gene            1011..2273
                     /locus_tag="VFY27_02120"
     CDS             1011..2273
                     /locus_tag="VFY27_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5764335.1"
                     /translation="MAKMNAFYAQSGGVTAVINATACGVIETARENKSRIGNVYAGRN
                     GIIGALKEDMIDTGKETARTIAALRHTPGGAFGSARYKLKGIEENRAQYERLVEVFRA
                     HDIGYFFYNGGNDSMDTAHKVSLVAKRLGYPVRCLGIPKTVDNDLPVTDTCPGFGSVA
                     KYVAVSTREAALDVLSMAETSTKVFILEVMGRHAGWIAAAGGLAGEKPEEAPHIILFP
                     EIPFEKDVFLKRVRECVESFGYCVIVVSEGARYEDGRFLAEAGTRDAFGHAQLGGVAP
                     VVANMVRENLGYKFHYAVADYLQRSARHIASAVDVEQAYAVGKAAVKMALAGKTAVMP
                     VIKRVSDSPYRWRIEEAALSRVANKEKKLPRRFISADGFGITAAARRYLSPLIRGEDY
                     PPYINGLPRYVTLRNEPVPKRLNTDFVV"
     gene            2441..4483
                     /locus_tag="VFY27_02125"
     CDS             2441..4483
                     /locus_tag="VFY27_02125"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5764336.1"
                     /translation="MFNEFALWIAIGAGVLAVLYGIFSAQWILKQPAGNERMQVIAQA
                     IQEGAGAYMNRQYLTIGMVGIVLFIVLVVALGWTTGIGFAIGAIFSGLAGYIGMFVSV
                     RANVRTAEAARHGVNPALNIAFRGGAITGLLVVGLGLLGVAGYYAVLGGMGVSTDDAI
                     HALVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPGVIADNVG
                     DNVGDCAGMAADLFETYAVTIIATMLLGSLIADSFGSAAITYPLVLGAVSIIASIIGT
                     FFVRTSDGGKIMNALYKGLIVSGALAAVAFWFVTNSMMGDTGNAGGIFGSALIGLALT
                     AALVVITEYYTGTEYKPVKTIANASLTGDATNIIAGLGISMKATALPVLAVCLSIWGA
                     FALAPEGDSLAGLYNIAIAATAMLSMTGVIVALDAFGPITDNAGGIAEMSDLPSDVRN
                     ITDQLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTNALESANIIVDFDLSDHLVIIG
                     LFIGGLVPYLFGSMAMEAVGRAAGAVVNEVRRQFREIPGIMEGTGKPDYGRAVDLLTK
                     AAIKEMIIPSLLPILVPLAVGLLLGPKALGGVLLGTIITGLFVAISMTAGGGAWDNAK
                     KYIEDGNCGGKGSDAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLCVPLLG"
BASE COUNT          874 a         1394 c         1391 g          891 t
ORIGIN      
        1 atcgcttcca gaaaggaact gacggccgca ctctatggcg cgcggaaact gcgggtgttc
       61 aggggctggc ggcaggagct ggtgggtgag aggctcctgg cgatgctcgg gtagctcgag
      121 gtttcggtcg cccgtgaggg cgaccaatgt gtcggctagg tttcctgcaa tacatcaagc
      181 aggcgcgcat agacttcctc gacgctgcct tcggcgtcga tcgttgtcag cttgccgcgc
      241 gacttgtaat agtcgattac cggttccgta tgctttctat atacatcgag gcgatcggag
      301 atcgtttcct cgttgtcgtc ctcacgctga atgagctcgc cgccctgcgt tcggcattcc
      361 tcgatttctt ccttcgggga aaaccagatg ttgagcagct ttcccgtggc cgagcaggta
      421 cggcggccgg tgagccgttt cagcaggatg tcgaagtcta tgtccatcag cacggccaca
      481 tccagcggct tgcccatgcg gtcgagaagc ttctctagtc cttccgcttg cgccagtgtc
      541 cttggaaacc cgtcgagaac gaagccattg acagtatccg gctcctcaag tcgctctttt
      601 attatgtcga gcatgatgtc gtccgacacg agctggccgg aatccatgat ggatttcacc
      661 tgcttgccca gcccgctgcc cgaggccgcg gcggtgcgca acatgtcccc ggtcgaaatc
      721 tggggaatgt tccggtcccg catcaggttt ttcgcctggg taccctttcc cgacccgggc
      781 gcgccgaaca agatcatgcg catatccact tccttatctg gtccgccctg ggtcctggcc
      841 aatccggggg gcggcatacg ctaccggcaa tgccggggga cgtcaaacgg gcggcaacta
      901 ctgctgccaa tgaacgcggg tgaggattcc gctatcctct ggcgcagcga acaaacgacg
      961 aagaagccac tttcggccag gtttttgagc agcatgagga ttatgtgtcc atggcgaaga
     1021 tgaacgcgtt ctacgcgcag tccggcggtg taacggcggt gatcaatgcg accgcatgcg
     1081 gtgtgataga gaccgcgcgc gaaaacaaga gccgcatcgg aaacgtctat gccggacgca
     1141 acgggatcat cggagccctt aaggaagaca tgatcgatac cggcaaagag actgcccgca
     1201 cgatcgccgc gttgcggcac acgccgggag gcgcgttcgg ctcagctcga tacaagctga
     1261 aaggcatcga ggagaaccgg gcgcagtacg agcgcctcgt cgaggtcttc agggctcacg
     1321 acatcgggta ctttttttat aacggcggta acgattccat ggataccgcc cacaaggtgt
     1381 cgctggtcgc gaagcgcctc ggctacccgg tcaggtgcct gggcatcccc aagaccgtcg
     1441 ataacgacct gccggtcacc gacacctgtc ccggcttcgg ttccgtcgcc aagtacgtag
     1501 cggtatccac ccgcgaagcg gcgctggatg tcctgtcgat ggcggaaacc tccaccaagg
     1561 tgttcatcct ggaggtgatg gggcggcatg caggctggat tgccgccgcc ggcggactgg
     1621 cgggcgagaa gccggaggaa gccccgcaca tcatcctttt tcccgaaatt cccttcgaaa
     1681 aagatgtgtt ccttaagcgc gtcagggagt gcgtcgaaag cttcggctac tgtgtcatcg
     1741 tcgtcagcga gggagcgcgc tatgaggatg gccggttcct ggccgaagcc ggcacccgcg
     1801 atgcattcgg ccatgcccag ctcggcggcg tcgccccggt ggttgcaaac atggttcgcg
     1861 agaacctggg ctacaaattc cactacgcgg tggcggacta cctgcagcgc tcggctcgtc
     1921 atatcgcctc ggcagtggac gtagaacagg cgtatgccgt tggcaaggcg gccgtcaaaa
     1981 tggctctcgc gggaaagacg gcggtgatgc ccgtgataaa gagggtctcc gactctcctt
     2041 accgctggag aatcgaggaa gcggcactgt cacgcgtggc gaacaaggaa aaaaaactgc
     2101 cacggcgttt catcagcgcc gacggattcg gcatcacggc tgctgcccgg cgctaccttt
     2161 caccgctgat ccggggggag gattatccgc cgtacatcaa cggcttgccg cgatatgtca
     2221 cactgcgaaa cgagcccgta cccaagcggc tcaacacgga ttttgtggta tgatgcgccg
     2281 cctgatcgcg cggcacactg taaaagacca acgaaatgcg gtgtagaaga aaataatctt
     2341 cgacatgcct caatccgctg caggtcattc gccaatccct tttgtataaa ccccggtcga
     2401 tgcgctccgg ggtgtctggt ccagtcagga gaaaaaactg atgtttaacg agttcgcgtt
     2461 gtggatcgcc atcggcgcgg gtgttttggc agtcctgtac ggaatatttt ccgcgcagtg
     2521 gatcctgaaa cagcctgccg gcaacgagcg catgcaagtc atcgcacagg cgatccagga
     2581 gggcgcaggc gcctacatga accgccaata tctcaccatc ggcatggtgg gcatcgtgtt
     2641 gttcatcgtc ctggtggtag cactcggctg gacgactgga atcggctttg ccatcggcgc
     2701 catcttctcg ggactggccg gctatatcgg catgttcgtc tcggtgcgcg ccaacgtgcg
     2761 aacggcggaa gccgccaggc acggcgtaaa tcctgcgctc aacattgcct ttcgcggagg
     2821 cgccatcacc ggtctgctgg ttgtcggctt gggcctcctc ggcgtcgccg gatattacgc
     2881 ggtgttgggc gggatgggcg tcagcaccga cgacgccatc catgcgctcg tcggcctggc
     2941 atttggcggc tcgctgattt ccatattcgc acggttgggc ggcggcatct tcaccaaggg
     3001 cgccgacgtc ggcgctgacc tcgtaggcaa agtggaagcc gggatccctg aagacgaccc
     3061 gcgcaaccca ggcgtaatcg cggacaacgt aggcgacaac gttggcgact gcgccggaat
     3121 ggccgcggac ctcttcgaaa cctacgccgt cacgatcatc gcgacgatgc ttttgggaag
     3181 cctcatagcc gacagtttcg gcagcgctgc cataacctac ccgctcgtgc tgggcgccgt
     3241 ttcgatcatc gcgtccatca tcggcacgtt ctttgtccgc acctcggatg gcggcaagat
     3301 catgaacgcc ctgtacaagg ggctgatcgt ctccggcgcg cttgccgcgg ttgccttctg
     3361 gttcgtcacc aactccatga tgggcgacac cggcaatgcc gggggaatct tcggctccgc
     3421 cctgatcggc ctcgcgctga cggcggcgct ggtcgttata accgagtact acaccgggac
     3481 cgaatataaa ccggtgaaaa cgattgcaaa tgcgtcgctg accggcgatg caacaaacat
     3541 cattgccggg ctcggcatct ccatgaaagc aaccgctttg ccggtcctcg cggtgtgcct
     3601 gagcatctgg ggcgcgtttg ccctggctcc tgaaggcgac tccctggccg ggctgtacaa
     3661 catcgccata gccgcaacgg cgatgttgtc aatgaccgga gtgatcgtag cgctggacgc
     3721 gttcgggcca attaccgaca acgccggtgg tatcgcggag atgtcggacc tgccatcgga
     3781 tgtccgcaac atcaccgacc agctggatgc cgtcggcaat acgacgaagg ccgtcaccaa
     3841 gggctacgct atcggctccg ccggcctcgc cgcactggta ctcttcgccg actacacgaa
     3901 cgcgctggaa tccgcgaaca tcatcgtcga cttcgacctg tcggatcacc tggtgatcat
     3961 cggcctgttc atcggaggtt tggtgcccta cctgttcggc tcgatggcga tggaagcagt
     4021 cggccgcgcc gccggcgcag tcgtgaacga agtacgccgc cagttccgcg agatcccagg
     4081 gatcatggaa ggaaccggaa agccagacta cggccgtgcc gtcgacctgc tcacgaaagc
     4141 cgcgatcaag gaaatgatca tcccgtcgct gctgccgatc ctcgttccgc tggccgtcgg
     4201 cctgctgctc gggccaaaag cgctgggcgg cgtgctgctc ggcacgatca tcacgggtct
     4261 cttcgtcgcg atatccatga ccgccggcgg cggcgcgtgg gacaacgcga agaagtacat
     4321 cgaggatggt aactgcggcg gcaagggctc cgatgcccac aaggccgccg tcaccggaga
     4381 caccgtcggt gacccctaca aggacaccgc cggccccgcg atcaacccgc tgatcaagat
     4441 catcaacatc gtggcgctgc tctgcgtgcc gctgctcggc tagcggagcc tccgctcatc
     4501 ggttcgcgca atcgggaggc gagctcacgc tcgcctcccc caacgtcgtt
//
LOCUS       DASUOM010000105         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_805425,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000105 DASUOM010000000
VERSION     DASUOM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805425"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFY27_02130"
     CDS             <1..626
                     /locus_tag="VFY27_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5764337.1"
                     /translation="YTLLPEFLIRFLAWILINTLYRIRAIGLENIPREGPAVLVCNHV
                     SFVDALVVGGTIRRPVRFVMYYRIFEIPLLKFLFNTAKAIPIASAKENEALLEEAFDR
                     IDAELETGNVVCIFPEGAITRDGEIHRFRPGIERIIERHPVPVIPMALGGLWGSWFSR
                     RSNGTLRRLPGKLFQLVTLRIGEPVPPGEVSARGLELLVRTLRGDVR"
     gene            complement(711..>2334)
                     /locus_tag="VFY27_02135"
     CDS             complement(711..>2334)
                     /locus_tag="VFY27_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764338.1"
                     /translation="EVLAELSVAGAPSLVAIDAAGDHLAVADYDRAVRIWHMRDRRLL
                     AQIALEADPAEIKLSPGGEALGVVYRGSGVAVWRTDAPSAPIVSERGTGDWRLEFSPS
                     GDKVIAGTNRQGYQVYRSSDGALAGAPLGAGPAQGPKVLAFAGDESFVLTGGPSAQTR
                     LWRAPVSPVISDHAPGDDQPSGHQLWRRSGDAVSALGPGGQRLAIGDYAGHVHILHVN
                     ASAEELAEAGDELNFLGHRSAVAELTFSNDGSLVASAGSAGSIRIWDTATGLPRPYLG
                     GGSARAVDELQFSPSANRLAVLGGRRVLVMEVETGNVLTDVDLGELHSSMTFADDDRL
                     MLGGESGTLRALAIDRTGNWNLRNIWTGSVPLRNLEMSSRKNLLVIVDSTNKAQLLDL
                     ETGRIGASSLELPDAVNDIVFSPNETRVLFRTARWIHRASISPAGLTWLDAVRTPRVM
                     AGSRMVFEPAAVEPKPPEAGSFGDPLGNRVLLLTRDAGFAEVAVIDFSYETGPTLIGN
                     HDKLLVEWREKLGREAPPPMMRSASRDQESAR"
BASE COUNT          445 a          734 c          712 g          443 t
ORIGIN      
        1 tctacacgct gctgccggaa ttcctgatcc gtttccttgc gtggatattg atcaacactc
       61 tctaccggat acgcgctatc ggtttggaga acattccgcg ggaaggtccc gcagtgctcg
      121 tctgcaatca cgtgagtttt gtcgatgctc ttgtcgtggg cggtacgata cgccgcccag
      181 tgcgcttcgt gatgtactac aggatcttcg agatccccct gttgaagttc ctgttcaata
      241 cggccaaggc gataccgatt gcttccgcga aagagaacga agcgctgctg gaagaagcgt
      301 ttgacaggat cgatgccgaa ctcgaaaccg ggaacgtcgt ctgcatcttt ccggaaggcg
      361 ccattacgag ggatggtgag attcatcgct tccgcccggg gatagagcga attatcgaac
      421 gtcaccccgt accggtcata cccatggcgc tgggcgggct ctggggaagc tggttcagcc
      481 gccgcagcaa cgggactttg cgtcgcttgc cgggaaagct gttccagctc gtaactttgc
      541 gcatcgggga acctgttcca cctggtgagg tgtcggctcg gggactggag ctcctcgtgc
      601 gcacgttacg cggcgacgtt cgctgacggc gctgcacgtc ccgcctgggc tacgcaacga
      661 caaaagcggg aaaatggtcc gaagtagaac gttcgcagca gcaatacatt ttaccgcgca
      721 gactcctggt cgcgggaggc gctgcgcatc atcggaggcg gagcttcgcg cccgagcttc
      781 tcgcgccatt ccacaagaag cttatcgtgg ttgccgatta gcgttggacc ggtttcgtaa
      841 ctgaagtcaa ttaccgcaac ttccgcgaag ccggcgtcgc gcgtcaggag aaggaccctg
      901 ttgccgagcg gatcgccgaa tgaacctgcc tctggcggct tcggttcgac ggcggccggt
      961 tcgaagacca tgcgcgagcc agccatgacg cgaggcgtcc gtaccgcatc cagccaggtg
     1021 agaccggcag gcgaaatgct ggcccgatga atccagcgag cagtccggaa cagcacgcga
     1081 gtctcattcg gactgaagac gatatcgttc acggcatcgg gcaactccag gctggaagca
     1141 ccgatcctgc cggtttcgag atccagcaat tgcgccttgt tcgtggaatc cacgatcacc
     1201 agcaaattct tccgggatga catctcgagg ttgcgtagcg gcacggagcc ggtccagatg
     1261 ttgcgcaggt tccagttgcc ggtacggtcg atcgcgagcg cacggagggt cccgctttcc
     1321 cctccgagca tcaggcggtc atcgtcggca aacgtcatac tcgagtgcag ttcgcccagg
     1381 tccacgtcgg tgaggacgtt gcctgtctct acttccatga ccagtacgcg acggccgccc
     1441 agtacggcca gccggttcgc cgatggtgaa aactgaagct catcgaccgc gcgcgccgaa
     1501 ccgccaccga ggtaaggccg cggcaatcct gttgcggtat cccagatgcg aatgctgcct
     1561 gcggagcctg cgcttgcgac caatgaacca tcattgctga acgtcaattc cgcgacggcg
     1621 ctgcgatgcc ccaggaagtt gagctcgtcc cccgcttcgg ccagctcttc cgcactggca
     1681 ttcacatgca gtatgtgaac gtggccggcg tagtcgccga tggccagcct ctgcccgccg
     1741 ggaccgagcg cgctgactgc gtctccggaa cggcgccaca actggtggcc gctcggctga
     1801 tcgtcaccgg gcgcatggtc ggagataacc ggcgacaccg gtgcacgcca cagccgcgtt
     1861 tgtgcactcg gtccgccggt cagtacaaag ctctcgtctc ccgcgaatgc gagcacttta
     1921 ggtccctgcg caggcccggc gccgaggggc gcaccagcca acgcaccgtc gctgctgcga
     1981 tagacctggt agccttggcg attcgtgccg gcgattacct tgtcaccgga aggcgaaaat
     2041 tcgagcctcc agtcgccggt tcccctttcg gatacgatcg gggcagatgg cgcatccgtt
     2101 ctccacactg cgacgcccga cccgcggtaa acaacaccca gcgcctcgcc gcccggtgaa
     2161 agcttgattt cggcaggatc cgcctcgagc gcaatctgtg cgagcaggcg acggtcgcgc
     2221 atatgccaga tacggacagc gcgatcgtag tcggcgaccg cgagatgatc gcccgccgca
     2281 tcgatcgcaa ccagcgacgg cgctccggcg acgctaagtt cggcaaggac ctca
//
LOCUS       DASUOM010000106         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_189141,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000106 DASUOM010000000
VERSION     DASUOM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189141"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFY27_02140"
     CDS             <1..647
                     /locus_tag="VFY27_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5764339.1"
                     /translation="LVLTRAVTNLEMRRLFGVGVPRHAIDIQKSIRINAPVQKVFELW
                     DNFENFPLFMTHVRHVRRIRTGEDKERWRWGVTGPTGTELEFDSTVTAREENRLIAWR
                     TVGEAFVQHAGQVRFLDNADGSTTVDIKMVYNPVAGAVGHAIARMFGTDPKRQMDDDL
                     LRVKSFLETGKLPRDAAEHGAEVPPALKEIAREHGAGGNGAGQQGAERERVRPG"
     gene            583..1365
                     /locus_tag="VFY27_02145"
     CDS             583..1365
                     /locus_tag="VFY27_02145"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF006672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEX5764340.1"
                     /translation="MAQEETARGSRAQNGSAYVRGDRVGQLHLPAAILTGYAAITSDE
                     SKQLAGVSVEILEQPYLAEAQALCLVARLFGERQWCLATGGNFSVRIDRTRCLITQSG
                     TDKAQLSVDDLMVCDLNGAPADTSLRPSAETALHTCLYKLDERIASVLHTHSVLSTVT
                     SRAEYSDLVIQGFEMQKAIDGVKSHDERLILPILENTQDVTQLAKTVRERYALGHMRA
                     CGFLVRGHGLYAWGETLAAAKRHVEGLEFLLACKWQERLLNP"
     gene            1362..2042
                     /gene="mtnC"
                     /locus_tag="VFY27_02150"
     CDS             1362..2042
                     /gene="mtnC"
                     /locus_tag="VFY27_02150"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEX5764341.1"
                     /translation="MIRIRAIVTDIEGTTSSISFVHEVLFPYAARQLPNYVRAHANDS
                     EVAALLQDLREEAGEPDADVERLVAILLQWIAEDRKATPLKTLQGLVWEQGYRSGDFT
                     GHVYADTAPAMQRWSDRGARIFIYSSGSVKAQQLLFGHSDAGDLTPLIDGYFDTHVGN
                     KRETASYRAIAEEVGLPAEQILFLSDTPEELDAAADAGMKTMQLARDEVMQSSRHPVA
                     RNFDEVVI"
     gene            2091..>2475
                     /locus_tag="VFY27_02155"
     CDS             2091..>2475
                     /locus_tag="VFY27_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX5764342.1"
                     /translation="MSRLSVYAESQPDRPLVETSDFDRIASELGAAGIRFERWEAGQD
                     LPDDADSDTIIAAYRAEIDRLIEERGYQTFDVVSMTPDHPQKSAFRSKFLEEHTHSED
                     EVRFFVRGQGLFVIHVDGKVYSMLCE"
BASE COUNT          479 a          784 c          788 g          424 t
ORIGIN      
        1 tgctcgtcct gacgcgcgcc gtaacgaacc tcgagatgcg ccgcctgttc ggcgtcggtg
       61 tgccgcgtca cgcgatcgac atacagaaga gcatccggat caacgcgccg gtgcagaagg
      121 tcttcgaact ctgggacaac ttcgagaact tcccgctgtt catgactcac gtgcggcacg
      181 tgcgccgcat caggaccggc gaggacaagg agcggtggcg ctggggcgtc acgggaccga
      241 ccggcaccga gctcgagttc gattccaccg tcaccgcccg cgaagaaaat cgcctgatcg
      301 cctggcgcac cgtgggcgag gcgttcgtgc agcacgcggg gcaggtacgt ttcctggaca
      361 atgccgacgg gtcgaccacc gtcgatatca agatggtcta taaccccgtc gcgggtgcgg
      421 tggggcacgc cattgcgcga atgttcggaa ccgacccgaa gcgacagatg gacgacgacc
      481 tgttgcgggt gaagagcttc ctcgagaccg gcaagctgcc gcgggatgcg gccgaacacg
      541 gcgcggaagt gccgcccgcg ctcaaggaga tcgcacgcga gcatggcgca ggaggaaacg
      601 gcgcggggca gcagggcgca gaacgggagc gcgtacgtcc ggggtgacag ggttggccaa
      661 ctgcacctcc ctgcggctat attgacgggc tatgcggcaa tcacttctga cgaaagcaaa
      721 cagcttgccg gcgtgagcgt cgagatactc gagcagccct accttgccga ggcgcaggcg
      781 ctttgcctcg tcgcgcgcct tttcggcgag cggcagtggt gcctggcgac gggcggcaac
      841 ttctccgtgc gcatcgacag gacccgttgc ctcataaccc aatccggtac cgacaaagcg
      901 caactatccg ttgacgacct gatggtctgc gacctcaatg gagcgccggc cgatacctcg
      961 ctcaggccct ccgcggagac ggctctgcat acttgcctgt acaagctcga cgagcgcata
     1021 gcttccgtgc tgcacacgca ctcggtcctc tccacggtga cctcccgcgc ggagtattcc
     1081 gatctcgtca tccagggttt tgagatgcag aaggcaatcg atggcgtgaa gtcgcacgac
     1141 gagcggctca tcctgccgat actcgagaac acgcaggacg tcacgcagct tgcgaagacc
     1201 gtgcgcgagc gatatgcgtt ggggcacatg cgtgcgtgtg gtttcctggt gcgcggacac
     1261 ggcctttatg cgtgggggga gaccctcgcc gccgcgaaac ggcacgtcga aggactggag
     1321 ttcctgcttg cctgcaagtg gcaggagcgg ttgcttaacc catgattcgc ataagggcga
     1381 tcgtcaccga catcgaaggc acgaccagct cgatcagctt cgtccacgag gtgctgtttc
     1441 cctacgccgc gaggcagctc ccgaattacg tgcgggcgca cgcgaacgat tccgaggtcg
     1501 ccgctttgct gcaggacctg cgcgaggaag ccggcgaacc cgatgcggat gttgagcggc
     1561 ttgtcgctat cctgctgcaa tggattgcag aagatcgcaa ggccacgccg ctcaaaaccc
     1621 tgcaaggcct ggtgtgggag cagggctacc gcagcggcga tttcaccggc cacgtctacg
     1681 cagacaccgc accggcaatg cagcgctggt ccgaccgcgg cgcgcgtatc ttcatctact
     1741 cgagcggttc ggtgaaggcg cagcaactgc tgttcggcca cagcgatgcc ggcgatctca
     1801 cgcctctcat cgatggctac ttcgatacgc acgtcggaaa caagcgcgag accgcctcct
     1861 atcgcgcaat agcagaagag gtcggcctgc ccgcggagca gatcctgttc ctctccgaca
     1921 cgccggaaga actcgacgcc gccgccgacg ccggcatgaa aaccatgcag ctggcccgcg
     1981 acgaagtcat gcagtcgagc cggcacccgg tcgcgcgcaa tttcgacgaa gtggtgattt
     2041 aggacgtggt gatctcgtac agtgttttcc cagcctgcta gaattccgcc atgagccgcc
     2101 tgagcgttta cgcagaatca cagcccgacc ggccgctggt cgagacctct gactttgacc
     2161 gcatcgcctc cgagctgggc gccgccggca tccgcttcga gcgctgggag gctggccagg
     2221 acctgccgga cgatgcggat tccgacacca tcatcgccgc ctaccgcgcg gagatcgacc
     2281 ggctgatcga ggagcgcggg taccagacct tcgacgtcgt cagcatgacg cccgaccatc
     2341 ctcaaaagag cgcgtttcgc agcaaattcc tggaggagca cacgcacagc gaggacgagg
     2401 tgcgtttttt cgtgcgcgga cagggacttt tcgtcattca cgtcgacggc aaggtctatt
     2461 cgatgctgtg cgagc
//
LOCUS       DASUOM010000107         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_149265,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000107 DASUOM010000000
VERSION     DASUOM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149265"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFY27_02160"
     CDS             <1..1446
                     /locus_tag="VFY27_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764343.1"
                     /translation="EYSMYGAVPLSTVKKALLDLKDEGRLDRVKKVDLTNCTFDGHIY
                     NTRRVMEECLAIKPDLVFLWDEAWFGFACFSPFLRSRTAMGAAAEIGSWMRDPKSIET
                     FERQQAELGKSPSKETLLATRLVPDPRKIRLRVYQTNSTHKSMSALRQGSMVLVKDVD
                     YYTVESQFREAVFTHASTSPNQQLIASLDIARRQMELEGYGLVMNAIEIALAIRREVN
                     SHPLISKYFRVLGADAMIPAEFRSSGFVDFLQPEVRWVDQVRSMREDEFCLDPTRMTL
                     VCGTAGFDGTEFKALLANTYDIQLNKTSRNSVLLQSNINNTRSDIAHLIRVLLEICHG
                     IEDRLAQGGEAGHTAFEARVNSLMRDVPDLPDFSRFHEIFRSDAGKTTPEGDMRSAFY
                     GGYDPAACEYVPINSEECDKRLRDGPEMVSANFVIPYPPGFPIMVPGQVVTEGAIDFM
                     RKLDVKEIHGYERTKGLKLIKPDVVGAKLRR"
     gene            1499..>2019
                     /locus_tag="VFY27_02165"
     CDS             1499..>2019
                     /locus_tag="VFY27_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764344.1"
                     /translation="MFEWFANTLRTYPEIAIFLSLAFGYYFGSFTYKGLGLGAVTATL
                     IAAVIIGQLGITVTGPLKPFFFLMFLFAIGYAVGPQFVRGIAKDGLPQALFAAVVCVF
                     CLAGAYIAVIAAGYDVGSAAGLYAGSQTISASMGLATDAINRLGLPPEETKRLLDGMP
                     VAYAVTYIFGTMGS"
BASE COUNT          402 a          619 c          589 g          409 t
ORIGIN      
        1 gagtactcga tgtacggcgc ggtcccgctg tcgaccgtta agaaagcgct gctcgacctg
       61 aaggatgaag ggcgtctcga tcgcgtgaaa aaggtcgacc tgaccaactg caccttcgac
      121 ggccacatct acaacacccg acgcgtgatg gaggaatgtc tggcgatcaa gcccgacctg
      181 gtcttcctct gggacgaggc ctggttcggg ttcgcctgct tctcgccatt cctccggtca
      241 cggaccgcca tgggcgccgc agccgagatc ggtagctgga tgcgcgatcc gaaatcgatc
      301 gagactttcg aacgccagca ggcggagctt gggaagtccc cgtcaaagga aactctgctc
      361 gcgacccgcc tggttccgga cccccggaaa atccgcctgc gcgtatacca gaccaactcg
      421 acgcacaaat cgatgtcggc gctgcgtcag ggatccatgg tcctggtcaa ggacgtcgac
      481 tactacacag tggaatcaca gttccgcgaa gccgtcttca cgcacgcctc caccagcccg
      541 aaccagcagc tcatcgcgag cctggacatc gcacggcgac agatggagtt ggaaggctac
      601 ggtctggtca tgaatgcgat cgagatcgcg ctcgccattc gccgggaagt caattcgcac
      661 ccgctgatct cgaagtactt cagggtgctc ggtgcggacg caatgatacc ggccgagttc
      721 cgcagcagcg ggttcgtcga cttccttcag cccgaggttc ggtgggtgga tcaggtgcgc
      781 agcatgcgcg aggacgaatt ctgcctcgat cccacacgta tgacgctcgt ttgcggaacg
      841 gccggtttcg atggcaccga gttcaaggcc ctgcttgcca acacctatga catccagctc
      901 aacaagacgt cacgcaattc ggtcctcctg cagtccaata tcaacaacac ccgtagcgac
      961 atcgcccacc tgatccgcgt ccttctcgag atttgccacg gcatcgagga ccggcttgcc
     1021 cagggcgggg aggccggcca caccgcgttc gaagcgcgtg tcaattcact gatgcgggac
     1081 gttccggatc tgccggactt cagccggttc cacgagatct tccgaagcga cgccggaaaa
     1141 acgacacccg aaggcgacat gcgctcggcc ttctatggag ggtacgaccc ggccgcttgc
     1201 gagtatgtac cgataaactc cgaggagtgt gacaagcgat tgcgggacgg gccggaaatg
     1261 gtttccgcca acttcgtcat cccttatccg cccggtttcc ccatcatggt tcccgggcag
     1321 gtcgtcaccg agggggctat cgatttcatg cgcaagctcg atgtcaagga gatccatggc
     1381 tatgagcgga cgaaggggct gaagctgata aagccggacg tcgtcggggc aaagttgcgc
     1441 cgatgagact gagcgccggc aagtagagaa cacgtcgtcg cagtaaggag cggggatcat
     1501 gtttgaatgg tttgcgaaca cgcttcgcac atatccggaa attgcaatct ttctctcttt
     1561 ggctttcgga tactatttcg gctctttcac ctataagggt ctcggccttg gcgcggtgac
     1621 ggcgacgctc attgccgccg tgatcatcgg ccaactcgga attacggtca caggtcctct
     1681 taagccgttc ttcttcctga tgttcctgtt cgctatcggc tatgcggtcg gtccccaatt
     1741 cgtccgcggc atagcgaagg acggacttcc gcaggcgctc tttgcggccg ttgtctgcgt
     1801 gttctgccta gccggtgcct acattgccgt tatagcggca ggctacgacg tcggctccgc
     1861 tgcagggctt tatgccggct cccagacgat ctccgcctcg atgggtcttg cgacggacgc
     1921 catcaaccgc ctcggcctgc ctcccgagga aacaaaaaga ctactcgatg gaatgccggt
     1981 cgcgtacgcc gtgacctaca tcttcggaac gatggggtc
//
LOCUS       DASUOM010000108         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1118153,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000108 DASUOM010000000
VERSION     DASUOM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118153"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFY27_02170"
     CDS             <1..1169
                     /locus_tag="VFY27_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX5764345.1"
                     /translation="MPDFGSETVECTAAAGRVLRQAVVAERDQPPFDRVTMDGIAIRF
                     DAFCRGVASFRIAGTQHAGDPVLALPDEMSCIEVMTGAVLPEGSDCVIPVERIGLTNG
                     SAEVEGGYSAERQQFVHPRGSDHRQGTEVISSGALLTPAEIAIVASSGLQFVEVGRRP
                     VVRVISTGNELVPPGQPVLPHQVRSSNGPALVAMLRGQGFSDCRHEHLRDDPEALHIE
                     IGRHLEEAQVLILSGGVSMGQADFVPQVLADLGVKPVFHKISQRPGKPMWFGIGKEGQ
                     AVFALPGNPVSTLVCCRHYVLPALLDACGRGGRSLEWAVLTEDVTFKADLTYFIPVTL
                     HSDVDGTMRARPVLTNTSGDFTALGGTDGYVELAREQSYFPQGSPVPLHRWNSP"
     gene            1252..1650
                     /locus_tag="VFY27_02175"
     CDS             1252..1650
                     /locus_tag="VFY27_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5764346.1"
                     /translation="MPFMKPYQEHTYALLRIVAGYMLLWHGAQKLFGVLGADQASGYM
                     FWLAGPIELIGGVLVMIGLFTRPAAFICSGFSAAAYWMAHGSFDALPGSALLPITNRG
                     ELAVLYCFTFLYIAARGAGIWSVDGAMKRS"
     gene            complement(1740..>2148)
                     /gene="rho"
                     /locus_tag="VFY27_02180"
     CDS             complement(1740..>2148)
                     /gene="rho"
                     /locus_tag="VFY27_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746305.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5764347.1"
                     /translation="VLTGGVDANALHRPKRFFGAARNIEEGGSLTILATALVDTGSKM
                     DEVIYEEFKGTGNMEIHLDRRISEKRVFPAININRSGTRKEEYLTEPDELQKMWVLRK
                     VLHPMDELAAVEFLLNKLQDTKTNTDFFEAMKR"
BASE COUNT          382 a          639 c          686 g          441 t
ORIGIN      
        1 cgatgccgga cttcggcagc gaaaccgtcg aatgcacggc agcggccgga cgcgtcctgc
       61 gccaggccgt cgttgcggag cgcgaccagc cgccgttcga ccgcgtgacg atggacggca
      121 tcgcgatccg cttcgatgcg ttttgccggg gagtcgcatc atttcgcatc gccggaacgc
      181 agcatgccgg cgacccggta ctcgcactgc ccgacgagat gtcctgtatc gaagtgatga
      241 cgggcgccgt gttgcctgag ggcagcgatt gcgttattcc ggtcgaacga atcggtctca
      301 ccaatggcag tgccgaggta gaggggggat attccgcaga acggcagcag ttcgttcatc
      361 cccggggttc ggatcaccgc cagggcacgg aagtgatttc atccggcgcc ttgctaactc
      421 ctgcggaaat cgcgatcgtc gcatcctctg gacttcagtt cgtcgaagtg ggccggcgtc
      481 cggtagtccg ggtgatatcc accggcaacg agctggtgcc gccgggacag ccggtgttgc
      541 cgcaccaggt tcgttcatcg aacggtccgg cgctggtggc gatgctgaga ggccagggtt
      601 tcagcgattg ccggcatgaa catcttcgcg atgacccgga ggcgctgcac atcgaaatcg
      661 gccggcacct cgaggaagcg caggtgctga tcctgagcgg cggcgtctcc atggggcagg
      721 cggacttcgt gccgcaggtg ctcgcggatc ttggcgtgaa accggtcttt cacaagataa
      781 gccagcggcc ggggaagccg atgtggttcg gtatcggcaa ggagggacag gcggtgttcg
      841 cgttacccgg aaaccctgtg tcgaccctcg tctgttgccg ccactacgtg ctccccgcat
      901 tgctcgatgc ctgcggacgc ggcgggcgca gcctggaatg ggcggtcctg accgaggacg
      961 ttacgttcaa agccgatctg acgtatttca tcccggtcac gctgcatagc gatgtcgacg
     1021 gtaccatgag ggcaagaccg gtgctcacca atacctcagg cgactttacg gcactcgggg
     1081 gaaccgacgg ctacgtggaa ctggcgcgtg aacaaagtta tttcccgcaa ggcagccctg
     1141 ttccgctgca tcgatggaat tcgccttaag ccttttgaaa gctggttggg gcactttgcc
     1201 cgtgttacaa agtgggctca agtctccatc accagccagg agggtcatga catgccattc
     1261 atgaagcctt accaggaaca cacctacgcg ctgttgcgca ttgtcgcagg ctacatgctc
     1321 ttgtggcacg gcgcacagaa gctgttcggg gttcttggtg cagaccaggc gagcggttac
     1381 atgttctggc tggccggccc catcgaactg ataggaggag tgctggtgat gataggactg
     1441 ttcacccgtc cggccgcctt tatctgcagc ggcttcagcg ccgccgccta ctggatggcc
     1501 cacggctcgt ttgatgcgtt gccgggctcg gccctgttgc ctatcacgaa caggggcgaa
     1561 ctggcggtgc tctattgctt cactttcctc tatatcgcgg cgcgcggcgc cggaatctgg
     1621 agcgtcgacg gggccatgaa gcgcagctag gcgttgattg cgcgcctgta ggaggccgac
     1681 tcacggcgac cggcgacgat gcaagcaaga agatgtcgcc cgcgaggcgg gcgagcagct
     1741 tagcgtttca tcgcctcgaa gaaatcagtg ttggtcttgg tgtcctgcag cttgttgagc
     1801 aggaattcca ccgcggcgag ttcgtccatc ggatggagta ccttgcgcaa gacccacatc
     1861 ttctgcagct cgtccggctc ggttaggtat tcctctttgc gcgtgcctga acggttgatg
     1921 ttgatggccg ggaaaacacg cttctcggaa atgcgccgat ccaggtggat ctccatgttg
     1981 ccggtgccct tgaactcctc gtagatgacc tcgtccatct tcgagccggt atcgaccagc
     2041 gccgtcgcaa gtatcgtaag gctgccgcct tcctcgatgt tccgcgccgc gccgaaaaat
     2101 cgcttcggcc ggtgcagcgc gttcgcatcg acaccaccgg tcagtacc
//
LOCUS       DASUOM010000109         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1360072,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000109 DASUOM010000000
VERSION     DASUOM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360072"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VFY27_02185"
     CDS             <1..1347
                     /locus_tag="VFY27_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764348.1"
                     /translation="MGPLNDEMKREMVARQGDIKYDIEWTTLNEYLEYLAEKGISPNV
                     ASYVGATTLRVHEVGYENRPANEAELGRMQELVREAMREGALGVGSSLIYAPANFADT
                     DELIALVSAAAEFGGAYISHIRSEGDRLEEAVTELLKIASMTGAPAEIYHLKASGKPN
                     WYKLQNVFAMIEAAREAGLRITANMYTYPASATGLNASMPLWVQEGGHDAWIARLKDP
                     QVRARVVAEMKDPDADWENRFVQAGPENILLLSFVNDDLKPLMGKTLKEVADERGTDP
                     AETAIDLVIEDNSRVGVSYFMMSEDNVRRKVAQPWMSFGSDAGAPAAEGVFLKSNTHP
                     RAYGTFARVLGKYSREEGVIPLHEAVRRMTSLPATNIGIRGRGLLAEGQFADVVVFDP
                     ETITDHATFEEPHQYATGVDHVFVNGVQVLSDGEHTGATPGRVVRGPGWRGWPQNQ"
     gene            complement(1429..>2178)
                     /locus_tag="VFY27_02190"
     CDS             complement(1429..>2178)
                     /locus_tag="VFY27_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764349.1"
                     /translation="LTLVGWLHIVLLLATFVGLLTDERVITGVNAWLKPAKFMFSIAV
                     YLWTIAWFSKYIRRPRWLLASVSIVIATTMIVESTCILLQAARGTTSHYNVATDFDAA
                     IFQTMGIMIGIDMFMALVILFMFSRPSIRLRPAYLWSIRAGLVIFLAGGAIGGLMVAH
                     GAHTFGAPDGGPGLPLLNWSTVGGDLRIAHGLALHALQILPLTGYGISCWSAVPRTAG
                     ELALLAIAFVAYGAAVFMLYRQAIAGTPLLG"
BASE COUNT          482 a          657 c          671 g          368 t
ORIGIN      
        1 atggggccgc tcaatgacga gatgaagagg gagatggtgg cgcgccaggg cgacatcaaa
       61 tacgacatcg aatggacaac gttgaacgaa tacctggagt acctggccga aaaaggcatc
      121 tctccgaacg ttgcctccta cgtaggagcg acaaccctac gggtgcatga agtcgggtat
      181 gaaaaccgtc ccgcaaacga ggccgaactc ggccgtatgc aggagttggt gcgggaagcc
      241 atgcgcgaag gtgccctggg cgtcggctct tcgctcatat acgcgccggc aaatttcgct
      301 gataccgacg agctgatagc gctcgtgagt gccgccgccg aattcggcgg cgcctacatc
      361 tcgcatatcc gttccgaggg cgatcgcctc gaggaggctg tcaccgaact tctgaagatc
      421 gcgagcatga cgggcgcacc ggcggaaatt tatcacctga aggcatcagg gaagcccaac
      481 tggtacaagc tgcaaaacgt attcgcaatg atcgaggccg ccagggaagc cggcttgcgc
      541 atcacggcga acatgtacac gtacccggca agcgctacgg gacttaacgc ctcgatgccg
      601 ctgtgggtcc aggagggcgg acacgatgcc tggatagccc ggctgaagga tccgcaggtg
      661 cgcgctcgtg tggttgccga gatgaaggac ccggacgccg actgggagaa tcgattcgtg
      721 caggctggac cggaaaacat cctgctgctc agttttgtca acgacgacct gaagccgctc
      781 atgggcaaga ccctgaagga ggtggccgac gagcgcggca cggatcctgc ggagaccgca
      841 atcgatctcg tgatcgagga caacagccgc gtcggtgtca gctacttcat gatgtccgag
      901 gacaacgtcc gccgcaaagt ggcacagccg tggatgagct tcggttccga cgccggcgcg
      961 cccgccgccg aaggtgtttt cctgaagtcg aacacccacc cgcgcgcata tggcacgttt
     1021 gcccgggttc tcggcaagta ctcgcgcgag gaaggcgtca tcccgctgca cgaggcggtc
     1081 cgccgcatga cctccctccc cgccacgaac atcgggatac ggggccgcgg cttgctcgcg
     1141 gaaggtcagt ttgccgacgt ggtcgtgttc gatcccgaga cgatcaccga ccacgcaacc
     1201 ttcgaggaac cccaccagta cgctaccggt gtcgatcacg tcttcgtcaa cggtgtccag
     1261 gtactcagtg acggcgagca caccggcgcc actccgggcc gggtcgtccg cggtccgggt
     1321 tggcgcggct ggccccagaa tcagtaaagg ccaggggacg aaatttattt ttttctgaag
     1381 agcgacctgt cctctctcag ccacagaaaa aaataaacct gtcccctttt agccgagcag
     1441 cggtgttccc gcgatcgcct ggcgataaag catgaacacc gcagcgccgt aggcgacgaa
     1501 cgcgatggcg agcaacgcga gttcgcccgc ggttcgtggg accgctgacc agcaactgat
     1561 tccatagccg gtgagcggca gtatctgtaa ggcgtgaagc gcgagaccgt gcgcgatgcg
     1621 caggtctccg cctacagtgc tccagttcag gagcggcaat cccggacctc cgtcaggcgc
     1681 gccgaatgta tgcgctccgt gggccaccat cagtccgccg atggcgccgc ctgccaggaa
     1741 tatcacgagg ccggcacgaa tcgaccacag gtaagccggt cgaaggcgga tcgaaggcct
     1801 gctgaacatg aacaggatga ccagcgccat gaacatgtcg atgccgatca tgatgcccat
     1861 tgtctggaag atcgccgcgt cgaagtccgt agcgacgttg tagtgcgacg tagttccccg
     1921 cgcggcctgc agcagaatgc aggttgactc gacgatcatc gttgtcgcaa tcactatgct
     1981 gacggaagcc agcagccagc gcggacgcct gatgtatttg ctgaaccacg ctatcgtcca
     2041 gagatagaca gcgatggaaa acatgaactt tgcaggctta agccaggcgt tcacgcccgt
     2101 gatgacccgc tcgtccgtaa gcagcccgac aaacgtggca agcaacagca caatatgcag
     2161 ccagcccacc agcgtaag
//
LOCUS       DASUOM010000110         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_876425,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000110 DASUOM010000000
VERSION     DASUOM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876425"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFY27_02195"
     CDS             complement(<1..189)
                     /locus_tag="VFY27_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764350.1"
                     /translation="MKPDPTTPGNASEAGKARAATPTTDTVADAAHGAVERAADSVAK
                     AEQRIREKAALSEQQLREK"
     gene            complement(387..965)
                     /locus_tag="VFY27_02200"
     CDS             complement(387..965)
                     /locus_tag="VFY27_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5764351.1"
                     /translation="MDNKTYLAILASGSLFAIGLAGCDAGDPGDPATPEPESSAMYED
                     TEDATETMADRTSEAGDELQQSGQQAEQELDQRTQQAQDRMANAGDSAMDSLDTLGTD
                     EIVGKSVVSQQGEEIGTIDEVVMDTNSQQQLAVIDVGGFLGIGEKSVAISFDQLELSD
                     DGRVQSDLTRETLQSQPEYDPAQYGEAAEQME"
     gene            complement(1212..2012)
                     /locus_tag="VFY27_02205"
     CDS             complement(1212..2012)
                     /locus_tag="VFY27_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5764352.1"
                     /translation="MVSLPYSVAFLLLFVGACATSPLGRDQLILFPDEQVAQMGVTAF
                     KELKQNTPVSRDTDVNAYVSCVADAITTVLDQGQASAPQQWEVSVFAEEQVNAFALPG
                     GKIGVYEGLLSVAETQDQLATVMAHEVAHVLARHSNERISTQYATSTGLEVASVLAGQ
                     SSPAKQTLFGLLGLGAQVGVLLPFSRKQETEADLLGLELMAKAGFDPEQSVQLWQNMA
                     AASGSAPPEFLSTHPSGESRIRALQQYMPKVLPLAQQARSSGVRPDCR"
     gene            complement(2100..2300)
                     /locus_tag="VFY27_02210"
     CDS             complement(2100..2300)
                     /locus_tag="VFY27_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX5764353.1"
                     /translation="MKDSMFGGAAAFFGIALVAAWMGYSGFAAMFMNIAWILFLFGLV
                     FAALFSIYGQATRQESGEGSRR"
     gene            complement(2359..2580)
                     /locus_tag="VFY27_02215"
     CDS             complement(2359..2580)
                     /locus_tag="VFY27_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764354.1"
                     /translation="MVKRVNKVRKSAILEHLLDLERQIRSELARASMERKSAGMTEAR
                     APRSRTSEESISASREDRIMPEIAQRYLH"
     gene            complement(2749..3018)
                     /locus_tag="VFY27_02220"
     CDS             complement(2749..3018)
                     /locus_tag="VFY27_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764355.1"
                     /translation="MPPTPGSGRNFRRPGPAETVPESGILEDCALRLYTEGPGDFTLP
                     LSYFDYDAIPDLNDVIATLAGGKREYTGAELTFRKRRTPEDRFSG"
BASE COUNT          629 a         1110 c         1063 g          754 t
ORIGIN      
        1 cttctctcgc agttgctgct cactcaaggc tgctttttcc ctgatacgtt gttccgcctt
       61 cgcaacgctg tccgcggcgc gttcgacggc gccatgcgcg gcgtctgcga cggtgtcagt
      121 ggtcggcgtg gcggctctcg ccttaccggc ttccgacgcg ttgccggggg tggtgggatc
      181 tggtttcatg tggttattgc tcctggttgc tgctctatat tgctctttgt tgcggccgct
      241 tgcgaccgtt cttccttaag gtcccgatcg ggccggatcc gtttgtgacg gaggtatcga
      301 aaggatgtga cggaggtatc gggaggagct gcggcagcgc cgcggcgacg agcgacgcag
      361 ccagaggact gcgtcgcttc tctctcttat tccatctgtt ccgctgcttc gccgtactgc
      421 gcggggtcgt attccggctg cgactgcaga gtctcccgtg tgaggtcact ctgtacgcgg
      481 ccgtcatcag acagctcaag ctggtcgaac gaaatggcaa cgctcttctc gccaatgccc
      541 aggaagccgc cgacatcgat caccgccagc tgctgctggc tgttcgtgtc cattaccacc
      601 tcgtcgatgg tgccgatctc ctctccctgc tggcttacga ccgacttgcc cacgatctcg
      661 tccgtgccca gtgtgtcgag gctgtccatc gcgctgtcgc cggcattcgc catccggtcc
      721 tgcgcctgct gggtccgctg atcgagctcc tgctccgcct gctgcccgct ctgctgcagt
      781 tcgtcgcctg cttcggatgt cctgtcagcc atcgtctccg tcgcgtcctc ggtatcttca
      841 tacatcgccg agctttcggg ctccggtgtc gccgggtcac cgggatcacc tgcatcgcac
      901 ccggcaaggc cgatcgcgaa aagcgagccg ctcgccagga tcgcaagata ggttttgttg
      961 tccatgttct aactcctgtt gctgttacgt ttgtctgcga aattccgcct gccgtagagt
     1021 tacaagcaat taccgtgcca tggaaaaggc gctgacgggc ctgctccccc ggctacggcc
     1081 ggccctatcg agggaaccgc atttgatgct aaaaagtacc tcgccaccgt gtaaatcttg
     1141 caaggcgtgt aaatccgctg ccgggcacaa gtcatgttca agacaactct tccgacttgt
     1201 gcggaggctc cctagcggca gtctggcctc acgcccgacg agcgcgcctg ttgcgccagt
     1261 ggcagcacct tcggcatgta ctgctgcaag gctcgaatgc ggctctcgcc cgacgggtgc
     1321 gtcgaaagaa actccggcgg cgcactgcca ctcgcagcgg ccatgttctg ccatagctgc
     1381 acactttgct ccggatcgaa tccggccttt gccatcagct cgagtcccag gagatcggcc
     1441 tccgtttcct gtttcctgct gaacggcagt agcaccccca cctgagcgcc gagacccagc
     1501 aagccgaaca gggtctgttt cgcggggctg ctttgcccgg caaggacgct cgcgacctcg
     1561 agcccggtgc tggtcgcgta ctgggttgaa atgcgctcgt tgctgtgacg tgctaacacg
     1621 tgcgctacct catgtgccat caccgtcgcg agctggtcct gggtttccgc gaccgaaagc
     1681 aagccttcgt acactccgat cttcccgcca ggcaaggcga acgcattgac ctgctcttcc
     1741 gcgaacacgc tgacctccca ctgttgcggc gcggaagcct ggccttgatc gagtacggtg
     1801 gtgatagcgt cagccacaca ggaaacgtag gcgttgacat cggtatcgcg cgagacggga
     1861 gtgttctgct tcagctcctt gaaggcggtg acgcccatct gcgcgacctg ctcgtccggg
     1921 aacagaatca actggtcgcg gcccagcgga gaagtagcgc atgcgccgac gaaaagcagg
     1981 aggaaagcga cgctgtaagg caggctaacc acaacgtacc gcggcagccg gacgcttcgc
     2041 cgggtgcgtc taaacaggga gtttctgtgc tgcggctgcc tcacgcgccc tgctcgccgt
     2101 cagcggcgcg atccctctcc ggactcctgg cgtgtcgcct gtccgtagat cgagaagagc
     2161 gccgcgaaga caagaccaaa aagaaaaagg atccacgcga tgttcatgaa catggctgca
     2221 aatccggaat accccatcca ggccgccacg agcgcgattc cgaaaaatgc cgctgcacca
     2281 ccgaacatag aatccttcac ctgttacttt gttgtttatg cgggcggtct ggtaacgcca
     2341 cccgggaatg cctccttctc aatgcagata tcgttgcgcg atctcgggca tgattcggtc
     2401 ttcgcgactt gcggagattg actcttcgct cgtccgcgac cgcggcgcgc gtgcttccgt
     2461 cataccggcg gattttcgct ccatgctcgc cctggccagt tccgaccgga tctgtctctc
     2521 gagatccagc aggtgctcga ggattgctga cttcctgacc ttgttgaccc gcttaaccat
     2581 cgtgcgctcc tgtaggaatt gagtaagtta acaggagcaa taagcatgcc actccgggtc
     2641 cagctactcc tttatttaca agctaacgaa gccggcactg cattgaaaaa aatgcaccgg
     2701 acggtaatcc ttgcaagtcg tcgcttttgt gcgacagccg gcggcaagtc atcctgagaa
     2761 acggtcttcc ggtgtgcgac gcttgcggaa cgtcaactcg gcgccggtgt attcgcgctt
     2821 gccgccagcg agcgtggcaa tgacgtcgtt caagtccgga atcgcatcgt agtcgaagta
     2881 cgacagcggc agcgtgaagt caccgggccc ctcggtgtac aacctaaggg cgcaatcctc
     2941 gagaatgccc gattcgggaa cagtttcagc cggtcctggc ctgcggaagt tccgcccgct
     3001 gccgggcgtc ggcggcatgg ttatccagga ctttctcgat ccgccagagc tcggtcgcga
     3061 ggagcgaggg cgggggaggt tcgatccgga atacgcgatc cgctcgaggg gaccctgctt
     3121 gcggggccga ccggagcgta tcaccctggc cttgccgtcc gttcgcgtcc agtggcgaag
     3181 taaccggatt tggccggtag agaccgggat gtatatcgtc cgcgccggcg ggcaagacag
     3241 gaggcctgct ccctgactgt tgctgcccgg gatgggggga tttgcctggc atatcgttcc
     3301 ttgagaaggg ggttcagcag ggtgccgaaa cgccttccgg ggaacgagca acttgtgtgc
     3361 caacaatcga gaccgtgcgg aaggcggtca tgggcatgga caggtaaacc ggatagcagg
     3421 cagcatcacg ccgaagccgc ctcttcctca cctttcacca gtgcgacagc cgcgcggatc
     3481 gcatcaacac gattatcgta acgttcagcg ggaggaattc gttcgaggac gccaaggccg
     3541 tcgagcgctc gcaggg
//
LOCUS       DASUOM010000111         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_995962,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000111 DASUOM010000000
VERSION     DASUOM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995962"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3075)
                     /locus_tag="VFY27_02225"
     CDS             complement(<1..3075)
                     /locus_tag="VFY27_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5764356.1"
                     /translation="MGADGNLLAADLNARADALDVARSFIVQAPAGSGKTELLIQRYL
                     KLLAEVDHPEEVVAITFTVKAAEEMRLRAIDAIRDAGQGEPKKDEHHRVTQEAARKVL
                     ERDRAREWKLLESPGRMRIQTLDALNAGIARSLPFSSGLGSGGQIVADAELQQLYRRS
                     AAATLDWLATDGPENASVEQVLSHLDNNTTAYIEHLARMLAKRDQWLPMSGAGFGKPG
                     EHEAARRKLERNIAEVVTARLARVRRLLPDEHLPALTNLCRYAAGNLAADEHEVARLA
                     ECNRFPEANAEQVGLWRAVASLLLTQSGSWRKSVNKSIGFPPRDEGQKQQMLELLSLL
                     EEQSSLAAELHRVRELPDPHYTGEQWDVLLALLQLLPLAVAELKTLFNACGATDHTEV
                     AVAADAALGNADEPGDIGLLLDFTIRHLLIDEMQDTSIAQYRLIEKLVSGWQPGDGRT
                     LFCVGDPMQSVYRFRDAEVGQFLLARERGIGPVSLEPLLLRQNFRSGEHLVHWYNSVF
                     GELFPPEDDVTAGAIAYSASVPVESRRGVGARHLYPLFGASNEEEADVAADVIRRCVD
                     VSEQETTAVLVRSRAQLPLLLGKLRESRIAYQAIEIDRLTDLPEIIDLLALTRALAHR
                     GDRIAWLGLLRGPWVGLCWTDLHTLVFNDKRSTVWELLRDEERLRALSAGGRESLQKF
                     GNTLAPFVSGHGTTFLRETVERAWYLLRGPLLLDNEEEVENVYRFLDVLDGLDTAGTI
                     QDVAELEAKLDQEHVSGKAASDCRLHIMTMHKAKGLQFDHIVLYGLGRMTKGDEKSIL
                     SWLNLPDAGDGPAMLLSPVGARAELETDRLHNFIEAAEREKGRLEQDRLLYVACTRAR
                     HSVHLVGHVDLTADGSEICEPRKGTLLHSLWPSTSRSFAEAFRNRSPESSGGSARHDD
                     AFRLPLLRRLDWDLALGTPPALPGARPGAGNAAPEPQVDFYWVGSVARHAGTILHRWL
                     HKLTSGLLEADVARLERLLPVARTHAKELGVPVQNIDDVCRRALDALRGILGD"
     gene            complement(3065..>3554)
                     /locus_tag="VFY27_02230"
     CDS             complement(3065..>3554)
                     /locus_tag="VFY27_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5764357.1"
                     /translation="REFLAAEMRRQAFAVVAVEKSVRLMRHGIDLDLKIDRIDRLKDE
                     TLLIADYKTGLVKALLDKDGEPKELQLVVYAAAVNEPVGGLVLINVDGRDIIYRGAGA
                     EWDRSPPGQWQGRLDDWMRSVDDALAGIAAGDVRLNTALPTDDGRPLAVLSRVEAVRR
                     GR"
BASE COUNT          635 a         1192 c         1053 g          674 t
ORIGIN      
        1 gtcaccgagt atcccacgca aggcatcgag cgcgcggcgg cagacatcat cgatgttttg
       61 caccggtact ccgagttcct tcgcatgggt acgggcaacc ggaagcagcc gctccagccg
      121 cgccacatct gcctccagca agcccgacgt caacttgtgc agccagcggt gcaggatggt
      181 tccggcatgg cgagcgacgg agccgaccca gtaaaaatca acctgcggtt ccggcgcggc
      241 attcccggcg cccggccgcg ccccgggcag cgcgggaggc gtacccagcg cgagatccca
      301 atcgagccgt cgcagcagag gcagtctgaa agcgtcgtca tgccgcgcgg aaccgcctga
      361 gctttccgga gatctgttcc tgaatgcttc cgcaaagctt cggctcgtcg acggccagag
      421 gctgtgcaac agcgttccct ttcgcggttc gcaaatttcg ctgccgtccg cggtcaggtc
      481 cacatgcccg accaggtgta cggagtgccg cgcgcgcgtg caggccacgt acaggaggcg
      541 gtcctgttcc agcctgccct tctcacgttc ggccgcttct atgaagttgt gcaaccgatc
      601 ggtctccagc tctgcgcgag ctcccaccgg actgaggagc attgcggggc cgtccccggc
      661 gtcgggcagg ttcagccagc tcaggatcga tttttcgtca cccttggtca tcctgccgag
      721 accgtacaag actatgtgat cgaactggag ccccttggcc ttgtgcatgg tcatgatgtg
      781 caggcgacag tcggaggcgg ccttccctga cacgtgctcc tggtcgagct tcgcttcaag
      841 ttccgcgaca tcctggatcg tgcccgcggt gtcgagccca tcgagaacgt cgagaaagcg
      901 gtaaacgttc tcgacttcct cttcgttgtc cagcaacaat gggccgcgca gcaggtacca
      961 cgcccgctcc accgtctccc ggaggaacgt ggtgccgtgt ccggagacga acggcgccag
     1021 ggtatttccg aatttctgca acgactcccg tcctcctgct gagagtgccc gcaagcgctc
     1081 ctcgtcgcgc agcagttccc acacggtgct gcgcttgtcg ttgaatacga gggtgtgcag
     1141 atcggtccag cacagcccga cccagggtcc gcggaggagg ccgagccagg ctatacggtc
     1201 gccacgatgc gcaagcgcgc gagtcagtgc gagcaggtcg atgatctccg gcaggtcggt
     1261 aagccggtcg atctcgatag cctggtatgc aatgcgactt tcgcgcaatt tcccgagcag
     1321 caacggcaat tgcgcgcggc tgcgtaccag caccgcagtc gtttcttgtt cagagacgtc
     1381 gacgcaacgg cggatgacat cggcagcaac atccgcctct tcttcattgc tcgcgccgaa
     1441 cagcgggtac aggtgtcgcg cacccacccc tcgacggctc tccacgggca cagatgccga
     1501 ataggcaatc gcgccggcag taacatcgtc ttctggaggg aacagctccc cgaacacgct
     1561 gttgtaccag tgcacgaggt gctcacccga acgaaaattc tggcgaagca acaacggctc
     1621 caggcttacg gggccgatgc cgcgctcgcg ggcaagaagg aactgcccga cttcagcatc
     1681 gcggaaccgg tacacggatt gcatggggtc gccgacgcag aagagcgtgc ggccgtctcc
     1741 cggctgccat ccggagacca gcttctcgat cagtcggtat tgcgcgatcg aggtatcctg
     1801 catctcgtcg atcaacaggt gccggatcgt gaagtcgagc agcaagccga tgtcaccggg
     1861 ctcatccgcg ttgcccaatg ccgcgtcagc ggccaccgct acctcggtat gatcggtagc
     1921 gccgcacgcg ttgaacagcg tcttcagctc cgccaccgca agtggcagca actgcagcag
     1981 ggcgagcaat acgtcccact gttcgccggt gtagtgcggg tccggcaact cccttacgcg
     2041 atgcagctct gccgccaggg acgactgctc ttccagcagg ctcagcaact cgagcatctg
     2101 ctgcttctgt ccttcgtccc gtggcggaaa gccgatgctc ttgtttaccg attttcgcca
     2161 gctgcccgac tgcgtcaaaa gcagggaagc gacagctcgc cacaggccga cctgttcggc
     2221 attcgcctcc gggaaccggt tacattcagc aagtcttgcc acttcatgtt catctgccgc
     2281 caggttcccc gcagcataac ggcagaggtt cgtgagcgcg ggcaggtgct cgtccggcag
     2341 caggcggcgc acgcgagcaa gccttgcggt caccacttcg gcgatgtttc gctcgagctt
     2401 cctgcgcgcg gcttcatgct cgccaggctt cccgaacccg gcgccgctca tcggcagcca
     2461 ttgatcgcgc ttcgccagca tacgcgcgag gtgttcaata taggccgtgg tgttattgtc
     2521 gaggtgagac agcacctgct cgacgctggc attctcaggg ccatctgtgg cgagccagtc
     2581 caacgtggca gcggctgacc tgcgatacaa ctgttgcagt tcagcatccg ctactatctg
     2641 gccgccgctg ccgagtcccg aactgaaggg aagcgaacgc gcgatgcctg cgttcagcgc
     2701 gtcgagtgtc tggatgcgca ttcttccagg cgactcgagc agcttccact ctcgcgcccg
     2761 gtcccgctcg aggaccttcc gcgcagcctc ctgcgtcacc ctgtggtgct cgtccttctt
     2821 cggctcgccc tgccccgcat cgcgaatggc atcgatggca cgaaggcgca tttcctctgc
     2881 agctttgacc gtgaacgtga tcgccacgac ttcttcggga tgatcgactt cagccaggag
     2941 cttcaggtag cgctggatca gtaactcggt ttttcctgaa cccgccggcg cctggacgat
     3001 gaacgaacgc gcgacgtcga gcgcatcggc acgagcattg agatctgcgg caagcaggtt
     3061 accgtcagcg cccacgtctg accgcctcca cccggctcaa gacagcgagc ggccggccgt
     3121 catctgtcgg caaggccgtg ttcagccgta cgtctcccgc tgcgataccc gccagcgcat
     3181 cgtccacgct ccgcatccag tcatccagcc gcccttgcca ctgccccggt ggcgaccgat
     3241 cccactccgc gccggcgccg cggtaaatga tgtcccggcc gtcgacgttg atcagcacca
     3301 ggccgcccac gggttcattc actgctgcgg catacacgac cagctgcagt tccttcggct
     3361 cgccgtcctt gtccagcaac gctttgacaa gacccgtctt gtaatcggcg atcagcaaag
     3421 tttcgtcctt gaggcggtcg atcctgtcga tcttcaggtc gagatcgatg ccatggcgca
     3481 tcagccggac ggatttctcg acagcaacga ccgcaaatgc ctgccggcgc atttccgcgg
     3541 cgaggaattc gcgc
//
LOCUS       DASUOM010000112         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_551049,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000112 DASUOM010000000
VERSION     DASUOM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551049"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFY27_02235"
     CDS             <1..364
                     /locus_tag="VFY27_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5764358.1"
                     /translation="GSIVNNSSGWGYSGGSRAIAYCAAKGGLIAMTKAMAIDHGRQGV
                     RVNCICTGDTVTPMEREDARNQGMTWEAYVEAASDRPLGRMGEPIEIARAALFLASEE
                     ASFITGAVLPVDGGGVAG"
     gene            461..1225
                     /locus_tag="VFY27_02240"
     CDS             461..1225
                     /locus_tag="VFY27_02240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5764359.1"
                     /translation="MRGLRGRNVVVTGGASGIGQATAQRFLEEGCAVCVVDRSAEARA
                     RVERELPKLTAAIAADVSSFEQVQAAFSEAIDRMGSVDVLINNAGISIRHDFLDITPE
                     EWDEVLGVNLRGVFYMAQTAARHMIDRGSGVILNTASTGGSTGYPHYADYSASKGGVI
                     ALTLAMALELAPTVRVNAISPGYVLTPMQRAEYTDAMLDAVNRKIPLGRHAKPQEIAA
                     LFAFLASEDGAFASGQVYVMDGAETTGGLCSQWSHN"
     gene            1314..2273
                     /locus_tag="VFY27_02245"
     CDS             1314..2273
                     /locus_tag="VFY27_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764360.1"
                     /translation="MPEILELDAIRSQIDVPALIRAIEQGFVLYSENKVVVPPVGFLH
                     FEQPPGDVHIKYGYIKNDDVYVLKVASGFYDNAKLGLPVADGVILVFSQQTGHLERVL
                     HDECWLTDMRTAAAGAVAAKHLAPRTVRHIGIVGTGTQARMQLEFLRGVIDCQSCVVW
                     GRDPDKVHQMIEDLASRNGIREWGLELKAAGSVDELAAECNLIVTTTSARQPVLRADQ
                     VRAGTHITAMGSDDHGKQELEAALLGKADLVVADSISQCVDHGECHYAVQNGLIDEAS
                     IVELGNVIKYPEIGRASDDQITIADLTGIAVQDIQVAKLVGVG"
     gene            2309..2740
                     /locus_tag="VFY27_02250"
     CDS             2309..2740
                     /locus_tag="VFY27_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764361.1"
                     /translation="MKKLRILLPFILSLLWQSGAVALEVSATVKVSTLLKTESSWNGK
                     PIEYPSGKAEVTGMVVEIAPGGETGWHSHPVPSFGFVLEGELEVRLRSGAVKRVKPGE
                     ALAEVVNTFHNGRNVGSVPLRLVVFYVGSAGQKLSVNESRQ"
     gene            3060..>3405
                     /locus_tag="VFY27_02255"
     CDS             3060..>3405
                     /locus_tag="VFY27_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764362.1"
                     /translation="MATTPGLSRRSVLVSGLAAGAGLLLPAARAFGLAAGADDRSGPG
                     EPPAKDTSTDLLIIGAGPFGLALAAYAQEQGIRHTVVGVPMGFWKGNMPEGMLLRSTC
                     DWSLDPLAVHSID"
BASE COUNT          707 a          948 c         1059 g          691 t
ORIGIN      
        1 cggctcgatc gtcaacaaca gctctggctg gggctatagc ggtggctccc gtgcgatagc
       61 ctattgtgcc gccaaaggcg gtctgattgc gatgactaag gcaatggcga tcgatcacgg
      121 ccggcagggt gttcgcgtca actgcatctg taccggcgac acggtgacgc caatggagcg
      181 cgaagatgcc aggaatcagg gcatgacctg ggaggcgtat gttgaggctg cctccgatcg
      241 accactcggc cgcatgggcg agccgatcga gattgcgcgt gccgccctgt ttctcgcttc
      301 ggaggaggcc tcgttcatta ccggcgctgt tctgccggtg gacggcggcg gcgtggccgg
      361 ttgagcaggt ccggcgtgtg aagcagccgg gtcgctctgc cgggtaacca tctgcgggat
      421 gcgcagaata tatcgtgtag cgactgactc gaggtgagca atgcgaggtt tgaggggaag
      481 aaacgtggtt gtaaccgggg gcgcgagtgg cattggccag gctacagcgc agcgcttcct
      541 cgaagagggc tgcgccgttt gtgtcgtcga ccgcagtgcc gaggcacgag cgagagtcga
      601 gcgagaactg ccgaagctga cggcagcaat tgccgccgac gtctcgagct tcgagcaggt
      661 tcaggcagcc ttctccgagg ccatcgaccg catgggatcg gtcgacgtat tgatcaataa
      721 cgccggcatc agcatacggc acgatttcct cgatatcacg ccggaggagt gggacgaggt
      781 gctcggcgta aacctccggg gtgtcttcta tatggctcag accgccgcac gtcacatgat
      841 cgaccggggc tccggggtca tcctgaacac agcttccacc ggaggatcca cggggtaccc
      901 tcactatgcc gactacagcg ccagcaaagg gggcgtgatt gcgctcacac tggcaatggc
      961 gctcgaactg gcgcccaccg tccgcgtcaa cgcgatttcc cccggttacg tgctcacgcc
     1021 gatgcaacgt gccgagtata cggacgctat gctcgacgct gtgaaccgca agattccgct
     1081 tggcaggcac gccaagccgc aggaaatcgc tgccttgttc gctttcctgg cctcggaaga
     1141 cggtgcgttc gcgagcggac aggtctatgt gatggacggc gcagaaacca caggtggatt
     1201 gtgcagccaa tggtcacaca actagccagc agtgccagaa tttgcccggc ccgtagtgtt
     1261 tggtcgggat atgacgtcca cgagcgtgtc cagtttatgt aagagaacgc gtgatgccgg
     1321 aaatactcga gcttgacgcc atcagaagcc agatcgacgt tccagctttg atccgagcga
     1381 tcgaacaggg ttttgtcctg tactctgaaa acaaagtcgt cgtcccgccg gttggtttcc
     1441 tccacttcga acagccgcct ggcgacgtgc acatcaagta cgggtatatc aagaatgacg
     1501 atgtctatgt tctcaaagta gcatccggtt tctacgacaa tgccaagctc gggttgcctg
     1561 tcgcggacgg cgtaatcctc gtgttcagcc agcaaacagg ccacctcgag cgggttctcc
     1621 acgacgaatg ctggttgacc gatatgcgta ccgccgcggc tggcgccgtt gccgcgaagc
     1681 acctcgcacc caggactgtt cgtcacatcg gaattgtcgg cacgggtacc caggcgcgaa
     1741 tgcagctcga gttcctacgg ggcgtcattg actgtcagag ctgtgttgtc tggggccgag
     1801 atcccgacaa ggtacaccaa atgatcgagg atcttgcgtc caggaatggt atccgggagt
     1861 gggggctcga gctcaaggcg gcaggcagcg ttgacgaact tgctgcggaa tgcaatctca
     1921 tcgttaccac gacgtcggcg cggcaacccg tgctccgcgc ggatcaggta cgagcgggca
     1981 cacacatcac ggccatgggt tccgacgacc acggcaaaca ggagctggag gcagcactcc
     2041 tcggtaaggc cgatctggta gtagcggaca gtatctcgca atgcgtggat catggcgagt
     2101 gccactatgc agtgcaaaac ggactgatcg atgaggcatc aattgttgaa ctggggaacg
     2161 tcatcaagta tcccgaaatc ggccgagcga gcgacgacca gattacgatt gcagatttga
     2221 cgggcattgc tgtacaggat atccaggtag ccaagctggt aggcgtcggc tgagaaaaca
     2281 cagtcccaac agatgggggg aacccaccat gaagaaattg agaatactcc ttccgttcat
     2341 tctgtctttg ttgtggcagt ccggggcggt ggccctggaa gtctccgcca cagttaaggt
     2401 ttcaacttta ttgaagaccg aatccagttg gaatggaaag ccaatcgaat atccatcggg
     2461 taaagctgaa gttactggta tggttgttga aattgctcct ggtggtgaga caggttggca
     2521 ctcacaccct gtcccttcat tcggtttcgt tctggaaggt gagctggaag ttcgactaag
     2581 aagtggcgcc gtaaaacggg tgaaaccagg tgaagcgctg gctgaggttg taaatacatt
     2641 ccataatggc cgtaatgtcg gctcagtgcc cctgagactc gttgttttct atgtgggttc
     2701 agccggacaa aagttatcag tgaatgagag ccggcagtag tctggtcgtg acacagaggc
     2761 gcgtacccgc ctgtacgtca ccttgtcgag gcgccccgtg aggtcagcat cagcgccgag
     2821 tggagctcga actctcgcgc cgggatccca acctgaatac gggcgacagc attaagacga
     2881 caacgacgtg gcgcgaaaca ccgcgcacca gtcgccggag cgaccttcgt tcattgtcct
     2941 gtccatagtc gactgagatc atctcccgct ggaaaattcc tgggattctc gggcgtcgcg
     3001 acgcattgcg accggcttca cgattgcaac gatgtatact gcgaaagggg gaacgcgaca
     3061 tggccacaac gcctggactt tctcgacgta gcgtgctcgt ttccggactc gccgcgggtg
     3121 ccgggctgct cctgcccgct gcacgtgcgt tcggtcttgc tgctggcgct gatgaccgga
     3181 gcgggccggg cgagccgccg gcgaaagaca cctctaccga tctcctcatc ataggcgccg
     3241 gacccttcgg tctggccctg gctgcctatg ctcaggaaca gggtattcgg cacacggtcg
     3301 tcggcgttcc aatgggcttc tggaagggca acatgccgga aggaatgctt ctgcgctcga
     3361 cctgcgactg gtctttggac ccactggctg tccattcgat cgacg
//
LOCUS       DASUOM010000113         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1601726,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000113 DASUOM010000000
VERSION     DASUOM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601726"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..777)
                     /locus_tag="VFY27_02260"
     CDS             complement(154..777)
                     /locus_tag="VFY27_02260"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5764363.1"
                     /translation="MDELADEAPVLHLASASPRRREILAAIGVVHTWEGVDVDETPLP
                     GEPAGALATRLALAKSRAGRQTRTAEPVILGADTVVELDGVTFGKAGSEEEALFMLSR
                     LSGKVHRVFTAVTLDTPAGERTALSRTEVRMRKIAPDEALSYWRSGEPRGKAGAYAIQ
                     GIGGIFIEALSGSYSGVVGLPVFETALLLRQAGIELLRRVSTTGGAR"
     gene            complement(782..1252)
                     /gene="rlmH"
                     /locus_tag="VFY27_02265"
     CDS             complement(782..1252)
                     /gene="rlmH"
                     /locus_tag="VFY27_02265"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5764364.1"
                     /translation="MHIRLIAVSDRQPAWVGDAFGEYVKRLPEQWRFRLDTVPTARRT
                     GAGSASQATEAEGLLVLKQLKPVETVILLDEAGTELTSEGLSSRLAGWQAAGSDLAFI
                     IGGPDGISADCARRADFRWSLSRLTLPHGLARVVLAEQLYRAHSLLQGHPYHRA"
     gene            complement(1263..1616)
                     /gene="rsfS"
                     /locus_tag="VFY27_02270"
     CDS             complement(1263..1616)
                     /gene="rsfS"
                     /locus_tag="VFY27_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5764365.1"
                     /translation="MNSEQLSDLAVEALEEVKAKDVIKLDVRNLTTVTDFMIVASGTS
                     NRHVKALADAVSDKAREAGHRPTGVEGTEGSEWILLDLQDTLVHVMLPRVREFYNLEK
                     LWSLAPASDLAARDR"
     gene            complement(1683..2372)
                     /gene="nadD"
                     /locus_tag="VFY27_02275"
     CDS             complement(1683..2372)
                     /gene="nadD"
                     /locus_tag="VFY27_02275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5764366.1"
                     /translation="MGPMGIFGGTFDPIHFGHLRSAFEMLQALRFDEVRFMPCGSPPH
                     RGSPVADPALRLRMVRVATEGQQGFVVDDRELQRDGPSYSVDTLTALRSEFPLTPLAL
                     MIGMDAFLGLPKWYHWREILQLSHIVVAHRPGWRAPDMGPLGELLTDRGTHRIGDLHQ
                     ARSGHIYIHDVTQLEISSTEIRELVGAGRDPRYLMPDSVRAVIQESGCYGSAGGDSGK
                     SVGNATKKAAG"
     gene            complement(2413..>3198)
                     /gene="holA"
                     /locus_tag="VFY27_02280"
     CDS             complement(2413..>3198)
                     /gene="holA"
                     /locus_tag="VFY27_02280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5764367.1"
                     /translation="ILELKVPTGKPGRDGSAAIADLMQQVGDDLMVLVEAPRLDKSAS
                     SKWVKEIEAVGAHVQVWPVGPRELPGWIAARARAVGLKPDRDAVNLLVDRVEGNLLAA
                     HQEIEKLRLLLGDGPVSGADVENAVADSSRFDVYKLTDAAVEGKAARAIRMLGRLRAE
                     GVEPVIVVWALTRELRTLARVAEMVQTGSDLGAALQRAGVWRTRQASVRSCVARHAAS
                     DFYRLLQITRRADAAAKGQMRVDPWQLVTEIVFGLAAAATKAA"
BASE COUNT          539 a         1120 c          928 g          611 t
ORIGIN      
        1 gcgatacccg gcccttatag atgttgctgt tcaagccgcg ccgcgcggcg cgctctatgt
       61 agacttcctg caggaccccg ttttcgagca gcgccgcgcg aacctcccgg ggagtcacgt
      121 tgaccagtat ttcttctttc ggcagttgct cgctcatcgt gctccccctg tcgtgctgac
      181 ccggcgcaac aactcgatgc cggcctgcct gagcagcaac gccgtttcga aaaccggcaa
      241 gccgaccacc ccggaataac tgcccgacag cgcctcgatg aagattccgc caattccctg
      301 tattgcgtac gcgcccgcct tgcctctcgg ttctccgctc cgccagtaac tcaacgcttc
      361 atcgggcgcg atctttcgca tccgcacctc ggttcgcgac aaggccgttc tttcgcccgc
      421 cggggtatcg agggtgacgg cggtaaacac ccggtgcacc tttcccgaga gccgggacaa
      481 catgaacagc gcttcttcct ccgaaccagc cttgccgaaa gtgaccccgt cgagctccac
      541 cacggtgtcg gcacccagta ttacaggttc cgcggtgcgg gtttgccggc ctgcccttga
      601 ctttgccagc gccagccggg tggccagtgc gcctgccggc tcacctggaa gaggtgtctc
      661 atcgacgtcg acaccttccc aggtatgcac cacgccgata gccgccagta tctcccgccg
      721 ccgcggggac gcggaagcga ggtgcagaac cggcgcctca tccgccaact catccattga
      781 gtcatgcacg atggtaaggg tgtccctgca acaggctatg ggcgcggtaa agctgctcgg
      841 ccagcaccac tcgcgccagt ccgtgcggca gagtcaggcg ggagagtgac catctgaaat
      901 cggcgcggcg ggcgcaatcg gctgagattc cgtccgggcc gccgataata aaggccaggt
      961 cgctgcctgc tgcctgccag ccggcgagcc gggaggacag gccttcgctc gtcagctccg
     1021 tgccggcttc atcgagaagt attaccgtct ccacgggctt caactgtttc aggaccaaaa
     1081 gtccttcggc ctctgttgcc tggcttgcgc tgcctgcccc tgtccggcgt gcggtgggaa
     1141 cggtgtcgag ccggaagcgc cactgttccg gaaggcgctt tacgtactcg ccgaatgcgt
     1201 ctcccaccca ggcaggctgc cggtcgctca cggcaatcag ccggatgtgc atgcgccgtg
     1261 cgctatctat ccctggcagc gaggtcgctc gcgggtgcca gcgaccacag tttttccagg
     1321 ttgtagaatt cacggacccg cggcagcatc acgtgcacga gagtgtcctg aaggtccagg
     1381 agtatccatt cgctcccttc cgtgccctcg acgccggttg ggcggtgtcc ggcctcgcga
     1441 gccttgtcgg aaaccgcgtc agccagagcc ttgacgtgcc ggttcgaggt gccgctcgcc
     1501 acgatcatga agtcggtcac cgtggtcagg ttgcgcacgt cgagttttat tacgtccttc
     1561 gcctttactt cttccagtgc ctcgaccgcc aggtcgctca actgctcgct attcattcat
     1621 cgatccgctt ttcggtgcca atattttgct ccttgatttg aaaatccgcc tgatggcttc
     1681 gtttaccctg ctgctttctt cgttgcgttc ccgacgctct tgccggagtc accgccagcc
     1741 gacccgtaac agccgctttc ctggatcacc gccctgacgc tgtcgggcat caggtaccgc
     1801 gggtcccgcc cggctccgac cagctcacga atctccgtcg atgaaatctc gagctgcgtc
     1861 acgtcgtgga tgtaaatatg accggagcgg gcctggtgca ggtcaccgat gcgatgcgtg
     1921 ccgcggtccg tgagcagttc ccccagtggg cccatgtcgg gcgcgcgcca acccgggcga
     1981 tgcgcaacga caatgtgcga caactgcagg atttcgcgcc agtgatacca cttcggcaac
     2041 ccgagaaaag cgtccatgcc gatcatcagc gcgaggggcg tgagcgggaa ttcggagcgc
     2101 agcgccgtca gggtatccac cgagtacgac gggccatcac gctgcaattc gcgatcgtct
     2161 acgacgaaac cctgctgccc ctccgtcgca acccggacca tcctgagccg cagagcggga
     2221 tccgcgaccg gcgagccgcg gtgcggcgga ctgccgcatg gcatgaaacg cacttcatcg
     2281 aaccgcagcg cctgcaacat ctcgaaggca gaacgcaggt gtccgaaatg tatcggatcg
     2341 aaagtcccgc caaaaattcc catcggaccc atgtctttca cgtctcttaa cgttgcttga
     2401 gcagagcctc cctcaggctg ccttcgtggc ggcggcggca aggccgaaca cgatctccgt
     2461 caccagctgc cacgggtcga cccgcatctg gcccttggcg gcagcatctg cgcggcgggt
     2521 gatttgcagc aagcggtaga aatcggacgc ggcgtgccgc gcgacgcaac tccttacgct
     2581 tgcctgccgc gtgcgccaga cgcccgcacg ctgcaatgcc gcgccaaggt cactgccggt
     2641 ctgcaccatc tcggccaccc tcgcgagcgt gcgcaactca cgcgtcaggg cccagacgac
     2701 gattaccggc tccacgcctt ctgcccggag cctgccgagc atccggatcg cacgtgccgc
     2761 tttgccttca acggcggcgt ccgtcagctt gtaaacatcg aagcggctgc tgtctgcgac
     2821 ggcgttctcc acgtccgccc cgctaacggg tccgtcgccg agcaacaggc gcagtttttc
     2881 gatctcctgg tgcgcggcaa ggaggtttcc ctcaacgcga tccaccaaca gattgacggc
     2941 gtcccggtcc ggcttcaggc ccaccgcgcg cgcgcgcgcc gcgatccagc ccggcagttc
     3001 gcgcggtccg accggccaca cctgcacgtg ggctcccacc gcctcgatct ccttcaccca
     3061 cttcgaggac gcactcttgt ccagcctcgg cgcctcgacc agcaccatca ggtcgtcccc
     3121 tacctgttgc atgagatcag cgatcgccgc cgacccgtca cggcccggct tgccggtcgg
     3181 caccttgagt tcgaggat
//
LOCUS       DASUOM010000114         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_627895,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000114 DASUOM010000000
VERSION     DASUOM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627895"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFY27_02285"
     CDS             <1..736
                     /locus_tag="VFY27_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX5764368.1"
                     /translation="FMEIAPENWIHVGGKLGRKLRFFTERYPFLLHGLSLSIGGPAPL
                     DESLLQNIKAFMDEHDIQSYSEHLSYCGDDGHLYDLMPIPFTEEAVAYVAARVRRAQD
                     ILERRIALENVSYYAPTDSSLSEVEFTTAVLEEADCDLLLDINNIVVNSINHNYDVRE
                     FMLAMPRHRVRYFHQAGHYVESEDLRVDTHGAAVSDAAWALLAQAYNHFGPLPTLLER
                     DFNFPPIDDLLSEVRRIRSMQAGSHD"
     gene            729..1556
                     /locus_tag="VFY27_02290"
     CDS             729..1556
                     /locus_tag="VFY27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEX5764369.1"
                     /translation="MTERDEPESNAIGTSRFQRRQLSLAAHIRDPENNPAPEDIEDRR
                     LAIYRELFANNLGNLLGSSFPVLRKLHGAEKWKSLVRDFLVRHRAHTPYFLEIPREFL
                     DYLEYTRGDREGDYPFLLELAHYEWAELALSVSDAKNDLSGIDPDGDLLDGVPVKSVL
                     AWALSYNFPVHRISPEFIPREAGQQPTHLVVFRKADEELEFRELNPVTARLLHLIGDD
                     GRDSGRSMLSLIASEIGFGEEAMMQHGGVILRQMRADGILVGSRAPPSQEQQKGHTT"
     gene            1553..2095
                     /locus_tag="VFY27_02295"
     CDS             1553..2095
                     /locus_tag="VFY27_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5764370.1"
                     /translation="MNRLRALWESVTAALAGAGQWLSLLPLRLILAWEFWEAGVEKLA
                     GENWFDSVRADFPFPFNVISADTSWFLATWTELLGGLGLALGLFTRFWSAGLIVLSIV
                     AIAGVHWPAEWNSLAELWQGYSVSDRGFGNYKLPLLFIVMLLPLVFFGAGKASLDHVL
                     LRWLSLRKPSLTTGEALRSA"
     gene            complement(2265..3686)
                     /gene="lpdA"
                     /locus_tag="VFY27_02300"
     CDS             complement(2265..3686)
                     /gene="lpdA"
                     /locus_tag="VFY27_02300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5764371.1"
                     /translation="MASTHSTELVVIGAGPGGYTAAFRAADLGMRVMLVERWPMLGGV
                     CLNVGCIPSKAFLHAAKVIDDAAAMSANGIHFGKPRIDAGKLAAWKSQVVGRLTGGLT
                     QLARQRKVEVVHGTAKFDTPTRLMLDNGETVDFRQCIIAAGSESAKLPGLPDDPRIVD
                     STGALELAPLPKRLLVVGGGIIGLEMACVYNALGSKVSVVELTDALMPGTDPDLVRPF
                     RKHVDAHYEAIMLETRVTGMRPTVPGIEVKFEGKGAPESELFDRVLVAVGRRANGDKL
                     DAEKIGIEITKGGIIKVDKQMRTSVAHIFAIGDLAGGPMLAHKASHEGKVAAEVASGE
                     KVAFDARAIPSVAYTDPEIAWVGLTETDAKRDGVEYEKTKIPWSASGRALALGRPEGF
                     TKLLFDKKTRQVLGGGIVGPSAGDLISEVSLAIEMGADAADVSLTIHPHPTLSETIGF
                     AAEAHEGTLTDLYMPVKASRKAT"
     gene            complement(3686..>5336)
                     /gene="aceF"
                     /locus_tag="VFY27_02305"
     CDS             complement(3686..>5336)
                     /gene="aceF"
                     /locus_tag="VFY27_02305"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX5764372.1"
                     /translation="RVSREDGLITLETDKAAMDVPAEEEGEIEEISVSVGDKVSTGDV
                     IGRLKTGAAADGDDDDGKDAGDTDDVDEKADEATARTGEGGGGQDESGSGDEQEGEPE
                     DKSGGKAEQKPGARQTVTVPDLGDFSDVEIIEVHVKPGDEIKLEDGLISLETDKAAMD
                     VPSTVAGRVESVLVKVGDRVSQGSEIAVIEAAGDAGGAKPAGPGKKQADTGPAATREP
                     EEKTPKKESAPKPAARPEGPARKLPPIDEAGFARAHASPSVRKLARELGVDLASVGGS
                     GEKNRVLHDDVKAFVKDILGRGGPGAALPKVPSIDFSSFGEIERQPLTRIQKIAGPRL
                     QASWINLPHVTQHDLADITGLEEKRQELKAAAKERGINLTVLAFIMRACVATLLEYPK
                     ANASLAEDGENLVYKKYIHLGFAADTENGLMVPVIRDADKKDVFEIATELGDLSKAAR
                     EGKLKASQMQGASFTISSLGGIGGTAFTPIVNAPEVAILGVSRSTMQPVWDGSEFRPR
                     LMLPLSLSYDHRVIDGAYAVRFTTFLSKTLSDVEALLEAIP"
BASE COUNT          885 a         1736 c         1602 g         1113 t
ORIGIN      
        1 cttcatggag atcgctccgg agaactggat tcacgtcggc ggcaagctcg gcaggaagtt
       61 gcgctttttt accgagcgct atcccttcct tctgcacgga ctgtcgctca gcatcggtgg
      121 ccccgcgccg ttggatgaat cgctgctaca gaacatcaag gccttcatgg acgagcacga
      181 catccagtcg tattccgagc acctgagcta ctgcggggat gacgggcacc tgtacgacct
      241 gatgccgatt cccttcactg aggaggcggt cgcttacgtc gccgcccgcg ttcggcgcgc
      301 gcaagacatt ctggaacggc gcatcgcgct cgaaaacgtg tcttactacg ccccaacaga
      361 cagttccctc agcgaggtcg agttcaccac ggctgtactc gaggaagcag attgcgactt
      421 gctgctcgac atcaacaaca tcgtcgtcaa cagcatcaac cacaattacg acgttcgcga
      481 attcatgctc gccatgccca ggcacagggt ccgctacttt caccaggccg gccactacgt
      541 ggaatcggag gatcttcgcg tcgacaccca cggtgcggcg gtcagtgacg cggcgtgggc
      601 cctgctcgcg caggcttata accacttcgg gcctttgccg accttgctgg agcgggactt
      661 caacttcccg cccatcgacg atctgttgtc ggaggtccgg cgtatcagat cgatgcaggc
      721 gggttcgcat gactgaacga gacgagcctg aaagcaacgc catcggcacc agccggtttc
      781 aacgccggca actctccctg gcggcccaca ttcgggatcc ggagaacaat ccggcgccgg
      841 aggacatcga ggaccggcgt ctcgcgatct accgtgagtt gtttgccaat aacctcggga
      901 acctgctggg cagctccttc ccggtgctca ggaagctcca tggcgccgag aagtggaagt
      961 cattggtgcg cgactttctc gtgcggcatc gcgctcacac accatacttc ctcgagattc
     1021 cacgcgagtt tctggattac ctcgaataca cgcggggaga ccgggaaggt gattacccgt
     1081 tcctgctgga gctcgcgcat tacgagtggg cggaactcgc gctttcggtt tcggatgcga
     1141 agaacgacct gtccggcatt gaccccgacg gggacctgct cgacggcgtg ccggtcaaat
     1201 ccgtgctggc gtgggcgctg tcttacaatt tcccggtgca ccggatttca cccgaattca
     1261 ttccgcgcga ggcgggtcaa cagccgacgc acctcgtggt cttccggaag gccgatgaag
     1321 aactcgaatt ccgcgaactg aatcccgtca ctgcgcggct gctccacctg atcggggatg
     1381 acgggcgcga ctccgggcgc agtatgttgt cgctcattgc atccgagatc ggcttcggcg
     1441 aagaggcaat gatgcagcac ggcggagtga tactgaggca gatgcgcgca gacggaatac
     1501 ttgtcggcag ccgagcgcca ccctcacaag agcaacaaaa aggacacacg acatgaaccg
     1561 gttacgcgcc ctgtgggaat ccgtgacggc ggcgctggca ggagccgggc agtggctttc
     1621 gctcctgccg ttgcggctga tcctggcatg ggagttctgg gaggcgggcg tggaaaagct
     1681 cgcgggcgag aactggttcg acagcgtgcg cgccgatttt cccttcccct tcaacgtcat
     1741 ttcggccgac acaagctggt ttcttgccac ctggaccgag ctgctcgggg gcctggggct
     1801 ggcgctggga cttttcacgc gcttctggtc tgcggggctg attgttcttt cgatcgtcgc
     1861 catagcgggt gtccactggc cggcggaatg gaacagcctc gcggaactgt ggcagggtta
     1921 ttcggtcagc gatcgcggtt tcggcaacta taagctgccg ttgctgttta tcgtgatgtt
     1981 gttgccgctc gtgttcttcg gcgccggtaa agcgagcctc gatcacgtgt tgctgcgatg
     2041 gctttcgcta cggaagccgt cgctgaccac cggcgaggcg ttgcgaagtg catgatgctc
     2101 tgtcgcccgc atggcgggct cctgctgcgc cctgcaatgg cgaatgttcg gaacaggaga
     2161 ctgtaggagc ccgccatgcg ggcgaatgcg attccgcacg ggaagtgcga attgttaacg
     2221 gcctcacact cgcccgcgtg gcgggctcct acgaggcctc gggttcaggt agctttgcgg
     2281 cttgccttca ccggcatgta caggtccgta agcgttccct cgtgtgcttc ggcggcgaag
     2341 ccgatcgtct ccgacagtgt cgggtgcggg tggatagtga ggctgacatc ggcggcgtct
     2401 gcgcccatct cgatggcaag gctcacttcc gagatgaggt cgccggccga cggcccgacg
     2461 atgccgccgc ccagcacctg ccgggttttc ttgtcgaaca ggagcttcgt gaagccctcc
     2521 ggacgtccca gcgcaagagc gcggccgctg gcgctccagg gaatcttggt cttttcgtat
     2581 tcgacaccgt cgcgtttggc gtcggtctcc gtcagcccca cccacgcaat ctccgggtcc
     2641 gtataggcaa cggagggaat ggcgcgcgca tcgaacgcga ccttctcccc tgaagccacc
     2701 tccgcggcga ctttcccttc gtgcgacgcc ttgtgcgcaa gcatggggcc gcccgccagg
     2761 tcgccgatgg cgaagatatg cgcaacgctc gtgcgcatct gcttgtccac cttgatgatg
     2821 ccgcccttgg tgatttcaat tccgatcttc tcggcatcga gcttgtcgcc gttcgcacgg
     2881 cgtcccacgg caaccagcac gcggtcgaac agttccgatt ccggcgcacc cttgccttcg
     2941 aattttacct cgatgcccgg aaccgtcggt cgcatgcccg taaccctggt ctcgagcatg
     3001 atcgcttcgt aatgcgcatc gacgtgtttg cggaaaggcc tgacgagatc agggtcagtc
     3061 ccaggcatca gcgcgtcggt cagttcaacg acgctgacct tgctgcccag cgcgttgtaa
     3121 acgcaggcca tctcgagccc gatgatgcca ccgccgacga ccagcaggcg tttcggaagc
     3181 ggcgcgagct ccagcgctcc ggtcgaatcg acgatgcgag gatcgtccgg aagccccgga
     3241 agcttcgcac tctcggagcc tgcggcgatg atgcactgcc tgaaatcgac ggtctccccg
     3301 ttgtccagca tgagccgcgt cggagtatcg aacttcgccg ttccgtgcac gacttccact
     3361 ttgcgttggc gggccagctg cgtaaggccg ccggtcaacc tgccgacaac ctggctcttc
     3421 cacgccgcaa gtttccccgc gtcgatcctt ggcttgccga agtgaatgcc gttcgcactc
     3481 atcgccgcgg catcgtcgat caccttggcg gcgtgcagga atgctttcga ggggatgcag
     3541 ccgacattga gacagacgcc gccgagcatc ggccagcgtt ccaccagcat gacccgcatg
     3601 ccgagatcgg ccgcgcggaa cgccgctgtg taacctcccg gaccggcgcc gattactacc
     3661 agctcggtgg agtgtgtcga agccatcagg gaattgcctc gagcagtgcc tcgacgtcgg
     3721 acagcgtttt actcaggaaa gtagtaaagc gtacggcgta tgcgccgtcg atgacgcgat
     3781 gatcgtaaga caacgacaac ggcaacatga gccgcggccg gaactcggaa ccgtcccaga
     3841 ccggctgcat cgtcgaacgg gacacgccga ggattgccac ttcaggcgca ttgacgatcg
     3901 gcgtgaatgc cgtgccgccg atgccgccca ggctcgagat cgtgaaactg gcgccctgca
     3961 tctggctggc tttcagcttg ccctcgcgcg cagccttcga aagatcgccc agttctgtcg
     4021 caatctcgaa cacgtctttc ttgtccgcgt cgcggatgac cggaaccatc agcccgttct
     4081 cggtatccgc agcgaacccc aggtgtatgt acttcttgta caccaggttt tctccatcct
     4141 cggcaaggga agcgttcgcc ttcgggtact cgaggagcgt cgccacgcag gcccgcatga
     4201 tgaacgccag cacggtgagg ttgatgccgc gttcttttgc cgcagctttc agctcctgtc
     4261 gcttctcttc caggccggtg atgtctgcga ggtcgtgctg agtcacgtgg ggcaggttga
     4321 tccagctggc ttgcagccgt ggtcccgcaa tcttctggat gcgcgtaagc ggctgccgct
     4381 cgatctcgcc gaagctcgag aagtcgatgc tcggaacctt cggcagcgcc gcgccgggac
     4441 cgccgcgacc caggatgtcc ttgacgaatg cctttacgtc gtcgtgcagt acccgattct
     4501 tctcgccgct gccgccgacg ctcgccaggt cgacgcccag ttccctggcg agcttgcgca
     4561 cggaggggct tgcgtgcgcc cgcgcaaaac ccgcctcgtc gatcgggggc agcttgcgcg
     4621 cgggcccttc cggtctcgct gcgggctttg gagcggactc cttcttcggc gttttttctt
     4681 ccggctccct tgttgccgct gggcctgtat cggcctgttt ctttcccggc ccggcaggct
     4741 tcgctcctcc agcgtcgccc gccgcttcga tcactgcaat ctccgatccc tgcgacacgc
     4801 ggtcgccgac tttcacaagc acggactcca cccggccagc cacggtggag ggtacgtcca
     4861 tcgcggcctt gtcggtttcc aggctaatga gaccgtcctc gagcttgatc tcgtctcccg
     4921 gcttcacgtg gacctcgatg atttcgacgt cgctgaagtc gccgaggtcc ggaaccgtca
     4981 ctgtctgcct ggcaccgggt ttctgttcgg ccttcccgcc ggacttgtct tccggctccc
     5041 cttcctgttc gtcgccggat ccggattcgt cttgtcctcc tcctccttcg ccagtccgtg
     5101 ccgttgcttc atccgctttt tcatcaacat cgtccgtatc gccggcatcc ttgccgtcgt
     5161 cgtcgtcacc gtctgcggcg gcaccggtct tcagccggcc gatgacatcg ccggtcgata
     5221 ccttgtcgcc gactgacacg ctgatctcct cgatctcgcc ctcttcctcc gccggcacgt
     5281 ccatggcggc cttgtcggtc tccagcgtga tcagcccgtc ttccctcgag acgcgg
//
LOCUS       DASUOM010000115         5201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_803561,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000115 DASUOM010000000
VERSION     DASUOM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803561"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /gene="glgX"
                     /locus_tag="VFY27_02310"
     CDS             complement(<1..2057)
                     /gene="glgX"
                     /locus_tag="VFY27_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5764373.1"
                     /translation="MNRKVSRVWLGKPYPLGATWDGEGVNFALFTEHADKVELCLYSE
                     HDEAQGQSVVLPEQTDQVWHCYLPDARPGQRYGYRVHGPWRPREGHRFNPAKLLLDPY
                     AKSIEKWVGWSDSHFAYRVGDKQADLAIDDRDNSADMPKCRVIDSAFTWGDDRPPRTP
                     WHETVIYELHVKGFTVQHPDVAPELRGTYAGLATPAIIEYLQELGVTAVELMPVHYAI
                     SDRHLVDKGLSNYWGYNTIGFFAPDVRYRSTDAVTEFKSMVKTLHSAGIEVILDVVYN
                     HTAEGNHLGPTLSFRGIDNLTYYRTEADRPRYYTDYTGCGNTLNMGNARTLQLIMDSL
                     RYWVLEMHVDGFRFDLAAALARELHDMSRLTAFLDTIHQDPVLSQVKLIAEPWDIGEG
                     GYQVGNFPPGWTEWNGRYRDHIRDYWRGEGGLISDLAYRLTGSSDLYARNGRRPVASI
                     NFITAHDGFTLRDLVSYNEKHNEANLENNQDGESHNRSWNCGAEGETDDTEVLTLRAR
                     QQRNFLATLFLSQGVPMLLAGDEVNRRQKGNNNVYCQDNELSWMNWEFGNEDRSLLAF
                     VQQLISLRKAHPVFRRRRFFQGQAIKGASVKDILWLDPAAVEMTEEEWASSHAKTLGV
                     LLPGEGIEELDARGDRILDDTFLVLMNAHHEPVPFTLPTLASGTRWLAVFDTARDGGV
                     KSQG"
     gene            2180..2314
                     /locus_tag="VFY27_02315"
     CDS             2180..2314
                     /locus_tag="VFY27_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764374.1"
                     /translation="MHAIAWRLGAEAETRFSWNVVAEGTLKLYESHLTAADGLVILLV
                     "
     gene            complement(2552..2785)
                     /locus_tag="VFY27_02320"
     CDS             complement(2552..2785)
                     /locus_tag="VFY27_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764375.1"
                     /translation="MGTYTALISSTLLLSTFPLSIVKFLALMWIGAVLSLLAILGIAI
                     FARWVILPCADPALHRLISASQRRYQPLGKNYR"
     gene            3040..3228
                     /locus_tag="VFY27_02325"
     CDS             3040..3228
                     /locus_tag="VFY27_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764376.1"
                     /translation="MVDDDLSDTAELELMDTDVMEQLPDAAAETAVTYDGLAGSDAPT
                     DQPKEQGSRPGFDPYNHS"
     gene            complement(3251..3901)
                     /locus_tag="VFY27_02330"
     CDS             complement(3251..3901)
                     /locus_tag="VFY27_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HEX5764377.1"
                     /translation="MLSNRSLPVAAFIAIVMSVAACSSTYYRALESVGIEKRDVLVDR
                     IDDARDSQADAKEQFTSALDNYRALISVEGGDLEKTYDRLNSAYERSERRATEVSDRI
                     RAVEKVAEDLFDEWEEEIDEYSDPALSRRSAQLLDETSKDYDDVITAMRRAEESMSPV
                     LTLFHDQVLFLRHNLNARAIGSLQAELDTIEQATTSLIREMEEAIAEAGRFIDAAG"
     gene            complement(4055..5110)
                     /locus_tag="VFY27_02335"
     CDS             complement(4055..5110)
                     /locus_tag="VFY27_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764378.1"
                     /translation="MHTEKVKQGMRKARKAGLQYIHDLEGGWMRRRRGRGFIYTTARG
                     KRLTGTRTLQRIEALVIPPAWEDVKICPRPNGHLQAVGRDAKGRQQYIYHPDWHAISS
                     ATKFDRLPEIGSLLPRIRRRVRRDLAHKELTRERVVAAVVRLIDRARLRVGNLVYADD
                     NGSHGATTLTPEQVQIDDISISLDFPGKSGKQTEVLLSDEKVAAVIEQCEEINGQFLF
                     CYREDDDSYRSVSSSDVNEYLRSVADEEITAKDFRTWSASVLALKHLRAGLEEADGKV
                     TKRLVNRAIEKTAEEMAHTKAVCRQSYIHPALIAACETGKLPDLLQKYCDPDPDSPAE
                     LTRDERQFLALLPALSR"
BASE COUNT          986 a         1576 c         1508 g         1131 t
ORIGIN      
        1 ccctgtgact tgacgccgcc gtcgcgggcg gtatcgaaaa ctgcgagcca gcgtgttccg
       61 ctcgcgagcg taggcaacgt gaatggcaca ggctcgtgat gggcgttcat caggaccagg
      121 aaggtgtcgt ccagtattcg atcgccccga gcatcgagct cctcgatacc ttcacccggc
      181 aacagcaccc cgagcgtttt cgcgtgagag cttgcccact cttcctcggt catctcgact
      241 gcggcagggt caagccagag aatatcttta acactcgcac ccttgatcgc ctggccctgg
      301 aaaaagcgcc gcctgcgaaa taccgggtgc gccttgcgca gggaaatcag ttgctgcacg
      361 aaggcgagca gtgaccggtc ctcgttaccg aactcccagt tcatccagct aagctcgttg
      421 tcctggcagt agacattgtt gtttcctttc tggcggcggt tcacttcgtc tcctgccagg
      481 agcatcggca caccctgcga aagaaacagc gtggcgagaa aatttcgttg ctggcgcgcc
      541 cggagggtca gcacttcggt gtcgtccgtc tcaccttctg ccccgcaatt ccaactgcgg
      601 ttatgggatt cgccgtcctg gttgttttca aggttcgctt cgttgtgctt ctcgttgtaa
      661 ctcaccaggt cgcgaagagt gaagccgtcg tgggcggtga tgaagtttat gctcgcgacc
      721 ggccgcctgc cgttgcgcgc atagagatcg ctggaacctg tcaggcgata ggcgagatca
      781 ctgatcaagc cgccctcgcc gcgccagtaa tcccggatgt ggtcacggta acgcccgttc
      841 cattcggtcc agcccggcgg aaagttgccc acctggtagc ccccttcgcc gatatcccag
      901 ggctcggcga tcaacttgac ctgcgacagg accgggtcct ggtgaatggt gtccaggaac
      961 gccgtcagcc ggctcatgtc gtgcagttcc ctggcgagcg cggcggccag gtcgaagcgg
     1021 aagccatcca cgtgcatctc cagcacccag taacgcaggc tgtccatgat gagctgcagc
     1081 gtgcgggcat tacccatgtt gagggtgttt ccgcaacccg tatagtcggt gtaataccgg
     1141 ggccgatccg cctccgtgcg gtaataagtc aggttgtcga tgcccctgaa gctgagcgtc
     1201 gggccgaggt gattgccttc cgctgtatgg ttgtaaacga catcgaggat cacctcgatg
     1261 cccgcagagt gcagcgtttt gaccatcgac ttgaattctg tcacagcatc ggtcgagcgg
     1321 tagcgcacgt ccggcgcaaa gaacccgatg gtgttgtagc cccagtagtt ggaaaggcct
     1381 ttgtccacca ggtgcctgtc gctgatggcg tagtggaccg gcatcagctc cacggcggtg
     1441 acgccgagtt cctgcaggta ttcgatgatc gccggtgtcg cgaggccggc gtacgtgccg
     1501 cgcaattcgg gcgcgacgtc gggatgctgc acggtgaaac ccttcacgtg cagctcgtag
     1561 atgacggttt cgtgccaggg ggtccgcggc ggccggtcat ctccccaggt gaacgcggag
     1621 tcgatgaccc ggcatttcgg catatccgcc gagttgtcac gatcgtcgat cgcgagatcg
     1681 gcctgcttgt cgcctacgcg atatgcaaag tgagagtccg accacccgac ccacttttcg
     1741 atgctcttgg catacgggtc cagcaacagt tttgccgggt tgaacctgtg gccctcgcga
     1801 ggcctccagg gaccgtgcac ccggtaaccg tagcgttgcc cgggccgcgc atccgggagg
     1861 tagcaatgcc aaacctggtc tgtctgctcc ggtaatacga cgctttgccc ctgggcttcg
     1921 tcatgctcgg agtaaaggca cagctcgacc ttgtcggcgt gctccgtgaa cagcgcgaag
     1981 ttcacgccct ccccgtccca cgtggcgccg agtggatagg gtttgcccag ccagactctc
     2041 gaaaccttgc ggttcaacga ccctctgttc tctgtcaatc ttaagcaacc tcgggactgg
     2101 actggtggga aatgttcacg caacgcgccg gtacagcggt agtagatgtg tgccggtctg
     2161 cgtcgcgttc gtctgcaact tgcatgccat agcctggcgg cttggagccg aggccgaaac
     2221 gcgcttcagc tggaatgtcg tcgcagaggg gacgctgaag ctgtacgagt cgcacctgac
     2281 agccgcggac ggcctggtaa ttctactggt atgatttttg cagcacgcct gtcacgtcac
     2341 ttaccagaga ccggagactt gcgatgctca aatttctagg cggaactata ggcgttatct
     2401 tcctgattgg cctcattgtt gtgattcttc tcttgatgct gattttctag gcttgcaccc
     2461 tgctcagtgc ttcgctgaat gctaacgttg tagtcagcaa cggcgcttcc ctgcgacacc
     2521 tggccactga cgccacagaa cgttacttgt gctatcggta gtttttaccg agaggctgat
     2581 aacgtctttg cgatgcagaa atgagacggt gcagagcagg atcagcgcag gggaggatca
     2641 cccagcgcgc aaagatcgcg atgcccagaa ttgccagcag gctcagtacg gcgccgatcc
     2701 acatcaaggc caaaaacttc actatcgaca gcggaaatgt ggacagcagc agtgtcgagg
     2761 atattagcgc agtgtatgtt cccatcttcc ctcactgatt cagcgaccgc agccagcggc
     2821 gcgaacaaag ccaggcgtcg cagaatacca agcaaatgtc gtgccacaat ttctggcgct
     2881 cccgtcgccg cacatctcgc cgttttcatg aaccgcagtg tgcaatagcg aaaatgcgcg
     2941 ccagccggaa gaaaagccga cacctgtcac ggaccttgcg cactgcacgc attactgtgc
     3001 cggcgttgaa ctcattcgtg gcgcgacgca ggcaaagcta tggttgacga tgatctgtcc
     3061 gacacggccg aactggagct gatggacacc gatgtaatgg aacagctgcc ggatgctgcc
     3121 gcagaaactg ccgtgacgta cgacggtctt gccggcagtg acgcgccgac tgaccaaccc
     3181 aaagaacaag gaagcagacc cgggttcgac ccttacaacc atagctgagt gggagcactt
     3241 cccacgccgc tcagcctgcc gcgtcgatga aacgtccggc ctcggcgatt gcctcctcca
     3301 tctcacggat aagcgaagta gtggcctgct cgatcgtatc gagttccgcc tgcagtgagc
     3361 cgatggcccg ggcattcagg ttgtgccgca ggaaaagtac ctggtcgtgg aacagtgtga
     3421 ggaccgggct catggattcc tccgcgcggc gcatggcggt tatgacgtcg tcgtaatcct
     3481 tgctcgtctc gtcgagcaac tgtgcgcttc gacgactcaa tgccgggtca gagtactcgt
     3541 cgatctcttc ttcccactcg tcgaagaggt cctctgcgac cttctctacc gcccgaatgc
     3601 gatcggacac ttcagtagcg cgacgctcac tcctctcata ggcggagttc agccggtcgt
     3661 aggtcttctc gaggtcgccg ccttcgacgc tgataagggc gcgatagttg tccagggcgg
     3721 aggtgaattg ttcctttgca tccgcctggg agtcgcgcgc atcgtcgatc ctgtcaacga
     3781 gtacatcgcg cttctcgatg ccaaccgatt ccagcgcgcg atagtaggta gatgagcagg
     3841 ccgctaccga catgacaatg gctatgaacg cagctacagg taaacttcta ttggacagca
     3901 cggtcgatcc tcgtggcctc tgcgcggtaa gaatctcacg ttgcgttacc tgcatgccac
     3961 aggaatttgc agcgtcttcc tccggcgcaa cagggcatta ctacggtgta accgtcaaga
     4021 accactgaac gaggcattaa gcgagactgg cggttcagcg gctcagggcc gggagtagcg
     4081 cgagaaactg tcgctcatcg cgcgtcagct ctgccgggct gtcgggatcc gggtcgcaat
     4141 acttctgtag caagtccggc aacttcccgg tttcgcaagc ggcaatcagc gccgggtgga
     4201 tgtagctctg ccggcatacc gctttagtgt gggccatctc ctccgcggtc ttttctatcg
     4261 cccgatttac gaggcgcttc gtcaccttgc cgtctgcctc ctccaggccg gctctgaggt
     4321 gcttcagcgc cagcacgctc gcagaccacg tgcggaaatc cttcgctgtt atctcctcgt
     4381 ccgcgacgct tcgcaaatat tcgtttacgt ctgacgaact gacagaacga taggagtcgt
     4441 cgtcctcgcg gtaacagaac aggaactgcc cgtttatttc ttcgcactgc tcgatcaccg
     4501 ccgcaacctt ttcgtccgac agcagcactt cggtttgctt gccgctcttt cccgggaaat
     4561 cgagcgatat gcttatgtcg tcgatctgca cctgctccgg cgtcagcgtt gttgcgccgt
     4621 gcgagccatt gtcgtctgcg tacactaaat tgcccacccg gaggcgagcc ctgtcgatca
     4681 aacggacaac tgcggcaacc actctttctc tcgtgagctc cttgtgtgcg agatcccgcc
     4741 tgacccgccg gcggatccgc ggaagcagcg aaccgatctc cgggaggcgg tcgaacttgg
     4801 ttgccgaact gatcgcatgc cagtccggat ggtaaatata ctgctggcgt cctttcgcat
     4861 cccgcccgac tgcctggaga tggccgttcg gccgcgggca gatcttcaca tcctcccagg
     4921 ccggcgggat gacgagggct tcgatgcgtt gcaacgtgcg tgttccggta aggcgcttgc
     4981 cacgcgctgt cgtatagata aagcctcgcc cgcgccgccg ccgcatccag ccaccctcga
     5041 gatcatgaat gtactgcagg ccggccttgc gcgctttacg catgccctgc tttacctttt
     5101 ccgtgtgcat ggttcctgtg cctgggggat ttgccgttac tgccggggaa tcctactaca
     5161 cgcccggagc gtgttatgcc gaaaagatat ttttgtcctc c
//
LOCUS       DASUOM010000116         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_272063,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000116 DASUOM010000000
VERSION     DASUOM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272063"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="sat"
                     /locus_tag="VFY27_02340"
     CDS             complement(<1..971)
                     /gene="sat"
                     /locus_tag="VFY27_02340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5764379.1"
                     /translation="MIKPHGAEALKPLLVSDPGEKAALLEEAEALPSLTLNSAAAANA
                     VMLGGGYFTPLDGFMDLDDALSVSEKMRTTDGLFWPVPILNLTDDVSTIEGASRIALR
                     DPNVAGKPVLAVMNVRKIEHVTPEQIDTMAGQVFGTLDDAHPGVAVFRKLGNYCISGD
                     IQVLNLSYFETEFPGTFKTAVELRDEIRERGWDKVVAFQTRNPMHLAHEELCRMAMER
                     LNADGIVIHMLLGKLKAGDIPAPVREACIRKMVEVYFPDNTVSVSGYGFDMLYAGPRE
                     AVLHAIFRQNMGATHLIVGRDHAGVGSYYGPFDAQAIFEEQVPKGAL"
     gene            complement(995..1666)
                     /gene="cysC"
                     /locus_tag="VFY27_02345"
     CDS             complement(995..1666)
                     /gene="cysC"
                     /locus_tag="VFY27_02345"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX5764380.1"
                     /translation="MAEQKATNVTWHSGDVSREDRYEILRQKGATIWFTGLSGSGKST
                     IAVALERALFGMGKLSYRLDGDNVRLGINKNLGFSETDRKENIRRIGEVAKLFGDAGT
                     IALSSFISPYKGDRDEVRELHNAAGLNFVEVFVDCSLDVAEQRDPKGLYKKARAGQIR
                     NFTGIDDPYEPPENPEIHLKTDEMTLEDEVATVINYLVKNEIIREQYFTRADDKVDSA
                     SAVAV"
     gene            complement(1684..2358)
                     /locus_tag="VFY27_02350"
     CDS             complement(1684..2358)
                     /locus_tag="VFY27_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5764381.1"
                     /translation="MSTEAEAVSKQHADNARYRHQRQEAIKAAASVFAEKGFHGASTR
                     DIAERLGIKQGSLYYYFHSKEEALGEVCLFGIEDYVRRMERIAKSDQPFEAKLLATLT
                     SHLSSYREKNEALKVYNDERLYLPEGKRKVLKQLGSGYRQLLEGVFEEGIRQGVIRNS
                     IDCHFAAQAVIGICNAWGDLIVRDPDLDIFDVTQKSADLLLNGFSERRKTKRETDDNV
                     ALATDH"
BASE COUNT          474 a          784 c          742 g          585 t
ORIGIN      
        1 tccagtgcgc ccttcggcac ctgttcctcg aaaatcgcct gtgcatcgaa cggaccgtaa
       61 tagctgccta cgccggcatg gtcgcgaccg acgatgaggt gcgtcgctcc catgttctgc
      121 ctgaatattg cgtgcagcac cgcctcacga ggtcccgcgt aaagcatgtc gaagccgtag
      181 cccgacaccg aaacggtgtt gtccgggaag taaacctcga ccatcttgcg gatgcaggct
      241 tcacgaaccg gtgccgggat gtccccggcc ttcagcttgc cgagcagcat gtggatcacg
      301 atgccgtctg cattcagccg ctccatcgcc atgcggcaca gctcttcgtg cgcgaggtgc
      361 atgggattac gcgtctggaa cgccaccacc ttgtcccagc ccctttcccg gatctcgtcg
      421 cgaagttcta cggcggtctt gaaagttccg gggaactccg tctcgaagta gctcaggttg
      481 agaacctgga tatcgccgga gatgcagtag ttgccgagtt tgcggaaaac cgcgacgccg
      541 ggatgcgcat catccagcgt gccgaatacc tgcccggcca tcgtatcgat ctgctccggg
      601 gtaacgtgct cgatcttgcg cacgttcatg acggccagca ccggcttgcc cgcaacgttc
      661 gggtcgcgca gcgcgatccg gctggcgcct tcgatggtgc tgacatcgtc ggtcaggttc
      721 aggatcggta ccggccagaa caggccatcg gtcgtgcgca tcttctccga caccgacaat
      781 gcatcgtcga gatccatgaa accgtcgagc ggcgtgaagt aaccgccgcc gagcatcacc
      841 gcattagcgg ccgctgcaga gttaagggtg agcgacggca gcgcttcggc ttcctcgagc
      901 agcgctgctt tttcgcccgg atcggacaca agtaggggtt tcagggcttc tgccccgtga
      961 ggtttgatca cgaaaaaatc tcctggtttt gctgctatac ggctacggct gaagcgctgt
     1021 cgactttgtc atcggcgcgg gtgaagtact gttcgcggat aatctcgttc ttcacgagat
     1081 agttgatgac ggtcgcgact tcatcttcga gcgtcatctc gtcagtcttc aggtggattt
     1141 ccgggttctc cggcggctca tatggatcgt cgatgccggt gaagttcctg atctggcccg
     1201 cgcgagcctt cttgtacagg cccttcggat cccgctgctc cgccacgtcc agcgagcaat
     1261 cgacgaagac ttcgacgaag ttgaggccgg cggcgttgtg cagctcccga acttcgtccc
     1321 ggtcgccttt gtaggggctg atgaagctcg acagtgcgat tgtgccggcg tcgccgaaca
     1381 gcttcgcaac ttctccgatg cgccgaatgt tctccttgcg gtctgtctcg gagaaaccga
     1441 ggttcttgtt gatgcccagc cggacattgt cgccgtcgag tcggtaggac aattttccca
     1501 tgccgaacaa tgctcgctcg agggcgactg caatggtgct cttgccgctg ccggagagcc
     1561 cggtgaacca gatcgtagcg cccttctggc gcaggatctc atagcggtct tcccgcgata
     1621 catcaccgct gtgccaggtg acgtttgtag ctttttgttc ggccataaat ccctccgtag
     1681 gtttcagtga tccgtggcga gagcgacgtt gtcgtcagtc tcgcgtttgg tcttgcgtcg
     1741 ttccgagaac ccgttcagca gcaaatcggc gctcttctgg gtcacgtcga aaatgtcgag
     1801 gtcggggtcg cggacgatga ggtcgcccca cgcgttgcag atgccgatta cggcctgcgc
     1861 ggcgaagtgg cagtcgatgg agtttctgat cactccctgc cgaattccct cctcaaaaac
     1921 tccttcgagc aactggcgat atccgcttcc gagttgcttc agcaccttgc gcttcccttc
     1981 cggcaggtac aggcgctcgt cgttgtaaac cttgagcgcc tcgttcttct cccggtaact
     2041 cgagaggtgc gaggtcagcg tcgccagcag cttggcctcg aatggctgat cgctcttcgc
     2101 gatgcgctcc atacgacgca cgtaatcttc tatgccgaac aggcagacct ccccgagagc
     2161 ttcttccttg gaatggaagt agtaataaag gctgccctgc ttgatcccca ggcgctccgc
     2221 gatgtccctg gtgctggcgc cgtgaaatcc cttctcggca aatacggaag cggcggcttt
     2281 gatcgcttcc tggcgctgat gacggtaacg cgcgttatcc gcgtgctgct tcgaaaccgc
     2341 ttccgcctcg gtgctcatga cgcggcattc tatagcgcta tagattcctg tcaacgaata
     2401 tctctgttgg ctttattatc aaacagttat gtgtttacat aaccaatcaa gctattgacg
     2461 gcagccgcta acacgcgaac ttcagcgggg gagcagcgag atcagcttca ggccgaacca
     2521 gacggcggtg acgccaagga cgacacttgc accggcgtat agcgcggcca cccaaaactc
     2581 acctc
//
LOCUS       DASUOM010000117         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1193392,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000117 DASUOM010000000
VERSION     DASUOM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193392"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="glnL"
                     /locus_tag="VFY27_02355"
     CDS             <1..1188
                     /gene="glnL"
                     /locus_tag="VFY27_02355"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX5764382.1"
                     /translation="PADDSQRTDPLLRAADDDRKPARRLTWQDGSRNPDTGSHDDAAE
                     RSLAFDIVDNLYTSVFVLDRELSIVVINSAGESLLGISRHRSRGRSLLQLLGNDRKFS
                     DVLDRVLSTGQTYADELKLGPVEVGGDDRVFDCRASAIRSCEEDLLLVELSDVTRRSR
                     ISRENALIVQHGAGRQMIRQLAHEIKNPLGGLRGAAQLLARQLPEEELKEYTSVIISE
                     ADRLASLVDTLLGPGGPPDKRPVNIHELLEYVLKLIQPQAGFALRIRREYDPGLPFID
                     LDRDQMVQAFLNLLQNSATALAGHGEIVVRTRATTNFTIGDVRHRAIASIEIEDDGPG
                     VPPELSDSVFYPLVTGRPDGTGLGLPVAQDLVSRHGGLIEFESRPGKTVFFVRLPLSS
                     TRE"
     gene            1191..2597
                     /gene="ntrC"
                     /locus_tag="VFY27_02360"
     CDS             1191..2597
                     /gene="ntrC"
                     /locus_tag="VFY27_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX5764383.1"
                     /translation="MADRAIDVWIVDDDQSVRWVMEKALKQAGMQPRSFERAEHLLEA
                     IERTQPDVLITDVRMPGMSGLALLTQLKRDHPGLPVIVMTAHSDLDNAVAAYHGGAFE
                     YLPKPFDLDETMALVQKAARQNGKAEVRVSNDGAAAMPALIGKAPAMQEVFRSIGRLA
                     RSSMTVLITGESGTGKELVARALHQHSPRSLKPFVALNTSAIASELLESELFGHERGS
                     FTGADARRIGRFEQADGGTLFLDEIGDMSPGLQTRLLRVLAESEFFRVGGQTSITVDV
                     RVIAATSQDLALAVREGRFRDDLFHRLNVMRINTPPLRNRRQDIAPLLQHYLGEAARE
                     LDAAPKTLSAEAHAILEAYDWPGNVRQLVNAARRLTVTAPGRVITAADIGEDLGGSAS
                     RKGAMQEWSLGLENWAEQELASQSGGPLLQSALPEVEKTLIRVALKKAGGHRQEAAKL
                     LGCGRNTLTRKIRSLDID"
     gene            complement(2634..2771)
                     /locus_tag="VFY27_02365"
     CDS             complement(2634..2771)
                     /locus_tag="VFY27_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764384.1"
                     /translation="MGVAEPSPATCHLRRLAVCIESYTTVVVELDDRTIQALRGLAGS
                     E"
     gene            complement(2762..3109)
                     /locus_tag="VFY27_02370"
     CDS             complement(2762..3109)
                     /locus_tag="VFY27_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5764385.1"
                     /translation="MKYLCLIYSDEKKTNAIPAGEMQAVVDECEPYTAALRVKGQLIA
                     AERLKPTGTATTLKFDGGKLSITDGPFAETKEQLGGFLLIDARDLDEALSIAAEMPPA
                     RLGGSVEVRPVWE"
     gene            complement(3195..>3701)
                     /locus_tag="VFY27_02375"
     CDS             complement(3195..>3701)
                     /locus_tag="VFY27_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5764386.1"
                     /translation="GNYPEMSAQVVAACRSATSKPIIAKLSPNQADIAENARRCIEAG
                     ADGLAVINTIAGMSINAEKRAPVLGNVRGGLSGPAIKPIALLKVRQVHEIAGPLGVPV
                     IGQGGLTTATDAVEFLLAGATAVGVGTALFYDPLVCRAINNGILDYMVRHRFPSVSAI
                     TGALAAPI"
BASE COUNT          703 a         1176 c         1168 g          654 t
ORIGIN      
        1 ccagctgatg attcgcagcg aaccgacccg cttctacgtg cagcagacga cgatcgtaaa
       61 cccgcgcgga ggttgacctg gcaagacggg tcccggaacc ctgacacggg ttctcacgat
      121 gatgccgccg agcgctcgct cgcgttcgac atcgtcgaca atctctacac cagcgtattc
      181 gtcctcgatc gtgaactctc gatcgtcgtc atcaactccg ccggtgaaag cctgctcggc
      241 atcagccggc atcgttcgcg cggtcgttca ctgttgcagc tgctcggcaa cgacaggaaa
      301 ttcagcgatg tcctcgaccg cgtgctatcg acaggccaga catacgcgga cgaactcaag
      361 ctcgggcccg ttgaagtggg cggcgatgac cgcgtcttcg actgccgggc ttccgcgata
      421 cgctcctgcg aagaggactt gctgctggtg gaactgagcg acgtcacgag gcgatcgcga
      481 atctcccggg agaacgcact catcgtgcag catggagcag gccgccagat gatccggcag
      541 ctcgcccacg agatcaaaaa cccgctgggc gggctgcggg gagcggccca gctcctggcg
      601 cggcagctgc cggaggagga gctgaaggaa tacaccagcg tgatcatcag cgaagccgac
      661 aggcttgcat cgcttgtcga caccctgctc ggccccggcg gcccgccgga caagaggccg
      721 gtcaacatcc acgaactgct ggagtacgtg ctgaaactga tccagccgca ggccggtttc
      781 gcgctccgca ttcgccgcga atacgatccc ggcttgccgt tcatcgatct cgaccgcgac
      841 cagatggtgc aggcgtttct gaacctgctg caaaactcgg cgactgcgct cgcgggacac
      901 ggcgagatcg tggtgcggac ccgtgccacg accaacttca cgatcggcga cgtcaggcac
      961 cgcgctatcg cgagtataga aatcgaggac gacggcccgg gtgtcccgcc ggaactttca
     1021 gactcggtct tttatcctct cgttacgggc aggcccgacg gtaccggcct gggcctgccg
     1081 gtcgcgcagg acctcgtcag ccggcacgga gggttgatcg agttcgagag ccgccccggc
     1141 aagacggtct ttttcgtccg cctgccgctt tcaagtacga gggagtgaac atggctgacc
     1201 gggcgatcga tgtgtggatc gtcgatgacg atcagtcggt tcgctgggtg atggaaaagg
     1261 cactgaagca ggccgggatg cagccgcgga gcttcgagcg tgcggaacac ctgctcgagg
     1321 ccatcgagcg cacacagccg gacgtgctga tcaccgacgt gcgcatgccc ggcatgagcg
     1381 gcctcgcgct gctcacgcaa ctcaagcgcg atcatcccgg cctgccggtg atcgtgatga
     1441 ccgcgcactc ggacctggac aacgccgttg ccgcctacca cggcggggcc ttcgaatacc
     1501 tgccgaaacc cttcgatctc gatgaaacga tggcgctggt gcaaaaggca gcgcggcaga
     1561 acggaaaggc ggaagtgcgc gtaagcaacg acggtgcggc tgccatgccg gcattaatcg
     1621 gcaaggcgcc ggcgatgcag gaagtgttcc gctccattgg cagactggcg cgttccagca
     1681 tgacggtact gatcaccggc gagtcgggaa cgggtaagga gctggtggcg cgtgcgctgc
     1741 accagcacag cccgcgcagc ctgaagccat tcgttgcctt gaacacgtcg gcgatcgcct
     1801 ccgagttgct ggagtccgaa ctgttcggtc atgaacgcgg ctcctttacc ggcgctgatg
     1861 cgcgccgcat cgggcgtttc gagcaggcgg acggaggtac gctgtttctc gacgagatcg
     1921 gcgacatgtc tcccgggctg cagacacgcc tgttgcgcgt actcgcagaa agcgagttct
     1981 tccgcgttgg cggacagacg tcgatcaccg tggatgtcag ggtgatcgca gcgacgagcc
     2041 aggatctcgc gctagcggtg cgcgaaggcc ggtttcgcga cgaccttttc catcgtttga
     2101 acgtcatgcg catcaacacg ccgccgctta ggaatcgccg ccaggatata gcgcccctgc
     2161 tccagcacta cctcggtgaa gctgcccgtg aactggatgc cgctccgaaa accctgagcg
     2221 cggaagctca tgcgattctc gaggcctacg actggccggg caacgtacga caactggtca
     2281 acgcggcacg gcgcctcacc gtcaccgcac cgggacgagt catcaccgcg gccgacatcg
     2341 gcgaagacct gggaggcagc gcgtcacgca agggcgcgat gcaggaatgg tcgctcgggc
     2401 ttgagaactg ggcggagcag gaactggcat cacagagcgg cgggccgttg ctgcagtcgg
     2461 ctttgccgga agtcgaaaag acgctgatca gggtcgcgtt gaaaaaagcg ggcggacacc
     2521 ggcaggaagc agcaaagctc ctcggctgcg gcaggaatac cctcacccgc aaaatacgat
     2581 ccctcgacat cgactaagag gtcgcaggtg acaagcctgt gcttagcggt tccctactcg
     2641 ctacccgcga gcccgcggag cgcctggatc gtccggtcgt ccagttccac taccacggtc
     2701 gtgtagctct ctatgcagac ggcaagccga cgcaggtggc aggtggcagg tgaaggttcc
     2761 gctactccca taccgggcgc acctcgacgg agcctcccag ccgagcgggc ggcatctctg
     2821 cggcgatact taacgcctcg tccaggtccc tggcatcgat cagcaggaaa cctcccagtt
     2881 gctccttcgt ctcggcgaac ggcccatccg tgatagacag cttgccgcca tcgaacttca
     2941 gggtggttgc ggtgccggtc ggcttcaggc gctctgccgc gatcagctgg cccttgaccc
     3001 tgagcgcggc ggtatacggc tcgcactcgt cgaccacggc ctgcatctcc ccagccggaa
     3061 tcgcgtttgt tttcttttcg tcgctgtata tgaggcaaag gtacttcatc gattactccc
     3121 gctatcaact gctatgtctc gtagtcgttc cggacgaacg agattagaca gccggattcg
     3181 tgaaggtggc gcagctatat gggcgcggca agcgccccgg ttatcgcaga aacagaggga
     3241 aaacggtgcc gcaccatgta atcgaggatt ccattgttga tggccctgca caccagcggg
     3301 tcgtagaaca aggccgtgcc gacacccacc gcggtggcgc cggcaagcag gaattccacg
     3361 gcgtctgtcg ccgtggtcag cccaccctgg ccgatgacag gaacgccgag cggtcccgcg
     3421 atttcatgca cctgccgcac tttcagcagg gcaatcggtt tgatggccgg tcccgacaga
     3481 ccgccccgaa cgttgcccaa caccggcgcc cgcttctccg cgtttatcga catcccggcg
     3541 atggtgttga tcactgcgag cccgtcggcg cctgcttcga tgcagcgcct cgcgttctct
     3601 gcgatgtctg cctggttcgg cgacagcttc gcgatgatgg gtttgctggt ggcggatcgg
     3661 caggcggcga caacctgcgc cgacatctcg gggtaattgc c
//
LOCUS       DASUOM010000118         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1358957,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000118 DASUOM010000000
VERSION     DASUOM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358957"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFY27_02380"
     CDS             <1..567
                     /locus_tag="VFY27_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX5764387.1"
                     /translation="YTVRKGAIIAYISADGRYLLQGDLIDLDEEVNLSEQDRNEARVE
                     MLAAIPEENMIVFSPEVPRFSVTVFTDIDCTYCRRFHSQIDEYLAQGIEVRYLLYPRN
                     GPAAESWAKAEKVWCADDRNEALTLAKLDREFETRSCDAAIVHKHYATGQDVGLRGTP
                     AIVLQDGTLVSGYLPPLELSQAVASVKP"
     gene            complement(590..1108)
                     /locus_tag="VFY27_02385"
     CDS             complement(590..1108)
                     /locus_tag="VFY27_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5764388.1"
                     /translation="MNCGKTVSRKVPAGDTRVRLICEGCSAIHYQNPKIVVGCVPEQD
                     GKILLCRRAIEPRRGFWTVPAGFMELGETVAEGAARETREEACAKVEIGRLFASVDVI
                     EAGQVHLFFAARLLGGYGVGEETLEAELFPKDDIPWTEIAFLSGRFALEKYLEDDGRN
                     NGVHLHRVRHGE"
     gene            complement(1202..1288)
                     /locus_tag="VFY27_02390"
     tRNA            complement(1202..1288)
                     /locus_tag="VFY27_02390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1252..1254),aa:Leu,seq:caa)
     gene            1418..2026
                     /locus_tag="VFY27_02395"
     CDS             1418..2026
                     /locus_tag="VFY27_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5764389.1"
                     /translation="MSKFTDIQIDPGIVQRAARGDVHAHEILYRAYGSPVYSICLRFT
                     RVPAHAEDLTQETFVEVIRSIAGFRGDAPVGAWIRRIAVSKALMFLRSAWHSRGQSLD
                     DDWEESPEGRHAGTQSEHPEQAIDLDAALGALPPVSRAVIWLHDVEGFTHKEIAALMG
                     KTESFSKSQLSRAYQRLRPMLEAETAATAGKAEMATRALGSC"
     gene            2083..2673
                     /locus_tag="VFY27_02400"
     CDS             2083..2673
                     /locus_tag="VFY27_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764390.1"
                     /translation="MPDTETEQDETMISGLGVRERELLHARLRQLPETMPPRAVWQRI
                     EAQARAEGLLKTSLPRRLKLVAGAGLAATVVLAVVGVQDARNGAESETFPTVPSYSEQ
                     NNVVALNTLNALMVQSQELEEDLRALPSQPQLVRAGTAATIAGLEDQIAAIDYRLNEP
                     SIRMSHEQMEVYWRERVRLMNLLVQLRAAQAQRNGF"
BASE COUNT          550 a          841 c          906 g          488 t
ORIGIN      
        1 tacacggttc gcaagggcgc gatcattgcg tacatctcgg cggacgggcg ttacctgctg
       61 cagggcgacc tcatcgacct cgacgaggag gtcaacctga gcgagcagga ccggaacgag
      121 gctcgtgtcg agatgcttgc cgccattccc gaagagaaca tgatcgtgtt ctcgccggag
      181 gtaccgcgct tttcggtgac ggtctttacc gatatcgact gcacgtattg ccggcgcttc
      241 catagccaga tcgatgagta cctggcacag ggaatcgagg ttcgatacct gctttatccg
      301 cggaacgggc ccgctgcgga gtcctgggcc aaggcggaga aagtgtggtg cgccgacgat
      361 cgcaatgagg cgctgacgct cgcgaagctg gatcgtgaat ttgaaacccg cagctgcgat
      421 gcggcgatcg tgcacaagca ctacgcgacg ggccaggatg tcggattgcg cggtactccc
      481 gccatcgtgc tgcaggacgg aacgttggtg agcggctacc tgccgccgct cgagctttcg
      541 caggcggtcg cgtcagtcaa gccctgaacc ggcaagaacg gcacggccgc tattctccgt
      601 gccgtacacg gtgcaggtgg accccgttgt tgcggccgtc gtcttcgagg tatttctcca
      661 gcgcgaagcg gccgctcagg aaagcgatct ccgtccaggg aatgtcgtct ttggggaata
      721 gctcggcctc cagcgtttcc tcgccgactc cataaccgcc gagcagcctc gctgcgaaga
      781 acaggtgcac ctgtcctgcc tcgatgacgt cgaccgaggc gaacaaacga ccgatctcca
      841 ccttcgcaca ggcctcttcg cgcgtctcgc gcgccgcgcc ttcggccact gtttcgccga
      901 gttccatgaa accggccggc acggtccaga aaccgcggcg cggctctatg gcgcgccggc
      961 aaagcaggat ttttccgtcc tgctcaggta cacagccgac cacgatcttc gggttctggt
     1021 aatggatcgc gctgcaaccc tcgcaaatca gacgtacacg agtatcgccc gcgggtacct
     1081 tgcggctgac ggtcttgccg cagttcatgc agaatttcat tatggagtgt caactggcct
     1141 ttgaagtccg ctcgggacaa gcgttcgttg agatgaattg agcttgtgca cctggtctgg
     1201 atggtgccgg gagcgagaat cgaactcgca ctctgtttcc agaaccggat tttgagtccg
     1261 gcgcgtctac cagttccgcc atcccggccg aaaggcgcca agtatagagc ctgccggaga
     1321 tattgcacca ccggcggggg cagcgcggca gggtgctttc ggccatcacg acagcctgcg
     1381 acgctttcgc gaaagggcgc atctacactg gcatggtatg agcaagttca ccgacatcca
     1441 gatcgacccc gggatcgtgc agcgcgcggc gcgcggcgac gtgcacgccc acgaaattct
     1501 ctaccgggcg tatgggtcgc cggtgtactc gatctgcctg cggttcaccc gcgtacctgc
     1561 acacgccgag gacctgaccc aggagacgtt cgtggaggtc atacgcagca ttgccggctt
     1621 tcgcggtgac gctccggtgg gcgcctggat acggcgcatc gctgtcagca aggcactgat
     1681 gttcctgcgc tccgcctggc acagccgcgg ccagagcctg gacgacgact gggaggagag
     1741 ccccgagggg cgtcacgccg ggacgcagtc ggaacacccg gaacaggcga tcgatctcga
     1801 tgcggcgctg ggcgcgttgc ctccggtcag ccgggccgtc atctggctgc atgacgtcga
     1861 gggatttacg cacaaggaaa tagcggccct catgggcaag accgaaagtt tttcgaagtc
     1921 gcagctctca cgggcatacc agcggctgcg gccgatgctc gaagcggaaa cggcggccac
     1981 cgcaggtaag gcggaaatgg cgacgcgggc actgggaagc tgctgagaga aggtgattcg
     2041 ccagccgctg gaagcaggat tttctgaggt agaggcaaga acatgccgga cactgaaacg
     2101 gaacaggatg aaacgatgat cagcggactt ggcgtacggg aacgcgagct cctgcatgcg
     2161 aggcttcggc agttgccgga gacgatgccg ccgcgcgccg tatggcagcg catcgaagcg
     2221 caagctcgcg cggagggcct tttgaagaca agcctgcccc ggcgcctgaa actggtggcg
     2281 ggcgcgggac ttgcggccac agtggtgctc gcggtcgtcg gggtgcagga cgcgcgcaat
     2341 ggcgctgaaa gcgaaacctt ccccaccgtg ccgtcatact cggagcagaa caacgtggtt
     2401 gcgctcaaca cgctgaacgc cctgatggtc cagtcgcagg agctggaaga ggatctgcgg
     2461 gcactgccca gccagccgca gctcgtgcgt gccggaacgg ccgccacgat tgcgggcctg
     2521 gaagaccaga tcgccgccat tgactaccgg ctcaacgaac catcgatccg catgagccac
     2581 gagcagatgg aggtgtattg gcgggagcgg gtgcgattga tgaacctgct cgtgcagctg
     2641 cgcgccgcac aggcacaacg gaacggtttc tagcgccgaa atcgcaagaa gctcccgcgt
     2701 tcgggcatat cgatgatcaa ggtcaaatca gaagcgcagg acccgcggtc gctgcgcaat
     2761 ttcaggagaa tggacatgtc aggct
//
LOCUS       DASUOM010000119         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_154463,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000119 DASUOM010000000
VERSION     DASUOM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154463"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..133
                     /gene="rrf"
                     /locus_tag="VFY27_02405"
     rRNA            18..133
                     /gene="rrf"
                     /locus_tag="VFY27_02405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            226..1116
                     /locus_tag="VFY27_02410"
     CDS             226..1116
                     /locus_tag="VFY27_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764391.1"
                     /translation="MAKRFVKKYRTKRAFKWLKRRLVPAGVRAARVHRKWRKKDLEDW
                     PPAGTDPAAGLGQYEYSYLSQNGEDGITRFLFSEIGFGSRCLVEFGFGAMQCNSLRLL
                     LHEGFTGLLMDGSEENCDFFNHAARKRGLQGVEARCTFLTRDNLNQTIREHGITGEID
                     FLSLDVDGNDYWFWESLDCVSPRVVCIEYNAGIGPEFSWSVPYDPQFERYAKHPSGFF
                     HGASLKALESLGQRKGWRLVGCDSTGTNAFFLRADVDAPALPTLTAAEAFRPHQNWLG
                     RGFSEAEQLEIMKSMPYEDV"
     gene            1271..>2643
                     /locus_tag="VFY27_02415"
     CDS             1271..>2643
                     /locus_tag="VFY27_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764392.1"
                     /translation="MAFGQESARRETLDEIVVTATRMQSSVRDVPRSVSLVGQERIQN
                     ATQQLALDEALAGVPGLYMQNRYNFSQDLRISLRGFGARSGFGIRGVRVYVDGIPETL
                     PDGQAGVDSIDLGSAKSIEVLRGPASSLYGNASGGVIAIETELGDADPYVEAGLAGGE
                     LGFQKYQLKTGGSAGRLDYLLNASTQEFDGYRDHSYSEGTLVNGKFGYNITDDDRFTV
                     AANHTDQPESYDPGGVNLEELAAGRRNARDVNVLFNAGEGLSQQRIGFVYERDRPHGD
                     LLLRNYYVWRDFSNELPFVDGGAVELDRFFYGVGAQYTPRAMLPESLELTAGFDLDRQ
                     NDDRLRFDNNEGVRGDLTFDQEEKVDSAGIYVQGQYALTEDFRFSAGIRYDELTFDIT
                     DRFLADGDDSGEIDFDQVSPSAGLNYSFGEYVLFGSFSRSFETPTTTELANPDSSGGF
                     NQSLEPQ"
BASE COUNT          521 a          782 c          811 g          529 t
ORIGIN      
        1 cggaacctag ccggttgcct ggcggcaata gcgagcggga accacccgat cccattccga
       61 actcggaagt gaaaacgctc agcgccgatg gtagtgtggg gtctccccat gtgagagtag
      121 gacatcgcca ggctcttaac acgaaaggcc gcagcccgaa agggttgcgg cttttttttt
      181 cgcttcgggt aagatggcgg gccgcccagg ccgggaggtt tccgcatggc aaagcgcttc
      241 gtcaagaaat accgcaccaa gagagccttc aaatggctca agcggcgcct ggttcctgcc
      301 ggtgtgcgtg ccgccagggt acataggaag tggcgcaaga aggatcttga ggattggccg
      361 cccgccggca ccgatcccgc ggccgggctt gggcagtatg aatacagcta tctctcgcag
      421 aatggcgagg acggcatcac ccgcttcctg ttttcagaaa tcggttttgg atcaaggtgc
      481 ctggtcgagt tcggctttgg tgcgatgcag tgcaacagcc tccgactgct cctgcacgag
      541 ggcttcactg gcctgttgat ggacggctcc gaagagaact gcgacttctt caatcacgcg
      601 gcgcgcaaga ggggcctgca gggagtggag gcgcgctgta cttttctcac gcgcgacaac
      661 ctgaaccaga caatccgcga gcacggcatt accggagaga tagatttcct gtcgctcgac
      721 gtcgacggta atgactactg gttctgggag tcactggact gcgtctcgcc gcgggtcgtc
      781 tgcatcgagt acaacgcggg catcggtccc gaattctcct ggtcggtacc ctacgacccg
      841 cagttcgagc gttacgcgaa gcaccccagc ggctttttcc acggtgcatc actgaaggcc
      901 ctggagtcac tcgggcagcg caagggctgg cggctcgttg ggtgcgactc gaccggaacc
      961 aacgcctttt tccttcgagc cgatgtggac gcgcctgcgt tgcccacctt gactgcggca
     1021 gaagccttca ggcctcacca gaactggctt ggcaggggct tttccgaggc agaacagctc
     1081 gaaatcatga aatccatgcc gtatgaagat gtatagtgct aaagcatctg gaatcagtct
     1141 agccggcgcc aaagccacga ttatcagcag gaatcaggta cccatggaca atgagatatc
     1201 tcgccacaac ggcttccggc atcttgccgt ttttcttgcg tgtttctccg ctccctcttt
     1261 ttttgctccc gtggctttcg gccaggagtc tgccaggcgc gagacactgg atgaaatcgt
     1321 cgtcacggca acccgtatgc aatcctcggt gcgcgacgtg ccgcgctcgg tctcgctggt
     1381 cgggcaggag cgaatccaga acgcgacgca acagcttgcg ctggacgagg cgctggccgg
     1441 agtccccggc ctgtacatgc agaaccgcta caacttctcg caagacctgc gtatttcgct
     1501 gcgcggcttc ggcgcccgtt ccggcttcgg cattcggggc gtacgagttt acgtcgatgg
     1561 gatcccggag accctgcccg acgggcaggc gggagtcgac agcatcgacc tgggttctgc
     1621 gaaaagtatc gaggtgttgc gcgggccggc atcttcgctc tacggtaacg cttcgggagg
     1681 agtgatcgcg atcgagacag aactcggcga tgccgaccct tacgttgaag ccggactggc
     1741 gggtggcgag ctcgggttcc agaagtacca gctcaagaca ggcggctcgg ccggccggct
     1801 cgactatctg ttgaacgcgt caacgcagga gttcgacggc tatcgcgacc actcctactc
     1861 ggaaggcacg ctggtgaacg gcaagttcgg ttacaacatc accgatgacg atcgtttcac
     1921 ggtcgccgcc aaccataccg accagccgga gtcgtatgat cctggcggcg tcaacctgga
     1981 agaactggcg gcgggccgcc gcaacgcccg cgatgtgaac gtgctgttca acgccggtga
     2041 aggtctttcc cagcagcgta tcggcttcgt ttacgagcgc gatcggccac atggtgattt
     2101 gctgttgcgc aactattacg tctggcgcga tttcagcaac gaactgccgt tcgttgatgg
     2161 cggcgcagtc gagctggatc gctttttcta tggtgtcggc gcgcaataca cgccacgcgc
     2221 catgcttccg gagagcctgg aactgacggc aggcttcgac ctggatcgcc agaacgacga
     2281 ccgcctccgc ttcgataaca acgagggcgt gcgcggagat ctgacgttcg accaggagga
     2341 gaaagtcgac agcgccggca tctacgtgca ggggcagtac gcgcttaccg aagacttcag
     2401 gttttctgcc ggtattcgct atgacgagct gacgttcgat atcaccgatc gcttccttgc
     2461 agacggcgac gactcgggcg agatcgattt cgaccaggtc agtccctcgg ccggcctgaa
     2521 ctacagcttc ggcgaatacg tgctgttcgg ttcgttcagc aggtcgttcg aaacgccgac
     2581 gaccacggaa cttgcgaacc ctgacagcag cggcggattc aaccagtcgc tcgagccgca
     2641 aat
//
LOCUS       DASUOM010000120         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_877434,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000120 DASUOM010000000
VERSION     DASUOM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877434"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /gene="lon"
                     /locus_tag="VFY27_02420"
     CDS             complement(<1..2207)
                     /gene="lon"
                     /locus_tag="VFY27_02420"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5764393.1"
                     /translation="MDSGKGTPVLPLRDVVVYPHMVIPLFVGRDKSIVALDRAMATGK
                     KILLIAQQKADLDDPAAEDLYDVGTLATILQLLKLPDGTVKVLVEGVERARVDELSLE
                     DHFSAEITILNEEEEHDEREIDVLVRSIIAQFEQYVKLNKKVPPEILTSLSGIDQPGR
                     LADTVAAHMALKLAEKQRILEIQDVKVRLEQILGIIEGEIDVLQIEKRIRGRVKQQME
                     KSQREYYLNEQMKAIQKELGEMEDAPNELADLEAKIENAGMPKEAKDKATSELNKLKL
                     MSPMSAEATVVRNYIDWLVKAPWKKRSKVIRDLARAEQVLEEDHYGLEKVKERILEYL
                     AVQQRVKRLKGPILCLVGPPGVGKTSLGQSIARATNRNFVRMSLGGVRDEAEIRGHRR
                     TYIGSMPGKIIQNLAKTKVRNPLFLLDEVDKMAMDFRGDPSSALLEVLDPEQNSSFND
                     HYLEVDFDLSDVMFVCTANSLNIPAPLLDRMEVIRIPGYTEDEKLNIALRYLIPKQLK
                     ENGLEESDLTITKAAVLDIIQHYTREAGVRNLEREISKICRKVVKSLLLKPRTSALRI
                     TPRTMEKYLGVRRFRYGKAEERDQVGQVTGLAWTEVGGELLTIEAAVVPGKGKLTHTG
                     QLGDVMTESIQAAMTVVRSRAKVLGVDEDFHQKFDVHVHVPEGATPKDGPSAGVGMCT
                     ALVSALTNVPVKSEVAMTGEITLRGEVLPIGGLKEKLLAAHRGGITTVLIPQD"
     gene            complement(2427..>3372)
                     /gene="clpX"
                     /locus_tag="VFY27_02425"
     CDS             complement(2427..>3372)
                     /gene="clpX"
                     /locus_tag="VFY27_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017701334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5764394.1"
                     /translation="PTGCGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQ
                     KLLQKCDYDVEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIAS
                     VPPQGGRKHPQQEFLQVDTGNILFICGGAFAGLEKIILNRSSKSGIGFVAEVKSGDME
                     KSIGELLHDVEPEDLIRFGLIPEFVGRLPVVATLEELDEDALVSILLEPKNALTKQYR
                     KLFDMEGVDLEFRDDALRAVAKRAMKRKTGARGLRTILESVLLDTMYDLPSSTNAKKV
                     VLDEAVVTGETKPYVIYESEEPPTVNLEQQRRPTGSDR"
BASE COUNT          579 a         1040 c          954 g          799 t
ORIGIN      
        1 ttatcctggg gaatgaggac cgtcgtgata ccgccgcgat gtgccgcaag aagcttctct
       61 ttaagcccgc cgatcggtag cacttcgcca cgcagtgtaa tctcgccggt catcgcgact
      121 tcgctcttca ccgggacgtt cgtcagcgcc gacaccagcg cagtacacat gcccactccc
      181 gcgctgggtc cgtccttggg cgtcgcgcct tccggaacgt ggacatgcac gtcgaacttc
      241 tggtgaaaat cttcatcgac gccgagaact ttcgcccggc tcctgacaac cgtcatcgca
      301 gcctggatcg actccgtcat gacgtcaccg agctggccgg tgtgggtcag cttgccctta
      361 cccggcacaa ccgccgcctc gatagtcagc aactcgccgc cgacttccgt ccaggcaaga
      421 ccggtaacct gcccgacctg gtctctctcc tccgccttgc cgtaacggaa gcgccgcacg
      481 ccgaggtact tttccatggt tcgcggcgtg atgcgcagtg ccgacgtgcg tggcttcagc
      541 aacaacgact tcacgacctt gcgacagatt ttcgagattt cgcgctcgag atttcgcaca
      601 ccggcctcgc gagtgtagtg ctggatgatg tccagcacgg ccgctttggt tatcgtcagg
      661 tcgctttctt ccagcccgtt ctctttcagc tgcttcggaa tcagatagcg aagcgcaatg
      721 ttgagctttt cgtcttccgt gtaaccgggg atgcggatga cttccatgcg atcgagcagc
      781 ggcgccggaa tgttgagact gttcgccgta cacacgaaca tcacgtccga aagatcgaag
      841 tccacttcca ggtagtgatc gttgaacgac gagttttgtt ccgggtccag cacctcgagc
      901 agcgccgacg agggatcgcc tcggaagtcc atggccatct tgtccacctc gtccagaagg
      961 aacagcgggt tcctgacctt cgtctttgcc aggttctgga tgatcttgcc gggcatggaa
     1021 ccgatgtacg tgcgccggtg cccgcggatc tccgcttcgt cgcgtacccc gccgagcgac
     1081 atccgcacga aattgcggtt cgtcgcgcgg gcgatgctct ggcccagcga ggtcttgccg
     1141 acgcccggcg gccctaccag gcagagaatc ggtcctttca accgcttgac ccgctgctgc
     1201 accgcgaggt attcgagaat gcgttccttg accttctcga gcccgtagtg atcctcttcc
     1261 agtacctgct cggcgcgtgc caggtcccgt atgaccttgc tgcgtttttt ccatggtgct
     1321 ttgacgagcc agtcgatgta attacggacg acagttgctt ccgccgacat cggtgacatc
     1381 agcttcagct tgttgagttc ggaggttgcc ttgtccttgg cctccttggg catgcctgca
     1441 ttctcgatct tggcctcgag atcggcgagt tcgttgggcg cgtcttccat ctcacccagt
     1501 tctttctgga tggccttcat ctgctcgttc aggtagtact cgcgctggct cttctccatc
     1561 tgctgcttca cacggccgcg aatgcgcttc tcgatctgca gcacgtcgat ctcgccttcg
     1621 atgatgccga ggatctgctc caggcgaact ttcacgtcct ggatttcgag aatcctctgt
     1681 ttctcggcga gcttgagcgc catgtgcgcg gctacagtgt ccgccagtcg cccgggctgg
     1741 tcgatgccgg acagcgacgt caggatttcg ggcggcactt tcttgttcag cttcacgtac
     1801 tgctcgaact gcgcgatgat ggagcgcacc agcacgtcga tttcgcgctc gtcgtgctct
     1861 tcttcctcgt taaggatggt tatctctgcg gagaaatgat cctcgagcga cagctcgtcg
     1921 acgcgcgcgc gctctacacc ttcaacgagc accttcacgg taccgtcggg cagcttcagc
     1981 agttgcaata tcgttgcgag cgtgccgacg tcgtagagat cttccgcggc ggggtcatcg
     2041 agatccgctt tttgctgcgc gattagcagg atcttcttgc ccgtggccat cgcgcggtcg
     2101 agagccacga tggatttatc gcggccgacg aacagcggga tcaccatgtg cggataaaca
     2161 accacatcgc gaagcggcag cacgggtgtt cccttgccgc tgtccacggc atcgctttcg
     2221 tctttaagtt ctaattcggt ggtagtggtt tcgtcttcag acactgaatt accccaggta
     2281 gtgaatgtgt gtgcaccgtc cgttgcggca gaaatgcggc cgtttcagtc gatatcaacg
     2341 gcgcaactac gaatgtagga gccccccatt cgggcaagcg ttcacgaact cgcccgcatg
     2401 gcgggctcct gcgcgcgccg gcgggtttat cggtccgaac cggtgggtct tcgttgctgt
     2461 tcgaggttga cggtcggcgg ttcttccgac tcataaatga cgtatggttt ggtctcaccg
     2521 gtgacgactg cttcatcgag aaccaccttc ttcgcgttgg tgctcgacgg caaatcgtac
     2581 atggtgtcga ggagcacgct ttccaggatc gtccgcagtc cgcgtgctcc ggtctttcgc
     2641 ttcatcgcac gtttcgctac ggcacgcaaa gcatcgtcgc ggaactcgag atccacgcct
     2701 tccatgtcga acagttttcg atactgcttg gtcagcgcat ttttcggttc gagcaggatg
     2761 ctaaccagcg cgtcctcgtc aagttcttca agcgtcgcaa cgaccggcag gcggccgacg
     2821 aattccggga tgagaccgaa gcggatcagg tcttccggct ctacatcatg cagcaattcg
     2881 ccgatgctct tttccatgtc gccggacttg acctcggcaa cgaagccgat gccacttttc
     2941 gacgaacggt tgagaatgat cttctcgagg ccggcaaagg cgccgccgca gatgaacagg
     3001 atattgccgg tgtcaacctg caggaattcc tgctgcgggt gctttcgccc tccctggggc
     3061 gggaccgagg cgatggttcc ctcgatcagt ttcagcagcg cctgctggac accttcgccc
     3121 gacacgtcac gcgtgatgga cgggttatcc gacttgcgcg aaatcttgtc gatctcgtcg
     3181 atgtagacga taccggtctg ggctttttcg acgtcgtagt cgcacttctg caggagcttc
     3241 tggatgatgt tctcgacatc ctctccgacg tagccggctt ccgtcagcgt cgttgcatcg
     3301 gcaattgtga acggcacgtt cagcaaacgc gcaagcgtct ccgcgagcag cgtcttgccg
     3361 caacccgtgg gg
//
LOCUS       DASUOM010000121         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_980605,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000121 DASUOM010000000
VERSION     DASUOM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980605"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFY27_02430"
     CDS             <1..636
                     /locus_tag="VFY27_02430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5764395.1"
                     /translation="IEQYESLLTDNRIWKQRTVNIGVVSPERALNLGFTGPMLRGSGI
                     EWDLRKKQPYAVYEEMDFDIPVGVNGDCYDRYLVRIEEMRQSNRIVQQCIDWLRRNPG
                     PVMLDDHKITPPSRVAMKDDMESLIHHFKLFTEGYTVPEGEVYAAVEAPKGEFGVYLV
                     SDGANKPYRLKVRAPGFAHLSAMSEMVEGHMLADVVAVIGTQDIVFGEIDR"
     gene            633..1124
                     /locus_tag="VFY27_02435"
     CDS             633..1124
                     /locus_tag="VFY27_02435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5764396.1"
                     /translation="MSELQPLSEHVLAEIDRWRARFPEGRQRSAVIGALHAVQHENGG
                     YVTEELMDRVAEYLELPKINVYEVAAFYSMFQTNAVGRHNIAICTNVSCMLRGAEEIV
                     AHVEQKLGIELGQSTADGRIYLKKEEECLAACCGAPMMMVDHKYHENLSIEKVDEILD
                     ALD"
     gene            1139..>2376
                     /gene="nuoF"
                     /locus_tag="VFY27_02440"
     CDS             1139..>2376
                     /gene="nuoF"
                     /locus_tag="VFY27_02440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5764397.1"
                     /translation="MDIQQNLVCFSTFGEEQPWTLESYEKHGGYQAWRRILAGELTPE
                     AIIEEVKASGLRGRGGAGFPTGLKWSFMPRNAPGQKYLVCNSDESEPGTCHDREVLRY
                     NPHSLVEGMAIASYAMGVTVAYNYIRGEFIDEPVPRFENALKEAYEAGLLGKNIQGTG
                     IDVDIHTFVGAGAYICGEETALLESLEGKVGMPRFKPPFPANFGLYGRPTTINNTQSL
                     ASVPAIIRNGAAWFAGLGPEGSGGTAQFSVSGHVEKPGNFELPMGIPFRDLLELCGGV
                     LGGRKLKAVIPGGSSVPVLPADIIMNCTMDYNSLKEAGSSFGTGAVMVMDETTCMVRT
                     LRRIARFYMAESCGQCTPCREGTGWLYRMLSRIVQGRGTEEDLDKLKQVADKIEGHTI
                     CALGDAAAWPVQSFLRHFRH"
BASE COUNT          494 a          687 c          750 g          445 t
ORIGIN      
        1 atcgagcagt acgaatcgct gctgaccgac aacaggatct ggaagcagcg taccgtaaac
       61 atcggagtgg tctcgcccga gcgtgcgctc aatctcggat tcaccggccc tatgttgcgc
      121 ggttcaggca tcgaatggga cctgcgcaag aaacagccct acgccgtgta cgaggagatg
      181 gatttcgata ttccggtggg cgtcaacggc gactgctatg accgttacct ggtgcgtatc
      241 gaggagatgc gccagtccaa tcgcatcgtt cagcaatgca tcgactggct gcgccggaac
      301 ccgggccccg tgatgctcga cgatcacaag atcacgccgc cgagcagagt tgcgatgaag
      361 gatgacatgg aatcgctcat ccatcacttc aagctgttta ccgaaggcta tacggtgccc
      421 gagggcgagg tatatgccgc tgtcgaggcg ccgaagggcg agttcggcgt atacctggtc
      481 tccgacggcg ccaacaagcc ttaccggttg aaggttcgcg cgcccggatt cgcgcacctg
      541 tcggccatgt cggagatggt tgaggggcac atgctggccg atgtcgtcgc cgtaatcggc
      601 acgcaggaca tcgtttttgg cgagatcgac cggtgagtga gctccagccg ctttccgaac
      661 atgtgctcgc ggaaatagac cgctggcgcg cgcgctttcc ggaaggccgg cagcgctccg
      721 ccgtcatcgg ggcgctgcac gcggtacagc acgaaaacgg cggctatgtc accgaagaac
      781 tgatggatcg tgttgccgag tacctggagc tgccgaagat aaacgtctac gaggtggcgg
      841 ccttctactc gatgttccag acaaacgccg tgggccgcca caacatcgcc atttgcacca
      901 atgtctcttg catgttgcgt ggtgccgaag agatcgttgc acacgttgag caaaagctcg
      961 gcatagagct cgggcagagc accgcggacg gtcgcatcta cctgaagaag gaagaagagt
     1021 gccttgccgc ttgctgtggt gccccgatga tgatggtcga ccacaagtac cacgagaatc
     1081 tcagcatcga aaaagtggat gaaattctgg atgcactgga ctgatggatg cccggctgat
     1141 ggatatacag cagaatctcg tgtgcttcag cactttcggc gaggaacagc catggaccct
     1201 ggagtcgtat gagaagcacg gcggttacca ggcatggcgc aggatactgg ccggcgaact
     1261 gacgccggaa gcgatcatcg aggaagtgaa ggcatcgggt ttgcgcggcc gcggcggcgc
     1321 tggctttccc accggcctca agtggagttt catgccgcgc aacgcgccgg ggcagaaata
     1381 ccttgtgtgt aattcggacg agagcgagcc gggcacgtgt catgaccgtg aggtgctgcg
     1441 ttacaacccg cattcgctgg tcgaaggcat ggccattgcg tcttatgcca tgggcgtcac
     1501 cgtcgcctat aactacattc gcggcgaatt catcgatgaa ccggtgccac gcttcgagaa
     1561 tgcactgaaa gaagcttacg aggcgggact gctcggcaag aacatccagg gaacgggcat
     1621 cgacgtcgac attcatacat tcgtcggtgc cggcgcctat atctgcggcg aggagaccgc
     1681 cttgctcgag tcgctggaag gcaaggtagg catgcctcgg ttcaagccgc cattcccggc
     1741 gaacttcggc ctgtatgggc gccccacgac catcaacaac acccagagtc tcgcgagcgt
     1801 cccggcaatc atccgtaacg gcgcggcgtg gtttgccggt ctcggccccg aaggttcagg
     1861 cggcaccgcg cagttttccg tttccggaca tgtggagaag cccggaaact tcgagttgcc
     1921 gatgggcatc ccgtttcgcg acctgctgga actctgcggc ggcgtcctgg gaggccgcaa
     1981 actgaaggcg gtcattccag gcggctcgtc cgttccggtt cttccagcgg acatcatcat
     2041 gaactgcacc atggactaca actcgctcaa ggaggccggc tcctcgttcg gcaccggggc
     2101 ggtgatggtg atggatgaaa ccacttgcat ggtacgcacg ctgagacgca tcgcgcggtt
     2161 ctacatggcc gagtcctgcg gccagtgcac gccgtgccgc gagggcaccg gctggctcta
     2221 ccgcatgcta agcaggatcg tccaggggcg cggcaccgaa gaggatctgg acaagctgaa
     2281 acaggtcgcg gacaagatag agggacacac catttgtgcg ctcggcgacg ccgcggcgtg
     2341 gccggtacag agtttcctcc gccacttccg ccacga
//
LOCUS       DASUOM010000122         7265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1582532,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000122 DASUOM010000000
VERSION     DASUOM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582532"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..2131)
                     /locus_tag="VFY27_02445"
     CDS             complement(68..2131)
                     /locus_tag="VFY27_02445"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX5764398.1"
                     /translation="MFTKTVLAGALVLLGTATIAGPAVMDPVHEGASAASADWPVNGG
                     NHRGEHFSVLDQVNVKSVAQLGLAWATEIPAADGISATPIVIDGVVYVSGPFSMVFAL
                     DAGTGKMLWTYDPDVRKSFASIPHLSWTARSNRGVAVGDGLVVVAVADCRLIALDAAS
                     GRPAWTAATCDPSQGYAITDAPRIGGGKVFIGNAGSESGMRNRGYVSAYNLRDGELLW
                     RFFTVPSPDPAENDSAAMKMAAATWTGDAWQQFGGGGSVWNEMTYDPDSNYLFFGTAG
                     ALPYVHRYRSPQGGDNLFLSTVLAVDSETGEYVWHYQTVPEDSWEYNATMNIVLADLE
                     IGGRQRSVAMIAPKNGFYYVLDRLTGEFISAKNYVKVNWASHINAETGRPVIDPAGQF
                     WNAAEGVRTELWPNMWGSHSWNPMAYHPGLQLAYIPAIDLPSIVTNHGNGDFTDSLEM
                     VTEAGGEAHSPGKLIAWDPVRQMARWTADHSLPFNGGVLATGGGLVFQGDATGDFTAY
                     DAADGEQLWSVQTGSAINAAPVSYLHGGKQYVLVPIGAGGGAQFVYPELHASEQGHGR
                     GRLLAFSLDGRLELPQETAAVRQLSELRTTAADAVVQRGEELYRDSCRYCHGKEVVAR
                     AGGSVPDLRFATEETHAAWNAIVVGGAKRLNGMPGSEMSREDAEAVRQYVLSKTDELR
                     ERLRH"
     gene            complement(2192..2623)
                     /locus_tag="VFY27_02450"
     CDS             complement(2192..2623)
                     /locus_tag="VFY27_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764399.1"
                     /translation="MNAEELQTHWALLLASVTSLVLLLFAAAHFMQTSARGQLRRTQK
                     ALQQERGRLEKAKAVVDKAERQKERLMRNAESVKPRLLRESAEACEDAQALVRIAADR
                     VLVAENHLRRVILEEFPPAQHETLRSRYLPDAPEKKKPFTF"
     gene            complement(2648..3025)
                     /locus_tag="VFY27_02455"
     CDS             complement(2648..3025)
                     /locus_tag="VFY27_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX5764400.1"
                     /translation="MEERPRDHVPGDEPPRDHVQEDDTASTREPRDFEEHIKSRTTWL
                     RLLFMIIFVFLYGLSRIVVLAVVVLQFLWVLFTGETNDKLKTLGQSLATYTYQLIRYL
                     TYNSDSRPFPFDDDWPSPVAPPL"
     gene            complement(3143..3427)
                     /locus_tag="VFY27_02460"
     CDS             complement(3143..3427)
                     /locus_tag="VFY27_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HEX5764401.1"
                     /translation="MKAKTSIITENDLPPEVIRAIEQGRKIEAIKLLREATGLGLANA
                     KVLVDKAGRQHAPRNVQPRLVEDQPNLGRLLKTLFFVALAFAFYRYYTGA"
     gene            3615..5009
                     /locus_tag="VFY27_02465"
     CDS             3615..5009
                     /locus_tag="VFY27_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5764402.1"
                     /translation="MKRSTMLAIACLAVGACDEGRDVDGSKDKSTADSPTPYASKYTP
                     LPSDATLLTRATVLTGTGERLDSADVLIRDGKIAAVGTELDAGGAEVVDAGGMWITPG
                     IIDVHSHLGVYPSPGVDAHGDGNEISAPNTAEVWAEHSVWPQDPGFVTALQGGITTLQ
                     ILPGSANLFGGRSVTLKNVPGRSVQDMKFPGAPYGLKMACGENPKRLYGSKGSAPSTR
                     MGNVAGYRKAWIAAEDYRDKLGKADDENGDAEPPTRDLQLETLVGVLNGEILVHNHCY
                     RADEMAVMIDVAKEFGYRIAAFHHAVEAYKIPDLLAENDICAAVWADWWGFKMEAFDG
                     IRENAAIVADAGACAIVHSDDQRGIQRLNQETAKVMGAAARAGIDIAPEQAIRWITSN
                     AARALGIGESVGTLEPGKMADIAVWDGNPFSVYSKVQRVLIDGALVFERGNAEVNPVT
                     DFSLGTAATTGGAK"
     gene            5006..6310
                     /locus_tag="VFY27_02470"
     CDS             5006..6310
                     /locus_tag="VFY27_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764403.1"
                     /translation="MSPLFKAILLVASFLFTNTLAAQTIAVKGATVHTAGPSGKIEKA
                     TIIISDGRITAIGAGVATPAGAREIDASGKIITPGLFTPFGQIGLVEVDAVEGTVDYV
                     QRGDLFSASFDPASAYNPRSGLVATNRIEGITHAVIAPGAQEPDEFGNQSRVLSGLAS
                     VVQLSDDDYMVERRAALVANLGETGSQVAAGSRATALMVLRTALDDASDYARNVQAFN
                     QGNRRQYSLSRSDLEALQDLLEGEMPLVVRANRANDISSAIRLAADYELDLIVMGGVE
                     AWMVADELAAAGASVILDSLANLPDSFDQLNARLDAASILAKAGVRIAIGGDGANQNH
                     NARNITQAAGIAVAHGLSWDDALAAVTLAPAQMYGVADRIGSLEAGKRADFVIWNDDP
                     LELTSYPDQVFIGGKAVPMESRQTLLRDRYLDHSSNKPPAFR"
     gene            complement(6314..6387)
                     /locus_tag="VFY27_02475"
     tRNA            complement(6314..6387)
                     /locus_tag="VFY27_02475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6353..6355),aa:Gly,seq:ccc)
     gene            6434..>7265
                     /locus_tag="VFY27_02480"
     CDS             6434..>7265
                     /locus_tag="VFY27_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5764404.1"
                     /translation="MSRSPKVHRRQFLKLTAAAGVALSLPADGQATSPRMDRRKIPGT
                     GDAIPVVGLGTSHEFDHIPADGGKELKAVLTTLVEHGGSLIDTAPRYGNAEEVIGGFL
                     AEMDLVDTLFVSTKVSTRGERQGRQSLERSHRLLGKRPLDLMMVHSLVDAETNLRILG
                     EWKDAGHVRYIGITTSERNAHGEMESLILSHDLDFVQVNYSPLEPEAAERVIPAAADR
                     GVAVMINRAFGNGEYFSRLRGRELPGWAEEFGCESWAQFSLKYILGHPDVTCVLAATS
                     S"
BASE COUNT         1424 a         2245 c         2233 g         1363 t
ORIGIN      
        1 gtaagcagcg ggttgcgcca ataaagttct gcaaggaagc ttcgtcgcat cggcaggcgc
       61 ccgcgtgtca gtgtcgcagc ctttcccgga gttcgtctgt tttgctcaac acatactggc
      121 gaacggcttc tgcatcttcc cggctcatct ccgaacccgg catgccgttc aggcgcttcg
      181 cacccccgac tacgattgca ttccacgcag cgtgggtttc ttcagtcgcg aaccgaagat
      241 ccggcaccga gcccccggcg cgcgccacca cctcctttcc gtgacaatag cggcaactat
      301 cgcggtaaag ctcctcgcct cgctgcacca cagcatccgc agcagttgta cgaagttccg
      361 aaagctgtcg caccgccgca gtttcttgcg gcagttcgag gcgcccatcg agggaaaaag
      421 ccagcaggcg tccgcgccca tgaccttgtt cggaggcatg cagttccgga taaacgaact
      481 gtgctccccc acccgccccg atgggcacga ggacgtattg cttccccccg tgcaagtagc
      541 ttacaggcgc cgcatttata gcggagcctg tctgaaccga ccacagttgc tcgccgtcag
      601 ctgcatcgta cgcagtaaag tcgccggtcg catctccctg gaatacgagc ccgcctccgg
      661 tggccagtac gccgccgttg aacggcagcg agtgatcggc agtccaccgc gccatctgcc
      721 gaaccgggtc ccaggcaatg agcttgccgg gactgtgcgc ctcgccgcct gcttccgtga
      781 ccatctccaa tgaatccgtg aaatcgccgt tgccgtggtt ggtgacgatg ctcggtaaat
      841 cgatcgcggg aatgtacgca agctgcaggc cggggtgata tgccatcggg ttccagctgt
      901 gcgagcccca catgttcggc cagagttcgg tacgaacgcc ttcggccgca ttccagaatt
      961 gccctgcagg atcgattacc ggccggcccg tctcggcatt gatgtgcgac gcccagttga
     1021 ccttcacata attttttgcc gagatgaatt cgccggtcag cctgtccagc acgtagtaga
     1081 aaccgttctt cggcgcgatc atcgcgacgg accgctgtct gccgccgatc tcgaggtcag
     1141 cgagcacgat gttcatcgtc gcgttgtatt cccagctgtc ttcgggaacc gtctggtaat
     1201 gccatacgta ctcgccggtc tcggaatcca cggcaagcac cgtcgacagg aacaggttat
     1261 cgcctccttg cggactgcgg tagcgatgca cgtacggcaa tgcgccggct gtgccgaaga
     1321 agaggtagtt gctgtccggg tcgtaggtca tttcattcca gacgctgccg ccgccgccaa
     1381 attgctgcca tgcgtccccg gtccaggtgg ccgcggccat tttcatggcg gccgaatcgt
     1441 tttcggctgg atcggggctg gggacggtaa agaagcgcca cagcagttcc ccatcgcgca
     1501 ggttgtacgc cgagacgtag ccgcggtttc gcattcccga ctcggagccg gcattgccga
     1561 tgaacacctt gccgccgccg attcgcggag catccgtgat ggcatagccc tgtgacggat
     1621 cgcaggtcgc cgctgtccag gccggcctgc cgctcgcggc atcgagcgcg atgagcctgc
     1681 aatccgccac ggcaacgacg acgagtccgt cgccgaccgc gactccccga ttggaccggg
     1741 ccgtccagga aaggtgtggg atagacgcaa aggatttcct gacgtcggga tcgtacgtcc
     1801 acagcatctt tccagtcccg gcatcgagtg cgaagaccat cgagaacggc ccggaaacgt
     1861 agacaacacc atcaatcacg atcggcgttg cggagatacc gtctgcggca ggtatctctg
     1921 tggcccacgc caggccgagt tgtgctaccg acttcacatt gacctggtcg agcacgctga
     1981 aatgttcgcc acggtgattg ccgccgttca cgggccagtc cgcggacgca gccgatgcgc
     2041 cttcgtgcac tggatccatg actgcgggac cggcaatcgt cgccgtgcca agcagaacaa
     2101 gcgcgcctgc aagaactgtt ttagtgaaca tctgtccctc gcccgtgcga gcagcgatta
     2161 tacgatccga cccggctgct tcatgcgggg atcagaatgt gaatggcttc tttttttccg
     2221 gtgcgtcagg caggtagcgg ctgcgaagag tttcatgctg cgccggcgga aactcctcga
     2281 ggatgacacg gcgaaggtga ttctccgcaa ccagtaccct gtcggcagcg atcctgacca
     2341 gcgcttgcgc gtcttcgcac gcctccgcag attcgcggag caggcgtggc ttgacgctct
     2401 cggcgttccg catcaagcgt tctttctgac gttcggcttt gtcgaccact gctttcgcct
     2461 tctcgagcct gccgcgctcc tgctgcaggg ctttctgcgt gcggcgcaac tgccctcgag
     2521 ccgaggtctg catgaaatgt gcggcggcaa atagcagcag gaccaggctg gtcacggatg
     2581 ccagcagcag ggcccagtgc gtttgcaact cctccgcatt catctgaata cggccagcca
     2641 ccaaaatcta gagtggtggc gcgaccggcg atggccaatc gtcgtcgaaa ggaaagggtc
     2701 tcgagtcgct gttgtaagtc aggtaacgga ttagctggta ggtataggta gcaagcgact
     2761 ggcccaaagt cttgagttta tcgttcgtct caccggtgaa caggacccac aggaactgca
     2821 gcacgacgac tgccagcacg acgatcctgg acagaccgta caggaacacg aagatgatca
     2881 tgaagagcag gcgaagccag gtggtcctcg acttgatgtg ctcttcgaag tctcgcggct
     2941 cccgcgtcga tgccgtgtca tcttcctgga catggtcccg tggcggttcg tctccgggaa
     3001 catgatctct cggccgttct tccatcgctg caccccgtta acagagcctg tcccggtgcc
     3061 ctggcgtggc attcgaaggg taccgggggc ggtatgataa ccttttcggc gaaagctgta
     3121 ttccgcacct gccgataccg gatcaggcgc ctgtgtagta gcggtagaac gcgaacgcca
     3181 gcgcgacgaa gaacagggtc ttgagcagcc tgcccaggtt cggctgatct tccacgagcc
     3241 gcggctgaac gttgcgggga gcatgctgtc gtcccgcctt gtcgacgaga accttcgcat
     3301 tggcgaggcc gagaccggtt gcttcgcgca gtaatttgat ggcttctatc ttgcgacctt
     3361 gttcgatcgc gcggataacc tcaggtggca ggtcgttctc ggtgatgatg gatgttttcg
     3421 ctttcatctc gtcagttcgt gtatccggtt gccaattgcc gattgctcca gcacgtcata
     3481 aggataaccc gtcgagccgt gtctgcacac tgagccatgt ctcaaattgg cgcggggcac
     3541 ggcttctctg tgattttccg tcacacgccc gagcatgtat agttcgggca cgaaatcggc
     3601 accggaggat ctggatgaag agaagcacga tgctggcaat cgcctgcctg gctgttggcg
     3661 cctgcgacga gggtcgcgac gttgacggca gcaaagacaa gagcacagcc gattctccga
     3721 ctccatatgc cagcaagtac actccccttc cctccgacgc cacgctgctg acccgggcga
     3781 ccgtcctcac cggcaccggc gaacgactcg attccgctga cgtgctgatc agggacggaa
     3841 agatcgccgc agtcggcacg gaactcgacg ccggcggtgc cgaggtcgtc gacgccgggg
     3901 gtatgtggat cacgccgggg atcatcgacg tacattctca ccttggtgtc tacccttcgc
     3961 cgggagttga cgctcacggc gacggtaacg agatatcggc gccgaacaca gccgaggtat
     4021 gggcggagca cagcgtctgg ccgcaggacc cgggtttcgt caccgcgcta cagggcggca
     4081 ttaccacgct gcagattctc cctggatccg cgaacctctt cggcggccgc agcgtcaccc
     4141 tgaagaacgt gcccggccgc tctgtgcagg acatgaagtt tcccggcgca ccttacgggc
     4201 tgaagatggc ttgcggcgaa aatccgaaac gtttgtatgg cagcaagggt agcgcaccgt
     4261 cgacgcgcat gggaaacgtc gcgggctatc gcaaagcctg gatcgccgca gaggattacc
     4321 gcgacaagct cgggaaggct gatgacgaga acggtgacgc tgagccgccg acgcgcgacc
     4381 tgcagctcga gacactggtc ggcgtgctga acggcgagat actcgtgcat aaccattgct
     4441 accgcgccga cgaaatggcc gtgatgatcg acgttgcgaa agaattcggt taccgcatcg
     4501 ccgcctttca ccacgcggta gaggcctaca agatcccgga cctgcttgcg gagaacgata
     4561 tttgcgccgc cgtctgggcc gactggtggg gcttcaagat ggaggcgttc gacgggattc
     4621 gcgaaaacgc tgcgatcgtt gccgacgccg gcgcctgcgc catagtgcat tccgacgacc
     4681 agcgaggcat ccagaggctc aaccaggaaa cggcgaaagt catgggagcc gcggcgcgtg
     4741 ccggtatcga catcgccccg gaacaggcga tccggtggat tacgagcaat gccgccagag
     4801 cgctcggcat tggcgagtcc gtcggcacgc tggagccggg gaagatggcg gatatcgctg
     4861 tgtgggacgg caacccgttc agtgtctatt ctaaagtgca gcgcgtattg atcgacggtg
     4921 cgctggtctt cgaacgcggc aatgcggagg tgaacccggt aacagacttc agtctcggca
     4981 cggctgcgac gacgggaggt gcgaaatgag cccgctgttc aaggcgatcc tgctcgtcgc
     5041 atcgttcctc ttcaccaaca ccctggccgc gcagaccatt gccgtgaagg gcgctacagt
     5101 gcacaccgcg ggaccctcgg gcaagatcga gaaggccacg atcatcataa gcgacggccg
     5161 cataacggcg atcggcgcag gcgtcgcgac tccggcgggg gcgcgcgaga tcgatgcgtc
     5221 gggcaagatc attacccctg gcctgttcac acccttcggc cagatcggtc tcgtcgaggt
     5281 ggacgcggtc gaaggcacgg tcgattacgt tcaacgcggc gacctgttct ctgcaagctt
     5341 cgacccggct tcggcataca acccgcgctc cgggctggtc gccaccaatc gcatcgaagg
     5401 catcacgcat gcggtcatcg cgccgggagc acaggaaccc gacgagtttg ggaaccagag
     5461 ccgcgtcctg tccgggctgg catccgtcgt gcaactgagc gacgacgatt acatggtcga
     5521 acgccgcgcc gcccttgtcg ccaacctggg cgagaccggc agccaggtgg cggcgggttc
     5581 gcgggcaacg gcgctgatgg tgttgcgcac ggcactggac gacgcgagcg actatgcgcg
     5641 caacgtgcag gccttcaacc agggcaaccg gcgtcagtac tccctgagcc gcagcgatct
     5701 cgaggcgctg caggatttgc tcgagggcga gatgccttta gtggtcaggg cgaacagggc
     5761 gaacgacatc tcttccgcta tccggctcgc cgcggattac gagctcgacc ttatcgtgat
     5821 gggcggagta gaagcctgga tggtagccga cgagctggcg gcagccggag ccagcgtcat
     5881 tctagactcg ctcgcgaacc tgccggacag cttcgaccag ctcaacgcaa gacttgacgc
     5941 tgcctcgata ctcgccaaag cgggagtgcg tatcgcgatc ggtggagacg gcgccaacca
     6001 gaaccacaac gcccgaaaca ttactcaggc ggctggcatt gccgtagcgc atggcctgtc
     6061 atgggacgac gccctggccg cggtgacact ggcgccggcg caaatgtacg gagttgccga
     6121 tcgcatcggc agcctggagg ccgggaagcg cgccgacttc gtaatctgga atgacgatcc
     6181 gctggaactg acgagctacc cagaccaggt tttcattggc ggcaaagccg taccgatgga
     6241 aagccgccag acgctgctcc gggaccgcta cctcgaccat tcgtcgaaca agccgccggc
     6301 gtttcgctaa agctggagcg ggtgaagggg atcgaaccct cattcttagc ttgggaagct
     6361 aatgttctac cattgaacta cacccgcagc gatccaggag tctggctgcg gcaacactcg
     6421 taacggagcc gacatgagca gatccccgaa agtccaccgc cggcagttcc tgaagctcac
     6481 cgccgctgcc ggtgttgcat tatcgttgcc cgccgacggc caggcaacat ctcccaggat
     6541 ggacaggaga aaaattcccg gaaccgggga cgccattccc gtggtcgggc tcggcacatc
     6601 gcatgaattc gaccacatac ccgcggacgg cggcaaggaa ctcaaggcgg tcctcaccac
     6661 actggttgag cacggcggct cgctgatcga tacggcgccg cgctacggga atgccgaaga
     6721 ggtcatcggc ggattcctgg ccgaaatgga tctcgtcgat acgctcttcg tttcgacgaa
     6781 ggtcagcacg aggggagaaa gacagggccg gcagtccctg gaacgatccc atcggttact
     6841 cggcaagcgc cctctcgacc tgatgatggt tcacagcctg gtcgatgccg agaccaatct
     6901 ccgcattctt ggcgagtgga aagacgccgg tcacgtacgg tacatcggca tcactacgtc
     6961 agagcgaaac gcgcacgggg aaatggaatc actgatactg tcgcacgacc tggacttcgt
     7021 gcaggtgaat tactcgccgc tcgaacccga agcagcagaa cgcgtgattc cggccgccgc
     7081 tgaccggggc gtcgccgtca tgatcaatcg cgcgttcggc aatggagaat atttcagccg
     7141 gttgcgcggc cgggaactgc cagggtgggc agaagagttc gggtgcgaat cctgggcaca
     7201 gttttccctg aagtacatcc ttggccaccc ggacgtcacc tgtgtgcttg ccgccacgag
     7261 cagct
//
LOCUS       DASUOM010000123         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1711171,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000123 DASUOM010000000
VERSION     DASUOM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711171"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="VFY27_02485"
     CDS             complement(<1..1731)
                     /locus_tag="VFY27_02485"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764405.1"
                     /translation="MRQLQIFISSPGDVFEERAIAQRVIERLQSEYAGRVALKPVLWE
                     HEPLLASSTFQEQIIRPSETDVVVSILWSRLGTRLPKGFTREDGSRYDSGTEYEFEDA
                     ITGFRKNGKPDLLVYRKTAPPSVRLDDEKELLERLQQKKKLDEFVNRWFHDKAEGTLI
                     AAFHAFDSPSDYESLLEAHLHKLIERKLPDTGAAAGEAPAVWKKGSPFRGLHAFEFEH
                     APVFFGRTKAVSDILQGLRNQAADGRAFVLVLGMSGGGKSSVVRAGVLPMLTQPGVIE
                     GVALWRRAIFRPADTPGDLFTGLASALLRDQALPSLDPENEGPASLARVLRDSPQSAV
                     MLIKAALEQAAAGQSKSGTKKIQSRLALVIDQMEEMFTQEGITAKERKAFADTLDALA
                     RCGRVWIVGTLRSDFYPRLMDLPVIGALKDGDGQYDLMPPVASEIGQMIRLPTRAAAL
                     RFEEDFASSERLDDILRDAAAERPEILPLLQFTLEELYQRRTPNGTLTLEAYRELGGV
                     EGSLAKRAETVFQELPPEVQAALPKVLNALVSVGQDGHKTIGRKRAPWKNATNAEVRK
                     LLETFVAARLF"
     gene            complement(2014..>2589)
                     /locus_tag="VFY27_02490"
     CDS             complement(2014..>2589)
                     /locus_tag="VFY27_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5764406.1"
                     /translation="EALAVWAFRSRVLDYLVKPVSRTDFQRALEALRRMSDIGPSQRG
                     RTPLNLAAPIPPDIPRTPRPDNVALMPSIYFVKQRFREKIRCEEVAASCGMGTFRFSR
                     AFHETFGLTFQDYLLRYRILQACRLLRHPEGNVTKVAYSVGFNDASYFSRAFRRFIGT
                     SPSSYAAEVGPGDSESDISSIQLMLKLPDAG"
BASE COUNT          472 a          795 c          828 g          494 t
ORIGIN      
        1 gaacaggcga gcggcgacga aagtctccag cagtttgcga acctccgcgt tcgtcgcgtt
       61 cttccacggg gcacgctttc ggccgatggt cttgtggccg tcctgcccga cgctgaccag
      121 cgcgttgagc accttcggca atgccgcctg aacctccggc ggcagttcct ggaacaccgt
      181 ctcggcacgt ttggcgagcg acccttcgac gccgcccagc tcccggtaag cctccagggt
      241 cagggtgccg tttggcgtgc gccgctggta aagctcttcc agcgtgaact gcagcagcgg
      301 cagtatctca ggacgctctg ccgcggcgtc acgaaggatg tcatcgagac gctcgctgct
      361 cgcgaagtct tcctcgaagc gcaaggcggc agcacgcgtc ggcaggcgta tcatttgccc
      421 gatttcgctg gcgaccggtg gcatgaggtc gtactggccg tcgccatcct tcagcgcccc
      481 gatcacagga aggtccatca agcggggata gaagtcgctc ctcagcgtac caacgatcca
      541 tacccgtccg cagcgagcca gggcgtcgag cgtgtcggca aaggcttttc gttcttttgc
      601 agtaatgcct tcctgggtga acatctcctc catctggtcg atgacgagcg ccagcctgga
      661 ctggatcttc ttcgtgccgg acttggattg tcccgccgct gcctgctcga gtgcggcctt
      721 gatcaacatc accgccgact gtggcgaatc gcgcaggacg cgtgcaaggc ttgccggacc
      781 ctcgttttcc gggtcgagtg acggcagcgc ttggtcccgg agcagggcgg acgccagccc
      841 tgtaaacagg tctcccggcg tgtcggccgg acggaatatc gcgcgccgcc acaaggcaac
      901 gccttcgata acgccaggct gcgtcaacat cggcagcacg ccggcccgta ccaccgaaga
      961 cttgccgccg ccgctcatgc cgagcacgag cacgaaagca cggccatccg cagcctggtt
     1021 cctcaggccc tgcaggatgt cggataccgc ttttgtacgg ccgaagaaca cgggggcatg
     1081 ctcgaattcg aacgcgtgca ggccgcgaaa gggcgagccc ttcttccaca cggccggcgc
     1141 ttcgccggca gcggctccgg tatccggcag cttgcgctct atgagcttgt ggagatgtgc
     1201 ctccagcagg ctttcgtaat cacttggtga atcgaaagca tggaaggcgg cgatgagtgt
     1261 gccttcggcc ttgtcgtgaa accagcggtt gacgaactca tcgagcttct tcttttgttg
     1321 cagtcgttcg aggagttcct tctcgtcgtc cagccgcacc gagggcggag cggttttgcg
     1381 gtacaccagc agatcgggct tgccgttctt gcggaagccg gttatcgcgt cctcgaactc
     1441 gtactcggtg ccggagtcgt agcgggagcc gtcttcgcgc gtgaagccct tcggcaggcg
     1501 agttcccagc cgcgaccaga ggatcgagac gaccacgtcc gtctcggacg gccggatgat
     1561 ttgctcctgg aaagtcgagg aagcgaggag cggttcgtgt tcccagagga ccggcttcaa
     1621 cgcgacacgg cctgcatatt cgctctgcaa acgctcgatg acgcgctggg cgatagcgcg
     1681 ctcctcgaac acgtcgcctg gagacgagat gaagatctgt agctgtctca ccgtggacct
     1741 gctaatacat tcttgtagaa cgacaagccg ccgaccaagc gaggatatcg ttaataacaa
     1801 ttagtggcgt cggggtcgcg gcaaacgcca catatggtcg ctggcattga tgcggtgggt
     1861 tcttgcgccg cgggcccttg cccccaagcc gcgtaccgca ccaggccggt gcaaaacgga
     1921 gtatttatag cctatttcgg ggacttttgc gcgaactagt tcggctatgc ccggggcgca
     1981 aaggcagtct tcgccagggc cgaccgggca gcgttaaccg gcgtccggca gcttgagcat
     2041 cagttggatc gaggaaatat ctgactcact gtcgccaggt cccacctctg cggcgtacga
     2101 agacggtgag gttccgatga agcgccggaa cgcgcgggaa aaataggagg catcgttgaa
     2161 gccgaccgag taggcgactt tggtgacgtt cccctccggg tgccgcagca gccgacaggc
     2221 ctggaggatg cgatagcgca ggaggtagtc ctggaacgtg aggccaaagg tttcgtgaaa
     2281 cgcgcggctg aagcggaagg tgcccatacc gcagctcgcg gccacctcct cgcagcggat
     2341 cttttcgcga aacctctgtt tgacgaagta aatcgaaggc atcagtgcga cgttgtccgg
     2401 tcgtggtgtg cgaggaatgt ccggcgggat gggcgccgcc aggttcaggg gagttcgtcc
     2461 tcgctgggag gggccgatgt ccgacatgcg gcgcagcgcc tccagtgctc gctggaaatc
     2521 ggtgcgggac accggcttca ccaggtagtc gagcacccgg ctccggaacg cccagactgc
     2581 cagcgcttc
//
LOCUS       DASUOM010000124         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_83798,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000124 DASUOM010000000
VERSION     DASUOM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83798"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFY27_02495"
     CDS             complement(<1..255)
                     /locus_tag="VFY27_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764407.1"
                     /translation="MEYDELSQSGIRRIANYSPYLDQLAGLASEMGLRQRSNLVHLER
                     CLNQEWSLGENSILSWAQLDDGILILVVPHYAIAEYTASPD"
     gene            536..1924
                     /locus_tag="VFY27_02500"
     CDS             536..1924
                     /locus_tag="VFY27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5764408.1"
                     /translation="MLLDRLKSIVGNSGWTSDPGILEPHLTEWRDRYRGSTPIMVSPA
                     TTEQVAEVVRACAEAGNPIVPQGGNTGLCGGAIPDNSGREILLSLGRLNKIRSISALD
                     YSMVAEAGCILATIQSAAAGVDRLFPLSLAPEGSCQIGGNLSTNAGGINVLRYGTARD
                     QALGLEVVLPDGSVWDGLRALRKDTAGYDLKQLFIGAEGTLGIITAATLRLYPSVRNV
                     QTAFVGVASVADAISLLSYVREEASDRLQAFELIQHRALQFVVDHFPALRNPFQPGYP
                     WYVLMETANTPDAEAFEKLLLSAMEKSIVGDAVVAKSAAEATDFWSLRHSVSEAQKHE
                     GASLKHDIAVPIGQIGNFISAAESAVLERIPDARVVAFGHVGDGNVHFNISQPVSWQA
                     DQFLERRDEIAGLVYDATAAFNGSISAEHGIGRAKREDLRHYRSATELALMRALKKAL
                     DPADIMNPGKVI"
     gene            2028..>2757
                     /locus_tag="VFY27_02505"
     CDS             2028..>2757
                     /locus_tag="VFY27_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5764409.1"
                     /translation="MSANLDSIEQSFSDQLSKFDAKIKTQEDNASLLNEIHASLTVLI
                     AQSGDHDEEIREILQKRYNAGKLRLETYQLVRNVLDRVVIESMVTMPDAVDDDDTDSS
                     YRETTVVDGGSGEQEPRHEPLQIGSLLRDRFLLQERVSGGSMGVVYKAHDRRLAEADG
                     FDPWVAIKVLSPKLSRNAHALRALQQEAAKGRCLSHPNIVRFVDLDRDGDIYFIVMEW
                     LEGRSLAAILDENKNTRIDLATALD"
BASE COUNT          589 a          807 c          843 g          518 t
ORIGIN      
        1 gtccggggac gccgtgtatt ccgcaatggc gtagtgcggg acgacgagga tgagaatgcc
       61 gtcgtcaagc tgagcccagc tcaatatgct gttctcgccg agcgaccatt cctggttgag
      121 gcaacgctcc aggtggacca ggttgctccg ctgccgcagc cccatctcgc tggcgaggcc
      181 ggcgagctga tcgaggtacg gcgaataatt ggctattcgc cggatgccgg attgggataa
      241 ttcgtcatat tccatttgtc atcgcttaca acggcgcggg ggcgaggcat ccttgcccgc
      301 atggcgcttg tccgtggccg cagatccagc gggcgcacgc cgccaaaccc acggcccagc
      361 ggctcagcat acatcaggtc gagaccccgg acggcctttg ctattggcaa tattatgtgt
      421 accgttccgc caaagtaatc aactgcgcgt tgttgtgcaa gtgctgccca ggaaatcaac
      481 ctatgtcttt caaatcgctg ccaatgacca atcttcaggg aaggagactg acgtcttgct
      541 gcttgaccgg ctgaagtcca tcgtcggcaa ctcaggctgg accagcgacc cgggaatcct
      601 cgaaccgcat ctgaccgagt ggcgcgaccg ctaccgcggc agtacgccca tcatggtatc
      661 gccagccacc acggagcagg tggcggaggt ggttcgtgcc tgtgccgaag ccggcaaccc
      721 gatcgtgccg cagggcggga ataccggcct ttgcggcggg gcaattcccg ataactcggg
      781 aagggaaatc ctgctgtcgc tcggccgcct caataaaatc cggtccatct ctgcactgga
      841 ctattcgatg gtggcggaag ccggctgcat attggccacg atacaatcgg cggcggcggg
      901 ggttgaccgc ctttttccgt tgagcctggc tccggaaggc agttgccaga taggagggaa
      961 tctttcaacc aatgcaggcg gcattaacgt actgcgctat ggcaccgccc gggaccaggc
     1021 tttaggcctc gaagtcgtat tgccggatgg cagcgtctgg gatggcctgc gagcgttgag
     1081 gaaagacacg gccggttacg acctgaagca gctgttcata ggtgccgaag gtacgctcgg
     1141 catcattacg gcggcgacct tgcgccttta tccctccgtc cggaatgtac aaaccgcgtt
     1201 tgtcggtgtg gcaagcgtcg ccgacgcgat ctcgctgctc tcatatgtcc gggaggaagc
     1261 gagcgaccgt ttgcaggcgt tcgaactgat acagcatcgg gcgctgcagt tcgtcgtcga
     1321 tcatttcccc gctttgcgaa atccgttcca gccgggttat ccctggtatg tgctgatgga
     1381 gacggcgaac acccccgacg ccgaagcgtt cgagaaactg ttgctctcgg cgatggaaaa
     1441 atccattgtc ggggacgccg tcgtggcgaa aagtgcggct gaggcaactg acttctggag
     1501 ccttcgccac tcggtatcgg aagcgcagaa gcacgaggga gcgagcctca agcacgacat
     1561 agcggtaccc ataggccaga ttggcaattt catcagcgcg gcggaatccg cggtgctcga
     1621 gcgtatcccg gacgctcgtg tcgtcgcctt cggtcacgtg ggcgacggaa acgtgcattt
     1681 caacatcagc cagccggtat cctggcaggc ggatcagttc ctggaacgtc gcgacgagat
     1741 tgccgggctc gtctatgacg caacagccgc tttcaacggc agtatcagcg cggagcacgg
     1801 cataggcagg gcaaagcgcg aggaccttcg tcattaccga agcgcaacgg aactggcact
     1861 catgcgggcg ttaaaaaagg cgctggatcc ggctgatatc atgaaccccg gcaaggtgat
     1921 ttagcagcgt acagactgct cccgatgagg cacaatacgc cctcaggaaa ccgtcaaccc
     1981 ctttacagga agtggagaaa cacggccgca atgcggcctc tggaagcatg agcgccaatc
     2041 ttgacagcat cgagcagtcg ttttcggacc agttgtccaa atttgacgca aagataaaga
     2101 cgcaggagga caacgcgtcg ctgctgaacg agatacatgc atccctgacg gtgctgattg
     2161 cacagtcggg cgaccacgat gaagaaattc gcgaaatcct ccagaagcgc tacaacgccg
     2221 gcaaactgag actcgaaacc taccagcttg tcaggaacgt gctggatcgc gttgtcatcg
     2281 agagcatggt gaccatgccg gatgccgtcg atgacgacga caccgacagc tcgtatcgag
     2341 aaacgaccgt tgtcgacgga gggtccggcg aacaggaacc gcgccacgag cccctgcaaa
     2401 taggatcgct cctgcgcgac cgtttcctgc tccaggaacg ggtatccggc ggcagcatgg
     2461 gcgtcgttta caaagctcat gaccgtcgtc tcgcggaggc ggacggcttc gatccgtggg
     2521 ttgccatcaa ggtgctgtca ccgaagctgt cgcgcaacgc gcacgcgttg cgggccctgc
     2581 agcaggaggc agcgaagggg cgctgcctgt cgcacccgaa cattgtcagg ttcgtcgacc
     2641 tggatcgcga cggcgatatc tacttcatcg tcatggaatg gctcgaagga cgctcactcg
     2701 cggcgatcct cgacgagaac aagaacaccc ggatcgacct cgcgacggca ctcgaca
//
LOCUS       DASUOM010000125         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_825373,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000125 DASUOM010000000
VERSION     DASUOM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825373"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFY27_02510"
     CDS             complement(<1..980)
                     /locus_tag="VFY27_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764410.1"
                     /translation="MNLLNPLLLALILSAAITTSAAQSQEQHFHPKGKPPSTFTIELQ
                     DGLRATLPFEDRRDFEEAKRGFIAAPTYKQIKAEAGHVAWDMASYEFLLSDQNFASIH
                     PSLQRQAVLNMAYGLYEVLPERIYQVRGYDLANFTLVKGDTGWIVFDPLTAKETAKAA
                     LDFANDTLGKRPVVAVVYSHSHGDHFGGVRGVVEEADVRSGKVKIIAPVGFMDHAVSE
                     NVMAGTAMTRRMFYQYGVLLPRSPFGHIDQSIGKNTAVGNTGLIEPNVIIEKPFETLT
                     VDGVTMLFQNTPGTEAPAEMNTWFPQMKAFWAAENITGTIHNIYTLRGALV"
     gene            complement(1184..2182)
                     /locus_tag="VFY27_02515"
     CDS             complement(1184..2182)
                     /locus_tag="VFY27_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764411.1"
                     /translation="MRQVLPLVFLAAAGAVRAGALFDEEPVLDVRLAGPLHAVLESGA
                     ERMEFPFIVSVDGVDIPVEVRIRGNSRLRVCSFPPLRLDFPGKGTAGSVFDGQDKLKL
                     VTHCNDRKRDAGNLLDEYLAYRLFNLVSDDGYRVRLLRMAYVDTDSTGLAALVRDAFL
                     IESDEALAARRGAAVLDTPGVALSRLDGRQAALVYVFQYLIGNTDWSLVTAETADACC
                     HNVDLYERDGRTLPVPYDFDLAGLVHASYARPDASLKIASVRTRRYRGYCISSDDLAA
                     AIDAVTLREADILALAAELPAASEEARRKRREYLGEFFADAADREELLEQFEKRCL"
     gene            2295..>3073
                     /locus_tag="VFY27_02520"
     CDS             2295..>3073
                     /locus_tag="VFY27_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764412.1"
                     /translation="MKHVASLLCACALIAGGHPARAGDPVLNELEYFQSGSVDYLVFS
                     NWYDGLFSDSKISGIELIHHGERTATNGDVRLDATPEQWDRIPEFVEKKVDRERGTVE
                     AFLRYPEFGFDYSIRAERQGAGLRITVNLPRALPAELSGKAALNLEFLPSAYFGKAWI
                     MDEQSGLFPLYPTGLKEQEGSTPPALAEGRRFTLAPDDPARRVTIEAKEAGLAVYDGR
                     AKAQNGWFVVRSLIPSGKSGTVLEWTLEANSIDGWLRDPVIG"
BASE COUNT          590 a          945 c          978 g          560 t
ORIGIN      
        1 actaacgccc cacgcagtgt gtagatgtta tgtatcgtgc cggtgatatt ctcggcggcc
       61 cagaacgcct tcatctgcgg gaaccaggtg ttcatttcgg ccggcgcttc ggtgcccggc
      121 gtgttctgga acaacatcgt cacaccgtca acggtaagtg tctcgaacgg cttttcaatg
      181 atgacgttag gctcgatcag tccagtgttt ccgaccgcag tgttcttgcc gatcgattgg
      241 tcgatatggc caaacgggct gcgcggcagc agcacgccgt actgataaaa catgcgccga
      301 gtcattgcgg tgccggccat gacgttctcg gaaaccgcat gatccatgaa tccgacgggc
      361 gcgatgatct tgaccttgcc gctgcgtacg tcggcttctt ccaccacgcc gcggacaccg
      421 ccgaagtgat cgccgtgtga gtgagagtag acaaccgcaa ccaccggccg cttgccgagc
      481 gtgtcattgg caaagtcgag cgccgccttg gccgtttctt tagccgtcaa cggatcgaac
      541 acgatccagc ctgtatcgcc tttcacgagt gtgaaattgg cgaggtcgta gccgcgaact
      601 tgatagatcc gttcgggcag tacctcgtac aggccataag ccatatttaa gacggcttga
      661 cgttgcaacg atggatgaat gctggcgaag ttctgatcgc taagaaggaa ctcgtagctc
      721 gccatgtccc aggccacatg gcccgcctcg gctttgatct gcttgtaagt cggcgctgcg
      781 atgaaacccc tctttgcctc ctcgaaatca cgtcgatcct cgaatggcaa cgtggcacgc
      841 aaaccgtctt gcagttcgat ggtgaacgtc gatggcggct tacccttggg atggaagtgt
      901 tgttcttgcg actgcgctgc gctggtggtg atcgctgcgg acaaaatgag tgcgaggagg
      961 agcggattga gtaagttcat ctcgtgtact cccaaatgag gaagcgcggt ttggcgacaa
     1021 agtctacaac agaaaaaatc gaagtcgaga ggcgcgcgtg ccgatagcaa tatcgcgtag
     1081 cacgagcgca gacctccacc atgccgcccc gccaggcgcc attctcgccg aagtgcactg
     1141 atcgttgacc gtcgacaggc cgcggctggc cgcggctcca cggctacagg cagcgcttct
     1201 cgaactgctc cagcagttcc tcgcggtctg cggcgtcggc gaagaattcc ccgaggtatt
     1261 cgcggcgctt gcggcgggcc tcctcggatg cggccggcaa ttccgcggcg agcgcgagta
     1321 tgtcggcctc gcgcaaagtc actgcatcga tggccgccgc gaggtcgtcg ctcgaaatgc
     1381 agtagccgcg gtaccggcgc gttcgcacgc tcgcgatctt gagcgacgca tcggggcgcg
     1441 cgtagctcgc gtgcacgagg cccgcgaggt cgaagtcgta cggcaccggc agggtgcggc
     1501 cgtcccgctc gtagaggtcg acgttgtggc agcaggcgtc cgcggtctcg gccgtgacca
     1561 gtgaccagtc ggtattcccg atgagatact ggaacacgta aaccagtgca gcctggcggc
     1621 cgtcgagccg cgacagggcg acgccgggcg tatccagcac cgccgccccg cgccgcgcgg
     1681 cgagagcctc gtcgctttcg atcaggaagg catcgcgaac gagcgctgcc agccccgtac
     1741 tgtcggtgtc gacgtatgcc atgcgtagca ggcggacgcg atagccgtcg tcggacacga
     1801 gattgaacag ccggtaggcg agatactcgt cgagcaggtt gcctgcatcg cgcttcctgt
     1861 cattgcagtg ggtaacgagc ttcagcttgt cctgtccgtc gaagacactg ccggcggtac
     1921 ccttgcctgg gaaatcgagc ctgagcggcg gaaagctgca aacccgcagg cggctgttgc
     1981 cgcgaatacg cacttcgacc ggtatgtcga caccgtcgac actgacgatg aacggaaact
     2041 ccatgcgttc agcaccggat tccagcaccg cgtggagcgg gccggcaagc cgcacgtcga
     2101 gcacgggctc ctcgtcgaag agcgcaccgg cccggacggc gccggccgct gccaggaaga
     2161 ccagcgggag aacctggcgc aagcagtagt ttccttttat ttcaaaggct tcttcgagcc
     2221 ggacgccgct ataatggacc cccggccggg aaagcgccat cgccatccgg ggacatcgga
     2281 cgaaggggga tatcttgaag cacgttgcaa gcttattgtg cgcctgcgcg ctcattgcgg
     2341 gcgggcatcc ggcgcgggcc ggcgacccgg ttctgaacga actcgaatac ttccagtcag
     2401 gcagcgtcga ttacctcgtc ttcagcaact ggtacgacgg cctgttcagc gactcgaaga
     2461 tcagcggcat cgagctcatc catcacggcg agcggactgc gaccaacggt gacgtgcggc
     2521 tcgatgccac gccggagcaa tgggaccgca tccccgaatt cgtcgagaag aaggtggacc
     2581 gggagcgcgg cacggtcgag gccttcctgc gctacccgga gttcggtttc gactactcga
     2641 tccgggcgga gcggcaggga gcagggctcc gcatcacggt gaacctgccg cgggcgctgc
     2701 ccgcagaact ctcgggcaag gcagcgctca acctcgagtt tctcccgtcc gcctatttcg
     2761 gcaaggcgtg gatcatggac gagcagtcgg gactctttcc gttgtatccc acgggactca
     2821 aggaacagga aggcagcaca ccgccggcac tcgccgaagg gcgccgtttc acgctggcgc
     2881 ccgacgaccc ggcgcgccgc gtgaccatcg aggcgaagga ggccgggctt gccgtctacg
     2941 acggccgcgc gaaagcgcag aacggctggt tcgtcgtgcg aagcctcatc ccgtcaggaa
     3001 agagcggtac ggtactcgag tggacgctcg aggcgaattc gatcgacggc tggctccgcg
     3061 accccgtcat cgg
//
LOCUS       DASUOM010000126         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_146671,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000126 DASUOM010000000
VERSION     DASUOM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146671"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFY27_02525"
     CDS             complement(<1..285)
                     /locus_tag="VFY27_02525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255678.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5764413.1"
                     /translation="MSILVNKNSKIVCQGITGNQGSFHTSQCIEYGTRVVAGVTPGRG
                     GDEHLGVPVFNTMRGAVAETGADVSMIYVPAAFAADAILEAADSGVALIVC"
     gene            complement(282..1442)
                     /gene="sucC"
                     /locus_tag="VFY27_02530"
     CDS             complement(282..1442)
                     /gene="sucC"
                     /locus_tag="VFY27_02530"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5764414.1"
                     /translation="MNLHEYQSKRLFADYGIPVPRGVPAKTTDEAVKGAEQLGGNLWV
                     VKAQVHAGGRGKAGGVKLARSFDEVRQHAGDMLGKQLVTHQSGPEGMPVNMVYVEAGS
                     EIERELYLSMLIDREASKISFIASAAGGMDIEKVAAETPEKIFTVAVSPAAGLQDFQA
                     RQLAYGLGLDKAQGREFADLIRRLYRLFLEKDASLVEVNPLIVTKSGDVVALDAKINI
                     EDNALFRQKSLAELRDPSQEDATEREAAQHDLNYVSLDGDIACMVNGAGLAMATMDII
                     KLHGGEPANFLDVGGGATSERVTAAFKLILSNEKVNAILVNIFGGIVRCDLIAQGIID
                     AVKGVGVTVPVVVRLEGTNVERGRELLAASGFDIITAGDLTDAAKKVVAAAK"
     gene            1592..3289
                     /locus_tag="VFY27_02535"
     CDS             1592..3289
                     /locus_tag="VFY27_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764415.1"
                     /translation="MKPTTAVMAQATAADPGAPPENTARLRAFDNIELTWRVLGTLNV
                     FRLAVPVVLLLLFVAGSEGRIFGERYPDLFGATAVAYLLFAVISAAAIRQRWLPAVPQ
                     AIAHIIVDIAAVIALMHASGGIASGLGGLLIVFVGAGSLVLPGQLPAFFAAVATLVIL
                     GEQFFSQLAGASDTGNYPAAGVLGAIIFAISLAAKPLARRIQVSEELARQRGLDLANL
                     SELNEYIVQHLRESIVVLDADDRVRLINASAAQLLGAPEACTGTRLESIAPTLAGFIG
                     DWRRDLTRKSHLEFTMIAADNSARITAHLAPLAKDDERGGPLLVFLEDASLMNARVQQ
                     SKLASLGRLSASIAHEIRNPVGAMSHAGQLLAESKALSEDDLRLTEIITTHAERVSHI
                     VDNVLQLSRRESSRPEQFLLKPWLTDFADEFTRTLELQEGELAVGTVPNDLEVRMDPS
                     HLRQVLWNLCDNAVKYASETGGILVELQAGRMQRTGRPYLEVLDHGHGVDPATVDKIF
                     EPFYTDRKGGTGLGLYISRELCELNRATLVYQARENGGSIFRIVFADPSRWGAVENQ"
     gene            3286..4599
                     /locus_tag="VFY27_02540"
     CDS             3286..4599
                     /locus_tag="VFY27_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5764416.1"
                     /translation="MSKPLALVIDDEPDICELLALTLGRMNIETDLSADVTGAKQKLS
                     RRKFDICLTDMRLPDGNGLELVEWMQTHAPGVPVAVITAHGNVETAVQALKLGAFDFV
                     SKPLDLTNLRNIVDSALKLGQKPFDAGRSPLLGNSDVMRHLRDMIDKVARSQAPVHIS
                     GESGTGKELVARLIHDSGPRRDGPFIPVNCGAIPSELMESEFFGHRKGSFTGAVNDKV
                     GLVQCASGGTLFLDEIADLPLSMQVKLLRVIQEQTVRPIGASKEETVNIRILSATHRK
                     LSNLVATSEFREDLYYRINVIELHVPALRERGDDILLLAGRILEKLGSSVAALDEDAK
                     AALLNYSYPGNVRELENLLERAVTLCSDGRIRESDLSLRRSSAPDSDHPPVGATNLGN
                     QIESVQRQAIVEALERARYNKTAAAKLLGLTFRQLRYRIKKLGIE"
BASE COUNT          913 a         1472 c         1506 g          901 t
ORIGIN      
        1 acagacgatg agcgcgactc cggaatccgc ggcttccagg atcgcgtccg ccgcgaacgc
       61 cgccggcacg tagatcatgc tgacgtccgc gccggtctcg gctacggcgc cgcgcatggt
      121 gttgaaaaca ggaacaccta agtgctcatc gccgcctctt cccggagtga cgccggcaac
      181 cacgcgggtg ccgtattcaa tgcactggct ggtatgaaac gatccctggt tgccggtgat
      241 gccctggcag acgatcttgc tgttcttatt gacgagtatg ctcatttcgc tgccgcaacg
      301 actttcttcg ccgcgtccgt caaatcaccg gcggtgatga tgtcaaagcc gctggctgcg
      361 agtagctcgc gccccctctc cacgttcgtc ccttccaacc gcaccaccac tggaacggtg
      421 acgccgactc ccttgaccgc atcgatgatg ccctgggcaa tcaggtcgca gcgaacgatg
      481 ccgccgaaaa tgttgaccag gatggcattt accttctcgt tcgacaatat gagcttgaac
      541 gcagccgtca cgcgttcgct ggtagcgccg ccgccgacgt ccaggaagtt tgcaggctcg
      601 cctccgtgca gcttgatgat gtccatggtt gccatagcga gaccggcccc gtttaccatg
      661 caggcgatgt cgccgtcgag cgaaacgtaa ttcaggtcgt gctgtgctgc ctcgcgctct
      721 gtcgcgtcct cctggctggg gtcgcggagt tccgccaggc tcttctgcct gaacagcgcg
      781 ttgtcctcga tgttgatctt cgcatccagc gccacgacgt cgccgctttt cgtgacgatc
      841 agcggattta cctccacgag gctcgcgtcc ttctcgagga acaaccggta aaggcggcga
      901 atgagatcgg caaactccct gccctgtgct ttgtcgaggc cgaggccgta ggcgagctgt
      961 cgcgcctgga aatcctgcag gcccgccgcc ggcgacacag cgacggtaaa gatcttctcg
     1021 ggtgtctcgg ccgcgacttt ctcgatgtcc atgcctcccg cggccgacgc gatgaaggag
     1081 attttgctgg cttcgcggtc gatgagcatg ctcaggtaaa gctcccgctc gatctccgaa
     1141 ccggcctcga cataaaccat gttgaccggc atgccctccg gtccggactg gtgggttacg
     1201 agctgcttgc ccagcatgtc gccggcatgc tggcgaactt cgtcgaacga acgcgcgagc
     1261 ttgacgccgc ctgccttgcc ccgtccgccg gcatgcacct gcgctttgac aacccagagg
     1321 ttgccgccca gctgttctgc gcccttgacc gcctcgtctg ttgtctttgc aggcacgccc
     1381 ctggggaccg gaatgccata gtcggcgaac agccgcttgg actggtattc gtgcagattc
     1441 atttactgga ttttcttttg ccggtggaga agtcggcgta ttttggagga aagcgcaggc
     1501 agacgcaaag ccatgctccg gctggggttc tgtgggcaat cgcacgacaa tgctacctgc
     1561 cgtgcgtatc attgcgccgg acttgaacat cttgaaacca acgacagcgg tcatggcaca
     1621 ggcaacagcg gcagatccag gggctccgcc ggagaacacc gcccggcttc gcgccttcga
     1681 caacatagag cttacgtggc gcgtgctcgg cacgctgaac gtgtttcggc tggccgttcc
     1741 tgtcgtcctg ctcttgctgt tcgtcgcagg ctccgaaggc cgcattttcg gcgagcgcta
     1801 tcccgacctg tttggcgcca ccgcagttgc gtatctgctg ttcgccgtaa tctccgctgc
     1861 cgcaatccgg cagcgatggc tgcctgccgt accgcaggca attgcgcaca tcatcgtaga
     1921 cattgctgcc gtaattgcgc tgatgcatgc aagcggcggc atcgccagcg ggctcggcgg
     1981 actgctcatc gtgttcgtgg gggcaggcag cctggtgctg ccgggacagc tgccggcgtt
     2041 ctttgcggcc gtcgcgacgc tcgtgatcct gggcgagcag ttcttttcgc aactcgccgg
     2101 agccagcgac accggcaatt acccggccgc aggcgtcctg ggggcgatca tctttgcgat
     2161 ctctttggcg gcaaagccgc tggcgcgacg catacaggtg agtgaagagc tggcgaggca
     2221 gcgcggcctg gacctcgcca acctttcgga attgaacgag tatatcgtgc agcacctgcg
     2281 ggaaagcatc gtagtgctgg acgcggatga ccgggtgcgc ctgatcaacg cttccgcggc
     2341 acagctactg ggggcgcctg aagcttgcac ggggacacgc ctggaatcga ttgcccccac
     2401 cctggccggc ttcatcggag actggcggcg ggacctgaca cggaagtcgc atctggaatt
     2461 cacgatgatc gccgcggaca acagcgcccg cataactgcg caccttgccc cgctcgcaaa
     2521 agacgacgag cgtggcggtc cgctgctcgt gtttctcgag gacgcgagcc tgatgaacgc
     2581 gcgtgtgcag cagtcgaaac tggcctccct cggacgcctg agcgcgagca ttgcccacga
     2641 gatccggaat cccgtcggcg cgatgagtca tgcaggacag ctgctggccg agtcaaaggc
     2701 actttccgaa gacgacctgc gcctgacgga gatcatcacg acgcacgcgg aacgggtgag
     2761 ccatatagtg gacaacgtgc tgcagctgtc cagaagggaa tccagccggc cagagcagtt
     2821 tttgctgaag ccttggctca cggacttcgc cgacgagttc acccgcacgc tggaactgca
     2881 ggagggggag cttgcagtgg gcacggtgcc gaacgacctg gaggtgagga tggacccgag
     2941 ccacctgcgg caagtgctgt ggaacctgtg cgacaacgcg gtgaagtacg cgagcgaaac
     3001 cggcggcatc ctggtcgagc tgcaggcggg acgaatgcag cgcaccggca ggccgtatct
     3061 ggaagttctt gaccacggtc acggagttga cccggcaacg gtggataaaa tcttcgagcc
     3121 gttttacacc gaccgcaaag gcgggaccgg gcttggcctt tatatctcac gcgaactgtg
     3181 cgagctcaat cgagccacgc tcgtgtacca ggcgagagaa aacggaggca gtatcttcag
     3241 gatcgtattt gcagacccga gccgctgggg agccgtggaa aatcaatgag taagccgctg
     3301 gcactggtca tagacgacga gcccgacata tgcgagttgt tggcgctcac gctcggccgc
     3361 atgaacatcg agacggacct gtccgcggac gtcaccggcg cgaagcagaa gctcagccgg
     3421 cgcaagttcg acatctgcct cacagacatg cggctgccgg acggcaacgg gctggaactg
     3481 gtggagtgga tgcagacgca cgccccgggc gtgccggtgg cggtcatcac cgcccacggc
     3541 aacgtggaaa cggcggtcca ggcgctgaaa ctaggggcct tcgacttcgt ctcgaagccg
     3601 ctcgacctga cgaacctgcg caacattgtc gacagtgccc tcaagctggg gcagaagcct
     3661 ttcgacgccg gccgctcccc gctgctcggc aactcggacg tgatgcggca cctgcgcgac
     3721 atgatcgaca aggtagcgcg cagccaggcc ccggtgcaca tctccggcga atccggcacc
     3781 ggcaaggagc tggtggcgcg cctgattcac gacagcggcc cgcggcgcga tggcccgttc
     3841 atcccggtga actgcggggc gattccctcc gagctgatgg agagcgagtt cttcgggcac
     3901 cgcaaggggt cgttcacggg tgcggtgaac gacaaggtcg ggctggtgca atgcgcaagc
     3961 ggcggtaccc tgttcctgga cgagattgca gatttgcccc tgtccatgca ggtgaagctc
     4021 ttgcgggtga tccaggagca aacggtccgg ccaattggcg cttcgaagga ggaaacggtg
     4081 aacatccgga tcctctcggc gacgcaccgg aagctttcga acctcgtcgc aacgtccgag
     4141 tttcgcgagg acctctatta ccgcataaac gtgatcgagc tgcacgtgcc ggcgctgcgt
     4201 gagcgtggcg atgacatttt gctgctggcg ggcaggatcc tggaaaagct cgggtcgtcg
     4261 gttgccgcac tggacgagga tgccaaagct gccctgctga actactcgta cccgggcaac
     4321 gtgcgggaac tggagaatct gctcgagcgc gcggtgacgc tttgctcgga tggacgcatt
     4381 cgcgagagtg atttgagcct gcgcaggtcc tccgctccgg atagcgacca cccgcccgtc
     4441 ggagcaacga acctgggcaa ccagatagag agcgtgcaac gacaggcgat cgtggaagcg
     4501 ctggaaaggg cgagatacaa caagacggct gcggccaaat tactaggctt gacgttccgg
     4561 cagttgcgct atcggattaa gaagctaggt atcgagtagc tccccggtat tgcaaatgct
     4621 tctcattctg gaaacacctc cgcggaggac ttcgtcgagc gtcgaaccag cagataggct
     4681 ggtatagcca gcatggtggt tgtcccaacg actaagagtc gatcaatcag cgctacgaca
     4741 gcgcctgatt ccggggagat gtccagcaaa atggcggccc cgattaccgc cc
//
LOCUS       DASUOM010000127         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1066630,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000127 DASUOM010000000
VERSION     DASUOM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066630"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1164
                     /locus_tag="VFY27_02545"
     CDS             91..1164
                     /locus_tag="VFY27_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX5764417.1"
                     /translation="MTFQDGAPAVSGTPRRLELPQPYLLFLGDIIEPDFAKTAFGLRD
                     WAEDRCVGEMSLGLHAVTTGLPGLTPAEAKARGARAMVIGVANVGGELKDTWIASLVA
                     GLEAGLDIVSGMHMRLALTPELKSTAERLGRRLIDVRTPPEGIPIATGSKRSGKRLLT
                     VGTDCALGKKYTALSITGALKARGIDADFRATGQTGIMIAGSGIPMDAVVSDFAAGAA
                     EILTPDAAADHWDVIEGQGSLFHPSYAGVSLALLHGSQPDVIVVCHQPGRMKIVGTAG
                     YEVPGIPETIDLNLRLGSRTNPAIRCAGVTLNTSRLPERAARQLMEDEAARLQLPVAD
                     PIRGGAEFERLVDSCLESAGREL"
     gene            1164..1556
                     /gene="crcB"
                     /locus_tag="VFY27_02550"
     CDS             1164..1556
                     /gene="crcB"
                     /locus_tag="VFY27_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5764418.1"
                     /translation="MPGLSGVLLVGLGGFAGSIARYKLGGYVLHLTVQERFPFSTLAV
                     NVLGCLLVGLLAGLAERFDLFGPETRLFLFTGVLGGFTTFSAFGLDAVFLLRRGEIWM
                     AALYAGLSVVLGVAAVCFGLKLVSLLPR"
     gene            1683..2324
                     /locus_tag="VFY27_02555"
     CDS             1683..2324
                     /locus_tag="VFY27_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5764419.1"
                     /translation="MSTRAETVAKQHADNARYRQQRQEAIKAAAAVFAEKGFHGASTR
                     DIAECLGIKQGSLYYYFRSKEEALGEVCLFGIEDYVRRMERIAKSDQPFEAKLLATLT
                     SHLSSYREKNEALKVYNDERLYLPEEKRKVLKQLGSGYRQLLEGIFEEGIRQGVIRNS
                     IDCHFAAQAVIGICNAWGDLIVRDPDLDIFDVTQKSADLLLNGFSERRKNKRD"
BASE COUNT          475 a          720 c          769 g          453 t
ORIGIN      
        1 cgtaggagcg gcctgcgcac gcgcacaact ggatccggaa agagatgagc gagcgaaccg
       61 gaaactattg aggcacaagg acgtactgcg atgactttcc aggatggtgc tccggccgtt
      121 tcaggcacac cgcgccggct cgagttgccg cagccttacc tcttgttcct cggcgatatc
      181 atcgagccgg attttgccaa gacagcgttt ggcctgcgtg actgggcgga ggaccgctgc
      241 gtcggcgaga tgtccttagg cttgcacgcg gtcacgacgg gactgccggg actgacgccg
      301 gcggaggcga aggcaagggg tgcgcgcgcc atggtgatcg gcgtggcgaa tgtcggcggc
      361 gaactcaagg acacctggat cgcatctctc gttgccggac tcgaagcggg tctggatatc
      421 gtgagcggca tgcacatgcg ccttgcgctc actcctgaac tgaagagcac cgcggaacgc
      481 cttggacgca gattgatcga cgtgcgcacg ccgccggagg gaatcccgat tgccaccggg
      541 agcaaaagga gcggcaagcg cctgctcacg gtcggcacgg attgcgcgct gggcaagaag
      601 tatacggcgc tgtccataac gggcgcgctc aaggctcgcg gcatcgatgc cgacttccgc
      661 gcgaccggcc agaccgggat catgatcgcc gggagcggca tcccgatgga cgcggtcgta
      721 tcggacttcg ccgccggcgc ggccgagata ctgaccccgg acgcggcggc cgatcactgg
      781 gacgtgatcg aagggcaggg ctcgttgttt catccgtcct acgcgggcgt ctcgcttgcc
      841 ctcctgcacg gtagccagcc cgacgtcatc gttgtctgcc accagccggg gcgcatgaag
      901 atcgtgggaa ccgcaggata cgaagtgccg ggcatcccgg agacgatcga cctcaacctg
      961 cggctcggca gccgcacgaa tccggcgata cgctgtgccg gtgtgaccct caacacctcc
     1021 cgcttgccgg agcgggccgc acggcaactg atggaagatg aggccgcgcg cctgcagctg
     1081 ccggtggcgg atccgatacg cggcggcgcc gagttcgagc gcctggtcga cagctgcctc
     1141 gaatccgcag gtcgtgaact ttaatgcccg gcctgagcgg tgtcctgctt gtcggtctcg
     1201 gagggtttgc cggttcgatc gcacgctaca agcttggcgg atacgtcctg catttgaccg
     1261 tacaggagcg ctttccattc agcaccctgg cggtcaacgt tctcggttgc ctgctcgtcg
     1321 gtcttctcgc agggctcgca gagcgattcg atctgttcgg gcccgaaacc aggctgtttc
     1381 tcttcaccgg cgtgctgggt ggcttcacca ctttctcggc ttttgggctg gacgcggttt
     1441 tcctcctgcg gcgaggggaa atatggatgg ctgcgttgta cgccggcttg agtgtcgttc
     1501 ttggcgtcgc cgccgtctgc ttcggcttga agctggtctc gctactccca cggtaggcct
     1561 tctgcgctct aagcagccgt catcaataga atgactggtt atgtaacgtg gtaactggtt
     1621 gtttattcgc gcattctaga tttcccttga caggattcta tcgatctata gaattccgcg
     1681 tcatgagcac acgggccgaa acggtggcga agcagcacgc ggacaacgcg cgttatcgtc
     1741 agcagcgcca ggaagcgatc aaggctgccg ccgccgtatt cgccgagaaa ggatttcacg
     1801 gcgccagcac cagggacatc gccgagtgct tagggatcaa gcagggcagc ctctattact
     1861 actttcgctc caaggaagag gcactgggcg aagtctgcct gttcgggatc gaagattacg
     1921 tgcgtcgaat ggaacgcatc gcgaagagcg accagccatt cgaagcgaag ctgctggcaa
     1981 cgctgacctc gcacctgtcg agctatcggg agaagaacga ggcgctcaag gtatacaacg
     2041 atgagcgcct gtacctgccg gaagagaagc gcaaggtgct gaagcaactg gggagcggat
     2101 atcgccagtt gctcgaagga atattcgaag agggcattcg gcagggagtg atccgcaact
     2161 ccatcgactg ccatttcgct gcacaggccg tcatcggcat ctgcaacgcc tggggagacc
     2221 tcatcgtacg cgaccccgac ctcgacattt tcgacgtcac ccagaaaagc gccgacctgc
     2281 tgctgaacgg attttccgaa cgccgcaaga acaaacgcga ctgacgacgt cgttctcgcc
     2341 accgatcact gaaacctacg gagggattta tggccgaaca gaaagcaaca aacgtaacct
     2401 ggcacagcgg cgacgtg
//
LOCUS       DASUOM010000128         5404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1599606,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000128 DASUOM010000000
VERSION     DASUOM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599606"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFY27_02560"
     CDS             complement(<1..1496)
                     /locus_tag="VFY27_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5764420.1"
                     /translation="MDKVADSRYPPADDGADLPWHARKVDEVLDAVRSTRGGLSEEEA
                     ARRLQDHGPNELPETSGRHPLIRFLSQFHNALIYFLLAAVVATLLLRHYVDAAVILAV
                     VLANAIVGYVQEGKAERALHAIRNLIAPHAHLVRAGRRELLPVRNIVPGDVVLLEAGN
                     RVPADLRLIRARSFLVEEAILTGESVAAEKHVAPAGVAAPLGDRHCMAYSGTFVAAGH
                     AAGVAVGTGTDTEIGRISTLLGSVETLTTPLLRQINRFGTRFTWIALAVAAALFVFAV
                     LLRGYAWQEALVVVVALAVGAVPEGLPAVITITLAIGVQRMAQRNAAIRRLPAVETLG
                     ATTVICSDKTGTLTYNQMMAQRVELAAGRIDVTGSGYSPEGALEPATHLEGETLLTAA
                     IPLLRAAVLCNSSELRESDGHWKVSGDPMEGALLALAMKAGLDPGELREEWSRIDAIP
                     FDARHRFMATLNRSGDGESHIFVKGAPERVLQMCRTQGGNESLEPLAI"
     gene            complement(1543..4221)
                     /locus_tag="VFY27_02565"
     CDS             complement(1543..4221)
                     /locus_tag="VFY27_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX5764421.1"
                     /translation="MSIRNLDALFTPSSIALIGASNQPRSVGSVLAMNLFEGGFSGPI
                     VTVNPRERAIRSSLNYRSVSDLPLNPDLAVISTPPGPVPGLVAELAGRGCRAAIVITA
                     GFGEGDQAEGQALMQEILDAAKPSLLRVVGPNCLGVLAPRSGINASFAHLAALQGDIA
                     FVTQSGAVATSVLDWASSRGIGFSKVVSLGSMSDVDFGDMLDYLALDRDTTSILLYIE
                     AVTHARKFLTAARMASRTKPVFVVKAGRSAAGARAALSHTGALAGSDAVYDAAFRRAG
                     MLRVRTLDELFHAVGTLATGVRVNGDRLAILTNGGGIGVLAVDHLADERGTLAELSEN
                     TLARLDALLPGPSSRGNPVDILGDAQGDRYAGALEALLGDPGADAILVMNCPVAVADS
                     LECARAVVDTVGSRPHAPVLTCWLGEGTAAEARRLFASRRIPSYDTPEQAIQAFRHLG
                     RYRRNQQLLMETPPDVSDLISIDCTKANAVIDGVLGDGRTVLTEPEATAILAAYGIPT
                     VASTVARDPEAAGRAAEELGFPVVLKILSRDISHKSDVGGVRLNLASGVAVARAAEEM
                     LASVSQAAADARLDGFNVQRMIRRRDAHELIAGVSEDQVFGPVILFGEGGTAVEVIGD
                     RSVGLPPLNPLLADEMIRATRISRLLRGYRDRPAADLEAVSLTLVKLSHLVTTLGRVV
                     ELDINPLLADKSGVVALDARIVVRRGGREERPLAIRPYPQQLEREIETRSGLRLKVRP
                     VRPEDEAAMVDMLRRSSKEDVRMRFFGTLKDFDHPFAARLTQIDYDREMAFVALKPDS
                     SEIVGAVRLSADPDREHAEYAIMVRSDMKGSGLGYALMSQILDYARATGISRVFGDVL
                     RDNDRMLRMAREFGFAVKPAEAGSDAVIVELLLDKR"
     gene            4656..5342
                     /locus_tag="VFY27_02570"
     CDS             4656..5342
                     /locus_tag="VFY27_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764422.1"
                     /translation="MLRYLSRRHKTLMDESEQALYAAAQQDSGADARIAKQVISDTKT
                     HGLWEARHAELVRPVAEQNSRAPQVIALRDLEVRLVHRRALFDHIREHQLRGKDRERL
                     FQVFYGPREFQDAVLAEHRQYMLAVSSRVSADHLIDVMSEPRSKKLLEDYEAAYACYF
                     DLYCYVVGAEDRTCAEATRHLMTLARERAERLRQRISTERPDNRAADFERQAILARSG
                     RYPVLDYMVG"
BASE COUNT         1035 a         1707 c         1716 g          946 t
ORIGIN      
        1 tcgatagcga gcggctccag gctttcgttg ccgccctgtg tccggcacat ctgaaggact
       61 cgctcgggcg cgcctttgac gaatatgtga gattcaccat cgcccgagcg gttcagggtc
      121 gccatgaagc ggtggcgcgc gtcgaacgga atagcatcaa ttcgcgacca ttcctcccgc
      181 agctcaccgg ggtccaatcc tgccttcatc gcgagcgcga gaagggcccc ctccatcggg
      241 tctcccgaca ccttccagtg accatccgat tcccggagct cggagctgtt gcatagaaca
      301 gcggcgcgga ggagcgggat ggcggcggtc aataatgtct cgccttccaa atgcgtcgcg
      361 ggttccagcg ccccctcggg cgaatagccg gagcccgtca catcgattct ccccgcggcg
      421 agttccaccc gctgcgccat catctggttg tacgtcagcg tgccggtctt gtcggagcag
      481 atgacggtgg tcgcgccgag cgtctcaact gccggcagtc gccggattgc ggcgttccgc
      541 tgcgccatcc tttgaacgcc gatggcaagc gtgatcgtaa taacggccgg tagtccctcc
      601 gggaccgcac cgactgccag cgcaaccacg acaaccaggg cttcctgcca cgcatatccg
      661 cgcaacagga ctgcaaatac gaacagcgcg gctgcgacag caagtgctat ccatgtgaag
      721 cgcgttccaa agcgattgat ctgccgcagc aacggcgtcg tcagcgtctc tacgcttccg
      781 agcagcgtgc tgatacggcc gatttcggtg tccgttccgg ttccaaccgc gactccggca
      841 gcatggcccg cggctacaaa ggtaccggag taggccatgc agtggcgatc gcccaaggga
      901 gcggccacgc cagcgggggc aacgtgcttc tccgcagcga cggactcgcc ggtcaggatc
      961 gcttcctcga ccaggaacga ccgggcccga atcagccgca agtctgcggg cacgcgattt
     1021 ccagcctcca gcaacaccac atcgcccggc acgatgttcc gcaccggcaa tagctcgcgc
     1081 cgcccggctc gcacgaggtg cgcgtgcggg gctatcaggt tgcggatggc gtgaagggct
     1141 ctttcggctt tcccttcctg cacatagcca acgatggcgt tagcgagcac gaccgccagg
     1201 atcacggcgg catcgacgta gtgacgaaga agcagcgtcg cgaccacggc tgcaagcagg
     1261 aagtagatca gcgcgttgtg gaactgcgag aggaagcgga tgagcggatg ccgtccggaa
     1321 gtctcgggca gttcgttcgg cccgtgatcc tgcaagcgcc gcgcagcctc ctcttctgac
     1381 agtccgccgc gagtgctgcg gacggcatca agcacctcgt cgaccttccg ggcgtgccag
     1441 ggcaagtccg cgccatcgtc agcgggcggg tatctgctgt cggcaacctt gtccactgga
     1501 tattccgtgt cggctctcgg aggcggaagc gatgcggcgc gcttagcgct tgtcgagaag
     1561 cagttcaacg atcactgcgt ccgaaccggc ctctgccggt ttgactgcga agccgaactc
     1621 gcgcgccatc cgcagcatgc ggtcgttgtc cctgagcaca tcgccgaaca cacggctgat
     1681 tcccgtcgcg cgcgcgtaat cgagtatctg cgacatcagg gcgtaaccga ggccgctgcc
     1741 tttcatgtcg ctgcgcacca ttattgcgta ctctgcatgt tcacggtcgg gatctgcgct
     1801 cagccgtacc gcgccgacta tctcggagga atccggcttc agtgccacga aagccatctc
     1861 gcggtcgtag tcgatctgcg tcagccgggc ggcaaaagga tggtcgaaat ctttgagcgt
     1921 gccgaagaag cgcatgcgta cgtcttcctt cgaagagcgg cgcagcatat cgaccatcgc
     1981 agcttcatcc tccggcctca cgggacgcac cttgagccgc agcccgctgc gcgtttcgat
     2041 ctcgcgctcc agctgctgcg ggtacggccg tatagcgaga ggccgctcct cacggccgcc
     2101 ccgccggacg acgatgcgag cgtcgagcgc cacgacgccg gacttgtcgg ccaacagcgg
     2161 gttgatgtcc agttccacca ctcgcccaag cgtagtgacg aggtgtgaaa gtttgaccag
     2221 cgtcaggctc acggcctcca ggtcggcagc agggcggtcg cgatacccgc gcagcaggcg
     2281 cgagatgcgc gtggcgcgga tcatctcgtc ggcgagcagc gggttcaggg gaggcaggcc
     2341 tacggaacga tccccgatga cctcgacggc cgtgccgcct tcaccgaaca ggattaccgg
     2401 cccgaaaacc tggtcctcgc tcacgccggc gatcagttcg tgcgcatcgc ggcgccggat
     2461 catgcgttgc acgttgaatc cgtctagtcg tgcatccgct gcagcctgac tgacggaggc
     2521 gagcatctcc tcggctgctc gcgcaacagc gacaccggag gcgaggttca gacgtacgcc
     2581 acccacatcg gacttgtgtg agatgtcgcg cgacaggatc ttcaacacca ccggaaagcc
     2641 cagctcttcc gccgccctgc ctgcggcttc gggatcacgg gcaacggtgg atgctactgt
     2701 tggtatgcca taagccgcca ggatcgccgt tgcttccggc tccgtcagta ccgttcgacc
     2761 gtcaccgagc accccgtcta tgacggcatt cgctttggtg caatcgatgg atatcaggtc
     2821 cgacacgtcg ggtggcgtct ccatcaggag ttgctggttg cggcggtaac gtcccaggtg
     2881 acgaaaagcc tggatagcct gctcaggcgt gtcgtatgaa ggaatcctgc gactggcaaa
     2941 caggcgcctg gcctcagcgg cggtaccttc gccgagccag cacgtgagca ccggcgcatg
     3001 cggtcgcgaa ccgacggtgt cgaccacggc gcgtgcacat tcgaggctgt ccgcgacggc
     3061 gaccgggcag ttcatgacga ggatggcgtc ggcgcccggg tcccccagca gagcttcgag
     3121 tgctccggcg tagcggtcgc cttgggcatc tccgaggatg tcgaccgggt tgccgcgcga
     3181 cgaggggccc ggcagcagcg catcgagacg tgcaagggta ttctctgaga gttcggccag
     3241 cgtccccctc tcatccgcca ggtgatcgac agccagtaca ccgatgcctc ccccattggt
     3301 cagtatggcc aacctgtcgc cgtttactcg cacaccggtc gcaagcgtcc cgacagcgtg
     3361 aaacaactca tcgagcgtac ggacgcgcaa catcccggcg cgccggaacg cggcgtcgta
     3421 caccgcgtcc gagccggcaa gagcgccggt gtgggacagg gcggcgcgcg cgccggcggc
     3481 gctgcggcct gccttgacga cgaaaaccgg tttcgtgcgg gacgccatgc gtgcggcggt
     3541 gaggaacttc cgcgcatgcg tgaccgcctc gatgtagagc aggatcgaag tcgtatcgcg
     3601 gtcgagtgcc aggtagtcga gcatgtcgcc gaaatcaacg tcgctcatcg accccagcga
     3661 aaccaccttc gaaaagccga tgccgcgtga ggacgcccag tcgaggacgg aggtcgccac
     3721 ggcacccgac tgggtgacga aagcaatgtc cccctgcaac gccgcgaggt gggcgaagct
     3781 cgcattgata ccgctccgcg gtgccaggac accaaggcag ttgggtccga caacgcgcag
     3841 caagctgggc tttgcagcgt caaggatctc ctgcatcaaa gcctgtccct cggcctggtc
     3901 tccctcgccg aacccggcgg taatcacaat cgcggcgcgg cagccgcggc cggcaagttc
     3961 ggcaacgagc cccggcaccg gccccggcgg ggtactgatg acagcgaggt caggattgag
     4021 cggcaggtcg ctgacggagc ggtagttgag cgaggagcgg attgctcgct cgcggggatt
     4081 caccgtgacg atcggtccac tgaatccgcc ttcgaaaagg ttcattgcga gtacggaacc
     4141 gaccgagcgg ggctggttgc tcgcgccgat cagcgcgata gaggagggcg tgaacagcgc
     4201 atcgaggttt cggatcgaca aggggagtct ccggcgagcg acgggtcggc ccatttataa
     4261 caaacggcca gccatccggc aggcgattat gtaatcctgg aatgcgctgc cgttgccatg
     4321 gccgccagcc ggagctgccg ttaattcgcc ggaagttgcg caaaagtgct ggtgcggtgg
     4381 gcgaggctct tcaggtcgca aagccggcct ccgtcaagtc catttttgcg gcggttaacg
     4441 tgctttctcg aaagccgaag taagcggcga taactgctcg ataatgagat gtcgatgacc
     4501 cacaaaggtc gctgttgcgc ggaaaaaacg ctttgcggcg ccatccggat gcaaacgttc
     4561 acggaaactt catttgaaga aatgactgaa tccgcagtac cttatgtcat aagcaacggc
     4621 agttccccga ctgcaaggaa catgagaagg taaagatgct gcgatatttg tcgcgtcggc
     4681 acaaaaccct catggacgag tccgaacaag cgctgtacgc ggcggcgcag caagattccg
     4741 gagctgacgc aaggattgcg aagcaagtca tcagcgacac gaaaacccat gggctgtggg
     4801 aagcccggca cgcggaactg gtgcgcccgg ttgccgagca gaacagcagg gcgccacagg
     4861 tcatcgcgct gcgggacctc gaagtgcgtc tcgtgcaccg tcgggcgctt ttcgatcata
     4921 tccgggaaca tcagcttcgc ggcaaggatc gggaacggct gttccaggtc ttttatggtc
     4981 ctcgtgaatt ccaggatgcg gtgctggccg agcatcgcca gtacatgctg gcagtttcga
     5041 gccgggtttc ggctgaccac ctgattgacg tcatgagcga accgaggagc aagaagctgc
     5101 tcgaagatta cgaagcagct tatgcttgct atttcgatct ctactgttac gtggtcggtg
     5161 cggaagaccg gacctgcgcc gaggctacca ggcacctcat gacgctcgcg cgcgagcggg
     5221 cggaacggct acgccaacgc atcagcaccg agcgtcccga taaccgcgct gcagatttcg
     5281 aacggcaggc catactagca agaagcggcc gttaccccgt gctggattac atggtcggtt
     5341 aggcgagccg ccgcgaagag ggggcattgc gcccccgcag gtgtttccct ataatcccgc
     5401 gatt
//
LOCUS       DASUOM010000129         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_756371,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000129 DASUOM010000000
VERSION     DASUOM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756371"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFY27_02575"
     CDS             complement(<1..846)
                     /locus_tag="VFY27_02575"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5764423.1"
                     /translation="MAQKHAPVNPCSDEGAHEGPRGYAHSLKDGATFVVLDEHGDINR
                     EGLGHQGLYHGDTRHVSRLALSVENAPPVLLYAATRHDNSAYVSHLLSPEFVAGGDTI
                     DAGAIHLERTVTVSAARYYEQLHLTSYALEPVLVRVSYEVDADFRDMFEIRGIERPAR
                     GDASAAEWNRGDISWDYTGRDGIKRGTRVRFDPAPDNCGGNRAEFLLRLEPRQVVTLN
                     LVADCGEASAGAMEASHCSAAAGQVATERRRAAAARCDVRTSNELYNRWLERSAADLD
                     MLSKPV"
     gene            complement(876..1940)
                     /locus_tag="VFY27_02580"
     CDS             complement(876..1940)
                     /locus_tag="VFY27_02580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5764424.1"
                     /translation="MRKILFVDPVATKQYDERDLQSDALGGTEATVVRIAEGLALDHL
                     VVVAQSTREWCRTGPGGVVYAPYRHKYESLGNADVDAVVVVRAHKVLPRLRRRFPDAA
                     MYLWMHCNPGRRLRTLARTCVETNTMVVAVSDYHLDAMTGFYARHDPAAAKHLKATRI
                     YNPVAPGLTPDGTPWDPNKLLFLSSPHKGLEQVLDVFGMLKRRCSDFRLCVANPGYLN
                     WRVPRTDGVIMLGKLRHDQVVSHLRDSLCLFYPQTMFEETFGLVFAEANALGTPVIAH
                     AHGAAPEILDDAAQLCDGADMAEVCDRMLQWREGARPLPRPVERFALPRVLAEWERLL
                     QAETNAMYRPQVPAHASARA"
     gene            complement(1946..2941)
                     /locus_tag="VFY27_02585"
     CDS             complement(1946..2941)
                     /locus_tag="VFY27_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5764425.1"
                     /translation="MEPLLVTVVIPTYNRANGCLARAIDGALAQSYADVSVTVVDDGS
                     TDNTASVVRGYASEPRFSCIRLGRNLGTAQAKNLGVLCCRYDAITFHDSDDSPHADKV
                     LLQARALGLQGHVADSILNWEPFGYSAGQVLSVDIAVGGYNLIKMDGSVHYINKRVSL
                     LDDFFPQLQFPSKTEGDWCLVNCGLFRRSVFERLGGFLDSIEEDRELRNRTIAAGCIY
                     YFIDQPLLTKIEMGDSLTMQDMTGYRGHVRHRDREEVWRRVAMYQGGRVGPYVASDTG
                     VCIDLSSAIVEEVINPENLAFQESVSATPGTYAALCGMFNSEPAQQKLANRAITG"
     gene            complement(2917..>3120)
                     /locus_tag="VFY27_02590"
     CDS             complement(2917..>3120)
                     /locus_tag="VFY27_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764426.1"
                     /translation="FTAALPPLVVSALLLVAGLMFAFQGLAVAALGAFAGALAFAAVL
                     ELATQLNVLRTAGCTRWSRFLSL"
BASE COUNT          576 a          999 c          951 g          594 t
ORIGIN      
        1 gaccggctta gacaacatat cgagatcggc agcggagcgt tccagccaac ggttgtacag
       61 ctcattggag gtgcggacgt cgcagcgtgc ggcggcggct cgccggcgct cggtggcgac
      121 ttgtcccgca gctgcactgc aatgggaggc ttccatggca cctgcactgg cctcgccaca
      181 gtcggcgacc aggttcagcg tgacaacctg gcgaggctcc agtcgcagca ggaactctgc
      241 gcggtttccc ccgcagttgt cgggggcagg atcgaaacgg accctggtgc ctcgcttgat
      301 cccgtcgcgg ccggtgtaat cccacgagat gtcgccgcgg ttccattccg ctgcactcgc
      361 gtcgcctcgt gcgggccgtt cgatgccgcg tatttcgaac atgtccctga agtcggcgtc
      421 gacttcgtag ctgaccctga ccaggaccgg ttcgagtgcg taactcgtga ggtgcagctg
      481 ttcataataa cgggcagccg ataccgttac cgtccgctcg aggtgaattg ccccggcatc
      541 gatggtatcg ccgccggcaa cgaactcggg gctgagcaga tgacttacgt aggcgctgtt
      601 atcgtgacgc gttgccgcat aaagcaggac cggcggcgca ttttctaccg acagcgcgag
      661 gcgcgaaacg tgccgagtgt ctccgtggta caggccctgg tggcccaatc cctcccggtt
      721 gatatcgcca tgctcatcga gcaccacgaa ggtggcgccg tctttcaacg aatgcgcata
      781 cccgcgaggg ccctcatgag cgccttcatc gctgcagggg ttgacgggcg catgcttctg
      841 cgccacaaac gtctcttttg ccgcctgcgg ccggttcacg cccgtgccga tgcgtgagcg
      901 gggacctgcg ggcggtacat ggcgttcgtt tccgcctgca gcagccgctc ccactccgcc
      961 agtacccgcg gcagggcgaa ccgctcgacc ggtctcggca gcgggcgcgc tccttcccgc
     1021 cactgcaaca tgcggtcgca cacctcggcc atgtctgctc cgtcgcataa ctgtgccgca
     1081 tcgtccagta tctccggagc tgccccgtga gcatgcgcga tcaccggggt gccgagcgcg
     1141 ttggcttcgg cgaacacaag gccgaacgtc tcctcgaaca ttgtctgcgg atagaacagg
     1201 cacagcgaat ctctcaaatg gctcaccacc tggtcgtggc gcagctttcc gagcatgatg
     1261 actccgtccg tgcgcgggac ccgccagttg agatagcctg gattggcgac acacaggcga
     1321 aagtcgctgc agcgccgttt cagcatgccg aaaacgtcca ggacctgttc cagccccttg
     1381 tgcggcgaac tcaggaacaa gagcttgttc gggtcccacg gtgtgccgtc gggagtaagg
     1441 ccgggcgcga caggattgta gattctcgtc gccttcagat gcttcgcggc cgccgggtcg
     1501 tgccgtgcat agaacccggt catcgcgtcg agatggtagt ccgataccgc gaccaccata
     1561 gtgttcgttt cgacacaggt gcgcgcgaga gtgcgcaacc ggcgccccgg attgcaatgc
     1621 atccagagat acattgcagc gtcggggaag cggcgtctca ggcgcggcag caccttgtgc
     1681 gcacgcacca ccacgacagc gtccacgtca gcattgccga gcgattcgta cttgtgccgg
     1741 tacggcgcat agacgacgcc gccgggcccg gtgcggcacc actcccgggt gctttgcgcg
     1801 acgaccacca ggtgatcgag ggcaagccct tccgcgatcc tgacgaccgt cgcttcggtg
     1861 ccgcccagtg catcgctctg caggtctcgt tcgtcgtatt gcttcgtggc caccgggtcc
     1921 acgaatagta tttttctcat tgcactcagc ctgtaatggc gcggttggcg agtttctgct
     1981 gggcaggctc gctgttgaac atcccgcaca aagcggcgta ggtcccgggc gtcgcggaga
     2041 cggactcctg gaaagcgaga ttttcgggat tgatgacttc ttcgacgatg gcagacgaga
     2101 ggtcgatgca taccccggtg tcactggcga cgtaaggtcc gactcggccg ccctggtaca
     2161 ttgccacgcg cctccacacc tcttcgcggt cccgatgacg aacgtggccg cggtaaccgg
     2221 tcatatcctg catcgtcagc gaatctccca tttcgatctt ggtcaacaat ggctggtcga
     2281 tgaagtaata aatgcatccg gcggctatcg tcctgtttcg caattcgcga tcctcctcga
     2341 tcgaatccag gaacccgcca agcctttcga aaacactgcg acggaacagt ccacagttca
     2401 cgaggcacca gtctccctcg gtcttcgacg gaaactgaag ctgcggaaag aaatcgtcga
     2461 gcagggaaac ccgcttgttg atgtagtgga cgctgccgtc catcttgatc aggttgtagc
     2521 cgccgaccgc gatgtcgacc gacagcacct ggccggcgct gtagccgaat ggttcccagt
     2581 tcaggatact gtccgcgaca tgtccctgca ggccgagggc gcgcgcctgt aacaggacct
     2641 tgtccgcgtg tggtgaatcg tctgaatcgt gaaacgtgat tgcatcgtac cggcagcaga
     2701 gcacacccag gttcttcgcc tgtgcggtac cgaggtttcg gcccagccgg atgcaggaga
     2761 aacgcggctc cgacgcataa cctcgtacca ccgatgccgt gttgtctgtc gagccatcat
     2821 cgacgacggt gacgctcaca tcggcgtagc tctgcgcgag cgcaccgtcg atcgccctgg
     2881 cgagacagcc gttggcgcgg ttgtaagtag gaatcactac agtgacaaga agcggctcca
     2941 cctagtgcac cccgctgtcc tcaaaacatt caactgcgtg gccagttcga ggaccgcggc
     3001 gaaggccagt gcgccggcaa aggcgcccag tgccgctacc gccagtccct gaaacgcaaa
     3061 catcagccct gcgacgagca gcaaagcgct gaccaccagc ggtggcagcg ccgccgtgaa
//
LOCUS       DASUOM010000130         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1414641,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000130 DASUOM010000000
VERSION     DASUOM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414641"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..2097
                     /locus_tag="VFY27_02595"
     CDS             367..2097
                     /locus_tag="VFY27_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764427.1"
                     /translation="MCATALVSCGGGGGGGGPAESGNGSGNPGGGDTVIVSGTAQYEA
                     PPPVANCNGLNFDDLELKPIRQATVQLLDAGTNAVLDETVTDDNGTFELSAAPDTDAF
                     IRVRAELKRTGGPAWDVDVRDNVTSATNPVPLEQRPLYVLDGSNFDTGSANQVRDLVA
                     STGWGGSSYTATRAAAPFAVLDAVYSGMLLVLAEDPLVNFEPLDVYWSVNNTATAGEP
                     QDIDAGELGTSFYSSDNNKLFLLGMDGVDTEEFDRHVIVHEWGHYFEDTMSRSDSIGG
                     GHSLGDRLDMRLAFGEGFATAYSGMALESSLYCDTLGAAQASGFGIDIESQSAGTPGW
                     FNEISVMKVLYDLWDVADDGADQGSIGFAPIYTVMTGAQAVTPAFTSIFSFAEALKNE
                     NPAVVPFIDGLLQEQNIVASGLNSFGDGETNDGMASDVLPVYTTVVPDGSTINLCSNR
                     QFDTHADGNKLSEHRFLRMNVDLSSAYTFSIVTTTEIPWPDDLDDDRDQSDPDLLILR
                     NGVVQNQVVDGDEQGFSGEANQEIFTTPSAMAAGDYVMALVEFRYQDEESPADFPERT
                     CFDVTVTPAP"
     gene            2120..2773
                     /locus_tag="VFY27_02600"
     CDS             2120..2773
                     /locus_tag="VFY27_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764428.1"
                     /translation="MNVLHRSSMILIAALSACTFAACQDASDVPAERSDSPTETGTPT
                     PAESEGQTSEGAAPGPAAEGASSSSGMSASAGKPSAPVTIDYTVIGTPVVGQPVNINL
                     EVSSSLGSRPVTLSYRINDARNLVFPQAQPQRVALAAPPDTGRAAQQVTVIPQREGRL
                     YLNVSAEVETDEGMMLKSMAIPIQVGSAPQQQQTNGELQEEANGETVISMPADETPR"
     gene            complement(2928..>3125)
                     /locus_tag="VFY27_02605"
     CDS             complement(2928..>3125)
                     /locus_tag="VFY27_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HEX5764429.1"
                     /translation="TEELNGAEEPVPAEEAQVRLQEELVATVEQHGWSVDQYNKVATA
                     ISADPAKRDKTVELINRMISG"
BASE COUNT          628 a          982 c          932 g          583 t
ORIGIN      
        1 ccggtgacca ggatcgtctt gttacccatt ttttgtcgca cccattttgg ctgtcggtcg
       61 caaatcaagt cgcgggattc taccaatcgt cgcagacctt actcgtcgac tatgtcgagg
      121 aaaagaccct aagtcactgt agaaaaaccg gatgtgtcgc aagttattga ttgccaatgc
      181 tgtccctttg gcagcgtcgc ggctatgatc gccgccagcg caccgcaatg taaccattta
      241 gcgttgctca tgtctttaag tcttctgctc tttgcaatcg tccgaggtga cccgtagttg
      301 aaatttttcg cggcaaagcc tgagcgcact tcatgccaac tggcgcttga tcttgcagcc
      361 accgcaatgt gcgcaaccgc cctggtctcc tgcggcggtg gcggcggcgg cggaggaccg
      421 gcggaaagcg gcaacgggag tggtaacccc ggtggcggag acactgtcat cgtaagcggc
      481 acggcacagt atgaagcgcc accgccggtc gccaactgca atggcctgaa cttcgacgac
      541 ctggaactaa agccgatccg gcaggccacg gtacaactcc ttgacgccgg caccaacgcc
      601 gtactggacg agaccgtcac tgacgacaac ggcactttcg agctaagcgc tgcgcccgat
      661 accgatgcct tcatccgtgt ccgcgcggaa ctgaaacgaa ccggcggccc ggcctgggat
      721 gtggatgtgc gggacaacgt gacgagcgcc accaaccccg tgccactcga gcaacgcccc
      781 ctttatgtac tcgacggcag caatttcgat acgggcagcg ccaaccaggt tcgggatctc
      841 gtcgcgagca ccgggtgggg tggttcgagt tacaccgcca cccgcgccgc ggcgccgttc
      901 gccgtactcg atgccgtgta cagcggcatg ctgctggtac tggccgagga tccactcgtc
      961 aacttcgagc ctctcgacgt ttactggagc gtcaacaaca cggcaacggc cggtgagccg
     1021 caggacatcg atgccggcga actcggcact tcgttctaca gcagcgacaa caacaagctg
     1081 ttcctgctcg gcatggacgg cgtggacacc gaggagtttg accgccacgt catcgtgcac
     1141 gagtgggggc actacttcga ggatacgatg tcccgttccg acagcattgg cggtggacat
     1201 tcgctcggcg accggctcga catgcggctg gcgttcggcg agggctttgc gaccgcatac
     1261 tccggaatgg cgctcgagag ctcgctctac tgcgatacgc tcggggccgc ccaggcgagc
     1321 ggtttcggca tcgacattga aagccagtcc gccggcacgc cgggctggtt caacgaaata
     1381 tccgtaatga aggtcctcta cgacctgtgg gatgttgccg acgacggcgc cgaccagggc
     1441 agcatcggct ttgcgccgat ttacactgta atgacgggcg cccaggccgt gacgcccgcg
     1501 ttcaccagca tattcagctt cgccgaggcg ctgaagaatg aaaatcccgc ggtcgtgccg
     1561 tttatcgatg gattgctgca ggaacagaac atcgttgcct cggggctgaa ttccttcggc
     1621 gacggcgaga ccaacgacgg aatggccagc gacgtcctcc cggtttatac gacagtggtg
     1681 ccggacggca gcacgatcaa cctttgcagc aaccgccagt tcgacacgca tgcggacggg
     1741 aacaagctct ctgaacaccg tttcctccgc atgaacgtcg acctgtcctc cgcttacaca
     1801 ttctcgatcg tcaccacgac agagattccc tggccggatg acttggacga cgatcgggac
     1861 cagagcgacc cggacctcct gatactgcga aacggcgtcg tccagaacca ggtcgtcgac
     1921 ggggacgagc agggtttcag cggtgaggcc aaccaggaaa tattcacgac tccgtccgcg
     1981 atggcggccg gcgactacgt catggcgctg gtcgagttcc gttaccagga cgaggagtcg
     2041 ccggcggact ttccggagcg cacctgtttc gatgtgacgg ttacgcccgc gccatgagtg
     2101 acatgacccg aggtgaccaa tgaacgtgct acaccgatca agtatgatcc ttattgctgc
     2161 gctgtctgcc tgtacgtttg ctgcctgcca ggatgcgagc gacgtaccag cggaacgcag
     2221 cgactctcct accgaaaccg ggacgccgac tcccgccgaa tcggaaggcc aaacgagcga
     2281 gggtgccgcc ccaggacccg ccgccgaagg agcgtcatcg tcctccggta tgtcagcgtc
     2341 ggccggcaag ccatcggcgc ctgtcactat cgattacacg gtgatcggga caccggtggt
     2401 cgggcagccg gtcaacatca atctcgaagt gtcgtcgtca ctgggcagcc gtcccgtcac
     2461 gttgagctat cgcatcaacg atgcgcgcaa cctggttttt ccgcaggcgc agccccagcg
     2521 cgtcgcactg gcggcgccgc ccgataccgg gcgcgcagcc caacaggtca ctgtcatccc
     2581 gcagcgggaa ggtcgtctgt acctgaacgt gtccgcggaa gtcgagacgg acgagggcat
     2641 gatgttgaag tcgatggcta tcccgatcca ggtcggcagc gcaccgcagc aacagcaaac
     2701 gaacggtgaa ctgcaggaag aggcgaatgg cgaaaccgtc atctcgatgc ccgccgacga
     2761 aaccccacgc tgaccaggca ccggtaacga acagagcgct gtaggagccc atgcgggcga
     2821 gtgcgccgac gcctgatcgc actcgcccgc atggcgggct acttacagcg tgctaacaag
     2881 aaagggcgcc caaaggcgcc ctggataaag ccgaatttcc tgtccgctta cccgctgatc
     2941 atccgattga tcagttccac cgtcttgtcg cgtttcgccg gatcagcgct gatcgcagtg
     3001 gcaaccttgt tgtactggtc gaccgaccag ccgtgttgct cgacagttgc gacaagttct
     3061 tcctgcaggc gaacctgcgc ttcttctgcc ggtaccggtt cttcggcgcc gttcagttcc
     3121 tcggt
//
LOCUS       DASUOM010000131         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_853790,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000131 DASUOM010000000
VERSION     DASUOM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853790"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="VFY27_02610"
     CDS             complement(<1..1667)
                     /locus_tag="VFY27_02610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5764430.1"
                     /translation="MKTIRLPLVFFIIAAALTACSSETSPGGADLILVNGRVYTLAWS
                     DPGGDGALTKDAPHDGSRWQPDAEAVAIKGDRIVFVGSTSDASSHRGDSTRVVDLAGA
                     TVIPGLVDSHTHVFGLGAALDLVDLVDVDTEEEAVARVAERARSVPKGDWIVGRGWDE
                     GAWANRYPDKVLLSEAVPDHPVFLASLHSFAGWANQMALDEAGITAETEAPVGGEIRR
                     TADGEPNGMFLNRAVPLIGDAVPPPPRETVTKQVLAGLRQMAEDGYVTVHEAGLDARE
                     MSILEELEASGQLPIRVYAMLSLRDEELIREWIARGPDQDSDSMLVTRAVKAFYDGAL
                     GSRGARLLYDYADQPGHRGVSGGDYGFNEALTEEAMQRGFQLAIHAIGDAGNRESLDI
                     LERIFKAHPETARYRHRIEHAQVLHPDDIPRFGKLGVIASMEPPHAMEDKTWAEARLG
                     PERILGAYAWRSLRETGAALTFNSDNPGSDHDIFYGLHSAITRQDKANEPEGGWYPEQ
                     ALNADETLRAYTSWSAFASFREDQTGVLEPGRWADITVMDLDPFVLA"
     gene            1760..>2787
                     /locus_tag="VFY27_02615"
     CDS             1760..>2787
                     /locus_tag="VFY27_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5764431.1"
                     /translation="MYRKDASDAGVRDTACCTVIRLYRCERIVTGKTENPRTDPESIP
                     EHPKVRWTRKYRRALKISTVVLVAHLIGLATSMHALMASRTAPGATAWIVALNATPYL
                     AVPAYWVFGRSKFQDYISLRREVSASLEETLQQELATLEPFRTSWSADDHQAFSAVEK
                     LARLHFVDGNRVELLINGERTFSSIFEGIETAKDYLLVQFYIVRNDELGRQFQHRLIE
                     RAREGVRVHLLLDEIGSGNLSRSYIDELTDAGVEVRWFQSNRGWTNKFQLNFRNHRKL
                     VIADGKRGWIGGLNVGNEYQAEDWRDTHLLVEGPAVLHMQLSFLEDWRWAADDLLQLS
                     WSPHSQSG"
BASE COUNT          532 a          864 c          843 g          548 t
ORIGIN      
        1 tccgcgagca cgaacggatc gaggtccatg accgtgatgt cggcccagcg gccgggctcc
       61 agcacgccgg tctggtcctc gcgaaaactc gcaaaagcgg accagctcgt atatgcccga
      121 agcgtctcgt ccgcgttcaa tgcctgctcc ggataccagc cgccctccgg ctcattcgcc
      181 ttgtcctggc gagtaatcgc cgaatgcagc ccataaaaaa tatcgtggtc cgaccccgga
      241 ttgtcggaat tgaaagtgag cgccgcaccg gtctcgcgca acgagcgcca ggcatacgcc
      301 ccgaggatgc gttccggccc gagtcgcgct tccgcccatg tcttgtcctc catcgcatgt
      361 ggcggctcca tcgaagcaat gactccgagc ttaccgaagc gtgggatgtc atccgggtgc
      421 agcacctgcg cgtgctcgat gcgatgccgg tacctggcgg tctccgggtg tgctttgaag
      481 attcgctcca ggatgtccag cgactcgcga ttgccggcat caccgatggc gtgaatcgcc
      541 agctggaagc cgcgctgcat ggcctcctcc gtcagcgctt cgttgaagcc gtaatcgccg
      601 ccgctgacgc cgcggtgtcc tggctgatcg gcgtaatcat agagaagccg tgccccgcgc
      661 gagccgagtg ctccatcgta aaaggccttt accgctcgcg tgaccagcat gctgtcactg
      721 tcctggtccg ggccccttgc aatccactcg cggataagct cttcatcgcg cagcgacagc
      781 atcgcgtaga cccgtatcgg cagttgtccc gacgcttcca gttcttcgag gatgctcatc
      841 tcgcgtgcat cgaggcccgc ttcatggacg gtcacgtacc cgtcttcggc catttgccgg
      901 aggcccgcga gtacctgctt cgtaacggtc tcgcgcggcg gtggcgggac ggcgtcaccg
      961 atcagcggca cggccctgtt gaggaacatg ccgttcggtt caccgtcggc ggtccgccgg
     1021 atttcaccgc caaccggtgc ctcggtttcg gctgtgatgc ccgcctcatc cagcgccatc
     1081 tggttcgccc agccggcgaa gctgtgaagg ctggccagga acaccgggtg atccggcacc
     1141 gcctcggaca gcaggacctt gtccggatag cgattagccc aggctccctc gtcccagccg
     1201 cgaccgacga tccagtcgcc cttcggcacg ctccgggccc gttcagcgac ccgcgcgacc
     1261 gcttcttctt ccgtgtcgac atccaccagg tcgacaaggt cgagcgcagc gccgagtccg
     1321 aaaacgtgcg tgtgcgaatc gaccagcccg ggaatgaccg tggcgccggc gagatcgaca
     1381 acgcgggtcg aatcaccccg gtgcgacgac gcatcagagg tcgagccgac gaatacgatc
     1441 cgatctccct tgatggctac tgcctcggcg tcaggttgcc agcggctgcc atcgtgcggt
     1501 gcatctttgg tcagcgcgcc gtcgccaccc ggatcgctcc aggccaacgt gtaaacgcgg
     1561 ccattaacaa gaataaggtc ggcgccgccg ggcgaagttt cgctggaaca agcggtcaga
     1621 gcggcggcaa taatgaaaaa gactagcgga agtcgtatgg tcttcattgg ggtgttccca
     1681 ggcgcaagtg cccatccgtt aataatgaca gcttgggcag aagtgaagta ggcattttcc
     1741 acgagtgact ggcttgctct tgtaccgcaa ggatgcctct gatgctggtg tcagggacac
     1801 agcttgttgt actgtcatcc gactttatcg atgcgagcga atcgtgacag ggaagacgga
     1861 aaacccgcgg acggatccgg aatcgatacc tgaacatcca aaagtcaggt ggacgcgcaa
     1921 gtaccgccgc gcgctgaaga tttccaccgt ggtgctggtc gctcatctga tcggtctggc
     1981 cacgtcaatg cacgccctga tggccagccg tacagcgcct ggcgccactg cctggatcgt
     2041 cgctctgaat gcgactccct acctggctgt gcccgcgtac tgggtattcg ggcggagcaa
     2101 atttcaggac tacataagcc tgaggcgcga agtatctgcg tcgctggaag agaccctgca
     2161 acaggaactc gcgaccttgg agccgttcag gacgagttgg tccgcggatg accatcaggc
     2221 tttcagcgcc gtcgagaagc tggcccgcct gcattttgtg gatggcaacc gggtcgaatt
     2281 gctgatcaat ggcgagcgaa cgttttccag catcttcgag ggcatcgaaa ccgcaaaaga
     2341 ctaccttctg gtgcagttct acattgtgcg caacgacgag ttagggcgtc agtttcagca
     2401 ccgcctgatc gagcgcgcca gggaaggggt ccgggtccat ctgcttctgg acgaaatcgg
     2461 cagcggaaat ttgagccggt catatattga tgagctgacc gacgccggtg tcgaggttcg
     2521 ctggttccag tcaaaccgcg gctggacgaa caagtttcag ctgaacttcc gaaaccacag
     2581 gaaactcgtg atcgctgacg gcaagcgcgg ctggatcggg ggactgaatg ttggcaatga
     2641 atatcaagcc gaggattggc gggataccca cttgctggtt gagggacccg ccgtccttca
     2701 catgcagctg tcgttcctgg aagactggcg ttgggcagcg gatgatctgc tgcaactttc
     2761 ctggtccccg cattctcaat ccggcca
//
LOCUS       DASUOM010000132         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_73458,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000132 DASUOM010000000
VERSION     DASUOM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73458"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="glmU"
                     /locus_tag="VFY27_02620"
     CDS             <1..834
                     /gene="glmU"
                     /locus_tag="VFY27_02620"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5764432.1"
                     /translation="KSWLSRLGNANAQGEYYLTDVIGMAAAEGVVVHGVKADDWKEVM
                     GINDRKQLAEAERALQRRLVEELMTQGVSFADPARVDIRGKLECGQDVFIDVNAVFEG
                     DVTLSDNVKIESGNVVRNSRLGAGTVVHPNCHIEGATTGAACEIGPFARLRPGAELGN
                     DVKIGNFVEVKKSTIADGSKVNHLSYIGDTAIGTKVNVGAGTITCNYDGANKHRTVIG
                     DGAFIGSGVELVAPVEVGAGATIGAGSTISKPAPPDTLTVARARQVTVPGWQKPVKKA
                     K"
     gene            936..2768
                     /gene="glmS"
                     /locus_tag="VFY27_02625"
     CDS             936..2768
                     /gene="glmS"
                     /locus_tag="VFY27_02625"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5764433.1"
                     /translation="MCGIVGAVAERNVVPILMEGLRRLEYRGYDSAGLAVMEGERLTR
                     VRRLGKVQELQNALDASPLHGATGISHTRWATHGAPSEINAHPHMSRDDIAVVHNGII
                     ENYQELREDLLRRGFRFESETDTEVVAHRIRYHLEELKDLPAAVRATVAELEGAFALV
                     VMAASDPHKLVLARAGCPVVIGLGIGENFVASDVAALLPVTRKFMFLDEGDTAVVERE
                     KVTIRDAGGTEVSRPVKESELTADVAERGNYRHYMQKEIHEQARAVARTLEDRIANGK
                     VLDPAFGRDAAQALDRVRGVHIVACGTSYHAGLVARYWIEQLCRRPCSVEIASEYRYR
                     SPVVPEGTLFVTVSQSGETADTLAALRAAKELGYVSTLAICNVPESSMVRESELVMMT
                     RAGPEIGVASTKAFTTQLAALALLTIALARRNGLEEKQERALVAQLSELPGLIEQVLT
                     LDGAIESLAQHFVDKKHALFLGRGVQNPVAMEGALKLKEISYIHAEAYAAGELKHGPL
                     ALVDEDMPVVAIAPDDQLLEKLKSNLQEVRARGGQLFVFADPRVQFGDRVGIHTMRMP
                     AMIQDFPSPILYTIPLQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
BASE COUNT          563 a          834 c          937 g          509 t
ORIGIN      
        1 aagtcctggc tgtcgcgcct cggcaacgcg aatgcgcagg gagaatatta cctgacggat
       61 gtcatcggga tggccgccgc ggaaggcgtc gtggttcacg gcgtgaaggc ggacgactgg
      121 aaggaagtta tgggtatcaa cgacaggaaa cagctggccg aagcagagcg cgcattacaa
      181 aggcgcctgg ttgaggaact gatgacgcaa ggcgtcagtt ttgccgatcc tgcccgcgtc
      241 gacattcgcg gcaagctcga gtgtggccag gacgtcttta tcgacgtcaa cgcggtgttc
      301 gaaggcgacg tcaccttgag cgacaacgtg aagatcgagt ccgggaacgt cgtgcggaac
      361 agccgcctcg gtgccggaac ggtcgtgcat ccgaactgcc acatcgaggg tgcgaccacg
      421 ggggcagcct gcgagatcgg gcccttcgcg aggctccggc cgggcgccga acttgggaac
      481 gacgtcaaga tcggtaactt cgtggaggta aagaagagca cgatagcgga tggcagcaag
      541 gtgaaccacc tgtcatacat tggcgatacc gccataggta cgaaggtcaa cgtgggagcc
      601 ggcaccatca cctgcaatta cgacggagcg aacaagcatc ggaccgtgat cggcgacggc
      661 gcctttatag gctccggagt ggagctcgtc gcgccggtgg aagtcggggc gggtgcgacc
      721 ataggcgcgg gctcgaccat atcgaagccg gcacccccgg atacccttac cgtcgcgcgg
      781 gcgcgccagg tcacggtccc cggctggcag aagcctgtca aaaaggcgaa gtaacagctc
      841 ctgttgcggc cgattgctca tttccgtgaa gtgttgcaat ctgggcggcc aatttcgctg
      901 cgatactggc gtgcgttgcc agaggctcta ttgatatgtg tggaatcgtc ggcgccgttg
      961 ccgaaagaaa cgtcgttccg atcctgatgg aaggcctgag gcgcctcgag tatcgcggct
     1021 atgactctgc cgggctcgcg gtcatggagg gcgagcggct gacccgcgtg cgtcgcctgg
     1081 gaaaggtcca ggagctgcaa aacgcgctcg atgcgtcccc gttgcacggc gccaccggca
     1141 tatcgcacac gcgctgggcg acacacggag cgccgagcga aatcaatgcg catccccaca
     1201 tgtcccgcga cgacatcgcg gtggtgcaca acggcatcat cgaaaactac caggaactgc
     1261 gcgaggatct cctgcgccgc ggcttcaggt tcgagtcgga gaccgacacc gaagtggttg
     1321 cgcatcgcat ccgctatcac ctggaagagc tcaaagacct gcctgccgcc gtcagggcga
     1381 cggtggctga gctcgaaggc gcttttgcgc tggtcgtaat ggccgctagc gatcctcaca
     1441 agctggtgtt ggcccgagct ggctgcccgg tcgtcattgg cctcggcatc ggcgaaaact
     1501 tcgttgcatc ggacgtggcc gccttgttgc cggttacccg gaagttcatg ttcctggacg
     1561 aaggcgatac cgcggttgtc gagcgcgaaa aggtgacgat ccgggatgcc ggtggaactg
     1621 aagtttcgcg cccggtaaaa gaaagcgaat tgacggcgga cgtcgccgag cgtggcaact
     1681 accgtcacta catgcagaag gagattcacg agcaggcacg tgccgtcgcg cgcacgctcg
     1741 aggaccgtat tgcaaacggt aaggttctcg atccggcgtt cggtcgcgac gctgcgcagg
     1801 cgctcgatcg cgttcgcggc gtgcacatcg tcgcctgcgg gaccagttat cacgccgggt
     1861 tggtggcgag gtactggatc gaacaactgt gccgcaggcc gtgcagcgtc gagatcgcga
     1921 gcgagtaccg ctatcgctca ccggtggttc cggaaggaac gctgttcgtc acagtcagcc
     1981 agtccggtga aacggcggat acgctcgctg ccttgcgtgc ggcgaaggaa ctgggctacg
     2041 tctccacgct ggcgatctgc aacgtgccgg aaagctcgat ggtccgcgaa tcagagctgg
     2101 tcatgatgac gcgcgccggt cccgagatcg gcgttgcgtc gaccaaggcc tttactaccc
     2161 agctcgccgc gctggcgctg ctcaccatcg cgcttgcgcg gaggaacggc ttggaggaga
     2221 agcaggagcg tgcgctcgtc gcgcagcttt cggaactgcc tggtcttatc gaacaggtcc
     2281 tgacactgga cggcgcaata gaatcgctcg cgcaacattt cgtcgacaag aagcacgcgt
     2341 tgtttctcgg acgcggcgtg cagaatcccg tggccatgga gggcgcgctc aagctcaagg
     2401 aaatctccta cattcacgcc gaggcctatg cggccggcga actgaagcac ggtccgctcg
     2461 ctcttgtgga cgaggacatg cccgtggtcg ccatcgcacc ggacgaccag cttctggaaa
     2521 aactcaagag caacctgcag gaagtgcgcg cgcgtggcgg ccagctgttc gtgttcgccg
     2581 atccgcgggt gcagttcggc gaccgcgtgg gcatccacac gatgcgcatg ccggcgatga
     2641 tccaggactt cccgtcgccg atcctgtaca cgatcccgct gcagttactc gcgtatcacg
     2701 tcgcagtgct gaaaggcacc gatgtcgatc agccgcgtaa cctggcgaag tcggttacgg
     2761 ttgaatagtc cggtaaccga gcgcggcgag atcgtactct cctcctgttg tccttgagat
     2821 gggcgagcgc tctgtgaata atc
//
LOCUS       DASUOM010000133         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1318625,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000133 DASUOM010000000
VERSION     DASUOM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318625"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..665
                     /locus_tag="VFY27_02630"
     CDS             30..665
                     /locus_tag="VFY27_02630"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX5764434.1"
                     /translation="MAKLYFYYSSMNAGKSTSLLQSSYNYKERGMNTLILAPHFDDRY
                     GVGRVTSRIGLESRATTFGKADNLFDIVSACCAEDPLHCVLIDEAQFLTKKQVFQLGE
                     VTDELEIPVLAYGIRTDFQGEPFEGSQYLLAWADNLTELKAICHCGRKATMVLRLDEN
                     GNAVTEGSQVEIGGNDRYISMCRKHFRENFYREMLGETQKARIARVVGAVS"
     gene            complement(672..1949)
                     /locus_tag="VFY27_02635"
     CDS             complement(672..1949)
                     /locus_tag="VFY27_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5764435.1"
                     /translation="MELSEAGLRQQPFRAHGKPLAAVTYAAHVAALDFLTATYADDHG
                     LGLFQGPPLSGKSVIIKQFTESLGDETAYAVVDGAGAETDALLRDILRQFGYDLQFDT
                     VNELTNMLKVFVVQQTVSNHAPLLVVENTHAMNPLALSTLCELVALKVHEKSALRVIL
                     ASDRSMAAMVGAPAMSCIAARVTGSFFVEPLSDFETKNYVYEKMLAAGCPAPQTVFPG
                     EVCLALHSAAGGWPGIVDQLALQALRKAEKFPVTTDLIEQPPKPEDIRTASDAAASRP
                     SHAPKLFVTQNGKTISAIDFQGERLLVGRSEHNELRINSRFISRHHALFVRHENSTFL
                     MDLNSTNGTFVNSRRISNLMLKNDDIVLIGNHRIKFVDPTATERTPLDDAGFSETIAM
                     KNLEDIRQLLAREQICRDTDDVAVPRPRAADGA"
     gene            2223..2834
                     /gene="ccmA"
                     /locus_tag="VFY27_02640"
     CDS             2223..2834
                     /gene="ccmA"
                     /locus_tag="VFY27_02640"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX5764436.1"
                     /translation="MAELSGYDLALIRGNRYLFQGLNFSAGSGELLVVEGPNGSGKTS
                     LLRAIAGLIDPDEGVVWWNNTNVKRNRQSFHALLVWMGHRPGFKGDLTLVENLRFEAG
                     LRSMRMSTAEHQLRRLKIATLADIPFRALSAGQQRRVALARMCLADAPLWLMDEPFTN
                     MDAEGQALVVDVLREHLRDGGMCVMATHQRFEIDATVRRIELT"
     gene            2864..3529
                     /gene="ccmB"
                     /locus_tag="VFY27_02645"
     CDS             2864..3529
                     /gene="ccmB"
                     /locus_tag="VFY27_02645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5764437.1"
                     /translation="MAGLAAVVRRDLHLAWRRPGDVLNPLFFFAIVSTLFPLAIGPDA
                     EQLALNGPGILWVAALLAALLSLNTVFLNDHEDGTLDQLLLSPQPLPLLAFAKSMAHW
                     LMSGLPLVLASPLVALSLQLPARATTVMMLTLALGTVSLSMLGAIGAGLTVGLNRGNA
                     LLSLLILPLAMPMLIFGARTVSLAAAGDAASAGVYFLGAYCVLALSLAPFATAAALRI
                     SNE"
     gene            3550..4299
                     /locus_tag="VFY27_02650"
     CDS             3550..4299
                     /locus_tag="VFY27_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX5764438.1"
                     /translation="MWTFLHKLGSPPYFYGLAASMIPWLTAAGLVLVGYGTVAGLFLA
                     PPDYQQKDAFRIIYVHVPAAYLSMLGYVVMAAAAGIGLIWRMKMAHAVAASAAPLGAW
                     FTFLALITGAVWGRPMWGTWWEWGDPRLTSELILLFLYFGYMALRAAIDDTSKADRAS
                     AVLALVGAVNVPIIHFSVEWWSSLHQGPTLVRAGGPAIAGPMLWPLLAMIFGFSLLFA
                     ALLLGRVRTEVLIRERRARWVRKLVYPEETA"
     gene            4299..4472
                     /gene="ccmD"
                     /locus_tag="VFY27_02655"
     CDS             4299..4472
                     /gene="ccmD"
                     /locus_tag="VFY27_02655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX5764439.1"
                     /translation="MEVLSMGGYGVYVWTAYALTFGVLILCVVQGRRRHRRVLDELRT
                     RLLMDRSAAEKSK"
     gene            4469..4930
                     /gene="ccmE"
                     /locus_tag="VFY27_02660"
     CDS             4469..4930
                     /gene="ccmE"
                     /locus_tag="VFY27_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5764440.1"
                     /translation="MKPRQQRMLAVGLAAAGLLIATALTLRAFQDNMMFYLEISEVTA
                     GKVPEDRNFRVGGLVVEDSLTRQDGELDVQFVLTDTVNQLPVVYSGVLPDLFREGQGI
                     IAHGRMNDDGQFVADTVLAKHDETYMPPEVADSLAKHAEAKAAAARAGSDN"
     gene            4927..>5307
                     /locus_tag="VFY27_02665"
     CDS             4927..>5307
                     /locus_tag="VFY27_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HEX5764441.1"
                     /translation="MIPEIGHFALILALSLALCQGAMPLLGARRGDAAMMAVARPAAL
                     GQFVFVVIAFMCLAYAFLTSDFSVLYVANNSQLALPTAYKFSAIWGAHEGSLLLWILI
                     LSAWTIAVGRFSEELSDSFAARVIG"
BASE COUNT          963 a         1531 c         1674 g         1139 t
ORIGIN      
        1 tccacccggt gaattcctga gaaacggtca tggccaaact ctacttctat tactcgtcga
       61 tgaacgcggg caagtcgact tccctgctgc aatccagtta caactacaag gagcggggca
      121 tgaatacgct gatcctcgcg ccgcacttcg acgaccgcta cggcgtgggc agggtgacct
      181 cgcgcatcgg cctggaatcg agggcaacga cgttcggcaa ggcagacaac ctgttcgaca
      241 tcgtttccgc ctgctgcgcg gaagacccct tgcactgcgt cctgatcgac gaagcgcaat
      301 tcctgacgaa gaagcaggtt ttccagctcg gcgaagtaac cgacgagctc gagatcccgg
      361 tgctcgcata tggcatacgc acggatttcc aaggagagcc tttcgagggc agccagtatc
      421 tgcttgcctg ggcggataac ctgaccgaac tgaaggccat ctgccattgc ggccgcaagg
      481 cgaccatggt gttacggctg gacgagaacg gcaatgccgt taccgaaggc tcgcaggtgg
      541 aaatcggcgg caacgacagg tacatctcga tgtgccgcaa gcacttcagg gaaaacttct
      601 accgcgagat gctcggcgaa acgcagaagg cgcggatcgc gcgggtggta ggcgccgtca
      661 gctgatcgtc tttacgcgcc gtcggcggcg cgggggcggg ggaccgcgac gtcatccgtg
      721 tccctgcaga tttgttcgcg ggccagcagt tggcgaatgt cttccaggtt cttcatggcg
      781 atggtttcgc tgaagccggc atcgtccagc ggcgtccgct ccgttgcggt cggatcaacg
      841 aacttgatgc ggtgattgcc gatcagcacg atgtcgtcgt tcttcagcat caggttggag
      901 atgcggcgcg aattgacgaa cgtgccattc gtactgttca ggtccatcag gaaggtcgag
      961 ttctcgtgcc tcacgaacag ggcatggtgg cggctgataa accggctgtt gatacgcagt
     1021 tcattatgct ccgagcgccc gaccagcagg cgctcgccct ggaaatctat cgccgaaatc
     1081 gtcttgccgt tttgcgtcac aaagagctta ggcgcgtgcg atggccgtga cgcggcagcg
     1141 tcggaggcag tccttatgtc ttcgggtttg ggcggctgct cgatgagatc cgtggtaaca
     1201 ggaaattttt ccgcctttct cagcgcctgt agtgcgagct ggtccacgat gcccggccat
     1261 cctccggccg cggagtgcag ggcgaggcac acctcccccg gaaacaccgt ctgcggagcc
     1321 gggcagccgg cggcgagcat cttctcgtac acgtaattct tggtttcgaa gtccgagagt
     1381 ggttcgacaa aaaagctgcc ggttacacgc gcggcgatac acgacatcgc gggggccccg
     1441 accatcgccg ccatggagcg gtcgcttgcc agaatcactc gaagggcgct tttttcgtgt
     1501 accttgagcg ccaccagttc gcagagcgtg ctgagcgcga gggggttcat cgcgtgcgta
     1561 ttctctacaa ccagcaatgg agcgtgattg gacacggtct gttgcacgac gaaaaccttc
     1621 agcatgttgg tcaattcgtt gaccgtgtcg aactggaggt cgtagccgaa ctggcgaagt
     1681 atgtcccgga ggagcgcatc cgtttcggca cccgcgccgt cgacgacagc gtacgccgtc
     1741 tcgtcgccga gcgactccgt gaactgtttt atgatgacag acttgccgga cagcggaggt
     1801 ccctggaaca ggcccagtcc atgatcgtcg gcatacgttg ccgtgaggaa atccagtgcc
     1861 gcaacgtgcg cggcgtaggt gactgccgcg agaggcttgc cgtgagcacg aaaaggttgt
     1921 tgccgaagtc ccgcttcgct cagttccatt ttcttgaacc tggttcgtct ttatcgggaa
     1981 agttttgtca tgctgataca gttactttcc cgttcttctt aaagttcaaa ggcagctgag
     2041 acacgagtct aaggtgaagc gccgcgtttt gtaaacttgt ttgcgcgcgt tttaaacttt
     2101 caatcggtaa tactgcttaa ccgatataaa aaattaacta aagtgtttaa gtgactgata
     2161 attctaataa accaacgcgc ctccgattaa ggtgtgtgcc cgcatcatcg gagagcgcgt
     2221 gaatggctga actgtcaggt tacgacctgg cgctcatccg gggtaaccgc tacctgttcc
     2281 agggattgaa cttctcagcc ggtagcggcg aactcctggt agtcgaggga cccaacggca
     2341 gcggcaagac gagcctgttg cgcgccattg ccgggcttat cgatcccgac gagggcgtgg
     2401 tgtggtggaa taatacaaac gtcaagagga atcgccagtc ctttcatgca ctgctggtgt
     2461 ggatggggca ccggcccggc ttcaaaggcg acctgacgct cgtcgagaac ctgcgtttcg
     2521 aggcgggact gcgcagcatg aggatgtcga cggcggagca tcagctccgg cgcctgaaga
     2581 tagcgacgct tgccgacatt ccctttcgcg ccctgtcggc gggccagcag cgccgggtcg
     2641 ccctcgcgcg gatgtgcctt gccgatgcgc cgttgtggct gatggacgaa cccttcacca
     2701 acatggatgc agaagggcag gcgctggtgg tggacgtcct gcgggagcat ttgcgggatg
     2761 gcgggatgtg tgtaatggcg acgcaccagc gtttcgagat tgacgcaaca gttcgacgga
     2821 tcgagcttac gtgacggagc ctgcgccaaa gctgccagga ctgctggccg gccttgcagc
     2881 ggtagtgcgg cgtgacctgc atcttgcctg gcgccggcct ggcgatgttc tgaacccgtt
     2941 gttcttcttt gccattgtca gcacgctgtt tccgctcgcc atcggccccg atgccgaaca
     3001 gcttgcactg aacggcccgg ggatactgtg ggttgccgct ttactggcgg ccttactctc
     3061 attgaacacc gttttcctga acgaccacga ggacggcacg ctcgaccagt tgctgttgag
     3121 tccgcagccg ctgccgctcc tggcgttcgc gaaatccatg gcgcactggc taatgagcgg
     3181 ccttccgctg gtgctggcat cgccgctcgt ggcgctgtct ttgcaactgc ccgcgcgggc
     3241 gacaacggtt atgatgctca ccctcgcgct ggggacggtc agcctgagca tgcttggagc
     3301 catcggggcc gggctgactg tcggcctgaa ccgcggcaac gcgctgttga gtttgttgat
     3361 attgccgttg gcgatgccga tgctgatctt cggcgcgcgc acggtttcac tggccgctgc
     3421 aggagatgcg gcatcagccg gtgtctactt cctcggcgcg tattgcgtac tggccttgtc
     3481 gctggccccg ttcgccaccg ccgccgccct gcggatcagc aatgaatagg ttcgacgaag
     3541 actgacagta tgtggacgtt cctgcacaag ctcggctccc cgccgtattt ctatggcctt
     3601 gcggcatcga tgatcccgtg gctcacggct gcggggcttg tactggtagg ttacggcacg
     3661 gttgccggcc tttttctcgc accacccgac taccagcaga aagatgcgtt tcgcatcatc
     3721 tacgtgcatg tgccggcggc ttacctctcg atgctcggct atgtcgtgat ggctgcagcg
     3781 gccggcatcg ggctgatctg gcggatgaaa atggcacatg cggtggcggc cagcgccgct
     3841 ccgctcggcg catggtttac cttccttgcg ctgatcaccg gcgccgtctg gggcaggccg
     3901 atgtggggca catggtggga gtggggcgat ccgcgcctga cgtcggagct gatactgctg
     3961 ttcctttatt tcggctacat ggcgcttcgg gcggccatcg acgataccag caaggcggac
     4021 cgcgccagcg ctgtgctggc actggtcggc gccgtcaacg tcccgatcat ccacttttcc
     4081 gtagaatggt ggagttcgct gcaccagggg ccgacgctcg ttcgtgccgg cggaccagcc
     4141 atcgcgggac ccatgctgtg gccgctgctg gccatgattt tcggattttc gctgctgttc
     4201 gcggcgcttc tgctgggccg ggtgcgcacc gaagtgctga tccgtgaacg gcgtgcaagg
     4261 tgggtccgca aactggtata tcctgaggaa accgcctgat ggaagtcttg tctatgggtg
     4321 gttacggtgt ctatgtgtgg acggcctacg cgctcacttt cggagtactc attctctgcg
     4381 tggtacaggg caggcgccgc caccgcaggg ttttggatga actgcggacg cggctgctga
     4441 tggaccgttc ggctgcggag aaaagtaaat gaaaccaaga cagcaacgca tgctcgcggt
     4501 cggcctcgct gccgcggggc tgctcattgc aacagctcta acgcttcgag ctttccagga
     4561 caatatgatg ttttacctgg agatatcaga agtaaccgcg ggcaaagtac cggaggatcg
     4621 aaacttccgc gtcggcggcc tggtcgtcga ggacagcctc acgcgccagg acggcgaact
     4681 cgacgtgcag ttcgtgctga cggatacggt gaaccaactg ccggtagttt acagcggcgt
     4741 gctgccggac ctgtttcgcg aagggcaggg gatcatcgcg cacgggcgca tgaacgacga
     4801 cgggcaattc gtcgccgaca cggttctcgc gaagcatgac gaaacctata tgcccccgga
     4861 agtcgcagat tcgctggcga aacacgccga ggcaaaagcc gccgcggccc gggccggtag
     4921 cgacaattga ttccggagat cggacatttc gcgctgatcc tcgcgctgtc cctcgctctt
     4981 tgccagggcg cgatgccgtt gctcggggcg cgccgtgggg atgcagcgat gatggccgtc
     5041 gcaagaccgg cggcgctggg gcagttcgtg ttcgtggtca tcgcgttcat gtgcctggcc
     5101 tacgcgtttc tcaccagcga tttttcggtt ctctacgttg ccaacaattc acagcttgcc
     5161 ctgccgaccg cttacaagtt ctcggcgatc tggggcgcgc acgaagggtc gctacttctc
     5221 tggatactga tcctgtcagc gtggacgatc gcggtcgggc gcttcagcga ggaactgtcg
     5281 gattctttcg cggcgcgtgt cattggt
//
LOCUS       DASUOM010000134         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1523923,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000134 DASUOM010000000
VERSION     DASUOM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523923"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VFY27_02670"
     CDS             <1..1505
                     /locus_tag="VFY27_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX5764442.1"
                     /translation="LTEKLLLYGGAIQLAGTVKGRKASRHATSDWMALEQQRGISVTS
                     SVMQFPYRDRLVNLLDTPGHEDFSEDTYRTLTAVDSALMVIDCAKGVEQRTIKLMDVC
                     RLRDTPIMTFVNKLDRDGRPPVDLLDEIESVLRIQCSPVTWPIGMGRSLKGVYHLLAD
                     RIYLYSRAGREKASSIEMIEGLDSVRAADVLGDDAERFREEIELVKGACPALDMDAYL
                     AAQQTPVFFGSAINNFGVKELLDAFVEHAPSPRPRETETRLIGPAEEKLTGFVFKIQA
                     NMDPGHRDRIAFMRICSGKFEKGMKLTHVRSGKEMKIPAAITFMAADRLQTDTAYAGD
                     IIGLHNHGTINIGDSFSEGEEIRFRGIPSFAPEIFRRAILKDPLKLKALQKGLSQLCE
                     EGATQLFKPLRNNDLILGAVGQLQFDVVAHRLRDEYKVECQFEPISVATARWIEAGNE
                     QKLSEFRKKAHDNLALDHSDRLVYIAPTRINLNLAEERWPDISFRETREH"
     gene            1524..3473
                     /locus_tag="VFY27_02675"
     CDS             1524..3473
                     /locus_tag="VFY27_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5764443.1"
                     /translation="MADEIKPLKLDKDPTSAKSIPLAEYFRLLDALPAAAYACDAEGL
                     ITYFNQRAADLWGREPKLNEPTERFCGSIRLFSPDGDPIEHNECWMALAVQEDRAYDD
                     LDIVIQRPDGSRVFGVAHAHPLHDESGVLIGAVNVVVDVSERKRGEEAQGRLVAIVES
                     ADDAIIGKDLNGVITDWNSGAERLFGYSAAEAVGRPVSMLIPSDRMNEEPDILRRIRG
                     GETISHYETIRRRKDGTLLNISLSVSPIHDAHGRIIGASKIARDIAGQKKMEEALREA
                     DRRKDEFLAMLSHELRNPLATIRNSVQLLRMDPGHAMQRKVLAPLERQVSTLTRLVDD
                     LMDVSRVTMGRVELQRADVGIGRVLRDAAESVRRDMRDRGHSFKISIPATDVVVHADA
                     VRLEQVVVNLLTNAAKYTSDGGTIELSAERDGEDAVLRVRDSGIGIQPELLEEVFELF
                     SQGETTLDRAKGGLGIGLALVKYLVEMHGGSVQAKSDGPGTGSEFIVRLPAVAADEQA
                     KRILQDALAAEGGVQEHALRVLVVDDNRDSADLQAALLQHNGHKVMTAYNGRDALEVA
                     VSFRPEVILLDLGLPEVDGYEVAYRLRQQPELDGVVLVAMTGYGQPEDRQRSQAVGFD
                     HHLVKPAEFADLQNILTSVQDMKLP"
     gene            complement(3532..>3866)
                     /locus_tag="VFY27_02680"
     CDS             complement(3532..>3866)
                     /locus_tag="VFY27_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5764444.1"
                     /translation="DWRKMVLTLLAILLLLIGGLSLLLFRRYRPPRKDEAALLYRQFI
                     RKLPIGPRSGETPLQYASRIASAGAANRGEVDTVTERYLVARYGAPDTGALPELRRAV
                     RTFRKRTL"
BASE COUNT          791 a         1111 c         1232 g          732 t
ORIGIN      
        1 cgctgaccga aaagctcttg ctctacggcg gtgcgatcca gctggcgggc accgtgaaag
       61 gccgtaaggc gagccgtcat gccacatccg actggatggc gctagaacag cagcgaggaa
      121 tctcggtcac gtcttccgtg atgcagttcc cgtatcgtga ccggctggtc aacctgctgg
      181 acacgccggg gcacgaggat ttctcggaag atacctaccg cacgctcact gcagtcgatt
      241 ccgcgcttat ggtcatcgac tgcgcgaaag gcgtcgagca acgcaccatc aagctgatgg
      301 acgtttgccg gctgcgcgac accccgatca tgacgttcgt caacaagctc gatcgggatg
      361 gtcgcccgcc tgtcgatttg ctggacgaga tcgagtccgt gcttcggatc cagtgttcgc
      421 cggtaacgtg gccgatcggc atgggacgct cccttaaagg cgtctatcac ctgctcgccg
      481 atcgtatcta tctctattcg cgcgcgggtc gtgagaaggc ttcgtccatc gagatgatcg
      541 agggcctgga ctcggtgcgc gcggccgacg tgcttggcga tgacgcggag cgcttccgcg
      601 aagagatcga attggtcaag ggagcttgtc cggcactgga catggatgcc tatctggcgg
      661 cacagcagac gccggtgttc ttcgggtctg ccatcaacaa tttcggcgtg aaagagttgc
      721 ttgatgcatt cgtcgagcat gcgccctcgc cgcgaccgcg tgaaacggaa acgcgactga
      781 tcgggccagc cgaagaaaag ctgaccggct tcgtcttcaa gatccaggca aacatggacc
      841 cgggccaccg tgaccgcatt gctttcatgc ggatttgctc cggaaagttc gagaagggca
      901 tgaaactcac gcacgtgcgc tcgggaaagg agatgaagat cccggctgca atcacgttca
      961 tggccgcaga ccggctgcag accgacacgg catacgccgg tgacataatc ggtttgcaca
     1021 atcacggcac gattaacatc ggtgacagtt tttccgaagg cgaagagatc cgctttcggg
     1081 gcatccccag tttcgctcca gagatatttc gacgggcaat actcaaagat ccgttaaagc
     1141 tcaaggccct acagaagggt ctttcgcaac tgtgcgaaga aggcgcgacc cagctcttca
     1201 agccgctacg caataacgac ctgattctcg gagccgtggg tcaattacag ttcgacgtag
     1261 ttgcgcaccg tttgagggat gagtataaag ttgagtgcca gttcgagccg atcagcgtcg
     1321 caacggcgcg ctggatcgag gccgggaatg aacaaaagct gtccgagttc cggaaaaagg
     1381 cgcacgataa cctggcactg gaccacagcg accgtctcgt ctacatcgcg ccgacgcgca
     1441 tcaacctcaa ccttgcagag gagcgctggc cggatatcag tttccgcgag actcgcgagc
     1501 actaggggat gtcggaatag caggtggcgg acgagataaa gccattgaag ctcgacaagg
     1561 atccgacgag tgccaaaagc attcctctgg cggaatactt tcgtctcctc gacgcactgc
     1621 cggccgccgc atacgcctgc gatgcagaag gcctgatcac ttacttcaac cagcgcgccg
     1681 cagacctgtg ggggcgcgaa ccgaagctca atgagccgac cgagcgcttt tgcggttcta
     1741 tccggctttt ctcgcctgat ggcgatccga ttgaacataa cgagtgctgg atggcgctcg
     1801 ccgtccagga agatcgcgct tacgacgacc tcgacattgt catccagcgc cccgacggca
     1861 gccgtgtttt cggggtggcg cacgcccacc ccctgcatga tgagagcggc gttcttatcg
     1921 gtgcggttaa cgtggttgtc gatgtcagcg agcgcaagcg gggggaggaa gcgcagggga
     1981 ggctggtcgc catcgtcgag tcggcagacg acgcgatcat cggcaaggac ctgaacggcg
     2041 tcatcaccga ttggaattcc ggggcggaaa ggcttttcgg ctacagcgcc gcggaagcag
     2101 ttggccggcc ggtcagcatg cttattccgt ccgaccgcat gaatgaggaa ccggatatcc
     2161 tgcggcgcat ccgaggcggc gagacgatca gccattacga aacgatacgt cgccgcaagg
     2221 acggaacgct tctgaacatt tcgttgagcg tatcgcccat ccatgatgcg catggcagga
     2281 tcatcggtgc ttccaagata gcgcgcgata tcgctggcca gaagaagatg gaagaggcgt
     2341 tgcgggaggc agaccgccga aaggacgagt tcctcgccat gctttctcat gagctgcgta
     2401 atccgcttgc gaccattcgc aattcggtac aactgctccg gatggatcca ggccatgcaa
     2461 tgcagcgcaa ggtgctggcg ccgctggagc ggcaggtatc gacgttgacg cgcctggtcg
     2521 atgatctcat ggatgtttcc agggtcacca tggggcgggt tgagctgcag cgtgcggacg
     2581 tcgggatcgg cagggtcttg cgggacgctg cggagtccgt gcgtcgagat atgcgggacc
     2641 gcggtcactc gttcaaaatt tcgattcccg caaccgacgt tgtcgtgcat gcggatgccg
     2701 tcaggctgga acaggttgtc gttaatctgc tcacgaacgc ggccaagtac acttccgacg
     2761 gcggaaccat agaactgtcg gcggagcgtg atggcgagga cgcagttttg cgggttcggg
     2821 attcgggcat tggcatccag ccggaacttc tcgaggaggt tttcgagttg ttttcccagg
     2881 gcgaaacgac cctggatcgc gccaagggcg gcctgggaat agggctcgcg ctcgtgaagt
     2941 atctcgtgga aatgcatggg ggaagcgtgc aggcaaagag cgatggtccg ggcaccggga
     3001 gtgagttcat cgtgcgcttg cctgctgttg cggccgatga gcaggcgaaa agaattttgc
     3061 aggatgcgct ggctgccgag ggcggcgtgc aggagcatgc gttgcgcgtt ctcgtggtgg
     3121 acgacaaccg cgattcagcc gacctccagg cggcgctgct acagcacaac ggacacaagg
     3181 tgatgacagc ctacaacgga agggacgcgc tcgaggttgc ggtgagcttc cgtccggagg
     3241 tgatcctgct cgacctcggc cttcccgagg tcgacggcta cgaggtcgcg taccgcctac
     3301 ggcagcagcc ggagctcgat ggcgtggtgc tggtcgccat gacagggtat ggccagccgg
     3361 aagaccggca gcgctcgcaa gccgtgggct tcgatcatca cctcgtaaag ccggcggagt
     3421 tcgccgacct gcagaacatc ctgacctccg tgcaggacat gaagttgccg taggagccgc
     3481 catgcgggcg agtgcgacac actccggcga actcgcctgc atggcaggct cctacagcgt
     3541 tcttttcctg aaagtcctga ccgctctgcg caattccgga agcgcgcctg tatccggtgc
     3601 gccgtaacgc gccaccaggt accgttcagt aaccgtgtcg acttcgcccc ggttcgccgc
     3661 acctgccgag gcgatccgtg acgcgtactg gagtggtgtt tcaccggacc gcggaccgat
     3721 cgggagtttg cggatgaact gccggtacag aagcgccgct tcgtccttcc ttggcggccg
     3781 gtagcgccgg aagagcagga ggctcaagcc accgatcagc aggagcagga tcgccagcaa
     3841 cgtcagcacc atcttgcgcc agtccg
//
LOCUS       DASUOM010000135         7899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1476128,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000135 DASUOM010000000
VERSION     DASUOM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476128"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="ggt"
                     /locus_tag="VFY27_02685"
     CDS             complement(<1..1337)
                     /gene="ggt"
                     /locus_tag="VFY27_02685"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5764445.1"
                     /translation="MLRLCKLLSLEATLLLVLACSPPTEAPEAVATAAEAVNREGGDE
                     AAVAMPDRYAAEVSEAILKGGGNAVDAAIAAGFTLAVTYPEAGNIGGGGFMLIHMKGE
                     STFLDYRETAPLAADRDMYLDENGDVIPGASLIGHKAVGVPGTVAGFWAAHQRYGTLP
                     WEDLVKPAARIAEEGFVVPESLGGGMKTMIDWFSDKTNFGEYFGDLESGKVFRQPELA
                     ATLWRIAEQGPADFYEGETAKLIVEEMERGDGLITLEDLERYKAVWRAPLRAEWRGYE
                     ILSAPLPSSGGFAVIQLLSMKDYLNHEFEGLPHNSPQYIHLVAEIEKRVFADRAEYLG
                     DPDYVDTRIDRLVSEEYMMQRAAEVDTDEISAMVSAGTVANESPDTTHYSIVDQWGNA
                     VAQTYTLNISFGSGVVVEGAGFLLNDEMDDFSVKPGVPNTYGVTGNEANEIQPG"
     gene            complement(1411..2520)
                     /locus_tag="VFY27_02690"
     CDS             complement(1411..2520)
                     /locus_tag="VFY27_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764446.1"
                     /translation="MTPRSCDHAHEYPAYIKRTRMVATRAREEVLYEFDDRPDAGETM
                     RVAPGVVWLRMPLPFALGHINLWLLEDDGAWAIVDTGVFTDITRGVWENTFSEAMQDR
                     PLSRVFVTHLHPDHSGCAGWLTEQRDVELWMPREEYLLCRILVADTGRPTPEAGIRFY
                     NAAGFPPEAMSYYQKMFGMFGKYVAALPESYRRLKDGDTVRIGANEWQVVIGRGHSPE
                     HACLFCEGLNVLISGDQLLPTISSNVSVYPTEPAANPLDDWITSLAEMKSRIPADVLV
                     LPSHGKPFRGAHERLQELIDEHHDCLDELAVLCREPKRAVDAFPALFRSPINENNLIM
                     ATGEAVAHLNYLLYQGRMAVQTDADGVNWYRTSMR"
     gene            complement(2660..6151)
                     /locus_tag="VFY27_02695"
     CDS             complement(2660..6151)
                     /locus_tag="VFY27_02695"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5764447.1"
                     /translation="MVTATSLAEATRIDSYELDDRYLRESGRVFLTGTQALVRIPLMQ
                     RRLDRAAGLNTAGFISGYRGSPLAAYDLELWRAQKFLEASNVRFLPAVNEDLAATAIL
                     GTQQVEADPDRTVDGVFGIWYGKGPGVDRAGDALKHGNAYGSSPHGGVLVVAGDDHGC
                     VSSSMPHQSDVAFLAWFMPNLNPANVAEYLEYGLYGIALSRFSGMWVGFKAISETVES
                     AMSVQLPSPRQFVKPANFVPPPDGLHIRWPDFPGPQIEERMEAKKAAVLAFAEANPID
                     RKIYDTPNARYGIVTTGKAHLDVMEALRLLGIDRREARRLGVDIYKVGMVWPLAHDSA
                     LDFVHDKHEVLVVEEKRGIIESQFKEYFYDYPGRKPKRMIGKQDEFGHNLVPWIGELS
                     PVSIAGIIASRLDAALPGLDLGARAASLTSGGANLINVTGASRTPYFCSGCPHNTSTR
                     LPEGSKALAGIGCHFMANWMERDTDGLIQMGGEGINWVTRSMFNGDKHVFQNLGDGTF
                     YHSGSIAIRQALAAKTNITYKILFNDAVAMTGGQPVDGPLSVEMVAHNVRAEGVRRIA
                     VVSDQPENFRLDDFPPGTTISHRRDLDTIQRELREIPGVTVIIYAQTCATEKRRRRKR
                     GTMQDPKRFAVINDLVCEGCGDCSVESNCLSVVPKETPFGRKRQIDQNSCNKDFSCVN
                     GFCPSFITVEGGERAAAKPEGFGFPREKLQAIGSPAMPELNGGYDLLVTGVGGTGVIT
                     VGALITMAAHLERKGASVLDFTGFAQKFGPVLSYIRLARTPEQLNQVRIDTERADALI
                     GCDLVVSSSPKASSTYRSGHTRAVLNTAEMSTADFVRHRDANLNARERIDAITRITGS
                     DRLSTVAANELASRLLGNTIYANVLMLGFAWQNGLVPVSLEALLRAIELNGVDIEKNR
                     QAFGWGRLAAADEQYVAALMNEPSAKPAREVETLDSMVERRVGFLQEYQDKALGERYL
                     DVVNRARARESSLRKGHDMPFTAAVAKSWFKTLAYKDEYEVARLHVASGFLESVKREF
                     GDEAKISFHLAPPVLNRAVDARGRPRKRAFGMWMIPLFRLLAAMRRVRGTRFDLLGRT
                     AERRMERALIGEFEATFETLLAHLSADNIDLAAEIIGLYMEIRGYGPVKAEAVAKVRP
                     QIEQKLASFSAARRLAA"
     gene            6337..6798
                     /locus_tag="VFY27_02700"
     CDS             6337..6798
                     /locus_tag="VFY27_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5764448.1"
                     /translation="MLDPKDEALLKALQQDSRLTMQQLGEKVGMSSSACWRRVKSLEE
                     AGVIERYAVMVNPKKAGFSLSSMTLVSLARHEEKNVENFVNEVMRHPEVLECFATSGE
                     ADFHLRVVVENIDAYNRFLDDFIFRLPGVSQVRSNIVLKEIKTDTALPFRD"
     gene            complement(6812..>7899)
                     /locus_tag="VFY27_02705"
     CDS             complement(6812..>7899)
                     /locus_tag="VFY27_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5764449.1"
                     /translation="EVRVGNPARDDVDMGALASIGQRDEVRARVAELSGEANVVCGND
                     DTFELHGADARKGAFFMPTLLYCERPLSAKAVHSVEAFGPVSTVLPYDTLDDAVELAR
                     LGDGSLAGSIVTNDDDVARELVLGTAPYHGRLLVINRHCAGESTGHGSPLPHLVHGGP
                     GRAGGGEELGGIRGVLHYMQRTALQGSPETLSHIGRRWIRGADEKDPGRHPFRKTFEQ
                     LEIGDTLHTAERQITLDDIEHFAEFTGDRFYAHMDEEAAKRNPFFEGRVAHGYLIVSF
                     AAGLFVEPEFGPVLANYGVDDLRFAQPVNYGDSIRVRLTCKQKTLREGTGYGEVRWDT
                     EVTNQDGEVVASYDVLTMVATDEHWQP"
BASE COUNT         1405 a         2494 c         2456 g         1544 t
ORIGIN      
        1 cccggctgga tctcgttcgc ctcgttgccg gtcacgccgt aggtattcgg cacgcccggc
       61 ttcacgctga agtcgtccat ctcgtcgttc aacaggaagc cggcaccttc gaccacgaca
      121 ccgctgccga agcttatgtt cagcgtgtag gtctgcgcga cggcattgcc ccactggtcg
      181 acgatggagt agtgggtcgt gtcgggggac tcgttcgcca ctgtgcctgc cgaaaccatc
      241 gccgagattt catcggtatc gacttcggcc gcgcgctgca tcatgtactc ctcgctcacc
      301 aggcggtcga tgcgggtatc cacgtagtcc gggtcgccca ggtattccgc gcggtccgcg
      361 aagacgcgct tctcgatctc ggcaacgaga tgtatgtatt gcggcgagtt gtgcggcagc
      421 ccctcgaact cgtggttgag gtaatccttc atgctcagca actggatgac cgcaaaaccg
      481 ccggaactcg ggagcggtgc cgagaggatt tcgtatccgc gccactcggc ccggagcggc
      541 gcgcgccaca ccgccttgta cctttcgagg tcctcgagcg ttatcaggcc gtcgccacgc
      601 tccatttcct caacgatcaa ttttgcagtt tcgccctcgt aaaagtccgc cggcccttgt
      661 tccgcgatac gccacagcgt cgccgcaagt tccggctgcc ggaaaacctt ccctgactcg
      721 agatcgccga agtactcgcc gaagttggtc ttgtcgctga accagtcgat catcgttttc
      781 attccgccgc ccagggactc cggcacgacg aagccttctt ctgcgatgcg cgccgcaggc
      841 tttaccaggt cttcccaggg gagcgtgccg tagcgctggt gcgccgccca gaatcccgcg
      901 acggtgccgg gcacgccgac ggccttgtgg ccgatgaggc tggcgccggg aatcacgtcg
      961 ccgttctcgt ccaagtacat gtcgcgatcg gccgcgagcg gcgcggtctc gcggtaatcg
     1021 aggaaggtcg actcgccctt catgtggatg agcatgaaac cgccgccgcc gatgttgccg
     1081 gcttccgggt aggtcaccgc gagcgtgaaa ccggccgcga tagccgcatc cacagcattg
     1141 ccgccgcctt taaggatcgc ctcgctcacc tccgcggcgt agcggtcggg catcgccacc
     1201 gctgcctcgt ccccgccctc gcgattgaca gcttccgctg cggtcgctac ggcttcgggc
     1261 gcttcggttg gcggagagca ggcgaggaca aggagaagag tcgcctcgag cgacaggagc
     1321 ttacagagtc gtaacatcag aatgacccgc tttggaggac gcggcattct tccacagatc
     1381 gggcggattg tcgtcgcgag atgaatctcg ctaccgcatg ctggtccggt accagttgac
     1441 gccgtccgcg tcggtctgga cggccattcg cccctggtag agcaggtagt tcaggtgcgc
     1501 gacagcctca ccggtcgcca tgatcaggtt gttctcgttg atcgggctcc tgaacagcgc
     1561 cgggaaggcg tcaacggcgc gcttcggttc gcgacacaga accgccagtt catccaggca
     1621 atcatgatgt tcgtcgataa gttcctgcag gcgctcgtgc gcgccgcgga atggtttgcc
     1681 gtgggagggc agcaccagta cgtcggccgg gatcctgctt ttcatttccg ccagtgacgt
     1741 tatccagtca tccagcgggt tggcggcagg ctcggtcgga tagacgctga cgttcgacga
     1801 gatcgtcggc aagagctggt cgccggaaat gagcacgttc agcccttcgc agaaaaggca
     1861 ggcatgctcg ggtgaatgtc cgcggccgat gacgacttgc cactcgttcg caccgatgcg
     1921 gacggtgtcg ccgtccttca ggcgccggta actttccggc agggcagcca cgtacttgcc
     1981 gaacatgccg aacattttct ggtagtaaga catcgcctcc ggcggaaaac ccgcggcgtt
     2041 gtagaagcgg atacctgcct cgggcgtcgg tcgccccgta tccgcgacca ggatgcggca
     2101 cagcaggtat tcttcccgcg gcatccacag ttcgacgtcg cgctgctccg tcagccagcc
     2161 ggcacagccg gagtgatccg gatgaaggtg cgtgacaaaa acgcggctga gagggcggtc
     2221 ctgcatcgct tccgagaacg tgttttccca gacgcccctg gtgatatcgg tgaacacacc
     2281 ggtatcgacg atcgcccagg cgccatcatc ttcgagcaac cacaggttga tgtgaccgag
     2341 cgcgaaaggc aggggcatgc gcagccacac cacgcccggc gcaacccgca tggtctcgcc
     2401 ggcgtcaggc ctgtcgtcga actcgtagag aacttcttct cttgcgcgcg ttgctaccat
     2461 gcgggtcctc ttgatgtatg ccgggtattc atgcgcatga tcgcacgaac gtggggtcac
     2521 gcagcgctca tttccgccgc acgatgtccc ggactgcagg agcccgtatc acgggcgagg
     2581 tgcctcgaca agggtcctga cgtcggtcgc ccgtgcgacg ggctcctcca gcgggcgatt
     2641 ccggccctgc cccgtgtttt tacgctgcga gccgccgtgc ggccgagaat gaggcgagct
     2701 tttgttctat ctgcgggcgc acctttgcga cggcctccgc cttgaccggg ccgtagccgc
     2761 gaatctccat gtacaggccg atgatctccg cggccaggtc gatgttgtcg gcgctgagat
     2821 gcgcaaggag agtctcgaac gtggcttcga attcgccgat cagcgcgcgc tccattcgtc
     2881 gttcggccgt gcgcccgagc aggtcgaagc gcgtcccgcg caccctgcgc atggcagcga
     2941 gcagccggaa caggggaatc atccacatgc cgaacgcccg cttgcgaggc ctgccgcgcg
     3001 cgtccacggc cctgttcagg actggcggcg cgaggtggaa actgatcttc gcctcgtcgc
     3061 cgaattcccg ctttaccgat tccaggaagc cgcttgcgac atgcaatctc gccacctcgt
     3121 attcgtcctt gtaggcgagc gtcttgaacc agctcttcgc caccgcggcg gtaaaaggca
     3181 tgtcatgccc cttccgcagc gacgattcgc gtgcccgtgc cctgttgacg acatcgaggt
     3241 agcgttcacc cagcgccttg tcctggtact cctgcaagaa acccacacgc cgctcgacca
     3301 tcgagtcgag cgtttccact tcccgtgccg gcttcgcgga aggttcgttc atcaacgccg
     3361 cgacgtattg ttcgtcggca gcggcaagcc tgccccagcc gaaggcctgc ctgttcttct
     3421 cgatgtcgac gccattcagt tcgattgcgc gcaggagtgc ttccagcgaa accggcacaa
     3481 ggccgttctg ccaggcgaag ccgagcatca gcacgttcgc gtagatcgta ttgcccagca
     3541 gccgtgacgc gagttcgttg gcagcgacag tcgataaccg gtcgctgccg gtgatacggg
     3601 tgatggcgtc aatccgttcc ctggcattga gattcgcatc gcgatgccga acgaaatcgg
     3661 cggtcgacat ttcggcggtg ttcagaaccg cgcgcgtgtg gcccgaccgg taggtactgg
     3721 atgctttcgg cgacgaactt acgacgaggt cacaaccgat aagggcgtct gctctctccg
     3781 tatctatccg gacctggttc agctgctccg gcgtccgcgc cagccgtata tagctcagca
     3841 cagggccgaa cttctgcgca aagccggtaa aatcgagaac gctggcgccc ttgcgttcga
     3901 gatgcgccgc catcgtgatc agcgccccga cggtaatcac gccggtgccg ccgacgcctg
     3961 taaccagcaa atcgtagccg ccattgagtt ccggcatggc gggcgagccg atcgcttgga
     4021 gtttctcccg gggaaaaccg aacccctccg gcttcgccgc agcgcgttca cccccttcga
     4081 cggtgatgaa gctcgggcaa aatccgttga cgcaggaaaa gtccttgttg cagctgttct
     4141 ggtcgatttg ccgcttgcgc ccgaatggcg tttccttcgg caccaccgac aggcaattcg
     4201 actcgacgga gcagtccccg cacccttcgc acaccaggtc gttgatgacg gcgaagcgct
     4261 taggatcttg catcgtgccg cgcttgcgcc ggcgccgctt ttcggttgca caggtctgcg
     4321 catagattat gaccgtgacg cccggaattt cgcgcagctc ccgctggatc gtgtcgaggt
     4381 cgcgccggtg cgatatcgtg gtgccggggg ggaaatcatc cagcctgaag ttctccggct
     4441 ggtcggagac gactgcgatt ctccggacgc cttcggcgcg cacgttgtgc gcgaccatct
     4501 ccacggacag gggcccgtcg accggctgcc cgccggtcat tgcgaccgcg tcattgaaca
     4561 gtatcttgta cgtgatgttc gtcttcgccg cgagcgcctg gcgaatggcg atcgagccgg
     4621 agtgatagaa cgtcccgtcg ccgaggttct ggaacacgtg cttgtcaccg ttgaacatcg
     4681 agcgggtgac ccagttgata ccttcgccgc ccatctggat cagcccatcg gtgtcacgct
     4741 ccatccagtt ggccatgaaa tggcaaccga ttcccgccag cgccttcgat ccttcgggca
     4801 accgggtgga ggtgttgtgc gggcatcctg agcaaaagta cggcgtgcgc gacgcgccgg
     4861 taacattgat caggttggcg ccccccgacg tgaggctcgc ggcgcgcgcg cccaggtcca
     4921 gtcccgggag ggcagcgtcg aggcgtgagg caatgatgcc ggcgatgctc accggggata
     4981 gctcgccgat ccacggcacg aggttgtggc cgaactcatc ctgtttgccg atcatgcgtt
     5041 ttggcttgcg gccgggatag tcgtagaagt attccttgaa ctggctctcg atgatgccgc
     5101 gcttctcttc gacaaccagt acttcgtgct tgtcgtggac gaagtcgaga gcggaatcgt
     5161 gggcgagcgg ccagaccatg ccgactttat agatgtctac tcccaaacgc cgcgcctcgc
     5221 ggcggtcgat gcccagcagg cgcagggctt ccatcacgtc gagatgtgcc tttccggtgg
     5281 tgacgatgcc gtaacgggca ttcggcgtat cgtagatctt gcggtcgatg ggattcgctt
     5341 cggcgaacgc gagcacggcc gcttttttgg cctccatccg ctcctcgatc tgcggacccg
     5401 ggaaatccgg ccagcgaatg tgcaggccgt ccgggggcgg gacgaagttc gcgggcttca
     5461 cgaactgccg gggcgacggc agttgtacgg acattgcgga ctccaccgtc tccgagatcg
     5521 ctttgaaacc gacccacatg ccggagaagc gcgacagcgc gatgccgtac aggccgtatt
     5581 ccaggtattc cgctacgttc gcggggttca ggttgggcat gaaccaggcg aggaaggcaa
     5641 catcggactg gtgcggcatc gaggacgaga cgcatccgtg atcgtcgccg gcgacgacga
     5701 gcacaccgcc gtggggagaa ctgccgtacg cgttgccatg tttgagggcg tcacccgcgc
     5761 ggtcgacccc gggccccttg ccgtaccaga tgccgaagac gccatcgacc gtccggtcgg
     5821 ggtcggcctc cacctgctgc gtcccgagaa tggcggtggc cgccaggtct tcgttgaccg
     5881 cggggaggaa cctgacattg ctcgcctcca ggaatttttg tgcgcgccag agttcgaggt
     5941 cataagccgc aagcggtgac ccgcggtagc cgctgatgaa gcccgcggta ttgagaccgg
     6001 cagcccggtc gaggcgccgt tgcatgagcg ggattcgcac gagggcctgc gtaccggtca
     6061 ggaagaccct gccggattcg cgcaagtacc ggtcgtcgag ttcgtacgag tcgatgcggg
     6121 tcgcttctgc aagcgaagtt gcggtaacca tcggtaaacc ctcgagctgg ctcggccgcg
     6181 gccgaaaagg actgagcggg cccggttagc atcatcgcag aatcgcggcg aaacgggatg
     6241 ccataatagc ccgtgttggg gcatgaaacc ggaattctgt accactttcc ctaggtaaga
     6301 tgggatagaa ttgcctaaaa tcgaagaggc accgaaatgc tggatcccaa agacgaggcg
     6361 ctcctcaagg cactgcagca ggacagccgg ctgaccatgc agcagctcgg cgagaaagtc
     6421 ggcatgtcga gttccgcgtg ctggcggcgc gtgaagagcc tggaggaagc tggcgtgatc
     6481 gaacgttacg ccgtgatggt gaacccgaag aaggcggggt tctcgctgtc gtcgatgacc
     6541 ctggtgtcac tcgcccggca cgaggaaaaa aacgtcgaga acttcgtcaa cgaggtcatg
     6601 cggcatccgg aagtactgga atgcttcgcg accagcggcg aggcggattt ccacctgcgc
     6661 gttgtcgtcg agaacatcga cgcctataac cgatttctgg atgacttcat cttccgcctg
     6721 ccgggggtgt cgcaggtgag atcgaacatc gtcctgaagg aaatcaagac cgatacggcg
     6781 ctgccgtttc gcgattgact tctcggggcg gctacggctg ccagtgttcg tcggtggcga
     6841 ccatcgtcag cacatcgtaa cttgcgacga cttctccatc ctggttcgtc acctccgtgt
     6901 cccagcgcac ctcgccgtag cctgtgccct cgcggagcgt cttctgtttg caggtcagcc
     6961 tgaccctgat cgagtcgccg tagttcactg gttgtgcaaa ccgcaggtca tccacgccat
     7021 agttcgccag taccggaccg aactccggtt ccacgaacag tcccgctgcg aaagacacga
     7081 tcaggtaacc gtgagccacc cgaccttcga agaatgggtt cctcttcgcg gcttcttcgt
     7141 ccatgtgcgc atagaaccgg tcgcctgtga attcggcgaa gtgttcgata tcgtccaacg
     7201 tgatctggcg ctccgcggta tgaagcgtat cgccgatctc aagttgctcg aaagtcttgc
     7261 gaaacgggtg ccggccgggg tctttctcat ccgctccgcg aatccagcgg cgaccaatgt
     7321 ggcttaaggt ttcgggtgaa ccctgcagcg ccgtccgctg catgtaatgg agcacgccac
     7381 gtatgccgcc gagctcctcg ccgccgcccg cccttcccgg tccaccgtga acgaggtgcg
     7441 ggaggggcga accgtgtccg gtcgactcgc cggcacagtg gcgattgatg acgagaagcc
     7501 ggccatgata cggagcagtg cccaggacca gttcacgcgc gacgtcatcg tcgttggtaa
     7561 cgatcgagcc ggcgaggctc ccgtcgccca gccgcgcaag ttcgacggcg tcgtccagcg
     7621 tgtcgtacgg aaggacggta ctgacgggac caaaagcctc gaccgagtgc acggcttttg
     7681 ctgaaagcgg cctctcgcaa taaagaagcg tcggcatgaa gaaggcgcct tttcgcgcgt
     7741 ctgcaccgtg aagctcgaac gtgtcatcgt tgccgcatac gacgtttgct tcgccgctca
     7801 attcggcaac ccgcgcgcga acttcatccc gctggccgat gctggcaagc gctcccatgt
     7861 cgacgtcgtc cctcgcaggg ttgcccacgc gaacctcgc
//
LOCUS       DASUOM010000136         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1282613,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000136 DASUOM010000000
VERSION     DASUOM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282613"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..574
                     /locus_tag="VFY27_02710"
     CDS             194..574
                     /locus_tag="VFY27_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5764450.1"
                     /translation="MHKSRLAGLSIDCNTDDLSAAAGFWSKVLGVPPRPARAGEDANY
                     VPLDMPADEPSVEVQRVDHASRVHLDIESDDIEAEVERLEALGATKVAWVKDWCVMEA
                     PTGQRFCIVPVQSAAFDQKAHEWP"
     gene            complement(585..>2502)
                     /locus_tag="VFY27_02715"
     CDS             complement(585..>2502)
                     /locus_tag="VFY27_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5764451.1"
                     /translation="PRPFMTLIRFDQVSLDFGDQAILHQADFAIDTGERVCLIGRNGA
                     GKTTTLKLITGEVQPEHGEIVRKSELVVSQLAQSLPPGVDASVRQVVASGLSGVRALL
                     NEYETRSQEMASTEAMRELEELHQLIDIHGGWSIDQRIDAILTELDLPGERRMSELSG
                     GWRRRVALATALVQKPDLLLLDEPTNHLDIVTIKWLENIVYSYDGAVLFITHDRAFLQ
                     RLATRIVEIDLGRLESWPGDYANFLQRKEKALEDEAAEHERFDKELQQEEVWIRKGIK
                     ARRTRNEGRARALQRMREERARRVTREQAAQIQIEEADRTGRQVIRAKNVTYRFDDRP
                     LIENFSTRIVRGDRIGILGNNGVGKTTLLRILLGELEPQSGTVKHGTNLETGYFDQLR
                     ESLDPEKSVIDNVAGGRTHVAIGGGERHVIGYLKGFLFSPKRARMPVKALSGGERNRV
                     ILAKLFTRAANLLVLDEPTNDLDMETLEVLEEKLTSFSGTLIVVSHDRQFLDNVVTST
                     IVFEADGGIREYVGGYSDWLRQGKELAEVDNLEPVRATRENAREQTATRSPTKLSYRE
                     QRELENLPKQIEDLESELAALEETVATPDFYSRHNEVVQDTLRLLSEKRSLIDAAIQR
                     WSELEDRQQQLRKR"
BASE COUNT          427 a          775 c          730 g          570 t
ORIGIN      
        1 cgccgctttg attggtgctg attcggaatt ctaaattcga gcgggcttgt tagccaaaac
       61 aaacgtccgc cgaatccgag atgcatacag gcccgatgag aggcaaatct gcctccctcg
      121 ggccttttta tttacgtgaa acgcaaacca ccgcctggta tcgctatttg cgacgcgatt
      181 gccggagttc cacatgcaca agagcaggct ggccggactg agtattgact gcaacactga
      241 cgacctgtcc gccgcggcgg ggttctggag caaggtgctg ggcgttccgc ccaggcccgc
      301 tcgagccggc gaggacgcga actacgttcc cctcgatatg cccgcggacg agccgtccgt
      361 ggaagtgcag cgcgtggatc acgcatcacg ggttcacctc gacatcgaaa gtgacgacat
      421 cgaggcggaa gtagagcggc tagaggcgct cggcgccacc aaagtggctt gggtgaagga
      481 ctggtgcgta atggaggcgc ccacaggaca gcgcttctgc atcgtgccgg ttcaaagcgc
      541 cgcattcgac caaaaggcgc acgagtggcc ctgattacgg atacttaccg tttccgaagt
      601 tgctgctgcc tgtcttcgag ttcgctccag cgttggattg cagcatcgat cagcgatcgt
      661 ttctcgctca gtaatcgtaa agtgtcctgc acgacttcgt tgtggcggct gtagaaatcc
      721 ggtgtcgcta cggtctcttc cagtgctgcc agttcactct cgagatcctc tatttgcttt
      781 ggcaggtttt ccagttcccg ttgttcgcga taggaaagct tcgtcgggga acgcgtagcg
      841 gtttgttctc gggcgttctc ccgtgtcgcg cggaccggct cgagattgtc cacctctgcg
      901 agttccttgc cctggcgtag ccagtcgctg taaccgccga catattcccg tatgcccccg
      961 tcagcctcga agacgatcgt gctggtgacg acgttgtcca ggaactgccg gtcatggctc
     1021 acgacgatca gtgtcccgct gaagctcgtc aacttttctt caaggacctc cagcgtctcc
     1081 atgtccagat cgttggtcgg ttcgtccagt accagcaggt tcgccgctcg tgtgaacagt
     1141 tttgcgagga tcactcggtt gcgctcgccg ccggataagg ccttgaccgg catccgtgca
     1201 cgcttggggg aaaagaggaa tcctttcagg taaccgatga catgccgctc accgccgccg
     1261 atcgcgacgt gcgtgcgtcc gcccgcgacg ttgtcgatta cggacttctc cggatccagg
     1321 gactcgcgaa gctggtcgaa atatcctgtc tccaggttgg ttccgtgctt cacggttccc
     1381 gactgcggct ctaattctcc caggaggatc ctgagcaggg tcgttttgcc gacaccattg
     1441 ttgcccagga taccgatccg gtccccgcgc acgatccggg tcgaaaagtt ctctatcaat
     1501 ggtctgtcat cgaagcggta agtgacgttc ttcgcgcgga ttacctgccg cccggtgcga
     1561 tccgcttcct cgatctggat ctgtgccgcc tgttcacgcg tgacccgccg ggcacgttct
     1621 tcgcgcattc tctgcagtgc gcgggcgcgc ccttcgttgc gcgtgcggcg tgcctttatg
     1681 cccttgcgta tccacacttc ttcctgctgc agctctttgt cgaaacgctc gtgttccgcc
     1741 gcttcgtcct ccagtgcttt ttccttgcgc tgcaggaagt ttgcgtagtc gccgggccag
     1801 ctttcgagcc ggccaagatc gatctcgacg atgcgcgtcg ccagccgctg caggaaggct
     1861 cgatcatggg tgatgaacag caccgcgccg tcgtagctgt acacgatatt ctcgagccac
     1921 ttgatggtga cgatgtccag gtggttcgtt ggctcgtcca ggagcagcag gtccggtttc
     1981 tgcaccagcg ccgttgccag cgcgacacgc cggcgccagc ctcccgatag ctcgctcatc
     2041 ctgcgctccc cgggaaggtc cagttctgtg aggattgcat cgatgcgctg gtcgatgctc
     2101 cagccgccat ggatgtcgat cagctggtgg agttcctcga gctcacgcat cgcctccgtg
     2161 ctcgccattt cctgcgaccg tgtttcgtac tcgttcagca gcgcccgcac accggacagg
     2221 ccggaagcca ctacctgccg gacgctggca tccacgccgg gcggaagcga ctgcgccagc
     2281 tggctcacca ccagttcgct tttccgcacg atctcgccat gctccggctg cacctcacct
     2341 gtgatgagct tgagtgtcgt tgtcttgccc gcgccattgc ggccgatcag gcagacacgc
     2401 tcgcctgtat cgattgcaaa atctgcctgg tgcaggattg cctggtcgcc gaagtcgagc
     2461 gaaacctggt cgaagcggat aagagtcata aacggtcgtg ga
//
LOCUS       DASUOM010000137         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_269905,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000137 DASUOM010000000
VERSION     DASUOM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269905"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFY27_02720"
     CDS             complement(<1..421)
                     /locus_tag="VFY27_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX5764452.1"
                     /translation="MPKKAKTERKGGSGRIYKHPVPGRGALLDHLRDAGRPQTIEVLM
                     QDLGLKGQRTREALLDTLDRMVRAGQIIRNRRDEYCLTEKLELVAGMVSGHRDGFGFV
                     IREDGEDDVYLSAREMRSLIDGDRVAIRVVGRDYRGRP"
     gene            526..612
                     /locus_tag="VFY27_02725"
     tRNA            526..612
                     /locus_tag="VFY27_02725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Leu,seq:cag)
     gene            complement(774..1721)
                     /locus_tag="VFY27_02730"
     CDS             complement(774..1721)
                     /locus_tag="VFY27_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5764453.1"
                     /translation="MIYTFTKPLGAAVAGFLVSLIVASPVILHLRQIGYTTMKTLNNP
                     LHACSLGALLLGSISLAAVFAPTDVLAQADCDSLPPHSTLQSTLDSIVTGGGNGGFGN
                     DMWATVVNRDSQVCAVAFSSDDRAGQWPGSRVISAQKASTANAFSLPGFYPNVGLGRA
                     LASGNLYGLVIPGGSLYGLQHSNPVDTAVAYGNRNHSGGNPGRYGQPNDPLVGAFVGG
                     VNVFGGGLALYDASGQLVGALGVSGDTSCTDHIIAWKVRDALELDNIPGGVGDGSTTD
                     NLILTDPVTPNTFQHPICGFDEDNIIPTLATDFPIGVNP"
     gene            2091..>2317
                     /locus_tag="VFY27_02735"
     CDS             2091..>2317
                     /locus_tag="VFY27_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5764454.1"
                     /translation="MKVREIMTQDPAGCAPEANIRDVARMMAENDCGEIPVLDESSRP
                     IGVVTDRDIACRVVAQGKDPGQTSARDVMSSP"
BASE COUNT          450 a          698 c          723 g          446 t
ORIGIN      
        1 ccggacgccc gcggtagtcg cggccgacga ccctgatcgc cacgcgatca ccgtcgatca
       61 gcgagcgcat ctcccgtgcg gagagataga catcgtcctc gccatcctcg cggatgacga
      121 aaccgaatcc gtcgcggtga ccgctgacca tgcccgcgac cagttcgagt ttctcagtca
      181 ggcaatactc atcacgccgg ttccggatga tttgcccggc tcgaaccatt cgatcgagcg
      241 tatcgagcag ggcctcccgc gtgcgctggc ctttcagccc gaggtcctgc atcaggacct
      301 caatggtctg gggccggcct gcatcgcgca ggtggtccag cagcgcgccg cgcccaggaa
      361 ccgggtgctt ataaatgcgg ccgctgccgc ctttcctttc ggtttttgct tttttcggca
      421 aaggttctcg ctctgccgcg ggaaggcgcg gcgaattgac agggtcgggc ggctttgagt
      481 acagtgcgcc acccgcaaag acccgaattg taccggtctt gacctgccga ggtggcggaa
      541 ttggtagacg cgctagcttc aggtgctagt gggggtaacc ccgtggaggt tcaagtcctc
      601 ttctcggcac caaccctgtc agaagcgctg caacccgcat ggacctagct tcttcccctt
      661 agggcgggag aataacagcg gaagacccgg ggccatggtc ctgcgtcttg acgcatcccg
      721 gcgggcttga gaccgccggg gtgaatcaaa actcctgccg atcgcttgct cctttagggg
      781 ttgactccga tcgggaaatc ggtggcgagc gtagggatga tgttgtcctc gtcgaagccg
      841 cagatcgggt gctggaaggt attcggcgtg acgggatcgg tcaggatcag attgtcggtc
      901 gtgctgccat caccgacgcc gccgggaatg ttgtccagct ccagcgcgtc acgcactttc
      961 caggcgatga tgtgatcggt gcaggacgtg tcgccgctga cgcctagtgc cccaaccagt
     1021 tggccgctcg catcgtaaag cgcgagccca ccgccgaata cgttgacgcc gccgacaaaa
     1081 gcgcccacca gcggatcgtt tggctggcca tatcttccgg gattgccgcc ggagtgattt
     1141 cggttgccgt aagcgacggc tgtatccacg ggattgctgt gctgcaatcc atagaggctg
     1201 ccacccggga tcaccagtcc gtacagattg ccgctcgcga gcgcacgacc cagacctacg
     1261 ttcgggtaaa agcccggcag gctgaaggca ttggcggtac tggccttttg tgccgagatc
     1321 acgcggctgc ctggccactg gccggcccgg tcatcgctgg agaaggcgac ggcgcagacc
     1381 tgactgtcgc gattgaccac ggtggcccac atgtcgttgc cgaaaccgcc gttgcccccg
     1441 ccggtcacga tactatccag cgtgctttgc agcgtactgt gtggaggaag actatcgcag
     1501 tcggcctggg caaggacgtc ggtcggtgca aacaccgccg ccagcgagat actccccagc
     1561 aggagagcgc cgagggagca agcatggagt ggattattga gcgttttcat agttgtgtac
     1621 ccaatttgtc ggagatggag gatgacgggc gatgccacaa tcaacgagac taggaagccc
     1681 gcgacagcag ccccgagagg cttggtaaaa gtgtagatca tcccgcagga aacgtagtgt
     1741 cagcggacga cgataaaaaa caccatcctt atagctcccc tccgaagtga ctaacgctcg
     1801 ccgctgcatt accaaagatg ctctgcgcct gggcgcgacc ttcgtgccag tgcgatgtcg
     1861 ggtttgcagg gccgcagata aactttacaa atggggctcc tgctcctcca ggctgtcgcg
     1921 tccctgatcc ggcccacagg cggtctgttg cgcttgatct ccccttggct ttccctggct
     1981 ctcgtcaccg aatgtgctgg catagttgtt gcagccagag tcggttgcaa cttgtgcaac
     2041 tggtccactg ttgtttcccc aggtaagacc aagcgaaacc ggaggatgaa atgaaagtac
     2101 gagaaatcat gacccaggac ccagccggct gcgcgcctga agccaacatc agggatgtcg
     2161 ccaggatgat ggcggagaac gattgcggcg aaatcccggt gctcgatgag agcagccggc
     2221 cgatcggcgt cgtgaccgac agggacatcg cgtgccgcgt ggtggcgcaa ggaaaggacc
     2281 ccggccagac ttcggcgcgg gacgtaatgt cgagccc
//
LOCUS       DASUOM010000138         7186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1266399,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000138 DASUOM010000000
VERSION     DASUOM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266399"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..643)
                     /locus_tag="VFY27_02740"
     CDS             complement(191..643)
                     /locus_tag="VFY27_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764455.1"
                     /translation="MPNTLLTDFQSACRAAHEQVQMLQDLYEHLCGPQSHFAAASIDV
                     DDRLSELRHFREVLSQKLKQHDLLPTQPDPERESLLELITEVKATVTGEEKPAADERL
                     ASEESELLQHIEKMLEHDDDAALEEARGRTRDAIARLSNPAAGTDGEA"
     gene            complement(646..1530)
                     /locus_tag="VFY27_02745"
     CDS             complement(646..1530)
                     /locus_tag="VFY27_02745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HEX5764456.1"
                     /translation="MKKTDGATLLLVNPNARSGNEQLGDLLLPLQDIGAVIAPKSLGR
                     EAVGKAIARHAGSVARVVIGGGDGSLHAALPAVLEAKLPLGVLPLGTANDFARSLGIA
                     SVSAAVETIVSGFTRPVDVGIVNGIYFLNAVGIGLGPEINRNLDKESKSSLGVLAYLV
                     HAIRNLRRSRGMSAVIECDGSLHALKSLQITIGNGIHYGGGMTIARDARLDDGILDVL
                     SIPPQGLFSLLLKGPAIRSGDAGKIDELQTFRASEVRVRTRRPRDVTADGEQVTRTPI
                     ECRVLPGRLQVFAPSTRE"
     gene            complement(1594..2427)
                     /locus_tag="VFY27_02750"
     CDS             complement(1594..2427)
                     /locus_tag="VFY27_02750"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX5764457.1"
                     /translation="MTTMGSAGSSRRYSRNLHFIARAGYAARGIIYLIVGGLAALAAL
                     GRGGGTTDSRGALSQVMSAPFGKFLLIAIALGLLGYAAWRLVQALLDPDHHGTEAKGI
                     VIRAALAVSAVIHIGLAFFAASLALGWGTSQESGSGDSSSREWTAWLLSQPAGQWLVA
                     GVGAAIVGAGLAHIVKGWRADFERHFQMTASEREFITPVSRFGLCSRGVAFLIIGGFL
                     FIAAIQHDPSEARGLSGALQALQQQPFGWILLAILALGLFAFGAYSIIESVYRRVDVP
                     A"
     gene            2651..5053
                     /locus_tag="VFY27_02755"
     CDS             2651..5053
                     /locus_tag="VFY27_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764458.1"
                     /translation="MFAKNYNEGHQMFRRKAISRAVLVALSTAPVPLVMAAELEEIVV
                     TATKREENLQDVAVSVQTLGEQQLEQLNIANFDDYIRYLPNVNAAGRGPGQSSIFIRG
                     MATDSSDQTSVEIGAPVPNVALYLDEQPVSSGGRNLDVYAADLARVEVLPGPQGTLFG
                     ASSQGGTVRLITNKPVYNELLGGLDASVATTHKGEQSNSVEGYVNFPVIEDKLAVRAV
                     FYNAVEGGYIDNVFGENSYTAEDVGFPAGAESVVVNNLPLVEEDFNDATYTGTRVSAK
                     YAINDEWELTGQYMQQKLNVDGVFDHAPPSIAEFDDQQSGQRVVGDLQVQRFFPDMLE
                     DEFSQYALTVTGRLRELDLVYVGSYLDREVNNSFDYSGYTEVGGFGYYYLCQPTYTVC
                     GDPTQAMIAFIENDRTTHELRIASSENERLNFVAGLFFDDIETGVDTNFYVAGSTGFF
                     APNVPHSQATHFNPNPRAPGITFMNDAIRTEEQFAAFGELTYDLTDEWSVTVGARYYD
                     IESALVGSSNFATLGDVDGDGGISFDEIFADDLPLKEDDTILKGSVTYHLGDDSLFFV
                     TYAEGFRPGGFNRTDSPEVPKTYISDEVTNLELGWKTTLLDGAMRFNGSIYRIDWEGL
                     QVGITDFNISVITFTTNAADAEILGFEGDLTWAATDNLTLAAAWSFNDTEMTNVPQGV
                     TDIAGEGSELALAPKIQYNLRGRYQWAMGDRDPYAQVVYAYTDDQFSSIVVHNRFPQD
                     SYNTVDAALGIDMENFTVELFGENLTDERAELFVNSLDTDLRITTNRPRTLGVRVSWD
                     FQ"
     gene            5098..7104
                     /locus_tag="VFY27_02760"
     CDS             5098..7104
                     /locus_tag="VFY27_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5764459.1"
                     /translation="MSEPATPVPETGAALARARQLLEGGKLDEALSSARAAADADPSD
                     PEPLYLKAVAQRYLRQPAAALATLGELLTLEPRFARAYQEQGHLYKLLGKVPQALDAY
                     QRAVELNRGLIASWRELAELNALKGAKAAHAVAEAEYRRLASLPPELLSVTSLIQEGK
                     LFQAETLCRKFLRAHPHHIEAMRLLARLGMKLLIYDDAEFLLESCVEFAPDYWLARSD
                     YVEVLHRRQKFRQALAQAELLRDRFPGNPAFELSYANECVAVGEFQAALEVYDRLIAA
                     RAERDQAHRARGHVLKTVGRVEEAIGAYRNAYRARPDFGDAYWSLANLKTYRFTDDEI
                     ASMQQLVDRPETSTADRFHLLFALGKAFEDRADPARAFGYYSDGNRLRRQHLRYDPDR
                     AEAAMRAQAEVCTASLFERRAGAGADNPDPIFIVGLPRSGSTLLEQILASHSQVDGTM
                     ELPNIIALAHRLNGRRMISDEARYPKILAELSHDELKAAGTAYIEDTRFHRAGAPRFI
                     DKMPNNFMHVGLIHMILPHAKIIDARRHPVASCFSNFKQLFADGQEFTYDLDEMARYY
                     RAYTALMDHWDRVLPGKVLRIHYESVVEDLEGQLRRLLDFLELPFEPQCLDFHRTGRS
                     VRTPSSEQVRQPIFRRGVEQWRNFEPYLVNVRERLGDLVDSYPA"
BASE COUNT         1417 a         2281 c         2181 g         1307 t
ORIGIN      
        1 ggcaacagcc tggtgaccga cggaccgttc gtcgagacaa aggaataccg cgtcagcagc
       61 tgacgcagtc gcgggcgagt tcgccgaccc atattcgcac tcgcccgcat tcgccgttcg
      121 taaggcaacc cggtcgatat cgtcgccggt tccattcggc gtgggctcct gcaggtggca
      181 ggaaggccgg ctacgcttcg ccgtcggtcc cggcggccgg gttagagagc cgcgcgatcg
      241 catctcgcgt gcggccgcga gcctcctcga gagcagcgtc gtcgtcgtgc tcgagcattt
      301 tctcgatgtg ctgcaacagc tcgctttcct cgctcgccag gcgctcatcc gctgcgggct
      361 tttcctcgcc ggttacggtc gccttcacct ctgtgatcaa ctccagcagg ctttcccgtt
      421 cgggatcggg ttgcgtcggc agcaggtcgt gctgcttcag tttctgcgat agcacttccc
      481 ggaagtggcg cagctcggac aggcgatcat ccacatcgat cgaggcagca gcgaagtggc
      541 tctgggggcc acaaagatgc tcgtacaaat cctgaagcat ctgtacctgc tcatgcgcgg
      601 cgcggcatgc gctctggaaa tcggtaagca aggtgttggg catcgttact ccctcgtgct
      661 cggtgcgaat acctgcagcc tgccgggcag gacccggcat tcgatcggcg tccgggtcac
      721 ctgctcgcca tccgcagtga catctcgcgg cctccgagtg cgcacccgga cttcgctcgc
      781 gcgaaaggtc tgcagttcgt cgattttacc ggcgtccccg gagcgaatgg caggaccttt
      841 cagcaacagg ctaaagagac cctgcggcgg gatcgaaagc acatccagta tcccgtcatc
      901 gagccgcgcg tccctggcga tggtcatgcc gccgccgtaa tgaatgccgt tgccgatcgt
      961 tatctgcagg ctcttaagag catgaaggga accatcgcac tcgataacgg cggacatacc
     1021 gcggctccgc cgcaggttcc tgatggcgtg taccaggtag gcgagaacac caagactgct
     1081 cttgctttcc ttgtcaaggt tcctgttgat ctcgggtcca agaccgattc cgactgcatt
     1141 caggaagtaa atgccattga cgattccgac atcgaccggc cttgtaaatc cgctgacaat
     1201 ggtctcgacc gcggcagaaa cactggcaat cccgagcgat cgggcgaaat cgtttgctgt
     1261 ccccagcggc aacacgccaa gcggcagttt cgcctcaagg accgccggca acgccgcatg
     1321 caggcttccg tcgccaccgc caatcacaac acgggcgaca gaacctgcat gacgcgcgat
     1381 cgctttcccc acggcttcgc ggcccaggct cttcggagca attaccgcgc ctatatcctg
     1441 caagggcagg agcaaatctc ccagttgctc gttgccgcta cgggcattcg gattgaccag
     1501 caacagcgtc gcaccatcag tcttcttcat tggttccgcc ccgggtccag caagccgtca
     1561 aggggcagca gccatgcagc cttcgccacc gggtcacgcg ggaacatcga ctcttctgta
     1621 caccgactcg atgatgctat aggcgccgaa tgcgaatagc cccaaagcaa ggatcgccag
     1681 caagatccag ccgaacggct gctgttgaag cgcctgaagc gcaccgctga gcccgcgcgc
     1741 ttcgctggga tcatgctgga ttgccgcgat gaacaggaat cctccgataa tgagaaacgc
     1801 gacgccgcgc gagcagaggc cgaacctgct gaccggagtt atgaattccc gctcgctcgc
     1861 ggtcatctgg aaatgccgtt cgaaatcagc tcgccatcct ttgacgatat gcgcaaggcc
     1921 cgccccgaca atggccgcgc ccaccccggc taccagccat tgtcccgccg gttggctcag
     1981 gagccacgcc gtccattcgc ggctggaact gtccccggac ccgctttcct ggcttgtgcc
     2041 ccagcctaag gcgaggctcg cggcaaaaaa agcgaggccg atgtggatca cggcactaac
     2101 tgcgagagct gcgcgtatga caatgccttt cgcttccgta ccgtgatggt ccggatcgag
     2161 aagtgcctgt acgaggcgcc acgcggcata acccagcagg ccgagagcta tcgcaatcag
     2221 caggaacttg ccgaaaggcg cgctcatcac ctggctcaaa gccccgcggc tgtctgtcgt
     2281 cccgccgccg cggccgagcg ccgcaagcgc ggcaagtccg ccgacgatca ggtaaatgat
     2341 gccccgggcg gcgtatcccg ccctggcgat gaaatgcaga ttgcgcgaat agcggcgcga
     2401 cgatccggcg gaacccatcg ttgtcattga tattcctctc caaccgccga atcagcgtgc
     2461 aaaggttgtg ccaaacagac gttatggacg atgacaggcg gcgcctcgtc tgggtaaccg
     2521 gggaaaggac tgcttacccg tgtagagggc gagctgcatg gcgacacatg accgtcacag
     2581 gggcttgatc tatactgctg gcgccggtgc ttagcgggag ttgaccgagg gactgcacgt
     2641 gttccggagc atgttcgcca agaactataa cgagggccac cagatgtttc gcaggaaggc
     2701 aataagtcgg gcagtgctgg tcgcattgtc tacggcgccg gtgccgctgg tgatggcggc
     2761 ggaactggaa gaaatcgtgg tgaccgccac gaagcgggaa gagaacctgc aggacgtggc
     2821 agtttccgtc cagacgctcg gcgagcaaca gctcgaacag ctcaatatcg caaacttcga
     2881 cgactacatc cgctacctgc cgaacgtcaa cgccgcaggc cgcgggcctg gtcagtcgtc
     2941 catcttcatt cgaggcatgg cgaccgattc atccgaccaa acgtcggtcg agatcggtgc
     3001 accggtaccg aacgtcgccc tctacctgga tgagcagccg gtcagctccg gcgggcgcaa
     3061 cctcgacgtc tacgccgccg acctggcccg cgtcgaggtg ctgccgggcc cgcaaggtac
     3121 cctgttcggg gcgagttccc agggcggtac ggtgaggctg atcaccaaca agccggtcta
     3181 caacgaactg ctgggcgggc tggacgcaag cgtcgccacc actcacaagg gcgagcaaag
     3241 caacagcgtg gaaggctacg tcaactttcc cgtcatcgaa gacaagctgg ccgtgcgcgc
     3301 ggtgttctac aacgcggtcg agggaggcta catcgacaat gtgttcggcg agaactccta
     3361 tactgccgag gatgtcgggt ttcccgcggg cgccgaatcg gtcgtggtga ataacctgcc
     3421 cctggtcgag gaagatttca acgacgcgac ctacacgggc acccgtgtca gcgcgaagta
     3481 tgcgatcaac gacgaatggg agctgaccgg ccagtacatg cagcagaaac tgaacgtcga
     3541 cggcgtattc gaccatgcgc cgccgagcat cgccgaattc gacgaccagc agtccggcca
     3601 gcgcgtcgtg ggcgacctgc aggtgcaacg cttcttcccc gacatgctgg aggacgagtt
     3661 ctcccagtac gcactgaccg tgaccggccg cctgcgcgaa ctcgacctgg tatacgtcgg
     3721 ttcctatctc gaccgcgagg tgaacaacag cttcgactat agcggctaca ccgaggtcgg
     3781 cggcttcggc tactactacc tgtgccagcc cacctacacg gtttgcggcg atcctacgca
     3841 ggcgatgatc gcgttcatcg aaaacgaccg gacgacgcat gaacttcgta tcgcctcgtc
     3901 ggaaaacgag cgcctgaact tcgtggcagg tctcttcttc gacgacatcg aaaccggcgt
     3961 cgatacgaat ttctacgtag ccggctcgac cgggttcttc gcgcccaacg tgccgcactc
     4021 ccaggcaact catttcaacc cgaacccgcg ggcgcccggc atcaccttca tgaacgacgc
     4081 cattcgcacg gaagagcagt tcgcggcatt cggcgagctc acttacgacc tgacggatga
     4141 gtggtcggta acggtcggcg cacgctacta cgatatcgaa tccgcgcttg tagggtcttc
     4201 gaatttcgcc actctgggcg acgtggacgg agacggcggc atcagtttcg acgagatctt
     4261 cgccgacgat cttccgttga aggaagacga cacgatcctg aagggcagcg tcacgtatca
     4321 cctcggcgac gattcgctgt ttttcgtcac ttacgcggaa ggcttccgcc cgggcggctt
     4381 caatcgtacc gacagtcccg aggtgccgaa gacgtacatc agtgatgagg tcaccaacct
     4441 ggagctgggc tggaagacta ccctgctgga tggcgcgatg cgtttcaacg ggtcgattta
     4501 caggatcgac tgggaaggac tgcaggtcgg catcaccgat ttcaacattt ccgtcataac
     4561 cttcaccacc aacgccgcgg acgccgagat attgggcttc gagggtgacc tgacgtgggc
     4621 cgcgaccgac aacctgaccc tggcagcggc atggtccttc aacgatacgg agatgacgaa
     4681 cgtaccacag ggcgtcacgg acattgccgg cgaaggttcg gaactcgcgc ttgcgccgaa
     4741 gatccagtac aacctgaggg gacgctacca gtgggccatg ggcgaccgcg atccttacgc
     4801 gcaggttgtg tacgcataca cggacgacca gttcagctcc atcgtcgtac acaaccgttt
     4861 tccgcaggac tcctacaaca ccgtagacgc ggccctgggc atcgacatgg agaacttcac
     4921 ggtcgagctg ttcggtgaga acctgacgga cgagcgcgcg gaactgttcg tcaattccct
     4981 ggacacggac ctgcgcatca ctaccaatcg tccccgcacg ctgggggtgc gggtatcgtg
     5041 ggacttccag taaccctgcc agcggaatga atacccgtag cggaagctgc tgccgcggtg
     5101 tctgaacccg caacgcccgt gccggaaacc ggggccgccc tggcccgcgc aagacagctc
     5161 ctggaaggcg gcaaactgga cgaggcttta tcgtcggcac gggcagcggc tgacgccgac
     5221 ccttccgatc ccgaaccgct ttacctgaag gccgtggcgc agcgctacct ccgccagccc
     5281 gcggctgcac tggcaacgct cggtgagtta ctgacgctgg agccgcgctt tgcccgtgcg
     5341 taccaggaac agggccacct gtacaaactg ctgggtaaag tgccgcaggc gcttgacgcc
     5401 taccagcgcg cggtggagct gaaccgcggc ctcatcgcat cctggcgcga actggcggaa
     5461 ctcaatgcgc taaagggtgc gaaggccgcg cacgccgtgg cagaagccga gtaccgccgg
     5521 ctcgcgtcgc tcccgccgga actcttgagc gtcaccagcc tgatacagga gggcaagctg
     5581 ttccaggcgg agactctttg ccggaagttc ctgcgcgcgc atccgcacca catcgaagcg
     5641 atgcgattgc tggcgcgtct cggcatgaag ctcctgatct atgacgacgc ggagttcctg
     5701 ctggaaagtt gcgtggagtt cgctccggat tactggctcg cgcggagcga ttacgtagaa
     5761 gtactgcacc ggcggcagaa gttcaggcag gcgcttgcgc aggcggaatt gttgcgcgac
     5821 cgcttccccg ggaatcctgc tttcgagctc tcctacgcga acgaatgcgt ggcggtcgga
     5881 gaattccagg cggcgctcga ggtttacgac cggctgattg cggcccgcgc ggagcgcgac
     5941 caggcgcatc gggcacgtgg tcatgtcctg aagaccgttg gtcgcgtaga ggaggccatt
     6001 ggtgcgtatc gcaatgccta ccgggcgcgc cccgacttcg gcgatgccta ctggagcctc
     6061 gcgaacctga agacctatcg cttcacggac gacgagatcg cgtcgatgca gcaactggtt
     6121 gaccgtccgg agacatccac tgccgaccga ttccacctgc tcttcgccct gggcaaggcg
     6181 ttcgaggatc gcgcggaccc ggcccgtgct ttcggctact acagtgatgg caatcgcttg
     6241 cgcaggcagc acctgcgcta cgaccctgac cgtgcggaag ccgcgatgcg ggcacaggcg
     6301 gaggtttgca ccgcatcgtt gttcgagagg cgggcgggcg ccggcgcgga caatccggac
     6361 cccatcttca tcgtcgggct cccgcgctcc ggctcgacgc tgctggaaca gattctggct
     6421 tcgcactcgc aggtcgacgg gacgatggag ctgccgaaca tcatcgccct tgcgcatcgc
     6481 ctgaacggcc gccggatgat ttcggacgag gcgcgctatc ctaagatcct ggcagagctt
     6541 tcacacgacg aattgaaagc tgccggcact gcgtacatcg aggacacccg ctttcatcgc
     6601 gcaggcgcac cgcgtttcat cgacaagatg ccgaacaact tcatgcacgt cggcctgatc
     6661 cacatgatcc tgccgcatgc gaaaatcatc gacgcgcgcc gccatcccgt ggcgagctgc
     6721 ttcagtaatt tcaagcagct gttcgccgac ggccaggaat tcacctacga cctcgacgag
     6781 atggcccgtt actaccgcgc gtacacggcg ctgatggacc actgggaccg ggtgctgccg
     6841 ggcaaggtcc tgcgcatcca ctatgaatcc gtagtggagg atctcgaagg gcagttgcga
     6901 aggttgctcg acttccttga gcttccgttc gagccgcagt gcctcgactt tcaccgcacc
     6961 gggcgatcag tgcggacgcc gagttccgaa caggtgcggc agcctatttt ccggcgcggc
     7021 gtggagcagt ggcggaactt tgaaccgtac ctcgtcaatg tgagggagcg gcttggcgac
     7081 ctggtggatt cgtaccctgc ttgacctgcg ccacgggcca aaccagtatg ctgagcccgt
     7141 cctgaatcac tgttgctccg gtacggggcg cgaatctgcg acaccg
//
LOCUS       DASUOM010000139         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1334710,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000139 DASUOM010000000
VERSION     DASUOM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334710"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFY27_02765"
     CDS             complement(<1..224)
                     /locus_tag="VFY27_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764460.1"
                     /translation="MSVSRLENISRIGVEQMGSLADSLHDPDLLRLENLDTNMRPPPA
                     AVAFTKRAIDDDDANSYLPFIGLNSVREAAT"
     gene            complement(248..673)
                     /locus_tag="VFY27_02770"
     CDS             complement(248..673)
                     /locus_tag="VFY27_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX5764461.1"
                     /translation="MEVAPVDILYPVLAMFALTAFCLFRLARLRFMAVRHGEIDPKFF
                     KLYRDGDEPPTLRVHARHLTNLFEAPVLFYVIVIIAFVTRQAGALPLVLAWAYVILRY
                     AHSYIHLTSNRVLTRFRVFAVSWLVLVVLWLVVFAGLVL"
     gene            856..1794
                     /locus_tag="VFY27_02775"
     CDS             856..1794
                     /locus_tag="VFY27_02775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5764462.1"
                     /translation="MLPRKIRANVRGVATFAALTTNTILWFVPLMLLAMVKLVLPVSR
                     ARVTITRWLMALGENWVSGNALILGLRDDARLDARGVNDLNRRSWYLVIANHQTWSDI
                     LILQTVFNRRIPFLKFFIKQELIWFPLLGMAWWALDMPFMKRYSKSYLAKHPEKRGDD
                     LKATRQACRKFSATPTSVINFVEGTRFSLEKKERRGSTYNNLLPPRAGGIALALSSMG
                     SMFDAILDVTVVYPGEVITFWDLCCGDQRPVIVDVVSRPVEERLLQGDYANDREYRST
                     IHRWLTGIWSEKDARIDALRADAGQREPRAADADLA"
     gene            1831..2136
                     /locus_tag="VFY27_02780"
     CDS             1831..2136
                     /locus_tag="VFY27_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5764463.1"
                     /translation="MSNNRNWSVYMLRCGDGSLYTGIATDVKRRLEEHRAEEGAKYLR
                     GRGPLEVVFQSEVGSRSLATRAELRIKRLSRSAKEEFIAARGKSDLLADLQPQEGVC"
     gene            complement(2158..>3123)
                     /locus_tag="VFY27_02785"
     CDS             complement(2158..>3123)
                     /locus_tag="VFY27_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764464.1"
                     /translation="LLGIAVAWYVAWRWQERPEVAQFDWPVASSTSESAERVTVTWLG
                     ITTLLFDDGETQLLVDGTFSRPGLFDLLSQRRVHSDAATINAALAEFRINRLAAIIPV
                     HSHFDHAMDAGFVANRTQAVVLGSESTANIARGADVPVSQYQILADGETRQFGRFTIK
                     LIESRHAPIGFGNGPPFSGTIDKPLTQPAPIRHWREGGTWSVLISHPKGTALVQGSGG
                     FVEGALAGETADVVMLGVAGLASLGPRYVGEYWRETVVSTKATRVFPVHYEDFTQPFG
                     EVRLFPDIVDDLPKTAKWLNELAADRRHPVSISTLPFGQTVAIYP"
BASE COUNT          645 a          913 c          977 g          588 t
ORIGIN      
        1 gtagctgcct cccggacgga gttgagcccg ataaacggca ggtagctgtt ggcgtcgtcg
       61 tcgtcgatcg cgcgcttcgt gaaagcgacg gcggcaggcg gaggtcgcat gttcgtgtcg
      121 agattctcga ggcgaagcaa atccggatcg tgcaggctgt cagcaaggct gcccatctgc
      181 tcgaccccga tcctgctgat gttttccagg cgtgaaaccg acatcggccg ctcccctttg
      241 ccggcggtta aaggaccagg cctgcgaaaa cgaccagcca cagcaccacg aggaccagcc
      301 agctcacggc gaagacgcgg aatcgcgtca acacccggtt ggaggtgagg tgaatatagc
      361 tgtgagcgta cctcaggatg acgtaggccc atgccagcac cagcggcagc gcacctgcct
      421 gccgtgtgac aaaggcgatg atcacgatga cgtaaaagag caccggagcc tcgaacaggt
      481 tcgtgaggtg cctggcatga acccggaggg tggggggctc gtcgccgtcg cggtacagct
      541 tgaagaattt ggggtcgatc tcgccgtgac ggacggccat gaaccgcaac cgggcaaggc
      601 gaaaaaggca aaacgccgtt agcgcgaaca tcgccagtac agggtagagt atgtccactg
      661 gagcgacttc cataacgcag cctccgggct cgtgttgtaa ttctttgatg cgacggcgca
      721 ggtctgcctg caggtccgcg cagcatagcc gacttttctg cgcaggccca aaaaacgatg
      781 cagctggctc gaatcgatta tcgaggacct gttgcactag aataacgaac gtcgcacgca
      841 ccggtacgag gcgagatgct gccgagaaaa ataagagcaa acgtgcgcgg cgttgccacg
      901 tttgccgcgc tcactaccaa tacgatcctc tggttcgtgc cgctgatgtt gcttgcgatg
      961 gtgaaactgg ttctgccggt aagccgggca agggtgacga tcacccgttg gctgatggcg
     1021 ctcggcgaga actgggttag cggcaacgcc ctgattctcg gcctgagaga tgacgcacgc
     1081 ctcgatgccc gtggcgtaaa cgacctcaat cgacgcagtt ggtacctggt gattgcaaat
     1141 caccagacct ggtccgacat cctgatcctg cagaccgttt tcaatcgccg catcccgttc
     1201 ctgaagtttt tcatcaagca ggaattgatc tggtttccgc ttctggggat ggcctggtgg
     1261 gcgctcgaca tgcccttcat gaagagatat tcgaagtcct atcttgcaaa gcacccggag
     1321 aaaaggggcg acgacctcaa ggcaacccgc caagcctgca ggaagttcag cgccacaccg
     1381 acatcggtaa ttaacttcgt cgaaggcacg cgcttcagcc tggagaaaaa ggaaaggcgc
     1441 gggtcaacgt acaataacct gctgccgcca cgagccgggg gcatcgcgct cgcgttgtcc
     1501 tcgatgggga gcatgttcga tgccatactc gatgtaaccg tggtttatcc gggggaagta
     1561 atcaccttct gggatttgtg ctgcggcgat caacgtcccg tgattgtcga tgtcgtctcg
     1621 cgtccggtgg aagaaaggct gttgcaggga gactatgcga acgacaggga ataccgatcg
     1681 accatccatc gctggctgac cggtatctgg agcgagaaag acgctcgcat cgatgcgttg
     1741 cgggcggatg cggggcagcg ggagccgcga gcggccgacg ccgatctcgc gtagactgcg
     1801 ttggttccct gttcattcga ggcatgcgtc gtgtccaata atagaaactg gagcgtctac
     1861 atgctgcgct gcggcgatgg ttctctctac acgggcatcg ccaccgatgt gaagcgccgc
     1921 ctcgaagagc atcgcgcgga agagggtgca aaatacctgc gtggccgcgg tcctctcgag
     1981 gtcgtattcc agtcggaggt gggaagcagg agcctggcga cacgggccga attaaggatc
     2041 aagaggcttt ccaggtcagc caaggaggag tttatcgccg cccgcggcaa gagtgacctg
     2101 ctggcggacc tgcaaccgca ggaaggcgtt tgctgattac ctgccgggcc tcccccctta
     2161 cgggtatatc gcgaccgtct ggccaaacgg cagcgtgctg atgctaaccg ggtgacggcg
     2221 atccgcagca agctcgttca gccactttgc cgtctttggc aggtcgtcca cgatgtccgg
     2281 aaatagccgc acttcgccaa aaggttgcgt gaagtcttcg tagtgcacgg gaaatacccg
     2341 cgtcgctttc gttgagacga cggtctcgcg ccaatattcg ccaacatacc tgggaccgag
     2401 cgacgcgagc cctgccaccc ccagcatgac cacatcggca gtttctcctg ccagtgcccc
     2461 ttcgacgaag cctccgctgc cctgcacgag cgccgtgccc ttcgggtggc ttatcaatac
     2521 ggaccaggta cctccctcgc gccagtgccg gattggtgcc ggctgcgtca gcggtttgtc
     2581 aatggttccg gaaaacgggg ggccgtttcc aaagccgata ggtgcgtgtc gcgattcgat
     2641 gagcttgatc gtgaaccggc cgaattgccg cgtctcgccg tcggccagga tctggtactg
     2701 gctcacaggc acatcggcgc cgcgcgcaat gttcgccgtc gattccgacc ccagcaccac
     2761 ggcctgcgta cggttcgcga cgaaccctgc atccattgcg tgatcaaagt gggaatgcac
     2821 cgggatgatg gcggcaagcc ggttaatgcg gaactccgcc agcgccgcat tgatcgtggc
     2881 tgcgtcggaa tggacgcggc gctgcgacag caggtcgaac aggccggggc gcgagaacgt
     2941 cccatcgacg agcaactgcg tttcgccatc gtcaaagagc agcgtcgtga tgccgagcca
     3001 tgtcacggta acacgctcgg cggattcgct ggtggagctt gcgacaggcc agtcgaactg
     3061 cgcgacttcc gggcgttcct gccaacgcca ggcgacatac caggcgactg cgatcccgag
     3121 gag
//
LOCUS       DASUOM010000140         7643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_755978,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000140 DASUOM010000000
VERSION     DASUOM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755978"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFY27_02790"
     CDS             complement(<1..728)
                     /locus_tag="VFY27_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5764465.1"
                     /translation="MSTADGAGWQRTSPFAAVFYIGKIYQAIAKNAVQSLAPLAAFLV
                     AFKGDPTQKVIIGVGVFAIGTIVLAFLRYWFFHYRITGNSVLIRAGVFKKTQLDIKFE
                     RIQAVNTQQNVVFRLLGLLTVSFDTAGASGQEGYLPAVREDIAKELRDRIRRMPRARV
                     DEQNVTEEGAPHTQAKTLLQLTSGDMVRIGLSSNRVFLLLVLLGPLSEMMEQRFAEIV
                     EESAVIEYFSSVQTGLLSGLAFGLV"
     gene            complement(715..1236)
                     /locus_tag="VFY27_02795"
     CDS             complement(715..1236)
                     /locus_tag="VFY27_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5764466.1"
                     /translation="MFENAEIMPDQLPAADELEWRNLDPRYARRLQLEALLTAAIVVV
                     AVLTAQLMPLPVDRRLFLVAWVVVVILALSGLLWPLVSVPRKGYAIRDKDIVYRSGVI
                     WRTVTAVPFNRVQHVETSSTPLDRRFTQAKLQLFTAGGSGGDLKIEGMPADVAERLRL
                     FILEKAGASLEHS"
     gene            1344..1420
                     /locus_tag="VFY27_02800"
     tRNA            1344..1420
                     /locus_tag="VFY27_02800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1378..1380,aa:Met,seq:cat)
     gene            1445..2200
                     /locus_tag="VFY27_02805"
     CDS             1445..2200
                     /locus_tag="VFY27_02805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5764467.1"
                     /translation="MLTLTNQTILITGASRGIGAATAKVALEAGATVVGSYNKNPGAL
                     EQLAADHGSDRVHALQADLARPGAGRILWQESLSEVGKIDGLVNNAGIAPATKLDGDY
                     SGWQRDWAQVFAVNTQAVADLCREAILYFKPRGGGRIVTVASRAAFRGDSADYMHYAA
                     SKGALVALMRSIARGYAADRILAFLIAPGWVATEMASIIYEPGNERALAEIPMGEAAP
                     PEEVGNMIVFLLSGMVDHSTGATFDINGASYVR"
     gene            2500..4095
                     /locus_tag="VFY27_02810"
     CDS             2500..4095
                     /locus_tag="VFY27_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764468.1"
                     /translation="MLWLALLTLLLPSIALAQAGADHIPRRDEGEGPYQRLILRGVNL
                     IDGTGAPTRGPVDLVIENDRIAAIHSVGAPLLAIDPEDRPPLDGGGEIDLHGAYVMPG
                     FVDTHLHLHHMESGQGVPSDYILKLWLAHGVTSGRTVGYDGTLAEEMSIKRLLDSNGI
                     TGPRIHLYPFFNTEEAGPIDTPEQARERIRRIKKAGAVGVKFFGAPEEILWAALDEAD
                     KQGLSSTMHHAQLNVLHANVLATSAHGLKGMEHWYGLPEAMFEDRRIQDYPAEYNYNN
                     EQHRFAEAGRLWKQAAEPGSEKWNEVLQTLLERGFSLSPTFTSYLASRDLMRLSRAPW
                     HEMYTLPDLWQYYRPNRDAHGSYWFDWTLEEELDWKENFRLWMKFVNDFKNRGGRVTV
                     GSDAGYIYNLYGFGYVLELELLREAGFSPLEVIHAATIQGARQIGIGNDVGTVQVGKK
                     ADLVVIRENPLANLHVLLGTGTIKLNDETGKVERIGGIDYTIKDGIVYDAKALLDEVA
                     EMVQAEKDRRGIAPGIMPITNAD"
     gene            4181..4537
                     /locus_tag="VFY27_02815"
     CDS             4181..4537
                     /locus_tag="VFY27_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764469.1"
                     /translation="MQLIKWDEVPLETVNESMQRKIITGERMTVARIWFKDGFLVPQH
                     SHEMEQITQVVSGKMRFWFGADRKEKVDLGPGEVVVIPSNLLHEALCIGDVEEVDMWS
                     PRRDDWLNGTDDYLRR"
     gene            4671..7082
                     /locus_tag="VFY27_02820"
     CDS             4671..7082
                     /locus_tag="VFY27_02820"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5764470.1"
                     /translation="MQKPTTEEFKQALLDRLTYSVGKNPGHAVPRDWCVALTLALRDR
                     IVDSWMKTTGRTYETGAKRVYYLSMEFLIGRLLTDAMSNLGLTSVCREALDELGVDFD
                     TTITSEPDAALGNGGLGRLAACFLDSMSTLGIPAIGYGIRYQHGLFRQRLADGWQVEQ
                     AENWLMLGHPWEFERPESNYMVNFGGVADPDKPPEARWEPDETVIASANDTPITGWTG
                     AHVNTLRLWSAKPAGLFDLSRFNQGDYLRAAAQEVLAETITRVLYPDDSTEQGRQLRL
                     SQEYFFTSASMQDLLRRFLRDHNDLRTLPDFVAIQLNDTHPAIAVPELMRLLTDGHNL
                     PPKEALRITRACLSYTNHTLLPEALERWPLKLFQRILPRHLQLIDFIDRQFMASPEVA
                     SANVNVHDLTTVDYSNGGSVRMGNLAFIGSHRVNGVSALHTELMKQTVFKGLHSVYPE
                     RIVNLTNGVTPRRWLYECNPNLRELLNETIGESWVGDLSKIEALKAFADDSEFRRRFK
                     EAKRNNKVRLANEIQKRSGPVVNPEAVFDVHIKRIHEYKRQLLNALHTVAHYQAIKTN
                     PGADWLPVVKLFGGKAAPSYTMAKLIIKLINDIATVVNHDSEVGERLRIVYMSDYNVS
                     RAEVIIPGADLSEQISTAGMEASGTGNMKLALNGALTIGTLDGANIEIRDHVGHDNIF
                     IFGLTAEEVAEKRREGYSPRDIVAANPRLKTVLEAIRNGAFSPDQPDRFAPLVDNLYD
                     SDYFMNTVDFESYCAAHKAAAAAYRDETRWWRSAVLNTAGVGWFSSDRTISEYDEKVW
                     HTRGK"
     gene            7315..>7643
                     /locus_tag="VFY27_02825"
     CDS             7315..>7643
                     /locus_tag="VFY27_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764471.1"
                     /translation="MFRALVSAAVLVLSGFSVSSSAQHDSSVFVLPDTLVWGPASAKL
                     PAGAQIAVIFGDMSKPGELYAFRAKLPDGYRVPPHTHPMDEHVTVIQGVLTLGVGEST
                     DETQMRV"
BASE COUNT         1591 a         2265 c         2396 g         1391 t
ORIGIN      
        1 acaaggccaa atgccagtcc ggacagtagc cccgtctgca cactgctgaa gtattcgatg
       61 accgcgcttt cctcaacgat ctcggcgaat ctctgctcca tcatctcgct tagcggaccg
      121 agcagcacga gtaacaggaa cacgcggttg ctcgagaggc cgatgcggac catgtcgccc
      181 gatgtgagtt gcaggagagt tttcgcctgg gtatgaggcg cgccttcttc cgtgacgttt
      241 tgctcgtcaa cgcgcgctcg cggcatgcgg cggatcctgt cgcgcagttc cttggcgata
      301 tcttcacgca ctgccggcag atacccttcc tggccggacg cgccggccgt atcgaagctc
      361 acggtcagca gtcccagcag gcgaaacacg acgttttgct gcgtgttgac cgcctgtata
      421 cgctcgaact tgatgtccag ctgcgtcttt ttgaagacac cggcgcggat cagaacggag
      481 ttcccggtga tgcgatagtg aaagaaccag tagcgcagga acgcgagaac gatggtgcct
      541 atagcgaata ctccgacacc gatgatcacc ttctgcgtcg ggtcgccctt gaacgcaacc
      601 aggaatgcgg ccagcggcgc gagcgactgc acggcgttct tcgcgatcgc ctggtagatt
      661 ttgccgatat agaaaactgc ggcgaacggc gacgtacgtt gccagccggc gccgtcagct
      721 gtgctcaaga ctcgccccgg ccttctcgag aatgaacagg cgcaggcgct cggccacgtc
      781 ggcaggcatt ccttcgatct tcaggtcgcc gccggagccg ccggcggtga agagttgcag
      841 cttggcctgg gtgaagcgcc ggtcgagcgg cgtgctgctg gtctcgacat gttgcacgcg
      901 gttgaacggc acggcagtca cggtccgcca gatcacgccg gagcggtaca cgatgtcttt
      961 atcccgaatg gcgtagccct tgcgcggcac gctgaccagc ggccagagca ggccggaaag
     1021 cgcaaggatt accaccacca cccaggccac aaggaacagg cgccggtcga ccggcaaggg
     1081 catcaactgt gccgtgagaa cggcgacgac cacgatcgcc gcggtcagaa gcgcctcgag
     1141 ctgcagcctc ctggcgtagc gggggtccag gttccgccat tccagctcgt ccgccgccgg
     1201 cagctgatcc ggcataattt cggcattttc gaacattggc gcccctggca gcccagcggg
     1261 cattataaag gtggcgacca acttgacagc aagcaggacc catcggacaa tttgcgccct
     1321 ttccgccccc tgttccccga aatggtgatg tagctcagcc ggttagagcg acggattcat
     1381 aatccgtagg tcggtggttc aagtccaccc atcaccacca ctcttcgttt ttttggggca
     1441 catcatgctg acacttacca accagactat cctgatcacc ggcgcttccc gcggcatcgg
     1501 agccgcgacg gcgaaggtgg cgctcgaagc tggcgcgacg gtagtgggca gctataacaa
     1561 gaaccccggc gcgctggagc aactggcggc tgatcacggc agcgatcgcg tgcatgcgct
     1621 gcaggcggat ctcgcgcggc ccggtgcggg caggattttg tggcaggaaa gcctgagtga
     1681 ggtggggaag atcgacggcc tcgtcaacaa cgccggtatt gcgccagcga ccaaactgga
     1741 cggcgattac tccggctggc aacgcgactg ggcgcaggtg tttgcggtca acacgcaagc
     1801 cgtcgccgat ctgtgccgtg aggcaattct gtacttcaag ccgcgcggcg gcgggcgcat
     1861 cgtcactgtt gccagccgag ctgcatttcg cggcgacagc gccgactata tgcactacgc
     1921 tgcgagcaag ggtgcgctgg ttgcgttaat gcgatccatc gcgcgcggct acgccgccga
     1981 tcgcattctt gcgttcctga tcgcgcccgg ctgggtcgcg accgagatgg cctccatcat
     2041 ctacgagcca ggcaacgaaa gggcgctcgc ggagatacct atgggagaag cagcgcctcc
     2101 cgaagaagtc ggcaacatga tcgtgttcct gttgtccgga atggtcgacc acagcaccgg
     2161 cgcgacgttc gacatcaacg gcgcaagcta cgttcgctag tgtcaagccc atgtaggagc
     2221 ccgccatgcg ggcgagtgcg atcgaggtta gagaactcgc ccgcattcgc ccgcaaggca
     2281 gccggattta gatatcgttg tcggtgccct tcggcgtggg ctcctacagc gctccttccg
     2341 acttgtgcag agatgccccg gcaaatgttc ccggacctcg aggtcgcctg tggtaaattg
     2401 gccgcgcgct gcaaccgcaa aaacaacgaa ccgattgcca aaacaggggc gggcatgaac
     2461 aggaatttac cagcctcaca ttcacccaaa agtttcagca tgctgtggtt ggccctgctg
     2521 acgctgctgt tgccgtcaat tgccttggcg caggccggcg ccgaccatat tccccgccgt
     2581 gacgaaggcg aaggtccgta ccagcgcctg atactccgcg gcgtcaacct gatcgacggc
     2641 accggcgcac cgacgcgcgg ccccgtggat ctcgtcatcg agaacgatcg cattgctgca
     2701 atccacagtg tcggcgcgcc gttgctcgcc atcgatcccg aagacaggcc gccgctcgat
     2761 ggaggaggag agatcgacct gcacggtgct tacgtcatgc cgggattcgt cgatacgcac
     2821 ctgcaccttc atcacatgga gtccgggcag ggcgtgccat ccgactacat cctgaaactg
     2881 tggctggcgc atggcgtcac gtcaggccgt acagtcggct acgacggcac gctcgccgaa
     2941 gaaatgagca tcaagcgcct gctggattcg aacgggatta ccgggccgag gatccacctc
     3001 tatccgtttt tcaacacgga ggaagcggga cccatcgaca cgccggagca ggcgcgcgag
     3061 cgcattcgtc ggatcaagaa agcaggggcc gtcggagtga agtttttcgg cgcgcctgaa
     3121 gaaatcctgt gggcggcgct cgacgaagcc gacaagcagg gcttgagttc aacgatgcac
     3181 catgcgcaac tgaacgtgct gcacgcgaac gtgctggcga cttccgcgca cggtctgaag
     3241 gggatggagc actggtacgg cctgccggag gcgatgttcg aggaccggcg tatccaggat
     3301 tacccggccg agtacaacta caacaacgag cagcaccggt tcgcggaagc cggaaggttg
     3361 tggaagcagg cggcggagcc cggttcggag aagtggaacg aggtgctgca gacgttgctc
     3421 gagcgtgggt tttccttgtc gccgacgttt acatcctacc tcgcgagccg cgatttaatg
     3481 cgcctgagcc gcgcgccttg gcacgagatg tacacgcttc cggacctctg gcaatactac
     3541 cgcccaaacc gcgacgcgca cgggtcctac tggttcgact ggaccctgga agaggaactg
     3601 gactggaagg agaacttccg cctgtggatg aagttcgtca acgacttcaa gaacaggggc
     3661 gggcgcgtca ccgtcggctc cgacgccggc tacatctata acctgtacgg cttcggttat
     3721 gtcctggagc tcgagctgct tcgcgaagcc ggtttctctc cgctcgaagt gatacacgcg
     3781 gcgacgatcc agggcgcgcg gcagatcggc atcgggaacg acgtcggcac ggtgcaggtc
     3841 gggaagaaag cagacctcgt cgtgatccgc gaaaacccgc tcgcaaacct gcacgttctg
     3901 ctgggcacgg gcacgatcaa gctcaacgac gaaaccggta aggtagaaag gatcggcggc
     3961 atcgactaca cgatcaagga cggcatcgtc tacgacgcga aggcgctgct cgatgaggta
     4021 gcggaaatgg tgcaggcgga gaaggacagg cgaggcatag ctccgggaat catgccgatc
     4081 accaacgccg attgataagc aggaactcgc cacgcggacg agtgcgaggt cgaccaaaca
     4141 gacccggatt ccgtccgcat gacccaatag aggacaaccc atgcagctga tcaagtggga
     4201 cgaggtgccg ctggaaacgg tgaacgagag catgcagcgc aagataatca ccggggagcg
     4261 catgacggtg gcgcgcatct ggttcaagga cggcttcctg gtgccgcaac acagccacga
     4321 gatggagcag atcacgcaag tggtgtccgg gaagatgcgc ttctggttcg gcgccgatcg
     4381 caaggagaag gtggatttgg gacccggaga ggtggttgtg attccctcga acctgctcca
     4441 cgaggcactc tgcatcggcg acgtcgagga ggtcgacatg tggtctccgc gacgggacga
     4501 ctggctcaac ggcaccgacg actacctgcg aaggtagcgg taacgagcct gaacatgatg
     4561 tagaaagacc aaatatggac gcaatggccc atccgtccac atgaagtcgc cgccgttgcg
     4621 catccttccc gtccgttgtt taatgcgccg ctcgcttccc tttagaaccc atgcaaaaac
     4681 caacaacaga agaattcaag caggctctgc tcgaccgcct cacgtactcc gttggcaaga
     4741 accccggcca cgctgtcccg cgcgactggt gcgtggcgct gacgctcgct ctgcgcgatc
     4801 gcatcgtcga ctcctggatg aagacgaccg gcaggacgta cgaaacggga gcgaagcgcg
     4861 tctactacct gtcgatggag ttcttgatcg gccgcctgct gacggatgcg atgtcgaacc
     4921 tcggcctgac ctcggtctgt cgcgaagcgc tcgatgaact cggtgtcgat ttcgatacga
     4981 cgatcacgag cgaaccggac gcggcgctcg gtaacggcgg gttgggacga ctcgctgcct
     5041 gcttcctcga ttcgatgtca acactcggca ttccggcaat cgggtacggt attcgttacc
     5101 agcacggcct gttccgtcag cgcctggccg acggatggca ggtcgagcag gcggagaact
     5161 ggctcatgct gggccatcca tgggagttcg agcgacccga atccaattac atggtgaact
     5221 ttggcggtgt tgccgacccg gacaagccac cggaggcccg gtgggagccg gatgaaacgg
     5281 tgattgccag cgcaaacgac acgccgatca ccggctggac cggagcgcat gtgaatacct
     5341 tgcgcctttg gtcggcgaag ccggcggggc tctttgacct gtcgaggttc aaccagggcg
     5401 attatctgcg cgctgctgcg caggaagtgc tggcagagac aataactcga gtgctgtacc
     5461 cggacgattc gacggagcag ggccgccagc tgcgcctaag ccaggagtat tttttcacgt
     5521 cggcatcgat gcaggatctc ctgcggcggt tcctgcgcga tcacaatgac ctgcgcacgc
     5581 tgccggattt tgtcgcgata cagcttaacg acacgcaccc agcaatcgcg gtgccggaac
     5641 tgatgcgcct gctgacggac gggcacaacc tgccgccgaa ggaagcgctg cgcatcacgc
     5701 gtgcgtgcct gagctacacc aaccacacgc tgcttcccga ggcgctggaa cgctggccgc
     5761 tgaagctctt ccagagaatc ctgccgcgac acctgcagtt gatcgacttt atcgaccggc
     5821 agttcatggc gtcgccggaa gtggcctcgg cgaacgtcaa cgtgcacgac ctgacgacgg
     5881 tcgactacag caacggtggt tcggtccgca tgggcaacct ggccttcatc ggcagccaca
     5941 gggtgaacgg tgtctccgcg ttgcacacgg agttgatgaa gcagacagtg ttcaagggcc
     6001 tgcactcggt ctacccggaa cggatcgtca acctgaccaa cggcgtgacg ccgcgccgct
     6061 ggctgtacga atgcaacccg aacctgcgcg agttgctgaa cgagaccatc ggagagagtt
     6121 gggtcggcga tctcagcaag atcgaggcgc tgaaggcgtt tgcggatgac agcgagtttc
     6181 ggaggcgatt caaagaggcg aaaaggaaca acaaggtacg gctggcgaac gagatacaga
     6241 agcgttcggg accggtcgta aaccctgaag ctgtgttcga tgtgcacatc aagcgcattc
     6301 acgagtacaa gcgccagctc ctgaatgcgc tgcataccgt cgcccattac caggcgatca
     6361 agacgaatcc gggcgccgac tggctgccgg tggtcaagtt gttcggcggc aaggcggcgc
     6421 cgagctacac gatggcgaag ctcatcatca agctgatcaa cgacattgcg acggtggtga
     6481 accacgacag cgaggtgggc gagcgtctca ggatcgtcta catgtcggac tacaacgttt
     6541 cgcgtgccga agtgatcatc cccggagcgg acctttcgga acagatatcc actgcgggaa
     6601 tggaagcatc gggcaccggc aacatgaagc tcgcgctgaa cggcgcgctg acgataggta
     6661 cgctggacgg tgcgaatatc gagattcgcg accatgtcgg gcacgacaac attttcatct
     6721 tcgggttgac ggcggaggag gtcgcggaga agcgtcgcga aggctacagt ccgcgcgaca
     6781 tcgtggccgc gaatcctcgc ctgaagacgg tactggaagc aatccgtaac ggcgcgttct
     6841 ccccggacca gccggatcgt ttcgcgccgc tcgtggacaa cctctacgac agcgactact
     6901 tcatgaacac cgtggacttc gaatcgtact gcgccgctca caaggccgcg gcagctgctt
     6961 accgcgacga gacgcggtgg tggcgttcgg ccgtattgaa tacggcggga gtgggctggt
     7021 tttcctccga tcgtacgata agcgagtacg acgagaaggt gtggcacacg cgcggcaagt
     7081 agcgctcgat cagtcgcaga ccgctgccgt acatcaggct ggccgcaagc cagtgctggt
     7141 cacgcggtct gatagcttac gttcgggttg tcctgatcag gactagactt aacaataagt
     7201 ggcgccattt cctacttgtt atactgcggg cgcattattt ctacttactt aaagtcgcgc
     7261 aagggggcaa gcgcgctggt aatggtcctc acgaagctca gagggaggtg cgagatgttc
     7321 cgagctttgg tgtccgcggc ggtactagtc ctgagcggat tcagcgtatc gagctcagca
     7381 cagcacgatt ctagcgtctt cgttttgcca gatacgcttg tttggggtcc ggcgtcagcg
     7441 aagttgccag cgggtgcaca gattgctgtc atattcggcg atatgtcgaa gcccggcgag
     7501 ctgtatgcct tccgagcgaa gcttcccgat ggataccgcg taccgccgca cacccatccg
     7561 atggatgagc atgtgactgt gatccagggc gtcctgacat tgggcgttgg cgaatctacg
     7621 gatgaaactc agatgcgcgt gtt
//
LOCUS       DASUOM010000141         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1646424,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000141 DASUOM010000000
VERSION     DASUOM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646424"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFY27_02830"
     CDS             <1..826
                     /locus_tag="VFY27_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5764472.1"
                     /translation="ASGEVTGAYALTEPQSGSDARAMRSRAQLSKDGSHYTINAKKSW
                     ITSGPVARYVVLFAQTFSAGGEPAGISAFLIDADADGFHRGKHEPKLGIRASATCEIE
                     LTDYRCPAENRIGEEGAGFRIAMTVLDAGRVGIAAQSLGIARAAYDAALAWSREREAF
                     GTAIGSFQAIQAKIADMRTRIEASRLLVLQAAWAKGEAERTGGKNTLNASMAKLFASE
                     TCMWVTHQALQIHGGMGYSRELPLERYFRDAKITEIYEGTSEIQRMVIGRLETGLR"
     gene            complement(863..1183)
                     /locus_tag="VFY27_02835"
     CDS             complement(863..1183)
                     /locus_tag="VFY27_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5764473.1"
                     /translation="MPTVIYITPDGTRHETEVDTGYSVMEGAINNNIEGIVAECGGAC
                     ACATCHSYIDEAWLGRIPPMDDMEDSMLDAAFERRPNSRLTCQIEVTEALDGLVVYVA
                     DNEY"
     gene            complement(1302..2666)
                     /locus_tag="VFY27_02840"
     CDS             complement(1302..2666)
                     /locus_tag="VFY27_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764474.1"
                     /translation="MLTDIRLRKLAPLLAALLLCGGAAAQEEDAAQRESEAQDTKQAQ
                     AVSKAVYDEITKAQEMVEAKNYQGALQLLQRLYNPDELTEYEQANVLNYIGFVYYNMD
                     NPAKAIETYDKMLAIPSLEAQMRKQVTFTLAQLNMMEERYQRALELLDTWFTLETNPG
                     PEAFILKAQALYQLERYQDMVEPIETGIRIARERDLEIKEDWYVLLNFAYFQQEDYAK
                     VRDIQKILLANWPKKRYWMSLAGAYTELGEDANLISAYSAAHTDGLLESEAEFVTMAQ
                     LFMQSEVPYKAATLLEEKMEDGTVKADEKNYRLLSQAWTLAQEDDKAIPALQEAAERT
                     PDGELDIRLANSYLNIGEYEDCAEAARNGLRKGDLKDPDNAQISLGMCLYNLRQYGRA
                     KEAFQAAARTEKSRRTANQWIQVIDAEVERNRQIQMAQEAARKKQAEVEERRQRSGRL
                     VTGI"
BASE COUNT          440 a          868 c          871 g          589 t
ORIGIN      
        1 agcctccggc gaagtaaccg gagcgtacgc actgacggag ccgcagtccg gttccgacgc
       61 gcgggcgatg cgctcgcgtg cccagctctc gaaggacggg agtcattaca cgatcaatgc
      121 caagaagtcc tggataacct ccggtcccgt ggcccggtac gtggtcctct tcgcccagac
      181 gttcagcgcc ggcggcgagc cggcgggtat cagcgcgttt ctcatcgacg cggacgccga
      241 tggcttccac cgcggcaagc acgaaccgaa actcggcatc cgcgcctccg cgacctgtga
      301 aatcgagctc acggactatc gttgcccggc cgaaaaccgt atcggcgagg aaggggccgg
      361 gttccggatt gcaatgaccg ttctcgatgc cggccgcgtc ggaattgccg cccagtccct
      421 gggaatcgca cgtgccgcat acgacgcggc gcttgcctgg tcgcgcgagc gtgaagcctt
      481 cggcactgcc atcggcagct tccaggcgat ccaggcgaaa attgccgaca tgcgcacgcg
      541 catcgaagcg agccggttgc tcgtgctgca ggccgcctgg gcgaaaggcg aggctgaacg
      601 cacgggtggc aagaacacgc tgaacgcgtc gatggcgaag ctgttcgcgt cggaaacgtg
      661 catgtgggtc acgcaccagg cgctgcagat ccatggcggc atgggataca gcagggagct
      721 acccctcgag cgctacttcc gcgatgccaa gatcacggag atctacgagg gcacgtcgga
      781 gatccagcgc atggtgatcg gccgcctcga aaccggattg cgctagggag cccctgcaca
      841 cgcgcggcgg caacgccgcg aatcagtatt cgttgtcggc aacgtagacg acgaggccgt
      901 caagcgcttc ggtcacttcg atctggcagg tcagccggct gttcgggcgg cgctcgaaag
      961 ctgcatccag catcgagtcc tccatgtcgt ccatgggcgg gatgcggccg agccaggctt
     1021 cgtcaatgta gctgtggcag gtcgcgcaag cgcaggcgcc accgcattcc gcgacgattc
     1081 cctcgatgtt gttgttgatc gcaccttcca ttacggaata gcccgtgtcc acctcggttt
     1141 cgtggcgtgt tccgtcgggg gtgatgtaaa tgactgtcgg catgcctgtg gctgacctcg
     1201 aattcgcgca gcaaatcgta cgaaaggcgc tggccgtttc cggccaacgc cctcgaagtt
     1261 cgcgatgata tcggtttcag gctgaattgt gcagtgtctg cctagatccc ggtgacgagc
     1321 cggcccgagc gctggcgacg ttcctcgact tccgcctgct tcttgcgggc cgcttcctgg
     1381 gccatctgga tctggcgatt ccgctctact tccgcgtcga tcacctggat ccactggttc
     1441 gccgttcgcc gggacttttc ggtccgcgcc gccgcctgga atgcctcctt cgcccgaccg
     1501 tactgccgca ggttgtacag gcacatgccg agcgagatct gcgcgttgtc cgggtccttg
     1561 agatcgccct tgcgcaggcc attgcgggcc gcttcggcac aatcctcgta ctcgccgata
     1621 ttgaggtagg aattggccag ccggatgtcg agttcgccgt ccggcgtgcg ctctgctgct
     1681 tcctgcagcg ccgggatggc cttgtcgtcc tcctgggcga gcgtccaggc ttgcgagagc
     1741 agccggtaat ttttctcgtc ggccttgacc gtgccgtctt ccattttctc ctcgagcagt
     1801 gtcgcggcct tgtaagggac ctcgctctgc atgaagagct gcgccatcgt cacgaactcg
     1861 gcttccgatt cgagcaagcc gtcggtgtgg gccgcgctgt aggccgagat gaggttcgca
     1921 tcttcgccga gctcggtata ggcgccggcc agcgacatcc agtagcgctt cttcggccag
     1981 ttcgcgagca ggatcttctg gatgtcgcgt accttggcgt aatcctcctg ctggaaatag
     2041 gcgaagttca ggaggacgta ccagtcttcc ttgatttcca gatcccgctc ccgggcaatg
     2101 cggatgcccg tctcgatggg ctccaccatg tcctggtagc gttcgagctg gtagagcgcc
     2161 tgggccttga ggatgaaagc ttccggaccc gggttcgtct cgagcgtgaa ccaggtgtcc
     2221 aggagttcga gcgcccgctg gtaccgctct tccatcatgt tgagctgcgc gagggtgaac
     2281 gtcacctgct tgcgcatctg cgcctcgagg ctcgggattg cgagcatctt gtcgtatgtc
     2341 tcgatggcct tcgccgggtt gtccatgttg tagtagacga agccgatgta gttcaggacg
     2401 ttggcctgct cgtactcggt cagttcgtcg gggttgtaca ggcgttgcag gagctggagt
     2461 gcgccctggt aattcttcgc ctcgaccatt tcctgtgctt tcgttatctc gtcgtagacc
     2521 gccttgctga ccgcctgcgc ctgcttcgtg tcctgggctt cggattcccg ctgcgccgcg
     2581 tcttcttcct gcgctgcggc gccgccgcac agcaggagcg ccgcgagcag tggcgcgagt
     2641 ttccgcaatc taatgtcagt aagcatcgtt cctccaggtc tgtcgatccc gcctgcgttt
     2701 cgccgtcatt cctcgatctt gaatgaaatg cgggtcttga cgttgggcac ggcgaccggc
     2761 actccgtc
//
LOCUS       DASUOM010000142         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1490966,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000142 DASUOM010000000
VERSION     DASUOM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490966"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFY27_02845"
     CDS             complement(<1..541)
                     /locus_tag="VFY27_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5764475.1"
                     /translation="MLPVVALVGRPNVGKSTLFNRLTRSRDALVADFPGLTRDRQYGF
                     GKQGPVPYLVIDTGGVAGGETGLSELMVQQTVRALKEADIAIVLVDGRAGLTAADERV
                     AELARLHAEKTWLVANKAEGMDPHLAAVEFHRLGLGEPVAISAAHGDRVAALMDEVLA
                     PFVEPDAEPPPDEAEDPSRE"
     gene            complement(601..1797)
                     /gene="bamB"
                     /locus_tag="VFY27_02850"
     CDS             complement(601..1797)
                     /gene="bamB"
                     /locus_tag="VFY27_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEX5764476.1"
                     /translation="MRKFSVHILATCCASVVLLSGCGIFGGDKDEELQPQELVDFKST
                     LPVDRLWEAKVGGGFELLRITLKPAGNAESVYAASRDGNVTAFDPATGERLWRTELKL
                     PLSAGPGFGRSRVVVASSDGFLVCLDADDGREIWRTYIAGESLAPPLVQGDSVVAYTI
                     DGRLSVRSLYNGSERWTLEQALPPLTLRGSAPPVIVGTTVIAGFDNGRLIASGLLDGT
                     TRWEVMLSPPTGRSDLERLSDIDGTLATVGQDVYATAYQGQLAALAAESGQILWTRDI
                     STHVGLDADAENLYVVSETGELIALRRSDGSELWRHDMLLRRKPTAPVTFMDTVVVGD
                     FEGYVHFFSKADGAPVARLRVGKGMLSGPAAVIDGRLYVQSESGRLAAFEIETPDAET
                     TAHNAE"
     gene            complement(1794..2561)
                     /locus_tag="VFY27_02855"
     CDS             complement(1794..2561)
                     /locus_tag="VFY27_02855"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5764477.1"
                     /translation="MDELLSEKEQIEALRSWWRENGRFIISGLVLGVALLLGFNYWNK
                     QKAENALEASAAYEALLSEVAKGDAEAAGERAAALEESYGSTVYAAQARLAMAKLYMD
                     KGRDEDAATELKALIEAGDATDAREAVMIGRLRLAKILLYQDKPQEAIDLLQGYGDTA
                     FAGRYSEALGDAYVALGQAEDAREAYTLALADDPNAPTVNRTLVQMKINDLPKADAPA
                     ADELPSPPPADTPPGDAAADVPAEDPIPEDADAGAAQ"
     gene            complement(2563..3855)
                     /gene="hisS"
                     /locus_tag="VFY27_02860"
     CDS             complement(2563..3855)
                     /gene="hisS"
                     /locus_tag="VFY27_02860"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5764478.1"
                     /translation="MNDVLPGISGTWRHVEEVVRDVVEAFGYSEIRLPLLEYTELFRR
                     SIGEVTDIVEKEMYTFEDRNGESLTLRPEATAGVVRAGITNGLLYNQRQKLWTAGPMF
                     RYEKPQQGRYRQFHQIDVEAMGFEGPDVDAELILMCRRLWGRLNIPRVALQINSLGNP
                     ETRRTYRSELVRYFSAAKDRLDEDSLRRLEQNPLRILDSKNPDMHELVAGAPVMLDYL
                     DDESARHFEELKSLLGAAKLSYTVNPRLVRGLDYYNRTVFEWVTDALGSQGAVCSGGR
                     YDGLVEKLGGRPVPAVGWAMGVERLVALYEICGNEAPGRNPDVYIVAVGAPAVRRGLE
                     LAEKLRDELGTLRFEMNLGDGSFKAQLKRADRSGATYAVILGDEEVRDNRASLKPLRS
                     SGDQVSVPFDSLASVLAGRITPTVARTGDFAESTDKRQ"
     gene            complement(3894..>4597)
                     /locus_tag="VFY27_02865"
     CDS             complement(3894..>4597)
                     /locus_tag="VFY27_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX5764479.1"
                     /translation="VFAKGYLRKYAALVGIPVDDILADYNRMNTSASVPLVIPHRAPP
                     PREISPGPWLGGITLAAVAAAVAWWWMSSGPDWLDDEAETAALEPFADDGEARPANES
                     QPEANQAQANVQPPAEDVEMETPEPVADELSRQDLAQAQPAEESGLPNEGEVELRVTF
                     SGDCWTEVTDAGGDRLYFGLGSAGRSVTVSGEAPLQVLLGNSVNASLEVNGSTYAIPR
                     SARRGDTARLTIANL"
BASE COUNT          784 a         1534 c         1390 g          889 t
ORIGIN      
        1 gctctctaga tggatcttcc gcctcgtccg gtggcggttc ggcatccggt tccacgaagg
       61 gggccaggac ttcgtccatc aacgcggcca cgcggtcgcc gtgtgcggcg gaaatcgcaa
      121 caggttcgcc caggcccaaa cggtggaact cgacggccgc gaggtgcggg tccatgccct
      181 cggccttgtt cgccaccagc cacgtcttct ccgcgtgcag gcgcgcaagc tcggcaaccc
      241 gctcgtcggc tgcagtcagg ccggcccttc cgtcgaccag cacgatggca atatcggcct
      301 ctttcagcgc ccggaccgtc tgctgcacca tgagctcgct taagcccgtc tcgccgccgg
      361 cgacgccgcc ggtatcgatg acgagatatg gaacaggccc ctgcttgccg aacccgtact
      421 gacggtcccg cgtaagcccg ggaaaatcag cgaccagcgc atcgcgcgag cgggtcaggc
      481 ggttgaacag ggtcgactta ccgacattcg gccgtccgac gagtgcaaca actgggagca
      541 ttcgagttcc ttcagtagga cgctccgggt taaggactta agcccggtcg atggtctggc
      601 ctactctgcg ttgtgagcgg tggtctccgc atcgggcgtc tcgatttcga acgcggcaag
      661 ccgccctgac tcgctctgca cgtagagacg tccgtcgatc acggcggccg ggccggacag
      721 catgcctttg ccgacgcgga gcctggccac cggcgcgcca tccgctttgc tgaagaagtg
      781 cacatatcct tcgaagtcac cgaccaccac ggtatccatg aaggtgacgg gcgcggtggg
      841 cttgcggcgc aggagcatat cgtgacgcca caattcggaa ccgtcgctgc gccgcaaggc
      901 gatcagttcg ccggtttcgg acaccacgta gagattctcc gcgtccgcgt ccaggcccac
      961 atgagtcgag atatcgcgag tccacaaaat ctggccggat tctgccgcaa gcgctgcgag
     1021 ctggccctgg taagcagtcg cgtagacatc ctggccgact gtcgcgaggg tcccgtcgat
     1081 gtccgaaaga cgctcgaggt ccgaacggcc tgtcggcggc gacagcatca cttcccagcg
     1141 cgttgtcccg tcgagaaggc ccgacgcaat cagcctgccg ttgtcgaatc cggcaatgac
     1201 cgtagtgccg acgatcaccg gaggtgctga acctctgagc gtcagtggcg gaagcgcctg
     1261 ctccagtgtc cagcgctcgc tgccgttata cagggagcga acgctcaggc gtccgtcgat
     1321 ggtgtaggcg accacggaat caccctgcac cagcggtggc gcgagggact ctcccgcgat
     1381 gtaggtacgc cagatttccc tgccgtcgtc tgcgtcgagg catacaagga agccgtcgct
     1441 gctcgcgacg acgacgcggc tcctcccgaa gcctgggccc gcggacaacg gcagcttcag
     1501 ctccgtgcgc cacaggcgtt caccagtcgc cgggtcaaaa gcggtgacat tgccatcgcg
     1561 gctcgccgcg tatacggatt ccgcgtttcc tgccggcttc agcgtgatgc gcagcagttc
     1621 gaagccacca ccgaccttgg cttcccagag gcggtcgacg ggaagcgtcg acttgaaatc
     1681 cacgagttcc tgcggttgca gttcctcgtc cttgtcaccg ccgaagatgc cgcaccccga
     1741 gaggagaacc acggacgcac agcaggtcgc aaggatgtgg acagagaact tcctcattgc
     1801 gcggccccgg cgtccgcatc ctccggtatg gggtcttccg ccgggacatc cgccgcggcg
     1861 tctccagggg gagtatctgc cggtggtggc gatggaagct cgtcagccgc cggcgcgtcc
     1921 gccttcggca aatcattgat cttcatctgc acgagagtgc ggttgacggt gggggcgttc
     1981 gggtcgtccg cgagcgcaag cgtgtaggct tctcgcgcat cctcggcctg cccaagtgct
     2041 acgtaagcat cgcccagtgc ttcgctgtag cgccccgcga acgcggtatc gccgtagccc
     2101 tgcagcaggt ctatcgcttc ctgcggcttg tcctggtaca ggagtatctt cgcgagccgc
     2161 agacgcccta tcatcacggc ctctctcgca tctgtggcgt ccccggcctc gatcagcgcc
     2221 ttgagttccg tcgctgcatc ttcgtcgcgc cccttgtcca tgtacagctt cgccatggcg
     2281 agcctcgcct gggccgcata gaccgtggac ccgtagctct cctcgagagc ggccgccctt
     2341 tcgccggcag cctctgcatc gcccttcgca acttcgctca gcaatgcctc ataggccgcc
     2401 gacgcttcca gagcattctc tgctttctgc ttgttccagt aattgaagcc aaggagcagc
     2461 gcgacgccca ggacgaggcc gctaatgatg aagcgcccgt tctcgcgcca ccagctgcgc
     2521 agagcctcta tctgttcttt ctccgataaa agttcgtcca cgttactgcc ttttgtctgt
     2581 tgattctgcg aagtcgcccg ttcttgcgac ggttggcgtg atgcgcccgg ccagcaccga
     2641 ggccaggctg tcgaagggta cgctgacttg gtccccgctg ctgcgtaaag gtttcaggga
     2701 ggcgcgatta tcccgcactt cttcatcgcc cagtattacc gcgtaggtgg cgccgctcct
     2761 gtccgcacgc ttcagctgcg ccttgaacga gccgtcaccg aggttcattt cgaacctcag
     2821 ggtaccgagt tcgtcccgca gcttttccgc aagttccagg ccgcgcctta ccgctggcgc
     2881 gcccaccgcc acgatgtaga cgtcagggtt gcgcccggga gcctcgttgc cgcagatttc
     2941 gtacagcgca accagcctct cgacgcccat agcccagccg accgcaggca ccgggcgtcc
     3001 accgagtttt tcgaccaggc catcgtaacg gccgcccgag caaaccgcac cctgcgaacc
     3061 cagcgcatcc gttacccact cgaaaaccgt tcgattgtaa tagtcgagcc cccgcacgag
     3121 ccgcggattg acggtgtagg agagcttcgc ggcaccgagg agactcttga gctcctcgaa
     3181 atgtcgcgcg gattcatcgt cgaggtagtc gagcattacg ggagcgccgg ccaccagttc
     3241 gtgcatgtcg ggattcttgc tgtcgagaat ccgcagcggg ttctgttcca ggcgacgcaa
     3301 actgtcttcg tccagccggt ctttcgctgc cgaaaagtag cgtacgagtt cgctgcggta
     3361 ggtgcgacgc gtctccggat tgccgagcga gtttatctgg agcgcgaccc ggggaatgtt
     3421 cagccggccc cacaggcgcc ggcacatcag tatcaattcc gcgtcaacgt cggggccctc
     3481 gaagcccatt gcttcgacgt cgatctggtg gaactggcgg tagcggccct gttgcggctt
     3541 ctcgtaacgg aacatcggcc cggccgtcca cagcttttgc cgctggttgt acagcagtcc
     3601 gttggtgatg ccggcccgaa ccacgcctgc agttgcttcg ggacgcagcg taaggctttc
     3661 gccattacgg tcttcgaagg tgtacatctc cttctcgaca atgtcggtta cctcgccgat
     3721 cgagcggcgg aacaattcgg tgtactcgag cagcggcagg cggatttccg aatagccgaa
     3781 cgcttccacg acgtcgcgca ccacctcttc gacgtggcgc catgtcccgg agattcccgg
     3841 aagcacgtca ttcatgcccc tgatggcttt aggcttcacg ggatccttcg agatcagaga
     3901 ttggcgatcg tgaggcgagc ggtgtcgcca cggcgcgcac tgcgcggtat ggcgtaagtc
     3961 gagccattca cctcgaggct cgcgttgacg ctgttgccca gcagtacctg cagcggcgcc
     4021 tcccccgaaa cggtaacgct tcggcccgcc gagcccaacc cgaaatagag acgatcgccg
     4081 cccgcatccg tcacctcggt ccagcaatcg ccgctgaagg tgacacgaag ttcgacctcg
     4141 ccctcattgg gcaaacccga ttcttccgcg ggttgtgcct gcgccaagtc ctgccgtgat
     4201 agctcatcgg cgaccggttc gggtgtttcc atttccacgt cttcagctgg cggttgcacg
     4261 tttgcctgcg cctggttcgc ctccggctgc gactcgtttg ccggccgagc ttccccgtcg
     4321 tcggcgaacg gctcgagggc ggcggtttcc gcctcgtcgt cgagccaatc cgggccactc
     4381 gacatccacc accaggcaac agcggcggcg acggcagcaa gcgttattcc gcccagccag
     4441 ggacccggcg aaatctctcg cggcggcggc gccctgtggg gaatgacaag cggcacgctg
     4501 gcggaagtgt tcatgcggtt gtagtccgcg aggatatcgt caaccggtat accgacgagc
     4561 gccgcatact tccgcagata acctttcgcg aagacag
//
LOCUS       DASUOM010000143         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1798554,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000143 DASUOM010000000
VERSION     DASUOM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798554"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..445)
                     /locus_tag="VFY27_02870"
     CDS             complement(95..445)
                     /locus_tag="VFY27_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HEX5764480.1"
                     /translation="MRKLTYTAFVAFWACVVTLVVTEALVSSPEEEHVVPAYTLAQVA
                     EHATSTDCWMAIEGKVYDVSSYVPRHPAPPSVITAWCGKEATEGMRTKGYGPDHSPAG
                     WTLLEQFLLGELAR"
     gene            complement(442..1755)
                     /locus_tag="VFY27_02875"
     CDS             complement(442..1755)
                     /locus_tag="VFY27_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEX5764481.1"
                     /translation="MHPAIARAIANTVLWLLLVLPFFLALLQARGPDFADAGAVLNWL
                     GRLAGIAGLTCLLLAAALIIRVPGFDRLFGGLTSLWKIHHRLGAASFMLLLAHPLFLA
                     FAASNVSLSAAVDVLFPPDERVPALFGWSALVIMMIFLAPTFAFFGQPRYQRWKWVHR
                     SSGAAVVLALVHTLMAGRTFPPPYDWFLWLALALLAATAITYRFLFSRRLGRRRYRVA
                     GVAHPANNVVELRLEAEGSPLRYRAGQFVYLTPYDRNLASGYAEEHPYTLSSSPAEEH
                     LRVAIKDLGDSSRALQHVTPGSPILVEGPYGDFFPDHFGRELWIAGGIGITPFLARAR
                     HLAATGETADVVLIFDVQDEARAMFCAELEALAQATKGFVLVLHFFYLEGPLNREFLE
                     RHCPDFAERDAYLCGPLPLKTLAQQYLLEGGSNIERVYTEEFVLL"
     gene            2162..2368
                     /locus_tag="VFY27_02880"
     CDS             2162..2368
                     /locus_tag="VFY27_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5764482.1"
                     /translation="MSTTTGTVKWFNDSKGFGFIEQESGPDVFAHYNSIVGEGRKTLH
                     EGQKVQFTVTQGQKGPQADNIVAI"
BASE COUNT          519 a          742 c          750 g          389 t
ORIGIN      
        1 gatactacgc gcagatgtgg cggctgcagc aggaagaacg caaggtcgga gtggaaccgg
       61 gcctggcgac ggcgtagtta aggagcgcgt tcgtttaccg ggccagttca ccgagcagga
      121 attgctccag cagcgtccac cctgcggggc tgtgatccgg accgtaaccc ttggtgcgca
      181 ttccttccgt tgcttccttg ccgcaccacg ctgtgatgac agagggaggc gccgggtgcc
      241 gcggcacgta gcttgagacg tcgtagacct tgccctcgat ggccatccag cagtccgtcg
      301 aggtagcgtg ttccgcgacc tgcgccagcg tgtacgccgg caccacatgc tcctcttcgg
      361 gagacgatac cagcgcttcc gtaacgacca gcgtcaccac acatgcccag aaggccacga
      421 acgccgtgta ggtcagcttc ctcacaacag tacgaattcc tcggtgtaga cgcgctcgat
      481 gttgcttcct ccttccagga gatattgctg agcgagggtc ttgagcggta acggcccgca
      541 aaggtacgca tcccgctcgg cgaaatcggg gcaatgccgt tccaggaact cccggttcag
      601 cggaccttcg agatagaaga agtgcagcac cagcacaaaa cctttggttg cctgcgccag
      661 cgcttccagt tccgcgcaaa acatcgcgcg tgcctcgtcc tgcacatcaa agatgagcac
      721 gacgtcggcg gtctcaccgg tggctgcgag gtgccgagcg cgcgcaagaa acggcgtgat
      781 acctatgccg ccggcaatcc acaactcgcg tccgaagtgg tccgggaaga aatcgccgta
      841 ggggccttcc accaggattg gtgaacccgg cgtgacgtgc tgcagggccc ggctggaatc
      901 gcccagatcc ttgatcgcca cccgcaggtg ctcctcagcc ggcgacgagg acagcgtgta
      961 ggggtgctcc tccgcgtacc ccgacgccag gttccggtcg taaggcgtga gatagacgaa
     1021 ctggccggcg cgatagcgga gcggactgcc ctccgcctcc agtctcagct cgacgacgtt
     1081 attggccggg tgagcgacgc cggcgacacg atagcggcga cgccccagcc gtcgggagaa
     1141 caggaaccgg tacgtgatcg ccgttgccgc gagcagagcc agggcgagcc acaggaacca
     1201 gtcatacggc ggcgggaagg tccgccctgc catcagcgtg tgcaccagcg cgagcacgac
     1261 cgccgctccc gaactgcgat gtacccactt ccagcgctgg taacgaggct gcccgaagaa
     1321 ggcgaaggtc ggcgccagga agatcatcat gatgaccagt gccgaccagc cgaaaagcgc
     1381 cggcaccctt tcgtccgggg ggaacaggac atcgaccgcc gcggacagcg acacattcga
     1441 cgcggcaaac gcaaggaaca gcgggtgtgc gagcagcagc atgaaagagg cggcgccgag
     1501 tcggtgatga atcttccaaa gcgacgtcaa gccaccgaag agccgatcga agccggggac
     1561 acggatgatc agcgcagcgg cgagcagcag gcaggtgagc ccggcaattc cggcaagtcg
     1621 tcccagccag ttgagcactg cacccgcgtc cgcaaagtcg gggccgcgcg cctgcagcaa
     1681 cgcaaggaaa aaagggagga ccagcaacag ccagaggacg gtgttcgcta ttgcccttgc
     1741 gatcgccgga tgcatcaacg ctctgcgaag accggcatca ggatgagcgc cgatggcttg
     1801 tgaaggcgga aacatccatg caacgatgtt agcagccaga gttaccccgg gtctgcccgc
     1861 ggggatccgc cgcgttgcgg gagcctgcct gcgggcgagt gtcagtggcc tcagcgaact
     1921 cgcccgcggg gcgggctcct acaggagccg ggctcgaccg gcaatattaa gcttgactat
     1981 tgaagggcac tgtatagact cagcccggct tgagcgtgcg ctttatattt atgccaccgc
     2041 gtcgaagttc ttcatttgtg cctcgtcctg taactcataa gtaacccgca accagctaag
     2101 cgacatctgc ccgagtcctc cgaccgggct tacattactc atgatttgac aggataaaga
     2161 tatgtctaca actacaggca ccgttaaatg gttcaacgat tccaagggct tcggcttcat
     2221 cgagcaggaa tcgggtcccg acgtattcgc ccattacaac tcgatcgtcg gcgagggccg
     2281 caagaccctg cacgaaggcc agaaggtcca gttcaccgtc acgcagggcc agaaaggtcc
     2341 ccaggcggat aacatcgtcg cgatctgaca atcacggtgt gaaaacagga gggcggagcc
//
LOCUS       DASUOM010000144         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1618573,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000144 DASUOM010000000
VERSION     DASUOM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618573"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFY27_02885"
     CDS             complement(<1..182)
                     /locus_tag="VFY27_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764483.1"
                     /translation="MQPPFDIAADLDTPVSAYLKLKELKPRFLLESVEGGERLARYSF
                     LGFGDGFEVRLDRNTF"
     gene            complement(389..2224)
                     /locus_tag="VFY27_02890"
     CDS             complement(389..2224)
                     /locus_tag="VFY27_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5764484.1"
                     /translation="MQPITPDAHGVAVLLLTAVAFVLFTRDRIPLETSSLTILIILIA
                     GFQIFPYPDDTIALDARDFFAGFGNEAMIAIGALLMVGKALDTTGAMQPLATVIGRTW
                     SKWPAVALLITLAVSAVMSAFLNNTPIVILLMPILVGVSLKTKTPLSGLMMPLGFATI
                     IGGMSTTIGASTNLLVAGIARDMGFVDFGMFDFVLPVAIVGSIGILFLWLIAPRLLPE
                     RIPPMADVSPRVFSGQLHIKEGGFAAGKTLSEILSKTDGRMRVDRVQRGESLFLAKLP
                     SLVLQPGDRLFVKDTPENLKQYEAMLGTTLYSSADTEHPVDADRPLTAAGQQLAEVVV
                     TRGSPLHFRNLASTRFSSMYGLSPLAIHRARVPSSQITTDLNQIRLRAGDVLLVQGTR
                     SAIDKLKAGGSMLVLDGTTDLPHTERAKTALAILAFVILVAAIDLLPISVSALIGVGL
                     MLATGCLRWRDALGALSTRVILVIVTSLALAKALVGTGMASLLALGFVSAAGDLPVPV
                     VLSALLLIMILLTNVVPNTAVAIVSTPIALAVAFQLGSDPEPFVLAVLFGASMSFATP
                     FGYQTNLLILSAGGYRFSDFIRVGIPLSIIMWIGYSIVLPLLYDA"
     gene            complement(2343..>3719)
                     /locus_tag="VFY27_02895"
     CDS             complement(2343..>3719)
                     /locus_tag="VFY27_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764485.1"
                     /translation="NPPSVQGRAIMLENTNLEGAPLQREVPRAAGSAGGGVEELAEHL
                     ASHKVYPRLLQSGNGITAGGAAIRVSVQQALQSQLTAEHLMRRVTLLVHEQVPLTLAL
                     WELGAEDESVTALEDFCGILHAALAAEGLAAGQVGISMQSHLVPLKAYLLICTAILGR
                     GPRYIVLDSLQMRHHDCDVVQAEADSNWSFLWSVRNASATVVPAYAASVTTRCPLLGD
                     ETATAVLPEAGQQAPVESAWLPMQIRLPDFSDGRGRLHWESLETALQQAVETGDQILE
                     HLYWHLPQQRNDAWQNRRLAITVDGLGDLVVERGADPADLRCLQWIDGAVARIHAILW
                     DRSRTLAQQTEPLPALLSNWPAVSWDCDIKKSDWDVRWRRAVANSAVRHRNMLVLSPY
                     AVLPRCSAASYDFVDLLPVLHHADAFSFANPRVDGFRNCTEFATFHRRAWAVMQRRNA
                     ASLVAAGV"
BASE COUNT          714 a         1148 c         1203 g          654 t
ORIGIN      
        1 tggaatgtgt tccggtccag ccttacttca aagccatcgc cgaagcccag gaaagaatag
       61 cgcgccagcc gctcgccacc ctcgacactt tccagcagga accggggttt gagctccttc
      121 agtttcaggt atgccgaaac aggggtgtcg aggtcggcgg ctatgtcgaa cggcggttgc
      181 attctttgat caagatctcg tcagaaaccg gttttgtcgt ggcgaaaaaa aacccacctc
      241 cactttcggc ggagatgggc ttcgtgttct ttctgatcgc agctaaacgt caggcagagg
      301 ccatctccgt ttcaggaagc cagagccagt tcaggtcgtt acggtgcgat gccatatcgc
      361 cggagtatcc ctgcacttcg aagcccggtc aagcatcata aagaagcggc aggacgatcg
      421 agtagccgat ccacatgatt atcgagagcg ggatgccgac cctgatgaag tctgaaaaac
      481 gatagccgcc ggcgcttaag atcaggaggt tggtctggta gccgaacggc gtcgcaaagc
      541 tcatgctggc gccgaagagc actgcgagga cgaagggttc ggggtcgctc ccgagctgga
      601 atgcgaccgc gagtgcaatc ggcgtgctga cgatcgcaac cgcggtgttc ggcacgacat
      661 tggtcagcag aatcattatc agcagcaagg cgctcaacac tacgggcacc ggcaggtcgc
      721 ccgcggcgct gacgaaaccc aaagccagca ggcttgccat gcccgtgccg acgagagctt
      781 tcgccagggc caggctcgtc acgatcacca ggatcacccg tgtcgacagc gcacccaggg
      841 cgtcgcgcca gcgcaggcaa cctgttgcca gcatcagccc gacgccgatc agcgcgctga
      901 cggatatcgg cagcaaatcg atggccgcca caaggatcac gaatgccagt atggcgagag
      961 ccgttttcgc acgctcggta tgcggcaggt cggtcgtgcc gtcgagaacc agcatgctgc
     1021 cgcccgcctt gagcttgtcg atcgcgctgc gcgtgccctg gaccagcagc acgtcgcccg
     1081 cgcgcagacg gatctggttc aggtcggtcg tgatctgcga actcggcacg cgcgcccggt
     1141 gaattgccag cggagaaagg ccatacatac tgctgaaacg cgtagaggcg aggttgcgga
     1201 aatgcagtgg agagccacgc gtgacgacga cctcggcaag ctgctggccg gccgcggtaa
     1261 gcggacgatc ggcatcgacc gggtgttcag tgtccgccga gctgtagagc gtcgtaccaa
     1321 gcatcgcctc gtattgtttc aggttctccg gcgtgtcctt gacgaacagg cggtcgcccg
     1381 gctgcaggac gagtgacggc agctttgcga gaaaaagcga ttcgccgcgc tgcacccggt
     1441 cgacccgcat gcgcccatcc gtcttcgaca ggatttcgga gagggtcttg ccggcggcga
     1501 atccaccttc cttgatgtgc aactggccgc tgaacacccg cggcgatacg tccgccatcg
     1561 ggggtatccg ctccggcagc agccgcggcg caatgagcca gaggaacagg ataccgatgc
     1621 tgccgactat cgctaccggc agaacgaagt cgaacatgcc gaagtcgacg aatcccatgt
     1681 cacgggcgat acctgcgacg agcaggttcg tggaagcacc aatggtggtg gacatgccgc
     1741 cgatgatcgt cgcaaaaccc aaaggcatca tcaggcccga caacggcgtt ttcgtcttga
     1801 gcgaaacccc tacgagtatc ggcatcagga gtatgacgat aggcgtgttg ttgaggaaag
     1861 cgctcatcac ggcactcact gccagcgtga tgagcagcgc gaccgccggc cacttcgacc
     1921 aggtacggcc gatcacggtg gcaaggggtt gcatcgcgcc ggtggtgtcg agcgctttgc
     1981 ccaccatgag tagcgcccct attgcgatca tcgcctcgtt gccgaaaccc gcgaagaaat
     2041 cccgcgcgtc gagagcgatc gtatcgtcgg gataagggaa gatctggaag cccgcgatca
     2101 ggatgatcag tatcgtcagg ctcgacgttt cgagcgggat gcgatcgcgg gtaaacagca
     2161 cgaacgccac tgcagtcagc agcaggacgg cgactccgtg tgcgtcgggc gtaatcggtt
     2221 gcaaagcttc ctcgcggacc tggttcttat gttcgccgca gcgcgcggct agcagcctgt
     2281 cggacctgaa ttgaccggtc gccgtgtccg gtggacgttt cggtgagtga ccgtatatca
     2341 ggtcataccc cggccgcaac gagagaggcc gcatttctgc gttgcatgac agcccatgcg
     2401 cggcggtgaa acgtggcgaa ttcggtgcag ttgcgaaagc cgtccacgcg cgggttggcg
     2461 aagctgaaag cgtccgcgtg gtgcagcacc ggtaggaggt cgacgaagtc atagcttgcc
     2521 gccgaacatc gcggcagcac cgcgtaaggc gacagtacca gcatgttgcg atgccggacg
     2581 gcagagttgg cgaccgcgcg ccgccaacgc acgtcccagt cgcttttctt tatgtcgcaa
     2641 tcccagctca cggccggcca gttggaaagc agcgccggca agggctcggt ctgttgggcg
     2701 agggtcctcg aacgatccca caagatcgca tgaatgcggg cgacggcgcc gtcgatccac
     2761 tgcaggcagc ggaggtcggc cgggtccgca ccgcgctcca ccacgaggtc gccaaggccg
     2821 tcgaccgtta ttgccagcct gcgattttgc caggcatcgt tgcgctgttg cggcaggtgc
     2881 cagtagaggt gctcgaggat ctggtcgccc gtctctaccg cctgttgcag cgctgtttcc
     2941 agcgactccc agtgcagccg gccgcgcccg tcgctgaaat cgggcagcct gatctgcatc
     3001 ggcagccagg cggattcgac cggcgcctgc tgccccgctt cgggcagcac ggccgtcgca
     3061 gtctcgtccc cgagcagcgg gcagcgggtg gtcacgctcg cggcgtaggc aggaacgacg
     3121 gttgcagagg cgttgcgcac cgaccagagg aaggaccagt tgctgtctgc ctccgcctgc
     3181 acgacgtcgc agtcgtgatg ccgcatctgc aggctgtcga ggacgatgta gcggggaccg
     3241 cgaccgagga tcgcggtgca gatcaggagg taagccttga ggggcacgag gtgcgactgc
     3301 atcgatatgc ccacctgccc cgcggcaagc ccctcagccg cgagtgccgc gtgcaggatg
     3361 ccgcagaagt cttccagcgc ggttaccgac tcgtcttccg cgccgagctc ccacagtgcc
     3421 agcgtcagcg gcacctgttc gtgcaccagc agggtgacgc ggcgcatgag gtgttcggcc
     3481 gtcagttgcg actgcagcgc ctgctggacc gacacccgga tggcagcacc gccggcggtg
     3541 atgccgttgc cgctctgcag gaggcgtggg tagaccttgt gcgatgccag gtgttcggcg
     3601 agttcctcta ctccgccgcc cgcgcttcct gccgcgcgcg gcacctcgcg ctgcagcggc
     3661 gctccttcga ggttcgtatt ttccagcatg atcgcccttc cctggaccga tggcggatt
//
LOCUS       DASUOM010000145         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_975670,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000145 DASUOM010000000
VERSION     DASUOM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975670"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..683
                     /locus_tag="VFY27_02900"
     CDS             201..683
                     /locus_tag="VFY27_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5764486.1"
                     /translation="MPSFDVVSEFDSHEASNAVDQANREVDTRFDFKGTGSRYVLEAN
                     VITMTSQTDFQLQQMLDILRQKLAKRGIDIACMKEEQPEISGSEARQKIILRQGIDSD
                     LARNLVKKIKASKIKVQAAIQGEKLRVSGKKRDDLQAVIALLKEADAGLPLQYENFRD
                     "
     gene            695..>3422
                     /gene="ppc"
                     /locus_tag="VFY27_02905"
     CDS             695..>3422
                     /gene="ppc"
                     /locus_tag="VFY27_02905"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5764487.1"
                     /translation="MPTALRRQDITFEDKDQPLRDDVRTLGGLVGELLKEQGGEKLFD
                     FVENARVQSIRRREGNEKPGEKLEDLFDNLDLGFALEVIRSFSTYFQMVNTAEKVHRI
                     RRRREYLQEEGKYQPGGLEETLVKLKAGGVDVPRLQALLDSIRIEPVFTAHPTEPTRR
                     TILRKEQNIVRQLVNMLNPTMTAQEKNVAIQNIKMEATTGWQTNEHPSEQMTVADELE
                     HVLFFVTDVLYRTIPPFYEDLESAAVRIYGEEARDLHVPGLLRFASWVGGDMDGNPNV
                     NAKTIRETLARQRSLILDLYYRECGKLSAKLSQSKTRVGVEDAVYAKIEEYTPAFQHA
                     YHAVPARHRDMPYRVFLRLVQQRLQATYDDDAYPYESPEEFYADIDLIARSLAANKGE
                     HAGLFAVRRLLRRIATFGFHLVTLDVRQDALVHREVVGECIGDDKWLERSPEYRVEKI
                     AEAILQKDPPVLEQSTKARKTLAVFQAIAFCRRKYGAAAIGPFIVSMTQGVDDILSVI
                     LLSEWSELHNRRGEVPLDIAPLLETVDDLKRGPQILRTLLSHEIYRQHLEQRGKRQTV
                     MIGYSDSNKESGIAAARWALQEAQIDLVQAVAAEDVVLQLFHGRGGTISRGGGRTHAA
                     VLGAPPGTVNGYLRVTEQGEIINEKYGLRGIALRNLEQAAGSVALATAIPQHRGTDMP
                     EWHAMMQVIAEVSRETYRKLVFETPRFYDYFRKATPIDLIERMQIGSRPASRRGGQGV
                     NNLRAIPWVFAWTQSRHILPGWYGVGTGFAAAIDKFGDEAFREMFREWYFMRALIADV
                     EMVLAKADLGIASAYSKLAGDLHEEFFPHIEKGFNLTQDLILSFSGQKALLENDPTLQ
                     RAIMLRNPYVDPMSLMQVTLLERWRQSGYRDAALFEALLASVNGIAQGLQNTG"
BASE COUNT          717 a         1007 c         1131 g          567 t
ORIGIN      
        1 gcgcctgctg gaccgacacc cggatggcag caccgccggc ggtgatgccg ttgccgccgc
       61 cgtaactccg cggcctgcgg agatttgcga tgttgctctc caggtttcgt tactatggcg
      121 ggcccgggag ccggattcgg tacgcgcgaa accgtctcca gggcctgccg ggacaggcgg
      181 accaagcaag aggtgcacga atgccatcat ttgacgtggt gagcgagttc gactcgcacg
      241 aggccagcaa cgccgtcgac caggcgaatc gcgaagtgga tacccgcttc gacttcaagg
      301 gcacgggctc caggtacgtg ctggaagcca atgtcatcac gatgacgagc cagaccgact
      361 tccagctgca gcagatgctc gacatattgc gccagaagct ggcgaagaga ggcatcgaca
      421 tcgcgtgcat gaaagaagag cagccggaaa tttcgggcag cgaggcgcgg cagaagatca
      481 tcctgcgcca gggcatcgac tccgacctgg cgagaaacct ggtcaagaag atcaaggcga
      541 gcaagatcaa ggtgcaggcg gcgatacagg gcgaaaagct cagggtcagc ggcaagaaac
      601 gcgatgatct gcaggccgtg atcgccctgc tgaaggaggc cgacgccggt cttcccctgc
      661 agtacgaaaa ctttcgcgac tgagcgctcc cgccatgccg acagcactgc gcaggcagga
      721 catcacgttc gaagacaagg accagccgct gcgcgatgac gtgcgcacgc tcggcgggct
      781 cgtcggggag ctgctgaagg agcagggcgg cgagaagctt tttgatttcg tcgagaacgc
      841 gcgcgttcaa tccatacgcc gccgcgaggg caatgaaaag ccgggggaga aactcgaaga
      901 tcttttcgac aacctcgatc tcgggttcgc gctggaggtc attcgttctt tctcgaccta
      961 tttccagatg gtgaataccg cggagaaggt gcacaggatc cggcgacgcc gcgaatacct
     1021 gcaggaagaa ggcaagtacc agccgggcgg cctcgaggag acgcttgtaa aactgaaggc
     1081 gggaggagtc gatgtgccac gcctgcaggc gctgctcgac tcgatccgta tcgagcccgt
     1141 attcaccgct catccgaccg agccgacgcg ccgcacgatt ctgcgcaagg agcagaacat
     1201 cgtaaggcag ctggtgaaca tgctgaaccc gacgatgacg gcgcaggaaa agaacgtcgc
     1261 gatccagaat ataaaaatgg aggcgacgac cgggtggcag acgaacgaac acccctcgga
     1321 gcagatgacg gtcgccgatg aactggagca cgtactgttt ttcgtcaccg acgtgctgta
     1381 ccgcactatc ccgccgttct acgaggacct cgagagcgcg gcggtacgca tctacggcga
     1441 agaggcgcgt gacctgcatg tgccgggcct gctgcgcttc gcctcgtggg tcggtggcga
     1501 catggacggc aacccgaatg tcaacgcgaa gacgattcgc gaaaccctgg cgcggcagcg
     1561 ctcgttgata ctcgacctgt attaccgtga atgcggcaag ctttcggcga agctgagcca
     1621 gtcgaagaca cgcgtcggcg tcgaggatgc cgtctacgcc aagatcgagg aatacacccc
     1681 tgcgttccag cacgcgtacc acgcggtgcc tgcgcgtcac cgggacatgc cctaccgggt
     1741 gttcctgcgg ctggtacagc aacgcctcca ggcgacgtat gacgatgacg cgtatcccta
     1801 cgagtcaccc gaggagtttt atgccgacat cgatctgata gcccggagcc tggcggccaa
     1861 caagggagag cacgcgggac tcttcgcggt gcggcgattg ctgcggcgga ttgcaacctt
     1921 cggcttccac ctcgtgacgc tggacgtgcg gcaggacgcc ctcgtacacc gggaggtcgt
     1981 cggagaatgc atcggggacg ataagtggct cgagcgcagc cccgaatacc gggtcgagaa
     2041 gatcgccgag gcaatcctgc aaaaggaccc gcccgtgcta gagcaaagca ccaaggcgcg
     2101 caagacgctg gcggtgttcc aggcgatcgc tttctgccgg cgcaagtacg gtgccgcggc
     2161 catcgggcct ttcatagtca gcatgacgca gggcgtcgac gacatccttt cggtgatcct
     2221 gctgtcggaa tggagcgaac tgcacaaccg ccgcggggag gtgccgctgg atatcgcgcc
     2281 gctgcttgaa acggtggacg acctgaagcg cggtccgcag atcctgcgca cgcttctttc
     2341 gcacgaaatc taccggcagc acctggagca gcgtggcaaa aggcagacgg tgatgatcgg
     2401 gtattcggac agcaacaagg agtccggaat agccgcggcg cggtgggctt tgcaggaagc
     2461 gcagatcgac ctggtgcagg ccgtcgcggc cgaagatgtc gtgctgcagc tgttccacgg
     2521 gcgcggcggg acgatcagcc gtggtggcgg acggacgcac gctgccgtgc tcggcgcgcc
     2581 accgggtacg gtgaacggct acctgcgcgt caccgagcag ggcgagatca tcaacgagaa
     2641 gtacgggttg cgcggcatcg ctttgcgcaa cctcgagcag gcggcgggct ccgtggcgct
     2701 cgcgacggcg ataccgcagc atcgcggcac agatatgccg gagtggcacg cgatgatgca
     2761 agtgatcgcg gaggtcagcc gcgagactta tcgcaagctg gtgttcgaga cgccgcggtt
     2821 ttacgactat ttccgcaagg ccactcccat tgacctgatc gagcgaatgc aaatcgggtc
     2881 ccgcccggct tcgcggcgcg gcggccaggg cgtaaacaac ctgcgtgcta tcccctgggt
     2941 attcgcatgg acgcagagcc gccacatcct gccggggtgg tatggcgttg gaacgggctt
     3001 cgccgctgcg atcgacaagt tcggggacga ggcttttcgc gagatgttcc gcgaatggta
     3061 tttcatgcgg gcgctgatcg ctgatgtcga gatggtcctg gcgaaggcgg acctggggat
     3121 agcaagcgcg tattcgaaac tggcgggcga cctgcacgag gagttcttcc cgcacatcga
     3181 aaagggtttc aacctcacgc aggacctgat cttaagcttt tccggccaga aggcgctgct
     3241 ggaaaatgac ccgacgctgc aacgggcgat catgctgaga aacccttacg tcgacccgat
     3301 gagcctcatg caggtgacac tgctcgagcg ctggcgccag tccggctatc gggatgctgc
     3361 gctgttcgaa gctctgctgg ccagcgtaaa cggcatcgcg cagggcctgc agaacacggg
     3421 tt
//
LOCUS       DASUOM010000146         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_133385,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000146 DASUOM010000000
VERSION     DASUOM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133385"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFY27_02910"
     CDS             complement(<1..425)
                     /locus_tag="VFY27_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5764488.1"
                     /translation="MLSSELEFCLNEAFQNARDRRHEFMTVEHLLLALLDIPKVHEIL
                     KACNSNIGQLRRQLSEFIDEATPLMPLEEEGDVQPTLGFQRVLQRAVFHVQSSGKKEV
                     TGSNVLVAIFSEKQSQAVYFLSLQDITRLDVVNYISHGMP"
     gene            complement(508..837)
                     /gene="clpS"
                     /locus_tag="VFY27_02915"
     CDS             complement(508..837)
                     /gene="clpS"
                     /locus_tag="VFY27_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5764489.1"
                     /translation="MSERKTPKPRTHGYDVAVEEALPKVKRPPLYRVILVNDDYTPME
                     FVVEVLETVFGMERSQATRVMLEVHTRGKGICGVYSYEIAETKVAQVTNIAQQQQHPL
                     LCTMEEA"
     gene            1263..2183
                     /gene="gspC"
                     /locus_tag="VFY27_02920"
     CDS             1263..2183
                     /gene="gspC"
                     /locus_tag="VFY27_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEX5764490.1"
                     /translation="MTIAKWTDFSSLDRERTIAAVNRVAPKWVALALVVLIAWLLARI
                     VWMLVPGPAAGDPVEAEPGVIAQGANAAGEADVQLIANAHIFGEARAVEEAPPPAANH
                     DDLAETRLNLSLKGTITATDERSSIAIIADTGNEEKVYAVSDTVVPGTTLHAVYADRV
                     ILNRSGALEALKLPKEFPQGSQTVRRVAPPPETRAADANPTIQEVVSENVTKLADVIR
                     PTPYFVAGQQQGYRVYPGRDRKQFAALGLRPGDLIKDIDGAALTDPQQAMEIFQNLGS
                     ADQVSVTVERNGQPEVLVLSTSQLSLDNDQ"
BASE COUNT          495 a          681 c          665 g          488 t
ORIGIN      
        1 ggcatgccgt gcgaaatgta gttgacgacg tcgaggcggg tgatgtcctg gaggctcagg
       61 aagtaaaccg cctgcgattg cttttcgctg aagattgcga ccagcacgtt gctgcccgtc
      121 acttccttct tgccgctgga ctgtacatga aaaacagccc gctgcaggac ccgttggaag
      181 ccgagtgtag gctggacatc gccttcttcc tcgagcggca tcaaaggcgt cgcctcatcg
      241 atgaattcgg acagctgtcg ccgcaattgc ccgatattcg aattgcacgc tttcaggatc
      301 tcgtgcactt tggggatgtc gagaagcgcg agtagcaggt gctcgacagt cataaactca
      361 tgacgccggt cccttgcgtt ctgaaacgct tcgttcaggc aaaattcaag ttcgctgctc
      421 aacataaatc aatactcaga aaatcgatct tcaaccgtct caagatacta tgaccactcg
      481 catacctccc ccgttcattt tcgaatttca cgcttcctcc atcgtacaca gaagcgggtg
      541 ctgttgctgc tgtgcgatgt tcgttacctg cgccaccttg gtttcggcga tctcgtagct
      601 gtagacgccg cagataccct tgcccctggt atgaacttcg agcattaccc gggtcgcctg
      661 cgaacgttcc attccgaaca cggtctcaag gacctccacc acgaactcca tgggcgtata
      721 gtcgtcattg acaagtatca cccggtagag cggcggccgc ttgaccttcg gcagcgcttc
      781 ctcgactgcc acatcgtagc catgggtacg cggtttgggc gtttttcgtt cagacatatt
      841 tcactacgtt cttcaataaa tcctgaataa gctcacagta ggtcgcaatt tcctcaattt
      901 ccctgtacat aatcggtgtg cctgggcagg aggctatggc ataagcctga acggctggtc
      961 aggcgggctt aagccggcga ccttttagag acattatata tgggcctcat tccgtgttct
     1021 ccaatgccgc gcgcgcaaaa cgacgccaat gcggggagaa cggcggcccg ctgttaaaac
     1081 cagccttaaa tatagaaggt agctatttgc tgtacaaaga ttttttagcg gtttgggctt
     1141 gtctggcagg gcctgcgaac cgtatcatcc gcaagcgatg ttgactgccg gattgaactt
     1201 ataaaggagc gctccattcg tggcctctgg aggccgcgtg gacgatgcgc cagtctcaaa
     1261 gtatgacgat tgcaaagtgg accgatttct caagcctcga ccgggaacgg accattgcgg
     1321 ccgtaaatcg ggtcgccccg aaatgggtcg cgctggcact ggtcgtgctg atcgcatggc
     1381 tgctcgcgcg catcgtgtgg atgctggtgc cggggccggc cgccggtgat cctgtcgaag
     1441 cggagccggg tgtcatcgcg cagggggcga acgcggcagg cgaggcggac gtgcagttga
     1501 ttgcaaacgc ccacatcttc ggcgaagccc gggctgtcga ggaagcaccg ccgccggcag
     1561 caaaccatga cgatctcgcg gagacccggc tgaacctttc gctcaagggg accatcacgg
     1621 caaccgacga gcgctcctca attgcgatca ttgcggatac ggggaacgag gaaaaagtgt
     1681 acgcggtcag cgacaccgtc gtcccgggaa ccaccctcca tgcggtctat gccgaccggg
     1741 tcattctcaa ccgcagcggt gcactcgagg cgctgaaact gccgaaggag ttcccgcagg
     1801 gctcgcagac ggtccgtcgc gtcgccccgc caccggaaac aagagctgcc gacgccaatc
     1861 caactattca ggaagtggtc tccgagaatg tgacgaagct cgctgatgta atacgtccga
     1921 ccccgtactt tgtggcaggg caacaacagg gctatcgcgt ctacccgggc cgggatcgaa
     1981 aacagtttgc cgcactgggc ctgaggcccg gtgacctgat caaggacatc gacggcgccg
     2041 cgctgacgga tccgcagcag gcgatggaga tcttccagaa tctcggcagc gccgaccagg
     2101 tctcggtgac ggtggaacgg aacggccagc ccgaagtgct tgtactgagc accagccagc
     2161 tcagcctcga caacgaccag tagtgaagaa tttgagaata ccgtacacgt tcgttgccgg
     2221 ctgtcgccgc ttgttgatgg cgaccctcgc ctgtttgctt gccgcaccgc tcgtactggc
     2281 gcagcagcag tcgaccatta cccccaatta caaggacgcg gacatccgg
//
LOCUS       DASUOM010000147         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_991878,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000147 DASUOM010000000
VERSION     DASUOM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991878"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFY27_02925"
     CDS             complement(<1..326)
                     /locus_tag="VFY27_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX5764491.1"
                     /translation="MSTRDLEPATADSWWDAIISRDPAADGSFFYAVRTTGVYCRPTC
                     PSKRPRRANVEFFATGEAAEKAGFRPCKRCSPRAAVQPERHARKVAEACRLIEQSEQA
                     PDLNTLA"
     gene            complement(435..1052)
                     /locus_tag="VFY27_02930"
     CDS             complement(435..1052)
                     /locus_tag="VFY27_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5764492.1"
                     /translation="MIANSETSKTEARTQAQRERILAAAHACFVKHGFHAASMATIAE
                     TAGMSAGLIYRYFENKNAIILSIIERQLADARSDIATLRSDTDFVSLFTDLFAKWQSD
                     DPELMSPALMLEMTAEASRDPQIGAALAESDKVTGGDFNAWLAQSARLEGKKPSAEDL
                     KARAFALRCLIGGLAIRAIREPDVEKTVLQDSLKLMLPHLLSFRT"
     gene            complement(1049..2491)
                     /locus_tag="VFY27_02935"
     CDS             complement(1049..2491)
                     /locus_tag="VFY27_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX5764493.1"
                     /translation="MKGAKPVLAHKRNLVVLTALLGAAGCGILEPALPEADPQIAAEW
                     PIPPTTIETPATSPVEALRAGEISEIGWRDFFTDPNLEQLIAFSLDHNRDLRVAVLNV
                     ERARALYRIQRSDRVPSLNARALMNRGGGENVFLTEAHAVELVVPAFELDLFGRVRNL
                     SEAALRDYLAQEEAQRATQLSLIAEVANAYLTLVSDRELQEVAEAALKNRQEELSLTE
                     RRFELGAASSLEVNQAQIEVETARSDVWQFAGQVAQDINALTLLTGTTVDPALLSTSG
                     VDMQASGVGALPAGLPSDVLLRRPDVQAAEFRLRSANALIGAARAAFFPSITLTGSFG
                     TASNDLSDLFESGTDAWNFIPQIAQPIFQGGRLRANADVANVDRDIALADYERTIQAS
                     FREVADTLALTQTLARQRESREALLQAASRANELSRIRYEAGSDSYLVLLDAQRTLYA
                     AQQALVSTRLAEQANRVNLYKALGGGWNEQ"
     gene            complement(2460..>4096)
                     /locus_tag="VFY27_02940"
     CDS             complement(2460..>4096)
                     /locus_tag="VFY27_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5764494.1"
                     /translation="FFGWFNRNFDRTAQKYRQGVRGMISRSGRFMAVFGVLVVLMLVV
                     FLNRPSSFLPQEDQGVLFSMVLAPVGATQERTMESVIKLEEHFLNNEPEAVESLFTVQ
                     GFSFGGSGQNNAMAFIKLKPWEERESDDLSIDAIAGRAMAALSQIKDAMVFAFGPPAI
                     PELGTSAGFVFYLKDNANLGHDALVGAANQFLGAAAQNPTMVNVRHNGQPDAPQFQLD
                     LDIEKATALGLSVGDINATLQAAWGGQYIDDFIDRGRVKRVFIQADAPFRMVPEDFRL
                     WSVRNDQGEMVPFTAFASSHWDYGSPRLERYNGAPAMEINGEAAPGVSSGDAMAEVER
                     LVSELPQGIGLEWTGLSYQEREAGAQTPLLYTLSLLIVFLCLAALYESWTIPTSVLMV
                     APLGILGIALAATLRGLDRDIYFQVAMLTTIGLTSKNAILIIAFAKDNLDEGMELVEA
                     TMQAVRTRLRPILMTSLAFGMGVLPLALATGAGSGAQRAIGTGVLGGMIAGAFLGIFF
                     VPLFFVVVRRLFTRRERIEEEQPEMIGEGSEAGAGA"
BASE COUNT          764 a         1280 c         1273 g          779 t
ORIGIN      
        1 gccagcgtgt tcaggtccgg cgcctgctcc gactgctcga tcagcctgca cgcttcagca
       61 accttacggg catgccgttc gggttgtacc gccgcccgcg ggctgcagcg tttacagggg
      121 cgaaagcctg ctttctcggc agcttcgccg gtggcgaaaa actcgacatt cgcgcgccgc
      181 ggccgcttcg acgggcaggt gggccggcag tagacgccgg tggtcctgac ggcgtagaaa
      241 aagctgccgt cggccgcggg gtcgcgcgag atgatcgcat cccaccatga atcggctgtt
      301 gcgggttcga gatcgcgggt gctcatgtac atatcctgat cctcctgaat tcgacttgca
      361 cgcaatctat cgaaggcgga ggagggcgaa tatccggttc ttgcggtcga atccggggga
      421 ttttattttt acgatcaggt gcggaaggag aggagatgcg gcagcatcag cttcaggctg
      481 tcctgcaaca ctgttttctc tacatccggt tcgcggatcg cgcggatggc gaggcccccg
      541 atcaggcatc gcagcgcgaa cgcccgcgcc ttcaggtctt ccgccgacgg cttttttccc
      601 tcgagccgag ccgactgcgc aagccaggcg ttgaaatcgc cgccggtcac cttgtcggat
      661 tcggccagtg ccgccccaat ctgcgggtcg cggcttgcct ctgcagtcat ttccagcatc
      721 agcgccgggc tcatgagttc gggatcgtca ctctgccact tggcgaagag atcggtgaac
      781 agcgaaacga aatccgtgtc ggatcgcagc gtcgcaatgt cggaacgcgc gtccgccagt
      841 tgccgctcga tgatggagag gatgatcgcg ttcttattct caaagtatcg gtatatgagt
      901 cccgcgctca tgccggcagt ctcggcgatc gtcgccatgc tcgcggcgtg gaaaccgtgc
      961 ttgacgaaac aggcgtgcgc ggcggcaaga atcctttccc gctgcgcctg cgtacgggcc
     1021 tcagtcttcg aagtttcgga gttcgcgatc actgttcgtt ccagccgccg cccaacgcct
     1081 tatacaggtt cacccggttc gcctgctccg cgaggcgggt ggagaccagg gcttgctgcg
     1141 ccgcgtaaag cgtccgctgt gcatccagca gtacgagata actgtcgctg ccagcctcat
     1201 agcgaatgcg ggatagttcg ttggcacgcg aagcggcttg aagcagcgct tcgcgcgact
     1261 ccctctggcg cgccagcgtc tgcgtgagcg cgagcgtatc ggcgacttcg cggaagctcg
     1321 cctggattgt ccgctcgtaa tctgcaagcg cgatatcccg gtcgacattg gccacgtcgg
     1381 cgttagcacg caggcgcccg ccctggaaaa tcggttgcgc gatctgcgga atgaagttcc
     1441 aggcgtccgt tccgctttcg aacagatccg acaggtcgtt actggcagta ccgaaactgc
     1501 cggtcagcgt aattgacggg aaaaacgcgg cgcgtgccgc gccgatcagc gcattcgcgg
     1561 accgcaatcg aaattctgca gcctgtacat ccgggcgccg caatagcacg tcggagggta
     1621 atcctgcggg aagagctccg actccgctcg cctgcatgtc gacgccggag gttgacagca
     1681 gtgccggatc gaccgtcgtg ccggtcagca acgtcaatgc gttgatgtcc tgagctacct
     1741 ggccggcgaa ctgccaaacg tcgctgcgtg ccgtttcgac ttcgatctgc gcctggttta
     1801 cttcgaggct ggaggcggcg cctagctcaa agcgccgctc tgtcaaagac agttcctcct
     1861 gccggttctt caacgccgcc tctgcaacct cttgcagctc ccgatccgag accagcgtca
     1921 ggtacgcgtt ggcgacttcc gcgatcaggc taagctgcgt ggcacgctga gcttcctctt
     1981 gcgcgagata atcccgcaag gcagcttcgc tcaggttgcg aacgcgcccg aacaaatcca
     2041 attcgaacgc aggtacgacg agctcgacag cgtgggcttc cgtcaggaag acgttttcgc
     2101 cgccgccacg attcatcaag gcccgggcgt tcagcgacgg cacgcggtcc gagcgctgga
     2161 tccggtacag cgcgcgagcg cgctccacgt tcagcaccgc cacgcgcaga tcgcgattgt
     2221 gatcgagcga gaatgcgatc agttgctcga ggttgggatc agtgaagaag tcgcgccacc
     2281 cgatctcgga aatttcgcct gcgcgaaggg cttccacggg tgatgtggcc ggagtttcga
     2341 tagtcgtggg cggtatgggc cattcggcag cgatctgcgg atcggcctcg gggagcgccg
     2401 gctcaaggat cccgcagccg gcggcaccga gcagcgctgt cagcacgacc aggttacgct
     2461 tatgcgccag caccggcttc gctcccttca ccgatcatct ccggctgttc ttcctcgata
     2521 cgctctcgcc gggtgaagag acgccgcacc accacgaaaa acagcggcac gaagaatatg
     2581 ccgaggaatg cgccggcaat catgccgccg agaacgcccg tgccgatcgc ccgctgagcg
     2641 cccgaaccgg ctcctgttgc gagcgcaagc ggcagcacgc ccatgccgaa ggccagcgac
     2701 gtcatcagga tcgggcgcag ccgggtgcgc actgcctgca tcgtagcttc gaccagttcc
     2761 atgccctcgt cgaggttgtc cttggcaaat gcgatgatca ggatcgcgtt cttgctggtg
     2821 agaccaatcg tcgtcagcat cgccacctgg aaatagatgt cgcggtcgag acccctgagc
     2881 gttgccgcaa gagcaatccc caggataccg agcggggcca ccatcaggac ggaggtcggg
     2941 atggtccagc tttcgtataa cgccgcaaga caaagaaaca cgatcagcag cgacagggtg
     3001 tagaggagcg gcgtctgcgc gccggcctcg cgttcctggt aagacaggcc ggtccattca
     3061 aggccgatgc cttgcggcag ttccgaaacc agccgctcga cttcggccat tgcatcgccg
     3121 gagctgacgc cgggtgcggc ttcgccgttg atctccattg ccggggcgcc gttatagcgc
     3181 tcgaggcgcg gcgaaccgta gtcccaatgc gagctggcga acgcagtgaa gggaaccatt
     3241 tcgccctggt cgtttcgcac cgaccatagc ctgaaatcct ccggaaccat gcggaacggc
     3301 gcatcggcct ggatgaagac ccgtttgacg cggccacggt caatgaagtc atcgatgtac
     3361 tgtccgcccc acgccgcctg caaagtagcg ttaatgtcac ccacggaaag accgagcgcg
     3421 gtcgctttct cgatgtcgag gtcgagctgg aactgcgggg cgtcgggctg accgttgtgc
     3481 cgcacgttga ccatcgtcgg gttctgcgca gccgccccga ggaactggtt tgccgccccg
     3541 accagcgcat cgtgccccag attcgcattg tccttgaggt agaaaacaaa cccggcggat
     3601 gtaccgagtt ccggaatggc cggagggccg aaagcgaaca ccatcgcgtc cttgatctgg
     3661 ctcagagctg ccatcgcgcg acccgcgatc gcgtcgatgc tgagatcgtc tgattcgcgt
     3721 tcctcccatg gctttagctt gatgaaggcc atggcgttgt tctggccact gccaccgaag
     3781 ctgaacccct ggacggtaaa cagcgactcg accgcctcgg gttcgttatt caggaaatgt
     3841 tcttcgagct tgatgaccga ttccatggtg cgttcctggg tcgcgccgac gggcgcgagg
     3901 accatggaaa acagcacgcc ctggtcttcc tgcggcagga acgaacttgg ccggttcagg
     3961 aacacgacga gcatcagtac gacaagcacg ccgaagacgg ccatgaagcg gccgctgcgg
     4021 gagatcatgc cccgcacccc ctggcggtat ttttgtgcag tgcggtcgaa gttgcggttg
     4081 aaccagccga aaaatc
//
LOCUS       DASUOM010000148         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_839316,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000148 DASUOM010000000
VERSION     DASUOM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839316"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFY27_02945"
     CDS             complement(<1..974)
                     /locus_tag="VFY27_02945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5764495.1"
                     /translation="MKISIIGLSLSSSWGNGHATTYRSLIKGLKRRGHEVSFYERSES
                     WYAAHRDLKRASYLHFYSTAAELLYEWREELEASDLVIIGSYVLESSMITREIKPSGA
                     LLAFYDIDTPVTIAQIRNDRCAYLERDLLPEFDLYLSFSGGTVLDELRDLGAQRPVAF
                     YCSVDPDVHAPLEVPVSIDLGYLGTWSEDRQAPLTELLLEPAQRWNGGRFAVAGPQYP
                     DADEWPQNVHHVSHLPPHEHAGFYCSQRFTLNLTRADMRRMGYSPSVRLFEAACCGTP
                     VISDDWCGLDEVFRAGKEILIAHDGDDVLEYVRDMDEDERAAIGAAARS"
     gene            complement(982..2073)
                     /locus_tag="VFY27_02950"
     CDS             complement(982..2073)
                     /locus_tag="VFY27_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5764496.1"
                     /translation="MSLDISFFVSSILSAYWNGAATYYRGLARGLSERGHRLHFFEPD
                     AFDRQTHRDLDELPWGDVTVYAPDDWQQMKECLRIAGQSDFAVKAGGIGVNDELLEKE
                     VAALRSAGTGALFWDVDAPATLARLEGNADDPFHKVLKDYDAILCYGGGENVRSRYLA
                     LNARRCEIVYNALDPASHFPVEPDRRFTGDLGFLGNRLPDRERRVEAFFFAPARRLSS
                     RQFVLGGSGWDSTASSLPNLRYVGHVYTTDHNAFNMSPKAVINISRDDMAEAGFSPAT
                     RVFEAAGSGSCLITDYWEGIELFLEPGEEVLVAHDGEDVASWVEELSPERAKAIGRRA
                     RERVLSGHTYGHRAEQLEVLLCGYGSGGV"
     gene            complement(2070..3188)
                     /locus_tag="VFY27_02955"
     CDS             complement(2070..3188)
                     /locus_tag="VFY27_02955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5764497.1"
                     /translation="MKFVLFYHSLTSDWNHGNAHFLRGVATELILRGHDVRVYEPADG
                     WSLAQLLQTEGQQAVEGFSHHYPLLRPIRYKLETLDLHKALDNAHVVVVHEWNPVELL
                     ERIGRHRRSSRDRYQLLFHDTHHRAVSAPRELARYPLEDFDGTLAFGEAIADIYRRRG
                     WGGRVWIWHEAADTRLFKPAERPTAAEAELVWIGNWGDEERSAELRTFLFEPVRKLGI
                     SGSVYGVRYPKQALDTLAHSGLHYRGWVPNYSVPSVFHCHRFTVHVPRKFYRTKLPGI
                     PTIRVFEALACGIPLLSAPWSDAENLFRAGRDFVMVRDGAAMQKAMRNLLADRAMADE
                     IAMSGRATIEQRHTCAHRVDELIAIVHELKDVPEAALS"
BASE COUNT          632 a         1034 c         1008 g          633 t
ORIGIN      
        1 gaccgcgcgg ccgcaccgat ggcagcccgc tcgtcttcat ccatatccct cacatactcc
       61 agcacgtcat ccccgtcgtg cgcgatcagt atttccttgc cggcgcggaa cacctcgtcg
      121 agcccgcacc agtcgtccga aatgaccggc gtgccgcagc aagcggcttc gaacagccgc
      181 acactgggcg aataacccat gcgccgcatg tcggcgcgcg tcaggttcag cgtgaagcgc
      241 tggctgcagt aaaagccagc gtgttcgtga ggaggcaggt gcgacacgtg atgcacgttc
      301 tgtggccact cgtcagcatc aggatactgc ggacccgcga ccgcaaatcg cccgccgttc
      361 caccgttgtg ccggctcgag caggagttcc gtcagcggtg cctgccggtc ctcggaccat
      421 gttccgaggt agccgagatc gatcgacacc ggaacctcga gcggcgcatg cacgtccgga
      481 tcgacggaac aatagaaagc taccggccgc tgtgctccca ggtcgcgcag ttcatcgaga
      541 acagtgccgc cggaaaagct caggtaaagg tcgaactccg gcagcaggtc gcgctcgagg
      601 taagcgcacc gatcgtttct gatttgcgcg attgtcacgg gagtgtcgat gtcgtagaaa
      661 gccagcagcg ccccggaagg tttgatttcg cgggtgatca tcgatgattc gagcacatag
      721 gacccgatga tgacgaggtc ggatgcttcc agttcttcgc gccattcgta cagcagttct
      781 gcggcggtgc tgtagaagtg gaggtagctc gcgcgtttga gatcccgatg cgctgcatac
      841 cagctttcgc tgcgttcata gaaggacacc tcgtgtcccc tgcgcttaag tcccttgatc
      901 agcgaacggt aggtcgtcgc atggccgttt ccccacgagg agctgagact caagccgatg
      961 atggaaatct tcatactttc gtcaaacgcc tccagagccg tatccgcaca acagcacttc
     1021 gagctgttcc gcgcgatgac cgtaggtgtg ccccgacagt acgcgctcgc gcgcgcgcct
     1081 gccgatcgct tttgctctct ccgggctcaa ctcctcaacc cagctcgcga cgtcctcgcc
     1141 gtcatgggcc accaggactt cctcgccagg ttcaaggaaa agctctattc cttcccagta
     1201 atcggtgatg aggcagcttc cggagccggc tgcttcgaat acacgggtag cgggggagaa
     1261 acctgcctcg gccatgtcgt cacggctgat gttgataact gccttcgggc tcatgttgaa
     1321 ggcgttgtga tccgtcgtgt agacatggcc tacgtagcgc aggttgggca gcgaagaggc
     1381 cgtggaatcc catcccgagc cgcccagcac gaactggcgg ctcgagagtc tgcgcgccgg
     1441 ggcgaaaaag aacgcttcta cccgtcgttc ccggtccggc agtcggttgc cgaggaagcc
     1501 gaggtcgccg gtaaaacgcc tgtcgggttc gaccgggaaa tgcgaggcag ggtcgagggc
     1561 gttgtagacg atctcgcagc ggcgcgcatt caacgcaagg taccgtgacc gcacgttttc
     1621 gccgcctccg tagcaaagga tggcgtcgta gtctttcaat accttgtgga aagggtcgtc
     1681 ggcgttgcct tccagccgcg caagcgttgc cggcgcatca acgtcccaga acagcgctcc
     1741 cgtgcctgcc gatctcaatg ccgccacttc cttttcgagc agctcatcgt ttacaccgat
     1801 gccgccagcc ttgactgcga aatcgctttg ccccgcaatc cgcagacatt ccttcatctg
     1861 ttgccagtca tccggcgcat aaacggtgac atcgccccat ggcagttcgt cgagatcgcg
     1921 gtgggtctgc cgatcgaacg catccggttc gaagaaatgc aggcgatgcc ctcgctctga
     1981 cagcccgcgc gcgagacccc ggtaataagt cgctgcgccg ttccagtagg ccgacaggat
     2041 gctggagacg aaaaagctga tgtcgagact catgacaatg ccgcctcggg cacatcttta
     2101 agttcatgga cgattgcgat aagttcgtca acccgatgcg cgcatgtatg ccgctgctct
     2161 atggtcgcgc ggccgctcat cgcgatctca tccgccatag cgcggtcggc aaggagattc
     2221 cgcatggcct tctgcatggc ggcgccgtcg cgcaccatca cgaaatcgcg tcccgccctg
     2281 aagagatttt ccgcgtcgct ccacggcgcc gagagcaggg gaattccgca agccaacgct
     2341 tcgaatacgc gaatcgtcgg gatgcccggg agtttggttc ggtagaactt tcgcggaacg
     2401 tgaacagtga aacggtggca gtggaacact gacggcaccg agtagtttgg tacccagcct
     2461 cggtagtgca ggccggaatg cgccagcgtg tcgagcgcct gcttcggata acgcacgccg
     2521 tagactgacc cgctgatgcc gagctttcga accggctcga acaggaaagt gcgcaattct
     2581 gcgctgcgtt cctcatcgcc ccagttgccg atccagacca gctcggcctc ggctgcggtc
     2641 ggccgttccg ccggcttgaa gaggcgggta tcggctgcct cgtgccagat ccagacgcgg
     2701 ccaccccagc ccctgcggcg gtagatgtcg gcgatggcct caccaaacgc cagggtgccg
     2761 tcgaaatcct caagcgggta acgcgcaagc tcgcgcggtg cgctgacggc gcggtgatga
     2821 gtgtcatgga atagcagctg gtagcgatcg cggctgctcc gcctgtgccg gccgatgcgc
     2881 tcgagtagtt cgacggggtt ccactcgtgc acgacaacga cgtgcgcgtt atcgagtgcc
     2941 ttgtggaggt ccagtgtctc cagcttgtaa cgaatcgggc gaagcagcgg atagtgatgc
     3001 gaaaagccct cgacagcctg ctgtccctcc gtttgcagca actgtgcgag gctccagccg
     3061 tctgccggct cgtatacacg cacgtcgtgc ccgcgcagga tcagctccgt cgcgacgcca
     3121 cgaaggaaat gggcgttgcc gtgattccag tcggatgtga gcgagtgata aaagagaacg
     3181 aacttcacgc gacaccccgc gccggaacgt ccttaaggtt ccggtattgc tccgagtagg
     3241 ccgcagccat agcggttgcc gggtaacggg ccgcgcgcgc gcgagcactt tcgcccagtc
     3301 gccggcg
//
LOCUS       DASUOM010000149         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1596917,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000149 DASUOM010000000
VERSION     DASUOM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596917"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFY27_02960"
     CDS             <1..827
                     /locus_tag="VFY27_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764498.1"
                     /translation="ILALPVMAAAQEEDLSTIPWAYSTYFGTGWYKIGEERDAFAIRY
                     APRKPMREAVLHEDGSRTIGIELRLPLTVGLDHFPLDDLQGSVDPANLANISITPSIY
                     FDVPMNERWTLRPFVAAGWGTVLNGDESAWTYWTGLRSRLLFKLGKVDAALLNSIGFV
                     GYTPNVGSSTNFWPLLTAVEMATPLGDLQIDERQLLLHWHLGYTYFTDNLDIVRRDLT
                     TEPISDQWELGLAVSKVDAQIQVWRFKFDRVGLAYRFSSDGKLQGVGFVFRSLFDQ"
     gene            complement(918..1688)
                     /locus_tag="VFY27_02965"
     CDS             complement(918..1688)
                     /locus_tag="VFY27_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5764499.1"
                     /translation="MPALNVTIVQSDLHWHEPARNLEHFSSLLRSNTQGTDLVVLPEM
                     FSTGFTMHAAPHAESMDGRSVTWMRETARERDIAICGSLIIEDDGRYFNRFLLVRADG
                     TTEYYDKRHLFRLAGEHEHYAAGPSVRTFELKDWRIRPMVCYDLRFPVWSRNRNDYDL
                     LVYVANWPDRRHQAWEALLRARAIENLAYVAGVNRIGTDGNDIRYVGGSAVIDYLGKY
                     LSSPGEACGVFSATLDMAGLLEFRERFPFHLDADEFTL"
     gene            complement(1676..2875)
                     /locus_tag="VFY27_02970"
     CDS             complement(1676..2875)
                     /locus_tag="VFY27_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEX5764500.1"
                     /translation="MVAVTVRMKALGTRMSQPLQSKLPAVGTTIFTIMSQLAADCGAI
                     NLSQGFPSFDPDPELKRLIYHHLRSGANQYAPMPGVPSLRQEIAAKVSRLYGRTVDES
                     TDVTVCTGATEGLFSTIQAMVRPGDEVIVLDPAYDSYEPAVTLAGGRTCHVPLIEVDA
                     SFRIDWARLRDTISKKTRLIILNIPQNPTGAVLGGDDLETLAEILRDTSAFLVSDEVY
                     EHIIFDGVAHQSLLRHDELWERSFVISSFGKTYHATGWKVGYVIAPTMLTTEFRKVHQ
                     FTTFSVTTPIQHAIADYMARTPEHYDTLPAFYEAKRDRFCALVKDSRFRFRPAQGTFF
                     QILDYSGITDEPDLAYAKRLTREIGVASIPVSVFCEEPPPGRKLRFCFAKDDATLEEA
                     AAKLCQL"
     gene            complement(2851..>3341)
                     /locus_tag="VFY27_02975"
     CDS             complement(2851..>3341)
                     /locus_tag="VFY27_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764501.1"
                     /translation="DGSRLLFGGNPLKNHSIPDRYGAIEPTAVQVPLEALCGEYFELV
                     TTELFGPFQVIVKYRDVDLPLVLQSLERMSQHLTAAVVSADIAFQNRVLAETINGTTY
                     CGMRARTTGAPQNHWFGPCGDPRAAGIGTPEAIINTWSGHREIIHDQGPLPEEWSPSP
                     FA"
BASE COUNT          651 a          974 c         1024 g          692 t
ORIGIN      
        1 taatacttgc gctgccggta atggccgctg cgcaggagga ggacctgagt actatcccct
       61 gggcgtactc gacctacttc ggcaccggct ggtacaagat cggggaagag cgggatgcgt
      121 tcgcgattcg gtatgccccg cgcaagccga tgcgggaagc tgttcttcat gaagatggct
      181 cgcgaactat cgggatcgaa ctgaggcttc cgctcaccgt agggctcgat cattttccgc
      241 tggatgatct gcagggttcc gtggacccgg cgaacctcgc gaacatcagc ataacgccga
      301 gcatctactt cgacgtacca atgaacgagc gctggactct gcggccattc gtcgctgccg
      361 gctggggaac ggtgctgaac ggcgatgagt ctgcgtggac ctactggaca ggcttgcgca
      421 gcagacttct cttcaagctc ggcaaagtgg acgccgcact tctcaattcc atcggtttcg
      481 ttggctacac acctaacgtt ggctcatcca cgaacttctg gcccctcctg acagccgtcg
      541 agatggccac gccacttgga gatttgcaga tcgatgagcg gcaactgttg ctgcactggc
      601 acctcggtta cacgtacttt accgacaatc tcgacatcgt gcggcgcgac ctgacgacgg
      661 agccgatcag cgatcaatgg gaattgggcc tggcggtgag caaggtggat gcgcaaatcc
      721 aggtatggcg attcaagttc gaccgggtgg ggttggcgta tcgtttcagc tcggacggaa
      781 agctgcaggg agtcggcttc gtgttccggt cgttgtttga ccagtagcag gaaggacgga
      841 gcttgcggca ggcagtcgcc cgcggggcgg gctactacag cctgcggaga atgctggtaa
      901 gcagagtggg caacttgcta cagggtgaat tcgtcggcgt ccaggtgaaa tggaaatctc
      961 tcccggaatt cgagcagccc tgccatgtcg agtgtcgcgg agaaaactcc gcaagcctct
     1021 cccggagagg agaggtattt gcccaggtaa tcgataacgg cgctaccgcc gacatagcga
     1081 atgtcgttgc catcggtgcc aattcggttg acgccggcga cataggcgag gttttcgatc
     1141 gcgcgcgccc gaagcagcgc ctcccatgcc tggtggcggc ggtccggcca gttcgcaacg
     1201 tagaccagca ggtcgtaatc gttgcggtta cggctccaca ccggaaatcg caggtcatag
     1261 cagaccattg ggcggatacg ccagtccttc agctcgaagg tccgcacgga tggtcccgct
     1321 gcgtaatgct catgctcgcc agcgaggcgg aacaggtgcc gcttgtcgta atattctgtc
     1381 gtgccgtcgg cgcgcacgag caggaagcgg ttgaagtagc gcccatcgtc ctcgatgatc
     1441 aggctgccgc agattgcgat gtcgcgctcc ctcgccgtct cgcgcatcca cgtcacagaa
     1501 cgcccgtcca tgctctctgc atgcggtgcg gcgtgcatcg tgaatccggt cgagaacatt
     1561 tccggcagca cgaccaggtc tgtcccttgc gtgtttgagc gaagcaggct ggaaaaatgt
     1621 tccaggtttc gcgccggttc gtgccagtgc aggtccgact gcacgatcgt cacattcaaa
     1681 gctggcatag tttcgctgcc gcctcctcga gtgtcgcgtc atccttcgca aagcaaaagc
     1741 gcagcttccg acccggcggt ggctcctcgc agaacaccga caccgggatc gaggcaaccc
     1801 cgatttctcg cgtcagcctt tttgcgtatg cgagatctgg ctcgtcggta atcccggaat
     1861 agtcaagtat ctggaaaaac gttccctgtg caggtcgaaa gcggaagcgt gaatccttca
     1921 cgagcgcgca gaatcggtcc cgctttgctt cgtaaaatgc cggcagcgtg tcgtaatgct
     1981 ccggcgtcct tgccatatag tctgctattg cgtgctggat cggggtcgtc actgaaaacg
     2041 tagtgaactg gtgaaccttg cgaaactctg ttgtaagcat ggtcggcgcg atgacgtagc
     2101 ccaccttcca gccggtcgcg tgataggtct tgccgaaaga ggagattacg aaggaccgct
     2161 cccaaagttc atcatgccgc agcaagctct ggtgtgcgac cccgtcgaaa atgatgtgct
     2221 cgtaaacctc gtccgacacc aggaaagccg acgtatcccg aagaatttcc gcaagcgtct
     2281 ccaggtcgtc cccgccgagg acagctccag tcgggttttg cgggatgttg agaataatga
     2341 ggcgagtctt cttgctgatg gtgtccctca accgtgccca gtcaatgcga aacgacgcat
     2401 cgacttcaat aaggggcaca tgacatgtgc gaccgccggc gagcgtgact gcaggctcgt
     2461 aggagtcata cgccgggtcg agcacgatca cctcgtcacc cggccgtacc atcgcctgga
     2521 tcgtgctgaa tagcccttcg gtagcgccgg tgcacaccgt tacgtccgta ctttcatcca
     2581 ccgtccggcc atacagccgc gataccttgg cggctatttc ctgccgcagc gaaggcacgc
     2641 ccggcatcgg cgcgtactgg ttggcgccgc tacgcaggtg atgatagatc aggcgcttga
     2701 gctcagggtc cggatcgaag ctcggaaagc cctgcgacag gttgatggcg ccgcagtcgg
     2761 cggcaagctg gctcatgatg gtaaatatcg tggtaccgac ggccggcagc ttgctttgca
     2821 gcggttgcga catccgcgtc ccgagcgcct tcatgcgaac ggtgacggcg accattcttc
     2881 cggcagcggc ccctggtcgt gaatgatttc gcgatgtccg ctccaggtat tgatgatcgc
     2941 ctccggagtg ccgatccccg cggcacgcgg gtctccacaa gggccgaacc agtggttttg
     3001 cggcgccccg gtggttcttg cccgcattcc acaataggtg gtgccgttta tcgtttctgc
     3061 gagtacgcgg ttctggaatg cgatgtcggc actgacaacg gcggcagtta agtgttggga
     3121 catcctctcc agcgactgca gtacgagcgg caggtccacg tcgcggtact ttacgattac
     3181 ctggaacggc ccgaacaact ctgtcgtcac gagctcgaag tactcgccgc agagggcctc
     3241 caggggtacc tgcaccgctg taggttcgat cgcgccgtag cggtccggga tggaatggtt
     3301 cttcagggga ttgccgccga agaggagcct ggaaccgtct a
//
LOCUS       DASUOM010000150         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1113270,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000150 DASUOM010000000
VERSION     DASUOM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113270"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFY27_02980"
     CDS             <1..377
                     /locus_tag="VFY27_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764502.1"
                     /translation="RDRPGAAEVLIDAYAVAGDVETLTALATDGSDPERQAQAIRALG
                     IAGGSEVGSVLSDIYRNSGEVKVREAALEGLLISDDDQAVLALFRESDDAAERKELLQ
                     TLVMMDSDAVWDIIDATLENGE"
     gene            374..1372
                     /locus_tag="VFY27_02985"
     CDS             374..1372
                     /locus_tag="VFY27_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5764503.1"
                     /translation="MTAVRASRLVTAVIVTMIMPGVPAERAMAAGLDALAVDGLYTWR
                     VAATDDAPCWCCVNWSGKSHTARPCDLDSRNVGYSVDDSLDTGGEMQVYALIESGKPT
                     RIRTLSPQCAVSSRRDIIDLGVIGPAESLAWLQERVMSSPVGSDALAAIAVHRDPAAL
                     SFLLDIARAGPAMELREDAIFWMAQGRISESAGELEKLMFRDQDPEIRQHAAFSLSQS
                     SAPNRGDALIRQGREDEDPEVRSQAWFWLAQTGLNGSEKEIRWAIANDPEDDVREEAV
                     FALSQLPGERGVDALFEVLRDQGLHREIREQALFWLAQSDSDRAFAYLDELLAGRK"
     gene            1533..1931
                     /locus_tag="VFY27_02990"
     CDS             1533..1931
                     /locus_tag="VFY27_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764504.1"
                     /translation="MQKLLTGCGLLLSLSLLAGCATTTAGLGEEAGNRSLPSGLTLLD
                     SDEGECDGAVQVGEEMIEDEGEGLDDSFLVEAGQNATFELESGHEDIEWACVDDDSSE
                     IESLECPRDASHVRITRATDGGELLFECYG"
     gene            1919..>2287
                     /locus_tag="VFY27_02995"
     CDS             1919..>2287
                     /locus_tag="VFY27_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764505.1"
                     /translation="MLRLRETPLRHGLLNALTFAVFMTPACSLADFVDVANTVRSRGC
                     ETLAAVDQPLRAQARLDEAARRLARGDELETATSQSGYRAKKSASIRIRTDNGDDAVA
                     EVLAQHFCDIVADKSLLEAGS"
BASE COUNT          441 a          669 c          770 g          407 t
ORIGIN      
        1 tgcgcgaccg tccgggagct gccgaggtcc tgatcgatgc gtacgcggtg gcgggagatg
       61 tggagacact gaccgccctg gccaccgacg gcagcgatcc cgagcgccag gcacaggcga
      121 tccgggctct cggcatcgcc ggcggcagcg aagtgggcag cgtattgagc gacatttacc
      181 ggaactccgg cgaggtgaag gtcagggaag ccgcgctcga agggctgctg atctcggatg
      241 acgaccaggc ggtgctcgcg ctttttcgcg agagcgacga tgcggcggaa cgaaaggaat
      301 tgctccagac gctggtgatg atggacagcg acgccgtctg ggacatcatc gacgccacac
      361 tggagaatgg cgaatgacag cggtccgggc cagccggctc gtgacagcgg tcatagtgac
      421 gatgatcatg ccgggcgtcc ctgccgaacg tgccatggcc gcgggcctcg atgcactggc
      481 tgtcgacggc ctttatacat ggcgtgtcgc ggcgacggac gacgcgccct gctggtgctg
      541 cgtaaactgg tccggcaaat cgcataccgc ccgcccgtgc gatctcgaca gccgcaacgt
      601 cggctacagt gtcgatgaca gcctggacac gggaggagag atgcaggtct atgcgctgat
      661 cgaatccggc aagcccactc gcatccgtac gctcagtccg cagtgcgccg tctcgtcacg
      721 gcgtgacatc atcgatctcg gcgttatcgg gccagcggaa agcctggcgt ggctccagga
      781 acgcgtcatg tcctcgccag tcggttcgga tgcactcgcc gccatcgcag ttcaccggga
      841 tccggcagcg ctgagcttcc tgctcgacat cgccagggcc ggacctgcga tggaattgcg
      901 cgaggacgct atcttctgga tggcacaggg acgaatatcg gagtccgccg gcgaactgga
      961 aaaattgatg ttccgggatc aggacccgga aatccggcag cacgcggcgt tctcgctgtc
     1021 gcagtcgagc gcgccgaatc gcggcgacgc cctcattcgc cagggccgcg aagacgaaga
     1081 tcccgaggtt cgctcacaag cctggttctg gctggctcaa acggggttga acgggagcga
     1141 gaaagaaatc cggtgggcga tcgccaacga tcccgaggac gacgtgcgtg aagaggctgt
     1201 attcgcgctg tcgcagttgc cgggagagcg cggtgtcgac gccttgttcg aggtgctgcg
     1261 agaccagggc ctgcatcgcg aaatccgcga acaggcgctg ttctggcttg cgcaatcgga
     1321 ttcggaccgg gcattcgcct acctggacga actgctcgcc ggccggaagt agccgtaccc
     1381 tttcttgttt ctggtggtcg agcacggatt ggccttctct ggcacggttc ttgctgcaac
     1441 ctggcaggag gacatccgat gcgtccacga gacgcgcaca tggacgtcat tctgcaggac
     1501 gacgaaaaac caccaaatcc aaggagtttg atatgcagaa gttattgact ggttgcggat
     1561 tgttgttatc cctttcgttg ctggcaggtt gtgctaccac gaccgccggt ctcggcgaag
     1621 aagccggtaa ccggagcctt ccatccggct tgacactgtt ggattcggat gagggcgagt
     1681 gtgacggtgc cgtgcaggta ggcgaggaaa tgatcgagga cgaaggggaa ggcctcgacg
     1741 actccttcct ggtcgaagcg ggccagaatg ccaccttcga actggaaagc gggcatgaag
     1801 acatcgagtg ggcatgcgtc gacgatgatt cctcggagat cgagagcctg gaatgcccgc
     1861 gtgacgcttc gcacgtgcgc atcacgcgtg ccacggatgg aggcgagttg ctgttcgagt
     1921 gctacggctg agagagacgc ctctgcgcca tggactgctg aacgcgctga ctttcgcagt
     1981 cttcatgaca ccggcgtgca gccttgccga cttcgtcgat gtcgcgaata cggtccgctc
     2041 ccggggatgc gaaaccctgg ctgccgtcga ccagcctttg cgggcgcagg cacgcctgga
     2101 cgaggcggcg cgacgccttg cgcggggcga tgaactggaa acggcgacgt cgcaatccgg
     2161 ttaccgcgcc aaaaagtcgg cctcgatacg catccgaacc gacaacgggg atgatgccgt
     2221 ggcggaggta ctcgcgcagc acttctgcga catcgtggcg gacaagagct tgctcgaagc
     2281 aggctcg
//
LOCUS       DASUOM010000151         5154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_267642,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000151 DASUOM010000000
VERSION     DASUOM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267642"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFY27_03000"
     CDS             complement(<1..316)
                     /locus_tag="VFY27_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764506.1"
                     /translation="MSNGGPPVRLARSLDRRGIAQVYSRASRVHIVPILAQDCAEHLL
                     RELSSSIPWQLHLNSGPRSINLEEDQFEALPAEDQQKILNSVHASAAEGFQFLYNRFP
                     VSD"
     gene            1116..1557
                     /locus_tag="VFY27_03005"
                     /pseudo
     CDS             1116..1557
                     /locus_tag="VFY27_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1744..2097)
                     /locus_tag="VFY27_03010"
     CDS             complement(1744..2097)
                     /locus_tag="VFY27_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764507.1"
                     /translation="MFRRCLAIAIFVALPGCVPFPVEPPISAAQNNLTGTDGLAGVTG
                     PIKTVNIRLGDQTRCENVTRTGSRIVVAQRCYSKQDEIDREERTAQQVEQARRDQDEL
                     DRRARDLEDQRRQRM"
     gene            2403..3293
                     /locus_tag="VFY27_03015"
     CDS             2403..3293
                     /locus_tag="VFY27_03015"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX5764508.1"
                     /translation="MKLQRGLAPPHRIRGTMSRLWLKAYPVGATDGWSIEPAPAKREW
                     MDATYKKVAYRCLPLIVANQAGWVVRCPANFTATWSGKIDNHGIKIDYAPDSAHRAGV
                     ALSHFGSGVLTFLLPWLFRTPPGIGLCIRGLPNDMRDSAVALDGIVETDWSPFTFTVN
                     WKLLRPDVPVHFKIGDAVCLIQPFALALLEQFDCSFEPYESAPIEIQQGLYDFVNTRT
                     VKNGVARQSEYETQRDYFAGRFPDGSLAHYPFGTATPEPIATCPHHDIPAAAGVVALA
                     PDAVPTPQHRTTFDLKPFSK"
     gene            3774..>5154
                     /locus_tag="VFY27_03020"
     CDS             3774..>5154
                     /locus_tag="VFY27_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5764509.1"
                     /translation="MAARPIAVVGEFGKPYVALLKVTPLYAGVCACLGLGSMAYGQQP
                     TETASPLEEVVVTGSRIVRRDLDAPSPILTVDSEAFDQNSSIGVEAVLNQYPQFNPGA
                     TQFNASQVTPNSAATPGVSTLNMRGLGDGRGLVLVDGRRVQPVNAGLAIDTNMIPSAM
                     IASVEVISGGAAATYGSDALAGVVNFKLRRDFEGIRINYQQGFMDAGDGEEQRADILV
                     GGSFDDNRGNAVLNVAWAHRSSASYASRDFYRKGYEDPGTDSNYPRISFPALDMSTGA
                     AGNYPSQAQLSQEFPNGVWVGGVLRPPGSIPNNLQFYINPDHNSVFLQEDAVRNGYTG
                     STEFPYKQRTQLNDTLQEVDTRRYLSSPLTRYSAFGRATYDIAQNISLFAQGTFVTSN
                     IWANGPPTPVSTPAIPRNPAMEPAVLRRLLDSRPTPGATYRLTEVAKYYDNRTTSNDT
                     HLFDFTAGLE"
BASE COUNT          998 a         1623 c         1547 g          986 t
ORIGIN      
        1 gatcggaaac cggaaagcgg ttgtagagaa actggaagcc ctctgccgcg ctggcatgca
       61 cgctgtttag gattttctgt tggtcttcgg cggggagcgc ttcgaactgg tcttcctcga
      121 ggttgatcga acggggtccg ctgttcaagt gcagctgcca cggaatgctc gagctgagct
      181 cgcgcagcag atgctccgca cagtcctgcg cgagaatcgg tacgatgtgc acgcgcgacg
      241 cacgcgagta gacctgcgcg atgccgcgac ggtcgagact gcgtgcaaga cgaacgggtg
      301 gaccgccgtt tgacatacaa agctgagcta cgaaatcgcg ggttcgtctc ggcagaaatg
      361 gcgaaagtcc atgctccacc gtagcaggag aaccggtccc tctccaaggg cgagtttctt
      421 gcgatttgcg acatcgggtc ggcgtcgagg acggtgtgcc tgcgattgcg cccgtgctgc
      481 gtgaggttga ccccgctcgc tggcccctcg ccacccgata cgtgatcgag tagcggcctc
      541 actcgcatgg gcgggagggt cgttcaggcc gtaacaccgc gtcgtagcag gccaagtcgt
      601 tcggcattcg ccacgatttc cgtgcagggc gtaccgggcg agtccctaag tagagtaagc
      661 aatgatcgtg tagcggcgaa gagcagtatc gctaggacgc caacctgctg cgccggctca
      721 tcgaggccga ggctccctcg ccgatcgacg ccccaggtgg gctgcgcttg tcgcattgcc
      781 tcacgaggaa tgttacccgc gagagtcgtt gcctcacccg aaatgttacc cctgatcggg
      841 agtgcaacac ggttccggga gggcgctggg gacgacacaa tgaacctcgc tgcgcgggcg
      901 gagctcgcca ccgctgttcg ccgccgtttc ggcgacgggc aaggagaagc tatgcgaagc
      961 gcctgagcgc cttactcccg atttgctttg ggacgacgtc ggtaacattg ctgctgaggc
     1021 agcgggtaac aaaattgggt gaggcaacac gacaacgctt ctcgaagagc ccgcagccaa
     1081 catcactcgc tacgaccacc tgcgaggagc gacccatggc cgagcagcac ggccccgcgt
     1141 tcaaacaggc cgagcgcatc ctctccgacc tcatcaaggc cgaagtcgcg gaacgggaag
     1201 tccgctcgat ccagtatcag ctgacgtccg tacgcttccc cgcgtatcgg gatctcgcgg
     1261 gcttcaagtt cgaggagagt gcggtggacg ttgcgcccgt gaagtcgctc catgactgcg
     1321 acttcatcga gcatgctcac aacgtggtgc tcatcggtgg ccccgcgcac cagtgtcgtg
     1381 atcacgacga acttgagctt ctcggaatgg gccaacgtgt tcggcgatcc gaagatgacc
     1441 accgcgctgc tcgatcgctt cacgcatcac tgccacatcg tcgagaccgg caacgaccta
     1501 ccgcttcaaa ctcacaaccg gagggaccga caactaaaga aacccgacga accatgagcc
     1561 ataattcggc tcactgaggc gggtcaactt tcgatgcaaa acccgggtca gctttcggcg
     1621 caaaacaaca acacgaggtc agaccgatgc tcaccgcatg agaacggcta tgctcgcctt
     1681 agccgatcgc ctacataccc gactgacgcg cagccaccta gcgcggacgc gaaacagctc
     1741 gcgctacatc cgctgccggc gctgatcttc gaggtcgcgg gcgcgccggt caagctcatc
     1801 ctggtcgcgc cgcgcttgtt ccacctgctg ggccgttctt tcttctcgat cgatctcgtc
     1861 ttgcttcgaa taacaacgtt gcgcgacgac tatccgcgat ccggtccggg tcacattctc
     1921 gcagcgcgtt tggtcaccca gcctgatgtt gacggtcttg atggggccag tgacgcccgc
     1981 gagcccgtcg gtaccggtga gattgttttg agcagcgctg atggggggct ccacggggaa
     2041 cgggacgcac cccggcagcg ctacgaaaat agctatagcc aagcaacgcc tgaacatcgc
     2101 aacgctcctt tcggaaaagc gtcgaattca cgtacacccc aaaataccgc catagcggcg
     2161 attctgccac cctaacccaa ctccagcagt tgggctggtg gttctggccg ttttcggtcc
     2221 tcggagctga gccggtctag cggtagcaag ctcccggaat ttccagcgga cactctctcc
     2281 tcgccatctt cgctgccgac tgggcgacgg ggttttgcct gctaccactt cggtggcctc
     2341 ggccacactt acggaaaaaa gcgtttgaaa tgactatccg cggaattact atcgctctgg
     2401 agttgaagct tcagcgcggc cttgctccgc cgcatcggat cagaggcacc atgtcgcgct
     2461 tgtggctcaa ggcgtacccc gtcggcgcga ccgacggttg gtccatcgaa cctgcgcccg
     2521 cgaagcgcga gtggatggac gcgacctaca agaaggtcgc ttaccgctgt ctgccgttga
     2581 tcgtcgccaa ccaagccggc tgggtcgtcc gctgccccgc gaacttcact gcgacttggt
     2641 cggggaaaat cgataaccac gggatcaaga tagactacgc tcctgactcc gcacaccgcg
     2701 ccggcgttgc gctgtcgcac ttcggcagtg gcgttctgac gttccttctg ccttggctgt
     2761 tccgcacgcc gcccggtatt gggctgtgca tccgcggctt gcccaatgac atgcgggaca
     2821 gcgccgttgc gctcgatggc atcgtggaaa ccgactggtc gccattcact ttcaccgtga
     2881 attggaaact gctgcgcccc gacgttcccg tccacttcaa gatcggcgat gcggtgtgct
     2941 tgatccagcc gttcgccctc gcgctgctcg agcagttcga ctgttccttc gagccgtacg
     3001 agtccgcgcc gattgagatt cagcaggggc tttacgactt cgtgaatacg cgcacggtga
     3061 aaaacggcgt cgcccgacag agtgaatacg agacgcagcg ggattatttt gcgggccgct
     3121 tcccggacgg ctcgctcgcg cactatccgt tcggcacggc taccccggag ccgatcgcga
     3181 cctgcccgca ccacgacatc ccggcggcag cgggcgtcgt cgcgcttgct cccgacgcag
     3241 ttccgacgcc gcagcatcgc acgaccttcg acttgaaacc gttcagcaag taaaccgaag
     3301 cggtggcgcc aacgaagaat cgcggcgctg tggcgttagc gcgcggctgc tggtcgggcc
     3361 cggcgccctc cggcagcgga tatcgatcga cgttgagcag catgcctttc ggcgtgagat
     3421 gcagcacacg gcacctcttg tctcggcgat cagggagatc agggacgctc gacatcagct
     3481 tgcagccctc cagcttgtcg agaggctgac gaagttgcca tgcttcttgt tgatggcgcc
     3541 cgtgagttcc gcttgctgca gagttgcatt ccatattcgg cgagactata agctccttaa
     3601 agaaattact gcatacatta gcctacacaa cctccgggtt cgccaggagt ttgttgcctc
     3661 aacgaaacac aataatggtg gtggaaaagc tctacaagtt tcgttggtga catggccgga
     3721 aaaaaattgc acattttttc atgttgcatc actgcgatac ccggggggga tttatggctg
     3781 ctcgacccat cgctgttgtt ggagagtttg gaaagcctta cgtagctctg ctgaaggtga
     3841 cgcccctcta tgcgggtgtg tgcgcctgcc tggggctcgg cagcatggct tacggccagc
     3901 agccgaccga gactgcgagt ccgctcgagg aagtcgtcgt cacgggttca cgcatcgtgc
     3961 gccgggacct cgacgctcca agtccgattc tgacggtcga ctcggaggcg ttcgatcaga
     4021 acagcagcat cggcgtcgaa gccgtcctca accagtaccc gcagttcaac ccgggtgcga
     4081 cacagttcaa cgcgagccag gtcacaccga attcggcggc tacgccgggc gtttcgacgc
     4141 tcaacatgcg cggtctcggc gatggccgag gtctcgtgct cgtggacggg cgtcgcgtgc
     4201 agccggtcaa cgcggggctt gcgatcgata ccaacatgat cccgtccgcg atgatcgcga
     4261 gcgtcgaggt catcagcggc ggcgccgccg cgacctacgg gtccgatgcg ctggctggcg
     4321 tcgtgaactt caagctgcga cgcgacttcg agggcatccg aatcaactac cagcaaggtt
     4381 tcatggacgc cggtgacggc gaggagcaaa gagccgacat tctggtcggc ggaagcttcg
     4441 acgacaaccg cggcaacgcg gtgctcaatg ttgcttgggc gcatcgctct tctgcctctt
     4501 acgctagccg agacttctat cggaaaggct acgaggaccc gggcacggac agcaactatc
     4561 cgcgcatcag cttcccggcg ctcgacatgt cgaccggcgc ggccggtaac tatccgtcgc
     4621 aagcgcagct gagccaggag ttcccgaacg gtgtttgggt gggaggtgtc cttcgcccgc
     4681 ccggctcgat ccccaacaat ctgcagttct acatcaatcc cgaccacaac agcgtgtttc
     4741 tccaagagga cgccgtccgg aacggctata cggggtccac agagttcccg tacaagcagc
     4801 gcacgcagct caacgacacg ctgcaagagg tggacacgag aagatatctg tcctcgccgc
     4861 tgacgcgcta ctcggcgttc ggcagggcta cctacgacat cgcccagaac atctcgttgt
     4921 ttgcgcaggg cacgttcgtg acctcgaaca tctgggcgaa cggacctccg actccggtga
     4981 gcacgccggc gatcccgcgc aacccggcga tggagcccgc ggttctgcgc cgtttgctcg
     5041 actcgcggcc gacgccaggc gctacctacc ggctcacgga ggttgctaag tactacgaca
     5101 accgcacgac gtcgaacgac acgcacctgt tcgacttcac ggccggtctc gagg
//
LOCUS       DASUOM010000152         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_143646,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000152 DASUOM010000000
VERSION     DASUOM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143646"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2220
                     /locus_tag="VFY27_03025"
     CDS             130..2220
                     /locus_tag="VFY27_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX5764510.1"
                     /translation="MSTRLAITVLSASILLGCGAPKTAPAPAPDANATASDVAASVET
                     AGSVQDDGNDVAFWIHPGDPAKSLVLVTAGTGGLEAFSLDGTKAAQYTAGEFDYVDVS
                     YGAGAGADPGAIVIAYDRKQRGLWALTIDPATLSIQPASKSAFRTGGEVTGLCSYRSP
                     VTGDHYAFAAANGLLEQWKLFVAGGEVRGTLVRSVPIGDGASYCAADPETESVYVTEE
                     SVGIWKLAAEPEFEANRTPMDLVAPFGALKEEVKGVSIYRVDSSTAYLLATDVADESY
                     TVYDLADHKLLGRFKITGDAKVADLDAVGESEGLTSTAFDFGGAYAGGLLAVFDESNE
                     GGRGNAKLVRWSAVAEALRLKSATGSEPRKVPSPKAVVVVPDVETVPVEDFGDAADDI
                     AIWAHPKDPNLGLVIGTNKKRGLDVYDLKGKRIQVLEDGRVNNVDLREGFRLDGRDVA
                     LVAVSNRTYKSLGLYRVDAQARRLVNVAEGVIEVGLGDPYGLCMYRSAKSGETYVFVN
                     DSDGRFKQFKLLATANKVRAELVREFALESQTEGCVADDANGALYLGEEDVGLWRYSA
                     EPDGGTARTSIDTTAEGGHLTADVEGMGLYLQAGGKGWLVVSSQGSNDYAVYRREGAN
                     EYVGRFSVVANDASGIDGISETDGLDVSSANLGGIYAQGIFIAQDGRNITPAERQNFK
                     FVAWEKIARAMKLQ"
     gene            complement(2225..>3530)
                     /locus_tag="VFY27_03030"
     CDS             complement(2225..>3530)
                     /locus_tag="VFY27_03030"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5764511.1"
                     /translation="FGYPRLLQGPMLGASGPTSLIIWGRASGALPVSVQYSTSREMLD
                     ARVTPTVHASIESDFTAVIRIADLKPDTTYWYRVLVDGEVDRYRTQPSSAKTAPSQSQ
                     NFRIAFGSCARLARDPEQRIFRIVDAQSPDLFFWLGDNVYADAESTFAFADEYRRQRN
                     VPSLQNVIRRIPQLAIWDDHDFGYNNSDGSFPFKDQSLTAFKQYWPNPAYGEPGNPGV
                     YFEYSYAGIDFFFLDGRYHRTPNAAPDGPSKKFLGDLQTDWLKQRLLASRAPFKILAC
                     GSGWSMADGPQGDTWAAFKTARDDLFDFIRDRRIGGVVCLSGDSHVGELNCIPCSERG
                     GYDIYDLVSSPLAQSCDMSWVTQSPEVRMRPVYAGGANVGMLDFVSGANPTLTFSLFN
                     EWGDMAWKPLTLTPADLRNGVSSWRTKIDEKELAKREKRSAS"
BASE COUNT          669 a         1077 c         1153 g          631 t
ORIGIN      
        1 ggagagcatt catgcgtgtg ccgtatcccg tgaaattggc agactgcgtg caccgcacgg
       61 ctgctgcttg aaattcgtgt tccaacacta taaaactatg gaatgaaaat tccaaacgga
      121 aactcatgta tgtcgacccg tctcgcgatt accgtccttt cggcctcgat attgctgggc
      181 tgcggcgcgc caaagaccgc gccggcacct gcgcccgatg cgaacgcgac tgcctctgac
      241 gtcgccgcgt cggtcgaaac agcgggcagc gtccaggatg atggcaacga cgtcgcgttt
      301 tggatccatc ccggtgatcc tgcaaagagc ctcgtgctgg tgaccgcggg caccggcggt
      361 cttgaagcgt tctccctgga cggcacgaag gccgcgcagt acacagccgg cgaattcgac
      421 tatgtcgatg tgagctatgg cgcgggcgcg ggcgccgacc cgggcgccat tgtcattgcc
      481 tacgatcgca agcagcgagg tttatgggcg ctcaccatcg accccgcgac tctgtccatt
      541 cagccggcca gcaaatcagc gttccgcacg ggcggcgagg tcacgggcct ctgcagctac
      601 cgcagccccg tgaccggcga tcactacgca ttcgcggcgg cgaacggact cctcgagcag
      661 tggaagctct tcgttgcggg aggcgaggtg cgcggcactc tcgtgcgctc ggttcccatc
      721 ggtgacggcg cgagctactg cgcggcggac ccggagaccg agtccgtcta cgtcaccgag
      781 gaatccgtcg gcatctggaa gctggccgcg gagcccgaat tcgaggcgaa tcgtacgccc
      841 atggatctcg tcgcgccttt cggcgcgctc aaggaagagg tcaagggcgt ctcgatctac
      901 cgcgtggatt cctcgaccgc ttatctgctc gcgaccgatg tcgcggacga aagctacacc
      961 gtctatgacc tcgccgatca caagcttctg ggtcgcttca agatcacggg cgacgcgaag
     1021 gtcgccgatc tcgatgccgt cggggaatcc gaggggttga cttcgaccgc cttcgacttt
     1081 ggcggtgcgt acgccggcgg attgctggcg gtgttcgacg agtcgaacga agggggacgt
     1141 ggcaacgcca agctcgtccg ctggagcgcg gtggccgaag cgctgagact caaatctgcg
     1201 actggcagtg agccccgcaa ggtgccctcg ccgaaggccg tcgtcgtcgt gcccgacgtc
     1261 gaaactgtgc ccgtggaaga ttttggcgac gcggcagacg acattgcgat ctgggcgcat
     1321 ccgaaggatc ccaacctggg gctcgtcatc ggaacgaaca agaagcgggg cctcgacgtg
     1381 tacgacctga agggcaaacg catccaggtg ctggaagacg gccgcgtcaa caacgtggat
     1441 ttgcgcgaag gcttccggct cgacgggcgt gatgttgcgc tggtggccgt ttccaaccgc
     1501 acttacaaga gcctgggcct ctatcgtgtc gatgcgcagg cgcgacgcct ggtcaacgtc
     1561 gcggaaggag tcatcgaagt cgggctcgga gacccctatg ggctgtgcat gtatcgcagc
     1621 gcgaaatccg gggagacgta cgtcttcgtg aacgactcgg atggcaggtt caagcagttc
     1681 aaactcctcg ccacggcgaa caaggtccgg gcggagttgg tgcgcgagtt cgcgctcgag
     1741 tcccagacgg agggatgcgt ggccgatgac gcgaacggtg cgttgtacct gggcgaggaa
     1801 gacgtcggcc tctggcgcta ctccgcggag ccggatggag gcacggcacg cacgtcgatc
     1861 gataccactg cggaaggcgg ccacctcacc gcggacgtgg aaggaatggg cctctacctg
     1921 caggcgggtg gcaaaggctg gcttgtcgtt tcgagccagg gatcgaacga ctacgccgtg
     1981 tatcgccgcg aaggagccaa cgaatacgtt ggtcgcttct cggtcgtcgc caatgatgcg
     2041 tccggtatcg acggcatctc ggaaaccgac ggcctcgacg tgtccagcgc caatctcggc
     2101 gggatctacg cgcagggtat cttcatcgcg caggatggcc gcaacatcac gcccgccgag
     2161 cggcagaact tcaagttcgt ggcttgggag aagattgccc gcgcgatgaa actgcagtga
     2221 gcggttagct cgcggagcgc ttctcccgct tcgcgagctc cttctcgtca atcttggtgc
     2281 gccagctgct gaccccgttc cggaggtcgg cgggcgtgag ggtgagtggc ttccaggcca
     2341 tgtcgcccca ttcattgaag agcgagaacg tcagcgtggg gttcgcaccc gagacgaagt
     2401 ccagcatgcc cacgttcgcg cctccggcat acacaggcct catgcggacc tccggcgact
     2461 gcgtcaccca ggacatgtcg catgactgcg cgaggggcga gctgaccagg tcgtagatgt
     2521 catagccgcc gcgctccgag cacggaatgc aattgagttc gcccacatgg ctgtcgcccg
     2581 acaggcagac cacgccgccg atgcgccggt cccgaatgaa atcgaacaga tcgtcacgag
     2641 ccgtcttgaa cgcagcccag gtgtcgccct gcggtccgtc ggccatcgac cagccgctgc
     2701 cgcacgcgag tatcttgaac ggggcgcgac tcgcgagcag tcgctgcttc agccagtccg
     2761 tctgcaggtc gccgagaaac tttttcgacg gcccatccgg agccgcattc ggggtcctgt
     2821 ggtagcgacc gtcgaggaag aagaagtcga tgccagcgta agagtattcg aaatagacgc
     2881 cgggattgcc gggctcgccg tatgccggat tcggccagta ctgcttgaaa gcggtgagtg
     2941 actgatcctt gaacgggaag ctgccgtcac tgttgttgta gccaaagtcg tggtcgtccc
     3001 agatcgccag ctgcggtatg cgccggatca cgttctgcag cgacgggaca ttgcgttgcc
     3061 ggcggtattc atccgcgaag gcgaaagtgg actccgcgtc cgcgtagaca ttgtcaccca
     3121 gccagaagaa caggtccgga gactgggcgt cgacgatacg gaagatccgc tgttccgggt
     3181 cacgcgccag gcgagcgcac gatccgaacg cgatgcggaa attctgcgac tgggacggcg
     3241 ctgtcttcgc ggaggagggc tgggttcgat agcggtccac ttcgccatcc acgagcaccc
     3301 ggtaccaata cgtcgtatcc ggcttgaggt ccgcgatgcg gatgaccgcc gtgaagtcgc
     3361 tctcaatgct ggcatggacc gtgggcgtca ccctggcatc cagcatctcg cgcgaagtcg
     3421 agtattgcac cgagacgggc aaggctcccg aagcccgtcc ccagataatc agagatgtcg
     3481 ggccgctggc gcccagcatc ggtccctgca gtaacctggg atatccaaag
//
LOCUS       DASUOM010000153         6220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1217365,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000153 DASUOM010000000
VERSION     DASUOM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217365"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..1704
                     /locus_tag="VFY27_03035"
     CDS             250..1704
                     /locus_tag="VFY27_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5764512.1"
                     /translation="MLSVNKNSDDFLYRQVIDLISENIDSGTLRPGDRLPSLRRMSTR
                     IGVSIPTVRQAYVELERQRRVESRPQSGFYVRGFLSNDMVRPAPRFRRTAKPVPVSRR
                     SLMDRVFDGIHQPDVLPFGIADPSMAKPPAKALHRTMKRVMARAEERSLAYAPNNGEP
                     GLRRQIAYRYLDSVGSNVDPDGICITNGGQEALSLALAAVAKRGDVIAVESPTYHGQL
                     ELIESMGMLAIEVETCPEEGVELDALRKTLDAHPVKVCMFSTTLSNPLGASMPDGKRK
                     ELVELLDEQEIVLIEDDVYGELLFDGQRPKPAEFHSHSGRILTCGSFSKTAAPGYRIG
                     WLLTGRHHDEVVRLKRSFSCSSGLLQQMTLAEFVASGDYDRHLKTLRQVLQCNARRMT
                     AAIASYFPPETRISKPVGGCVMWLELPEAVDSERLFSLAIRDGISIAPGSIFSPCNRY
                     RNFIRLSFGHPWTAEIEAGLKKLGDMVIRLAGTA"
     gene            1795..3039
                     /locus_tag="VFY27_03040"
     CDS             1795..3039
                     /locus_tag="VFY27_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5764513.1"
                     /translation="MIRAIASITALLVGVAILLTGQGLQGTLLPVRAAMEDFSTMAIG
                     AFGAAYFLGFTVGSWRGAGLVRRVGHVRVFAAMTALASASPLLHGMWIDMLSWCVLRF
                     LSGFCFAILYVVIESWLNETSTNENRGSVFSIYVLINMTMLAAGQQMMMLDDPAVMTL
                     FALASVLVSLAAVPVSLSTAPTPRQLHGDTRLDIRHLYRTSPAGMLGSLATGLANGSF
                     WALAPVFTISYSTDVSLTAWFMTAAVLGGAASQWPLGAWSDRIDRRFVMAIAASLAIG
                     VAFAIWLFADRFTHGGVILLGGLWGAAAFPLYSISVAHSNDHAAEHEYVMVSSGLLLM
                     YGIGAVVGPVVSSIAMNRLGSGGLFAFTGAMHMLLFVYLLQRRLRRRTVDAEDHVAFA
                     DALKASQTTSQVYETEMASKVK"
     gene            3244..5091
                     /locus_tag="VFY27_03045"
     CDS             3244..5091
                     /locus_tag="VFY27_03045"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX5764514.1"
                     /translation="MNTETMHGRVSSAAAPLLLAFALLWSGSLLAQVPVDEDGNPLEA
                     VDLEATGGEPLGESTAGETDATTGDEVPPMTPEELEALVGPIALYPDKLLAIVLPAST
                     YPLEIVQAARFLEQLEDDSSLKPDETWDDSVTALLNYPEVVKMMNDDIDWTWRLGEAV
                     VRQQPDVIAAVESFRDKAYAAGNLKTDERQRVSNNDGVIEIDPIEEDIIYVPYYEPER
                     VVYYQPRQVYYYYPRPYPVYYYPYPEHHAFYAGYFWGVTTAFRIGWATDHLHVFHHSY
                     WGHPYYGRQYFGHWYRRPSITVYNRFYPNSHRYRSDDHHRNGDYWRPRDGGARPGLYR
                     ARLERFGESSRASSIEGYRVPDRNGRSIASDRNNNSAGFRGDDGNSIRSGETREGFAA
                     SRRAQTREREDLVRTARATRPAAGASNDTIRFRPRTRASADGVADRVQPERRQESDTP
                     VRTPERAARPPQQSDGIDTIRFRSRSNPDGAASARPSIPASPSTLNSAARARAGTNDR
                     PVVRDRSNLEASLPEARRAPSTSGFRASPGRSAPAARPDVSRPQASPRAAPAPKVSMP
                     RATPRAAPAPRASSTPAPRASAPARAPEASSSPRRSSGTSTRTQRTREH"
     gene            complement(5097..5270)
                     /locus_tag="VFY27_03050"
     CDS             complement(5097..5270)
                     /locus_tag="VFY27_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764515.1"
                     /translation="MNLLSLIIIFLAIAAAGGIIYAAWELSSDKPRQGKAGSNDVGSS
                     GPAGSSGSDQPPP"
     gene            5402..>6220
                     /locus_tag="VFY27_03055"
     CDS             5402..>6220
                     /locus_tag="VFY27_03055"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5764516.1"
                     /translation="MSAGNKSARSISFLSAVLCATLLAACGPSEDTTTPAAQPGAQRE
                     AGGPAPIEASAKARELARSSIIVDTHIDVPYRLDEEWADVSEATDGGDFDYPRAVAGG
                     LNAPFMSIYTPAELQKEGGARELAEKLIDMVNEIAAQSPDKFAVAYSPLDIERQFSEG
                     LISLPMGMENGAPIEDDIANVSHFYDRGIRYVTLAHSLSNLISDSSYDDNKQWGGLSE
                     FGMDVVREMNRLGIMVDVSHISDEAFWDVMEVTSAPVIASHSSARHFTPGWERNI"
BASE COUNT         1149 a         1976 c         1937 g         1158 t
ORIGIN      
        1 aggatccgcg tgcggatgta gcggtacctg tgcagttcga gcgccgaccg ggcgaggtag
       61 ccgagggcgc cgccaagcga caggcccagg cgcccggttg cggtccggtg gcaagtgctt
      121 cggcatgttg cggtattcat agcagtctcc gttccttgcg gggcctactt ggctccgcgg
      181 aggttgacta tacggcccta ttggagctat aacagattca gttttttgaa aatgtgacat
      241 gaacagatta tgctcagcgt caacaagaac agcgacgact tcctgtaccg gcaggtcatc
      301 gacctgatct ccgagaacat cgactcgggg accctgcgcc ccggagaccg gctcccctcc
      361 ctccgccgaa tgagtacccg cattggcgtc agcattccta cggtgcggca ggcctatgtc
      421 gaactggagc gccagcggcg cgtcgagtcg cgtccgcagt cggggtttta cgtacgggga
      481 tttctctcca acgacatggt gcggccggcg ccgcgctttc gccgcacggc gaagcccgtg
      541 ccggtttcgc gtcgcagcct gatggaccga gtcttcgatg gcattcacca acccgacgtc
      601 ctgccgttcg gcatcgcaga ccccagcatg gcgaagccgc cggccaaagc cctgcaccgg
      661 acaatgaagc gcgtgatggc gcgcgcggaa gaacgcagcc ttgcctacgc gcctaacaac
      721 ggtgagccgg gccttcgccg gcagatcgcg tatcgctacc tcgactcagt cggcagcaac
      781 gtcgatcccg acggcatctg cattacgaac ggcggccagg aagccctgtc tctcgctctt
      841 gccgccgtcg cgaagcgcgg cgacgtcatt gccgtggagt cgccgactta tcatggccaa
      901 ctcgagctga tcgagagcat gggcatgctg gcgatcgaag tcgagacctg cccggaagaa
      961 ggcgtcgagc tggatgcgct gcgaaagaca ctggatgctc acccggtcaa ggtgtgcatg
     1021 ttttctacca ctctcagcaa tcccctgggc gccagcatgc cggacgggaa aaggaaggag
     1081 ctggtcgaac tgctggacga gcaggagatc gtgctgatcg aggacgatgt ttacggcgag
     1141 ctgctgttcg acgggcagag gccgaaacca gcggagttcc actcgcatag cggccgcatt
     1201 ctgacctgcg gttcgttctc taagacggca gctccggggt accggatcgg ctggttgctg
     1261 acaggccggc atcacgatga ggttgtgcgg ctcaagaggt cgttttcctg ctcgtcgggg
     1321 ctgctgcagc agatgacgct tgcggaattc gtcgcaagcg gcgattacga caggcacctg
     1381 aagacgcttc gccaggtcct gcagtgcaac gcacggcgca tgacagccgc gatcgcgagt
     1441 tacttcccgc cggagacccg catttccaaa ccggtgggtg gctgcgtcat gtggctcgaa
     1501 cttccggaag cagtcgattc ggaaagactg ttcagcctcg caatcaggga cggcatcagc
     1561 atcgctccgg ggagcatatt ctcgccctgt aatcgctacc ggaatttcat aaggcttagc
     1621 ttcggccatc cctggaccgc ggaaatcgag gcaggtctca aaaagctcgg cgacatggtg
     1681 ataaggctcg cgggaactgc ctgacttttc tttcaggaac tgcgagcatt cctctttacc
     1741 cgcgggtcgc ttcccctgaa actcgcgtca ccttcttaag ctccagtttt cttcatgatt
     1801 cgagcgatcg cctctatcac tgcgctgctg gtcggcgtcg caatcctgct cacgggccag
     1861 gggctgcaag gtacgctgct gccggtgcgc gcggccatgg aggatttctc gaccatggcc
     1921 atcggcgcgt tcggcgccgc ctacttcctc gggttcactg tcggctcctg gcgaggcgcc
     1981 ggcctcgtcc ggcgggtagg acacgtccgc gtgttcgccg cgatgaccgc gctggcttcg
     2041 gcaagcccgt tgctgcacgg catgtggatc gacatgttgt cctggtgcgt gctgcgcttc
     2101 ctgtccggct tctgcttcgc catcctgtac gtggttatcg agagctggct caacgaaacc
     2161 tccacaaacg aaaaccgcgg ctccgtcttc tcgatttacg tgctcataaa catgacgatg
     2221 ctggcggcgg ggcagcagat gatgatgctc gacgatccgg cagtgatgac gctgttcgcg
     2281 ctggcgtccg tgctcgtctc gctcgcggcg gtgcctgttt cgctgtcgac ggcccctacg
     2341 ccgcgccaac tgcacggaga cacgaggctc gatatccgcc acctgtaccg cacgtcgccc
     2401 gccggcatgc tcggcagcct cgcgacggga ctggcgaacg gctcgttctg ggctctcgca
     2461 ccggtgttca cgatctccta ctccaccgac gtatcgctga ctgcctggtt catgaccgcg
     2521 gctgttctcg gtggcgctgc cagccagtgg ccgttaggcg cgtggtcgga ccgcatcgac
     2581 cgccgcttcg tgatggcgat cgccgcgagc ctcgcaatcg gggtcgcgtt tgccatttgg
     2641 cttttcgccg accggtttac gcacggcgga gtgattctgc ttggtggatt gtggggtgcg
     2701 gcagctttcc ccctctactc gatctcggtt gcccattcga atgaccacgc cgccgagcac
     2761 gaatacgtga tggtgtcgag cggcctgctg ctcatgtacg ggatcggcgc ggtcgtgggg
     2821 ccggtggtct cgtccattgc catgaaccga ctgggctcgg gcggcctgtt cgcgttcacc
     2881 ggcgccatgc acatgctgct gtttgtctac ctgctgcaac ggcgcctccg gcggcggacg
     2941 gttgacgcgg aggaccacgt agccttcgcg gatgcgctga aggcctccca gaccacctcg
     3001 caggtgtatg agacggagat ggcgtcgaaa gtaaaatagt gttgtctaat caatagcttt
     3061 tcaggctttc atcgatttac tgtaaagatc cctggcacat gcccggccgt ccgggcgttg
     3121 agccgggatt ccagttaagc gcctgttcag cttgaccctt ctaccatcgc ccccaagatg
     3181 tctcccgtcc gcctgcaaga atgcaagcgc gtaaaggggg ataatggatc cggaggtccg
     3241 cacatgaata cggaaacgat gcacggaaga gtgtcgagcg ccgcagcccc gctgctgctc
     3301 gcctttgcat tgctatggtc cggttcatta ctggcgcagg tgcccgtcga tgaagacggc
     3361 aatccgctgg aggcggtcga tctcgaggca accggcggag aaccgctcgg cgaatccacc
     3421 gcgggagaaa ccgacgccac cacaggggat gaggtgccgc cgatgacgcc ggaagagctc
     3481 gaagcgctgg tggggccgat tgccttgtac cccgacaagt tgctcgcgat cgttttgccg
     3541 gcttcgacct atcccctgga gatcgtgcag gcagcgcgtt tcctcgagca actcgaagac
     3601 gacagcagcc tgaagcccga cgagacgtgg gacgattcgg tcactgcgct cctgaactac
     3661 cccgaagtcg tcaagatgat gaatgacgac atcgactgga cgtggcgcct cggggaagcc
     3721 gtcgtgcgcc agcagccgga tgtcatcgcc gccgtcgagt ctttccgcga caaggcctat
     3781 gccgccggca acctgaagac cgacgagcgc cagcgggtga gcaacaacga tggcgtcatc
     3841 gagatcgacc cgatcgaaga ggacatcatc tacgttccgt actacgagcc ggagcgcgtg
     3901 gtgtactacc agccgcggca ggtttactac tactacccgc gtccctaccc ggtctactac
     3961 tatccatacc ccgagcatca cgccttctac gcaggctact tctggggcgt gaccacggca
     4021 ttccggatcg gctgggccac ggaccacctg cacgtgtttc atcacagcta ctggggccac
     4081 ccgtattacg gccgccagta cttcggtcac tggtaccgcc gtccgagcat caccgtctac
     4141 aaccggtttt atccgaacag ccaccggtac cgttcggacg atcatcacag gaacggggat
     4201 tactggcggc cgcgcgacgg cggtgcaagg ccgggccttt accgggcgcg cctcgagcgc
     4261 ttcggcgaga gctcccgggc gagttcgatc gagggttatc gcgtgcccga tcgcaacggc
     4321 cggagcatcg caagcgacag gaataacaat agcgcagggt tccgcggcga cgacggcaat
     4381 agcattcgca gtggtgagac gagagaaggt tttgcggcaa gccgtcgcgc gcagacgcgc
     4441 gagcgcgagg acctcgtccg tacagcgcgt gcgacgcgtc cggccgccgg agccagcaat
     4501 gatacgatcc gcttccgccc gcgcacgcgg gcaagcgccg acggtgttgc cgacagggtg
     4561 cagccggagc gccggcagga atcggatacg ccggtgcgca cgccggaacg cgcagcacgc
     4621 ccgccccagc aaagcgacgg catcgatacc atccgtttcc gctcgcgcag caacccggac
     4681 ggggctgcga gcgctcgccc gagcatcccg gccagcccat cgacattgaa cagcgccgca
     4741 cgcgctcgcg ccggcactaa cgatcggcct gtggttcgcg atcgctcaaa cctggaagcc
     4801 agcctgccgg aagcaagacg ggctccgtct accagcggct ttcgcgcaag ccccggccgt
     4861 agcgcacccg ctgcgcgacc ggacgtgagc aggccgcaag cgagcccgcg ggctgctccg
     4921 gcacccaagg taagcatgcc ccgggcaact ccgcgtgcgg caccggcacc gagagcaagt
     4981 tcgaccccag ctccgcgggc aagcgctccc gcgcgggcac cggaagcttc ttcctccccg
     5041 cgccgcagtt ccggcacgtc tacgcgtact cagcgtaccc gcgaacactg acatttctag
     5101 ggagggggct ggtcgctgcc tgagcttccg gcgggaccgg aactgccgac atcgttgctg
     5161 ccggctttcc cctgccgcgg cttgtccgaa gagagctccc aagccgcgta gatgatgccg
     5221 ccggcggctg cgatggccag gaaaataatg atgagcgaga gcaggttcat cgtgcagcga
     5281 tcatagctgc ttgtcccgcc atttccacgc gtaatgtggc acgggagccc aagcaagcgc
     5341 cactttccct atactgtgcg actcattgcg acgggcaacg tcacaaacat ggggtaagaa
     5401 aatgtccgca gggaataagt ccgcacgttc catctcgttt ttatccgccg tcctctgcgc
     5461 cacgctgctt gcagcctgcg gcccttcgga agacacgacc acaccggcag cgcaacccgg
     5521 cgcgcagcgg gaagccggcg gaccggcacc gatcgaagca agtgcgaaag cccgggagct
     5581 tgcaaggagt tcgatcatcg tggatacgca cattgacgtg ccgtatcgcc tggacgagga
     5641 atgggcggac gtttccgagg ccactgacgg aggcgacttc gactacccgc gtgccgtggc
     5701 gggcggcctg aatgcgcctt tcatgtcgat ttacacgccg gcggagctgc agaaagaagg
     5761 tggtgccagg gagctggcgg aaaagctgat cgacatggtc aacgagatcg ctgcgcaatc
     5821 gcccgacaag ttcgcagtcg cttattcgcc gctggatatc gagaggcagt tcagcgaagg
     5881 cctgatttcc ctgccgatgg ggatggagaa cggagccccg atcgaagacg acatcgccaa
     5941 cgtttcccac ttttatgacc gcggtattcg ctacgtgacc cttgcgcact ccctgtcgaa
     6001 cctcatttcc gattcttcct atgacgacaa caagcagtgg ggaggcttga gcgagttcgg
     6061 catggacgtg gttcgggaga tgaaccggct ggggatcatg gtcgacgtca gccacatttc
     6121 cgacgaggcg ttctgggacg tcatggaagt taccagcgcc ccggtcatcg cctcgcactc
     6181 ttccgcgcgc catttcaccc ctggctggga acgcaacata
//
LOCUS       DASUOM010000154         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1014236,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000154 DASUOM010000000
VERSION     DASUOM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014236"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFY27_03060"
     CDS             <1..317
                     /locus_tag="VFY27_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764517.1"
                     /translation="HYLMFQTMSFPDAPAPPVPEDFSLSQRERIDAPGWPKLGFIERS
                     LAGDLTNWWPANDACAEAMLRSSGFKVLERPEHETYWCRSAEGEDSVRDELESVMRGR
                     ET"
     gene            314..1162
                     /locus_tag="VFY27_03065"
     CDS             314..1162
                     /locus_tag="VFY27_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5764518.1"
                     /translation="MTTPICNTFGATGLKVPVVGQGTWNMERNPKMSVDALRYGIELG
                     LTHIDTAEIYGDGAVETLVGHALHGLRERVVLVSKVHPERASRDGIVRACEQSLQRLG
                     TSYLDVYLLHWLSPHPPAETIAGFEALVNAGKIRHWGVSNFDEVKLREFVDIAGATAI
                     CCNQVMHHLGERSIEHAVVPFCHDQRIAVVGYSPFGKHFPPPSRSGSDVLADVALTLD
                     ATPRQVALAFLVRKSGGFTIPKASNRQHVEENARAAALDLPDEAVERLDEEYPLGPRA
                     EGVPVW"
     gene            1254..2093
                     /locus_tag="VFY27_03070"
     CDS             1254..2093
                     /locus_tag="VFY27_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764519.1"
                     /translation="MNRHEYRVIGMSRSGNHAIIDWILSQASGRTCFLNCAEPQSNPF
                     STARQLDYGCRIVANYPEFDVEAELRGDFTDKHLLLVSHEDCFLGTVARGSYEDNHDA
                     LVGPSLERRDILILRDPFNLFASRMRGEFGHVSTTTALRIWKQHAREYLGARRYLKHP
                     RILIDYNQWASRKSYRRQIAKQLELHFTDASFEKVPATGNGSSFDGRRYNGRATCMRI
                     LERWKHFIDSEAYAALFDHEVYTLSQRIFGDIGYPGPRPERPGRKAAGSVALDATAAL
                     LPD"
     gene            complement(2176..>2844)
                     /locus_tag="VFY27_03075"
     CDS             complement(2176..>2844)
                     /locus_tag="VFY27_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5764520.1"
                     /translation="VVSIRDNGIGMPADRLDTIFEMFSQVPASSPQSTVGLGIGLTLV
                     KRLVELHGGSIRAWSEGPGKGSEFVVRLPVSITEQLSEESIEPIHDKACPPRRILVVD
                     DNRDSADSLALLLNLQGHEVEAVYDGLSAVHTAERFAPDVVLLDRGLPILDGIEACRR
                     IREKSWGRNMVIVALTGWGQEEDRRRSREAGFDEHLVKPVSNDALRKLLSQFLPLPNP
                     RHDV"
BASE COUNT          575 a          884 c          853 g          532 t
ORIGIN      
        1 cgcactacct gatgttccag acgatgtcgt tccccgatgc cccggcgcct ccggtgccgg
       61 aagatttcag cctcagccaa agggagcgta tcgacgcccc gggctggccg aagctcgggt
      121 tcatcgaacg gagcctggcc ggggacctga ccaactggtg gccggcgaac gacgcctgcg
      181 ccgaagcgat gcttcggagc agcgggttca aggtgctgga acgcccggag cacgaaacct
      241 actggtgcag gagtgccgag ggggaggatt ccgtgcggga cgaactcgag agcgtcatgc
      301 gcgggcgcga aacatgacga cgccgatctg caacaccttt ggcgcgaccg gactgaaggt
      361 gcccgtagta ggacagggaa cgtggaacat ggagcgcaac ccgaagatga gcgtcgatgc
      421 actgcgttac ggcatcgaac tcggactgac gcacatcgat acggctgaaa tttacggtga
      481 cggtgcagtc gaaactttag tcggccacgc gctgcacgga ctgcgggaac gggtagttct
      541 cgtatccaag gtgcaccccg aaagggcttc acgcgatggc atcgtccggg cctgcgagca
      601 gtcattgcag cgcctgggca ccagttacct cgacgtctac ctgctgcact ggctgtcgcc
      661 tcacccgccg gcggaaacaa tcgccggttt cgaggcactg gtgaacgctg gcaaaatacg
      721 tcactggggc gtaagcaact tcgatgaggt gaagctccgg gagttcgtgg acatagccgg
      781 agcgaccgcg atctgctgca accaggtgat gcatcacctc ggcgaacgct ccatcgagca
      841 tgcggtggtg cccttctgtc acgaccagcg catcgcggtc gtcggctaca gcccgtttgg
      901 caagcatttc cctcctccat cgcgctcagg gtcggacgta cttgcagacg tggccttaac
      961 cctcgatgcg accccgcgac aggtggcact cgctttcctc gtgcgaaagt ccggcggttt
     1021 cactataccc aaagcaagca atcggcagca cgtggaggag aacgcacgtg ctgctgcgct
     1081 cgatctgccg gatgaggcgg tggagagact ggatgaagaa tatcctctcg ggccgcgagc
     1141 cgaaggtgtg ccagtatggt gaggtcaccg gcaccttcga tttaacagcc tccgctcctt
     1201 attcgcccga tataatctcc gcctctcaag gcaaaggagc ttacatcggg caaatgaaca
     1261 ggcacgagta ccgcgtcatc gggatgtcgc gcagcggcaa tcacgccatc atcgactgga
     1321 tcctgtcaca ggcttccggc cgcacctgct tcttgaactg tgcggaaccg caatccaacc
     1381 cgttcagtac cgcccgccag ctcgattacg gctgccgcat tgttgccaac taccctgagt
     1441 tcgatgtcga agcggagctc cgcggagatt tcactgacaa gcatctgctg ctcgtcagtc
     1501 acgaggactg tttcctgggt accgtcgcca ggggttcata tgaagataac cacgacgcct
     1561 tggtcggccc ctctctcgag cgacgcgata tattgatact gcgcgatccc ttcaacctct
     1621 tcgcgagccg catgcgcggg gaattcggcc acgtgtccac aaccacggca ttgagaatat
     1681 ggaagcagca tgcgcgcgag tacttgggcg caagacgtta cctcaaacac ccccgcatcc
     1741 tgatcgacta caaccagtgg gcgtcacgaa aatcctaccg ccggcaaatt gcgaagcagc
     1801 tggaattgca cttcaccgat gcgagcttcg agaaagtgcc ggcaacaggc aatggcagtt
     1861 cattcgacgg ccgccgctac aatggacgcg ccacctgcat gcgcatcctc gagcggtgga
     1921 agcacttcat cgacagcgag gcttatgcag cgctgttcga ccacgaggtt tataccctgt
     1981 cgcagcgcat attcggcgac atcggatatc ccggcccgcg gccggagcgc ccggggagga
     2041 aagcggcagg ttccgtggca ctggatgcga cggcggcact gttaccggat tagtgagcgc
     2101 ccgcaggaga gctgtcgtgc gggagattcc atcaacgtct tcacactcgc ccgcatggcg
     2161 ggctcctaca gcgcgctaaa cgtcgtgccg cgggtttggt aacggcagga actgactcag
     2221 taacttccgg agagcatcgt tgctgaccgg tttcaccagg tgctcatcga agcctgcctc
     2281 tctcgacctg cggcgatctt cttcctggcc ccagccggtc agagcgacga taaccatgtt
     2341 cctgccccag gacttttcgc ggatgcggcg gcaagcttcg atgccgtcca gtatcggcaa
     2401 gccccggtcg agcagtacta cgtcaggcgc gaaacgctcg gcggtgtgca cggccgagag
     2461 gccgtcgtag actgcttcca cttcatgacc ttgcaggttc aggagcagtg ccagcgagtc
     2521 ggcactgtcg cggttgtcgt ccacaaccag gatgcggcgc ggcgggcacg ccttgtcgtg
     2581 gatcggttct atggattcct cgctcaactg ctctgtgatg gaaaccggca ggcgcacgac
     2641 gaactcgctg cccttacctg gcccttcgct ccacgcccgg atactgccgc cgtgcagctc
     2701 gaccagccgt ttcaccaggg tgagccctat cccaagacct acagttgact gcggcgagga
     2761 agccggtacc tgggaaaaca tctcgaagat ggtatccagc ctgtcggccg gcattcctat
     2821 gccgttgtcc ctgatcgaaa ccac
//
LOCUS       DASUOM010000155         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1473358,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000155 DASUOM010000000
VERSION     DASUOM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473358"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="ruvB"
                     /locus_tag="VFY27_03080"
     CDS             complement(<1..599)
                     /gene="ruvB"
                     /locus_tag="VFY27_03080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5764521.1"
                     /translation="MTAIERDRLASAAPRPEDRVFDRAIRPRSLADYVGQPAVRQQME
                     IFITAARNRREALDHVLIFGPPGLGKTTLAHIIAYEMGVNLRQTSGPVLERPGDLAAI
                     LTNLEPNDVLFVDEIHRLSPVVEEVLYPALEDFQLDIVIGEGPAARSIKLDLAPFTLV
                     GATTRAGLLTSPLRDRFGIVQRLEFYSTAELEAIAHRSAG"
     gene            complement(603..1193)
                     /gene="ruvA"
                     /locus_tag="VFY27_03085"
     CDS             complement(603..1193)
                     /gene="ruvA"
                     /locus_tag="VFY27_03085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5764522.1"
                     /translation="MIGSVRGRLTLKQPPRILVECAGVGYEIETPMSTFLELPQVGSD
                     IFLHTHLQVREDAQSLFGFATEEEKHLFRSLLRVSGVGAKMALAILSGMSVADFHRCV
                     LHEDSAMLVKIPGVGRKTAERLIIELRDRINQQATMPKSAAGRVMRADARSEAFDALV
                     ALGYKPQEVKRLMGQLDLNDKSAEDIIRSALRQAVG"
     gene            complement(1190..1711)
                     /gene="ruvC"
                     /locus_tag="VFY27_03090"
     CDS             complement(1190..1711)
                     /gene="ruvC"
                     /locus_tag="VFY27_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5764523.1"
                     /translation="MTERHRILGIDPGSRLTGYGVLDFIDDRAFYVASGSVRSIEGEF
                     AARLRLIFRSVTEIVAEFRPDAVVIESIFVHRNAMSALKLGQARSAALCATFGLDIGV
                     HEYAPREIKQAVAGSGAATKVQVQHMVMSLLQLKGAPSPDAADALAAALCHGHQRRLY
                     SRLAGAAVSAGLR"
     gene            complement(1708..>2117)
                     /locus_tag="VFY27_03095"
     CDS             complement(1708..>2117)
                     /locus_tag="VFY27_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5764524.1"
                     /translation="VRHAFSKHGGNLGADGSVSYLFNHVGLLSYPPGSNEDAIMSAAI
                     DAGAEDVIVDEDKSIQVLTDPVEMEAVRDAMQAAGLPPGDAELTMWAATTTELGFEEA
                     SSMVRLLEALEDLDDVQNVYSNADIRDDVLSQL"
BASE COUNT          383 a          698 c          637 g          399 t
ORIGIN      
        1 ccggccgagc ggtgggcaat cgcctccagt tccgctgtcg agtagaactc cagccgttgc
       61 acgataccga atcggtcgcg cagcggcgag gtcaggaggc ccgcccgagt ggtggctccg
      121 acgagggtaa acggcgcaag gtccagcttg atggaacgtg ccgcgggccc ttcgccgata
      181 acgatgtcga gctggaaatc ctcgagtgcc ggataaagca cttcctcgac gacagggctc
      241 aggcgatgga tctcgtcgac gaacagcacg tcattcggtt cgagattcgt cagtatcgcc
      301 gcaaggtccc ccggccgctc gagtaccggc cccgacgtct ggcgcaggtt cactcccatt
      361 tcgtaagcaa tgatgtgagc cagcgttgtc ttgccgaggc ccggcggtcc gaaaataaga
      421 acgtggtcga gcgcctcccg ccgattgcgc gcagcggtga tgaaaatctc catctgttgc
      481 ctgaccgccg gctggcccac gtagtcggcc agcgaccttg ggcggatggc gcggtcgaac
      541 acgcggtcct cggggcgcgg cgcggcactg gccaatcggt cccgttctat ggcagtcatc
      601 gctcagccga ccgcctggcg caatgccgag cgaatgatgt cttccgcgga cttgtcattg
      661 agatccagtt gccccattag tcgcttgacc tcctgcggct tgtaacccag cgccaccagc
      721 gcgtcgaacg cttcactgcg cgcatccgcc cgcatgacgc ggccggccgc gcttttcggc
      781 attgtcgcct gctggttgat acgatcgcgc agttcgataa tcagtctctc ggcggttttc
      841 cgcccgacgc cggggatttt taccagcatg gcactgtctt cgtgcagcac acagcgatgg
      901 aagtccgcca cactcatgcc ggacaggata gccagggcca tctttgcacc gacaccactg
      961 acccgcagca aactgcggaa caggtgcttc tcctcctccg tggcgaagcc aaacagcgac
     1021 tgcgcgtctt cccggacctg caggtgcgtg tgcaggaata tgtcgcttcc aacctgcggc
     1081 aactcgagaa acgtcgacat tggcgtctcg atctcgtagc cgacgcctgc acattcaacc
     1141 agtatccttg gcggctgttt cagtgtgagc cgtccgcgca ccgagccgat catcgcaggc
     1201 ctgcgctgac ggcagcgcct gccaggcgcg agtacagccg ccgctggtgg ccgtgacaaa
     1261 gtgcggcagc cagcgcatcc gcggcatcgg gcgacggcgc acccttgagc tgcagcagcg
     1321 acatgaccat gtgctggacc tggaccttcg ttgcggcacc gctgcctgcg actgcctgct
     1381 tgatctcccg cggcgcatat tcatgaacgc caatgtcgag cccgaacgtc gcgcagagcg
     1441 cggcagaccg ggcctgtccc agcttcagcg cgctcattgc gttgcgatgc acgaagatgc
     1501 tctcaatgac caccgcgtcc ggcctgaatt ccgcaacgat ctcggtcacc gaacgaaaga
     1561 tgagccgcag gcgcgctgcg aactcacctt cgatgctgcg cacggagccg ctggcgacat
     1621 agaacgcgcg atcgtcgatg aaatccagca cgccgtagcc tgtgaggcgg gagccgggat
     1681 ctatccccag tatgcgatgt cgctcggtca cagttgggac agtacgtcgt cgcggatgtc
     1741 ggcgttgctg tagacgttct gtacgtcgtc caggtcttcg agggcttcga gaagcctgac
     1801 catcgagctt gcttcctcga atcccagttc ggtcgtcgtt gcagcccaca tcgtcagctc
     1861 cgcatccccg ggcggcaagc ctgctgcctg catcgcatcg cgaacggctt ccatttcgac
     1921 cgggtccgtc aggacctgta tcgacttgtc ctcgtcaacg atcacgtcct ccgcgcccgc
     1981 gtcgatcgcc gccgacatga tggcatcctc gttgctgccc gggggatacg aaagcaagcc
     2041 gacgtgattg aacaggtagc tgaccgagcc gtcggcgccg agattcccac cgtgcttgga
     2101 aaaagcatgc cgcacat
//
LOCUS       DASUOM010000156         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_21221,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000156 DASUOM010000000
VERSION     DASUOM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21221"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..940)
                     /locus_tag="VFY27_03100"
     CDS             complement(170..940)
                     /locus_tag="VFY27_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5764525.1"
                     /translation="MPTSTHEVWTGLAWGAVSSVGLLIGAAAGSFFRMPHHAIAMAMS
                     VGAGLLLAGVSFKVAADAIRIAGPAAAALSLLIGAAVFSASNALLARFGAAHRKRCGD
                     CMQQPIESEQPGSGVAIALGNALDGLPEALVLGIVLRERIAPIALVVAFSLGNFPEAL
                     SSAAGMRTAGRSHAYIVFMWSAIAIGAAVAIALGYVGFTALDDVWPPRLQAFGAGALI
                     AMTAETMIPEAFHNSPRFSGLLAACGFALLLLVDAAAR"
     gene            complement(1041..2105)
                     /locus_tag="VFY27_03105"
     CDS             complement(1041..2105)
                     /locus_tag="VFY27_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5764526.1"
                     /translation="MKAEEHRDEPSLHGHSARPSRFAERSIPTPELIGSNPRFRAVLE
                     DARTVAAADCAVLIQGETGTGKELIARAIHEASARRHQHYVAVNCAAIPTALLESELF
                     GYERGAFTGAIASKMGRFQAADHGTLFLDEIGELPIELQPKLLRAIQEQEFERLGSTH
                     TTRVDVRIVAATNQSLEQMVAQRRFRMDLYYRLNVFPITVPPLRERTGDIPALVHHFV
                     RTHASRNRKSIACVPDEVMTAFMEYDWPGNIRELQNFIERSVILTKGVELQAPVTELV
                     NKRLPIPGVRTLADAERAHIISTLRKTNWAVGGRNGAAARLGLVRTTLIAKMRKLGIS
                     RDTADQFGEALDPGERVLVC"
     gene            complement(2128..2391)
                     /locus_tag="VFY27_03110"
     CDS             complement(2128..2391)
                     /locus_tag="VFY27_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764527.1"
                     /translation="MIEPDVTVLRISGRIAAQELEVLRAAVGEESGDVVIDLKDVGLI
                     DGDAVGFLAIIEANGGALRNCPAFIREWVNRERAQTRPDQSEA"
     gene            complement(2549..>2732)
                     /locus_tag="VFY27_03115"
     CDS             complement(2549..>2732)
                     /locus_tag="VFY27_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5764528.1"
                     /translation="SVSTADDARGEREMIEAALVESRGRVAGPSGAAAKLGIPASTLE
                     SKIKAFKIRKSQFKFG"
BASE COUNT          489 a          919 c          812 g          512 t
ORIGIN      
        1 cgaggacagg tctgggttgc gccacgggcc gtcagcggcg ataaccccgt cggcttcagt
       61 tggtccccgc ctgggccagg atgtccctgc cacgacgaca cgattcctgt tcgcggctgg
      121 cctgcacgag ggcggcgatc aaggcgctcg cctgaaccta ccaggatctc taacgtgccg
      181 cagcgtctac gagcagcagc agggcgaaac cacacgcagc gagcaagccg gaaaaacgcg
      241 ggctgttgtg gaaggcctcg gggatcatcg tttccgcagt catcgcgatc agtgctccag
      301 ctccaaacgc ctgcagtcgc ggtggccaca cgtcatcgag cgcagtgaac ccaacatacc
      361 ccaacgcgat tgcgaccgct gccccgatgg cgatcgcact ccacatgaaa acgatgtagg
      421 cgtggctgcg gccggcagtc cgcatccccg cggcgctgga taaggcctcg ggaaagttgc
      481 ccagcgagaa cgcgactacc agtgcgatgg gcgctatccg ctcgcgcaga acgattccca
      541 gcaccagtgc ctccgggagc ccgtcaagcg cgttgccgag cgcgattgct actccgctgc
      601 ctggctgctc cgattcgatt ggctgctgca tacagtcgcc gcatcgtttg cggtgagcgg
      661 caccgaaccg cgcgaggagg gcgttgctcg cgctgaacac ggctgcgcca atcagcagtg
      721 acagcgccgc cgccgccggc ccggcgatcc ggattgcgtc agcggccacc ttgaaggaca
      781 caccggcgag cagcagtccc gcccccaccg acatggccat cgcgatcgcg tggtgcggca
      841 ttcgaaagaa cgacccggcc gccgcgccga tcaacagccc gacagaggac actgcgcccc
      901 acgccaggcc ggtccacact tcatgagtac tcgtgggcat atgcactcag gatggttgcc
      961 gcctttggag aagtccttgg atgcggccgg gcagctgcag ccggttcggc tccgcttccc
     1021 ccggaatcag ttccgtcgat tcagcacacc aggacgcgtt cacccggatc gagggcctca
     1081 ccgaactggt cggcggtgtc ccgagaaatc ccgagctttc gcatcttcgc gatcagagtt
     1141 gtccggacca gcccgagtct ggctgccgcg ccattgcgtc cgccaaccgc ccaattcgtt
     1201 ttacgaaggg tcgaaatgat gtgcgctcgt tccgcgtccg ccagagtgcg cacgcctgga
     1261 atcggcaggc gcttgttgac gagttcggtg accggcgctt ggagctccac tcctttcgtc
     1321 agaatgaccg agcgctcgat gaagttctgc agctcccgaa tgttgccggg ccaatcgtat
     1381 tccatgaacg ccgtcatcac ttcgtcgggg acgcatgcga tcgatttacg attgcgagag
     1441 gcatgagtac ggacgaaatg atgaacgagc gcgggaatgt cgcctgtccg ttcgcgcagc
     1501 ggcggcaccg tgatcggaaa cacgttcaac cgatagtaca ggtccatccg gaatcgccgt
     1561 tgtgcgacca tctgctccag actctggttc gtggccgcga cgatccgcac atccacgcga
     1621 gtggtgtggg tgctcccgag ccgttcgaat tcctgctcct gaatggctcg caacagcttg
     1681 ggctgtagtt cgatcggcag ttcgccaatt tcgtcgagaa agagggtgcc gtgatcagcc
     1741 gcctgaaagc ggcccatttt cgacgcgatc gctcccgtaa aggcgcctcg ctcatacccg
     1801 aacagctcgc tctccagcaa tgcggtagga atggccgcac agttgaccgc cacatagtgc
     1861 tggtgccggc gggcgctggc ctcgtgaatc gctcgtgcga tgagctcctt gccagtcccg
     1921 gtttcgccct ggatcaggac ggcgcagtcc gccgctgcca cagtcctcgc gtcttcaagg
     1981 acagccctga atcgcggatt ggatccaatg agttcaggag tcggaattga tctctctgcg
     2041 aaccgactcg gtctcgcgct atgcccatga agcgatggct cgtcacgatg ctcttcagcc
     2101 ttcatcgccg tgctcccttg ctcgtgctca cgcttccgac tggtccggcc tcgtctgcgc
     2161 ccgctctcga ttgacccatt cccggatgaa ggccggacag tttctgagtg cgccgccgtt
     2221 ggcttcgatg attgccagga acccgacggc gtccccatcg atcagaccga cgtccttgag
     2281 atcgatgaca acgtcgccgc tctcctcccc gaccgcggca cggaggactt ccagctcttg
     2341 cgctgcaatc cggccgctta tgcgcaagac cgtcacatct ggttcaatca agcggtcgac
     2401 tctgcaagtc attgcgccgt cgcccctctg acagggcaaa cctcgcgccg tcgcaatgat
     2461 tgaaaagacc cagaacacgt gtgccgcttt gccagcggcc gtctgcgaat ctcgcgaacg
     2521 ccgcgaatct cgcgattccc gcgagtcctc agccgaactt gaactggctc ttccggattt
     2581 tgaaggcttt gatcttggat tccagcgtcg acgcgggaat gcccagcttt gccgccgcgc
     2641 ctgatggccc ggccacccgt ccccggctct cgacgagggc cgcctcgatc atctcccgct
     2701 cgccgcgcgc gtcgtcggcg gtcgacacgg ac
//
LOCUS       DASUOM010000157         5738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1112056,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000157 DASUOM010000000
VERSION     DASUOM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112056"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="VFY27_03120"
     CDS             <1..1796
                     /locus_tag="VFY27_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764529.1"
                     /translation="PECAPRCAEISTARVEVDANTLSIELEVHAFENVAVPLPGSLQG
                     WRPESVQLDGSAARVYRHADQALWIRAPGGTHQVTLQGPLPPVDSLEVPFPAAPRVIT
                     AEASGWSISGVRDRRLVSGSLQLAKLQAAGEDASSRWESARFPVFARVERLVELDLDW
                     RVTTQVVRVAPQQGAFTLDVPLLEGEAIVSGDFQVRNGKVLVSMGSGQRAVSWESTLP
                     RTSPLTLRAESAPWKEVWRFGVSSIWHVGFDGVPESEPESAVSGARVLEFYPRAGEAL
                     TVEAERPAASGGTSLAFDSVSLRSEFGARSRSVALDLAYRSTRGAQHNIRLPEEAEVQ
                     SVTIDGRLESLRPGDGMLSLPILPGEHRIEVRWREEVAAGVRAETPAVDIAAPASNIQ
                     LGIELPRSRWILATSGPRLGPAVMYWSELAALILAAVILGRIGLTPLSTRHWLLLAIG
                     FSTFSWAALALVALWLLVMGARERLIGNLSWWRFDALQLLLAALSVAALATIVVTVPS
                     GLLGTPDMHVVGNGSWGNSLNWFADRSEGLLPAATVISLPIWVYKVLILAWALWLSFA
                     LLRWLPWAWSCFVSQGLWRSRAPAPGATGNR"
     gene            complement(1862..5074)
                     /locus_tag="VFY27_03125"
     CDS             complement(1862..5074)
                     /locus_tag="VFY27_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX5764530.1"
                     /translation="MSGTGPIVTLSPRDYDAVLFDLDGVLTQTATVHAAAWKRLFDDF
                     LSEHAARSGEEFVPFDIKADYLQYVDGKPRYDGVANFLASRGIELPLGNPADGPDERT
                     VHGLGNRKDGYFNEHLKRHGVLPYEPSIELVRSLRKEDIRTAVVSSSNNCAAVLDAIG
                     IAHLFEVRVDGIDVTRLGLRGKPAPDAFNEAARRLRVEPSRSVVVEDAISGVAAGRAG
                     GFRLVIGVDRLAQSQALREAGAHIVVTSLDQVQVAAEPVSAWSLQFEGFDPAREGMRE
                     SLCALGNGYFETRGAALWSQADGIHYPGTYIAGAYNRLQTHVAGRTVENEDLVNFPNW
                     LSLKFAIGDDWFDERTVNILSYRQELDLRHGTLLKSILFEDSAGRRTTLAERRLVSMA
                     DMHMGAVELTLTASNWSGSLTVCSAIDGRIVNSGARLYREFNNMHLEQLESMATDEDV
                     LCLEVRTSQSRLQVAQAVRTRVLRNGSVEAPPRKVVEEPAYVAHEFTVELEQDVPLVL
                     EKVLAMYTSRDHAISEAGIAACKAIARAGSYDDLLRAHVLAWMHNWRRFDIHVRPSSA
                     RFNLNVPMLLRLNILHLLQAVSLNSIGLDIGVPARGWTGEAYQGHIFWDELFIFPFLT
                     FRTPEITRSLLMYRYRRLNEARAAAKDAGFEGAMFPWQSGSDGQEETQELNLNHRSRR
                     WIPDNSWLQRHVGSAVAYNVWNYFQITYDTEFLQFYGAELILEIARFWSSAAHLNEQT
                     GRYEINGIMGPDEFHEGYPDSPVPGLKNNAYTNIMAAWVLCRGLEVLEVLPETRRSEL
                     VRSLGLTEEETSRWQDISRRLFVPFHENGIISQFEGYEKLAELDWSSYRLRYGNIQRL
                     DLILEAENDTGNRYKMNKQADVLMLFYLFSAKQLAELFERLGYPFEYDTIPKNVDYYD
                     ARSTHGSTLSRVVHSWVLARSDRPRAMKYFAEALQSDVADIQQGTTAEGIHLGAMAGT
                     VDLLQRVTTGIEVSGDVLRLDPQLPRELERIDMRVRYRGHALDLRITSDTLTIRGRER
                     GPAPVNLAFRDDVFAFDGLETRVFDLATANESRSRRAGTG"
     gene            complement(5124..5495)
                     /locus_tag="VFY27_03130"
     CDS             complement(5124..5495)
                     /locus_tag="VFY27_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5764531.1"
                     /translation="MAGYVTNIEQDTLENGDYRRVLFTGKNTQLVLMTLQPGEEIGRE
                     VHEEHDQFIRVEAGTGVVSLNREEHKLGDGSAVVIPAGVEHNVVNTSPDEPLRLYTLY
                     SPPEHPDGTVHKTKADEPEEH"
BASE COUNT         1012 a         1783 c         1820 g         1123 t
ORIGIN      
        1 cgccggaatg cgcgcctcgc tgtgccgaaa tttcgacggc gcgggtggag gtcgacgcca
       61 acaccctgtc gatcgaactg gaggtgcacg cctttgagaa tgtcgcggtg ccccttccgg
      121 gttcgttgca gggatggcgg ccggagagcg tgcaactcga cggatcggct gcccgggttt
      181 accggcacgc ggaccaggcg ctctggattc gtgcgcccgg cggtacgcac caggtgacgc
      241 tgcaaggacc gctgcccccg gtggacagcc ttgaagtgcc cttccccgca gcgccgcgcg
      301 tcatcactgc agaagcctcc ggctggtcga tctcgggcgt ccgcgaccgg cggctcgtct
      361 cggggtccct gcaactggca aaactgcaag ctgcaggcga ggacgcatcg tcgcgctggg
      421 agagtgcccg tttcccggta ttcgcgcggg tcgaacggct ggtagaactc gatcttgact
      481 ggcgggtgac aacacaggtc gtgcgggttg cgccgcaaca gggtgcattc acgctcgacg
      541 tcccgctcct cgagggtgaa gcgatcgtca gcggcgattt ccaggtgcgt aacgggaagg
      601 tgctcgtgtc gatgggttcc gggcaacgcg ccgtcagctg ggaatcgaca ttaccgagga
      661 catctccgct cacgctcagg gccgagagcg ctccatggaa agaagtctgg cgcttcggcg
      721 tcagtagtat ctggcacgtg ggctttgacg gcgtgccgga gagcgagccc gaatcggctg
      781 tgtccggcgc gcgcgtgctc gagttctacc cgcgtgcggg ggaggctctc accgtcgagg
      841 cggagcgccc ggcggcaagc ggcggcacgt cgctggcgtt cgacagtgtc tcgctgcgca
      901 gcgaattcgg cgcgcgttcg cggagcgtcg ccctcgatct cgcataccgc agcacgcgag
      961 gtgcgcaaca caacatccgg cttcccgaag aagccgaagt acagtccgtc accatcgacg
     1021 gtagactgga atcgctgcgg ccaggcgacg gcatgctgag cctgccgatt ctcccgggcg
     1081 agcaccgcat cgaggtgcga tggagggagg aagtggccgc tggcgttcga gcggaaactc
     1141 ctgccgtgga tatcgcggca ccggcgagca atatccagct cggcatcgag cttccccgaa
     1201 gccgctggat actcgcgacc tcgggaccgc gactcggtcc ggcagtgatg tactggtcgg
     1261 aactcgcggc actcatcctg gcggcggtca tcctcggacg catcgggctg acgccgctct
     1321 cgacccgtca ctggctcttg ctggcgatcg gtttcagcac gttctcatgg gcggcgctgg
     1381 ccctggtggc gttgtggctg ctcgtgatgg gtgcgcgaga gcgattgatc gggaacctgt
     1441 cctggtggag gttcgatgct ttgcagctgc tcctcgcggc cctgtcggtc gcggcgcttg
     1501 cgaccatcgt cgtaacggtt ccttcgggac ttctcggcac gccggacatg catgtcgtgg
     1561 ggaacggctc ctggggtaac tcgctcaact ggttcgccga ccggagtgaa gggctgcttc
     1621 ccgctgcaac ggtgatttca ttgcccatat gggtctacaa ggtgctcatc cttgcctggg
     1681 cgctgtggct cagctttgcc ttgctgcgct ggttgccatg ggcgtggtca tgcttcgtca
     1741 gccagggact gtggcgttcg cgagcaccag cacccggtgc gacaggcaac cgatgagtca
     1801 tctggaagaa gccatactgc ggcgctttgc cggacgcacg aaccgtccgc gccggagcag
     1861 gtcacccggt tccggccctg cgcgaccggc tttcattggc agtcgcgagg tcaaacaccc
     1921 gcgtctccag gccatcgaac gcgaaaacgt catccctgaa ggccaggttg acaggggcag
     1981 gtccgcgttc gcgaccgcgt atggtcagag tgtcgctggt gattctcaag tcgagcgcgt
     2041 ggccgcgata ccgcacgcgc atgtcgatgc gctccagctc cctgggcagc tgcgggtcga
     2101 gacgaagaac gtcgccgctt acctctatcc cggtggtcac gcgttgcagc aggtcgacgg
     2161 tgccggccat tgcgccgagg tggatgccct cggcagtcgt cccctgctgg atatcggcaa
     2221 cgtcgctttg cagcgcttcc gcgaaatatt tcattgctct cggccggtcc gatcgtgcaa
     2281 gaacccacga gtggacgacg cgagacaggg tggacccgtg tgtcgagcgc gcgtcgtagt
     2341 agtcgacgtt tttcggaatc gtatcgtact cgaacgggta gccaaggcgc tcgaacagct
     2401 ctgcgagctg cttcgctgaa aacaggtaaa acagcatgag cacgtccgcc tgcttgttca
     2461 tcttgtagcg gttgccggtg tcgttctccg cttccaggat gagatcgagg cgctggatgt
     2521 tgccgtatcg aagccggtag ctgctccagt cgagttcggc aagcttttcg taaccttcga
     2581 actggctgat gatgccgttt tcgtggaacg gcacgaacaa gcgtcggctg atgtcctgcc
     2641 agcgcgaggt ttcttcttcc gtcaggccaa gagaacgcac cagctccgag cggcgcgtct
     2701 ccggcagcac ttcgagcacc tcgagtccgc gacagaggac ccacgcggcc atgatgttgg
     2761 tgtaggcgtt gttcttaagg cccggtaccg gcgagtcggg atagccttcg tgaaactcgt
     2821 cgggtcccat gatgccgttg atctcgtagc ggcctgtctg ctcattcaaa tgcgccgcgc
     2881 tggaccagaa gcgcgcaatc tcgagaatca gctcagcgcc gtagaactgc aggaactccg
     2941 tgtcataggt gatctggaaa tagttccaga cgttgtatgc aacggcgctg ccgacatgcc
     3001 gctgcagcca ggaattgtcc gggatccagc gccgtgagcg gtgattcaga ttcagctcct
     3061 gcgtttcctc ctggccgtcg ctcccgctct gccacggaaa cattgccccc tcgaaacccg
     3121 catcttttgc tgccgcgcgc gcttcgttca gccgccggta gcggtacatg agcaacgagc
     3181 gcgtaatctc cggcgtcctg aacgtcagga acgggaagat gaacaactca tcccagaaga
     3241 tgtgcccctg gtaggcttcg ccggtccagc cgcgggccgg cacgccgatg tcgaggccga
     3301 tcgagttcag ggaaaccgcc tgcagcaggt gcaggatgtt aaggcgcagg agcatcggga
     3361 cgttcaggtt gaagcgggcc gatgacgggc gcacgtggat atcgaaccgg cgccagttgt
     3421 gcatccacgc aagcacatgt gctcggagca ggtcatcgta agaaccggcg cgggcaatgg
     3481 ccttgcaggc agcgatgccg gcttccgaga tcgcatgatc gcgagaggtg tacatcgcga
     3541 gcaccttctc cagcacgagt ggaacgtcct gttccagctc taccgtgaat tcgtgggcca
     3601 cgtatgcagg ttcctcgacc accttgcgcg gtggtgcctc gacgctgccg ttgcgcagga
     3661 cccgcgtgcg cacggcctgt gccacttgca gcctcgactg ggacgtccgg acctcgaggc
     3721 acagtacatc ctcgtcggtc gccatgctct cgagctgctc gagatgcatg ttattgaact
     3781 cccggtaaag cctggcgccg gaattgacga ttcgcccgtc aattgcggag caaacggtca
     3841 gcgacccgga ccagttcgag gcggtcaacg tcagttctac cgcacccatg tgcatgtcgg
     3901 ccatcgacac cagtcgtcgc tccgccagcg tggttctgcg acccgcggaa tcctcgaaga
     3961 ggatcgattt cagcaaggtg ccatgtctca ggtcgagttc ctggcggtag gagaggatgt
     4021 taacggttcg ttcgtcgaac cagtcatcgc cgatagcgaa tttcaacgac agccagttcg
     4081 ggaaattcac caggtcttcg ttttcgaccg tgcggccggc aacgtgcgtc tgcaggcggt
     4141 tgtacgcgcc cgcgatatag gtgcccggat aatgaatacc gtctgcctgc gaccacagag
     4201 ctgcgccccg agtctcgaag tagccgttgc cgagcgcgca aagcgactcg cgcatgcctt
     4261 cgcgggcagg atcgaacccc tcgaactgaa gcgaccacgc ggaaaccggt tcggccgcga
     4321 cctgcacctg gtcgagactg gtcaccacga tatgcgcacc ggcttcgcgc aacgcctgcg
     4381 actgggcaag cctgtccacg ccgatcacca ggcggaagcc ccctgcccgc ccggcggcca
     4441 cgcccgagat cgcgtcttca acgaccacgc ttctcgaggg ttctacccgg agccgcctcg
     4501 ctgcttcatt gaatgcatcc ggggcgggtt tgcccctgag cccaagccgc gtcacgtcaa
     4561 ttccatcgac gcggacttcg aacaggtggg cgatgccgat cgcatccagg acggcagcgc
     4621 agttgttgct cgacgatacc acggcagtac ggatgtcctc cttgcgcagg gagcgcacca
     4681 gctctatgga cggctcatac ggcaagacgc catgcctttt caggtgctcg ttgaagtagc
     4741 cgtccttgcg gttgccaagt ccatgtaccg tccgctcgtc cggtccgtcg gcgggattgc
     4801 ccagcggcag ttcaatgccg cgcgacgcga gaaagttggc cacgccgtca tagcggggct
     4861 tgccatccac gtattggagg tagtccgctt tgatgtcgaa gggtacgaac tcttcaccgg
     4921 atctggcggc atgctcgctg agaaagtcgt cgaacaacct cttccaggca gcggcatgaa
     4981 cggtagccgt ctgtgtcagc accccgtcca ggtcgaacag caccgcgtca taatcccgcg
     5041 gggacaacgt gacaatcggc ccggtgccgc tcaacgccgc ggctcgctga ccgcttcagt
     5101 acacaggctc acgccggatg gccttaatgt tcttcgggtt cgtcggcttt cgtcttgtgt
     5161 acggtgccgt ccggatgctc cggagggctg tacagggtgt agaggcgtaa tggttcatcc
     5221 ggggacgtgt tgacgacgtt gtgctcgacg cccgcaggga tgacgaccgc ggagccgtca
     5281 ccgagtttat gttcctcccg attcaggctt acaacccccg tccctgcttc cacccggatg
     5341 aactggtcat gctcctcgtg aacttcccgc ccgatttcct ctcccggctg aagcgtcatg
     5401 agaacaagct gcgtattctt gccggtaaaa agcacgcgtc ggtagtcgcc gttttccagc
     5461 gtatcctgtt cgatgttcgt cacgtatccc gccatgggtc acctcggttg gttcaagtgg
     5521 gtttagttgc caggaaggcc gccagcgcgt tacagcctgg tagaaggaaa cgcctcagcg
     5581 taagcaggta aataacggca agggctgaag ggtcaccttg gccgccgcgc cggcaggcgc
     5641 gacggccgga tgacaaaaag tatacacgcg ctccccttgg acaggctaat cgatcacctc
     5701 gtagtgacgc accttctctg gcatctctac gaagtact
//
LOCUS       DASUOM010000158         5526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1372418,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000158 DASUOM010000000
VERSION     DASUOM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372418"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /gene="fusA"
                     /locus_tag="VFY27_03135"
     CDS             complement(<1..1317)
                     /gene="fusA"
                     /locus_tag="VFY27_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5764532.1"
                     /translation="MARTTPIERYRNIGIMAHIDAGKTTTTERVLFYTGVSHKMGEVH
                     EGAAVMDWMEQEQERGITITSAATTCFWQGMDKQFEEHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVTVLCSVGGVEPQTETVWRQGNKYKVPRLIFVNKMDRAGANFLRVIKQVKD
                     RLGATPVPVQLPIGAEENFEGVVDLVTMKAVYWDDATMGMNFELREIPESMLAEAATW
                     REKMVEAAAEANEELLDKFLGDGELDEEEIRKGLRIRTLAGEIVVAMCGSAFKNKGVQ
                     AMLDGVVRYMPSPADVPPIKGLLEDGSEGFRHADDSEPFSALAFKIATDPFVGNLTFF
                     RVYSGVLNSGDMVYNPIKGKKERIGRILQMHSNDRKEIKEVCAGDIAAAVGLKDVTTG
                     DTLCELKNIITLEKMEFPEPVIAVAVEPKTKADQEKMGLALQKLAKE"
     gene            complement(1352..1831)
                     /gene="rpsG"
                     /locus_tag="VFY27_03140"
     CDS             complement(1352..1831)
                     /gene="rpsG"
                     /locus_tag="VFY27_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5764533.1"
                     /translation="MSRRSQAPKRTILPDPKFSSDLLAKFMNMIMNDGKKSVAERIIY
                     GALDRISERQTHSDEKALELLEQALENVKPAVEVKSRRVGGATYQVPVEVRPARRQTL
                     AMRWLIDAARNRSEKSMAFRLAHELMDAADNRGTAVKKKEDTHRMAEANKAFSHYRW"
     gene            complement(1938..2309)
                     /gene="rpsL"
                     /locus_tag="VFY27_03145"
     CDS             complement(1938..2309)
                     /gene="rpsL"
                     /locus_tag="VFY27_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5764534.1"
                     /translation="MATTNQLVRSPRSLKRAKSAVPALEGSPQKRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTIRGTL
                     DCAGVSDRRQGRSKYGAKRPK"
     gene            complement(2731..>5526)
                     /locus_tag="VFY27_03150"
     CDS             complement(2731..>5526)
                     /locus_tag="VFY27_03150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947937.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5764535.1"
                     /translation="VVLGLYYMTRSKVFGKGEGMVFSNIDEARRAYENGYAELQARVK
                     VRVPMHDADGETATVTRLVDTTVGRALLSNLLPSGLSFDLVNRDMTKKAISNVINACY
                     RNLGLKKTVVFADQLMYTGFRFATIAGISIGVNDMVVPDEKAAILSIAESEVKEIQDQ
                     YASGLVTEGERYNKVVDIWSRTNDQVAKAMMDKLGSETVEDANGNVVDQESFNSIYMM
                     ADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLDVLQYFISTHGARKGL
                     ADTALKTANSGYLTRRLVDVAQDLVVTEVDCGTRNGVSMAPLVEGGDVVEPLRERVLG
                     RVLAEPVLIPGTDKVAFDAGAMLDEASVARLEELSIDNVLVRSPITCDVRYGVCSQCY
                     GRDLARGHRINIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRSAAVSNIEIKNKGV
                     VKLNNIKTVHHQKGYLIAVSRSGEISVMNEHGRERERYKVPYGATITVTDGASVLPGE
                     IVATWDPHTHPVVTEVGGYLHYEDFVDGVTVTEQIDEFTGLSSVAVLDPKQRGSSGRD
                     LRPMLKLVDDSGKEVCFANTDIPAVYALPTGAIVSMSDGAKVQVGDVIARIPQESSKT
                     RDITGGLPRVADLFEARKPKDPAIMAEYTGTVSFGKETKGKRRLVITDESGESHEELI
                     PKWRHLNVFEGEQVVRGEVIADGEPNPHDILRLQGVEPLSDYLVREIQDVYRLQGVKI
                     NDKHIEVIIRQMLRKVLVADPGESNFLRGEQIDKARGLEVSEQLKVQNKDPLTVEPIL
                     LGITKASLATESFISAASFQETTRVLTEAAVRGLRDDLRGLKENVIVGRLIPAGTGFA
                     EHAERRRTREQDLSDQLRELEEETTARNRLMGEDESEAEDETAVAESAGEDELGQKAG
                     DAGEVEEETEETATSDR"
BASE COUNT          908 a         1778 c         1636 g         1204 t
ORIGIN      
        1 ttccttcgca agcttctgca gcgccaggcc catcttctcc tggtcggcct tggttttcgg
       61 ctcgacggcg acggcgatca ccggctcggg gaattccatc ttctcgagcg tgatgatgtt
      121 cttcagctcg cacagcgtgt cgcccgtcgt gacgtccttg aggccgaccg ccgcggcgat
      181 atcgccggcg cacacttcct tgatctcttt ccggtcgttc gagtgcatct gcaggatgcg
      241 gccgatccgc tctttcttgc ccttgatcgg gttgtagacc atgtcgcccg agttcagcac
      301 gcccgagtag acacggaaga acgtcaggtt gccgacgaac gggtccgtgg caattttgaa
      361 ggcaagcgcc gagaacggct cgctgtcatc tgcgtggcga aagccttccg atccgtcctc
      421 gagcaaccct tttatggggg gcacgtcggc aggcgacggc atgtaacgca ctacgccatc
      481 gagcattgcc tgcacgccct tgttcttgaa ggcagagcca cacatggcaa ctacgatttc
      541 gcccgccagc gtgcgaattc gcaggccttt gcggatctct tcctcgtcga gttcgccgtc
      601 gcccaggaac ttgtctagaa gctcttcgtt cgcttctgcg gctgcctcga ccattttctc
      661 gcgccaggtt gcagcttctg ccagcatgct ttcgggaatc tcgcgaagct cgaaattcat
      721 gcccatggtc gcgtcgtccc agtagacggc cttcatggtg acgaggtcca cgacgccttc
      781 gaagttctct tcggcgccga tcggcagctg caccggcacg ggagtggctc ccagccgatc
      841 cttgacctgc ttgatgacgc gcaggaagtt ggcgccggca cggtccatct tgttgacgaa
      901 gatgaggcga ggcaccttgt acttgttgcc ctggcgccag accgtctcgg tctgcggctc
      961 cacgccgccg acggaacaga gaaccgtcac cgcgccgtcg agcacgcgca ggctgcgctc
     1021 gacttcaatc gtgaagtcga cgtgccccgg cgtatcgatg atattgatgc ggtgctcttc
     1081 gaactgcttg tccatgccct gccagaagca ggtggtggca gcggacgtga tcgtgatgcc
     1141 gcgctcctgc tcctgctcca tccagtccat gacggccgca ccctcgtgta cctcgcccat
     1201 cttgtgcgat acaccggtgt agaaaagaac gcgctcggta gtcgttgtct tgccggcatc
     1261 gatgtgggcc atgatgccga tgttgcgata gcgttcgatg ggtgtcgtgc gtgccacaga
     1321 aatatccttg cgtgattcgg gtacggaagc gctaccagcg gtagtgcgag aaagccttgt
     1381 tggcttctgc catgcggtgc gtgtcttcct tcttttttac ggccgtgccg cgattgtcgg
     1441 cagcgtccat caattcgtgc gccagccgga aagccatgga tttctcgctc cggttgcgcg
     1501 cggcgtcgat cagccagcgc atggccagcg tctgccgccg ggccggcctc acttcgaccg
     1561 gtacctggta ggttgcgccg ccgacgcgcc gggatttgac ctcgacggcc ggctttacgt
     1621 tctccagcgc ctgctcgagc agttccagcg ccttctcgtc gctgtgcgtc tgccgctccg
     1681 aaatacggtc gagcgcgccg tagatgatgc gctcggcgac cgatttcttg ccgtcattca
     1741 tgatcatgtt catgaacttc gccagcaggt cgctggagaa tttcggatcc ggcaggatcg
     1801 tgcgttttgg agcctggctt cttcgtgaca tcgtttgtct cttatgtccc cgccgcggga
     1861 tagagtcggg cggaggggat gctgcgggag tctgttacgg gtctgctttt tacttacctg
     1921 gcgttatttg cctggcgtta cttgggtctc ttggcgccgt acttggaacg gccctgcctg
     1981 cggtcactga cgccggcgca gtcgagcgtg ccgcgaatcg tgtgataacg gacacccggc
     2041 aggtctttta cgcggccgcc gcggatcagt acgaccgagt gctcctggag gttatgaccc
     2101 tcgccaccga tgtagctcgt gacctcgaag ccgttcgtca gccggacacg cgcaactttc
     2161 cgcatcgccg agttcggctt cttcggggtg gttgtgtata cgcgggtgca tacgccccgc
     2221 ttctgcgggc tgccctcgag cgccggcacc gcacttttcg cgcgcttgag cgaacgcggg
     2281 ctccgcacta gttgattcgt tgtggccatg ggccccttta actcagaaag ttcgtgaaac
     2341 aggttcaaaa caaaaacacg cgcagtgatg cgcgcgcacc cacttgccgg accgtatcgt
     2401 aaggtcagct cgcggggttc gaggtacgcg aaggtaagct tctgatccga agaagctttc
     2461 caccgccatc tcgtgttcga ttgtcaggcc cgttcgtggc aggcctgtcg ggtaagggcg
     2521 ggcattgtat tgatgccccg cccggagtgt caagctggtc acgcgctcgc aaatcgcacg
     2581 tcacgcggcc cgatggaacc gtaggagccc acgcccaacg aacgcgatat gcatccgaac
     2641 ccctattgcc tcacggacgg cggatgcgga cgagttagct gatgtcgatc gcactcgtcc
     2701 gcatggcggg ctcctacggt gctcttttcc ctagcggtcg gaggttgcgg tttcctccgt
     2761 ttcctcctcg acttccccgg cgtcgccggc tttctgcccg agctcgtcct cgcctgccga
     2821 ttcggctact gccgtctcgt cttccgcctc ggattcgtct tcgcccatca gccggtttct
     2881 cgccgtggtc tcttcctcca gctctcgcag ctggtccgaa agatcctgct cccgcgtccg
     2941 gcggcgctcc gcgtgttccg cgaagcccgt cccggcaggg atcaggcggc cgacaatgac
     3001 gttctccttc aggccgcgca ggtcgtcccg caggcccctg accgcagctt cggtgagcac
     3061 ccgggtcgtc tcctggaacg aggccgcgga aatgaacgac tccgtcgcca gtgaggcctt
     3121 ggtgattccg agcagaatcg gctcaaccgt cagcggatcc ttgttctgga ccttgagttg
     3181 ctcggagact tccagcccgc gcgccttgtc gatctgctcg ccgcggagga aattgctctc
     3241 gcccgggtcg gccaccagca ccttcctgag catctggcgg atgatcactt cgatgtgctt
     3301 gtcgttgatc ttgacgccct gcaggcggta cacgtcctgg atctcgcgca cgagatagtc
     3361 ggacagcggc tcgacgccct gcaatcgcag aatgtcgtgc gggttcggct cgccgtcggc
     3421 aatgacctcg ccacgcacca cctgttcgcc ctcgaagacg ttcaggtgac gccacttcgg
     3481 aatcagttcc tcgtgactct cgccggactc gtccgtaatg acgagacgac gcttgccctt
     3541 ggtttccttg ccgaagctga ccgtaccggt gtactccgcc atgattgccg gatccttggg
     3601 cttccgcgcc tcgaacaggt cggccacccg cggcaagccg ccggtgatgt cgcgagtctt
     3661 cgacgattcc tgtggaattc gtgcgatgac gtcgcccacc tggaccttgg cgccgtcgct
     3721 catgctgaca attgcgcccg tcggcagagc gtacacagcc ggaatgtcgg tgttcgcgaa
     3781 gcagacttcc ttgccgctgt cgtcgaccag cttgagcatc ggacgcaggt cgcgaccgga
     3841 actgccccgc tgtttcggat ccagcacggc tacgctgctc agtccggtga actcgtcgat
     3901 ctgctcggtc accgtgaccc catcgacgaa atcctcataa tgcaggtagc cgcccacttc
     3961 cgttaccacc gggtgggtgt gcggatccca ggtcgcgacg atttcaccag gcaggacgga
     4021 cgcaccgtcg gtaaccgtaa tcgtggcgcc atagggcacc ttgtaacgct cgcgctcacg
     4081 accatgctcg ttcatcacgg agatctcacc cgaacgcgat acggcgatca ggtaaccctt
     4141 ctggtgatgc accgtcttga tgttgttcag cttcacaaca cccttgttct tgatctcgat
     4201 gttgctgact gccgcggaac gcgacgccgc gccaccgatg tggaacgtgc gcatcgtgag
     4261 ctgagtgccc ggctcgccga tcgactgcgc ggcaatgaca cccaccgctt cgccgatgtt
     4321 gatgcgatgg ccgcgcgcga ggtcgcggcc gtagcactgc gagcaaacgc cgtaacggac
     4381 atcacaggta atcggcgaac gaaccaggac gttgtcgatt gaaagttctt cgagccgtgc
     4441 cacgctggct tcgtccagca tcgcgccggc gtcaaacgcc accttgtcgg tccctggaat
     4501 gaggaccggc tctgccagca cgcgtccgag aacgcgctcg cgcaggggct cgacgacgtc
     4561 gccaccttct accagcggcg ccatgctgac accgttgcgg gtgccgcaat cgacctccgt
     4621 taccaccagg tcctgcgcca cgtcgaccag ccgacgcgtc aggtagcccg agttcgccgt
     4681 cttcagtgcg gtatcagcca ggcctttgcg cgcgccgtga gtcgagatga agtactgcag
     4741 tacgtccagt ccctcgcgga agttggcggt aatcggtgtt tcgatgatcg aaccgtccgg
     4801 cttcgccatg aggcctcgca tgccggcgag ctgccggatc tgcgcggcgg aaccacgagc
     4861 gccggagtca gccatcatgt agatcgagtt gaacgattcc tggtcgacga cgttgccatt
     4921 ggcatcctcg acggtttcgg aaccgagctt gtccatcatc gccttggcta cctggtcgtt
     4981 cgtgcgtgac cagatatcga cgaccttgtt gtaacgctcg ccttccgtca cgaggccgga
     5041 ggcatactgg tcctggatct ccttcacttc gctttcggcg atggacagga tcgctgcctt
     5101 ttcgtcgggc acgaccatat cgttgacacc aatcgagatg ccggcaatgg tcgcgaagcg
     5161 gaagcccgtg tacatcagct ggtcggcgaa gacgacggtc ttcttcagac ctaagttccg
     5221 gtagcaggca ttgatgacgt tggaaatcgc cttcttggtc atgtcccggt tgacgaggtc
     5281 gaagctcagg ccgctcggca gaaggttcga aagcaacgca cgcccgacgg tggtatccac
     5341 caggcgcgtg acggtcgccg tttccccgtc cgcatcgtgc atcggcacgc gaaccttcac
     5401 ccgtgcctgc agctcggcat atccgttctc ataggcgcgg cgggcctcgt cgatgttcga
     5461 aaacaccata ccttcaccct tgccgaacac cttcgagcgg gtcatgtaat agagacccag
     5521 cacgac
//
LOCUS       DASUOM010000159         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_854574,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000159 DASUOM010000000
VERSION     DASUOM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854574"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFY27_03155"
     CDS             <1..300
                     /locus_tag="VFY27_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764536.1"
                     /translation="QDENADARVLEAIFRAEKPAEGRAVVGNAVSEDGNYAVYSVAAV
                     IPGRPESIPLEQRDTEKERLTQQAGAADYTAVVLDLERQADIVIADDALAEPEFQ"
     gene            344..1648
                     /locus_tag="VFY27_03160"
     CDS             344..1648
                     /locus_tag="VFY27_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5764537.1"
                     /translation="MKKPALHWQIIGAIVLAGAVGWAVNRAGGGDPAAPEILGVSYLS
                     IFEYVGALFLNALKMIIVPLILSSIIVGMAGIGSSGNLSSLGGRTLLFYVTTTVAAIL
                     VGLTLINIIGPGYVDGEPAGDLLALEASTDDIAARVEGRGAGDVADLFLRMIPENVVR
                     AAAEGEMLAIIFFALLFGYFMTKLSHDLAEPMYKFWDGVFNVMMRMTEWIMLFAPIGV
                     FGLVAAVVAETGFSAAGPLATFALATLAGLILHATITLPLFLKVFGRVGNPYRIFPAM
                     APAMLTAFSTASSSATLPVTMDCVEKNAGVSNKISSFVLPLGATVNMNGTALYECAAA
                     MFLAQAYGLDLTLGTQFSIVVIALLTSIGVAGVPAASLVAIAIILHAIGLPVEAIGVL
                     MVFDRILDMVRTSINIFGDACAAVIVARMDGERTNLVPGPGA"
     gene            1796..2866
                     /locus_tag="VFY27_03165"
     CDS             1796..2866
                     /locus_tag="VFY27_03165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5764538.1"
                     /translation="MTAIPPDSSEPRICHINLADGFRGGERQTELLVREMAARSWLQR
                     LVVRKDGALVKRCRDVPGLEIAEVVPHPITAAIAGRGCSVVHAHEARAVYSGWLLKRM
                     SHTPYVLTRRIDHAANSSYPRERAYRSAAKVVAISSSIAKTVKAHYPDLECEVVPSAH
                     ADMMNGHANGAANKRVNGKIVVGHIGELDHSHKGQETIIEAARELKDSRPDLHFVLVG
                     HGKDNAKFQTIASGLGNIEFAGFVENVADYLARFDVFIYPSLREGLGSSLLDAMHFGL
                     PIVASRVGGIPEIVEDQVNGLLIPPESPRDLVAAIERVVADAALRDSMRQENRNKAAR
                     FSAARMASSYESIYRSILNGSH"
     gene            complement(2880..3674)
                     /locus_tag="VFY27_03170"
     CDS             complement(2880..3674)
                     /locus_tag="VFY27_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX5764539.1"
                     /translation="MGFMAGKRALIIGLASSRSIAWGIAQAFAREGAELAFTYQNDKL
                     KPRVEGMAAELGSKMTYPLDVASDEEIDATMAALGKEWDGLEVLVHSVGFAPREQLAG
                     TYIESVTREGFKIAHDISSYSLAALAKAALPMMQGRNAAILTLTYLGAVRAVPNYNVM
                     GLAKASLEANVRFLSADLAPRGIQVNGISAGPIKTLAAAGIAGFRKMLAHVEEVSPIR
                     RNVTPEDVGNAAAFLCSDLAAGISSEILYVDGGYNTIGMHLDDDER"
     gene            complement(3806..>5205)
                     /locus_tag="VFY27_03175"
     CDS             complement(3806..>5205)
                     /locus_tag="VFY27_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5764540.1"
                     /translation="DLLARLRAGFSLDVEMNDQIRTHLNYFVRHPEYLDRVFTRAQRY
                     MPYIVEEIEARGLPFELALLPIVESAYDPFAYSHGRAAGLWQIIPGTAKRFGIRQNWW
                     YDGRRDVVDSTEGALSYLAYLNELMDGDWLLAVASYNSGEGNVMRSMRRNRSRSLPTD
                     FWNLKLPKETSMYVPKLLALVELVREPSAYGIELPEMLDAPQFAQAEIGGQLDLALAA
                     ELAGVDVDTLYAFNSGFNHWATDPEGPHRLVVPVDVAETFNAALAALPEDERVRWKRH
                     NVRSGETLSEIAKQYRTTIAAIKSANGISGNTIRAGSYLTVPVASKPLTEYSLSAAAR
                     LEKKQNHERKGQRVEHRVRSGESFWSIGRRYGVGTRELAAWNGMAPRDTLSVGQKLVV
                     WAKNSSPTAAPSANGTTRKLHYRVRSGDSLYLIANRFRITISDLVRWNSIDRNKILRP
                     GQTLTMYVDVTRQSG"
BASE COUNT          960 a         1624 c         1618 g         1003 t
ORIGIN      
        1 caggatgaga atgcggacgc ccgcgtactc gaagcgatat tccgggcaga gaaacctgcg
       61 gaaggacgcg cagtcgtcgg caacgccgtt tccgaagacg gaaactacgc tgtctacagc
      121 gtcgcggccg tcataccggg acgtccggaa tcgattccgc tggagcagcg cgataccgag
      181 aaagaacgac tgacgcagca agccggcgcg gccgattaca cggcagtcgt actggacctt
      241 gagcggcagg ccgatatcgt catagcggac gacgcgctgg ccgaaccgga gttccagtaa
      301 cgggttgcgc tggttcctgg cggcctccgg ggggaagaac aacgtgaaaa aacctgcact
      361 gcactggcag atcattggtg ccatcgttct tgccggcgct gttggctggg cggtaaatcg
      421 cgccggtggc ggagatccgg ccgcaccgga aatcctcggc gtcagttacc tgagcatatt
      481 cgagtacgtg ggtgcgttgt tcctgaacgc gctcaagatg atcatcgtgc cgttgatcct
      541 gtcgtcgatc atcgtcggga tggccggtat cggatcgagc ggaaacctta gctcactggg
      601 cggtagaacg ctgttgttct acgtcaccac gaccgtcgcc gcgatactcg taggcctgac
      661 gctgataaat atcatcggcc ccggctatgt ggacggtgaa cctgccggtg acctgctcgc
      721 gctcgaggcg tcaaccgacg atatcgcggc gcgcgtcgaa ggccgcggtg ccggcgatgt
      781 cgcggacctg ttcctgcgca tgattcccga aaacgtcgtg cgggccgctg cggaaggaga
      841 aatgctcgcc atcatcttct tcgcgttgtt gttcggctat ttcatgacaa agctaagtca
      901 cgatcttgcc gagccgatgt acaagttctg ggacggcgtt ttcaacgtga tgatgcgcat
      961 gaccgaatgg atcatgctgt tcgcgccgat cggcgtcttt ggactcgtcg ccgccgtggt
     1021 tgccgagaca ggattcagcg cggcaggtcc gctggcgacg ttcgccctgg ccacgctggc
     1081 gggactgatc ttgcacgcaa ccatcacgct gcccctgttc ctgaaagtgt tcggcagggt
     1141 gggtaatcct taccggattt ttcctgccat ggcgccagcc atgctgaccg cattctccac
     1201 ggcatcgtca tccgcgaccc tgccggtcac gatggactgc gtcgagaaga atgcaggcgt
     1261 gtcgaacaag atttccagct tcgtattgcc gctcggcgcc acggtcaaca tgaacggcac
     1321 ggccttgtat gaatgcgcgg ctgcgatgtt tcttgcgcag gcgtacgggc tcgacctgac
     1381 tttgggcaca cagttttcca tcgtcgtgat tgcgctgctg acttcaattg gcgttgccgg
     1441 agtgccggcg gcatccctgg ttgcgatagc gatcatcctg cacgccatcg gcctgcccgt
     1501 ggaagctatc ggtgtgctga tggtgttcga tcgcattctc gatatggtgc ggaccagcat
     1561 caatatcttc ggcgatgcct gcgctgcggt catcgtcgcg cggatggacg gcgagaggac
     1621 caacctggtc cccgggccgg gcgcctgatc catagagcgg gccgcgcttt cacgactcac
     1681 cacgatgtgc cctttgcgct tacaatagcc gccagatccg cgatccgggc tggctcagcc
     1741 ctggcgccca tcgacatgcg accccgcacc atgcgggcca tccgccggta cctgtatgac
     1801 cgcgattccc cctgactcga gcgagccccg gatctgccat atcaacctgg cggacggctt
     1861 ccgcgggggc gaaaggcaga ccgagctgct cgtgagggaa atggccgcgc gctcatggct
     1921 gcaacggctg gtggtgcgca aggatggcgc gctggtaaaa cgttgccggg acgtgccggg
     1981 gctggagatt gccgaagtag tgccgcaccc cataaccgcg gccattgccg gccgaggctg
     2041 cagcgtagtg catgcccacg aggcgcgcgc ggtgtactcc ggatggctcc tcaagcggat
     2101 gtcgcacacg ccctacgtcc tgacacgccg catcgatcat gcagccaaca gctcctatcc
     2161 tcgtgagcgc gcctaccggt cggcggccaa agtggttgcg atttcatcct cgattgcaaa
     2221 gaccgtgaag gcgcattacc cggacctcga atgcgaggtg gtgccgagcg ctcatgccga
     2281 catgatgaac ggtcatgcaa acggggccgc caacaaacgc gtcaacggca agatcgtggt
     2341 tggccacatc ggtgagctcg atcacagcca caaagggcag gaaaccatta tcgaagctgc
     2401 gcgggagctg aaggactctc gtcctgacct gcacttcgtg ctcgtcgggc acggcaagga
     2461 caacgccaag ttccaaacca tcgcgagcgg gctgggcaac atcgagtttg caggttttgt
     2521 cgaaaacgtc gcggattacc tcgcgaggtt cgacgtattc atctacccgt ccctgcgcga
     2581 agggctgggc tcgtcgctgc tggacgcgat gcacttcggg ctaccgatcg tcgcctcccg
     2641 tgtcggaggg ataccggaga tcgtggagga ccaggtcaac ggcctgttga taccgccgga
     2701 aagcccccgc gatcttgtcg cggcgatcga acgcgtcgtc gccgacgcgg ccctccgcga
     2761 ttcgatgcgg caggagaaca ggaacaaggc cgcccggttc agcgcagctc gcatggcctc
     2821 atcctacgaa tcgatctacc gctcgatcct gaacggcagc cattgaaaaa ccgctgtgcc
     2881 taccgctcgt cgtcgtccag gtgcataccg atggtgttgt agccgccgtc tacatacagg
     2941 atctcgctcg atatccccgc agcgagatcc gaacacaaga acgccgccgc gttgcccacg
     3001 tcttccggcg tcacgttgcg gcgtatcggc gaaacctcct cgacgtgcgc cagcatcttg
     3061 cgaaaaccgg ctatgcccgc tgccgcgagc gtcttgatcg ggcctgccga aatcccgttg
     3121 acctggatcc cgcgcggcgc aaggtcggca gagagaaaac gcacgttcgc ttcgaggctc
     3181 gccttcgcca gtcccatgac gttgtagttc ggcaccgcgc gaacggctcc aaggtaggtg
     3241 agcgtcagga tcgccgcgtt tcgcccctgc atcatcggca gcgcagcctt cgcgagcgca
     3301 gcgaggctgt agctcgagat atcatgcgct atcttgaaac cctcgcgcgt cacgctttcg
     3361 atataggttc ccgcaagctg ctctcgaggc gcgaaaccca ccgagtgaac gagcacctcg
     3421 agtccgtccc attccttgcc aagtgccgcc atcgtcgcgt cgatttcctc gtcggaggcg
     3481 acgtccagcg ggtaggtcat cttcgaaccg agctccgccg ccatgccctc gacccgcggc
     3541 ttcagcttgt cgttctggta ggtgaaagcg agctccgcgc cctcgcgtgc gaatgcctgt
     3601 gcgatgcccc acgcgatcga gcgactgctc gcaaggccga tgatcaacgc acgcttacct
     3661 gccatgaacc ccatgctgct tcctcgcgat gtgacgttcc ggcgattcta gagtgggaag
     3721 cagggagcca caaccgaagg agatgtgacg tcgggtccgg gaaccttggc aagatgtggc
     3781 gtacgagctc ctacatgcgt ggttgctacc ccgactggcg ggtgacgtcc acgtacatcg
     3841 tcagggtctg gcccgggcgc aggatcttgt tcctgtcgat gctgttccag cgaacgaggt
     3901 cggaaatagt aatgcggaag cggttggcaa taaggtagag agagtcgccg cttcgcaccc
     3961 tgtaatgaag tttgcgggta gtgccgttcg cagaaggggc tgcggtcggc gatgagttct
     4021 ttgcccacac caccagcttc tgcccgacgg acaaggtatc ccgcggcgcc atgccgttcc
     4081 atgcggccaa ttcccgggtg ccgacgccgt agcggcggcc gatcgaccag aaactctcgc
     4141 cggagcgtac gcgatgctcc acgcgctgcc ccttgcgctc atgattctgt ttcttctcga
     4201 ggcgcgccgc ggcgctcagg ctgtattccg tgagcggctt cgaggcaacc ggcaccgtca
     4261 ggtaggagcc cgcccggatc gtattgccgc tgatgccgtt ggcactcttg atggcggcga
     4321 tcgtcgtgcg gtactgcttt gcaatttcgg aaagcgtctc gcccgaacgc acgttgtgac
     4381 gcttccatcg cacgcgttca tcttccggca acgcagccag ggctgcattg aaggtctcgg
     4441 ccacatcgac cggcacgacc agccgatgcg gcccttcagg atccgtcgcc cagtggttga
     4501 agccggagtt gaaggcatag agcgtgtcga cgtcgacacc ggcgagttcc gcagcaagcg
     4561 cgaggtcgag ttgcccgccg atctcggcct gcgcgaactg cggcgcgtcc agcatttccg
     4621 gcaactcgat gccgtaagca gaaggctccc gcaccagttc cacgagcgcc agcaacttcg
     4681 gcacgtacat gcttgtttct ttcggcagct tcaggttcca gaaatcggtc ggcagggagc
     4741 gcgatcggtt gcgccgcatc gagcgcatca cgttgccttc gccggagttg taggaagcga
     4801 ccgccaggag ccagtcgcca tccatcagct cgttgaggta agcaaggtaa ctcaaggcac
     4861 cttcggtgga atcgacgacg tcacgcctcc cgtcgtacca ccagttttgc cggatgccga
     4921 agcgcttcgc ggtgcccggg atgatctgcc acagtcccgc tgcccggcca tgagagtagg
     4981 cgaacgggtc gtaggcgctc tcgacgatcg gcagcagcgc cagctcgaac ggcaggccgc
     5041 gtgcctcgat ctcctccacg atgtagggca tgtagcgctg ggcgcgggta aacacacggt
     5101 cgaggtactc cgggtgtctc acgaagtagt tgaggtgggt ccggatctgg tcgttcatct
     5161 ccacgtcgag gctgaagccg gcgcgaagcc ttgcaagcag atccc
//
LOCUS       DASUOM010000160        11201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1012372,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000160 DASUOM010000000
VERSION     DASUOM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012372"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFY27_03180"
     CDS             <1..1062
                     /locus_tag="VFY27_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764541.1"
                     /translation="FRSRQAFEAAESGLTAAVSYLGSAGGADKDSDGVVDPVFDTDAD
                     GIGDASSITFADNSSVTVTLAGAFPSFTMQSVGVSDDLTATRTVRSLAATVDTLPNAP
                     DNPLTTRGSVVINGSATVHNPEGHSTIWSGNDVELGANNSTSTNIADPTDPDYPSCMD
                     TSMSCDTTRSSSKVAIGLDVIEHDSSLGNLTPAQMFQNFFGTSMENYRASRVSLEVMA
                     ANANNLASNSASPGMDLGAGEVIWVEGDASISKVTVGCEVPVTGSNVCPGASVNPSIV
                     IVNGNLETSGTPHFYGLLYIIGDMNMSSSTSIMGAAIVSGETSSTSGSLDVWYNSDVL
                     NNVRDNGPLAGAPGSWQDW"
     gene            1095..3002
                     /locus_tag="VFY27_03185"
     CDS             1095..3002
                     /locus_tag="VFY27_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5764542.1"
                     /translation="MNVHTARSQRGFTLTEALVSFAITASGLLVIASFQAGLFSGSAY
                     SKARTEALSLAQQKIEQFRHYTHATDDNFIDDNGDGVMDADGTYNDAPIAGQNAQFSR
                     SWALSTSGQTKKIDVTVSWQDRANEAQSVMLATELAWISPRAGVDQLTELGAPLVPSP
                     TGRAKIGEGNLADYPAEDISQVESAGPDGLSLYQHKENILLVDANDKILLTLLDACLT
                     ADGTCKDFVRIEGTVYVDTSNTSQELSAIRVIAADAAHCERWVPSGTLASPPSTANGD
                     YRYYNYTCYLGGGWHGNIGFITDGGLQQRDKVCQGDPTAVDSWAQPVIALRRAYRGMI
                     SKQGDSTVFYSQGIRDAVTLTGQDFVFTELSPTQTEGIHCMGPDDPMTRQDSSGGLLF
                     KDVPTDFVCLNSDADGDSTPDYLDTYDTSVYDADVFCPFDPTDPPVQNHVISGSVSIV
                     SSNALDLSQFSVVTSDGLGNCAWTSSFAATMDGYRAEYSCNVYDWGSGWAGFVQLKPG
                     SNYIYCPTRTATYSAVTSDLSRSFGCYGVPTVTIEGSIMAASGTSVSSLAITDSDTGY
                     QGSCEILNGTSYSCQAWYGDSDWNGTLTVTGSGYACGATAGVFTFLDYTADGSPYAAN
                     IVMAKSSNKCP"
     gene            3029..3487
                     /locus_tag="VFY27_03190"
     CDS             3029..3487
                     /locus_tag="VFY27_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX5764543.1"
                     /translation="MELHRRKQNGITLIELMIVIVIIGILAAVAYPSYQNSVRKARRA
                     DGAAALTNVQLAQEKLRSSCRFYAQNLAGAANVCGADAAGSTVAAPAASGEGHYAVAL
                     SNASATGYTVTATGTGDQANDKAGGVTCTLVLTVSAANPAGTRTPAGCWD"
     gene            complement(3541..4920)
                     /locus_tag="VFY27_03195"
     CDS             complement(3541..4920)
                     /locus_tag="VFY27_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5764544.1"
                     /translation="MSDADRLQEILACPRCDHAPLEASQSGLRCNGCKTRFPSIAGIP
                     WLFAEPDAALGEWRNRLHFDLQRLSHNSQRLKAELNEGKHLPQTEKRLTLLLQATDQH
                     RQDLQQLLAPVDVQSMHAGYHTHLALRTRLPRDQGLQTYYANVHRDWSWGDDENASSL
                     NEIGQVLREGNRGEKPGHTLVLGGGAGRLAYDIHMSLCASSTVVMDFNPLLLLVADAV
                     TRGKRLRLHEFPIAPRTADDVAVLRQLAAPAPVREGFFLVLGDALRAPFRPGSFDTVV
                     TPWLIDIVTEDLPVLAARINRLLKDGGRWINFGSLAFDHLSYARRYGPEETSEIVARA
                     GFARPAVREETIPYMCSPASRHGRQERVFTFAAAKAADASQPPRHRSLPDWIVTGTEA
                     VPSTPSFQSQALSTRIYSYVMSLIDGRRSIEDMALIMEQQKLMSRSEAVPAIRNFLTR
                     MYDDSQRQA"
     gene            complement(5167..6792)
                     /locus_tag="VFY27_03200"
     CDS             complement(5167..6792)
                     /locus_tag="VFY27_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764545.1"
                     /translation="MSRNRKSRHPDAPQFHGDHRRPVTRREFVAQGFLTGSAFALGGG
                     VMSLFSNPRQAFAALSGDLTPLLTNPCNIATVGAGKIPFICFDLAGGANIAGSNVLVG
                     KEGGQLDFLSTAGYERLGLPGDMVPGLNDPISGLPYANTDFGLAFHSDSAFLRGMLRS
                     MSPARAANLNGAIIPARSDNDTANNPHNPLYGIAMAGADGSIVKLAGSENTDSGGNSM
                     IPATMMNPEFRPTKVDRPSDVTGLVDTGELVGILGPDDATAVMESIYRLSDQKMAVAE
                     TGQQVTAPEVVKEMVRCGYLKAADIADRFGGIPLDPAEDPMIVGPNGIFTDAEFAAGG
                     ADGDEFRKTASIMKLVINGFAGAACIEMGGYDYHGGMRREGEVKDERAGRCMGACIEY
                     AARIGVPLMMYVFSDGSLSSNGAIDTSAEGRGKCEWDSDNSSTAASFFMVYNPNGRAV
                     LRGGTPDQQARHQQIGYMGADASVQRAATPAANNVNLLVNTVVLNYMALHGEEGNFAN
                     ILPNHGLGDTSLRDSMTAFDAIVSGTIGPLNPS"
     gene            complement(6823..9339)
                     /locus_tag="VFY27_03205"
     CDS             complement(6823..9339)
                     /locus_tag="VFY27_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX5764546.1"
                     /translation="MKNATEFAGLLSGGMRSLRIMNGLTMLALVSTLGLNGCGGGAAT
                     TENPITGVTPPTAYSGPPPATEDVQSFKLNLWDNVQASNRCGSCHSDSGGQPPLFARH
                     DDINLAYEAANTVVNLSQPSESRLVERVGEAHNCWLSDNQACADIMTTWIEGWAGATA
                     SGGRQIILQPPAPVEPGSSKNYASASLANFASLVHGPVLTTYCASCHSSQASTSQQPY
                     FAEADVAVAYEAAKPRMDLNVPADSRFVIRLGSEFHNCWNDNCTAAAAEMQAAIQAFA
                     DTVPITALDPTLVNSKALRIVDGTVASGGNRYEGAQIALWEFKTGQGSTVFDTSGIEP
                     AINLTLSGAYEWFGGWGVTINDGKAQGSTAASRKLHDLIRATGEYSIEAWVVPANVTQ
                     EMARIVSYSGGDNVRNFTLQQTLYDYNFRHRSTETDLNGDPPLSTPSAEEVLQASLQH
                     VVATYDVVEGRRIYVNGVLVTEADPVATGSLVDWQDTFALVLGNEADGQGLWQGTFRL
                     VAIHNRVLTPEQIVQNFDVGVGEKFFLLFSIEDIINVPTAYILFEVAQYDSYSYLFTK
                     PHFITLDSAQQPEGIPLQGLRIGINGAEAEVGQAYANMDQALSAALFGELGQPLADIG
                     AVVPLERGPQDDQFFLTFDLLGSESYVRTEDPPLVLTQGDLPVAPRFGVRTFDEINAT
                     MAAVTTVDPKQVDIDMTYQSLRQSLPTIESPEAFLASHQVAIAQLAIQYCDALVDDPA
                     KSAAYFPGFNFNQAPATAFAGADRDLVIDPLIDNMMGLGIQTQPDFTVVRDELGYVTA
                     NGSRPANLVDRLVMGGTADTRSVAKGVCAAVLGSAITLVQ"
     gene            complement(9354..10514)
                     /locus_tag="VFY27_03210"
     CDS             complement(9354..10514)
                     /locus_tag="VFY27_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764547.1"
                     /translation="MRNGVSLQLLIALLFSLVLAGVAAANPREQAKRIHDRLAGVPPT
                     EAVLQSMENAISMNNAHAAATIAMDNPGFYNVTLKNFAAPWTNREQSVFVPLNDYTAT
                     VIGMIRDDVPFNTLLSADIVYVGAGNLGLPPYANSNNAHYEQMEQQGVDLMGGLVQTP
                     QSSVTGLPPAATAGVMTTRAAAEAFFVAGTNRAMFRFTLLNHMCNDLEQVMDVKLPPD
                     RIRQDVSRSPGGDSRLFLNNCIGCHTGMDPMAQAFAYYNFNTDSGSIEYTPGQVQPKY
                     FNNDSNFPFGFVTPDDSWTNYWRSGQNRFLGWDPGLPGSGNGAKSMGEELGNSTAFAS
                     CQVKKVFRAVCLRPPEDAADRAQVGQMVSAFTSSGYQLKQAFADAAVYCMGQ"
BASE COUNT         2133 a         3397 c         3420 g         2251 t
ORIGIN      
        1 tttcgctcgc gccaggcatt cgaggcggca gagtcaggcc tgaccgcggc agtctcttac
       61 ctgggcagcg cgggtggagc cgacaaggac agcgatggcg ttgtcgatcc tgtcttcgat
      121 accgatgcag acggcattgg agatgcaagc agcattacct ttgcagacaa cagcagcgtc
      181 actgtcaccc tggccggcgc gtttccgtct ttcacgatgc aatccgtcgg tgtcagcgat
      241 gatttgacgg caacaaggac tgtcaggtcg ctcgcggcta ccgtggatac actgccgaac
      301 gcaccggaca accccctgac tacccgcggc tcggtcgtaa tcaatggatc ggctaccgtg
      361 cacaatccag agggccattc caccatctgg agcggcaacg acgtcgaact tggagcgaac
      421 aactccacgt cgacgaatat tgccgatccg accgaccccg actatccatc ctgcatggat
      481 acatcgatgt cctgcgatac gacgcgttcg tcaagcaagg tcgcaatcgg ccttgatgtc
      541 atagagcacg actcctcgct tggcaacctg acgcccgccc agatgttcca gaactttttc
      601 gggacgtcga tggagaatta ccgggcaagt cgggtcagcc tggaagtcat ggcggccaat
      661 gcgaataacc tcgcgagcaa ttctgccagc cccggaatgg atctcggcgc tggcgaggtt
      721 atctgggttg agggcgatgc ctcgatctcg aaagttacag tgggctgtga ggttcctgtt
      781 accggcagca atgtctgccc tggtgcctct gtgaaccctt cgatcgtcat cgtcaacggg
      841 aatctggaaa cgtcaggcac gccgcacttc tatggccttc tttacatcat tggcgatatg
      901 aacatgtcct ccagcacgtc aatcatggga gctgcgatcg tcagcggcga aacgagcagc
      961 accagcggca gtctcgatgt ctggtacaac tccgacgtct tgaacaacgt gcgcgacaac
     1021 gggccactgg cgggcgctcc cggcagctgg caggactggt aactcgtgag cgctattgga
     1081 aaaggatctc tggcatgaac gttcacaccg cacgcagcca acgaggcttt acgttaaccg
     1141 aagcactcgt ctcgtttgcg ataaccgcct ccggactgct agtcatagcc agtttccagg
     1201 caggcctctt ttcaggaagt gcctacagca aggctcgcac tgaagcactt tcgctcgcgc
     1261 agcagaagat cgagcaattc aggcattaca cgcatgccac tgatgacaat ttcatcgatg
     1321 acaacggtga cggcgtcatg gatgccgacg gtacttataa cgacgctccc attgccgggc
     1381 aaaatgccca gttctcacgt tcgtgggcat tgtcgacttc cgggcaaaca aagaagatcg
     1441 acgtgacggt atcctggcag gaccgtgcga acgaagctca gtcggtcatg ctggccacgg
     1501 aactcgcctg gatttcacct cgcgcgggcg tagatcaact taccgaactt ggcgcaccac
     1561 tcgttccttc gcccaccgga cgggcgaaga tcggcgaagg caacctggct gattatcccg
     1621 cggaggatat aagccaggtg gaaagcgcag gtcccgacgg cctgagcctt taccagcaca
     1681 aagaaaacat ccttctggtg gatgcgaacg acaagatcct gctgaccctg ctggatgcct
     1741 gcctgacggc tgacggtacg tgcaaggact tcgtgaggat cgagggcact gtttacgtcg
     1801 atacgtcaaa cacgtcgcag gagctttcgg caatccgcgt catcgcggcg gatgcggctc
     1861 attgcgagcg ctgggtaccg agcggcacgc ttgcatcacc gccgtcgacc gcgaacggtg
     1921 attaccgcta ttacaactac acctgttacc tcggtggcgg gtggcacggc aatatcggct
     1981 tcataaccga tggcgggctc cagcaacgag acaaggtgtg ccagggcgac ccgaccgcgg
     2041 tggactcgtg ggcacaaccg gtcatagcac tccggcgcgc ataccgcggg atgatctcaa
     2101 aacagggaga cagcaccgtc ttttatagcc agggaatcag ggacgctgtg acccttaccg
     2161 ggcaggactt cgtctttacc gaattatctc caacgcagac cgaaggcatt cactgcatgg
     2221 gtccggacga cccgatgacc cggcaggatt cgtccggcgg gttgcttttc aaggacgtgc
     2281 cgaccgattt cgtctgcctg aacagcgacg ccgacggcga ttccactccg gactacctgg
     2341 atacttacga caccagcgtc tatgacgccg acgtgttttg cccattcgat cctacagacc
     2401 cgcccgtgca gaaccacgtg atttcgggca gcgtaagcat cgtgtcgtcg aacgcgttgg
     2461 acctgtcgca gttcagcgtc gttacatcgg acggcctcgg gaactgcgcg tggaccagtt
     2521 ccttcgccgc gacgatggat ggctacaggg ccgagtattc ctgtaacgtt tacgactggg
     2581 gaagcggctg ggccggcttt gtccagctga agcccggttc caactacatc tattgcccga
     2641 cgaggaccgc cacctactcc gccgtaacat ccgacctgtc ccggagtttc ggatgttatg
     2701 gcgtaccaac tgtgacaatc gaaggttcga taatggcagc gtccggtact tcggtttcgt
     2761 cgctggcgat taccgactcc gatacaggct accagggcag ctgtgaaatc ctgaatggca
     2821 cgagctattc gtgccaggct tggtacggcg acagcgactg gaacggcacc ttgacggtga
     2881 cgggtagcgg ctacgcgtgc ggtgcgactg cgggcgtctt caccttcctg gattacaccg
     2941 ccgacggcag cccgtacgcg gcaaacatcg tcatggcgaa aagctccaac aaatgccctt
     3001 agccgggatc gaagtgaggc tcaccgaaat ggaattgcac cgaagaaaac aaaacggcat
     3061 cacgctgatc gagctgatga tcgtgatcgt catcataggc atcctcgcgg ccgttgccta
     3121 tccttcgtac cagaattcgg tacgcaaggc gcgacgtgcc gacggggctg ccgcactgac
     3181 gaacgtccag ctggcgcagg agaagctgag atcgagttgc cgcttctatg cgcaaaacct
     3241 tgcgggggcc gccaatgtgt gcggcgcgga cgctgccggc tccacggttg ctgccccggc
     3301 cgcaagcggg gagggccact acgcagtcgc tttgagcaat gcaagtgcga caggatatac
     3361 cgtgacggcg acaggtaccg gcgaccaggc aaacgacaag gccggaggtg tgacctgcac
     3421 cctggttctt accgtcagcg cggccaatcc tgctggaact cgcacgccgg ccggttgctg
     3481 ggattagccg ctgtccctgc tgctgcagca gcccgaggct ccggcggcac tagtccatga
     3541 tcaggcctga cgctggctat cgtcgtacat gcgcgtgaga aagttgcgga tcgccggcac
     3601 ggcttcgctc ctcgacatca gcttctgctg ctccatgatc agtgccatgt cttcgatgct
     3661 gcggcgcccg tcgatcagcg acatgacgta ggagtagatc cgcgtcgaca gcgcctggct
     3721 ttggaatgag ggcgtcgacg gcaccgcttc cgttcccgtg acgatccaat cgggcagcga
     3781 ccggtgccgc gggggctggc ttgcgtccgc agccttcgcc gcggcgaatg tgaataccct
     3841 ttcctgcctc ccgtggcggc tggccgggga gcacatataa ggaatagtct cctcccggac
     3901 tgctggtcgc gcgaagccgg cccgggcaac aatctctgac gtttcttccg ggccgtaccg
     3961 gcgcgcgtag ctcaaatggt caaatgcaag cgaaccgaag ttgatccatc tcccgccgtc
     4021 cttcagcagg cggttgatgc gcgcagctaa caccggcagg tcctcggtga cgatatcgat
     4081 cagccacgga gttaccacgg tgtcgaagct gccgggtcga aacggcgctc gcaacgcatc
     4141 gcccaggacc aggaagaacc cttcccgcac gggagcgggc gccgccagtt gccgcagaac
     4201 cgcaacatca tccgcagtcc gcggtgcgat cgggaactcg tgcagtcgca ggcgcttgcc
     4261 acgtgtcacg gcatcggcaa ccagcaacag caatggattg aaatccatta cgacggtcga
     4321 tgacgcgcag aggctcatgt ggatgtcata ggccagccgg cctgcaccac cgccgagaac
     4381 gagcgtgtgt cctggttttt ccccgcgatt gccctccctc aacacctgcc cgatctcgtt
     4441 caacgacgac gcgttctcat catctcccca ggaccagtcc ctgtgaacat tcgcgtaata
     4501 cgtctgcaga ccctggtccc gcggcagcct ggttcgcaac gccagatgag tgtggtagcc
     4561 cgcatgcata gattgcacat cgaccggtgc cagcaattgc tgaaggtcct gccggtgctg
     4621 gtcggttgcc tgcaacagca gcgtgagccg tttctccgtc tgcggcaggt gctttccttc
     4681 gttcagttcc gctttcagtc gctgcgagtt atgggatagc cgctgcaagt cgaagtgcaa
     4741 ccggtttcgc cattcgccca aagcggcatc gggctccgcg aaaagccacg ggatgccggc
     4801 gatcgatgga aatcgggtct tgcagccgtt gcaccgaagc ccgctctgcg aagcctcgag
     4861 cggcgcgtgg tcgcagcgag ggcaggcgag gatttcctgg agacgatcag cgtccgacat
     4921 gcttgaagat gaaatgtcgt gacgccatgc cggttgatcc tgtaatagcc tgcgcggagg
     4981 cgtgaattat cccgtaatcg gcgatggttt ggcagcgaaa atggaacccg ttgcaggagc
     5041 cacgccagga agcgatgacg atatcaaatt ggggatgcct tgcgggcggc ggatgcgggc
     5101 gcgttcgcta tcgcttatcg ctctcgcccg cattacgggc ttccaggggc cggcgacagc
     5161 gatcgctcag gagggattga gaggccctat cgtgccgctg acgatggcgt cgaaggcggt
     5221 catgctgtcg cgcaggctcg tgtcgccgag accgtggttc ggcaatatgt tcgcgaagtt
     5281 gccttcctca ccgtgcagcg ccatgtagtt gaggaccacc gtgttcacca gcaggttcac
     5341 gttgttggcc gcgggtgtcg ctgcgcgctg cacggatgcg tccgcgccca tgtagccaat
     5401 ctgctggtgc ctggcctgtt ggtccggggt gccgccccga agcactgcgc gcccgttcgg
     5461 gttgtatacc atgaagaacg atgccgcggt cgaagagttg tcgctgtccc actcgcactt
     5521 gccgcgtccc tctgccgagg tgtcgatcgc gccattggag gacagcgagc cgtcgctgaa
     5581 gacgtacatc atcagcggca cgcctattcg cgccgcgtac tcgatacacg ctcccatgca
     5641 gcgccctgcg cgttcgtcct tgacctcgcc ttcacggcgc atgccgccgt gatagtcgta
     5701 gccgcccatc tcgatgcacg cggcgccggc gaagccgttg atcaccagct tcatgatgga
     5761 ggcggtcttg cggaactcgt caccgtccgc accgccggca gcgaactcgg catccgtaaa
     5821 gatgccgttg ggaccgacga tcatcgggtc ctcggcagga tcgaggggga ttccgccgaa
     5881 gcggtcggcg atgtcggcgg ccttcaggta gccgcatcgc accatctctt ttacgacttc
     5941 cggcgcggta acctgctggc ccgtttcagc gaccgccatc ttctggtcgc tcaggcggta
     6001 aatcgactcc atcactgcgg tagcatcgtc cggaccgagg atgccgacga gttccccggt
     6061 gtccacgaga ccggtaacgt cgctcggccg atccaccttg gtcggcctga actccgggtt
     6121 catcatcgtc gccggaatca tcgaattgcc gccggaatcc gtgttctcgg aaccggccag
     6181 tttaacgatc gagccgtccg cgcccgccat tgctatgcca tagagcgggt tatgcggatt
     6241 attggcggtg tcgttgtcgg agcgcgccgg aatgatcgca ccgttcaggt tcgccgcccg
     6301 tgccgggctc atgctccgga gcatgccgcg aagaaaagca ctgtcggagt ggaacgccag
     6361 gccgaagtcc gtgttcgcgt acggcaggcc gctgatcggg tcgttcaggc ccggcaccat
     6421 gtcgcccggc aggcccagcc tttcgtagcc ggcggtgctc aggaaatcga gctgcccgcc
     6481 ttccttgccg accagtacat tggagcccgc gatattcgcg ccgcccgcca ggtcgaagca
     6541 gatgaacgga attttcccgg cgccgacggt cgcgatgttg cacgggttcg tcagcagtgg
     6601 ggtcaggtca ccagagagcg cggcaaacgc ctgccgcgga ttcgaaaaaa gactcattac
     6661 gccgccgccc agggcgaagg ccgatcctgt caggaatccc tgcgcgacga actcacgccg
     6721 ggtcaccgga cgtcgatggt ctccatgaaa ctgcggcgca tccgggtgcc ggcttttgcg
     6781 attcctggac atattcctgc ctcgtatcct tgaagtcttc agttactgga ccagcgtgat
     6841 cgcactgccg agaacggcgg cgcagacgcc cttcgcgact gagcgggtat ccgccgtgcc
     6901 gcccatgaca aggcggtcga cgaggtttgc gggacggctt ccattcgccg tgacgtatcc
     6961 cagttcatcc cggaccacgg tgaagtcggg ttgcgtctgg atccccaatc ccatcatgtt
     7021 gtcgatcaga gggtcgataa ccaggtcacg atcggctccc gcgaacgccg ttgccggtgc
     7081 ctggttgaag ttgaaccccg ggaagtacgc ggccgacttc gccgggtcgt ccacgagcgc
     7141 atcgcaatat tgaatggcaa gttgcgcgat cgcgacctgg tgcgacgcga ggaacgcttc
     7201 gggactctcg atcgtcggca gggactggcg caggctttgg taggtcatgt cgatgtctac
     7261 ctgcttcgga tccaccgtgg taaccgcggc catggtcgcg ttgatctcgt cgaaggtgcg
     7321 tacgccgaag cggggcgcta ccggcaggtc gccctgcgtg aggaccagcg gcgggtcctc
     7381 ggtacgaacg tagctttcgg accccagcag gtcgaacgtc aggaagaact ggtcatcctg
     7441 cgggccacgc tccaggggca ccaccgcccc tatgtcggcg agcggttggc ccagctcgcc
     7501 gaataatgca gcgctgagcg cctggtccat gtttgcgtat gcctggccga cctcggcctc
     7561 cgcgccgttg atgccgatcc gcaagccctg cagcgggatt ccctccggct gctgggcgga
     7621 gtcgagcgtt atgaagtgcg gtttcgtgaa gaggtagcta tagctgtcat actgcgcgac
     7681 ttcgaaaagg atgtaagctg tagggacatt gatgatgtcc tcgatgctga acagcaggaa
     7741 aaacttctcg ccgacgccga catcaaaatt ctgcacgatc tgctccggcg tcaggacgcg
     7801 gttgtgaatt gcgaccaggc gaaacgttcc ctgccacagt ccctggccgt ccgcctcgtt
     7861 gccaaggaca agtgcgaacg tgtcctgcca gtcgaccagc gagccggtgg cgacggggtc
     7921 cgcctcagtc accagcacgc cgttgacgta gatcctgcgg ccttcgacaa cgtcgtaggt
     7981 ggcaacgaca tgttgcagcg acgcctgcaa aacctcttct gcggacggcg tcgataatgg
     8041 cgggtcgccg ttcaggtctg tttccgtgct gcggtgccgg aagttgtagt cgtacagtgt
     8101 ctgctgcagc gtgaagttgc gcacgttgtc accgccggaa tagctgacga tgcgtgccat
     8161 ttcctgggtt acgttcgccg gtactaccca ggcctcgatg gaatattcgc cagtagcgcg
     8221 aatcaggtcg tgcagcttcc tgctggccgc cgtcgatcct tgagccttgc cgtcattgat
     8281 cgtcacgccc cagccgccga accactcgta ggctccggac agggtcaggt tgatggccgg
     8341 ttctatgccg ctcgtatcga acaccgtgga gccctggccg gtcttgaact cccacaaggc
     8401 aatctgcgcg ccttcgtagc ggttgccacc agaggcgacc gttccgtcaa cgatgcgcag
     8461 cgccttgctg ttgaccagcg tcgggtcgag agcggtgatg ggcacggtat ccgcgaacgc
     8521 ctggatggcc gcctgcatct cggcggcagc cgcagtacag ttgtcgttcc agcagttatg
     8581 gaactcgctg ccgagccgga tgacgaagcg cgaatcggcg ggaacgttga ggtccatcct
     8641 cggtttcgct gcttcgtagg cgacggcgac gtccgcttct gcgaagtacg gctgctggga
     8701 ggtcgaggcc tgggaagagt ggcagctcgc gcaatacgtc gtcaggaccg gtccgtggac
     8761 cagggacgca aaattggcaa gggatgcact tgcatagttc ttgctcgagc cgggctctac
     8821 cggggccgga ggctggagaa tgatctgccg tccgccgctg gcggtggcgc cggcccagcc
     8881 ctctatccag gtcgtcatga tgtctgcgca tgcctggttg tcgctgagcc agcagttgtg
     8941 cgcttcgccg actcgctcga ccagtcgcga ctcagaaggc tgggacaggt taacgaccgt
     9001 gttggccgct tcgtaagcga ggttgatgtc gtcgtgccgg gcaaacagcg gcggctgccc
     9061 ccccgagtcg ctatggcagc ttccgcaacg gttggatgcc tggacgttgt cccacaggtt
     9121 tagcttgaaa gattgcacgt cctccgtcgc cggcggcgga ccggagtagg cagtcggcgg
     9181 cgtaacgccg gtaatgggat tttccgtcgt cgcggcgccg ccgccgcaac cgttgaggcc
     9241 gagcgtcgag acaagtgcca gcatcgtcag gccgttcatg atccggagtg accgcatgcc
     9301 gccagaaaga agccctgcaa attccgttgc gttcttcatg tcctgtagcc tctttactgg
     9361 cccatgcaat agacggctgc atccgcaaac gcctgcttca gctggtagcc ggacgaggtg
     9421 aacgcgctga ccatctggcc tacctgcgcg cgatcggcgg catcttccgg cggcctcagg
     9481 catacggcgc ggaacacctt cttgacctgg caactggcaa acgcggtgct gttgcccagt
     9541 tcctcaccca tcgatttcgc cccgttcccg cttccaggca aaccggggtc ccagcccagg
     9601 aaccggttct gcccggatcg ccagtagttt gtccagctgt catcgggcgt cacgaagcca
     9661 aacgggaagt tggaatcgtt gttgaaatac ttcggctgca cctgtcccgg cgtgtattcg
     9721 atgcttccgc tgtccgtgtt gaagttgtag tacgcgaacg cctgcgccat tggatccatg
     9781 ccggtgtggc aaccgatgca gttgttcagg aacaggcggc tgtcgccgcc ggggctcctg
     9841 ctcacatcct ggcgtatacg atccggcggc agcttgacgt ccatgacctg ctccaggtca
     9901 ttgcacatgt ggttgaggag cgtgaagcga aacattgcac ggttggtacc ggccacgaag
     9961 aaggcttctg cggccgcacg cgtggtcatg acgccagcgg tcgcggccgg gggcaggccg
    10021 gtgacggaag actgcggcgt ctgtaccagg ccacccatga gatcgacgcc ctgctgttcc
    10081 atttgctcat agtgagcgtt gttcgaattg gcatagggag gcaggcccag gtttccggcg
    10141 cctacgtaaa cgatatcggc cgacaggagc gtgttgaacg gcacgtcgtc tcgtatcatg
    10201 ccgataaccg ttgcggtgta gtcgttgagc gggacgaaga cgctctgttc gcggttcgtc
    10261 cagggagctg caaagttctt cagcgtgacg ttatagaagc ccgggttatc catcgcgatc
    10321 gtcgccgccg catgagcatt gttcatgctg atggcgttct ccatcgattg gagtacagcc
    10381 tcggtcggcg gcacaccggc caggcggtcg tgtattcgct tcgcctgttc gcgagggttc
    10441 gcggcggcaa cgcctgccag tacgaggctg aataacaacg cgatcagcag ttgcagcgaa
    10501 actccgtttc tcatcggcga cgtcctgtcg ttcatagcgt tctgctcgtt acggtaaggc
    10561 tgaaactgcg ctttggtttt tgttcgcgtt caagactatg catgaggatc acaacaacat
    10621 ccgtgactgc actcacactc gtatgggccc cgaaaaccgg cgactggcgc cgcttcatga
    10681 agacgtaaaa gcaagggctg tgccactgcg atttagggtg gcagctggga gccaaaatcg
    10741 cagattagtc aacaggttac tgtgtatgtg aaaatcggga gaggtgaatg aatatgtcaa
    10801 gcgcttgtcg ttgctttcgc tgcttcgcga catcgcgacg gccaaggcca tgtttgttat
    10861 gccgctcacc ctgtcgccgg cggcagacaa gtgtgtccgt tgacgacaac tttagcgtta
    10921 aagcaacaga cccggacgta acctggtcgc ggaacggtgc ctcactttgt ttttcgcttg
    10981 atccgtcgcg cgcctttatg tgatcgcgtt aacggtacac gcggtgcttc gtctccacta
    11041 ttacacataa cctgagtgcc cgatcgctgc gaccgtcgtt ttcaactcgc ggcgaagacc
    11101 cggctggtct ggaatttgca tcggctttcg ctgaaaaggc acgcgcaact tccgctttac
    11161 agagcgtgaa caagaacgtc acgaaaaagg cactcgtacc g
//
LOCUS       DASUOM010000161         4863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_748402,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000161 DASUOM010000000
VERSION     DASUOM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748402"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1366)
                     /locus_tag="VFY27_03215"
     CDS             complement(206..1366)
                     /locus_tag="VFY27_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5764548.1"
                     /translation="MFNIAGAALIACACLVVQTAAAAESPRARDLGVPFEGIAGPLNA
                     ITDVQGVEVGQVSIIEDLAGDRAVRTGVTAVLPLGEASRDTAVFAGWFSLNGNGEMTG
                     TTWVRESGFLEGPVMITNTHSVGAVHQATIEWRIEQGAADATGYWWSLPVVAETWDGH
                     LNDINGMHVKAEHAHAALESASRGPVAEGNTGGGTGMICHEFKCGTGTSSRMAETREG
                     TFTVGVLVQANYGMRDTLRIAGVPVGQHLVEDRAFASLPQPVQESGSIIVVVATDAPL
                     LPHQLERVAKRASLGIARVGGMAGNGSGDIFIAFSTSLPEEQEGKGTMARMHANDTIS
                     PLFDATVLATEEAIINALVAAKDMTGDRGHVVKAIDHDELRKVLQQYNRLAD"
     gene            1496..>4863
                     /locus_tag="VFY27_03220"
     CDS             1496..>4863
                     /locus_tag="VFY27_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5764549.1"
                     /translation="MAQPCDWQFWIDRGGTFTDIVALRPDGSLETAKLLSDNPEHYAD
                     AAAEGIRRVISRWDACGGTECAVAAVKMGTTIATNALLERRGAETALVVTKGFADSLS
                     IGYQNRPDIFALNIVKPAPLYTRVIEADERLTADGEVLTPLDECVLARNLEECLGSGI
                     DSVAICLLHGYRWPEHERRVARLARQLGFRQVSASHEVEPLIKFVSRAQTTLVEAYLT
                     PVLNQYIEKLKQALEGPAQPARLMFMQSNGGLVMADRFRGKDSILSGPAAGVVGMAQT
                     ATAAGFCRLIGFDMGGTSTDVSAFNGEYERSNDSEVAGVRLRAPMMKIHTIAAGGGSL
                     LRYCDGRYQVGPDSAGASPGPACYRRGGPLTVTDANVLLGRIPVEHFPRVFGPSADQP
                     LDSDVVQRLFQELAGVVSADRGALVSAEDVANGFLTVAVENMANAIRKITIERGEDAR
                     DFTLCCFGGAGGQHACRLADVLGITTVWLHPFAGVLSAYGMGLADLRVERQQSVEAPL
                     DDANLRKLQPLVAELTEQCSDLLAEQGVPAENRRFTSRAGVRVSGSDTIFELPLAGAR
                     AMRTAFANAYGDRFGSELGESELLVATLSVAATGVERSFEEPLLPFGKPAEPRSKQKL
                     WVAHGWRDVPVFRREELPGGTLLPGPAIVAEANGTTVIHRGWQARVNERAHLVIERMR
                     EAVAAEPGESRAALRSPDPVRLEVFNRLFMHIAEQMGTVLQNTALSVNIRERLDFSCA
                     LFDADGRLVSNAPHMPVHLGSMGESVRSVIAHKGGALREGDAVMLNSPYNGGTHLPDI
                     TVVTPWFSGTRAPLFYLASRAHHADIGGITPGSMPSTSRHIDEEGVLIDNVWLVRDGR
                     FREAEVRTLFGNARYPARNVEQNLADLKAQLAANRQGIRQLQRAVERYGLATVQAYLK
                     FVRENAAASVRRLLGTLSNGSFEYPLDSGELISVRIQVDRGREIATVDFAGTSSQSET
                     NFNAPEAVTRAAVLYVFRCLIVEDIPLNEGCLEPLDVVIPRGSMLSPVYPAAVVAGNV
                     ETSQCVTDALFGALGALAASQGTMNNLSFGNEKLQYYETIAGGAGAGPGWHGADAVQT
                     HMTNSRMTDPEVLEQRFPVLVESFAIRRGSGGAGR"
BASE COUNT          856 a         1543 c         1573 g          891 t
ORIGIN      
        1 ccaggcggaa gacgaacttg cggaggcgct gatcgccgcg atccacggcc ccatcctgcg
       61 cccggcgaga taaaaatcct cgatgctctg gttgcgcttg tgcgcccaca ggccgacacc
      121 gacgaggatg gcgttgtaca gcacaagcgt gacgaggacg accgccgtgc tgctcatcgc
      181 gtatgccgga ttcggatcat cctctctagt cagccaggcg gttgtattgc tgcagtacct
      241 tgcgcaactc atcgtggtcg atagccttga ccacgtgccc gcgatcgccc gtcatgtcct
      301 tcgcggcaac cagtgcgttg atgatcgctt cctcggttgc gagaaccgtc gcatcgaaca
      361 gcgggctgat cgtgtcgttg gcatgcatgc gcgccatcgt gccctttcct tcctgttctt
      421 cgggaagaga cgtagagaac gcgatgaaaa tgtcgccgga gccgttgcct gccattccgc
      481 caacgcgggc gatgccgaga ctcgcccgtt tcgctacccg ttcgagctgg tgcggcagga
      541 gcggcgcatc cgtcgccacg acgacgatga tcgagccgct ctcctgcact ggctggggca
      601 agctcgcgaa tgccctgtcc tccaccaggt gctgccccac tggaacacct gcgatacgca
      661 gcgtgtcgcg catcccgtag ttcgcctgca ccagtacgcc gaccgtgaac gtgccttcgc
      721 gtgtttccgc catgcgcgac gacgtgccgg tgccgcactt gaactcatgg cagatcatgc
      781 ctgtacctcc gccggtgttt ccttcagcaa cagggccgcg ggatgctgac tccagcgcgg
      841 catgcgcatg ctccgccttc acgtgcattc cgttgatgtc gttcaggtgc ccgtcccagg
      901 tttccgcgac gaccggtaac gaccaccagt aaccggtcgc gtccgcggcg ccctgctcga
      961 tgcgccactc gatcgtcgcc tggtgcacgg cgccgacgct gtgggtgttc gtgatcatca
     1021 cggggccttc gaggaagccc gactcgcgca cccatgtcgt cccggtcatc tcgccgttgc
     1081 cgttcaagga aaaccagccg gcaaacacgg ccgtgtcgcg actcgcctct ccgagcggca
     1141 acacggcggt cacgcccgtc ctgaccgcgc ggtcgccggc caggtcttcg atgatcgaga
     1201 cctgccctac ctcgacgccc tggacatcgg taatggcgtt gagcggcccg gcgatgccct
     1261 cgaaaggcac gccaaggtcc cttgcgcgcg gcgactctgc tgctgccgca gtctgtacaa
     1321 cgaggcatgc gcatgcaatc agcgccgcac cggcaatatt gaacattcgg gtcttctcca
     1381 tgtggagcgg ttttgccgca tcataacggc aaagccggac accgatgtac ctgttcgcct
     1441 ccaggcacgg aatgttaacg tgacgctcct tcctcgatga cgtatatcgt ttcacatggc
     1501 gcaaccgtgc gactggcagt tctggatcga caggggtggg acattcaccg acatcgttgc
     1561 cttgcgcccg gacggctccc tcgaaacggc caagctgctt tccgataacc cggagcatta
     1621 cgcggatgcc gcagccgagg ggatacgcag ggtcatctct cgctgggacg cctgcggcgg
     1681 cacggagtgc gccgttgccg ccgtaaaaat gggcacgacc atcgcaacca acgcgctgct
     1741 cgagcgccgc ggtgcggaaa ccgcgctggt tgtcacgaag ggtttcgccg acagcctgtc
     1801 gatcggctac cagaatcgcc ccgatatttt cgcgctgaac atcgttaaac cggcgccgct
     1861 ctatacccgc gtcattgagg cggacgaacg actgactgcc gacggcgaag tgctcacccc
     1921 gctggatgag tgcgtgctcg cccggaatct cgaagaatgc ctgggaagcg gcatcgattc
     1981 ggtagcgata tgcctgctcc atggttatcg ctggcccgaa catgagcggc gggtggcgag
     2041 gcttgcgcga caactgggtt ttcgtcaagt gtcggcctcg catgaagtcg agccgctgat
     2101 caagttcgtg agccgcgcac aaacgaccct ggtcgaggcg tatctcacgc cggtgttgaa
     2161 ccagtacatc gagaagctta agcaggcgct ggagggtccg gcgcagcctg cgcgcctgat
     2221 gttcatgcaa agcaacgggg gtctcgtgat ggcggaccgt tttcgcggca aggacagcat
     2281 cctctccggt cccgcggccg gcgttgtcgg gatggcgcaa accgcaaccg cggcgggctt
     2341 ctgccggctg atcggcttcg acatgggggg aacgtccacc gacgtttccg cgttcaatgg
     2401 cgagtacgag cgcagcaacg actcggaggt tgcgggcgtt cggctgcgcg cgcccatgat
     2461 gaaaattcac accattgctg caggaggagg gtccctgctg cgctattgcg acggccgcta
     2521 ccaggtcggt ccggactccg ccggtgcaag tcccggaccg gcatgttacc gccgcggcgg
     2581 accgctgacg gtcaccgacg caaatgtctt gctcgggcga attccggtgg aacactttcc
     2641 ccgggtgttc ggcccgtccg cggaccagcc gctggacagc gacgtggtgc aaagactgtt
     2701 tcaggagctc gcaggggtcg tgtcggcgga ccgcggcgcc ttggtttccg cagaggatgt
     2761 cgcgaatgga ttcctgacgg tagccgtgga gaacatggcc aacgccatcc ggaagatcac
     2821 gatcgagcgt ggcgaggatg cgcgcgactt cacgctgtgt tgcttcggcg gcgccggcgg
     2881 gcagcacgcc tgccgcctgg cagacgtgct cggcatcacc accgtgtggc tgcatccgtt
     2941 cgcgggcgtg ctgtctgctt acggcatggg ccttgcggac ctgcgcgtgg aacggcagca
     3001 gtcggtggag gctccgttgg atgacgcgaa cctgcgcaaa ctccaaccgt tagttgcaga
     3061 gttgaccgag cagtgcagcg acctgcttgc cgaacagggt gtgccggcgg aaaaccgccg
     3121 cttcacgtcg cgcgccggcg ttcgtgtcag cggttccgac acgattttcg agttgccgct
     3181 cgccggggcc cgcgccatga ggactgcttt cgcgaatgct tacggggatc gcttcggcag
     3241 cgaactgggc gaaagcgagt tgctcgtggc gacgctgtcc gttgctgcaa cgggtgtcga
     3301 acgcagtttc gaggaaccgt tgttgccgtt cggaaagccg gccgagccgc gcagcaagca
     3361 gaagctgtgg gttgcacacg gatggcggga cgtgccggtg ttccggcgcg aggagcttcc
     3421 gggaggcacg ctgttgccgg gacccgctat cgttgccgaa gcgaacggaa ccaccgtcat
     3481 ccaccggggt tggcaagctc gcgtcaacga acgtgcacat ctcgtcatcg aacgcatgcg
     3541 cgaagcggtc gctgcagagc ccggcgaatc acgcgcagcg cttcgttcgc cggacccggt
     3601 ccggctcgaa gtattcaacc ggctgttcat gcacatcgca gaacagatgg gcaccgtcct
     3661 gcaaaacacc gcgctgtcgg tgaacatacg cgagcgcctc gatttttcct gcgcgttgtt
     3721 cgacgctgac gggcgactgg tgtcgaacgc gccgcacatg ccggtgcacc tgggatcgat
     3781 gggcgagagc gtgcgcagtg tcattgcgca caaaggcggc gcattgcggg aaggcgacgc
     3841 ggtgatgctg aactcgccat acaacggcgg cacgcatctg cccgacataa ccgtcgtcac
     3901 gccttggttt tccggaacgc gcgcgccact gttttacctg gcctcccggg cgcaccatgc
     3961 cgacatcggc ggcatcaccc caggctcgat gccctctacc agccggcata tcgacgaaga
     4021 aggcgtgctg atcgacaacg tctggctggt gcgcgacggg cgctttcgcg aagcggaagt
     4081 acggacgctc ttcggcaacg ccaggtatcc cgcgcggaac gtcgagcaga accttgcgga
     4141 cctcaaggcg caacttgcgg cgaaccggca gggcatccgc cagctgcaaa gggcagtcga
     4201 acgctacggc ttggcgacgg tgcaggcgta tctcaagttc gtccgtgaga atgccgcagc
     4261 aagcgttcga cgcctgctgg gcacgctgtc gaacggctcg ttcgagtatc cactcgattc
     4321 gggagagctc attagcgtgc gcatacaggt ggaccgcggg cgcgaaatcg cgaccgtgga
     4381 cttcgcaggc acgtcgtcgc agtcggaaac caacttcaac gctcccgagg cggtgacccg
     4441 ggcggcggtg ctgtacgtct tccgctgcct gatcgtcgag gacatcccgc tgaacgaagg
     4501 ttgcctcgaa ccgcttgacg tcgtcatacc gagggggagc atgctgagtc cggtttatcc
     4561 ggcggcggtg gtcgccggca acgtggaaac ttcgcaatgc gtcaccgatg cgttgttcgg
     4621 tgcgctcggc gcgctggcgg cgtcgcaggg aacgatgaac aacctgagct tcggcaacga
     4681 gaagttgcag tactacgaaa ccatcgcggg gggcgccggg gccggccccg gctggcatgg
     4741 tgcggatgcg gtacagacgc acatgacgaa ctcccggatg acggaccccg aagttctcga
     4801 acagagattc ccggtgcttg tcgaatcgtt cgcgattcgc cgcggctcgg gcggtgcagg
     4861 ccg
//
LOCUS       DASUOM010000162         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1111325,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000162 DASUOM010000000
VERSION     DASUOM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111325"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFY27_03225"
     CDS             complement(<1..889)
                     /locus_tag="VFY27_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX5764550.1"
                     /translation="MRRRRLTLTRQILLGLVLGVIAGALVNTYHAEWASSFRPFSQLF
                     LRLIKMIIAPLIFATLVAGIAGAGHLRQVGRIGVRAIIYFEIVTTIALIIGLAAVNIT
                     RPGDGITLPPDGGEITATPKTWDEILLHTVPESVIKAMAEGEVLQIVIFSILFGIALG
                     LIGEKGQPLVRLCEVVSETMFKFTNIVMHYAPLGVGAAMAYTVGHGGIGILYNLAWLV
                     GTLYMALIVLILGVFLPAALLFKVPVRKFVKAIREPAVIAFSTTSSEAALPRAMEVLE
                     RLGVPRRIVAFVLPLGYSFN"
     gene            complement(899..1840)
                     /gene="secF"
                     /locus_tag="VFY27_03230"
     CDS             complement(899..1840)
                     /gene="secF"
                     /locus_tag="VFY27_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5764551.1"
                     /translation="MKLIKEKTSIDFLGRTSRTIAGGVAFLMLVVSLISLTTRGLQFG
                     LDFTGGILLEITYPQAVDLDQLRGLMADAGYEEAQVQRYGADTAVLVRVPPQEDADLD
                     AFRDAITATLTADEPDAKVLRVELVGSQVGEDLREQGGTAVIFALCMIFIYVMFRFQW
                     KFAVGAIVATAHDVLVVLGFFSIFGWQFDLTVLAAVLAVIGYSLNDTVVVFDRVRENF
                     LKLRGTDTEEVMNISINETLSRTLMTGMTTLMVLFALYFIAGETLEPFSLALIIGVLV
                     GTYSSIYVASATAMWLNINANDLVPAEKEIQLIDDLP"
     gene            2324..4018
                     /locus_tag="VFY27_03235"
     CDS             2324..4018
                     /locus_tag="VFY27_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764552.1"
                     /translation="MVYDAGDGRVDAPDPRGRRLLVDTSLPVMNNVAGERKLDGAADE
                     RSSLITSLLVDRRYAAGRLSSRPRAIAVCLALLALASGAALVAERFLYVQNVLLVFLP
                     VILFAAVRYGFWMALGCSVLSIASSSYFLADPRFSFAVRDPENIWALLFFLLVSAFTS
                     TLAAQLRHRTAALQRSSRVTEELFAFSSTLAAISQTDDLLRSAARRLARTLEADVRIF
                     VPGHEGLTVRGSTCGTGNVARDELDAARWTFAHGLPSGGGAGTSQNVAGLYLPLRTGR
                     GTVGVIGLFPHERKETPASHESALLDSLANQVAINLERVQLAERMQETRILAEADKLR
                     TALLTSISHDFKTPLASILGNVSSVAQYGRLYDEATRTEMLEMAEAETVRLSRFVDNL
                     LHMTRIDAGGLHPTLETLDVSDLVGSALTRSEKLLQRHRLRTDLPADLPMVDANFVLA
                     EHVLANLLDNAAKYSPPGSEIRIAAHHADDRVVMHVQDEGPGIPEEHLEQIFQRFFRV
                     MTADHRPAGTGLGLAICRGFVEAMGGTICAANRTDAPGARFTITLPRSADTGEPNR"
     gene            4015..4710
                     /locus_tag="VFY27_03240"
     CDS             4015..4710
                     /locus_tag="VFY27_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5764553.1"
                     /translation="MSAGEIRVLLMEDDPAILRFLRNTLRIQDYDVVEATNGRDGLGL
                     VRNAKPDLVILDLGLPDMDGMEVIREVRTQSQVPILVLSSRSDERGKVMALDLGADDY
                     VCKPFGVDELIARMRTALRHRLASEGSLPMFNSGGLSVDLMRRDVTVDGVKIKLSKKE
                     YEILRELVVHAGKVITHGHLLRVAWGTETGVDVQYLRVYIRQLRQKIEAAPAQPRYIL
                     TEPGVGYRLSIID"
BASE COUNT          944 a         1472 c         1440 g          901 t
ORIGIN      
        1 gattgaagct gtagccgagc ggcagcacga aggcgacgat gcgccgcggt acgccgaggc
       61 gctcgagcac ctccatcgcc cgtggcagcg ccgcctcact cgaggttgtc gagaaggcaa
      121 taaccgccgg ctcgcgtatg gccttgacga acttgcgcac aggcaccttg aaaagcaacg
      181 ccgcgggaag gaagacgccg aggatcagca caatcagagc catgtagagc gtcccgacga
      241 gccacgcgag gttgtacagg atgccgatcc caccgtgacc gacggtatag gccatcgcag
      301 cgccgacacc gagtggtgcg tagtgcatta cgatattcgt gaacttgaac atcgtctccg
      361 atacgacttc gcagaggcgc acgaggggct ggcccttctc cccgatcaga ccaagtgcga
      421 tcccgaagag aatgctgaag atgacaatct gcagcacttc gccctcggcc atggccttta
      481 tcaccgactc ggggacggtg tggagcagaa tttcgtccca ggttttcggc gtcgccgtta
      541 tctccccgcc atctggcgga agtgtgatgc cgtcgccggg gcgcgtgatg ttgacggcgg
      601 ccaagccgat gatcagagct atcgtcgtga cgatctcgaa atagataatc gcgcgcacgc
      661 cgattctgcc gacctgccgc aagtggcctg cacccgcgat gcccgcgacc agcgttgcga
      721 agatcagtgg cgcgatgatc atcttgatca ggcgcaggaa aagctggctg aacggccgga
      781 aggagctcgc ccactcggcg tgataggtat tgacaagcgc cccggcgatg acgcccagga
      841 ccagcccgag aaggatctgc cgggtgagag tcaggcggcg ccggcggatg cggaatcctc
      901 agggcagatc atctatcagc tggatttcct tttccgcagg taccaggtcg ttggcattga
      961 tgttcagcca catcgccgtc gcgcttgcga cgtaaatcga ggagtacgtg ccgaccagca
     1021 cgccgatgat gagcgccagc gagaatggct cgagtgtctc gcccgcaatg aaataaaggg
     1081 cgaacagaac catcagcgtg gtcataccgg tcatcagcgt ccgcgacaat gtttcattga
     1141 tcgatatgtt catcacctct tcggtatccg tgccgcgcag cttgaggaag ttctcgcgca
     1201 cccggtcgaa gacgaccaca gtatcgttga gggagtaccc gatcaccgcc agcaccgcag
     1261 caaggacggt gaggtcgaac tgccagccga agatcgaaaa gaaacccagt accacgagaa
     1321 cgtcgtgcgc cgttgccacg attgccccga cggcgaactt ccactggaag cggaacatga
     1381 cgtagatgaa gatcatgcaa agggcaaaga tgacggcggt gccgccctgc tcgcgcaagt
     1441 cctcaccgac ctgggagccc accagttcca cgcgcaagac ctttgcgtcc ggctcatctg
     1501 ccgtcaaggt cgcagtgatg gcgtccctga acgcgtccag atccgcatcc tcctgcggcg
     1561 gcacgcgcac gagcacggcc gtgtcggctc cgtaacgctg cacctgcgct tcttcgtaac
     1621 cggcatcggc catcagcccc cgaagctggt ccaggtcgac cgcctgggga tacgtgatct
     1681 cgaggagaat accaccggtg aaatcaaggc cgaactggag cccccgagtg gtcaacgaga
     1741 tgagcgaaac cacgagcatc aggaatgcga ccccgccagc gattgttcgc gaggtccggc
     1801 ccaggaaatc gatcgacgtc ttttctttga tcagcttcat gaacgaggtc cggccgcagg
     1861 acacctttcc tgggcccgac gaggcggaat ttccacgggg gcgccgccct gccgcgcctg
     1921 gtccgccggc gcagtgtcag ctggtcccga tcaccatcca accgccggcc attaccgcca
     1981 aggcaaacga caccagcagg atctggctca ggatccatag cgccttgttc tttctgatat
     2041 ccatcccttt tacctgtatc gatggccgtg gccgatttgt ggttggcctg actgtgcgcg
     2101 ccgcaggtat aggaattcaa ttggcttgcg tacgcctgtt catatgaaat gcataaggac
     2161 ggggatggcc cctccacgcg aacttcgtat atgcgtttcc tatgcgcctc tggctgcagg
     2221 agcattgatt tcctaggcgc cgccccgaca atctgtttct ttcttcaagc tatgcgctgg
     2281 cctatcggcg cgacgagagg ggggaagcgt tagcgctgca gcgatggtgt atgatgccgg
     2341 cgacgggcgc gttgacgccc ccgatccccg gggacgacgg ttgctcgtcg acaccagcct
     2401 tccggtcatg aacaacgttg cgggggagcg aaagcttgac ggcgccgcag acgaacgttc
     2461 gtcgctcatt accagcctgc tcgtcgaccg acgctacgcg gctggacgcc tgagtagccg
     2521 cccccgcgcg atcgccgtat gcctggcact tcttgcactc gcctcgggtg ccgcgcttgt
     2581 agccgaacgc tttctttacg tgcagaacgt tctgcttgta tttctcccgg tgatcctttt
     2641 cgctgctgtg cgctacggct tctggatggc gctgggctgt tcggtgctga gcatcgccag
     2701 cagcagctat tttctcgccg atccgcggtt ctccttcgcc gtaagggatc ccgagaacat
     2761 ctgggcgttg ctgttcttcc tgctcgtctc cgcttttacg agcacacttg cagcgcagct
     2821 gcggcatcgc acagcggcac tgcagcgaag cagccgggta acggaggaac tctttgcctt
     2881 cagctcgacg ctggcggcga tatcccaaac tgacgacctg ctgcgttctg cggcccggag
     2941 gctcgcacgg acactcgaag cggatgtgcg gattttcgtt ccggggcatg agggtctcac
     3001 ggttcgcggc agcacatgcg gcacaggaaa cgtcgcgcgc gatgaactcg atgcggcgcg
     3061 ctggaccttc gcgcatggct tgccgtccgg tggcggcgcc ggcacgtcac aaaacgtcgc
     3121 gggtctctat ctgcctctcc gcacgggccg cggcaccgtc ggcgtcatcg gcctcttccc
     3181 tcacgagcgg aaggagacac cagcatcgca tgaatcagcg ctactggact ccctggccaa
     3241 ccaggtcgcg ataaacctgg aacgagtgca gctcgcggaa agaatgcagg aaacccggat
     3301 actggccgag gcagacaagc ttcgcacggc tttgcttact tccatatcac atgacttcaa
     3361 aactccgctg gcttccatcc tcggcaatgt cagcagcgtg gcccagtatg gcaggctgta
     3421 cgatgaggca acgcgcaccg aaatgcttga gatggcagag gctgaaacgg tacggctgag
     3481 ccgctttgtc gacaatctgc tgcacatgac ccgaattgac gcgggagggc tgcacccgac
     3541 tctcgagacc ctggatgtca gcgacctcgt cggctccgcg ctcacccgat cggagaagct
     3601 tttgcaacgc catcgactcc gcacggatct tcccgcggac ctgccgatgg ttgatgccaa
     3661 tttcgttctc gcagagcacg tcctggcgaa cctcctggat aacgcggcga agtattctcc
     3721 tcccgggtcg gagatccgca tcgcggccca ccacgccgac gatcgcgtgg tcatgcacgt
     3781 gcaggatgaa ggacctggca tcccggaaga acatcttgag cagatctttc aacgtttctt
     3841 ccgtgtcatg acggccgacc atcggcccgc gggaaccggg ctgggccttg ccatctgcag
     3901 ggggttcgta gaagcgatgg gcgggacgat ctgcgctgcc aaccgcaccg acgcgcctgg
     3961 agccagattc acgatcactt tgccccgctc ggccgatacc ggggagccga accgatgagt
     4021 gctggcgaga ttcgggtcct gctcatggag gacgatcccg cgattcttcg tttcctccga
     4081 aacacgttaa ggatccagga ctacgacgtc gtggaggcga cgaacgggcg ggacgggctg
     4141 ggcctggtca ggaatgcaaa accggacctg gtcattctcg acctgggact gccggacatg
     4201 gacggtatgg aggtaatccg cgaggtccgt actcaatcgc aggtgccgat cctcgtgctg
     4261 tccagccgca gcgacgagcg cggcaaggtt atggcgctgg atctcggagc cgacgactac
     4321 gtctgcaaac ctttcggcgt ggacgaactg attgcccgca tgcgtactgc actcaggcat
     4381 cgcctggcca gtgaggggag cttacccatg ttcaacagtg gcgggctcag cgtcgacctt
     4441 atgcgcagag atgtcaccgt cgacggcgtc aaaataaagc tctcgaagaa ggagtacgag
     4501 atcctgcgtg agctggtggt gcacgcgggc aaggtgatta cgcacggaca cttactccgc
     4561 gtcgcatggg gcacggagac cggtgtcgac gtgcagtacc tgcgcgtcta cattcgccaa
     4621 ctccgccaga agatcgaggc cgctcctgcc caaccacgat acattctgac ggagcccggc
     4681 gttggctacc ggctgtcgat catcgactga gccactcatc tcacatgcgt ttcgtattcg
     4741 atgggcctgt ccaggag
//
LOCUS       DASUOM010000163        10679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1368206,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000163 DASUOM010000000
VERSION     DASUOM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368206"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2488)
                     /locus_tag="VFY27_03245"
     CDS             complement(<1..2488)
                     /locus_tag="VFY27_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5764554.1"
                     /translation="MRPTITRFFFLLFFAAFAIHAQDSVPAQEPVVQPPGPAGQGPDR
                     DARGRLPELPPDQRPLPPAAQPVPLEIPVLHEDEPVWSGTLERIATGVVSIRVDSTRA
                     FDTEWNSSSQATGFVVDAERGIILTNRHVVTPGPVVAEAIFLNNEEVELKPVYRDPVH
                     DFGFFRYDPAELQYIEPAELPLAPGAAGIGREIRVIGNDAGEQLSILAGTIARLDRRA
                     PEYGRGKYNDFNTFYLQAASGTSGGSSGSPVIDISGQVVALNAGANNTAASSFFLPLD
                     RVEPVLELLQQGKPVPRGTLQTVFLQKPYDELRRLGLQPETEAEARAAGPKGTGMLTV
                     QEVIPTSPADDELQAGDILLRVDGQLITTFVPLAEILDQKVGESVTVEVERGGSRVIT
                     KLPVTDLHEISPHEYLEFGDAVVNNLSFQQARHYNRPVSGVYVANAGYMLSRSAIPRG
                     AVISEVGGAPVTDIHDLEQELGKLADGDRTTVRYTTMEDPENSVVRSIQMDRKWFPAR
                     YCFRNDITGAWPCRDMTPGPSSADVDAGSTEFPQFGIKRIDAIAPSLVMVNFDLPYAV
                     SGVADRHYYGTGLIVDEERGFVLVDRNTVPVSMGDVSITFAGSLQVKGTVKYVHPLHN
                     LAIVAYDPANIGETPVQAANFAPKPLQTGDRVWAVGLKSDHQLVHQESTVASIDPLVL
                     PLSRTLRFRDANLEIISLVNAPSDIDGVLIDDRGQVAATWSSFAFEAGGDAAQFNGGM
                     SSDLIRELVDVARSERPIYSLEAEFGYAPLFAARKLGLGQEWLDRLEDHDPRGRRALS
                     INRLVAGTPAAGFLRNGDMLLAIDGEVVTSF"
     gene            complement(2678..4153)
                     /gene="gcvPB"
                     /locus_tag="VFY27_03250"
     CDS             complement(2678..4153)
                     /gene="gcvPB"
                     /locus_tag="VFY27_03250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5764555.1"
                     /translation="MYEPLIFEKSRQGRRAYAQAPAERIASKIPREFQREEAPMLPEA
                     SELQVVRHFTRLSQQNFSIDTHFYPLGSCTMKYNPRACNSLAMLPGFINRHPLGPDRY
                     GQGFLACMYELQEMLKAVTGMKGVSLTPMAGAQGELAGVAMIRAYHVARGEPHRLEVI
                     VPDAAHGTNPATATMCGCVVRELPTGADGDIDLDALRQAVGPNTAGIMLTNPSTLGVF
                     ERNISEVAAIVHAAGGLLYYDGANLNAILGKVRPGDMEFDVIHMNLHKTFSTPHGGGG
                     PGSGAVGVGRRLLPFLPIPIVGQHVEKAKPSYYWLAEDDVPQSIGRLSGFMGNAGVLL
                     RAYVYMRMLGRDGMRRIAEFATLNANYLMARLEQGGFELAFPGRRASHEFIVTLKRQS
                     RTLNLTAMDIAKALLDHGFHAPTTYFPLLVPECLLIEPTETETREMMDRFADAMIAIV
                     REAETDIGRVKGAPYSMPVRRLDDVKAARNLDLAWQPARNA"
     gene            complement(4182..5549)
                     /gene="gcvPA"
                     /locus_tag="VFY27_03255"
     CDS             complement(4182..5549)
                     /gene="gcvPA"
                     /locus_tag="VFY27_03255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5764556.1"
                     /translation="MPFIPHTDRDTEEMLATIGVPDIDSLFDEIPEELLIKDLDGIPD
                     AMNEMEIGRLMRARAAQDAGPSCFIGAGAYEHHIPAVVWDIATRGEFYSAYTPYQAEA
                     SQGTLQTIYEYQSMIASLTGLDASNASLYDGASALAEGALMAVRANRSTKSRHVLLAA
                     GVNDIYRKVAISIAGSQGLTFERMPLDHRTGTTDFGELRKNGDPPVAVVIQQPGFFGT
                     LEQVHEMTDLAHRQGALVIAVVNPTSLAVLTPPGRWGAEGADIACGEGQPLGVPLSSG
                     GPYFGFMSCKKALLRQMPGRIVGRTSDLDGNPGYTLTLQAREQHIRRSKATSNICTNQ
                     GLMVTAATIYLSLLGYEGLARVALASCARTAELQEMLCSIDGVDRLFEGPGFHECALR
                     LDRPVRPVLEALAERGILGGIDLSGSHPELEHALLTCATETKTREDLERYRDALRDIM
                     SVGAR"
     gene            complement(5588..5980)
                     /gene="gcvH"
                     /locus_tag="VFY27_03260"
     CDS             complement(5588..5980)
                     /gene="gcvH"
                     /locus_tag="VFY27_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5764557.1"
                     /translation="MSELPGDLRFTSEHEWLRKEEDGSVTIGITDHAQSALGDLVFVE
                     LPETGQELEAGGEMAVVESVKAASDVYAPVSGTVLEVNTALADDPEKINADPYGEGWI
                     VRMQPSDDDMGDTLDPDAYQELLDELES"
     gene            complement(6046..7146)
                     /gene="gcvT"
                     /locus_tag="VFY27_03265"
     CDS             complement(6046..7146)
                     /gene="gcvT"
                     /locus_tag="VFY27_03265"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5764558.1"
                     /translation="MGLKTPLYAKHVEAGGRMVDFGGWDMPLHYGSQIEEHHSVRRDV
                     GIFDVSHMTIVDVSDAESGQALEFLRILLANDTAKLREPGSALYSCMLNVAGGIVDDL
                     IVYFLRTSRYRLVVNAATREKDIAWIEKHAAPFPVTVAERPDLAMIAVQGPRAREAAT
                     ASFNPAWRDRALALKPFAALEVEDFFIARTGYTGEDGFEIVLPADAAGIFWQDLLDHG
                     AVPCGLGARDTLRLEAGMNLYGSDMDETTSPLESGLGWTVALESPHRNFIGRQALEQM
                     RAEPLRQRFAGLLLQDRGVLRNHQRVVVEGLHDGEITSGGFSPTLERSIALARLPAGD
                     YGRARVDVRGRLLDVRVVKPPFVRKGRAVIDL"
     regulatory      complement(7360..7468)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            7568..9358
                     /locus_tag="VFY27_03270"
     CDS             7568..9358
                     /locus_tag="VFY27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5764559.1"
                     /translation="MPAMFNFVIISVALLLSGGTANADASQEFGELLDDAWEWQLREN
                     PVFASQLGDRRYNDQWTDMSIEAIERRQQEQRQFLERLLSIDSSALDETGRMNYDLFR
                     RDLQNDINGNRFRSYLMPVSHRGGVQTLEATAEGLRLQSSRDFEDWLARMDSVDEVIE
                     QTMELQERGRRAGYMPPKTLMERVPPQISTQLVENAEDSPFYAAFRDMPEGISPDDQE
                     RLRKAARTIIEETIVPAYRDYSRYFEETYLPASRDSIGASELPYGQEFYEHRTRVYTT
                     TNLTPDEIHNLGLSEVKRIRTRMQMIIDELEFDGDFADFLVFLRTDPRFYYETPEELF
                     DAYLATAKRIDPQLVRLFGKLPRMPYGLRPIPENIAPDTTTAYYSRPAADGSRAGYYY
                     VNLYRPEVRPKYEIEVLTVHEAVPGHHLQIALSQELEDVPEFRKYSGFTAFIEGWGLY
                     SESLGGELGLYKDPYSKFGALTYEMWRAVRLVVDTGIHQKGWTRQQAIDFFKANAAKT
                     ELDIVNEIDRYIGNPGQALAYKIGQLKILELREEAEQALGEDFDIRDFHDQLLGGGAL
                     PLEVLEIRMKDWLAQQMQAGSSMTRRQDNG"
     gene            9351..9764
                     /locus_tag="VFY27_03275"
     CDS             9351..9764
                     /locus_tag="VFY27_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5764560.1"
                     /translation="MAEMTGKCLCGAVTFTAENVETEFETCHCGMCRRWTGSPMFAAS
                     VQRVRFEGEEHLKTFDSSEWASRGFCSKCGSSLFYYLKPADRYVMAVGAFDDASAFRL
                     VGEIYIDHKPPGYEFAGDLVRQTEEEVLSKFQLPG"
     gene            complement(9774..>10679)
                     /locus_tag="VFY27_03280"
     CDS             complement(9774..>10679)
                     /locus_tag="VFY27_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HEX5764561.1"
                     /translation="RLNPRRFYPRVDDKVLTKKLAIEAGMPVPELYGVISNQAEVRRF
                     SDIVADRDAFVVKPARGSGGDGIIVITGRSKRKRDNFRFASGLLVSAADIEHHISNIV
                     GGQYSLSGNPDTALIEYCVHFDPLFADVSYQGVPDIRVIVYRGYPVMSMVRLPTRTSD
                     GKANLHQGAVGAGIDLSTGRTLRGVLGNDVVDEHPDTGALISGLYIPKWDYILQSSAR
                     SSEVTGLGYVGVDMVIDKDLGPLILEMNARPGLNIQIANGCGLAHRVRRIDEIFDPAA
                     DPVARARISRQEFSAERQGSLALSD"
BASE COUNT         1965 a         3337 c         3339 g         2038 t
ORIGIN      
        1 ggaaagacgt cacgacctcg ccgtcgatcg ccagcaacat gtcaccgttg cgcaggaagc
       61 cggcagcagg cgtgccggca accaggcggt tgatgctcag ggcgcgccgg ccccgcggat
      121 catgatcttc gagcctgtcg agccactcct ggccgagccc cagtttccgc gccgcgaaca
      181 ggggcgcata accgaactcc gcttcgagtg aatagatcgg gcgctccgaa cgcgccacgt
      241 ccaccagttc gcgaatgaga tcgctgctca tgccgccgtt gaattgcgct gcgtcgcctc
      301 ccgcctcgaa ggcgaagctg gaccaggtag cggctacctg cccgcgatcg tcgatcagca
      361 cgccgtcgat gtcggatggc gcgttgacca gggagatgat ctcgagattc gcatcacgga
      421 agcgcagcgt gcgtgagagc ggcagcacca ggggatcgat cgaggccacc gtgctttcct
      481 ggtgtacgag ttgatgatcg ctcttcaggc cgaccgccca gacacggtcg ccggtctgta
      541 acggtttagg ggcaaagttc gcggcctgaa cgggtgtctc gccgatgttc gcgggatcgt
      601 aggcgacgat cgcgagattg tgcagcgggt gcacgtactt caccgtgccc ttcacctgca
      661 acgaacccgc aaaggtgatg ctgacatcac ccatcgacac ggggaccgtg ttcctgtcga
      721 ccagaacgaa gccgcgttcc tcgtcgacga tcaggccggt tccgtagtag tggcggtcgg
      781 cgacgccaga aaccgcgtac ggcaggtcga agttgaccat caccaatgac ggagcgatgg
      841 cgtctatccg tttgataccg aactgcggaa actcggtgct gccggcgtcg acatccgcgc
      901 tggacggtcc cggagtcatg tcgcggcagg gccaggcgcc ggttatgtcg ttgcggaagc
      961 agtaccgggc cggaaaccat ttccggtcca tctggatgga tcggacgacg ctgttctccg
     1021 ggtcctccat cgtcgtatag cgcaccgtgg tgcgatctcc atcggccagc ttgccgagtt
     1081 cctgctcgag gtcgtgtatg tccgtgacgg gtgcgccgcc gacttcggag attaccgcgc
     1141 cgcgcggaat cgccgagcgc gaaagcatgt aacccgcgtt ggccacgtac acgccgctga
     1201 cgggccggtt gtaatgccgc gcctgctgga acgacaggtt gttgacgacg gcgtcgccga
     1261 actccaggta ctcatgcggg gagatttcgt gcaggtccgt gacggggagt ttcgtgatca
     1321 cgcgcgaccc gccgcgctcg acctcgacgg ttacgctttc gccgactttc tggtcgagga
     1381 tctcggcgag cggcacaaat gtcgtgatca gctgaccgtc gacccgcagc aggatatcgc
     1441 ccgcctgcag ctcgtcgtcg gcaggcgacg tcgggattac ctcctgtacc gtcagcatgc
     1501 ccgtaccctt gggacccgcc gcgcgcgcct ctgcttccgt ctccggttgc aggccgagcc
     1561 gcctcaactc gtcgtacggt ttctgcagga ataccgtttg cagcgtgccg cgcggaaccg
     1621 gcttgccctg ctgcaacagt tccagtaccg gttcaacgcg gtccagcggc aggaagaaac
     1681 tgctggccgc cgtgttgttg gcgcccgcat tgagcgcgac aacctggccc gagatgtcga
     1741 tcaccggcga cccggacgat cctcccgagg ttccggaggc cgcctgcagg taaaaagtat
     1801 tgaagtcgtt gtacttgccc cggccatact ccggcgcacg gcgatcgagg cgcgctatcg
     1861 tgccggcaag aatggagagc tgctctcccg cgtcattgcc gatgacccgg atttcgcggc
     1921 cgataccggc ggcaccggga gcaagcggca actcggccgg ttcgatgtac tgcagctcag
     1981 cgggatcgta gcgaaagaat ccaaaatcgt gcacggggtc ccggtagaca ggcttcagct
     2041 cgacttcctc gttgttcagg aagatggctt cggcaaccac cgggcctggc gtcacgacgt
     2101 gacggttcgt gaggataatg ccccgctcgg catcgactac gaacccggtg gcctggctgg
     2161 aggaattcca ttccgtgtcg aacgcccgcg tgctgtcgac gcgaatggac acgacacctg
     2221 ttgcgatccg ttcgagggtt cccgaccaca ccggttcgtc ttcgtgaagg acaggtatct
     2281 cgagcggcac cggctgagcg gccggcggca gcggccgctg gtcgggtggc agctccggca
     2341 gcctgccccg ggcgtcgcgg tccgggccct ggcccgcggg accgggcggc tgtaccaccg
     2401 gctcctgggc aggcacggaa tcctgggcat gaatcgcgaa ggctgcaaaa aacagcagga
     2461 aaaaaaatcg ggtgattgtt ggtcgcatgt aacccttggg tccgtgagtc aagtggcgtc
     2521 cctgcagtcg tcggtggatg tgtcgttcac tggactagca gaatacgccg cggttccctg
     2581 ccgggtctgt ggcgatcctc gcagtattgg aacgtgcagg agcctggccc ttacgcttca
     2641 acatcttaca cgacggtcac cggcgcagcc tcctgcttca ggcgttgcgc gcaggctgcc
     2701 aggcaagatc caggttgcgc gccgccttga cgtcatccag ccgccgcacc ggcatgctgt
     2761 aaggtgcccc cttcacccgg ccgatgtcgg tttcggcttc cctgacgatg gcaatcatcg
     2821 cgtccgcgaa gcggtccatc atttcgcgcg tctccgtttc cgtcggttcg atcagcaggc
     2881 actccggcac cagcaacggg aagtaggtag taggggcgtg aaagccgtga tccagcagcg
     2941 ccttcgcgat gtccatggcc gtcaggttca gcgttcgcga ctggcgcttc agcgtcacga
     3001 tgaattcatg gctcgcgcgg cggccgggga acgcgagttc gaagccgccc tgttcgaggc
     3061 gcgccatcag gtagttggca ttgagcgttg caaattccgc gatgcggcgc atgccgtcgc
     3121 gtcccagcat gcgcatatat acgtaggcgc gcagcaggac gcccgcgttg cccatgaaac
     3181 ccgagaggcg gcctatgctt tgcggcacgt cgtcttcggc gagccagtag tacgacggct
     3241 ttgccttttc aacgtgctgg cccacgatcg gaatcggcag gaacggcagc aatcgccgcc
     3301 cgacgccgac ggctccggag ccgggacctc cgccgccatg gggcgtggag aaggtcttgt
     3361 gcaggttcat gtggatgacg tcgaattcca tgtccccagg ccgcaccttg ccgagaatgg
     3421 cgttcaggtt ggcgccgtcg taatagagaa gtccgcccgc ggcatgtacg atcgccgcga
     3481 cttcgctgat gttgcgctcg aacacgccca gggtggaggg gttcgtcagc atgatgccgg
     3541 ccgtgttcgg ccccaccgcc tggcgcagcg cgtcgaggtc tatatcgccg tcagcgccgg
     3601 ttggcaattc gcgaaccacg cagccgcaca tcgtggccgt tgcgggattg gtaccgtgag
     3661 cagcgtccgg cacgatcacc tccaggcgat gcggctcgcc cctggccaca tgataggcgc
     3721 ggatcattgc aacgcccgcc agttcaccct gtgcacccgc catcggcgtc agggacacgc
     3781 ccttcatgcc ggtgacggct ttcagcattt cctgcagttc gtacatgcac gcgaggaagc
     3841 cctgcccata ccggtcgggg ccgagcgggt gccggttaat gaatccgggc agcatggcaa
     3901 gcgagttaca cgccctcggg ttgtacttca tcgtgcacga cccgagcgga tagaaatgcg
     3961 tatcgatcga aaagttctgc tgcgaaagcc gcgtaaagtg ccggacgacc tgcaattcgg
     4021 aggcttcagg caacatcggc gcctcctctc tctggaattc cctcgggatt ttgcttgcga
     4081 tgcgctctgc cggcgcctgc gcgtacgcgc ggcggccttg cctggatttt tcgaatatca
     4141 gcggttcgta catgttgttg tttcctcggc tcctggcagg ctcatctcgc gccgacactc
     4201 atgatgtcgc gcagcgcgtc gcggtagcgt tccaggtctt ccctggtctt ggtttcggtg
     4261 gcgcaggtca gcagcgcatg ttcaagctcc gggtgtgaac cggaaagatc gatgccgccc
     4321 aggatgcctc gttcggcgag cgcttcgagc accggtcgca cgggccggtc gaggcgcagg
     4381 gcgcactcgt ggaagcctgg gccctcgaac aggcgatcga ctccgtcgat tgagcacagc
     4441 atctcctgaa gctctgccgt gcgcgcgcag gaagccaggg cgacgcgagc caggccttcg
     4501 tatcccagga gtgacaggta aatggtcgcc gccgtgacca tcagtccctg gttcgtgcag
     4561 atgttggaag tcgctttcga gcggcggatg tgttgttcgc gcgcctgcag ggtgagggtg
     4621 tacccgggat tgccgtcgag atcgctggtt cgcccgacaa ttcgccccgg catctgtcgc
     4681 agcagcgcct tcttacagga catgaagccg aaatagggac cgccggagga cagcggcacg
     4741 ccgagcggct gtccttcgcc gcaggcgatg tccgcgccct ccgctcccca ccttcccggg
     4801 ggcgtgagga ccgcaagcga tgtcggattc acgacggcaa tgaccaacgc gccctgcctg
     4861 tgcgcgagat cggtcatttc atgtacctgc tcgagcgtgc cgaaaaagcc aggctgctgg
     4921 atgaccaccg caaccggcgg atcgccgttc ttccgcagct ctccgaaatc ggtcgtgccg
     4981 gtacggtgat ccagcggcat acgttcgaag gtgaggccct ggctgccggc gattgaaatc
     5041 gcaaccttcc tgtagatgtc gtttaccccg gctgccagca ggacgtgcct cgacttcgtg
     5101 cttcggttgg cgcgcacagc catcagcgcg ccctctgcaa gcgccgacgc gccgtcataa
     5161 agcgatgcgt tgctggcatc gagcccggtc agtgaagcaa tcatcgactg gtattcgtag
     5221 atcgtctgca gcgtgccctg gctcgcctcc gcctggtaag gggtgtacgc gctgtaaaat
     5281 tcgccgcgtg tcgcaatgtc ccacaccacc gccggaatgt gatgctcgta tgcacccgcg
     5341 ccgatgaagc aggatggtcc ggcgtcctgc gcggcgcgcg cgcgcatgag tcgaccgatt
     5401 tccatctcgt tcatcgcgtc cgggatgccg tcaaggtcct ttatcagcag ctcttccggg
     5461 atttcatcga agaggctgtc gatgtccggc acaccgatgg tggccagcat ctcttcggtg
     5521 tctctgtccg tatgcggtat gaaaggcatt gttcagcttc gcctggtgct tttttgagat
     5581 gagcgattca tgattcgagt tcgtccagca gttcctggta ggcgtcggga tccagggtat
     5641 cgcccatgtc gtcatccgaa ggctgcatcc ggacgatcca gccttcgccg tacgggtctg
     5701 cattgatctt ctcggggtcg tccgcaagcg cggtgtttac ttccagcacc gtgcccgaga
     5761 ccggcgcata aacgtcggag gcggccttta cggattcgac gaccgccatc tcaccgcccg
     5821 cttccagttc ctggcccgtc tccggcaatt cgacgaagac gagatcaccg agcgcggact
     5881 gtgcgtgatc ggtaatacct atcgtgacgc tgccatcctc ttccttgcgc agccattcat
     5941 gttcgctggt aaagcggaga tcgcccggca attcgctcat ttgtttcact cccgttgtag
     6001 gacatgttgg aacgaccaca ggtaaagagg gatgggtgct ctcgatcaaa gatcgatgac
     6061 tgccctgccc ttgcggacga agggcggctt cactacgcgc acgtcgagta gtctcccgcg
     6121 cacatccacc ctggcgcgcc cgtaatcgcc ggccggcaac ctcgccaggg cgatcgagcg
     6181 ctcaagtgtt ggtgaaaacc caccgctggt tatttcgccg tcgtgcaggc cttccacgac
     6241 gacgcgctgg tgattgcgga gcacgccgcg gtcttggagc aacagcccgg cgaagcgctg
     6301 ccgcagcggt tcggcccgca tctgctccag tgcctgccgc ccgatgaagt tgcgatgggg
     6361 cgactcgagc gccactgtcc agccaagccc ggattccagc ggcgatgtgg tttcgtccat
     6421 gtccgatccg tacaggttca tgccggcttc cagtcgcagc gtgtcgcgag cgcccaggcc
     6481 acatggcacg gccccgtggt ccagcaggtc ctgccagaaa attcccgcgg cgtcggcggg
     6541 caggactatt tcaaagccgt cttcgccagt gtacccggtg cgggcaatga agaagtcctc
     6601 cacctccagc gcggcgaacg gtttcagggc gagcgcccgg tcgcgccagg cagggttgaa
     6661 tgaagcggtt gcggcctcgc gcgcccgtgg cccctgcacg gcgatcatcg caagatcggg
     6721 ccgttcggcc accgtgaccg ggaacggcgc tgcgtgcttc tcgatccacg cgatgtcctt
     6781 ttcccgcgtc gcggcgttca ctaccaggcg gtagcgggag gtcctgagga aatagacgat
     6841 caggtcgtct acgatcccgc ccgcaacatt cagcatgcag ctgtaaagtg cgctaccggg
     6901 ctcgcgcagt ttcgcggtgt cgttcgcgag caggatgcgc aggaattcga gtgcctgccc
     6961 gctttctgca tcggacacat cgacaatggt catgtgagac acatcgaaga tgccgacatc
     7021 gcggcgcacg gagtgatgct cctcgatctg cgaaccgtaa tgaagcggca tgtcccagcc
     7081 gccgaaatcg accatgcggc cgccggcctc gacatgcttg gcataaagcg gcgtcttcag
     7141 tcccatcgac aatatcctcg agggttggct ccactggtga gttgagcgag tccgaagtct
     7201 tgaatggctt caggcaacaa aaaagggcgg ttcgcgtgcc cgtgcttgca aaccgcccct
     7261 ctgtccaaaa tacctgagag atcaagcgcc ggagtagtcg aactcgaagc agcgcatccc
     7321 cttcggtggg ccgcaaaagg gcctctctcc agagctgatc aggattacgc ggtccctgct
     7381 gcctgagcgt ttccgagcga gttgcgcctt cggcgccccg tgcatgaccg gtaatgcgtc
     7441 aagccggagt ctcttccacg tatcctgtcg atcagtgcgc gcaataatat ccaagtccca
     7501 tcgcagttgc cagccgtcgg caacggggga acaatgcaaa gtgatcccga ctgcaggagc
     7561 ttgaacgatg ccggcaatgt ttaactttgt aattatctcc gtggccctgc tgctctcggg
     7621 tggtacagcg aatgccgatg catcgcagga gttcggcgag ctgcttgacg atgcctggga
     7681 atggcagctg cgggagaatc cggtcttcgc ctcgcagctc ggggacaggc gttacaacga
     7741 ccagtggacc gacatgagca tcgaagccat cgagcgtcgc cagcaggagc aacgccaatt
     7801 cctggaaagg ctgctatcta tcgactcttc ggcactcgat gaaaccgggc gaatgaacta
     7861 cgacctgttc cgtcgcgacc tgcagaacga catcaacgga aaccgttttc gcagctacct
     7921 gatgcctgtc agccatcgcg gcggagtgca gacactggag gccaccgccg aaggactgcg
     7981 cctgcagtcc agccgggatt tcgaagactg gctcgcgcgc atggattcag tagacgaggt
     8041 tatcgaacag accatggaac tgcaggagcg gggacgacgg gcagggtaca tgcccccgaa
     8101 gaccctgatg gagcgggtac cgccgcagat ttccacgcaa ctggtggaga atgcagaaga
     8161 cagccctttc tatgccgcgt tccgcgatat gccggagggc atctcaccgg acgaccagga
     8221 gcgcctgcgc aaggctgcga ggacgatcat cgaggaaacg atcgtgccgg cgtaccgtga
     8281 ctacagccgt tacttcgaag agacctacct tcccgcgagc cgcgacagca tcggcgcctc
     8341 tgaactgccc tacggccagg agttctacga gcaccgcacg cgcgtctata ccaccacgaa
     8401 cctgactccg gacgagattc acaaccttgg cctctccgaa gtcaagcgta tccggacccg
     8461 gatgcaaatg atcatcgatg aactcgagtt cgatggcgat ttcgcggatt tcctcgtatt
     8521 cctgaggacg gaccctcgct tctattacga gacgccggag gaacttttcg acgcatacct
     8581 ggcaacggcg aaacgtatcg atccgcaact cgtgcggctg ttcggcaagc tgccgcgaat
     8641 gccctacggc ctgcgcccga tcccggagaa catcgctccg gacacgacca cggcttacta
     8701 cagccggccg gcggccgatg gcagtcgcgc cggctactac tatgtgaatc tctatcgtcc
     8761 ggaggtcagg ccgaagtacg aaatcgaggt actgacggtg cacgaagcgg tgccgggtca
     8821 ccacttgcag atcgcgctgt cgcaggaact ggaagacgtg cccgagttcc ggaaatactc
     8881 gggctttacc gcgttcatcg aggggtgggg tctgtacagc gaaagtctcg gcggagaact
     8941 gggactgtac aaggatcctt attcaaagtt cggcgcgctg acctacgaga tgtggcgggc
     9001 cgtgcgcctc gttgtcgaca ccgggatcca tcagaagggg tggacacgcc agcaggccat
     9061 cgatttcttc aaggcaaatg cagccaagac agagctcgac atcgtgaacg aaatcgaccg
     9121 ctacatcggc aacccggggc aggcgctggc ttacaagatc gggcagctca agatcctcga
     9181 gctgcgggag gaagcggaac aggccctcgg ggaagacttc gacatccgcg attttcacga
     9241 ccagttgctt ggtggcggtg cgctgccgct cgaggtactg gagattcgca tgaaggactg
     9301 gctggcacag cagatgcaag ctggttcttc aatgaccagg aggcaggaca atggctgaga
     9361 tgaccgggaa gtgcctgtgc ggcgccgtaa ccttcaccgc tgagaatgtc gagacggagt
     9421 tcgagacctg ccactgcggc atgtgccgcc gctggaccgg aagcccgatg ttcgccgcca
     9481 gcgtccagcg cgtcaggttc gagggcgagg aacacctcaa gaccttcgac tcgtccgagt
     9541 gggcgtcgcg tggcttctgc agcaagtgcg gctccagcct tttctattac ctgaagccgg
     9601 ccgaccgtta tgtcatggct gtcggagcgt tcgacgacgc ctccgcattc cgcctggtcg
     9661 gcgagatcta tatagaccac aagccgcccg gctacgagtt cgcgggcgac cttgtgagac
     9721 agacggaaga agaagtgctc tccaagttcc agctgccggg ctgactgtct gcctcaatcg
     9781 gagagggcga gcgacccctg tcgttccgcc gagaactcct ggcgcgatat gcgggcgcgg
     9841 gctaccgggt ccgcggccgg gtcgaagatc tcgtcgatac ggcggacgcg gtgagccagg
     9901 ccgcacccgt tggcgatctg gatgttgaga cccgggcgcg cgttcatttc gaggatcagg
     9961 ggccccaggt ccttgtctat gaccatatcg actcccacat agcccaggcc cgtgacctcg
    10021 ctgctgcgcg ccgaagactg cagtatgtag tcccacttcg ggatgtacag cccggatatg
    10081 agtgcaccgg tatcgggatg ctcgtcgacc acgtcattgc cgaggacgcc gcggagcgtt
    10141 cgcccggtgc tgaggtcgat gccggcgcca accgctccct gatgcaggtt tgccttgccg
    10201 tcggaagtgc gtgtcggcag gcgcaccatc gacatcaccg ggtatccgcg gtaaacgatc
    10261 acccggatgt ccggcacgcc ctggtaacta acatcggcaa acagcggatc gaagtgcacg
    10321 cagtactcga tcagtgccgt atccggattg ccgctgagac tgtactggcc tccgacgatg
    10381 ttcgagatgt ggtgctcgat atccgctgcc gagaccagga gcccgctcgc aaaccggaaa
    10441 ttgtcgcgtt tgcgcttgct gcggccggtt atgacgatga tgccgtcgcc gccactgccc
    10501 ctcgccggct tcacgacgaa agcatcccga tcggcgacga tatccgaaaa acgccgcacc
    10561 tctgcctggt tgctgatcac gccgtaaagt tcgggaaccg gcatgcctgc ctcgatcgcc
    10621 agcttcttcg tcagcacctt gtcgtctacc ctcggataga atcgccgcgg gttcaggcg
//
LOCUS       DASUOM010000164         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1492933,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000164 DASUOM010000000
VERSION     DASUOM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492933"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1359)
                     /locus_tag="VFY27_03285"
     CDS             complement(106..1359)
                     /locus_tag="VFY27_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764562.1"
                     /translation="MTRTASFCRFALRYVPMVALGASLLLHVPMEAEAQQWRLEPELR
                     VGGEYDDNAQLRVEEENIHEITGYILEGSAGIGYVTPRTTFEITPRLRSRVYDEEIDV
                     DSDDQFLDLDWMHETLKSEFMLRGDYSRESARTAERADADIDGEDPDEIPIDETGITF
                     FNQRRERIRVTPSWRYEFTERMSFRTGYTYTDTSYEQLAGIPLRDYTDHRVDLALARE
                     LSPRTEGYIKTTARRYESDQGRDVDGVGLSIGIESDISETTRFQAEVGYEDSSTEEQL
                     PTGTEEVSDANMTGNIYLIRRLETITMLAQYRRDMSAGGSGRVSARDTLNLSLGKQFT
                     ERVSGKLGVRAYQTEGLGEDEAVTFEERDYAQFYAGLTVALSQTMLLEADYRYERVDQ
                     TSFDATADSNNFILWLVYQPTETVW"
     gene            complement(1520..>2377)
                     /gene="pgi"
                     /locus_tag="VFY27_03290"
     CDS             complement(1520..>2377)
                     /gene="pgi"
                     /locus_tag="VFY27_03290"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5764563.1"
                     /translation="VGGRYSIWSAVGLALALVIGAENFKAMLAGARRMDQHFRHAPPA
                     ENMPILLALVAIWNNNFLGAESQAILPYDNRLDRFPAFLQQLQMESNGKSVRRDGRPV
                     KVKTGMVIWGEAGSNAQHSFYQLLHQGTRLVPCDFILPVQSSGASQSQQDLAIANCLA
                     QSEALMEGYSKARTLEDLEAAGLAAAEANALAAHKAHPGGRPSNTLVFAGLSPEVLGQ
                     LIALYEHKVFVEGVIWGINSFDQWGVELGKRLATNLAAAVAAPQAYRGGNASTRGLLQ
                     HIDLLRADD"
BASE COUNT          444 a          731 c          695 g          507 t
ORIGIN      
        1 gaaggcaagt gcagttcccg cacgtattcg gcgcgctgac cagacgggca gtggtcggcg
       61 actgggaggc ctgtacgaaa atttcgcttc agcgtcagcg gaacctcacc atacagtctc
      121 agtcggctgg tacaccagcc acagaatgaa gttgttcgaa tcggcggtag cgtcgaaact
      181 ggtctggtcg actcgctcat aacgatagtc cgcttccagc aacatcgtct gcgacagggc
      241 gaccgtcagg ccggcataga attgcgcgta gtccctttcc tcgaaggtca ccgcctcgtc
      301 ctcacccagg ccttcggtct gataggcgcg tactccaagc tttccggaaa ctctctccgt
      361 aaactgcttt ccaaggctca ggttcagcgt gtcccgcgcg gacacccggc ctgaaccgcc
      421 cgcgctcata tcacgccggt attgcgcaag catcgtaatc gtttccagcc ttcgtatgag
      481 atagatgtta cccgtcatgt tcgcatcgga gacttcttcg gtcccggtgg gaagttgttc
      541 ctcggtactc gagtcctcgt atcccacttc cgcctggaac cgcgtcgttt ccgaaatatc
      601 ggattcgatg ccgatgctga ggcccacacc atccacgtct ctcccctggt cgctctcata
      661 acggcgggca gtggtcttga tgtagccctc ggtacgagga gagagctcgc gtgccaacgc
      721 caaatcgaca cggtggtctg tgtaatcccg cagcgggatt ccggcaagct gttcgtagga
      781 agtatcggtg taggtatagc cggtccggaa ggacatccgc tccgtgaact cgtagcgcca
      841 gcttggcgtc acgcggattc tctcccgccg ctggttgaag aaggtgatgc cggtctcgtc
      901 gatcggtatc tcgtccgggt cttccccatc gatatcggca tcggcacgtt cggccgtacg
      961 agcggattcc ctgctgtagt cgcctcgcaa catgaactca cttttcagtg tttcgtgcat
     1021 ccagtcgagg tcgaggaact gatcgtccga gtccacatcg atctcctcat cgtagacccg
     1081 cgaacgcaat cttggagtga tttcgaacgt ggtccgggga gtcacgtagc cgatgccggc
     1141 actgccttcc aggatatatc cggtgatctc gtgaatgttc tcctcctcga cccggagctg
     1201 cgcattatcg tcatattcgc cgccgacgcg gagttccggc tcgagccgcc attgctgggc
     1261 ctctgcctcc atcggcacgt gcaacagcag tgaggcgccg agcgccacca tcgggacata
     1321 acggagggca aagcggcaga agcttgcggt tcttgtcatt gggaaggttc cgtaggtacg
     1381 gggtagtggc gtcaggaata cccgggttaa gtaaaattgc agttcagcag cggaacgggc
     1441 gcatgtcctg tgaacgcata actttgtcat acgagcggag ccctgtacag gggaccgcct
     1501 ccgctgtcgc agaaaagcgt cagtcgtcgg cgcgcaagag atcgatgtgc tgcagcaggc
     1561 cgcgggttga tgcgtttccc ccgcggtagg cttgcggcgc tgccacagcc gctgccaggt
     1621 ttgtcgcaag tcgctttccc agttccaccc cccactgatc gaaggagttg atgccccata
     1681 taacgccctc tacaaacacc ttgtgctcgt agagcgctat caactggccg aggacctcgg
     1741 gcgagagccc cgcgaaaacc agcgtgttgc tgggacgtcc acccgggtgc gccttgtgtg
     1801 ccgccagcgc attcgcttcc gctgcggcca gccctgccgc ttcaagatcc tcgagcgtac
     1861 gtgctttcga gtagccctcc atcagcgctt cgctttgcgc gaggcagttg gcgatcgcca
     1921 ggtcctgctg tgattgcgac gcaccggaag attgcactgg cagaatgaag tcgcagggta
     1981 cgaggcgggt gccctggtgc aacaactggt aaaaggaatg ctgggcgttc gacccggcct
     2041 cgccccagat caccatgccc gtttttacct ttaccggcct gccgtccctg cggacgctct
     2101 tgccgttgct ttccatttgc agctgctgca ggaacgcagg gaatcggtca agccggttgt
     2161 cgtacggcag gatcgcctgg ctttccgcac ccaaaaagtt gttgttccag atggcgacca
     2221 gtgccagcag gatcggcatg ttctccgccg gtggcgcgtg ccggaaatgc tggtccatcc
     2281 tgcgcgcacc tgccagcatg gccttgaagt tttcggcacc gatcacgagc gcaagcgcga
     2341 gaccgacggc ggaccagatg gaataacggc caccgac
//
LOCUS       DASUOM010000165        15856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_94747,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000165 DASUOM010000000
VERSION     DASUOM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94747"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFY27_03295"
     CDS             complement(<1..123)
                     /locus_tag="VFY27_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5764564.1"
                     /translation="MKTTIVGGGPGGLYFAILAKKAWPAWDITVYERNRPDDTFG"
     gene            complement(164..946)
                     /locus_tag="VFY27_03300"
     CDS             complement(164..946)
                     /locus_tag="VFY27_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764565.1"
                     /translation="MTDPGAPILRAARAEHWREVVVRFAHIDAAGIVFYPRYLEMLGQ
                     AFPELALAKEPFTLDIGFRKPTPLGTRLKLALTASALREGWRVSGVAGGEEQFTMLWQ
                     PADDLLSGLQDHDPARPAFRSEAMLIEDWAAGPDARLQTSRYYEIVNAAVEQWFENEL
                     MLPFSRLHRDRAGIPTVSLRTVCAELPRSGETISVWIRPTRIGRSSVHFESWLVGPGG
                     CAARTSQAIVFVRLEQDGFRSAQLPPALRSQLVRHLAETASA"
     gene            1160..1537
                     /locus_tag="VFY27_03305"
     CDS             1160..1537
                     /locus_tag="VFY27_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5764566.1"
                     /translation="MRSRFRGHFTVTLPQFDVLAELKRAGRPLTMSQLSSELMVSNGN
                     ITGVVDRLEKGGLVSRDRCNKDRRVQFIALTPLGAKQFQQMARRHEKWLAELFSELTV
                     NDMDDLQKLLLKARRSATTGTAA"
     gene            complement(1562..1963)
                     /locus_tag="VFY27_03310"
     CDS             complement(1562..1963)
                     /locus_tag="VFY27_03310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5764567.1"
                     /translation="MKHLLPEGWPRPKGYSNGILAEGQFLFVAGMIGWDEHGRFPVDF
                     VSQLAQVLKNTVAVLEAGGTGPGNIVRMTWYVKGLDLYRNNMSDVGRVYLDIIGKNFP
                     TMAVVGVSDLVEPEALIEIETTAVVGPASRK"
     gene            complement(1960..3129)
                     /locus_tag="VFY27_03315"
     CDS             complement(1960..3129)
                     /locus_tag="VFY27_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5764568.1"
                     /translation="MADRTYLDWPFLDDEHRILHARLSEWCDSYDFEHDDDTDTACRK
                     LVNDLGHAGWLRHSVPAAWGGARDALDVRSIALSREILAYHSGLADFSFAMQGLGSGA
                     IALAGNDAQREQYLPSVASGHSIAAFALSEANAGSDVAALQTSAELSGDEYVLNGEKT
                     WISNGGIADFYCVFARSGTEPGARGLSAFIVDAKAPGLRIADRIETIAPHPLATLRFE
                     DCRIPASARIGEPGKGFRIAMATLDVFRTSVAGAALGFARRALDESVSHTSHRTLFGA
                     PLSALQMTQASIAEMALEVDASALLIYRSAWAKDCYQERVTREAAMAKLYATEAAQRI
                     IDRAVQLFGGRGVTKGNIAESLYREIRALRIYEGASEIQKVIIARQTYANAEVTQ"
     gene            complement(3129..3941)
                     /locus_tag="VFY27_03320"
     CDS             complement(3129..3941)
                     /locus_tag="VFY27_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX5764569.1"
                     /translation="MTESTIKPEHFLWEVESNGVATVTLNRPERKNPLTFESYAELRD
                     TFRALVFEDSVRAVVIAGAGENFCSGGDVHEIIGPLTKMDMKGMLRFTRMTGDLVKAL
                     RQCPQPVIAAIDGVCAGAGAAIAMASDLRYGTARAKTAFLFARVGLAGADMGACAMLP
                     RIIGQGRASELLFTGRSMSGEEACRWGFFNALYEPASLLEESRSQAARLAAGPNFAHG
                     ITKNQLNIEWDMSLDTAIEAEAQAQAICMQTADFERAYRAFVARQKPEFEGN"
     gene            complement(3941..4717)
                     /locus_tag="VFY27_03325"
     CDS             complement(3941..4717)
                     /locus_tag="VFY27_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5764570.1"
                     /translation="MTYAFPSTFENRHVVVTGGAGGIGTAIAERFADAGAPVTIVGRN
                     PDKLRATAERLGIHAAMADVTVRGEVEAAISDAVAARGPIRILVNNAGSAEAAPFSKT
                     DEALWDRMLAVNLTGTYLCTHAVIGYMVAAGDGRIVNIASTASLTGYPYVSAYCAAKH
                     GVLGLTRSLALEFARKGVTVNAVCPGYTDTELVERSIDNIVASTGRNRDDAMAELLKV
                     NPQRRLVKPDEVADAVLWLCGPNAASVTGQAIAIAGGEVM"
     gene            complement(4714..6228)
                     /locus_tag="VFY27_03330"
     CDS             complement(4714..6228)
                     /locus_tag="VFY27_03330"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEX5764571.1"
                     /translation="MAATGERPITIAMLALGGQGGGVLSNWLVDLAEANGFLAQSTYV
                     AGVAQRTGATVYCVEMLRRAAAEAEGRPPVFAQYPVPGDVDIVIASELAETGRALEKG
                     FVTPNITTLIASSHRDYAISEKTALGDGIMDLQPVMEAAREAAQRFICFDMAARARET
                     SSVISAVIFGAVAGSGALPFQREQYEEVIRASGRAVGTNLAGFAAGFAGAHNHTSTTA
                     VVEQMPLAMPKGPAGKALAVRIDGMLPAGCRNMALHGALRTLSYQDQAYADLYLERLA
                     RIESLDRSQPHASANPDLTETVARELALQMCYEDTIRVAEIKTRTDRLAGIGRDIGAA
                     SGQPWYVTEYFHPRFEEMCDTLPAGLGARAVASPAARRWTAPLFRTGRNLNTNKIWGY
                     VLLSTLARLRRWRRGTLRYTVQQQHIEGWLAAVAETATVDYEGAVALAGSIEIVRGYG
                     ETYERGLKRYRTMLEASRRAAPGTKADLLRRLHGAALADEQGSAFEKTLSEING"
     gene            complement(6230..8362)
                     /locus_tag="VFY27_03335"
     CDS             complement(6230..8362)
                     /locus_tag="VFY27_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX5764572.1"
                     /translation="MAERSFKKEVEKLKIGAGEEFAGEGILAVTKALLQSGVAYVGGY
                     QGSPISHLMDVLSDAQEILGELGVHFESSASEAAAAAMAAASVNYPLRGAVTWKSTVG
                     TNVASDALANLASGGVTGGVVVIVGEDYGEGSSIMQERTHAFAMKSQMWLIDPRPNLP
                     SIVDAVEHAFELSEASHTPVFLEMRIRSCHVHGRFVAKDNQPPRMPLREAVENPRRDV
                     DRIVLPPASFLHEQEKVNERWPAAIRYIKEHGLNEVFDGERGDIGIIVQGGLYNSLIR
                     TLERLNLADIYGRTQIPLYVMNVAYPLVDDELVDFCRGKRAVLLVEEGQPNYIEQSVN
                     AILREAELDTRVAGKSVLPKAGEYTVQALRTGIVRFLAGYDPTYRAPTDPVSAVAGAL
                     QKIRGRVPARPPGLCTGCPERPVFSSLKMLQKEMGDFHVSADIGCHLFSILPPFEIGN
                     TTMGYGLGAAGASAFNTPSDKPAIAIMGDGGFWHNGLTSGIGNAVFNKSDGLTIVVDN
                     NYAAATGGQDLLSTKSGSLKRSMRHPIEKAVRGIGVEWAKTVTTYDIASMRKALKEAV
                     TKKGTGPKVLVAQSECMLNRQRREKPQFEKSVQQGRRVVRDRFGVDADTCTGDHACVR
                     LSGCPSLTIKPNPDPLKSQPVSYVDNSCVGCGHCGEVAHEAVLCPSFYKARLVFNPGT
                     SDRLLWKLRQGVIGWLSRRNERRFAQAL"
     gene            complement(8373..9155)
                     /locus_tag="VFY27_03340"
     CDS             complement(8373..9155)
                     /locus_tag="VFY27_03340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5764573.1"
                     /translation="MTTSALHQLVAGIAAKTIRVVDLTNTLHDDTPVLQLPPQWNLHW
                     PFSLEQISQYDERGPAWYWNNFKCSEHTGTHFDAPIHWVSGKDFESNATDSIPVEKFI
                     APACVMDVTDEASTDPDFLLSIDHVRSWEAAHGTIEAGSWFLLRTGWSNRSSKDDYLN
                     IGEDGAHTPGPHEDVVKFLAGERNVIGFGTETVGTDAGQAFTFEPPFPCHNFMHGANK
                     FGLASLTNLDQLPPKGAVILAAPLKIVKGSGSPLRVLALIEE"
     gene            9497..10471
                     /locus_tag="VFY27_03345"
     CDS             9497..10471
                     /locus_tag="VFY27_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5764574.1"
                     /translation="MHAIRYHKTGPADVLRWEKVEVPRPGAGEVLLANSAIGTGLKET
                     GERQGTYPCPPLPAIPGIAAAATVLEVGAGVTDLEPGDRVAYATLPAGSYCEQRVLPA
                     ERLVPLPDDIDDRTAAAVINKGITANFLARKTYPIQRGDIVLIHAAAGGVGSLLCQWA
                     KHLGATVIGTVSTDEKAAVAAKNGCDYPIVYTKQDFVAPVREITGGLGVPVVYDSVGK
                     DTFERSLRCLQPLGMMVLYGIASGHPPPLELMKFDIWTAWYFTRPSFYVHTKSRDLLL
                     EHAATVFEMIRSGAVKVTISAEYPLKDAAEAHRALESRRSTGSIILLT"
     gene            complement(10562..11935)
                     /locus_tag="VFY27_03350"
     CDS             complement(10562..11935)
                     /locus_tag="VFY27_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX5764575.1"
                     /translation="METPRHHETWSSRTAFLLAAIGAAVGLGNIWRFPYLAGENGGSA
                     FVLVYIVCIVVVGVPVLIAELLMGRRGGMSPVGTMSVLASEANTSRHWRKAGWLAVAT
                     IFLAGTFYYVVASWVLAYVALAIEGAFSGIDASGTSALFAQLLNDPLRLSLWFGIFMA
                     MTIFVVASGIRNGLERAVRFLMPSLFALLIIMVLYAAVAGDFARGWHFLFRFDVAKIT
                     PTVVLAAIGQAFFTLGVAQAVMMTYAAYMPKHVKIPQAALVIAAADLVAAILAALMIF
                     PIVFAAGLPTDSGPGLIFETLPIAFGEMPGGRLFGSLFFVLLTIAALTSTISGVEPIV
                     SWAEEHHGWRRRPVSIFVGLAIWLVGLTSVFSFNIWNGFTPLGMFRAFHGKTLFDIFE
                     YLTVNVMMPLNGLIIAVFVGWLMSRASIADELALADGKMLSALRLLLRIVAPVAIVAI
                     FVFNIAQ"
     gene            12114..13100
                     /locus_tag="VFY27_03355"
     CDS             12114..13100
                     /locus_tag="VFY27_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5764576.1"
                     /translation="MLALEPPRLDDVRAAANRVHAHVIRAPLVRLNADLPRTEIYLKL
                     ESLQPTGAFKIRPAANAILNAPPRLVAEGVYTASSGNMAQGVAWMARTLGLAATVLLP
                     RDAAATKVAALRRLGATIRVLRDSEWWQVLADHGDTGSRGLFVHPVANQDVINGNATI
                     GLEIHEELPDADTVLVPFGGGGLSSGIASAMRAAGSRARILGAESDHCTPLAVAQEAG
                     KPLSVPIRPCFISGIGVGRILDEMWPLIKELVDGAVVASTEEVAAAIRLLFERHRVIA
                     EGAGAAPVAAALAGRAGTGKIVCVVSGGNLDERYLTDILQGRPARMINRRTQ"
     gene            complement(13094..14155)
                     /locus_tag="VFY27_03360"
     CDS             complement(13094..14155)
                     /locus_tag="VFY27_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5764577.1"
                     /translation="MTTPNIARLKPYLIAVEKDKAYLWCACGRSKAQPFCDGSHKGTG
                     FLPLKWIADSTGEKLFCACKHTLSAPFCDGTHNRLSDTYAEADLSDRAGAALVEYAQL
                     GSGALKASLDNGCYVIRVPGESMVDVRGMLVYPVIGTADGAEHISQFFASVRTGASPV
                     VRYPGSDVVLFVHTGTGIVKIADRSFAVAPETGVCIKPGEGFQIVNDDEHPMSLSISV
                     CPPCAAPEYLDRMPEIFDDAVPNRVQGVDESKQEAMADRFFQVLIGDKSHGTPVTQFI
                     GVIPLSRAAHHRHLYEETITILSGEGFMWTDETKTAVRAGDTIFLPLKQAHSLECTSP
                     DGMRLLGLFYPSMSPAVNY"
     gene            complement(14185..15360)
                     /locus_tag="VFY27_03365"
     CDS             complement(14185..15360)
                     /locus_tag="VFY27_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5764578.1"
                     /translation="MNIKPDLTTRAAEPDWTAIRARFPIFERKTYLNSCAYGALATEV
                     MAALQKYIADRLEKGTDWNYWVARSDAARVAVADFLGAETDEVAVTTSASAGINSIAS
                     ALSFDRPRNKVVISDFEFPTNAQIWYAQEQRGAKVVRIPEENGYIPVEKFEAAIDEST
                     LIVAVTQVCFGNGARLDIPAIVEIARNKGALVLVDGYQGLGTFRFDVRRVRPDFVVGG
                     MVKYLLGTAGIGFLYVRRDLIEHLVPTVTGWFAQSDIFAMDVTRYDPAPNARRFEMGT
                     PPVINCYAAEAGIKLLQEVGMSLIETRLAELTTAIVAEARSAGYKLAVPDDPGRRGAL
                     ITLRTHDEDALVGWLEEHDIVTSSRRGNLRISPHFYNEHEDIDKLFRALRKQRHLLI"
     gene            15465..>15856
                     /locus_tag="VFY27_03370"
     CDS             15465..>15856
                     /locus_tag="VFY27_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5764579.1"
                     /translation="MPIAGTGDKWLQSFVPYLLYRITNQLTRRIRSRLRREGINISRW
                     RVLAVLRAYGDLNLGAIVELTVMEQPSVSRIVAQLEQEGLVRRKVSRKDSRFVNVSLT
                     SAGAKAFQTIYPAAKKHQERALRGFSRQ"
BASE COUNT         3139 a         4765 c         4764 g         3188 t
ORIGIN      
        1 gccgaatgtg tcgtcggggc gattgcgctc gtagacggta atgtcccacg caggccatgc
       61 cttcttcgcg agaattgcga agtagaggcc gccaggacct ccgccaacga tggtcgtttt
      121 catgagagat tgttttatct atagatagtt gaagtataaa gtatcaggcc gacgctgtct
      181 cggcaaggtg gcggacgagc tgcgagcgca atgccggagg cagctgcgcg ctgcggaatc
      241 catcttgctc caaccttaca aaaacaatcg cttgtgaggt tctggccgcg catccccccg
      301 gaccgaccag ccacgattcg aaatgcaccg aactgcgccc gatcctggtg gggcggatcc
      361 atacgctgat cgtctccccg ctgcgcggaa gttctgcaca gactgtgcgg agcgagaccg
      421 tcggaatgcc ggctcgatcg cggtgcaggc ggctgaaagg cagcatgagc tcgttctcga
      481 accactgttc gacggccgcg ttgacgatct cgtagtagcg ggaggtctgc agcctggcgt
      541 ccggaccggc agcccaatct tctatcaaca tcgcttccga gcggaatgcc ggcctggcag
      601 ggtcgtgatc ctgaaggccc gacagcaggt catcagcggg ttgccacaac atcgtgaact
      661 gttcctcacc gccggccacg cctgagacac gccagccttc tcgcagtgcc gaggcggtga
      721 gagcgagctt caagcgtgta ccgagcggcg tcggtttcct gaacccgata tccagagtga
      781 acggctcttt cgcgagcgcc agttccggga acgcctgtcc gagcatctcc aggtagcgcg
      841 ggtaaaacac gatgcctgca gcatcgatgt gcgcaaagcg tacgaccact tcacgccagt
      901 gctccgcacg tgcggcccgg aggatcggtg cacctggatc ggtcatattt tgagtgcaaa
      961 ataaagcagc ataaacgtta taatcagcgt ccttttaaat tgaacaagtt tcacggcgga
     1021 tcgttgccgg cggaactggg gtgccggcga cgggttgcct atgtccagga agatcgcaga
     1081 gtctcaacat tcgcacagca aagagggtat ccgtacctgg ctaagattgc tgtcttgcga
     1141 gaaaagtatc gaacaggaat tgcgcagccg ctttcgcggc cattttacgg tcacactgcc
     1201 gcagttcgat gtgctggccg agctcaagcg ggctggaagg ccactgacga tgtcgcagct
     1261 ttccagcgaa ctcatggtgt cgaatggcaa catcaccggc gtggtagatc ggctggagaa
     1321 gggcgggctt gtcagccggg accgctgcaa caaggatcgc cgtgtccagt tcatcgcgct
     1381 gacgccgctg ggcgcaaagc agttccagca aatggcccgg cgacacgaga agtggctggc
     1441 cgagctgttc tccgaattga cggtgaacga catggatgac ttgcagaagc tgttgttgaa
     1501 ggcacggcgg tcggcaacga caggcacggc ggcctgaccc tgttgctacc ccgcttttcg
     1561 ctcacttcct tgaagccggg ccgacgactg cagtcgtctc gatttcgatc aacgcttccg
     1621 gctcgaccag gtcgctgact ccgaccacag ccatcgtcgg gaaattcttg ccgatgatgt
     1681 ccaggtagac gcgcccgacg tcgctcatgt tgttgcgata caggtccagc cccttgacat
     1741 accatgtcat gcgcacgatg ttgccgggcc cagtaccgcc ggcttccagt acggcgaccg
     1801 tattcttcag cacctgcgca agctggctga cgaaatcgac aggaaaccgg ccgtgttcgt
     1861 cccagccgat catgcctgcg acaaataaga actggccttc ggcaaggatg ccgttcgagt
     1921 aacccttcgg ccgcggccag ccctccggta acaggtgctt cattgcgtga cttctgcgtt
     1981 tgcataggtc tgcctggcga tgatgacctt ctgtatttcg ctcgcgcctt cgtagatccg
     2041 cagcgcgcga atttcccggt acaggctctc cgcaatattg cctttcgtaa caccccggcc
     2101 gccgaacagt tgcacggcgc ggtcgatgat ccgttgcgcc gcctcggtgg cataaagctt
     2161 cgccattgcg gcttcccgtg taacccgctc ctggtagcaa tccttggccc aggcggatct
     2221 atatatgagg agggcgctcg cgtcgacctc gagcgccatc tcggcgatgc tcgcctgggt
     2281 catctgcagt gcgctaaggg gggcgccgaa aagtgtacgg tggctggtat ggctgacaga
     2341 ttcatccaac gcacgtcgag caaaaccgag tgcggcacct gcgaccgagg tccgaaatac
     2401 gtccagggtc gccattgcga tacggaagcc cttcccgggc tcgcctatcc gcgccgaggc
     2461 cggtatgcgg cagtcctcga agcgcagtgt ggcgagcggg tgtggcgcaa ttgtctcgat
     2521 gcggtccgct atgcggaggc cgggcgcttt cgcgtcgacg atgaacgcgc tcagtcccct
     2581 tgcgcctggc tcggtcccgg agcgcgcgaa aacacagtaa aaatccgcga taccgccatt
     2641 ggaaatccac gtcttttcgc cattcaatac gtactcgtca cccgacagct ccgcactcgt
     2701 ttgcaatgcc gcaacatccg atccggcgtt ggcttcggac agcgcgaacg cggctatcga
     2761 atggccagat gcaacggacg gcaggtattg ctcgcgctgc gcatcattac cggcaagagc
     2821 aatcgcgcca cttcccaggc cctgcatggc gaacgagaag tcggcgaggc cggagtggta
     2881 ggccaggatt tcgcggctga gcgcgatgct acgcacatcc agtgcatccc ttgcgccgcc
     2941 ccatgctgcc ggtacagagt ggcgcagcca gccggcgtgg ccgaggtcgt taacgagctt
     3001 gcggcacgcg gtgtccgtgt cgtcgtcgtg ttcgaaatcg tacgaatcgc accattccga
     3061 cagccgggca tgcaggatac gatgctcgtc atccaggaac ggccagtcaa ggtaagtccg
     3121 gtccgccatc agttaccctc gaattccggt ttctgccgcg ccacgaaggc gcgataggcg
     3181 cgctcgaagt cggcagtctg catgcaaatg gcctgggctt gagcttcagc ttcgatcgcc
     3241 gtgtcgagcg acatgtccca ctcgatgttg agctggtttt tcgtgatgcc gtgcgcgaag
     3301 ttcggtcccg cggccagcct ggccgcctgt gagcgcgact cctcgagcag ggaagcgggt
     3361 tcatagagcg cattgaagaa tccccagcgg caagcctctt ctccactcat cgatcgtccg
     3421 gtgaaaagca gttccgatgc gcgtccctgg ccgatgattc gcggcagcat tgcacaggca
     3481 cccatgtccg ctcccgccag gcctactcgc gcgaaaagaa aagccgtctt cgcgcgtgcc
     3541 gtgccgtagc gcaaatcgct tgccatggca attgcggcgc ccgcgccggc gcaaacacca
     3601 tcgatggctg caatgaccgg ctgcggacat tgccgcagcg ccttgacaag atcgccggtc
     3661 atgcgcgtga agcgcaacat gcctttcatg tccatcttcg tcaggggtcc gatgatttcg
     3721 tgcacgtcgc cgccggaaca gaagttttcg ccagccccgg caatcacgac ggctcgtacc
     3781 gaatcttcga agacgagcgc acgaaaagta tctctcaact cggcatacga ctcgaacgtc
     3841 agaggattct tacgctccgg acggttcagc gtgactgtcg cgacgccgtt ggactccacc
     3901 tcccaaagaa agtgttccgg tttgattgtg ctttcagtca ttacatgacc tctccgccgg
     3961 caatggcgat tgcctggcca gtgaccgagg cggcgttcgg gccgcatagc cacagcacgg
     4021 catcggccac ctcgtccggt tttaccaggc gtcgttgtgg attgactttc agtaattcgg
     4081 ccattgcgtc atcgcgattg cggccggtgc tcgccacgat gttgtcgata gatcgctcga
     4141 ccagttcggt atccgtatag ccggggcaga ccgcgttcac cgtgacgccc ttccgagcga
     4201 attcgagtgc cagtgaccgg gtcagtccca gtacgccatg cttcgctgcg cagtaggcgg
     4261 agacgtaggg atagccggtc agggaagcgg tactggcgat gttgacgatg cgcccgtcgc
     4321 cggcggccac catgtacccg ataaccgcgt gcgtgcagag ataggttccg gtcaggttga
     4381 cggcgagcat ccggtcccaa agcgcttcgt ctgttttgga aaatggcgca gcttccgccg
     4441 agcccgcatt gttgacgaga atacgtatcg gacctcgagc ggcgacggcg tccgagattg
     4501 ccgcttcgac ctcgccacga actgtgacat cggccatggc ggcgtgtatg cccagtcgct
     4561 cagccgttgc acgaagcttg tccggattgc ggccaacgat cgtcaccggc gcacccgcgt
     4621 ccgcgaaccg ttcagcaatt gcggtgccga tgccgccggc gccgcccgtc accacgacat
     4681 gccggttctc gaaggttgat ggaaaggcgt aagtcaacca ttaatctccg agagtgtttt
     4741 ctcgaaagca gatccctgtt cgtcggccaa tgccgcgccg tgcaggcgcc gcagcaggtc
     4801 tgccttggtt ccgggagcgg cacggcgtga tgcctctaac atcgtgcggt atcgcttcaa
     4861 gccgcgctca tacgtctcgc cgtacccgcg gacgatctcg atactcccgg caagcgcgac
     4921 ggcgccttcg tagtccaccg tcgcggtttc tgcgacggca gccagccacc cttctatatg
     4981 ctgctgctgc acggtgtaac gcaaggttcc ccggcgccac cgccggagtc gcgccagggt
     5041 gctcagcaag acatagcccc agatcttgtt ggtgttcagg ttacgcccgg tccggaacag
     5101 cggcgctgtc cagcgcctgg ctgccggcga ggcgaccgcc cgcgcaccca gtcccgctgg
     5161 aagcgtgtcg cacatctcct cgaagcgcgg gtgaaaatac tcggtgacgt accagggttg
     5221 tccggaagcc gcgccgatat ccctgccgat gccggcgagc ctgtcggtcc gggtttttat
     5281 ctctgccacg cgaatcgtat cttcgtagca catctgcaag gcaagttccc gcgcaaccgt
     5341 ttcggtcagg tctggattgg cactggcatg cggctgactg cgatccagcg actctatccg
     5401 ggcgaggcgc tccaggtaca ggtccgcata ggcctggtcc tggtatgaca gcgttcgcaa
     5461 ggctccatgc agcgccatgt tacggcaacc tgccggcaac atgccgtcga tgcgaaccgc
     5521 gagggccttc cctgcgggac ccttcggcat tgcaagcggc atctgctcaa cgactgccgt
     5581 tgtacttgta tggttgtgcg ctcctgcaaa gcctgccgcg aagccggcga ggttcgtacc
     5641 gaccgcgcgc ccggatgcgc ggattacctc ctcatactgt tcgcgttgaa acggcagtgc
     5701 gccgctcccg gctactgcgc cgaaaatcac ggcactgata acgctggacg tttcccgcgc
     5761 ccgggcggcc atatcgaaac agatgaatct ctgtgccgct tcccgcgcgg cctccatcac
     5821 gggttgcagg tccatgatgc cgtcgcccag tgcagtcttc tcactgatgg catagtcacg
     5881 gtgggagctc gcgatcagcg tcgtgatgtt cggcgtaacg aagcctttct ccagcgcgcg
     5941 gccggtctcg gcgagttcgg aggcgatgac gatgtcgaca tcgcccggca cgggatactg
     6001 ggcgaaaacg ggcgggcgtc cttcagcctc ggcggctgca cgccgcagca tctccacgca
     6061 atagactgtt gccccggtcc gttgcgcgac cccggcaacg tacgtcgact gggccaggaa
     6121 gccgtttgct tcggcaagat ccaccagcca gttcgacagc acgccgccgc cctgtccgcc
     6181 gagcgcgagc attgcgatcg tgatcggccg ctcgcctgtt gcggccacgt cagagtgcct
     6241 gcgcgaagcg tcgctcgttt cgccggctca gccagccgat cacgccttgc cggagcttcc
     6301 agagtaaccg gtctgatgtg ccggggttga acacgagccg cgccttgtag aacgatgggc
     6361 aaaggaccgc ctcgtgcgcg acttcgccgc aatgtccgca gccgacgcaa ctgttgtcga
     6421 cataggaaac gggctgcgat ttcaacggat ccggatttgg cttgatcgtc agggaggggc
     6481 agccgctcaa ccgtacgcag gcatggtctc cggtgcaggt atccgcgtcg acgccgaaac
     6541 gatcgcgaac gacccttcgc ccttgctgta ccgacttctc aaactgaggt ttctcccggc
     6601 gctgccggtt cagcatgcat tcggactggg cgaccagcac tttcggtccg gtgccctttt
     6661 tagtcaccgc ttctttcaga gctttgcgca tcgacgcgat gtcgtaggtc gtgacggttt
     6721 tggcccactc gacgccaatg ccgcggacgg ccttttcgat gggatggcgc atgctgcgct
     6781 tcagggaacc ggatttcgta gataagagat cctgtccgcc ggttgccgcc gcgtaattat
     6841 tgtcgacgac aatcgtcagc ccgtcactct tgttgaacac ggcgttgccg atgccgctgg
     6901 tcaggccgtt gtgccagaag ccaccgtcac ccatgatcgc gatggccggc ttgtcggacg
     6961 gcgtgttgaa ggcagaggcc cccgctgcgc cgagaccgta tcccatggtg gtattgccga
     7021 tctcgaacgg cggcaggatg gaaaaaagat ggcagccgat atcggcgctg acgtgaaagt
     7081 cgcccatctc cttctgcagc atcttgaggc tgctgaatac cggacgctcg ggacagccgg
     7141 tacaaagccc gggcgggcgg gcagggaccc tgccacgaat cttctgcagc gctcccgcga
     7201 cagccgatac aggatcggta ggtgcacgat atgtcggatc gtaaccggca aggaaccgga
     7261 cgattccggt gcgcagcgcc tgaaccgtgt attcacctgc ctttggcaac accgacttgc
     7321 ctgcaacccg cgtgtccagt tcggcttccc gcaaaatggc attgaccgac tgctcgatgt
     7381 aattcggctg gccctcttcg acgagcagca ccgcgcgttt gccgcgacag aaatccacga
     7441 gctcgtcgtc aaccagcggg tacgcaacgt tcatgacgta gagcgggatc tgcgtcctgc
     7501 cgtaaatatc ggccaggttc agccgctcca gtgtcctgat cagggagttg tagaggccgc
     7561 cctggacaat gatgccgatg tcaccgcgtt caccgtcgaa aacctcgttg agcccatgct
     7621 ccttgatgta gcggatcgcg gcaggccagc gttcgttgac tttctcctgc tcgtgcagga
     7681 aagaggcagg cggcaagacg atccggtcaa cgtcgcgtct tggattctcg accgcctcgc
     7741 ggagcggcat gcgcggcggc tggttgtctt tcgcgacaaa gcgtccgtgc acatgacagg
     7801 agcggatccg catctcgaga aacaccggcg tatggctcgc ctcggagagt tcgaacgcgt
     7861 gttccacggc atcgacgatg ctcggcaggt tcggtcgggg atcgatcagc cacatctggc
     7921 tcttcatggc aaacgcgtgc gtccgctcct gcatgatgct ggagccttcg ccgtagtctt
     7981 cgccaacgat gacgaccacg ccgcccgtca ccccgccgga ggcgagattg gcgagggcgt
     8041 cgctggcaac gttcgttccg acagtggatt tccacgtcac ggcgccccgt agcggatagt
     8101 tgacggatgc ggccgccatc gctgccgccg ccgcctcgct cgcgctcgac tcgaaatgca
     8161 cgccgagttc gccgaggatt tcctgggcat cggagagcac gtccatcagg tgggagatgg
     8221 gcgatccctg gtagccgccg acgtatgcca cgccggactg cagcaacgct ttggtaacgg
     8281 cgagaatgcc ctcgccggcg aattcctcgc cggcgccgat cttcagtttc tcgacctcct
     8341 tcttgaatga tcgctctgcc atagcgacta atttactctt cgataagggc gagtacgcgg
     8401 agcgggctgc ccgatccttt cacgatcttc agcggggccg ccagtatgac ggcgcctttc
     8461 ggcggcaact ggtcgaggtt cgtcaggctt gccaggccga acttgttcgc cccgtgcatg
     8521 aaattgtgac acggaaacgg tggctcgaac gtgaatgcct gcccggcatc ggtgccaacg
     8581 gtttccgtgc caaagccgat tacgttgcgt tccccggcaa ggaacttgac cacgtcctcg
     8641 tgaggacctg gcgtgtgggc gccgtcctcg ccgatgttga ggtagtcatc ctttgacgaa
     8701 cgattcgacc accccgtgcg aagaaggaac cagctcccgg cttcgattgt gccgtgtgcg
     8761 gcttcccagg accgcacatg gtcgatgctc aggagaaaat ccggatctgt cgaggcttcg
     8821 tcggtcacgt ccattacgca tgccggggcg atgaacttct ccacagggat agagtccgtc
     8881 gcattgcttt caaagtcctt gccacttacc caatgaatcg gcgcatcgaa atgcgtgccg
     8941 gtgtgctcgc tgcacttgaa attgttccag taccacgccg ggccgcgctc gtcgtattgc
     9001 gatatctgtt cgaggctaaa cggccagtga aggttccatt gtggcggaag ctggagtacc
     9061 ggggtgtcgt cgtgcaaggt gttcgtcagg tcgacgacgc gaatagtttt cgcggcgatg
     9121 ccggcgacga gttggtgcaa cgcgctcgtt gtcatttgat gcccctttgt tcggcaagac
     9181 ggcggctccg accttaactc agtggactgt tccaggtttc gcaggccggg gcaaggccgc
     9241 aaataattat tttatagtta aactttccaa ctgtaaatga cgtcgccgcg gccttaccgt
     9301 caggcattcg agcgagtgtt catcggcgtt acgagttggg caaggcgatc gacgttgttt
     9361 ttctgttgag ttgacgagag ggtacgggta ctctccaagc cccccgagtg tgacgcatag
     9421 cgtcggggta gtggtgcgca gcaagttgct gctgaggtct ggactcaagc gttaacagtc
     9481 actctcggtt gttccaatgc atgctatcag gtatcacaag accgggccgg ccgatgtgct
     9541 tcgttgggaa aaggtcgaag tgccgcgtcc tggcgccggc gaagtcctgc tagcaaattc
     9601 cgcaatcgga accgggctga aggaaacggg cgagcgacaa ggcacgtatc cgtgcccccc
     9661 actgcccgcg attcccggaa ttgcggcggc ggcgacggtt cttgaagtcg gggccggcgt
     9721 tacagatctc gagcccggtg acagggtagc ctacgctaca ttgccggccg gcagttactg
     9781 cgagcagagg gtgttgccgg ccgagcggct ggtcccgctg cccgacgaca tcgatgacag
     9841 aactgcagca gcagtgatca acaaagggat tacggcgaat tttctcgccc ggaagaccta
     9901 tccgatccag cgcggggata tcgtcctcat ccacgccgcc gccggcgggg tggggtcgct
     9961 cttgtgccaa tgggcgaaac atctcggcgc gaccgtcatc gggacagtca gcaccgacga
    10021 aaaagctgcc gtcgcggcaa agaacggctg cgactatccc atcgtttaca caaagcagga
    10081 tttcgtcgcg ccggtccggg aaatcactgg cggtctcggc gtcccggttg tttacgactc
    10141 cgttggcaag gacacatttg agagatctct gcggtgcttg caacccttgg gcatgatggt
    10201 gctctatgga atagcctctg gccatccgcc gccccttgaa ttgatgaaat tcgatatctg
    10261 gacagcctgg tatttcaccc gcccgtcgtt ttacgtgcac acgaaatcgc gtgacctgct
    10321 cctcgagcat gccgcaacgg tcttcgaaat gataagaagc ggcgccgtga aggtcactat
    10381 ctcagcggaa tatcctctca aagatgccgc agaggcgcac cgcgctctcg agtcacgcag
    10441 atccacaggc tcgatcattc tgctgacatg attcacgagt gcgaactgca tcgaacgttc
    10501 cagttcgggt ctcagcggga acaacctgcg tgcacgattc atcgcgccgc tcgacatgtg
    10561 attactgggc aatattaaac acgaagatcg ccacgatcgc gacgggcgca acgatccgca
    10621 gcaggagccg cagggccgaa agcatcttcc catccgctaa cgccagttca tcggcgatgg
    10681 aagctctgga catcagccag ccgacaaata cggcgatgat caagccattg agcggcatca
    10741 tcacgttcac cgtcaggtat tcgaagatat cgaacagggt tttcccgtga aaggcccgga
    10801 acatgccgag aggagtgaat ccattccaga tgttaaagga gaagaccgag gtgaggccta
    10861 ccagccagat cgccagtcca acaaagatgg agactggcct ccgtctccag ccgtgatgtt
    10921 cttccgccca ggagacgatg ggttcaactc cggaaatagt ggatgtaaga gccgcaatag
    10981 tgagcagtac gaagaacagg gatccgaaaa gtcgcccgcc cggcatttca ccgaaagcaa
    11041 tcggcaatgt ctcgaaaatc agcccgggac cggagtcggt cggaagcccg gctgcaaaga
    11101 cgattggaaa tatcattaac gcagcgagga ttgcagcgac gagatccgcc gcggcgatca
    11161 ccaaggctgc ttgcggaatc tttacgtgct ttggcatgta tgccgcatac gtcatcatca
    11221 cagcctgggc cacacccagc gtgaagaatg cctgacctat ggctgcgagc acgacagttg
    11281 gtgtgatttt agccacgtcg aaccgaaaca ggaaatgcca gccgcgcgcg aaatcccctg
    11341 cgactgcagc atacagaacc atgatgatca gcagtgcaaa cagggatggc atcagaaagc
    11401 ggacggcccg ttcaaggccg ttgcgaatgc cgctggccac gacaaaaatc gtcattgcca
    11461 tgaatattcc gaaccagagc gacaaccgga ggggatcgtt aagcagttgc gcgaataacg
    11521 cgctggtgcc cgaggcgtcg atgccggaga aagcgccctc gatcgccagc gcaacgtacg
    11581 ccagcaccca acttgccacc acatagtaga aggtgccggc caggaaaata gttgccaccg
    11641 cgagccagcc ggccttgcgc cagtggcgac tcgtgttggc ctcgcttgcg agtaccgaca
    11701 tggtaccgac ggggctcatc ccgccccggc gtcccatcag cagttccgcg atcaggacag
    11761 gaacccccac aaccacgata caaacgatat aaacgagaac aaacgcagag ccgccgttct
    11821 cacctgccag atagggaaac cgccaaatgt ttccgaggcc gacagcggcg ccgattgcgg
    11881 ccagcagaaa cgccgtgcgt gacgaccagg tttcgtggtg ccggggagtt tccatcggat
    11941 gaattttctc gcctgaacaa gatgctatta ttcattcttt gattcgaagc taaaccggca
    12001 cttgtcgagc tgtcaagaaa ccttcaagga ataagggccg ccgacgccga tgtggaaagt
    12061 gcacgcttcg ttattcacac gagcgccctc gtttgtattc ctgcaagtgc caaatgctgg
    12121 ctcttgagcc accgcgcctt gacgacgttc gcgctgctgc aaacagggtg cacgcgcatg
    12181 tgatacgtgc gccgctcgta cggctgaacg ccgacctgcc gcgcaccgaa atctacctga
    12241 agctggaaag cttgcagccg acaggcgcgt tcaaaattcg tcctgccgct aatgccatat
    12301 tgaatgcgcc tccgcgattg gtcgcggagg gtgtctacac agcgagttcc ggtaacatgg
    12361 cgcaaggcgt tgcctggatg gccaggactc ttgggctcgc tgcgacagta ttgcttccgc
    12421 gcgacgccgc cgcgacaaag gtcgcggccc tgcgacgcct gggagcgact atccgcgtgc
    12481 tgcgggattc ggagtggtgg caggttctgg cggatcatgg ggatacgggc agccggggct
    12541 tgttcgtaca tcccgttgcc aatcaggatg tcatcaacgg gaacgcgact ataggactcg
    12601 aaatccacga agaacttccc gatgccgaca ctgtgctggt ccctttcgga ggcggtggac
    12661 tctcgagcgg tattgcctca gccatgaggg ccgcaggctc gagggcgcgg atattaggcg
    12721 ccgaatccga tcattgcaca ccgctcgcag ttgcacagga agcgggcaag ccgctgtccg
    12781 tgccgatcag gccctgcttc atttcaggaa taggggtcgg ccgcatcctc gatgaaatgt
    12841 ggccgctgat aaaagaactg gttgatggtg ccgtagtcgc tagcaccgag gaagttgctg
    12901 cggcgattcg cttgttattc gagcggcatc gcgtgatcgc agagggcgcc ggtgctgctc
    12961 cggtcgcggc agcactggcc ggacgagccg gtaccgggaa aatcgtatgc gtcgtctcag
    13021 gcggcaatct tgatgaacgc tacctgaccg acattctgca gggtcgcccg gcacgtatga
    13081 tcaaccgccg gactcagtag tttactgccg gcgacatcga tgggtagaac aggccgagca
    13141 ggcgcatacc gtccggcgac gtgcattcga gggagtgtgc ctgcttcagg ggcaggaata
    13201 tcgtgtcacc tgcccgcacg gcggtcttcg tctcgtccgt ccacatgaat ccttcgccgg
    13261 acaggatggt aatcgtttcc tcatagaggt ggcggtgatg ggcggccctg gaaagcggta
    13321 tcaccccgat aaactgcgtg acgggtgttc cgtggctctt gtcgccgatc agtacctgga
    13381 agaagcggtc cgccatcgct tcctgcttgc tttcatcgac gccctgtacg cgattcggaa
    13441 cggcgtcatc gaagatctcc ggcattcgat ccaggtactc gggcgcggcg cacggcgggc
    13501 agacggagat gctcaaggac atgggatgct catcgtcgtt gacgatctga aagccctcgc
    13561 ccggcttgat gcagactccg gtttccggtg caacggcaaa gcttctgtct gctattttga
    13621 cgattcccgt gcccgtgtgg acgaaaagta caacatcgct gccggggtat ctgacaaccg
    13681 gggatgcacc ggtccggacc gacgcgaaaa actgggatat gtgctcggcg ccgtccgccg
    13741 taccgatgac cgggtaaacc agcattccac gcacgtcgac catcgattcc ccgggaaccc
    13801 ggatgacata gcagccattg tcgaggctcg ccttcaatgc accgctccca agctgtgcgt
    13861 attccacaag cgccgcacct gccctgtctg aaaggtcggc ttcggcataa gtgtccgaaa
    13921 ggcggttgtg ggtaccgtcg cagaatgggg cagacagcgt atgcttgcag gcgcaaaaga
    13981 gtttttcgcc cgtactgtcc gcaatccatt tcagcgggag gaaccccgtc cccttgtgcg
    14041 acccatcgca aaacggctgg gccttgctgc gcccgcatgc gcaccacagg tacgccttgt
    14101 ccttttcaac tgcgatgaga tatggcttca gtcttgcgat gttcggggtc gtcatctgga
    14161 cgcgtgctca gggtgatgat actttcagat aaggagatga cgctgcttcc gcagtgcacg
    14221 gaagagctta tcaatatctt catgttcatt gtaaaagtgc ggtgatattc tgagattgcc
    14281 ccggcgggac gacgtaacga tgtcgtgctc ctccagccaa cccacaagcg cgtcttcatc
    14341 gtgcgtgcgc aaggttataa gggcgccgcg tcgtccagga tcatcgggca ctgccagctt
    14401 gtagccggcc gacctggctt cggcgacgat ggcagtcgtc agttcggcta accgggtctc
    14461 gatcagggac atgccgactt cctgcagcaa cttgatcccg gcctcggcgg cgtaacaatt
    14521 aatgacggga ggagttccca tttcgaatcg gcgcgcattg ggcgccgggt cgtaacgcgt
    14581 cacgtccatt gcaaagatgt cggactgggc aaaccatcct gtgacggtag ggacgaggtg
    14641 ctcgatgagg tcgcggcgta cataaaggaa tccgatgccc gccgttccga ggaggtactt
    14701 gaccataccc ccaacgacga aatctggacg gaccctgcgt acgtcaaatc tgaaggtgcc
    14761 gagcccctgg tagccgtcaa ccagtaccag cgcgcctttg ttccgggcga tttccacgat
    14821 ggccgggatg tcgagtcgcg ctccattgcc aaagcacacc tgagtaacgg caacaatgag
    14881 agtgctttca tcgatcgctg cctcgaactt ttcaaccggg atgtagccgt tctcttccgg
    14941 aatccggacg accttcgcgc cgcgctgttc ctgcgcgtac cagatctggg cattggtcgg
    15001 gaactcgaaa tcgctgatca cgaccttgtt ccgcggccga tcgaacgata atgcgctggc
    15061 aatggagttg attccggccg aggcagaagt ggtgacagcc acttcgtctg tttccgcgcc
    15121 gagaaaatct gcaactgcta cgcgtgcggc gtcgctgcgt gcgacccagt agttccagtc
    15181 cgtccctttt tccaggcggt cggcaatgta tttttgcagt gccgccatta cctccgttgc
    15241 aagggcgccg taagcacagc tgttgagata ggtttttcgc tcgaatatcg ggaaacgcgc
    15301 cctgatcgcc gtccagtctg gctccgctgc acgtgttgtg agatccggct tgatgttcat
    15361 cgtgtccaga aaatattaga gggaaggcaa caggctgctc gatggagaaa gtacatgaga
    15421 tcatatatat tcacaaagtc tactaatttg ccagggaccg cgagatgccg atagcaggta
    15481 ccggtgacaa atggctccag agctttgtgc cgtacctgtt gtatcggatc accaatcaac
    15541 tcacgcgcag aattcgcagc cggctgcgcc gggaagggat caacatttca cgctggcgcg
    15601 tgctggcggt attgcgcgca tacggggatt tgaacctggg cgcaattgtc gagttgacag
    15661 tcatggagca gccgtcggtc agcaggattg tcgcgcagtt ggagcaggaa gggctcgttc
    15721 ggcgcaaggt gtcacgcaag gattcgcgct ttgtgaatgt cagcttgacg agcgccggag
    15781 cgaaggcctt tcaaaccatc tatcctgctg ccaaaaagca ccaggaacga gccttgcgcg
    15841 gcttctcccg ccagga
//
LOCUS       DASUOM010000166         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_506695,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000166 DASUOM010000000
VERSION     DASUOM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506695"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..929
                     /gene="rdgC"
                     /locus_tag="VFY27_03375"
     CDS             108..929
                     /gene="rdgC"
                     /locus_tag="VFY27_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX5764580.1"
                     /translation="MFRSVRFFRLSSAWPESDQALSQLLASAEFQPCGAWSETSSGWE
                     SPAGDADAALSRRVDGADLLRLRTQARLLPRAAIEDALEARIGEYRERAGEEPTRTAR
                     RKLREQTRDELLPKAFVRSQRTSGFVIPSEGIVAVDTLSDSRAEHFLEHLRAPLGELD
                     VAPLTFGRPVGELLMRIFLGDTAGEFAHGGECRMCDPADPRATIRCVDIDLADAKVRN
                     HVREGMRLTHLGIEINSILNCTLDENGGIGKLRLVGMDGGKIFPMRIHSRGSTPS"
     gene            1155..1556
                     /locus_tag="VFY27_03380"
     CDS             1155..1556
                     /locus_tag="VFY27_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764581.1"
                     /translation="MSGNLDWKWIGFGVLIMLGVSIVAGIVMGLFLMPDIQDATQVEE
                     IELSGGKMAALALVNFLVFVIGGFIVGLKSTGRTILEPGISAAIAVIVALVLAGSFTF
                     GSLIAGGLVPFLAGVLGGWMGERRQESSARP"
     gene            complement(1757..>2168)
                     /locus_tag="VFY27_03385"
     CDS             complement(1757..>2168)
                     /locus_tag="VFY27_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764582.1"
                     /translation="PPRDWFLRIVPLPRQSSAYGYAQAQDPAWKDYMKETGATWFSDR
                     DNMKDALDFIGWYNHKSHKELGISIRDPRNLYLAYHEGRSGYRSGEWRRKPDLINLAE
                     RVDRRAREYDEQLRQCEDRFKCDSWYQFWPFCRK"
BASE COUNT          415 a          644 c          674 g          435 t
ORIGIN      
        1 aaccttgcca aggcacggat accacttccg cttacttttc aatggtgaca tgacgctgcc
       61 gagaaccgat actccccgcc ctttcgcatc ctcgaccggg agtttcaatg tttcgcagtg
      121 tccgcttctt tcgtttgtcc agcgcatggc ctgaatccga ccaggcactt tcgcaactgc
      181 ttgccagcgc ggaattccaa ccctgcggcg cctggtcgga aacgagttcc ggttgggaat
      241 cgcctgcagg cgatgccgac gctgcgctaa gccgccgtgt cgacggtgca gatctcctgc
      301 gactgcgcac gcaggcgcgc ttgttgccaa gggctgcgat cgaggacgca ctggaagcaa
      361 ggatcggcga gtatcgggaa cgcgcgggcg aggaaccgac ccgtaccgcg agaagaaagc
      421 tccgggaaca gacgcgcgac gaactgctgc cgaaggcgtt cgtgcggtcg cagaggacca
      481 gcgggttcgt cattccctcc gagggaatcg ttgccgtcga cacgctctcg gattcccggg
      541 ccgagcactt cctggaacac cttcgtgcac cactgggcga gctggacgtg gcaccactga
      601 cgttcgggcg cccggtcggc gagttgctga tgcgaatttt cctcggagat acggcaggcg
      661 aatttgctca cgggggagaa tgccgcatgt gcgatcctgc agatccgcgc gcgacgatac
      721 gctgcgtcga tatcgatctc gcggatgcga aagttcgtaa tcacgtcagg gaaggtatgc
      781 gcctgactca cctcggtatc gagatcaaca gcatattgaa ctgcaccctc gacgagaacg
      841 gcggtatcgg caaattgaga ctcgtcggaa tggacggagg gaagattttc ccgatgagga
      901 tccactcgcg cggctcgacg ccgagttagc gctgctggcc ggcacaatgc ggcagttgct
      961 tgcggcactg aagaaggcac ttggcgggtt cgccggctcg gtcctcccgg caccactggc
     1021 tgccacggct gagttctatg ggcaacaccg gaccgatcgc atttcggaga tcaacccatc
     1081 tgtcagcgcg agtactatcg agcgcggttt aagatggtag gctaagcctt actcggacag
     1141 gtcaggagcc gatcatgtca ggcaatctcg actggaagtg gatcgggttc ggcgtgttga
     1201 tcatgctcgg agtgagcatc gttgccggca tcgtcatggg tctcttcctg atgcccgaca
     1261 ttcaggacgc gacgcaggtg gaagagatcg aactgagtgg cggcaagatg gcggcgctgg
     1321 cactggtcaa ctttctcgtt ttcgtgatcg gcggtttcat cgtggggctg aaatccaccg
     1381 ggcggaccat tctggagccg ggcatctccg ccgcgatagc ggtaattgtc gcactcgttc
     1441 tcgcaggaag tttcaccttc ggcagcctca ttgcgggcgg cctggtgccg tttcttgcag
     1501 gcgtactcgg aggatggatg ggcgagcggc gccaggaaag ttcggcgcga ccttaatccc
     1561 agtcagcgct gaagaaaagc cgagacctcc gcttgatcgc gcggttttat gaagccccgg
     1621 attgccagca tcaacgatcg gcctatagcc gatgcggagc gcaagtatca ctggccgctc
     1681 gggccccgcc cgcacggcca tcccggcttg tcagaactcg gggtgtgacg ccgacaccgg
     1741 cgtgactaac actgcgtcac ttccggcaaa acggccagaa ctggtaccac gaatcacatt
     1801 tgaagcgatc ttcgcactgt cgaagctgct cgtcatactc ccgcgcccgc cggtcgaccc
     1861 gttcggccag gttgatcagg tcgggcttcc ggcgccactc gccggatctg taaccggacc
     1921 ttccctcatg ataagcaagg tacaggttcc tggggtcccg gatcgatatg ccgagttcct
     1981 tgtgggactt gtggttgtac caaccgatga aatccagcgc gtccttcatg ttgtcccggt
     2041 ccgaaaacca agttgcgccg gtttccttca tgtagtcctt ccatgcagga tcctgggcct
     2101 gcgcgtagcc gtaagcggag gactgccggg gaagcggcac gatcctgagg aaccagtcgc
     2161 gcggcggc
//
LOCUS       DASUOM010000167         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_747106,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000167 DASUOM010000000
VERSION     DASUOM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747106"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFY27_03390"
     CDS             <1..879
                     /locus_tag="VFY27_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5764583.1"
                     /translation="EAGVPPGVFNLVNGDGATVGSALSSHPDIAMVSFTGSTRAGIAV
                     AQNAAPTVKRVAQELGGKSANIILDDADFEKAVARGASACFDNAGQSCNAPTRMLVPR
                     DRLEEAARIAAKRAEATTPGDPKDESTRIGPVVSETQWKKIQDLIQKGMDEGAALVAG
                     GIGRPAGLDRGWYVKPTVFSNVSNDMTIAREEIFGPVLSIIPYEDEDDAVRIANDTPY
                     GLSGYVTSSDLERARNVASRLRTGMVHINGAPLDALAPFGGYKHSGNGREWGARGLEE
                     FLEVKSIYGYEARKAG"
     gene            1005..>2081
                     /locus_tag="VFY27_03395"
     CDS             1005..>2081
                     /locus_tag="VFY27_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX5764584.1"
                     /translation="MTTARRSIAKIILPGEKFLRFFAVLAVAGIALGCTESQRPEASG
                     KGTIRGIHAAPTVGDATFLIEERPLGSLAYKASTTTQQFDDLSYSFHFDTRTPGQATQ
                     TRLASESLEVMRDMDYVFVLTGTFASPSILLWEAAERQWEGTETTFEVSAGHLSPGLG
                     DIDVYFADPDTAPAAGSTRGTLAFGERLPPFEVESGVYRLTITEAGNPAAVLFRAAPR
                     DHNERTSTLFTVQDADPSITSNLSVRRYDQNGNGAEVGDTSFPPTRRFYNAAFDSGSM
                     DVYVGDDFTTPIVTNLAFGTVSADVEVPAAESEYTYTAVGNPGAILAEDEDTVAANTR
                     GTNFVIGEPDELEVTVFADDRRPIT"
BASE COUNT          437 a          665 c          654 g          325 t
ORIGIN      
        1 gaggccggcg tcccgcccgg tgtgttcaac ctcgtcaacg gcgatggtgc cacggtgggt
       61 agtgcgttga gcagccatcc cgacattgcg atggtgtcgt tcaccggctc gacccgcgcc
      121 ggcattgccg tcgcgcagaa cgcggcgccg acggtcaagc gggtggcaca ggaactgggc
      181 ggcaagtccg cgaacatcat cctggacgat gctgacttcg agaaagcggt agcgcgcggc
      241 gcaagcgcat gcttcgacaa cgccggccag tcctgtaatg cgccgacgcg catgctggtg
      301 ccgagagacc ggctggaaga ggccgccagg attgccgcga aacgggccga ggcgacgaca
      361 cccggcgacc cgaaggacga atcgacgcgc atcgggcctg tcgtatccga aacacaatgg
      421 aaaaaaatcc aggacctcat ccagaagggc atggacgagg gcgccgccct ggtcgcaggc
      481 ggcatcggac gccccgccgg actggacagg ggctggtacg tcaaacccac cgtgttctca
      541 aatgtcagca acgacatgac catcgcgcgc gaggagatct tcgggccggt tctttccata
      601 attccgtacg aagacgagga cgacgccgtg cggatcgcga acgatacgcc ctatggtctt
      661 tccggctacg tcacctcgtc cgatctcgaa cgcgcacgta atgtcgcttc gcgcctgcgg
      721 accggcatgg tccacatcaa cggcgccccg ctcgacgcgc tggcaccttt tgggggctac
      781 aagcattcgg gcaacggccg cgaatgggga gcacgaggcc tggaggaatt tctcgaggtg
      841 aagtccatct acggctacga ggccaggaag gctggctgag acaacatcac cgccctaccc
      901 gttattgaac cccgaaggct cgagccggtc gcaagatcgg cagccggggc gcgcgcagtg
      961 cgaaaccgtc aggtttacga ccagagaaga gagcagcaaa aagcatgaca acggcaagac
     1021 gctcgatcgc aaaaatcatt ctcccggggg aaaagtttct gcgcttcttt gcagtactcg
     1081 cggtggcagg catagcgctc ggctgtaccg agagccagcg gccagaagcc agcggcaaag
     1141 gcaccatcag gggcatacat gcggccccga cggtgggcga cgcgacattc ctgatcgaag
     1201 aacgcccgct gggatcactg gcctataagg cttcaacaac gacgcagcag ttcgacgacc
     1261 tgagctatag cttccatttc gacactcgca cccccgggca ggcgacccag acaaggcttg
     1321 cgagcgaaag cctcgaggtc atgcgggaca tggactacgt gttcgtactg acgggcacgt
     1381 tcgcctcgcc gtcgatcctg ctttgggaag cggcggaacg acagtgggaa ggaacagaga
     1441 cgactttcga ggtctcggcc ggccacctgt cgccggggct cggcgacatc gacgtctact
     1501 tcgcggaccc ggacaccgcg ccggccgctg gcagcacccg cggaacactt gccttcggcg
     1561 aacgcttgcc gcctttcgag gtggagagcg gtgtctatcg gctgaccata accgaagcgg
     1621 gcaatccggc ggcagtcctg ttccgggccg ctccgcgcga ccacaacgaa cgcaccagca
     1681 ccctgttcac cgtccaggat gcggacccct ccattacgag caacctgagc gtgcggcgtt
     1741 acgaccagaa cggcaacggg gcggaggtcg gcgatacgtc ctttccgccc acgcgtcgct
     1801 tttacaacgc cgcgttcgac agcggcagca tggacgtcta cgtcggggat gacttcacta
     1861 caccgatcgt caccaacctg gccttcggca cggtcagcgc cgacgtagag gtacctgcgg
     1921 cggaatccga gtacacctac actgcagttg gcaaccccgg tgctatcctg gccgaggatg
     1981 aagatacggt ggcggccaat acacgcggca ccaacttcgt catcggcgag cccgacgaac
     2041 tggaggtcac cgtttttgcc gatgaccgcc ggccaatcac g
//
LOCUS       DASUOM010000168         5614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1339653,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000168 DASUOM010000000
VERSION     DASUOM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339653"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="VFY27_03400"
     CDS             complement(<1..1602)
                     /locus_tag="VFY27_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5764585.1"
                     /translation="MRLHPCKALLLTVLLAAGAPAMAQPVQQGDRNVPEFEPAFPEQT
                     RAPASDSGVTLSSETFAEPLEHPWGIDRLPGGGYLVTELPGRMRVISANGEISDPVEG
                     IPEVFYAGQGGLLDVAVGPTFEEDRMIYWSYARALEGGKSVTVASRGRLSPDQTRVTE
                     VEDIFAQEPPSPTPAHYGSRVLFDGEGHVFITTGDHFTEGERLLAQDLGTTYGKVIRL
                     NTDGSIPDDNPFIDEQGTIGSIWTLGHRNIQGIALDPGTSRLWTIEHGPQGGDELNLS
                     EPGSNYGWPTVSYGENYDGSPVGGGKERHEGKFVEPRYYWDPVIAPGGMLFYKGGMFA
                     EWRGDLLISSMVPGGIVRIELDGDTVTGEERLLKDHGRIRDIVEAPDGALLVLVDAAE
                     GAVLRLTPNQSGGAGAPREAWQAEGFHRPESVIFDNARNVLYVSNVGEDEEEGQGYIS
                     KLTRDGEVTEAQWVTGLKGPKGMAVYEDRLYVSDVDRLVAIDIEAGEVAESWDAPGSE
                     FLNDVAADGDGRVFVSDMNGNAIYAL"
     gene            1766..2914
                     /locus_tag="VFY27_03405"
     CDS             1766..2914
                     /locus_tag="VFY27_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5764586.1"
                     /translation="MNKAPDRILTTHTGSLPRPDDLIRMMWAVGDGIPVDKQALEKRV
                     ADAVKETVQKQLSAGISIINDGEMSKPSYATYVKDRLHGFGGATIQNYYFADLVDFPK
                     SAELVSANPGRRKRTAPACTGPISVRDPDAAVIDMRNLELAARACNAARTFSSAASPG
                     VVSIFFANEFYKSDEEYVFAIAEAMRHEYEAIAAAGATVQLDCPDLAMGRHSAYADMD
                     IAQFRRKVAMNIAALNHGVRNIPSEQLRMHLCWGNYPGPHHCDVAMSEIIDLVWTAKP
                     QTVLFEAANPRHAHEWTLFENTAVPDDKLICPGVIECQSNYIEHPELVAQRIERYANL
                     VGRERVMAGVDCGFSIHVGMQGIDPAVAWAKLAALSQGAEIATERLWR"
     gene            complement(2936..3301)
                     /locus_tag="VFY27_03410"
     CDS             complement(2936..3301)
                     /locus_tag="VFY27_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5764587.1"
                     /translation="MNTGGLRNWLRAYGEAWEGRDAEAASQLFSEDARYFETPYAEPF
                     RGRDGIRGYWAKVTDDQREVDFNSEALGFLGATGVARWSARFKLASNGVAVELNGVFL
                     LEFDENGLCSRLREWWHAR"
     gene            complement(3298..3867)
                     /locus_tag="VFY27_03415"
     CDS             complement(3298..3867)
                     /locus_tag="VFY27_03415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX5764588.1"
                     /translation="MHKVAVIVGSIRKDSHNAKLARALEKLAGDRLEFRRIRIDDLPL
                     LNEDHIDDPPGTVRRFKREVEECAAVLFVTPEHNRSVPAALKNAFDWGTRPWGWNSWL
                     GKSAAIIGTSAGGIGTALAQANLRTMASTHVAALLGTPEAYIQYKEGLFESDGTVTNE
                     ATREFLKSFIDNFVMLIEGTARSGQAGAK"
     gene            complement(3870..4916)
                     /locus_tag="VFY27_03420"
     CDS             complement(3870..4916)
                     /locus_tag="VFY27_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5764589.1"
                     /translation="MATERLPLFPCTIVGSYPQPHWLIDRQKLSKQFPPRTRAAELWR
                     IEPALLADAQDDATRLAILDQERAGLDIVSDGEIRRESYSNHFATALSGIDLNNPGKA
                     LDRSGEPVFVPRITGAIRRRHPVGVEDVKFLRDNTSRSIKFTVPGPFTMTQQAQNDFY
                     ETEAEAALAYAAAVNEEIRDLFEAGADVVQLDEPYMQARPEKAREYGLAALDRALEGI
                     AGKTCVHICFGYAALIHERPEGYSFLPELGNASCSQVSIETGQSGLDCSVLESLSGKD
                     ILLGVLDLSSNEVETVETIKSRVRRGLEHVPAEHILLAPDCGMKYLSRKAAFGKLSAM
                     VQAARELREEYGGD"
     gene            complement(4922..>5614)
                     /locus_tag="VFY27_03425"
     CDS             complement(4922..>5614)
                     /locus_tag="VFY27_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764590.1"
                     /translation="EPGGGDPLIRERIEDAFGCKVRESMGIGDISLSAWAEDDDGHGM
                     HFMARGFVHVELIDPESGESRSWEHRAEGELVYTALARDAMPLLRFRSRDHVVVNMEP
                     NPLGRTGPRIRCIGRTDDMLIVRGVNLFPSALRSILKEFMPYVSGMLQVRPKSEGVLQ
                     SPPLPVVVELGKSCLTPPETLRQGIVDEIRSRLLVTTNVELVPYGTLPRETYKSKLID
                     YSDVEPPKTASG"
BASE COUNT         1035 a         1820 c         1682 g         1077 t
ORIGIN      
        1 gagcgcatag atcgcgttgc cgttcatgtc ggatacgaag acgcgcccgt caccgtccgc
       61 ggcaacgtca ttcaggaact cggacccggg tgcatcccag ctttcagcaa cctcgcctgc
      121 ctcgatgtcg attgcgacca accggtccac gtcggagacg tagaggcggt cttcgtaaac
      181 cgccataccc ttcggtcctt tgaggccggt tacccactgc gcctcggtta cttcaccatc
      241 gcgggtcagc ttcgagatgt aaccttgtcc ttcttcttcg tcttcgccca cgttcgatac
      301 gtaaagtacg ttgcgggcgt tgtcgaagat caccgattcc ggccggtgga atccttcagc
      361 ctgccatgct tcccgggggg cgcccgcgcc cccggactgg ttcggcgtca gtcgcaggac
      421 agcgccttcg gccgcgtcga cgaggacgag cagcgcgcca tcgggagctt cgacgatgtc
      481 gcgaatacgc ccgtgatcct tgagcaggcg ctcttcgccg gtcacggtat cgccgtcgag
      541 ttcgatgcgc acgataccac ccggcaccat cgaggaaatg aggagatctc cccgccactc
      601 agcaaacatg ccgcctttgt agaagagcat gccgcccggc gcgatcaccg ggtcccagta
      661 ataacggggc tcgacgaact tgccttcatg gcgctcctta ccaccaccta ccgggctccc
      721 gtcgtagttc tccccgtaac tcacggtagg ccagccgtag ttcgatcccg gctcactcag
      781 gttcagctca tcaccgccct gcgggccatg ctcgatcgtc cacagccgac tggtgccggg
      841 atcaagggcg atgccctgga tgttccggtg gcctagcgtc cagatggagc cgatggtccc
      901 ttgctcgtcg atgaagggat tgtcgtcggg gatggagccg tcagtattga ggcggatcac
      961 cttcccgtag gtagtgccga ggtcctgggc gagcaggcgc tcgccttcgg tgaaatggtc
     1021 tcccgtcgtg ataaaaacgt ggccttcgcc gtcgaagagg acgcgtgaac cgtagtgcgc
     1081 aggcgtcggc gacggcggct cctgcgcgaa gatatcctcg acttcggtca ccctggtttg
     1141 atccggggag agccggccgc ggcttgcgac agtcactgac ttgccgcctt cgagcgctct
     1201 cgcgtaactc cagtagatca tccggtcttc ttcgaacgtc gggccgacgg ctacgtcgag
     1261 caagccgcct tgcccggcgt aaaacacttc cggaatgcct tctacgggat cggagatttc
     1321 tccgtttgcg gagattaccc gcatgcgacc gggaagttcc gtgaccaggt atccgccgcc
     1381 aggcaggcgg tcgatccccc acggatgttc gagaggctcg gcgaatgtct cggacgagag
     1441 ggtaacgccg gaatccgacg caggtgcgcg cgtctgttcg gggaaagccg gttcgaactc
     1501 aggtacgttg cgatcgccct gctgcaccgg ctgcgccatg gccggcgcgc cggcggcgag
     1561 tagcacagta agaagcaacg ccttgcaggg atggagcctc atggcaacct ccaggtggaa
     1621 tgtgagtttt agggaattgc cagcggggac tttggtcgct tcggttcctt gcacacaccg
     1681 gcaaggctgg caactggtac cgacgcgcgt acgacggata acatagtgcc acactgccgg
     1741 cgaaagcgcc tgtacgggaa actacatgaa caaagcaccc gatcgaatcc tgaccacgca
     1801 cacgggcagc ctgccaaggc cggacgacct catccggatg atgtgggcag tgggcgacgg
     1861 cataccggta gataaacagg cgctcgaaaa gcgggtcgct gacgccgtga aggagacggt
     1921 gcagaagcag ctgtccgccg gcatcagcat catcaacgac ggcgagatgt cgaagcccag
     1981 ttatgccacg tacgtgaagg atcgccttca cgggtttggc ggcgcgacga tccagaacta
     2041 ctacttcgcg gacctcgtgg atttcccgaa gagcgccgaa ctcgtttccg ccaatcccgg
     2101 gcgacggaag cgcacggcac ccgcctgcac cggacccatc tcggtcaggg atccggacgc
     2161 cgccgtaatt gacatgcgaa atctggaact cgccgcacgg gcttgcaatg cggctcgcac
     2221 attctcgagc gcggcgtcgc ccggagtcgt ttcaatcttc tttgccaacg agttctacaa
     2281 gagcgacgaa gagtatgtct tcgccatcgc cgaagccatg cggcacgaat atgaagcgat
     2341 cgccgcggca ggcgccacgg tgcaactcga ttgcccggac ctggcgatgg gtcgccattc
     2401 cgcgtacgcc gatatggaca ttgcacagtt tcgcaggaag gtagcaatga acatcgcggc
     2461 gttgaaccac ggcgtacgca acattccttc agaacaactg cgcatgcacc tctgctgggg
     2521 gaactacccg ggtccgcacc attgcgatgt ggccatgtcc gagatcatcg acctggtgtg
     2581 gactgcgaag ccgcagacgg tgttgttcga ggcggccaac ccgcggcatg cgcacgagtg
     2641 gacgttgttc gaaaacaccg ccgtcccgga cgacaagctc atctgcccgg gcgtcatcga
     2701 atgccagtcg aattacatcg agcatccgga gctggtcgca cagcgaatcg agcgttatgc
     2761 gaatctcgtc ggccgcgagc gcgtcatggc cggcgtcgac tgcggattca gtatccacgt
     2821 cggcatgcag gggattgatc cagcggtggc ctgggccaag ctcgccgccc tgtcacaggg
     2881 agcggagatc gcgacggaac ggctctggcg ctagatgggg tgcaccaggt cgccctcagc
     2941 gtgcgtgcca ccactcgcga agacggctgc aaaggccatt ctcgtcgaac tccagcagga
     3001 acacgccgtt caactcgaca gcgacgccgt ttgatgccag cttgaaccgc gccgaccacc
     3061 tggccacccc cgtcgctccg aggaatccca gcgcctcgct gttgaagtcg acttcccgct
     3121 ggtcatcggt caccttcgcc cagtaacccc tgatgccgtc ccggccacgg aagggctccg
     3181 cgtacggcgt ttcgaagtaa cgagcgtcct cgctgaagag ttgcgaagcc gcctccgcat
     3241 ccctcccttc ccaggcttcg ccataggcgc gcagccagtt gcgcaggcca ccggtattca
     3301 tttcgcgcct gcctggcctg agcgagcggt gccttcgatc agcatgacga aattgtcgat
     3361 aaagctcttc aggaactccc gcgtcgcttc gttcgtcacc gtgccatcgc tttcgaaaag
     3421 accttccttg tactggatgt aggcttcggg cgtccccagc agcgcggcaa cgtgagtact
     3481 ggccatggtg cgcaggttgg cctgcgcgag tgccgtgccg ataccgccgg ccgatgtgcc
     3541 gatgatcgcg gccgacttgc cgagccagga gttccagccc catggccgcg ttccccagtc
     3601 gaaggcattc ttcagcgcag caggcacgga ccggttgtgc tcgggcgtga cgaatagcac
     3661 ggccgcacac tcctctacct cacgcttgaa gcgccgtacc gtccccggtg gatcatctat
     3721 gtgatcctcg ttgagcaagg ggaggtcgtc gatacggatc ctgcgaaact cgagcctgtc
     3781 gcccgccagc ttctcgagcg cccgcgccag cttggcgttg tgggaatcct tgcgaatact
     3841 gccgacgatc acggcaactt tgtgcatcat caatctcctc cgtactcttc acgcagttcc
     3901 cgcgccgcct gcaccattgc cgagagcttg ccgaacgcgg ctttcctcga gagatacttc
     3961 atgccgcagt cgggtgcaag caggatgtgc tccgccggca cgtgctcgag accccgcctg
     4021 accctggact tgatcgtctc caccgtttcg acctcgttgc tcgagaggtc cagaactccg
     4081 agcaggatgt ctttgccgga aagtgactcg agcacgctgc agtcgagacc cgactgcccg
     4141 gtctcgatag atacctggct gcaagaagca ttgccgagtt ccgggagaaa cgaataccct
     4201 tccggccgct cgtgtatcaa tgccgcgtaa ccgaagcaga tgtgcacgca ggtcttgcca
     4261 gcgatgcctt ccagggcgcg atccagcgcc gcgaggccat actccctcgc cttctccggc
     4321 cgcgcctgca tgtaaggttc gtccagttga accacgtcgg cgccggcttc gaacaggtcg
     4381 cgtatctctt cgttgacggc ggcggcataa gccagcgccg cctccgcctc cgtttcatag
     4441 aagtcgtttt gtgcctgctg ggtcatcgtg aacgggccgg gtaccgtgaa cttgatgctg
     4501 cggctcgtgt tgtcgcgcag gaacttcacg tcctcgacgc cgaccgggtg gcgccggcgt
     4561 atggctcccg tgatcctcgg cacgaacacg ggctcgccgc ttcgatccag cgctttgccg
     4621 gggttgttca ggtcgattcc ggagagtgct gtcgcaaagt ggttggaata actctcccgg
     4681 cggatctctc cgtcggagac gatgtcgagg ccggcccgtt cctggtccaa aatcgccagc
     4741 cgggtcgcgt catcctgcgc atccgcaagt aatgctggtt cgatccgcca cagttctgcc
     4801 gcgcgcgtgc gtggcgggaa ctgcttcgac agtttctgcc gatcgatcag ccagtgcggt
     4861 tgcggatagc tgccgacgat cgtgcaggga aacaggggca atctctcagt ggccattcgt
     4921 atcaacccga agccgtcttt ggcggctcca catccgaata atcgatcagc ttcgacttat
     4981 acgtttcacg gggaagcgtg ccgtaaggca cgagttcgac attggtggtc accagtagcc
     5041 gtgagcgtat ctcatccacg atgccttgcc gcaacgtctc cggaggcgtc aggcagctct
     5101 tgcccagctc caccacgacc ggcagcggcg gggattgcag gacgccctcc gacttcggcc
     5161 ttacctgcag cattccggat acataaggca tgaactcctt cagaatgctt cgcagcgcgg
     5221 aggggaacag gtttaccccg cgcacaatca gcatatcgtc ggtgcgcccg atgcagcgga
     5281 tacgcgggcc ggtgcgtccc aaagggttcg gctccatgtt caccaccacg tgatcgcggc
     5341 tcctgaagcg cagcaacggc atggcatcgc gcgcgagcgc cgtatacacc agctctccct
     5401 cggcccggtg ctcccaggat ctcgactcgc cgctttcggg atcgatcaac tcgacgtgca
     5461 cgaagccccg cgccatgaag tgcatgccat gcccgtcgtc gtcttcggcc caggcggaca
     5521 aggatatgtc gccaatcccc atggattcgc gcaccttgca gccgaacgcg tcctcgatac
     5581 gctcgcggat caacgggtct cccccgcccg gctc
//
LOCUS       DASUOM010000169         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1554655,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000169 DASUOM010000000
VERSION     DASUOM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554655"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFY27_03430"
     CDS             complement(<1..434)
                     /locus_tag="VFY27_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764591.1"
                     /translation="MNKPATHSFLMAATGTLLLLAGQVYATDELPAGQAAPTVEEQSE
                     PQVLPAPHVAEDVTYPQPAASQQAETTAHEEPAAEVEVPANDHPGQSAVPAVADDAAE
                     DVAQRVPETGEIVQGQAAVESVLEPETSGTVQVDSGLVAGLA"
     gene            579..2519
                     /locus_tag="VFY27_03435"
     CDS             579..2519
                     /locus_tag="VFY27_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5764592.1"
                     /translation="MTSTNRRISLLLLLPFVCVAFEASADEWFPRPPELEPDVSFWVS
                     IFTEYDSNQGVLHDNRNLSIVYERVDISAKVSRTKRNNTVALRRAHYAKVLQTLAGGK
                     RDNLSREEQRVLRLWPEGVSNSELADAVDNIRYQQGLSDRFREGLRRAGRYRAHVHRE
                     FARLGVPVELAALPHVESSYNPDARSHVGASGIWQFTRSTGRRFMQVDHVLDERNDPF
                     AATVAAGRLLAYNHSITGNWPMAITAYNHGLSGVRRAMQAHGDTAYVEILREYRGRAF
                     GFASRNFYVAFLAALQVDRNAGKYFPGLEPYPPVDYETIVLDAYLPADSVAGTLGISQ
                     AELARHNPALQPTVWQGSKHLPRQYQLRLPRGLLKAPLTELMASLPGDAHYAEQLPDM
                     FHTIAPGDTLSEIAREYNTGVSTLVALNNLGSSNRIRAGQQLRLPAAGPLPVMVASTS
                     EAADVVTAAPEPAVVAVASVPDEEFPEIEPEGGALADDLPENAEGVVQLSLLSDPNDY
                     TVAGDRTIEVHPLETLGHFADWLGVRTQRLRDINGLAFRTPVEVGQRIELDLSRVDAA
                     SFESLRAAWHKEQQDAFFRDHRITGVHEHVVKNGEAVWVLALQRYNVPVWLLRQYNPE
                     LDLHKVRKGVRLNFPVLASNSG"
     gene            2550..2942
                     /locus_tag="VFY27_03440"
     CDS             2550..2942
                     /locus_tag="VFY27_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764593.1"
                     /translation="MPAILFRASPVIVAVAAAMAYVVYVEWPGPYVAMNVLPMLVVIV
                     LAFICLYRGGGRWSGSGFKWPLGTLGFALSAIGVSLYLHYGYATDRDGMVSEATTPVE
                     LFRYLPVYALFAGATGFALGWIAGRNLR"
     gene            3046..3654
                     /locus_tag="VFY27_03445"
     CDS             3046..3654
                     /locus_tag="VFY27_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5764594.1"
                     /translation="MSRTSMEQASANAWLALAFVTLTLAFSTASADSDGGSKNGGSSI
                     SSVTRVGTQYDPYDQGFPLADRVVVRKADRKLELIKDGETFRTFDVALGIAPYGHKEE
                     EGDNRTPEGTYWLDMRNPNSDYFLSIRISYPDENDRRLARQKGVDPGGQIMIHGQPNK
                     PNYSAAYYRQADWTNGCIAVSNSDMIDIWLMTPDRVAIEILP"
     gene            3720..>4742
                     /gene="ppnN"
                     /locus_tag="VFY27_03450"
     CDS             3720..>4742
                     /gene="ppnN"
                     /locus_tag="VFY27_03450"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEX5764595.1"
                     /translation="MSRRKAAIRAESDQTVDAEVWPEGSLEVLSHSEAEQLRNQGEGG
                     LYPLLRRCLLAVLNSGNETDDARGVLQRYQDFRIGFAQQDRGLKVLLKGAPSSAFVDN
                     QMIRGIRELVFAVLRDIVYTNNEIRESNLFHLKTSENITDAVFHIARNARLLQLPATP
                     NLVVCWGGHAISRDEYDYTKQVGYELGLRGLNICTGCGPGAMKGPMKGATIGHAKQRI
                     RNGRYIGITEPGIIAAESPNPIVNSLVIMPDMEKRLEAFVRIGHGIVIFPGGVGTAEE
                     VLYLLGILLHPNNERLPFPVIMTGPPASEPYFRQIEEFIATTLGAEAERRYSIIIGDP
                     EDVARKM"
BASE COUNT          910 a         1464 c         1457 g          911 t
ORIGIN      
        1 atggcgaggc cggcgaccaa cccgctgtcg acctggacgg tgccggacgt ttccggctcg
       61 agtacgcttt caacggcggc ttgaccttgg acaatctcgc ccgtctccgg gacgcgctgc
      121 gccacgtctt cggctgcgtc gtcggcgact gcgggcaccg cggattgacc ggggtggtca
      181 ttagcaggaa cttccacttc cgccgccggt tcttcgtgcg ctgtggtctc ggcttgttgc
      241 gaggcagccg gttgcgggta agtgacgtct tccgcgacat gcggcgctgg cagcacttga
      301 ggttccgatt gctcctccac cgtcggcgca gcctgcccgg ccggaagctc gtcggttgcg
      361 taaacctgcc cggccagcaa caagagggtg ccggttgcag ccatcaggaa tgagtgtgtc
      421 gcgggtttat tcatcggctt ttttattgca gggaatcggc tgattcgggt tgcggttcac
      481 agcggcagag gtaagaatgg cttataaaga gcttgggttc tgtgaaacta ttcaagcgtt
      541 tagaattaac tcctcgattg aagcctgatt ctgcctaaat gacaagtaca aatcgcagga
      601 tttcactgct actgctgctg ccgttcgtct gcgttgcgtt tgaggcaagc gccgatgagt
      661 ggttcccgcg ccccccggag ctcgaacccg acgtcagttt ctgggtctcg atcttcacgg
      721 aatacgacag taaccaaggc gtccttcacg ataaccggaa cttgagcatt gtctatgaac
      781 gggtcgacat ttcggcgaaa gtctcccgga caaagcgcaa caacaccgta gctctgcggc
      841 gtgcccacta cgcaaaggtc ctgcagacgc tggcaggcgg caagcgcgac aacctgtcgc
      901 gcgaggaaca gagggttttg cgcctctggc ccgagggtgt gtccaacagc gagcttgcgg
      961 atgcggtcga taacatccgt taccagcagg gcctctccga ccggttccgg gaagggttga
     1021 ggcgcgccgg gcgctatcgc gcccacgtgc accgtgagtt cgcgaggctg ggcgtgccgg
     1081 tggaactggc ggcgctgcct cacgtggagt cgtcctacaa cccggatgcc cgttcccatg
     1141 tcggcgcctc cggtatctgg cagtttaccc gcagcaccgg acgccgcttc atgcaggtcg
     1201 accatgtgct cgacgagaga aacgaccctt ttgcggcgac cgttgccgcc ggcaggctcc
     1261 ttgcctacaa ccactcgatc accggaaact ggccgatggc gatcaccgcc tacaaccacg
     1321 gcctctccgg cgtccggcgc gccatgcagg cgcacggcga cacggcctat gtcgagattc
     1381 tgcgggagta ccgcggcagg gctttcggct tcgcttcccg caacttctat gtcgcgttcc
     1441 tggcggccct gcaggtcgac cggaacgccg ggaagtactt cccgggcctc gaaccgtacc
     1501 cgccggtgga ctatgaaacg atcgtcctcg atgcctacct tccggccgat tctgttgcgg
     1561 ggacgttggg catcagccag gcggaactgg cacgccacaa tccggctttg cagccgaccg
     1621 tctggcaggg aagcaaacac ctgccgcggc agtaccagct tcgcctgcct cgcggcctgc
     1681 tcaaggcgcc cctgacggag ctgatggcat cgttgccggg cgatgctcac tacgcggagc
     1741 agcttccgga catgttccac accattgcgc cgggcgacac gctctcggag attgcccgcg
     1801 agtacaacac cggcgtatcc acgctggtcg cactgaataa cctcggcagc agcaaccgga
     1861 tacgggccgg gcagcaattg cgcctgcctg ccgccggacc gctcccggtc atggtcgcga
     1921 gcacatcgga ggcggcagat gtggtaacgg cggcgccgga accggctgtg gtcgcggttg
     1981 cctccgttcc cgacgaggaa ttcccggaaa tcgaaccgga gggcggcgcc ctcgccgatg
     2041 acctgccgga gaacgcggag ggggtcgtgc aactttcgct gctctcggac cccaacgatt
     2101 atacggtcgc gggggatcgc acgatcgaag tacatcctct cgagacgctc ggacatttcg
     2161 cggactggct gggcgtcagg acacagcgct tgcgcgacat caacggactc gcgttccgta
     2221 cgccggtcga agttggacag cgcatagaac tggacctgag ccgcgttgac gccgccagct
     2281 ttgaatcgct ccgtgccgcc tggcacaagg agcagcagga tgcattcttc cgcgaccatc
     2341 ggataaccgg agtgcatgag cacgtcgtga agaacggcga agcagtctgg gtgctcgccc
     2401 tgcagcgcta caacgttccc gtctggctgc tccgccagta caacccggag ctggacctgc
     2461 acaaggtacg gaagggcgta cgcctcaatt tcccggtgct cgcgagcaac agcggctgat
     2521 cttcgaacac tgtacgcgtg aagcccgggt tgcctgcgat cttgttccgt gcatcgcccg
     2581 tgatagtggc tgtcgccgcg gcgatggcgt acgttgtgta cgtcgaatgg ccggggccat
     2641 acgttgcgat gaacgtcctt ccaatgctcg tcgtgatagt gctggcattc atatgccttt
     2701 accggggcgg cggccgatgg agcggttcgg gcttcaagtg gcctctcggc acactcggct
     2761 tcgcgctgtc cgccataggc gtatcgctct acctgcatta cggatacgcc accgatcgcg
     2821 acgggatggt gagcgaggca acaacgcccg tggaattgtt ccgctacctg ccggtatacg
     2881 cgctgtttgc aggagccact ggattcgccc tcggctggat cgctgggcgt aatcttcgct
     2941 gatttcggtc cggacttggg cagcggctcg ccgggtcaga ttctcaaatt gtgccgtgtc
     3001 tgctagtctg atcgggtgta ccgcgagata ttcaggccta tgccaatgag ccgtacgtcc
     3061 atggagcaag cctctgccaa tgcctggctg gcacttgcct tcgtgacgct taccctcgca
     3121 ttctccacgg cctcggcgga cagcgatgga ggcagcaaga acggcggcag cagcatttca
     3181 tcagttactc gtgtcggcac gcaatacgac ccgtacgacc agggctttcc gcttgcggac
     3241 cgggtcgtcg tgaggaaggc agaccggaaa ctcgagctca tcaaagacgg tgaaaccttt
     3301 cgcaccttcg acgtcgccct gggaatagcc ccttacggtc acaaggagga ggagggcgac
     3361 aacaggaccc cggagggaac ttactggctg gacatgcgaa acccgaacag cgactacttc
     3421 ctgtcgatac gcatatcgta ccctgacgaa aacgaccggc gccttgcacg gcagaagggc
     3481 gtggaccccg gcggccagat catgattcac gggcagccca acaagcccaa ttactcggcg
     3541 gcctactacc gccaggccga ctggaccaac ggctgcattg cagtctccaa ctccgacatg
     3601 atcgacatct ggctcatgac ccccgatcgc gtcgcgatcg aaattttgcc ttagtcccac
     3661 aggctggact tttccccgct tcagcctgca catccaggaa gagacatttg cacgaagcat
     3721 tgagtcgacg gaaagcggcc atccgggccg agagcgacca gacggtcgat gccgaagtat
     3781 ggccggaggg cagcctggag gttctgtcgc attcggaagc cgagcagctg agaaaccagg
     3841 gggaaggcgg cctgtacccg cttcttcgcc gctgcctgct ggcggtactc aattccggta
     3901 acgagacgga tgatgcccgc ggcgtgctgc agcgctacca ggatttcagg atcggtttcg
     3961 cgcagcagga ccgggggctc aaagttctgc tgaaaggcgc tccgtcgtct gcattcgtcg
     4021 acaaccagat gatccgcggc attcgcgaac tggtgttcgc ggtgctgaga gatatcgttt
     4081 acaccaataa cgagattcgc gaaagcaacc tctttcatct caagacgtcg gagaacatca
     4141 ccgacgccgt tttccacatc gcaagaaacg cgcgactgct gcagttgccg gcgaccccaa
     4201 atctcgtggt ctgctggggc ggacacgcca tcagccgtga cgaatacgac tacaccaagc
     4261 aggtgggcta cgaactggga ctgaggggtt tgaatatctg taccggttgc ggacccggtg
     4321 cgatgaaagg tccgatgaag ggggctacca tcggccatgc gaaacaacgc atccgcaacg
     4381 gtcgttacat aggcattacc gagccgggta tcatcgctgc cgaatcaccg aacccgatcg
     4441 tcaattcgct cgtgatcatg ccggatatgg aaaagcgcct ggaggcattc gtccgcatcg
     4501 ggcacggcat cgtgattttc ccgggcggcg tcgggacggc ggaggaggtc ctctacctgc
     4561 tgggcattct cctgcacccg aacaatgagc gcctgccgtt tccggtcatc atgaccggcc
     4621 cgcccgccag cgaaccgtat ttcaggcaga tcgaggaatt tatcgcgacc acgcttggcg
     4681 ccgaggccga acgccgatac tcgataatca tcggcgaccc ggaagatgtg gcgcgcaaga
     4741 tg
//
LOCUS       DASUOM010000170         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1627775,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000170 DASUOM010000000
VERSION     DASUOM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627775"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..>2956
                     /locus_tag="VFY27_03455"
     CDS             154..>2956
                     /locus_tag="VFY27_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5764596.1"
                     /translation="MPKRLVLRKLMDQVAGATSLQDVYDGALTCLHETLHLKRAAVLV
                     FDQDHVMRFVASSGLSDSYRQAVEGHSPWSSDNRAPQPVLVSDVRSAPEFASFLPLFE
                     QEQIAAVAFIPLCFGNKLLGKFMLYYRRPHEFADDELAVAETIAGHVAFAVEHHRIAS
                     QLEAKLATEHRLLSKAELDVAARQQNEQRLQMALAAGNMCAWEWDIATGEIQWSEELH
                     DMFGLDAAAFSGSLEAYQQHIHPVDRPRVEADIARMIRNRQSTYDLEYRLLPPDGALR
                     WVASRGRAVFDNDGETVRMVGVCTDITERKRLELSKEFVAEASRILATTLRPEDTIKK
                     LAQLVVPRLADWCIIQVVDEHGEIKPVELAHTNPENVELAWRVARRWQVPSQDGSASA
                     AIRTGESTLVAHIEPHMIAARAIDAEHLRVLESLELRSAITVPLKARGRKLGALTLLT
                     AESRRTYDATDLRFAEEIGSWAALAIDTAQLNREAENARSVAERSREYLQILARVSDD
                     LASSLDPDSALQQLASRLVGNLADYCVTYASDGVTIRRLGLAHRDPAKLARVEALCEA
                     GPPRFEDKWGAGAVIRTGEAILVEDIPEKLLDERARNERHRQAARQLAPVSAIIVPLR
                     ARGRTLGAIALVTDATSNRHYDHDDLKLARELASRAALLVDNARLYAQARAAVRARDD
                     MIAVVSHDLRSPLQSISSAATLLEFESQGGSANRSLQAIKLATSQMDRLLQDLLDLSL
                     IDAGQLTVTRELADAATLVDEARTMFEPLAGDKSVRLECKIQPGLPQIRVDRNRMQQV
                     LANLLGNALKFVPAGGLISLSANVQGDQLRIAVSDTGPGISEDDLPKVFDRFWRADRR
                     KERGVGLGLTVAQGIVQAHGGTIGVESRKGKGSTFYILLDAVQPVRKTAVPLGQKEGP
                     VLVVDDDEPFRTEIVEALQR"
BASE COUNT          588 a          924 c          943 g          501 t
ORIGIN      
        1 tcatagcact tcgaagatga tgccggttcg cgcctgccgt accgggcgcg gcggaagaag
       61 tttgcaatgt ctcccgcagg cgacgctagg attttcaaaa atacagtgtt aaaaatatcg
      121 accctcacgc ttaaaacgag aaaggagcga ttggtgccaa agaggctggt gctccgcaag
      181 ctgatggacc aggtcgctgg tgccacgtcc ttgcaggacg tgtatgacgg cgccctcacc
      241 tgcctgcacg aaacgctcca tttgaagcgc gccgcggtac tggttttcga ccaggaccat
      301 gtaatgcgct ttgttgcctc cagcgggctc tccgacagct atcgccaagc cgtggaaggg
      361 cattcgccct ggtcgagtga taacagggct ccgcaaccgg tactcgtgag cgacgtgcgt
      421 agcgcccccg agttcgcatc cttcctgcca ctcttcgaac aggaacagat tgcggcagtt
      481 gctttcatcc cgctttgttt cggcaacaag ctgctgggca aattcatgct ttactaccgg
      541 cggcctcatg aatttgccga cgatgaactg gcggtagcag agacgatcgc cggccatgtc
      601 gcattcgccg tggaacatca ccggatagcc tcgcagctcg aggcgaagct tgctaccgag
      661 caccggctgc tgtcgaaagc ggaactcgac gtagctgcga ggcagcaaaa cgagcaacga
      721 ctgcagatgg cgctggctgc gggcaacatg tgcgcgtggg aatgggatat cgcgaccggg
      781 gagatccagt ggtccgagga actacatgat atgttcggcc tcgacgccgc ggcgttctct
      841 ggatcactgg aggcctacca gcagcatata catcccgtgg atcgtccccg cgtcgaggcc
      901 gacatcgccc gcatgatccg caaccgccag tccacctacg acctcgaata ccgcctcctt
      961 cctccggacg gagcgctgcg ttgggtggcg tcgcgcgggc gcgcagtctt cgacaatgac
     1021 ggcgagaccg tgcgaatggt cggagtctgc accgacatca cggagcgcaa gcggctggag
     1081 ctgtccaagg aattcgtagc cgaggccagc cggatactcg ccaccacgtt gcggcccgag
     1141 gacaccatca agaaactggc ccagctggtc gtgccgcgtc tcgcggactg gtgcatcatc
     1201 caggtcgtcg atgagcatgg cgaaatcaaa cccgtcgaac ttgcgcacac caacccggaa
     1261 aacgtcgagc tggcatggcg ggtagcgcgg cgctggcagg ttccgtcaca ggacggctcg
     1321 gcctcagccg ccatacggac cggggaatcg acgctggtag ctcatatcga gccccacatg
     1381 atcgcggccc gcgctatcga cgccgagcac ttgcgcgtgc tcgagtcgct ggagctgcgg
     1441 tccgccatca ccgttccact gaaagcgcgc ggccggaagc tgggcgcgct gacgctactg
     1501 acggcggagt cgcggcggac gtacgacgcg acggacctgc ggttcgcgga ggaaatcggc
     1561 agttgggcag cccttgcgat cgataccgcg cagctcaatc gtgaagcgga aaacgcgcgg
     1621 agcgtcgccg agcgatcgcg agaatacctg cagatccttg cccgcgtgag cgatgacctc
     1681 gcgtcttcgc tggacccgga ctcggcgctg caacagctcg cgagccgcct ggtgggtaac
     1741 cttgccgact actgcgttac ctacgcgagc gacggcgtga ccatccggag actgggactg
     1801 gcgcatcgcg accccgcaaa gctcgcccgt gtcgaggctt tgtgcgaggc ggggccgccg
     1861 aggttcgagg acaagtgggg ggcaggcgcc gtcattcgta ccggcgaggc aattctcgtt
     1921 gaagatattc ccgagaagct gctggatgag agggcccgga acgaacggca ccggcaggcg
     1981 gccaggcagc tcgctccggt ctctgccatt atcgtgcccc ttcgcgcgcg cggccggaca
     2041 ctcggtgcaa ttgcgctggt cacggacgcg acttcgaacc ggcactacga tcacgacgat
     2101 ttgaagctcg cgcgggagct cgcgagtcgc gcggctctgc tggtcgacaa cgccaggctt
     2161 tatgcccagg cgcgagcggc agttcgcgca cgagacgaca tgattgccgt tgtttcgcac
     2221 gacttgcgga gccctttaca gtcaatctcc agcgcggcaa ccctgctgga gttcgaaagc
     2281 cagggcggct cggccaaccg cagcttgcag gcaatcaaac tcgccacatc gcagatggac
     2341 aggctcttgc aggacctgct cgacctgtca ctgatcgatg ccgggcagct caccgtaacg
     2401 cgggaactcg cggatgcggc cacgctggtc gacgaagcac gaacgatgtt tgagccgctc
     2461 gccggagaca aatccgtccg gctcgaatgc aaaatccagc ccggcctccc ccagatacgt
     2521 gtcgatcgca accgcatgca gcaggtactg gcgaacctgc tgggcaacgc gctcaagttc
     2581 gtgccggcag gcggccttat ctccctcagt gcgaatgtgc agggagacca gctgcgtatc
     2641 gccgtgtcgg acaccggtcc aggcatctcg gaggacgatc tgccgaaagt attcgaccgg
     2701 ttctggagag ccgaccgacg caaggagcgc ggcgtaggcc tgggactgac cgtcgcccag
     2761 ggcatcgtgc aggcgcacgg cgggacgatc ggcgtcgaga gccgcaaggg caagggaagc
     2821 acgttctaca tcctgctgga cgcggtccaa cccgtacgaa agaccgcggt tcctctcgga
     2881 cagaaggaag ggccagtgct ggtggtggac gacgacgaac ccttccgcac ggaaatcgtc
     2941 gaggcgctgc aacgct
//
LOCUS       DASUOM010000171         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1593402,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000171 DASUOM010000000
VERSION     DASUOM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593402"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFY27_03460"
     CDS             <1..932
                     /locus_tag="VFY27_03460"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5764597.1"
                     /translation="FMIQGLLAARQYFDEDTAIEREIRSTITRLWREVEWNWYRKTPD
                     GEVLYWHWSPEHAFHINHPLIGWNETMIVYLLAVASPTHAVPAELYHTGWAGTSDLHT
                     WYRQGWGRTKDGNRYVNGNTYYGKKLDVGVGAGGDLFFAHFSYMGFDPRGIRDRSTNY
                     FDNNRAIALINHAYCVENPLGFEGYGPDTWGLSAGVYTGGGRPQPRDDNGTINVMASL
                     ASMPYTPEKSMAALKHYYRDLGGKVWGAYGFHDGFNATENWFDEVYMALNQAPITVMI
                     ENHRSGLVWKQFMANPEIKPALDAIGFVDDEAP"
     gene            1065..3239
                     /locus_tag="VFY27_03465"
     CDS             1065..3239
                     /locus_tag="VFY27_03465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5764598.1"
                     /translation="MVLIASCGAVLGCTDNPNGDTRQSGNAPPVEAPVEEGDMQNPLL
                     AEWTGPYGGLPAFDKMHLEHLKPALEQAMEQRLREIDSIASNGEPPTFENTILALQRE
                     GKALDRVRTYYGLWRSNLSTPEFRGIQEEMEPRLAAHESAITQNDALFDRVRAVYESH
                     ELKMRTPDEQRLVRLIYDDFARNGATLEGEAKARYAEIDQRLSELHTRFANNVLADEE
                     KYVLCIRGNQLGGLPASFVQAARAAGEERCAAGEYAITNTRSSMDPFLTWSDQRDLRE
                     QVWRTYYNRGDNDDENDNNAIIAEILKLRDERVGLLGYDNYAQWRLEDRMAKTPENAL
                     ALLRAVLPAALARVDEEIADMQAIADERGDGIEIEAWDYRYYAELVRRDRYALDSDQV
                     KQYLQLDKLRDAMFFVAGELFDFEFKPVPEGSVPVFHPDVNVWEVTDRTSGEHVGLWY
                     LDPFARPGKRSGAWASTYRGHSTFDGRETVLSSNNSNFIKGAPGEPVLISWDDATTYF
                     HEFGHALHALSSNVRYPTLNSGVRDYTEFHSQLLERWLPTEPVVGNYLVHFETGEPIP
                     GELVAKIENAATFNQGFETTEYLASALVDMMYHTTDPAEIDPDAFEREAMQELGVPRQ
                     IPMRHRSTQFGHIFSGEGYSAGYYGYMWADVLTADAAEAFAEAPGGFYDEAVAERLVQ
                     YLFAPRNSVDPADAYRQFRGRDARIDALMRDRGFPVEKAGET"
     gene            complement(3247..3780)
                     /locus_tag="VFY27_03470"
     CDS             complement(3247..3780)
                     /locus_tag="VFY27_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764599.1"
                     /translation="MSDVFYLRPIVPPATPEAVVRLASEAGGCFDLHRVAWTLSFLAA
                     DGGRMLCWYRAPDAESARMALKELGSDMNAVWPGTVVGDLRADDLAISQVAALAEISF
                     AQPLPGGHQEVLQRFGHESLRDVPVAAGFLSSDRRHLVALLQATNACTAGAALAAASL
                     PVDSFWPCTVIAPPRPV"
     gene            3818..3979
                     /locus_tag="VFY27_03475"
     CDS             3818..3979
                     /locus_tag="VFY27_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764600.1"
                     /translation="MSSRRHREGDRGPKLAESANQQRAASKMLPAGYQDYHRILETHL
                     GRIVMDNTM"
     gene            4012..>4707
                     /locus_tag="VFY27_03480"
     CDS             4012..>4707
                     /locus_tag="VFY27_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764601.1"
                     /translation="MAARKKSSRASSSKSSGNGAARTIIYVHGIGNKPQPHVLKCQWD
                     NALFGFDLGERSRLAYWVNRAWYPEPSSGTCATGDLVTPQELDGPPSLGARALTAEEP
                     MEDFVAELNGDKAARRTLLAIARKLDREPTVTTEKSLHAHEVDAKVSPFPKPVRRWLT
                     RRLTGALLRDVNDLFYVEERREIMRNSLKERILAGGGPFVIIGHSQGSMIAYDVLSEM
                     SGNRDAPDVALFVT"
BASE COUNT          999 a         1479 c         1484 g          745 t
ORIGIN      
        1 ccttcatgat ccaggggctg ctcgcggctc gccagtactt cgacgaagac accgcgatcg
       61 agcgcgaaat ccgcagcacc atcacgcggc tatggcggga ggtcgagtgg aactggtacc
      121 ggaaaactcc cgacggcgag gtgctgtact ggcactggtc gccggagcac gcgttccaca
      181 tcaatcaccc cctcatcggc tggaacgaga cgatgatcgt ctacctgctg gccgtcgcgt
      241 cgccgacgca cgccgtgccg gcggagcttt accacacggg ctgggcggga acgtcggacc
      301 tgcacacctg gtaccgccag ggctggggac gcaccaagga cggcaaccga tacgtcaacg
      361 gcaacacgta ctacggcaaa aagctcgacg tcggcgtcgg tgccggggga gacctgttct
      421 tcgcccactt ttcctacatg ggtttcgacc cgcgcggcat tcgcgaccgt tccaccaact
      481 acttcgacaa taaccgcgcc atagcgctga tcaaccatgc ctactgcgtc gagaatcccc
      541 tgggcttcga aggatatggg ccggacacct ggggcctctc ggccggcgtc tacaccggcg
      601 gcggacgacc acagcctcga gacgacaacg gcacaatcaa cgtcatggca tcccttgcct
      661 ccatgccgta cacgcccgag aaatcgatgg cagcgctgaa gcactactac cgggacctcg
      721 gcggcaaagt atggggcgcg tatggttttc acgacggctt caacgcaaca gagaactggt
      781 tcgacgaggt atacatggcg ctgaaccagg cgccgatcac cgtgatgatc gaaaaccacc
      841 gcagcggcct ggtgtggaaa cagttcatgg caaacccgga gatcaagcct gcgctggacg
      901 caatcggctt cgtcgatgac gaggcgccct gacggctcgc acatggcacg ggtgacgatc
      961 tgccggcata gcacagcgga tgcgatagca tggaagcatg cgaagagggg ccggaccggg
     1021 cccgccacgt aaagggatcc aaccggatga acggagtaat acggatggtt ctgatcgcgt
     1081 cttgcggcgc ggtcctcgga tgtaccgaca acccgaacgg cgacacccgc caaagcggga
     1141 acgcaccacc ggtggaggcc cctgtggaag aaggcgacat gcagaacccg ttgctggccg
     1201 agtggacagg tccctacggc ggcttgccgg ccttcgacaa gatgcacctc gagcatttga
     1261 agccggcgct ggaacaggct atggaacaga ggcttaggga gatagactcg atcgcgagca
     1321 acggcgagcc gccgaccttc gagaacacca tccttgccct gcaacgcgaa ggtaaggcgc
     1381 tggaccgcgt ccggacctac tacggtttgt ggcgctccaa cctctcgacg ccggagtttc
     1441 gcggcatcca ggaggagatg gaaccaaggc ttgctgcaca cgaatccgcc attacgcaaa
     1501 acgatgcact gttcgaccgc gtgcgcgccg tctacgagag ccacgaactg aagatgcgga
     1561 caccggacga acaacgcctg gtccggctca tctacgacga cttcgcgcgc aacggcgcga
     1621 cactcgaggg cgaggcaaaa gcgcgctatg ccgaaatcga ccagcggctt tcggaactcc
     1681 acacccggtt tgcgaacaac gttctcgccg acgaggaaaa gtacgtgctg tgcatccgcg
     1741 ggaaccagct cggcggactt ccggcttcct tcgtgcaggc cgccagggca gccggcgagg
     1801 agcgttgtgc cgccggggag tatgccatca ccaatacccg ttcctcgatg gatcctttcc
     1861 ttacctggtc cgaccagcgc gatttgcgcg agcaggtgtg gcgaacctat tacaaccgcg
     1921 gcgacaacga cgacgagaac gacaacaacg cgatcatcgc cgagatactg aagttgcgcg
     1981 atgagcgggt ggggctgctg gggtatgaca actacgcgca gtggcggctc gaagaccgga
     2041 tggcaaagac ccccgagaat gcgctcgcac ttctaagggc ggtattgccg gcggcacttg
     2101 cgcgcgtgga cgaagagatt gcggacatgc aggcaatcgc agatgaacgc ggcgacggga
     2161 tcgaaataga ggcctgggac taccgctact acgcagagct ggtgcggcgc gaccggtatg
     2221 cgctcgactc ggaccaggtg aagcaatacc tgcagctgga caagctgcgc gacgcaatgt
     2281 tcttcgtcgc gggggaactg ttcgatttcg agttcaagcc ggtgccggag ggctcagtgc
     2341 cggtgtttca tcctgacgtg aacgtatggg aagtaaccga caggaccagc ggcgagcacg
     2401 tcgggctgtg gtacctggac ccgttcgcga ggcccggcaa gaggtcaggc gcatgggcga
     2461 gcacctatcg cggtcactcg acgttcgacg gacgagaaac ggtactctct tccaacaatt
     2521 ccaacttcat caagggagcg ccgggcgagc cggtactgat ttcctgggat gacgcgacga
     2581 cctacttcca cgagttcggt cacgcgctgc acgccttgtc atcgaacgtc cgttacccga
     2641 cgctgaacag cggcgtacgg gactacaccg aattccattc acagctgctc gaacgatggt
     2701 tgcctaccga gccggtcgtc gggaactacc tcgtgcactt cgagacgggg gagccgatac
     2761 ccggcgagct ggtggcaaag atcgagaacg cggcgacgtt caaccagggc ttcgagacga
     2821 ccgaatatct tgcctccgca ctggtcgaca tgatgtatca cacgaccgac cccgccgaaa
     2881 tcgatcccga tgccttcgaa cgcgaggcga tgcaggaact cggggtcccg cggcagatcc
     2941 cgatgcgcca ccgttccacc cagttcgggc acatcttttc cggcgaaggg tattcagccg
     3001 gctactacgg ctacatgtgg gccgacgtgc tgacggcgga tgcagccgaa gcgtttgcgg
     3061 aagcgcccgg aggcttctat gacgaggccg tcgccgagcg gctggtgcaa tatctgttcg
     3121 cgccgcgaaa ttccgtcgac ccggcggacg cgtatcgcca gttccgcgga cgcgatgcgc
     3181 gcatagatgc gctgatgcgc gaccgcggct tcccggtgga gaaagccggc gaaacctgat
     3241 acgaaactac accgggcgcg gcggcgcaat gacggtacaa ggccagaaag agtcgaccgg
     3301 cagcgaagcg gccgcgagag cggcacccgc tgtgcaggca tttgttgcct gtaacagcgc
     3361 gaccaggtgc ctgcgatcgc tggacaggaa cccggccgct accgggacat cgcggagcga
     3421 ctcatggccg aagcgctgca acacctcctg atgtcctccc gggaggggct gagcgaaaga
     3481 tatctcggcc agcgcagcga cctgcgatat cgccaagtca tccgcgcgaa ggtcgccaac
     3541 cactgttcca ggccacacgg cattcatgtc cgagcccagt tccttcaggg ccattctcgc
     3601 cgactccgca tccggcgcgc gataccagca caacatgcgg ccgccgtcgg ccgccaggaa
     3661 gctcaatgtc caggcgaccc tgtgcaggtc aaaacagccg cccgcctcgc tcgccaggcg
     3721 aactacggct tccggcgtgg caggcggcac aatcgggcgc aggtaaaaaa cgtcgctcac
     3781 gcgaagaata tagcgcaact ggggcagagc ctgcctcatg tccagcaggc gccaccgcga
     3841 aggcgaccgt gggccaaagc tggcagaatc tgcaaatcag cagagggctg catcgaaaat
     3901 gcttccggcc ggctaccagg actatcacag gattctcgag actcacctgg ggcgaatcgt
     3961 gatggacaac acgatgtagt gccggtatag acaaaactga aagggggatc catggccgcc
     4021 aggaagaagt cctcgcgcgc gtcgtcatcg aagagttccg gaaacggcgc cgcccgcacg
     4081 atcatctatg tccacgggat cggtaacaaa ccgcagccgc acgtcctgaa gtgccagtgg
     4141 gataacgccc tgttcggctt cgacctcggc gaacgcagcc ggcttgccta ctgggtcaat
     4201 cgcgcctggt acccggagcc ttcctcgggc acctgtgcta cgggcgatct cgtgacgccg
     4261 caggagcttg acgggccgcc gtccctcggc gcccgcgcat tgacggccga ggagcctatg
     4321 gaggattttg tcgcggagct gaacggtgac aaggccgccc gccggacgct gctggcgata
     4381 gccaggaagc tcgaccgcga gccgaccgtg acgacggaaa agtcgctgca cgcgcacgag
     4441 gtcgacgcaa aggtctcgcc ctttcccaag ccagtcaggc gatggctgac ccggcgcctg
     4501 acgggcgcgc tgctgcgcga cgtcaatgac ctgttctacg tggaggagcg gcgcgagatc
     4561 atgcgcaact cactgaagga gcgcatcctg gccggcggcg gtcccttcgt catcatcggt
     4621 cacagccagg gctcgatgat cgcctatgac gtcctgagcg agatgagcgg gaaccgcgac
     4681 gcgccggacg tcgctctgtt cgtcacc
//
LOCUS       DASUOM010000172         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_493699,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000172 DASUOM010000000
VERSION     DASUOM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493699"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VFY27_03485"
     CDS             <1..1371
                     /locus_tag="VFY27_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764602.1"
                     /translation="GGQNILEEVTAFQLDLERDLMVGERATTIKFGAKRVSRDKTSDQ
                     DLEVFDGFADDLLLTGLTDPGKDDFYSSEREYEFGPRMNWPSLEAFFNGNAGGFEQNA
                     ADTIAESFGVDFDVSEDVTAGYVMGTMDFGRATVTGGVRVERTETDFSAFDLVFLDGD
                     AETPVPVTGDKSYTHWLPSLHLRMELRDDLLLRAAWTNTIGRPSYEVNVPFRIFEIEE
                     EEDNPDVFEGEAEQGNPDLEPLESMNLDVALEWYFESGGILAAGVFYKDIDKPIFTRF
                     TELEDEVFEGREFSELVVVTTDNADSGEIIGLELNYQQQFLGLPSPFNGFGVAFNYTF
                     TDSEATVFDRDDKVPFFLQSDHIGNAALFWERSGLEARLAYTYYSTYLDTLGDEESQD
                     LYFDKRGQLDFKTSYELTDYVNVFFEWHNITDEPLRFFSGKESGRLAENEIYGWNVVG
                     GVQVKF"
     gene            1501..2325
                     /locus_tag="VFY27_03490"
     CDS             1501..2325
                     /locus_tag="VFY27_03490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5764603.1"
                     /translation="MSAKPAAKVSKKTMAEKADIHDLYEKSVQDVEMETEFLQKTFTE
                     LSGRKAHLFREDFCGTASACCQWVKQGKDYRAIGVDIDPEVLDWGRRNRLARLSAAEQ
                     ARVRMIESDVLSVEAPPVDLIAALNFSYFIFDTRERLRTYFRRCFEALKEDGVLFLDM
                     YGGPEAQEETREKTKHKKFTYIWHQAEFHPVTHFARCHIHFKFPDGSKIKKAFTYEWR
                     LWTAPELRELLLEAGFAKATVYWEGEDEDGEGNGIFTPDEKGVADLAWIAYVVGEK"
     gene            2577..3092
                     /locus_tag="VFY27_03495"
                     /pseudo
     CDS             2577..3092
                     /locus_tag="VFY27_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            3255..3512
                     /locus_tag="VFY27_03500"
     CDS             3255..3512
                     /locus_tag="VFY27_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5764604.1"
                     /translation="MGRADDNDLVVKGNLISRIHARVEKRRGKFLLVDQSTNGTFLQN
                     ARGEETFVRRDSTELFGEGIIGLGRVAKPGTPLAIHFICEE"
     gene            complement(3676..>5379)
                     /locus_tag="VFY27_03505"
     CDS             complement(3676..>5379)
                     /locus_tag="VFY27_03505"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX5764605.1"
                     /translation="AKLTQPEDIHWCNGSESEFKRLINQMNRTGELLELDQHTYPHCY
                     LHRSHPSDVARVEHLTYVCTTDKADAGPNNNWMSPEEAHSLVDDLFDGAMQGRTMYVI
                     PYCMGPIDSPYARLGVEITDSAYVVVNMKLMTRIGSAALARIEKEGRFVKGLHSIGDL
                     DPDRRFIMHFPEELSIKSIGSGYGGNALLGKKCHALRIASWQARQEGWLAEHMLIVGI
                     ESPEGETHYIACAFPSACGKTNLAMLIPPESHAGWKIWTLGDDIAWMHLDHEGRLRAI
                     NPEAGYFGVVPGTNAKTNRNAFDMIRSDTIFTNVALTAENQPWWEGKKTGKPVVDWQG
                     RKYEAGNGPAAHPNSRFTVSAKKNPVYSELADAPEGVPISALVFGGRRKEVAPLVFEA
                     ADWRHGVLVGAGVASETTAAASGAVGVVRRDPMAMKPFCGYNFADYWAHWLSFAEKSD
                     RLPRIFHVNWFRQDSSGKFLWPGFGENLRVLRWIIDRCEGRVGARETPIGFVPRPGDI
                     DAEGLEIDHDILEVLLSVDVNQWKTEMESIGEYLAGFGDRLPPELKQEYQRVSSALRN
                     A"
BASE COUNT         1116 a         1596 c         1628 g         1039 t
ORIGIN      
        1 ggtggccaga acatcctcga ggaggtaacc gcatttcagc tcgacctgga acgcgatctg
       61 atggtcggcg agcgtgcgac gaccatcaag ttcggcgcca aacgcgtcag ccgcgacaag
      121 acgtcggacc aggacctcga agttttcgac gggttcgccg atgacctcct gctcaccggc
      181 ttgaccgatc cggggaagga tgatttctat tccagcgagc gcgagtacga gtttggcccg
      241 cggatgaact ggccctcgct cgaggctttc ttcaatggca acgccggcgg cttcgagcag
      301 aacgccgcgg acacgatcgc cgaatcgttc ggcgtggact tcgatgtgtc cgaggatgtc
      361 accgcgggtt acgtcatggg aaccatggat tttggccgtg ccaccgtcac cggtggagtt
      421 cgcgtcgaac gcacggagac ggacttctcg gcattcgacc tggtgttcct ggacggcgat
      481 gcggagactc cggtgcctgt cactggcgac aagagctata cccattggct gccgagcttg
      541 cacctgcgca tggaactgcg cgacgacctg ctcttgcgcg cggcctggac gaacaccatc
      601 gggcggccgt cctacgaagt caacgtgccc ttccgcatct tcgagatcga ggaagaggag
      661 gacaatcccg atgttttcga gggtgaggca gagcagggaa acccggacct cgagccgctg
      721 gaatccatga acctggacgt tgcgctggag tggtacttcg agtcgggcgg catactggcg
      781 gccggtgtct tctacaagga catcgacaag cccatcttta cacgattcac ggaactggag
      841 gacgaggtgt tcgaaggacg tgagttcagc gaactcgtag tggtgacgac cgataacgcc
      901 gattccgggg aaattatcgg gctggaactc aactaccagc agcagttcct cggattgccg
      961 tcgccgttca acgggttcgg cgtagcgttc aactatacgt tcacggatag cgaggcgacc
     1021 gtgttcgatc gcgacgacaa ggttccgttc ttcctgcagt cagaccacat tggcaacgct
     1081 gcactcttct gggagcgaag tggcctcgag gcacggcttg catacacgta ctacagcacc
     1141 tacctcgata cgctgggcga tgaggaatcg caggatctct acttcgacaa gcgcggccaa
     1201 ctcgatttca agacgagcta cgaactcacg gattacgtga acgtgttttt cgaatggcac
     1261 aacatcaccg acgagccgct gcgctttttc agcggcaagg aaagcggccg gctggccgag
     1321 aatgaaatct acgggtggaa tgtcgtgggg ggcgtgcaag ttaagttctg acgatcaggg
     1381 agctctgcac gccagacgct cgcaggcttc ggcctgcgag cgttttttta ttcatggcag
     1441 gccctcgcgg ctgtcgatta caattcatcg ctcactcgca aagcggagaa gttaccgacc
     1501 atgagcgcaa agcccgccgc aaaagtttcc aaaaagacga tggccgagaa ggccgatatc
     1561 cacgatcttt atgagaagtc ggtgcaggac gtggagatgg aaacggaatt cctgcaaaag
     1621 acgtttacgg aactgagcgg acgaaaggcg cacctgttcc gggaagactt ctgcggcacg
     1681 gcgagtgcct gttgccaatg ggtaaaacag ggaaaggact acagggcgat cggcgtcgat
     1741 atagacccgg aagtgctgga ttggggccgc aggaacaggc ttgcgcgcct ttccgcggcc
     1801 gagcaggcca gggtccggat gatcgagtcg gacgttttgt cggtggaggc cccgccggtg
     1861 gacctcatcg ctgccctcaa tttcagctac ttcatcttcg acacccggga gcggctacgc
     1921 acctacttcc ggcgatgctt cgaagcgttg aaagaagacg gtgtcctgtt tctcgacatg
     1981 tacggcggtc cggaagcgca ggaagagacg cgcgagaaga ccaagcacaa gaagttcacc
     2041 tatatctggc accaggcgga gtttcatccg gtaactcatt tcgcccgctg ccacatccac
     2101 ttcaagttcc ccgatggctc caagatcaag aaagcgttca cctacgagtg gcgattgtgg
     2161 acggccccgg agcttcgcga gctgctgctg gaggcgggct tcgcaaaggc gaccgtgtac
     2221 tgggaaggcg aagacgaaga cggggagggc aacggtatct tcacgcccga cgagaagggc
     2281 gtggccgacc tcgcatggat tgcctacgtc gtgggcgaga aatagcggct ccgcgaaaac
     2341 atgatctccg tccgggcgag agcgaaaatc acgaatgtaa cctgcccctg tttcctctct
     2401 ctgcaatagc gtaatattct cctcgaaaac ggccgggatt ttaggtgctt gctgcagcaa
     2461 cgcaataagc gactgagatt cgcgcattgg acataatatt cgccgatcct gcggctctcc
     2521 gcttataatc gccggtccgg cagcattggc ccgggccacg ctgtcgccga gcttcgcaca
     2581 agagactcgc acaagaagag aaaagcccaa gaatggcgaa agacctggaa gtcgcaatac
     2641 tgttcgctga cgttgtcggc agcacacagc tgtacgacga gtatggtgac accaaggcga
     2701 gcgagaccgt cgcacactgc ctcgacgtca tgaaagatgc gacgcaccag ttcaacggca
     2761 ccgtcatcaa gaccatcggc gacgaggtca tggccacatt cccgacggta gacgacgcga
     2821 tggcagcggc cagccagatg cagggccgcc tcagcagcga aggcgatggc gacaaggatc
     2881 acataccggt gtcgatccgc atagggtgtc actacggtcc tgtggtccag gaacacaatg
     2941 acatcttcgg cgccgccgtg cataccgcca atcgaatgac gtcgcaggcg aaggcccggc
     3001 agatcgtgat ttccggtacg accgtggacc gcatgagcga cgagtggcag gcccagacgc
     3061 gacagatcga cgttgcgact gtgcgcggcc ggctggacga ggtagcgctg ttcgaactca
     3121 tctggcagcc cgaagaggtc accagcatgc tgcccacgat cgagtgggag agcaaagcga
     3181 ggaagaccag ccgtatcacg ttgagcttcc gtgacaccac cgtcgtggtc gacgaccgta
     3241 agaagaacat aaacatggga cgggccgacg acaacgacct cgtcgtcaaa ggcaacctga
     3301 tttcccgcat ccatgcccgg gtagagaaac gtcgcggcaa attcctgctg gtggaccaga
     3361 gcaccaacgg caccttcctg cagaacgcgc gcggcgaaga aacgttcgtg cgccgcgaca
     3421 gcaccgagct gttcggcgag ggcatcatcg ggctgggccg cgtagcgaaa cccggcaccc
     3481 cgctcgcgat tcatttcatc tgcgaagaat aagcgcgcac ggatcggacg atggtcgccc
     3541 gtgggaaggg ctctcgcagc cttccaccac acaatggata gcaagctgta ggagccctcc
     3601 atgcgggcga gttcgttgac gcctgatcac actcgcccgc atggcgggct cctacggttt
     3661 catcaatccg cccagtcagg cgtttcgcag ggcgcttgag actctctggt attcctgctt
     3721 cagttcgggc gggagccgat ccccgaaacc ggcaaggtac tcgcctatcg actccatttc
     3781 cgttttccac tggttcacgt cgaccgagag cagaacttcg aggatgtcat gatcgatttc
     3841 cagtccctcg gcgtcgatgt cgcctggcct gggcacgaag ccgatgggcg tttcgcgcgc
     3901 tccgacgcgc ccttcgcatc ggtcgatgat ccagcgaagc acgcggaggt tctcgccgaa
     3961 gcccggccac aggaacttgc ctgaagaatc ctggcggaac cagttgacgt gaaagatcct
     4021 cgggagcctg tcggattttt ccgcgaacga cagccagtgg gcccagtagt ctgcaaagtt
     4081 gtagccgcaa aacggcttca tcgccatcgg gtcccggcgc accacgccca cagcaccgct
     4141 tgcggcggcc gtcgtctcgg acgcgacgcc cgcgccgacc aggacaccgt gccgccagtc
     4201 tgcggcctca aaaacgagcg gtgccacttc cttccggcgt ccgccgaaga ccagcgctga
     4261 aatcggcaca ccttccggag cgtcggcgag ttcggagtaa accgggttct tcttcgccga
     4321 caccgtaaac ctggagttcg ggtgcgcagc gggtccgttc cccgcttcgt acttgcgccc
     4381 ctgccagtcc accacgggct tgcccgtttt cttgccttcc caccacggct ggttctcggc
     4441 ggtcaacgcg acgttagtga aaatcgtgtc gctcctgatc atgtcgaacg cattcctgtt
     4501 cgtcttcgcg ttcgtgccgg gcacaacccc gaagtacccg gcttcgggat tgatggcgcg
     4561 caggcggcct tcgtgatcga ggtgcatcca ggcgatgtcg tcgcccagcg tccagatctt
     4621 ccagcccgcg tgcgactccg gcggaatcag cattgccagg ttcgttttgc cgcacgcaga
     4681 gggaaacgcg caggcgatgt agtgcgtttc gccctccggg ctttcgatgc cgacgatcag
     4741 catgtgctcg gcgagccatc cttcctgccg cgcctgccag ctggcaatgc ggagcgcgtg
     4801 gcatttcttt ccaagcaagg cgtttccgcc gtagcccgag ccgatgctct tgatcgacag
     4861 ttcctccggg aaatgcatga tgaaacgcct gtccggatcg aggtcgccta tggagtgcag
     4921 gcccttgacg aatcgccctt ccttttcaat acgcgcgagt gcagcagaac caatgcgtgt
     4981 catcagtttc atgttgacga cgacgtaagc actgtcggtg atttccactc ccagccgtgc
     5041 gtatggcgag tctatgggtc ccatgcagta agggatgacg tacatcgtcc tgccctgcat
     5101 tgcgccatcg aacaggtcat cgacaaggct gtgagcctct tcgggcgaca tccagttgtt
     5161 gttcgggccc gcatcggcct tgtccgtcgt gcagacgtac gtcaggtgtt cgaccctggc
     5221 gacatctgaa gggtgcgaac gatgcaggta gcagtgcggg taggtgtgct ggtcgagctc
     5281 gagcagttct cccgtccggt tcatctggtt gatgagccgc ttgaattccg attcgctgcc
     5341 gttgcaccag tggatgtctt ccggttgcgt caacttcgc
//
LOCUS       DASUOM010000173         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1000631,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000173 DASUOM010000000
VERSION     DASUOM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000631"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VFY27_03510"
     CDS             complement(<1..1904)
                     /locus_tag="VFY27_03510"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5764606.1"
                     /translation="MDYAATILAKLPGTSVRRDHGLRRLIETLESYLPREHIEQVIQA
                     YEFGASAHQGQKRLSGEAFISHPVAVAQTLADMRMDARAITAAILHDVIEDTATPVAE
                     LEEQFGSEVAALVDGVSKLDQMHFNSRAEAQAETFRKMMLAMIEDIRVILVKLADRLH
                     NMRTFDAMPREKQVRIAKETLEIYAPIANRLGINHMKVDLEDLGFRYLYPFRYRVLEK
                     AVRRSKGSQRQIVKKISERIRTGMKDAGIEGEVIGREKHLYSIYKKMSEKKRLLSDVV
                     DVYGFRIIVGDVNTCYQTLGLVHGLYKPMPGRFKDYIAIPRINGYQSLHTTLFGPKGL
                     PLEVQIRTSDMDRVAESGVAAHWQYKAVDKTTATPQRRAREWLAHLVEIQESGSSEEF
                     LESVKVDLFPDKIYVFTPQGEIKPLPKGATTVDFAYAVHTDVGNRCVAAKIDRNLVPL
                     RTQLQNSQTVEIITARGARPNPNWLTFVATAKARSAIRHHMKKMRSSESVDLGKRLLD
                     RSLKDLGSSLRKVGRVRLREALKELGLSEPAELYEQLGLGERLAPLTARLLVGAGSEN
                     GAPRTASLTIAGTEGVVVSYAKCCHPVPGDEVMGYLSSGRGVVIHRNVCGNLSNFRKQ
                     SEKWIAVTWE"
     gene            complement(2012..2293)
                     /gene="rpoZ"
                     /locus_tag="VFY27_03515"
     CDS             complement(2012..2293)
                     /gene="rpoZ"
                     /locus_tag="VFY27_03515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5764607.1"
                     /translation="MARITVEDCLDNVDNIFEMVLVAAKRARRIAHGADTLVDPENDK
                     PTVIALREIAAGHITPSILDEVEQPATDEFLQPDTADDIIPMRGISIGD"
     gene            complement(2451..>2853)
                     /locus_tag="VFY27_03520"
     CDS             complement(2451..>2853)
                     /locus_tag="VFY27_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5764608.1"
                     /translation="VFDNRYGTGRAQVEANLARGSDVILEIDWQGARQVRQSMPECQS
                     VFILPPSLRELERRLRDRRTDSEPVILRRLRDAIGDISHWNEFDFVIINDDLRKSVLE
                     LEAIISGRGDCRENRTSHPDMRQRIREILGA"
BASE COUNT          501 a          891 c          863 g          598 t
ORIGIN      
        1 ttctcccagg tcaccgcgat ccacttctcc gattgttttc ggaagttgct caggttgcca
       61 cagacatttc tgtgaataac cacgcctcga ccggagctca ggtaacccat gacctcgtcg
      121 ccagggacgg gatggcagca cttggcgtag ctgacgacca cgccctccgt gccggcaatg
      181 gtgaggctgg cggtgcgtgg cgcgccattc tcgctgccgg ccccgaccag cagcctcgcc
      241 gtcagcgggg ccaggcgctc gcccagtccc agttgctcgt acaactcggc aggctccgaa
      301 agacccaact ccttcagcgc ctcgcgcagg cgcacccttc cgaccttcct gagtgaagag
      361 ccgagatcct tgagcgaccg gtccaggagg cgcttgccca ggtccaccga ctcgctgctg
      421 cgcatcttct tcatgtggtg ccggatggcc gaacgagctt tggccgttgc tacgaaggtc
      481 agccagttgg ggttcggccg cgccccgcga gcggtaatga tttccaccgt ctggctgttt
      541 tgcagctgcg tgcgaagcgg cacgaggttg cggtcgatct ttgccgccac gcaacggttg
      601 cctacatcgg tatgcacggc gtaggcaaag tccaccgtcg tcgctccctt cggcagcggc
      661 ttgatctcgc cctgcggggt aaaaacgtag atcttgtccg ggaacaggtc gaccttgacg
      721 ctttccagga actcttccga gctgccggat tcctggattt caaccaggtg tgcgagccat
      781 tcgcgcgcgc ggcgttgcgg ggtcgcggtc gtcttgtcga cggccttgta ctgccagtga
      841 gctgcgacac ccgactccgc gacacgatcc atgtcactgg tgcgaatctg cacctccagc
      901 ggcaggccct tgggaccgaa cagcgtggtg tgcagcgact ggtacccgtt gatgcgcggg
      961 atggcgatgt agtccttgaa gcgacccggc ataggtttgt aaaggccgtg cacgagaccg
     1021 agcgtctggt agcaggtgtt cacatcgccg acgatgatgc gaaaaccgta cacgtcgacc
     1081 acgtccgaga gcaggcgttt cttctcggac atcttcttgt aaatgctgta gaggtgcttc
     1141 tcgcgcccta taacctcgcc ctcgatgcca gcatctttca tcccggtgcg gatgcgctcg
     1201 gagattttct tgacgatctg gcgctggctt cccttgctcc tgcgcaccgc cttttcgaga
     1261 acgcggtagc ggaacggata gagatagcgg aagcccaggt cctccaggtc gaccttcatg
     1321 tgattgatgc cgagccggtt cgcgatgggc gcgtagatct cgagcgtctc cttcgcgatc
     1381 cgcacctgct tctccctcgg cattgcgtcg aaggtccgca tgttgtgcag ccggtcggcg
     1441 agcttgacca ggatcacgcg gatgtcctcg atcatcgcca gcatcatttt gcggaaggtc
     1501 tccgcctgcg cctcggcgcg gctgttgaag tgcatctggt cgagcttgct gacgccgtcg
     1561 accagtgccg ccacttcgct gccaaactgt tcttccagtt cggcaaccgg ggttgcagtg
     1621 tcctctatta cgtcatgcag gatggccgcg gtgatggccc tggcgtccat tcgcatgtcc
     1681 gcaagagtct gggcaaccgc gacaggatgg gatatgaacg cttcgccgct caggcgcttc
     1741 tgcccctggt gggcgctcgc gccgaactcg tatgcctgga tgacctgctc gatgtgctcg
     1801 cgcggcaggt aggactcgag agtttcaatc agccggcgca gaccgtggtc gcgtcgtacg
     1861 gacgtacccg gaagtttggc gagaattgtc gcagcgtagt ccatgaccga tcatacccgg
     1921 ttgccgggcg agccgccgcg accggaaaac gcgaagctaa atgttcctcg aaaacccctt
     1981 gagatcgtcg ctccctgccg ggagcgcgcg atcagtcacc aatcgatatg ccacgcatcg
     2041 gaatgatgtc gtctgcggta tccggttgca gaaactcatc cgttgccggc tgctctacct
     2101 cgtccaggat cgacggcgtg atatgacctg ccgcgatctc gcgcagcgca atgaccgtgg
     2161 gcttgtcgtt ttccgggtcg accagagtat cggcgccatg cgcaatgcgg cgagcgcgtt
     2221 tcgcggccac cagcaccatc tcaaaaatgt tatcgacgtt gtcgaggcag tcttcgaccg
     2281 taattcgggc cattaaaaag gtactccaaa ggtcattgcg tcaaggtcat tgcatccgcc
     2341 cgtcctcaaa agaggcgggg tcaggagcga atagtcgtta ctggaacaga cccgccagtg
     2401 tacttcaagt tcaacggccg ggggaaccca aaggagaagc cgcacggggc tcaggccccc
     2461 aggatctccc tgatgcgctg ccgcatgtcc ggatggctgg tcctgttctc ccggcaatcg
     2521 ccgcggccgg aaataatggc ctccagctcc agcaccgact ttctcaggtc atcgttgatg
     2581 atgacgaagt cgaactcgtt ccagtgagag atgtcgccta ttgcatccct gaggcgccgc
     2641 aggatgacgg gttcgctgtc ggtacggcgg tcccgcaggc gccgttccag ttccctgagc
     2701 gatggcggca ggatgaagac ggactggcac tccggcatcg attgccgtac ctgccgggct
     2761 ccctgccagt cgatttcaag gatcacgtcg ctacccctgg caaggttcgc ctcgacctgg
     2821 gcgcgtccgg tgccgtagcg gttgtcgaat acc
//
LOCUS       DASUOM010000174         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_146885,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000174 DASUOM010000000
VERSION     DASUOM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146885"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFY27_03525"
     CDS             complement(<1..1268)
                     /locus_tag="VFY27_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX5764609.1"
                     /translation="MIFSTNPRLLVLVPILLLVSASVPAGTIMSAGKPAQLNIRLAGT
                     ASLRVTLKPLDYAREFPDSPAIARGRWPEPNIELEELDGILNRRIGPLYVTVSDDPLT
                     IDVSAADGRSVQNLVFASDGTVSFRLDDRPVLGLGGGGPTMGENWREEPLEFDRRGRL
                     HEMTPRWQQKAYGSRNPVPLLVGTSGWALFMNSPWVQVDLRDERRGLFIPWTPPEAGS
                     AAPADLQGRPPPESAVEGLVDFFLFDAGEPGTFMTDVARIAGRPAMPPKWALGYMQSH
                     RLLEDEQQMIDIVNTFRRKNLPIDAVIYQGTGYTPKGWNTEQPSFTFNPELFTREPSE
                     VIRDLHERNVKVIVHVVPPSTEELPHLHGNVPPRETETVDQSHIQAHWQDHVPLLESG
                     IDGFWPDEGDRFDLPSRLNRHKMYYQGPLSV"
     gene            complement(1348..2184)
                     /locus_tag="VFY27_03530"
     CDS             complement(1348..2184)
                     /locus_tag="VFY27_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX5764610.1"
                     /translation="MDPGTLYRLAAFSDRSDGGNPAGVWVGKALPAADVMQRIAASVG
                     YSETAFVAPGAGQRRTVRYFSPEAEVSFCGHATIATGVALGSVEGDGTYTLDTRVGQV
                     PVTVRSRNGIREASLTSVEPRYEPAADDLVTNALQALNWRRDQLDDSIPPARAYAGAW
                     HLVLAAAESGRLSHLDYHFDQLKALMLRDGLTTLQLVWRESELVFHSRNPFPVGGVVE
                     DPATGAAAAALGGYLRALRSVSPPASVLVRQGEVMGRPSRLSVDIPVSGGIVVTGTAV
                     PI"
     gene            complement(2218..2829)
                     /locus_tag="VFY27_03535"
     CDS             complement(2218..2829)
                     /locus_tag="VFY27_03535"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX5764611.1"
                     /translation="MNEQARQLRALDRVGGHPALDFVNTVNSWTGGVPGTEYLRTYTD
                     ILDWHQAAGMIGAGSRRALSRNPLKRQEAAVKKVTALRGALRALFHAVAARKPLPQRS
                     LDRLNEVIRETARWRRLVAHGGEISCGWDFEGAPPEAVLGPVAWQAAELLEHGGLDRV
                     KECPTDEGCGWLFLDLSKNRSRTWCSMKTCGNSAKVKRFRARQ"
     gene            3090..3473
                     /locus_tag="VFY27_03540"
     CDS             3090..3473
                     /locus_tag="VFY27_03540"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5764612.1"
                     /translation="MHHSRLGTLMIDCLDHEFDASFTFWSEALNLAPHRRPSRKQRYA
                     TVGKIEGPLFVRLQRVDNNPGYHLDIETDDLLAEVARLKGHGARNQRRVKRWWVAEDP
                     SGNAFCVIRPESDDFPRNANPWEGK"
     gene            3475..>3735
                     /locus_tag="VFY27_03545"
     CDS             3475..>3735
                     /locus_tag="VFY27_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764613.1"
                     /translation="MIMREWRGRLPHDKVDEYLQYLRVTGLNEYANTPGHHGTWVLLD
                     RHLDGKPGIAEYTLLTLWESRDAIRAFAGDDIEKAKYYPEDEH"
BASE COUNT          694 a         1199 c         1133 g          709 t
ORIGIN      
        1 accgaaagcg gcccctggta gtacatcttg tggcggttaa gcctggacgg caggtcgaag
       61 cggtcaccct catccggcca gaagccatcg atgcccgatt cgagcagcgg aacatggtcc
      121 tgccagtgcg cctggatgtg actctggtcg acggtttcgg tttcccgcgg cggcacgtta
      181 ccgtgcaggt gcggcaattc ttcagtggac gggggcacta cgtgcacgat tactttcacg
      241 tttcgctcgt gcaggtcgcg gatgacttcc gacggttccc gtgtgaacag ctccgggttg
      301 aacgtaaaag acggctgctc cgtgttccag cccttgggcg tgtaaccggt gccctggtag
      361 atgacagcat cgatcggcag gttcttcctg cgaaacgtgt tgacgatgtc gatcatctgc
      421 tgttcgtctt ccagcaggcg gtgtgactgc atgtagccga gcgcccattt cggcggcatc
      481 gcgggcctgc cggcaatgcg ggcgacgtcg gtcatgaagg ttcccggctc gccggcatcg
      541 aaaaggaaaa aatccaccag tccctctact gcgctttccg gaggagggcg cccctgcaaa
      601 tcggccggtg ccgcggagcc ggcctccggc ggcgtccacg gaatgaacag gccgcgccgc
      661 tcgtcgcgca ggtccacctg cacccacgga gaattcatga agagcgccca gccggacgta
      721 ccgacgagca acggtaccgg gttccgtgag ccgtaggcct tctgttgcca gcgcggcgtc
      781 atctcgtgca gtcggccgcg acggtcgaac tcgagcggct cttcgcgcca attctctccc
      841 atggtcgggc cgccccctcc gagccccagt accgggcgat catcgaggcg gaatgaaacc
      901 gtaccgtcac tggcgaagac caggttttgc accgaccggc cgtcggctgc gctgacatcg
      961 atagtcagcg ggtcatcact gaccgtcacg tagagaggcc cgatgcggcg attcaggatg
     1021 ccatccagtt cttcgagctc gatattgggc tcgggccatc ggccccgggc gatcgccggc
     1081 gagtcgggga actcgcgtgc gtagtccagc ggcttcagtg tcacgcgaag gcttgcggtg
     1141 cccgccaggc ggatgttcag ttgtgccggc tttcctgcag acatgatggt cccggcggga
     1201 actgatgccg ataccagcag gagaatcggg acgagaacca gcaagcgggg gttagttgag
     1261 aaaatcactg gcaaaaaatc attggttgtc gtcaaaccct ggttggcacg tagagcagtt
     1321 agccggaaca acggcaggca ctttgcctca tatcggcacc gcggtgccag tcaccacgat
     1381 gcctccgcta accggaatgt cgacggacaa ccggctcggg cgtcccatca cttcgccctg
     1441 ccgtacaagt acggaagcgg gcggtgacac ggacctcaag gctcgcaaat atcccccgag
     1501 ggcagcggct gccgcgcccg ttgccggatc ctcgacgacg cctccgaccg ggaacgggtt
     1561 gcgcgaatga aaaaccagtt cgctctcccg ccagaccagt tgcagggtag tgagcccgtc
     1621 tcgcagcatc aatgctttca gctggtcgaa gtggtagtcg aggtggctca gccggccgga
     1681 ttccgccgcg gccagcacga gatgccatgc tcccgcgtag gcgcgcgccg gtggaatgga
     1741 atcatccagc tggtcccgtc tccaattcag cgcctgcagc gcgttcgtga cgagatcgtc
     1801 cgctgcgggt tcgtacctcg gctcgacgga tgtcagtgat gcttccctga tcccattgcg
     1861 cgatcgtacc gtgaccggca cctggccgac ccgggtatcc agcgtatagg ttccgtctcc
     1921 ctcgacgctg cccaatgcca cgccggtcgc aatagtcgcg tgcccgcaaa aggacacttc
     1981 tgcctcggga ctgaaatagc gcaccgtgcg gcgctgcccg gcgcccggtg cgacgaacgc
     2041 tgtttccgaa tagccgacgc tcgcagcgat gcgttgcatg acgtcagctg ccggcaacgc
     2101 tttgcctacc cagacgccgg ccgggttgcc cccgtcactg cgatccgaaa aagcggcaag
     2161 cctgtagagt gtgcccgggt ccatattctt cttcctctta ccagttgtgc gagatgccta
     2221 ctgcctggcc ctgaagcgct tcaccttcgc actgttgccg caggtcttca tgctgcacca
     2281 cgttcgcgaa cggttcttcg acagatcgag gaacagccag ccgcatcctt catcggtggg
     2341 gcattccttg acgcggtcca gcccgccatg ttccagcagt tccgcggcct gccatgcgac
     2401 cggaccgaga accgcctcgg gaggcgcacc ctcgaagtcc cacccgcagc tgatctcacc
     2461 gccgtgggcg accaggcggc gccaccgcgc ggtttcgcgg ataacctcgt tgagccggtc
     2521 gagggatcgc tgcggcagcg gcttcctggc ggcgacggca tgaaacagtg cacgcagggc
     2581 gccgcgcagg gcagtcacct tcttcactgc ggcttcctgc cgcttcaggg gattcctgct
     2641 caacgcccgc cggctcccgg ccccgatcat tccagccgcc tggtgccagt cgagaatgtc
     2701 ggtgtaagta cgcaggtact ctgtgcctgg tacgccaccg gtccaggaat tcaccgtgtt
     2761 cacgaagtcg agggcgggat gccctccgac ccggtccagc gctctcagct gtcgtgcctg
     2821 ttcgttcatg gtaaccagta taacagggtt gacaggttac gaaactcatc ctacacttgt
     2881 aaccggtaaa agtgctttag atggttatca aacccgcctt ttcgaggaga accgtcatga
     2941 atccctggga catcgacccg aacctgtacc tgcccctgtt ccgcactata tttcgctcgc
     3001 tgcgatcgtc cttccgacat ctgctcgcgt cgcgagctcg agccaggtcg ctacgcaccg
     3061 ctccgccggc cggctgcagg ggttaggcca tgcatcacag tcgccttggt accctcatga
     3121 tcgattgtct cgatcacgag ttcgacgcga gcttcacctt ctggtcggaa gccctgaacc
     3181 tcgcacctca ccgccgaccg tcacgcaagc agcgctatgc gaccgtgggc aagatagaag
     3241 ggccgctgtt cgtccgcttg cagagggtgg acaacaatcc cggctaccac ctcgatatcg
     3301 aaaccgacga cctgcttgcg gaagtcgctc gcctcaaagg ccatggcgcg cgcaaccagc
     3361 ggcgagtaaa gcgctggtgg gttgccgagg atccctccgg taacgcgttc tgcgtgattc
     3421 gcccggagtc ggatgatttt ccgcgcaacg caaatccctg ggagggcaag tgacatgatc
     3481 atgcgcgagt ggcgaggacg cctgccgcac gacaaggtgg atgagtacct ccagtacctg
     3541 agggtgaccg ggcttaacga atatgccaat acccccggac atcacggaac ctgggtgctg
     3601 ctcgaccggc atctcgatgg caagcccggt atcgcagagt acacattgct gacgctatgg
     3661 gaatcacgag acgctattcg ggcatttgcc ggagacgaca tcgagaaggc gaagtattac
     3721 cccgaagatg agcac
//
LOCUS       DASUOM010000175         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1031000,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000175 DASUOM010000000
VERSION     DASUOM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031000"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFY27_03550"
     CDS             <1..591
                     /locus_tag="VFY27_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX5764614.1"
                     /translation="VGSFVITSSALTMEAFLLAAVVAGVIMRAQGIPFADIMDTGMAG
                     IKGVMPAVIILAFAYALNDLSASLQTASYIVSKTEAWLTPGLLPALTFLIAGTIAFST
                     GTSWGTFAIMMPIAVPIAFSFTGDAVDVLVYATVAAVAGGGVFGDHCSPLSDTSILAS
                     TGAASDHIDHIKTQMPYALLVGTISLSAYLLIGIFI"
     gene            complement(584..2152)
                     /locus_tag="VFY27_03555"
     CDS             complement(584..2152)
                     /locus_tag="VFY27_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764615.1"
                     /translation="MTRRLTQTARCLALLALSALASCDSATAFLPEHPAIDILIENGE
                     VFDGLGGDPVQADVVIVGDSIVFVGDAAFSEEDLARRVATRIDAAGRVVTPGFIDLHS
                     HGDPLETPEFENFLAMGVTTISLGQDGSSPDTVNLEGWLDRVSHKGIGPNLAMFVGHG
                     TLRSLSGIGLAPEPAPEDLATMLSMLDKTLPYTFGMSTGLEYNPGLNARPAELRALAE
                     VVGRHGRVLMSHMRNEDDDQLELSIAELLEQGEYARVHISHLKSVFGKGAARADEILG
                     ILDAARDSGVEITADVYPYTASYTWIGIVFPAWAKTAEQFEVARRERRDELARYLRER
                     VEKRNGPEATLLGTGPYKGKTLADLSFEHEMPFEDVLIDVIGPEGASGAYFVMDEELQ
                     SRLLQRPYVGICSDGSPTGFHPRGHGTFAKIIEDYVVRQGALSLAEAVRKMTSFPAGI
                     LGIDDRGRLQPGSKADVLLFDPARVRARATYPEPLQLAEGFDVVIVNGEIARSDGRLG
                     ELSGRVLRPQASVR"
     gene            complement(2377..2625)
                     /locus_tag="VFY27_03560"
     CDS             complement(2377..2625)
                     /locus_tag="VFY27_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764616.1"
                     /translation="MSEKTCNILRFPLDRSRGIQGCPHCGKRTSVWPIGRLAWAYCDV
                     HAVRWVAADLEAAAKGKLDRQQLRKGLEFLSAFVEVSR"
     gene            2831..3727
                     /locus_tag="VFY27_03565"
     CDS             2831..3727
                     /locus_tag="VFY27_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764617.1"
                     /translation="MNARLLTALAVFAAPPQVTYAQDSQVTFKSTDLGSGLYMLEGQG
                     GFAGGNLGLSTGEDGVVLIDDGLGELASMTLSAVEDIASRPIDFVINTHVHGDHTGGN
                     VALHESGATVIGHENVRRRMVEDDSVSADGSAPAAKEAMPEITFSRSMTFHLNGHEAF
                     VFHVASAHTDGDSVIFFRGANVIHAGDIFFNAIFPFIDLASGGSVAGYIEAMDDILSL
                     AGPDTRIIPGHGALANKAELQAARDMLAGARSKVKALLDDGKSEQDILDENPLADYES
                     WSWNFITTEIMTRTLVRDLQSM"
     gene            3849..4052
                     /locus_tag="VFY27_03570"
     CDS             3849..4052
                     /locus_tag="VFY27_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX5764618.1"
                     /translation="MAIAKVIEISASSTTGFEDAVRDGINRAAQTVHNMRKAWVNEQS
                     VVIRDDNIAEWHVNLRITFVLDD"
     gene            4237..4581
                     /locus_tag="VFY27_03575"
     CDS             4237..4581
                     /locus_tag="VFY27_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5764619.1"
                     /translation="MSAVTLGTTDMARSVTFYESLGFRARVGGKDADFTTFDLGATSL
                     NLIAGELIEATRWWGRVIFYVADVDALYALASMMALKPHAPPRDASWGERFFHITDPD
                     GHELSFARPLKS"
     gene            complement(4570..>4897)
                     /locus_tag="VFY27_03580"
     CDS             complement(4570..>4897)
                     /locus_tag="VFY27_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764620.1"
                     /translation="EVTICSRLRHSVDCLVHPFMFRAVSAAEHPIIGLDAVADDPCSA
                     VVTDRRQRLNRALERIESMLFAKSFNDESLVVVVTAVFTLGHGSNSLQFHGRPAGSTI
                     QDRSTL"
BASE COUNT         1002 a         1512 c         1439 g          944 t
ORIGIN      
        1 gtcggatcgt ttgtcatcac atcatcggca ctgaccatgg aggcgtttct cctcgccgcc
       61 gtcgtggcag gagtgatcat gcgggcacag ggaatcccgt ttgccgacat catggacacg
      121 ggcatggcgg gaatcaaagg agtaatgccc gcggtgatca tcctcgcctt cgcctacgcg
      181 ctgaacgacc tgtcggcttc cctgcagacc gcgagctaca tcgtgtcgaa aacggaagca
      241 tggctgacac cgggcttgtt gccggcgctc acgttcctga ttgccggcac tattgccttc
      301 tcgaccggaa cgtcgtgggg aacgttcgcg atcatgatgc cgatcgcagt gcccattgcc
      361 ttcagcttta cgggcgacgc ggtggatgtc ctggtatacg ccacggtagc cgcggtcgca
      421 ggcggcggag tgttcggcga tcactgttca cccttgtccg acacctctat tctcgcttcc
      481 accggcgcgg cctcggatca tatcgaccac atcaagacgc agatgcccta cgcgctgctg
      541 gtgggaacga tcagcctgtc agcttacctg ctgatcggca tcttcatctg acggaggctt
      601 gcgggcgcag cacgcggccg gacagttcgc ccaggcggcc gtcggagcgt gcgatttcgc
      661 cgttcacgat aacgacgtcg aagccctccg ccagctgcag cggctcggga taggttgccc
      721 gcgcgcgtac ccgggccgga tcgaacaaca agacgtcggc tttactgccc ggttgaaggc
      781 gcccacgatc atcgatgccc aggatgccgg cggggaacga agtcatcttt cgcacggcct
      841 cggcgagcga taaagcgccc tgcctgacca cgtaatcctc gatgattttt gcaaacgttc
      901 catggccccg cggatgaaag cccgtcggac tgccgtcgga gcaaatgccg acgtatggcc
      961 gctgcagcaa gcgcgactgt aattcctcgt ccataacgaa gtaggcaccc gaagctccct
     1021 cgggcccgat cacgtcgatc agcacgtcct cgaacggcat ctcatgctcg aacgaaaggt
     1081 cagcgagggt tttgcccttg tacggcccgg ttccgagcaa ggtagcctcg ggaccgttcc
     1141 gtttctcgac cctctcccgc aggtaccttg ccaattcatc ccggcgctcg cgccgggcca
     1201 cctcgaattg ttcagccgtt ttcgcccagg ccggaaacac gatgcctatc caggtgtagc
     1261 ttgccgtgta cggatagaca tccgccgtta tctcgactcc ggaatcccgc gcagcatcga
     1321 gtattccgag gatctcatcg gcgcgcgctg cccccttgcc gaacacggat ttgagatggg
     1381 aaatgtgcac acgtgcgtat tcgccttgct ccagcaactc cgcgatcgac agttcgagct
     1441 ggtcgtcgtc ttcgttcctc atatggctca tgaggacacg cccatgcctg ccgaccactt
     1501 ccgccaatgc gcgaagttct gccggcctcg cgttcaggcc gggattgtat tccagcccgg
     1561 tgctcatgcc gaaggtatag ggcaacgttt tgtcgagcat cgacagcatc gtcgcgagat
     1621 cttccggagc gggttccgga gcaaggccga ttcccgacag gctgcgcagc gtgccgtgcc
     1681 cgacaaacat ggccaggttc gggccgatgc ctttgtgcga gacccggtcc agccatcctt
     1741 caagattcac cgtgtccggg ctcgagccgt cctggccaag ggaaatggtc gtgacgccca
     1801 tcgccaggaa attctcgaat tccggcgtct ccagcggatc accgtgcgaa tgaaggtcga
     1861 taaaccctgg tgtaaccacc ctgcctgcag cgtctatccg cgtcgcaacg cgccgggcca
     1921 ggtcttcctc gctgaatgcg gcatcgccga cgaacacgat cgagtcaccg acgatgacaa
     1981 cgtccgcctg caccgggtcg cccccgaggc cgtcaaaaac ttcaccgttc tcgatcagga
     2041 tatcgatcgc cgggtgctcg gggagaaacg cagtggcgga atcgcaggac gcgagcgcgg
     2101 ataacgccag cagcgcgagg cagcgcgccg tctgtgtcaa tcgtctggtc aaaggtctcg
     2161 tctccggtag ctttcaatca cccgcaaaag tcgctcccga agcaactcca gagagagtct
     2221 tcgtaactct tgaatagaag actgtaggag cccgccgtgc gggcgagcgc gatacgctct
     2281 ttttcgaccc tggcgagctc gcccgcatgg cgggctccta ccaggagtcc ccgctggggg
     2341 cgactttcct tccgcatcct acaggagacg aggcaactag cgcgagactt cgacaaacgc
     2401 agacagaaac tcgagcccct tgcgcaattg ctgccgatcg agctttccct tcgctgccgc
     2461 ttcgagatct gccgcaaccc atcgcactgc atgcacgtcg caatacgccc aggccagccg
     2521 tccgatcggc cagacactgg tgcgcttgcc gcagtgagga cagccctgga taccgcggct
     2581 gcgatccagc gggaaccgga ggatgttgca ggtcttctct gacatcaagg aatctgtatc
     2641 tgcgttctgc cccggacagc ccggtaatga acgcttcaaa taaccgaaag gcagccagcg
     2701 ggctgtgatg tcgctctcag ccgcaccggg ggctcccgcc ggcctgccca aactgagaca
     2761 ttcgttcatt tgatggcccc ggtttgctag agtcggcaaa gctcaacgcc ggacacggag
     2821 aacaagaata atgaatgcca ggctgcttac ggcgctcgcg gttttcgccg cgccacctca
     2881 ggtcacgtac gcccaggata gccaggtcac gttcaaatcg acggaccttg gttcaggcct
     2941 ctacatgctg gaaggtcagg gaggattcgc cggcgggaac ctgggcctgt ccaccggcga
     3001 ggacggcgtc gtgctgatcg atgacggact gggcgaactc gcatcgatga cgctttcagc
     3061 tgtcgaggac atcgccagtc gaccgataga cttcgtcatc aacacccacg tccatggaga
     3121 ccacaccggc ggcaacgtgg cattgcacga gtccggcgcc accgtcatcg ggcacgaaaa
     3181 cgtcaggcgg cggatggtcg aagatgattc cgtcagcgcc gacggcagcg caccggctgc
     3241 aaaagaggcg atgcctgaaa ttaccttctc gcgctccatg acgttccacc tgaacgggca
     3301 tgaagctttc gtgttccatg ttgccagcgc ccacacggac ggcgattcgg tcatcttctt
     3361 tcgcggcgcg aacgtaatcc atgcgggcga catctttttc aatgcaatct ttcccttcat
     3421 cgacctcgcc tccgggggga gcgtggcagg ttacatcgag gcgatggacg acatcctttc
     3481 gctggcagga cccgacaccc ggatcattcc cggccatggg gcgctcgcca acaaagctga
     3541 actgcaagcc gcaagggaca tgctcgccgg cgcccggagc aaggtgaaag cgctgctcga
     3601 cgatggcaag tcggaacagg acatcctcga tgagaatccg ctggcggact atgagagctg
     3661 gagctggaac ttcatcacca ccgagattat gacccgcaca ctcgttcgcg acttgcagtc
     3721 aatgtaggag ctcgtcatgc gggcgagttc gatacaggtt tgaatacact ggctcgccgg
     3781 tcctcgacaa ccaatggcat agaacttgct tgaaacgaag ctccgacccg acgcaacagg
     3841 agcacagcat ggcgatcgca aaggtcatcg aaatctcagc aagctcgacc accggattcg
     3901 aggatgcggt tcgtgacggc atcaaccggg ccgcccagac cgtgcacaac atgaggaagg
     3961 catgggtgaa cgaacaaagc gtggtcatcc gcgacgacaa catcgccgaa tggcacgtta
     4021 acctgagaat taccttcgtc ctcgacgatt aagttcttgt aggagcccgc catgcgggcg
     4081 agtgcgatgg acgttggtaa gaccagccgt atgacgagcg cctggtcgct cggctaaaac
     4141 aatcgatttg taaaaattgc acgacgccga caccggcctg caaagattac aaggcagctt
     4201 tctatcagcc cggaaagacc aatcgccatt gaacggatca gcgcggtaac cctcggcacg
     4261 accgatatgg cccggtcggt tacgttttac gagtccctcg ggttcagggc gcgggtcgga
     4321 gggaaggatg cggacttcac tacctttgat ctcggcgcga cgagcctgaa cctgatcgca
     4381 ggcgagctga tcgaggcgac gcgctggtgg ggtcgcgtga ttttttacgt cgccgatgtg
     4441 gacgccctgt acgcgctggc ttcgatgatg gcactgaagc cccacgcgcc gccgcgcgat
     4501 gcgtcctggg gagaaaggtt cttccacatc acggatcccg acggtcatga gctgagtttc
     4561 gcgcgcccgc taaagagttg aacgatcctg gatagttgaa ccggccggac ggccatgaaa
     4621 ttgtaaggag ttagagccat gccccaatgt gaaaactgcg gtaacgacta cgacaaggct
     4681 ttcgtcgtta aagcttttgg cgaagagcat actttcgatt ctttcgagtg cgcgattcaa
     4741 tcgttggcgc cggtctgtga ccactgcgga acacggatca tcggccacgg catcgagtcc
     4801 gatgatcgga tgttctgctg cgctcactgc gcggaacatg aaggggtgga cgaggcagtc
     4861 gaccgagtgt cgtagccgcg agcatatcgt cacttcg
//
LOCUS       DASUOM010000176         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_152334,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000176 DASUOM010000000
VERSION     DASUOM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152334"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VFY27_03585"
     CDS             complement(<1..1551)
                     /locus_tag="VFY27_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX5764621.1"
                     /translation="MTPGFNATAVWVFAAFIIASLLVTWWAAVRTRSAADFYTAGGKI
                     TDLQNGLALAGDFMSAATFLGLTGLAFMGGADALLFAIGGTVGWAVILFIFADRLRNL
                     GRYTFADVVAFRLHPEQLRILAAVGTLIVAIPYLIAQMVGAGGLFQALFNLPYGIAVL
                     IVGTLMTVYVAFGGMLATTWVQVIKAVLLLTGGTALAMMALLPFGFDLNAMFSRAAEI
                     HPIGERILAPGGLYGDPVSVISLALAFIGGTAGLPHVLMRFFTVPDARQARKSIGYAV
                     TFIGYFMGLTVIIGFAAIVLLAGETQYLDASGRIAGGANMTAVYLARETGGDAFFGFI
                     SAVAFATILAVVSGILLSASASVAHDLYARVIRRGNASDRQEMIVSRIATVGIGAIAI
                     GLSILFRNLNIGVISALVLAVAASVNFPVLILALFWPGLTTRGAIVGGSTGLVVVILL
                     TVLSPTIWVSVLGHEEAIYPYAYPTLFSMLAAFGVTIAVSMADRSPRAAVDRAAYPAQ
                     LVRSELGQP"
     gene            complement(1548..1847)
                     /locus_tag="VFY27_03590"
     CDS             complement(1548..1847)
                     /locus_tag="VFY27_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX5764622.1"
                     /translation="MSERGELLNDADFRRLLQRRSRLRWWLAGFLTAAYLGYGLAGIY
                     TPALLAKSLPNSSITLSIALGYGIIALGIACSIGYVWQVNKIIAPLQKKIAGEWR"
     gene            complement(1899..>2735)
                     /locus_tag="VFY27_03595"
     CDS             complement(1899..>2735)
                     /locus_tag="VFY27_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5764623.1"
                     /translation="GLINVISGDDELGAVLSQHADVDKLVFTGSIATGKKIMSSAAST
                     LKPLTLELGGNDPAIMLPDADPEKFAEGLFWGSMINSGQTCGAIKRLYVHDSLFDRTC
                     DALVAFSRKIPMGDGMDESNLLGPLQNERQFKRVVELVEDAKAHGATILSGGEPVGGN
                     NWFYPVTFVSDIQDGTRLVDEEQFGPVLPLIRYSDVDDAVQRANSLEFGLDASVWGTD
                     RNQTQRIAERLEAGTVYVNKHAEIAPHVPFGGIKCSGLGVEFGEEGLAAYTSIKVINA
                     AA"
BASE COUNT          581 a          844 c          840 g          470 t
ORIGIN      
        1 cggctgaccg agctcgctgc gtacgagctg ggcgggatac gcggctcgat cgacagcggc
       61 gcgcgggctg cgatctgcca tggaaacggc gattgttacg ccgaaggcgg ccaacatgga
      121 aaacagcgtc gggtatgcgt aaggatagat cgcctcctcg tgacccagta cgctgaccca
      181 gatggtcgga ctgaggactg tcaacaggat gaccacgaca agcccggtcg agccgccgac
      241 gattgctcca cgcgtcgtga gccctggcca gaacagcgcc aatatcagca ccggaaagtt
      301 cacgctggca gcgaccgcca ggaccagcgc agaaatgacg ccgatgttga ggttcctgaa
      361 aaggatgctg agaccaatag cgattgcgcc gattccgact gtcgcgatgc gagacacgat
      421 catttcctgg cgatccgagg cattgcctcg gcggatgaca cgtgcataga ggtcatgagc
      481 cacgctcgcg gacgcggaca ggaggatgcc cgagacgacg gcgaggatgg ttgcgaatgc
      541 caccgcggat ataaagccaa agaaggcgtc gccgcccgtc tcgcgggcaa ggtataccgc
      601 cgtcatattc gcgccgcccg cgatgcgccc gctggcgtcc agatactgag tttctccggc
      661 cagcagcacg atggcggcga aaccgatgat cacggtcaac cccatgaagt atcctatgaa
      721 ggttacggcg taacctattg acttccttgc ctgtcgtgca tcgggtactg tgaagaatcg
      781 catcaggacg tgcggcagtc cggcggtgcc gccaatgaag gccaatgcca atgagatgac
      841 gctcaccgga tctccataca ggccgcccgg cgcgagtatg cgctcgccga tggggtgaat
      901 ctcggcagca cgcgaaaaca tcgcattcag gtcgaagccg aacggcagca gcgccatcat
      961 tgcaagcgca gtgcctccgg tcagcaacag cactgccttg atcacctgca cccaggtcgt
     1021 cgcgagcatg ccgccgaacg ctacgtaaac cgtcatcagg gtgccgacta tcagcacggc
     1081 aatcccgtat ggaaggttga acagtgcctg gaaaagaccg cccgcgccga ccatctgtgc
     1141 gatgagatat ggaatggcaa cgatcagggt acccaccgct gccaggatgc gcagttgctc
     1201 ggggtgaagg cggaacgcca ctacatcggc aaaggtgtag cgacccaggt tgcgcaaccg
     1261 atcggcaaag ataaacagga tcacagccca gccgaccgtg ccgccgatag cgaacagcaa
     1321 ggcatccgca ccgcccatga atgcgagacc ggtgagcccg agaaacgtcg ccgccgacat
     1381 gaagtcgcct gcgagggcga gcccattctg caagtcggtg attttgcctc cggctgtgta
     1441 gaaatcggcg gcggagcggg ttctaactgc cgcccaccag gtcaccagca aactggcgat
     1501 gatgaacgcg gcgaacaccc agaccgccgt cgcgttgaag ccgggcgtca acgccactct
     1561 ccggcgatct tcttctgcag gggcgcaatg attttattca cctgccacac gtagccgatc
     1621 gagcaggcaa tgccgagagc gatgatgccg tagcccaagg cgatgcttaa ggtgatggac
     1681 gagttgggca gcgacttcgc caggagtgcc ggggtgtaaa tgccagcgag gccataaccg
     1741 aggtaagcag cggtgaggaa gccagcgagc caccagcgga ggcggctgcg acgctgcaac
     1801 agccgcctga aatcggcatc gttcagcagt tcgccgcgct cgctcaccgc ggacccggct
     1861 tgctaccgat tgtcatcgcc ggcgcacggc aatccgcttc acgctgccgc attgataacc
     1921 ttgatggacg tataggcagc gaggccttct tcgccgaact ctacgccgag gcccgagcac
     1981 ttgatcccgc cgaacggcac gtgcggtgcg atctcggcat gcttgttcac gtacacggtg
     2041 cctgcttcca gtcgttccgc gatccgctgc gtctggttac gatcggtgcc ccagacggat
     2101 gcatcaagac caaattcgag gctgttcgcc cgctgcacgg cgtcgtccac gtcgctatag
     2161 cggatgagcg gcaggaccgg tccgaactgc tcctcgtcca cgaggcgcgt gccatcctgt
     2221 atatcactga cgaaggtaac cgggtagaac cagttgttgc cgccgacagg ctcgccgccg
     2281 gacagtatcg tcgcgccgtg agccttcgca tcttcaacga gttcgacgac gcgcttgaac
     2341 tggcgctcgt tctgcaaagg cccgagcagg ttcgactcgt ccataccgtc gcccatagga
     2401 atcttgcggc tgaatgcgac cagggcgtcg caggtccggt cgaacaggct gtcgtggaca
     2461 tacagccgtt tgatggcacc gcaggtctgc cccgaattga tcatcgaacc ccagaaaagg
     2521 ccttccgcga atttctccgg gtcggcatcc ggcagcatga tcgccgggtc gttgccaccg
     2581 agttcgagcg tcagcggttt gagcgtcgac gcggcactcg acatgatctt cttccccgtg
     2641 gcgatcgagc cggtgaagac cagcttatcc acgtccgcat gctggctcag tacggcgccg
     2701 agttcgtcgt cgccggatat gacgttgatg agacc
//
LOCUS       DASUOM010000177         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1338588,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000177 DASUOM010000000
VERSION     DASUOM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338588"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFY27_03600"
     CDS             <1..908
                     /locus_tag="VFY27_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5764624.1"
                     /translation="LILMQEQALGAEADQFDIIHSHLDFLSFPMSRRCPTPVLTTLHG
                     RLDLPELVPIFREYADMQLVSISNAQRQPLPHANWQATVYHGLPDLYEYHPTPGKYLA
                     YLGRICPEKRPDHAIEIAKRVGIPLRIAAKVDPVDREYFETQIAPLLNHPLVEYLGEI
                     NDAEKCDFLGNAAAVLCTYDWPEPFGIVLIEALACGTPVFAYRRGSIPEIIDDGVTGF
                     ICNSLTEMAAKIEQLPLIDRRRCREAFLNRFTVERMVKDYLEVYERMATVPARVHALA
                     NSRSLRAVGRTRLTAGEMITPHATA"
     gene            1256..1711
                     /locus_tag="VFY27_03605"
     CDS             1256..1711
                     /locus_tag="VFY27_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764625.1"
                     /translation="MAYGYSRLEEATFHLKRQRSWIRIVDRRNFEQLTDEQRFQLSGR
                     VADESVVQVGKWLGADSMVLFRIEGPTWRERLLARFHGKMPPFVVSSKIISLESGEVL
                     YHDVVTATPMPVSGEWSDYASDYELRPAMHSALDQALSGAILHLHRSFR"
     gene            complement(1777..>2251)
                     /locus_tag="VFY27_03610"
     CDS             complement(1777..>2251)
                     /locus_tag="VFY27_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764626.1"
                     /translation="MASSSFSDLVPPTTDRVPRHTDQDLNERITRQTEHNVGRYAENP
                     GLIDGRLRELDREWDIERTLEANAATVSLVGLSLGRLVNRRWYILPAVVATFLLQHAV
                     QGWCPPIEFFRRLGVRTQREIDEERYALKLMRGDFHAIDQQSQKPVDQVMEAIRR"
BASE COUNT          431 a          663 c          675 g          482 t
ORIGIN      
        1 cgctcattct tatgcaggaa caggcgctgg gtgcggaagc ggatcaattc gacattatcc
       61 attcccatct ggactttttg agttttccga tgagccggcg ctgtcctacg ccggtgttga
      121 cgacgctgca tggacgtttg gatctccctg agttggtccc catattccgg gagtacgccg
      181 acatgcaact cgtctccatc tcgaatgcgc agcgtcagcc gctgcctcat gcgaactggc
      241 aggcgactgt gtaccacggc ttgccggatc tgtatgagta tcatcctacg ccgggcaaat
      301 atctcgcgta tctcggacgg atttgccccg agaagcggcc ggatcatgcg atcgagatcg
      361 ccaagcgcgt cggcatcccc ctgcggatcg ccgccaaagt cgatcccgtc gatcgcgagt
      421 attttgagac ccagatcgcg ccattactca atcatccact cgtcgagtac ctgggcgaga
      481 tcaatgatgc ggaaaaatgc gatttcctcg ggaacgcggc ggccgtgctg tgcacatatg
      541 actggccgga gccgttcggc atcgtcctga ttgaggcgtt ggcctgtggc acgccggtgt
      601 tcgcctaccg ccgcggctcg attccggaaa tcatcgatga cggcgtgacg ggatttatct
      661 gcaacagttt gacggaaatg gcggcgaaga tcgagcagct gcccctcatt gaccggcgac
      721 ggtgccgcga agcgttcctc aatcgtttca cggtggagcg gatggtcaaa gattatctgg
      781 aggtgtatga gcggatggcg accgttccgg cacgtgtcca cgccttggcg aatagccgat
      841 cgttgcgagc ggtggggcgc acgcgcctca cggcgggcga gatgatcaca ccgcacgcca
      901 cggcctgacg accgtcatgc cctgcaggtg agaggtcagg ggctagaggc tagcccctcg
      961 ccccctcgcc attagtgcct tcgcctacca tcggtcggct ttccatcctt ccgtaggata
     1021 gcaagagcct tgggcgtttc cgtagcatgg tgtcatgacc aacgccccga agcatgcgcg
     1081 atcagtattc tttgcggcca ctgttataat gagtctgtgt ctcgaaaccg gctgccggcc
     1141 tccaacgcct atcgcgctgg atgcaacgcc ccttgagctt acgacgaccg aggttgccgt
     1201 ccctcaacac gttcggcgct tggtcgtgtg gtatcccaaa acgggggaac cggaggtggc
     1261 atacggctac agccgactcg aagaggcgac ctttcacctg aaacggcagc ggtcctggat
     1321 cagaatcgtc gatcggcgca atttcgaaca actgacggac gaacagcgtt ttcagctctc
     1381 aggaagagtc gccgacgaga gtgtggtgca agtagggaag tggctcggcg ccgacagcat
     1441 ggtgctcttc cgcattgagg ggccgacgtg gagggaacgg ttgttggctc gcttccatgg
     1501 aaagatgccc ccgttcgtcg tttccagcaa aatcatttcg ttggaaagcg gcgaggtcct
     1561 ctaccacgat gtggtgaccg caacgccgat gcccgtatcg ggcgagtgga gcgattacgc
     1621 gagcgattac gaattgcgtc ccgccatgca ttcagcgctg gatcaggccc tctccggagc
     1681 cattctgcat cttcatcggt cctttcgctg agccctctcg cgccacgatt ctctcgcgct
     1741 ttctgccaca cagctgaagc cgccactcca acgggatcag cgtcggatgg cctccatgac
     1801 ctgatcaacc ggtttctggg actgttgatc gatggcatgg aaatccccgc gcatgagctt
     1861 gagcgcatac cgttcttcgt cgatttcccg ttgggtccgc acgccgagtc ggcggaagaa
     1921 ctcgatcggt gggcaccatc cctgtacggc atgttggagg aggaaggtcg cgacaacggc
     1981 tggcaggata taccagcgcc gattcaccag gcgtccaagg gaaagcccga cgagcgatac
     2041 ggtggcagca ttggcttcga gcgttcgctc gatgtcccac tcccgatcta attctctgag
     2101 cctcccatcg ataagcccgg ggttttcggc atagcggccg acattgtgtt ctgtctggcg
     2161 ggtgatgcgc tcattgaggt cctgatccgt gtgccgagga accctgtcgg ttgtcggcgg
     2221 aaccaggtct gaaaaagagg aactcgccat t
//
LOCUS       DASUOM010000178         8017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_30734,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000178 DASUOM010000000
VERSION     DASUOM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30734"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFY27_03615"
     CDS             complement(<1..997)
                     /locus_tag="VFY27_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764627.1"
                     /translation="MTRARANALILLLLLGSPATVLAVGKGNPSFYDRHVIFDNSPSD
                     GGYESSEGYVVAPSLLELHEGTAPVETGYFVSPPNALRLSWTSAPGGDWRITMEITRR
                     YARPFGFEGGALTFWVFSDSEITADNSPRVYLRDVNQHGTPAITLVKQNERIPAGQWV
                     QVQLPFAEIFDRPIKGTDDSLFALRETLSVSFIQGLDDGEAHTLYVDDIQIRDVLSED
                     TTPPPAPRAVTVRGFERHFDVSWEPVEADDLLSYRVYRSWDGEAFEPVGTQQHSIPRY
                     ADFVGTPPREAMYQVTALDLAGNESDPSMLSAAARTRPFSDDELLTMVQEAHFRYY"
     gene            complement(1153..2442)
                     /locus_tag="VFY27_03620"
     CDS             complement(1153..2442)
                     /locus_tag="VFY27_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5764628.1"
                     /translation="MNSTAIAIEYLRRHPVEAAEQLEAMPDPDLAAAVGGLDPAVMAV
                     PMEYLSPAKALAVFAALPRELKLELLERAAPRLGVTLVAELPERERDDLLGALRPVSR
                     EDLKRLMSFPEDSAGRLMDRSFVGVHAAMTVGEVLKKLQESSVRGARSLFVLARDASV
                     AGRVDIQDLALASPDETISDYIQPVSEIVPAHAGREELVDLLERSRHDSVPVVDTEGR
                     LLGVVRYRSLFRAIEDVASADLQKMVGVSPEERALSVPGFAIKRRLPWLHINLLTAFL
                     AAAVVGLFENLIAQFTALAVLLPVVAGQSGNAGAQALAVTMRGLALREIGMRQWRSVL
                     RKEVVVGLVNGLALAVTCGIAVFLWSRSSGLAVIIGVAMVMSMLAAGIAGALVPIVLT
                     RAGQDPATASSIILTTVTDVAGFFSFLGTAMILSFML"
     gene            complement(2439..3263)
                     /locus_tag="VFY27_03625"
     CDS             complement(2439..3263)
                     /locus_tag="VFY27_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764629.1"
                     /translation="MKDLNPLELAYAREYPGEVAAFLAAQGDEAIARVLAGLPQQESA
                     AVVARLPNGHATRILAEQEDANVAGWLDAASENHALALLLHLDDSRRARVLNLLPRPR
                     MRHALERLLNYPQSSVGALVNAGAIKLDADMLLADAVAVLRADAPGPEQPIWLVDGQS
                     RYIGRLDLGRAMVTASDKLRLSELLVAVRPLRAETSLASALDFPEWQSYLELPVVDRH
                     DHLLGTLSRARLAAALGGGDGADQGLAAGVSELTRQYFRVIAICLGDLLGLPGRTR"
     gene            complement(3268..4032)
                     /locus_tag="VFY27_03630"
     CDS             complement(3268..4032)
                     /locus_tag="VFY27_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764630.1"
                     /translation="MIRDLVQAYPELAAVLVLVLGVVLGKVSETVVRRILALADRLVA
                     RYGGYQRHIVSPAFQQGFALLVFATVVVLAMIISVRLLDIPELTSWLGTVLSYGPPFV
                     LGLFIIGVGYVLGVLLRNLTASVVARGDSNALLPRLVHVAVVSIAVITGLQQVGIDIS
                     FITQLTLIALASLLGALSLSFALGSRQYVSNLLAQSELARYRTGERLRIDQDEGVIVE
                     IYRTGLTLATNDGLVSIPAARLASGPVVRVADQALD"
     gene            4113..5060
                     /locus_tag="VFY27_03635"
     CDS             4113..5060
                     /locus_tag="VFY27_03635"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5764631.1"
                     /translation="MNPHLQHELLVADGHRVPVSAWVPQNPGAPSAILQVLHGLGEHS
                     GRYDRFAKQCCERGLVLIAHDHRGHGSACAPSMLGYFADRGGWDKIVTDALEVQGFAA
                     QQWPGKPLALFGHSMGSFIAQSVAARERGKVSALVLSASTFGARVRLRLGRALTRLVI
                     LRSGGRAKSALLNRLGLGDLNRRFAPNRTAFDWLSRDEAEVDRYVADPLCGFAPSNRL
                     WNDLLGGMIEVGSLRALRRIPENLPVLVTGGQSDPLGGRRGQERLARAFRSTGHAHVD
                     LRLYPGGRHEMLNEINRDEFSHDVLDWIESSVPVKAQAI"
     gene            complement(5082..5897)
                     /locus_tag="VFY27_03640"
     CDS             complement(5082..5897)
                     /locus_tag="VFY27_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5764632.1"
                     /translation="MHILLLGHREIASNVALSMLVMAMPAHRFSIMLSRSVVPISAPP
                     ELIELANLERNLCDQLECLSPQFHDRFLSFDRLAHRTGSPARILAEPNSREGLAALAA
                     MQPDLIISVRYRHILGTAAIAIPRRGVLNLHSGLLPGYRGVMATFWAMLNNETSIGCT
                     LHYIVDGTIDTGPVVGRATVPTDFNRTYLANVLSLYAPGCSMAVEAVTRIESGRARKP
                     IRQTNEGKYFSAPGVNDVARFRQAGRRLFDGGELQQFLHEGNIPVPAAASPSA"
     gene            6011..6598
                     /locus_tag="VFY27_03645"
     CDS             6011..6598
                     /locus_tag="VFY27_03645"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5764633.1"
                     /translation="MMNEVLEALRELIGLGVDGLNSGQMTVRALVIYLTAILLVRIGQ
                     KRFMGKNTAFDVILGIILGSVLSRAITGNAPFVPTVAAGAMLVALHWLFSVASFYWRP
                     FGTLVKGQEKLLIKDGEIQWDNMRRSHIGKHDLEMAIRNTGKVARFEDVAEAHFERSG
                     NISVIPREKEKKLRVIDVAVREGVQNVRIELVEGR"
     gene            complement(6646..>8017)
                     /locus_tag="VFY27_03650"
     CDS             complement(6646..>8017)
                     /locus_tag="VFY27_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter family protein"
                     /protein_id="HEX5764634.1"
                     /translation="LGIDPVSAAYIAVALTFSSTIIIVKLLSDKREIDSLHGRIALGF
                     LIVQDIVVVLGMVTLSAMGVGMSDEAGLAGIGRVMGSGVAVLLFIALFIRYAADPVLR
                     RVARSSELLIIAAVGWALGLGALGDWLGFGMELGGLLAGISLASTQYRDSIASRLSSL
                     RDFLLLFFFLGLGASLDLSMLGEQVGAAMILSLFVLIGNPLIVLAIMGYMGYRKRTGF
                     LAGLTVAQISEFSLIFMGMGISIGHVTQESLGLVTLVGLVTIALSTYMITYSHHLYGL
                     VEHWLDPFERKVAQRELADTERAGEDGTAYEFILFGLGRYGSEIGRRLIAHGHSVLGV
                     DFDPEALETWRKGGHDARFGDATDPEFASGLPLARAKAVISAVPRTAGALTDADPRAA
                     LMYGLREQGFTGKIAVAMYSPSDAATLGRQGADVILTPFSDAAEFAVAALEQILAPHP
                     LTTGDG"
BASE COUNT         1596 a         2475 c         2554 g         1392 t
ORIGIN      
        1 agtaatagcg aaagtgggct tcctgcacca tcgtcagcaa ctcatcgtcg gaaaacgggc
       61 gggtgcgcgc ggcagcggat agcatcgacg gatccgactc gttgcccgcg aggtccagcg
      121 ccgtcacctg gtacatcgcc tcgcgtggag gcgttccgac gaagtccgcg tagcgcggaa
      181 tcgagtgctg ctgcgtgccg accggttcga aggcttcacc gtcccaggag cgataaacgc
      241 ggtaagagag caggtcatcc gcctctacgg gttcccagct cacgtcgaag tgccgttcga
      301 agccgcgcac ggtcaccgcc cggggtgccg gcggcggagt ggtgtcttcg ctgagcacgt
      361 cgcgaatctg gatgtcgtcg acgtacaggg tgtgcgcctc gccgtcgtcc aggccctgga
      421 taaacgacac gctcaaggtc tcgcggagcg cgaacaaact gtcgtcggtg ccctttatcg
      481 gccgatcgaa gatttccgca aatggcagct gcacctgtac ccattgccct gcggggattc
      541 gctcattctg cttgacgagg gttatcgccg gcgttccgtg ctgattgacg tcccttaggt
      601 acacgcgcgg tgaattgtcc gcggtgatct ccgaatccga gaagacccag aacgtgagcg
      661 ctccgccttc gaagccgaag ggccgcgcat agcgccgtgt aatctccatg gtaatgcgcc
      721 agtcgccgcc cggcgccgag gtccacgaga ggcgaagtgc attcggcggg cttacgaaat
      781 agccggtttc caccggggcc gtgccctcat ggagttccag caggctcggt gcgacgacat
      841 agccttcgct ggattcgtag ccgccgtcgg acgggctgtt gtcgaagatg acgtgccggt
      901 cgtagaacga cgggttccct ttgccgaccg caagaacggt ggcggggctg cccaggagga
      961 gcaggaggat caatgcgttt gctcgtgctc tggtcatggt ctgtctcctc aacggcaata
     1021 cgaggcactg gggtgaatag taaagggaga tgaatcaggt tatgccggtg cctctcgccg
     1081 aaacgcccgg tccggaagca catccgatga atacgccgct ggtcagcggc cgtggccttc
     1141 ggtgccctga agctacagca tgaaggacaa aatcatagcg gtgccgagga acgagaagaa
     1201 gcctgcgaca tcggtgacgg tcgtcaggat gatggacgat gccgttgccg ggtcctgccc
     1261 ggcacgagtc aggacgattg gcaccagcgc gcccgcaatt ccggccgcca gcatggacat
     1321 taccatggcc acgccgatga tgaccgccag accagaactg cggctccaga gaaatacggc
     1381 gattccgcag gtaacggcca gcgccaggcc attcacgagc ccaaccacga cttcctttcg
     1441 cagcacggac cgccactggc gcatgccgat ctcgcgaagc gcaagcccgc gcatggtcac
     1501 tgccagcgcc tgtgccccgg cgttgccgga ctggcccgcc acaaccggca gcaggaccgc
     1561 cagtgcggtg aactgcgcga tcaggttttc gaaaaggccc acgaccgcgg ccgccaggaa
     1621 cgcggttaac aggttgatgt gcagccacgg caaacgtcgc ttgatggcga atcccgggac
     1681 agataacgca cgctcttccg gactgacgcc caccatcttc tgcaggtcgg ccgaggcgac
     1741 gtcttcgatc gcgcgaaaga ggctgcggta acggacgacg cccaacaggc ggccttccgt
     1801 atccaccacc gggaccgaat cgtggcgaga gcgctcgagc aggtccacca gctcctccct
     1861 gccggcatga gcaggcacga tctcgctgac gggctggatg tagtcgctga tggtctcgtc
     1921 cggcgatgcg agcgccaggt cctggatgtc cacgcggccc gcaacgctcg catcccgtgc
     1981 aagcacgaac agcgacctgg cgccgcgcac ggaggactcc tgcaatttct tcagtacctc
     2041 accaacagtc attgcggcat gcacgccgac gaaggaacga tccatcagcc gtccggccga
     2101 atcttcgggg aatgacatca gccgcttcag atcctcccgg ctgacgggcc gcagggcccc
     2161 gagcaaatca tcccgttcgc gctccggtaa ttcggcaacg agggtcaccc caagccgggg
     2221 cgctgcacgt tcgagcagct ccaacttcag ttcccgcgga agggctgcaa acaccgcgag
     2281 cgctttggca gggctcaagt actccatcgg caccgccatc acggcgggat cgaggccgcc
     2341 gaccgccgcc gccaggtccg ggtccggcat tgcctccagc tgctcggccg cctcgaccgg
     2401 gtgccgccgc aggtactcga tggcaatggc ggtcgagttc accgggtgcg cccgggaagg
     2461 cccagcaagt cccctaggca gatcgcgatc acccggaagt actgccgcgt caactcgctg
     2521 acgcccgcgg caagcccctg atcggctccg tccccgccgc caagagccgc tgccaggcgg
     2581 gcgcgggaca aggtgccgag caaatgatcg tgacgatcga cgaccggcag ctcgaggtag
     2641 ctctgccact ccggaaagtc gagggcgctg gccaatgacg tctcggctct gagcgggcgc
     2701 actgctacca gcaactcgga aagcctgagc ttgtcggacg cggtcaccat cgcgcgcccc
     2761 aggtcgagac gaccgatgta acggctttgg ccatccacca gccagatggg ttgctccggt
     2821 cccggcgcgt cggcacgcag caccgcgacg gcatcagcca gaagcatgtc tgcgtcgagc
     2881 tttatcgcgc cggcattcac gagagccccg accgacgact gcggatagtt cagcaggcgc
     2941 tccagcgcgt ggcgcatgcg cggcctcggc agcaggttca gcacacgtgc cctgcgggaa
     3001 tcatcgaggt gaagcaagag agcgagggca tggttctcgc tggccgcatc cagccagccc
     3061 gcgacattcg catcctcctg ttccgccagg atgcgcgtgg catggccgtt cggcagccgg
     3121 gcgactacgg cggccgactc ttgctgtggc aatcctgcca ggacccgggc gattgcttcg
     3181 tctccctgcg ccgccaggaa ggcggcgact tcgcctgggt actcacgggc gtaggccagt
     3241 tcgagtgggt tgagatcttt catcgattca atccagggcc tggtcggcca cgcgaaccac
     3301 cgggccactt gccagtcgcg cggccggaat ggaaacgagg ccatcgtttg tcgccagcgt
     3361 gagtcctgtc cgataaattt ccacgattac gccttcgtcc tggtcgatac gcagtctctc
     3421 gcctgttctg tagcgagcaa gctccgactg ggcaagaaga ttggatacgt actgccggga
     3481 accgagtgcg aaggacaagg acagcgcacc gagcagcgag gccaaagcga tcagcgtaag
     3541 ctgcgtgata aaggagatat cgatgccgac ttgctgaaga ccagtgatca cagcaatcga
     3601 tacgaccgcc acatgtacca gccgcggcaa gagagcgttg ctgtcgccac gggcgacaac
     3661 ggaggcggtc aggttccgca acaggacacc cagcacgtag ccgacaccga tgatgaacag
     3721 gccgagaacg aacggaggac cgtatgacag gaccgtcccg agccatgacg tgagttcggg
     3781 tatatcgagc aggcggacgg aaatgatcat cgccaggacg acaaccgtgg caaacaccag
     3841 cagcgcgaag ccctgttgaa aggccggaga gacaatgtgg cgctggtacc cgccataacg
     3901 tgcgacaagc cgatcggcca gggccagtat gcgccgcacg acggtctcgg agacttttcc
     3961 aagtacgaca ccgaggacca gcaccagtac cgcggcaagt tccggataag cttgtaccag
     4021 gtcgcgaatc atgctcttgt tttgccgcct gtcatgttcc gtagctcccg gttgcctccc
     4081 gccgcagaca gcattctgac aggcttctgc gagtgaatcc gcatttgcag catgagctcc
     4141 tggtggcgga tggccaccgc gtaccggtca gcgcctgggt accgcagaat cccggagcgc
     4201 catccgccat tctgcaggtg ctgcacgggc tcggcgagca ttcgggtcgg tacgaccgtt
     4261 tcgcgaagca atgttgtgag cgcgggcttg tgctcattgc tcatgatcat cgaggccacg
     4321 ggtcggcttg cgcgccctcg atgctcggat attttgcgga ccgggggggc tgggacaaga
     4381 tcgtgactga cgcactcgaa gtgcagggat tcgcagcgca acagtggccc ggtaaaccgc
     4441 tggcgctgtt cggccacagc atgggatcgt tcatcgcgca gagtgtcgct gcacgtgagc
     4501 gggggaaggt cagcgccctg gtcctttccg cttctacgtt cggtgcgcgg gtgcggctgc
     4561 gtctcggacg agcgctcacg cgcctggtta tcctgcgctc cgggggccgg gcgaagagtg
     4621 cgctcctcaa tcgcctcggg cttggagacc tgaacaggcg cttcgcgccg aaccgtacgg
     4681 cttttgactg gctatcgcgg gatgaggccg aggtagatcg ctacgtcgcc gatccgctgt
     4741 gcggttttgc gccatcgaac cgtctctgga acgacctgct gggcggcatg atcgaggtcg
     4801 ggtcgttgcg ggccttgcgc cgcatcccgg agaacctgcc ggtcctggtg accggcggcc
     4861 agagcgaccc tctcggcggc cggcgaggtc aggaacgact ggcccgggca ttccgctcta
     4921 caggccatgc ccacgtcgac ttaaggctct acccgggcgg ccgtcacgag atgctgaacg
     4981 aaatcaatcg cgacgagttt agccacgacg tgctggattg gatcgagagc agcgtgccgg
     5041 taaaggcaca ggcgatatag ccgcttgtgc gagggttcgc gtcaggcgga cggagaggcg
     5101 gcagcaggta ccgggatgtt gccctcgtgc aggaattgct gcagctcgcc gccgtcgaac
     5161 aggcgccggc ccgcctgtcg gaaccgggca acatcattga cgccaggggc tgagaagtac
     5221 ttcccttcgt tcgtctgtcg gattggcttt cgtgcccgtc cgctttcgat ccgggtgacg
     5281 gcttcgacag ccatgctgca acccggggca tacagcgaga ggacgttcgc aagataggtg
     5341 cggttgaaat ctgtcggcac tgtggcccgg ccgacaaccg gaccggtatc gatggtgccg
     5401 tcgacgatgt aatgcagcgt gcagccgatg ctcgtctcgt tgttcagcat ggcccagaac
     5461 gtcgccatca ctcccctgta cccgggcaac aatcccgaat gcaggttcag gacgccccgc
     5521 cgcggtattg cgatcgcagc cgttcccagt atgtgccggt aacgcacgga aataatgagg
     5581 tccggctgca tggccgcaag cgctgccagc ccctccctgg agttcggctc tgccaggatc
     5641 cgcgcgggag aaccggtccg gtgcgcgagc cggtcgaacg acaggaagcg gtcgtggaac
     5701 tgcggtgaca ggcattcgag ctgatcacac aggtttcgct ccaggtttgc gagctcgatg
     5761 agttcaggcg gcgcggatat cggcacaacg gaacgcgaca acatgatgga aaaccggtgc
     5821 gccggcattg ccatcaccag catcgacaac gcaacgttgc tcgctatctc gcggtgaccg
     5881 agcaggagta tgtgcatgtg cagtctcgac acagagcaag ccgcttgagt ataccgttcg
     5941 gcatatgcat cgttaccggc atgtggggat cgacttcgac ttctcatgtc catgaagagg
     6001 ggatgctttg atgatgaacg aggttttgga ggctctgcgg gagctgatcg ggctgggcgt
     6061 cgacggactc aattcaggcc agatgaccgt gcgcgcgctc gtcatctacc ttacggcaat
     6121 tctgctcgtc aggatcggcc aaaagcgctt catggggaag aacactgcct tcgatgtgat
     6181 cctcggcatc atcctcggct ccgtcctgag ccgcgccata accgggaatg ctccctttgt
     6241 tccgacggtg gcggccgggg cgatgctggt tgccctccac tggctgttct ccgtagcgtc
     6301 cttctactgg cgtccattcg gcacgttggt gaaaggccag gagaaattgc tcatcaagga
     6361 cggcgaaatc cagtgggaca acatgcgcag gagtcacatc ggcaagcacg acctggagat
     6421 ggcgattcgc aataccggca aggtcgccag gttcgaggat gtggcggagg cgcacttcga
     6481 gcggagcggc aacatcagcg ttattccgcg cgagaaagag aagaagctac gagtcatcga
     6541 cgtagcggta agagaaggtg tgcagaacgt gcgaatagaa ctggtagaag gacgttagta
     6601 cggattctac gtgaggcgcc gctgcaccag gaggtggcag tttattcagc cgtcgcccgt
     6661 agtgagggga tgtggcgcga gtatctgctc gagagcggca acggcgaact ctgcggcatc
     6721 cgaaaacggc gtcagaatca catcggcacc ttgtcgaccg agcgtagcgg cgtccgaagg
     6781 cgagtacatc gcgacggcta tcttgccggt aaagccctgc tcgcggagtc cgtacatgag
     6841 tgcggcacgc gggtctgcgt cggtgagggc gcccgcggtc ctgggcacgg cggagatcac
     6901 tgccttggcc cgtgccagcg gcaggccgct ggcgaattcc gggtctgtag catcgccgaa
     6961 gcgtgcatcg tgtccgccct tgcgccatgt ctcgagtgcc tccgggtcga aatccactcc
     7021 gagcacgctg tgcccgtgtg cgatcagccg ccgcccgatt tcgctgccat agcgtcccaa
     7081 cccgaaaaga atgaactcgt acgcggtccc gtcttctcct gctcgttcag tgtcagcgag
     7141 ctcacgctgt gcaaccttgc gctcaaacgg atcaagccaa tgttccacca gtccgtacag
     7201 gtggtgggag taggtgatca tgtaggtgga tagcgcaatg gtgaccagcc ccacaagggt
     7261 tacgagaccc agtgactcct gcgtcacatg cccgatcgat attcccatcc ccatgaagat
     7321 cagtgagaac tcgctgatct gggcgacggt caagcctgca aggaagccgg tgcgtttccg
     7381 gtagcccatg tagcccatga tcgcgagcac gatcagggga tttccgatca gtacgaacag
     7441 cgacaggatc atcgcagccc ccacctgctc accgagcatg gagagatcga ggcttgcgcc
     7501 aagtcccagg aaaaaaaaca gcaggagaaa atcccgcaag ctgctgaggc gggaggcgat
     7561 ggagtcgcgg tattgggtgg aggccagcga aatacctgcc agcaggccac cgagttccat
     7621 gccgaagccg agccagtcgc ccaatgctcc aaggccgagc gcccacccga cggccgcgat
     7681 aatcagcagc tcggaagagc gcgctacacg gcgcaggacc ggatcggccg catatcgtat
     7741 aaacaatgcg atgaacagca gcaccgcgac gccgctcccc atcacgcgcc cgatgcccgc
     7801 gaggcctgct tcgtcgctca ttccgacacc catggcagaa agagtcacca tgcccagaac
     7861 gacgacgata tcctgcacaa tcaggaagcc taaggcgatg cggccgtgca gggaatcaat
     7921 ctcccgcttg tccgatagca gcttgacgat gatgattgtg ctggagaagg tcaacgcaac
     7981 ggcgatatac gctgcggaaa ccggatcgat gccgaga
//
LOCUS       DASUOM010000179         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_926481,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000179 DASUOM010000000
VERSION     DASUOM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926481"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..391)
                     /locus_tag="VFY27_03655"
     CDS             complement(98..391)
                     /locus_tag="VFY27_03655"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX5764635.1"
                     /translation="MHEPYLELDDLPQSLRNELPEDAQRMYLAIYRRVWEATAMSGER
                     ADQELDQTAHEAAMLEVQRRFEKNDEGQWVQAPIDEAIDPDKLRGGVPDADLE"
     gene            553..1062
                     /locus_tag="VFY27_03660"
     CDS             553..1062
                     /locus_tag="VFY27_03660"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5764636.1"
                     /translation="MNEEDLKHFTDILKTFDTAMLVTQRGRELRSRPMEIVDRTEDGR
                     VWFITSIDSAKLEELTDNPDVNVTMQGDAKFLSISGAVRATRDRDKVDEVWRESFDAW
                     FPEGRDDPTVVLLEIVPTYVEYWDRSGVGVLKSIFASAAELDEDDDAAHGKVNFPKSE
                     SELPHPARH"
     gene            complement(1101..2084)
                     /locus_tag="VFY27_03665"
     CDS             complement(1101..2084)
                     /locus_tag="VFY27_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5764637.1"
                     /translation="MTRIRKILADVNPDRRVERGSGLLSVERARQLAVASGAAVDLFV
                     CDSAGSMVDVALFDTESVASARIEYMQELQNWLESQAAPLREEGGEVTCHVEWHSPRY
                     EAILEKAREVDADLIIRSARRHGRIDRLFFGATDWELIRRAPQLLWLCKKSLDPLSSG
                     LRVLAAVDPSHPEEKKAGLDRKLVEVAGDLVNLFGGSLHLFHAYRPGVMVAPIASATH
                     QGAVPVMRLGSELAAELEKHRKAELRKLAGVAGIPEDHVHLVVGNTSDALDELVMQLE
                     IDVVVAGSVSRGRIERLLIGSTAEAILDDVHCDVVVVKPESFPVDAQQVRA"
     gene            complement(2095..>2292)
                     /locus_tag="VFY27_03670"
     CDS             complement(2095..>2292)
                     /locus_tag="VFY27_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5764638.1"
                     /translation="GRVSSSNPGDGDFIRLYVGTPLAEIERRTILSTLEHYEGDKKKT
                     AEVLGVSLKTLYNRLKQYKST"
BASE COUNT          436 a          741 c          660 g          455 t
ORIGIN      
        1 tcaacaatgt gtaattgtta cgagataccg ctgtctgact gacaaatttc cgccgggttc
       61 ccgcaccgcc cgaacgcacc gtccggggaa ggagtggtca ctccaggtcg gcgtccggca
      121 cgccgccccg cagcttgtcg gggtcgatgg cttcatcaat cggcgcctgc acccattgcc
      181 cctcgtcgtt cttctcgaac cgacgctgca cctccagcat cgcggcctcg tgcgccgtct
      241 ggtccaactc ctggtcggct ctctcgccgc tcattgcggt ggcttcccat accctccggt
      301 agatcgcaag gtacatgcgc tgagcgtctt ccggcagctc attgcgtaac gactgcggca
      361 gatcgtcaag ttccaggtaa ggctcgtgca tcgctttcct tcagaatgcg caaaagaatg
      421 tgcggttttt cgctttttgc aagtagcatg ccacgagttt ccagcgttct cgtccctccc
      481 ggacactggc acgacgcttg cagcgaagac cccaggcgcc aggcacatca acagagacta
      541 cgggtaatcc caatgaatga agaagacctg aagcacttca ccgatattct gaagacattc
      601 gataccgcga tgctggtgac gcagcgcggc cgggaactgc gctcccgtcc aatggaaatc
      661 gtggaccgca ccgaggacgg gcgcgtctgg ttcatcacga gcatcgacag cgcgaagctg
      721 gaggagctta ccgacaatcc ggatgtgaac gtgacgatgc agggcgacgc caaattcctt
      781 tcgatctccg gtgccgttcg cgcgacgcgt gaccgcgaca aggtggacga agtgtggagg
      841 gagtccttcg acgcctggtt tccggagggt cgcgacgatc cgaccgtcgt gctgctcgag
      901 atcgtaccga cttacgtcga gtactgggat cgttccgggg tgggcgtact gaagtcgata
      961 ttcgcttccg cagcagaact ggatgaggac gacgacgctg cgcatgggaa ggtgaacttc
     1021 ccgaaatcag agagcgagct accgcacccg gcgcgtcact agcgcgtaga ccggtttggt
     1081 gggcagtccc ccccgccgca ctatgcacgg acctgctgcg cgtccaccgg gaagctttcc
     1141 ggcttcacta cgacgacgtc acaatgcacg tcatccagaa tcgcctcggc ggtactgccg
     1201 atcagcagtc gttcgatacg gccacgcgat accgagcccg cgacgacgac gtcgatttcc
     1261 agctgcatca cgagttcatc gagcgcgtcg ctggtattgc ccactaccag gtgcacgtgg
     1321 tcttcgggga tgcctgcgac cccggcgagt ttccgcagct cggcctttcg gtgcttttcg
     1381 agctccgcgg caagttcgct accgagccgc atgacaggta ccgcaccctg gtgcgttgcg
     1441 gaagcgatcg gggcaaccat cactccgggc cggtaggcat ggaacaggtg tagcgagccg
     1501 ccaaacaggt ttaccaggtc gccggccacc tcgaccagct tccggtccag tcccgctttc
     1561 ttctcctctg gatgggaagg gtcgacggct gcgagtacgc gcaggccgct gctcaatgga
     1621 tccagcgact ttttacaaag ccacaggagc tgcggcgcac gcctgatcag ctcccagtcg
     1681 gtggcgccga agaacaggcg atcaatcctg ccatgccgcc ttgccgaccg gataatgaga
     1741 tccgcgtcca cctcccgggc tttctcgagg atcgcctcat accgtggaga atgccactcg
     1801 acatgacagg tcacctcgcc gccttcctcg cgaagcggcg ctgcctgcga ctccagccag
     1861 ttctgcaatt cctgcatgta ctctatgcgg gcgctggcaa cgctctccgt gtcgaacagc
     1921 gcgacgtcaa ccatcgatcc ggcggaatcg cacacgaaca ggtcgaccgc agctccactt
     1981 gctacggcaa gctgcctcgc ccgttctacc gaaagcaggc ccgagccccg ctcaacccgc
     2041 cgatcggggt tcacatccgc gagtatcttt ctgatccggg tcatgccggc cgcgtcatgt
     2101 gctcttgtac tgcttcagcc tgttatagag cgtcttcaag ctgacgccta aaacctcggc
     2161 tgtctttttc ttatccccct cgtaatgttc gagcgtcgag agaatggtac gccgttcgat
     2221 ctcggcaagc ggcgtaccga cgtagagccg gatgaagtcg ccgtcaccgg gattggagga
     2281 ggaaaccctc cc
//
LOCUS       DASUOM010000180         7897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_598074,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000180 DASUOM010000000
VERSION     DASUOM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598074"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFY27_03675"
     CDS             complement(<1..635)
                     /locus_tag="VFY27_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764639.1"
                     /translation="MRFIFGLLLLLAWLPSGSAPDRTAYLAAAAGVPIHIFDLQAADV
                     SQSFRSFLSDVPMTNNRPLPAALVDDTVASLSFVKAVTPVDAPSYFVAAYIQKTPLVA
                     VAEAERRRYLRRYGEEVYPQQICPVFLSAANASVPQLLSTAVNLPDAWFVDVEGSAEN
                     FDKLFALTEASHCGFIARELANPTVLPGGLSQKAVRTILEALGDFEATAVFR"
     gene            complement(1134..1808)
                     /locus_tag="VFY27_03680"
     CDS             complement(1134..1808)
                     /locus_tag="VFY27_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX5764640.1"
                     /translation="MSDDSMRPRHALVSALRLLCVRGCILLAMRHAIVAVCILCCSAP
                     AAAQEVVYQEGEVKAAFLYHFATFVQWPETVPRERPFSVAVLGDDEVAAELEKFLPGH
                     QIQGRQMQVRRLSSIEDLADDALLFIGAGENHRLRELISRVEDRSMLVVTEAPGALKD
                     GSMINFQVIDDRVRFEISLLAAQRAGLELSSRLLSAAMSVDTTSAIPAPAVVIFAAAA
                     PASLRL"
     gene            complement(1805..3712)
                     /locus_tag="VFY27_03685"
     CDS             complement(1805..3712)
                     /locus_tag="VFY27_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764641.1"
                     /translation="MKRGITGSVQRKVMVIILITTFAALLVSVIALLSYEARAYRDFV
                     INDLTTQADILAVNSAPALAFNDPEAADANLALLANRTGIVAGAIYTRDGELFSSYQK
                     AGTHVQFPASVQLRDPEVEGDDMKLFHPVLQNNEVLGTVFLQADYELGDRISDYLLIL
                     GAIMAISLLVAALLSLWLQGSVTGPILAVTNVAHKIVRERDFKLRAPRTTEDEIGTLV
                     DAFNAMLGEVGQRQQDLEDSNLKLKQESDERRNAETALRLADRRKDEFLATLAHELRN
                     PLAPMVNALALLEAPDTPPDTTRHARAIIDRQLSHMVRLVEDLLDVSRISRGKLTIRK
                     ELVDLGSVLKSAVDTVRPLLAAKDQSLSIRLPEAAVHLQADPVRLSQVFSNLLNNATK
                     YTAPGGRVTLAAVVADGEASVTISDSGRGISADTLPVMFEMFVQDTTTDLRGQPGLGV
                     GLALAKRLMELQGGTIEASSEGRGKGSTFTVRMPVEAAPAVVPESEASPGRTDYARYR
                     ILLVDDNVDFATSLSVLLSRLGHAVEVTHDAPEALAVAGGFRPDFAFLDIGLPSMDGY
                     ELARRLLKRLSSDVILVAISGWGQQEDRRRAREAGFEWYLVKPVDLGSIRSILESQAP
                     GQKQVAPGALM"
     gene            3949..5907
                     /locus_tag="VFY27_03690"
     CDS             3949..5907
                     /locus_tag="VFY27_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764642.1"
                     /translation="MAIFIAFPVSPAHAAAELFERTIEELGELRVISVSRRSESLRQA
                     ASSIHVITAEDIRRSGYSTIPEVLRLAPGVEVARDGAHSWTISIRGFTSDLSNKLLVL
                     IDGRSVYSPLFAGVFWDAQDTLLQDIERIEVISGPGGTLWGANAVNGVINIITRSASE
                     TEGTFVELGTGDEEQLSVGVRHGFSVSEDMSARAYLKYADRDASQFPSGEDGVDDMSM
                     TQGGFRLDWERDAATQVTLQGDVYDAELGAMVRGDFTLGTLPGPDTPGNVDISGHNLL
                     ARWRRDLAGGSDIRLQAYYDHTTRNIPGSFNEGRDTLDVDFQHHFLAAERHDIVWGAG
                     FRWTSDQLDNTLFASFVPDERTDQTVSLFFQDRIGLWDDRASLTIGTKLENNDYTDFE
                     VQPNVRATWNVTERHMLWAAISRAVRIPARLNTDLKLTAPVDVPGVDVPLYVMVSGNK
                     GYESEELVATEMGYRAAFTQDLSLDLNLYYNQYDRLQTQEVGDITLVPDPPPGYLVLP
                     ATLANGMEGDTYGGTLVANWQPAAYWRLQFQYAYLEMDLELKPGSVNEDALSIAGNSP
                     THQAAVHAFLQLPYNLDVYAGVRYTDELPALGVPDYTAVDISIGWQATERLRTSLTVH
                     NLNDDTHLEFGGGNLIERSARLVAVFTF"
     gene            5956..6570
                     /locus_tag="VFY27_03695"
     CDS             5956..6570
                     /locus_tag="VFY27_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5764643.1"
                     /translation="MARVSSAEPIPVIPDQRPQSVDACRRCELWKPATQGVPGEGPKH
                     APLMLVGEQPGSEEDLAGVPFVGPAGRMLDRALEAAGISRQEVFVTNAVKHFRFRLRG
                     KRRIHQKPSVGQVEQCRWWLAKELEKIEPAVVVAMGATAARSVLEKPVLIREARGQSL
                     YAGGIVVRVTIHPSYLLRLRDNREQEFERFAEDLQNAARLARRG"
     gene            complement(6589..7803)
                     /gene="clsB"
                     /locus_tag="VFY27_03700"
     CDS             complement(6589..7803)
                     /gene="clsB"
                     /locus_tag="VFY27_03700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX5764644.1"
                     /translation="MSGWTRGNKLKLLENGEEFFPRVFEAIRGARQEVVLETFLWFED
                     AVGLQLLEALVHAAGNGARVQVTADGYGSPGFSPEFLKRLTDAGANIHSFDPRPTLFR
                     IRTNILCRLHRKIVIVDGELAFVGGINFTEEHVRSFGAQSKQDYAVEVTGPVVRDIHA
                     YCRFGRDVHSGSWRRRWRYWLRRFPREVMNPGEGAQVLFAIRDNEEHPTDIENLYRLG
                     IRNAKKSIVIVNAYFFPGWRFIRDLRRAAERGVDVLLVMQGKPDRPVSVGAAAILYND
                     LLSMGIRIVRYTERPLHAKVAVIDDRWSTVGSSNLDPLSLGLNLEANLFILDRDFNAA
                     LRRNLDGLVRQSCEEVSAEGIPRKSLVRRMLLTFGYHLTRRMPTWWRHVPWHEQQVLP
                     MEATEGADRKAG"
BASE COUNT         1507 a         2475 c         2447 g         1468 t
ORIGIN      
        1 cgaaaaacgg cagtggcctc gaaatcgcca agcgcctcga ggatggtccg aactgctttc
       61 tggctgagac ctccaggcag gactgtcggg ttcgcaagtt cccgtgcaat gaaaccgcag
      121 tgggaagctt ctgtgagggc aaagagtttg tcgaaattct ccgcgctgcc ctcgacgtcg
      181 acaaaccagg catccggcag gtttaccgca gtgctgagca gctgcggcac gctcgcgttc
      241 gcagcgctca ggaagacggg acaaatctgc tgcggataga cttcctcgcc gtagcggcgc
      301 aggtagcgtc gccgctccgc ctccgctaca gccaccaggg gagttttctg gatgtacgcc
      361 gcgacgaagt acgagggagc atccacgggg gtgacagcct tcacgaaaga gagtgaggcg
      421 acggtgtcgt cgacgagcgc ggcggggagc ggccggttgt tggtcatcgg cacatcggac
      481 aggaatgagc ggaagctctg ggatacgtcg gcggcctgga gatcgaagat atgtatcggc
      541 accccggcgg cggcggccag gtaagcggtg cggtcaggag cgctgccgga cggcagccag
      601 gccagaagca gcagcagccc gaaaatgaac cgcattgtat ccccccatcg caaacaactg
      661 taccaatcgt tccagaacga cttcgtttta tgagtgctgc cggtcacact ttcgcatgcg
      721 tcgtgctgcc ggcggcgatc tcgcaatccc gccgaagcaa gttgcccgca tttctcccgc
      781 aggcgctgcg gaacctgcag gccaggcgga ctcttctaac cgggtagctg ctgtcgtgct
      841 taccgggtct cgcaaatctt cagcccgggc ttttttagtc ggctgtccgg cagcagattc
      901 cgcggaaaca gaatcgactc gatggttggt ccgaacggcg cgtttagctc ccttctgttt
      961 tggagcgcga cccgcgctgc gacttgtaaa tgtaatttcg ctcaccggta aatctgacag
     1021 cggcgaggta aaagttgcat caaaacccgg ctgcgtcaac cccttgagcg gattttatat
     1081 ctgaattttt cgtggcggac gccacagcgc tttacctgca ctatccgtaa ctgtcaaagc
     1141 cgcagcgatg cgggagcagc cgctgcgaaa ataacaactg ccggagccgg tattgcgctg
     1201 gtggtatcga ccgacatcgc ggccgataac aggcgcgagc tcagctcgag cccggctcgc
     1261 tgcgccgcaa gcaatgaaat ctcgaaccgg acgcggtcgt ctatcacctg gaagttgatc
     1321 atcgagccgt ccttgagcgc ccccggcgct tctgtcacga ccagcatcga tcgatcttca
     1381 accctgctga tcagttcgcg gagcctgtga ttctctccgg cgccgataaa gagcaaggca
     1441 tcatctgcga ggtcctcgat cgagctaagc ctccgcactt gcatctgcct gccctggatt
     1501 tgatgaccgg gaaggaactt ctccagctcg gcagcgactt cgtcgtcgcc gagcaccgct
     1561 accgaaaacg gccgttcgcg aggcactgtc tccggccact gtacgaacgt ggcaaagtga
     1621 tagaggaacg ccgccttcac ttccccttcc tggtagacga cttcctgcgc tgcagctgga
     1681 gcgctgcagc acaggatgca gacggcaacg atggcatgac gcatggcgag caggatgcag
     1741 ccgcggacac acagcaggcg caaggctgaa accaaagcat ggcgggggcg catcgagtcg
     1801 tcactcacat aagtgcaccg ggcgcaacct gcttttgccc gggcgcctgg gactccagaa
     1861 tcgaacgaat gctgccgaga tcgacaggct tcacgaggta ccattcaaat cccgcttccc
     1921 tggcgcgccg ccggtcctcc tgctggcccc agccgctgat tgccacgagt atcacgtcgc
     1981 tcgacaggcg ttttagcaac cgacgcgcaa gctcgtaccc gtccatggat ggcaggccga
     2041 tgtcgaggaa ggcgaagtct ggccgaaacc ctccggcaac tgcaagagcc tccggcgcat
     2101 cgtgggtgac ttcgacggcg tgtccgagcc gcgaaaggag cacggagagg ctggtagcga
     2161 aatccacgtt atcgtcgacg agcagaatcc ggtagcgggc ataatccgtg cgcccggggc
     2221 tggcttccga ttcgggcact acggccggcg ccgcttcgac cggcatgcgc acggtgaacg
     2281 tgctgccctt cccgcggccc tcgctcgaag cctcgatcgt gccgccttga agttccatga
     2341 gacgctttgc caatgcgagg ccgacaccaa gccctggttg tccccgcagg tcggtagtcg
     2401 tgtcctgcac gaacatctcg aacatcaccg ggagcgtgtc ggccgatatg cccctgccgc
     2461 tgtcggagat ggttacgctc gcctcgccgt cggctacgac cgcggcaagg gtaacgcggc
     2521 ctcccggagc agtgtacttg gtcgcgttgt tcaacaggtt cgagaacacc tgtgacagcc
     2581 tcaccggatc cgcctgcaga tgtacggctg cttccggcaa gcggattgat aacgactggt
     2641 ctttcgccgc gagcaggggg cgcacggtat cgactgcact cttcaatacg ctgccaagat
     2701 ccacgagttc cttgcggatc gtcagcttcc cgcgcgagat acgggagaca tccagcagat
     2761 cctcgaccag ccggaccatg tggctcagct gacgatcgat gatggcccgc gcatgcctgg
     2821 tcgtgtcggg cggcgtgtcc ggcgcctcga gcagcgccag cgcattgacc atgggcgcaa
     2881 gcgggttacg caattcatgg gcgagggtcg cgaggaactc atccttgcgc cggtcggcaa
     2941 ggcgcagcgc cgtctcggcg ttgcgcctct cgtccgactc ctgcttgagc ttcaggttgg
     3001 aatcttcgag atcctgctgg cgctgcccca cttcgccgag catggcgttg aaggcatcga
     3061 ccagcgtccc gatctcatct tccgtagtcc gcggcgcacg cagcttgaag tcccgctccc
     3121 tgacgatctt gtgcgcgacg ttggttaccg cgagtatcgg tcccgtgacg cttccctgca
     3181 gccatagcga aagcagtgca gcaacgagaa ggctgatcgc catgatggcg ccgaggatga
     3241 gcaagtagtc acttatgcga tcaccgagtt cgtaatcagc ctgcaggaaa acagtgccca
     3301 gcacctcgtt gttctgcagt acggggtgga acagcttcat gtcgtcgcct tcgacctcgg
     3361 ggtcccgcaa ttgaacgctc gccgggaact gcacgtgagt tcccgccttc tggtagctcg
     3421 agaacagttc tccatccctg gtgtagatcg cgcccgctac tatgcccgta cggttcgcga
     3481 gcaatgcgag gtttgcatcc gccgcctccg gatcgttgaa cgccagcgcc ggcgcggaat
     3541 tgacggcgag gatgtcggcc tgcgtcgtga ggtcgtttat cacgaagtcg cggtaagcac
     3601 gcgcctcgta actcaagaga gcaattaccg acaccagcaa cgcagcgaag gtggtgatga
     3661 ggatgatcac catgaccttg cgttgtactg agccagtaat ccctcttttc atcttctcat
     3721 cgttttaggg ctatactttc ggccgcttcc cctaagtagg cgatagtaaa ggaagcaggc
     3781 cgcgggccca aattaaacat cctcagaagt tataccggac tctctatttt gccgaccccg
     3841 actttcgccg aggattcaaa gcgctgcgcg gcagacgggg tccaccgacc gaccgaacgg
     3901 cgaggaaggc caggcaaaga tggtttcgcg gcgccgttcg cgtcctgcgt ggccattttt
     3961 atcgcatttc ccgtatcgcc ggctcatgcc gccgcggagc tcttcgagcg aactatcgag
     4021 gaactgggag aacttcgggt tatttcggta tcgcggcgca gcgagtcgct gcgccaggct
     4081 gcaagttcca tccacgtcat cacggccgag gacatccgtc gctcaggcta cagcaccatt
     4141 ccggaggtgt tgcggctcgc gccgggcgtg gaagtcgcac gggatggtgc gcactcctgg
     4201 accatctcca tcaggggctt tacgagcgac ctttcgaaca agctgctggt cctgatcgac
     4261 ggccggagcg tctactcgcc gctgttcgct ggtgtcttct gggatgccca ggacacgctg
     4321 ttgcaggaca tcgaacgtat tgaggtgatt tccggtcccg gcggcaccct gtggggcgcc
     4381 aatgccgtca acggcgtcat aaatatcatc acgcgctccg cctccgaaac ggagggcacg
     4441 ttcgtggaac tgggcacggg tgacgaggag cagctgagcg tcggcgtccg tcacggcttc
     4501 agcgtctccg aagacatgtc cgcgcgggca tacctcaagt acgccgatcg cgatgcttcg
     4561 cagttcccgt cgggggagga cggcgtcgac gacatgagca tgacgcaggg tggctttcgc
     4621 ctggattggg agcgggatgc cgccacgcag gtgacactgc agggcgatgt ctacgacgcg
     4681 gaactcgggg cgatggtacg cggcgacttt acgctcggca ccctgccggg tccggacacg
     4741 ccgggaaacg tggacatctc cggccataac ctgctcgcac gctggcggcg cgacctggcc
     4801 ggcggctccg acattcggct gcaggcgtat tacgatcaca ctacccgtaa catccccgga
     4861 agtttcaatg aaggccggga cacgctggac gtcgacttcc agcaccattt cctggccgcc
     4921 gaacgccacg acatcgtatg gggcgctggc tttcgctgga ccagcgacca gctcgacaac
     4981 accctgtttg catcgttcgt gcccgacgag cggacggatc agacggtcag cctgtttttc
     5041 caggaccgga tcggcctctg ggacgatcgc gcttccctca ccattggcac caagctcgaa
     5101 aataacgact acactgattt cgaggttcag ccgaacgtcc gcgcgacgtg gaacgtgacg
     5161 gagcggcaca tgctgtgggc agcaatatcg cgcgccgtgc gcatacccgc ccggttgaac
     5221 acggatctca agctcaccgc gccggtcgat gttcccggag tggacgttcc cctgtacgta
     5281 atggtctcgg gtaataaggg ttatgagtcc gaggaactgg ttgctacgga gatgggatac
     5341 cgggccgcgt tcacacagga cctctcgctg gatctcaacc tgtattacaa ccagtacgac
     5401 cgcctgcaga cccaggaggt cggcgacata accttggtcc ccgatccccc tcccggttac
     5461 ctggtcctgc cggcgacact ggcaaacggc atggagggcg acacttacgg aggcacgctg
     5521 gtggcgaact ggcagccggc ggcgtactgg aggctgcaat tccagtatgc gtacctcgag
     5581 atggacctcg agctgaaacc cggctcggtg aacgaggacg ccctgtccat cgccggcaac
     5641 agccccaccc accaggccgc cgtgcacgcg ttcctgcaac tgccttacaa cctggacgtg
     5701 tacgcgggag tccgctacac cgacgaactg ccggcactcg gtgttcccga ctacaccgcc
     5761 gtcgacatca gcatcggctg gcaggccacg gaacgcttgc gtacctcgct gacggtgcac
     5821 aacctgaacg acgacaccca cctggagttt gggggcggca atctcataga gcgcagtgca
     5881 cgcctggtag ccgtctttac gttctgatcg cacgcttcaa gggcaaaccg acatggcatg
     5941 cgacttgcaa tctgcatggc cagagtgtct tccgccgagc ccatcccggt tatccccgac
     6001 cagcgtccgc agagcgtcga cgcatgtcgc cgctgcgagc tgtggaagcc ggcgacgcag
     6061 ggcgttcctg gcgaagggcc gaagcatgcg cctttgatgc tggtcggtga gcagccgggg
     6121 tcggaggagg accttgccgg cgttcccttc gtcgggccgg ccgggcggat gctggatcgg
     6181 gcccttgaag ccgccggcat ctcccgccag gaggttttcg tcaccaacgc ggtcaagcac
     6241 tttcgcttcc gcttgcgcgg caagaggcgc attcaccaga agccctccgt cgggcaggta
     6301 gagcaatgcc gctggtggct ggcaaaggaa ctggagaaaa tcgagccggc cgtggtagtc
     6361 gccatggggg ccaccgcggc gcgttcggtc ctcgagaagc cggtgctgat ccgggaggcg
     6421 cggggccagt cgctctacgc cggggggatt gtcgttcgcg tgaccatcca tccgtcatac
     6481 ctgctcaggc tccgggacaa ccgcgaacag gaattcgagc gtttcgcgga ggacctgcaa
     6541 aatgcggcgc ggctcgcccg gcgcggctga taccggcgcc aggcatgttc aacctgcctt
     6601 ccggtcggcg ccctctgtcg cttccatcgg tagcacctgc tgctcatgcc agggcacgtg
     6661 ccgccaccac gtcggcatgc gcctcgtaag gtggtaaccg aaggtcagga gcatgcggcg
     6721 gacgagactc ttcctgggga ttccctcggc ggacacttcc tcgcaggact gtcgaaccag
     6781 cccgtcgaga tttcgcctca gcgcggcatt gaagtcacgg tccaggatga acaggtttgc
     6841 ctcgagattc aaaccgaggc tcaggggatc caggttactg gagccgacgg tcgaccaccg
     6901 gtcgtcgatg acggcaacct ttgcgtgcag cggccgctcc gtgtagcgca cgatccggat
     6961 acccatcgac agcaggtcgt tataaagaat ggccgccgct ccgacgctga ccgggcggtc
     7021 cggtttgccc tgcatcacca gcagcacatc gacgccgcgc tcggcggcgc ggcggaggtc
     7081 gcggatgaag cgccagccgg ggaagaagta cgcgttgaca atcacgatgc tcttcttcgc
     7141 gttgcggata ccgaggcggt acaggttttc gatgtcagtc gggtgctcct cgttgtcgcg
     7201 gatggcgaaa agaacctgcg ctccttcccc cgggttcatg acctcgcgag ggaaccggcg
     7261 cagccagtag cgccagcgcc ggcgccagga ccccgaatgc acgtcgcgcc cgaaccggca
     7321 ataagcatgg atgtcccgaa ctacggggcc ggtcacctct acggcgtagt cctgcttgga
     7381 ttgagcgccg aaactgcgca cgtgctcctc ggtgaagttg atgccgccga cgaaggcgag
     7441 ctcgccgtcg acgatgacga tcttccggtg cagccggcac aggatgttgg tgcgtatgcg
     7501 aaaaagcgtc ggccgcggat cgaacgagtg gatattggcg ccggcgtcgg tgagccgctt
     7561 caggaattcg ggcgagaagc ccggggagcc gtagccgtcc gccgtcacct gcacgcgggc
     7621 gccgttccct gctgcatgca cgagcgcctc cagcaactgg agcccgacgg catcctcgaa
     7681 ccacaggaaa gtttcaagca ccacttcctg ccgcgcgccg cggatagctt cgaacacccg
     7741 cgggaagaac tcctccccgt tttcgagcag cttgagcttg ttccccctgg tccagccgct
     7801 catcgggcag gataaatcag gggtgaaagg gtgcgattac cggggctcaa agcagttatt
     7861 ctccgttatt tgctgcaacc ggactaagtg acatgca
//
LOCUS       DASUOM010000181        11767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_97501,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000181 DASUOM010000000
VERSION     DASUOM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97501"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VFY27_03705"
     CDS             <1..101
                     /locus_tag="VFY27_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5764645.1"
                     /translation="RLMDIVNPTDKTVDALMKLELPAGVDVQIKLN"
     gene            602..1255
                     /gene="rplC"
                     /locus_tag="VFY27_03710"
     CDS             602..1255
                     /gene="rplC"
                     /locus_tag="VFY27_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5764646.1"
                     /translation="MTMGLVGRKCGMTRIFTEEGDSIPVTVIEAQPNRITQMKSVEND
                     GYRALQVSTGSRRSSRVRKSQAGHFAAAKVEAGDLMTEFRLQDGEEGEFEIGGEIKVD
                     LFQVGQKVDVIGTTIGKGFAGTVKRHNFSMQDATHGNSLSHRAPGSIGQNQSPGRVFK
                     GKKMSGHMGNVRRTVQNLEVVRVDAERNLILIKGAVPGHSGGRVLVRPAIKARNKGA"
     gene            1258..1863
                     /gene="rplD"
                     /locus_tag="VFY27_03715"
     CDS             1258..1863
                     /gene="rplD"
                     /locus_tag="VFY27_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5764647.1"
                     /translation="MKIKMQGNGSSVEVADSAFGAEFNEPLVHQVVTAFLAGSRAGTK
                     AQKTRAQVRGGGTKPWNQKGTGRARAGSIRSPIWVGGGRAFAATPRDHSQKVNRKMYR
                     GALKSVLSELVRQDRLVVTESLAMEAPKTRELAGKLKELGLKSVLIVNEAFDEKVFLS
                     ARNLPDVGICDASSIDPVVLMRFEKVLVTLPALKMIEERLS"
     gene            1860..2168
                     /gene="rplW"
                     /locus_tag="VFY27_03720"
     CDS             1860..2168
                     /gene="rplW"
                     /locus_tag="VFY27_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5764648.1"
                     /translation="MSAAEHQERLMSVIYVPHLSEKSTIVAEESNQIVFKVRTDATKA
                     EIRQAVELLFEVKVEDVTVLNVRGKIKRHGGNSGRRSAWKKAYVKLAPGSQIDFLGAD
                     "
     gene            2197..3024
                     /gene="rplB"
                     /locus_tag="VFY27_03725"
     CDS             2197..3024
                     /gene="rplB"
                     /locus_tag="VFY27_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5764649.1"
                     /translation="MSLHKAKPTSPGRRFTISVKTPGLHKGKPHSPLVTTKTKTGGRN
                     HSGRITVRHQGGGHKQRYRLVDFKRDKDGVPGIVERLEYDPNRSAHLALIKYADGDRR
                     YILAPKGVEQGAPIVSGDEAPIKPGNSLPIRNIPVGTTVHCVELKPGKGGQLVRSAGA
                     GAQIAAREGAYATVRLRSGEARRIHVECRATVGEVGHGEHNLRKLGKAGAKRWKGVRP
                     TVRGVAMNPVDHPHGGGEGKTSGGRHPVSPWGTPTKGYKTRRNKRTDHMIVRRRKKK"
     gene            3071..3346
                     /gene="rpsS"
                     /locus_tag="VFY27_03730"
     CDS             3071..3346
                     /gene="rpsS"
                     /locus_tag="VFY27_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX5764650.1"
                     /translation="MPRSVRKGPFVDTHLAKKVATASKANDRRPIKTWSRRSMVLPDM
                     VGLTIAVHNGRQHVPVLISENMVGHKLGEFAVTRTYKGHSGDRKTKV"
     gene            3357..3689
                     /gene="rplV"
                     /locus_tag="VFY27_03735"
     CDS             3357..3689
                     /gene="rplV"
                     /locus_tag="VFY27_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5764651.1"
                     /translation="MQVAATLRYARISPQKCRLMADAIRGKNVEEALRTLAFTPKKSA
                     RIVKKVLESAIANAEHNHGADIDELKVSTIEVNEAPTFRRYRARAKGRGARIIKRNSH
                     ITISVGDE"
     gene            3733..4422
                     /gene="rpsC"
                     /locus_tag="VFY27_03740"
     CDS             3733..4422
                     /gene="rpsC"
                     /locus_tag="VFY27_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5764652.1"
                     /translation="MGQKVHPTGIRLGIVKDWSSKWYADSPAYKDYVRMDHELRTFVK
                     KKLKDASVSRIHIERPAKKANITIFTARPGIVIGKKGEDIEKLRAEVAQLLGMGVHDV
                     RINISEVRKPELDAQLVAEGIAQQLERRVMFRRAMKRAVTNTMRVGAEGVKVKVGGRL
                     NGAEIARSEWYREGRVPLHTLRADIDYGFAEAMTTYGVIGVKVWIFKGEVFDKPEAAT
                     AEPQEAAAAAR"
     gene            4444..4857
                     /gene="rplP"
                     /locus_tag="VFY27_03745"
     CDS             4444..4857
                     /gene="rplP"
                     /locus_tag="VFY27_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5764653.1"
                     /translation="MLQPKRTKFRKQMKGRNRGLAERGSDVSFGEYGLKATERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITKKPLEVRQGKGKGNVEYWVCQIQPGRV
                     LYEMEGVTEEIAREAFRLAAAKLPFPTTFVTRQVM"
     gene            4857..5060
                     /gene="rpmC"
                     /locus_tag="VFY27_03750"
     CDS             4857..5060
                     /gene="rpmC"
                     /locus_tag="VFY27_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5764654.1"
                     /translation="MDASELRSMSQEQLGSELLKLRREQFNLRMQQATGQLSRPHEHG
                     RVRKEIARIKTIMNELKRQDDAK"
     gene            5057..5356
                     /gene="rpsQ"
                     /locus_tag="VFY27_03755"
     CDS             5057..5356
                     /gene="rpsQ"
                     /locus_tag="VFY27_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5764655.1"
                     /translation="MNAEEAGSPATGAEDRKTQRVVVGRVVSDKMNKSVSVSIERVIK
                     HPVYGKYVRRTSKVMAHDETNECKTGDKVAIRECRPMSKNKAWQVVDVIERAVQE"
     gene            5408..5776
                     /gene="rplN"
                     /locus_tag="VFY27_03760"
     CDS             5408..5776
                     /gene="rplN"
                     /locus_tag="VFY27_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5764656.1"
                     /translation="MIQMQTVLDAADNSGARRIQCIKVLGGSKRRYAGVGDVIKVSVK
                     DAIPRGKVKKGEVYNAVVVRTRKGVRRKDGSLIRFDGNAAVLLNARLEPIGTRIFGPV
                     TRELRTNQFMKIISLAPEVL"
     gene            5788..6111
                     /gene="rplX"
                     /locus_tag="VFY27_03765"
     CDS             5788..6111
                     /gene="rplX"
                     /locus_tag="VFY27_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5764657.1"
                     /translation="MNKIRKGDEIVVISGKDRGRRGTVLRVLDDERVLVEGINIAKKH
                     QKANPNAMPPVQGGIVDKEMPVAISNVALFNPKTKKGDRVGFRVTEDGGKLRIFKSNN
                     EVVDI"
     gene            6126..6665
                     /gene="rplE"
                     /locus_tag="VFY27_03770"
     CDS             6126..6665
                     /gene="rplE"
                     /locus_tag="VFY27_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5764658.1"
                     /translation="MARLYEQYRAELHDQIKEKLGLKNAMEVPRITKITLNMGVGEAV
                     ADRKVLDHAMSDMERISGQKPVKTLARKSVAGFKIRDGYPIGCKVTLRRERMYEFLDR
                     LVSIAIPRIRDFRGLNGRSFDGQGNYSMGVQEQIIFPEVNYDQVDAVRGMDITISTTA
                     RNAEEGRALLEAFNFPFKK"
     gene            6679..6984
                     /gene="rpsN"
                     /locus_tag="VFY27_03775"
     CDS             6679..6984
                     /gene="rpsN"
                     /locus_tag="VFY27_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX5764659.1"
                     /translation="MAKRSMIERELKRAKLVKRYAKKRAALKATIRDLTVSDEERVRA
                     QEKLNALPRDASPVRQRNRCAITGRPHGYYRKFGLSRSKLREGAMRGEIPGLTKASW"
     gene            6998..7393
                     /gene="rpsH"
                     /locus_tag="VFY27_03780"
     CDS             6998..7393
                     /gene="rpsH"
                     /locus_tag="VFY27_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5764660.1"
                     /translation="MSMTDPISDMLTRIRNGQKARKVSVRMPASNAKTAVARVLKDEG
                     YIADYSTTEDGQKAELTVELKYYEGKPVIENIKRISKPGLRIYRGKEDLPKVLGGLGV
                     AIVSTSAGVMSDRQAREQGIGGEVLCVIS"
     gene            7429..7962
                     /gene="rplF"
                     /locus_tag="VFY27_03785"
     CDS             7429..7962
                     /gene="rplF"
                     /locus_tag="VFY27_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5764661.1"
                     /translation="MSRVAKNPVVMPDGIEFSQSGTTVTLKGKKGALSLDLNSEVQVA
                     QEGNELTVKRRSNSRFASAMSGTTRALLANMVKGVSDGFEKKLELVGVGYRAAAAGKK
                     LNLTLGFSHPVVFVVPEGISIETPSQTEVIIRGADKQQVGHVAAEIRRFRPPEPYKGK
                     GVRYSDERVIIKEAKKK"
     gene            8022..8375
                     /gene="rplR"
                     /locus_tag="VFY27_03790"
     CDS             8022..8375
                     /gene="rplR"
                     /locus_tag="VFY27_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019563616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5764662.1"
                     /translation="MDKKQNRLRRARKARAKIEQLAVNRLSVHRTPRHIYAQVIAPDG
                     GTVLAAASTLEKDVRKGMQSTGNTAAAAIVGARIAEKAKAAGIDTVAFDRSGFRYHGR
                     VKALADAAREAGLKF"
     gene            8403..8909
                     /gene="rpsE"
                     /locus_tag="VFY27_03795"
     CDS             8403..8909
                     /gene="rpsE"
                     /locus_tag="VFY27_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5764663.1"
                     /translation="MARMDQSQSSDDFLEKLVTVNRVAKVVKGGRQFGFTALTVVGDG
                     NGRVGFGYGKAREVPVAIQKAMQAARKNMIKVNLTNETLQYAKKGRHGATYVYMQPAS
                     EGTGIIAGGAMRAVFECAGVRNVLAKSYGSRNPINVVRATIGALSEIHSPKSIAAKRG
                     KKIKEILA"
     gene            8945..9133
                     /gene="rpmD"
                     /locus_tag="VFY27_03800"
     CDS             8945..9133
                     /gene="rpmD"
                     /locus_tag="VFY27_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5764664.1"
                     /translation="MAKPKQLKVTLTKSRYGRLESHRACLTGLGLRRIRHSVTLADTP
                     EIRGMINKVSYLVSVEEI"
     gene            9135..9569
                     /gene="rplO"
                     /locus_tag="VFY27_03805"
     CDS             9135..9569
                     /gene="rplO"
                     /locus_tag="VFY27_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5764665.1"
                     /translation="MRLNSLTPQKGSKKDAKRFGRGHSAGQGKTSGRGQKGQHARSGG
                     YTKVGFEGGQMPLQRRLPKVGFTSLQKSSSVEVRLHELALPEDEVIDMAALRKARLIS
                     AQAEKVKVILSGKLEKAVRLKGVKVTKGARAAIEAAGGSVEE"
     gene            9616..10977
                     /gene="secY"
                     /locus_tag="VFY27_03810"
     CDS             9616..10977
                     /gene="secY"
                     /locus_tag="VFY27_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5764666.1"
                     /translation="MAKRPTGNPADVAKAASKLGELKKRLAFLVGALIVFRVGTFIPV
                     PGVDPAALASFFDQQAGNMLALFNMFSGGALARFGLFALGIMPYISASIIMQMASMIV
                     PSLQQLRKEGEAGKRKITQYTRYGTVGLSMFQSVAAASWLQSQPGVVVNPGPMFLITA
                     CITLVTGTMFLMWLGEQITERGIGNGISMIILAGIVAGLPAAVAGTAELVRNGEMSSG
                     MAIIMLVGAIGATTFVVYMERAQRRITVNYARRQQGRRMYAAQSTHLPFKINMSGVIP
                     PIFASSILLFPATIAQFFGQSANPNAFQGWLQTLGANLGPGQPVYVLLYAAMIVFFCF
                     FYTALVFNSKETADNLKRSGAFVPGIRPGAQTADYIDKVLTRLTFWGALYVAGVCLLP
                     ELLIMFNPSIPFSFGGTSLLILVVVTMDFMSQMQAHAMSHQYEGMMQKANLRNYGRSG
                     QLR"
     gene            11008..11121
                     /gene="rpmJ"
                     /locus_tag="VFY27_03815"
     CDS             11008..11121
                     /gene="rpmJ"
                     /locus_tag="VFY27_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5764667.1"
                     /translation="MKVRASVKKMCRNCKIIRRNGVVRVICTDARHKQRQG"
     gene            11155..11511
                     /gene="rpsM"
                     /locus_tag="VFY27_03820"
     CDS             11155..11511
                     /gene="rpsM"
                     /locus_tag="VFY27_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5764668.1"
                     /translation="MARIAGINIPLNKHVGIALTSIFGIGRSRARQICQVAGVAPETQ
                     VKDLAEPEVANIRSAVAKFVVEGDLRRENAMNIKRLMDLGCYRGIRHRRGLPVRGQRT
                     RTNARTRKGPRRPIKR"
     gene            11560..>11767
                     /gene="rpsK"
                     /locus_tag="VFY27_03825"
     CDS             11560..>11767
                     /gene="rpsK"
                     /locus_tag="VFY27_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5764669.1"
                     /translation="MAEPQKRKKVKKQVVDAIAHIHASFNNTIITITDRQGNALSWAT
                     AGGCGFRGSRKSTPFAAQVASEKAG"
BASE COUNT         2736 a         3256 c         3820 g         1955 t
ORIGIN      
        1 agcgcctgat ggatatcgtg aatccgaccg acaagacggt cgatgcgctg atgaagctgg
       61 aattgcctgc gggcgtggac gtccagatca agctgaactg actggcagca gaacgactgc
      121 aggagaccgc catgtgggcg agttagctga tttcgtatcg cactcgcccg catggcgggc
      181 tcctacatgg tatgggatga attgccgctt ttagcttgcg gcagcgtcga atccggatat
      241 aatatccggc tcgacaggac caggccggtc cgggagtcgg ccaggttcgg aaaacggcgt
      301 cacaaggcct ccaaggcaat gcctgagtcc tgtggtgccc aatcgttagg tcgccagtaa
      361 aaccaaaggc gtactcggca aatcccggga accggtcatt cgaccggagt agggtcagct
      421 cctcaagcac cggaacatca agcatgttcc acgcgttggc gctggccctt tcgcgtgttt
      481 gagagattca gtatcggcca atcgcagtcg atacggtgag agataaccgg aatttagcaa
      541 tagccaacgt aagtggctaa ccaatagtgc tggccaaccg aagtcagcga ggttgattgt
      601 gatgacgatg ggtctggtgg gccgcaagtg cggcatgaca cggattttta cggaagaggg
      661 cgattcgata ccggtaacgg tgatcgaggc gcagccgaac cgtattacgc agatgaaatc
      721 cgtcgagaat gacggctacc gcgcgctgca ggtttccacc ggaagccgca ggtcctcacg
      781 cgtaaggaaa tcccaggcag gccactttgc agcggcgaag gtcgaggccg gcgacctgat
      841 gacggaattc cgcctgcaag acggcgaaga gggtgaattc gagattggtg gcgagatcaa
      901 ggttgacctc ttccaggtcg ggcagaaggt agacgtcatc ggcacaacga tcggcaaggg
      961 ctttgccggc accgtcaagc ggcacaactt ctccatgcag gacgcgactc acggtaactc
     1021 gctgtcgcac cgcgcgccgg gttcgatcgg gcagaaccag tctcccggcc gggtgttcaa
     1081 gggaaagaag atgtcgggcc acatgggcaa cgtgcgccgt acggtgcaga acctcgaagt
     1141 ggttcgcgta gacgcggagc gaaacctcat cctgatcaag ggcgctgtcc cggggcacag
     1201 cggcggccgc gtactcgtgc gtccggcgat caaggcccgg aacaagggag cctgaggatg
     1261 aaaatcaaga tgcagggcaa cggcagcagc gtcgaagtcg ccgacagcgc gttcggcgcc
     1321 gaattcaacg agccgctggt gcaccaggta gtgaccgcgt tcctggccgg cagccgtgcc
     1381 ggaaccaagg cgcagaagac tcgcgcgcag gtacgcggcg gcggcaccaa gccgtggaac
     1441 cagaagggca ccggacgtgc gcgcgccggc agcatccgca gcccaatctg ggtcggcggg
     1501 ggcagggcgt ttgcggcaac gccgcgcgac cacagccaga aggtcaaccg caagatgtat
     1561 cgcggcgcgc tcaagtccgt gctgtcggag ctcgtgcggc aggaccggct cgtcgtgacc
     1621 gagtcgctgg cgatggaagc gccgaagacc agggagctgg ccggcaagct gaaagagctt
     1681 ggcctcaaga gtgtattgat tgtcaacgaa gcgttcgacg agaaggtgtt tctctcggca
     1741 cgcaacctgc cggacgtcgg tatctgcgac gcctcgtcca tcgacccggt ggtgttgatg
     1801 cggttcgaga aagtgctcgt caccctgccg gcgctgaaaa tgattgagga gcgcctgtca
     1861 tgagtgcagc cgagcaccag gaaaggttga tgagcgtgat ctacgtgccg cacctttcgg
     1921 aaaagagcac gatcgtggcc gaagagagca accagatcgt gttcaaggta cgaacggacg
     1981 caaccaaggc cgaaatcaga caggcggtcg aactgctgtt cgaggtcaag gtcgaggatg
     2041 tcacggtgtt gaacgtccgc ggcaagatca agcgtcacgg cgggaattcc ggccgtcgtt
     2101 ctgcgtggaa gaaagcatac gtgaagcttg ctccgggcag ccagatcgac ttcctcggcg
     2161 ccgactgagc cggatccagg aatacgggaa tagatcatgt cactgcataa agcaaaaccg
     2221 acctcaccgg gccgcagatt caccatctcg gtgaagacac ccggcttgca caagggcaag
     2281 ccgcacagtc cgctcgtgac gacgaagacg aagaccggcg gtcgtaacca cagcggtcgc
     2341 attaccgtcc ggcaccaggg cggcggacac aagcagcggt atcggctggt ggacttcaag
     2401 cgcgacaagg acggggttcc gggcatcgtc gagcggctcg agtacgatcc gaaccgcagc
     2461 gcccatctcg cgttgatcaa gtacgcagat ggggatcgcc gctacatcct cgcgccgaag
     2521 ggcgtggagc agggggcacc gatcgtgagc ggcgatgaag cgcccataaa gccgggcaat
     2581 tcgctgccga tcaggaacat tcccgtcggc actaccgtgc attgcgtaga gctgaagccc
     2641 ggcaagggcg gccagctcgt tcgctccgcg ggagccggcg cgcagatagc ggcgcgtgaa
     2701 ggcgcgtatg ccaccgtgcg gctgcgttcc ggcgaagcac ggcgcataca cgtggaatgc
     2761 cgtgcaaccg taggcgaagt cggtcatggc gaacacaacc tgcgcaagct cggcaaggct
     2821 ggcgcgaagc gctggaaagg cgtgcgaccg acagtgcgcg gtgtggcgat gaacccggtc
     2881 gaccatccgc acggcggcgg cgaaggcaaa acctccggag gaagacatcc cgtgagcccg
     2941 tggggtacgc ccacgaaggg atacaagacg cgcaggaaca agcgaacgga tcacatgatc
     3001 gttcgccgcc gcaagaagaa gtagcaaagt tcaggcaaac ctgaagagac gcaatcgagg
     3061 aatatgaaac gtgccacgtt ccgttagaaa aggaccattt gtcgatacgc accttgcgaa
     3121 gaaggttgcg acagcatcca aggcgaacga ccgtcgcccg atcaagacat ggtcgcgccg
     3181 ctccatggtg ttgcccgaca tggtcggcct gaccatcgcc gtccataacg gacggcagca
     3241 tgtgcctgtg ctcatctccg agaacatggt ggggcataag cttggcgaat ttgccgtcac
     3301 ccgtacctac aagggtcatt cgggcgaccg gaaaaccaaa gtttgaggag ccagtgatgc
     3361 aggtagcagc gacacttcga tatgcgcgta tctctccaca gaagtgccgg ctcatggctg
     3421 atgccattcg cggcaagaac gtcgaagagg ctttgcgaac gctggcgttc acgccgaaga
     3481 aaagcgcgcg catcgtcaag aaggtgctgg agtccgccat cgcgaacgcc gagcacaatc
     3541 atggcgcgga cattgacgag ctgaaggtgt ctaccatcga ggtaaacgag gcgccgacgt
     3601 tcaggcgtta ccgcgcccgg gcaaaaggcc gtggcgcacg cattatcaag cgcaatagtc
     3661 acatcacgat ctccgtgggc gacgagtaag gcaccgcacc gggatcgaca attcgacaat
     3721 agagagaatt gcatgggtca aaaggtacat ccaacaggta ttcgcttagg catcgtcaag
     3781 gactggtcgt ccaagtggta tgccgattcg ccggcgtaca aggattacgt gcgtatggac
     3841 cacgaactca ggacgttcgt gaagaaaaag ctgaaggatg catcggtcag ccgaattcac
     3901 atcgagcgtc ccgcgaagaa ggcgaatatc acgatcttca cggcgcgtcc gggaatcgtt
     3961 atcggcaaga agggcgaaga catcgaaaag ctccgggccg aggtcgcgca gttgctgggt
     4021 atgggcgtgc atgacgtgcg catcaacatc agcgaagtgc gcaagccgga actcgatgct
     4081 cagctggtgg ctgaaggcat agcgcaacag ctggagcgcc gcgtcatgtt ccgccgcgct
     4141 atgaagcggg cggtgaccaa cacgatgcgc gtcggtgcgg aaggcgtgaa agtgaaagtc
     4201 ggaggccggt tgaacggtgc agaaatcgca cggtctgagt ggtaccgcga gggccgcgta
     4261 cccctgcaca cgctgcgcgc cgacattgat tacggctttg ccgaagccat gaccacctac
     4321 ggcgtcatcg gcgtcaaggt atggattttc aagggcgaag tgtttgacaa gcccgaggca
     4381 gcaaccgccg agccgcagga agcggcagcc gctgcccgct aaggcccgga ccaggacagg
     4441 acaatgctac agccaaagcg aaccaaattc cggaagcaga tgaagggtcg taaccgcgga
     4501 ctcgcggaac gcggcagcga cgtctccttc ggtgagtatg gcctcaaggc aacagagcgg
     4561 ggccggctga cggcgcgcca gatcgaagca gcgcgacgtg ccatgacgcg ccacatcaag
     4621 cgtggcggca agatctggat ccgcgtgttc cctgacaagc cgattaccaa gaagccgctt
     4681 gaagttcggc agggcaaggg taaggggaac gtcgaatact gggtatgcca gattcagcct
     4741 ggccgcgttc tgtacgagat ggaaggcgtt acggaggaaa tcgcacggga agcgtttcgc
     4801 ctggctgcgg ccaagctgcc gttcccgacg accttcgtaa cccggcaggt gatgtgatgg
     4861 atgcaagcga actgagatcg atgtcgcagg aacagctagg cagtgagttg ctgaagcttc
     4921 gtcgcgagca gttcaacctg cggatgcagc aggccaccgg ccagctgtcg cgcccgcacg
     4981 agcacgggcg ggtacgcaag gagattgcgc ggataaagac gatcatgaac gagctgaaac
     5041 ggcaggatga cgcgaaatga atgcagaaga agcaggtagc ccggcgaccg gcgccgaaga
     5101 caggaagacc cagcgcgtcg tcgtcggacg cgtcgtcagc gacaagatga acaagtcggt
     5161 gtcggtctcc atcgaacgcg tcatcaagca tccggtctac ggaaaatacg ttcgccgcac
     5221 cagcaaggtg atggcgcacg acgagacgaa cgaatgcaag accggcgaca aagtggcgat
     5281 ccgggaatgc cggccgatgt ccaagaacaa ggcatggcag gttgtggatg ttatcgagcg
     5341 ggcggtccag gaataactgg cgcggcgaag gcagtgaagt gaacagattg attgacggaa
     5401 agcagccatg attcagatgc agacggttct tgatgcggcg gacaactccg gcgccaggcg
     5461 tattcagtgc atcaaggtgc tgggcggatc gaagcgtcgc tacgcgggcg taggcgacgt
     5521 gatcaaggta agcgtcaagg atgctattcc gcgcggcaag gtcaagaagg gcgaggtcta
     5581 taacgcggtt gtggttcgca cacgcaaggg cgtccgccgc aaggatggtt cgctgatccg
     5641 tttcgacggc aatgcagcag tgctcctgaa cgcccggctc gagccgatag gcacccgcat
     5701 ctttggaccg gtgacgcgcg agcttcgcac gaaccagttc atgaagatca tttcgctggc
     5761 gcccgaagtt ttgtaaggac gacacagatg aacaagatca gaaagggtga cgagattgtc
     5821 gtcatcagcg ggaaggacag agggcgccgc ggaaccgtgc tgcgcgtgct cgacgatgaa
     5881 cgcgtgctgg tggaaggcat caatatcgcc aagaagcacc agaaggcgaa cccgaacgcc
     5941 atgcctcccg tacagggcgg catcgtggac aaggaaatgc cggtcgcgat ctccaacgtt
     6001 gctttgttca acccgaagac gaagaagggc gaccgcgtgg ggttccgggt tacggaagac
     6061 ggcgggaaac tacgaatttt caagtccaac aacgaagttg tggacatctg aatcagggtt
     6121 cgagaatggc cagattgtac gaacagtatc gcgcagagct gcatgaccag atcaaggaga
     6181 agcttggttt gaagaatgcc atggaagtgc cgcgcatcac gaaaattacg ctcaacatgg
     6241 gcgtcggcga agccgtggcc gatcgcaagg ttctcgacca cgcgatgagc gacatggagc
     6301 gcatctccgg ccagaagccg gtcaagacgc tggccaggaa gtcggtcgca ggattcaaga
     6361 tccgcgacgg ctacccgatc ggctgcaagg tgacgctgcg ccgcgagcgc atgtacgaat
     6421 ttctcgaccg gctggtcagt atcgccatcc cgcgcatccg cgacttccgc ggcctgaacg
     6481 gtcgctcgtt cgacgggcag ggcaactaca gcatgggcgt gcaggaacag attattttcc
     6541 cggaagtgaa ttacgaccag gtagacgcgg ttcgcggcat ggatatcacg atctccacca
     6601 cggcgcgtaa cgctgaagaa gggcgggcac tgctcgaagc gttcaacttc ccattcaaga
     6661 agtaggcaga gaaggaatat ggctaaaagg tcgatgatcg agcgcgagct gaaacgcgcc
     6721 aagctggtga agcgctacgc caagaagcgt gcggcgctga aggcgacgat acgtgatctc
     6781 acggtttcgg acgaagagcg ggtacgggcg caggaaaagc tgaacgcgct gccgcgcgat
     6841 gccagcccgg tgcgccagcg gaaccgttgc gccatcaccg gccggcctca cggttactac
     6901 cggaagttcg gcctgtcccg gagcaagctg agggaaggcg cgatgcgcgg agaaataccc
     6961 gggctgacca aggccagctg gtaggcggag acagaatatg agtatgaccg atcccatttc
     7021 cgacatgctg acgcgcatcc gcaacggcca gaaggctcgc aaggtaagcg tccggatgcc
     7081 ggcatcgaac gcgaagaccg ccgtggccag ggtgctgaag gacgaaggtt acatcgccga
     7141 ctactcgacg accgaggacg gccagaaggc cgaattgacg gtcgaactca agtattacga
     7201 gggcaagcct gtcatcgaga acatcaagcg catcagcaag ccgggcctgc gcatataccg
     7261 cggcaaggaa gacctgccga aggtgctggg cggcctgggc gtcgcgatcg tgtccacctc
     7321 ggcaggcgtg atgagcgatc gccaggctcg cgaacagggc attggcggcg aggttctttg
     7381 cgtcatttcc tgatgcggct ttagcgcagc ggaattagag aaacgattat gtccagagtt
     7441 gcaaaaaacc cggtggtaat gccggacggc attgaattca gccagagcgg cacaaccgta
     7501 acgctcaaag gcaagaaggg cgcgctttcg ctcgacctga actccgaggt ccaggtggcg
     7561 caggaaggca acgagctgac cgtaaaacgg cgctcgaact cgcgcttcgc atccgcgatg
     7621 tccggcacta cccgagcttt gctggcgaac atggtgaagg gcgtatcaga cgggttcgag
     7681 aagaaactcg agctggtcgg cgtcggttac cgcgccgcgg cggcgggtaa gaagctcaac
     7741 ctgacgctcg gtttttcgca cccggtcgtt ttcgttgtgc ccgagggtat cagcatcgag
     7801 acgcccagcc agacggaagt aatcatccgc ggcgcagaca agcagcaggt aggacatgtc
     7861 gccgcggaga tccggcgatt ccggccgccg gagccgtaca agggcaaagg tgtccgctat
     7921 tccgacgaac gagtgatcat caaggaagcg aagaagaagt aacagcagca ccgctgccaa
     7981 atcttcaatt gctttatcga actcaagaga cagtcatgaa actggacaag aaacagaatc
     8041 ggctgcgccg cgcccgcaag gcgcgcgcga agatcgagca actcgcggtg aatcgcctga
     8101 gcgtccaccg gactcccagg cacatctacg cgcaagtcat tgcgccggac ggcggcacgg
     8161 ttctcgctgc ggcatctacg ctcgaaaaag acgtgcggaa gggcatgcaa agcaccggca
     8221 ataccgcggc ggcggcgatt gtcggagcgc gtattgccga aaaagcgaaa gcggcgggaa
     8281 tcgacacggt tgcattcgat cgctcgggtt ttcgttacca cggccgcgta aaagcgctcg
     8341 ctgatgcggc ccgcgaagcc ggcctgaaat tctagaaagc gaagagacga gcaggatcaa
     8401 ctatggcacg aatggatcaa tcacagagca gcgatgactt tctggaaaag ctggtcaccg
     8461 tcaaccgcgt cgcaaaggtc gtcaagggtg gccgccagtt cggttttact gcgctaaccg
     8521 ttgtcgggga tgggaacggg cgcgtcggct tcggttacgg caaggcacgt gaagtgccgg
     8581 tcgcaatcca gaaagcgatg caggccgcgc gcaagaacat gatcaaggtg aacctgacga
     8641 acgaaaccct gcagtacgcg aagaagggcc gccatggcgc cacctatgtg tacatgcagc
     8701 cggcttcgga aggaacgggc atcatcgccg gcggcgcgat gcgcgccgtg ttcgagtgtg
     8761 ccggcgtccg caacgtgctc gcgaagagct acggttcgcg caacccgatc aacgtcgtcc
     8821 gcgctacgat cggcgcgctg tcggagattc attcaccgaa atcgatcgcc gcgaaacgcg
     8881 gcaagaagat caaagagatt ctggcctgag cgccggaggg acctcaagtt tagggacaga
     8941 aacgatggcc aagccaaaac agttgaaagt gacgcttacg aagagccgtt acgggcgcct
     9001 tgaaagccac cgcgcgtgtc tgaccgggct gggactgcgc aggatccggc acagcgtgac
     9061 ccttgccgac acgccggaga tacgcggcat gatcaacaag gtttcgtacc tcgtttctgt
     9121 cgaggagatt tgatatgcgc ctcaattcac tgacgccgca gaaaggttcg aagaaggacg
     9181 cgaagcgttt cgggcgtggg cattcggcag gccagggcaa gacctccggc cgcggccaga
     9241 agggccagca cgcacgatcc ggcggttaca ccaaggtcgg gttcgaaggt ggccagatgc
     9301 ctttgcaacg ccgcctgccg aaggtgggat tcacctccct gcagaagtcg tcttctgttg
     9361 aggttcggct acatgaactg gcgctgccgg aagacgaggt catcgacatg gcagcgctcc
     9421 gcaaggcgcg gctgatctcc gcccaggcgg agaaggtcaa ggtgatcctt tcgggcaagc
     9481 tcgaaaaggc ggtcaggctc aagggcgtga aggtgacgaa gggcgcgcgg gccgctatcg
     9541 aggcggccgg cggcagcgtc gaagagtaga caggacggta acgtccaaga gataccaggt
     9601 aattcgggaa cagaagtggc aaagcggcca acaggcaatc cggcagacgt agcgaaagcg
     9661 gcgagcaaac tcggcgagct caagaagcgc ctggcgtttc tcgtgggcgc gctgatcgtt
     9721 ttccgcgtcg ggacgttcat tcccgtgccc ggggtggatc ccgcggcgct tgcgtcgttc
     9781 ttcgaccagc aggccggcaa catgctggcg ctgttcaaca tgttctcagg aggcgcgctg
     9841 gcacgcttcg gcctgttcgc gctcggcatc atgccgtaca tctctgcatc gatcatcatg
     9901 cagatggcca gcatgatcgt gccgtcactg cagcaactga ggaaagaagg cgaggccggc
     9961 aagcgcaaga tcacgcagta cacccggtat ggaactgtcg gactatcgat gttccagtcg
    10021 gttgccgctg cctcgtggtt gcagagccag cctggagtgg tcgtcaatcc cggcccgatg
    10081 ttcctgatca ccgcctgcat cacgctggtc accggcacga tgttcctgat gtggcttggc
    10141 gagcagatca ccgagcgcgg catcggtaac ggtatttcga tgatcatcct ggccggcatc
    10201 gtggccggct tgcccgcggc agtggcagga accgcggaac tggtccggaa cggcgagatg
    10261 tcctcgggca tggcgatcat catgctggtc ggagcgatag gcgccacgac cttcgttgta
    10321 tacatggagc gcgcgcagcg gcggattacc gtcaattacg cgagacgaca gcagggcagg
    10381 cgaatgtatg ccgcgcaaag cacccatttg cctttcaaga tcaatatgtc gggcgtgatc
    10441 ccgccgattt ttgcatcgag catactgctg ttcccggcga cgatcgcaca gttcttcggc
    10501 cagtcggcga acccgaatgc cttccaggga tggctgcaga ccctgggcgc caatctgggg
    10561 ccgggccagc cggtatatgt gctgctttat gcagcgatga tcgtgttttt ctgctttttc
    10621 tatacagcgc tcgtattcaa ctcgaaggaa acggcggaca acctgaagcg ctcgggggcc
    10681 ttcgtgcctg gcatccggcc cggcgcgcag acggcggatt acatcgacaa ggtgctgacg
    10741 cggctgacgt tctggggtgc gctctacgtg gcaggcgtgt gcctgttgcc ggagctgctg
    10801 atcatgttca acccgagcat cccgttcagt ttcggcggca cgtcgttgct gatcctggtt
    10861 gtcgtaacaa tggatttcat gtcgcagatg caagctcatg caatgagcca tcagtacgag
    10921 ggcatgatgc aaaaggccaa tctgcgcaat tacgggcgca gcggtcagct tcgctgaaga
    10981 atcttcggcg gtttcggagt agagctcatg aaagtcaggg catcggtcaa gaaaatgtgc
    11041 aggaactgca agatcatccg ccgcaatggc gtggtgcggg tgatttgtac cgacgcgcgt
    11101 cataagcagc gtcagggtta aagcagcgcc aggttaaaag gaaaggtact gaaagtggca
    11161 cgtatagcag gcataaacat tccgctgaac aagcacgtcg gcatcgcgct gacgtcgatc
    11221 ttcggtatcg gacgctccag ggcgcggcaa atctgccagg ttgcaggcgt cgcaccggag
    11281 acgcaggtca aggacctggc ggaacccgaa gtcgcgaaca ttcgcagcgc cgtcgccaag
    11341 ttcgtcgtgg aaggcgatct tcgccgggaa aacgcgatga acatcaagcg cttgatggat
    11401 ctcggctgct atcgcggaat ccggcacagg cgcggactgc cggtacgcgg acaacggacc
    11461 cgcacgaacg cgcgcacccg taaagggccg cgccggccga tcaagagata gtcggggcga
    11521 agctgaaccg acagacgcgc aaatttacag gatcacgaaa tggctgaacc gcaaaaacgc
    11581 aagaaggtaa agaagcaggt tgtggatgca atcgcccaca ttcatgcgtc tttcaacaat
    11641 acgattatca cgatcaccga tcggcagggt aacgctctgt cgtgggcgac cgcgggcggc
    11701 tgtggatttc gcggctcgcg caaatcgacc ccgttcgctg cccaggttgc gtcggaaaaa
    11761 gccggac
//
LOCUS       DASUOM010000182         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1505385,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000182 DASUOM010000000
VERSION     DASUOM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505385"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="acnA"
                     /locus_tag="VFY27_03830"
     CDS             <1..972
                     /gene="acnA"
                     /locus_tag="VFY27_03830"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018951456.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX5764670.1"
                     /translation="GDDADGEPVYLRDIWPRQAEIQEFIAKHIDLQMFRTSYASVFAG
                     DENWAGLDVAEGQTYAWDDDSTYVKNPPYFEGMTLDTQPLEDIRGARVLALLGDSVTT
                     DHISPAGSISPGSPAAMYLREMGVEPPDFNSYGSRRGNHEVMMRGTFANIRLRNQLAP
                     GTEGGWTRHQPSDEQMSIYDASLQYQQEGTPLVVIAGSEYGSGSSRDWAAKGTLLLGV
                     KAVIAVSYERIHRSNLIGMGVLPLQFMNGESANSLGLTGKEVFDIAGLGDSQARAVVI
                     VATRPDGDRVQFEARVRIDTPKERDYFEHGGILHYMLRQLATRAQAA"
     gene            1013..2053
                     /locus_tag="VFY27_03835"
     CDS             1013..2053
                     /locus_tag="VFY27_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX5764671.1"
                     /translation="MIFRRRKNGGDGNGAARARPLMADKPAWMKALWAAVAALLVLLI
                     GLGVYWSGEPDVFWVNERPDSERTVVGYATVDTLVRVAETMLDKPGGYLSNDVMPPGV
                     FLDNIPSWEFGVLQQVRDLSRVIRNDYSRSQSQSKEDAHVAVAEPKFSIDNDSWIFPA
                     AESEYRDAITGFARYRDRLSGIGGSDAQFYARADNLREWLAQVEKRLGSLTRRLGDSV
                     AETVINTDLAGERSATSTSELADSVEVRTSWFEIDDIFFEARGTSWALVHFFRAAEFD
                     FADVLADKNAAVSVRQIIRELEASLQPLRSPVVLNGGGFGLFANHSLVMANYLARANA
                     AVINLRELLDQG"
     gene            complement(2127..>2394)
                     /locus_tag="VFY27_03840"
     CDS             complement(2127..>2394)
                     /locus_tag="VFY27_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5764672.1"
                     /translation="YKKCQIRAQRTCWGSRSSSGTVSLNLCLLFLEPAVVRYLMIHEL
                     CHGRHMNHSRRFWSLVSQFEPDYERLDKRLTESWRRVPGWLGIY"
BASE COUNT          463 a          748 c          766 g          417 t
ORIGIN      
        1 ggcgacgacg ccgatggcga gccggtttat cttcgcgaca tctggccgcg ccaggccgag
       61 atccaggagt tcatcgcaaa acacatcgac ttacagatgt tccgcacgag ctacgcgagc
      121 gttttcgccg gcgacgagaa ctgggccggg ctcgacgtgg ccgagggcca gacctacgcc
      181 tgggacgacg actcgaccta cgtcaagaac ccgccctact tcgagggcat gacgctcgat
      241 acgcagccgc tcgaagacat acgaggcgca cgcgtgctgg cactgctggg agactccgtg
      301 acgacggatc acatatcacc ggcgggcagc atctcgcccg gcagcccggc cgctatgtac
      361 ctgcgcgaga tgggtgtcga gccgccggac ttcaattcct acggctcgcg gcgcggcaac
      421 cacgaagtga tgatgcgcgg cactttcgcc aacatccgcc tgcgtaacca gctggcaccc
      481 ggcaccgagg gcggctggac ccggcatcag ccgagcgacg aacagatgtc catctacgat
      541 gcctcgctcc aataccagca ggagggaacg ccactggtgg taatagccgg cagcgaatac
      601 ggctccggct cgtcgagaga ctgggcagcc aaaggcacgc tgttacttgg cgtgaaggcg
      661 gtgattgctg tcagctacga gcgcatccac cggtcgaacc tgatcggcat gggtgtcttg
      721 ccgctgcaat tcatgaacgg cgaaagcgca aacagcctcg ggctcaccgg caaggaagtt
      781 ttcgacattg caggcctcgg cgacagccag gcgcgggcgg tcgtaatagt cgcgacccgg
      841 cccgatggag acagggtgca gttcgaggcg cgcgtgcgca ttgatactcc caaggagcgg
      901 gattacttcg agcacggcgg catactgcat tacatgttgc gccagctggc cacccgcgcg
      961 caggccgcgt gacgacctgg cggaagcggc ccgtgtcaca gccggtgtac cgatgatatt
     1021 caggcgccgg aagaacgggg gcgacgggaa cggagccgcg cgtgcacggc ccctgatggc
     1081 ggacaagccg gcctggatga aggcgctttg ggccgcggtc gcggccctgc tcgtgctcct
     1141 gatcggcctc ggggtctact ggtccggcga accggacgtt ttctgggtca atgagcgtcc
     1201 ggacagcgaa cgtactgtcg tcggctatgc cacggtggat accctcgtca gggtggcgga
     1261 aacgatgctg gacaagcccg gcggttacct gagcaacgac gtcatgccgc caggagtgtt
     1321 tctcgataac attcccagct gggagttcgg cgttctgcaa caggtgcggg acctctcccg
     1381 agtgatccgc aacgactaca gccgcagcca gtcgcagtcg aaggaagacg cgcacgttgc
     1441 agtcgccgaa ccgaagttct ccatcgacaa cgattcctgg attttccctg ccgccgagtc
     1501 cgaataccgg gacgccatta ccggcttcgc gcgttatcgc gacaggcttt caggcatcgg
     1561 cgggtcggac gcgcagttct acgcgcgcgc ggataacctg agggagtggc ttgcccaagt
     1621 cgaaaagcgg cttggcagcc tgacgcgccg cttgggcgac agcgtcgcgg aaacggtgat
     1681 caacaccgat ctcgcgggag aacgctccgc cacttcgact tcagaactcg cggacagtgt
     1741 cgaggttcgt acgtcatggt ttgaaatcga cgacatcttc tttgaagccc gcggcacctc
     1801 ctgggcgctg gtgcacttct ttcgtgccgc agaattcgat ttcgcggacg tgctcgcgga
     1861 caagaacgcc gctgtgagcg tccgccagat cattcgcgaa ctggaagcaa gcctgcaacc
     1921 cttgcgctcg cccgtggtgc tgaacggtgg tggcttcggc ctgttcgcca accactccct
     1981 ggtgatggcg aattacctgg cgcgcgccaa tgcggccgtc ataaacctcc gcgagctcct
     2041 ggatcagggt tgacgccatg cgcgccttac caggcgcagg tttcaagcga gggcgatcgc
     2101 actcgccggc ccagcgggct cctgcatcag tagattccca gccagcccgg gacgcgtcgc
     2161 cagctttcgg tgagcctctt gtccagccgc tcataatcgg gttcgaactg gctgacgagg
     2221 ctccagaagc gcctggaatg attcatgtga cggccgtggc acagctcatg gatcatcagg
     2281 taacgaacga ccgcgggttc gaggaaaagc aggcacaggt tgagactcac cgtgcccgaa
     2341 ctgctgcgac tgccccagca ggttcgctgg gcgcgaatct ggcacttctt gtag
//
LOCUS       DASUOM010000183         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_14418,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000183 DASUOM010000000
VERSION     DASUOM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14418"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFY27_03845"
     CDS             complement(<1..418)
                     /locus_tag="VFY27_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5764673.1"
                     /translation="MLRELYFILRGFLRTSGAMFLFFLRLLQYSPTVLFKRFPLVVQQ
                     IHNAGALSLVIIMICGFFVGAVLGLQGVSNLARFNAEDSVGTLTAFSLLKELGPVITA
                     LLFAGRAGTALASEIGLMKATDQLAAMEMMATNPVRR"
     gene            complement(425..1240)
                     /locus_tag="VFY27_03850"
     CDS             complement(425..1240)
                     /locus_tag="VFY27_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5764674.1"
                     /translation="MSNPTENIVEIRDLDYSRGPKEIFKGLNINIPRGKITAIMGPSG
                     TGKTTLLRLITRQLVADRGHIEVDGIDIAKLNQRDLFRLRQRIGMLFQNGALLTDYNV
                     FENVAFPLREHTKLPNRLIRHIVLTKLHAVGLRGAADMSPAELSGGMARRVALARAMV
                     MDPDILIYDEPFVGLDPISMGVIVRLVRKMNDALGISSIIVSHDVQEISTVADCSFLI
                     SDGKVVASGSPDELRNTSSELVRQFMHGMADGPVAFHYSAPDYAEQLLGRDAE"
     gene            complement(1325..2344)
                     /locus_tag="VFY27_03855"
     CDS             complement(1325..2344)
                     /locus_tag="VFY27_03855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5764675.1"
                     /translation="MNLKELNSVAKAMVANGKGILAADESGGTIKKRFDTIGTESTEE
                     NRRRYREMLFTAEGVANYIGGVILFDETIRQSTSDGVPFARYLNNLGMIAGIKVDKGA
                     KPLAGFPGETITEGLDGLRERLNEYVGLGARFSKWRAVINIGDGIPTDFCLRANAHAL
                     ARYAALCQEAGLVPIVEPEVLMDGDHSIDRCAEVTSRALELVFGELEDHRVELSGIIL
                     KPNMVISGTDARNRAGPEQVARATLDVLKKHVPSEVPGIAFLSGGQSAEEATEHLGVM
                     NQMGPLPWELSFSYGRALQAPALDAWRGKEAQFQAGQKALYKRARLNSLAHLGKYDSQ
                     MESAP"
     gene            2553..3005
                     /locus_tag="VFY27_03860"
     CDS             2553..3005
                     /locus_tag="VFY27_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX5764676.1"
                     /translation="MSKLTDYGTVVLAELASGRAGYVSAAELAEATGLGLPTVSKLLK
                     TLAKAGLVISTRGSHGGYRLARDANHISAAEVIDAFEGPVSITECSASESHCGLEAMC
                     SVGHAWQRINVAIRRALDEISLADLLRANTPTPRFLFAGTPINVERKR"
     gene            3008..>4368
                     /gene="sufB"
                     /locus_tag="VFY27_03865"
     CDS             3008..>4368
                     /gene="sufB"
                     /locus_tag="VFY27_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5764677.1"
                     /translation="MAFIPDKVAEPVGGRNEDDIESLVNRRYKHGFITDIASDSLPPG
                     LDEDVIRAISLRKKEPPFMLEWRLKAYRHWLTMQEPEWAHVHYPKIKYRDISYFSAPK
                     SDEDRPKSLDEVDPKLLETYDKLGIPLHERARLAGVAVDAVFDSVSVATTFKEKLADA
                     GVIFCSFSDAVQNHPELVRKYLGTVVPYRDNFFATLNSAVFSDGSFVYVPKGVRCPME
                     LSTYFRINAANTGQFERTLIIADEGSHVSYLEGCTAPMRDENQLHAAVVELVALKDAE
                     IKYSTVQNWYPGDENGRGGIYNFVTKRGDCRGDNSKIAWTQVETGSAITWKYPSCILR
                     GANSVGEFYSVAVANNMQQADTGTKMIHIGRNTSSTIVSKGISAGRAQNAYRGLVRVM
                     PQAENARNHTQCDSLLLGKDCGAHTFPYVEVRNPTAKVEHEATTSKISADQLFYCRQR
                     GIS"
BASE COUNT          900 a         1337 c         1324 g          807 t
ORIGIN      
        1 cccgacgcac cgggttcgtc gccatcatct ccatggctgc aagctgatcc gtggctttca
       61 tcaggccgat ttccgaagcg agcgccgtac ccgcccgtcc cgcaaacaat aatgcggtaa
      121 tcacgggacc gagttccttc agcaaggaga acgcggtcag ggtcccgacc gaatcttcgg
      181 cattgaagcg agcaaggttc gagacgcctt gcaagccgag cacggcaccg acaaagaatc
      241 cgcagatcat gatgatgacc agcgacagcg cacccgcatt atgtatctgc tgcacgacca
      301 gcggaaagcg cttgaacagg acggtcggcg agtactgcag caggcgcagg aagaacagga
      361 acatcgcacc cgaagtgcgg aggaatcccc gaagaatgaa gtaaagctcg cggagcaacc
      421 ggggctactc ggcgtcacgg ccgagcagct gctcggcgta gtcgggtgcg gagtagtgaa
      481 acgcgacagg tccatcggcc ataccgtgca tgaactgccg taccagttcc gagctggtgt
      541 tgcgcagctc gtccgggctt ccggacgcga ccaccttgcc gtcggagatg agaaaactgc
      601 agtctgcgac cgttgatatt tcctgcacgt cgtggctgac gatgatgctc gagataccca
      661 gcgcgtcgtt catttttcgc accaggcgca cgatgacccc catcgaaatg gggtcaaggc
      721 cgacgaacgg ttcgtcatag atcaggatgt cggggtccat gaccatggcg cgcgcaagcg
      781 caacccggcg cgccatgcca cccgaaagct ctgccgggct catgtcggcc gcgccgcgca
      841 ggccgactgc gtgcagcttc gtcaggacga tatgacggat caaacgattg ggcagttttg
      901 tgtgctcacg cagcgggaat gcgacgttct cgaacacgtt gtagtccgtc agcagcgcgc
      961 cgttctggaa cagcatgccg atacgctgcc gcaagcggaa caggtcgcgc tggttcagtt
     1021 tggcgatatc gattccgtcg acttcgatat gcccgcgatc ggccaccagc tgccgggtga
     1081 tgagccgcag cagggtcgtc ttgcccgttc cgctggggcc catgatggcc gtgatcttgc
     1141 ctcgcgggat gttgatgttg agacccttga agatttcctt gggcccacgc gaataatcga
     1201 gatcgcgtat ctccacgatg ttctcggtcg ggttgctcat gcgtatgccc ttaccaatgc
     1261 cggacgcggc cggcaccgat cgaacggcag cgggccctgg aacggaaacc gcaggcgtcg
     1321 cgccttaagg ggccgactcc atctggctgt cgtacttgcc gaggtgcgcg aggctgttga
     1381 gtcgggcacg cttgtacagc gccttctgac cggcctgaaa ctgtgcttcc ttgccgcgcc
     1441 acgcatcgag cgcgggagcc tgcagcgccc tgccatagga aaacgagagc tcccagggca
     1501 gcggacccat ctggttcatc acgccaaggt gctccgtagc ctcctcggcg gactggcccc
     1561 ccgagaggaa tgcgatcccc ggcacctcag acggcacgtg cttcttcaga acgtcgaggg
     1621 tcgctcgcgc gacctgctct ggcccggcgc gattccgggc atcggtgccg gaaatgacca
     1681 tgttcggctt caggatgatg cccgagagct cgacccggtg atcctcaagc tcgccgaaca
     1741 cgagttccag cgctcgcgaa gtcacttctg cgcagcgatc aatgctgtgg tcgccgtcca
     1801 ttagcacttc gggctcaacg atcggcacga gccctgcctc ctggcaaagc gccgcgtaac
     1861 gcgcgagcgc atgcgcattg gcgcgcagac aaaagtctgt tggaatgcca tcgccgatgt
     1921 tgatcacggc gcgccacttg gagaacctcg ccccgagccc gacatactcg ttcagtcttt
     1981 cccgcagtcc gtccaggcct tcggtgatgg tttcgcccgg aaacccggcc aggggttttg
     2041 cacccttgtc gaccttgata ccggcgatca tgcccagatt attgagatac ctggcaaagg
     2101 gcacgccatc gctggtggac tggcggatgg tttcgtcgaa caggatgacc ccgccaatgt
     2161 aattcgcgac gccttcggcg gtgaacagca tttcacggta gcggcggcgg ttttcctcag
     2221 tcgattcggt gccgatggta tcgaagcgct tcttgatcgt cccgccactc tcgtccgccg
     2281 ccagtattcc cttgccgttc gccaccatgg cttttgcgac cgagttcagt tctttcagat
     2341 tcatgcagct ttctccggaa aaatcaggtg tgttgcggcg cgagtcggca gagtgctaca
     2401 ggcatacgca gcactggccg ggatcgggac tgcgtaggat agcaaacttt atgaggttgc
     2461 ctgtcctcga ggccgctccc cctatggcat accgaactaa tcaggactaa aatagtcctc
     2521 tttcgagcag cgggagatat ccggtgttgc gcatgagcaa attgacggat tacggcacag
     2581 tggtgctggc tgaactggcc agcggcaggg ccggctacgt cagcgcggcg gagcttgcag
     2641 aggccaccgg gctgggactg ccgacggtca gcaagctgct gaaaacgctg gcgaaggcag
     2701 gcctggtcat ctccacccgg ggctcccacg ggggctacag gctggcacgc gacgcgaacc
     2761 acatcagcgc cgccgaggtg atcgacgcgt tcgagggccc ggtatccatc accgagtgca
     2821 gcgccagcga aagccactgt ggactggaag ccatgtgcag cgtcggccat gcctggcagc
     2881 gcatcaacgt ggcgatacgc cgggcgctcg acgagatcag cctggccgac ctactccgcg
     2941 ccaacacccc gacgccgcgg ttcctgttcg ccggcacacc gatcaacgtg gagcgaaaga
     3001 gataactatg gcattcatcc cagacaaagt tgcggaaccg gtaggcggac ggaacgaaga
     3061 cgacatcgag tcgctcgtca accgccgtta caagcacggt ttcatcacag acatcgcgtc
     3121 cgacagcctg ccgccaggcc tggatgaaga cgtcatccgg gcgatatcgc tgcgtaagaa
     3181 ggaaccgccg ttcatgctgg aatggcgact gaaggcgtac cgtcactggc tgaccatgca
     3241 ggagccggag tgggcgcatg tgcattaccc gaagatcaaa tatcgggata tctcgtactt
     3301 ttccgccccg aagtccgacg aggaccggcc gaaaagtctc gacgaggtgg atccgaaact
     3361 cctggagacc tacgacaagc tgggcattcc gctgcatgag cgggcgagac tcgccggtgt
     3421 ggccgtggac gcagtcttcg acagtgtctc cgtcgccacg acgttcaagg aaaaacttgc
     3481 cgatgccggc gtcatcttct gttcgttttc agacgcggtg cagaaccacc cggagctggt
     3541 caggaagtac cttggaactg tcgtccccta tcgtgacaac ttcttcgcca ccctcaactc
     3601 ggcggtgttc agcgatggat ccttcgtcta cgtgccgaaa ggtgtgcgtt gcccgatgga
     3661 gttgtcaacg tatttccgca tcaacgcagc gaatacgggc cagttcgaac gcaccctgat
     3721 catcgccgac gagggcagcc acgtcagcta cctggaaggt tgcaccgccc cgatgcgaga
     3781 cgaaaaccag ttgcacgctg ctgtcgttga actggtcgca ctgaaggacg cggagataaa
     3841 gtactccacc gtgcagaact ggtatccggg cgacgaaaac ggccgcggcg ggatctacaa
     3901 cttcgtgacc aaacgcggtg actgtcgcgg cgacaattcg aagattgcgt ggacgcaggt
     3961 ggagaccggt tcggcgatca cctggaagta tccgagctgt atcctgcgcg gcgccaactc
     4021 cgtcggagag ttttattcag tggccgtggc gaacaacatg cagcaggccg ataccggcac
     4081 caagatgatt catatcgggc gcaatacgag cagtacgatc gtttccaagg gcatctccgc
     4141 aggaagggca cagaacgcat accgcgggct ggtgcgtgtc atgccgcaag cggagaacgc
     4201 gcgcaaccat acccagtgcg attcgctcct gctcggcaag gactgcggcg cccacacctt
     4261 cccgtatgtc gaagtgcgaa acccgactgc gaaggtcgaa cacgaggcga cgacatccaa
     4321 aatttccgcg gaccaactct tctactgccg ccagcgcggt atctcgga
//
LOCUS       DASUOM010000184         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1667233,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000184 DASUOM010000000
VERSION     DASUOM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667233"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFY27_03870"
     CDS             <1..387
                     /locus_tag="VFY27_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5764678.1"
                     /translation="VERALARGRKGDGATLIEAVTYRLSDHTTADDASRYRKKDEVEA
                     ARKLEPLIRMRKYLASLGLWDDEKEKQLLEQSAEKVDQAVQQYLNTPKAPVESMFDYM
                     YAELPEFLEEQREHAMHYGGDGEGHG"
     gene            347..1357
                     /locus_tag="VFY27_03875"
     CDS             347..1357
                     /locus_tag="VFY27_03875"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5764679.1"
                     /translation="MQCTTAATVKGMADVSLIDALTMAMAWELEHDPRVIILGEDVGV
                     NGGVFRATAGLQKRFGADRVQDTPLAEGMIAGVSIGMAVQGMRPVAEIQFMGFIYPTV
                     DQMISHAGRMRNRTRGRLSCPMVLRAPYGGGIHAPEHHSESTEAFFAHMPGIRVVIPS
                     SPARAYGLLLAAIRNADPVVFLEPKRIYRLNKQSVEDNGKELPLDVCYTLREGSDVTL
                     VTWGAMTYETLKAADQLAKSGVSCEVIDLASISPIDYETILESVEKTGRLVIIHEAAR
                     TAGVGAEIAAIVAEKGMYDLRGPIRRVTGYDVVMPLFRREMDYMPSVAKITDTIRESL
                     DD"
     gene            1391..2626
                     /locus_tag="VFY27_03880"
     CDS             1391..2626
                     /locus_tag="VFY27_03880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5764680.1"
                     /translation="MTTTFKLPDLGEGLPEAEIVAWHVAEGDSIKTDDLLVSVETAKA
                     VVEVPSPYTGTIARLHAKAGDVVETGAPLVDFDEESSGEPAKGQPEQEKAQPAASDGD
                     AQQRSDAGTVVGNVMSSDDVLQETAIAGGGRRRGGGRVKAAPAVRAEAKRRGIDLAEV
                     TPSGKNGQVTQADLEQFQQQSRQRARPSAKPKAQEQATAPGQSEPLRGPRRAMAQSMT
                     RSRDEVALCTLFDDADIHEWLNKRDFTPRILRSVVAGCAAEPALNGFYDAENVSRQLE
                     SRVDIAMAVDTGEGLIVPVIRGVERKSLEEIRTAVAQMKEATLNRTVAPRDMVNYTIT
                     VSNFGMLAGRYATPLMVPPTVAIVGTGKLQHDVVAVMGGIEVHRRIPLSLSFDHRCVT
                     GGEAARFLAAMIEDLEKPQ"
     gene            2684..>4555
                     /locus_tag="VFY27_03885"
     CDS             2684..>4555
                     /locus_tag="VFY27_03885"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX5764681.1"
                     /translation="MPRKQLRRSSDISERLIDEIIASAPRSSAIPEKKRIELFLRQYF
                     DNVPVEDMQGRSAAIMAQAAVSHLEFAAVRKPGQPLLRIFNPSSTKGGNQSSYTIIEM
                     VNDDMPFLVDSVSAAIDRQALTVHTTIHPVLRVKRDAKGELEEILRRSDTEGKFESYI
                     RFAVDREPDPQHLKVLEHEISKVLADVRVAVRDWRKMRQKMLDARATIDSGPPGADEE
                     LRRESKALLKWMADEHFTFLGYREYGLQPRGNKMYLQPVKGTGLGLLTREERGGRDVE
                     FTKELQRLTRRRDWLIITKANSRSTVHRRSYLDYVGVKIHDDEGNAIGEKRFIGLFTS
                     IAYSENPRNIPLLRLKVQRVLERSQLDPSGHRGKSLLHILDSYPRDELFQSSVRDLAR
                     TTNGILNLQERKRVKFFVRRDAFRRFFSCLVYIPREKYTTGVREKVERILLTAFEGTT
                     VDSSVQITDSPLARVHIIVRTSASDRPRISIRRIEEEIAESVVTWRDRLRVELAERFG
                     EDEGNSLYREYSEGFPPAYEGDVPPAIACLDVKRLDGLIKQEHDHFLFLYQPADAATD
                     QLNFRTFRKDKPLLLSHVMPVLEDLGTEVYSERPYRIKLKSGERFWIQDFELRFPNTA
                     "
BASE COUNT          968 a         1370 c         1449 g          768 t
ORIGIN      
        1 gtggagcgag ccctggctcg tggcaggaag ggggacggcg cgacgctgat cgaagccgtc
       61 acctacaggc tgagcgatca caccactgca gacgatgcca gccggtaccg caagaaagac
      121 gaagtggaag cagcccgcaa actggagccg ctcatccgca tgcgcaagta cctggcctcg
      181 ctcgggctgt gggacgacga aaaggaaaag caacttctcg aacagtctgc ggaaaaagtc
      241 gaccaggcgg tgcagcagta cctcaacacg cccaaggcac cggtcgagtc gatgttcgac
      301 tacatgtatg cagagctgcc ggagtttctc gaagaacagc gcgagcatgc aatgcactac
      361 ggcggcgacg gtgaagggca tggctgacgt ctcgcttatc gatgcgctga cgatggcgat
      421 ggcgtgggag ctcgagcatg atcccagggt catcatcctg ggtgaagatg tgggggtgaa
      481 tggcggcgtc tttcgtgcga cggccgggct gcagaagcgc ttcggtgcag accgggtcca
      541 ggacacgccg cttgccgaag gcatgatcgc cggcgtctcc atcggcatgg ccgtgcaggg
      601 catgcggccg gtggctgaaa tccagttcat gggcttcatc tatcccaccg tcgaccagat
      661 gatcagccat gccggcagga tgcgaaaccg cacccgcgga cgcctgagct gcccgatggt
      721 gctgcgcgct ccctatggcg gaggtatcca cgcgccggaa catcactcgg agagcacaga
      781 ggcgtttttc gcgcacatgc ccgggatccg cgtggtgatt ccttcatctc cggcacgtgc
      841 gtacggactg ctgctggcag cgatccgcaa cgccgacccg gtcgtttttc tcgagccaaa
      901 gaggatctat cggctgaaca agcagagcgt ggaggataac ggcaaagagc tgccgctcga
      961 cgtctgctac acgctgcgcg aaggcagtga tgtgacgctg gtgacctggg gcgcgatgac
     1021 ttacgaaacg ctgaaggcag ccgaccagct tgcgaagagc ggcgtaagtt gcgaggtgat
     1081 agacctcgcg tcgatcagcc cgatcgacta cgagacgatc ctcgaatcgg tggagaagac
     1141 cggccggctc gtcattatcc atgaagcggc cagaacagca ggcgtgggcg cagagattgc
     1201 tgcaattgtc gccgagaagg gaatgtacga tctgcggggt cccatacggc gtgtcaccgg
     1261 ttacgatgtc gtcatgccgc ttttccggcg cgagatggac tacatgccga gcgtcgcgaa
     1321 gatcaccgac actatcaggg aatccctgga tgactgatcc gcagcgccct ggaagtcgag
     1381 cgaggtacgc atgacgacga cattcaaact tccggatctc ggcgaaggcc tgcccgaggc
     1441 cgagatcgtt gcctggcatg tcgcggaagg cgattccata aagaccgacg acctcctggt
     1501 gtcggtagag accgctaagg ccgtggtgga agtgccctcg ccttacacgg ggaccatcgc
     1561 ccggttgcat gccaaggcag gcgatgtggt cgagaccggc gcgcccctgg tcgatttcga
     1621 cgaggagtcc tccggcgaac ccgcgaaagg acagccggag caggaaaagg cgcaaccggc
     1681 agcaagtgac ggcgatgcgc agcaaaggag cgatgccggc accgtcgtcg ggaacgtgat
     1741 gagcagcgat gacgtccttc aggagactgc cattgccggt ggcggcaggc ggcgcggcgg
     1801 gggccgcgtg aaagcagcgc ccgcggtccg tgccgaggca aagcgccggg gcatcgacct
     1861 ggcagaagtc accccctcgg gcaagaacgg ccaggttaca caagcggacc tcgagcagtt
     1921 ccagcagcag tcccggcaac gggcgagacc ttccgccaag ccgaaggcgc aggagcaggc
     1981 aacagcgccc gggcagagcg agccattgcg cgggccgcgc cgcgcgatgg cgcaaagcat
     2041 gacccgctcc cgcgacgaag ttgcactctg cacgctcttc gacgatgcag acatccatga
     2101 gtggctcaac aagcgcgact ttaccccacg cattctcagg tctgtcgtgg cgggctgcgc
     2161 ggcggaaccg gcgctgaatg gtttttacga cgccgaaaac gtcagccggc aactcgagtc
     2221 gcgggtcgat atcgcgatgg cggtcgatac cggggagggc ctgatcgtgc cggtcatccg
     2281 cggagtcgaa cgaaagtcgc tcgaggaaat ccgcacggcc gtcgcgcaaa tgaaggaagc
     2341 aacgctgaat cgcaccgttg caccccggga catggtgaac tacacgataa cagtgtccaa
     2401 cttcggcatg cttgcgggcc ggtatgcaac gccgctcatg gtaccgccga ccgtcgcgat
     2461 cgtcggcacc ggaaaactgc agcacgacgt ggtcgcggtt atgggcggta tcgaggtgca
     2521 ccggcgaatc ccgctgtctt taagcttcga tcatcgttgc gtcacgggag gtgaagcagc
     2581 acgtttcctt gctgcaatga tcgaggactt ggaaaagcct cagtaaagcg gtatagagtc
     2641 ccgcacattc cacaatgcac cgcccgcccg gggtcctctt caagtgccaa gaaagcaact
     2701 tcgtcgctcg agcgacatct ccgaacgcct gattgacgag atcatcgcgt cggcgcccag
     2761 gtccagcgcg atacccgaga aaaagcgcat cgagctgttt ctgcgccagt acttcgataa
     2821 cgtgcccgtc gaagacatgc agggacgctc cgcggcgatc atggcgcagg cggccgtttc
     2881 acatcttgaa tttgctgccg tgcggaagcc cggacagccg ctcttgcgga tcttcaaccc
     2941 gtcctcgacc aaggggggca accagtcctc ctacaccatc atcgagatgg tcaacgacga
     3001 tatgccgttc ctggtcgact cggtatccgc ggccatcgat cggcaggcgc tcacagtaca
     3061 cacgaccatc cacccggtgc tccgggtgaa gcgggacgcg aaaggcgagc tcgaggagat
     3121 tctcaggcgc tcggacaccg agggcaagtt cgaatcctac atccgcttcg ctgtcgaccg
     3181 ggaaccggac ccgcaacatc ttaaggttct cgagcatgaa atttccaagg tgcttgcgga
     3241 cgttcgggtt gcggtccgcg attggcgcaa gatgcgccag aaaatgctgg atgccagggc
     3301 gacgatcgac agcggcccgc cgggtgccga tgaggagctg cgtcgcgaaa gcaaggcctt
     3361 gctaaagtgg atggcggacg aacactttac cttcctcggg tatcgcgaat atggcttgca
     3421 gccgagaggc aacaagatgt atctccagcc ggtcaagggg accgggctgg gcctgctcac
     3481 gcgcgaggaa cgcggcggca gggacgtcga attcaccaag gaattgcagc gcctgacacg
     3541 ccgtcgcgac tggctgatca ttacgaaagc aaattcccgc tcgacggtgc accggcgcag
     3601 ctatctcgac tacgtcggcg tcaagatcca cgacgacgaa ggcaacgcga tcggtgaaaa
     3661 gcggtttatc ggcttgttca cctcgatcgc ctacagcgag aacccgcgga acatcccgtt
     3721 gctgcgcctg aaagtgcaac gggtgctgga gcgctcacag ctcgatccat ccggacaccg
     3781 cggcaagtcc ctgctgcaca tcctggactc gtatccgcgc gacgagcttt tccagagttc
     3841 ggtgcgggac ctggcgcgca cgaccaacgg catcctcaat ctccaggaac gcaagcgggt
     3901 aaaatttttc gtccgcaggg acgcgttccg ccggttcttc tcatgcctgg tttacatccc
     3961 gcgggaaaaa tacaccacgg gcgtgcggga aaaagttgag cgaatcttgc tgaccgcctt
     4021 cgaggggacg acggttgact ccagcgtgca gatcacggat tcgccgctcg cccgcgtgca
     4081 catcatcgtc aggaccagtg cgagcgatcg tcctcgcatc agcatccgcc gcatcgagga
     4141 ggagatcgcg gaatcggtcg tcacctggcg cgacaggctt cgcgtggaac tcgcggaacg
     4201 cttcggcgag gatgaaggta actcgctcta tcgcgagtat tccgaaggct ttccgccggc
     4261 gtacgagggt gatgtacccc cggccattgc ttgcctcgac gtgaagcggc tggatggcct
     4321 gatcaagcag gagcacgacc acttcctgtt cctgtaccag ccggcggacg cggcgacgga
     4381 ccagctcaac ttccggacgt tccgcaagga taaacccttg ttgctgtcgc acgtgatgcc
     4441 ggtacttgag gacctgggaa ccgaggtcta cagcgaacgt ccctaccgca tcaaactgaa
     4501 aagcggcgag cggttctgga tccaggattt cgagctccgc ttcccgaaca cggcc
//
LOCUS       DASUOM010000185         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_309265,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000185 DASUOM010000000
VERSION     DASUOM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309265"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFY27_03890"
     CDS             <1..216
                     /locus_tag="VFY27_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5764682.1"
                     /translation="GNDHAIASAVRNRLRDDSVLGQYDLRIETVNRRVILHGTVDSYE
                     ARERAIRLAGAVDGVERVDSRLRIGGT"
     gene            313..627
                     /gene="grxD"
                     /locus_tag="VFY27_03895"
     CDS             313..627
                     /gene="grxD"
                     /locus_tag="VFY27_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5764683.1"
                     /translation="MDVKERIEQQLKENKVLLYMKGTPDFPQCGFSGQTVMALKSIGQ
                     PFAYVNIFEDPEIREGLKTYSNWPTFPQLYVNGELVGGCDIVVEMYKSGELQQLLSEV
                     AA"
     gene            complement(724..1404)
                     /gene="rnt"
                     /locus_tag="VFY27_03900"
     CDS             complement(724..1404)
                     /gene="rnt"
                     /locus_tag="VFY27_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX5764684.1"
                     /translation="MKPFAICERFRGFLPVVVDVETGGFNSRTDALLEIAAVFVDVHD
                     DGTFTRGKTTRYHVKPFDGANMEPASLAVIGIDPNHPLRPAIDERDALQRVFRDVRTA
                     IRAHGCSRAILVGHNSSFDLAFLNEAIERTGIKRNPFHPFSSFDTATLCGVAFGQTVL
                     SRAVHAAGLKWDESQAHSAAYDAEITADVFCEIVNRFRSVFESARQQDFQVMEDVMQH
                     GETFEESR"
     gene            complement(1470..3020)
                     /locus_tag="VFY27_03905"
     CDS             complement(1470..3020)
                     /locus_tag="VFY27_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX5764685.1"
                     /translation="MADVSLASMYAKGQEKSRIKITQVTAAPTAAAIASIDQLLVVVP
                     ERVPASVWRIIPQGHKVQALMRRRAQGHAQGNAPSVRTRINNKRQSLLLVGTVAPEGE
                     AFDLLSFARKMVAAAACEPGGNLGIWAAGFSEADAARITAHLTTAALASGFNLADFKT
                     KPRPQRIRSIRILGRRERLDLRRVEAEARGNNLARWLTAMPPNKLDAAEYILFLRELA
                     KEYGWRFKKYGVRELESMGAGAFLAVAQGNQDDSAGIVRLQYRPGASTGTPAVSLVGK
                     GIIFDTGGTNLKPFLSMADMHIDMGGSAVAVGALLAMTELEYPHAVDCWMPITENRIG
                     PNAYKSQDVITAANGKTIQVVHTDAEGRLALADALVFACREKPRLIIDYATLTGACIN
                     AITRSYSGVFTNRPDWHPLLKKSGRDSGERVWPFPQGKEFLGELESEVADIKQCTPDA
                     GGDHILAATFLNEFVDRDVPWIHVDLSASKRKGGLGAIATDITGFGVRLTMDLVLDKQ
                     LPDSPGIS"
     gene            3218..5902
                     /gene="aceE"
                     /locus_tag="VFY27_03910"
     CDS             3218..5902
                     /gene="aceE"
                     /locus_tag="VFY27_03910"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5764686.1"
                     /translation="MTDLSKFWSRFDDIDPTETREWLESIDSVLRQHGPERAHFLLNQ
                     VIDFARRSGAYLPYSPNTAYLNTIATGRQPEYPGDRSIEQRIESYIRWNAMAMVVQAN
                     RKSSEYGGHIASYASSATLYEVGFNHFWRAPTDGHGGDMVFLQGHSSPGFYARAYLEG
                     RLDEQQLSQFRKEVGGGLSSYPHPWLMPEFWQYPTVSMGLGPMMAIYQARFMRYLENR
                     EMIPASDRKVWCFLGDGEMDEPESMGAITMPVREGLDNLVFVVNCNLQRLDGPVRGNG
                     KIIQELEAAFGGAGWNVIKVIWGSRWDPLLARDHTGLLRRVMEECVDGEYQNFKSKDG
                     AYVRENFFGRYPETKAMVADMSDDEIWRLNRGGHDPLKVHAAYHSAVRHKGQPTIILA
                     KTIKGYGMGTAGEGQNIAHQAKKVGVEALKTFRDRFNIPLSDKEIDEDVPFCKFKPDS
                     AELEYLHERRKALGGYLPQRRTKPLPTQKLKAPPLDLFKSALEGTGEREASTTMVFVR
                     MLTALLRDKDVGKYVVPIVPDEARTFGMEGMFRQVGIYSSKGQLYTPQDAEQLMFYKE
                     DKKGQILEEGINEAGSFCSWLASGTSYSNHGIQTVPFYIYYSMFGFQRIGDFVWAGAD
                     QRSRGFMVGGTAGRTTLAGEGLQHQDGHSHVLASTIPNCVSYDPAYGYEIAVIVQDGM
                     RRMIDEQESVFFYFTCMNENYVHPPLPEGVEKGILKGMYLLQSGGGGDVRVQLMGSGT
                     ILREALAAADLLQQDFDIPSDVWSVTSFNELRRDALKAERWNQLHPEEEPRSSYLEEC
                     LAGRPGPFVAATDYMKIVPDQLQRWMPGRYVSLGTDGFGRSDARKALRNFFEVDRYYI
                     AVVALKALADEGKCDKARVKEAIARYGIDPDKPDPVTL"
BASE COUNT         1246 a         1760 c         1890 g         1220 t
ORIGIN      
        1 gggaacgatc acgctatcgc ttcggcggta cgcaaccggc tcagggatga ttcggttctg
       61 ggccagtacg acctgcgcat cgaaacggta aatcggcggg tgatcttgca tggaacggtc
      121 gatagttacg aggcacgcga acgcgctata cgactggcgg gtgccgtgga cggcgtggag
      181 cgtgtcgaca gccgcttgag aatcggagga acttgactcg agtggcgagg gtcgtaaagc
      241 attaagtgga gtacgctttt ttcagttccc ggatttgcgg gacgaactaa tcaattcggc
      301 aggtaagaca acgtggacgt aaaggaaaga attgagcagc agttgaagga aaacaaggtg
      361 ttgctttaca tgaaaggcac acctgatttt ccgcaatgcg gattttcggg ccagacggta
      421 atggctctca agtccatagg ccagcctttt gcctatgtga acatattcga ggatccggaa
      481 atccgcgaag gtttgaaaac ctactccaac tggccgacgt ttccccagct ctacgtcaat
      541 ggcgaactgg ttggcggttg cgacatcgtg gtggagatgt ataagtccgg ggagctgcag
      601 cagctgctgt cggaagttgc tgcctaggaa ctcgtaggag ttcgccgagc gagcgagtcc
      661 caagccgtat cgcaagccgc cagcgtggcg ggctcctaca tcggctgccg ttgcaggttt
      721 cttctagcgg ctctcctcga aagtttcccc gtgctgcatc acgtcttcca tgacctggaa
      781 atcctgttgc cgtgcagatt cgaacacgct gcggaagcgg ttgacgatct cgcagaagac
      841 gtccgccgtg atctccgcgt cgtaggcagc ggagtgggcc tggctttcgt cccatttcag
      901 tcccgccgca tgcaccgccc gtgacaggac agtttgcccg aaggcgacgc cacacaatgt
      961 agcggtatcg aagctgctga agggatggaa gggatttcgt ttgatgccgg ttctttcgat
     1021 cgcctcgttc aggaaggcaa ggtcgaacga cgagttgtgg ccaaccagga ttgcccgcga
     1081 acatccgtgc gcgcgtatgg cagttctcac atcgcgaaat acccgctgca gcgcgtcgcg
     1141 ctcatctatt gccggacgta acgggtggtt cggatcgatg ccgatcacgg caagcgacgc
     1201 gggttccatg tttgcgccgt cgaacggctt tacgtgatag cgggtggttt tgccgcgggt
     1261 gaacgttcca tcgtcatgca catccacgaa aaccgctgca atttccagca gggcatccgt
     1321 ccgtgagttg aaaccgccgg tttcgacatc caccacgacc ggaagaaaac cgcgaaagcg
     1381 ctcacagatc gcaaagggtt tcatcgacgg aaccatatca gatcgaaaag cgaaatgtcg
     1441 cgcggccaac gagaatgttg cgcggggagt caggagatgc cgggagaatc cggcagctgc
     1501 ttgtccagga ccaggtccat tgtcaggcga actccgaagc cggtaatgtc cgtcgcgatt
     1561 gctccaagtc cgcccttgcg ttttgacgcg ctgaggtcaa cgtggatcca cggaacgtct
     1621 ctgtcgacga actcgttcag gaacgtcgcg gcgaggatgt ggtcgcctcc tgcatccggc
     1681 gtgcattgct tgatgtcggc aacttcgctc tccagttcgc ccaggaactc tttcccctgc
     1741 ggaaagggcc agacgcgctc gccactgtcg cggcccgact tcttcagcag tggatgccag
     1801 tcgggacggt tcgtgaaaac gccgctgtaa ctgcgggtaa tggcgttgat gcaggcgccg
     1861 gtcagcgtgg catagtcgat gatgagccgg ggcttttccc tgcatgcaaa cacgagagca
     1921 tccgcaagcg cgagccgccc ctcggcatct gtgtgcacga cctggatggt cttgccgttg
     1981 gccgcggtga tgacgtcctg ggacttgtaa gcattgggtc cgatccggtt ctccgtaatc
     2041 ggcatccagc agtcgacggc atgcgggtac tcgagttccg tcatcgcgag cagcgctcca
     2101 acggcaactg cgctgccgcc catgtcaatg tgcatgtccg ccatcgaaag aaacggcttc
     2161 aggttcgttc cgccggtatc aaaaatgatg cccttgccaa cgaggctaac cgcgggcgtg
     2221 ccggtagagg cgccagggcg gtactgcagc ctgacgatac cggcgctgtc gtcctggttg
     2281 ccttgcgcta cggccaggaa cgcccctgca cccatcgact cgagttcgcg cacaccgtac
     2341 ttcttgaagc gccagccgta ctctttcgct agctcgcgca ggaacaggat gtattccgcc
     2401 gcgtccagct tgttcggcgg catcgccgtc agccagcgcg caagattatt accgcgcgcc
     2461 tcggcctcga cgcgccgaag gtccaggcgc tcgcgacgtc cgagtatgcg aatgctgcgg
     2521 attcgctgcg gtctcggctt cgtcttgaaa tctgcgagat tgaagcctga tgcaagcgcg
     2581 gccgttgtaa ggtgcgccgt gatgcgcgcg gcatcggctt cactgaagcc ggctgcccag
     2641 atgcccaggt tcccgccggg ctcgcacgcc gcggcggcga ccatctttcg cgcgaacgaa
     2701 aggagatcga atgcttcccc ttcaggggcg acggtgccga ccaggagcag cgattgccgc
     2761 ttgttgttga tgcgggtcct gaccgacggc gcgttcccct gggcatgtcc ctgggcacgc
     2821 cgcctcatca gtgcctggac cttgtgaccc tgcgggatga ttctccagac tgaagccggc
     2881 actctctcgg gcacgaccac cagcaactga tcgatgctcg ctattgcggc ggccgtggga
     2941 gctgccgtca cctgcgtgat ttttatgcgg cttttttcct gtccctttgc gtacatgctt
     3001 gcgagggaga catctgccat ttctgcttcc atatcaacac taacctttcc cggcctgttg
     3061 ggccataata cgcgactcaa acgtacgtcg ccggtgatga acgaatcccg gttcgccgtt
     3121 tcagcaatac cccacccgcg gtgcagtgca tcattcattg aaaccggtgc atggacaatt
     3181 gcagggataa ctttattcat gattctatgg gcgctttatg accgaccttt ctaaattctg
     3241 gagcagattt gacgacattg atccgaccga gacccgcgaa tggcttgaat cgatcgattc
     3301 agtgttgcgg cagcatgggc cggagcgcgc gcacttcctg ctgaaccagg tcatcgattt
     3361 cgcccgacgc tccggcgctt atcttcccta tagtccgaac acggcgtacc taaatacgat
     3421 tgcgacgggc cggcagccgg aatatccggg cgaccgctcc atcgagcagc gcatcgagtc
     3481 ctacattcgc tggaatgcca tggccatggt cgtgcaggca aaccgcaaga gttcggaata
     3541 cggcggtcac atcgcgagct acgcgtcttc agcaacgctg tacgaagtcg ggttcaatca
     3601 cttctggcgc gcgccgaccg acgggcatgg cggcgatatg gtcttcctgc agggacactc
     3661 atctcccggc ttctatgcgc gcgcttacct tgaggggcgc ctcgacgagc agcaactgag
     3721 ccaattccgc aaagaagtcg gcggcgggct ttcgtcgtac ccgcacccgt ggctgatgcc
     3781 ggaattctgg cagtacccga ccgtctccat gggactgggt ccgatgatgg cgatttacca
     3841 ggcgcgcttc atgcgttacc tggaaaaccg ggagatgatc ccggcgagcg atcgcaaggt
     3901 gtggtgtttt ctcggcgacg gcgaaatgga cgagccggaa tcgatgggcg ctatcactat
     3961 gccggtgcgt gaagggctgg acaacctcgt cttcgtcgtc aactgcaacc tgcagcggct
     4021 ggacgggccg gtgcgcggca atggcaagat catccaggaa ctggaggcgg cattcggcgg
     4081 cgcgggctgg aacgtcatca aggtcatctg gggctcgcgc tgggatccgc tgctcgcgcg
     4141 ggaccacaca ggcctgcttc ggcgtgtcat ggaagaatgc gtcgatggtg aataccagaa
     4201 cttcaagtcc aaggacggcg cttacgtgcg cgagaatttc ttcggtcgct acccggagac
     4261 gaaggcaatg gtcgcggaca tgtccgacga cgaaatctgg cgcctgaacc gcggcggaca
     4321 cgatccgctg aaggtgcatg ctgcctacca cagcgccgtc cgtcacaagg gccagccgac
     4381 aatcatcctg gcgaaaacca tcaaggggta cggcatgggt accgccggcg aagggcagaa
     4441 catcgcccac caggcgaaga aggtcggcgt cgaagcgctc aagactttcc gggaccgttt
     4501 taatatcccg ctgtcggaca aggaaatcga cgaggacgtg ccgttctgca agttcaagcc
     4561 ggacagcgcg gagctcgaat acctgcacga aagacgcaag gcgctgggcg gctacctgcc
     4621 gcagcgccgc acgaagcccc tgccgacgca gaagctgaaa gcgccgccgc tggatctgtt
     4681 caagtcggcg ctggagggga cgggcgagcg tgaagcctcc accaccatgg tgttcgtgcg
     4741 catgctcacg gcgctgctgc gcgacaagga tgtcggaaaa tatgtcgtgc cgatcgtccc
     4801 cgacgaagcg cgcaccttcg gcatggaagg catgttccgc caggtcggca tttattcgtc
     4861 gaaaggccag ttgtacacgc cgcaggatgc cgaacagctg atgttctaca aggaagacaa
     4921 gaagggccag atcctcgaag agggcattaa cgaagccggc tcgttctgct cctggctggc
     4981 gtcgggtacg tcctacagca atcacggcat ccagacggtc ccgttctata tctactattc
     5041 catgttcggc ttccagcgca tcggggattt cgtctgggcc ggtgcggacc agcgctcgcg
     5101 cggcttcatg gtcggcggaa cggcagggcg cacgacgctc gccggcgaag ggctgcagca
     5161 ccaggacgga cacagccatg tgctcgcgtc taccataccg aattgcgtgt cgtatgaccc
     5221 tgcctatggc tatgaaatag ccgtgatcgt gcaggacggc atgcgccgca tgatcgacga
     5281 gcaggagagc gtgttcttct acttcacctg catgaacgag aactacgtgc atcctccctt
     5341 gcccgaaggg gtagagaagg gaattctcaa agggatgtac ctcctgcaga gcggaggagg
     5401 gggcgatgtg cgcgtgcagc ttatgggctc cgggaccatt ttgcgagagg cgctagccgc
     5461 agccgacctt ctgcaacagg atttcgatat cccttcggat gtatggtccg tcaccagctt
     5521 caacgagctg cgccgcgatg cgctcaaggc ggaacggtgg aaccagctgc accccgaaga
     5581 agagccgcgc tcgagctacc tggaagagtg cctcgccgga aggccaggcc cattcgttgc
     5641 ggccacggat tacatgaaga tcgtgccgga ccagttacag cgatggatgc cgggtcggta
     5701 tgtgtcgctc ggtacggacg gcttcggccg cagcgacgcc cgcaaggcgc tgcgcaattt
     5761 cttcgaagta gaccggtatt acattgcggt ggtcgcgctg aaggcgctgg ccgacgaggg
     5821 caagtgcgac aaggcaagag tgaaagaggc gatcgccagg tacggcatcg atcccgacaa
     5881 gccggacccg gtgactctct gatacgccgg taatgattgc gtgtggtcta tataatacct
     5941 tatatattag tagcctgtac cgctaatctc gaaaaaatcg agcatggggc gaaaggcatg
     6001 acgcgctgag gttgaatggc aggtggcgaa aagcatcgac ataaaggttc cggatctcgg
     6061 ggatttctca gacgttgaga tcatcgaagt gctggtgaag gcgggcgacc gcgtct
//
LOCUS       DASUOM010000186         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1500046,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000186 DASUOM010000000
VERSION     DASUOM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500046"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1141)
                     /locus_tag="VFY27_03915"
     CDS             complement(242..1141)
                     /locus_tag="VFY27_03915"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5764687.1"
                     /translation="MNVHSLQGTADWRDYYELTKPRVVLLIVFTAIVGMFLSVPGWPG
                     AGALLFGTLGIGLAASSAAAINHLLDARIDIQMSRTRRRPLPQGRLSEAHAVAFAAIL
                     CALAMLILWFLINPLTAILTFASLIGYAVVYTVWLKRATPQNIVIGGAAGAAPPVLGW
                     AAVTGEVHANALLLFLIIFVWTPPHFWALAIARKADYEKAGIPMLPVTHGDKFTRQFI
                     LLYTILLVLVTVLPYLSGMSGLIYLAAALALGGIFLYYAIRMRLDESPRLPMRVFRYS
                     INYLMLLFAALLLDHYFMVTFGL"
     gene            complement(1188..1766)
                     /locus_tag="VFY27_03920"
     CDS             complement(1188..1766)
                     /locus_tag="VFY27_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764688.1"
                     /translation="MTNNAGKHLPAWLQLSLLAILFGGPLILAAWLYYGGSLGPSGRT
                     NHGALLEPIVNLPERVPNSAVTDLSDDRWLLVYATENECGAKCRDALYVLRQSRLMLG
                     NDMQRLLRVFLRGPVAPDTVFIEEQYPGLAIIEEPQLIELLEARRPREMSKGGFFLVD
                     PLGNLVMYFPPEIDPGDMVDDIEHLLDLSRIG"
     gene            complement(1763..2503)
                     /locus_tag="VFY27_03925"
     CDS             complement(1763..2503)
                     /locus_tag="VFY27_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX5764689.1"
                     /translation="MTLGNLAPRDLLPPLAGIALVVLFVNLGVWQLERAEEKRAIEAA
                     FSDSGEGAVVNDIAGERGGKVGDGEVRLYQPLSASGRYLADRQFLIDNMILEGRVGYF
                     VVTPFEWSRDEPLLLVNRGWLQKSAAAMPSAIGMDASAAETITGRAGRLPRVGVRAGD
                     VFTGGPEWPRIANWPTLDELAAALGRDVLPFILLADPEPGSNLVRHWEPRQAGPMRHI
                     GYAVQWFALALAVIATAFVVYRKKREER"
     gene            complement(2543..3409)
                     /locus_tag="VFY27_03930"
     CDS             complement(2543..3409)
                     /locus_tag="VFY27_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5764690.1"
                     /translation="MSHAEDRYYVPHSSHWPIVGSIGLFVLMVGVSNWLNGAQAGFWI
                     MWAGIAVIVFMFAGWFSNVIAENQAGKFNKQVDKSFRMGMFWFIFSEVMFFSCFFGAL
                     FYARNMAIPWLGGASNNFFTNLFLWNAYESTWPTAGPEQLGGEFEAMEPFGLPLINTA
                     ILLTSSITVTIAHHAIQHNRQVMLRVFLALTFLLGYLFVYLQAVEYVHAYQEMNLKLT
                     TGIYGSTFYMLTGFHGLHVTLGAIMLTVIWLRAMKGHFTPGNHFAFEAVAWYWHFVDV
                     VWLGLYIFVYWM"
     gene            complement(3430..3993)
                     /locus_tag="VFY27_03935"
     CDS             complement(3430..3993)
                     /locus_tag="VFY27_03935"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5764691.1"
                     /translation="MNQDSGALALRLLLCALGMFAFGYALVPLYDVFCEVTGFGGRTN
                     ATPAVVAEEVDDSRSIDLEFVTTVNEYAPWTFRSSVPGMTVHPGGMYEATFVATNLTD
                     RPMTAQAVPSVAPQQASRFFKKLDCFCFTTQEFAANETRELPVRFIIDSDLPAHVDTI
                     TLSYTFFDTARMSRNDASGAHSTSTAN"
     gene            complement(4137..>4824)
                     /locus_tag="VFY27_03940"
     CDS             complement(4137..>4824)
                     /locus_tag="VFY27_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5764692.1"
                     /translation="VGMPLAGQLFFMLSTMLIAIPTGVKVFNWTATMWKSSMTFETPM
                     LFALSFLFLFTIGGFSGLMLAIVPADFQYHDTYFVVAHFHYVLVPGALFAILGATYYW
                     LPKWTGRMYNEKLGQIHFWLTAIFVNVTFFPMHFLGLAGMPRRIPDYATQFTDWNFVA
                     SIGAFGFGLSQLIFVYVVISCMRGRGEPATAEVWDNPKGLEWTVPSPAPFHTFEVPPK
                     VSAEPAPGFS"
BASE COUNT         1026 a         1444 c         1507 g          847 t
ORIGIN      
        1 gatgagggtg gcactgctca tgaacgcatt catcgggggt ggttgaggag tcgcaagcga
       61 cagggggagg gcttgtcagg aacgccgtga acccatccat gggggctctg cgcaaacatc
      121 cctgtttgcg acagtcctga caagccctcc ccccagccaa gacttcgcgc tggacgtggt
      181 ggcgttcgcg gataatgctc gtgccagctg acagcgaccc tgcggccgtg acggccgcag
      241 gtcaaagccc gaaagtcacc atgaagtagt gatccagcag cagcgctgcg aacagcagca
      301 tgagatagtt gatggagtat ctgaaaaccc gcatcggcag gcgcggactt tcgtcgagcc
      361 tcatgcggat cgcgtaataa agaaaaatgc cgccgagcgc cagggcagcg gcgaggtata
      421 tgaggccgct catgccggag agatacggca gcacggttac cagcaccagc aagatcgtgt
      481 agagcaggat gaactggcgc gtgaacttgt ccccatgcgt taccggcagc atggggatgc
      541 ctgctttttc gtagtcggcc ttgcgcgcga tggcgagcgc ccagaaatgc ggcggggtcc
      601 agacgaagat gataaggaac aggagcagtg cattggcatg tacttcgccg gtgacggcgg
      661 cccagccgag caccggcggg gcagcgccgg cagcgccgcc gatgacgata ttctggggcg
      721 ttgcgcgctt cagccagacg gtgtagacga cggcatagcc gatcagcgac gcgaaggtca
      781 ggatcgccgt cagcggattg atgagaaacc acaggatgag catcgcgagc gcgcagagga
      841 ttgccgcgaa tgcgactgca tgtgcttccg agagcctgcc ctgcggcagc gggcgcctgc
      901 gggtgcggga catctggatg tcgatgcgcg cgtcgaggag gtgattgatt gccgcggcgg
      961 atgacgccgc cagccctatg ccgagcgtgc cgaacagcag tgcgccggct cccggccatc
     1021 ccggaacaga aaggaacatc ccgacgatag ccgtgaacac gatcagcagg acgacgcgcg
     1081 gcttcgtcag ctcgtagtaa tcgcgccagt cggcggtgcc ctgcaggcta tgtacgttca
     1141 agactcatgc tccggttgtt ccgaggctgc cgggcttccc ggcaaggcta gccgattcgc
     1201 gacagatcga gcaggtgctc gatatcgtcg accatatcgc ctggatcgat ttcgggaggg
     1261 aaatacatca ccaggttccc tagcgggtcc acgagaaaaa agcctccctt ggacatctcc
     1321 cgcggccggc gcgcctccag gagctcgatc aattgcggct cctcgatgat cgcgagcccc
     1381 ggatactgtt cctctatgaa cactgtatcc ggcgctacgg gaccgcgcaa gaataccctc
     1441 agcaggcgct gcatatcgtt gccgagcatc aaccgggatt gccgcaatac gtacagggca
     1501 tcgcggcact tcgcgccgca ttcattctcg gtggcgtata ccagcagcca gcgatcgtcg
     1561 gacagatctg taacggccga gttcggtacc ctctcaggca ggttgactat cggctcgaga
     1621 agcgcaccgt gatttgtccg gcccgagggg cccagggagc caccgtagta gagccacgcc
     1681 gccaggatga gcggaccgcc gaacagtatc gcgaggagag aaagctgcaa ccaggccggc
     1741 aggtgcttcc cggcattgtt cgtcatcgtt cctcccgctt cttccggtac accacgaatg
     1801 ccgtcgcaat gaccgccagt gcgagtgcga accactgcac cgcatagccg atatggcgca
     1861 tgggtcccgc ctggcgcggc tcccagtggc gtacgaggtt gctaccgggc tccgggtcgg
     1921 ccagcaggat gaatggcagc acatcccggc cgagcgccgc cgccagttcg tcgagcgtgg
     1981 gccagttcgc aatgcgcggc cattccggac ctccggtgaa cacatcgccg gcccttacgc
     2041 cgacccgcgg aagccttccc gctctaccgg taatggtttc cgcggcgctg gcgtccatcc
     2101 cgatcgcgga tggcatggcc gctgccgact tctgcaacca gccccgattc acgagaagca
     2161 aaggctcgtc cctcgaccat tcaaacggcg tcacgacgaa atagccgacg cgaccttcga
     2221 gaatcatgtt gtcgatgagg aactggcggt cggcgaggta tcgccccgag gcggacagcg
     2281 gctggtacaa ccgaacctcg ccatcgccga cttttccacc gcgttccccg gcgatgtcgt
     2341 tgaccaccgc gccctcgccg gaatccgaga aagccgcctc aatggctctt ttttcctccg
     2401 cccgctccag ctgccagaca cccaggttca cgaacagcac taccagcgct attccggcca
     2461 gcggtggcag gagatcccgt ggagccaggt ttcccagggt cacgtgacta gcaggacacg
     2521 ggtgaggcgc ccgaccgctg gtctacatcc agtagacgaa tatgtagagt cccagccaca
     2581 cgacgtcgac gaagtgccag taccacgcca cggcttcgaa tgcgaagtgg ttcccgggcg
     2641 taaagtgccc cttcatcgcg cgcagccaga tgacggtcag cattatcgcg cccagggtca
     2701 cgtgcaggcc gtggaatccg gtcagcatgt agaaggtcga gccgtaaatg ccggtcgtca
     2761 gcttgaggtt catctcctgg taggcatgta cgtactccac cgcctgcagg tagacgaaca
     2821 ggtaaccaag gaggaaggtc agcgcgagga aaaccctgag catgacctgc cgattatgct
     2881 gaatggcgtg gtgggcgatc gtgaccgtga tgctgctggt gagcaggatt gccgtgttga
     2941 tcagcggcag gccgaacggc tccatcgctt cgaattcgcc accgagttgc tccggaccgg
     3001 cggtcggcca ggtgctttca taggcgttcc acaggaacag gttcgtgaag aaattattgc
     3061 tggcaccgcc gagccaggga atagccatgt tgcgcgcgta gaagagcgca ccgaagaagc
     3121 aggagaagaa catgacttcc gagaagatga accagaacat ccccatgcgg aatgacttgt
     3181 cgacctgctt gttgaacttg cctgcctggt tctcggcaat gacgtttgaa aaccagccgg
     3241 caaacatgaa cacgatgaca gcgattcccg cccacatgat ccagaaaccg gcctgggcac
     3301 cgttcagcca gttggacacg cctaccatca gcacgaacag gccgattgaa ccgacgatcg
     3361 gccagtgact gctgtgggga acgtagtatc gatcttcagc gtgagacatt tggattaccg
     3421 tggatcaggc taattggcgg tggacgtcga atgcgcgccc gatgcgtcgt tgcgcgacat
     3481 gcgggccgta tcgaagaatg tgtaagacag cgtgatcgta tcgacatgcg ccggcaggtc
     3541 ggagtcgatg atgaagcgca caggcagttc acgcgtctcg ttcgcagcaa actcctgcgt
     3601 cgtgaagcag aagcaatcca gcttcttgaa aaaccggctg gcctgctgcg gcgcaacgct
     3661 cggcacggcc tgcgccgtca tcgggcggtc cgtaaggttg gtcgcgacga aagtggcctc
     3721 gtacatgccg ccgggatgaa cggtcatgcc gggtaccgac gagcggaacg tccatggcgc
     3781 gtactcgttg acggtcgtta caaactcgag gtctatgctg cggctgtcgt cgacttcttc
     3841 cgcaacgacg gcaggagttg cattggtcct gccgccaaat cccgtgactt cgcaaaacac
     3901 gtcgtacagt ggcaccaggg cgtagccgaa agcgaacata ccgagggcgc acagaagcaa
     3961 ccgcagcgcc agcgctccgg agtcctggtt cacagttgtc ttcttgtcgt tcaatttccc
     4021 ttatgccctg cccacagtca ggaagatgaa gcccaggaaa atggcaaccg cgatcgcggc
     4081 cagccacagt gctgatcttc gaacgctttc cggggagcgg cggtcgcggt ttccgctcat
     4141 gagaatccag gggctggctc ggcagaaacc ttaggcggga cctcgaacgt gtggaatggt
     4201 gccggcgagg gcacagtcca ctccagtcct ttcgggttat cccacacttc cgcggtagct
     4261 ggttcgccgc ggccgcgcat gcaactgatt actacgtata cgaagatcaa ctgcgaaagt
     4321 ccgaagccga acgcgccgat gctcgcgacg aagttccagt cggtgaactg ggtggcgtag
     4381 tccgggattc tcctcggcat tccggcgaga ccgaggaagt gcatcgggaa gaacgtcacg
     4441 ttgacgaaga tggccgtgag ccagaagtga atctgcccga gcttttcgtt gtacatgcgg
     4501 ccggtccact ttggcaacca gtagtaggtg gcgcccagga tcgcgaacaa cgctccaggc
     4561 acgagcacgt agtggaagtg cgccacgacg aagtacgtgt cgtgatactg gaagtcggcc
     4621 ggcacgatcg cgagcatcag gccggagaaa ccgccgatcg tgaacaggaa caggaacgac
     4681 agcgcgaaga gcatcggcgt ctcgaaggtc atcgagctct tccacatcgt cgcggtccag
     4741 ttgaacacct tcacgcccgt cggaatggcg atgagcatcg tcgacagcat gaagaagagc
     4801 tggcccgcga gcggcatgcc cacc
//
LOCUS       DASUOM010000187         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_574027,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000187 DASUOM010000000
VERSION     DASUOM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574027"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1895)
                     /gene="gshA"
                     /locus_tag="VFY27_03945"
     CDS             complement(312..1895)
                     /gene="gshA"
                     /locus_tag="VFY27_03945"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX5764693.1"
                     /translation="MTTLFDRRIKRLVNSSPADTLRGGLKGVEKESLRVRPDGMLSDG
                     PHPPALGSPLTNRYITTDFSEALLEFVTPAFGTTWETLHFLCDIHQFSYERMDDELLW
                     ATSMPCKLPAESQIPLARYGRSNVGRMKTIYRRGLGYRYGRLMQTIAGVHFNYSLPEP
                     FWPLFREHEQRTETLQAFRSEAYLGLVRNFRRFGWLVLYLFGASPAVCKSFAGATAGL
                     ARLDGETLYEPYGTSLRMSDLGYSNRTQSKINISLNDLDGYIAGLVEAIRSPEPAFEK
                     IGLEPRGDADEGDYRQLSVNRLQIENEYYSSIRPKRVAYSGERPTEALRRGGVEYVEI
                     RSIDLNVFDPVGVNQNVMRFMEAFLIYCLLAESAPLDDAGLDEAARNQSLVARRGRDP
                     GLKLTREGEDLSLSGWGSQIVEGVCAVAELLDRGEDGNEYGQAVDAQAELLAHPDATP
                     SARLLQELHDRRSGFFAFAMDAARGHKEYFSALVSPNPDHLQHLEFEALDSHRRQKEI
                     ENSDTLSFEEYLANYFAGA"
     gene            2083..2472
                     /locus_tag="VFY27_03950"
     CDS             2083..2472
                     /locus_tag="VFY27_03950"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEX5764694.1"
                     /translation="MNRLPRFQPTLLLASALGLASSPVHALRCGGKLVLEGMHEVEVL
                     EHCGEPADVRDLGFIVRSFHPLAHRPPHGGVLFRYGPGNYYQEVLVTEYIYNFGPRKL
                     MRRLRFEGGMLADIETLGYGYLEKKRN"
BASE COUNT          550 a          835 c          812 g          522 t
ORIGIN      
        1 acaccaacga cgcctcacga tgctgatcca ctgccggaac gaagtacaac aggcacgcga
       61 tcagcgccat gcccgagaat atcccgatga cgaacaactt gaacacaagc ggccccgcaa
      121 ttgctgttgg ttttatcgag ctgctcaagc agccgggtta gcgaaagtct agttaacctg
      181 ctccgctctc acatcacgtg acgagagatt tgtgaaacag cgcacagtct gcgcaggcgc
      241 atcggcgaca tgtcacagtc tcacggcgac gcctggatgt gtatgtgcca gccggcagga
      301 ggacagcgga ttcaggcgcc cgcgaagtag ttcgccaggt actcctcgaa actgagcgtg
      361 tcggaattct cgatttcctt ctggcgccgg tgggaatcaa gggcttcgaa ttccaggtgc
      421 tgcaggtggt ccgggttcgg cgaaacgaga gccgagaaat attccttgtg accgcgcgcg
      481 gcgtccatcg cgaatgcgaa gaacccgctc cgtcgatcgt gcaattcctg cagcagtcgc
      541 gccgacgggg tcgcatccgg atgcgccagc aattcggcct gggcatcgac agcctggccg
      601 tattcgttcc cgtcctcgcc gcgatccagg agttccgcca ccgcgcacac gccctccact
      661 atctggctgc cccagccgga aagcgacaga tcttcgccct cgcgcgtcag cttgaggccc
      721 gggtcgcgcc cgcgccttgc caccagtgac tggttcctgg cggcttcgtc cagtcccgca
      781 tcgtccagcg gcgcgctttc ggccagcaag cagtagatca ggaatgcttc catgaagcgc
      841 atgacgttct ggtttacgcc gacggggtcg aacacgttga ggtctatcga gcggatttcc
      901 acgtactcaa cgccgccacg tcgcagggct tccgtcggcc gctcgccgga ataagcgact
      961 ctcttcgggc ggatcgagct gtaatactcg ttctcgatct gcagccggtt cacactgagc
     1021 tgccggtagt caccctcatc cgcatcgccg cggggttcga ggcctatttt ctcaaaagcc
     1081 ggttcggggc tgcggatagc ctccacgagg ccggcgatat acccgtcgag gtcattgagc
     1141 gaaatattga tcttcgactg tgtgcggtta ctgtatccga gatcgctcat gcgcaacgaa
     1201 gtgccataag gctcgtaaag cgtttcgcca tccagcctgg ccagcccggc tgtcgcgcca
     1261 gcgaaagatt tgcaaaccgc gggagacgcc ccgaaaaggt agagcaccag ccagccgaaa
     1321 cgtcgaaagt tccggacgag cccgaggtat gcctcggaac gaaacgcctg caacgtctca
     1381 gtgcgctgtt catgctcccg gaacaagggc cagaacggtt ccggcaacga gtagttgaag
     1441 tgcacgccgg cgatagtctg catgagccgg ccatagcggt acccgaggcc gcgcctgtaa
     1501 atggtcttca tacggcccac gttggaacgg ccatatcgtg cgagcggaat ttgcgattcc
     1561 gccggtagct tgcagggcat gctggttgcc cacaacagtt cgtcatccat ccgctcgtag
     1621 ctgaactggt ggatgtcgca caggaagtgc agcgtctccc aggtggtgcc aaaggccggc
     1681 gtcacgaact cgagcagcgc ttcagaaaag tcggtggtga tgtaccggtt cgtcagcggc
     1741 gaaccgagcg cgggcggatg cgggccgtcc gacaacattc cgtccggccg cacacgcagg
     1801 gattcctttt cgacgccctt gagcccgccg cgcagcgtat ccgccgggct cgaattcacc
     1861 agcctcttga ttcgtcggtc gaataaggtc gtcaatcggg acactccggc aaaccggatg
     1921 ggggctggtt tcgggacgtc atcatgctgc cacaggtgcc ccgaacgccg gactatacag
     1981 gcgcctctta tacctgccaa cggcatggct gaggccgatg ctttaatatg aaccaccgac
     2041 gcgcacagcg agtcccacca ggtaatgttt ctgacatccc gaatgaacag actcccgcga
     2101 ttccagccga cgctcctgct cgcttccgct ctcggtctcg cgagcagccc cgtgcacgcg
     2161 ctgcggtgcg gcggcaaact ggtgcttgag ggtatgcacg aagtggaggt actggagcat
     2221 tgcggcgagc ctgctgacgt acgggacctg ggtttcatcg ttcgttcgtt tcatccgctc
     2281 gcacacaggc ctccgcatgg cggcgtgctg tttcgttacg gaccgggaaa ctactaccag
     2341 gaagtgctgg tcaccgaata catctacaac ttcggtccca ggaagctgat gcgacggctg
     2401 aggttcgagg gcggcatgct ggccgacata gaaaccctcg gatacggcta cctggaaaag
     2461 aagcgcaact agtggcctgt aacagtgaaa tcgggactgg catcgcgacg ggatggtggt
     2521 ggctggcgcc gctctggagc gaagctgata gccgtagcta tcggctgagt gaagagcaag
     2581 ccaggcgccg ccagaccggg cgagacagtt cggatttcac tgttacaggc cactagctgc
     2641 gattatcgca attgcgaccg catcacaaat ctcgcacgcc tatacgttta tcattagccc
     2701 cggtgctacc gccggcggc
//
LOCUS       DASUOM010000188         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1601587,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000188 DASUOM010000000
VERSION     DASUOM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601587"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2637
                     /gene="clpB"
                     /locus_tag="VFY27_03955"
     CDS             58..2637
                     /gene="clpB"
                     /locus_tag="VFY27_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5764695.1"
                     /translation="MRMDKLTSKFQMALGDAQSLAVGQDHQFIEPVHLMVALLDQQGG
                     SVRGLLTKAGVNVNLLRSQVGLALDRLPKVEGTGGEVHIGNDLTKLLNLTDQLAQKRN
                     DQYISSELFVLAAMDDRSPLVKIMEQAGAVRGAVEKAIDELRGGQQVNDPNAEETRQA
                     LEKYTIDLTERAEQGKLDPIIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNNEIPEGLRGKRILSLDMGALIAGAKFRGEFEERLKAVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGSMDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPNVEDTIAILRGLKERYEVHHGVEITDPAIVAAATLSHRYISDRQLPDKAID
                     LIDEAASRIRIEIDSKPEAMDRLERRIIQLKIEREALTKEPDEASRKRLSDIEEQLND
                     LEKQYSDLEEIWKAEKAAMQGTTHIKEELERARLELETAHRAGDLARMSELQYGRIPE
                     LEKQLEAAVQAENRETTLLRNNVTEEEVAEIVSKWTGIPVSKMLEGEKEKLLAMEDKV
                     QERVVGQEEAVTAVADAIRRSRAGLSDPKRPIGSFLFLGPTGVGKTELTKALANFLFD
                     TDDAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLGSQMIQELAGEQNYEAMKSA
                     VMDIVSQHFRPEFINRVDDVVVFHPLGREHIRKIVDIQLGYLRQRLADRDMEIVLSDA
                     ARDKLAEAGFDPVYGARPLKRAIQQRVENPLAQEILKGKFKPGDTIEVAVAENALEFQ
                     LAA"
BASE COUNT          602 a          817 c          867 g          411 t
ORIGIN      
        1 ttaaatgacc acatcgaagc ccacccggtc gggtcgataa cctttaggcg aaccgctatg
       61 cgaatggaca aacttaccag caagtttcaa atggccctgg gcgatgccca gtcgcttgcc
      121 gtgggacagg atcaccagtt catcgagcct gttcacctga tggtggcatt gctcgaccag
      181 cagggcggtt ccgttcgcgg gctcctgacc aaggccggtg tgaacgtcaa tctcctgcgc
      241 tcgcaagtcg ggctggcgct ggatcgactg cccaaggtcg agggcaccgg cggtgaggtg
      301 cacatcggca acgacctcac caagctcctg aacctcaccg accagctggc gcaaaaacgc
      361 aacgaccagt acatctccag cgaactattc gtcctcgcgg ccatggacga tcgttccccg
      421 ctcgtcaaga tcatggagca ggccggcgca gtgcgcggtg ctgtcgagaa agctatcgac
      481 gagcttcgcg gcgggcagca ggtgaacgac ccgaatgcgg aagagacgcg ccaggcgctg
      541 gaaaaataca ccatcgacct gaccgaacgc gcggagcagg gcaagctcga cccgatcatc
      601 ggccgtgacg acgagatccg acgtactatc cagatcctgc agcgccggac caagaacaac
      661 cccgtcctga tcggcgagcc tggtgtcggc aagaccgcaa tcgtcgaggg tctcgcgcag
      721 cgcatcgtca acaatgagat acccgaaggc ttgcgcggca agcgcatcct gtccctggat
      781 atgggtgcgc tcatcgccgg ggcgaagttc cgcggcgaat tcgaagaaag gctcaaggcc
      841 gtccttaatg acctggcgaa acaggaaggc cagatcatcc tgttcatcga cgagctgcat
      901 accatggtgg gcgccggcaa ggcggaaggc tccatggacg ccggcaacat gctgaagccg
      961 gcgctcgcac gcggcgagct ccactgcgtt ggcgccacga cactcgacga ataccggaaa
     1021 tacgtggaaa aggacgccgc cctcgaacgg cgcttccaga aggtgctggt cgatgagccg
     1081 aacgtggaag atacgatcgc catcctgcgg ggcctgaaag agcgttacga ggtacaccac
     1141 ggcgtcgaga tcacggaccc cgcgatcgtc gcggctgcga cactgtccca ccgctacatc
     1201 agcgaccgcc agctcccgga caaggcgatc gacctcatcg acgaagcggc ctcccgtatc
     1261 cgcatcgaga tcgactcgaa gccggaggcg atggacaggc tggagcgccg catcatccag
     1321 ctcaagatcg agcgtgaagc gctgacgaaa gagcccgacg aggcgtcccg caagcggctg
     1381 tcggacatcg aagaacagct gaacgacctg gagaagcagt attcggatct cgaggagata
     1441 tggaaggccg agaaggccgc catgcagggc acgacgcaca tcaaggaaga actggagcgg
     1501 gcccggctcg agctggaaac ggcgcaccgt gccggcgacc tcgcgcgcat gtccgagctg
     1561 cagtacggac gcatccccga gctggagaag cagctggagg cggccgtgca ggcggagaac
     1621 cgggaaacca cgctgctgcg gaacaacgtg accgaggaag aggttgcgga aatcgtctcg
     1681 aagtggaccg gaatcccggt ctccaagatg ctggagggcg aaaaggaaaa gctgctcgcg
     1741 atggaagaca aggtgcagga acgcgtggtc ggccaggagg aggcggttac tgccgtcgcc
     1801 gacgcaatac gacgctcccg cgcgggactt tccgacccga agcggccgat aggctcgttc
     1861 ctgttcctgg gccccaccgg cgtcggcaag acggagctga ccaaggcgct cgcgaacttc
     1921 ctgttcgaca ccgacgacgc aatggtacgc atcgacatgt ccgaattcat ggagaagcac
     1981 tcggtggcgc gtctcatcgg cgctcctccc ggatacgtgg gttacgagga gggcggatac
     2041 ctgaccgagg ccgtccggcg ccggccctat tcggtgatcc tgctcgatga agtcgagaaa
     2101 gcgcacccgg acgtgttcaa cgtgctgttg caggtgctcg atgacgggcg cctcactgac
     2161 ggtcagggca gaacggtcga ctttcgcaat accgtgatcg tcatgacctc gaacctggga
     2221 tcgcagatga tccaggagct ggcgggcgaa cagaactacg aggccatgaa gtccgcggtg
     2281 atggacatcg tctcccagca cttcaggccc gaattcatca atcgcgtgga cgacgtggtg
     2341 gtattccatc cgctgggccg cgaacacatc cgtaagatcg tggatatcca gctcgggtac
     2401 ctgcgccagc ggcttgccga ccgggacatg gagatcgtcc tgtccgatgc cgcaagggac
     2461 aagctggcgg aagccgggtt cgacccggtt tacggcgcga ggccgctgaa gcgtgccatc
     2521 cagcagcgcg tggaaaaccc cctggcccag gagatactga agggcaagtt caagccgggc
     2581 gacacgatcg aggtggcggt agccgagaac gcgctggagt tccagcttgc tgcgtaacgg
     2641 gaagatccct gaaccatggc ccaagcgact gtaggagctc gccatgcgag cgagttc
//
LOCUS       DASUOM010000189         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_353711,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000189 DASUOM010000000
VERSION     DASUOM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353711"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..406)
                     /locus_tag="VFY27_03960"
     CDS             complement(122..406)
                     /locus_tag="VFY27_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764696.1"
                     /translation="MFNTINLEDGDKVDFWMLTDDAFDASRFARRVTVDVFGTLLKVS
                     SPEDTVLAKLRWSKLSGGSEKAFIDALRVFEVQAHALDRAYLVEWVEALE"
     gene            469..645
                     /locus_tag="VFY27_03965"
     CDS             469..645
                     /locus_tag="VFY27_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764697.1"
                     /translation="MAEKGRGILRADLDDDTQIVRSPGLPLKTRSDRSGDHIRDAVGI
                     ENLNHEPEGIERGH"
     gene            complement(1040..2095)
                     /locus_tag="VFY27_03970"
     CDS             complement(1040..2095)
                     /locus_tag="VFY27_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5764698.1"
                     /translation="MDATLHSRFHVAAGAAAAFDDFIEDLVVGLDQQGLTFVPGPSGQ
                     VLAGSNVVAAVLEWQPGERMVLAWTESAPESAAVEIELQCAAGAAGTEVSVHIRNWAA
                     ALGSWGDVSGWFASQVVAPVIGALTPARAGDWVTDRVARHPSGRQSRTTYRDPLFHYP
                     NFRVILEELALTPNDVLLDVGCGGGALLHESLKTGCRAAGIDHSLEMVRVARELNRAA
                     VAEARVEILHGRADQLPFEASSFTCATMTGVLGFLAHPEECLAEIWKVLKPAGRFVML
                     GSDARLRGTPAAPEPIASRLRFYEDDDLERMGRAAGFEHVVVIRRDLSPHARTVGIPA
                     EYIGLFEGLTSFLVARK"
     gene            complement(2301..2657)
                     /locus_tag="VFY27_03975"
     CDS             complement(2301..2657)
                     /locus_tag="VFY27_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5764699.1"
                     /translation="MDKRKQERLKAGGWRAGSTAEFLELTLEEAEFVEMKLALSSTLR
                     VRRTDQGWSQDVLADKIGSSQSRVAKMEASDPSVSVDLLIRGLLATGASKDDIASAIV
                     GTRPKRSGRAKSRSNA"
BASE COUNT          535 a          875 c          753 g          524 t
ORIGIN      
        1 acccggttat gcgcagtatt ctgccgcctg tacgatgcca acctgtaccg gccggtcgtt
       61 gcccgcgccg aggttcttac ggcagttcag cctccgcctc taagcggcgc caaagctccg
      121 ttcactccag tgcctccacc cactcaacga gatacgcccg atccagagcg tgcgcctgca
      181 cctcgaacac acgtagtgca tcgatgaacg ccttctcgct accaccggag agcttcgacc
      241 atcgaagctt ggccaggacc gtgtcttccg gacttgagac cttcagcaac gtcccgaaca
      301 catccacagt tacgcggcgc gcaaagcgtg aggcatcgaa cgcgtcatca gtcagcatcc
      361 agaaatcgac cttatcaccg tcttcgagat tgatcgtgtt gaacatcccg cctgtttcaa
      421 tggcttcaag aatcgcgtgc tcatcaaggt agaactgtgg ccgtttgaat ggctgagaag
      481 ggcagaggca tcctgcgcgc ggatcttgac gacgacaccc agatcgtgcg tagccctggg
      541 ctccccctga agactcgaag cgaccgatcc ggtgaccata tacgcgatgc cgtgggaatc
      601 gagaacctga accacgaacc ggagggtatc gagcggggtc actgactact cagcccgttc
      661 ccgcgccttc ttcatgcggt cgaggaggag gcggttcagg tctgatgggg agagctcggg
      721 gtggagattc tccagacctc gcttcgtaag agctctggag aattccgaca gctcaaacgc
      781 cttcttcaag cggttttccg accccatctc ccggaggacc tgtacataga ctccgtggtt
      841 tgggcgctct ttcatagtcc ccaatctggt ctggcgggac tccccccaca atacggcaga
      901 atattgcgta taacgtgata cgatttgcct gcctcgttct tcagcctgca gcgccgcgcc
      961 gcgtgagccg cgtgaatagg atgtcaggtg aaggccggcg ttgtctggct atgcgatagg
     1021 cctggacact cgcgtccgat tacttcctgg caaccaggaa cgacgtcagg ccttcgaaga
     1081 gtccaatgta ctccgccggg attcccacag tgcgggcgtg aggactcagg tcgcgccgaa
     1141 tcacaacgac atgctcgaag ccggctgcac ggcccatcct ctccagatca tcgtcctcgt
     1201 agaaacggag gcgggacgcg ataggttcgg gtgcagcggg cgtaccgcgg agtcttgcat
     1261 cactgccgag cattacgaag cgacctgccg gcttcaggac cttccagatt tccgcaaggc
     1321 attcctcagg gtgggccagg aagcctagca cgccggtcat ggtcgcgcac gtaaatgagc
     1381 tcgcctcaaa tggcagttga tccgcgcgcc catggaggat ctctactcgg gcctcggcca
     1441 ctgctgcgcg gttcaactcg cgagccaccc ggaccatctc gagactgtgg tcgatgcctg
     1501 cagcccggca gcctgtcttc aaactctcgt gcaaaagggc gccaccgccg caaccaacgt
     1561 ccaggagaac gtcattcggt gtcagcgcca gttcttcgag aatcacccgg aagttggggt
     1621 agtgaaagag gggatctctg taggtcgtgc gcgactgccg tccgcttggg tgacgagcga
     1681 cgcgatcggt cacccagtct cctgcccttg caggcgtcag ggcgccgatt acgggcgcga
     1741 caacctgtga tgcaaaccag ccagacacat ctccccacga ccccagtgca gccgcccagt
     1801 tccggatgtg caccgacact tcagtgccag ctgcgccggc tgcgcactgg agttcgattt
     1861 ccaccgctgc ggattcggga gctgattccg tccacgcaag caccatgcgc tcgccaggct
     1921 gccactccaa cactgccgcc actacattcg aacctgccag aacttgacca ctcggaccgg
     1981 gtacgaacgt gaggccctgc tggtcgagcc cgactaccag gtcctcaatg aaatcatcga
     2041 acgctgccgc tgcacctgcc gccacgtgga atcggctgtg gagagtcgcg tccattgtca
     2101 atgcttggcc acaagttgat cgtgtttgcg cctgtgcatc acgagcgaaa gccggctttc
     2161 acctaacgtg atacggttgc cgacgtgccg cagcgaggaa accggtgagc gaagcgatcc
     2221 ggcgcctgaa cggaggcatg tcccgaaggg caagccgcgc gtagcaaagc ggagcgcggc
     2281 gcagcggcaa gagtcagtta tcaggcgttg ctgcggctct tggcccggcc tgaccgcttg
     2341 ggacgagtcc caacgatagc cgaggcgatg tcgtcctttg aggcccccgt ggcaagcaat
     2401 ccgcgtatca gcaaatcaac cgacaccgac gggtcgcttg cctccatttt cgctacccgc
     2461 gactgactcg acccgatctt gtcggctagt acgtcttgcg accagccttg atcggttcga
     2521 cggacccgca acgtcgaact cagtgcgagc ttcatctcca caaactcggc ttcctccagc
     2581 gtgagctcca gaaactcagc cgtgctccct gctctccacc caccagcctt cagccgctcc
     2641 tgcttcctct tatccatctc aacctccact tagctggtcg tactgct
//
LOCUS       DASUOM010000190         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_158434,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000190 DASUOM010000000
VERSION     DASUOM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158434"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1305)
                     /locus_tag="VFY27_03980"
     CDS             complement(91..1305)
                     /locus_tag="VFY27_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5764700.1"
                     /translation="MRIHQQDSKTALVLPGGGARGAFQVGVLKALAELVPSRSANPFP
                     IISGTSAGAINSVVLASKARHFPLAVKELERVWGRFESGHVFRTDSLTMLKSSLHWAA
                     AIVLGGFLVGMPRSLLDNSPLRALLSRNIRFPRIQKSIEDGNLHAVAVTAAGYGSARS
                     MSFFQGAPGIRSWSRTRRIGVRQALHLDHLMASIAVPLIFPPVLIGNEYFGDGAMRQA
                     TPLSPAIHLGADRILVIGVRDETADASLLDAQPQRYPSFAQIAGYMLDTLFMDGLYSD
                     LERMTKINQLIDAVPPDQRTGPVKRMRAIDTMLIVPSRDLREIAHEHRRDMPVPLRIL
                     LRGIRGHSKPENRLLSFLLFEQSYTQALIDLGYKDAMAVRDNLLDFVTGADVPRLFAP
                     AWVKKDLSGFDF"
     gene            complement(1376..>2531)
                     /locus_tag="VFY27_03985"
     CDS             complement(1376..>2531)
                     /locus_tag="VFY27_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5764701.1"
                     /translation="YLTLLDAMNRGLAGRSVDDFYFLARSSLVKDETLFDRFDRIFSA
                     HFRGIEDALHSLLGDVPDDWLQRQAELYLSPEERRQIEAMGGFDKLMEALRERLTEQG
                     ERHEGGNKWIGTAGTSPFGAYGFNPEGVRIGQQGSRHRRAVKVWDRREYRNLDDTVEL
                     GTRNIKIALRKLRKFAREGAADQLDLDDTIDKTARNAGLLDLRMVPERHNAIKVLLCL
                     DIGGSMDDHVRVCEELFSAAKSEFKHLEYFYFHNFIYESMWKNNRRRHSERIDTSSIA
                     HKYSADYKLIFVGDATMSPYEIVYAGGSVEHWNKEPGAVWIKRLLNTFPKAIWLNPEP
                     EQRWDYTPSIKLTRELMDDRMFPLTVSGLDAGIRRLLHGTAGTASARVGH"
BASE COUNT          475 a          789 c          759 g          508 t
ORIGIN      
        1 ggcgtcgtac tcatggcgat aaccgacgtc gccgggtaca tgtccttcct cggtctcggc
       61 accctcctgc tgctataggc cctacgctct ttagaaatcg aatccgctca gatctttctt
      121 tacccaggct ggcgcgaaca gccgcggcac atcggcaccc gtcacgaaat caagaaggtt
      181 atccctgacg gccatggcgt ccttgtaccc gaggtcgatc aacgcctggg tataggactg
      241 ctcgaacagc aggaaactca acaggcggtt ctccggctta ctgtgcccgc gtatcccgcg
      301 caacaggatg cgcaacggca ccggcatgtc gcgacggtgc tcgtgggcta tctcgcgcag
      361 atccctgctc ggcacgatca gcatcgtatc gatggcccgc atacgcttca ccggacctgt
      421 ccgttggtcc ggcgggacgg catcgatgag ctggttaatc ttggtcatcc tttcgaggtc
      481 ggaatagagg ccgtccatga aaagcgtatc cagcatataa cccgcgatct gggcgaagct
      541 cggatatctc tgcggttggg cgtccagcaa ggacgcatcc gccgtttcat ccctgacgcc
      601 gataacgagt atccggtcgg ctcccaggtg aatggccgga ctcagcggcg ttgcctgccg
      661 catggcccca tcaccgaaat actcgttacc gatcagtacc ggtggaaaga taagcggtac
      721 tgcgatgctt gccatcaggt ggtccaggtg cagggcctgc cggaccccga tccggcgtgt
      781 gcgcgaccag gaccggattc ctggcgcacc ctggaagaag gacatcgaac gagccgagcc
      841 gtagcctgct gcggtcaccg ccacggcgtg gaggttgccg tcctcgatgg acttctggat
      901 gcgcggaaag cggatgttcc ggctgagcag cgcacgcaac ggcgagttgt ccagcaacga
      961 tctgggcata ccgaccagga agccgcccag cacgatggcc gccgcccagt gcaggctgct
     1021 tttcagcatc gtcagggaat ccgtgcggaa gacatgacca ctttcgaaac ggccccagac
     1081 gcgctccagt tccttgaccg ccaatgggaa gtggcgcgcc ttgcttgcaa ggacgaccga
     1141 gttgatggcc ccggccgacg taccgctgat gatggggaag ggattggccg acctggatgg
     1201 caccagttca gccagcgcct tcaggacgcc cacctggaac gcgccgcggg cgccgccgcc
     1261 cggcagcacc agcgccgtct tcgagtcctg ctgatgaatt ctcattcttg cggcttgttc
     1321 cagcccggtt cttcaggcag cgcttgcgtg ctgcgggaaa acttctattc agcagtcagt
     1381 ggccgacccg ggcggaggcc gttccggcgg tgccgtgcaa caggcgcctg atgccggcat
     1441 cgaggccgct caccgtcagt ggaaacatcc ggtcatccat cagttcccgg gtcagcttga
     1501 tggacggcgt gtagtcccag cgctgctccg gctccggatt gagccagatc gctttcggga
     1561 acgtattcag caagcgtttt atccagacgg cgccaggctc cttgttccag tgctcgacac
     1621 ttcctccggc gtagacgatc tcgtacggac tcatggtcgc atctccgacg aagatgagtt
     1681 tgtaatccgc agagtacttg tgggctatgg aggaagtatc gatgcgctcg ctgtgccgtc
     1741 gccgattgtt cttccacatg ctctcataga tgaaattgtg gaagtagaag tactcaaggt
     1801 gcttgaactc gctcttggcg gccgaaaaga gctcttcaca cacgcgcacg tgatcgtcca
     1861 tggacccgcc gatgtcgaga cacagaagaa ccttgatggc gttgtgtcgc tccgggacca
     1921 tgcgcaggtc gaggaggccg gcattgcgcg cggtcttgtc gatcgtatcg tcgagatcga
     1981 gttggtcggc cgcgccctcc ctcgcgaact tgcgaagctt gcgcaacgcg atcttgatgt
     2041 tgcgcgttcc gagttcgacg gtgtcgtcga ggttacgata ctccctgcga tcccatacct
     2101 tgacggcgcg gcgatgacga gagccctgct gtccgatgcg cacaccctcg ggattgaagc
     2161 catacgcccc gaacggggac gtgccggccg tgccgatcca cttgtttcca ccctcgtgcc
     2221 gctcgccctg ttcggtaaga cgctcgcgca gtgcttccat cagcttgtcg aagccaccca
     2281 tcgcctctat ttgcctgcgc tcctcgggcg acaggtaaag ctccgcctgt cgctgtaacc
     2341 agtcgtccgg cacgtcgccg agcaggctgt gcaatgcgtc ttcgatgccc cggaagtggg
     2401 cggaaaatat cctgtcgaaa cgatcgaaca gcgtttcgtc cttcacgaga gaagatcgag
     2461 cgagaaagta gaagtcatcg acgctgcgcc cggccaggcc cctgttcatc gcgtcgagca
     2521 gagtgaggta c
//
LOCUS       DASUOM010000191         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1419290,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000191 DASUOM010000000
VERSION     DASUOM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419290"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VFY27_03990"
     CDS             <1..1698
                     /locus_tag="VFY27_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764702.1"
                     /translation="PQEEEVASLPGGLNYYLTWIKNMGCVGCHQMGNRATRTMPEMFA
                     DIEPSHQAWARRIASGQAGNSMVRIAADLLEGLPLKYLAEWTDSIEAGAIPAEVPDRP
                     AGIERNVVATVRDWSEPQYYMHDLSGTDRRDPTVNAFGPLYGAPELSTDEMPILDPVR
                     NTSTVYEVPVRDEDTPTTNEDPVIAPSPYWGEERIWDSRANIHNPMLDQDGRVWLTAS
                     IRDEDNPDFCKQGSDHPSARLYPKESSSRHLAVLDPASGDYTFVDTCFSTHHLQFAYD
                     ERNTLWTSGGGDVVGWLDTTRFLATGDAAASQGWAPLILDTNGNGQQDPWSEPDEDPD
                     PELDMRVPNGFYAVMPEPRGAAVWGSNAFRYPGSITRLQPGNDPPQTALAEVYYPPLP
                     GFGVRGADIDKDGVVWTSLGSGHLGEFDRRKCRRPLNGPEATGNHCPEGWTLHDLPGP
                     GFADLPGFSVESSYYTWVDQHNSLGLGDDIPIATGNLFDGVHALVEGEFVTLRIPYPL
                     GFYTKGFEGRIDDPDIGWKGRGIWVPEGGRTPWLKEDGKGSKPLVVHFQVRPDPLAK"
     gene            1733..>2519
                     /locus_tag="VFY27_03995"
     CDS             1733..>2519
                     /locus_tag="VFY27_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5764703.1"
                     /translation="MLTENKQKVTVAAGRTRGSVVGRSLEAEAIAAALSGLENGRGHA
                     LYLAGEPGIGKSTLARLAASEAETRGIPVFWGFAWETGGAPAYWPWTQLLRPLIERFP
                     ASPEVRGKLAQILPESAGEGDLHLQPEQARFLLLEAVRALLASVSRQSPLMLIFEDLH
                     AADNDSLQMLQHVTKHLRSMPVLLLGTFRELDARTSQETGPLWQSVRDAEVLKLARLA
                     ENEVREYLLSTGAAVDDEFVRILVGTTAGNPLFVEELAEVFARQ"
BASE COUNT          494 a          813 c          787 g          425 t
ORIGIN      
        1 ccgcaggagg aagaggtagc aagcctgcct gggggtctca actactacct cacctggata
       61 aaaaacatgg gttgcgtcgg ctgtcaccag atgggcaacc gcgcgacccg cacgatgccg
      121 gagatgtttg cagatatcga accatcgcac caggcctggg cccgccgcat cgcctcaggt
      181 caggccggca acagcatggt gcggatcgcc gccgacctgc tggagggcct gcccctcaag
      241 taccttgcgg aatggacgga cagcatcgag gccggggcga tacccgcgga ggttcctgac
      301 cgccccgccg gaatagagcg caacgtcgtt gcgaccgtgc gcgactggtc cgaaccacag
      361 tattacatgc acgacctgtc cggcacggac cgtcgcgatc cgacggtaaa tgccttcgga
      421 cctctttacg gcgcgccgga actcagcacg gacgagatgc ccattctcga cccggtccgg
      481 aatacgtcaa ccgtttacga agtaccggtg cgcgacgagg acacgccgac gacaaacgaa
      541 gatcccgtga ttgcgccctc cccttactgg ggcgaggagc gcatctggga cagccgcgcg
      601 aacatccata acccgatgct cgaccaggac gggcgcgtgt ggctcactgc aagtattcgc
      661 gacgaagaca atccggattt ctgcaagcaa gggtcggatc acccgtcggc aaggctttac
      721 ccgaaagaga gttcgtcgcg gcaccttgcg gtactcgatc ccgcgagcgg cgactacacg
      781 ttcgtcgaca cctgcttcag cacgcatcac ctgcagttcg cctatgacga gcgcaatacg
      841 ctctggacca gcggcggcgg tgatgtcgtc ggctggctgg acaccacccg cttccttgca
      901 accggggatg cagcggcatc acagggatgg gcgccgctca ttctcgatac caatggcaac
      961 gggcagcagg atccatggag cgaaccggac gaagatcccg atccggaact cgatatgcgc
     1021 gtgccaaacg gtttttatgc ggtcatgccg gaaccgcgcg gcgctgccgt ctggggctcg
     1081 aatgcctttc gctatcccgg ctcgatcacg cgcctgcagc cgggcaacga tccgccacag
     1141 acagcgctcg ccgaggtcta ctacccgccg ctaccgggct tcggagtccg tggcgccgac
     1201 atcgacaagg acggcgtcgt gtggacatcg ctcggcagtg gtcacctggg cgagttcgat
     1261 cgccgcaagt gcaggagacc gttgaacggt ccggaagcaa cgggtaacca ctgccccgaa
     1321 ggctggacgc tgcacgacct gcccggtccc ggcttcgccg acctgcccgg cttcagcgtc
     1381 gagtccagct attacacctg ggtcgaccag cacaactccc tcggtcttgg cgacgacata
     1441 ccgatcgcga ccgggaacct cttcgacggc gtgcacgcgc tggtggaggg cgagttcgtc
     1501 acgctgcgca tcccctaccc gctcggcttc tatacgaagg gattcgaggg acgcatcgac
     1561 gaccccgaca tcggctggaa aggccgcggc atctgggttc cggaaggcgg ccgcacgccc
     1621 tggctcaagg aagacggcaa gggctcgaaa cctttagtcg tgcacttcca ggtccggccg
     1681 gaccctctcg caaagtaagg ttaccgcggc aaactctcta tacttgccgc tcgtgctgac
     1741 agaaaacaaa caaaaggtaa cagtcgccgc cggcaggacc cgcggatcgg tcgtcggccg
     1801 cagcctggaa gcagaggcta ttgctgctgc gctttcgggg ctcgaaaacg gtcgcggcca
     1861 cgcgctctac ctcgccggag aaccgggtat cggcaagagt acgctcgccc ggctggctgc
     1921 cagcgaagcg gagactcgcg ggattcctgt tttctggggc ttcgcctggg aaaccggcgg
     1981 cgctccggca tactggccgt ggacacagtt gctgcgtccg ctcatcgagc gctttcctgc
     2041 cagtccggaa gtgcggggca agcttgcaca gatcctgccc gagtccgcag gcgaaggaga
     2101 tcttcacctg caaccggaac aggcgcgctt cctgctgctg gaggcggtac gcgcgttgct
     2161 cgcgtcagtc agccggcagt cgccgctcat gctgatattc gaagacctgc acgccgccga
     2221 caatgattcc ctgcagatgt tacagcacgt aacgaagcac cttcgcagca tgccggtgct
     2281 cctgctcggg acgttccgcg aactcgatgc ccgtacttca caggagaccg gcccgctgtg
     2341 gcaaagcgtg cgtgacgccg aggtgctgaa gctcgcgagg ctcgctgaaa acgaagtgcg
     2401 ggaatacctg ctatcgacgg gagcggccgt ggacgatgaa tttgtccgga tactggtcgg
     2461 taccactgcg gggaatcccc tgttcgtcga agagcttgcc gaggtgttcg cgcgacaga
//
LOCUS       DASUOM010000192         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1677017,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000192 DASUOM010000000
VERSION     DASUOM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677017"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFY27_04000"
     CDS             complement(<1..372)
                     /locus_tag="VFY27_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764704.1"
                     /translation="MDSKKERRDRLVVLSFVSIMLMLVYGPLAEWFHAADHLLYDTIA
                     THVGNETLDDGVIVSVNPAQKSAQDVSHLYGQLVGRFQDMGAARIVITEGPQPLPSGE
                     LPGWAAALGNRTPMFVPRGHPF"
     gene            528..1871
                     /gene="radA"
                     /locus_tag="VFY27_04005"
     CDS             528..1871
                     /gene="radA"
                     /locus_tag="VFY27_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5764705.1"
                     /translation="MARTRTAFVCSDCGAHSVKWQGQCPDCEAWNTLVQTALAGTAPA
                     ATRSSPPRAMSDFGDDVESRHATGFAEFDRVLGGGLVPGSVVLLGGDPGVGKSTLLQQ
                     VAAQLPASLNTCYATGEESLRQVAQRSRRLGTNSSALKLVSDTSLDNILEHAAAAGAK
                     AVVIDSIQTVATHELPSAPGSVAQLRECVGRIVQFAKQRDVAFFLIGHVTKEGAIAGP
                     RVVEHMVDTVLYFESDPGSRYSIVRAVKNRFGASSEMGVFAMTEAGFREVRNPSAIFL
                     SQSPVASPGSIISVAWEGSRPLLVEIQALVAEAGGNYAKRLAQGVDQNRLALLVAVLH
                     RHGGISLAGEDIFVNVVSGLRIGETSADLPTVLAIVSSLKDRPCRKKMMSFGELGLAG
                     EVRPVRFGTERIAEAAKQGFNQAIVPKANVPRKTPAGIDVIGASTLEEALEHAFA"
BASE COUNT          413 a          645 c          712 g          385 t
ORIGIN      
        1 aaacgggtgg ccacgtggaa cgaacatcgg cgtacggttg ccgagagcgg cggcccaacc
       61 ggggagttca ccggagggta ggggctgcgg accttcagtg atgacgatcc tcgcagcccc
      121 catgtcctgg aaacgcccta cgagctgacc gtacaggtgg ctcacgtcct gcgcggattt
      181 ctgcgcggga ttgacgctga cgatgacgcc gtcatcgagc gtctcgtttc cgacatgcgt
      241 cgcaatcgtg tcgtacagca ggtgatcggc ggcgtggaac cactcggcca ggggtccata
      301 taccagcatc agcatgatgc tgacgaagct gagtacgacc agccggtccc ggcgttcttt
      361 cttgctgtcc aatgcaccgc tctctatata acgcgcggaa gtataagaaa tgatgtggct
      421 tcttagtgag atagggttgg cagtttttga gcgatgagag gcgcgcaagc gcggtgctat
      481 gatgccgctt ccaatccgga tacgaatcaa aggcaagcat cgaccgcatg gctcgaacga
      541 gaacggcatt cgtgtgcagc gattgcggcg cgcatagcgt gaaatggcag ggccagtgtc
      601 ccgactgcga ggcctggaac acgcttgttc aaaccgccct ggcaggcacc gcgcccgcgg
      661 caaccaggag ctctcccccg agggcgatgt cggatttcgg cgatgacgtc gagtctcgcc
      721 atgcgaccgg cttcgcggaa ttcgaccggg tgctgggtgg cgggttggta cccggctccg
      781 tagtgctgct gggaggagac ccgggggtgg gaaaatcaac gctgttgcag caggtcgcgg
      841 cgcagttgcc ggcctcgctc aacacgtgct acgccacagg cgaagaatcg ctgcgccagg
      901 tcgcccagcg ctcccgccgg ctcggcacca actcctcggc gctgaaactg gtttccgaca
      961 cgtcgctgga caacatcctg gagcatgcag cggcggccgg ggccaaagcc gtcgtcatcg
     1021 actcgattca gaccgtggcg acgcacgaac tgccttccgc accgggctcc gttgcgcagc
     1081 tgcgcgagtg tgtcgggcgc atcgtccagt ttgccaagca acgtgacgtc gctttctttc
     1141 tcatcgggca cgttacgaag gaaggcgcca tcgccggccc cagggtcgtc gaacacatgg
     1201 tcgataccgt gctttatttc gaaagcgacc ccggcagccg ttattcgatc gtccgggcag
     1261 tcaaaaaccg gttcggtgcg agcagcgaga tgggtgtgtt cgcgatgacc gaagccggat
     1321 ttcgcgaggt acgaaatcct tctgccatct tcctttcaca gtcgccggtc gccagcccgg
     1381 gcagcatcat atcggttgcc tgggaaggaa gccgtcccct gctcgtcgag atccaggcgc
     1441 tggttgcgga ggccggcggt aactacgcga aacgactggc acagggagtc gaccagaatc
     1501 ggctcgcatt gctcgtggcc gtgcttcacc ggcatggcgg gatctctctc gcgggcgagg
     1561 acatcttcgt caatgtcgtg agcggcttgc gcatcggcga gacatctgct gacctgccga
     1621 cggtgctggc aatcgtctcg agcctcaagg atcggccgtg ccgaaagaaa atgatgagct
     1681 tcggcgagct gggactggcg ggcgaggtcc ggccggttcg cttcggcacg gagcgtatcg
     1741 cggaagcggc gaagcaggga tttaaccagg caatcgtgcc gaaggcgaac gtgcctagga
     1801 aaacgccggc gggaatagac gtgatcggtg cgtcgactct cgaagaggcg ctggaacacg
     1861 cgttcgcttg aagcggagct tttgcggtaa cggcccctgt cgcccgtggg actggctgct
     1921 actaccttct ctcacgacaa tgcgggaggt acggatgata ccgctgcagg agcccgtccg
     1981 acgggcgacg ctcacagctg cgtgaagcct aggtccacgt gcggcccagg agctgttcga
     2041 ggatgtaacg gctgccgaag taggcaaggc cgagcgtcgc gaagccccac aggtagaggg
     2101 acaccgcgcg gcggccgcgc caaccggcga accgacgtcc cgccagcaat acccc
//
LOCUS       DASUOM010000193         7238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_756883,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000193 DASUOM010000000
VERSION     DASUOM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756883"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VFY27_04010"
     CDS             complement(<1..1906)
                     /locus_tag="VFY27_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5764706.1"
                     /translation="MKLTRGISTCCIALLCSFALAAENDSDKEDKNGWDVNSPPGDSR
                     QIPIHTSSGTWMSLDVSPDGRTIAFDLLGDIYLVPIEGGDARPIDSGHSWSMQPRFSP
                     DGSEIAYTSDAGGGDNIWIMPTDGSEKRQLTKEDFRLLNNPAWSPDGDYIAARKHFTT
                     TRSLGTGEIWLYHRNGGSGVAVVERPNEEHQKELGEPAFSPDGRYIYFSVDSTPGGTF
                     IYAQDSNDQIFEIRRHDLQENRTESFVTGAGGAVRPAPSPDGNYLAFVRRLEGKTALF
                     VKDLTSGTEIPLYDDLDQDMQEVWAVHGAYPNMDWTPDSQSIVFWAGGEIRRIDRESR
                     EVTEIPFDVDDTRTVFDPPRPTIDVAPDSFETRMVRNARVSPDGSQVVFESTGRLYIK
                     PVPDGEPRPLTRDSREHFQYFPSWSRDGNSIVYVTWSDTELSHVHQVRANGGRSTRLT
                     RKPGHYSNPRFSPDGATVVYEATEGGFLTAPEWSIDTGVFSVPVRGGESRQVTDNGSN
                     PHFGARGDRLYVTRDLDDAAALVSIDLNGKAARTHASGKYVTAYEVAPDDRHLAFREN
                     YHVYVLPFPPGGKPLELSTAVDSVSMTKASGDGGNWPHWSGNGETLNWSLGPTVFSAG
                     VDELFVLAKDT"
     gene            complement(1964..3670)
                     /locus_tag="VFY27_04015"
     CDS             complement(1964..3670)
                     /locus_tag="VFY27_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5764707.1"
                     /translation="MLRCAFRPIVITLVAIVSMAACSPPAEEAPSGPPAGVETEDRFS
                     SLSEITRENLQGHLSFLADDARAGRMTGTAGYDDAAAYVAQQFEALGLAPGGDEGWYQ
                     NVPMLGRRLDIESAAVTLHRDSGDAELEWKEDFVMGGDEVRGETSVTAEVVFAGYGVH
                     APEMNYSDYEDIDVRGKIVAVFGGAPERFPHNERAFYSSGRTKAEEMVRRGAIGRIGL
                     RSRVDQKRVPWERLTLNAGILQGMSWINLSGEVADYHPEIEGDATFSVPTAAELFEGA
                     PITFEEALDAADAGRPLSTPLGTEVTLSQKTRHEEVESPNVIGIMPGSDPELKDEYVV
                     FSAHLDHVGIGEPVDGDDIYNGYYDNAMGVSLLIEAARAFAAMPEPPPRSILFIALTG
                     EERGLIGSDYFAHYPTVPSDSLVANVNLDMPLLLYPLADVIAFGAEHSTLQQLIEPAV
                     AAEGFALTPDPVPEEVLFIRSDQYSFVRQGVPSVFLVPGFTSTDPQVDGAAAFQEHLA
                     THYHRPSDDSSRPVDWDSALRFARANVRIGLAVADADERPAWNEGDFFGDRFARPAST
                     SP"
     gene            3966..5504
                     /locus_tag="VFY27_04020"
     CDS             3966..5504
                     /locus_tag="VFY27_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX5764708.1"
                     /translation="MTENTKATLKDQVSQLVRHIEVESPELLGVVQSFRRLDRVAYKL
                     GLLDPTRSYATQIPWWPLISVLGTYSSGKSTFINNLLGQPLQKTGNQAVDDKFTVICY
                     GREQTPRVLPGLALDSDPRFPFYKISREIERVMEGEGARLDSYLQLKTCNSDRLRGKI
                     VIDSPGFDADAQRTSTLRITDHIIDLSDLVLVFFDARHPEPGAMRDTLQYLVSGTLQR
                     PDFNKFMHILNQIDNAAREDNPEEVFAAWQRALAQQGLTAGRFYRIYDPFASVPIEDE
                     HLKQRFESKRDEDMAEITRRIEELEVERSYRAVGRLEQLAKRIRDVVVPRLTAARRAW
                     RKRVLLLDALAFGTILVSFLVLSNMLGWWQGLRFQPPWLDTVERSPLLLWSGVVIVLL
                     AAWQLHRVLRRLAGRRIARQLRHDAALGDQANSVADAFDRNVRSFWPFVFANPRGWGI
                     WARRRLDAILNDAYAAVQQLNDRYTDPSGTRIAEEGDADEAPDFVPHPPPPAGRTGVE
                     GHAR"
     gene            complement(5542..7062)
                     /locus_tag="VFY27_04025"
     CDS             complement(5542..7062)
                     /locus_tag="VFY27_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5764709.1"
                     /translation="MNRPANIVDTSHHLSRLHDLRAAGSGSVLATGLSDDVILEVLAV
                     DPALAVAIDRADTEFHRIAAEFPDLVRLDEAEQARRVQDGYVNFYAADAVNPYVALAA
                     QGPWIVTLKGAVVYDCGGYGMLGLGHAPQAVLDAMNKPHVMANVMTPAVSQMRFIDAL
                     RGEIGATRGGSPFTRFLCLNSGSEAVSVAARIADIATKQMTDPGARYEGCLVRGLTLE
                     GSFHGRTDRPARHSHSTQKNYRKYLASYRSNEYLLTVEPNNIAQLESVFAKAQKDRIF
                     IEAFFMEPVMGEGNPGQGVTPEFYRRARELTEEHGCLFLVDSIQAGLRAHGVLSIVDY
                     PGFRDLPPPDMEAWSKALNAGQYPLSVLGLSGRATTLYQHGVYGNTMTSNPRALDVAV
                     SVLQSITPQVRENIRSAGARLVERLQAAAHGLDGAITKVQGTGLLLSCELDARYKCYG
                     ANSTEEYLRRNGLGVIHGGTNSLRFTPHFRMTEAEGELIVDLTRDALLNGPLRTSE"
BASE COUNT         1330 a         2237 c         2265 g         1406 t
ORIGIN      
        1 cagtgtcctt cgccaggaca aacaattcat cgacaccggc actgaacacg gtcgggccga
       61 gcgaccagtt cagggtctcg ccgttgccgc tccagtgcgg ccagttaccg ccatcgccgc
      121 tggctttggt cattgagacg ctgtcgacgg cggtgctgag ttccaggggt ttgcctccag
      181 gcgggaaggg cagcacgtag acgtgatagt tctcgcgaaa agccaggtgg cgatcgtccg
      241 gcgcgacttc gtacgccgtc acgtatttcc cgcttgcatg ggtcctggcc gctttcccgt
      301 tcaggtcgat gctgacgagc gcagccgcat cgtcgagatc gcgggtgacg tacagccggt
      361 cgccgcgcgc gccgaaatgc ggattggagc cgttgtcggt gacctgtcgc gattcgccac
      421 cgcgaacagg gacggaaaag acgccggtat cgatggacca ttccggtgca gtcagaaacc
      481 cgccttcagt cgcttcgtag acaacggtag cgccatccgg ggaaaagcga ggattggagt
      541 aatgccccgg cttccgtgtc agccgagtgc tgcgccctcc gttcgcgcgt acctgatgaa
      601 cgtgcgaaag ctctgtgtcg ctccaggtga cgtacacgat cgagttcccg tcccgggacc
      661 acgagggaaa atactgaaag tgttccctgc tgtcgcgcgt gagagggcga ggctccccat
      721 cgggaacagg cttgatataa agcctgccgg tgctctcgaa cacgacttgc gagccgtcag
      781 gggaaacacg cgcattgcgc accatccgcg tttcgaaact gtcgggagcg acgtcgatcg
      841 ttggccgggg tggatcgaag accgtacggg tatcgtcgac atcgaatggt atctccgtga
      901 cctcgcggct ttcgcgatcg atacgacgga tttccccccc tgcccagaag acgatgcttt
      961 gcgaatcggg agtccagtcc atgttcgggt acgcgccgtg cacggcccag acctcctgca
     1021 tgtcctggtc gagatcgtcg taaagcggaa tctcggtgcc gctcgtcagg tccttgacga
     1081 acagtgcggt ctttccttcc agcctgcgca cgaaggcgag gtaattgccg tccggagagg
     1141 gtgcagggcg cacggcgccg ccagcccctg tcacgaagct ttcggtcctg ttttcctgca
     1201 ggtcgtgacg gcgaatctcg aagatctggt cgttcgaatc ctgcgcgtag ataaacgtgc
     1261 cgcccggcgt gctgtcgacg ctgaaataaa tgtaacggcc atcgggagaa aaagccggct
     1321 ctcccagttc tttctggtgc tcttcattcg ggcgctcgac cactgcgact cccgagccgc
     1381 cattgcggtg gtacaaccag atctcgccag tgcccagcga gcgggtcgtc gtaaagtgct
     1441 ttcgcgcggc gatgtaatcc ccatccggcg accatgccgg gttgttgaga agccggaaat
     1501 cctctttggt gagctgtcgc ttctcgctgc cgtcggttgg catgatccag atgttgtcgc
     1561 ctccgccggc atcgctcgtg taggcgattt ccgagccgtc gggggaaaag cgcggctgca
     1621 tcgaccatga atgcccgctg tcgatcggcc tggcatcgcc accctctatc ggcacgaggt
     1681 agatgtcgcc gagcaggtcg aacgcgatgg tgcgtccgtc cggactgaca tcgaggctca
     1741 tccacgtgcc gctcgaggtg tggatcggga tctgccgcga atccccgggc ggcgagttga
     1801 cgtcccagcc gttcttgtcc tccttgtcgc tgtcgttttc cgcggcaaga gcgaaagaac
     1861 aaagcagggc gatacagcag gtactgattc cacgggtgag tttcatgtag cgggcatcca
     1921 gtcgtggcga aagggggaat gttatcggac cagcgcaagc cagtcagggt gacgtggatg
     1981 ccggcctggc gaagcggtcg ccgaagaagt ccccctcgtt ccacgccggg cgctcgtcgg
     2041 cgtcggcgac cgccaatcct atccggacgt tggcacgcgc gaagcgcagc gccgagtccc
     2101 agtcgaccgg gcgagagctg tcgtcactcg gccggtggta gtgcgtcgcc aggtgttcct
     2161 ggaacgcggc cgcgccatct acctgtgggt ccgtcgaagt gaagccgggc accaggaaaa
     2221 cggacggcac gccctggcgc acgaacgagt actggtcgct gcgaatgaaa aggacctctt
     2281 caggcacggg atccggggtc aaagcaaagc cttcggcagc gacagcgggt tcgatcagtt
     2341 gctgcagcgt agagtgctca gcgccgaatg cgatgacgtc ggcgagaggg tacaacagca
     2401 gcggcatatc gaggtttacg ttcgcgacca gcgaatccga cggcacggtg gggtagtggg
     2461 cgaagtagtc ggaaccgatg agccctcttt cctcgcccgt cagtgcgatg aacaggatcg
     2521 aacgcggcgg cggctccggc atcgccgcga acgcgcgtgc cgcctcgatc agcagtgaca
     2581 cgcccatggc gttgtcgtag taaccgttgt agatatcgtc gccatcgacc ggctcgccta
     2641 tcccgacgtg atcgagatga gcggaaaaga cgacgtactc gtccttcaac tcagggtcgc
     2701 ttccaggcat gatgccgatg acgttagggc tctcgacctc ttcatggcgc gtcttctggc
     2761 ttagcgttac ctccgtgccg agaggtgtgg agagaggacg gccggcatcg gcggcatcga
     2821 gcgcttcctc gaaagttatc ggcgctccct cgaacaattc tgctgcggtc ggtacgctga
     2881 aggttgcgtc gccttcgatc tcggggtggt agtcagcgac ctcgccggag agattgatcc
     2941 acgacatgcc ctgcagtatt cctgcattca gggtcagtcg ctcccacggt acgcgcttct
     3001 ggtccacgcg cgagcgcagc ccgatgcggc cgatggcacc acgcctgacc atttcctctg
     3061 ccttggttcg tcctgacgaa tagaacgccc gctcgttgtg cgggaatcgc tccggcgcgc
     3121 cgccgaagac ggcgacgatc ttgccgcgca catcgatatc ttcgtagtcc gagtagttca
     3181 tttccggcgc atgcactccg tatccggcga agacgacttc cgcggtgacg ctggtttcac
     3241 ctcgcacttc gtcgccgccc atcacgaagt cctctttcca ctcgagttcc gcgtccccgc
     3301 tgtccctgtg cagggtgact gctgcgctct ctatatccag ccgccggccc agcatcggca
     3361 cgttctggta ccagccttcg tcgcctccgg gtgccaggcc caaggcctcg aactgctgcg
     3421 ccacgtaggc tgcagcgtca tcgtagcctg cagtgccggt catgcggccg gcccgtgcgt
     3481 cgtcggccag gaagctcagg tgcccttgca ggttttcgcg ggtaatttcc gagaggctgc
     3541 tgaagcgatc ttcggtttca acgccggccg gcggccctga gggcgcctct tccgcggggg
     3601 gcgaacaggc tgccatggaa acaatggcaa cgagtgtgat gacaatggga cgaaaagcgc
     3661 atcgaagcat tctgttttcc tgtgaatcgc cgggtttcgg ggagcgggga tcatagcatc
     3721 cgctgcaaag ccagccgcgc acacgcgagt caggctgcaa ttacccttga agtgcgggcg
     3781 atgctaccct ggtgcacccc gatcgctcgc gcgaatgctc tatcatttac gacggggtac
     3841 actagagata tcggcaggct gtccagccca tgccgctgcg gctcttgatc cgccgggcgc
     3901 cttccgcagg cttcccctaa attccacctt atttccagat gtttcgcatt tcgcggaggc
     3961 ccgtgatgac cgagaacacc aaggctactt tgaaggacca ggtcagccag ctagtacgcc
     4021 atatcgaagt ggagagtccc gagctgctcg gtgtcgtgca gagtttccgg cggctggaca
     4081 gggtcgctta caaactcggg ttgctggacc cgaccaggtc gtacgctaca cagattccgt
     4141 ggtggccatt gatctccgtg ctcggcacct attcttccgg gaagtcgacg ttcatcaaca
     4201 atctcctggg tcagccgtta cagaagaccg gcaaccaggc cgttgacgac aagttcacgg
     4261 tgatctgtta cggacgcgag cagacaccgc gcgtattgcc ggggctggcg ctcgacagcg
     4321 acccgcggtt tccgttctac aagataagcc gtgaaatcga gagggtcatg gaaggcgagg
     4381 gagcacgtct cgactcgtac ctgcaactga aaacgtgcaa cagcgaccgg ctgcgcggaa
     4441 agatcgtcat cgactcgccg ggcttcgatg ccgatgcgca gcgcacatcc accttgcgga
     4501 ttaccgacca catcatcgac ctgtcggacc tcgtgctggt gttcttcgat gcgcggcatc
     4561 cggaaccggg cgcgatgcgc gatacgctcc agtacctcgt atcgggaaca ttgcagcggc
     4621 cggacttcaa caagttcatg cacattctca accagatcga caacgctgcg cgcgaggaca
     4681 atcccgagga ggtgtttgcg gcatggcagc gcgcgctggc gcaacagggg ctgacggcag
     4741 gacgtttcta ccggatctac gatcccttcg cctccgtccc gatcgaagac gagcacctga
     4801 agcagcgatt cgagtccaag cgtgacgaag acatggccga aataacgcgg cggatcgaag
     4861 agctggaagt ggagcgctca taccgcgcgg taggcaggct ggagcaactg gccaaacgta
     4921 tacgcgacgt cgtcgtgccc cgcctgacgg ctgcgcgacg tgcctggagg aagcgcgtat
     4981 tgctcctcga cgcgctcgca ttcggcacga tcctcgtttc gttcctggtt ctgagcaaca
     5041 tgctcggctg gtggcagggg ctgcgcttcc agccgccgtg gctggatacc gtagagcgat
     5101 ccccgctcct tttgtggtcc ggcgtcgtaa tagtcctgct cgccgcgtgg cagctacatc
     5161 gcgtattgcg caggttggcg gggcgccgca tcgcaaggca gctgcgccac gatgcggctc
     5221 tgggcgacca ggcgaactcg gtcgccgatg ctttcgacag gaacgtccgc tcgttttggc
     5281 cgttcgtgtt cgccaatcct cgcggctggg gcatatgggc gcggcgcagg ctggacgcga
     5341 tactcaacga cgcctacgcg gccgtacagc agctaaacga ccgatacacg gatccttcgg
     5401 gcacgcggat agcggaagag ggagatgcag atgaagcacc ggatttcgtc ccgcacccgc
     5461 caccgcccgc cggcagaacc ggcgtggaag gtcacgcgcg ctaacagcct tgcgcgggcg
     5521 atgcaaaagg cgttaccgat tttactcaga cgttcgcagc gggccgttca gaagggcgtc
     5581 tctcgtcaga tcgacgatca gttcgccttc ggcttccgtc atccggaagt gcggggtaaa
     5641 acgcaacgag ttcgtaccgc cgtggatgac gccgaggccg ttcctacgca ggtactcctc
     5701 cgtgctgttc gcgccgtagc acttgtagcg cgcgtcgagt tcacaactca gcagcagccc
     5761 cgttccctgg actttcgtta tcgcgccatc gaggccgtgc gccgctgcct gaagccgctc
     5821 gaccagccgg gctcccgcgc tccgtatgtt ttcgcgcacc tggggagtga tgctctgcaa
     5881 cactgacacg gccacgtcga gggcacgcgg attgctggtc atggtattgc cgtaaacacc
     5941 gtgctggtac agcgtggtgg cgcggccgga gaggccgagc accgacagtg gatactggcc
     6001 ggcgttgagt gccttcgacc atgcttccat atcgggaggc ggcaggtcgc gaaagccggg
     6061 atagtcgacg atcgacagga ctccgtgagc acgcaacccc gcctgaatcg agtcgaccag
     6121 gaacaggcaa ccgtgctctt cagtaagttc acgagcgcgg cgatagaact ccggagtcac
     6181 accttgcccc ggattgcctt ctcccatgac cggctccatg aagaacgctt cgatgaaaat
     6241 gcgatccttc tgagccttgg caaaaacgct ttcgagttgt gcgatgttgt tcggctcgac
     6301 cgtcagcaaa tattcattgg aacggtacga cgccaggtac ttgcggtagt tcttttgcgt
     6361 cgaatgcgaa tggcgcgcag gacggtcggt gcgcccatgg aaacttcctt ccagcgtcaa
     6421 cccgcgcacg aggcaaccct catagcgtgc gccggggtcg gtcatctgct tggtggcgat
     6481 gtccgcgatg cgcgccgcca ccgagacggc ctcggagccg ctgttcaggc acaaaaagcg
     6541 cgtgaagggt gatccaccgc gagttgcccc gatttcgccg cgcaacgcgt caatgaatct
     6601 catctggctg accgccggtg tcatgacatt ggccattacg tgcggcttgt tcatcgcgtc
     6661 gagcactgcc tgcggcgcat gtccaagccc gagcatgccg tagccgccgc agtcgtagac
     6721 gaccgcaccc ttcagggtaa caatccaggg gccctgggct gcgagagcga cgtatggatt
     6781 gacggcgtcc gcagcgtaga aatttacgta tccgtcctgg acgcgccgcg cctgctccgc
     6841 ctcgtccagg cgcaccaggt ccgggaattc cgcggctatc cggtggaatt cggtatcggc
     6901 acggtcgatc gcgaccgcaa gggccgggtc gaccgccagg acttcgagga tgacatcgtc
     6961 ggacaatccc gtcgccagca cgcttccgct gccggcggcg cgcaggtcgt gcaaacggga
     7021 caggtgatgg ctggtatcga cgatattggc tgggcggttc atgtttcgat tcctcgcgtg
     7081 aaccggacat tgtatagact tgcgcggtgt aattcgcagg ccgccgcacc atatggcccc
     7141 gggtgcagcc ggaaaagcgg cgctgcccgg tacagccaat gctccggcgc gcctcgcttt
     7201 cccccttttt gcccgtacaa tcccggccca tgctcaag
//
LOCUS       DASUOM010000194         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1336589,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000194 DASUOM010000000
VERSION     DASUOM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336589"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..3388
                     /locus_tag="VFY27_04030"
     CDS             35..3388
                     /locus_tag="VFY27_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEX5764710.1"
                     /translation="MAICLSMALSACGGKAFLAETPESASFLERRMTQQEGPVTVAVA
                     VPDAAETEALTGLPLYDQGIQPVWVEVTNDGPGQVRLSVASIDPEYYSPHEVAWMNRR
                     GYSDEARAGMERWFYTNAIRRHVPPGESRSGFVFTRLAAGTKGFNVDVISQELTSYNF
                     TFFVKMPGFTADYMDVDFKNLYEQDEFIEIAAEELPDTIRRERCCGTDETGTMQGDPF
                     NAVIVSTPLALRRALLRAGWQETAIDSPDTAVARMQHYRGRRPDGTFIKIRPDGTERK
                     EARIWYTPFVSDGEPVWAVQVLYNLSSSGRAAGEQRVDPDLDEARNFFLQDLWYSQSL
                     RQGTFIKAYDAVPAEKPAATFTGSTYFTTGYRAVAWLSEEPVAMDNTVLLNWGQFGDA
                     LRGAAQASPSSMTEPQTYPPPNQRLHEQQFGSVRVSTAVPSAAESQAIFDVNLYRQNV
                     QPVWVQIENLSDKGMYFLPVGLDPAYYTPIETASRYFPWDRILSRLAGEDFRRRNIQM
                     LVDAGSSRSGYVFTRVDEGTKSFNVDVFPVGGNPYQMSFFVPVPGLRVDHYDVEFESL
                     YSESDVRNVDLEGLVAGLEALPCCAADNKGRLVGDPLNIAFVGEREDLYYSLLRAGWD
                     ETETIYGKSAVKTGASALFGTEYRYSPVSALYAFGRAQDAAFQRARSTINERNHMRIW
                     LTPMRFENKPVWIGQISRDIGVRFTWKTITTHKIDPDVDETREFLIEDLAYAQGLAAI
                     GHVVGVGAAPFEQPRRNLTGDPYFTDGLRALMWLSASPVSISEIEIRDLGESPSDRRR
                     SAVPAHVDARARFRGIYCAVTEANGRNLPDYRPCEDALWPLDQASSPAREPVLLEPLR
                     SDLHYLLVPGLGWDCIAGLVEQPGYSAVEHARALGLTLEMIEVDGLAGTEHNAAIIRD
                     HVLAQGRAAERNLVLIGYSKGAPDILEGLVRYPEIRQYVRAVVSVAGAVGGSPLADDA
                     SDWVLDLLQLVPGSDCKETDGTAIDSLRPVTRKLWFDQHELPDDIRYYSIVSYTDAEH
                     VSPILKRSYNKLARIDTRNDSQVVYFDQIIPGSELLAYLNADHWAVAVPIERSKRGPV
                     VDGALNNAYPREVLFEALARYLDQTLNFE"
     gene            3929..4393
                     /locus_tag="VFY27_04035"
     CDS             3929..4393
                     /locus_tag="VFY27_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764711.1"
                     /translation="MTNNDSSYLLPASAQPPSNKQRLFIRYFTAILIDLVVLNLFVEY
                     SEYVVIDSFTISLFAALLLQVLLKLTLAIEHRVAAYFKARQGGFAKFMRYFSAWLLIF
                     GSKFVILEAIAIAFGDKVYFGGPLHGLVVLIVAVITMLVAEEAIVQLYRRLA"
     gene            4410..>5124
                     /locus_tag="VFY27_04040"
     CDS             4410..>5124
                     /locus_tag="VFY27_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764712.1"
                     /translation="MSTNDATLRKFLSKDLTETLIRVGLIAFLVVMCIRVFAPFAGLV
                     MWGLILAIALEPLHGRLAARLGGRQGLAATVMMLGGLLLLGVPTVMLGSSFASHLQDV
                     YAAVESGTLAIRPPDSGVAEWPIVGARVYEAWNSAATNLPAFVKENQELLRNLSRQVL
                     AAAASTAGAILLFLGAMIVAAIMMAYTESGSQTIRRIFCRLTDPVRGPGLQKLATATV
                     RSVAAGVIGVAFIQSILLGM"
BASE COUNT         1009 a         1535 c         1585 g          995 t
ORIGIN      
        1 gtcgttgcgt tcaggcaaat tctctggact cctggtggcc atatgcttgt ccatggcgct
       61 gtccgcctgc ggcggtaagg catttcttgc cgaaacgccg gaaagtgcga gctttctcga
      121 acgtcgaatg acccagcagg agggcccggt gacggttgcg gtcgcggtgc cggacgctgc
      181 cgagacggag gctcttaccg gactcccgct ctatgaccag ggcatacagc ccgtctgggt
      241 cgaagtcacg aacgacggtc cgggccaggt gcggctgtcc gttgccagca tcgaccccga
      301 atactactcg ccgcatgaag tggcctggat gaatcgacga ggctacagcg acgaggccag
      361 ggccggcatg gagcggtggt tctacacgaa tgccatcaga cggcatgttc cgccgggcga
      421 gtcccggtcc ggtttcgtat tcacacgcct ggcggccgga acgaagggat tcaatgtcga
      481 cgtgatctca caggagctga cctcctacaa cttcaccttt ttcgtgaaga tgccgggctt
      541 cacagccgac tacatggacg tggatttcaa gaacctgtac gaacaggacg aattcatcga
      601 gatagcagcc gaggaactgc ccgacacgat tcgccgggag cgctgctgtg gcaccgacga
      661 aaccggcaca atgcaaggtg acccgttcaa cgccgtcatc gtgtccacac cgctcgcgtt
      721 gcgccgtgcg ttgctgcgcg ctggctggca ggagaccgcg attgacagtc cggatacggc
      781 cgtcgcacgt atgcagcact atcgtggccg gcggccggac ggaacgttca tcaagatccg
      841 gcctgacggc accgagcgca aggaggcccg gatctggtac acgcccttcg tgtcggacgg
      901 cgaacccgtg tgggccgtcc aggtgctgta caacctcagc agctctgggc gggcagccgg
      961 tgaacagcgc gtggatcccg atctcgacga agcgcggaat tttttcctgc aggacctctg
     1021 gtacagccag tcgttgcggc agggcacgtt catcaaggcc tatgacgcag tacctgccga
     1081 gaaaccggcg gcgaccttca cgggctctac ctattttacg accggttatc gcgcggttgc
     1141 ctggctgtcc gaggaaccgg tggcgatgga caacaccgta ctcctcaact ggggacagtt
     1201 tggggacgcg ctccggggag cagcgcaagc gtcgccgagt tcgatgaccg agccgcaaac
     1261 gtatcctccg ccgaaccaac gcctgcacga acagcagttt ggcagcgtac gcgtatcgac
     1321 agcggtcccg tcggccgcgg aatcacaggc gattttcgat gtgaacctgt acaggcaaaa
     1381 cgtccagccg gtatgggtgc agatcgagaa cctgagtgat aaaggcatgt acttcctgcc
     1441 ggtggggctc gacccggcgt actacacccc catcgagacg gcttcacgct acttcccgtg
     1501 ggatcgcatt ctgagtcgac tggccggcga agacttccgc cgcaggaata tccaaatgct
     1561 ggtcgatgcc ggcagctcgc gctccggtta tgtattcacg cgagtggacg agggaacaaa
     1621 gtccttcaac gtcgacgtct tccctgtcgg gggcaacccg taccagatga gttttttcgt
     1681 gcccgtgccc ggattgagag tcgatcacta cgatgttgag ttcgaaagcc tgtactctga
     1741 aagcgatgtc cggaacgtgg acctggaggg actcgttgca ggcctcgagg cgctgccctg
     1801 ctgcgccgct gacaacaagg gccgacttgt cggcgatccg ctcaacatcg cttttgtcgg
     1861 tgagcgcgag gacctctatt acagcctgtt gcgtgccggc tgggacgaaa ccgagacgat
     1921 ctatgggaaa tccgcggtaa agaccggtgc ctcggctctg ttcggcacgg aatatcgata
     1981 ctccccggtc agtgcactgt acgcgttcgg acgggcgcag gatgcggcgt tccagcgagc
     2041 ccgctccacg ataaatgaac gcaatcacat gcgcatctgg ctgacgccca tgcgcttcga
     2101 aaacaagccc gtctggattg gccagataag ccgggacatc ggcgtccgct tcacatggaa
     2161 gacgattaca acgcacaaga tcgaccccga cgtcgacgaa acgcgtgagt tcctgatcga
     2221 ggatcttgcc tatgcgcagg gccttgccgc catcggccac gtggttggcg taggcgcagc
     2281 gcccttcgag cagccgcgca ggaacctgac cggggatccg tatttcacgg acggacttcg
     2341 tgccctgatg tggttgtctg cttctcccgt cagcatcagt gagatcgaga tcagggacct
     2401 cggtgagtcg ccgagcgaca ggcggcgctc cgcggtaccg gcgcacgtgg acgcgcgcgc
     2461 ccgtttccgg ggaatctatt gcgccgtcac cgaggcgaac ggccgcaacc tgccggatta
     2521 ccggccttgc gaggacgccc tctggcctct cgaccaggcg tcaagcccgg cccgggaacc
     2581 cgtactgctc gagccgctcc gctccgacct ccactacctc ctcgtcccgg ggctcggctg
     2641 ggattgcatt gccggactgg tggagcagcc gggctattcg gcggtggaac atgcacgagc
     2701 gctggggctg acgctggaaa tgatcgaggt agacggcctc gccggtacgg aacacaacgc
     2761 cgcaataatc cgggatcacg tgctggcaca gggcagggcg gccgaacgca atctcgtgct
     2821 tatcggctat tccaagggtg ccccggatat cctggagggc ctggttcgtt atcccgaaat
     2881 caggcaatac gtgcgggcag tggtcagcgt cgccggcgcc gtcggcggat cgcccctggc
     2941 ggatgatgcg tcggactggg tcctcgatct gctgcaactg gtcccgggat cggattgcaa
     3001 ggagacggat ggtacggcca tcgactcgct gcggccggtc acgagaaagc tctggttcga
     3061 ccagcatgag ctgccggacg acatccgcta ctactcgata gtcagctaca ccgatgcgga
     3121 acacgtttcc ccgatcctga agcgaagcta taacaagctc gccaggatcg atacgcgcaa
     3181 cgacagccag gtggtgtatt tcgaccagat cattccgggc agcgagttgc tggcatacct
     3241 caatgccgac cactgggccg ttgccgtgcc gatcgagcgc agcaagcgag ggccggtcgt
     3301 cgatggcgcg ttgaacaacg cgtatccacg tgaagtgcta ttcgaagcgt tggcacgtta
     3361 cctcgaccaa acgctgaact tcgaataggc accgactcgc ggatgtgctg gggtctcccc
     3421 gaaacagtgg atgaaccagc aagcgaccta cgacctggat aaggcagcgc gcgcgaggcg
     3481 cgtagcctga ttacacagga ttcgatggac cgtggtcggc ggccaacatg atcgcgcgag
     3541 tgtctgcgcc tgcgttcctc tcggttgcgc aaatttgata gtaagcaacc caacgaccag
     3601 atattgttcc cgttcggtgg aaatgtccgc cgcgcttcag cgcggagacc ggtcgcgacg
     3661 cccgccgtag cggacgcgga aggtctgcgg aaaaaatgcg cttgcattgc ttgctaccag
     3721 gcaaacgcca aacggcagga cagcttacaa ggacgtcgcg gcaagatatc gcggctcgga
     3781 aggtgccggg cagatgctct tcgacaaagt caagaatggc ggcgtgggag tttggggcca
     3841 gataccgatg cccccaacgc caccgtgccg gacgcagaac ttcaagcgat gatcacgttg
     3901 attctcgccg tgaactgagt gtcaaaaaat gacaaacaac gatagcagct accttctccc
     3961 agcctccgcc cagccacctt cgaacaagca aaggctgttc atacggtatt tcactgcgat
     4021 cctgattgat ctcgtcgtgt tgaacctgtt tgtcgagtat tccgagtacg tcgtcatcga
     4081 ctcgttcact atctccctgt tcgccgcact gttgttgcag gtgctgttga aactgacctt
     4141 ggccatagag caccgggtcg ccgcttattt caaagcccgg caaggcggct ttgcaaaatt
     4201 catgcgctac ttcagcgcgt ggctactgat tttcggatcc aagttcgtga ttctcgaggc
     4261 cattgctatc gcgttcggtg acaaggtcta tttcggcgga cctctgcacg ggctggtagt
     4321 cctgattgtg gccgttatca cgatgttggt tgccgaggag gcaatcgtac agctttatcg
     4381 ccggcttgcc tgacgagagg agtacggcaa tgtcgacaaa cgacgcgaca cttcgcaaat
     4441 ttttgtccaa ggacctcacg gaaaccctga tacgcgtcgg cttgatcgcc ttcctcgtcg
     4501 tcatgtgcat acgtgtgttt gcaccgttcg cgggcctggt gatgtgggga ctgattctgg
     4561 cgatagcgct cgagcccctg cacggcaggc tggctgcacg cctgggcggc aggcagggtc
     4621 ttgctgcaac ggtgatgatg ctcggcggcc tgctgctgct cggggtgccg actgtcatgc
     4681 tcggaagctc gttcgcgagc catttgcaag atgtctacgc ggccgtcgag agcggtacgc
     4741 tggcaatccg gccgcccgac tcaggcgtgg cggaatggcc gattgtcggt gcgagggtat
     4801 acgaagcgtg gaactcggct gccaccaatt tgccggcttt tgtcaaggag aaccaggaac
     4861 tgctcagaaa cctgtcgcgg caagtcctgg ccgctgcggc cagcacagcg ggggcaatcc
     4921 tgctgttcct gggcgcgatg atcgtcgccg cgatcatgat ggcctacacc gaatccggca
     4981 gtcaaacgat ccgccgcata ttctgccggc tgaccgaccc ggtcagagga ccaggactgc
     5041 agaagctcgc caccgcaacg gtacgctccg tcgcagcagg cgtgatcggc gtggccttta
     5101 tccagtccat cctgctcggc atgg
//
LOCUS       DASUOM010000195         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_637091,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000195 DASUOM010000000
VERSION     DASUOM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637091"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /gene="pnp"
                     /locus_tag="VFY27_04045"
     CDS             complement(<1..1884)
                     /gene="pnp"
                     /locus_tag="VFY27_04045"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5764713.1"
                     /translation="MKAIKKTFQYGEHQVTIETGAVARQADGAVLVDMAETVVLVTAV
                     GRKTAEPGRDFFPLTVNYQERTYSAGKIPGGFFKREGRPSEKETLVSRLIDRPLRPLF
                     PKGFTNEVQVIATVVSLNPDVDPDIPALLGASAALAISGMPFAGPIGAAKVGYRDGSY
                     ILNPGVEALAESKLELVVAGTADAVLMVESEAKGLPEDVMLGAVMFGHEQMQQAIKVI
                     NELAAEAGKPAWEWQAAVADSELESLVTRAAETALADAYRVTDKQDRQAKVADAKNAA
                     IESLPGGGDARWSEDEIKTALSKLEKRIVRNRVIAGEPRIDGRDQKTVRPIHIEVGVL
                     PRAHGSALFTRGETQALVVTTLGTGRDAQIIDAIEGERKEPFMLHYNFPPYCVGETGF
                     VGSPKRREIGHGKLARRGIQAVMPKSDEFPYVIRVVSEITESNGSSSMASVCGTSLAL
                     MDAGVPTKAPVAGVAMGLVKEGENFAVLTDILGDEDHLGDMDFKVAGTSDGITALQMD
                     IKIHGITREIMSRALEQAKEARLHILSEMNAIIAAPREQMSEWAPTIITFKIDPEKIR
                     DVIGKGGAVIRAMTEETGASIDIENDGTVRIASVDGASGREARRRIELITADVEVGRV
                     YEGK"
     gene            complement(2031..2300)
                     /gene="rpsO"
                     /locus_tag="VFY27_04050"
     CDS             complement(2031..2300)
                     /gene="rpsO"
                     /locus_tag="VFY27_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5764714.1"
                     /translation="MSLSSEAKSSIIEDYSRGDADTGSPEVQVALLSARISELTGHFG
                     KHVKDHHSRQGLLKMVNKRRKLLDYLKSRDPDRYRELIDRLGLRR"
     gene            complement(2440..3378)
                     /gene="truB"
                     /locus_tag="VFY27_04055"
     CDS             complement(2440..3378)
                     /gene="truB"
                     /locus_tag="VFY27_04055"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5764715.1"
                     /translation="MTAAAPERRRPTAVTGLLLLNKPAGLTSNQALQKVKRLLNAKKA
                     GHTGSLDPAATGMLPLCFGEATKVCAYLLEADKSYRVTARLGEATDTGDADGVIIQTE
                     AVPQLSAADWEQVLARFTGEIEQVPPMYSALKKDGKRLYELARQGQVVEREARKIRIH
                     ELQLLEAHGSRLVFRVRCSKGTYVRTLVEDLAKAAGTVAHTAALHREAVGEFAAADML
                     DMPSAERIAAAGPEALREHLIPADRALQQWPEKRIGPDAARRFNDGQSIIVGADDGNC
                     EAGHVRVYAADSGFLGVGELTGDGMLTPRRIFRAGR"
     gene            complement(3375..>3681)
                     /gene="rbfA"
                     /locus_tag="VFY27_04060"
     CDS             complement(3375..>3681)
                     /gene="rbfA"
                     /locus_tag="VFY27_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5764716.1"
                     /translation="LLRFETKDPSLATVSLTAVDLSRDLGVAKVYYSLLNPDDDPQPV
                     QEGLQRASGFLRGRLGRSLEIRHVPELRFVHDDSIAHGVEMNRLIDSATKSGEGNPE"
BASE COUNT          652 a         1212 c         1057 g          760 t
ORIGIN      
        1 cttgccttcg tagacgcgcc ctacttccac gtcggcggtg atcagctcga ttcggcggcg
       61 tgcctcccgg cctgatgccc cgtccacaga ggcgatacgg accgtgccgt cgttctcgat
      121 gtcgatcgac gcccccgttt cttcggtcat ggcgcgaatg actgccccgc ccttgccgat
      181 aacgtcgcga atcttctccg gatcgatctt gaacgtgatg atcgtcggag cccattcgga
      241 catctgttca cgcggcgcgg caatgatagc gttcatctct gagagaatat gcaggcgggc
      301 ttcctttgcc tgctccagag cgcgactcat tatttcccgg gtgatgccgt ggatcttgat
      361 gtccatctgc aacgcggtga ttccgtcact cgtacccgca accttgaagt ccatgtcgcc
      421 gaggtgatct tcatccccga ggatgtccgt gaggacagcg aagttctcgc cctccttgac
      481 cagtcccatg gcaaccccgg caaccggtgc cttcgtcgga actccggcgt ccatcaatgc
      541 caggctggtg ccacacacgg aagccatgga actcgagcca ttggattcgg tgatttcgga
      601 gacaacgcgg atcacgtaag gaaactcgtc ggatttcggc atgacagcct ggatgccgcg
      661 acgcgcgagc ttgccgtggc cgatctcgcg ccgcttcggc gagcctacaa agcccgtttc
      721 gccaacgcag tacgggggaa agttgtagtg caacataaac ggctctttgc gctcgccttc
      781 gatcgcatcg atgatctgcg catcccgtcc cgtgcccagt gtcgtcacga cgagagcctg
      841 tgtttcacct cgtgtaaaca gggcagagcc gtgcgcccgc ggcagaacgc cgacttcgat
      901 gtgaatcggc cgtactgtct tctggtcgcg gccgtcgata cggggttcgc ccgctataac
      961 gcgattgcga acgatgcgct tttcgagctt ggaaagagct gtcttgatct cgtcctcgct
     1021 ccaccgtgca tcgccgccgc cgggcaacga ttcgatggcg gcgttcttgg cgtcggcgac
     1081 cttcgcctgg cgatcctgct tgtcggtaac ccggtaggca tcggcaagtg cggtttctgc
     1141 agccctggta acgagcgact ccagctccga gtccgcaacc gctgcctgcc attcccacgc
     1201 cggcttaccg gcttcggctg ccagctcgtt gatgactttg atcgcctgct gcatctgctc
     1261 gtggccaaac atgaccgcgc ccagcatgac gtcttcgggg aggccttttg cctccgactc
     1321 caccatcagc actgcgtccg cagtacccgc gacgaccagt tcgagcttcg attctgcgag
     1381 tgcttcgaca ccagggttca ggatgtagct gccgtccctg taaccgacct ttgcagcacc
     1441 gatcgggccc gcaaacggca tgccggaaat ggcaagcgct gcggaagcac cgaggagtgc
     1501 cggaatatcc gggtccacat cggggttcag cgagacgacc gtagcgatga cctgcacttc
     1561 gttggtgaag cctttcggga agagcgggcg caacggacgg tcgatcaggc gcgacaccag
     1621 cgtctctttc tcgctcgggc gcccctcccg cttgaagaaa ccgcccggaa tcttgccggc
     1681 agagtaggtt cgctcctggt aattgaccgt cagcgggaaa aaatcccgtc cgggctccgc
     1741 cgtcttcctg ccgactgcgg taacgaggac aacagtctcc gccatgtcga cgagaacagc
     1801 gccatccgcc tggcgcgcaa ccgcgcccgt ttcgatagtt acctggtgtt cgccgtactg
     1861 gaacgtcttt ttgatagctt tcacgtgatt actcggaata agtataagaa tggcgccgaa
     1921 ggttggcagc accgcgatcg cggttgatat aactgccggg cagccgacga tgaagcccgt
     1981 acacgggccg atccgtcggt tcaatcatag ccagtggcgc cgctgtcttg ttagcggcgg
     2041 agaccgagcc gatcaatcag ctcgcggtac cggtccgggt cccgggactt caggtagtca
     2101 agcaacttcc gccgcttgtt caccattttc aaaaggcctt gccgggaatg atgatccttt
     2161 acatgcttgc caaaatgccc ggtaagctcg gagatgcgtg cagacagcaa cgcaacctga
     2221 acttcaggcg acccggtatc agcatctcct cgcgaataat cctcaatgat gctgctcttc
     2281 gcttcactcg acagtgacat cttcaactac tccactcttt ctcttcaccc ggccttttgg
     2341 cagcgcgcta ttctagccgc agaaccggtc cagtaacagc caaaaaggcg taaaaatcag
     2401 ggattgcgca ggccataccg cgttcgcggc cagtccgggt catctccctg ccctgaaaat
     2461 ccggcgcggt gtgagcattc cgtccccggt cagctcaccc acgcccaaaa acccgctatc
     2521 agctgcgtac acccgcacgt gccctgcttc gcaattcccg tcgtcggcgc ctacgataat
     2581 ggactggccg tcattaaagc gccgcgccgc atccggcccg atacgcttct cgggccattg
     2641 ctgcaaggcc cggtctgcag gtatcaggtg ctcgcgcagg gcttccggcc ccgctgcggc
     2701 aatcctttcg gcggacggca tgtccagcat gtccgccgcc gcaaattcgc ccacagcctc
     2761 ccggtgcagc gcggcagtgt gcgccacggt gcccgctgct ttcgccaggt cctcgaccag
     2821 tgtcctgaca taggtgccct tggagcagcg gacccggaaa accagccggc tgccgtgagc
     2881 ttccaggagc tgtagctcgt gaatgcgaat cttgcgggct tcccgctcca cgacctggcc
     2941 ctgccgcgca agctcgtaca atcgcttgcc atccttcttc aacgccgagt acatcggcgg
     3001 cacctgctcg atctcgccgg taaagcgcgc caacacctgt tcccaatcgg cggccgagag
     3061 ttgcggtact gcctcggtct ggatgatgac gccatcggcg tctccggtat ccgtcgcctc
     3121 tccaaggcgc gcggtaaccc ggtaggactt gtcggcttcg agcaggtagg cgcaaacctt
     3181 cgtcgcctcg ccgaaacaga gcggcagcat tccggtcgca gcgggatcca ggctcccggt
     3241 gtgccctgcc ttcttcgcgt tcagcaggcg cttcaccttc tgcagcgcct ggttcgacgt
     3301 caggccggcc ggtttgttca gcaataacag tcctgtcacg gccgtcgggc ggcggcgctc
     3361 gggcgctgcc gccgtcattc cggattgcct tcgccggact tcgtggcgct gtcgatcagc
     3421 ctgttcatct cgacaccgtg ggcgatgctg tcatcgtgca cgaaacgaag ttccggcacg
     3481 tggcgtatct ccagcgatct gccgagcctg ccgcgaagga agccggaggc tcgctgcagc
     3541 ccttcctgca ccggctgcgg atcgtcgtcg gggttgagaa ggctgtaata gaccttcgcc
     3601 acgccgaggt cgcgcgacag gtccaccgcc gtcagcgaga cagtcgccaa actcgggtcc
     3661 ttggtttcga atcgcagcag t
//
LOCUS       DASUOM010000196         4910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1221563,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000196 DASUOM010000000
VERSION     DASUOM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221563"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VFY27_04065"
     CDS             complement(<1..1985)
                     /locus_tag="VFY27_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764717.1"
                     /translation="MTTIALSSGSIGISTVGEARKLEIRLLGELAILADGVSRALPPS
                     RKTRGLLAYLALADRPRRREDLCELLWDAPGDPRAALRWSLAKLRPLLSHGRASALLT
                     DRDTVALDFDLLSVDVLEARSILSPAAGRTDERALEQLADRFSAGYLNGLEDLGTRQF
                     QLWLEAENTSLRDLHRSIVTELLSKPRLPVAALLKLAGKLVALDPLSNASNIRYLKLV
                     FETAGLSEARQAFDRIRKHYGDERQDDAVLRAGWHSIVRADPGSNTAPLAEQDDLSPQ
                     KVGNAFPEKPSVAVLDFQDIGSHAHGAVLANGLTVDLNSRLAQLPSLFVIARQSTARI
                     VALDLAPRQIGAKLGARYLVSGSTQRDNKRVRTTITLTDTAEQRELWSEHFDRPLDDL
                     FQVQDDITNAVIAAIEPAIERAEMQRALLKPPGNLTAWECFHRGLWHCFRFTAKDNEI
                     AHRLFVEAVTLDPHFSRAFAGLSFTHYSRAFLNTVPDVDREIRKAVDAASNSVDFDGR
                     DAMGHWALGRALFLSRQHDRALSAIDRALLVNPNYAQGHYARGFVGIHAGLDDASLPS
                     LEMAQRLSPFDPLLFAMKSSRAISLANRGQPGEAASWAIAATQEPNAHFHIYAVAAAC
                     LEMAGRSADARNNAGWVLERQPKYSVDVFRRSFPHKD"
     gene            2141..2356
                     /locus_tag="VFY27_04070"
     CDS             2141..2356
                     /locus_tag="VFY27_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX5764718.1"
                     /translation="MPHIQVHAIRDVFTAEQKREVIEKLTDAMVSVEGENLRGVTWVS
                     FHEVESGNWGIGGQCLTTADVKALQKG"
     gene            2525..4741
                     /locus_tag="VFY27_04075"
     CDS             2525..4741
                     /locus_tag="VFY27_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764719.1"
                     /translation="MRNFVSELRRRNVLRVAAAYALSAWIIIEAGSVLLPTFGASEKF
                     FQGYVIVVIAGFVVSVILAWIYQVTPEGVKRDRDANHSTPPDPRSRQRMNYSIIALLV
                     VALIVSVTFNVSNIPDAGSGETWKTSVAVLPFPSRSDNPENRIFADGIHDDLLTRLAN
                     IRELKVISRTSVMEYRDTTKNVRVIGEELGVDSVLQGTVQRIGDSVRINLRLIDADTD
                     EHIWAETYDHQLTMENIFAVQSQISESVADALQARLTAGERVRVASVPTSDLRAFRLY
                     KEARSNLYQRRLETLRLARQQFAEAIALDPAFAEAHAGLAESFMLLSINHQDIPEGEA
                     ARLATSSVETALELDPNLADAYAVRGLIKMNVWSRTRIGPENIEAEEAFRRAIALNPS
                     HASAYMWFASLRDSEDRLEDAIELYQRSMELDPLARIPYSNLPMVYAKRGEHKAAMRL
                     WLQATRIHSEWPTVYQYIAVQLWGLGRFDEAYAWYAKAMELGAAPGISGNADVGILVD
                     LGDLEQAEAVLGRFDESHSFFPFAEGIRQLIRGDYEHAATTFSTILDNRLLPERYVLE
                     TAANTALLAGDLESAKRYTLAASPTLAGDAAGKVDRSSANNAIKLAFIYQHEGRDAEA
                     SLYLSELLDLIHDAPRLGTFGYGIRDVQIYALLGRREDAIAAFREALDEGYRGSVVFD
                     GWPLSIDPFLDNIRDDPRFIEMVTELDSHLAVMRDRLYAAQLSDGLDELRARAGTT"
BASE COUNT          974 a         1474 c         1537 g          925 t
ORIGIN      
        1 tcgtccttgt gtggaaagct gcgccggaaa acatcgaccg agtatttagg ttgccgttcg
       61 agtacccagc cggcgttatt ccgtgcatcc gccgagcgcc ccgccatttc caggcacgcg
      121 gcggcgacgg catagatgtg aaagtgcgca ttcggctcct gcgtcgcggc gatggcccag
      181 gacgcggctt ctccgggctg gccccggttg gccagcgaga tcgcccggct ggatttcatg
      241 gcgaacagga gcgggtcgaa gggactcagt cgctgcgcca tctccagcga tggcagcgac
      301 gcgtcatcca gtcctgcgtg tatgccgacg aagccgcgag cgtaatgccc ctgcgcgtaa
      361 ttgggattca ccagcagggc gcggtcgatc gccgacagtg ctcggtcgtg ttgccgggag
      421 agaaacagcg cgcgccccag tgcccagtgg cccatggcgt cacgcccgtc gaaatcgacg
      481 ctgttgctgg cggcgtcgac cgccttccga atttcacggt cgacatccgg tacggtgttg
      541 aggaacgccc tcgagtaatg cgtgaaggaa aggcccgcga aagcacgtga gaagtgtggg
      601 tccagggtca cggcttcgac gaacagccgg tgggcgatct cgttatcctt cgcggtgaag
      661 cgaaagcaat gccacaggcc gcggtgaaaa cattcccatg ccgtcaggtt cccgggcggc
      721 ttgagcagcg cccgttgcat ctcggcgcgc tcgatcgccg gctcgattgc cgcgatgacg
      781 gcgttcgtga tgtcgtcctg tacctggaag agatcgtcca gcgggcgatc gaagtgctcc
      841 gaccagagtt ccctttgctc ggccgtgtcg gtcagagtga tcgttgtccg cacacgcttg
      901 ttgtcgcgtt gcgtcgagcc gctgaccagg tatcgggcgc ccagttttgc accgatctgc
      961 cgcggcgcga gatccaaggc tactatacgc gccgtcgact gtcgcgcgat cacgaacagg
     1021 ctgggaagtt gagccaacct tgagttcagg tcgacggtca ggccgttcgc cagtaccgcc
     1081 ccgtgagcgt gagaaccgat atcctggaag tcgagcactg caaccgaagg cttttcaggg
     1141 aatgcattcc cgaccttctg cggggacaga tcgtcttgtt ctgccagcgg tgccgtattg
     1201 ctgcccggat ccgcccgcac gatcgaatgc cagccagcgc gcagcacagc atcgtcctgc
     1261 cgttcgtcgc catagtgctt gcgaatccgg tcgaatgcct gccgcgcttc gctgaggccg
     1321 gccgtttcga acaccagctt caggtagcgg atgttggagg cgttgctcaa aggatcgagc
     1381 gccaccagtt tgccggcaag ttttaacaat gctgccacgg gcagccgtgg tttcgacagc
     1441 agctcggtaa cgatcgagcg gtgcaaatct cgcagcgacg tattttccgc ttccagccac
     1501 agctggaact gccgggtgcc aagatcctcc aggccgttca ggtatccggc agaaaacctg
     1561 tccgcgagct gttcgagtgc tcgctcgtcc gttcgccctg ccgcaggcga aaggatcgac
     1621 cgcgcctcga gtacgtcaac ggacaggaga tcgaagtcca gcgccacggt gtcgcgatcc
     1681 gtcagcagag ctgatgcccg gccgtggctc agcaacgggc gcaacttcgc aaggctccaa
     1741 cggagcgccg cgcgcgggtc tcccggggcg tcccacagaa gttcgcaaag gtcttcccgc
     1801 cgccggggcc tgtcggccag cgccaggtag gcgagcaggc cgcgggtttt tcgcgacggg
     1861 ggcaatgcac gggagacgcc gtctgcgagg atggcaagct cgcccagcag ccgtatttcc
     1921 agttttcggg cctcgcccac agtgcttatc ccgatgctgc cgctagagag cgcgatcgtc
     1981 gtcatgcaac gcccttttca acgggaatgc caacgccggc tcaatcgcag ggtagtcaac
     2041 atagctgtcc tggcagtcat gctgccctcc gccgaaatcc ttcggcgcag ccatcaaagt
     2101 tctgtttctc tagtggaagc agaaggaagg agtaagagca atgccccaca tacaggtcca
     2161 tgctatcaga gatgttttta ccgcggaaca gaagcgcgaa gtgatcgaaa agctcaccga
     2221 cgcgatggta agtgtagaag gtgaaaacct gcgcggcgtc acctgggtgt cgtttcacga
     2281 ggtggagagc ggcaactggg ggatcggcgg ccagtgcctg acgaccgctg acgtaaaggc
     2341 cctgcagaag ggctgaaggg acggtacggt tggattacca gggagcctct gcacaagtct
     2401 ggacggaaag acggcatcca tgacttgtgc agaggttccc tggtccggac tgatccggat
     2461 tggtgatccg gaacctccgc ggaaacggca ttgcggcgcc ggatcgtcgt ttatactccg
     2521 cggcatgcgc aattttgttt ccgaactcag gcggcgcaac gtgctgcgcg tcgcggctgc
     2581 gtacgcgctt tctgcctgga tcatcatcga agccggttcc gtgctgcttc cgaccttcgg
     2641 cgcttcggag aaatttttcc agggttacgt aatcgtcgta atcgccgggt tcgtcgtctc
     2701 agtgatcctg gcgtggatat accaggtcac gccggaggga gttaagcgcg accgcgatgc
     2761 aaatcactcg actccgccag acccgcgatc ccggcaaaga atgaattact cgatcatcgc
     2821 gctgctcgtg gttgcgctga tcgtttcagt aactttcaac gtgtcgaata tccccgacgc
     2881 cgggagcggc gaaacctgga aaacctccgt tgcggtgctg ccgtttccca gccgcagcga
     2941 caatcccgag aatcgcatct tcgccgacgg cattcatgat gatctcctca cgcgtctggc
     3001 gaacatccgg gagctcaagg tgatttcccg cacctccgtc atggagtatc gggataccac
     3061 gaagaacgtg agggtgattg gtgaagaact gggggtcgac tccgtgcttc aaggcacggt
     3121 gcagcgcatt ggggacagcg tgcgcatcaa cctgcgactc attgacgctg ataccgatga
     3181 acacatctgg gccgagacct acgaccatca gctgacgatg gaaaacatct tcgcggtaca
     3241 aagccagata tcagagtcgg ttgccgacgc gttgcaggcg aggctcacgg ccggcgagcg
     3301 ggtgcgcgtg gcctccgtgc cgacaagcga cctgcgagct ttccgactgt acaaggaggc
     3361 caggagcaac ctgtaccaga gacgactcga aacactgcgg ctcgcgcggc aacagttcgc
     3421 ggaagccatc gcactcgacc ctgcattcgc ggaagcgcac gccggacttg ccgagagctt
     3481 catgctcctg tcgatcaatc accaggacat tccggaaggt gaagcagcga ggcttgcgac
     3541 ctctagcgtc gaaaccgcgc tcgagctgga tccgaacctc gcggatgcct atgccgttcg
     3601 cgggctcatc aagatgaatg tttggtcgcg cacgagaatc gggccggaaa acatcgaagc
     3661 cgaggaagcg ttccgccgcg ccatcgcgct gaacccgagt catgccagcg cgtatatgtg
     3721 gtttgcgtcg ctgcgggatt ccgaagatcg gctcgaagat gcgatagagc tgtaccagag
     3781 atcgatggag ctggatcctc tcgcgagaat tccctactcc aaccttccga tggtgtatgc
     3841 gaagcgcggg gagcacaagg cagccatgcg tttgtggctg caagcgacgc gtatccacag
     3901 cgagtggccg acggtttacc agtacatagc agtgcagcta tggggtctcg gccggttcga
     3961 tgaggcatac gcgtggtatg cgaaagcgat ggaactcgga gccgcacccg gcatcagcgg
     4021 gaacgcggac gtggggatac tggtggatct gggcgacttg gagcaagccg aagccgtgct
     4081 ggggcgcttt gacgaatcgc attcattttt cccgtttgcg gaaggcatac gccagctgat
     4141 aaggggcgat tacgagcacg cggccactac cttttcaacc atcctggaca accggctgct
     4201 gccggaaaga tacgtgctgg agacggccgc gaacaccgcg ctccttgcgg gcgacctgga
     4261 atcggcgaaa aggtacaccc tggcggcaag cccgaccctc gcgggcgacg ccgcgggtaa
     4321 agtagaccga tcgtccgcca acaacgccat caagctcgct ttcatctacc agcacgaggg
     4381 ccgtgatgcc gaggcctcac tctacctgtc ggaacttctc gacctcatcc acgacgctcc
     4441 gcgactcggt acgttcggct acggcatccg cgatgtgcag atttatgcgc tccttgggcg
     4501 tcgcgaagat gccatcgcgg cgtttcgtga agcgctcgat gaaggctacc gcggatcggt
     4561 agtgttcgac ggctggccgc tctccatcga cccgttcctg gacaacatcc gtgacgaccc
     4621 gcgcttcatc gagatggtga ccgagctgga cagccacctc gcggtcatgc gggaccggct
     4681 gtacgcagcg cagctgtccg acggtctcga tgaactgcgg gcgcgtgccg gaacaacctg
     4741 atccggtcgt cctggacgat gaacctgtag cgatcccggg tatcccgcgg tttgaaagta
     4801 atgcggagtc agccgccgac gagtttgctg cggaacatga aaaagaccgc gccaaggatg
     4861 caaagagccg cccacaggta atcgagcttc aacggctcct tgaggtacag
//
LOCUS       DASUOM010000197         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_255185,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000197 DASUOM010000000
VERSION     DASUOM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255185"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFY27_04080"
     CDS             <1..342
                     /locus_tag="VFY27_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764720.1"
                     /translation="TTHWRYMILRRMGKPVEAERTLDAITADMNVIENTVYHRLCLFY
                     KGQLSLEDLALEDAGNPTGAAAAYGIANWFLYTGDQAEARKRLQELVDTAGWDAFGYI
                     AAEADLARMRL"
     gene            471..1367
                     /locus_tag="VFY27_04085"
     CDS             471..1367
                     /locus_tag="VFY27_04085"
                     /inference="COORDINATES: protein motif:HMM:NF038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor H family protein"
                     /protein_id="HEX5764721.1"
                     /translation="MYAYKNRKASAAVIAAVLALCIGEPSAADEQIPAPQAEVRESTT
                     LYFDKGGRERTSLKARTNDAFDPLVTTGLRKKPGPEAASGKTSSTTEAIESAKGSNDF
                     WFYYADVQLFSDDDNDGYFYGLDVLFDADTYFEVADVYAVLYLSYEGGPWNEYAVTEP
                     FSIYGATSDDEYVVVTELETGYPTGSYDLLIELYDTYDGAFVADIGPEDDSGLGFLPL
                     EDFNRDDPHRHDNHHRGGGGAIDWLTAVLLAGMLIHASYHRRRAAKVSDGLLRSLRAS
                     AGGFRREGMSARGPHHLASPCG"
     gene            complement(1287..>3030)
                     /locus_tag="VFY27_04090"
     CDS             complement(1287..>3030)
                     /locus_tag="VFY27_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5764722.1"
                     /translation="MFGTKTDISIFMTHVSVAIIVALVASLFIAQTLVPMLAARVPAP
                     PPAKDGAWMTRLTRRYARSLGWILGHPWWTGLGVVLICAVGVMPLVLNLVKFDAFPQD
                     AGRRLYMPYHLEGQYPLETVKSAVDRIEEYLFENQEEFNIRAVYSYYDKGEAGSTLLL
                     TDEEDATLSTTEVVERIKEKLPVIAIGKPSFQFDQQGGGEGFSLQVSGDSTSRLNELG
                     ADIVRLLSSIEGLEDIRSDADSGEREVRVSIDRIRAAQAGLTTKQIADAIAVAMRGQN
                     LREFRGESGEVEVRLAFRESDKQSIDQLGGLPLYTADGRRIRLGSVADFEMGFSPDAI
                     QRTDRQTGVVLSANLTGDATMDTVQPKVEALMGQFELPPGYSWKFGRGFERQDETQQM
                     MLQNILLGIACIFLVMAALFESLLFPFSIILGSIVFSIFGVFLLFAATGTTFTFMASI
                     GIMILIGVVVNNGIVLIDHVNNLRMQGLPRNEAVIEAGRDRLRPILMTVATTIVGLAP
                     LAVSTTQIGGDGPAYFPMARAIIGGLAFSTIVSLLVVPAFYVYFDTLSAWGRKVMRTA
                     RGHTLPAESAGAGA"
BASE COUNT          609 a          937 c          911 g          573 t
ORIGIN      
        1 acgacgcact ggcgctacat gatcctgcgc cgcatgggga aaccggtgga agcggagcgg
       61 acgctcgatg ctatcacggc ggacatgaac gtcatcgaga acaccgtcta tcatcgtctt
      121 tgtctttttt ataaggggca actgtcgctc gaggatctcg ccctggagga cgcgggaaat
      181 ccaacgggcg ctgccgcggc ctacggcata gcgaactggt ttttgtacac tggcgaccag
      241 gcggaggcgc ggaagcgcct gcaggaactc gttgacaccg cgggctggga cgccttcggc
      301 tacatcgccg cggaagcgga ccttgcgcgc atgcgcctgt agggactcga gctcccgggc
      361 aaattcgttc atttgccatt cataaatcac gctcgcggat tcagccccga ttcatcgtga
      421 ttgcccttta atacgtacag gcaccggatg tgcggaaaag gggacctgac atgtacgcat
      481 acaaaaacag gaaagcatcg gcggctgtca tcgccgcggt tcttgcgctt tgcatcggcg
      541 agccttccgc ggctgacgag cagattcctg caccgcaagc cgaggtccgg gaatctacga
      601 cgctctactt cgacaagggc ggacgcgagc gaacttccct gaaggccagg accaatgatg
      661 ctttcgatcc cctggttacg acaggacttc ggaaaaagcc gggaccggaa gcggccagcg
      721 gcaagacttc atccaccacc gaggcgatcg aatcggccaa gggatcgaac gacttctggt
      781 tctattacgc cgacgtgcaa cttttcagcg acgacgacaa tgacggctac ttttatggac
      841 tggacgtgct atttgacgca gacacttact tcgaagttgc cgatgtctac gcggtacttt
      901 acctgagcta tgaaggcggt ccttggaacg agtacgccgt gaccgaaccc ttcagcatct
      961 atggcgccac gagcgacgat gagtacgtgg tggtcaccga actcgagacc ggctacccga
     1021 ccggaagcta cgacctgttg atcgagcttt acgataccta cgatggcgcc tttgttgccg
     1081 acatcggccc ggaagacgat tccgggctgg gcttcctgcc gctcgaggac ttcaaccgcg
     1141 acgatccgca tcggcacgac aatcaccatc gcggcggcgg cggggccatc gactggctga
     1201 cagcagtcct gctcgctgga atgctgatcc atgccagcta ccaccgccgg cgagcggcga
     1261 aggtcagtga cgggctcctg cgatcgttac gcgccagcgc cggcggattc cgcagggagg
     1321 gtatgtccgc gcgcggtccg catcaccttg cgtccccatg cggataacgt gtcgaagtag
     1381 acgtaaaacg ccggcaccac gagcagggat acgatggtcg agaacgccag cccaccgatg
     1441 atggcgcgcg ccatggggaa gtaggctggg ccatcgccgc cgatctgcgt cgtactgacc
     1501 gccaggggcg cgagcccgac gatggtggtg gcaacggtca tcaggatcgg gcgtagccgg
     1561 tcgcgaccgg cttcgataac cgcctcgttc ctgggcaggc cctgcatgcg caggttgttt
     1621 acgtggtcga taagcacgat gccgttgttc actacgaccc cgataaggat catgatgccg
     1681 atcgaggcca tgaacgtgaa cgtcgtaccc gtcgcggcga acaggagaaa tacgccgaag
     1741 atcgagaaca cgatcgatcc caggatgatg gaaaacggaa acagcagtga ttcgaacagc
     1801 gccgccatca ccaggaagat gcacgcgatg ccgagcagga tgttctgcaa catcatctgc
     1861 tgcgtctcat cctgccgctc gaagccgcga ccgaatttcc agctgtagcc gggaggcaat
     1921 tcgaattgcc ccatcagcgc ctcgaccttt ggctgcactg tgtccatcgt ggcatctccc
     1981 gtgaggttcg cgctcagaac gacgcccgtc tggcggtccg ttcgctggat cgcgtccgga
     2041 gagaaaccca tttcgaaatc cgcgacgctt cccagcctga tccggcggcc gtctgccgtg
     2101 tacagtggca gcccgccgag ctggtcgatg ctttgcttgt cgctttcccg aaaagcgagg
     2161 cgaacttcta cttcgccgct ctcgccacgg aactcgcgca ggttctggcc gcgcatggcg
     2221 accgcgatag catccgcgat ctgcttcgtc gtgagcccgg cctgcgccgc gcgaatccgg
     2281 tcgatcgata cccgcacctc gcgttccccc gagtcggcgt ccgaacggat atcctcaagg
     2341 ccttcgatgc tcgagagcag gcgcacgatg tccgctccca gttcgttgag acgcgatgtt
     2401 gagtccccgg agacctgcag gctaaatcct tcgcctccac cttgctggtc aaactggaag
     2461 ctcggcttgc cgatcgcgat gaccggcagc ttttccttga tccgctcgac gacttccgtg
     2521 gtggagaggg tggcgtcctc ttcgtcggtg agcagcaagg tcgaccccgc ctcgcccttg
     2581 tcgtaatagc tgtacacggc gcggatgttg aactcttcct ggttctcgaa caggtactcc
     2641 tctatccggt ctacggcgct cttgacggtc tcgagcggat actgcccctc gagatggtag
     2701 ggcatgtaca gcctgcgacc cgcatcctgc gggaacgcgt caaacttgac gagattcagc
     2761 accaatggca tcacgccgac ggcgcatata agcaccacac cgagccccgt ccaccaggga
     2821 tggccgagta tccagcctag gctcctggcg tagcgccttg tcagcctcgt catccaggcc
     2881 ccgtctttcg caggcggcgg cgcggggacg cgggcggcga gcatgggtac gagcgtctgt
     2941 gcgatgaaaa gcgacgcgac cagcgccacg atgatggcaa cagagacgtg cgtcatgaaa
     3001 atggagatgt ccgtcttcgt accgaacata
//
LOCUS       DASUOM010000198         6855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1104398,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000198 DASUOM010000000
VERSION     DASUOM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104398"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFY27_04095"
     CDS             complement(<1..489)
                     /locus_tag="VFY27_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5764723.1"
                     /translation="MKRRREPETAKIDSLTHDGRGIAAIAGKKVFVPGALPGEEVRIL
                     RRKIRRGHDEADLLEVLKPSAERVVPRCEVFGICGGCALQHIDAADQRQMKQKSLKDS
                     LERIGGVSAERWLPPVFDESGENSWQYRRRARLAVRDVAAKGRVLVGFKERGAAMVTD
                     MHR"
     gene            complement(479..1255)
                     /locus_tag="VFY27_04100"
     CDS             complement(479..1255)
                     /locus_tag="VFY27_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5764724.1"
                     /translation="MHCFSFDIETIPDVEFGRRVYGLDGLNDEDVASAMAAIRQQAAG
                     SDFLPLYQHRVVAISVALRTRDTFHVWSLGDRDASEAELIGRFYDGLERYSPDLVSWN
                     GSGFDLPVLHYRALRHGIQAPRYWELGDSDREFRYNNYLSRFHWRHIDLMDVLSGFQP
                     RGGAKLDHIAAMLGFPGKLGMSGDKVWQRWLEGGIDDIRNYCETDVLNTYLIYLRFEF
                     MRGKLDATSLKRELDLVRSSLLALNLPHLNEFAAAWPDEA"
     gene            complement(1291..1671)
                     /gene="acpS"
                     /locus_tag="VFY27_04105"
     CDS             complement(1291..1671)
                     /gene="acpS"
                     /locus_tag="VFY27_04105"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5764725.1"
                     /translation="MIFGVGTDIVEIARMQRTYERFGERFVDRLLMPEERAYFDSSKW
                     PVRFLAMRFAGKEATVKAMGTGFAHGVWIRDVGIINNEWGRPLVIFSERGLSVCRELG
                     IGESHVSLTDDAGLVMAFAVAMRA"
     gene            complement(1668..2408)
                     /gene="pdxJ"
                     /locus_tag="VFY27_04110"
     CDS             complement(1668..2408)
                     /gene="pdxJ"
                     /locus_tag="VFY27_04110"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5764726.1"
                     /translation="MVRTEAQHLGVNIDHVATLRQARGTSYPDPVAAALLAEEAGADS
                     ITLHLREDRRHIQDRDVEALKETMSTHMNLEMAVTEEMLVIACRIMPSDCCLVPENRA
                     ELTTEGGLDVAARMDSVTAACRRLSDHGIRVSLFVDPEPRQLDAAREAGAPVVEIHTG
                     AYAEASDDRRDAELERIRQAAAYARQSGLTVHAGHGLHYGNVQRIAAIPEIVELNIGH
                     SIVARSVFDGMKAAVAEMKRLMNEARTR"
     gene            complement(2424..3170)
                     /gene="recO"
                     /locus_tag="VFY27_04115"
     CDS             complement(2424..3170)
                     /gene="recO"
                     /locus_tag="VFY27_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5764727.1"
                     /translation="MIRRVQNEPAWLLHHRDYQDSSRILELLSRDHGRLSVVARGSRS
                     AGSRLKGILRPFLPLQVSWVSRTDLGTLTGAELRGAPVSLYGDALLSGYYANELLLHL
                     LHRHDPQPEIFRIYGSTIESLSGGRDINAKLRTFEMELLRLLGYALNLDHDTESQAPL
                     SAARSYMYRPEQGPAVASDASGPMIFSGSDLIAIREGDFSVPETLRSAGRLLREVIAF
                     HLGGRELKSRKVFREMRRGSEPAGKDESQE"
     gene            complement(3195..4097)
                     /gene="era"
                     /locus_tag="VFY27_04120"
     CDS             complement(3195..4097)
                     /gene="era"
                     /locus_tag="VFY27_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5764728.1"
                     /translation="MAPGNAFRAGFVAVVGRPNVGKSTLVNALVGRKISIVTPKPQTT
                     RHRILGVHTSRHAQIIFVDTPGLHSEAGKAMNRLMNRTAINALQDADLILFVCEANRW
                     TDDDRNALERTRGVSAPVIALLNKIDKVHPKEELLGRIAAISEKAEFAEVIPVSAWRN
                     DNLDRLMAVIPRFLPESPPLFPADTFTDRSETFQVAELVREQLTLNLRQEIPYGLTVQ
                     VEQYKKTDDKLEVHAVIWVERESQKGIVVGKGGAVLKKIGRSVRLELKERLGVPVHIE
                     LWVKVKENWADNEEQLQRLGFDPG"
     gene            complement(4098..4778)
                     /gene="rnc"
                     /locus_tag="VFY27_04125"
     CDS             complement(4098..4778)
                     /gene="rnc"
                     /locus_tag="VFY27_04125"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5764729.1"
                     /translation="MDKAAAWLKHKLDYEFRDEQLLTQALTHRSSLGPNNERLEYLGD
                     AVLDVVVSEATYKLRPEASEGVLSRIRSSLVKDTMLTELATELDLASHLILGAGVKKG
                     SGYGRSSILGDALEAIFGAIYLDSGFDEARRVIRRVYGDRLVELPESASRRDPKTQLQ
                     ELLQARKLDLPQYRVENVSGQAHRQTFEVSCSIDALEASTIGRGSSRRRAEQDAAMKM
                     LALVGESA"
     gene            complement(4883..5311)
                     /locus_tag="VFY27_04130"
     CDS             complement(4883..5311)
                     /locus_tag="VFY27_04130"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX5764730.1"
                     /translation="MNWLENRNGFVAPGELQAAGRPATRQAGMTTVGFLILVVFVGLF
                     AFAFIRLTPVYLNYMKVAGVLDGVHKEFDSQNASRNAIRTSISRRFDVESVSQITAKD
                     IKVTTDSNGFLVQADYDHTAPFIGNVSFTVHFEKEVLVRR"
     gene            complement(5397..6158)
                     /gene="lepB"
                     /locus_tag="VFY27_04135"
     CDS             complement(5397..6158)
                     /gene="lepB"
                     /locus_tag="VFY27_04135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5764731.1"
                     /translation="MNINLALILTLLTAVTGAIVAFDRFYLQKQQGKKELNRALGTMV
                     DYSRSFFPVLLFVLVIRSFVFEPFRIPSGSMMPTLLQGDYIFVKKYSYGLRLPVLETK
                     IVETGEPERGDVVVFRLPQEPSVNYIKRVVGLPGDTVEYSNHRLSVNGKPVPLSPDPD
                     PPADELAPRFIEDLGDREHAILIAHPGNTIRDGVYEVPEGHYFMLGDNRDNSRDSRFL
                     GYIPETHLVGEAVRIWMHMDGLEVPDWNRIGMKIQ"
     gene            complement(6177..>6855)
                     /locus_tag="VFY27_04140"
     CDS             complement(6177..>6855)
                     /locus_tag="VFY27_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX5764732.1"
                     /translation="FEVLMRDGKVQYVDNPSKLPPINEIVELREPVITANILVPEKHV
                     GSVMKLCIEKRGVQKQMRYLGSQVSLVYEMPLAEVVLDFFDRLKSVSRGFASFDYTFE
                     RFDPAALVRLDILINKERVDALSMIVHRASADSRGRELVERMRELIPRQMFDVAIQAA
                     IGSQIIARSTVKALRKNVTAKCYGGDVTRKRKLLEKQKAGKRRMKQIGSVEIPQEAFL
                     AVLRTDK"
BASE COUNT         1300 a         2172 c         1957 g         1426 t
ORIGIN      
        1 gcggtgcatg tcggtcacca tggccgctcc cctctccttg aaacccacca gcacccggcc
       61 cttggcggcc acatcccgca ccgccagccg cgcgcgccgg cgatactgcc agctattctc
      121 cccggactca tcaaacaccg gcggaagcca ccgttccgca ctgaccccac cgatccgttc
      181 caggctgtcc ttcagcgact tctgcttcat ctggcgctgg tctgcggcat cgatatgctg
      241 cagcgcgcag ccgccgcaaa tgccgaatac ttcacagcgg ggcaccacac gctccgccga
      301 gggcttgagg acctcgagca ggtccgcctc atcgtgccca cgacgaatct tgcgccggag
      361 gattcgcacc tcctctcccg gcagcgcacc cggcacgaat actttcttgc ccgcgatcgc
      421 cgctattccc ctgccgtcgt gcgtgaggga atcaatcttc gcggtttccg gctcgcgtct
      481 acgcttcatc cggccatgcg gcagcaaatt cgttgaggtg gggcaggttc aaggccagga
      541 gagaggagcg tacgaggtcc agctcgcgct tcaggctggt ggcgtccagc ttgccgcgca
      601 tgaattcgaa gcggaggtat ataagataag tgttgagtac atccgtttcg cagtaattac
      661 ggatgtcgtc gataccgcct tcgagccagc gctgccacac cttatccccg ctcatgccga
      721 gcttccccgg aaagccgagc attgccgcta tgtggtcgag cttcgcacct cccctgggct
      781 ggaagcccga cagtacatcc atgaggtcga tgtgacgcca gtggaagcgg ctgaggtagt
      841 tgttgtagcg aaactcgcgg tcgctgtcac caagttccca gtaacgcggc gcctggatgc
      901 catggcgcag tgcgcggtaa tgaagcacgg gcaggtcgaa gccgctgccg ttccaggaca
      961 cgagatcggg tgagtagcgt tcgaggccgt cgtaaaagcg gccgatcagt tcggcctcgc
     1021 ttgcgtcgcg atcacccagg ctccagacgt ggaatgtatc ccgggtccgc agggcaacgg
     1081 agatcgcgac gacgcgatgc tggtagagtg gcaggaagtc gctgccggcg gcctgctggc
     1141 ggattgccgc catcgcggag gcgacatcct cgtcgttcaa cccgtcaagt ccatacacgc
     1201 gccgaccgaa ttcgacgtcg ggaatcgttt caatatcgaa tgaaaaacaa tgcattatgg
     1261 cgcttttatc aagtaataca tcacactcgc tcaggcccgc atcgccactg caaatgccat
     1321 gaccagcccg gcatcgtccg tcaggctcac gtggctctcg ccgatcccca gttcacggca
     1381 tacgctcagg ccacgctccg aaaagatgac cagcggcctg ccccactcat tattgatgat
     1441 gccgacatcg cgtatccaga cgccatgagc gaagcccgta cccatcgcct tcactgttgc
     1501 ctccttgccg gcaaatcgca tggcgaggaa gcggaccggc catttgctgc tgtcgaagta
     1561 tgcccgctcc tccggcatca gcagccggtc gacgaaacgc tcgccgaaac gttcgtaggt
     1621 gcgctgcatg cgggcgatct cgacgatgtc tgtgccgaca ccgaaaatca ccgggttctc
     1681 gcttcgttca tgaggcgctt catctctgcg accgccgcct tcataccatc gaaaaccgag
     1741 cgcgcgacga tggaatggcc gatgttgagt tcgacgatct ccggaatcgc ggcgatccgc
     1801 tgaacgttgc cgtaatgcag gccgtggccg gcgtgcaccg tcagccccga ctgccgcgcg
     1861 taggcggcag cctgcctgat acgttccagt tccgcatcgc gtcgatcgtc gcttgcctct
     1921 gcgtaggccc ccgtatgtat ttccaccacc ggggcgcctg cttccctggc cgcatcgagc
     1981 tggcgcggct ccggatccac gaacagcgaa acccggatgc cgtgatcgga cagcctcctg
     2041 cacgcggccg tgacgctgtc catccgtgcg gcgacgtcga gtcccccttc cgtcgtgagc
     2101 tccgcacggt tctccggcac gaggcaacag tcggaaggca tgatgcggca ggcgatgacg
     2161 agcatctctt cggtgactgc catttccagg ttcatatgcg tggacatggt ttctttcaat
     2221 gcctcgacat ccctgtcctg gatatgccgg cgatcctctc gcaggtgaag agttatgctg
     2281 tcggcacccg cctcctccgc aaggagcgct gctgcaaccg ggtcgggata gctcgtcccg
     2341 cgcgcctgcc tcagtgtcgc gacatggtcg atgtttacgc cgagatgttg tgcttcagtt
     2401 ctcaccatac cgtcccgtca ttgtcattcc tgtgactcgt cttttcctgc aggctcgctt
     2461 cctcgccgca tctcgcgaaa cactttgcgg cttttcagtt ccctgccgcc caggtgaaac
     2521 gcgatgactt cccgaagcag gcgccccgcg gagcggagcg tttccgggac cgaaaaatca
     2581 ccttcgcgga tggctatgag gtcactgccg gagaagatca ttggcccgga cgcgtcgctc
     2641 gcgaccgccg gtccctgctc cggccggtac atataagagc gtgctgccga gagcggcgcc
     2701 tgcgattccg tatcgtgatc caggttcagc gcgtagccta gcagacgcag cagctccatt
     2761 tcgaatgtgc gcagtttggc attgatgtcg cggccgccgg acaggctctc tatggtggac
     2821 ccgtaaatcc tgaaaatctc cggttgcgga tcgtgccggt gcaaaaggtg cagcaacagc
     2881 tcgtttgcgt agtatcccga cagcagggca tctccgtaca gggaaaccgg cgcaccacgc
     2941 aactcggcac ccgtcagtgt tccgagatcg gtgcgactga cccaggaaac ctgaagcggg
     3001 aggaacgggc gcaggatccc cttcaggcgc gacccggctg aacggcttcc cctggctaca
     3061 acggacaggc gcccgtgatc acggctcaag agttccagta tgcgactgga gtcctggtaa
     3121 tcgcgatgat gcaacaacca cgccggttcg ttctgtacgc gacgaatcat gatctggtac
     3181 cgcgcaatgg cctttcagcc agggtcgaag ccgagcctct gcagttgttc ctcgttgtcc
     3241 gcccagttct ccttgacctt cacccagagc tcgatgtgca ccggcactcc cagccgctcc
     3301 ttcagctcca gccgcactga gcggccgatt tttttgagca cggccccgcc tttgcctacc
     3361 acgatgcctt tctggctctc ccgctcgacc cagatgaccg catgcacctc gagcttgtcg
     3421 tccgtctttt tgtattgctc gacttgcacc gtaagcccgt acggaatctc ctggcggaga
     3481 ttcagggtca gttgctcgcg gacgagttcg gcaacctgga aggtctcgct cctgtcggtg
     3541 aacgtatcgg cagggaacag cggcggcgac tctggcaaga accgcgggat gaccgccatc
     3601 aacctgtcca ggttgtcgtt acgccatgcc gatacgggaa tgacttccgc gaattccgct
     3661 ttctccgaaa tcgcagcaat cctgccgagc agttcttctt tcggatgaac tttgtcgatc
     3721 ttgttcaaca aggcgatcac gggcgcggat acaccgcgcg tgcgttcgag cgcgttccgg
     3781 tcatcgtccg tccagcggtt tgcctcgcac acgaacagga taaggtcggc atcctggagc
     3841 gcattgatcg cggtccggtt catcaaacgg ttcatggcct tcccggcctc actgtgcagg
     3901 ccgggagtat ccacgaagat gatctgcgcg tgccgggatg tatggacgcc gagaatacgg
     3961 tggcgcgtcg tctgcggttt cggcgtcacg atgctgatct tcctgcccac cagggcattg
     4021 acgagcgtcg acttgcctac gttcggacgg cccacgactg ccacgaaacc tgcgcgaaaa
     4081 gcattccccg gcgccattca ggcggactcg ccaacgagcg ccagcatttt catggcagca
     4141 tcctgttcgg cgcgacgtcg cgagctcccg cgcccgatcg tgctcgcctc cagcgcatcg
     4201 atactgcaac ttacttcaaa agtctggcgg tgcgcctgtc cgctgacgtt ctctacccgg
     4261 tactgcggca ggtccagctt gcgcgcctgc aacaactcct gcaactgcgt cttcgggtcc
     4321 ctgcgagacg ccgactccgg aagctccacc agtcgatcgc cgtagactct gcggatgacg
     4381 cgccgtgcct catcgaaacc agagtcgaga tagatcgccc cgaaaatcgc ctcgagggcg
     4441 tcaccgagta tggaggagcg gccgtaaccg ctgcctttct taacgcctgc gccaaggatc
     4501 aggtgcgagg cgaggtccag ctccgtggcc agctccgtca gcatcgtgtc cttgaccagg
     4561 gatgaacgaa tgcggctcaa gactccctcg ctcgcctccg gacgcagttt gtaggtcgct
     4621 tccgatacca cgacatcgag gaccgcatcg ccaagatatt cgagtcgttc gttgttaggg
     4681 ccgaggctac tgcggtgggt gagcgcctgg gtcagcaact gctcgtcacg aaattcgtaa
     4741 tccagcttgt gcttcagcca ggctgcagct ttatccaaaa taatggctca ggaggcaggt
     4801 tcgggaaccg ccattgtcga tcaaaagaga ccgtagtcca agccgctcgc aggaggcgac
     4861 ggctgtcgga aggcgccgtt cttcagcggc ggacgagcac ttccttctcg aaatgcacgg
     4921 tgaaggatac gttgccgata aaaggcgccg tatggtcgta gtcggcctgc acgagaaagc
     4981 cgttgctgtc cgtcgtcacc ttgatgtctt tagcagtgat ctggctcacg ctctcgacgt
     5041 cgaagcgcct ggatatcgac gtgcgaatcg cattgcgtga cgcgttctgg ctgtcgaatt
     5101 ccttgtgtac tccgtccagc actccggcga ccttcatgta gttcaggtaa accggcgtca
     5161 ggcgtatgaa ggcgaaggcg aacaggccga cgaagacgac gagaatgagg aaaccgacgg
     5221 tggtcatacc ggcctgtcgc gtggcaggtc gccctgccgc ctgtaattcc ccaggcgcta
     5281 cgaaaccgtt ccggttttcg agccagttca tcgctgttcc tccgctccgc aagtgcgcga
     5341 taccccttgg ctaaggtagc gtgattttaa ccgcggaatc agtgactcgc accacattat
     5401 tggatcttca ttccaatgcg gttccaatcc ggaacctcga gcccgtccat gtgcatccag
     5461 atgcgaacag cctcgccgac caggtgagtt tccgggatgt agcccaggaa tctactgtcc
     5521 ctgctattgt ccctgttgtc ccccagcatg aagtagtgtc cctccggaac ttcatacaca
     5581 ccatcacgga tcgtgttgcc agggtgcgcg atcaggatcg catgctcccg gtcgcccagg
     5641 tcctcgataa accgtggtgc tagctcgtcg gccggcgggt cgggatcggg tgagagcggc
     5701 accggcttgc cgttgaccga caagcgatgg ttcgagtact ccaccgtatc tccaggcaag
     5761 ccgactaccc gcttgatgta gttcacgctt ggctcttgcg gcagccggaa gacgacgacg
     5821 tcgccgcgtt ccggttcccc ggtttcaacg attttcgttt ccaggacagg caagcggagt
     5881 ccgtaggagt acttctttac gaagatgtaa tccccctgca gcagcgtcgg catcatggaa
     5941 ccggaaggta tcctgaacgg ctcgaatacg aaggaacgaa tgaccaggac gaacagcaac
     6001 accgggaaga acgacctcga gtaatcgacc atcgtgccga gcgcgcggtt cagctccttc
     6061 ttgccttgct gcttctgcag gtaaaagcga tcgaacgcga cgatagcccc ggtcacggcg
     6121 gtcagcaggg tcagtatcaa tgccaggtta atgttcaata ggtatatctc agcttcttac
     6181 ttgtctgttc gcaatacggc gaggaatgcc tcttgcggta tttcgacgga accaatctgt
     6241 ttcatgcggc gtttacccgc tttctgcttt tccagcagct ttcgcttgcg cgttacgtca
     6301 ccgccatagc atttggcagt gacgtttttc ctgagcgcct tgacggtact gcgcgcgatg
     6361 atctggctgc cgattgccgc ctggatggcg acgtcgaaca tctggcgggg aatcagctcc
     6421 cgcattcgct cgaccagttc gcgcccgcgt gaatcggccg atgcgcgatg cacaatcatc
     6481 gacagcgcgt cgacgcgctc cttgtttatc aggatgtcga gtcgcaccag cgccgcggga
     6541 tcgaaacgct cgaaggtgta gtcgaacgaa gcgaagcccc ggctcacgga cttgagacgg
     6601 tcgaagaaat cgagtacgac ctccgcaagt ggcatttcgt acaccagcga aacctggctg
     6661 cccaggtagc gcatctgctt ctgcacgccc cgcttctcga tacagagctt catcaccgaa
     6721 cccacatgct tctcgggcac gagtatgttc gccgtgatga ccggttccct gagctcgaca
     6781 atttcgttga ttgggggaag ctttgacgga ttatccacat attggacctt cccgtcccgc
     6841 atcaggactt cgaat
//
LOCUS       DASUOM010000199         6290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1278908,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000199 DASUOM010000000
VERSION     DASUOM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278908"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFY27_04145"
     CDS             complement(<1..149)
                     /locus_tag="VFY27_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019458972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5764733.1"
                     /translation="MDAQSIKDFAARYDPQPFHLDEEAASDTIFRGLAASGWHTAAVT
                     MRMLV"
     gene            346..1803
                     /locus_tag="VFY27_04150"
     CDS             346..1803
                     /locus_tag="VFY27_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5764734.1"
                     /translation="MNPPHAMLRKGKYLLLLFVLGACTEGTNAQQEQDEPQELRNPQP
                     AVVDARALPDFRPLVREYGDAVVNVEVLQRRERRDVRGMPEDPMYEFFRRFGIPPPGF
                     EQRGEPPPARGAGSGFIVSEDGYILTNAHVVSGADQVTVRLTDRREFQAQVIGVDPRT
                     DVAVIKIDATDLPTVRIGDPEAVEPGQWVLAIGSPFGLENSVTAGIISATSRAVGPGS
                     NVPFIQTDVAVNPGNSGGPLFNLQGEVVGINSMIFSQTGGYMGLSFAIPIDVATNVQQ
                     QLIENGRVVRGRIGVIVQDVNAQLAQSFGLDRPRGALISGVEEGAPAAEAGLRPGDVI
                     LSLNDEPIERFTELGAAIAEMQPGTEATLGIWRNREEREIDVQVEEMEDASAEIAANS
                     PDGQAEEQLGLVVRPLTAEEKQMTGSEGSIVIEQVQGAAAAAGLQPGDIILGVNSERI
                     ESVQQLREAVEESPGNVALLVQRDQATIFVPVPTE"
     gene            1906..2925
                     /locus_tag="VFY27_04155"
     CDS             1906..2925
                     /locus_tag="VFY27_04155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5764735.1"
                     /translation="MIPLSILDLAFVRHGATPAEALGNSLDLAQHAERWGYRRFWLAE
                     HHNMIGIASAATSVVIGYIAGGTRSIRVGAGGVMLPNHAPLIIAEQFGTLETLYPGRI
                     DLGLGRAPGTDQATVRALRRSPASAESFPQDVLELQTLLGPQQPGQAVLAVPGAGLEV
                     PLWMLGSSLFGAQLAAMLGLPYAFASHFAPGALMQAIEIYRARFESSKQLDRPYVMVA
                     ANVVVADSDAEARRLFTSIQQQFANLVRGARSRLPEPIEDIEAYWSPLERAEADRMLA
                     CSFVGSKETVRKGIDDFISQTKADEVIIASAIFDHAARLRSYELLAEVHAESARTDAR
                     IDGHN"
     gene            2938..3690
                     /locus_tag="VFY27_04160"
     CDS             2938..3690
                     /locus_tag="VFY27_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5764736.1"
                     /translation="MNRVGKFGGRMATRIFRGLAWLGSHEFSVLLSAVVLSAGVWVFI
                     ELADEVTENEAVSIDEAVLLSLRNAGDLSDPVGPGWVEEMGRDFTALGSMGVLTLITL
                     AVLGYLLLAGRNRTSLFTLLAVAGGMLLSTLLKMGFDRARPDLVPHDTVVYTASFPSG
                     HSMMAAVTYLTLAAILSRVHSSPLLKAYLLITAAVITLLVGASRVYLGVHWPTDVLAG
                     WAAGAAWASLCWLIARGLQRRHMVEEQDSEGL"
     gene            3883..5070
                     /locus_tag="VFY27_04165"
     CDS             3883..5070
                     /locus_tag="VFY27_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5764737.1"
                     /translation="MTQYSRNPFLLPLFLLLVTGGCEEEQAPPPQQGAPAVTVVTLKT
                     EPVELTRQLPGRTTAYLIAEVRPQVTGIVEERLFEEGSVVEAGQPLYQLDDATYRARY
                     NSARASLARAEATVEVARLNAARAEELVKANAVSRQEYENAVAALQQAEADVGVAEAD
                     VASSRVELDYARITSPIAGRIGKSSVTQGALVTADQATPLATVQQLDPIYVDLTQSAS
                     ELLQLRQAVAGGTVRGTEDTPVTILLEDGTPFQHEGVLGFSDVSVDPSTGSFSLRVTV
                     PNPEHLLMPGMYVRAVLSTAVLEDGILVPQQGIARDAKGNANALVVGNDGTVEQRTVE
                     VARTIGAKWLVRSGLMAGDRVIVEGVQKVQPGMPVEATEAPSAAADAEPAVSPETTAA
                     DRQ"
     gene            5083..>6290
                     /locus_tag="VFY27_04170"
     CDS             5083..>6290
                     /locus_tag="VFY27_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5764738.1"
                     /translation="MAQFFIGRPIFAWVIAIIIMIGGALSMTQLPVSMYPTIAPPTVS
                     INANYPGASARVVEDSVTQVIEQNMKGLDGLLYMSATSSSSGSASVTLTFDNAVNPDI
                     AQVQVQNKLQLAMPLMPQEVQRQGVNVSKASSGFLMVIGFVSEDGRMTKDDIADYVSA
                     NIVDPISRVPGVGNLQVFGSQYAMRIWLDPNKLDTYRLTPTDIIAAVQAQNQQVAVGQ
                     LGGTPAVEGQQLNATITAQSRLQTAEEFRDIVVRSNPDGSVLKLADVARVELGAQNYG
                     FITRHNQRTASGLAVMMATGANALETARAVEARLSELEPFFPEGLTTVIPFETTPFVR
                     AAIEEVITILIEAIVLVFLVMYLFLQNFRATLIPTIAVPVVLLGTFGVLAVLGLSVNM
                     LTMFAMVLAIG"
BASE COUNT         1205 a         1916 c         2035 g         1134 t
ORIGIN      
        1 tccacgagca tgcgcatggt cacggcagct gtgtgccatc cactggcggc cagtcctctg
       61 aagatcgtgt cactggcggc ttcttcatcc aggtgaaacg gctgcggatc gtacctcgct
      121 gcaaaatcct tgatggactg cgcgtccatg acgatgcgcg tgtcacttct gaacacctgg
      181 ccgaccgtaa ggtcctcgaa ataaagctgt agcaagtcag tttccaccgt gaaggagcag
      241 gagcccgcat cataccgccc gtcgccaggg ggaagagtgg tggcatgaat cctgcaaaag
      301 ccccggaagc gagagattcc tgagacgcac gatggagtga atgccatgaa tccgccacac
      361 gcaatgcttc ggaagggcaa atacctgctc ctgcttttcg tgcttggcgc ctgcaccgaa
      421 ggaacgaatg cacagcagga acaggatgaa ccgcaggaac tgcggaaccc ccagccggcg
      481 gttgtcgatg cccgcgcctt gcctgacttc cgtccgctgg tccgcgaata cggcgatgcc
      541 gtggtgaatg tggaagtgct gcagcgccgt gaacgacgcg acgtccgggg catgccggaa
      601 gacccgatgt acgaattctt tcgccgcttc ggcataccgc cgcccgggtt cgaacagcgc
      661 ggcgaacctc cgccggcacg tggcgcgggt tccggtttca tcgtgtcgga ggacggttac
      721 atcctcacca acgcgcacgt cgtcagcggt gcggaccagg tcaccgttcg cctgaccgac
      781 aggcgggaat tccaggcgca ggtcataggc gtcgaccccc gtaccgatgt ggccgtcatc
      841 aagatcgatg ccaccgacct gccgacggtc cgcatcggcg accccgaggc cgtggaaccg
      901 ggccaatggg tgctggcgat cggttcaccg ttcgggctcg agaacagcgt caccgccggc
      961 atcatcagcg caacctcccg cgccgtcggc ccggggagca acgtaccgtt catccagacg
     1021 gacgtcgctg tcaatcccgg caattcgggg ggaccgctgt tcaacctgca gggagaggtt
     1081 gtcggcatca actcgatgat cttcagccag accggcggct acatggggct ttcgttcgcc
     1141 atacccatcg acgtggcgac aaacgtgcag caacaactga tcgaaaacgg ccgcgttgtg
     1201 cgcggacgca taggcgtcat cgtgcaggac gtaaacgcgc agctcgcgca gtcgttcggc
     1261 ctcgacaggc cccgcggcgc tctcatcagc ggcgttgaag aaggtgctcc cgcggccgag
     1321 gccgggctgc gtcccggtga cgtcatcctg agtttgaacg acgaaccgat agagcgattc
     1381 accgaactcg gtgctgcgat tgctgaaatg cagccgggga cggaggccac gctcgggatc
     1441 tggcgcaatc gcgaggaacg cgagatagac gtccaagtcg aggagatgga ggacgcttcc
     1501 gcggaaattg ccgcgaattc gccggacggg caggcggagg aacagctcgg cctggtggtg
     1561 cgaccgttga cggcggaaga gaagcaaatg accggctccg aagggagcat cgtgatcgag
     1621 caggtccagg gcgctgccgc ggccgccggg ttgcaaccgg gcgatattat tctcggcgtc
     1681 aacagcgaac gtatcgaatc cgtccagcaa ctgcgagagg ccgtggaaga atcgccaggg
     1741 aatgtagcac tgctggtcca gcgcgatcag gcgacgatct tcgtaccggt accgaccgaa
     1801 taatatggcg acaaggtccg ggaatggccc ccggcgacgg actccggggg ccattggtct
     1861 ttgttggtct tcggcgcggt tcttgccctg ttgcccgggt aaacgatgat cccgctttcc
     1921 atcctcgacc tcgcgttcgt gcgccacggt gcaacgccgg cggaggccct gggaaattcg
     1981 ctcgatctcg cgcaacacgc cgaacggtgg ggatatcgcc gcttctggct ggcggaacac
     2041 cacaacatga tcggcatcgc gagcgcggcc acatcggtgg tcatcggcta catcgcgggc
     2101 ggaacgcgca gcattcgcgt cggcgcgggc ggcgtcatgc tgccgaacca cgccccactg
     2161 atcatcgcgg agcagttcgg gacgctggag acgctttatc ccggccgcat cgatttgggc
     2221 cttggacggg ctcccggtac cgaccaggcg acggtgcggg cgctgcggcg gtctccggcg
     2281 agtgccgaaa gttttccaca ggatgtcctt gaactgcaga cgctgcttgg cccgcaacag
     2341 cctggccagg cggtactggc agtgccggga gcgggactcg aagtgcccct ctggatgctg
     2401 ggttccagtc tcttcggcgc acaactggcg gcgatgctcg ggctgcccta cgcgttcgcc
     2461 tcgcacttcg cgccgggcgc gctgatgcag gctatagaga tttaccgcgc ccgcttcgag
     2521 tcgtcgaagc aactcgaccg cccgtacgtg atggttgccg ccaacgtggt ggtcgcagac
     2581 tcggacgccg aagcgaggcg cctgtttaca tcgatccagc agcagttcgc aaacctcgtg
     2641 cgaggtgccc gaagccgatt accggagccc atagaagaca ttgaagcgta ctggtccccg
     2701 ctcgagagag ccgaagcgga tcggatgctt gcctgttcat tcgtcggctc gaaggaaacg
     2761 gtgcgcaagg gaatcgacga tttcatctcg caaacgaaag ccgacgaagt aatcatcgcg
     2821 tcggctatct tcgatcatgc ggcacggctg cgttcgtacg agttactcgc ggaagttcat
     2881 gcagaatccg ctcgaaccga tgctcgtatt gacggccaca attgaatgcc tgctgtgatg
     2941 aacagggtcg gcaagttcgg cggacgtatg gctaccagga tcttcagggg gcttgcgtgg
     3001 ctcgggtctc acgagttctc agtcctactg tcggcagtag tgctgtcagc aggcgtatgg
     3061 gtgttcatcg aacttgctga cgaggtgacg gaaaacgagg ctgtgagcat cgacgaggca
     3121 gtactgctct cgctgcggaa cgcgggggac ctgagcgacc cggtcgggcc gggctgggtg
     3181 gaggaaatgg ggagagactt taccgcgctc gggagtatgg gcgtgctgac cctcattacc
     3241 ctcgcggttc tcggctacct gttgctggcg gggcggaacc gcacctctct gtttacgttg
     3301 cttgccgtcg ccggtgggat gttgctgagc acgttgttga aaatgggctt cgaccgcgcc
     3361 aggcccgacc tggtgccgca cgacacggtc gtctataccg cgagtttccc gagcggccat
     3421 tcgatgatgg cggcagtgac ttacctgacc ctggccgcga tcctgagccg cgtgcacagc
     3481 tcgccgctcc tgaaggcata tctgctgatc acggcggccg tcatcaccct gctcgtaggt
     3541 gcaagccgcg tttacctggg cgtgcactgg ccgaccgacg tgctcgcggg ttgggccgcg
     3601 ggcgcggctt gggcctccct ctgctggctc atcgcgcggg ggctgcagcg ccgacacatg
     3661 gtggaggagc aggactccga aggtctttaa ggatttccgg aaaagcgtcg gaacagggga
     3721 cattccgagg aaaattgaac aacggtcgcc gcatttgggt caacttggag ggttgggggg
     3781 ctggaaacgc cttccgggag gggcttgaca aaaagagaat gaacgttcat tctccgcgcc
     3841 ccgctgcaaa ataagaccac ctgcgagcga gcctcaagtc caatgaccca gtattcgcgt
     3901 aacccgtttc tcctgccgtt gttcctgttg ctggtgaccg gcgggtgcga ggaagaacag
     3961 gcaccgccgc cgcaacaggg cgcgcccgcc gtcaccgtcg ttacgttgaa gaccgagcct
     4021 gtcgagctta cacgacagtt gccgggccgc acgaccgcct acctgattgc ggaagtccgt
     4081 ccacaggtga cggggatcgt cgaggagcgc ctgttcgagg agggcagcgt ggtagaggcg
     4141 ggacagccgc tctatcagct cgatgacgca acgtacaggg caaggtacaa cagtgcgcgc
     4201 gcttcgctcg cccgcgcgga ggcaacagtc gaagtcgcaa ggctgaatgc ggctcgcgcc
     4261 gaagaactgg tcaaggcaaa tgccgtcagc cgccaggaat acgagaacgc agtcgccgca
     4321 ttgcaacagg cggaagctga cgtcggcgtc gccgaagcgg acgtcgcaag cagccgcgtt
     4381 gagctggact atgcccgcat tacctcgcct attgccggac gtataggtaa gtcttcggtc
     4441 acgcagggag cgctggtcac cgcggatcaa gccacgcccc tggcgaccgt acaacagctc
     4501 gacccgatct acgtcgacct gacgcagtcg gccagcgaac tgctgcagtt gcgccaggcc
     4561 gttgccggcg gcaccgtgcg cggaactgaa gacactccgg taaccatcct tctcgaggac
     4621 gggactccct tccagcatga gggtgtactc ggtttctctg acgtgagcgt ggatccgtcg
     4681 accggcagct tttccctgcg cgtcaccgtg ccgaatccgg agcacctgct gatgccggga
     4741 atgtatgtcc gcgcggtatt gagtacagcc gtgctggagg acgggattct cgtgccgcag
     4801 cagggcattg cccgtgacgc gaaaggcaac gccaatgccc tggtcgtagg gaatgacggc
     4861 acggtcgagc agcgcaccgt cgaggtcgct cgcaccatcg gcgccaagtg gctggtgaga
     4921 agcggactga tggccggtga tcgcgtcatc gtggagggcg tgcagaaggt ccagccggga
     4981 atgccggtcg aggccaccga agcgccgtcg gctgcagccg atgcggagcc ggcggtttcg
     5041 cccgaaacga ccgccgctga ccggcaatag gggcaagtcc acgtggctca gttctttatc
     5101 gggcgcccga tctttgcctg ggtaatcgcc atcatcatca tgatcggcgg cgcgctgtcg
     5161 atgacgcagc tgccggtgtc catgtatccg acgatcgctc cgcctacggt atcgatcaac
     5221 gccaactacc cgggagcctc ggccagggtc gtcgaagatt cggtgacgca ggtcatcgag
     5281 cagaacatga aggggctgga tggccttctg tatatgtcgg caaccagctc ctcgagcggc
     5341 agcgcttccg tgacgctcac cttcgacaac gccgtaaatc cggacatagc ccaggtgcag
     5401 gtgcagaaca agctgcagct cgcgatgcca ctgatgccgc aggaagtgca gcgccagggc
     5461 gtcaacgtga gcaaggcgag cagcggcttc ctcatggtga tcggcttcgt ctccgaagac
     5521 ggcaggatga cgaaggatga catcgcggat tacgtttctg cgaacattgt cgacccgatc
     5581 agccgcgtac cgggcgtcgg caacctgcag gtattcggat cccagtatgc gatgcggatc
     5641 tggctggatc cgaacaagct ggatacgtac cgcctgactc ctaccgacat catcgctgcc
     5701 gtgcaggcac agaaccaaca ggtcgctgta gggcaattgg gcggcactcc cgctgtcgag
     5761 ggtcagcaac tcaacgcaac catcaccgcc cagagccgtt tacagacggc ggaggaattc
     5821 cgcgacatcg tcgtccggag taaccccgac ggttccgtgc tgaagcttgc cgacgtcgcg
     5881 cgcgtcgaac tcggcgcaca gaattacggc ttcatcaccc gtcataacca gcgcaccgca
     5941 agcggcctgg cagtgatgat ggccacgggc gcgaatgcgc tcgagaccgc gcgcgccgtc
     6001 gaagcgcggc tttctgaact ggaaccgttc ttcccggaag gacttaccac ggtcatcccg
     6061 ttcgagacga cgcctttcgt cagggccgcc atcgaggagg tcatcacgat cctcatcgag
     6121 gcgatcgtgc tggtgttcct cgtgatgtac ctgttcctgc agaacttcag ggccacgctc
     6181 ataccgacca ttgcggtccc ggtggtgctg ctgggcacgt tcggcgttct cgcggtgctc
     6241 ggcctgtcgg tcaacatgct gaccatgttc gccatggtgc tggcgatcgg
//
LOCUS       DASUOM010000200         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_29271,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000200 DASUOM010000000
VERSION     DASUOM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29271"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFY27_04175"
     CDS             complement(<1..395)
                     /locus_tag="VFY27_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX5764739.1"
                     /translation="MSITINSFKAYDIRGQIPNDLNPDIAYRIGNATVEFLGARTMVL
                     GRDIRLTSAEFADAVAAGMREAGADVLDIGVGGTEMVYFATGDLQADGGIMVTASHNP
                     ADYNGLKLVREQARPISGDTGLVDIRKIA"
     gene            complement(492..1928)
                     /locus_tag="VFY27_04180"
     CDS             complement(492..1928)
                     /locus_tag="VFY27_04180"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX5764740.1"
                     /translation="MSVIHPVILSGGSGTRLWPASRAMYPKQLLPLTGELTMLQQTAL
                     RLKGKQNVSATTLVVCNEAHRFLVAEQLRDIGVNATIVLEPEGRNTAPAVALAALLTR
                     RNSGSGAEPCLLLVMPADHVIENTDAFVTAVEAGEQSAIDGNLVTFGIVPSYAHTGYG
                     YIEADVHSLAAGPIKSFIEKPDKQAALRLLESNRFFWNSGIFLFRADRYLDELEKYMP
                     DMVDACKKSIELAKIDTDFVRPDAESFKASPSDSIDYAVMEKTARAAMVPLDAGWSDV
                     GSWAALHDVSSKDAQGNAFRGDVVAYNCTDSYISAQNRLVAAVGLKDIVVVEDKDSVL
                     VTPKDQSQDVKELVEELRRRGREETKLHRQVFRPWGSYDSIDSEDGFQVKRLIVKPGA
                     VLSLQKHARRAEHWVVVKGTARITRDDDVFDLGVNESTYIPIGTVHRIANPGDEPVHI
                     IEVQCGDYLGEDDIVRLEDNYGREGTNT"
     gene            complement(2016..>2411)
                     /locus_tag="VFY27_04185"
     CDS             complement(2016..>2411)
                     /locus_tag="VFY27_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX5764741.1"
                     /translation="TDDMRDAPSRVLMEALWKEGALVRAYDPEAMDETRRLYPDSKGL
                     TLCANAIEAITGADALAIVTEWQEFRSPDFPVLKQQLSDAVIFDGRNLYDPAMVEALG
                     LAYYGIGRGRSVNSASELSPAPLQAVGKA"
BASE COUNT          425 a          767 c          721 g          498 t
ORIGIN      
        1 tcggcgattt tgcggatgtc gaccaacccg gtgtcgccgc tgatcgggcg cgcctgctcg
       61 cgtaccagct tcaggccgtt gtagtcggcg ggattgtggc tggcggtgac catgatgccg
      121 ccatccgcct gcaggtcgcc ggtggcgaag taaaccatct cggtgccgcc gacgccgata
      181 tctagcacgt ccgcgccggc ttcgcgcata ccggcagcaa cggcgtccgc gaactctgcg
      241 ctggtaagcc ttatgtcacg ccccagcacc atggtcctgg ctccgaggaa ctcgacggta
      301 gcgttgccga tacggtaggc gatgtcgggg ttgaggtcgt tggggatctg tccccggatg
      361 tcgtaggcct tgaagctgtt gatggtgatg ctcatgttgc gatttatttc ctgtggtcgg
      421 aagtcaggga ccggtaactt tacccggcat tatcgcactc gcccgcatgg cgggctccta
      481 cgcaaaaccg cctacgtatt tgtgccctcg cgcccgtaat tgtcctccag gcgcacgata
      541 tcatcttctc cgaggtaatc accgcactgc acctcgatga tatgcacggg ctcgtccccg
      601 ggattggcga tccggtgtac cgtgccgatg ggtatgtagg tcgattcgtt gacgccgaga
      661 tcgaatacgt cgtcgtcgcg cgtgatccgc gccgtgcctt tcaccaccac ccagtgctcg
      721 gcccgccggg catgtttctg cagcgacagc accgcgcccg gtttgacgat aagccgcttg
      781 acctggaatc cgtcctcgct gtcgatggaa tcgtagctgc cccaggggcg aaacacctgg
      841 cgatggagct tcgtctcctc cctgccccgc cgcctgagct cctcgaccag ctccttgacg
      901 tcctgcgact ggtcctttgg cgtcacgaga acgctatcct tgtcctcgac cacgacaatg
      961 tccttgagac ccaccgcggc gaccagccgg ttttgcgcgc tgatgtagct gtctgtacag
     1021 ttgtaagcaa cgacatctcc acgaaatgcg ttgccctggg catccttgct gctgacgtcg
     1081 tgcaatgccg cccaggagcc gacgtcgctc catccggcat cgagcggcac catggcggca
     1141 cgtgccgtct tttccatgac cgcgtagtcg atcgaatcag acgggctggc cttgaaagat
     1201 tcggcatcgg ggcgtacgaa atcggtgtct atcttcgcga gctcgatgct cttcttgcag
     1261 gcgtcgacca tgtcgggcat gtacttctcg agttcgtcga gatagcgatc cgcacgaaac
     1321 aggaaaattc cgctgttcca gaaaaaccgg ttcgattcca gaaggcgaag tgccgcctgc
     1381 ttgtccggct tttctatgaa cgacttgatc ggaccggctg caaggctgtg cacgtccgcc
     1441 tcgatgtacc catacccggt gtgcgcgtaa gacggcacga tgccgaacgt caccaggttg
     1501 ccatcgatgg cagactgctc tcccgcctcc acggcggtta cgaaggcgtc agtattctcg
     1561 atgacgtggt ccgccggcat cacgagcagc aggcatggtt ctgcgccgct gccgctgttc
     1621 ctgcgcgtga gcagtgcggc cagcgccacc gcgggtgccg tattccgccc ctcgggctcc
     1681 agtacgattg tggcgttgac gccgatgtcg cgcagctgtt cagcaaccag gaagcgatga
     1741 gcttcgttgc agaccaccag ggtggtcgcg gagacgttct gcttaccctt caggcgcagc
     1801 gcggtctgct gcaacatcgt cagctcaccg gtcagcggca acagctgctt cggatacatg
     1861 gccctggagg ccggccagag ccgtgtgcca gacccgccgg aaaggataac tggatgaatg
     1921 acgctcataa atactcttgc ccgccgacca tagggagatt atacgattcg ggcgaatgcg
     1981 gcgacctcgc acggcggggt cctgcaaagc ttcgatcagg ctttgccgac agcctgcaga
     2041 ggcgccggcg aaagctcgga ggcgctgttc accgagcgtc cgcgcccgat cccgtagtac
     2101 gccagtccca gtgcctcgac catcgcgggg tcgtacaggt tgcgaccgtc gaagatcacc
     2161 gcgtcggaaa gctgctgctt caacacggga aaatcgggac tgcgaaactc ctgccattcg
     2221 gtaacgatgg ccagcgcatc ggcgccggta atcgcctcga tagcgttggc acaaagggtc
     2281 aggcccttac tgtccggata caaccttcgg gtttcatcca tggcctcggg gtcataggcg
     2341 cgcaccagcg caccttcctt ccaaagcgcc tccatcagca cgcggctcgg cgcatcgcgc
     2401 atgtcgtcgg t
//
LOCUS       DASUOM010000201         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_128592,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000201 DASUOM010000000
VERSION     DASUOM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128592"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFY27_04190"
     CDS             complement(<1..403)
                     /locus_tag="VFY27_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5764742.1"
                     /translation="MKQFDRKPLARAVSAALTGTALATASLLPAHAQQSGSALEEIVV
                     TAQKREQNLQDVAISVQVLGNQQLEQLNLNNFEDYIEFLPTVSWVQSRPGLSQVYMRG
                     ISSGGDGVHSGSLPSVGVYLDEQPVTTINHVL"
     gene            662..2668
                     /locus_tag="VFY27_04195"
     CDS             662..2668
                     /locus_tag="VFY27_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5764743.1"
                     /translation="MALETQRPVGDTHIELKQALALLQRDPLLAKRKAEEILAVSPDL
                     ADAKQVLGTSLRLLGDPLKALSLIAPIAAVKEPSPDLLHEFGLCLGAAGRGADALEVL
                     RKVLQLDPRHAGAWRSLGDQLAAAGDEKGSEDAYARHLELSTSHPELIDAANYLRQGR
                     LAQAERITREVLKKDPLDVVAIRMLAHVGLRVGQFDDAINLLERCLELAPGFHLARQN
                     YAMALARRQRFADALSEIEQLLAAEPNNPNYLLMKGNFLVQSGQHRIALKLYETFLED
                     YPSQAGAHLNYGHTLKTVGRLEDSIRAYRRSIDLRPTSGEAYWSLATLKTCRFADEDI
                     LRMRSQIAPEDGDADDQAHLAFALGKALEDRHQFDESFSFYEMGNMIRGKQHRHDPRA
                     NIYNTARQIKVLTDEFFRARRGWGSPAPDPIFIVGLPRAGSTLLEQILASHSQVDGTT
                     ELPDIIAMSRKLGNKSRRNPVSDYPEMLTDLSSARVRQLGEDYLASTRVQRRGAPFFI
                     DKMPNNFQHIGLIHLILPHAKIVDARRHPMAGCFSCYKQLFAHGQAFTYDLAHLGYYY
                     RDYLKVMDHWDEVLPGRVHRVQYEKMVADNESEIRRLLEYCGLEFEEQCLRFHETERA
                     VRTPSSEQVRKPIYREGLEQWRNYEKHLEPLKKALGPVLERYPI"
     gene            2780..>3754
                     /locus_tag="VFY27_04200"
     CDS             2780..>3754
                     /locus_tag="VFY27_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764744.1"
                     /translation="MKKAIVIVLTALVNAANAATPVDVARAPYDPDSGSVAIRCGTLV
                     DGVADVPLDDRLIIIRNGRIERVANGATAVPDSLPLLDLSAHTCLPGFIDTHTHLTDR
                     ADETRDLRIYFTRSIEDERRISRSNAEVTLFAGFTTARNVGAYSGWSARDLRDRINAG
                     EIAGPRVQAAGFYLTVPGGGGDLIIPGVDEDDIPHHVRMGVARGPEQFRRKAEEAVAG
                     GADVLKIIASGAVLAYGGIPGAPEMTPEEIAAVVEVGHAAGIKVTAHAHGAQSIKDAI
                     RAGADSIEHASLADDEAIALAAESQVAFSMDVYNGNYIATVGREQGWPE"
BASE COUNT          824 a         1101 c         1131 g          698 t
ORIGIN      
        1 cgaggacatg attgatggtc gtgacaggct gctcgtcgag atacacaccc acgctcggca
       61 gggatccgga atgcacgcca tcaccaccgc tggagatgcc gcgcatatac acctgggaaa
      121 gtccgggacg agactgcacc caggaaacgg tcggcaggaa ttcgatgtag tcttcgaagt
      181 tgttgagatt taactgctcg agctgctggt tgccgagaac ctgtaccgag attgcgacgt
      241 cctgcaggtt ttgctcccgt ttctgcgcgg tgacgacaat ttcctcgagc gcgctgcccg
      301 actgctgtgc atgcgccgga agcagcgatg cggtcgcgag cgccgtaccg gttaacgccg
      361 ctgacacggc ccgagccaga ggcttgcgat cgaattgctt catgttgtta tccccagcag
      421 tatgatgcgt gcgatgtaga ttcgtaatga ccacgaagaa cgcgaaacgt tcccgaccac
      481 gttgttgtca tgcaacgaag gaaggaataa tagcagccac tacgcccagg cagtataacc
      541 gaccgttatg attggctttc gttgcggcag atacgtttca cgatatgaag cctgacttgc
      601 ggatgttgcg aaaaacaaac gtgtttccga agcaggctaa ctgaagcttg ttcgaccaga
      661 tatggcccta gaaactcagc gaccggttgg cgatacccac atcgagctaa agcaggctct
      721 cgcactgttg cagcgggatc ctctgcttgc caaaagaaaa gcagaagaga ttctggcggt
      781 ctccccggat cttgctgacg ccaaacaggt tctcggcacc tcattgcggc tgttgggcga
      841 cccgctcaag gcattgtccc tcatagcccc gatcgcagca gtcaaggaac cttcgccgga
      901 cctgctgcac gagttcgggt tgtgcctggg agcggccgga cgaggcgctg atgccctgga
      961 ggtgcttcga aaggtgttgc agctggaccc gcggcacgcc ggcgcatggc gatcgctcgg
     1021 tgatcaactg gcagccgccg gtgacgagaa aggcagcgag gatgcttacg ccaggcacct
     1081 ggagctctcc acatctcacc cggagctgat agacgccgcg aattatcttc ggcaaggcag
     1141 gctcgcacag gcagagagaa ttacaagaga agttctcaag aaagatccgc tggatgtagt
     1201 cgccatacgg atgctggcac atgtcggtct tcgggtgggt caattcgatg acgcgataaa
     1261 cttactggaa agatgtctcg agcttgctcc cggatttcac cttgcgcggc aaaactacgc
     1321 gatggcgctg gcgcgccggc agaggttcgc cgacgcactg tcggagatag agcagctact
     1381 cgccgccgag ccgaacaatc cgaactacct cttgatgaag ggaaactttc ttgtacagag
     1441 cggacagcat cggattgctc tgaagctcta tgagacattc cttgaagact atccttcaca
     1501 ggccggagcc catctgaact acggacacac attgaaaacc gtggggaggc tcgaggattc
     1561 catccgcgcg tatcgtcgtt ccattgatct tcggccgacg tcgggtgaag cgtactggag
     1621 cctcgcgact ctgaagacat gcaggtttgc ggacgaagat atcctgagga tgcggtcgca
     1681 aattgcgccc gaggatggcg atgccgacga tcaggcccat ctggcgttcg cgctgggaaa
     1741 ggcgctcgag gacagacacc agttcgacga atcgttttcc ttctacgaga tggggaacat
     1801 gatccgtggt aaacaacatc gtcatgatcc gcgagcgaac atctacaata ccgccaggca
     1861 gatcaaggtg cttacggacg agttcttccg tgcccggcgg ggctggggct ctccggcccc
     1921 cgatccgatt ttcatcgttg gcctgccgcg cgcaggctcc acgctcctcg agcaaatcct
     1981 ggcgagccac agccaggtcg atggcacgac cgaactcccg gacatcatcg ccatgtcccg
     2041 caagctcggg aataaatcgc ggagaaaccc ggtgtcggac tacccagaaa tgctcacaga
     2101 cctgtcgtca gcgcgggtcc ggcaactggg cgaagattac cttgcgagca caagggtgca
     2161 acggcgcggc gcaccgttct tcattgacaa aatgcccaac aacttccagc acatcgggct
     2221 tattcacctg atcctgccgc atgcaaagat cgtggacgca cgccgtcatc cgatggccgg
     2281 ctgtttttcc tgctacaagc agctgttcgc acacgggcag gcgttcacct acgaccttgc
     2341 gcatctcggg tactactacc gcgattattt gaaagtgatg gatcactggg acgaagtatt
     2401 accaggtcgt gtacatcgcg tgcagtacga gaaaatggtc gcagacaacg agtcggaaat
     2461 caggaggttg ctcgaatact gcggcctgga attcgaggag caatgcctgc ggtttcatga
     2521 gacggagcgg gctgtgcgga cgccgagttc ggagcaggtc cgcaaaccga tttacaggga
     2581 aggactggaa cagtggcgca attacgaaaa acacctggaa ccgttgaaaa aagccctggg
     2641 gccggtgctc gagcgttatc cgatatagaa gccggatggg aaggcgcgct gcaggagccg
     2701 ccacgagggc gagtgcgaca aggctcagct aactcgcctg catggcaggc tcctacagtt
     2761 acctctatca ggaaatataa tgaaaaaagc catagtcatc gtcctcaccg cgctggtgaa
     2821 cgccgccaac gccgccacac cggtcgacgt tgcgcgggcc ccctacgatc cggacagcgg
     2881 aagcgttgcg attcgctgcg ggaccctggt cgacggcgtc gccgacgtac cgctggatga
     2941 tcggctcatc atcatccgca acggccgcat cgaacgagtg gctaatggag ctacagcggt
     3001 tcccgattca ttgccgcttc tcgatctttc ggcgcacacc tgccttccgg gattcatcga
     3061 cacccacacc cacctgacag accgtgccga cgaaacccgc gatctgcgca tctacttcac
     3121 gcgcagtatc gaagacgagc ggcgcatctc gcggtcgaat gcagaggtca cgctattcgc
     3181 cggttttacg acggcgcgca atgtcggcgc ttattccgga tggtcggcgc gcgacctgcg
     3241 cgaccgcatc aatgccggcg aaatcgccgg gccgcgcgtg caggcggcgg gcttctacct
     3301 caccgttccg ggtggcggcg gcgacctgat catcccgggt gtcgacgagg acgatatccc
     3361 ccaccacgtg cgcatgggcg tggcgcgcgg tccggaacag ttccggcgaa aggccgagga
     3421 agccgtcgct ggcggcgcgg acgtcctcaa gatcatcgct tccggcgcgg tgctcgctta
     3481 cggcggcata cccggggcgc cggaaatgac gccggaagag attgccgcgg tggtggaagt
     3541 gggccacgcg gccggcatca aggtgacggc acacgcgcac ggcgcgcagt ccatcaagga
     3601 cgccatccgc gccggcgccg attccataga gcacgcgtca cttgccgacg acgaagcaat
     3661 cgcactggcg gcggaaagcc aggttgcgtt ctcgatggat gtctacaacg gcaactacat
     3721 cgcaaccgtc ggccgcgaac agggatggcc ggag
//
LOCUS       DASUOM010000202         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_249599,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000202 DASUOM010000000
VERSION     DASUOM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249599"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFY27_04205"
     CDS             complement(<1..942)
                     /locus_tag="VFY27_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5764745.1"
                     /translation="MNSIAHDLRHAARTLLRSPGFTIVAILTIAIGIGANTAIFSVVN
                     RVLLEPLPLPEPDRLVRVYDGSPGIQGTASPPNFVDWRRDNTVFEDMAAYANTSVALT
                     GAGDAKRIFGAAVTEGFFPVLGTVPVLGRPISPADTVAGQQRVVMLGHDLWQRDFGAD
                     PGIVGRTVQLDGRGFTVIGVMPAGFDYPASAELWVPFSVTEEELATQRGAHYLDVIAR
                     LAPGVTVDEASAQMVAISRMLELRYPDTNTGSSASVVGLREALVGDVQPALLILLGAV
                     GFVLLIACANVANLLLARTSGRKRELAVRSALGAGRGH"
     gene            complement(982..>2427)
                     /locus_tag="VFY27_04210"
     CDS             complement(982..>2427)
                     /locus_tag="VFY27_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5764746.1"
                     /translation="LESVILAAGGALLGLVLAMFAVRWMHGQLPEGVASVVDPKIDLR
                     VFFIASLATLATGVVFGLLPALRAGNVNAANVLSGGERSGHAPLVGRLRLGFVVANFA
                     LALALTVGAGLFAKSLLHLESVDPGFRADGVLTATLSFPEASYPDQQALRAAHDALRA
                     KILALPGVSNAGFTHTLPLAGTDNDTSVFIEGVQTDRPDGSAHVWFSIVTPGFLDGLQ
                     IRPLHGRVFTEQDHGGRADNVIVNDAFVRQYLQGKEPLGTRITSRSAAEGEWLTVVGV
                     VDDVRFFGMDAQQTPAMYLPMHKYPARRMYLTLRGGGDPLLLAGPLRKAVASVDPTLA
                     LGEVRPMTALVDTSLRPARSIAFLIGSFASAALFIAMIGVYGSISYAVSQRRREFGVR
                     MALGATGGKVLKMVLRQGLQMALAGILIGIALTAASGRGIRSLLYQVEPMDPYVVVGV
                     ALLLLAVAVAATFIPAWRAARTQPVRVLREE"
BASE COUNT          479 a          824 c          740 g          384 t
ORIGIN      
        1 gtgcccccgc cccgcgccaa gtgcgctgcg caccgcgagt tcgcgcttcc tgccggaggt
       61 gcgcgcaagc agcaggttcg cgacgttggc gcacgcgatc aatagcacga agccgaccgc
      121 gccgagcagg atcagcagtg ccggctggac atcaccgacg agagcctcgc gcaatccgac
      181 caccgatgcg ctggaaccgg tattcgtatc cggataccga agctcgagca tgcgcgagat
      241 tgccaccatc tgcgccgacg cttcgtccac ggtgaccccg ggagcgagac gcgcgatgac
      301 gtccaggtag tgagcaccac gctgtgtcgc gagttcttcc tcagtgacgg agaacggcac
      361 ccacagttcg gcactggctg gatagtcgaa tcctgccggc atcacaccta tgaccgtgaa
      421 gccccgccca tcgagctgca ccgtccgccc gacaattccg gggtcggcgc cgaaatcccg
      481 ttgccacagg tcatggccca gcatcacgac ccgctgctgg ccggccaccg tgtcggccgg
      541 tgaaatcggc cggccaagca cgggtaccgt gccaagcacc gggaagaagc cttcggtcac
      601 cgcggcgccg aagatacgct ttgcatcacc cgcgccggtc agcgcaacag aggtattggc
      661 gtaagcagcc atgtcctcga acacggtgtt gtcgcgccgc cagtccacga agttcggcgg
      721 cgaagccgtg ccctggatgc cgggactgcc gtcgtatacc cgcacgagcc ggtcgggctc
      781 cggcagggga agcggttcca gcaacaccct gttcacgaca ctgaaaatgg ccgtgttcgc
      841 gccaataccg attgctattg tgagtatggc tacgatggta aagccgggac tccggagcag
      901 ggttctcgcg gcgtgacgta aatcgtgcgc gatggagttc atcggatttt gccgtcgcac
      961 atttgcctgt cgctcatcgc tctattcctc gcgcaacacg cgcaccggct gcgtcctggc
     1021 agcgcgccac gcagggatga aggttgccgc cacggcgacg gcgagcagca gcagcgccac
     1081 cccgacgacg acgtacgggt ccatgggctc aacctggtaa agcagcgagc ggataccgcg
     1141 tcccgaagcc gccgtcagtg cgatcccgat gaggatcccc gcgagtgcca tctggagtcc
     1201 ctgtcgcaga accattttca gtaccttgcc gccagtcgcg ccgagcgcca tgcgcacgcc
     1261 gaactcgcgg cggcgctgtg atacggcata agagatgctg ccgtagacac cgatcatggc
     1321 aatgaacagc gcggcgcttg cgaaactgcc gatcaggaag gcgatggaac gcgccggccg
     1381 cagcgacgtg tctacgagcg ccgtcatagg cctgacctcg cccagggcaa gcgtcggatc
     1441 caccgatgcg actgctttcc tcaggggccc tgcgagcaac agcggatcac cgccaccgcg
     1501 aagcgtcagg tacatgcggc gagccggata tttgtgcatc ggcaggtaca tcgccggcgt
     1561 ctgttgggcg tccatgccga agaagcgcac atcgtcgacg acacccacca ccgtcagcca
     1621 ctcaccttcg gcggcactac gtgacgtaat gcgagttccc aacggctcct ttccctggag
     1681 gtactggcgg acgaacgcat cgttcacgat gacattgtcc gccctgccgc cgtgatcctg
     1741 ctcggtaaac acccgcccat gcagcggacg aatttgcagg ccatccagga agccgggcgt
     1801 cacgatgctg aaccacacat gcgcgcttcc gtcgggccga tccgtttgca cgccctcgat
     1861 gaacacactc gtgtcgttgt cggtccccgc gagaggcagg gtatgtgtga agccggcgtt
     1921 gctcacccct ggcaaggcga gaatcttcgc ccgcagtgca tcgtgcgcag cgcgcaacgc
     1981 ctgctggtcc ggatagcttg cttcggggaa actgagtgtc gccgtgagca ccccgtctgc
     2041 gcggaacccc gggtcaacgg attccaggtg cagcaggctt ttcgcaaaca aacctgcgcc
     2101 aaccgtcaat gcgagcgcga gcgcaaagtt cgcaaccaca aagcctaacc gtaaccggcc
     2161 gacaagcggc gcatgaccgg atcgttcgcc gccgctcaag acgttcgccg cgttgacgtt
     2221 gccggcgcgc agggcaggca gcagcccgaa aacgaccccg gtagcaaggg ttgccaggct
     2281 cgctatgaaa aaaacgcgca ggtcgatctt cgggtccaca acgctcgcaa ccccttccgg
     2341 caactggccg tgcatccagc gcaccgcgaa catcgccaga acgagaccca gcagcgcgcc
     2401 gcctgccgca aggatcacgc tctccag
//
LOCUS       DASUOM010000203         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_254091,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000203 DASUOM010000000
VERSION     DASUOM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254091"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1337)
                     /gene="purD"
                     /locus_tag="VFY27_04215"
     CDS             complement(60..1337)
                     /gene="purD"
                     /locus_tag="VFY27_04215"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5764747.1"
                     /translation="MKVLVVGGGGREHALAWKCAQSPKVTEVLVAPGNAGTALEPRLR
                     NIAVAADDIDELLVVAQSEAVGLTIVGPETPLVAGIVDRFRAHGLPCFGPTAKAAQLE
                     GSKSFSKAFLRRHNIPTAAYESFTELEPALAYIRGRGAPVVIKADGLAAGKGVVVAST
                     LEEAEQAAMQMLAGAFGNAGRKIVIEEYLEGEEASFIVITDGTDVLPLATSQDHKARD
                     DGDAGPNTGGMGAYSPAPLVTPGLERRIMEEIIFPTLAGLRAEGLDYLGFLYAGIMVA
                     SDGSPRVLEFNCRFGDPETQPIMTRLQSDLVEICEATLAGELQGVSAEWDSRAALGVV
                     MAAGGYPGPYRKGDPISGLPDTRTGAVKIFHAGTALQDDAVVTDGGRVLCVVGLGSTV
                     AEAQETAYRQVRRIHWADAYYRTDIGYKALNVN"
     gene            complement(1345..2880)
                     /gene="purH"
                     /locus_tag="VFY27_04220"
     CDS             complement(1345..2880)
                     /gene="purH"
                     /locus_tag="VFY27_04220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5764748.1"
                     /translation="MRRALLSVSEKSGLVQFARGLAELGVEILSTGGTFRVLSDAGVE
                     VVEVSARTGFPEIMGGRVKTLHPVIHGGLLGRRGTDDDAMVRHGIQPIDLLAVNLYPF
                     EATIARAGSTLDDAIENIDIGGPAMIRAASKNHAGVAVVVEPADYEAVLQAIRSGELS
                     QEYRRQLATKAFAHTAAYDTAIADYLNKVCNGASPGERFLYSGRRIEMLRYGENPHQQ
                     AAFFRDRDARPGTLACARQLQGKALSYNNIADSDAALACVRQFDEPACVIVKHANPCG
                     VAVDDSIGRAYERARSTDPTSAFGGIIAFNRGLDGNTARAIIERQFVEVIIAPGVDDA
                     ALEALRQKQNVRVLVTGEAAPGGDYDLKKVSGGLLVQSADLGRIRPEDLKVVTERAPD
                     ATELRDLMFAWQVAKYVKSNAIVFCRDRMTIGIGAGQMSRVYSTRIAALKAADEGFEI
                     RGSAMASDAFFPFRDGIDAAAEAGAAAVIQPGGSLRDDEVIAAADEHGLAMVFTGMRH
                     FRH"
     gene            complement(2939..3217)
                     /gene="fis"
                     /locus_tag="VFY27_04225"
     CDS             complement(2939..3217)
                     /gene="fis"
                     /locus_tag="VFY27_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEX5764749.1"
                     /translation="MVKKKTKKDVELRTAGKKPLRKHAEEALDRYFASLNGDRPGDLY
                     ELVLGEVEEPLFKAVMDYTQGNQSQAAGILGINRGTLRKKLKSYALIR"
     gene            complement(3305..4303)
                     /gene="dusB"
                     /locus_tag="VFY27_04230"
     CDS             complement(3305..4303)
                     /gene="dusB"
                     /locus_tag="VFY27_04230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX5764750.1"
                     /translation="MIIGPFLLRSPFLLAPMAGITDAPFRRLCRRFGAGMTTSEMTTA
                     DIRLWHTDKSSRRLDLDLDCEPRVVQIAGSEPGQLALAAKHCVDRGAQIIDINMGCPA
                     KKVCSKLAGSSLLRDEKLVATILDAVVKSVPVPVTLKIRTGWDPQHRNGVTIARIAES
                     CGIQALAVHGRTRECRFRGHAEYETIAAIKNSVSLPVIANGDITTFEKSLEVLRVTGA
                     DALMIGRGAQGKPWVFREFKYRYEKNDSKNDSTMPVENNFVRDIILDHLSDLYQFYGE
                     TTGVRVARKHLIWYCAGLTNTQTFRAQVVRVDSASEQLRLTREFFSNGSADEHLAA"
     gene            complement(4317..>5052)
                     /locus_tag="VFY27_04235"
     CDS             complement(4317..>5052)
                     /locus_tag="VFY27_04235"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX5764751.1"
                     /translation="TIIMEGDFVTNALEAEFALGPRPTGKQGGSAALERRSRTPRSGE
                     PDSPDDEDDILDSYVKSTDGMRGGRRRTDPPSYRVIAGVAALALVLAAQVVHAYREAL
                     ATYPVFDRTVGSVYRLLGEPLNPDWNIKEWQFEATSGRTDEAGGVLTISSRLANSSSR
                     ALPYPLLHVSLTDRWEEIIGSKILEPTDYLDSNANPAASVPPGERFTAMVKVQAPSPE
                     ATGFKLNVCYREPGNRVRCATEDFKD"
BASE COUNT          909 a         1596 c         1482 g         1065 t
ORIGIN      
        1 tgccgaaatc cggcatcgct tgcgcaatcg caactcttgc tgcttccgtc gtgagcatgt
       61 cagttaacgt tgagcgcctt gtatccgata tccgttcggt aatacgcatc ggcccagtga
      121 atgcgccgca cctggcgata agccgtttcc tgcgcttcgg ccactgtgct gcccagcccg
      181 acgacgcaga gtacccgtcc cccgtctgtc accacggcat catcctgcag tgccgtgccg
      241 gcgtgaaaga ttttcaccgc ccctgtcctt gtatcgggca agccgctgat gggatcacct
      301 ttccggtacg ggccggggta gccgccggcc gccattacca cgcccagtgc ggcgcgtgaa
      361 tcccattcgg cgctgacgcc ttgaagctcg ccagcgagcg ttgcttcgca gatttcgaca
      421 aggtcggact gaagccgcgt catgatcggc tgtgtctcgg ggtcgccgaa gcgacaattg
      481 aactccagta ccctgggaga gccgtcgctc gcgaccatga tacccgcata gagaaacccg
      541 aggtaatcga ggccttcggc acgcagcccg gcaagcgtcg gaaatatgat ctcttccatg
      601 atgcgccgtt cgagcccagg cgttacgagc ggcgcgggcg aataggcgcc cataccgccg
      661 gtgttcggcc ccgcatcgcc gtcatcgcga gccttgtgat cctgtgatgt cgccagcggc
      721 aacacgtcgg taccgtctgt gatgacgatg aaactcgctt cttcgccctc gaggtattcc
      781 tctatgacga tttttcgccc cgcgttgccg aacgcgccgg ccagcatttg catcgcagcc
      841 tgttctgctt cttccaaggt cgacgcaacg acgacaccct tgccggcggc aagtccgtcg
      901 gccttgatga cgacgggagc tcccctgccg cggatgtaag cgagcgccgg ctcgagttcg
      961 gtgaagcttt cgtacgccgc tgtaggaatg ttgtggcgcc ggaggaaagc cttgctgaag
     1021 gacttcgatc cctccagttg tgcggctttc gctgtcggcc cgaagcaggg gagaccgtga
     1081 gcgcgaaagc ggtcgacgat accggcgacc agcggagttt ccgggccgac gatcgtaagg
     1141 cctaccgctt ccgattgcgc gacgacgagc agctcgtcga tgtcgtccgc tgccacagcg
     1201 atattgcgca gcctgggctc gagcgcggta ccggcatttc caggagccac cagcacttca
     1261 gtcactttgg gtgactgggc gcacttccag gcgagcgcat gctcccgacc gccaccgcct
     1321 accaccagaa ccttcatctg taccctagtg cctgaagtga cgcatacccg tgaacaccat
     1381 tgcgagcccg tgctcatctg cggccgcaat tacttcgtcg tcccgcaacg agccgcccgg
     1441 ctggattacc gccgctgcgc ctgcctctgc cgccgcgtca atgccgtcgc ggaaggggaa
     1501 aaacgcatcc gacgccatgg cggagcccct gatttcgaag ccttcgtccg ccgccttcag
     1561 agccgctatg cgggtgctgt agacgcggct catctggcca gcgccgatcc cgatcgtcat
     1621 ccggtccctg caaaagacga tcgcgttgga tttgacgtac ttggcaactt gccacgcaaa
     1681 catgaggtcg cgcagttccg tggcatccgg tgcccgttcg gtgacaactt tgagatcttc
     1741 cgggcggatg cgcccgaggt cggcgctttg cacgagcagt ccgcctgaga cttttttcag
     1801 gtcgtaatcg ccaccgggcg ccgcttcgcc agtcacaagc acccggacgt tctgcttctg
     1861 ccggagcgcc tccagcgcgg catcgtcaac gcccggtgca atgataacct cgacaaactg
     1921 gcgctcgata atggcgcgcg cggtgttgcc gtcgagcccg cggttgaacg caatgatgcc
     1981 gccgaacgcc gacgttggat cggtagacct tgcccgctcg tacgcccgcc cgatgctgtc
     2041 gtcaacggcg acaccgcagg gattcgcatg cttgacgatg acgcaggcgg gttcgtcgaa
     2101 ctggcgaacg caggcaagcg ccgcatcgct gtccgcgatg ttgttgtacg acagcgcctt
     2161 gccctgcagc tggcgcgcgc aggccagggt acccggccgc gcgtcccgat cgcgaaagaa
     2221 ggcggcctgc tgatgcggat tctcgccata acgcagcatc tcgatccgac ggcctgaata
     2281 aaggaagcgc tcacccgggg atgctccgtt acaaacctta ttgagatagt ctgcgatcgc
     2341 ggtatcgtag gccgcggtat gggcaaaggc cttcgtagca agttgtcttc gatactcctg
     2401 tgacaactcg cctgatcgga tcgcctgcag aaccgcctcg tagtcggccg gctcgacgac
     2461 gaccgcaact cccgcgtgat tcttcgacgc ggcgcggatc atcgcgggtc cgccgatgtc
     2521 gatgttttct attgcgtcat cgagggtgga ccctgcccgt gcgatggttg cctcgaaggg
     2581 gtagaggttg acggcgagca ggtcgatcgg ctggatcccg tgccggacca tcgcgtcatc
     2641 gtccgtaccg cggcgtccaa gcaggccgcc gtggatcacg gggtgcaagg tcttgacccg
     2701 gccgcccatg atctccggga agcccgtcct ggcggagacc tccacgacct cgaccccggc
     2761 gtcgctcaat acccggaacg tgccgccggt ggaaagaatt tcgacgccca gttccgcgag
     2821 gccccgggca aactggacca gcccgctctt ttcggagacg ctcaggagcg cccggcgcac
     2881 ggttatctct gcaggtgctg gcggctttga cttcgacaag agcaaattca ggccgcggtc
     2941 agcgtatcag ggcataggat tttagttttt ttctgagggt gccacggttg attccgagta
     3001 tgccggcggc ctggctctgg tttccttgcg tgtagtccat aacggctttg aacaatggtt
     3061 cttcgacctc gcccaggaca agttcataaa ggtcgcccgg acgatcgccg ttgagactcg
     3121 cgaaataccg gtccagtgct tcttccgcat gcttgcgaag tggctttttg cctgcggtcc
     3181 tcaactccac atcttttttt gtttttttct ttaccactgt ctgatgattc cccctgtcgt
     3241 ttcctcctgc cgtttcccca ggagtggaac gattcgcgaa atacgttttc cgttgcacat
     3301 attttcaggc agccagatgt tcgtctgcag agccattgct gaaaaattcc cgcgtcaatc
     3361 gcagctgctc ggaagcactg tcgacacgca ccacctgtgc ccgaaacgtt tgtgtatttg
     3421 tcaaaccggc gcaataccag atgaggtgtt tgcgagccac ccgaacgcca gttgtttccc
     3481 cataaaactg gtacaggtcg ctaaggtggt cgaggataat atcacggacg aagttatttt
     3541 ctaccggcat tgttgaatca ttttttgaat catttttttc gtaacgatat ttgaattcac
     3601 ggaacaccca cggctttcct tgcgcccctc tcccaatcat caaggcgtca gcaccggtta
     3661 cacggagaac ttcaagcgac ttctcgaatg tggtgatatc tccgttggca atgaccggaa
     3721 gcgaaaccga attttttatt gcagcgatgg tttcgtactc ggcatgaccc cggaaccggc
     3781 actcccgtgt ccggccatgt accgcaagcg cctgtatgcc acagctttcg gcgatgcgag
     3841 cgatcgtgac gccgttcctg tgctgcggat cccatccggt ccggatcttc agtgtcaccg
     3901 gaacaggaac ggacttgacg actgcgtcga gaatcgttgc taccagcttt tcatcgcgca
     3961 ataacgatga acccgccagc ttgctgcaga ctttcttcgc ggggcaaccc atgttgatgt
     4021 cgatgatctg cgcgccgcga tcgacacaat gctttgcagc cagggcgagc tgccccggtt
     4081 cgctgccggc aatctgcacc actctcggct cgcagtcgag atcgagatcc aggcgcctgc
     4141 tggatttgtc cgtgtgccac aggcggatgt cggccgtggt catctccgat gttgtcatgc
     4201 ccgcgccgaa acggcggcaa agcctgcgga agggcgcgtc cgtaatgccc gccatcgggg
     4261 ccaggagaaa cgggctccgc agaagaaacg gtccgataat cacggcaggt caggcactag
     4321 tctttgaagt cttccgtggc gcagcgcacc cggttcccgg gctcccggta gcagacattc
     4381 aacttgaatc cggtggcttc gggcgacgga gcctgcactt tgaccatcgc cgtaaagcgc
     4441 tctcccggcg gcacgctggc agcgggattg gcgttcgaat cgaggtaatc cgtgggctcg
     4501 aggattttgc tgccgatgat ctcctcccac cggtcggtca gcgagacgtg caggagcggg
     4561 taaggcaacg ccctcgagga gctgttcgca aggcgtgacg aaatagtaag cacgccgcct
     4621 gcctcgtccg tccgcccgct cgtcgcctcg aactgccatt cctttatatt ccagtcaggg
     4681 ttcagcggct cgcccagcag gcggtatacc gagcctaccg tcctgtcgaa caccgggtat
     4741 gtggccagcg cttcgcgata ggcatgcact acctgcgctg caagcacgag cgcaagtgcg
     4801 gcaacgcctg ctatcaccct gtaactgggc ggatccgtcc tgcggcgtcc gccgcgcatg
     4861 ccgtcggtgc tcttgacgta gctgtcgaga atgtcgtctt catcgtcagg cgaatcgggc
     4921 tcgccggacc tgggtgtgcg ggagcgtcgt tcgagcgccg cgctcccgcc ttgtttgccg
     4981 gtggggcgag gacccagcgc gaactccgcc tccagggcgt tcgtgacgaa gtcaccttcc
     5041 atgatgatcg tt
//
LOCUS       DASUOM010000204         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1569835,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000204 DASUOM010000000
VERSION     DASUOM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569835"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFY27_04240"
     CDS             <1..279
                     /locus_tag="VFY27_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5764752.1"
                     /translation="PIASYYASVSRMTVENERFFPEHVMTRDEALTSYTINNAIAAFE
                     EDIKGTLTPGKLADIVVLSQDILNVAEEKIPATKVDMTIVGGEIRYQR"
     gene            286..2247
                     /locus_tag="VFY27_04245"
     CDS             286..2247
                     /locus_tag="VFY27_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEX5764753.1"
                     /translation="MPGKSARPLLAAMFLASLPAAAASAAPETPSDEGWPQYGRDAGG
                     TRHSPLTQINASNLEQLEIAWQFRTGELGQDARQGEKLTFEATPVLWRGTLYFSTGFG
                     TAFAIDAASGRQVWRFDSKVPRDIGFAEITSRGVTVWEDPERDSSSACKGRVYLGNIV
                     GRLYALDALTGEPCRDFGDRGLVDLHRDARPREVGQYAITSPPAMAGDRIIVGSAIGD
                     NSAVGLERGIVRALDARSGETLWSWDPVPTDPDDPAYRTWKSGVEESGGGNAWPPLSV
                     DVANELVFVPTGAPSTDFYGGLREGDNAYANSLVALSIETGEVQWFRQLVHHDVWDYD
                     LPAQPLLVEFEKDGREHSAVVQTTKMGMVFVFDRLTGAPIYPIEERPVPQGGIPGEKL
                     SPTQPFSSLPPLVSHDAITEDDAWGLLYFDRRGCRKKISALRSEGIYTPATIGGTLMN
                     PGYAGGSNWGGLAWDPESQTVIATVLELPMWVRLTPREEAQRQMDSETYDWEGYTEMD
                     GTPYVLNRGMLLSALEVPCTKPPWGKLVGLNLGSGEIEWERPIGTIQDIAPAPVPNLE
                     LGVPLIGGAITTASGLIFVAATFDDYLRAFDVATGEELWKGRLPASGQATPMTYAIDD
                     RQYVVIAAGGHGGAGTTRGDYVVAFALKE"
BASE COUNT          451 a          703 c          746 g          422 t
ORIGIN      
        1 ccgatcgcat cctactatgc atccgtctca cgcatgaccg tggagaacga gcgcttcttc
       61 ccggagcacg taatgacgcg cgatgaagcg ttaacgagct acacgatcaa caacgcgatc
      121 gctgcgttcg aggaagatat aaagggtaca ttgactcccg gaaagctcgc tgacatcgtc
      181 gtgctttcgc aggacatcct caatgtcgcg gaagaaaaaa tcccggcgac gaaagtggat
      241 atgaccatcg tgggcggcga gattcgctat cagcggtgag ccaacatgcc aggcaaaagc
      301 gctcgtccgt tactggctgc gatgttcctt gcatcgctgc cggccgctgc ggcgagcgct
      361 gctcctgaaa ccccctctga tgagggctgg ccacaatacg gccgtgacgc cggaggcaca
      421 cgccactcac cgctgacaca gatcaatgcc tccaatctcg agcaactcga gatcgcctgg
      481 cagttccgca ccggagagct cgggcaggac gcgcgccagg gtgagaagct gacgttcgag
      541 gcgacgccag tgctctggcg cggcactctt tatttcagta ccggctttgg caccgcattc
      601 gcgatcgatg ccgcgagcgg acggcaagtc tggcgttttg attcgaaggt gccccgggat
      661 atcggcttcg cggaaatcac ctcgcgcggc gtcaccgtct gggaagaccc ggagcgagac
      721 agcagttccg cctgcaaagg ccgcgtctac ctcggcaaca ttgttggcag gctgtacgcg
      781 ctcgatgcgc ttaccggtga gccctgtcgc gacttcggcg accggggctt agtggatttg
      841 caccgcgacg cgcggccgcg ggaagtcggg cagtacgcca taacgtcgcc tcccgcgatg
      901 gccggcgaca ggatcattgt cggctccgcg atcggcgaca acagcgcggt aggactggaa
      961 cgcggcatcg tgcgggcgct ggacgcgagg agcggcgaga cattgtggag ctgggaccct
     1021 gtccccacag atcccgacga tccggcttat cgaacatgga agagcggcgt ggaggagtcg
     1081 ggtggcggaa acgcatggcc gccgctgtcg gtcgacgtcg ccaatgaact ggtcttcgtg
     1141 ccgacgggtg cgccctcgac cgatttttat ggcggtctgc gcgagggaga caacgcttac
     1201 gcgaactcgt tggtcgcact ttccatcgaa accggggaag ttcagtggtt tcgacagctc
     1261 gtgcaccacg acgtctggga ctacgacctg ccggcgcaac cgttgctggt ggaatttgaa
     1321 aaggacgggc gagagcactc cgccgtcgtg caaaccacga agatgggcat ggtgttcgtc
     1381 ttcgaccggt tgaccggagc gccgatatat ccgatcgaag aacggcctgt tccgcaggga
     1441 ggaattccgg gcgagaagct ttcgccgacg caaccgttct cgtcgttgcc gccgttggta
     1501 agtcatgacg ccataaccga ggatgatgct tgggggcttt tgtacttcga caggcggggc
     1561 tgccggaaga aaatttcggc gcttcgctcg gaaggaattt atacgcctgc gaccatcggc
     1621 ggcacgctca tgaatcccgg ctatgcaggc ggttctaact ggggcggcct tgcgtgggac
     1681 ccggagtcgc agacggtcat cgcgacggtg ctggaactgc cgatgtgggt gcgccttacg
     1741 ccccgcgaag aggcacaacg gcagatggat tcggagacgt acgactggga aggttacacg
     1801 gaaatggacg gcacgcctta cgtgctgaac cgcggcatgc tgttgtcggc gctcgaggtt
     1861 ccatgcacca agccgccgtg gggcaaactg gtcgggctga acttgggtag cggcgagatc
     1921 gagtgggaac ggccgatcgg cactatccag gacatcgctc ctgccccggt gccgaacctc
     1981 gagctgggcg tgccgcttat tggcggcgcc atcactacgg cttccggcct aatcttcgtg
     2041 gcagccacct tcgacgacta cctgcgcgcc ttcgatgtcg cgactggcga agaactctgg
     2101 aaaggccgcc ttcccgccag cggccaggca acacccatga cctatgccat cgacgaccgc
     2161 caatacgtgg tcatcgccgc cggcggccac ggcggcgccg gcacgacccg cggcgactac
     2221 gtcgtcgcgt tcgcgctcaa ggagtagcgt ggcctgccgg cgacgctcgt ttcctttaca
     2281 atcaacctca agcgcccgta gctcagctgg atagagtact gc
//
LOCUS       DASUOM010000205         7443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_607940,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000205 DASUOM010000000
VERSION     DASUOM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607940"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VFY27_04250"
     CDS             complement(<1..1640)
                     /locus_tag="VFY27_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764754.1"
                     /translation="MASQTRMMCTSVAVVATLLIGVPSVSSAQDEDSEFGPQITTAPP
                     TSAGRRGAGEGDAYTPAPGAKDLKSVLFNWAWHMGMLRSTEEYDLLMSLVYQGTGTVQ
                     VNGQPCNVTMYRSDISYQKSGERIRITGTRPNGQSCSTIEVLSGAYTWNEDIMGAELV
                     AGQGKATPMPATVEERMIRLWANPQGAWKAAMFGTQDTNAPQMAARPQRVPADVMNVH
                     KTSVAWSGNQPVVTFPIPGVPTATATATLDAKFMAESVVVKNGADTYEFTYGNYKDWN
                     NPLHPAEAFYTGKITEKKNGAVIRDITTTMTETGQMYVVMPVPASVKAAIKPTHQPPN
                     WTLTPREPPAPVAAAATPRRADGKPDLSGNWNRAINPVSGSGARRCGPTQVKGGGINP
                     EYGCTGGGDNFWIEYAWISPSRFGILGHPNYKPEFWDQIQELDMWTNKYDPVMTCKPL
                     GLPRHGTPTRIIHTDKDLVFFYPANADYGGGNNEYRTIPIDGRERSALDLSLTTFYGV
                     SLGTWEGDTLVIDSINFDDSTWLGRGGFFHSTSMHIVE"
     gene            complement(1662..2057)
                     /locus_tag="VFY27_04255"
     CDS             complement(1662..2057)
                     /locus_tag="VFY27_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX5764755.1"
                     /translation="MITRALGCVVAFGCLATSASLQAHHSTAGVYDPGKEVKVTGALA
                     KLQFVNPHGSITVTVKNADGTTTDWTFTTGSATALASQGITKVGPKALKIGEEVTVLG
                     TPARNGSPLGGLKSLTRADGTVLGNVGGN"
     gene            2712..2933
                     /locus_tag="VFY27_04260"
     CDS             2712..2933
                     /locus_tag="VFY27_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764756.1"
                     /translation="MPTSPAKPRLVPCHFTVEDLQLIEWSCREKAQRARKDAKSDTTP
                     SSIEAFTSTATKYEALASRIQRLKELGTR"
     gene            complement(3221..5167)
                     /locus_tag="VFY27_04265"
     CDS             complement(3221..5167)
                     /locus_tag="VFY27_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5764757.1"
                     /translation="MKDRNSLAAVALMLAAILSEESVIPQEAAPPFDVQEKTIAEIQQ
                     AIINREITTRGVVEAYLRRIQAYNGTCVNEPQGILGPITPIAHARQINALSTLNLRPA
                     TREAWMFDARKARSLTDTVDDAEGMPDALETAAEQDQQFARTGQLVGPLHGVVLAIKD
                     QYDTFDMRTTSGADADYANDRPPDDATFVKRLRDAGAIILAKANLAEYAVDGARSSFG
                     GTFCNPYDTEREPGMSSAGSATAVAANLVTCAIAEETVVSVRWPASVNSTVGLAPTQE
                     LVSRDGMMGSGLVMRVGPICRTVEDTAKILDAYAGYDPKDELTAFSVGRKPPQPYSSF
                     ADTRRLDGVRIGVLREYMRRSLLTMADEESVALIERALVDLRSLGAVIVDPGAEGELF
                     TPCIARYAPELLNSAFARQFPQLFPSDAPAAGGADISALLDLQQDPSRLLQHLSLRTL
                     SGGGLGSAGEGKYMLNRYLRERGDANIRSNADLITKARFYQDPNFPDRKQARAQAERA
                     AVLDTAVRLQGRFALQTILLQCMQEQRLDALVSPMSTVPPRKLTSPREPASNGRTPIG
                     WSLIGQQGFPVITVPAGFTKNVWDRVADGDDTRLVGPVEAALPVGVDFIARPFDEPLL
                     LRIASAYEAATKHRKAPAEFGAVP"
     gene            complement(5607..7208)
                     /locus_tag="VFY27_04270"
     CDS             complement(5607..7208)
                     /locus_tag="VFY27_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764758.1"
                     /translation="MQFFEALFLLALAVGIARLLAGPIHPRLAYSTIAVVGLCLMVAG
                     IVSEGFRWQMVPAYVGFGVLVIALLKKSKPRLVWRTLGALPLFLLMSASALLSQALPV
                     FSLPEPSGPYGVGTFEYSITDNARKERYAPTRNRELYVEVWYPAEKSALRDFPVRTLF
                     QELYEGDYTRQSLLFGYLRYIPTHSHVHPPVARPDDGPFPVLLFNHALELGFTSQNQL
                     LMEHLASHGYVIFSIAHPYQSSKVNLGNAGTITVASEFPTDMDLTRPELHQGVVGTIY
                     EASHDLDEISVVKEALSPLAERYFALDDGDRRAFLTQAVAMEEFHAYRDFISEDLLED
                     YFVYDYSVQNSLTQYWVEDIQFVASTLNDLRPPVVGFSESIDTSGFGVFGMSYGGGTA
                     GEFCKIDSRCKAGVNLDGTQFGRHWNHAVSAPFLMLYHEEHQGGNDFAYLPPTREFWD
                     YGIKGATHFDFTDFSYAWPLFKTLGYTGPIDGLRMVDIVNIVVLNFFDHSLRGKPLAG
                     ELFTDIPEIIVRQHSIDAQSRIDEN"
BASE COUNT         1440 a         2277 c         2238 g         1488 t
ORIGIN      
        1 ctctcgacga tatgcatgct ggtggagtgg aagaagccgc ctcgtcccaa ccacgttgaa
       61 tcgtcgaaat tgatcgagtc gatgacgagc gtgtcgcctt cccaggttcc taggctcacc
      121 ccatagaacg tcgtgaggct caaatccagc gcgctgcgct cgcggccgtc gatggggata
      181 gtccggtact cattgttgcc gccgccgtaa tcggcattgg ccggataaaa gaagacgagg
      241 tccttatccg tgtggatgat gcgcgtcggc gtgccgtgcc gcggcagtcc cagcggcttg
      301 cacgtcatga cgggatcgta tttgttcgtc cacatatcga gctcttggat ctggtcccag
      361 aactcgggtt tgtaattcgg gtggccgagg atcccgaacc gcgacggcga aatccacgcg
      421 tattcgatcc agaagttgtc tcccccgccg gtgcagccat actcggggtt gatgccgcca
      481 cccttcacct gcgttgggcc acagcggcgg gctccactac ccgaaactgg attgatcgcg
      541 cgattccagt tgccggaaag gtccggcttc ccatcggcgc ggcgcggcgt ggcagcggct
      601 gcaaccggcg cgggcggttc gcgcggcgtt agcgtccagt tcgggggctg atgcgtcggc
      661 ttgatcgccg ccttcacgct cgccggcacg ggcatcacga catacatctg tcccgtttcc
      721 gtcatcgtcg tcgtaatgtc gcggatgacg gcgccgtttt tcttctccgt gatcttgccg
      781 gtatagaacg cctcggcagg atggagcggg ttgttccagt ccttatagtt gccgtacgtg
      841 aactcgtacg tgtccgcgcc gttcttgacc acgacgctct cggccatgaa tttcgcgtcg
      901 agcgtggcgg tggccgttgc agtggggacg cccgggatcg ggaacgtcac caccggttgg
      961 ttgcccgacc atgcgaccga cgttttgtgt acgttcatca catcggcggg cacccgttgc
     1021 ggtctcgctg ccatctgcgg cgcattcgtg tcctgagtgc cgaacatcgc ggccttccat
     1081 gcaccctgcg ggttcgccca cagacgaatc atccgctcct cgacggtggc cggcatcggt
     1141 gtggctttgc cttggcccgc gacgagctca gcgcccatga tgtcttcgtt ccaggtgtac
     1201 gccccgctga gcacttcgat cgtgctgcag ctctgcccgt tgggccgagt gccggtgatg
     1261 cggatccgct cccccgactt ctggtagctg atgtcggagc ggtacattgt cacgttgcag
     1321 ggttggccgt tcacttggac cgtgcccgtg ccctgataga cgagcgacat gagcagatcg
     1381 tattcctcgg tgctgcgcag catgcccatg tgccaggccc agttgaacag cactgacttc
     1441 aaatccttgg cgccgggggc aggcgtgtag gcgtcgcctt cgcctgcgcc gcgacgtcct
     1501 gcgctcgtgg gcggcgccgt ggtgatctgg gggccgaatt ccgagtcttc gtcttgagcg
     1561 ctagatacgg acggcacgcc gatcaggagc gtcgcaacga cggcgacact cgtgcacatc
     1621 attctcgttt gagaagccat agtgatctcc ccccggttca ctcaattgcc gccgacgttg
     1681 ccgaggaccg tgccatccgc ccgcgtcagc gacttgaggc ctcccaaggg gctcccgttc
     1741 cgtgccgggg tgcccaacac ggtcacctct tcgccgatct tgagcgcctt cgggccgacc
     1801 ttggtgatcc cttgtgaggc gagcgccgtg gccgaaccgg tcgtgaaggt ccagtcggtc
     1861 gtcgtgccat cggcgttctt caccgtcacc gtgatcgagc cgtgcggatt cacgaactgc
     1921 agcttggcca gcgcgcccgt gactttcacc tctttccccg ggtcgtagac accagcggtg
     1981 gagtgatgcg cctggagcga cgcactggtc gccagacacc cgaatgcgac gacgcatccc
     2041 aaagctcgtg taatcatttg tgcctctcca aaaggttgtg atcggcaagt gccatcatat
     2101 gtcatcggaa aaaccggata accgcccgac gagcgtgcag cacgcgcgag ctcaaatcaa
     2161 gggtcgaggg gcgacgatag cagcgaaggc gacgagcgta agttgctgtc tggagtagtg
     2221 gccgtgctgg gctcgaacca ccgaccgatg gattacgagc ggttggttgt gccgacccaa
     2281 ggacagccat catcagcaaa ccaagcgctg aggaagcgcc gatccccccg aggtctttcg
     2341 cgcccagcgt ccacgccgcg atggcgccgt ggtagctgtg aaccaaacag acactttggt
     2401 tacgaccgat ttgaacggac ggctagcctc cgagctcctt gtgatcgcga tccggtccag
     2461 agtcggcttg gtcgtcatcg agatctgctg gttcgacgtc cggaatctgg ggctctgacc
     2521 cgcctcctgt actacctctc cgaggatcgc ggcgcttctt gtaatcttca ttctgtagcg
     2581 tcggcatagt ttcgctcctt gagcatgtgt tgacatcaaa gacgcgcggt cggatgcaag
     2641 acatgcgcca tagtcgcggc ctttcttttc agtgtagggc ccgattggcc gagatttggc
     2701 atactggatc aatgcccact tcacccgcga aaccgagact cgtcccgtgc cacttcacgg
     2761 tcgaagatct acagctcatc gaatggtcgt gccgggaaaa ggcgcaacgc gcgcgcaagg
     2821 atgccaagag tgacacgaca ccaagctcga tagaggcatt cacgtccacc gcgacgaagt
     2881 acgaagccct cgctagtcgt atccagcgcc taaaggaact cggcacgcgt tgatctcgcc
     2941 cgtcgccgag ccgctgagag ctcacccgcg ggcggtcgag gcgctgatcg acccgagaac
     3001 cgccttcggg cggctctttc ttcttccgaa cgctgtcggc catggcgcct agcactgacc
     3061 cggcgctggc tccaaagacg aggcccatac gcggcggtcc ctcgctcgcg ccaagtggta
     3121 ttcgcagtca tcgctttgcg gggactgcgg ccgagttcca tgccgacggc tgcgcagata
     3181 ctcgtcgagc cgggaatgag ccgggacgtt cgacttccgc ttaagggacg gcaccgaact
     3241 cggctggcgc cttgcggtgc ttagtcgccg cttcatatgc cgatgcgatg cgaagcaaca
     3301 gcggttcatc gaacgggcgc gcgatgaaat cgacgccgac cggaagagcc gcttccacag
     3361 gaccgacgag gcgcgtatcg tcgccgtcgg cgacccgatc ccacacattc ttcgtgaagc
     3421 cggccggcac cgtgatcacc gggaatcctt gctggccgat cagcgaccac ccgatcggcg
     3481 tgcgtccgtt ggacgccggc tcgcgcggcg acgtcagctt gcgcggcggc acggtggaca
     3541 tcggcgacac gagcgcatcg agacgctgct cctgcatgca ctgcaacaag atcgtctgca
     3601 gcgcgaaacg cccctgcaga cgaaccgcgg tatccagaac ggctgcgcgc tcggcctgcg
     3661 cacgggcctg cttgcgatcc gggaagttcg ggtcctggta gaaccgcgct ttggtgatca
     3721 gatccgcatt gcttctgata ttcgcatcgc cgcgctcgcg cagatagcga ttgagcatgt
     3781 acttaccttc gcccgcggag cctaacccac caccgctcaa cgtccgcagt gacagatgct
     3841 gcagcaggcg cgaagggtcc tgctgcagat cgagcagtgc cgaaatgtcg gcgccgcctg
     3901 cggctggcgc gtcggagggg aacagctgcg gaaactgccg tgcgaatgcc gaattcagaa
     3961 gctcgggcgc atatcgagcg atgcagggcg tgaacagctc tccctcggcg ccgggatcga
     4021 cgatgaccgc gcctagcgaa cgtaggtcga ctagcgcgcg ctcgatgagg gcgacgcttt
     4081 cttcgtcagc catcgtgagc agcgatcgcc gcatatactc gcgcagcacg ccgatgcgga
     4141 cgccatcgag acggcgtgta tcggcaaagc tcgagtacgg ttgcggaggc ttgcggccaa
     4201 cgctgaaggc ggtcaactcg tccttggggt cgtatccggc gtaggcatcg aggattttgg
     4261 ccgtgtcctc gacagtccgg cagatcggcc cgactcgcat aacgaggccc gatcccatca
     4321 ttccgtctcg gctcacgagc tcttgcgtcg gcgccaggcc gacagtgcta ttcaccgacg
     4381 caggccaacg caccgagacg accgtttctt cagcgattgc acacgttacg aggttcgccg
     4441 cgaccgccgt cgccgagccc gcgctcgaca ttccgggctc gcgttcagtg tcatacgggt
     4501 tgcagaacgt gccgccgaac gagctgcgag cgccgtcgac cgcgtactcg gcaagattcg
     4561 ccttcgccaa gatgatcgca ccggcgtcgc gcaatcgctt cacgaacgtt gcatcgtcgg
     4621 gcggccggtc gttggcgtag tcggcgtccg cgccggacgt ggtgcgcatg tcgaacgtgt
     4681 cgtattggtc cttgatcgcg aggacgactc cgtgtagcgg accgacgagt tgtccggtgc
     4741 gcgcgaactg ctgatcctgc tccgccgctg tctcgagcgc gtcaggcatg ccctcagcat
     4801 catccaccgt gtcggttagg ctgcgcgcct tgcgcgcgtc gaacatccat gcctcgcgtg
     4861 tcgcaggccg aagattcaga gtcgagagcg cgttgatctg tcgcgcgtgc gcgatgggtg
     4921 tgatgggacc gagaataccc tgcggctcgt tgacgcatgt gccgttgtac gcctgaatgc
     4981 gcctcagata cgcttcaacg acgccccgcg tcgtgatctc tctgttgatg attgcttgct
     5041 ggatctccgc gatcgtcttt tcctggacgt cgaacggggg cgcagcttcc tgtgggatca
     5101 cactctcctc gctcagaatt gcggctagca tcagcgcaac cgccgcaaga gaatttcggt
     5161 ccttcatcgc gccctcaact cgtgaccctt cagcccatta tccgcttccg ggcgtctgac
     5221 ctggataccc ggttgagatg ctcgaccacg gccggcgttg cgtcgatcca gcccgacagc
     5281 tcgtcataca cttcgcagcg gcgctcgccg gtctccttgt tcgtgaagaa gatcgcgggc
     5341 ccttcgcgat gaccgttatc ggatgggttt tggggataac cgcacgccag gctgctaaag
     5401 cctgcgtaag tctttgagga aattggtggg ccgtgcttgg gctcgaacca gcgaccaatg
     5461 gatgaaaagt agcagagatt gacgagccag caagatctgg caaaaccgaa tcaaagcgtt
     5521 aagggccttc gtctcggggg caagtcgtgc ccgctgacgc tcgctcagtt atttcggtag
     5581 cagtctagta gcagttgcta ccccagctaa ttctcatcaa tccgagactg cgcatcgatc
     5641 gaatgctgcc ggacgatgat ttccgggata tcggtaaaca actccccggc gagcggcttg
     5701 cccctcaagc tgtggtcaaa gaagttcagt acaacgatat tcacgatgtc gaccatcctc
     5761 agaccgtcga tggggcccgt gtagccgagc gtcttgaaga gcggccatgc atagctgaaa
     5821 tcggtgaaat cgaagtgtgt cgcgcccttg atgccgtaat cccagaactc acgcgtgggc
     5881 ggtaagtagg cgaaatcgtt ccctccctga tgctcctcgt gatagagcat gagaaaaggt
     5941 gccgaaacgg cgtgattcca atgacgaccg aattgcgtgc catccagatt gacaccagcc
     6001 ttgcagcggc tatcgatctt gcagaattct cccgcggtcc cgccgccata cgacatgccg
     6061 aaaacaccga acccgctcgt gtcgatcgac tcggagaacc caacgaccgg cggccgcaag
     6121 tcattaagcg tgctggcgac gaactgaata tcctcgaccc agtactgagt caacgagttc
     6181 tgcaccgagt agtcgtagac gaaatagtct tcgagcagat cttcggaaat gaagtctcgg
     6241 taggcgtgaa actcttccat cgcaaccgcc tgagttagaa aagctctcct gtcaccgtcg
     6301 tccagagcaa agtatctttc agccagcggc gagagagctt ccttcaccac cgagatttcg
     6361 tccagatcat gggacgcttc gtaaatcgtg cctaccacgc cttgatggag ctccgggcga
     6421 gtgagatcca tgtcagttgg aaactcacta gcgactgtga tcgttcctgc gttaccgagg
     6481 tttaccttcg aagattgata tgggtgagcg atgctaaaga tcacataccc gtggcttgcc
     6541 aaatgctcca tcaacaattg attttgggag gtgaagccaa gctcgagggc gtgattgaaa
     6601 agcaataccg ggaacggccc atcatccggt ctcgcaacag gtgggtgcac atgagagtgc
     6661 gtcgggatgt atctgagata cccgaacagc aagctttgcc gcgtgtagtc accctcatag
     6721 agctcctgaa acagcgtccg gacagggaag tcgcggagcg cactcttttc cgccggatac
     6781 cagacctcga cgtaaagctc acggttgcgt gtaggcgcgt atcgttcctt cctcgcgtta
     6841 tccgtaatgg aatactcgaa ggttcctaca ccgtacggac ctgagggttc tggtaacgaa
     6901 aagacaggca atgcttggct caagagggcc gacgcgctca tcagcaagaa cagcggaagt
     6961 gcgccgagcg tacgccacac tagtcttggc ttggactttt tcaacaacgc tatcacgagg
     7021 acgccgaaac cgacgtatgc tggaaccatc tgccaacgaa agccttcgct gacgataccc
     7081 gccaccatca agcacagccc gacgacagcg atcgtcgaat aggccaatcg agggtgaatg
     7141 ggtccggcaa gcagtctggc tatgccaact gcgagcgcta acaaaaacaa ggcttcgaag
     7201 aactgcaacg gatgtctcct tgcagtgaac tacgaacgaa ccgcgaggaa tttacaggcg
     7261 gcgagcacat cgagacggcg ccaacaccga tcgtgcgttc accaatcgac tggcgaattt
     7321 tggtagcaaa ccgggcccgt aggggccgaa agtaccggag ctatcggttg aattggtggg
     7381 ccgtgctggg ctcgaaccag cgaccagtgg attaaaaagg aggcagggac gaacgcacgg
     7441 tcc
//
LOCUS       DASUOM010000206         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1040020,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000206 DASUOM010000000
VERSION     DASUOM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040020"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /gene="gmk"
                     /locus_tag="VFY27_04275"
     CDS             complement(<1..165)
                     /gene="gmk"
                     /locus_tag="VFY27_04275"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Essential for recycling GMP and indirectly, cGMP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5764759.1"
                     /translation="MSSSVTSGRRARLFVIAAPSGAGKTTLVRALLERNPSLSFSISY
                     TTRPKRKSEEE"
     gene            complement(155..1021)
                     /locus_tag="VFY27_04280"
     CDS             complement(155..1021)
                     /locus_tag="VFY27_04280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5764760.1"
                     /translation="MLHSMTGFARHTVESEFGVLTCELKTVNHRYFDVQFRLPEELRP
                     KETDLRQLVGSAVRRGKVDCSLHYRRAPGKRAELSLNQALVEQIIRRAGELQAAFPAL
                     ANLNAIDLLRWPGVIEEPEVETEPLYEEAVAVITVALGDLSAMRRSEGQRIAEMIDSR
                     CREIMGIAASVRERMPEVLASARARQKERLDRLALDADPARLEAELALVAQKLDVDEE
                     VDRLESHVSEIRSTIGSPEAVGRRLDFLMQELNREANTLGSKSADTATTRAAVELKVL
                     IEQMREQIQNVE"
     gene            1308..2066
                     /locus_tag="VFY27_04285"
     CDS             1308..2066
                     /locus_tag="VFY27_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX5764761.1"
                     /translation="MLSIMRIEYAKVSAVGDRQDNQDRAAVIVAEEAALMLVFDGMGG
                     HSDGARAAEVGLRTIQDMFTAARLPIFDPQGFLYTALARAHDEVVRIGMDLPVDFRPR
                     ATCAICLIQENGSFWGHIGDSRIYQVREGRVLARSRDHSHVEVLIQEGAITEEEAQDH
                     PMRNFVECCIGGDAPVPDMTITRKRRLNPGDVLLACSDGLWSGINDEEIARLATRADE
                     DLAENLKALTVRALTANAPYSDNTTGTLLRWLGG"
     gene            2269..3015
                     /gene="rph"
                     /locus_tag="VFY27_04290"
     CDS             2269..3015
                     /gene="rph"
                     /locus_tag="VFY27_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5764762.1"
                     /translation="MRPSGRAHDQMRPVRFSTNFTKYAEGSVLAEFGDTRVLCTASVE
                     GRVPPWMRGAGRGWVTGEYGMLPRATHLRSGREAARGRQGGRTMEIQRLIGRSLRAAI
                     DLDALGERTITLDCDVLQADGGTRTAAISGAYVALQLAVGALLKTRVLKSNPVHGQIA
                     AVSVGIYRGKPVLDLDYAEDAEAETDMNVVMNEAGRLIEVQGTAEGHAFSIEELGQML
                     DLAQSGVVQILAAQVAAQGGEGAPAVGSRC"
     gene            3062..3706
                     /gene="rdgB"
                     /locus_tag="VFY27_04295"
     CDS             3062..3706
                     /gene="rdgB"
                     /locus_tag="VFY27_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5764763.1"
                     /translation="MIPTVSPSTRTVVIASGNQGKLAEIRQLLAPLELDVRPQSDFTI
                     QPVAETGATFLENALLKARHASAIAGLPAIGDDSGLEVAALGGGPGVRSSRFAGEAAT
                     DEENIEKLLKALRNLPEGERKANFTCVAVYVRSPDDPQPLVAEGRWDGSILSQRRGGG
                     GFGYDPVFLDPVAGRTAAEMNGEEKNSASHRGQAFRALSALIAESVDARQGDRT"
BASE COUNT          680 a         1173 c         1248 g          699 t
ORIGIN      
        1 ttcttcttcg ctcttccgct tcgggcgcgt cgtgtacgaa atagagaagc ttaaggaggg
       61 atttctttcg agcaacgccc tgaccagcgt cgtcttcccg gccccggagg gagcggcgat
      121 gacgaacagc cttgcgcggc ggccggacgt tacgctactc gacattctgt atctgctcgc
      181 gcatctgttc gatcaggact ttgagttcta cggcggcgcg tgtcgtggcc gtgtcggccg
      241 acttcgagcc gagcgtgttc gcttcgcggt tcagttcctg catgaggaaa tccaggcgcc
      301 tgcctaccgc ttccgggctg ccgattgtcg agcgtatctc gctcacgtga ctctccagcc
      361 ggtccacctc ttcgtcgacg tccagcttct gcgccaccag cgcgagttcc gcctcgagcc
      421 tggccgggtc tgcatcgagt gcaagcctgt ccaggcgctc tttttgccgt gcgcgcgcgg
      481 acgcgagcac ttccggcata cgctcgcgca cggaggcggc gatacccata atttcccggc
      541 agcgcgagtc gatcatctcg gcgatgcgct gcccctcgct ccggcgcatt gccgacaggt
      601 caccgagtgc cacagtgatg accgccactg cttcctcata caggggttcc gtctcgacct
      661 cgggctcctc gatgacgccg ggccagcgca agaggtcaat cgcattgagg ttggcaaggg
      721 ccgggaacgc cgcctgcagt tcgccggccc gccgtatgat ctgttcgaca agcgcctggt
      781 tcagcgaaag ctccgccctt ttccccggcg cccggcggta atgcagtgag cagtcgacct
      841 tgccgcgccg aacggcgctg ccgacaagct gccgcaggtc cgtttccttc ggccgcagct
      901 cctccggcag gcggaactgc acgtcaaagt agcggtgatt gacggtcttg agttcgcagg
      961 taaggacgcc gaactccgat tcgacggtat gccgcgcgaa ccctgtcatg ctgtgtaaca
     1021 tcaaaatccc cgaaggccct gcgatcccaa ggaccgccag gaccccgctc attttttgtt
     1081 gacgcggtca ctgacaggcg ggtttcatgc attcatactg ccgctcctca gtgaccgcga
     1141 ctggtgaacg gatccctaga gtctgcgaag caggagtaat gtctgccgcg cagctaaagg
     1201 ctgcgataat tctaacccga agcgccattg ccatggcgag cagcttcgtg tcagtgtgat
     1261 tgcccgcctg atgcgaccga agttgcgccg caaacaaacc acagtagatg ttgagcatca
     1321 tgcgcatcga gtatgcaaag gtttccgccg tcggagaccg ccaggacaac caggaccgtg
     1381 ccgccgtgat cgtggcggaa gaggccgccc tgatgctggt gtttgacggc atgggcgggc
     1441 attcggacgg cgcgcgcgcg gcggaggtcg gcctcaggac gatccaggac atgttcacgg
     1501 cagcacgcct gccgattttc gacccgcagg gctttttgta tacggccctc gcgcgagcgc
     1561 acgatgaggt agtgcgcatc ggcatggacc ttccggtcga cttccgtccg cgtgcgacct
     1621 gcgccatctg cctgatccag gagaacgggt cgttctgggg gcacattgga gacagccgga
     1681 tctaccaggt gcgggaaggg cgcgtgctgg caaggtccag ggatcacagt cacgtcgagg
     1741 tcctgatcca ggaaggggcg attaccgagg aggaggccca ggatcacccg atgcggaatt
     1801 tcgtggaatg ctgcatagga ggcgatgcgc cggtgccgga catgacgatc acgcgcaaga
     1861 gacgcctgaa cccgggggat gtcctgctgg cttgcagcga tggcctgtgg tccggcatca
     1921 acgacgagga aattgcgcgg cttgcgacgc gcgcggacga agacctcgcg gaaaacctga
     1981 aggcactgac ggtgcgtgcg ttgacggcta acgcaccgta tagcgacaac accaccggta
     2041 cgctcttgcg ctggttgggc gggtagcccc gtaaccgcgg cgcggcgccc acgatctggc
     2101 cgtggcgcaa gagcccgcca tcctggcgag ggcgccgagg ctcatcgaac acgcccgcca
     2161 tccgcgttcg caaccccacc cggattctgt cttggcgttc atgtcggccc ggactccggc
     2221 gatgcctcgt tgctttatca tgcggataac caggcgagga gagcacgtat gcgaccgagc
     2281 ggccgcgcgc acgaccagat gaggcccgtg cgtttcagca cgaatttcac caaatatgcc
     2341 gagggcagcg tcctggccga attcggggat accagggttc tttgcaccgc gtccgtcgag
     2401 ggccgtgtac ctccatggat gaggggtgca gggcgcggct gggttaccgg cgagtacggc
     2461 atgctgccgc gggcaacgca cctgcgctcc ggaagggagg cggctcgcgg gcggcaggga
     2521 gggcgtacca tggagatcca gcgcctcatc gggcgctcgc ttcgggccgc gatcgacctg
     2581 gatgcacttg gagagcgcac gatcacgctg gattgtgatg tgctgcaggc ggatggcggt
     2641 acgcgcaccg cggcgatcag cggagcgtac gttgcgctgc agctcgcggt gggtgcgctc
     2701 ctgaagacga gggtgctcaa gtcgaacccg gtgcacggac agatcgcggc agtttcggtc
     2761 ggcatctatc gcggcaagcc ggttctcgac cttgattatg ccgaggacgc ggaagcagag
     2821 acggacatga acgtcgtcat gaacgaagcc ggccggctga tcgaggtgca gggcaccgcg
     2881 gagggccatg ccttcagcat tgaggagttg ggtcaaatgc tcgatctcgc ccaatcgggc
     2941 gtggtgcaga tacttgccgc acaggttgcc gcgcagggtg gcgagggtgc gcctgccgtc
     3001 gggtcgcgtt gctgaatcgg ctcgccttat ttcaaaccct gcaggctaac cggcaaccac
     3061 gatgataccg acagtcagtc ccagcacccg tacggtcgtt attgcgagcg ggaaccaggg
     3121 caaactcgcg gaaatccggc agttgctggc accactggaa ctcgacgtcc gcccgcaatc
     3181 ggacttcacc atccagcccg tggcggagac cggcgccacc ttccttgaaa atgcgctctt
     3241 aaaggcgcgc catgcttcgg cgattgcggg actgcccgct attggcgatg actcgggact
     3301 cgaggtcgcc gcgctcggtg gcggtcccgg cgtgcgttcg tcccgctttg ccggcgaggc
     3361 ggcaacggat gaggaaaaca ttgaaaagct gctcaaggcg ctccgcaacc tgccagaagg
     3421 agagcgaaag gccaacttca cgtgcgttgc ggtgtacgta cggtcgccag acgacccgca
     3481 gccacttgtc gccgagggca ggtgggacgg cagcattctt tcgcagcgcc gcggcggtgg
     3541 cggctttggc tacgacccgg ttttcctgga tccggttgcg ggtaggacgg cggcagagat
     3601 gaacggggaa gagaagaact cggcgagtca ccgtggacag gctttcaggg ctttatccgc
     3661 actgatcgca gaatccgtcg acgcgcgcca gggagaccgg acgtgacccc gccattgtcg
     3721 ttttacctcc atctgccgtg gtgcgtccgc aagtgtcctt attgcgactt caattcccac
     3781 acggcgagcg gggcggctga
//
LOCUS       DASUOM010000207         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1240334,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000207 DASUOM010000000
VERSION     DASUOM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240334"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..638)
                     /locus_tag="VFY27_04300"
     CDS             complement(54..638)
                     /locus_tag="VFY27_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX5764764.1"
                     /translation="MDKLTRETLFSLEKYAEVRNHFRAEVMAHKRDRRLALGTNATLY
                     FEDRLTMQYQVQEMLRIERIFEAAGIGEELDAYNPLIPDGNNWKATFMVEFPDVAERR
                     AMLQKLVGIEEHVWMQVDEFEKIRPIADQDLERDTEQKTSAVHFLQFDLPQEQIDALK
                     DGASLAAGIDHAAYQVDVRPVPDNVRASLLKDLD"
     gene            complement(659..2002)
                     /locus_tag="VFY27_04305"
     CDS             complement(659..2002)
                     /locus_tag="VFY27_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5764765.1"
                     /translation="MRDPKDNREGSLDAPTRHNIAWQTEEYYDEPALMHELERVFDIC
                     HGCRRCFNLCNSFPTLFDAIDESETMELDSVPKEVYWKVVDHCYLCDMCYMSKCPYVP
                     PHPWNVDFPHLMLRAKATKFKTKPVKKRDRILSSTDNLGRFAGIPIVVQGVNTSNNSK
                     AGRALLEKTLGVHRDAPIPVWHSKSARKRLRRHRKENGEAAVATATTRGKVVLFVTCY
                     GNRNMPTLAEDLAAVYEHNGIPLALAEKEACCGMPKLELGDFESVARLKDKNIPLLAS
                     WVDRGWDIVTPIPSCTLMFKQELPLMFPHDPDVIKVRDAMYDPFEYLMLRHRDGKLRT
                     DFKQSLGKVAYQVPCHLRVQNIGLKTRELLMLAPDTQVEAIERCSGHDGTYAVKKEFH
                     EISKKIARPVVNKINKGDAEHFVSDCPMAAEQIGQDLDELGPQHPMGLLRRAYGI"
     gene            complement(2052..>2296)
                     /locus_tag="VFY27_04310"
     CDS             complement(2052..>2296)
                     /locus_tag="VFY27_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5764766.1"
                     /translation="LEYLEEVGDPATGLPIGATSANLAAAIAGETHEYTDMYPGMAKT
                     ARDENFDEIADWFETLAKAERSHANRFQKALDTLDK"
BASE COUNT          406 a          700 c          668 g          522 t
ORIGIN      
        1 gtgcgatgat gcttgatcac actcgcccgc atggcgggct cctaccgatg gaactagtcc
       61 aggtctttta gcagcgacgc gcgaacgttg tccggaaccg ggcgaacgtc aacctggtag
      121 gcagcgtgat cgatgccggc ggccagagag gcgccgtctt tcagggcgtc gatctgttcc
      181 tgtggcaaat cgaattgcag gaaatgcacc gcggacgttt tctgctcggt atcccgctcc
      241 agatcctggt ctgcgattgg ccttatcttt tcaaactcat cgacctgcat ccacacgtgc
      301 tcctcgatac cgacgagctt ctgcagcatc gcccgccgtt cggcgacgtc ggggaactcc
      361 accatgaacg tcgctttcca gttgttgcca tcaggaatca gcggattgta ggcatccagt
      421 tcctcgccga taccggcggc ttcgaagatg cgctcgatgc gcagcatttc ctgcacctgg
      481 tactgcatcg tcagccggtc ctcgaaatac aacgtggcgt tggtgccgag cgcgagccgc
      541 cggtcgcgct tgtgcgccat cacctcggcg cgaaagtgat tccgcacctc ggcgtacttc
      601 tccaggctaa agagtgtctc gcgcgtcagc ttgtccatag gttcataacc tttttctgtc
      661 agataccgta ggcgcggcgc aggagcccca tcgggtgttg cggcccgagc tcgtcgagat
      721 cctggccaat ctgctccgcg gccatggggc aatcgctcac aaaatgctcc gcgtctccct
      781 tgttgatctt gttgaccacc gggcgtgcga tcttcttcga gatttcgtgg aactccttct
      841 tgacagcgta ggttccgtca tgtccggagc agcgctcgat cgcctcgacc tgcgtgtccg
      901 gagccagcat cagcagctcc cgtgtcttca acccgatgtt ctgtacccgc aggtgacacg
      961 gaacctggta tgcgacctta ccgagcgatt gcttgaagtc cgtgcgcagc ttcccgtccc
     1021 tgtggcgcag catcaggtac tcgaacgggt cgtacattgc atcgcgtacc ttgatgacat
     1081 cgggatcgtg agggaacatc agcgggagtt cctgcttgaa catcaatgtg caggaaggaa
     1141 tcggcgtcac gatatcccag ccgcgatcca cccaggaggc gagcagcggg atgttcttgt
     1201 ccttgaggcg cgcaaccgac tcgaaatcgc caagctcgag cttcggcatg ccgcagcagg
     1261 cttccttttc ggcgagcgcc agcggaattc cattgtgctc gtagacggcg gcaaggtctt
     1321 cggccaaagt tggcatgttg cggttgccgt aacaggtcac gaacagcacc accttgccgc
     1381 gcgttgttgc cgtagctacg gctgcctcac cgttttcctt gcgatgcctg cgcaagcgtt
     1441 tgcgcgccga tttcgaatgc cacaccggga ttggcgcatc gcggtgcacg cccagcgttt
     1501 tttcgagaag tgcgcgccct gctttgctgt tgttgctcgt gttgacgccc tgcaccacga
     1561 tcggtatgcc ggcaaaacgc ccgaggttat ccgttgaact caggatccgg tctcgcttct
     1621 tcaccggctt cgttttgaac ttcgttgcct tcgcgcgcag catcaggtgc ggaaaatcca
     1681 cgttccaggg atgcggcggc acgtacgggc atttcgacat gtagcacatg tcgcacagat
     1741 agcaatggtc gacgaccttc cagtagacct ccttcggcac cgagtccagt tccatggttt
     1801 cggactcatc gatggcgtcg aagagcgtgg gaaaagagtt gcacaggttg aagcaacggc
     1861 ggcagccgtg gcagatgtcg aatacccgct cgagctcatg catcagcgca ggctcgtcgt
     1921 aatactcctc ggtctgccag gcaatgttgt gccgcgtcgg agcgtcgagg ctgccctcgc
     1981 gattgtcttt tgggtcgcgc attggacacc aggaccggca gccctggaac ggccgccggt
     2041 ctcctgcgag attacttgtc cagcgtgtcc agtgctttct ggaaacgatt ggcgtgcgaa
     2101 cgctcggcct tggccagcgt ttcgaaccag tcggctattt cgtcgaagtt ctcgtcacgt
     2161 gccgtcttcg ccatgccggg gtacatatcg gtgtactcat gcgtttcgcc ggcgattgcc
     2221 gctgcaagat tcgccgaggt agcgcctatc ggcaggcccg tagccgggtc gcccacttcc
     2281 tcgagatact cgagat
//
LOCUS       DASUOM010000208         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1136937,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000208 DASUOM010000000
VERSION     DASUOM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136937"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFY27_04315"
     CDS             <1..919
                     /locus_tag="VFY27_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764767.1"
                     /translation="LSVVHGSGATVTPIRSDAGTTARFSDVAGMEPLKKLLRLQIIEP
                     FRNPGLFQKFKKKAGGGVLLYGPPGCGKTLTARAIAGECNAHFQSVGISDVLNMWIGE
                     SERNLAAIFEQARARRPCVLFFDELDALAYSRSKAASSAARTVVNEFLAQLDGFNADN
                     EGVLVLAATNMPWDVDPAMKRPGRFARQVFVPPPDEAARSEMLRGKLRDVPTEDVDVA
                     LVTSRTEHFSGADIDGLIDAAKDRVLAEILDTGRNRGVRTSDLLEALDDASPSTMDWL
                     RTARNLVKFSGADSSYRDVEKYLKKARLS"
     gene            1061..2251
                     /locus_tag="VFY27_04320"
     CDS             1061..2251
                     /locus_tag="VFY27_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5764768.1"
                     /translation="MEIKRQQIDTWRAQTPGCHERVHLNNAGAALPPASVIDAMSAHL
                     SLEARIGGYEAEAEAKSGIADCYTRVGDLIGTSAHNVAIVENATVALAQALSSFDFTP
                     GDRIVTTRLDYPSNQLMYLSLVRRCGVEIVRAEDLPEGGVDPASVARLADHPRCRLVA
                     LTWVPTNSGTVQDAHAVGAICESLGIPYIVDACQVVGQLPVDVRSLRCDFLAATGRKF
                     LRGPRGIGFLYVSDRALQRGAYPLYVDMRGAKWIASNDFQLADDARRFENWEFACALV
                     LGLGEAARYALEAGTAAMHRTQGLARALRERLATVGNLELMDRGTETSAIVTVATGRD
                     SSTLVKQLRTLRVNTSASCREDGVIDMDEKQEASVLRLSPHYYNTEAELDVAVAALAD
                     LLGD"
BASE COUNT          461 a          706 c          751 g          440 t
ORIGIN      
        1 gctgagcgtc gttcacggga gcggcgcgac ggtcactccg attcgaagcg acgccggcac
       61 gacggcccgc ttcagcgacg tcgccggtat ggagcccttg aagaaacttc tgcggctgca
      121 gatcatcgag ccatttcgca atccaggcct gtttcagaag ttcaagaaaa aggccggcgg
      181 tggagtcctg ctttacggac cgccaggatg cggcaaaacg ctgaccgccc gcgcgattgc
      241 aggggaatgc aacgctcact tccagtctgt cggcataagc gatgtcttga acatgtggat
      301 tggcgaaagc gaacgcaatc ttgcggccat cttcgagcag gcacgcgccc gccggccttg
      361 cgtgctgttc ttcgacgaac tcgacgccct cgcctattcg cgttcgaagg ctgcatcgag
      421 cgccgcccgc acagtggtca acgagttcct tgcgcaactc gacggcttca atgccgacaa
      481 cgaaggtgtg ctggtgcttg ccgcaacaaa catgccgtgg gacgtcgacc ctgccatgaa
      541 aaggcctggc cggtttgcac ggcaggtctt cgtgccgccg cccgacgagg cggcgcgctc
      601 ggaaatgctg cgcgggaagc tacgggatgt gccaacagag gacgtcgacg tcgcactggt
      661 cacctcacgc acggagcact tttcgggagc tgacatcgac ggattgattg atgcggcgaa
      721 ggacagggtg ctggcggaga tcctggatac cggcaggaat cgcggagtca ggacgtccga
      781 tctgctcgaa gcactggacg acgcgtcgcc cagcaccatg gactggctca gaacggcaag
      841 gaacctggtg aagttttccg gcgccgacag cagttacagg gatgtagaga agtacctgaa
      901 gaaggccagg ctttcctgat tgtaaagaga cgtcttccac atggaagcca ggcgagacgg
      961 agcatcctgt accctcttcg atttcggtcg atttccatca ccggtgttcg cctctcgcgg
     1021 ctaaacaaga cgcgggttcg gcatatacaa tctggtttac atggagataa agcgccaaca
     1081 aattgacacc tggcgcgctc aaacccccgg gtgccatgag cgtgtgcacc tgaacaatgc
     1141 gggcgcggcg ctaccccccg cgagcgtcat tgatgccatg agcgcgcatt tgtctcttga
     1201 agcgcggatt ggtggttacg aggccgaggc agaggcaaag agcgggatcg ctgactgcta
     1261 cacgcgtgtg ggagacctga tcggcacatc ggctcacaac gtggcgatcg tcgagaacgc
     1321 gacggtggca cttgcgcagg cgctgtcgag cttcgacttt accccgggtg atcgcatcgt
     1381 tacgacgcgg ctggactacc cttccaacca gctgatgtac ctgtctttag tccgccgctg
     1441 cggcgtggag atcgtgcgcg ccgaggatct tcccgagggt ggcgtcgatc ccgcaagcgt
     1501 cgcgcgcctc gcagatcatc cgcgttgtcg cctcgtagca ttgacctggg taccgacgaa
     1561 ctccggaacg gtgcaggatg ctcacgcggt cggcgccatc tgcgagtcgc tgggtatccc
     1621 ttacatcgtc gatgcatgcc aggtcgtcgg gcagctgccg gttgacgtcc ggtccctgcg
     1681 ttgtgatttc ctggcggcta ccggtcgcaa gttcctgcgc gggccgcgcg gaatcggatt
     1741 cctctacgtc agcgatcgcg ccttgcagcg cggcgcatat ccgctgtacg tcgacatgcg
     1801 tggcgcgaaa tggatcgcgt cgaatgactt tcaactggcc gatgacgccc gccgcttcga
     1861 gaattgggaa ttcgcatgtg ccctcgtcct gggcctcggc gaagctgcgc gctatgcgct
     1921 cgaggcaggt acggcggcaa tgcaccggac gcagggactg gcgcgggccc tgcgcgagcg
     1981 gctggccact gtcgggaacc tggaactgat ggatcgcggc acggaaacga gcgccatcgt
     2041 gacggtggct accggacgcg actcatccac actcgtgaag caactccgaa cgctcagggt
     2101 caatacgagc gcctcatgca gggaggacgg tgtaatcgac atggacgaga agcaggaggc
     2161 atcggttctg aggctgtcac ctcactacta caacacggaa gcagaactgg atgttgctgt
     2221 agccgcgctc gccgatctac tgggagattg acgcccagct cacgcggtta tcgggaggcg
     2281 ttctgccata aagcaacttg cgccgcagtc cagcgccgac atttcggcgc tgctcgccgt
     2341 gccccggtga cgcgaatg
//
LOCUS       DASUOM010000209         5070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1330915,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000209 DASUOM010000000
VERSION     DASUOM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330915"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFY27_04325"
     CDS             <1..467
                     /locus_tag="VFY27_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX5764769.1"
                     /translation="EFYSVAVANNMQQADTGTKMIHIGRNSRSTIVSKGISAGRAQNA
                     YRGLVRVMPQAENARNHTQCDSLLLGKDCGAHTFPYVEVRNPTAKVEHEATTSKISAD
                     QLFYCRQRGISEEDAVNMIVNGFCKEVFRELPMEFAVEAQNLLNVSLEGAVG"
     gene            497..1249
                     /gene="sufC"
                     /locus_tag="VFY27_04330"
     CDS             497..1249
                     /gene="sufC"
                     /locus_tag="VFY27_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5764770.1"
                     /translation="MLEIKNLKARIGETGILTGLSLTVKPGEVHAIMGPNGSGKSTLA
                     LVLAGHEDYVVTEGSVTYMGKDLLGLDPEERARIGVFLGFQYPVEIAGVNNAYLLKAA
                     LNAKRRAQGEPEVDAFEFLKLAREKMALLDMDQALLNRGVNEGFSGGEKKRNEILQMA
                     VLEPRLAILDETDSGLDIDALKAVAKGVNSLRDAERAIVLVTHYQRLLDYVEPDFVHV
                     LSGGKIVRSGDKSLAHMLEEKGYDWVREAANA"
     gene            1246..2571
                     /gene="sufD"
                     /locus_tag="VFY27_04335"
     CDS             1246..2571
                     /gene="sufD"
                     /locus_tag="VFY27_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5764771.1"
                     /translation="MSTSTIDTALLERAVARLPADSLARARKEALARFAKTGFPTTRH
                     EDWRYTNLAPAIGVSNLWLREAAAGAIEEGIGEGVQRYADWLNTQIDAWWIVIANGVV
                     QEESLMRLRERAPGALEARLLASAGESKCLEAAAMAADEPMTRFNTALLRDALRITVH
                     AGREIERPIGLLMIDSAGSTSTVSQSRVFIETGANSRAEFIEYHASLGEHGHFANVVT
                     ELGIGPNAAVDFVRIQDRDAAHFQVGRLHAAMGRDSRLRHAAFDVGGALIRNDIAVDI
                     AEPGAAVELLGLYLAAGRQHIDNHTRVDHRTGPANSSEEYRGILTDHSRCVFNGKAIV
                     HKGADGTDAQQANHNLLLSDKAEIDTKPELEIYADDVKCSHGATVGQLDKTAMFYLRS
                     RGLSRDEAAQVLTRAFAARIIAKSPVPASRAHIEGLIDRRLQALVGDVL"
     gene            2568..3830
                     /locus_tag="VFY27_04340"
     CDS             2568..3830
                     /locus_tag="VFY27_04340"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5764772.1"
                     /translation="MITASSGKLSEISPGADGIEHARNDFPALAQDINGVPIAYLDSA
                     ASSQQPECVIEKVAEYQRHDHANVHRGVHTLSHRATEAYEGARDSLVSFINAASRCEV
                     VFTSGTTGAINLVAQSWCRPRLKRGEKILITHLEHHSNIVPWQLVCEQTGAELEVCPI
                     NQRGEIDLDHFVELMDDRVRMVAVAHVSNALGTILPLEQIVAAAHERDIAVLVDGAQA
                     VPHMQVDVQALGCDFYAFSGHKMFAPTGIGVLYAKESRLDAMPPWQGGGDMILEVRFD
                     GTTYNELPYKFEAGTPNISGAVGLGAAIEYLRALGMDRVARHESALLQYMVEKLSTID
                     GLRMVGTARDKASVQSFMLGEIHPHDLGTILDHQGVAIRTGHHCAMPVMDFFGISGTA
                     RASLALYNNRQDVDRLTDALVNAGGVFA"
     gene            3827..4306
                     /locus_tag="VFY27_04345"
     CDS             3827..4306
                     /locus_tag="VFY27_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX5764773.1"
                     /translation="MSSDMDDLRDLYRELILDHAKSPRHFGKLEDATHVAQGINPLCG
                     DKLQMYFSVDEDNKISDARFEGSGCAISVASASLLTETVIGLDTERALQFFAAMVRRL
                     SGRENQKHDPDVDDIDLGKLRALEGVKEYPSRVKCATLAWHALNSALRDIRQPVSTE"
     gene            4311..4862
                     /gene="sufT"
                     /locus_tag="VFY27_04350"
     CDS             4311..4862
                     /gene="sufT"
                     /locus_tag="VFY27_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX5764774.1"
                     /translation="MFGHTNEPVSLTRDVSAVIIPAGEVVTLREGTTGFVTQALGGSF
                     TVYVEGNLFRIAGTDADALGKEPVEPPSIPENATDKDIEEVIWQQLKTCYDPEIPINI
                     VDLGLVYRCEVTKTQDGQRSVSVDMTLTAPGCGMGDILVQDAREKIAIIPTVADVRVE
                     LVFDPPWNQSMMSDEARLQTGMM"
BASE COUNT         1074 a         1511 c         1609 g          876 t
ORIGIN      
        1 gagagtttta ttcagtggcc gtggcgaaca acatgcagca ggccgatacc ggcaccaaga
       61 tgattcacat cgggcggaat tcgcgcagca cgattgtctc caagggcatc tccgcagggc
      121 gtgcacagaa tgcgtatcgc gggctggtgc gcgtcatgcc gcaggcggaa aacgcgcgca
      181 accataccca gtgcgattcg ctcctgctcg gcaaggactg tggcgcccac acgttcccct
      241 acgtggaagt gcgaaacccg acggcgaagg tcgagcacga ggccacgacg tcgaagatct
      301 ccgcggacca gcttttctac tgccgccagc gcggtatttc ggaagaggat gcggtcaaca
      361 tgatcgtgaa cggcttctgc aaggaagtgt tccgggaact gcccatggag ttcgccgtgg
      421 aagcacagaa cctgctgaac gtcagcctcg aaggcgcggt cggctaaggc cggcgagcag
      481 caaaggaaac caggacatgc ttgaaatcaa gaacctgaaa gcacgtatcg gtgaaaccgg
      541 gatattgacg ggactgagcc tgacggtgaa gcccggggag gtgcacgcca tcatggggcc
      601 gaacggctcc ggcaaaagca cactcgcact ggtgctggcc ggtcatgaag actacgtcgt
      661 tacggaaggc tccgtgacct atatgggcaa ggacctgctc ggcctggacc ctgaagaaag
      721 ggcgcggatc ggcgtgttcc tgggcttcca gtacccggtg gagattgccg gcgtcaacaa
      781 tgcctacctg ctgaaggcgg cactgaatgc caagcgccgc gcgcagggcg aaccggaggt
      841 cgatgctttt gaattcctga agctggcgcg ggaaaagatg gcgcttctcg acatggacca
      901 ggcgctgctg aaccgcggcg tcaacgaagg cttttccggc ggcgaaaaaa agcgcaacga
      961 gatactgcag atggcagtcc ttgagccgcg cctggcgatt ctcgacgaga ccgattcggg
     1021 tctcgacatt gacgcgctga aggcggtagc caaaggcgtc aactccctgc gtgacgcgga
     1081 acgagccatt gtcctggtga cgcattacca gcggctgctg gattacgtcg agccggactt
     1141 cgttcacgtc ctgtccggcg gcaagatcgt tcgttcgggc gacaagtccc tggcgcacat
     1201 gctcgaggag aaggggtacg actgggtgcg agaggcggcg aacgcatgag cacctccacc
     1261 attgatactg ccctcctcga gcgagccgtc gccaggctgc ccgcggattc gctggcacgg
     1321 gcgaggaagg aggcgctggc gcgatttgcg aagacgggat tcccgacgac gcgacacgag
     1381 gactggcgct acacgaatct cgcacccgca atcggcgtca gtaacctgtg gctgcgggag
     1441 gcggccgcgg gagccatcga ggaaggtatc ggcgaggggg tgcagcggta cgccgattgg
     1501 ctcaacacgc aaattgacgc ctggtggatc gtgatcgcga acggcgtggt gcaagaagag
     1561 tcgctcatgc gccttcgcga acgtgctccc ggcgcacttg aagcccggtt gctggcctca
     1621 gctggcgaga gcaagtgtct ggaggctgca gccatggctg ccgacgagcc aatgacccgc
     1681 ttcaatacgg cgctgctgcg cgatgccttg cgcattacag tgcacgccgg tcgcgaaatc
     1741 gagaggccca tcggcttgtt gatgatcgac agcgctggca gcacatccac cgtgagccag
     1801 tcgcgagtgt tcattgaaac cggggcgaac agccgggctg aatttatcga gtatcacgcc
     1861 tctcttggcg aacacgggca cttcgctaac gtcgtcactg aactggggat cggtcccaac
     1921 gccgccgtcg attttgtacg gatacaggat cgcgatgccg cccacttcca ggtaggcagg
     1981 ctgcacgcgg ccatgggccg cgacagcagg ctgcgtcatg cggctttcga cgtcggcggg
     2041 gccctcatac gcaatgacat cgccgtcgac atcgccgaac ccggcgctgc cgtggaactg
     2101 ctggggctct atctcgctgc ggggcggcag cacatcgata accatacgcg cgtcgaccac
     2161 aggaccggcc ccgcgaacag ctccgaagaa taccgcggca tcctcaccga tcactcccga
     2221 tgcgtgttca acgggaaggc aatcgtgcac aaaggggctg acggaacgga tgcgcagcag
     2281 gcgaatcaca acctgctgct gtcggacaag gccgagatcg acacgaagcc ggaactggaa
     2341 atttatgccg atgacgtcaa gtgcagccac ggcgcaaccg tgggccagct cgacaagacg
     2401 gccatgttct acctccgctc gcggggcttg agccgcgatg aggccgcgca ggtgctgacg
     2461 cgcgcgttcg ccgcaaggat tatcgcgaaa tcgccggtgc ccgcctcgcg tgcccacatc
     2521 gaaggtctca tcgaccgccg cctgcaggcg ctggtgggag acgtactatg attacagcca
     2581 gcagcggcaa gctcagcgag atttctccgg gagccgacgg tatcgagcac gcacgcaacg
     2641 acttccctgc cctcgcccag gacatcaacg gcgtacccat cgcttatctc gacagcgcgg
     2701 catcttcgca gcaacccgag tgcgtcatcg aaaaagtggc agagtaccag cgtcacgatc
     2761 acgcgaacgt gcaccgcggc gttcataccc tgagtcatcg tgcgacggaa gcctacgaag
     2821 gtgctcgcga ttcgctggtg tctttcatca acgcggcttc gcgatgtgag gtggtgttca
     2881 cgagcggtac aacgggcgcg atcaacctcg ttgctcaaag ctggtgccgg ccacgcctga
     2941 agcgcggcga gaaaatcctc atcacgcatc tcgagcacca ctcgaacatc gtgccgtggc
     3001 agctcgtctg cgaacagact ggggcagaac tggaggtatg cccgatcaac cagcgcggcg
     3061 agattgacct cgaccacttc gtcgaactaa tggatgaccg ggtgcggatg gttgccgtgg
     3121 cgcacgtgtc caacgcactc ggtaccatac tgccgctcga gcagatcgtg gccgcggcgc
     3181 atgagcgtga catcgcggtg ctggtagatg gtgcacaagc ggtgccccac atgcaggtcg
     3241 acgtccaggc gctcggctgc gacttctatg cgttttccgg gcacaagatg ttcgcgccga
     3301 caggcatcgg cgtgttgtac gcgaaggaat cgcggctgga tgccatgcct ccctggcagg
     3361 gcggcggcga catgattctc gaagtgcgct tcgacggcac cacctacaac gagttgccct
     3421 acaagttcga agcgggtacc ccgaacattt ccggcgcagt gggtctcggc gcggcaatcg
     3481 agtacctgcg tgctctcggc atggatcgag tagcgcgcca tgaatcggcg ctgcttcagt
     3541 acatggtgga aaagctctcc acgatagacg gccttcgcat ggtcggcact gcgcgagaca
     3601 aggcgagcgt gcaatcgttc atgctggggg agattcatcc gcacgacctc ggcacgattc
     3661 tcgaccacca gggtgtcgcg atccgcaccg gtcaccactg cgcaatgccg gtgatggatt
     3721 ttttcggcat ttccggaact gcgcgcgcat cgctggctct ctacaacaac cggcaggatg
     3781 tcgatcggct tactgacgcg cttgtcaacg cggggggggt cttcgcatga gcagtgacat
     3841 ggacgacctg cgcgatcttt accgtgagct gattctcgac cacgcgaaga gtccacggca
     3901 tttcgggaag ctcgaggacg ccactcacgt ggcgcaggga atcaatccac tgtgcggtga
     3961 caagctgcaa atgtatttct cagtggacga ggataacaag atcagcgacg cccgcttcga
     4021 ggggtccggg tgcgcaatct ccgtcgcgtc ggcttcatta ctgaccgaaa cggtcatcgg
     4081 cctcgatacc gaacgcgcgc tgcagttttt tgcagctatg gtcaggcggc tgtcgggaag
     4141 ggagaatcag aaacatgacc cggatgtcga tgacatcgat ctgggaaagc tccgggcgct
     4201 ggaaggcgtg aaggaatatc cgtcgcgggt aaaatgcgcc actctcgcgt ggcacgccct
     4261 caattcggcc ctgcgggata ttcgccagcc ggtgtctacg gagtaaagac atgtttggcc
     4321 acaccaacga acctgtgtcc ctgacgaggg acgtaagcgc ggtcatcatt cccgccggcg
     4381 aagtcgtgac actccgcgag ggcacgacgg gcttcgtcac ccaggcactg ggcggaagct
     4441 tcaccgtgta tgtggaaggt aacctcttcc gtattgcagg caccgacgcc gacgcgctcg
     4501 ggaaggagcc tgtcgagcca ccttccatac cggagaacgc gaccgacaag gacatcgagg
     4561 aagtgatctg gcagcagcta aagacatgtt acgacccgga aattccgata aacatcgtcg
     4621 acctcgggct ggtgtatcgc tgcgaggtca cgaagacaca ggacggccag cgatcggtga
     4681 gtgtcgacat gaccctgacc gctcccggct gcggcatggg tgacatcctg gtccaggacg
     4741 cgagagagaa aattgccatc atcccgaccg ttgccgacgt cagggtcgaa ctggtcttcg
     4801 acccgccctg gaaccagagc atgatgtcgg atgaagcgag actgcagact ggcatgatgt
     4861 aaactgccct caggtgcagc gcaagacgaa gccgaatgaa gaccctcacc attctccgcc
     4921 acgggaagtc cagctggagc aaagcaggcc tcccggacca ggagcggccg ctcaatccgc
     4981 gcggcgaaag agacgcccct gtgatggcgg agagaatcaa tgaggcgggc atcaggcctt
     5041 cgctaatcat gagcagcccc gctgtgcgcg
//
LOCUS       DASUOM010000210         6100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_647939,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000210 DASUOM010000000
VERSION     DASUOM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647939"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..434)
                     /gene="yccX"
                     /locus_tag="VFY27_04355"
     CDS             complement(150..434)
                     /gene="yccX"
                     /locus_tag="VFY27_04355"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF011000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5764775.1"
                     /translation="MANTTEARCRRFRVTGRVQGVFFRDSTREQARRLELTGYANNMP
                     DGTVEVVACGSEASLDSLANWLESGPPMAKVAGVETTGVPYADFRKFTIG"
     gene            complement(451..720)
                     /locus_tag="VFY27_04360"
     CDS             complement(451..720)
                     /locus_tag="VFY27_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX5764776.1"
                     /translation="MALLFLMAACGSGPPVQEMSDARQAIAVAREAGAEERAAQQLKD
                     AEALLDRAQQKLSDRDYEQARQNALQAKLKALDALARTENPPRNE"
     gene            910..1290
                     /locus_tag="VFY27_04365"
     CDS             910..1290
                     /locus_tag="VFY27_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX5764777.1"
                     /translation="MYHNPSCTTSRKTLELIREHGIEPHIVEYLKTPPDAATILKIAK
                     LLEVPVADLVRQSGPGGQAGSGDATFSSNDNPAAADWLASHPKLLQRPLIVDEEKNVA
                     VIGRPPEAVLGLLADPAAESSAGS"
     gene            1338..1913
                     /gene="wrbA"
                     /locus_tag="VFY27_04370"
     CDS             1338..1913
                     /gene="wrbA"
                     /locus_tag="VFY27_04370"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX5764778.1"
                     /translation="MYYSRKGATASLAREICQGVDAVSGASARLRTVPAVSAVCEAVE
                     PAVPGEGPPYATYEDLAECAGMILGSPTRFGNMAAPLKYWLDGTSSQWLSGTLAGKPA
                     GLFTSTSTLHGGQESTLLTMAVPLLHHGMLIVGVPYTEPVLSSTTTGGSPYGASHVTW
                     NRENDRLSEDDRTLARALGSRVARVAVKLQL"
     gene            complement(2027..2743)
                     /gene="hda"
                     /locus_tag="VFY27_04375"
     CDS             complement(2027..2743)
                     /gene="hda"
                     /locus_tag="VFY27_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX5764779.1"
                     /translation="MTQLALPLSLQDRGVFDSFWAADNAQLVAFLMRIADAASHPGTE
                     AHTGCWLWGGEATGKSHLLHAVCERLGEDAVFVPLRRFIESGPGILDGLATRRCICLD
                     DAGSIAGREDFELALFDLLNQAADRQVLLVVSARAAPRDCGFRLADLQSRLSKLPVFR
                     VEPLDEAGRVNALRLRASQRGLELPDDTARFLLSRSRRDMASLYRLLDRLDAEALVAQ
                     RRLTIPFVRSVMQRLEHATE"
     gene            complement(2745..3299)
                     /locus_tag="VFY27_04380"
     CDS             complement(2745..3299)
                     /locus_tag="VFY27_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5764780.1"
                     /translation="MSLRWLPNAISLFRIALVAPILLLILDRRYELALVLFFVAGFSD
                     GVDGYLAKRFNWHTRLGALLDPVADKLLVAGTFLMLMYTGHVPVWLAALVILRDVIIV
                     GGAAAYTFLVGPVEGEPTRISKLNTALELLFVLFVLSRAAFGWPAEISVTLIGAGVLV
                     TVVVSGVDYVWSWTRRARPHLSRH"
     gene            complement(3308..4429)
                     /locus_tag="VFY27_04385"
     CDS             complement(3308..4429)
                     /locus_tag="VFY27_04385"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX5764781.1"
                     /translation="MKSSLIKWFQVGRMRLAGISGLPVAVLALLLCTTSVGAADSSYL
                     YTARVPVEPNNPQSRERAYEAALSQVLLRITGQQNALPPEQVKEWFPNPARYVMQYRP
                     GEENTLWVTLDGATLERVLREAGYTVWGADRPLTLMWVAVDWGQGEREIVAADDPERN
                     LAEGRSIDRNRLLRERIQDVALERGLPVAFPLLDAEELELVSFSDIWGGFNDGLLEAS
                     RRYGANSILVGRVRVDTAERNRWSYFFGGDRREWTGEPEEAINMLADTLAEQFAVSSD
                     APLESISLTISGINSVEAYGEVHSFMQNLQGIEHLAIDSVSGDRIRYQVEAHGGRERL
                     QRTLGFSRVLEPDDSFDRPLVASADPDPGVLEYRYRSGD"
     gene            4613..5632
                     /gene="purM"
                     /locus_tag="VFY27_04390"
     CDS             4613..5632
                     /gene="purM"
                     /locus_tag="VFY27_04390"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5764782.1"
                     /translation="MTSKPLTYKDAGVDIEAGNALVERIKPLVARTTRPEVLTGLGGF
                     GGLFALPPGKYEEPVLVSGTDGVGTKLKLAQQLEHHDTIGIDLVAMCVNDVVVQGAEP
                     LFFLDYFACGRLDIQVAADVIGGIARGCEQAGAALIGGETAEMPDMYPDGEYDLAGFC
                     VGAVERRELIDGHSIEENDVVIGIASSGPHSNGYSLIRRVLERSGTADIDGRPAAKCL
                     LAPTRIYVKPILALMQQVGIRGLAHITGGGITENLPRILPPGLHAEIDTQSWQQGPVF
                     DWLAAAGCIAAAEMRRTFNCGVGMIVVVAPDDADTALASLGSSGEKAWLLGRIAAGSE
                     GVRYI"
     gene            5641..>6100
                     /gene="purN"
                     /locus_tag="VFY27_04395"
     CDS             5641..>6100
                     /gene="purN"
                     /locus_tag="VFY27_04395"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5764783.1"
                     /translation="MSAAVLVSGGGTNLQAFIDEVREHRLDLELRVVISNNRDAGALD
                     RARNAGIADECVCNTDYAGRADFDAALAATIDRFHPDLLILAGFMRILTKAFVNRYQG
                     RILNIHPSLLPRFPGLDTHQRALDEGERVHGSTVHFVTEDLDAGPPIMQGR"
BASE COUNT         1192 a         1972 c         1857 g         1079 t
ORIGIN      
        1 ctcaagtatt acctggacat ggaaaaaggc gacatgcagc gcgccctcgg ccggtacaac
       61 ggaagtctcg gcaagcggga ctacccgaac ctcgtcctgg accgcctgcg gaagaagtgg
      121 tttcgaatct gaagcgttac aggagcggct tagccgatcg tgaatttcct gaaatcggcg
      181 taaggcacgc ctgttgtttc gacgcctgct acctttgcca tcggcggtcc cgactcgagc
      241 caatttgcca gcgagtcgag tgaggcttcg ctgccgcaag caacgacctc cacggtaccg
      301 tcgggcatgt tgttcgcgta accagtgagc tcgagccttc gcgcttgctc gcgggtgctg
      361 tcacgaaaaa acacaccctg tacgcgcccg gtcacgcgaa acctgcggca ccttgcttcg
      421 gtcgtattgg ccaccccggc agctataggc tcactcgttc cgcggcgggt tttccgtcct
      481 ggcgagtgcg tccagtgcct tgagcttcgc ctgcagggca ttctggcggg cctgctcgta
      541 gtcgcgatcg gaaagctttt gctgggcgcg gtccagcaat gcttcggcat ccttcagctg
      601 ttgcgccgcg cgttcctcgg cgccggcttc ccgggcaacc gcgatcgcct gcctcgcatc
      661 gctcatttcc tgtaccgggg gaccgcttcc gcaggcagcc atgagaaaca agagtgccat
      721 cgggagaccg ccgcgcctga ctgaactagt catgcggcgg ggctttacag acagggtggg
      781 cgtacgcacg gcggaaagct agcaagggcg taccatggca gtcaagttga gtcaaaaggg
      841 cctcgaaggt tatatcctag gagcgctata cacagcttcc atgggacgtg ccgaatcctg
      901 accatcacaa tctaccacaa cccgtcttgt accacgtcga ggaaaacgct cgagttgatt
      961 cgggagcacg gcatcgagcc ccacatcgtc gaatacctga aaacacctcc ggacgcggca
     1021 acgatcctga agatcgccaa gctgctcgag gttcctgttg cggatctcgt ccgccagtcg
     1081 gggccgggcg gccaggccgg cagcggcgat gcgacctttt cgtcgaacga caatcccgcc
     1141 gccgccgact ggctggccag ccatccgaag cttttgcagc gacccctgat cgtggacgag
     1201 gagaagaacg tggccgttat cgggcgcccg ccggaggccg tgctcgggct gctggcggac
     1261 ccggcagcgg aatcctccgc aggatcctag gcggggccag cgataacttt cggagatcgg
     1321 caatagacat actggttgtg tattactccc gcaagggagc gaccgcgtca ctcgcgcggg
     1381 aaatctgcca gggcgtcgac gccgtgtccg gtgcgtcggc aaggctgcgg acagtgcctg
     1441 ccgtttccgc cgtttgcgaa gcggtggaac ctgcggtgcc gggagagggg cctccgtacg
     1501 cgacctacga agacctggcg gaatgtgccg gcatgatcct cggcagcccg actcgcttcg
     1561 gcaacatggc cgcacccctg aagtactggc tcgacggtac cagcagccag tggctcagcg
     1621 gcactcttgc aggcaagccg gcggggcttt tcacgtcgac gtccacgctg catggcggac
     1681 aggaaagtac gctgttgaca atggccgttc cgctcctgca tcacggtatg ctgattgtcg
     1741 gcgttcctta tacggaacct gtcctctcat ccacaacgac cggcggctct ccctacggcg
     1801 ccagccacgt tacctggaac cgtgaaaatg accggctgtc ggaagacgac aggacgctcg
     1861 cgcgggcgct cggctcacga gttgccaggg tggcagtgaa gctgcagctg taagagccgc
     1921 ctcacggacg acaatcggca gcgcgtatcc acaccaaatg tcgcccgcgg ggcgggctcc
     1981 tgcagcgtgc gactcccacc cctcgcagct ggcgggtcgc tcgaccctac tccgtcgcat
     2041 gctccagccg ctgcatcacg ctccggacga acggaatcgt caggcggcgt tgtgcgacga
     2101 gtgcttccgc gtcgagcctg tcgagaagcc ggtacaggct tgccatatcg cgcctgcttc
     2161 ggctaagcag gaatcgcgcc gtatcgtcag gtagttccag tccccgctgg ctggcacgca
     2221 agcgtagtgc attgacacga cccgcctcgt ccagcggttc cacccggaaa acaggcagct
     2281 tcgacaggcg gctttgcagg tctgcgagcc ggaagccaca atcacgcggt gccgcgcggg
     2341 ccgaaaccac cagcaatacc tgccgatcgg ccgcctggtt gagcaaatcg aacagcgcca
     2401 gctcgaaatc ttcccgacct gctatcgaac cggcgtcgtc gagacagatg cagcggcgag
     2461 tagcaaggcc gtcgaggatg cccggaccgg actcgatgaa acggcggagc ggcacgaaga
     2521 cggcatcctc accgagccgt tcgcagaccg cgtgcagcag atgcgatttg ccggtcgcct
     2581 cgccacccca caaccagcag ccggtgtgcg cttcagtccc tggatggctg gcggcatccg
     2641 cgatccgcat gagaaaagcc accagttggg cattgtcggc ggcccagaag ctgtcgaaga
     2701 cacccctgtc ctgaagcgac agcggcaagg cgagctgagt caactcaatg cctcgacagg
     2761 tgtggccgcg cgcggcgcgt ccaggaccag acgtagtcga cgccgctcac gaccaccgtc
     2821 acgagtaccc cggctccgat cagggtaacg ctgatttcgg caggccagcc gaacgccgcg
     2881 cggctcagga cgaacagtac gaacaggagt tccagtgcgg tgttcagctt gctgatccgc
     2941 gtcggctcgc cctctacggg acctacgagg aaagtgtagg ccgccgcgcc gccgacgatg
     3001 atcacgtcgc gaagaatcac cagcgccgca agccagacag gcacgtgccc cgtgtacatc
     3061 agcatcagga aggtgcccgc gaccagtaac ttgtcggcaa cagggtccag cagcgctccg
     3121 agccgcgtat gccaattgaa gcgctttgca agatagccgt ccacgccgtc ggaaaagccg
     3181 gccacgaaaa agagtaccag cgccagctca tagcgtcggt cgagaatgag cagcaggatc
     3241 ggcgcgacca gcgcaatgcg gaacagcgat attgcgtttg gcagccagcg caaagacatg
     3301 cggaaggtca gtctccggat cggtaacggt attcaagcac acccgggtcc ggatccgcag
     3361 acgctacgag cggccggtcg aacgaatcgt ccggttccag caccctgctg aatccaaggg
     3421 tgcgctgcag ccgttccctc ccgccgtgtg cctcgacctg gtaacggatg cggtcgccac
     3481 tcaccgaatc tatcgcaaga tgttcgatcc cctgcaagtt ctgcatgaag ctgtgaactt
     3541 caccgtaagc ttcaaccgag ttgatgcccg aaatggtcag gctgatcgac tcgagcggtg
     3601 cgtcgctgga tacggcaaac tgttcggcca gcgtgtcggc aagcatattg atcgcttctt
     3661 ccggttcacc ggtccactcg cgccggtcgc cgccgaagaa ataggaccag cggttacgct
     3721 ctgccgtatc gacgcgcacc cgcccaacca ggattgaatt cgcaccatag cggcgcgaag
     3781 cttccagcaa gccgtcgttg aagccgcccc agatgtcgct gaagctgacc agttccagtt
     3841 cttccgcgtc gagcagcggg aacgcgacgg ggaggcctcg ttccagcgca acatcctgga
     3901 tcctttcccg gagcaacctg ttccggtcga tcgaacgccc ttccgccagg tttcgttcgg
     3961 ggtcgtcggc ggcgacaatc tcacgttcgc cctggcccca gtcgaccgcg acccacatca
     4021 gcgtcaacgg ccggtctgcc ccccagacgg tataacctgc ctcgcgcaac acacgctcaa
     4081 gtgtcgcgcc atcgagcgtg acccaaagcg tgttttcctc gcccgggcga tactgcatga
     4141 catagcgagc ggggttcgga aaccattcct tgacctgctc cggaggaagc gcattctgtt
     4201 gccccgtgat ccgcagcaaa acctgtgaga gtgcggcttc gtaggcacgc tcgcgcgact
     4261 gcgggttatt cggttccacc ggtacccgcg cggtataaag gtaggaagaa tcggcggcac
     4321 caacgctggt tgtacacaac agaagcgcca gcacagcaac gggcaggccg gaaataccgg
     4381 caagccgcat ccggcccacc tggaaccatt taatcaaact actcttcatc cactatcgcc
     4441 tgcttgatgc gctcgaacgc gggaactata cacgcgggta ccggacttgc acgcccgtct
     4501 ccgacgtgtc caatctgttc gcaaaccact attatattgt ctcacgccag tgtcctcagt
     4561 catccctgac tcaggacacc aagagcagta ccctccctga ccaggcagga acatgaccag
     4621 caaacctctt acatacaagg atgccggagt cgacatagag gccggcaacg cactggtcga
     4681 gcgcataaag ccgcttgtgg ctcgcaccac ccggccggag gtactgaccg ggctcggcgg
     4741 cttcggcggg ctgttcgcgt tgcccccggg caaatatgaa gagccggttc tggtatccgg
     4801 taccgacggt gtcggcacga agctgaaact ggcccagcag ctcgagcatc acgacaccat
     4861 aggcatcgat ctcgtcgcga tgtgcgttaa cgacgttgtc gtgcagggcg ctgagccatt
     4921 gtttttcctg gactatttcg cttgtgggcg gctcgatatc caggttgcgg cggacgttat
     4981 cggcggcatc gcccgtggtt gtgaacaggc cggcgcggcc ttgattggcg gcgagaccgc
     5041 cgagatgccg gacatgtatc cggacggcga atacgacctc gcgggttttt gtgtcggcgc
     5101 agtggagcgt cgtgaactga tcgacggcca ctcgatcgag gagaacgacg tagtcatcgg
     5161 catcgcttcg agcggccccc attccaatgg ctactccctg atccggcgcg tgctcgagcg
     5221 atcaggcact gccgacatcg atggacggcc ggctgcgaag tgtctgcttg caccgacaag
     5281 gatctacgtc aagccgatac tcgcgctgat gcaacaggtc ggcatcaggg gtctcgcgca
     5341 cataacaggc ggtggcatta ccgaaaacct gccgcgcatc ctgccgccgg gcctgcacgc
     5401 ggaaatcgat acgcagagct ggcagcaggg tcccgtcttc gattggcttg ccgctgccgg
     5461 atgcatcgcc gcggccgaga tgcgtcggac gttcaactgt ggcgtcggca tgatcgtcgt
     5521 tgtcgcgccg gacgatgcgg acacggcgct cgcttccctc gggtcatccg gcgagaaagc
     5581 gtggctgctc gggcgcatcg ccgccggcag cgaaggggtg cgttacattt gaccggccgc
     5641 atgtccgcag ccgtcctggt atccggcggc ggcaccaacc tgcaggcgtt catcgatgag
     5701 gtgcgcgagc accgcctcga cctcgagttg cgcgtcgtca tcagcaacaa ccgcgacgcc
     5761 ggggctctcg acagggcgcg caatgcaggc atcgcagatg agtgcgtatg caataccgac
     5821 tacgccgggc gcgccgactt cgacgcggca cttgccgcca ccatcgaccg cttccaccca
     5881 gacctgctga ttctcgccgg cttcatgcgc atcctgacaa aggcgttcgt caatcggtac
     5941 caggggcgca ttcttaatat acatccctca cttctgccgc gtttcccggg gctcgacacg
     6001 caccagcggg cactggacga gggtgaacgc gtgcacggca gcacggtgca tttcgtcacg
     6061 gaggatctcg acgcaggccc gccgatcatg caaggccgcg
//
LOCUS       DASUOM010000211         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_648850,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000211 DASUOM010000000
VERSION     DASUOM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648850"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFY27_04400"
     CDS             <1..315
                     /locus_tag="VFY27_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX5764784.1"
                     /translation="IGSLLAHVRRGDVVKVHSLRRGSAEAIEAIAHGSEESSKVVGRR
                     VEEIGLPRGTSIIAIVRGDEVIIAHHDVVIESDDHVILFMTDRRRIEQLEELFQVDVA
                     FV"
     gene            355..1806
                     /locus_tag="VFY27_04405"
     CDS             355..1806
                     /locus_tag="VFY27_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX5764785.1"
                     /translation="MNWTVAQRILGLLLMLFSLTMLTPLIFSLIYRGEAWLSFIQAFL
                     VTLSFGFVLWLPVRNIRKELRLRDGFVVVASFWIVLGLFGALPFYLSELPAMTLTDAV
                     FESMSGLTTTGATVLTGLDGLPKSILFYRQQLQWLGGMGIIVLAVAILPMLGVGGMQL
                     YRAEMPGPVKDTKLTPRVAETAKALWYVYLAFTALCVIGYKITGMDWFDSLCHAFSTV
                     AIGGFSTHDLSFAHFNSAAVDAVAVVFMFMAGINFSLHFFGWRYLSVSHYFENPEFRA
                     YVFVLLTVSIVVIASLLWTGTYGDPRANVINGLFQAVSIATTTGFTTADFSSWPGALP
                     VLLIFSSFIGGCAASAAGGIKVIRWLLIYKQGVREIVRLVHPNAELPVKLGRQAVPVR
                     VVDAVWGFFSVYMIVFGLMMLAMLTTGLDQVTAFSAVAASLNNLGPGLGEVAGGFMTV
                     NDSAKWIAMIGMLLGRLEIFTLLVLVTPTFWRR"
     gene            1803..2132
                     /locus_tag="VFY27_04410"
     CDS             1803..2132
                     /locus_tag="VFY27_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5764786.1"
                     /translation="MSSERISALLTMLERGQDSALLRFSLGNEYLGIKSYDEAIGHLR
                     RAIELDRKYSAAWKLLGRSLAEEGREDEAILVYRDGIEVAEQHGDRQAAKEMNVFLKR
                     LKKKQNG"
     gene            2152..2691
                     /locus_tag="VFY27_04415"
     CDS             2152..2691
                     /locus_tag="VFY27_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX5764787.1"
                     /translation="MTTSDRLVTAIDALNRAIGAGVAWLTLGMVLFTFAIVVLRYAFD
                     IGPVWMQESVTWMHAAVFMLGGAYALQRDEHVRVDIFYRGMSPVGRARIDIAGLLLFL
                     FPLCAFVIYESFDYVAASWAIREGARDAGGLPYPLISLLKSILLVMPLLVALQGLSLL
                     LTSVRNLRERRLHAAKLEP"
     gene            2721..>3149
                     /locus_tag="VFY27_04420"
     CDS             2721..>3149
                     /locus_tag="VFY27_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5764788.1"
                     /translation="MEWVALLLFAAVILLLMGGFPVAFTLAGTGLAFAFAGVIGGGFE
                     SAFLTGMPSRLFGIMSNDTLVAVPLFVFMGITLERARIAEDLLDTLSSLFGRLRGGLG
                     ISVTLVGMLLAASTGIVGATVVTMGLLSLPTMLKRGYSPEV"
BASE COUNT          518 a          915 c         1002 g          714 t
ORIGIN      
        1 atcggctcgc tgctcgccca cgtcaggcgt ggcgacgtcg tcaaggtgca ttcgctccgg
       61 aggggctccg ctgaggccat cgaggccata gcacacggat cagaggagag ttccaaagtg
      121 gtcgggcgac gggtggagga aatcgggttg ccgcgcggca cgagcatcat cgccatcgtg
      181 cggggagatg aggtcatcat cgctcaccac gatgtcgtga tcgaatccga cgatcacgtc
      241 atcctcttca tgacggaccg gcgcaggatc gagcaactgg aagagctgtt ccaggtggat
      301 gtggctttcg tctgaggacg cgggcgcatc gagccacgcg cagggcgcta atgcatgaac
      361 tggacagttg cgcagaggat tctcgggctg ttgctgatgt tgttcagcct gacgatgctc
      421 acgccgctca tcttcagcct gatctaccgc ggcgaggcat ggttgtcctt tatccaggct
      481 ttcctggtaa ctctgtcgtt cggcttcgtc ttgtggctgc ccgttcgcaa catccgcaag
      541 gagctgcgcc tgcgcgacgg cttcgtggta gtggcatcct tctggatcgt actcggcctg
      601 ttcggtgccc tgccgttcta tctgagcgaa cttcccgcga tgaccctgac cgatgcggtg
      661 ttcgaatcca tgtcgggact tacgaccacc ggggcgaccg tcctcaccgg gctcgacggc
      721 ttgcccaagt ccatcctgtt ttaccggcag caactgcagt ggctgggcgg catgggcatc
      781 atcgtattgg ccgtcgccat attgccgatg ctcggcgtcg gcggaatgca gctgtatcga
      841 gccgagatgc cgggtcccgt caaggacacc aagctgacgc ccagggtggc cgagaccgcc
      901 aaggcgcttt ggtatgtata ccttgctttt acagctcttt gcgtgatcgg ctacaagata
      961 accggcatgg actggttcga ctcgctgtgt catgcatttt cgacggtagc cattggcggc
     1021 tttagtaccc atgatctgag ctttgcgcat ttcaatagcg ctgccgtcga cgctgtcgca
     1081 gtcgttttca tgttcatggc ggggatcaat ttttcgctgc acttcttcgg ctggcgatat
     1141 ctgagcgtca gccattactt cgagaacccg gagtttcgcg cgtacgtttt cgtcctcctg
     1201 accgtatcga tcgtcgtgat cgcgtcactg ctgtggaccg gcacatacgg cgatccccgc
     1261 gccaatgtca tcaatggttt atttcaggcg gtatccatag cgacgacgac cggcttcact
     1321 actgcggact tctcgtcctg gccgggagcg ctgccggtcc tactgatctt ttcgagcttc
     1381 atcggcggct gcgccgcctc ggcggccggc ggaatcaagg tcattcggtg gttgctgatc
     1441 tacaaacagg gtgtacgcga gatcgtgcgg ctggttcacc ccaacgcgga gcttccggtg
     1501 aagctcggca ggcaggcagt gccggtacgc gtggtggatg ccgtgtgggg cttcttttcc
     1561 gtgtacatga tcgtgttcgg cctgatgatg ctggcaatgc tgacgacagg tctcgaccag
     1621 gtaacggcgt tttccgccgt cgccgccagc ctgaataacc tcgggccggg cctcggcgag
     1681 gtcgcgggag gcttcatgac cgtgaatgac agcgcgaaat ggattgccat gatcggcatg
     1741 cttctcgggc ggctggaaat ctttacgctg ctcgtactgg ttacgccgac gttctggcgg
     1801 cgatgagctc cgagcggata agcgcgctgc tcacgatgct ggagcgcggc caggattccg
     1861 ccttgttgcg cttttcgctc ggcaacgagt accttggcat caagtcctac gatgaagcca
     1921 tcggccatct gaggcgcgcg atagagctgg atcggaaata ctcagccgcc tggaaactcc
     1981 tggggcgttc gctggcggaa gagggccggg aagacgaagc gatcctcgtg tatcgcgacg
     2041 gcatcgaagt ggcggagcag cacggcgacc gccaggcggc gaaagagatg aacgtgtttc
     2101 tcaagcggct gaagaaaaag caaaacggct gatgtacttt cagggagaga tgtgactaca
     2161 agcgaccgcc tggttacggc tattgatgcg ctgaaccggg ccataggcgc cggggtggca
     2221 tggctcaccc tggggatggt gctgttcact ttcgcgatcg tcgtgttgcg ctacgccttc
     2281 gacatcggac ccgtctggat gcaggagtcg gttacctgga tgcacgccgc ggtgttcatg
     2341 ctcggcggcg cttatgcgct gcagcgggac gagcatgtgc gcgtcgacat cttttaccgc
     2401 ggcatgagcc cggtagggcg cgcacggatc gatatcgcag ggttgttgct cttcctgttc
     2461 ccgctgtgcg cctttgtcat ctacgagtcc ttcgactacg tggcggcctc ctgggcgatt
     2521 cgcgaaggcg cacgcgatgc aggcggtctg ccatacccct tgatctcgct gctgaagtcg
     2581 atcctgctgg ttatgcccct gctggtggcg ctacagggcc tgtcgctgct gctgacctcg
     2641 gtgcgcaatt tgcgcgagcg ccgcttgcac gcagcaaagc tcgagccgtg aacaaaccgc
     2701 aactgcggag gacgggctga atggagtggg tcgcccttct tctgttcgct gccgtgatcc
     2761 ttttgctcat ggggggcttt ccggtcgcgt tcacgctggc cggcaccgga ctggcctttg
     2821 ccttcgccgg cgttatcggt ggcggcttcg aatctgcttt cctgacagga atgccgagcc
     2881 gcctgttcgg catcatgtca aacgacaccc tcgtcgccgt tcccctgttc gttttcatgg
     2941 ggattaccct cgagcgggcg cgcattgcag aagacctgct agacaccttg agttcccttt
     3001 tcggcaggct gcgcggcggg ctgggcattt ccgtgacgct ggtcggcatg ctgctcgccg
     3061 cgagcaccgg aatcgtcggc gcgaccgtcg tgactatggg gcttctttcc ctgccgacga
     3121 tgctgaagcg ggggtattcg ccggaggtc
//
LOCUS       DASUOM010000212         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1698404,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000212 DASUOM010000000
VERSION     DASUOM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698404"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFY27_04425"
     CDS             complement(<1..757)
                     /locus_tag="VFY27_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5764789.1"
                     /translation="MGAFEFVALDKAGKESRGLLEGDTAKHVRQILRERQLLPVKVTE
                     VTQRDSSKQKSFSIRRGLSASELALLTRQLATLSQSGLPLEEALLAVSQQNENPRAQS
                     ILLGVRSKVMEGHTLADGLGDFPAAFPELYRATVAAGEQSGHLDAVLERLAEFTESRQ
                     ILQQQVRNALIYPVALVVTAAAIISFMLAYVVPKVVTIFENYNQQLPILTRMMLWTSD
                     FLRDHWILIIIGIAATIYGARRLLRNEGPRRHYH"
     gene            complement(765..2300)
                     /gene="gspE"
                     /locus_tag="VFY27_04430"
     CDS             complement(765..2300)
                     /gene="gspE"
                     /locus_tag="VFY27_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX5764790.1"
                     /translation="MASHTELFLETEETTAPDVVRIAEPEARPLPFSFAKRHGVLIRQ
                     IRDGAADTVYRTGTLPLSLAEARRFAGVPLRLTRVTPAAFDAMLQQTYEQGSHKAMQM
                     VGDLDEQTDLLQVAQELPEPSDLLESDDDAPIIRFINAVLTEAVKDNASDVHIEPYEN
                     RLNVRFRIDGVLQEILQTRRALAPLVVSRIKVMSKLDIAEKRLPQDGRISLRIAGRAV
                     DVRVSTMPSGHGERVVLRLLDKQAGRLDLSSLGMDGDSLAVIDDLIHKPHGIILVTGP
                     TGSGKTTTLYAALERINDNSRNIMTVEDPIEYFIDGIGQSQVNTKVEMTFARGLRAIL
                     RQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTASGAVTRLRDMGVEPFLLS
                     SSLIGVLAQRLVRVLDDDTKVAYTARDYECRTLNLDPADPPTLYRPGTGDGTGYRGRT
                     GIYELIAVDDRMRTMIHDGVSEQEFERYARTLSPSIRDDGRRRVLAGDTTLEEVLRVT
                     RAD"
BASE COUNT          423 a          728 c          802 g          495 t
ORIGIN      
        1 tgtgataatg ccgacgcgga ccttcattgc gcagcagccg cctcgcgccg tatatcgtcg
       61 cggcaatgcc gatgatgatg aggatccagt ggtcccgcag gaagtcgctg gtccacaaca
      121 tcatgcgggt caggatcggc agctgctggt tgtagttctc gaaaatggtg acaaccttcg
      181 gcacgacata ggccagcatg aagctgatga ttgccgccgc cgtgaccacg agggcaacgg
      241 ggtaaatcag tgcattgcgt acctgttgct gcaggatctg gcgcgattcg gtgaactccg
      301 ccagccgctc gagtacggca tcgaggtgtc ccgactgttc gccggcggct acggtggcgc
      361 gatagagttc cgggaacgct gccggaaaat ctcccaggcc gtcggcgagc gtatggcctt
      421 ccatgacttt cgagcgcacg ccaaggagaa tgctttgcgc acgcggattc tcattctgct
      481 ggctgactgc cagcagcgct tcttcgaggg gcagcccgga ttgcgatagc gtcgcgagct
      541 gtcgcgtcag tagcgccagt tcgctggcgg ataggccgcg ccggatggag aagctctttt
      601 gcttgctgga atcccgctgt gtgacctcgg tgaccttaac cggcagaagc tggcgctcgc
      661 ggagaatctg gcgaacgtgc ttggccgtgt cgccttccag cagaccgcgc gattccttgc
      721 cggccttgtc gagtgcaacg aactcaaacg cgcccatagt ggggctagtc tgcgcgggtc
      781 actcgaagca cttcttccag cgtcgtgtcg cccgcgagta cgcggcgacg gccgtcgtcg
      841 cggatgctgg ggctcaacgt ccgggcataa cgctcgaact cctgctcgct gacgccgtcg
      901 tgaatcatgg tgcgcatgcg gtcgtctacc gcgatgagtt cgtagatgcc ggtgcgcccg
      961 cgatagccgg tgccgtcgcc cgttccgggg cggtacaggg taggagggtc ggccgggtcc
     1021 aggttcagtg tgcggcactc gtagtcacga gccgtgtagg caaccttggt atcgtcgtcg
     1081 agcacgcgca cgaggcgctg tgcgagaaca ccaatgaggc tggaggagag caggaacggt
     1141 tccacaccca tgtcgcggag gcgcgttacc gccccggagg ctgtgttggt atgcagggtg
     1201 gaaagcacca ggtggcccgt caggctcgcc tgaacagcga tctccgcagt ttccaggtcg
     1261 cggatttcgc caaccatcac gacgtccggg tcctggcgca ggatggcgcg caatccgcga
     1321 gcgaaggtca tctcgacctt cgtgttgacc tgcgactggc cgatgccgtc gatgaagtat
     1381 tcgatcggat cctcgacggt catgatgttg cggctgttgt cgttgatgcg ctcgagcgcc
     1441 gcatacaggg tggttgtctt gcccgaaccc gtgggcccgg tgacgaggat gatgccgtgc
     1501 ggtttgtgga tcaggtcgtc gatgacggcg agcgaatcgc cgtccatgcc gagcgaagag
     1561 aggtcgagtc ggcccgcctg cttgtcgagc agtcgcagca cgacccgctc gccatggccg
     1621 gacggcatgg tcgatacgcg cacgtctacg gcgcgacctg cgatccgcaa cgagatgcgc
     1681 ccgtcctgcg gtaagcgctt ctccgcaatg tcgagcttcg acatgacctt gatgcgcgac
     1741 acgaccaggg gggccagcgc acgccgtgtc tggaggatct cctgcaggac accgtcaatg
     1801 cgaaagcgca cgttcagacg gttttcgtac ggctcgatat ggacgtcaga cgcgttgtcc
     1861 ttgaccgctt cggtcagcac ggcgttgatg aaacggatga ttggggcgtc gtcgtcgctt
     1921 tccaggaggt ctgacggttc cggaagctcc tgcgcgacct gcaggagatc cgtctgctcg
     1981 tcgagatcgc ccaccatctg catggctttg tggctgcctt gctcgtaggt ctgctgcagc
     2041 atggcgtcga aggctgccgg tgtgactctt gtcaggcgca gtggtacgcc agcgaaacgc
     2101 cgcgcttcag cgaggctcag cggcaaggtt cccgtgcgat aaaccgtatc ggccgctccg
     2161 tccctgatct gccggatcag gacgccatgg cgcttggcga aagaaaaggg cagcggcctt
     2221 gcctccggtt cggcgatgcg cacgacatcg ggtgcggtcg tttcttcggt ttcaagaaat
     2281 aattccgtat gacttgccat ccgggttact cggactcgtc cggaactttc gacaactctt
     2341 cctcaatcac ttcgtcggga ttgggtatga caggcttcgc ccgcccgtat tcctcgatcg
     2401 gcggaagcat gggacgttcc gtccgcggca tcaacgcgac atcgccga
//
LOCUS       DASUOM010000213         5002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1646448,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000213 DASUOM010000000
VERSION     DASUOM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646448"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFY27_04435"
     CDS             <1..1020
                     /locus_tag="VFY27_04435"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5764791.1"
                     /translation="IGPVAALVALLAAGFTPIRSVSFAIFALIGVSWLSKTPMTATRC
                     FDAVVDAMRTMAATAVLLVAVGLVINVVTTTGLGNAFSLMIVEWANGSLIATLGLIAI
                     ASLVLGMGLPVTASYVVLATLAAPMLFDLVSRDAMLSVMQSAELPSSVRATLELFGGD
                     PVVALREMPLEMKQLMRQELLAPELLSGMLLSAHLVIFWLSQDSNVTPPVCLVAFAAA
                     GIAGSRPMATGFTAWKLAKGLYLVPLLFVYGSLISGSWAERLEAFAWACLGLYALCGL
                     LQWHLGRPLNIVTALLLTLSAVLLLWVPFGSWMHFVGAILLAGIVAWQRRAAMPVSGG
                     EHAAP"
     gene            1073..3181
                     /locus_tag="VFY27_04440"
     CDS             1073..3181
                     /locus_tag="VFY27_04440"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5764792.1"
                     /translation="MLYGAAILTGAALVFQVQPIAGKLLLPSFGGGASVWTTCMFFFQ
                     TMLLAGYGYAHALTRLQNPRRQAALHALLLVISLLFLPVGVSADPLDSGLRDPMALIL
                     LALLFSTGVPFIMLASTAPLLQRWSAMTNPRRSPWRLYALSNFGALLALLSYPLVVEP
                     GLSLRAQTAVWTGGYLLFAAIMFGACRLVWKWHGASPACEQADAGRREQARPSSPVSF
                     LTILLAASGVIILLAVTNEITQNIAPVPFLWILPLVTYLLTYIICFSGERWYDRAIWG
                     SLFVVSASSLVILHFFGTSFPIVPVIVAYLLVQFCASIVCHAELYRLRPEPSRLTGWY
                     LQIALGGAIGGAFVSVVAPLAFSRYWEALVGVYLVYVVLGIVVLRESREPPEKKPFHV
                     VSAIQRKLERWALRLFALGWVTGLFLLPAVVLSLDKLRVRYDVTSSRNFYGLLNVRDV
                     VTDGEARRLLVDGTTIHGFQLLDEKRQDVPTSYYGANTGIAMVMENVERPVTGLNVGI
                     VGLGTGTLAVYGKPNDRFRFYELNPAVVDVANTHFSFLRQSPADVDIVTGDGRVAMER
                     ELELEGSREYDLLVIDAFNSDAIPVHLLTLEALELYWSHLKPDGVLALHLTNNYLDLV
                     PVASNLGASLGKEVLFVTTPRDVGVSSAADWVLLAEDPALFAPGTGEHAGSELTVPGQ
                     ERLWTDDYSNLLGTLKRGRK"
     gene            complement(3237..3815)
                     /locus_tag="VFY27_04445"
     CDS             complement(3237..3815)
                     /locus_tag="VFY27_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX5764793.1"
                     /translation="MARTIARDTTRATTRTPVQQAAFITGVVFLIVGILGFIPGITTN
                     YSAMEFAGHESQAMLLGIFQVSVLHNIVHLAFGVAGITMSKTSPGARRFLMVGGILYL
                     LLWLYGLVVNEASEANFVPLNNADDWLHLFLGAGMIALSFLSRDRVPVQRDAATTHRT
                     TTTTTRDTTRDPVDRTSARTTRDPVDRDPDLR"
     gene            4148..4633
                     /locus_tag="VFY27_04450"
     CDS             4148..4633
                     /locus_tag="VFY27_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX5764794.1"
                     /translation="MLKIIRLPFPWALFVMAGTVSACAYMPNNDPLAISVAGVESLPG
                     EGMEMRLAVKVRIQNPNDLPVDYTGAALTLDLNGRKLASGVMGESGTVPRYGEVVMVI
                     PVTISAFDMARQVFGVANASGQSDVHYRVRGKLEAGLFGTRRFSDEGMFQLDQPVEPG
                     S"
     gene            4617..>5002
                     /locus_tag="VFY27_04455"
     CDS             4617..>5002
                     /locus_tag="VFY27_04455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5764795.1"
                     /translation="MNQVHEGCRLRSTILVPRRLWPVRGTGDSLLSQQDRIKWDEKYR
                     AGAYAGRDHPTRLLAEWVPQANRGRALDVACGAGRNSVFLAVQGYLVDAVDISAAGLE
                     CARRSAETRGVHVHWIEADLETGAASA"
BASE COUNT          864 a         1484 c         1585 g         1069 t
ORIGIN      
        1 atcggaccag tggccgcatt ggtcgccttg cttgctgcag gtttcacgcc gattcgctct
       61 gtctcgttcg cgatattcgc cttgatcggg gtttcctggt tgtcgaagac gccaatgacc
      121 gcaacgcgct gtttcgacgc agttgtcgat gcgatgcgaa ccatggcagc tacggcggtc
      181 ttgctggtag cggtgggact ggtgataaac gtcgtcacca cgaccggcct cggcaatgcg
      241 ttctcgctga tgatcgtcga atgggcgaac ggcagcctga ttgcgacatt ggggctgatc
      301 gctatagcgt cgctggtatt gggcatgggc ctgcctgtta ccgcttccta cgttgtactt
      361 gcgacgctcg cagcgcccat gctgttcgac ctcgtcagtc gcgatgcgat gctttccgtg
      421 atgcagtctg cggaactgcc ctcgagcgtc cgtgcaacgc tcgagttgtt cggcggcgac
      481 cccgtggtcg cgctgcggga aatgccgctc gagatgaaac agctcatgcg gcaggagttg
      541 ttggcgcccg aactactatc gggaatgctc ctgagcgcac atctcgtcat cttctggctc
      601 tcccaggaca gcaacgtcac cccgcccgtg tgcctggtgg cattcgcagc ggcgggaatc
      661 gccggtagcc ggccaatggc aaccggcttc accgcctgga aattggcgaa agggctttac
      721 ctggtgccgt tactgttcgt ctacgggtcc ctgatcagcg gctcctgggc agaacggctg
      781 gaagcgttcg cctgggcgtg cctcggtctc tacgcattgt gcgggctgct gcagtggcat
      841 ctcggacgac cgttgaacat cgtcaccgcg ctactactca cgctgagcgc cgtgctgttg
      901 ctgtgggtcc cctttggctc ctggatgcac tttgtgggtg caattctgct ggccggcatc
      961 gttgcatggc aaaggcgggc tgcgatgccg gtgagcggcg gagagcatgc ggccccttga
     1021 gcgaggcttt tcagtagcgc gtgggtctgc ccggcgttcc cgggctgatg cgttgctgta
     1081 cggcgctgcg atactcaccg gggccgcgct ggtattccag gtccagccaa tcgccggaaa
     1141 gttgctgttg ccatcgttcg gcggcggcgc ctccgtgtgg accacatgca tgttcttttt
     1201 ccagaccatg cttctcgcgg gctacggcta cgcccatgca ctgaccaggt tgcagaaccc
     1261 gcgccggcaa gctgcgctac atgccctcct gctcgttatc tccctgctat tcctgccggt
     1321 cggcgtttca gccgacccgc tcgattcggg gctgcgggat ccgatggcgt tgatcctgct
     1381 ggcattgctg ttttcgaccg gcgtcccgtt catcatgctg gcaagcactg ctccgttgct
     1441 ccagcgatgg tcggcaatga cgaacccgcg gcgctcgccc tggcgcctgt acgcgttgtc
     1501 gaacttcggc gcattgctgg cgctgctcag ctatccgctg gtcgtggagc ccggattgag
     1561 cctgcgggcg cagacggccg tgtggacggg aggctacctc ttgttcgcgg cgatcatgtt
     1621 tggcgcttgc cgcctggtgt ggaagtggca tggcgccagc cccgcctgcg aacaggccga
     1681 tgcgggtcga cgcgagcagg cgcggccatc gtcgccggtg tcgttcctga ccattctgct
     1741 ggcggcatcc ggtgtcatca ttctcctcgc ggtaacaaac gagatcaccc aaaacatcgc
     1801 tcccgttcca ttcctgtgga tactgccgct ggttacgtac ctgttgacct acattatctg
     1861 cttcagcggc gagcgctggt acgatcgcgc gatctggggt tcgctgttcg tcgtatcggc
     1921 cagcagcctg gttatcctgc atttcttcgg cacgtccttt cctatcgtgc cggtgatcgt
     1981 tgcctacctg ctcgtacagt tctgcgccag catagtctgc catgccgagc tgtaccggct
     2041 caggcccgaa ccttcacggc tgaccggctg gtacctgcag attgcgctgg gcggcgcgat
     2101 cggcggcgcg ttcgtctcgg tggtcgcgcc gctggctttc tcacgatact gggaagcgct
     2161 ggtcggtgtt tacctggtct acgtcgtcct cgggatcgtg gttcttcgtg agagccgcga
     2221 accgcctgaa aagaagccgt tccatgttgt gtctgcaatc cagcgcaagc tggagcggtg
     2281 ggcgctgcgc ctgtttgcgc tcggttgggt caccggcctt ttcctgctac ctgcggtcgt
     2341 cctgtcgctc gacaagctgc gtgtccgata cgacgttacg agttcaagga acttctacgg
     2401 cctgttgaac gtgcgggacg tggtcaccga cggtgaggcc cggcgattgc tcgtggacgg
     2461 aactacgatc cacggtttcc agctgctgga cgagaagcgg caggatgttc ccaccagcta
     2521 ctacggcgcc aacacaggca ttgcgatggt catggaaaac gttgaacggc cggtaaccgg
     2581 attgaacgtc ggcattgtcg ggttaggcac gggcacgctc gctgtctacg ggaaacccaa
     2641 cgacaggttt cgcttttacg aactgaaccc cgcagtggtg gacgtcgcga atacacattt
     2701 ctcattcctg cgccagtcgc cggcggacgt cgacatcgtc acgggcgacg gacgcgtggc
     2761 gatggagcgt gaactcgagc ttgaaggcag tcgggagtac gacttgctcg tcattgacgc
     2821 gttcaacagc gacgccatcc cggtgcacct gctcactttg gaggcgctgg aactttattg
     2881 gtcacacctg aagccggatg gagttctcgc tcttcacctc acgaacaact acctcgacct
     2941 ggttccggtc gcgagtaacc tgggggccag cctgggcaag gaagtcctgt tcgtgactac
     3001 gccgcgtgat gtcggcgtga gttcggccgc ggattgggta ctactcgcag aagacccggc
     3061 cctgttcgcg ccgggtacgg gggagcatgc cggaagcgag ttgaccgtgc cggggcagga
     3121 acgcctctgg acggatgact acagtaatct cctcggcacg ttgaagcgtg gcaggaaata
     3181 gccccgggct gtgcccgccg gcacagcccg tttacatcaa tgccgccggc ggcgcactac
     3241 cggagatcag gatccctgtc gaccgggtcc cgggtggtcc gggccgaagt gcggtccacg
     3301 ggatcacgag tcgtatcgcg cgtcgtcgtg gtagttgtcc tgtgggtggt cgccgcgtcc
     3361 cgctgaacag gaaccctgtc ccgcgacagg aacgacaatg cgatcatgcc tgcgccgaga
     3421 aacaggtgca gccagtcatc ggcattgttg agcggcacga agttcgcttc cgaagcctcg
     3481 ttgacaacca atccgtagag ccacaggaga aggtagagga tgccgcctac catcaggaac
     3541 ctgcgagcgc ctggcgatgt cttcgacatc gtgattccag ctacgccaaa ggccaggtgg
     3601 actatgttgt gcagcaccga aacctggaag atgccgagca acattgcttg cgactcgtgt
     3661 ccggcaaatt ccatcgcgct gtagttggtc gtgatgccgg ggatgaaacc taagatacca
     3721 acgatcaaaa acacgacgcc ggtaatgaag gctgcttgct gtaccggtgt gcgtgtcgtt
     3781 gctcgcgtgg tatcacgcgc tatcgttctg gccatttgta tctccttaac aaacgtagtg
     3841 ccccaatcac cggtagattc agtatagcga aaacgtgtat cgcaaatgtg tgtctttgtg
     3901 cttcaggaag cgtgtctgtt ccggaacttc ccgctctttc gcgacacgcg gtcagcacgc
     3961 gtttattcaa ggcctgattg taattcgtac aacggaagcg cgggtcacca caggtttccc
     4021 gacgagtggg gcgctggcgc taacgacgca cggcgcggga aaccgggtat gatcgttcaa
     4081 cgcacgcagc gcccgctgca gccgcacttg agaagagaga acgaatgccc ggaacgctga
     4141 ccatcacatg ctgaaaatca ttcgcctgcc gttcccgtgg gcactctttg taatggccgg
     4201 caccgtttcc gcctgcgcgt acatgcccaa taacgatccg ctggctattt cagttgcggg
     4261 agtagagtca ttgccgggcg agggaatgga aatgcgtctt gcagtaaaag tccgtatcca
     4321 gaatccgaac gacctgccgg tcgactacac cggtgctgcg ctgacgctcg acctcaatgg
     4381 caggaagctc gcgagcggtg tgatgggcga atcgggtacc gtgccgcgct atggtgaggt
     4441 cgtgatggtg atacccgtga ccatatcggc gttcgacatg gcgcgccagg tgttcggcgt
     4501 cgccaacgca tcaggccaga gcgacgtgca ttatcgcgta cgcggcaaac tcgaggcagg
     4561 gctgttcggc acacgacgtt tcagcgacga aggaatgttc cagctcgacc agccggttga
     4621 accaggttca tgaaggctgt cggctgcgga gcaccattct cgtaccgcgg cggctgtggc
     4681 cggtgcgcgg caccggcgat tcgctcttga gtcaacagga tcgaatcaag tgggacgaga
     4741 agtaccgtgc cggcgcgtac gccgggcggg atcacccgac ccggttgctt gccgaatggg
     4801 taccgcaggc gaatcgcggc cgtgcactgg acgtggcttg cggtgcgggg agaaacagcg
     4861 tgttcctggc ggtccagggt tatctggtcg acgcggtcga catctcggcg gccggactcg
     4921 aatgcgcacg caggagcgcc gagacgcgcg gagttcacgt gcactggatc gaagccgacc
     4981 ttgaaacagg cgcggcatcg gc
//
LOCUS       DASUOM010000214         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1115424,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000214 DASUOM010000000
VERSION     DASUOM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115424"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFY27_04460"
     CDS             <1..439
                     /locus_tag="VFY27_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX5764796.1"
                     /translation="VLAVPVSVITSRSNVGEAAGRLGLFLTPEEVAPSGVVRRFREIV
                     EAAEDNDAAARAGQEQPLWVAALLNPGIYNLHRKLLPAEEPTRRQRHQLDGLMYTLID
                     QSEDALDARERRAMITNATCLKRIHIGLWSELPLERLSAQQPA"
     gene            525..1907
                     /locus_tag="VFY27_04465"
     CDS             525..1907
                     /locus_tag="VFY27_04465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764797.1"
                     /translation="MNLTFLGAAGAVTGSCYLLQTSKHRLLLECGQVQGSAAEEALNR
                     SPLPVPWDSIDAVVLSHAHVDHSGRLPLLHREGYDGLIYTHDATRALCGVMLRDSGYL
                     HEKDAEWENQKRRRKGLPLVKPLYTQNDAERVMRQFEGLEYSKRQAILPGIELRLNDA
                     GHILGSAIVELWLEDKGQRCKLVFSGDLGYASSPVMNDPAVIREADLVMLESTYGDRN
                     HRSFEATLDELQGIFRAAADSGGNVLIPAFAVGRTQDLLYLMAEHYGDWRLDQWQVFL
                     DSPMAIEATGIYSQHRHLYPAELFREGQHWPVLPNFAATASSEESVSLNRIESGAIII
                     AGSGMCTGGRIRHHLKHNVWRPQCHVVMIGYQAHGTLGRRLVDKAPRIRLWGETIQVN
                     ATVHTVGGLSAHADQDGLLDWYGNFEARPPVYLVHGEDKPRLALADELQKRYGVEAHR
                     PKLDEVVELV"
     gene            complement(1914..2273)
                     /gene="cutA"
                     /locus_tag="VFY27_04470"
     CDS             complement(1914..2273)
                     /gene="cutA"
                     /locus_tag="VFY27_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5764798.1"
                     /translation="MNVPTENAGTFMNESATLVMTTVGSEELARTLAARLLDERLAAC
                     VQEIRINSHYRWNGDLHYEPEILLLVKTSAKAAPAAMRLIEDIHSYDVPEIIALPVTA
                     GLPAYLEWVAGEADGKR"
BASE COUNT          449 a          764 c          811 g          430 t
ORIGIN      
        1 ggtactggcg gtgccggtgt ccgtcatcac gagtcgctcc aacgtaggtg aggcggccgg
       61 gcggctgggc ctgttcctga ccccggaaga agttgcgcct tccggcgtgg tgcggcggtt
      121 tcgcgaaatc gtcgaggcgg cggaggataa cgatgccgcc gcgagagcag gccaggaaca
      181 gccgctgtgg gtcgcggcgc tcctgaaccc gggaatctac aacctgcacc gcaaactgct
      241 gccggcggag gaaccgacgc ggcgccagcg ccaccagctg gacgggctga tgtataccct
      301 catcgaccag agcgaggacg ctctcgatgc cagggagcgc cgcgccatga tcaccaacgc
      361 gacctgcctg aagcgtatcc acatcgggct ctggagcgag ctgccactgg agcggctgtc
      421 cgcgcagcag ccagcctgat cgatcgcgtc cgtgccctcg cggggcacag ccctgcgtcc
      481 tgtggcactc tgtgctgtac acaagaacat ttgaagggta atagatgaat ctcacctttc
      541 ttggcgccgc gggggcggtg accggatcct gttacctgct gcagacgtcg aagcacaggc
      601 tgctgctcga atgcggccag gtccagggca gcgcggccga ggaggctctc aacaggagcc
      661 ctttgccggt gccgtgggac agcatcgatg ccgtcgtcct gagccatgcc cacgtcgatc
      721 actcgggcag gctgccgctc ctgcacaggg aaggctacga cggcttaatc tatacccatg
      781 atgcaacccg cgcgctctgc ggcgtcatgc tgcgcgattc gggctacctt catgagaagg
      841 atgcagaatg ggagaaccag aaacgccgca ggaaaggact gccactcgtc aagccgcttt
      901 acacgcaaaa cgatgctgaa cgcgtcatgc gccagttcga agggctcgag tattcgaagc
      961 gccaggcaat actccccggc atcgagctac ggctgaacga tgcggggcac atcctcggat
     1021 cggcaatcgt cgagttgtgg ctggaggaca aggggcagcg ctgcaagctc gtcttcagcg
     1081 gggacctcgg gtatgcgtcc tcgccggtga tgaacgaccc cgccgtgatc agggaggcgg
     1141 atctcgtgat gctggagagc acctacggcg atcgcaatca ccgttcgttc gaggcgacgc
     1201 tcgatgagct gcagggcatt tttcgcgccg ccgcagacag cggcggcaac gtcctgatcc
     1261 cggcgttcgc agtcgggcgg acgcaggacc tgctctacct catggcggag cattacgggg
     1321 actggaggct cgatcagtgg caggtgttcc ttgacagtcc gatggccatc gaggcgaccg
     1381 gcatctattc acagcaccgt cacctgtacc cggccgagct gttccgcgaa ggccagcact
     1441 ggccggtgtt gccgaatttt gcggcgaccg ccagctcgga agaatcggtg tcgctgaacc
     1501 ggatcgagtc gggtgcgatc attattgcgg gcagcgggat gtgcaccggc gggcgcatcc
     1561 ggcatcacct gaagcacaac gtgtggcggc cccagtgcca cgtcgttatg atcggttacc
     1621 aggcacacgg cacgctggga agaaggctgg tcgataaggc gccgcgcatc cgtttgtggg
     1681 gtgaaacgat acaggttaat gcgacagtgc acacggtcgg cgggctctcg gcgcacgctg
     1741 accaggatgg cttgctcgac tggtacggca atttcgaggc gcggccgccg gtgtacctcg
     1801 tgcacggcga ggacaagccg cggcttgcgc ttgccgacga actgcagaag cgctacggcg
     1861 tcgaggccca ccggccgaag ctcgacgagg tggtcgagct ggtttgagcc cggctagcgc
     1921 tttccgtcgg cttcgccggc cacccactcc aggtaggcag gcaaacccgc ggtgacaggc
     1981 aaggcgatga tttccggaac atcgtaactg tggatgtcct cgatcaggcg catggcagcg
     2041 ggcgcggcct tcgccgaggt tttcaccagc agcaggatct cgggctcgta atgcaggtcg
     2101 ccattccagc ggtaatggct gttgatgcgt atctcctgca cacaggccgc gagccgctca
     2161 tcgagcagtc gggccgcgag ggtccttgcg agttcttcgc tgccgacagt ggtcatcaca
     2221 agcgtggcgg attcgttcat gaaagtgccc gcgttttcgg tggggacgtt cattctccgg
     2281 cgccccatcc tgctgcgcaa atccgcccgg cggccgctac gtgaccttaa agaacgtgcg
     2341 gcctgacggc ggttcgcgca atccatcccc cgaacccgcc caacagttgc cggagtgcct
     2401 gcgaggtacg tttgtcgacc aggtgcccgc catcgaacag ctctcccgcc ccgg
//
LOCUS       DASUOM010000215         7222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_651766,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000215 DASUOM010000000
VERSION     DASUOM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651766"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /gene="dapE"
                     /locus_tag="VFY27_04475"
     CDS             complement(<1..1093)
                     /gene="dapE"
                     /locus_tag="VFY27_04475"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5764799.1"
                     /translation="MTDTAFTAGEPRPDDAALDLLCDLIRRRSITPEDAGCLELIAER
                     LQSAGFTCEFMPFGRVSNLWARRGTGRPLLCFAGHTDVVPPGPMENWTSDPFEPNLRD
                     GRIYGRGAADMKSGLAAMVTAALQFVAAHPRHSGSMAFLLTSDEEGRAREGTKKVIEA
                     LSARSERIDWCVLGEPSSREHLGDTVRIGRRGSLSAIATVRGIEGHVAYPEQVDNPIR
                     RFAPVLEELHATVWDEGNEFFPPTSFQVISIASDGGAPNVTPAELKARFNFRYSTVWT
                     SETLKEKVEKLFSAHDIDCTLDWHLSGEPFLTSEGPLTEAVVQAVYEQSGLRPELSTG
                     GGTSDGRFISPAGAAVVELGPVNAFIHKPD"
     gene            complement(1298..1648)
                     /locus_tag="VFY27_04480"
     CDS             complement(1298..1648)
                     /locus_tag="VFY27_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEX5764800.1"
                     /translation="MLTIYGIKSCSTCRDAKKWLDAQGIAYRFHDLREDGIDIQTLER
                     WADSVEWEKLLNKSSLTWRKLPDVDRSGMTRNRALAAMIEHPTLVKRPVLEKGRQVAL
                     GFSARQYAEMLGSN"
     gene            complement(1700..2530)
                     /gene="dapD"
                     /locus_tag="VFY27_04485"
     CDS             complement(1700..2530)
                     /gene="dapD"
                     /locus_tag="VFY27_04485"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019674888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX5764801.1"
                     /translation="MIAAIEAGFDARGDASRGSPQPDLETAVAEALALLNSGEGRVAE
                     KAGASWRVNPWLKKAVLLSFRLRENRVIAGAHSNFYDKVELKYGDYGPADFERDRVRV
                     VPDAIVRYGAFVARDVVLMPSYVNIGANIGSGTMIDTWATVGSCAQIGRNVHISGGVG
                     IGGVLEPVQAAPTIIEDDCFIGARSEIVEGVIVETGSVISMGVFIGQSTKIFNRETGE
                     VSYGRVPAGSVVVPGNLPSSDGTHSLYCAVIVKQVDAKTRSRTSLNDLLRFAGEELRL
                     "
     gene            complement(2542..5244)
                     /gene="glnD"
                     /locus_tag="VFY27_04490"
     CDS             complement(2542..5244)
                     /gene="glnD"
                     /locus_tag="VFY27_04490"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5764802.1"
                     /translation="MTPVPLAGVAPSPHAEPAGDAASDPALRLLLRLLPDAYAGVSTC
                     RRALEEAGRNLEERFRKGEPVGKLVRLRALVVDRLLTYLWRQHAAAIEGSVALVAVGG
                     YGRGELHPSSDVDIMLLRDDSLPAGAEAAISAFITALWDAGLEIGHSVRTVGQCRHEA
                     EADVTVTTTLMEARLLAGPERLLDDLAAAVGPDQVWPSAEFFAAKRKEQRARHLRYDD
                     TAYKLEPNVKGSPGGLRDIQMIGWVAKRHFGARTLDELVDHGFLTPDQLRALYDGQAF
                     LWRIRYALHLLTGRREDRLLFDHQTRLAQMLGYEDASYTLAVEQLMQRYYRTVMELSR
                     LNEMLLQLFEQAILMDPYAEPEPLNERFEVKNGYLQTVHEDVFRQEPSALLELFLLLQ
                     ENEHIRGVSAHTVGEIRRNLYLIDEEFRQNPRNHRLFLSILRAPVGVSRQLRRMNLYG
                     VLGLYIPSFGRIVGRMQYDLFHAYTVDEHTLFVVSNLRRFALSRFDHEYPHCSPLMQS
                     FDKPEIVYLAGLFHDIAKGRGGDHSELGAVDAEAFCLEHGMSRYEARTVAWLVRNHLL
                     LSTTAQKKDISDPAVINEFAAIVSDKTHLDYLYVLTVADVRGTNPKLWNSWKATLFRD
                     LYDATARALRRGLENPIDREYLISETQTSARELLRQSGMSEAQRQEIWRSFTEDYFLR
                     YRSEEIAWHTRILAGAGEGAAHGLVDVRKQTGGDGMEAVLYTLKTRRTFAHATAAIDE
                     LGMTIVDARVVPIRNGLSLDTFIFMELDRRIEVDDLRLRKVRQALANVLETSDGSIVK
                     VTRRAPRQVRMFPTRTSVEFDEDAAHGRTVMELVAGDRPGLLSKVGQVLIDLGVEIDT
                     AKIMTIGERAEDVFYIVDGNGKPLSAELRETLRQRLVSTLDTRE"
     gene            complement(5264..6037)
                     /gene="map"
                     /locus_tag="VFY27_04495"
     CDS             complement(5264..6037)
                     /gene="map"
                     /locus_tag="VFY27_04495"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5764803.1"
                     /translation="MHVTIKTPEEQEKMRVAGRLAADVLDMIGEHVVPGVTTDELDRI
                     CHRYIVETQQAVPAPLNYKGFPKSICTSVNHVVCHGIPGDRKLKSGDVVNIDVTVIKD
                     GYHGDTSRMYFVGKPGVQAERLSKVAYEAMWLGIEQLGPGKHLGDIGEAIQTHVERSR
                     FSVVREYCGHGIGRVFHEDPQVLHYGKAGSGMELKAGMTLTVEPMVNAGKRHVRLLPD
                     GWTVVTRDHSLSAQWEHTVLVTDSGYEVLTLPAAQRPAA"
     gene            6480..>7222
                     /gene="rpsB"
                     /locus_tag="VFY27_04500"
     CDS             6480..>7222
                     /gene="rpsB"
                     /locus_tag="VFY27_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5764804.1"
                     /translation="MADVTMRQMLEAGVHFGHQTRYWNPKMAPYIFGERNKIHIINLE
                     KSLPLAREAYAFVKAVVADHGSVLFVGTKRSARDSMRAQAERAGMPFVTYRWLGGMLT
                     NYKTIRQSVKRLHDLEQMAEEGSLDRLTKKEALGLSREREKLERSLGGIKDMSSLPDV
                     LFVVDVDHEDIAVREARKLGIPVVAVVDTNCSPDGVEYVIPGNDDAMRAIELYATLIA
                     DAVLDGKASLPEVALGEDDFVELDEEGRPR"
BASE COUNT         1317 a         2308 c         2222 g         1375 t
ORIGIN      
        1 cgtccggctt gtgaatgaac gcgttaaccg ggcccagttc caccaccgcc gcgccggccg
       61 gtgaaatgaa ccgcccgtcg gacgtgcctc cgccggtcga cagttccgga cgcagccccg
      121 actgttcgta gacagcctga acgaccgcct cggtgagcgg gccctctgaa gtgaggaaag
      181 gctcgcccga cagatgccag tccagggtgc agtcgatatc gtgagcgctg aagagcttct
      241 ccaccttttc tttaagcgtc tcgctggtcc ataccgtgga gtagcggaag ttaaagcggg
      301 ctttcaattc ggccggtgta acgttcggtg cgccaccgtc gctcgcgatg ctgatcacct
      361 ggaagctggt cggcgggaag aattcgttgc cttcatccca tacggtggca tgcagttctt
      421 ccaggaccgg agcgaagcgc cggatgggat tgtcgacctg ctcgggatac gcaacatggc
      481 cttcgatgcc gcgtaccgtc gctatcgcac ttagcgaacc gcgccgtccg atgcgcaccg
      541 tatccccgag atgctcgcga ctcgatggct ccccgagcac gcaccagtcg atcctttcgc
      601 tgcgcgccga cagcgcttcg atgactttct tcgtcccctc gcgcgcccgg ccttcttcgt
      661 cgctggtcag gaggaaggcc atgctgccgc tgtgccgggg atgggcggcg acgaactgca
      721 gcgccgctgt caccatggca gcgagtccgc tcttcatgtc cgccgcgccg cgtccgtata
      781 tcctgccgtc gcgcaggttg ggctcgaacg ggtcggaagt ccagttttcc ataggcccgg
      841 gggggacgac atccgtgtgg ccggcaaagc aaagcaatgg acggccggtg ccacgccttg
      901 cccagaggtt gctgaccctt ccgaatggca tgaactcgca cgtgaaacca gccgattgca
      961 gccgctccgc aataagctcg aggcagccgg cgtcctccgg agtgatggag cgacggcgga
     1021 tcaggtcgca gaggaggtcc agcgcggcat cgtcgggacg gggctcgccg gcagtaaacg
     1081 cggtatctgt cattgttgtt gaggtatcca tcccaagcta ttaattaatc gtcgcttatt
     1141 gcttgctgcg cttgccgtat tcgctgccgg caaaggtctg cttcgtccgg cttcccccag
     1201 tcttcgtaga ggctcgcccc cattgcatcg cgcgccgggt acgcagggag cgacgatgag
     1261 acccaggcgg acgcgcggga aaagtgggct aagcgcgtca attactaccc agcatttcgg
     1321 cgtactgtcg cgcggaaaag ccgagcgcga cctgcctgcc cttttcgaga accggccgct
     1381 tcacgagagt cgggtgctcg atcatcgccg ccagcgcgcg atttcgcgtc atgccgctgc
     1441 ggtcgacgtc cggcagcttg cgccatgtga ggctcgactt attcagcagc ttttcccatt
     1501 ccacgctatc cgcccagcgc tccagcgtct ggatatcgat tccatcttca cgaaggtcgt
     1561 ggaaacgata agcgatgcct tgcgcgtcca gccatttttt tgcgtcacgg caggtactgc
     1621 agctcttgat tccgtagatc gtcagcataa tcgtgaagct ccgcgcaaaa cagatcaaga
     1681 caactgtagg agcgggctcc tacagtcgca gctcctcgcc ggcgaagcgc agcaggtcgt
     1741 tcaggctcgt tcggctgcgg gttttcgcat cgacctgttt cacgatgacg gcgcaataga
     1801 gagaatgcgt tccgtcgctc gagggaaggt tacccggaac cacgacggat cccgccggca
     1861 cccggccgta gctgacttca ccagtttccc ggttaaagat cttcgtgctt tggccgatga
     1921 agacgcccat cgagatgacg gaacctgttt cgacgatcac gccctccacg atttcggagc
     1981 gggccccgat gaaacagtcg tcttcgatta tcgtcggcgc ggcctgaacg ggttcgagaa
     2041 cgccgccgat gccgacgccg cccgaaatgt gcacgttgcg cccgatctgc gcgcagctgc
     2101 cgacggtcgc ccaggtatcg atcatggtgc cgctgccgat gttcgcaccg atgttgacgt
     2161 agctcggcat cagcacgacg tcgcgtgcaa cgaacgcgcc gtagcgcacg atcgcgtcgg
     2221 gaacgacgcg cacgcggtcg cgctcgaagt cggcaggccc gtagtcgccg tacttcagtt
     2281 ccaccttgtc atagaaattg ctgtgggcgc cggcgatgac cctgttctcc cgcaggcgaa
     2341 aactcagcag tacagctttc ttcagccagg gattgacccg ccaggaagcg ccggcttttt
     2401 cggcaacccg gccctcaccg gagttgagca gtgcgagcgc ttccgcgacg gcggtttcga
     2461 ggtccggctg agggctgcca cgtgaagcat cgccgcgcgc gtcgaaaccc gcctcgatgg
     2521 cggcaatcat cgcctccctg ttcactcccg cgtgtccagc gtggagacca ggcgctgcct
     2581 caacgtctcg cgtagctccg cggacaatgg tttgccgttc ccgtcgacga tgtagaacac
     2641 gtcttccgcg cgctcgccga tagtcatgat tttcgccgta tcgatttcga cgcctaagtc
     2701 gatgaggacc tgccccacct tgctcagcag acccggccgg tcccccgcca ccagttccat
     2761 cacggtgcgg ccgtgcgcag cgtcctcgtc gaattcgacg gaggtacggg tcgggaacat
     2821 gcggacctgc cgcggggcgc ggcgggtgac cttgacgatg ctgccgtcgg acgtctccag
     2881 cacattcgcc agcgcctgcc gcactttccg caggcgcagg tcgtctactt cgatgcggcg
     2941 gtcgagttcc atgaatataa aggtgtcgag gctcaacccg ttcctgatcg gcacgacgcg
     3001 cgcatcgacg atcgtcattc ccagttcgtc gatcgccgcg gtcgcatggg caaacgtgcg
     3061 cctggtcttg agcgtgtaaa ggacggcctc catcccgtcg ccgccggttt gcttgcgcac
     3121 gtcgaccagg ccgtgcgcgg cgccttcgcc ggcaccggcg agtatccgcg tgtgccaggc
     3181 aatctcctcg ctgcgataac ggaggaaata gtcctcggtg aaggagcgcc agatttcctg
     3241 ccgctgcgcc tccgacatgc cggactggcg cagcaattct cgcgccgacg tctgcgtttc
     3301 cgaaatcagg tattcgcggt cgatcgggtt ctcgaggccc cgacgcagcg cccgcgcggt
     3361 tgcatcgtac aggtcgcgaa acagtgtcgc tttccatgag ttccagagct tgggattggt
     3421 gcccctgaca tcggcgacgg tcaggacgta aaggtagtcg aggtgcgtct tgtcgctgac
     3481 gatggcggcg aactcgttga tgacggcggg atcggaaatg tcttttttct gcgccgtagt
     3541 cgaaaggagc aggtggttgc ggaccagcca cgctaccgtt cgggcctcgt accggctcat
     3601 gccgtgttcc aggcagaatg cttcggcgtc gacggcaccg agttccgaat gatccccgcc
     3661 gcggcccttg gcgatgtcgt gaaacaggcc cgcgagatac acgatctcgg gcttgtcgaa
     3721 cgattgcatc aacgggctgc agtgcggata ttcgtggtca aagcgcgaaa gagcaaagcg
     3781 gcgcaggttg cttacgacga acagcgtgtg ttcatcgacg gtataggcat gaaaaaggtc
     3841 gtactgcatg cggccgacga tgcgtccgaa cgacggaatg tagagaccca gcaccccgta
     3901 aagattcatg cggcgcagct gccggctgac acccaccggc gcccggagga tgcttaagaa
     3961 tagccggtgg ttgcgcgggt tctggcggaa ctcctcgtcg atgaggtaaa ggttgcggcg
     4021 gatctcgcca acggtgtgcg cggagacgcc tcggatgtgc tcgttttcct gcaacaggag
     4081 gaacagttcg agcaatgccg agggttcctg ccggaacacg tcttcgtgca ccgtttgcag
     4141 gtacccgttt ttcacctcga agcgctcgtt cagcggttcc ggttcagcgt acggatccat
     4201 caatatcgcc tgctcgaaaa gctgcagcag catttcgttc agacggctca gttccatcac
     4261 cgtgcggtaa taacgctgca tgagctgttc gaccgcgagt gtgtagctcg catcttcgta
     4321 acccagcatt tgggcaagcc gcgtctggtg atcgaacagg aggcggtcct cgcgccttcc
     4381 ggtcagcagg tgcagggcat aacggatccg ccacaggaat gcctggccgt cgtataacgc
     4441 ccgcagctgg tcgggtgtca ggaagccgtg atccacgagc tcgtcgagcg tgcgggcgcc
     4501 gaaatgccgt ttcgccaccc agccgatcat ctggatatcc cgcagcccgc ccggactgcc
     4561 tttgacgttc ggctccagct tgtaggcggt gtcgtcgtag cgcaggtgcc gcgctcgttg
     4621 ctctttgcgc ttcgccgcaa aaaattcggc cgacggccat acctggtcag gcccgacagc
     4681 ggcggcaagg tcatcgagca gccgctccgg gccagccagc aatcgagctt ccatcaatgt
     4741 cgtcgtgacc gtcacgtccg cttccgcctc gtgacggcac tgcccgaccg tccggacgct
     4801 gtgcccgatc tcgagtccgg cgtcccacag cgcggtaatg aaggcggaga tcgcagcttc
     4861 cgccccggca ggcaggctgt catcgcgcaa caacatgatg tcgacatcgg atgacgggtg
     4921 cagctcgcca cgaccgtagc cgcctaccgc caccagcgcc acgctgcctt cgatcgcggc
     4981 ggcgtgctgg cgccacaggt aagtgagcag ccgatcgacg accagcgccc gcagccgcac
     5041 cagcttcccg acgggctccc ccttgcggaa gcgctcctcc aggttgcggc ccgcttcctc
     5101 cagggcccgc cggcacgtgc tgactcccgc ataagcgtcg ggcagcagtc gaagcagcaa
     5161 cctgagtgcc ggatcgctcg ctgcatcgcc tgccggctct gcatgcgggg agggggcaac
     5221 accggcgagg ggaacgggag tcatgaatat cacgtgtcca gggtcacgcg gcgggacgct
     5281 gcgcggccgg aagcgtcagc acctcgtacc cgctgtcggt gacgagcacc gtgtgttccc
     5341 actgcgccga cagcgagtgg tctcgcgtca ccacggtcca gccgtccggc agcaaccgca
     5401 cgtgccgctt gccggcattg accatcggct cgaccgtcag cgtcatccct gccttcagtt
     5461 ccatgccgct gcctgccttg ccgtagtgca gcacctgcgg atcctcgtga aaaacccggc
     5521 cgatcccgtg cccgcaatac tcgcgcacga ctgaaaagcg gctgcgctcg acgtgcgtct
     5581 gtatagcctc gccgatatcg ccgaggtgct tgccggggcc cagttgctcg atcccgagcc
     5641 acatcgcctc ataagccacc ttcgaaagcc gctccgcctg cacccccggc ttgccgacga
     5701 agtacatccg gctcgtgtcc ccgtgatagc cgtccttgat cacagtgaca tcgatattga
     5761 ccacgtcgcc cgatttcagc ttcctgtccc ccggaatgcc gtggcacacc acgtgattca
     5821 cggaggtgca gatcgattta gggaagccct tgtagttcag gggagccggg accgcctgct
     5881 gcgtctccac gatgtaccgg tggcagatac ggtccagctc atcggtggtc acgcccggca
     5941 cgacatgctc gccgatcata tccagtacat cggcggccag ccgccccgca acccgcatct
     6001 tttcctgctc ttcgggagtt ttgatcgtca catgcatatg agtttcaatg ccgctcaagc
     6061 agagcaagca ggaatccagt agtttcgccg gcccggggca gcattatcca tgaacggtca
     6121 ccgtcggtgg ttagcggcgc cttggctggg gggagggctt gtcaggaacg ccgtgaaccc
     6181 atccatgggg gctctgcgca aacatccctg tttgcgacag tcctgacaag ccctcccccc
     6241 aaccaagacc ccgcatgaga gcgctgcggc gttcatggat aatgcttggg tgatctgatc
     6301 gcttgaccgt gagcggataa tccgcacaga tccgcgttgt tgagccaaac cgggtatggt
     6361 ataaagcgcc cgccccagag gcaattaatc cacacgtaca ccgacacata ccgcagggtg
     6421 ccccacctga aaaggggttg cggcatgggg tgtacggagg ctgaacccaa ggagacaaca
     6481 tggcagacgt aaccatgcgc cagatgcttg aggctggcgt gcatttcggt caccagaccc
     6541 gttactggaa tccgaagatg gcaccctaca tcttcggcga acgtaacaaa atccatatca
     6601 tcaatctaga gaagagcctg ccgctcgcgc gtgaggccta tgccttcgtg aaagcggtcg
     6661 tcgccgatca cgggtcggtg ctcttcgtcg gcacgaagcg ctccgcgcgc gattcgatgc
     6721 gcgcccaggc agagcgcgcc gggatgccgt tcgtcacgta ccggtggctc ggcggcatgc
     6781 tgacaaacta caagaccatc cggcagtcgg taaagcgcct gcacgatctc gagcagatgg
     6841 ccgaggaagg cagcctggac cggctgacga aaaaggaagc gctcgggctc tcgcgcgaac
     6901 gcgaaaagct cgagcgatcg ctgggcggca tcaaggatat gtcgtcgctg ccggacgtgc
     6961 tgttcgtcgt cgatgtcgat cacgaagaca tcgcggtgcg cgaagcgcgg aagctcggca
     7021 ttcccgtggt tgcggtcgtg gatacgaact gctcgccgga tggcgtggaa tacgtcattc
     7081 cggggaacga cgacgcgatg cgggcaatcg agctttacgc gaccctgatt gcggacgcgg
     7141 tgctggacgg caaggcctcg ctccccgagg tcgccctcgg tgaggacgac ttcgtcgaac
     7201 tcgatgagga aggcaggccg cg
//
LOCUS       DASUOM010000216         5463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1345751,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000216 DASUOM010000000
VERSION     DASUOM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345751"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /gene="bglX"
                     /locus_tag="VFY27_04505"
     CDS             <1..1794
                     /gene="bglX"
                     /locus_tag="VFY27_04505"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEX5764805.1"
                     /translation="IARDPRWGRISEGAGEDVYLGSEIAQARVRGFQGEDLGAVDTIL
                     ATAKHYAAYGQAQAGRDYHTTDMSLRELWDTYLPPFRAASEAGVATYMTAFNDLNGVP
                     ATANSYLLTEILRTRWSFDGFVVSDYTSVNELVPHGFARDEKHAAELAINAGVDMDMQ
                     GASFMKHLPQLVKEGRVDVQTIDAAVQRILEMKYRLGLFEDPYRYSNEAREAAAIYAE
                     EHLEAARDMARKSIVLLKNERDLLPLSADVSLAVIGPLADSEPDMIGSWSAAGEREDR
                     PVTLLEGIRERVGEQADIHFEEGASYRFGDEDERAGFDAALAAAGRADVIIAAMGERW
                     DMTGEAASRTSLGLPGNQLALLKELRKLGKPVVLVLMNGRPLALEWADENIHAILETW
                     YAGTEGGHAIADVLFGDYNPSGKLPVTFPRNVGQVPIYYNMKNTGRPYTAGAAEQKYK
                     SRYLATPNSPLYPFGYGLSYTGFTYSDLALDRETMREGESITVSVRVTNSGEVDGEDV
                     VQLYTRDLVGSVTRPVKELKGFRKISLDAGASRVVEFTLDPSDLAFHRQDMSYGAEAG
                     KFKVFVGGNSEELLEAGFRLTDDVRLSDAPP"
     gene            1940..3037
                     /locus_tag="VFY27_04510"
     CDS             1940..3037
                     /locus_tag="VFY27_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5764806.1"
                     /translation="MKAPKPTSRDIAFEAGVSQATVSRALRNSPLVRPETRQRIHDIA
                     AQLNYRVDKHAAGLRTRQSRTIALLLFEDPTSDDSQINPFFLSMLGSITRAAASIDYD
                     VLVSFQQLSNHWHGRYEASHRADGIILLGYGDYVTYGEKLKALAEANAHFIIWGPIID
                     EQPGHYLGCDNIGGALRATQHLVGLGRRHIAFLGGASEHCPEFEHRHRGYLQALRNAA
                     IAPDPDLRVDADNQESSGYAAAAALLKTGKQVDGIFAASDLIAIGAIRALQDAGLDVP
                     RNVSVVGFDDIPAASYVNPGLTTVHQDTARAGELLVTNLIKSIEGERVESSLLPPTLV
                     IRQSCGAVAESNGRQALTSDPRTASPRLLRP"
     gene            complement(2973..4508)
                     /locus_tag="VFY27_04515"
     CDS             complement(2973..4508)
                     /locus_tag="VFY27_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5764807.1"
                     /translation="MGEIAEQITLRTKPRLGFWQIWNMSFGFLGIQFGFALQNANVSR
                     IFQTLGASIDDLPILWVAAPVTGLLVQPLIGHMSDRTWGRFGRRRPYFLVGAILASLA
                     LFVMPNSPALWVAAGMLWIMDASINVSMEPFRAFVGDMLPDEQRTNGFAMQSFFIGLG
                     AVVASALPWMMTNWFDISNTAPAGVIPESVKLSFYFGGAVFLAAVLWTVLRTREYSPA
                     ELDAFEAARSQADVSKRDAGRPAAKYTIGGLIWILGGGALLVWIVGSDLDKQLYVLAL
                     GAALFGAIQVLSGAMQSRNRTSNGLYSIVHDLFHMPRVMRQLAVVQFFSWFSLFAMWI
                     YGTAAVTSHHYGTLDVTSQLYNDGADWVGVLFAAYNGFAALAAIMIPFVAGWLGCRRC
                     HAVNLSLGGIGLLSFFFVREPSMLLLPMIGVGIAWASILSLPYVLLSSILPARKMGVY
                     MGIFNFFIVIPQLVAASILGLLLREFFEGRSIFGLVTGGAFMVAAGVATLFVDRSSEP
                     GGH"
     gene            complement(4630..>5463)
                     /locus_tag="VFY27_04520"
     CDS             complement(4630..>5463)
                     /locus_tag="VFY27_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEX5764808.1"
                     /translation="LGVRKVKTGYVADAGELKRVDENGIQRFEWHDGQFSVNHHLRAL
                     TEAAKHRISLNPHEPVKDTGLRRTYPNWLSREGARGQEYNAWGEPPNPPEHTAILPYT
                     RMLSGPMDFTPGVFDLTFKGANSPFRVKTTLAKQLALYVVLYSPIQMAADLPENYEQR
                     PDAFQFIVDVPTDWEQSVAVAGEVGDFVAFARKERDGADWYLGALTDENARSLSLPLS
                     FLDESTEYVAEIYRDGDDAHWYTAPYELVIEERGFSRDDVLDLRLAAGGGAAIRFRAT
                     R"
BASE COUNT         1100 a         1711 c         1724 g          928 t
ORIGIN      
        1 atagcgcgtg atccgcgctg gggacggatc tcggagggcg ccggcgaaga cgtctacctc
       61 ggcagcgaaa ttgcgcaagc aagggtacgc ggcttccagg gcgaggatct cggcgccgtc
      121 gacacgatcc tcgctactgc aaagcattac gccgcctacg ggcaggcgca ggcgggacgc
      181 gactaccaca ctaccgacat gtcgctgcgc gagctgtggg atacctacct gccgcccttc
      241 cgggcagcga gcgaggcggg cgtggcgacc tacatgacgg cgttcaacga cctgaacggt
      301 gtccccgcga cggcgaacag ttacctgctg acggagatcc tgcgaacgcg ctggagcttc
      361 gacgggttcg tggtctccga ctacacctcg gtcaacgaac tggtgccgca cggcttcgcg
      421 cgcgatgaaa agcacgccgc cgagctcgcg atcaacgcgg gcgttgatat ggacatgcag
      481 ggtgcgagtt tcatgaagca cctcccgcag ctcgtgaagg aagggcgtgt cgatgtacaa
      541 accatagacg cggcggtgca gcgcatcctc gagatgaagt accgtctcgg cttgttcgaa
      601 gatccgtacc gctattcgaa cgaagcgcgg gaagccgcag cgatatacgc cgaagagcat
      661 ctcgaggcgg cgcgtgacat ggcgcggaag tcgatcgtgt tgctgaagaa cgagcgggac
      721 ctgttgccgc tttccgccga cgtctcgctg gcggtcatcg ggcccctcgc cgacagcgaa
      781 ccggacatga tcggttcctg gtccgccgca ggcgaacgcg aggaccggcc ggtgactttg
      841 cttgaaggaa ttcgcgagcg cgtgggtgag caggcagata ttcatttcga ggagggtgca
      901 agttatcgct tcggggatga agacgaacgc gcagggttcg atgccgcact tgctgcggca
      961 ggccgggcgg acgtcatcat cgccgcaatg ggcgaaaggt gggacatgac cggcgaagcc
     1021 gccagccgta cctcgctcgg gctgccgggc aaccagctcg cgctgctcaa ggagctgcgc
     1081 aagctcggca agccggtcgt cctggtgctg atgaacggca ggccgctggc gctcgagtgg
     1141 gccgatgaga acatccacgc gatcctcgag acgtggtacg cgggcacgga aggcgggcac
     1201 gcgatcgcgg acgtcctgtt cggcgactac aatccttccg gaaagttgcc cgtaaccttc
     1261 ccgcgtaacg tcggccaggt gccgatctac tacaacatga agaacaccgg ccgcccgtac
     1321 actgcgggag ccgcggagca gaagtacaag tcgcgttacc tggcgacgcc gaacagtccg
     1381 ctttacccgt tcggctacgg cctcagctat accgggttca cgtattccga tctcgcgctg
     1441 gaccgggaga caatgaggga gggtgagagc atcaccgtga gcgtgcgcgt gacgaattcc
     1501 ggcgaggtgg acggggagga cgtggtgcag ttgtacacgc gagacctggt cggctcggtg
     1561 acgcgcccgg ttaaggaact gaaaggtttc cggaagatct cgctggatgc cggagcaagc
     1621 cgggtggtcg agttcacgct cgatccttcc gacctcgcat tccaccggca ggacatgagc
     1681 tacggcgccg aagccggcaa gttcaaggtt ttcgtcggcg gcaactcgga ggaactcctg
     1741 gaagccggct tccgccttac cgacgacgtc cggttgagcg acgccccgcc gtaggagccc
     1801 gccctgcggg cgacattacg ttgacatttg cgctatcgac ggtcgcccgt gggacgcgct
     1861 cctacagcgc cctgttccag ccgccgcgaa gcgtgatagg tttactcctg tgccttccgc
     1921 gcaaagcggc aagatttcaa tgaaagcacc taaacccaca tcgcgggaca tcgcgttcga
     1981 agccggcgta tcgcaggcga ccgtgtcgcg cgccttgcgc aacagcccgc tcgtgcggcc
     2041 ggaaacgcgg caaagaattc acgacatcgc ggcgcaactc aattaccggg tcgataagca
     2101 cgccgccggg ctgcgcacac ggcaaagccg cacgatcgcc ttgctcctct tcgaggaccc
     2161 gacgtccgac gattcacaga tcaatccttt tttcctctca atgctgggca gcattacgcg
     2221 cgccgccgcc agcatcgatt acgatgtgct cgtatcgttc cagcaattga gcaaccactg
     2281 gcatggcagg tacgaggcga gtcatcgcgc cgacggcatc atcctgctcg gttacggcga
     2341 ctacgtcact tatggcgaga agctgaaagc gctcgcggaa gcgaacgcgc acttcatcat
     2401 ctgggggccg atcatcgacg agcagcccgg ccactacctc ggctgcgaca atatcggcgg
     2461 tgctctgcgg gcaacgcagc acctggttgg tctcggccgc aggcacatcg ccttcctggg
     2521 cggtgcttcc gaacattgcc cggagttcga gcatcggcac cggggctacc tgcaggcgct
     2581 acggaacgcc gccatcgcgc cggaccccga cctgcgcgta gatgccgaca accaggaaag
     2641 ctccgggtat gccgctgcag ctgcgttgct gaagaccggg aagcaggtcg acggcatttt
     2701 cgctgccagc gacctgatcg ctatcggcgc catccgggcg ctacaggacg cggggctgga
     2761 cgtgccgcgg aacgtcagcg tcgtcggctt cgacgacatt cccgccgcgt cctacgtgaa
     2821 cccgggcctg accaccgtgc accaggacac ggcaagagcc ggtgaactgc tggtgacgaa
     2881 cctgatcaag tcgatcgaag gcgagcgcgt ggaatcctcg ctgctgccgc cgacgctggt
     2941 gatacgccag tcctgcggcg cggtggctga gtctaatggc cgccaggctc tgacgagcga
     3001 tccacgaaca gcgtcgccac gcctgctgcg accatgaacg cgccgcccgt gaccagcccg
     3061 aaaatgctcc tgccctcgaa gaactcgcgc agcagcaggc ctaagatgct tgcggcaacg
     3121 agctgcggga tgacgatgaa gaaattgaat attcccatgt acacgcccat cttccgcgca
     3181 ggcaggatgc tggagagcaa cacgtagggc agggacagga tcgacgccca cgcgatgccc
     3241 actccgatca tcggcagcaa cagcatcgac ggctcgcgaa caaagaagaa actcaaaagc
     3301 ccgatgccgc ccagcgagag attgacggca tgacagcggc gacagccaag ccacccggca
     3361 acgaacggga tcatgatcgc cgcaagcgcg gcgaagccgt tgtatgcggc gaacagcacg
     3421 ccgacccagt ccgcgccgtc gttgtagagc tgcgacgtga cgtcgagagt gccgtagtgg
     3481 tgactggtaa cggccgcggt gccgtatatc cacatcgcaa acaggctgaa ccaggaaaaa
     3541 aactgcacga ccgcaagctg ccgcatcacc cgcggcatgt gaaagaggtc gtgcacgatg
     3601 ctgtagaggc cgttggacgt ccggttcctt gactgcatcg ctccgctcag tacctggata
     3661 gcaccgaaga gcgcggcgcc gagcgccagt acgtatagct gcttgtcgag gtctgatccg
     3721 acgatccaga caaggagagc accgccgccg agaatccaga tcagtccacc aatcgtgtac
     3781 tttgccgccg gtctgcctgc gtcacgcttc gatacatcag cctggctgcg cgcggcttcg
     3841 aaagcatcga gttcggcggg ggaatactcg cgcgttctca agacggtcca gagtaccgcc
     3901 gcgagaaaca ctgcgccgcc gaaatagaac gaaagcttta cggactccgg aatgacgcct
     3961 gccggtgccg tgttactgat gtcgaaccag ttggtcatca tccacggcag cgccgatgca
     4021 acgaccgcgc ccaagccgat gaaaaaactc tgcatggcga agccgttcgt gcgctgctcg
     4081 tcgggcagca tgtcgccgac gaaggcgcgg aagggctcca tagagacgtt gatgctggca
     4141 tccatgatcc agagcatgcc tgcggcaacc cacagcgccg gcgaattggg catcacgaag
     4201 agcgcgagcg aggcgaggat ggcgccgacc aggaaatacg gccggcggcg gccaaacctt
     4261 ccccaggtgc ggtcgctcat gtggccgatg agcggctgca ccaaaaggcc tgtgaccggc
     4321 gcggccaccc acaggatcgg caggtcgtcg atggatgcgc cgagcgtctg gaagatgcgg
     4381 ctgacgttcg cgttctgcag ggcgaagcca aactggatgc cgagaaagcc gaaagacatg
     4441 ttccagatct gccagaagcc gagccttggc ttggtccgca acgtgatctg ttcggcaatt
     4501 tcccccacga aattcacacc ttgtattggt agaagcgctg tgggagcccg ccatgcgggc
     4561 gagtacgaaa aacgtcagcg aactcgcccg catggcgggc tcctacaagt taacgggctc
     4621 cttcagtctt taacgcgtcg ccctgaaacg tatcgctgcg ccgccgcctg ctgcgagccg
     4681 caggtcgagc acatcatctc gcgaaaaccc gcgttcttct atgacgagtt catagggagc
     4741 cgtgtaccag tgggcgtcgt cgccgtcacg gtagatttcc gcgacgtact ctgtgctctc
     4801 gtcaaggaac gatagcggca gcgacaggct gcgggcattt tcgtccgtta gcgcgcccag
     4861 gtaccagtct gctccgtcgc gctccttgcg cgcgaacgcg acgaagtctc cgacctcgcc
     4921 ggcgacggca acgctctgct cccagtccgt cggcacatcg acgatgaact ggaaggcgtc
     4981 cggccgctgc tcgtaattct ccggaaggtc ggctgccatc tgtatcgggc tgtacagcac
     5041 cacataaaga gccagctgct tcgccagcgt ggtctttacc cggaaaggcg aattggcgcc
     5101 cttgaacgtc aggtcgaaga ccccgggagt aaaatccatt gggcccgaca gcatccgcgt
     5161 gtacggcagg atggccgtat gctccggcgg attgggcggc tcgccccagg cgttatattc
     5221 ctgcccgcgc gcgccttcgc gtgaaagcca gttcgggtat gtccggcgca aaccggtgtc
     5281 cttgaccggt tcgtgcggat tcaggctgat cctgtgtttc gcagcttccg tcaatgcgcg
     5341 caggtgatgg ttgacgctga actgcccgtc gtgccactcg aagcgctgga tcccgttttc
     5401 gtcgacccgc ttaagctcac cggcatcggc gacgtaaccg gtcttcacct tgcgtactcc
     5461 gag
//
LOCUS       DASUOM010000217         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1094074,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000217 DASUOM010000000
VERSION     DASUOM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094074"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1307)
                     /locus_tag="VFY27_04525"
     CDS             complement(162..1307)
                     /locus_tag="VFY27_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5764809.1"
                     /translation="MSDSSAPETWLAQGLHYVDPVTGAVVPPISPSTTFARDASYRLV
                     SSSHSYARDENATFPLVEQMLARLEGGAAALLFSSGMAAAMAVIQSLEPGDHIVAPKV
                     MYWGLRNWLVRFCDRWGLEISLYDSTLPGDLERAVQSGTTKLVWIETPCNPTWDVVDI
                     AEAAAIAHNIGARLAVDSTVPTPVLTRPLEHGADIVMHSATKYLNGHGDVVAGALVTK
                     REDEQWRHIEEIRAEGGAILGSFEAWLLQRGMRTLFLRVGKASAAALQIARHFENHPQ
                     LERVLYPGLPSHPGHEIARRQMAGGFGGMLSLRVKGGEEAALAVASRLKTFIRATSLG
                     GVESLVEHRYSIEGPNSPIPKNLLRLSIGIESTDDLIADLEQALSGP"
     gene            complement(1362..1736)
                     /locus_tag="VFY27_04530"
     CDS             complement(1362..1736)
                     /locus_tag="VFY27_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764810.1"
                     /translation="MTQKPVGGNNLLPYIAAALAGLVVCMAISVATGRREAWDAGSYY
                     SAGIPIMVVLIFLIAWVFPDRPWRWTLAMAAGQSLSAFLQGSSLNLLPLAVIFMTVIS
                     IPQFVSGYLAARWSRRRDAAKA"
     gene            1983..>2164
                     /locus_tag="VFY27_04535"
     CDS             1983..>2164
                     /locus_tag="VFY27_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5764811.1"
                     /translation="MSFHSLGLSAELLRAVSDQHYDTPTPIQKQTIPVILAGRDVLAV
                     AQTGTGKTAGFALPIL"
BASE COUNT          426 a          678 c          675 g          385 t
ORIGIN      
        1 catcgggcgt cttcccgacg ccgcgacgcg aacccgcatc gaggaatacc ggcaagcggt
       61 gtgaggaaaa ccacggaacc cgcacgcggc acgatcgatg cgaccgcagg agcccgagcg
      121 ggcgagtgcg ctcagcggaa ctcgccaggc aaggcgcacc ctcaagggcc agacagcgcc
      181 tgttcgaggt cggcgatcag gtcgtcggtg gattcgatgc ctatggacag ccgcaacagg
      241 tttttcggga tcgggctgtt tgggccttca atggagtagc ggtgttcgac gagactttcg
      301 acgccgccca gcgaggttgc tcttataaag gtcttcaggc ggctggccac cgccagcgca
      361 gcctcctctc ctcccttgac gcgcagcgac agcatgccac cgaagccccc ggccatctgg
      421 cgccgcgcga tctcgtggcc cggatggctc ggtaaaccgg gatagagcac gcgctcgagt
      481 tgcggatgat tttcgaagtg tcgagctatc tgcaaggccg cagcgctggc tttgccgaca
      541 cgcaggaaca gcgtgcgcat tcctcgctgc agcaaccatg cctcgaacga tccgaggata
      601 gcgccgcctt cagcgcggat ctcctcaata tgtctccact gctcgtcttc gcgcttcgtt
      661 accagggcgc cggcgaccac gtcgccgtgt ccgttcaggt acttcgtcgc ggaatgcatc
      721 acgatgtcgg cgccatgttc cagcggtcgc gtgagcaccg gcgtaggcac cgtggagtcc
      781 acggcaagcc gggcgccgat attatgtgca atcgcggctg cttcggcgat gtcgacgacg
      841 tcccaggtcg ggttgcaggg cgtttcaatc cataccagct tcgtcgtgcc cgattgaacc
      901 gcgcgttcca ggtcgcctgg cagggtcgaa tcgtagaggc tgatctcgag gccccagcgg
      961 tcgcagaatc tgaccagcca gttccgcaag ccccagtaca tgactttcgg ggcgacgatg
     1021 tgatcgccgg gctcgagcga ttggataacc gccatcgcag ccgccatgcc ggaactgaac
     1081 agcagcgcgg ctgcaccgcc ttccaggcgc gcgagcattt gctccaccag cgggaaggtc
     1141 gcgttctcgt cgcgggcgta gctgtgcgat gacgaaacga gccggtaact ggcatcgcgc
     1201 gcgaatgtcg tcgacgggct gatcggcggc acgacggcgc cggtgacagg atcgacgtag
     1261 tgcaggcctt gtgcgagcca ggtctcagga gcggaactgt cgctcattcg cctgctcgca
     1321 tatcggttgc aggcatcggc tggcccggct ggttaaccgt ttcaggcctt cgccgcatca
     1381 cgcctgcgtg accagcgggc ggcaaggtaa ccggagacga actgggggat cgaaatgacc
     1441 gtcatgaaaa tgacggcgag cggaagcagg tttagcgaac tgccctggag gaaagccgag
     1501 agcgattgac cggccgccat ggccagcgtc caacgccacg gacgatccgg aaacacccag
     1561 gcgatgagga aaatcagcac gaccatgatc ggaatgccgg cagaatagta ggagccggca
     1621 tcccacgcct cgcgtcggcc tgtcgcgacg gagatcgcca tgcaaacgac caaaccggcc
     1681 aatgcggcgg caatgtaagg caaaaggttg ttgccaccaa caggcttttg ggtcatgccg
     1741 gcaatgatac tgaaattctc ctgacggcgt aatcgggtgg agaaacgcaa agggtgcggg
     1801 tgcttgcata agtcctgccg tatatataga gtgcgccgca tcctgatgca ctccggtctg
     1861 catcacgcca ttcgcggcgc ccccaggggc cagtctcgag tcaaattttc cttaaacttt
     1921 ttttgcctgg accggccccg gcgcgaaagc gtcgagcggg tgatcctgga gcaatactca
     1981 atatgtcatt ccattctctc ggtctctcgg ccgagctgct tcgcgctgtg tccgatcaac
     2041 actacgacac gccgacgccc atacagaaac agaccatccc cgtgatcctc gcaggccgcg
     2101 acgttctcgc ggtggcgcaa acgggcaccg gcaagacggc cggctttgcc ctgccaatcc
     2161 ttca
//
LOCUS       DASUOM010000218         8546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_632450,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000218 DASUOM010000000
VERSION     DASUOM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632450"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="lptA"
                     /locus_tag="VFY27_04540"
     CDS             complement(<1..631)
                     /gene="lptA"
                     /locus_tag="VFY27_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX5764812.1"
                     /translation="MTRIRRYSSLILAAVSVTWPVALLAQEPDLRLPITVDADSVAYD
                     GRNSMLMYQGLKLNQGSIGIEADEGRASNLDFEDSVWEFTGNVVINTENGRVECDTAH
                     LKFSGHQLERATITGSPATFEMQRPDSDVVTYAEAGRLEYDFNAGVIEFSEQATITEG
                     SNQISSNYLVYNIEEQRINARSAGEGEPRVKIIYTPSDSEASEDDDSASQ"
     gene            complement(646..1203)
                     /gene="lptC"
                     /locus_tag="VFY27_04545"
     CDS             complement(646..1203)
                     /gene="lptC"
                     /locus_tag="VFY27_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5764813.1"
                     /translation="MSARNVLGFLLLVVAAAGSWFLTISLRPPEVEEAARNTRSQGFY
                     LRSARMLGTDTDGDLLYEIEAEYAEQLPNEEIKFRNVRISYRPEATVPWTLNADTAVI
                     GKSQERVVLTGDVVAVSSEGLSGEATEIRTSYLELDPATYRAETDSRVQILVGSRTLT
                     ATGMLALLQEDKLQLKSNVSGRFFP"
     gene            complement(1203..1718)
                     /locus_tag="VFY27_04550"
     CDS             complement(1203..1718)
                     /locus_tag="VFY27_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5764814.1"
                     /translation="MIKRNSSPVPHDVRLAAFDVDGVFTDGRFYLSDAGVETKAFSTQ
                     DGFGIKKLLAADVQVAVISSRRSGAVDTRMRELGVRHVIQGAADKAAAFDLLIESLQL
                     TAGQCVYVGDDVPDLPVLTRVGFAIAVANAVDEVKKCCDYTTRSAGGFGAVREVCDLL
                     LAARTNNRAYG"
     gene            complement(1715..2698)
                     /locus_tag="VFY27_04555"
     CDS             complement(1715..2698)
                     /locus_tag="VFY27_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5764815.1"
                     /translation="MQTKLTPDELLKLAGRVLEIESRAVLQLRQRLDSSFTAACKLCL
                     ETTGRVVVTGMGKSGHIGGKIAATLASTGTPSFFMHPAEASHGDLGMITEQDLLLALS
                     YSGETQEVITILPLVKRMGARLLSLTGKPGSTLARAADVHLNVAVDEEACPLNLAPTA
                     STTATLAMGDALAVALLESRGFTAEDFARSHPSGSLGKRLVLRVSDVMHTGDEVPAVT
                     ERVKLAEGLVEMSRKGLGMTAIVDRENHILGIFTDGDLRRALDADVDVHRTEMSKVMH
                     RNCKTVLPDVLAAEAVHLMEEHKITGLLVADAENRLIGALNVHDLFRAGIM"
     gene            complement(2734..3699)
                     /locus_tag="VFY27_04560"
     CDS             complement(2734..3699)
                     /locus_tag="VFY27_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5764816.1"
                     /translation="MLGNFLEVVGGLLLLVWGANRFVYGAAAMARNLGVAPLLIGLTV
                     VAIATSAPEILISIVAAWRGAPELAIGNAIGSNIVNIGLVLGLVALIRPVRLSSQTLR
                     REMPALLAVTLLGVSLFLDAFLSRIDGVILLSCLVFVMFWLIKLGLRSSVDDTINLEY
                     EADIPRGVSMGRAIGWFVVGLASLLAGAEFMVNGAIEIARFLGMSEVVIGIVLVAFAT
                     SVPELAVSLVAARKGEYGLAIGNIVGSNLFNILAVVGVAALIAPSSLPPSVLSLHLFV
                     MVAFTLVLFTMTYEQEGTGSITRVEGAALLIAYIAYDIYVVVQNV"
     gene            3923..4168
                     /locus_tag="VFY27_04565"
     CDS             3923..4168
                     /locus_tag="VFY27_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX5764817.1"
                     /translation="MRPEDIHALIEAGLPDATVMVSSDDNTHFEAVIVSSAFSGKRPL
                     ARHKLVYDCLGGLVGNEIHALSIRAFTEDEWRQLQAG"
     gene            complement(4249..5343)
                     /locus_tag="VFY27_04570"
     CDS             complement(4249..5343)
                     /locus_tag="VFY27_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5764818.1"
                     /translation="MARLKPVFIFLFQSVVVGLAVAFLVVFLKPQLLPVFNGTTSGAT
                     RPASYADAVDISAPAVANVYTRRLIQSGPDVDAGDRFRLNTSLGSAVVIDPEGYLVTN
                     FHVVADAAEIRVQLADGRIADPDVVGYDAETDLALLKVELGTLNAIPLGRSANVRIGD
                     VVLAIGNPYGLTKTVTQGIVSATGRGLLGLTTFENFIQTDAAINEGNSGGALVNSLGE
                     LVGINTAVLAQDAGTEGISFAIPIDLVRGVVDEIKRHGRVIRGWVGFVDPQELSRAES
                     AALGIERNVGILVRIIPNGPAAVAGLTDGDVILAINDEPIRTAQQARLLVAALEPGDQ
                     IALQVWRERRSFEVTVTAAERPPDAERPLE"
     gene            5802..6392
                     /gene="petA"
                     /locus_tag="VFY27_04575"
     CDS             5802..6392
                     /gene="petA"
                     /locus_tag="VFY27_04575"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5764819.1"
                     /translation="MMTDDVDRNRRHFLTVATAVTGGVGIALASIPFLVSLRPSARAQ
                     ALGAPVEVPLASLEPGEMVRVIWRGRLVFVLRRSQAMLSHLEQTPPDLLRDPNSDVAE
                     QQPDFAVNPYRSIQPEYLVIEGNCTHLGCAPLPEFQVGPAPDWHGGFFCPCHGSRFDL
                     AGRVYKGVPAPTNLRVPPYRFIQDDVIMIGSETGAA"
     gene            6392..7642
                     /locus_tag="VFY27_04580"
     CDS             6392..7642
                     /locus_tag="VFY27_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX5764820.1"
                     /translation="MATVEREAARPQNSFIRWIDDRFPLLETLRYHATEYYASKNFNI
                     WYVFGVLSLVVLVIQLVTGIFLTMNYKPSSAEAFASVEYIMRDVDFGWLIRYMHSTGA
                     SFFFIVVYLHMFRAMLYGSYKKPRELLWIIGMLIFLVLMAEAFAGYLLPWGQMSYWGA
                     QVIISLFGAIPFIGDGLVEYIKGDYSISDITLNRFFALHVVLLPLALIGLVFVHIVAL
                     HHVGSSNPDGIEIKDYKGPDGVPVDGVPFHPYHTSKDLVAIIIFLMIFTGVVFFAPDM
                     GGYFLEHANFEPANTMVTPEHIAPVWYFTPFYAILRAVPNKLWGAILMGAAVFLPLFL
                     PWLDRSRVRSIRYRGWIYRASLGLFVISFFALGWLGLQPAEGLYVALARVFSIIYFAF
                     FLLMPYYTTIDKTRPVPERVVYYV"
     gene            7635..8390
                     /locus_tag="VFY27_04585"
     CDS             7635..8390
                     /locus_tag="VFY27_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX5764821.1"
                     /translation="MSRLFATLAALSLLVAGSQVFAAAGGAELEPAGNDVGNVASLQR
                     GARNFMNYCAGCHSAKYVRYNSIAKDLGLTEDQVVENLMFNAEKTYETIEASMPLDAA
                     ARWFGQPPPDLSLMARARGTDYIFNFLKGFYLDSESPTGVDNLVLGGTSMPHVLWELQ
                     GYQSAVFEEPEPQAAEAGAEAVFSHFESVSSGALSPEDYDAFVRDTVNFLEYISEPVR
                     GTRRVMGVWVLMFLTVFLIIANMLKNQIWKDVS"
BASE COUNT         1657 a         2594 c         2541 g         1754 t
ORIGIN      
        1 cttgcgacgc agaatcgtcg tcttcggagg cttcggaatc gctgggggta tagatgatct
       61 tgacccgcgg ctcgccttcg ccagccgacc gggcatttat gcgctgttcc tcgatgttgt
      121 aaacgaggta gttgctggaa atctggttgc tgccttccgt gatggtggct tgttccgaaa
      181 attcgatgac gccggcgttg aaatcgtatt cgaggcggcc ggcttccgcg tatgtcacga
      241 catcgctgtc ggggcgttgc atttcgaagg tcgccggcga gccggtaatc gtcgctcgct
      301 ccagctgatg tccggagaac ttcagatgcg cggtgtcgca ctcgacgcgg ccgttctctg
      361 tattgatgac aacgttgccg gtgaactccc agacgctgtc ctcgaaatcg aggttgctgg
      421 ccctgccttc gtccgcttcg ataccaatgc tgccctggtt gagcttcagc ccctggtaca
      481 tgagcatcga attcctgccg tcgtacgcga ccgagtcggc gtcgaccgtg atcggcagtc
      541 gcaggtcagg ctcctgggcg aggagggcaa ccggccacgt taccgacacg gcagccagga
      601 tcagtgacga atacctgcgt atcctggtca cgtcacgctc cgacactagg gaaaaaatct
      661 accgctgaca ttggacttaa gctgcaactt gtcctcctgc aacaatgcta gcatgccggt
      721 cgcggtcagg gttcgtgacc ctacaaggat ctgcacccgg ctgtcggttt ccgcacggta
      781 ggttgccggg tcaagctcga ggtaggacgt gcgaatttca gttgcctctc cggagagtcc
      841 ttcgctgctg acggcgacaa catcccccgt aagaaccacg cgctcctggc ttttgccgat
      901 cacggccgtg tcggcgttca acgtccatgg aactgttgct tccggcctgt agcttatgcg
      961 cacgttgcgg aacttgattt cctcgtttgg cagctgttcc gcgtactccg cctcgatctc
     1021 gtacaggagg tccccgtccg tatcggttcc caacatcctg gcggaacgaa ggtaaaaccc
     1081 ctggctacgc gtattgcgcg ccgcttcctc gacctctggc ggccgcaacg agatggtaag
     1141 gaaccagctg cctgctgccg ccaccacgag cagcaggaac ccgagcacgt tgcgcgcgct
     1201 catcagccat acgctctgtt gtttgttcga gccgccagga gcaggtcaca cacctcccgg
     1261 actgctccga agccgccggc agagcgcgtg gtgtagtcgc agcacttctt aacttcgtcg
     1321 acggcattgg cgacagcgat ggcgaaaccg acgcgcgtca gcacaggcag gtccggcaca
     1381 tcgtctccga cgtagacgca ctgcccggcc gtcagttgca atgactctat cagcaggtcg
     1441 aatgccgccg ccttgtcggc ggcaccctgt atgacgtggc ggacacccag ctcgcgcatt
     1501 ctcgtgtcca cggcgcccga cctgcggctg ctgataacgg ccacctgcac atcggccgca
     1561 agaagtttct tgatgccaaa gccgtcctgg gtcgagaacg ctttcgtttc cacaccggca
     1621 tcggaaagat agaaacgacc gtcagtgaag acaccgtcca catcgaatgc ggcgagcctt
     1681 acgtcatgag gtacgggcga tgagtttctc ttgatcacat tatccctgcg cgaaacaggt
     1741 cgtgaacgtt cagcgcgccg atcagccggt tctcggcatc ggccacgagc agaccggtga
     1801 ttttgtgttc ctccatgagg tgtaccgctt cagccgcgag tacgtcgggc aggacggtct
     1861 tgcagttccg gtgcatcacc ttgctcatct cggtcctgtg aacgtcgacg tccgcatcga
     1921 gggcccgacg caggtcgccg tccgtgaaga tgccgaggat gtggttttca cgatcgacta
     1981 tcgccgtcat gccgagcccc ttgcgcgaca tctcgaccag gccttccgcg agctttacac
     2041 gttcagtgac ggctggtacc tcatcgccgg tgtgcatcac atcggaaacg cgaagtacca
     2101 ggcgtttccc gaggctgccg ctgggatgcg aacgagcgaa gtcttccgcc gtgaatccgc
     2161 ggctctccag caacgcaact gcaagggcgt cacccatcgc aagcgtggcg gtggtactcg
     2221 cggtcggtgc caggttgagt gggcacgctt cttcgtcgac ggcaacattc aggtgcacgt
     2281 cggccgccct ggcgagtgtc gagccgggct ttccggtgag ggacaggagc ctggcgccca
     2341 tgcgcttcac cagcggcagg atagtgatca cctcctgtgt ttcgccggaa taagacagcg
     2401 ccaggagcag gtcctgttcg gtgatcatcc ccaggtcccc gtgactcgcc tcggcaggat
     2461 gcatgaagaa cgagggcgta ccggtgctcg ccagcgtcgc tgcaatcttg ccaccgatat
     2521 ggccggactt tcccatgcct gttaccacga ccctgccggt cgtctcgagg cacagtttgc
     2581 acgccgccgt aaatgaactg tccagccgct gcctgagctg caggaccgcc cgcgattcga
     2641 tttcgagaac ccttcctgca agtttcagca gttcgtcggg tgtcagcttt gtctgcaagg
     2701 aagcccccgg cctcgtctgg atagaccggc agtttatacg ttttgcacga caacgtagat
     2761 gtcgtaggca atgtatgcga tcagcagcgc ggcaccctca acccgggtga tgctgcctgt
     2821 gccttcctgt tcgtatgtca tcgtaaacag gaccagcgtg aacgccacca tcacgaacag
     2881 gtgcagggac agtaccgacg ggggtagtga cgaaggtgcg ataagggccg ccacgccaac
     2941 gaccgccagt atgttgaaca ggttagagcc gacgatattg ccgattgcga ggccgtactc
     3001 gcccttgcgc gcggcgacga gagagactgc cagttccggt acgctggtgg cgaacgccac
     3061 cagcacaatg ccgatcacca cctcgctcat cccgagaaag cgcgcgattt cgatagcgcc
     3121 gttaaccata aactcggcac ccgcgagcag cgatgcgagt ccgacgacga accatccaat
     3181 cgcacggccc atgctgacgc ccctcgggat gtcggcctca tactcgagat tgatcgtgtc
     3241 gtccaccgac gagcgcaggc cgagcttgat cagccagaac attacgaaca cgaggcaact
     3301 cagcagtatc actccgtcga tccggctcaa aaaggcatcc aggaacagtg aaacccccag
     3361 cagtgtgacc gcaagcagcg ccggcatttc gcgcctcaat gtctgcgacg acagccgtac
     3421 cggacgaatc aacgccacca ggcccagtac caagccgatg ttgacgatat tcgaaccgat
     3481 ggcgttgcct atggcgagtt ccggagcacc gcgccacgcg gcgacgatgg atatcagtat
     3541 ttccggagcg gatgtggcga tggcgacgac cgtcaggccg atcaaaaggg gcgcgactcc
     3601 gaggttgcgc gccatggcgg cggcgccgta cacaaaccgg tttgcacccc acacgaggag
     3661 aagcaacccc ccgacgacct caaggaaatt acccagcatg ttcgtgattg atgtcggaac
     3721 ctctagccag ggcctttcat gatcgccggg acgaataagc agcgacaaga atgaggcagg
     3781 agcgtgagcg cgatcatgag tggtccgtct ggtagtggtt ctgctcaagg cggctttgag
     3841 cggcacgcca tgataccttc aggcagccgc gctttgttac actgcggacc gccatacctg
     3901 caacttgcca tggataagcc aaatgcgccc ggaagatatc cacgctctca tcgaggccgg
     3961 cctgccggat gcaaccgtca tggtatcgag cgatgacaat acgcacttcg aggcggtgat
     4021 cgtctccagc gcgttctccg ggaagcgtcc gcttgcacgg cacaagctcg tgtatgactg
     4081 cctgggcggc ctggtaggca acgagatcca cgcactctcc atccgcgctt tcaccgaaga
     4141 cgagtggcgg cagctgcagg caggctagat aaagagctac aggagtccgc catgcgggca
     4201 accaaaacgc tgcggcgtgc tgcaccgaca ccgagtttcc cggcgaggct actccagcgg
     4261 ccgctccgcg tcgggcggac gttcggcggc ggttaccgtc acttcgaatg agcgcctctc
     4321 acgccagacc tggagcgcga tctggtcgcc gggttccagt gccgcgacca ggagcctcgc
     4381 ctgctgggcc gtccggatcg gctcatcatt gatggcgagg atgacatcgc cgtccgtaag
     4441 gccggcgacg gcagcgggac cgttcgggat tatccttacc aggatgccga cgtttcgttc
     4501 gatgccgagc gcagcggatt ccgcccgcga aagttcctgc ggatcgacaa agcccaccca
     4561 gccgcgaatg accctgccgt ggcgcttgat ctcgtcgacc accccgcgaa cgaggtctat
     4621 gggaatggcg aagcttatgc cctcggtgcc ggcgtcctgc gcgagcaccg cggtattgat
     4681 ccccaccagt tcgcccagtg aattgaccag cgccccaccg gaattgcctt cgtttatcgc
     4741 ggcatccgtc tggatgaagt tctcgaaggt cgtgaggccg agaaggccgc gaccggtagc
     4801 gctcacgatg ccctgggtga ccgtcttcgt caggccgtat ggatttccaa tggccagcac
     4861 cacgtcgccg atacggacgt tggcggagcg tcccagcgga atggcattca gtgtgcccag
     4921 ttccaccttg agcaatgcga ggtcggtttc ggcgtcgtag cccacgacgt ccggatcagc
     4981 aatgcgcccg tcggcaagct gcacgcggat ttcggcggca tcggcgacga cgtggaagtt
     5041 cgtgacgagg tatccttcag ggtcgatgac caccgctgaa ccgagactgg tattcaggcg
     5101 aaagcggtcg ccggcatcta catcgggacc ggactggatc agccggcgtg tatagacgtt
     5161 tgcgacggcc ggtgcgctga tgtccaccgc atcggcatag cttgccgggc gcgtggcacc
     5221 ggatgtcgta ccattgaaga ccggaagcag ctgcggtttg agaaacacga cgagaaacgc
     5281 gaccgctagc ccgacgacga cggattgaaa cagaaaaatg aaaaccggct ttagtcgagc
     5341 cacgtctctt cctgcagtgc acgaaattag cccgggctat tcatgaatcg ccgcgagaat
     5401 gtttcattat ttgcgaccgg atgcactaga ggcagccggc tcgcagtcgc gaacgacttg
     5461 cggagtggct cctagccaat tttcttgagc cccgtgtata atgccgcatg gttccgcacc
     5521 cagccccgaa ccagctgcca tcctgacggc aaaccgggtc tgctgcaaat ctaccagttt
     5581 tcgaataaag caaaaaatca ccggacttca gaggctagtg tgctgtcccc taaataactg
     5641 cacaattccg gtcgcggcct ttcgcgcctg gcaagacccg atgacgagtt atagcaacgc
     5701 tatttcgagg agtcgcaaca tcgccagggg tcacaagacg caacggaatt ggacagttat
     5761 tgcggtacgg cgcaccagat ccggccggtg catgggagtg cctgatgacc gacgacgttg
     5821 atcgcaacag acgtcatttc ctaactgttg ccaccgccgt aaccggggga gtgggcatcg
     5881 ccctggcctc gatcccgttc cttgtttccc tgaggccaag tgcccgcgcc caggctctcg
     5941 gcgctcccgt ggaggtgccg ctggcatcac tggagcccgg ggagatggta cgggtgatct
     6001 ggcgcggcag gctggtgttc gtgttgcgcc gctcccaggc gatgctttcg cacctggagc
     6061 agacgccgcc cgacctgctg cgcgacccga attccgacgt tgccgagcag cagcccgact
     6121 tcgccgtgaa tccataccgc tccatccagc ctgaatacct ggtgatcgaa gggaactgca
     6181 cgcatctcgg gtgtgcacca ctgcccgagt tccaggtagg gcccgcgccc gactggcacg
     6241 gcggcttttt ctgtccctgc cacggctcgc gcttcgatct cgcgggacgg gtgtataaag
     6301 gtgtaccggc acccactaat ctgcgggtgc cgccgtaccg gttcatccag gatgacgtga
     6361 ttatgattgg cagtgaaacg ggagctgcgt aatggccacc gtcgaacgtg aagccgcaag
     6421 gccgcaaaac agcttcatcc ggtggattga cgaccgcttt ccgctgctgg aaacgcttcg
     6481 ataccacgcg acggagtatt acgcgtccaa gaatttcaat atctggtacg tgttcggcgt
     6541 gttgtcactg gtagtgctgg tgatccagct ggtgaccggc attttcctga cgatgaacta
     6601 caagccgtct tctgccgagg cgttcgcgtc ggtggaatac atcatgcggg atgtcgactt
     6661 cggctggctg atccgctaca tgcattcgac gggtgcatcc ttctttttca ttgtcgtcta
     6721 cctgcacatg ttccgcgcga tgctttacgg ctcctataag aagccgcgag agctgctctg
     6781 gatcatcggc atgctgatat tcctagtgct gatggcggag gccttcgccg ggtacctcct
     6841 gccatggggc cagatgtcct actggggtgc gcaggtcatc atttcacttt tcggcgcaat
     6901 accttttatt ggcgacggcc tggtcgaata tatcaagggg gattattcga tctccgatat
     6961 aacactgaac cgcttcttcg cgctgcatgt cgtcctgttg ccgctggccc tgatcggtct
     7021 cgttttcgta cacatcgtcg cgttgcacca cgtggggtcg agcaatcccg acgggataga
     7081 gatcaaggat tacaaaggac ctgacggagt cccggtggat ggcgtgccgt ttcacccgta
     7141 tcacaccagc aaggacctcg tagcgatcat aatcttcctg atgattttca cgggcgtggt
     7201 atttttcgcg ccggacatgg gcgggtactt cctcgaacac gccaacttcg aacccgcgaa
     7261 taccatggtg acgccggaac acatcgcgcc ggtctggtac ttcacaccct tctacgcgat
     7321 cctgcgtgcg gtaccgaaca agttgtgggg agcgatattg atgggcgcgg ccgtattcct
     7381 gccgttgttc ctgccctggc tcgatcgttc cagggtccgg tcgatccgct atcgcggctg
     7441 gatctatcgc gcctcgctcg ggctgttcgt gatcagtttc tttgcacttg gatggctcgg
     7501 gctgcagcct gcagaagggt tgtatgtcgc actcgcgcga gtcttctcaa tcatctactt
     7561 cgcgtttttc ctgttgatgc cttactacac taccatcgac aagaccaggc cggtgccgga
     7621 gcgggtggtc tattatgtct agattgttcg cgacgctggc tgcactttcg ctgctcgtag
     7681 cagggtccca ggttttcgcc gcggccggcg gtgcggagct ggagccggcg ggaaacgatg
     7741 tcggcaatgt cgcttccctg cagcggggcg cgcgcaactt catgaactat tgcgccggct
     7801 gccattccgc gaagtatgtg cgttacaaca gcatagccaa ggacctcgga ctgaccgagg
     7861 accaggtcgt cgagaacctg atgttcaacg cggagaagac ttacgaaacg atcgaggcgt
     7921 cgatgccgct ggatgcggct gccaggtggt tcggacagcc gccgccggac ctgtcgctca
     7981 tggcgcgcgc gcgcggcacc gattacatat tcaacttcct gaaaggtttc tacctcgaca
     8041 gtgagagccc tacgggcgtc gacaaccttg ttcttggcgg caccagcatg ccgcacgtgc
     8101 tgtgggaact gcagggatac cagagcgctg ttttcgagga gccggagccg caggccgccg
     8161 aggctggcgc cgaggcagtt ttcagtcact tcgagagcgt gtccagcggg gcattgtctc
     8221 ccgaggacta cgatgcgttc gtgcgcgata ccgtcaattt cctggaatac atcagcgagc
     8281 cggtccgcgg tacgcggcgc gtgatgggcg tatgggtgct gatgttcctg acggtattcc
     8341 tgatcattgc caacatgctg aagaaccaga tctggaaaga cgtctcatga ccgccccagc
     8401 ccggactatt catgaatcgc cacgatcctt cccaagactt gttcgcaaac gacattttcc
     8461 gccttggggg aataccagtt ggcatttccg agggagggaa atttcctgtg cggacaagga
     8521 tcaagcaagg gcgcgagcgt attcat
//
LOCUS       DASUOM010000219         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1582578,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000219 DASUOM010000000
VERSION     DASUOM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582578"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFY27_04590"
     CDS             complement(<1..995)
                     /locus_tag="VFY27_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764822.1"
                     /translation="MNAPAFRFRFLLLAGLLPVFDAASAQTVEWWDPAAVAETRFSAF
                     PFPGITDVVATSRGIYSVGMTQCVNDPAFFNVPKCKGLLVKHDAEGDRLWRKLLQDRD
                     DTWITAVVADDSGVYVHGTYRWYQQFPTYESFVHKYAHDGTLLWKFRDSEGNPDGYRI
                     ARIRSQNLLLFDGFLYASSWRGRGSFLRKFTLDGELVWLAPAGAVGTLTAGDGYIFGS
                     AGTNEGTQFETQFQIWDTDGNLVDSFFTETIAENGLAWHDGALYFCAREGRYDRDYYS
                     LNKMQPDGTPVWRIPYGRTAYGTQGCGVHVDDGGVTVAVNLDLRRRATYPANLTVR"
     gene            complement(1334..3730)
                     /locus_tag="VFY27_04595"
     CDS             complement(1334..3730)
                     /locus_tag="VFY27_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764823.1"
                     /translation="MLRSFHQAVFGTQKALLLLLAATPAVADVGAISVEVVDAVTRRP
                     VDGVTITAESRDGDVQSATTDKGRALLDSLEDGFFEMRAESAGYVTAVEPTVRVLEQR
                     TGSLRFELQPVSDSIDEIVVVARAREADPFGAVSNRFLSRDELRNAPGSGSDVMRALS
                     GLPGVVSNGEFAAFSVRGHGPKSNLIYVDGFPFQQVVHFEQTLGEEEEIINGGRYSIF
                     APNAIAGAEFSPGGWSAEFSGRDASLLQLEVVDGAPSPVGSLRLDLAGVELLYEGPSG
                     FHHNTSMFLQARRFDFGQLFEIIEEESLGAPVSTDVLLKTHTRLDDDDEFEFLAIYAP
                     EEYTRDINHILAAEEDEGIEDVTLQNEDQDLALIGGTWRRMIGDDGEWTNRVYYRESD
                     RVSSEGEAFPDLVPPGTPADQVPVREKLLTVQEKQSELGWRSDASFGNRLGILRAGLQ
                     LTSTDLDYSTELREDWIRYLYDSDDPRPPGANYIVLEPGEINSIYSASETNYAAYGEQ
                     VFDWGDANLRAGIRYDRNGFGDEDLVSPRFGFNYSISPRLSLSATAGIFYQSPSNLAR
                     AADPDNFDLESEELTHFSLGFDYWLSENLSLLVEAYYQDLDNRLVESDRTSGRISNDG
                     EGTNTGVDLVLTRNFDQGWSADFVYSWNRYQVDDNDGRGEYDWDFNREHFVAVGGRWE
                     INERWQVAARWKYGSGQPTYRYIVHDDVLAPNPPVRYSQERFDLNADRGDAFHSLDVR
                     VDYRRPIGPVDLVLFADVLNVYGGPAGLQPEFNILTGEIVDEEEETLPLLGLIVEYAW
                     "
     gene            4263..>4553
                     /locus_tag="VFY27_04600"
     CDS             4263..>4553
                     /locus_tag="VFY27_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5764824.1"
                     /translation="MRVLVVEDNRDICENISDYLGARGHITDFAHDGISAMHLALTEP
                     IDVIVLDLMLPGMDGLTFCRKLRLEAKRNTPVLMLTARDTLPDKLAGFDAGAD"
BASE COUNT          931 a         1385 c         1357 g          880 t
ORIGIN      
        1 cggaccgtga gattcgcagg atacgtggcc ctcctcctca ggtcgagatt aacagctacg
       61 gtcacgcctc cgtcatcgac gtgtacgccg caaccttgcg tcccgtaggc cgtccggccg
      121 taagggatac gccacaccgg cgttccgtcg ggctgcatct tgttgagcga gtagtagtcg
      181 cggtcgtaac ggccttcgcg cgcacagaag tagagcgccc cgtcatgcca ggcgaggccg
      241 ttctcggcaa tggtttcggt aaaaaacgag tctaccaggt tgccgtctgt atcccagatc
      301 tggaactgtg tctcgaactg cgtgccctcg ttcgtccctg ccgagccgaa gatgtagccg
      361 tcgccagccg taagggtgcc caccgcgccc gcgggtgcga gccaaacgag ctccccgtcg
      421 agagtgaact tcctcaggaa gctcccccga ccccgccacg aagacgcgta caggaacccg
      481 tcgaacaaca gcaggttttg cgaacggatc cgggcgatcc ggtacccatc cggattgccc
      541 tcggagtcgc ggaacttcca caggagcgtg ccgtcgtgcg cgtacttgtg tacgaacgat
      601 tcgtaggtcg ggaactgctg gtaccaccgg tacgtaccgt gtacatagac gccggagtcg
      661 tcggccacta ccgccgtgat ccaggtatcg tcgcgatcct gcaggagctt ccgccacagc
      721 cgatcgccct cggcgtcgtg cttcacaagc agacccttgc acttgggtac attgaagaac
      781 gccggatcgt tgacgcattg cgtcattccc accgaatata tgccccgaga cgtcgcaacc
      841 acatcagtga tccccggaaa ggggaatgcg gaaaagcggg tttcggcgac ggcggcggga
      901 tcccaccact cgacggtctg cgcggatgcc gcgtcgaaaa ccggcagcaa gccggcaagg
      961 agaaggaaac ggaaacgaaa ggccggagca ttcattggcg tgcacctccg ccagtggtcc
     1021 tggctggtca atgtgctttt ttgccgctcc cggcgtgtag tatacgcgag catgcgactg
     1081 ttttggaatc gagaaggccg aggcgaaggg tcaggatgca cccgtcccgc agccctgttg
     1141 atagctgctg ggacgggtgc agggacgtgt ggcagcgggg gcgtggttgg cgctgttcct
     1201 gcaatgccgg tgcgcgtcaa aagatcacag ccgatatcct aaatgctcca agcgtcatgg
     1261 gtgtgagcaa gcttgaagca ttgtcgttac attggcgcgc tccttttcga gcgtgggctg
     1321 gctactacgc gttttaccag gcgtactcga cgatcaggcc gagcagcggc agcgtttcct
     1381 cctcctcgtc gacaatttcc cccgtcagga tgttgaactc gggctgcagg ccggccggac
     1441 cgccgtaaac gttcagcacg tccgcgaaca gcacaagatc caccgggccg atcggacgac
     1501 ggtaatcgac gcgcacatcg agtgaatgga acgcatcgcc gcgatctgca ttcaaatcga
     1561 acctctcttg cgaatagcga acgggcgggt ttggcgccag cacgtcatcg tgcacgatgt
     1621 agcggtacgt tggttgcccg gagccatact tccagcgtgc cgctacctgc catcgctcat
     1681 tgatctccca gcgcccgccg acagcgacga agtgctcgcg gttgaagtcc cagtcgtact
     1741 cgccgcgccc atcattatcg tccacctggt agcgattcca ggaatagacg aaatcggccg
     1801 accagccctg atcgaaattg cgcgtcaaca ccaggtccac gccggtgttg gtgccctcac
     1861 catcatttga gattcgaccg ctcgtgcggt cgctctccac gagccggtta tcgagatcct
     1921 gataatacgc ttctacgagc agactcaggt tctcgctcag ccaatagtcg aagcccaggc
     1981 tgaaatgcgt aagctcttcg ctctcgagat caaagttatc agggtcggcc gcacgcgcga
     2041 gattacttgg tgactgatag aagatgccag ccgttgccga caagctcagc cggggtgaaa
     2101 tcgagtaatt gaagccgaat cgcggcgaca caaggtcctc gtcaccgaac ccgttgcgat
     2161 cgtagcgaat gcccgcacgc agattggcat cgccccagtc gaacacctgc tcgccgtaag
     2221 cagcgtagtt ggtttcgctt gcgctataaa tcgagttgat ctcaccaggt tccagcacaa
     2281 tgtagttcgc gcccgggggc cgcggatcgt cggaatcgta gagataacgt atccagtcct
     2341 cccgaagctc agtcgaataa tcgagatcgg tgctcgtgag ttgcagcccg gctctcaata
     2401 tcccaaggcg gttgccgaac gacgcatcgc tgcgccagcc gagttccgac tgcttttcct
     2461 gcacggtcag gagtttctcg cgcaccggaa cctggtcggc cggcgtaccc ggcggcacca
     2521 ggtccgggaa ggcctcgccc tcggacgaca cgcggtcgct ctcacggtag tagacacggt
     2581 ttgtccattc gccatcgtcg ccaatcatcc gccgccaggt gccaccgatg agcgcgaggt
     2641 cctgatcttc gttctgcaac gtgacgtcct cgatgccctc gtcctcctcg gcggcgagga
     2701 tgtggttaat gtcccgcgtg tactcctcag gtgcgtatat ggcgagaaac tcgaactcat
     2761 cgtcatcatc gagacgtgta tgcgtcttca ggagcacgtc cgtagaaacg ggcgcaccca
     2821 aagattcttc ctcaataatc tcgaagagct ggccgaagtc gaagcgccgc gcctgcagga
     2881 acatcgacgt gttgtggtgg aagccgctcg gtccctcgta aagcaattcg acgccggcga
     2941 gatcgagccg caggctcccg acaggcgacg gcgcgccatc cacgacctcg agctgcagga
     3001 gcgaggcgtc acggccgctg aactctgcgc tccagccgcc gggcgagaac tccgccccgg
     3061 caatggcatt gggtgcaaag atggagtagc ggccgccatt gatgatttct tcctcctcgc
     3121 ccagcgtctg ctcgaaatgc acgacttgct ggaacgggaa tccatcgaca tagatgaggt
     3181 tgctcttggg gccatggccg cgcaccgaaa aggccgcaaa ctcgccgttg gagacgaccc
     3241 ctggcaggcc gctcagcgcg cgcatgacgt cactgccgct gccaggcgcg ttacgcaact
     3301 cgtcgcggct caggaatctg ttcgagacgg cgccgaaggg gtctgcctcg cgtgcacgag
     3361 cgacaacgac gatctcgtct atcgagtcgg acaccggctg cagttcgaag cgaagactgc
     3421 ctgtccgttg ctccagtact cggaccgtgg gctcgacggc cgtgacgtag cccgccgatt
     3481 ctgcacgcat ttcgaagaaa ccgtcttcca ggctgtcgag cagcgcccga cctttatctg
     3541 tcgttgccga ctgcacatcg ccgtcacgcg actcggccgt aatcgtcaca ccatcgacgg
     3601 gccggcgcgt cacggcatcg acgacctcta cagatatcgc gccaacgtcg gcaacagcgg
     3661 gggttgccgc aagtagcagc aaaagggcct tttgcgtccc gaaaacagcc tgatggaaac
     3721 tacgtaacat ggcgagacct ggtgagaagt ggtgatttcg aggttgatct tgtttgcgcc
     3781 ttggcttgtg ccaggcagcc ccatgggctg aagtattcga gccgctgtta caagtctacg
     3841 cgggcgattc ctggcaggtt gtaaaggagt gtaacaaagt ttgtacctga cggaccggca
     3901 gtgaccgcga gctgaaatgc cgccgggcgc caccgacagc acacaccatc atccgatata
     3961 ctgccgtttt cacttccgct gaaccgccgg aaaaacctgg gatgttctaa cggtagcgta
     4021 gtgactgtga aagagatgtc gccgaaccgt gaccttgccg tcgataagcg aagctggcgg
     4081 ccgattgaaa agcgaaacgc gtcgtcgcag tactgtgaga acgagatgga ggggatgcgg
     4141 tcgttcacgt tttggtcacg ttgcggcgac cgattggtca gccttcgcga cgcaaactag
     4201 aacgtgtcga caacgtccac gggaggacgg caacgatgac acaggctctg cgtgcaaact
     4261 gcatccgtgt actggtggtc gaagacaatc gagacatctg cgagaacatc agtgactatc
     4321 tcggtgcccg tggccatatc acggatttcg cacacgacgg tatcagcgca atgcatctgg
     4381 cgctgacgga accaatcgac gtgatcgttc tggacctcat gctccccggc atggatggct
     4441 tgactttttg ccggaaattg cgcctcgagg cgaaacggaa tacgccggtc ctgatgctga
     4501 cggcgcgcga tacgttgccg gacaaactgg cggggtttga cgccggcgcc gac
//
LOCUS       DASUOM010000220         6364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1058648,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000220 DASUOM010000000
VERSION     DASUOM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058648"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1137)
                     /locus_tag="VFY27_04605"
     CDS             complement(112..1137)
                     /locus_tag="VFY27_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX5764825.1"
                     /translation="MKIVEGVTPEKFRDEIRPASVPAVLKGLAADWPAVREGLQSTRA
                     IADYLRRFDQGRPVETIFGSPEIEGKFFYNEKLDGLNFERRPEAIAASVDRILAAAEQ
                     ERPPSIYVQSVPIRDCLPAFSQENRLGIVDPSVSPRIWIGNRLTVQTHFDLSENVACV
                     VAGRRRFTLFPPEQTPNLYPGPFELTLAGPPVSMVRLEDPDLGRYPRFGEALEHGLVA
                     DLEPGDAIYIPYFWWHHVQTLDDLNVLVNYWWNDAQADLGSPFDALLHAILAIRDMPP
                     SQRTAWKVMFEHYAFGENGDPVEHLPPQARGALGAHDPRMRQQIRMMLLNAIARQAGI
                     RPPPRQG"
     gene            complement(1309..2652)
                     /gene="xylA"
                     /locus_tag="VFY27_04610"
     CDS             complement(1309..2652)
                     /gene="xylA"
                     /locus_tag="VFY27_04610"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX5764826.1"
                     /translation="MSTAPFIASREFFPGIDRIQYEGPESDNPLAFKAYDADRVVGGK
                     TMRDHLRFSICYWHTFCAEGGDPFGPGTRSYPWKEKRDPMAAAEDKLDAAFEFFSKMG
                     VPFFCFHDRDIAPEGSSVLESERNLQRMVELARQRQAATGIRLLWGTANVFSHPRYMN
                     GASTNPDFDVVAHAGAQVKAALDATVALGGQNYVFWGGREGYSCLFNTSTGRELDHLA
                     RFLSMARDYGRSIGFEGTFLIEPKPMEPMYHQYDADAQTVIGFLRHYGLAGDFKLNVE
                     ANHATLAGHSFAHELQMSADAGLLGSIDANRGNPQNGWDTDQFPTDLYDAVQAMLVLL
                     GDGGFKTGGFNFDAKVRRESTDPEDMFIAHIGGMDTFARGLLIAHDILENSPLPEFRR
                     QRYASFDDGPGSAFESGKLSLTDLRDHAAKSGEPAQFSGKQEWVENLINDYIFRS"
     gene            complement(2649..4160)
                     /gene="xylB"
                     /locus_tag="VFY27_04615"
     CDS             complement(2649..4160)
                     /gene="xylB"
                     /locus_tag="VFY27_04615"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX5764827.1"
                     /translation="MNTVLGIDLGTQSLKVIFYDYEARQFVASAAAPLEVSRDNHGRA
                     EQEAGWWLVALAKALGEVRPDVRASVRAIGVSGQQHGFVPVDADGRVLAPVKLWCDTA
                     TQKEADEITEAAGGRDNCIEFAGNPVLTGYTAPKIRWLAKHEPDAYRQMSHILLPHDY
                     LNYILTGRMAMEFGDASGTALLDVRDRSWSRHMLDAVDPERDLEPCLPPLLEPAEILG
                     KTSDSFSDEFGLPAGIPVAPGGGDNMMAAVGTGNVSRGKLTMSLGTSGTLFAFSDTPV
                     VDPAGNIAAFCSSTGGWLPLLCTMNCTYATELMKKPLDVTNETFDETIGAIGAGSDGL
                     TALPFFSGERTPNLPRATASFLGLNAGNCSRGHLLRATVEGATFGIRVGLDSLRELGL
                     AGSEIVLTGGGSKSRAWCQIVADVCDLPVTLLAQDEGASFGAALHALWILQRQDDRSI
                     TIDDITRQHITLKPELSRAPDSANVSRYQESYAAYLQAVAQLAPLFETSGVTP"
     gene            complement(4176..>6364)
                     /locus_tag="VFY27_04620"
     CDS             complement(4176..>6364)
                     /locus_tag="VFY27_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5764828.1"
                     /translation="DKAEILDESLELDLEKLGQKYPAGIGQFSRPSDSRGPISPRVEP
                     RRDARETVELVNALQKFALEETRLGIPMFFHEEGLHGYAAVGATSFPQAIGLASTWDR
                     SLVRDVNDITAREIRARGVHLVLSPVVDIARDPRWGRIEETFGEDPFLVSEMGIASVR
                     GLQGELIDGQLAPGKVFATLKHMTGHGQPESGTNVAPAPFSERELRENFFPPFEAAIR
                     DAGARLVMASYNEIDGVPSHVNEWLLRDVLREEWDFDGVVVADYYAIDQLVGLHHVAA
                     DMRQAAVRAFKAGVDMDLPNGNAYPTLLDALRDGEVNEAAIDAAVKRVLELKFRSGLF
                     EEPFADAAYAEQVTNNDEARALARKAAERSIILLKNDGMLPLLLPDAGRSSNRQVIAV
                     VGPNAAVARLGGYYGEPPHTVSILDGIREKVGDRAEVRFAQGVFITENDDWWADEVVL
                     ADPEKNRRLIREAVEVARDADVIVLAIGDTEQTSREGWGDNHLGDRASLDLVGEQQEL
                     FDALRALNIPLAVVLINGRPASTVEISDKANAILEGWYLGEQGGHAMADVLFGEVNPG
                     GKLPVTVPRHVGQLPMYYNYKPSSKRGYLFDTTEPLYPFGWGLSYTTFELGSPRLSES
                     RIGTDGSVNVEVSVRNTGRVAGDETVQLYVRDLVASVTQPVKELKGFERVTLAPGERT
                     TVRFTLTPESFRMWNDRMERVVEPGEFEIMTGPNSVELESVTLTVR"
BASE COUNT         1116 a         2030 c         1994 g         1224 t
ORIGIN      
        1 agtagggaac aatgcacctg taggagcccg ccatgcgggc gagtgcgata agcgtcagcg
       61 aactcgcccg catggcgggc tccaacaggt attgatcggg ctccttcggc gctagccctg
      121 gcggggcggc gggcggattc ccgcctgcct tgcgattgca ttcagcaaca tcatcctgat
      181 ctgttgccgc atgcgcgggt cgtgcgcgcc gagagcgccg cgagcttgcg gtggcaggtg
      241 ttcgaccgga tctccgttct ccccgaacgc gtagtgctca aacatcacct tccacgcggt
      301 gcgctggctg ggtggcatgt ccctgatcgc aaggattgcg tgcagcagcg cgtcgaatgg
      361 tgagccaagg tcggcctgcg cgtcgttcca ccagtaattg accaggacgt tgaggtcgtc
      421 cagcgtctgc acgtgatgcc accagaaata ggggatgtag atggcatcgc ccggttcgag
      481 atccgcaacc agcccgtgct ccagcgcctc gccaaaccgc ggataccgcc cgagatccgg
      541 atcctcgagc cgcaccatgc tgaccggagg acccgcgagc gtcagctcga acggaccggg
      601 gtagaggttg ggtgtctgct ccggtggaaa tagcgtaaat cgcctgcggc cggcgaccac
      661 gcaggcgacg ttctcgctca ggtcgaaatg cgtttgcacc gtaagccggt tgccgatcca
      721 gatccgcggc gacacggagg gatcaacgat cccgagccgg ttctcctgcg agaacgcagg
      781 caggcagtcg cggatcggca ccgactgtac gtagatggac ggcggccttt cctgttccgc
      841 ggcagccagg atgcgatcga cggaggcggc gatcgcctcc ggccgtctct cgaaattgag
      901 gccgtccagc ttctcgttat aaaaaaattt tccttcgatc tccggactgc cgaagatggt
      961 ctcgaccggc cggccctggt cgaagcggcg gagatagtcg gcaatggcac gcgtcgactg
     1021 caggccctcc cgcacggcgg gccagtccgc agccaatccc ttcagcaccg cgggcacgct
     1081 ggctggccgt atttcgtcgc ggaacttctc cggagtcacg ccttcgacga tcttcattcg
     1141 ggggagggtg tactccaccg ttcacctcgg ttgggccgtg gggaagctct gcataagtcg
     1201 gaaaggagca ctgtaggagc tcgccatgcg ggcgagtgcg tcgagccttg tcgcactcgc
     1261 ccgcatggcg ggctccccca ggtggcttct gcagaggctg ctatagcctc aggagcggaa
     1321 tatgtaatcg ttgatcaggt tctcgaccca ctcctgcttg ccgctgaact gcgccggctc
     1381 gcccgatttc gcggcgtggt cgcgcaggtc ggtgagcgaa agcttgccgc tctcgaacgc
     1441 cgaacccggg ccgtcatcga agctcgcgta gcgctgcctg cggaattcgg gcagcggtga
     1501 gttttcgagg atgtcatgcg caatcagcag gccgcgggcg aaagtgtcca tgccgccgat
     1561 gtgcgcgatg aacatgtctt ccggatccgt ggattcgcgc ctgaccttcg cgtcgaagtt
     1621 gaaccccccg gtcttgaagc cgccgtcgcc gagcaatacc agcattgcct gcacggcatc
     1681 atacaggtcc gtcgggaact ggtccgtgtc ccagccgttc tgcgggttcc cgcggttcgc
     1741 atcgatggat cccagcaatc cggcatccgc gctcatctgc aattcgtgcg cgaacgagtg
     1801 ccctgcgagt gtggcgtgat ttgcctcgac gttcagcttg aagtcgcccg cgaggccata
     1861 gtggcgcagg aagccgatga ccgtctgggc atccgcatcg tactggtggt acatcggctc
     1921 catcggcttc ggctcgatca ggaacgtccc ctcgaagccg atcgagcggc cgtagtcccg
     1981 cgccatcgac aggaaccgcg cgaggtgatc gagttcgcgc ccggtgctgg tattgaacag
     2041 gcacgagtag ccttcgcggc cgccccagaa cacgtagttc tggccgccga gcgcgaccgt
     2101 cgcatcaagc gccgccttga cctgcgcgcc cgcgtgcgcg acgacgtcga aatccggatt
     2161 cgtcgaagcg ccgttcatgt accgcgggtg agaaaatacg ttcgccgttc cccatagcag
     2221 cctgattcct gtcgcggcct gcctctgcct ggcgagctct accatgcgct gcaggttacg
     2281 ctcgctttcc aggaccgaag atccctccgg cgcgatgtca cggtcgtgga agcaaaagaa
     2341 cggcacgccc atcttgctga agaactcgaa tgcagcgtcg agcttgtctt cggcggccgc
     2401 catcgggtcg cgcttttcct tccatgggta tgaacgggtg ccgggaccga acgggtcgcc
     2461 accttcggca cagaatgtgt gccagtagca aatggagaag cgcaggtgat cgcgcatcgt
     2521 cttcccgcca actaccctgt cagcgtcgta ggctttgaac gcgagtggat tatccgactc
     2581 cggcccttcg tactgaatcc ggtcgatccc cggaaagaat tcccggctgg cgatgaatgg
     2641 cgcggtactc atggtgttac tccactggtt tcgaacaagg gagccagttg cgcgaccgcc
     2701 tggagatacg cggcgtaact ttcctggtag cgggaaacgt tcgcgctgtc gggtgcccgg
     2761 gaaagctctg gctttaaagt gatgtgctgt cgggtgatat cgtcgatggt gatcgaccgg
     2821 tcgtcctggc gctgcaggat ccacagggcg tgcagggcgg cgccgaagga cgcgccctcg
     2881 tcctgggcga gcagggttac cggaagatcg cagacgtccg ctacgatctg acaccaggcg
     2941 cggcttttcg atcctccacc tgtcaggacg atctccgacc cggccagacc cagttcccgc
     3001 agcgagtcga gcccaacgcg gatgccaaaa gtcgcgccct ccacggtcgc gcgaagcagg
     3061 tgcccgcgac tgcagttgcc ggcgttcagc ccaaggaagc tcgcggtcgc gcgtggcagg
     3121 ttcggcgtcc gttcgccgct gaaaaagggc aatgccgtca atccgtccga gccggctccg
     3181 atcgcgccga tagtttcgtc gaacgtttcg ttggtgacgt ccagcggctt tttcatgagc
     3241 tctgtcgcgt aagtgcaatt catggtgcaa agcaatggaa gccagcctcc cgtcgaactg
     3301 cagaaggcgg cgatgttgcc ggcagggtcc acgaccgggg tgtcagaaaa ggcgaacagc
     3361 gtgccggagg tgccaaggct catcgtgagc ttgccgcgcg agacgttgcc ggttccgacc
     3421 gcagccatca tgttgtcgcc accacctggc gccaccggga tgcctgcagg caggccgaat
     3481 tcgtcggaga aggaatcgct ggttttcccc aggatttcag ccggctccag caagggagga
     3541 aggcaaggct cgaggtcccg ctccggatcg accgcatcga gcatgtggcg gctccagctt
     3601 cgatcacgca cgtcgagcaa cgcggtgccg gaggcatcgc cgaattccat cgccatcctg
     3661 cctgtcagga tgtaattgag gtaatcgtgg gggagcagga tgtgcgacat ctgccgatag
     3721 gcgtccggct cgtgctttgc cagccagcgg atcttcggcg cggtgtagcc ggtgagcacc
     3781 gggttaccgg caaattcgat gcaattatca cggcctcccg ccgcttcggt gatctcgtcc
     3841 gcttccttct gcgtcgctgt gtcgcaccag agctttaccg gcgcaagcac gcggccatcg
     3901 gcatcgacgg gtacaaaacc gtgttgctgc ccggaaacac cgatcgcccg cacggacgcc
     3961 cgtacatccg gacggacctc gccgagcgct ttcgccagcg caaccagcca ccagccggct
     4021 tcctgttcgg cgcggccgtg gttgtccctc gatacttcaa gaggcgccgc cgctgacgcg
     4081 acgaactgcc gcgcctcata gtcatagaaa atgaccttga gactctgggt gccgagatcg
     4141 atgcctaaga ctgtgttcat ccaggatgcg agggtctacc tgacggtcag ggtgaccgac
     4201 tccagttcga ccgagttggg cccggtcatg atttcgaatt ctcccggctc gacgacccgt
     4261 tccatcctgt cgttccacat gcggaagctt tcgggagtga gcgtgaacct gacggtggtg
     4321 cgttctcccg gagcgagggt cacccgttcg aagcctttca gttccttgac cggctgggta
     4381 acggaggcga ccaggtcgcg tacgtacagc tgtaccgttt catcgccggc aacgcggccc
     4441 gtgttacgca cgcttacctc gacgttaacc gaaccgtccg tgccgatccg gctttcggag
     4501 agccgcggtg atccgagttc aaaagtcgtg taactcaggc cccagccaaa cgggtacagc
     4561 ggctccgtcg tgtcgaacag gtaaccacgc ttggaggaag gcttgtagtt gtagtacatc
     4621 ggcagctgcc cgacgtgtcg cggcaccgtg acggggagct tgcctcctgg attgacctcg
     4681 ccgaaaagaa cgtcggccat tgcatgacct ccctgttctc cgaggtacca gccctcgaga
     4741 atcgcattcg ccttgtcgga gatttcgacc gtcgaagcgg gacgcccgtt gatcaggacg
     4801 actgccagcg ggatgttcag ggcacgcagc gcatcgaaca gttcctgctg ttcgccgaca
     4861 agatcgaggc tggcccgatc gccgagatgg ttatcccccc agccttcgcg gctggtctgc
     4921 tccgtatcgc cgatcgccag gacgatgacg tcggcatcgc gggcaacttc gacggcttcc
     4981 ctgatcaggc ggcggttttt ctcggggtcg gccagcacga cttcatcagc ccaccagtcg
     5041 tcgttttccg tgatgaacac gccctgggca aaacgaacct cggcccggtc accgactttt
     5101 tcccggatgc catcgagaat cgagacggtg tgcggcggct cgccatagta gccaccaagc
     5161 cgcgccactg ccgcattcgg gccaacgacc gcgatgacct gtcggttcga tgagcggccg
     5221 gcatccggca ggagcagcgg cagcatgccg tcgttcttca gcaggatgat gctgcgctct
     5281 gcagccttgc gtgccaaggc gcgtgcctcg tcattgttcg tgacctgctc cgcgtaagcg
     5341 gcgtcggcaa acggctcctc gaacaggccg gagcggaact tgagttcgag tacgcgcttc
     5401 actgcggcat cgatggcggc ttcgtttacc tctccatccc gcagcgcgtc aaggagggtg
     5461 ggataggcgt tgccgttcgg caggtccatg tcgacgcctg ctttgaacgc ccgcaccgcc
     5521 gcctgccgca tgtcggcggc gacgtggtgc aggccgacaa gctggtcgat ggcgtagtaa
     5581 tccgccacca ccacgccatc aaaatcccat tcctcgcgca gcacgtcgcg cagcaaccat
     5641 tcgttcacgt ggctcggcac gccgtcgatc tcgttgtacg acgccatgac caggcgggct
     5701 cccgcatccc tgatcgccgc ttcgaagggc gggaaaaagt tctcgcgcag ctcccgttcc
     5761 gaaaacggag cgggggccac gttcgtgccg ctttccggtt ggccgtgccc ggtcatgtgc
     5821 ttcaacgtgg cgaatacctt ccccggcgcg agttgcccgt caatcagctc gccctgcaat
     5881 ccacggacgg acgcgattcc catctcgctg accaggaacg ggtcttcgcc gaaggtctcc
     5941 tcgatccgcc cccagcgagg gtcgcgagcg atgtccacta ccggggacag gaccaggtgc
     6001 acgccgcgcg cccggatttc gcgagcagtg atgtcgttta cgtccctgac cagggagcga
     6061 tcccacgtgg atgccagccc gatagcctgc gggaagctgg tggcgcccac cgcggcgtat
     6121 ccgtgcagtc cctcctcgtg gaaaaacatg ggtattccca gccgggtttc ttccagcgcg
     6181 aacttttgca gggcgttcac cagttctacg gtttcgcgtg catcccgtcg cggctccacc
     6241 cgcggcgaaa tgggtccgcg cgagtccgat gggcgagaga actggccgat gcccgcgggg
     6301 tacttctgtc ccagtttttc gaggtccagc tcgagggatt catccagtat ttcggccttg
     6361 tcct
//
LOCUS       DASUOM010000221         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1180287,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000221 DASUOM010000000
VERSION     DASUOM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180287"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1477)
                     /gene="hldE"
                     /locus_tag="VFY27_04625"
     CDS             complement(56..1477)
                     /gene="hldE"
                     /locus_tag="VFY27_04625"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HEX5764829.1"
                     /translation="MTLTIPDFSRTRVLVAGDVMLDRYLFGGTSRISPEAPVPVVHVR
                     SSDDRAGGAANVAVNLAALGVTTTLLGVVGRDEEARRLQEVLEARGITCRFSRSDHRP
                     TITKTRVHSRGQQLIRLDREESQGDPAGPSLAASLTAALKDVDAVVLSDYGKGALGDV
                     ASMIEACRLAGVPVMVDPKGTDFSRYRGSSVITPNQSEFEAVAGSAASDDDMVARARK
                     MLRDLELEALLVTRSEKGMLMVKAGEEPFFLSTQAREVYDVTGAGDTVIATFAAAYAS
                     GSDFRSAVGLANLAAGLVVRKIGVASVTPSELSIALHQHGQGGPGMVSEEELLSLLAE
                     CRERGEKIVMTNGCFDVLHAGHVAYLEEATNLGDRLVVAVNDDDSVRRLKGEGRPFNI
                     LADRMAVLAGLASVEWVVPFAEDTPARLIGSVLPDVLVKGGDYRPEQIAGAADVIRNG
                     GEVRVLSFRDGHSSSRIIDKLRS"
     gene            complement(1615..2331)
                     /locus_tag="VFY27_04630"
     CDS             complement(1615..2331)
                     /locus_tag="VFY27_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HEX5764830.1"
                     /translation="MSKTKIDRKIVGYAVKQAPVKDAAADSAEPRRETPEAGRAEVIR
                     MHEKLERPEMLIGSTYKVKTPVSDHAMYVTINDIVLNEGTEHEKRRPFEIFINSKNLD
                     HYQWIVALTRIISAVFRKGGDVTFLVDELKAVFDPRGGYWKAGGKFMPSIIAELGYIV
                     EKHLIMIGLLAKPKLDEEQKKLIEQKRAEFEAAAKQQDAFSKSEYPEGAQLCAKCSTA
                     AVIMMDGCMTCLSCGESKCG"
     gene            2376..>2558
                     /locus_tag="VFY27_04635"
     CDS             2376..>2558
                     /locus_tag="VFY27_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764831.1"
                     /translation="MNRTVAGRIHTQPALSLPEQKICRPLLFPMIALTHVLHITGAGQ
                     EPLQALELSVIAFLQRI"
BASE COUNT          464 a          839 c          735 g          520 t
ORIGIN      
        1 aggcagcaac aggtaagtga ggattgcgta gaggactcgt atcaacgcga atccgtcacg
       61 aacggagctt gtctatgatc ctgctggatg agtggccgtc gcgaaaagac agcacgcgaa
      121 cctcgcctcc gttgcggatg acatccgcgg cgccggctat ctgctcaggg cggtaatccc
      181 ctcccttgac caggacatcc ggcaagacag agccaatcag ccgcgccggc gtgtcctcgg
      241 cgaagggcac gacccactcg accgacgcca ggccggcaag taccgccata cggtcagcaa
      301 ggatattgaa ggggcgaccc tcgcctttca gtcgacgcac ggaatcatcg tcgttgacgg
      361 cgacgacgag gcgatcgccc aggtttgtcg cttcttccag gtatgcgacg tgtcctgcat
      421 gcaggacgtc gaaacagccg ttcgtcatga cgatcttctc tccccgctcc ctgcactccg
      481 caagcaagga caaaagctcc tcctcgctca ccatgccggg accaccctgt ccgtgctggt
      541 gcagggctat gctgagttca gacggggtga cactcgccac gccgatcttg cggactacga
      601 gtcccgcggc gagattcgca agaccgacgg cggaccggaa atcgctgccg cttgcatatg
      661 ccgccgcaaa cgtcgcaatg acggtgtcgc ctgcaccggt cacatcgtag acttcccgcg
      721 cctgcgtgga gaggaagaac ggttcctccc cggcttttac catcagcatg cccttttcgc
      781 tgcgcgtcac cagtaatgct tccagctcca ggtcacggag catctttcgc gcgcgcgcga
      841 ccatgtcgtc atcggacgcc gcggatccgg ccacggcctc gaattccgac tggtttggcg
      901 tgatgacgga cgatccccga taccgcgaaa agtcagtgcc cttgggatcg accatcaccg
      961 gcacgcctgc cagcctgcag gcctcaatca tgctggcgac gtctcccagt gcgcccttgc
     1021 cgtagtcaga gaggacgacg gcgtcgacat ccttgagggc cgccgtaagg gacgctgcca
     1081 gcgacggccc tgccgggtcg ccctgggatt cttcacgatc cagcctgatc aactgctgtc
     1141 cgcggctatg cacgcgggtc ttcgtgatcg tcggacggtg atcggagcgg gaaaaacggc
     1201 aggtgatgcc gcgtgcctcg agcacctcct gtagccgccg cgcctcttca tccctgccta
     1261 caacgcccag aagagtggtc gtgaccccca gggcggccag attcaccgca acgtttgccg
     1321 caccacctgc cctgtcgtcg ctgctacgca catgcaccac cggcaccggc gcctcgggag
     1381 atatccggct ggtgccgccg aacaggtagc gatcgagcat cacatcgccg gcaacgagga
     1441 cgcgggtacg cgaaaaatcc gggatggtaa gagtcacggc gggaatacta ctacaaggcc
     1501 cgcggcacac agaaaggcac aagccgcgcc aggcaacaat gcttcacaag tccgggcgga
     1561 aacgggcttc ccaggcccga ggcagcgccc gagggaagcg ctacgtgttg cctgtcagcc
     1621 gcacttcgac tcgccgcaac tcaggcatgt catgcatccg tccatcatga tcacggcggc
     1681 ggtgctgcac ttggcgcaga gttgtgcgcc ttcgggatac tcgctcttcg aaaatgcgtc
     1741 ctgctgtttt gcggccgctt cgaactcggc ccgcttctgc tcgattagtt tcttctgctc
     1801 ttcgtcaagc ttcggcttcg ccagcaagcc gatcatgatg aggtgctttt cgacgatata
     1861 gccgagctcg gcgatgatcg agggcatgaa cttgccgccg gctttccagt agccgccgcg
     1921 cggatcgaac acggccttga gttcgtcgac gaggaacgtc acgtccccgc ccttgcggaa
     1981 cacggcggag atgattcgcg tcagcgccac gatccactgg taatgatcca ggttcttcga
     2041 gttgatgaag atttcgaacg ggcgtctttt ctcgtgctct gttccctcgt tcaggacgat
     2101 gtcgttgatg gtcacgtaca tcgcatgatc cgacaccggc gtctttacct tgtaggtcga
     2161 gccgatcagc atttccggcc gttccagctt ttcgtgcatc cggatgactt cggcgcgccc
     2221 ggcctcgggt gtctcgcgcc gcggttccgc gctgtcggcc gccgcgtcct tgaccggagc
     2281 ctgtttcacc gcataaccca cgattttgcg gtctattttg gtcttgctca tttcctgttt
     2341 ccctgtgttc cagcgtcccg gcggtggccg tccgcgtgaa ccgtaccgtc gcgggtcgta
     2401 tccacactca gccagctctt tcgctccctg agcaaaagat ttgccggccg cttctttttc
     2461 caatgatcgc gctgactcat gttcttcata tcaccggtgc gggtcaggag cctctgcagg
     2521 ccctagaact ttccgtaata gccttccttc agcgcatc
//
LOCUS       DASUOM010000222         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_17202,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000222 DASUOM010000000
VERSION     DASUOM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17202"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /gene="maiA"
                     /locus_tag="VFY27_04640"
     CDS             complement(<1..643)
                     /gene="maiA"
                     /locus_tag="VFY27_04640"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX5764832.1"
                     /translation="MKLYGYYRSSASYRIRIILNVKGIAWENQPVMLNRDEHRAGTFK
                     AINPTGFVPVLDTGEAILAQSAAIAEYLEETVPAPPLLPEHPVLRAQVRELQHLIGCD
                     IHPLQNLRVLKHLRAEFGQDDEGVEKWCRRWIGAGFEAFEIIAAKRSASGRYSVGDSL
                     SLADAWLVPQLYNARRFGLDLAPFPTIAGIGEHCLALDPIAAAHPDRQPDAPRL"
     gene            831..1538
                     /locus_tag="VFY27_04645"
     CDS             831..1538
                     /locus_tag="VFY27_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764833.1"
                     /translation="MSVSAVAQDVEKKITDAINSDIRSETEVARDPDRSPLETLTFLG
                     LRDDMRVLELLPGGGWYTKILAPVLRENGALYVAIGTDNVSENLVSQHGFDHVNVVDV
                     GVSMERTGPFNTNFMPPFEFGLDDLDMALTFRNMHNFTPEGRANVNTAVFKALKSGGL
                     YGIVDHSRRHMEPMTAENRRRADVVQIMKEVLAAGFEFVDYSDVHYRPDDELRYEVGR
                     KTVTGNTDRFTLLFKKP"
     gene            1560..2342
                     /locus_tag="VFY27_04650"
     CDS             1560..2342
                     /locus_tag="VFY27_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5764834.1"
                     /translation="MRCRPVIGVPADRRILEPHPFHMAGEKYLTAIRDGAGGLPVIVP
                     ALGPSIEADEWLANVDGILLTGSLSNVEPHHYGGEPSREGTLHDRERDQTTLPLARRA
                     LDCGVPLLALCRGFQELNVVLGGTLHQRVHEVPGYGTHKENPEDPVEKQYGPAHYVTL
                     PEGGLLRKLAGSDRVQVNSLHSQAVARLAAGVTVEAVADDGLIEAFRVDSADTFALAI
                     QWHPEWRVTENEFSMSIFGAFGDACRARAADRIKVNHKVLRE"
     gene            2335..3669
                     /locus_tag="VFY27_04655"
     CDS             2335..3669
                     /locus_tag="VFY27_04655"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5764835.1"
                     /translation="MSEKEIFQDWFKKHRIEDVEAIVPDMAGAARGKLLPADKFGSGD
                     MKMPEAVFAQTISGEYVINASNVEDRDMWLKPDATTLRPVPWASDPAASVFLDCFRKD
                     GSPVDTSPRHVLRKVLALYEERGWKPVVAPEVEFYLINPHSDANAEVEPPEGRLGRTD
                     MSRQPYSIDTMNDFDPYINDVYAYCEAQGIRIDTLSQEMGPAQFEINFLHGDAIALAD
                     QVFLFKRTVREAAIEHEMHATFLAKPMSEEAGSALHIHQSIVNIADGSNIFSTESGEP
                     SKYFYSFLGGLQRYMPEAMLIFAPYINSYRRFLNSWASPVNLAWAIDNRTVGLRVPDS
                     SPEARRIENRLAGSDVNPYLALAGSLACGYLGMIEGLEASPPIEGSAYNMPFMLHRHI
                     YSAIEAMKGSDAMRKTLGDSFVTLYCLLKEDEEREFEEIITPWEREILMFNV"
     gene            3874..>4230
                     /locus_tag="VFY27_04660"
     CDS             3874..>4230
                     /locus_tag="VFY27_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5764836.1"
                     /translation="MSVAKTRSAAEWQDLDRDHYLHPFTDYKDLGSKRSRIITRADGV
                     YIFDAAGNKILDGMSGLWCVNAGYGREELVEAAARQLRELPYYNSFFQCAHPPSIELS
                     RMLREVTQPHFNRVFFT"
BASE COUNT          881 a         1238 c         1328 g          783 t
ORIGIN      
        1 agaggcgggg cgcgtctggc tgtcggtcgg gatgtgcggc ggcgatgggg tccagcgcga
       61 ggcagtgttc gccgatgccg gcgatggtgg gaaacggcgc aaggtcaagg ccgaatcgcc
      121 ttgcgttata gagctgcggg accagccagg cgtcggccag ggacagcgaa tcgcctaccg
      181 agtaacggcc ggatgcggaa cgcttcgcgg cgatgatctc gaaggcttcg aaacctgcgc
      241 cgatccacct gcggcaccac ttctcgactc cttcatcatc ctggccgaac tccgcgcgca
      301 agtgcttcag gacgcgcagg ttttgcagcg ggtgtatgtc gcagccgatg aggtgctgca
      361 gctcgcgcac ctgggcccgt aaaaccgggt gctccggcag caagggaggc gctggaactg
      421 tctcttccag gtactcggca atggcagctg actgggccag tatcgcctca ccggtatcca
      481 gcaccgggac aaagcccgtc ggattaatag ccttgaacgt gccggcgcga tgctcatccc
      541 ggttcagcat caccggctgg ttttcccagg caatgccctt cacgttcagg atgatgcgga
      601 tgcggtagga agcgctgctg cggtaatagc cgtaaagttt catgaatttt ccctgacccg
      661 ggctggtcct tcgcaggata caacataaaa ggtgccgcaa aagccgccgc gatgaccggc
      721 ttatgccgat aggttagact tgccgcacaa cgactgaagg cgcgcatgcc gtcgtataca
      781 accgggggtc aaaggtgatt cgtttttgtc tggcagtgtc agttagtttg atgagcgtgt
      841 cggcggtcgc ccaggacgtc gagaagaaga ttaccgatgc aatcaacagc gatataagga
      901 gcgaaaccga ggtcgcgcgc gacccggatc gttcgccgct cgagactttg actttccttg
      961 gcctgcgcga cgacatgcgg gtgctcgagc tgctccccgg cggcggctgg tacacaaaaa
     1021 ttctcgcgcc cgtattgcgc gagaacggcg cgctctatgt ggcgatcggc accgacaacg
     1081 tcagcgagaa cctggtatcc cagcacgggt tcgaccatgt aaacgtcgtc gacgtcggtg
     1141 tcagcatgga gcggacgggt ccgttcaaca ccaatttcat gccgccgttc gaattcggtc
     1201 ttgatgatct cgacatggcg ctgacattca ggaatatgca caacttcacg ccggaaggca
     1261 gggccaacgt caatacggct gttttcaagg ctttgaaatc cggcggcctt tacgggattg
     1321 tcgatcattc gcgcaggcac atggaaccta tgactgccga aaatcgccgt cgtgcggacg
     1381 tcgtgcagat catgaaggaa gtgctggcgg cgggattcga gttcgtcgac tacagtgatg
     1441 tgcattatcg ccccgacgac gagcttcggt atgaagtcgg gcgcaaaacc gtcacgggca
     1501 acaccgatcg gttcacgctc ctgttcaaaa aaccctgagg cagcggttca ggtccgcgta
     1561 tgcgttgcag accagtcatc ggagtaccgg cggaccggcg aatcctcgag cctcacccgt
     1621 ttcacatggc aggagagaag tacctgactg cgatccggga cggggcaggc gggttgccgg
     1681 tgatcgtacc ggcgctcggg ccgagcatcg aagccgacga gtggctcgca aacgtcgacg
     1741 ggatacttct cacgggcagc ctgtcgaacg tcgagccgca tcactacggc ggcgaaccga
     1801 gccgggaagg gacgctgcac gatcgcgagc gggaccagac gaccctgccg cttgcaaggc
     1861 gcgcactcga ttgtggtgtc ccgctgctcg cgctttgccg cgggttccag gaattgaacg
     1921 tggtgctggg gggcacgctg caccagcggg tgcacgaggt gccgggatac ggcacgcata
     1981 aggaaaatcc ggaagatccg gtggagaaac agtatggacc tgcgcactac gtcaccctgc
     2041 cggagggtgg ccttctgcgc aaactggccg gcagcgaccg ggtgcaggtg aactcgctgc
     2101 actcacaggc ggtggcaagg cttgctgccg gagtgaccgt ggaagcggtc gccgatgacg
     2161 ggctgataga ggcatttcgc gtcgactccg ccgatacgtt cgcattggca atacagtggc
     2221 acccagagtg gcgcgtcact gaaaacgaat tctcgatgtc gatcttcggg gcgttcggcg
     2281 acgcctgccg ggcgcgcgcc gcagatagaa tcaaagtcaa ccataaggtg ttacgtgagt
     2341 gaaaaagaaa tatttcagga ctggttcaag aaacatcgca tagaagacgt cgaagcgatc
     2401 gttcccgaca tggccggggc ggcgcgagga aagctgttgc ctgccgacaa gttcggctcc
     2461 ggcgacatga agatgccgga ggcggttttc gcgcagacta tctccggcga gtacgtgatc
     2521 aacgcctcca acgtggagga tcgcgatatg tggctgaagc cggacgccac gacgcttcgc
     2581 ccggttccat gggcgagtga tcccgcggcc tccgtatttc tcgattgctt ccgcaaggac
     2641 ggctcgcctg tcgacacatc gccgcggcac gtgttgcgca aggtgctggc gctctatgaa
     2701 gagagaggct ggaagcctgt cgtcgctccc gaagtcgagt tctacctgat caacccgcac
     2761 tcggatgcga acgcggaggt cgagcctccg gagggccgcc tcggcaggac cgacatgtcg
     2821 cggcagccgt acagtatcga cacgatgaac gacttcgacc cgtacataaa cgacgtgtac
     2881 gcttactgcg aggcgcaggg gatccgcatc gacacgctgt cccaggaaat ggggccggcg
     2941 cagttcgaga tcaacttcct gcacggtgat gccatagcgc tcgccgacca ggtgttcctg
     3001 ttcaagagaa cggtgcgcga agccgctatc gaacacgaga tgcacgcgac gtttctcgcc
     3061 aagccgatgt cggaagaagc cggcagcgct ctgcatatcc accagagcat cgtgaacatc
     3121 gccgatggca gcaatatttt ctcgacggag tcgggagaac cgagcaagta cttctattcc
     3181 ttcctcggcg ggctgcaacg gtacatgccg gaggcgatgc tgatcttcgc cccctacata
     3241 aactcctaca ggcgtttcct caactcctgg gcatcgccgg tcaacctggc gtgggcgatc
     3301 gacaatcgca cagtcggcct gcgcgtacca gactcctcgc ccgaagcgcg ccgcatcgaa
     3361 aaccggctgg cgggctccga cgtcaacccc tatctcgcgc tggccggaag cctggcctgc
     3421 gggtacttag gcatgatcga aggcctcgaa gccagcccgc cgatcgaggg cagcgcatac
     3481 aacatgcctt tcatgctgca ccggcacatc tactcggcga tcgaggcgat gaaggggagc
     3541 gacgcgatgc gcaagacgct cggcgacagt ttcgtcacgc tttattgcct gctgaaggaa
     3601 gacgaggagc gcgagttcga ggaaatcatc accccctggg agcgcgaaat cctcatgttc
     3661 aacgtctgaa ggcgcgaggc tccgccatcg ccacgccgct cgttcatgga ccatgcgaca
     3721 gggatgttca gattcagcgg tgggcgaggg ttcggccgcg atcgattcca gatcatgacc
     3781 gcgagcagaa caagaacctg ccgctggcta tgattcgctc atcacttaaa aaccagccgg
     3841 tgtaaccggt tgttcaagca tcaggaaaca gcaatgtcag ttgcaaaaac gcggtcggct
     3901 gcggaatggc aggatctcga ccgggatcac tacctgcatc cctttaccga ttacaaggac
     3961 ctgggctcaa agcgcagccg catcatcacc cgcgccgacg gggtctatat cttcgatgcg
     4021 gccggcaaca agatcctcga cgggatgtcg ggtctctggt gcgtgaatgc gggttacggg
     4081 cgagaggaac tggtggaggc cgcggcgcgc cagctgcgcg agctgccgta ctacaacagc
     4141 ttcttccagt gtgcgcatcc gccgtccatc gaattgtcgc gcatgctgcg ggaagtcacg
     4201 cagcctcatt tcaatcgggt gttcttcacc
//
LOCUS       DASUOM010000223         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1585464,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000223 DASUOM010000000
VERSION     DASUOM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585464"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFY27_04665"
     CDS             <1..353
                     /locus_tag="VFY27_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764837.1"
                     /translation="RPAMIALFLSGIFLLAGSTSPNAYIAVSCLAMSFLCNQVTEAAF
                     WAAGIGVGGRNAAAACGVMNTGGNAAGFINALLVPFTAATFGWTAAMVTGTLFAFISA
                     ALWFFIRADQPVAD"
     gene            543..2534
                     /locus_tag="VFY27_04670"
     CDS             543..2534
                     /locus_tag="VFY27_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5764838.1"
                     /translation="MRPIPLALTLSALLLATAAFSRPVELDDMYRLRQVSEARISPDG
                     ERIAYTVTTPDLERDEEVSDLWMTNWDGDISLQLTRARESEHHPRFSPDGQQLAFLSD
                     RGNADEGDQVWLLDMRGGEARQVTTVAEGVTDFAWSPDGKRMVLVAEVAEQKDPDDKP
                     APIVIDRLYFKEDVTGYLGTSRYHLFLQDLDKGTIEQLTAGDYDELQPSWSPDGSRIA
                     FVSKRGEDMDRHDNWDVYVMEATPGASAAKVTRNPGADGDADGSWGSGPPAFSADGKR
                     IAYLHGGAEEDIWYGLLQVGVVDTGGSGAALPTEALDRNTLSPRWSQDGRSLRFLLED
                     DQSRQLAEVRLRDGSIRRLTGPEQVVHEFDMSRSGRTVMVMSRSDRPPEVWKLERGQP
                     RQLSRQNAEWLEEVDLSTARTISFKSADDVEIHGMVMSPATRSATGPLPLILRIHGGP
                     VSQYQHEFDFEWQLFTANGYVVAGVNPRGSSGRGYEFQKMLFADWGFADVPDINAAVD
                     YLVEAGLADPERLGVGGWSYGGILTNYMIASTDRFKAAISGAGMSNMLGGYGIDQYVR
                     AWERELGKPWENTEAWLRLSYPFLEADRIETPTLFMCGASDFNVPLAASEQMYQALKS
                     LDVPTELIIYPDQYHSLTRVSFQHDKLRRYIEWHDRYLK"
     gene            2531..3865
                     /locus_tag="VFY27_04675"
     CDS             2531..3865
                     /locus_tag="VFY27_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5764839.1"
                     /translation="MSESHLFYQQASRRPRLERAEGIYLWDVHGKRYLDGSSGAMVSN
                     IGHSNPRVLAAMKQQMDKSTFAYRLHFENDPAEDLASLMASLMPPGLDRIFFVSGGSE
                     AIESCLKLARQYAVVTGQSSRWKVIALYPSYHGSTLGAAAVTGMQQFVEPFRDMIRVM
                     PKIPAATCYLDRDSLSHEERGERYAEYLREEIERQGPETVLAFIMEPVGGASTGALVP
                     PDSWFARIREICREYGILLIADEVMTGAGRTGRFLASEWWDLQPDIIAVAKGFGAGYA
                     PLGAMAAHGRIVSAILDNGGFAHGYTYAGNPLACSAGLAVLQELLEHKLVENADRQGR
                     KLRARLTELMDRYPFIGDVRGKGLLLAFELVRDKDSMQPLDPALNAHLALVDEAYRRG
                     LIIYSRRTRGGLVGDHFMVCPPLIITDAQLDEVMELLTASIDAWARSAGLPL"
     gene            3862..>4773
                     /locus_tag="VFY27_04680"
     CDS             3862..>4773
                     /locus_tag="VFY27_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5764840.1"
                     /translation="MSKVIITCAVTGSIHTPTMSPHLPVTPEEIATQGIEAARAGAAI
                     LHLHARNPETGQPTADPSVFMQFLPRIKEQCDAVINITTGGSSLMTLEERLAAPNLAQ
                     PEMASLNMGTMNFGLFPMKRRYTDWKHDWEPKLLDATKEVAFRNTFADIEQIFHQLGD
                     GYGTRFEFECYDVGHIQTLAFYLREGLIRKPLFVQFVLGVLGGIDAAPENLMHMKATA
                     DRLLGDDYRFSVLCAGRLQIPLATMGAILGGNVRVGLEDSLLIGRRKLARNNAEQVTK
                     IRNILEELGHEIATPAEAREILDLKGAD"
BASE COUNT          908 a         1533 c         1520 g          812 t
ORIGIN      
        1 cgaggcctgc gatgattgcc ctgtttttat cgggaatatt cctgctggcc ggctcgactt
       61 cgccgaacgc ctacatcgcg gtttcctgcc tggccatgag cttcctgtgc aaccaggtta
      121 cggaagccgc gttctgggcg gcgggcatcg gcgtcggcgg gcgcaatgcg gcggcggcct
      181 gcggcgtgat gaacaccggc ggcaacgcgg caggcttcat caacgccttg ctggtgccat
      241 tcaccgcggc aaccttcggc tggaccgcag ccatggtgac cggaacgctg ttcgcgttca
      301 taagcgccgc cctctggttc ttcatccgcg ccgaccaacc cgtcgcggat tgagtgtcgc
      361 gtccggacag gccgctgtag gagcctgcca tgcaggccag ttcgatcagg cttcagcgaa
      421 ctcgcccgca cggcgggctc ctacatttaa ggcgccgtca ggatatggaa ctcgcgcgaa
      481 cgccctcgca ctgcttgcta gagtaccgcc acaacaacgt cactccccgg tgccttgaac
      541 acatgcggcc aattcctctc gccctgactc tttccgctct gctgctcgca acagctgcgt
      601 tcagccgccc tgtcgaactc gacgacatgt atcgcctccg gcaggtaagc gaagcgcgca
      661 tctcgccgga cggagagcgg atcgcctaca ccgtcacgac accggatctc gagcgggacg
      721 aggaggtcag cgacctgtgg atgacgaact gggatggcga tatttccctg cagctcaccc
      781 gcgcccgcga atcggagcac cacccgcgct tcagtcccga cggccagcag ctggcattcc
      841 tttccgaccg cggcaacgct gacgagggcg accaggtatg gctgctcgac atgcgcggcg
      901 gcgaagcccg gcaggtcacc acggttgcag agggcgtcac cgacttcgca tggtcacccg
      961 atggcaagcg catggtgctg gtcgccgagg ttgcggagca aaaggatccg gacgacaaac
     1021 ccgcaccgat agtcatcgat cgcctctact tcaaggaaga cgtcaccggt tacctcggca
     1081 cgtcccgtta ccacctgttc ctgcaggatc tcgacaaggg caccatcgag cagttgacgg
     1141 ccggtgatta cgacgagttg caaccgtcgt ggtcgccgga cggttcgcgc atcgcgttcg
     1201 tcagcaagcg cggggaggac atggaccggc acgacaactg ggacgtctac gtgatggaag
     1261 cgacaccggg ggcgagcgcg gcaaaggtca cgcgaaaccc cggcgccgat ggcgacgcag
     1321 acggcagctg gggcagcggt ccccccgcct ttagtgcgga cggaaagcgc atcgcgtacc
     1381 tgcatggcgg cgcggaggaa gacatctggt acggactcct gcaggtaggg gtagtcgaca
     1441 ccggcggcag cggtgcggcc ctgccgaccg aagcgctcga tcgaaatacc ttgagtcctc
     1501 gctggtcgca ggatggccgc tcgctccggt tcctgctgga ggacgaccag agcaggcaac
     1561 tggcagaagt gcgactgcgc gacggcagca tccggcgcct caccgggccg gagcaggtcg
     1621 tgcacgagtt cgacatgagc cgaagcggcc gcaccgtcat ggtcatgagc cgcagtgaca
     1681 ggccgccgga ggtgtggaag ctggaacgcg gccagccgcg gcaactctca cgacagaacg
     1741 cagaatggct tgaggaggtc gacctttcga ccgcacggac aatcagcttc aagagcgccg
     1801 acgacgtcga gattcacgga atggtgatgt cgcccgcgac ccgcagcgcg accggaccct
     1861 tgccactgat cctgcgcatc catggcggtc ccgtctcgca ataccagcac gagttcgatt
     1921 tcgaatggca actcttcacc gcgaacggct acgtcgtcgc gggcgtcaat ccgcgcggca
     1981 gttcaggccg cgggtacgaa ttccagaaga tgttgttcgc ggactggggg ttcgccgatg
     2041 ttcccgacat caacgcggcg gtggattacc tggtcgaggc agggctcgcc gatcctgaac
     2101 gcctcggtgt cggcggttgg agttacggcg gcatactgac gaactacatg atcgcatcga
     2161 cagatcgctt caaggcggcg atcagcggcg cgggcatgtc gaacatgctg ggcggatacg
     2221 gcatcgacca gtacgtgcga gcctgggagc gcgaactcgg caagccatgg gaaaacaccg
     2281 aggcgtggct gcgcttgtcg tatccgttcc tcgaggccga ccggatagaa acaccgacgc
     2341 tgttcatgtg cggcgcgtcc gacttcaacg tgccgcttgc cgcgtccgag caaatgtacc
     2401 aggcgctcaa gagcctcgat gtgccgactg aactcatcat ctatccggac cagtatcatt
     2461 cgctgacgcg ggtgagcttc cagcacgaca agctcaggcg ctacatcgag tggcatgacc
     2521 gatacctcaa atgagcgaga gccacctctt ttaccagcag gccagccgcc gcccccggct
     2581 cgagcgggca gaaggcattt acctctggga cgtgcatggc aaacgctatc tcgacggctc
     2641 gagcggagcg atggtctcga acatcgggca ttccaatccc cgagtgctcg cggcgatgaa
     2701 gcagcagatg gacaagtcga cattcgccta ccgcctgcac ttcgagaacg accccgccga
     2761 ggacctcgcc tcgctgatgg cgtcgctgat gccgccgggg ctggaccgga ttttcttcgt
     2821 gtctggcggg tccgaggcta tcgaaagctg cctgaagctt gcccgtcagt atgcagtggt
     2881 gaccgggcag tcgagccgct ggaaggtcat cgcgctctat ccttcgtacc acggctcgac
     2941 gcttggcgct gccgccgtaa ccggcatgca gcagttcgtc gaaccgttcc gcgacatgat
     3001 ccgcgtcatg ccgaaaatcc ctgccgcgac ctgttacctg gaccgtgaca gtttgagcca
     3061 tgaagagcgc ggcgagcgct acgcggagta cctgcgcgag gagatcgaac ggcaaggtcc
     3121 ggaaaccgtg ctcgcgttca tcatggagcc tgtcggcggc gcctcgaccg gcgccctggt
     3181 cccgcccgat tcgtggttcg cccgcatcag ggagatctgc cgggagtacg gcatcctgct
     3241 gatagcggac gaagtgatga ccggtgcggg gcgcaccggg cgtttccttg cttcagaatg
     3301 gtgggacctg cagccggaca tcatcgccgt ggccaagggt ttcggtgccg gctatgcacc
     3361 gctcggggcg atggccgcac acggccggat cgtcagcgca atcctggaca acggcggttt
     3421 cgcgcacggc tacacctacg ccggcaatcc gctcgcttgt tccgccggtc tcgccgtcct
     3481 gcaggaactg ctggagcaca agctcgtcga gaatgcggac cggcagggcc gcaagcttcg
     3541 cgcccggctg accgaactca tggaccggta ccctttcatc ggtgacgtcc gcggcaaggg
     3601 gctgctgctt gcgttcgagc tggtgcggga taaagattcc atgcagccgc tcgaccccgc
     3661 gttgaatgca cacctggcac tggtcgacga agcgtaccgt cgcggcctga tcatttattc
     3721 gcggcgcacc cgcggcggcc tcgtgggaga tcatttcatg gtatgtccgc cgctgatcat
     3781 cacggatgca cagctcgacg aagtgatgga actgctcacc gcaagcatcg acgcgtgggc
     3841 gaggtcggcg ggattgccgc tgtgagcaag gtcatcatta cctgcgcggt cacgggctcg
     3901 attcatacgc ccaccatgag cccgcacctt ccagtgacgc cggaggaaat cgccacgcaa
     3961 ggcatcgaag ccgccagggc gggcgcagcg atcctgcacc tgcacgcgcg caatccggaa
     4021 acgggacaac cgaccgccga tccctcggta ttcatgcagt tcctgccgcg catcaaggag
     4081 caatgcgacg ccgtcatcaa catcacgacc ggcggcagct cgctgatgac gctggaagaa
     4141 cgtctcgccg cgccgaacct cgcgcagccg gagatggcct cgctcaacat gggcacgatg
     4201 aacttcggcc tgttcccgat gaaaaggcgc tatacggact ggaagcacga ttgggagccg
     4261 aagctgctgg acgcgacgaa ggaggttgcg ttcaggaaca cgttcgccga catcgagcag
     4321 atttttcatc aactgggcga cggctacggc acgcgcttcg agttcgagtg ctacgacgtc
     4381 gggcacatcc agacgctcgc gttctacctg cgggaggggc tgatccgcaa gccgctcttc
     4441 gtgcagttcg tgctcggcgt gctgggcggc atcgacgcgg cgcctgagaa cctgatgcac
     4501 atgaaagcga ccgccgaccg gctgctgggc gacgattacc gcttctcggt cctctgcgcc
     4561 gggcggttgc agatcccgct cgcgacgatg ggcgccatcc tgggcggcaa cgtgcgcgtt
     4621 ggtctcgaag acagcctgct gatcggccgc cgcaagctcg cacggaacaa cgcggagcag
     4681 gtaacgaaaa tccgcaacat cctggaagag ctcggccacg agatcgccac accggcagaa
     4741 gctcgggaaa tcctcgacct caaaggcgcc gac
//
LOCUS       DASUOM010000224         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_997668,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000224 DASUOM010000000
VERSION     DASUOM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997668"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1233)
                     /gene="recF"
                     /locus_tag="VFY27_04685"
     CDS             complement(169..1233)
                     /gene="recF"
                     /locus_tag="VFY27_04685"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX5764841.1"
                     /translation="MPLGSFRAERFRCLSLVEFELDPKGNLFVGPNGSGKTSLLEAAF
                     FLSRGRSFRSRRRESLIAQGSDEFLVSGRADTDAGSVALGVRAGRTQTEWRIGGSPAP
                     GVADLAELFPAQIIDPEVHKLLEEGPGRRRRFLDWGVFHVDHAFLSNWRRYHKALRQR
                     NAALKVDAADQALAVWERELTVTGEALTAQREGYLARLAAPLARIGESLLQDPITLTH
                     HRGWDSSQSLGEALAEHRTRDRRYRATQIGPHRSDITVLIGDRPAKDRVSRGQQKLVA
                     SALMLAQLDIQEAERPGRSALLLDDPAAELDGENLTRLMSLVRRVPAQLWVTSLKPEI
                     EGLVDAGRVFHVKQGQVMAR"
     gene            complement(1241..2335)
                     /gene="dnaN"
                     /locus_tag="VFY27_04690"
     CDS             complement(1241..2335)
                     /gene="dnaN"
                     /locus_tag="VFY27_04690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5764842.1"
                     /translation="MKFSAERESLLTALQSVIGVVERRQTMPILANLLLAADQGTLSI
                     TATDLEVELVATTAIKSGTDGRLTVPGRKLLDICRSLPEGVTLAFSQDGERLTVKAGR
                     SRFVLATLPAADFPTIEELAQQQSIKLPQADLRRLLDKTHFSMAQQDVRYYLNGMLLE
                     TDGKMLRTVATDGHRLALCEMDLPSKAAGQQVIVPRKGVLELQRLLGTEGDVEVTVGS
                     NHIRAQIGDVRFTSKLIDGRFPEYGRVIPAAPPKAVIADREVLRAALQRTAILANEKY
                     RGIRLALKKNTMTLQAHNPDQEEAEEQVEVSYKGDELEVGFNVGYLLDALAAVDGNEV
                     EIGVTDGNSSCLVRAPGATSARYVVMPMRL"
BASE COUNT          413 a          768 c          735 g          462 t
ORIGIN      
        1 ggcacttctt tgcgggttga cacgggcaag cggcgaaatc cttggccgat gtcccatcgg
       61 ccgcaatcgg accttcgggc aagcaacccg cacgaagcaa ggacgtgcta tgtaacgatc
      121 atggcgtgat cgtcgctgaa tatccgtgat catgcggcga tcatgttgct atcgtgccat
      181 cacttgacct tgtttcacgt ggaacacgcg accggcgtcc accagcccct cgatttccgg
      241 cttcagtgac gtgacccaaa gctgagctgg aacccggcgg acaagggaca tcagacgggt
      301 gagattctcg ccgtcaagtt ccgcagccgg atcatcgagc aggagggcgg atcgcccagg
      361 cctttcggct tcctgaatgt ccagctgggc cagcatgagg gctgaagcga cgagcttctg
      421 ctggccgcgg gacacgcgat ccttggccgg ccgatcgccg atcaggactg tgatgtcgct
      481 gcggtgaggg ccgatctggg tggcgcgata ccgccgatcg cgtgtgcgat gttccgccag
      541 agcctcccca agtgactggc ttgaatccca gcctcggtga tgcgtcagcg tgatgggatc
      601 ttggagcagg ctttcaccga tacgagccag aggtgcggct agcctcgcca ggtacccttc
      661 tcgctgcgcg gtcaatgcct cgccggtgac cgtcaactcc cgctcccaaa cggcaagggc
      721 ttggtccgct gcgtctacct tcaatgccgc attccgttga cgcagggctt tgtgatagcg
      781 gcgccagttc gaaaggaagg catgatccac gtggaacacg ccccaatcca gaaaacgccg
      841 gcggcggcct ggaccttcct ccaacagctt gtgaacttcg ggatcgatga tctgcgccgg
      901 gaacaattcc gccagatcag cgaccccggg ggcgggagac ccgccgatac gccattccgt
      961 ctgcgttcgg ccggccctga cgcccaaggc aacggacccc gcgtcggtat ccgcgcgccc
     1021 actcaccagg aattcatcgg agccctgcgc gatcagcgat tcacgtcgcc tcgatcgaaa
     1081 agaccggccg cggctaagga aaaacgccgc ttcgagcagg ctggtcttgc ccgagccatt
     1141 cgggccgaca aacagattgc ctttaggatc gagctcgaac tcgaccagcg acaggcaacg
     1201 gaaccgctcg gcgcggaaac tgccgagtgg catgcccgac ttacaggcgc atcggcatca
     1261 cgacataccg ggcgctggtc gcacccgggg cgcgtacgag gcagctcgaa ttgccgtccg
     1321 tcacgccgat ttcgacttca ttgccgtcga ccgctgccaa ggcgtccagg aggtacccga
     1381 cattgaagcc cacctcgagc tcgtccccct tgtaactgac ttccacctgt tcctcggctt
     1441 cttcctgatc cgggttgtgc gcctgcagcg tcatggtgtt tttcttgagc gcgagccgaa
     1501 tgccgcggta cttctcgttg gccaggatcg cggtgcgctg cagggccgcc cgcaggacct
     1561 cgcggtccgc gatgaccgcc ttcggcggcg ctgccggtat gacccggccg tattccggga
     1621 atctgccgtc gatcagcttg gatgtgaagc gaacgtcgcc gatctgggcg cgaatgtgat
     1681 tgcttccgac cgtaacttcc acgtcgccct ccgtgccgag cagccgctgc agttccagca
     1741 ctcccttccg cggcacgatc acttgctgac ccgccgcctt gctcggcagg tccatttcgc
     1801 acaatgccag acgatggccg tcagtcgcga ccgttcgaag cattttcccg tccgtttcca
     1861 gcaacatgcc gttcaagtaa tagcgcacgt cctgctgcgc catcgagaaa tgggtcttgt
     1921 cgagcagcct gcggaggtcc gcctggggaa gcttgatgct ctgctgctgc gcaagctctt
     1981 cgattgtcgg gaagtcggcg gcggggaggg tggcgagcac gaatcggctg cggcctgcct
     2041 tgacggtgag cctttctccg tcctggctga acgccagcgt cactccttcc ggcaacgagc
     2101 ggcagatgtc gagcaacttg cggccgggaa cggtcagccg gccgtcggtg cccgacttga
     2161 tcgccgtcgt cgcgacgagc tccacctcga ggtccgtcgc cgtgattgaa agcgttccct
     2221 ggtccgcggc gagcaggagg ttcgcgagga tcggcatcgt ctggcgtcgc tcgaccacgc
     2281 cgatcacgga ctgcagggcc gtgagcaggc tttcgcgttc agcgctgaat ttcatcgggg
     2341 cacccggtgt gttgtgcggt tctgttcttg caggaatc
//
LOCUS       DASUOM010000225         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_987383,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000225 DASUOM010000000
VERSION     DASUOM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987383"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2178
                     /locus_tag="VFY27_04695"
     CDS             50..>2178
                     /locus_tag="VFY27_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5764843.1"
                     /translation="MTTEKQSPNLTMVVHLTSPDRRYDSIYLRNYATLNIKDELARIP
                     GVGQAATFGAGDYAMRVWIDPDQAAARGLTAGDIVRSIREQNVQVSAGQLGAPPTPEV
                     SDFLVSINAKGRLVTEEEFADIVLKTGSDGQVTRLEDVARLELGASEYSLRSLLDNEE
                     AAAIVIFQAPGSNALELSEAVRAKMAELAEVFPEGVAYDVVYDPTVFVRDSIRSVVKT
                     LLEAVVLVVLVVILFLQTWRASIIPLLAVPVSIVGTFAVLFLLGFSINTLTLFALVLA
                     IGIVVDDAIVVVENVERNISEGLAPLAAAHQAMREVSGPIIAISLVLCAVFVPMAFLD
                     GVTGQFYRQFAVTIAVATVISAFNSLTLSPALAAALLKPHDAPRDAPTRLIDWLLGWV
                     FRPFNRFFNRSSESYQGAVTKSLRRRGAVFAVYAVLLVATGVMFQLVPGGFIPTQDKM
                     YLIGGVQLPEGASIDRTDAVVRKVSDIALSTEGVVDAVAFPGLNALQFNNTPNTGTIF
                     FGLADFDTRRRSAEEITAELNMKFASVQEAFSFAFMPPAILGIGTGSGYSLYVQDRAG
                     HGYGELQKAVTGLSGALSQVPGMGYAISSYQSNVPQLDAEVDRAQAKAQGVALTDLFE
                     TLQVYLGSAYVNDFNRFGRTYKVMAQADASFRNSPDDIGKLKTRNVDGEMVPIGSMVH
                     IEESFGPDPVIRYNGYPAADLMGEADPR"
BASE COUNT          379 a          661 c          699 g          439 t
ORIGIN      
        1 tgtcaaccag gctctggccc ggctccccga ggacgtcagg cgactcggcg tgaccactga
       61 gaagcagtcg ccgaacctga ccatggtcgt gcacctgacg tcgcccgacc gccggtacga
      121 cagcatctac ctgcgcaatt acgcgacgct caacatcaag gacgagctgg cgcggatccc
      181 cggcgtcggg caggctgcga cctttggtgc cggcgattac gcgatgcgcg tgtggatcga
      241 cccggaccag gccgctgccc gcggcctgac ggcgggcgat atcgttcgct cgattcgtga
      301 gcagaacgtg caggtttcgg ccggacagct cggggcccca ccaacgccgg aggtgtcgga
      361 tttcctcgtg tcgatcaatg cgaagggccg gctcgtcacc gaggaggagt ttgccgacat
      421 cgtgctgaag accggcagcg acggccaggt aacgcgcctc gaagacgtgg cccggctgga
      481 gctcggcgca tcggaatatt cgctgcgttc gcttctggac aacgaagagg cagcggccat
      541 cgttatcttc caggcgcccg gctcgaacgc actggaactt tccgaggcgg tacgcgcgaa
      601 gatggcggag ctggccgagg tgttcccgga aggcgttgcc tatgacgtgg tatacgaccc
      661 tacggttttc gtccgcgact ccatccgttc cgtcgtgaag acactgctgg aagcggtagt
      721 gctcgtggtc ctggtcgtta tcctgttcct ccagacctgg cgcgcctcga tcattcccct
      781 gctggcggtt ccggtatcga tcgtgggtac gttcgccgtg ctgttcctgc tgggcttctc
      841 gataaacacg ttgacgttgt ttgcgctcgt actggccata ggcatcgtgg tggacgatgc
      901 catcgtggtg gtcgagaacg tcgagcgcaa tatcagcgaa ggcctggcgc cccttgctgc
      961 cgcgcaccag gcgatgcgcg aagtgagcgg gccgatcatt gcgatctcgc tggtcctttg
     1021 cgcggtgttc gttccaatgg cttttctcga tggagtaacc gggcagttct accggcagtt
     1081 cgcggtgacg atcgcagtcg cgacggtcat ctcggcgttc aactcgctga ccttgtcgcc
     1141 ggcgctggcc gccgcgctac tgaagccgca tgatgctccg cgggatgctc cgacgcggct
     1201 gatcgactgg ttgctcggct gggtctttcg ccccttcaat cgcttcttca atcgcagctc
     1261 cgaaagctac cagggtgcgg taacgaagag cctgcgccgg cgcggcgcgg tattcgcggt
     1321 gtacgcagtc ctgctggttg ctacaggcgt gatgttccag ctggttcccg gcgggttcat
     1381 cccgacgcag gacaagatgt acctgatcgg cggcgtacag ctgccggaag gggcatctat
     1441 cgatcggacc gacgcagtcg tgcgcaaggt gagcgacatt gcgttgtcga ccgagggcgt
     1501 ggtcgacgcc gtcgccttcc ccggcctcaa tgcattgcag ttcaacaata ctccgaatac
     1561 gggcacgatt ttcttcggtc tcgcggattt cgacacgagg aggcgcagtg cagaagaaat
     1621 cactgccgag ctgaacatga agtttgcatc ggtgcaggaa gcattcagtt ttgctttcat
     1681 gccccctgcc attctcggca tcggaaccgg ctccgggtac tccctttacg tgcaggatcg
     1741 tgcgggtcac ggctatggag aactgcagaa ggcggtaacg ggcctttccg gcgcgcttag
     1801 ccaggttccc ggcatgggct atgcgatcag ttcgtaccag tcgaacgtgc cgcagctgga
     1861 cgcggaggta gatcgtgcgc aggcgaaagc gcagggagtc gccctgaccg acctgttcga
     1921 gaccctgcag gtctatctcg gttccgccta tgtgaacgac ttcaaccgct tcggccgcac
     1981 ctacaaggtc atggcgcagg ccgacgcgag ctttcgtaac tcgcccgatg acataggaaa
     2041 gctgaagacg cgcaacgtcg acggcgagat ggtaccgatc ggcagcatgg tgcatatcga
     2101 agagagcttc ggtcccgacc cggtgatccg ctacaacggg tatcccgctg cggacctcat
     2161 gggtgaggct gacccccg
//
LOCUS       DASUOM010000226         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1176449,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000226 DASUOM010000000
VERSION     DASUOM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176449"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFY27_04700"
     CDS             complement(<1..249)
                     /locus_tag="VFY27_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764844.1"
                     /translation="MIHGIDRLFGGLPAWQTSLWCVLLVVFVGVVDTITGAELSFSVF
                     YLAPVSVAAWYGNKTLTFVIATLSSLVWLAIEYSTHGPY"
     gene            complement(421..945)
                     /locus_tag="VFY27_04705"
     CDS             complement(421..945)
                     /locus_tag="VFY27_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764845.1"
                     /translation="MLKRLRIGEVKSYERVQTVSGSETMTRTQACRFRELQTTRFEGK
                     GRRRKAKPHYSLREAARLLRTEVDEILLSAASGRLQCFVTAAGLRGHWRTTGQSEPSL
                     PAEPSAIPEYLALSPADCRQIKDYGSVNVYDLEYPADSVPDGGPVSTAARFILREPMW
                     VDRARIVLKHPLPK"
     gene            1204..>2015
                     /locus_tag="VFY27_04710"
     CDS             1204..>2015
                     /locus_tag="VFY27_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764846.1"
                     /translation="MKRTSSLRAVVVPFCTSLLLAQSAFADITLEEKFSVRGEGLMSF
                     ANMSGTSTMSISDDKASTKGEVKMESRLARMVAGKLGETAEIVRLDQGKLYQLDMKDR
                     RYTEISLAEQRAELERAMGEAREAQQQQPMPVDESQCEWSDPVTEVTRGESESIAGFD
                     AEQLKIAATQSCTDRSTGQVCDFSITLEQWLTPEFSDEATSFYRSYAEQMGFDASGSG
                     EITQRAESLLGRYPGLWAEIAEAAGDVNGYPVRSRFALEIGGSQCEQGKQNTG"
BASE COUNT          415 a          596 c          651 g          353 t
ORIGIN      
        1 gtatggtccg tgcgttgagt actctatggc gagccacacc agactcgaca gggtcgcgat
       61 aacgaaggtg agtgtcttgt tgccgtacca tgccgcgacg gagacgggcg ccaggtaaaa
      121 gacggagaaa gacagttcag cacccgtgat cgtgtcgacg acgcccacga aaacgacaag
      181 caacacgcac cagagcgacg tttgccacgc cggcaaaccg ccgaaaaggc ggtcgatgcc
      241 gtgaatcatg ctgactccac aagtaatttc accggacccc ggtgcaggca gatcgattat
      301 agggaatggc tgtcgatcac cgtttccctt tgcacattat gacaggttcc accgtcctgg
      361 cccggtggct cgctggacag cgcgagttga agccttaagg agtaagcagg cgcgctgtgg
      421 ttacttcggc agggggtgct tcaggacgat gcgcgcccgg tcgacccaca tcggctcgcg
      481 gagaatgaaa cgggctgccg tggagacagg gccgccgtca ggtaccgagt ccgcgggata
      541 ttccaggtcg tagacgttta cgctgccgta gtccttgatc tgccggcagt cggcggggga
      601 cagtgccagg tattccggta ttgcggaggg ctccgccggc aggcttggct cgctttgacc
      661 ggtagtccgc caatgtccgc gcaggccggc cgcggttacg aagcattgca acctgccgct
      721 cgcggcggag agcaggatct catcgacttc cgtcctcagg aggcgcgctg cctcgcgcag
      781 ggagtaatgc ggctttgcct tgcgtctcct gcccttgccc tcgaagcggg tggtttgcag
      841 ctcccggaaa cggcaggcct gggtgcgtgt catcgtctcg gacccgctga cggtctggac
      901 gcgctcgtac gatttgacct ccccgatgcg aagcctcttc agcaatgtat cgaacctgac
      961 cacgaatctg acccctggcg aaaaggacta cccgccagct taaggaaagg gcagcaggag
     1021 tctgtgatca acttcacatc ggatcgtacg cctcgcgttc gttgtcgccg ataagccgcg
     1081 cattggccgt tgtagtacga tccgggctga gcggcccgtg agtcgcgccc ggaatcagcg
     1141 gcgcccctcc gggacagcag aaccggccag caagccggca ttcgaatttc tggagatact
     1201 caaatgaagc gcacttcgtc gttgcgagca gtcgttgtcc ccttttgtac gtcgctgctg
     1261 ttggcgcagt cggcgttcgc cgacatcacc ctggaggaga agttctcggt acgaggcgaa
     1321 ggcctgatga gcttcgccaa catgtccgga acgtccacca tgtccatttc cgatgacaag
     1381 gcgagtacga aaggcgaagt gaagatggaa tcacgcctgg ccaggatggt agccggcaag
     1441 ctgggagaga ccgcagagat agtcaggctg gaccagggaa agctgtacca gctcgacatg
     1501 aaggacaggc gctatacgga aatatccctg gcggagcagc gcgcagaact cgaaagggcg
     1561 atgggagagg cgcgcgaagc gcagcagcaa cagccgatgc cggtggacga gtcgcaatgc
     1621 gagtggtcgg acccggtgac ggaggtcacc cgtggcgaga gcgagtcgat cgcaggcttc
     1681 gacgcggaac aactgaagat cgcggccacc cagtcatgca cggaccggtc caccggccag
     1741 gtctgcgatt tctccatcac cctcgagcag tggctgacgc cggaattcag cgacgaggcc
     1801 acctccttct atcgctccta tgccgaacag atgggcttcg acgccagcgg ttccggagaa
     1861 atcacccagc gcgccgaaag cctgctgggc cggtacccgg gcctctgggc cgagatcgcc
     1921 gaggcggcgg gtgacgtcaa tggttacccg gtcaggagcc gcttcgccct ggagatcggc
     1981 ggctctcagt gcgagcaggg taagcaaaac accgg
//
LOCUS       DASUOM010000227         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_697935,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000227 DASUOM010000000
VERSION     DASUOM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697935"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFY27_04715"
     CDS             <1..442
                     /locus_tag="VFY27_04715"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5764847.1"
                     /translation="FFEWVVNEKIWPQYILHSAEDMQRFESLRRRGMFADDNPSCLIV
                     LGQYSTQRMGDPAAIAAFLSTVDVNYCSWTVCCFGPHEHQAMLATHAEGGHVRIGFEN
                     NLVLVDGSPAADNAALVAQFTAAIERSARRLATADEVRETFMFR"
     gene            465..1640
                     /locus_tag="VFY27_04720"
     CDS             465..1640
                     /locus_tag="VFY27_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5764848.1"
                     /translation="MRSIAVCSFVIFALAAGVTAAEERPRARDIGLAVGIFQTGENNA
                     ITDVDGVLVGHATRIEGDDIRTGVTAIIPAPGNLYTDPVPAWIHVGNGYGKLIGETQV
                     REFGEIETPILLTCTLCVWSAANALKEWVYEQPGMGEHTVNPVVGETNDANVNNMWAD
                     PVRRDEVFAALDGASDGPVAEGSVGAGTGTQAFGWKGGIGTSSRVLPETLGGYTIGVL
                     VQTNYGGVLTMNGAPVGRELEDYSYRTDLDDSSAKAHPDDGSIMMVVATDAPLTARSL
                     DRLAMRAMMGLARTGSFASNGSGDYVIAFSTHPDVRRPRGSDAPVATPSLTNEAMSPL
                     FAATAEATEEAIYNAILKATTVESSRGRMEAIPLDEVKRILKKYNVLNWDQSLSPRR"
     gene            1851..>3450
                     /locus_tag="VFY27_04725"
     CDS             1851..>3450
                     /locus_tag="VFY27_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5764849.1"
                     /translation="MATNATTVPNAESPSRTMDDRRAQMYPILTDAQLDELSSHGSEE
                     TFGQGEMLWDVGDRNTRFYVVLEGELEIVRRDPFGNETVLVVHRRGSYAGETALLSGR
                     AAMVAGRAGTRLKVLSIPVERLRDLMVSHSQLGELIMRSFILRRVRMVTDQVSDLLLV
                     GSRHSADTMRLQDFLTRNAQPYRFFDLETSGDMCALLSRCDFGPDDTPLIITSKGEPL
                     KNPSLRTLATRLGISLALDAQKVHDVAVIGAGPAGLAAAVYAASEGLDVIVVDSVAPG
                     GQAGTSSRIENYLGFPTGISGQALAGRAFMQAQKFGARIEIPARLTGMHCGNPFHELE
                     LDNGERIRSRIVVIASGARYRKPALERLETFEGAGVYYGATYLESKLCHGQEVAIVGG
                     GNSAGQAAVFLAESADKVYVLVRSEGLAASMSRYLIRRIETTRNIELLTRTEVTALEG
                     ASKLDGVRWRNGKSSAETSRPVRHLFIFIGASPNSEFIAGSIATDQNGFICTGTEVTR
                     PETEHPWTLSRPPYPLETSCPGIFA"
BASE COUNT          660 a         1059 c         1098 g          633 t
ORIGIN      
        1 gttcttcgag tgggtggtga acgaaaagat ctggccgcaa tacatcctgc actccgccga
       61 agatatgcag cgtttcgagt cgctccggcg tcgaggcatg ttcgccgacg ataatccttc
      121 atgcctaatc gtgctcggac agtattcgac acaacgtatg ggcgacccgg ctgcaatcgc
      181 cgcgttcctg tcaaccgttg acgtgaatta ttgctcctgg accgtctgct gtttcggtcc
      241 gcacgagcat caggcgatgc tcgccacaca tgccgagggc ggacacgtgc ggatcggctt
      301 cgaaaacaac ctggtgctgg tcgatggaag cccggcagca gataatgccg cgctggtcgc
      361 gcaatttacc gctgcgatcg aacgctccgc gcggcgtttg gcgacggccg acgaggtgcg
      421 cgagaccttc atgttccgat agcaaagtct ttaccggagt tgtcatgaga tcgattgcag
      481 tttgctcatt tgtcatattt gccctggctg ccggcgtcac cgccgcagag gaacggccgc
      541 gcgcccgcga tatcggcctc gcggttggca tatttcagac aggcgagaac aacgctataa
      601 ccgatgtgga cggggtactg gtcggacatg caacccgcat cgaaggcgat gacattcgca
      661 ccggcgtcac ggccatcatt ccggcgccgg gaaatctcta cacggacccc gtgccggcgt
      721 ggattcatgt gggtaacggt tacggcaagc tgatcggtga aacgcaggtc cgcgagttcg
      781 gcgagatcga aacgccgata ctgctgacct gcacgctatg cgtctggagt gccgccaacg
      841 cgctcaagga gtgggtgtac gaacaaccgg gcatggggga gcacacggtc aacccggtgg
      901 tcggcgaaac caacgacgca aacgtcaaca acatgtgggc cgacccggtc cggcgagacg
      961 aggtcttcgc ggcgctcgat ggagcgagcg acggtccggt tgccgaggga agcgtcgggg
     1021 ctggcacggg cacgcaggcg ttcggctgga aaggcggtat cgggacgagt tcaagggtgc
     1081 tgccggaaac gctcggcggt tacacgatcg gcgttcttgt gcagacgaac tacggaggcg
     1141 tgttgacgat gaatggcgcg cccgtcgggc gcgaactcga ggattactcc tatcgcacgg
     1201 accttgacga ttcgtcggcc aaagctcatc ccgatgacgg ttcgatcatg atggtggttg
     1261 cgaccgatgc gccgctcacc gcacgcagcc tggatcgtct cgcgatgcgg gcgatgatgg
     1321 gcctcgcccg caccgggtct ttcgcgagca acggttcggg cgattacgtg attgccttct
     1381 cgactcaccc cgacgtccgg cggccgcgtg gcagcgatgc tccggtcgca acaccgtcgc
     1441 tgacgaacga agcgatgtcc ccgctgttcg cggcgaccgc agaggcgacc gaagaagcca
     1501 tctacaatgc aatcctcaaa gccacgaccg tagaaagctc ccgcggcagg atggaagcga
     1561 tcccgctgga cgaagtcaag cgcatcctca agaaatacaa cgtcctgaac tgggaccagt
     1621 cgctatcacc ccgacgctga ccctgccatg cgggcgagtt agctgatgtc aatcccactc
     1681 gcccgcatcc gccgtcctca agggtacctt accccccgat agcggcgcct gttccgttaa
     1741 acgtgggctc ctacacaccc gccgccgaga ccctatcaac cgcgcaaacc ggcgggtaac
     1801 atcaatcgtc ctgctgtcac gcctcgttcg taccggagga cgctcccgcc gtggccacaa
     1861 acgcaaccac agtaccgaac gctgaatcgc cgtcccgcac gatggacgac cgccgcgcgc
     1921 agatgtaccc gattctcacc gatgctcagc tcgatgaact ttcgtcgcat gggagcgagg
     1981 aaacgttcgg gcaaggggaa atgctctggg acgtcgggga tcggaatacg cgcttttacg
     2041 tcgtgctcga aggcgagctc gagatcgtgc gccgcgaccc cttcggcaac gagaccgtgc
     2101 tcgtggtgca ccgccgcggc agctatgccg gtgagacggc cttgctctcc ggacgagcgg
     2161 cgatggtcgc cgggcgagcc ggcacgaggc ttaaggtact ttcgataccg gtggagcggc
     2221 tccgcgacct catggtgtct cactcgcagc tgggcgagct catcatgcga agttttattc
     2281 tccggcgcgt gcgcatggtc accgaccagg tcagcgatct gctgctggta ggctcccgtc
     2341 attctgcgga tacgatgcgc ctgcaggact tcctgacgag gaatgcccag ccctacaggt
     2401 ttttcgatct cgagacgtcg ggcgacatgt gcgcgctcct gtcacgttgc gactttggcc
     2461 ctgacgatac gccgctgatc atcacgagca agggcgagcc gctcaagaat ccctcgctgc
     2521 gcacgctggc gacgcggctc gggatttcgc tggcgctcga tgcgcagaag gtgcatgacg
     2581 tagcggtaat cggggctggt cctgccgggc tggcggccgc ggtttatgcg gcatcggagg
     2641 gtctggacgt gatcgtcgtg gactcggtcg ctcctggcgg ccaggctggc accagctcca
     2701 ggatcgaaaa ctatctcggc tttccgaccg gaatctccgg gcaggcgctg gcgggccggg
     2761 cattcatgca ggctcagaag ttcggcgccc ggatcgaaat cccggcacgc ctgaccggta
     2821 tgcactgcgg caatcccttt cacgaactcg aactcgacaa cggcgagcgc atccgcagtc
     2881 gcatcgtcgt catcgcatcg ggggcgcgat accgtaagcc ggcactggag cgcctggaaa
     2941 cttttgaggg cgccggtgtt tactatggcg cgacgtacct cgagtcgaag ctctgccacg
     3001 gacaggaagt cgccattgtc ggtggcggaa attccgccgg gcaggcagca gtgttcctcg
     3061 ccgaaagcgc ggacaaggtg tatgtgctgg tgcgatccga aggtttggct gccagcatgt
     3121 cgcgatacct gatccgccgt atcgagacga ccaggaatat cgagttgctg acacggaccg
     3181 aggtgaccgc gctggaagga gcttcaaagc tcgatggggt ccggtggcgg aacggcaagt
     3241 cttcggccga gacgtcgcgg ccggtaaggc acctgttcat cttcatcggc gcgtcgccta
     3301 acagcgagtt catcgccggt tcgatcgcga ccgaccagaa cggcttcatt tgcacgggga
     3361 cggaagtgac gcgtccggaa acggagcatc catggacact gtcgcgcccg ccgtacccgc
     3421 tggagaccag ttgcccgggc atcttcgccg
//
LOCUS       DASUOM010000228         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1577758,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000228 DASUOM010000000
VERSION     DASUOM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577758"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFY27_04730"
     CDS             <1..896
                     /locus_tag="VFY27_04730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5764850.1"
                     /translation="IALLPDLANQQHYEVPPEFFEIVLGKRCKYSCCYWPANVRSLDE
                     AEEAALDMTCVRSRLEDGMSVLDLGCGWGSLSLWIAERYPACAVTAVSNSRSQRSSIE
                     QRAISKGLGNLRVITADMNHFASDRKYDRIVSVEMFEHMRNYRALFARLASWLERDGY
                     FFMHVFCHHARAYEYVDAGPGDWMSRHFFSGGMMPSRDLPLRFQDHLTLKDQWDWTGH
                     HYRKTADAWLANMDANRSSILPILEEVYGRQQAQQWWMRWRMFFLAVSEMFGSHGGRE
                     WLVAHYLFQPVPAVRYRQPGR"
     gene            914..1441
                     /locus_tag="VFY27_04735"
     CDS             914..1441
                     /locus_tag="VFY27_04735"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HEX5764851.1"
                     /translation="MAFKVGWLSSVIGAVNEMPLLGPAVILAAVAIHLQAVHAPLREL
                     MLILLTGAAGLFWDSLMIAAGWLSYPTGTLVAGLSPYWIVAMWMLFATTFNLSIRWLR
                     SRILLAALFGALSGPASYYAGEKFGAVTFQDFQAAMLGLAVTWGALLPLLLLLARRLD
                     GVTVPQAVARPRSIA"
     gene            1438..2241
                     /locus_tag="VFY27_04740"
     CDS             1438..2241
                     /locus_tag="VFY27_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX5764852.1"
                     /translation="MIDPVAAVTAFGAMLLLALATWIVSVPRKDVSIVDGLWSLMFLA
                     GIVVYLGFLDTLTPRGIVVTALVAVWAIRLSAYIFWRNHGEPEDRRYRRIRERNSPGF
                     EIKSIYLVFGLQAALAGFIALPLLAAVTGGAPLGLLDVAGTLLWLIGFSFEALGDWQL
                     ARFRANPANEGRVLDSGLWRYTRHPNYFGEFCIWWGFYLFAVAAGGWWTVLSPALMSL
                     LLLKVSGVGLLEQDMHRRRPGYVQYKWETNAFFPGPKRHPRNRESFDPR"
     gene            2280..2834
                     /locus_tag="VFY27_04745"
     CDS             2280..2834
                     /locus_tag="VFY27_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX5764853.1"
                     /translation="MAIFPATPAAPVAILLLAMAVGGCAARNEPLPTVPYVDLEKFMG
                     NWYVIASIPTFIEKDAYNAVETYELRKDGAIATTFVFRQGGFGGKEKAYTPVGYVEDK
                     ETNAVWGMQFIWPIRADYRIVYLAEDYSRTIIARNKRDFVWIMARTPAISAGDYEQMT
                     RLIEAMGYDLAKLRPVPQRWQADQ"
     gene            2857..>3687
                     /locus_tag="VFY27_04750"
     CDS             2857..>3687
                     /locus_tag="VFY27_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5764854.1"
                     /translation="MRIAIVGTGIAGNVVAHRLHPAHDITVFESASRIGGHANTIEVV
                     SGDATLKLDTGFMVFNERTYPKFCALLKELEVGSQDTAMSFSVRRETSGLEYCGSSLN
                     ALFAQRANLLRRSFHRMLRDIVRFNRAASEGLDDIAESTTLGEYLEAGQYSREFARDY
                     LVPMGAAIWSNPPDTLPAMPARFFVRFFHNHGLLTFRDRPQWRVIRGGAETYVSKLVA
                     GHQHRIRLNSPVNSIARRGGRVVVRSCGRSESFDRIFIACHSDQALRLLEDASGAERE
                     V"
BASE COUNT          655 a         1113 c         1164 g          755 t
ORIGIN      
        1 cgatcgccct gctccccgat ctcgcgaacc agcagcatta cgaagtccca ccggagttct
       61 tcgaaattgt tctcggtaag cggtgcaagt acagctgctg ttactggcca gctaacgtgc
      121 gctcgctgga cgaagccgaa gaagcagcgc tcgacatgac ctgtgtccgt tcccgccttg
      181 aagatggcat gtcggttctg gatctcggtt gcggctgggg ctcgttgtcg ctatggattg
      241 cggagaggta ccccgcctgc gcggttactg cggtttcgaa ctcccgttca cagcgaagct
      301 cgatcgagca acgcgcgatc agcaaaggcc tcggcaatct gcgggtaatc accgccgaca
      361 tgaaccactt cgccagcgac cgaaagtacg accgcatcgt atcggtggag atgttcgagc
      421 acatgcgcaa ttatcgagcg ctgttcgcgc ggctcgccag ctggctcgag cgcgatggat
      481 atttcttcat gcacgttttc tgccaccatg cccgggcgta cgagtacgtc gacgcgggac
      541 cgggtgactg gatgagtcgg cattttttct ccggcggaat gatgccgagc cgggacctgc
      601 ctcttcgttt ccaggaccat ctgacgctaa aggatcagtg ggactggaca ggtcaccatt
      661 atcgtaagac cgccgacgca tggctcgcga acatggatgc caaccgcagc agcatcttgc
      721 cgatcctcga agaggtctac ggcaggcagc aggcacagca gtggtggatg cgctggcgga
      781 tgttcttcct cgctgtcagc gagatgttcg ggtctcacgg cgggcgtgaa tggctggtag
      841 cgcactacct gtttcagccc gtgcccgccg ttcggtatcg ccaacccggg cgatgaaaat
      901 cctggtaaac gcagtggcct tcaaagtcgg ctggctctcc agcgtgatcg gcgcggtcaa
      961 tgaaatgccg ctactcgggc cggccgtgat cctggccgca gtagcgattc acctgcaggc
     1021 ggtgcacgcg ccgttgcggg aactgatgtt gatcctcctg accggagcgg cgggactgtt
     1081 ctgggacagt ctcatgatcg cggccggctg gttgagctat cccacgggaa ccctcgtcgc
     1141 cgggctgtct ccctattgga tcgttgcgat gtggatgctg ttcgccacca ctttcaatct
     1201 ctcgattcgc tggctccgct cgcggatctt gctggccgct ctgttcggcg cgttgtccgg
     1261 gccggcgtcg tattacgccg gggagaagtt cggtgccgtc acgtttcagg acttccaggc
     1321 cgcgatgctg gggctggctg tcacctgggg cgcgctcctg ccgcttctgc tcctgctcgc
     1381 caggcgactt gacggcgtga ccgtgccgca ggccgtcgcc cggcccagga gtatcgcatg
     1441 atcgatcccg ttgccgctgt caccgccttc ggcgccatgc tcttgctcgc cctggcgacc
     1501 tggatcgtga gtgtgccgag aaaggacgtc agcatcgtcg atgggttgtg gtctttgatg
     1561 ttcctcgcgg gaattgtcgt ctacctcggc tttctggata cgctcacgcc acgcggcatc
     1621 gtggtgaccg cgctggtggc cgtatgggcg atcaggctct cggcctatat tttctggcgc
     1681 aatcacggcg agccggaaga tcgccgttat cgcaggataa gagaacgaaa ctcgcccgga
     1741 ttcgagataa agagcatcta cctggtgttc ggcctgcagg cggcactggc ggggttcatt
     1801 gcattgccgc tgctggctgc ggtcaccggc ggcgcgcccc tcggactgct cgatgtcgca
     1861 ggtacgctgt tgtggctgat cggcttcagc ttcgaagccc ttggcgactg gcaactggcg
     1921 cgcttccgcg ccaatcccgc gaacgaaggc cgggtgctcg acagcggctt gtggcgctac
     1981 acacggcacc cgaactattt tggcgagttc tgcatctggt ggggctttta cctgttcgcg
     2041 gttgccgccg gcggctggtg gaccgtgctg tctccggccc tgatgagcct gctgcttctc
     2101 aaggtgtcgg gcgtcggact gctggagcag gacatgcacc ggcgtcgtcc cggctatgtg
     2161 caatacaagt gggaaacgaa cgcgtttttt ccgggcccga agcggcaccc gcggaatcgc
     2221 gagtcgttcg atccacgatg aacctgcgct tatttcctgt tcaccgcgag gaaaccttca
     2281 tggctatctt tccagcaaca cctgcggcgc cggtcgccat tttgctgctg gccatggcgg
     2341 tgggcggctg cgccgccagg aacgagccgt tgccaaccgt gccttacgta gatctggaga
     2401 agttcatggg caactggtac gtgattgcca gcatcccgac gttcatcgaa aaggacgcct
     2461 acaacgcggt ggagacgtac gaactgcgca aagacggggc gatcgccacg actttcgtgt
     2521 tccgccaggg cggattcggc ggcaaggaaa aggcttatac gcctgtcggt tacgtagagg
     2581 acaaggaaac caacgcggtg tggggcatgc agttcatatg gccgatacgt gcagactatc
     2641 gcatcgtcta cctcgcagaa gattacagcc gcacgatcat tgcccgcaac aagcgggatt
     2701 tcgtctggat catggcccgc acgccggcga tcagcgccgg ggactacgag caaatgacgc
     2761 ggcttatcga agcaatgggc tacgacctgg ccaagctgcg cccggtaccg cagcgatggc
     2821 aggccgacca atgaaacttg ccccgggagc tttcgcatga ggatcgcgat cgtcggtacg
     2881 ggcatcgccg gaaacgtggt agcacatcgc ctgcaccctg ctcatgacat caccgtgttc
     2941 gagtcagcca gccgtatcgg cgggcacgcg aacaccatcg aagtagtatc gggcgacgca
     3001 acgctgaagc tcgacaccgg cttcatggtg ttcaacgagc gcacctaccc aaaattttgc
     3061 gcgctcctca aggagctgga ggtgggctcg caggacactg cgatgagctt tagtgtgcgg
     3121 cgggaaacga gcgggctgga gtactgcggc tcctccctca atgcgctgtt tgcacagcgc
     3181 gcgaacctcc tgcggcgatc ctttcaccgc atgctgcgcg acatagtgcg gttcaaccgc
     3241 gcggcaagcg aaggactcga cgacatagcc gagtcgacca cgttgggaga ataccttgaa
     3301 gccggccagt actcccgcga gttcgcacgc gactacctcg tgccgatggg tgcggcgatc
     3361 tggtcgaacc cgccggacac gctgcccgcc atgcccgcca ggtttttcgt ccgattcttc
     3421 cacaaccacg gcttgctcac tttccgcgac cgcccgcagt ggcgtgtgat caggggcggc
     3481 gccgagactt acgtgtccaa gctggtcgca gggcatcagc accggatccg tttgaacagc
     3541 ccggtcaatt cgattgccag gcggggtgga cgggtcgtgg tgagatcctg cggacggagc
     3601 gagagtttcg accggatttt cattgcctgt cacagtgacc aggcattgcg cctgctggag
     3661 gacgcgagtg gggccgagcg cgaagtc
//
LOCUS       DASUOM010000229         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_599373,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000229 DASUOM010000000
VERSION     DASUOM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599373"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFY27_04755"
     CDS             complement(<1..381)
                     /locus_tag="VFY27_04755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5764855.1"
                     /translation="MKADSSLRLRAAHRSEAAAMAALSRLHVEHGLRWRWTPPRVRQQ
                     IEDPDTMALVASVEGQFAGFAIMHFGDLRAHLVLLAVEPRFRKSGIGSALLRWLEASC
                     VTAGIQDIRLEVRAGNQLAQKFYRN"
     gene            560..1414
                     /locus_tag="VFY27_04760"
     CDS             560..1414
                     /locus_tag="VFY27_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5764856.1"
                     /translation="MSSKYEALLATAIEDQPVSYADSDAMLYALGVGFCSSPDNVKEL
                     PYAYESPALKTVPTFASMLPPPDFLADSGLQVARILLGELRLELYRTLPAAADLLLNR
                     RILGVYDKGMAHGAVIVVQSEARLKKDDTALFTLNSTLIARGDGGIGGSKGTPPEWHM
                     LPGRDPDLSCSSTTRPDQALLFRLSGDRNPLHVDVAAAREAGFTRPLLHGRCTYGIAC
                     RAILSTICDYDHTLITGFDARFSAPVYPGDTLTTDMWQDRNIVSFRCSVKARNSIVLS
                     NGKCTLAA"
     gene            1432..2241
                     /locus_tag="VFY27_04765"
     CDS             1432..2241
                     /locus_tag="VFY27_04765"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5764857.1"
                     /translation="MSKPMDDRVDLQIENHVALVRINRPEKHNALDREAFEALVEAGK
                     ALGRNRSVRAVVLAGAGESFCAGIDMSVFEGVGIADARRELMQPCGETGNLYQSAATV
                     WRELPVPVIAAVHGVAYGAGLQIAMGADIRIGAPSARFSIMEIRWGIIPDMGITVTMR
                     HVVPADRIRLLAYTGNVVDGTEALKLNLVTGLDDDPLAAAMHLAAAIAGRSPDAVRAI
                     KSLLNASLDEPQAEALRHEAQRQLSLLGSANNREAVMANLHKREPQFGDPQ"
BASE COUNT          486 a          727 c          759 g          438 t
ORIGIN      
        1 gttgcggtaa aacttctgtg ccagctggtt gcccgcccgt acttccagcc ggatgtcctg
       61 tatgcctgcc gtgacgcagg aagcctccag ccacctcagg agggcgctgc cgatcccgga
      121 tttccggaaa cgcggctcta cggccagcag gaccaggtgc gcgcgcaggt cgccgaagtg
      181 catgatcgcg aacccggcga actggccttc caccgaagcg accagtgcca tggtatcggg
      241 atcctcgatc tgctggcgta cgcgcggcgg ggtccagcgc cagcgaagcc cgtgttcgac
      301 gtgcagccgg gaaagcgccg ccatcgcggc cgcctcggaa cggtgcgccg ccctgaggcg
      361 caaagaactg tctgctttca tacctgccac ggtgatacaa ggatttatca atcgtaagat
      421 aacttgccgg gcccagacca ccccaataga atgcccgccc catagagtcc agcaggattg
      481 gacatatcgc ccgaggagcc gccttcagcc ggcggtcgcc acggcgccag gaagcccgtg
      541 cggcgggcgt gcgtataaaa tgtcatcgaa atacgaagct ttactggcga cggcaatcga
      601 agaccagccg gtcagctacg ccgacagcga cgcgatgctg tatgcgctcg gcgtcggttt
      661 ttgcagttct cctgacaacg tcaaggaatt gccctacgca tacgaatcac ccgcgctcaa
      721 gacggtgccg acgttcgcca gcatgctgcc gccgccggat ttcctggcgg acagcggcct
      781 tcaggttgcc aggatcctgc tcggcgaact tcgtctcgag ctgtaccgga ccctgcctgc
      841 tgcggcagac cttttgctca acaggcgaat actcggggtg tacgataaag gcatggcgca
      901 cggtgccgtg atcgtcgtgc aatccgaagc gcggctgaaa aaagacgaca cggcgctctt
      961 tactctcaac agcactctga tcgccagggg cgatggcggc ataggcggct cgaagggaac
     1021 gccgccggaa tggcatatgc tgccgggacg ggatccggac ctgagctgct cgagtaccac
     1081 gcgcccggac caggcgctcc tgtttcggct gtcgggggat cggaatcctc tgcacgtcga
     1141 tgtggcggca gcgcgcgagg cagggttcac tcggcctctg ttgcatggcc gttgtaccta
     1201 cggcatcgca tgccgggcga ttctcagcac catctgcgat tacgaccaca cgctcataac
     1261 cggcttcgat gcacgctttt ccgcgccggt ctatccggga gacacactta ccacggatat
     1321 gtggcaggac cggaacatcg tatcctttcg ttgctcggta aaggcacgca acagcatcgt
     1381 cctgagcaac ggaaagtgta cgctggcagc gtaagtgcag gcttaagtgg agtgagcaag
     1441 ccaatggacg atcgcgtcga cctgcagatc gaaaatcatg tggcgctggt gcggatcaac
     1501 cggccggaga agcacaacgc gctcgatcgc gaagcattcg aagcgctcgt tgaagcgggt
     1561 aaagcgttag ggcggaacag gtctgtacgc gccgttgttc ttgccggcgc cggcgaaagc
     1621 ttttgcgccg ggatagacat gtcggtcttc gaaggcgtgg gcatcgccga tgccaggcgt
     1681 gaactgatgc agccttgcgg agagaccggc aacctctacc agagcgcggc gactgtctgg
     1741 cgggagttgc cggtccccgt gattgccgcc gtccacggcg tcgcgtacgg ggcgggcctg
     1801 cagattgcca tgggtgcgga catacgcatc ggagcgccgt cggccaggtt ttccatcatg
     1861 gaaattcgct ggggcatcat ccccgacatg ggtatcaccg tgaccatgcg tcacgtagtg
     1921 ccggcagaca ggatcaggtt gctggcctac accggcaacg tcgttgacgg caccgaggcg
     1981 ctgaagctga acctggtcac tgggcttgac gacgatccgc tcgcagcggc gatgcacctt
     2041 gctgccgcga tagcagggcg atcacccgac gcggtcaggg cgatcaagtc actcctgaac
     2101 gcatcgctgg atgaacctca ggcggaagcg ctgcggcacg aagcacaacg gcagctttcg
     2161 ctgctgggtt ccgccaacaa ccgggaggcg gtaatggcga atttgcataa gcgcgagccg
     2221 caattcgggg acccgcagta agccagggaa atttcaagct cacgtttgcc ggatcaagag
     2281 gaactccggg cagcgggaaa cagtctcaga ggatatattc cctgatgggc atcggaccgc
     2341 gctggcgggt gaacgactcc cggcatgagg cttgcttgaa caaattcttt ttgccgatta
     2401 atttcacatc
//
LOCUS       DASUOM010000230         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_319428,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000230 DASUOM010000000
VERSION     DASUOM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319428"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFY27_04770"
     CDS             <1..360
                     /locus_tag="VFY27_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5764858.1"
                     /translation="GRLPAVNQIEWTPFGCSDDMLQYCRENGIVIQAYSPLTRGKRLD
                     DETLAEMSGRYGRSPAQLLIRWNLERGTVPLPKANQARHLEENLDVFDFELSEDDMLT
                     LNGLNDRYSSLGTLPYA"
     gene            complement(472..729)
                     /locus_tag="VFY27_04775"
     CDS             complement(472..729)
                     /locus_tag="VFY27_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor"
                     /protein_id="HEX5764859.1"
                     /translation="MAQKNHGSSIKDDEQYEALRRKGMSKEKAARIANTDRKTAAKRG
                     GKSAKYEEWSKEDLYEKAREVGIGGRSNMSKDQLIDSLRNH"
     gene            complement(783..1073)
                     /locus_tag="VFY27_04780"
     CDS             complement(783..1073)
                     /locus_tag="VFY27_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764860.1"
                     /translation="MAKASESKRTDDHETIRKWAEERDARPSRVKSTASKDDTGILRM
                     DFPGYSGADELEEISWDEFFDKFDQENLEFLYQEKTEKGEISRFNKFVNRQT"
     gene            1373..>2233
                     /locus_tag="VFY27_04785"
     CDS             1373..>2233
                     /locus_tag="VFY27_04785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5764861.1"
                     /translation="MSGRQRALSESARIQAEEALRASEERYRILVESQSEMLCRFRRD
                     GMILFVNGAYARARGTTAEALTGSNFWSFVPQAERPAVEAMLDRLTPESPEIRIENLF
                     QTVDGERWILWTNRGLIFDADGRLLEAQSAGIDITRRKEMERALKDSDQRKDEFLATL
                     AHELRNPLAPIGNALEILKQAGDDPEILTKARGAIERQLSHLVRLVDDLLDISRITRD
                     KLELRKTRADLNSAIRHAVETCLPLAEAKRQTIALDLPEEIIHLDADPVRLAQIFNNL
                     LNNASKYTERG"
BASE COUNT          434 a          700 c          634 g          465 t
ORIGIN      
        1 ggtcggctgc cggccgtcaa ccagatcgag tggacgccgt tcggctgcag cgatgacatg
       61 ctgcaatact gcagggaaaa cggaatcgtc atccaggcat atagtcccct cacgaggggc
      121 aagcggctgg acgacgagac actcgcggag atgtccggac gctatggccg atcgcccgcc
      181 cagttattga ttcgctggaa tctcgagcgg ggtacggtcc cgttgccgaa ggcaaaccag
      241 gctcggcacc tcgaggagaa tctcgacgta ttcgacttcg aactgagcga ggatgacatg
      301 ctcacgctca acggcctcaa cgatcgctat tcttccctgg gcactctgcc ctacgcgtga
      361 cacctcgtct cgtcgataca gtcgtggcta cggctttgcc cgcagtcccc gggtttgatg
      421 tgccccttcc tccaggagtc aggctacctc ccgcattccg cggctgaagt ttcagtggtt
      481 acgcaaggag tcgatgagct ggtccttgct catgttggag cgaccgccga tgcccacttc
      541 cctggccttc tcgtacaggt cttccttgct ccattcctcg tactttgcgg acttgccgcc
      601 gcgcttcgcc gccgtcttcc ggtcggtatt cgcgatgcgt gccgcctttt ccttgctcat
      661 tcccttgcgg cgaagagctt catactgctc gtcgtccttg atgctcgaac cgtggttttt
      721 ctgcgccatg ctggtcctcc tccgggatta cgccggcgtc atctacgcca acgtcgtcac
      781 gattacgttt gcctgtttac gaacttgttg aagcggctga tctctccctt ctctgtcttt
      841 tcctggtaca ggaactcgag gttttcctgg tcgaacttgt cgaaaaactc gtcccaggag
      901 atctcttcca gttcgtcagc gccgctgtag cccggaaaat ccatgcgcag gatcccggtg
      961 tcgtctttcg aagcagtact cttcacgcgc gatggcctcg cgtcgcgttc ctccgcccac
     1021 ttccgaatgg tttcatgatc gtccgtccgc ttgctctcgc ttgctttcgc catgtctctc
     1081 cctccgggtg ctctgcaatg gttgggctgc tccaatgtcc gaagaatcag ctgcaaattt
     1141 tgtgccacgg gtggtcgcag gcgaaggcag gacctcctgt acctcctccg gccgctccga
     1201 cacaaagcga agtgcgcctg aagcagttag aatgactaac gcccctgtgg gccagctttc
     1261 gacaggtcac cagcgaccgt tgaccatcag cgagaagaaa taatcaacaa gaaatggctc
     1321 ccgggaaaag tacacggcac cgtccggcag tggtgataac ggcgagtgcc gcatgagcgg
     1381 ccgacagcgc gccctcagtg aatccgcaag aatccaggca gaggaggcgt tgcgcgcaag
     1441 cgaggaacgt taccgtattc tcgtcgaaag ccagtcggag atgctgtgcc gtttccggag
     1501 ggacggaatg attcttttcg tcaatggcgc ctatgcccgc gcccggggca cgacggcgga
     1561 agcgctcact ggcagcaact tctggtcgtt cgtgccgcag gcggaacggc cggcggtgga
     1621 ggccatgctc gaccggctga cgcccgagtc gccggagatt cgtatcgaga acctcttcca
     1681 gacggtcgac ggcgagcgct ggatcctgtg gacgaatcgc ggcctgatct tcgacgcgga
     1741 cgggcgcttg ctggaggcac aatccgcagg catcgacatt acccggcgaa aagagatgga
     1801 acgggcgctc aaggattccg accagcgcaa ggacgaattc ctggccacac tggctcatga
     1861 gcttcgcaat ccgcttgcgc caatcggtaa cgcgctcgaa atactcaagc aagcgggcga
     1921 cgatccagag atcctgacaa aagcccgggg ggcaatagag cgccagctgt ctcacctggt
     1981 gcggctcgtg gacgacctcc tggacatcag ccggattacg cgcgacaagc tggaactgcg
     2041 caaaacccgt gcggatctca actcggccat tcgccacgcg gttgaaacct gcctgccgct
     2101 tgcagaagca aaacggcaga cgatcgcact cgatcttccc gaggagatca ttcatctcga
     2161 cgccgatccg gtgcgcctgg cccagatctt caacaacctc ctgaacaacg cctccaagta
     2221 caccgaaagg gga
//
LOCUS       DASUOM010000231         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_581016,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000231 DASUOM010000000
VERSION     DASUOM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581016"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFY27_04790"
     CDS             <1..304
                     /locus_tag="VFY27_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5764862.1"
                     /translation="HRSEEFWPEPDRFDPGRFIADRGMDKGEGKKGERPYFPFSLGPR
                     RCLGEYFSFLEMKIHLGLLARKFRMSCTEAAVPPLDLGINLRSATDIYLRPSFRTS"
     gene            307..2250
                     /locus_tag="VFY27_04795"
     CDS             307..2250
                     /locus_tag="VFY27_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX5764863.1"
                     /translation="MQLYDSLTAILRDAGTKDRCIRFIDGEQDERVLKFADLWQRGVL
                     VLGALQSRGMKPGDELVIFTRSNETFVVAFWAALLGGIVPVPVAVGISDEHRLKLFRI
                     VTQLQHATLLTDRDLFERLTDFAASHELRDVQAVLRDRVMLDEEMISGTAGEVLDAEP
                     DGVAFIQYSSGSTSDPKGVCLTHRNLTTNIRAIVEGARWTDQDQSLSWMPLTHDMGLI
                     GFHLSVLAAHMNHAVMDTSLFVRRPLYWMTRASTLGATQLCSPNFGFKHFLNMFLRKG
                     ADGLDLSAVRLIMNGAEPISYDLCEQFLGALQPFGLKRTAMFPVYGLAEATVAVSFPQ
                     IGVEYSRIVVDRHSLRIGARYETAEPGDEQAVSFVKVGSAVRDCLIRITDDDDQVLSP
                     MVVGNIQVKGSNVTGGIYNDAQAEKALFTGDGWLRTGDVGVFVDGELVITGRSKDIII
                     VNGQNYYPHDIEEVIAAIDGLELGKVVACGTSRAGSPDEELLVFVLYRQDLEAFPPLA
                     HSVRTRVGTNTGLEVDHVIPVARIPKTTSGKIQRSQLAAAYLDGEFASVLAAIEPSSG
                     EDPADSPGGEDPLILELEQICREFAKDSVIGPDDNLFEVGVSSLTLTEIVLAIDEKHP
                     GKVDISDLFDYPTIRQLAAFLQR"
     gene            2437..>2737
                     /locus_tag="VFY27_04800"
     CDS             2437..>2737
                     /locus_tag="VFY27_04800"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258182.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX5764864.1"
                     /translation="MIYAKIAGTGSYLPERVMHNREFEAFVDTSDQWIRERTGIARRH
                     IAADNETTSDMSVAASRHAMEQAGVTARDIDLIIVATTTPDKVFPSTACIVQRKLD"
BASE COUNT          566 a          809 c          833 g          529 t
ORIGIN      
        1 ccatcgcagc gaggaattct ggccggagcc ggatcgtttc gatcccggcc gcttcattgc
       61 cgaccggggg atggacaagg gtgaagggaa gaagggcgaa cggccatatt tcccgttctc
      121 gctggggcca cggcgctgtc tcggcgaata tttttcgttc cttgaaatga agattcacct
      181 ggggctgctt gcccggaagt tccgcatgag ttgtaccgag gcggcagttc ctccgctgga
      241 cctcggcatc aacctgcgtt ccgccaccga tatatacctt cgaccgagct ttcgcacaag
      301 ctgacaatgc agttatacga cagccttacc gccatcctgc gcgacgcagg aaccaaagac
      361 cgttgcatcc ggttcatcga cggcgagcag gacgagagag tccttaagtt tgcggacctg
      421 tggcaacggg gtgtgctcgt gctcggcgct ttgcagtcac gtggcatgaa gcccggcgac
      481 gagctggtta tcttcacccg gtccaacgaa acattcgtag ttgcgttctg ggcagcgctt
      541 ctgggcggca tcgtgccggt tcccgttgca gttggcatca gcgacgagca caggctgaaa
      601 ctctttcgca tcgtgactca gctgcagcac gccacgttgc tgaccgacag agacctgttc
      661 gaacgcctca ccgattttgc cgccagccat gaactccgtg acgtgcaggc agtgcttcgc
      721 gaccgggtga tgctggatga ggagatgata tccggaacgg cgggcgaggt cctcgatgca
      781 gagcccgatg gtgtggcatt catccagtac tcgtcaggct cgaccagcga tccgaagggt
      841 gtgtgcctga cccaccgcaa cctcacgacg aatatacgcg ccatcgtgga aggcgcgcgc
      901 tggacggacc aggatcaaag cctgagctgg atgcccttga cccacgacat gggcctgatc
      961 ggcttccacc tgagcgtgct cgccgcccac atgaatcatg ccgtgatgga cacaagcctg
     1021 tttgtgcggc gtccgctcta ctggatgaca cgggcgagca cgctcggagc aacgcagctc
     1081 tgctcgccga acttcggctt caagcacttc ctcaacatgt tcctgcgcaa aggtgctgac
     1141 gggctcgacc tttcggcggt gcgcctgatc atgaacgggg cggaaccgat ctcctatgac
     1201 ctgtgcgagc agttcctcgg cgcgctgcag ccattcggtt tgaagaggac cgcgatgttc
     1261 ccggtttatg ggttggccga agccaccgtc gccgtaagct tcccgcagat cggcgtcgag
     1321 tactcacgga tcgtggtaga ccggcacagc ctgcgcatcg gcgcgcggta tgagactgcc
     1381 gaaccgggcg atgagcaggc ggtcagcttc gtcaaggtgg gaagcgcggt gcgcgattgc
     1441 ctgatccgca tcaccgatga cgacgaccag gtgctgtcgc ccatggtggt cgggaacatc
     1501 caggtgaaag gttctaacgt caccggcggg atttacaatg atgcgcaagc ggagaaggca
     1561 ctgttcacgg gcgacggctg gctgcgtacc ggcgacgtcg gcgtattcgt cgatggtgaa
     1621 cttgtcataa cagggcggtc gaaggacatc atcatcgtca acggccagaa ctattatcca
     1681 cacgacatag aggaagtgat tgcagccatc gacggcctcg aattgggcaa ggtcgtggcg
     1741 tgcggaacga gccgcgccgg cagccccgat gaggaactgc tggtgtttgt gctgtatcgt
     1801 caggacctcg aagccttccc gccactggcg cacagcgtcc gcacgagagt ggggaccaac
     1861 acgggactcg aggtcgacca cgtaatcccg gttgcgcgaa tcccgaagac gacgagcggc
     1921 aagatacagc gcagccaatt ggcagcggct tacctcgatg gagagttcgc ctcggttctt
     1981 gctgcgatcg agccctcttc cggcgaggac ccggcagatt cgcccggggg ggaagacccg
     2041 ctgatactgg aactcgagca gatctgccgc gagtttgcga aggatagcgt gataggtcct
     2101 gacgacaacc tgttcgaggt cggtgtgagt tcgctgacgc tgacggagat cgttcttgca
     2161 atagacgaaa aacacccggg caaggtcgac atcagcgacc tgttcgacta cccgacgata
     2221 cggcagctgg ccgcattttt gcagcgctga ccgcgctgtg acaggaactt agcccggact
     2281 attcatgaat acactcgcgc ccttgcttga tccttgttcg cacaagaaat tttcctccct
     2341 cgggaataca ctcaggtatt ccgctctgtc ggaaaacccc ttgtgcgaac aagtctctgc
     2401 ccaatcatcg cggtgattca tgaatagtcc gagctaatga tctacgcaaa aatcgcaggc
     2461 accggcagct acctgcccga gagggtaatg cacaaccggg agttcgaggc attcgtcgat
     2521 acctcggacc agtggatacg cgagcgcacc ggcatcgcac gccgccatat cgcggcagac
     2581 aacgagacga cgtccgacat gagcgtggct gcgtcccgtc atgcgatgga gcaggcaggc
     2641 gtcactgccc gcgacatcga cctgatcatc gttgccacga cgacaccgga caaggtgttt
     2701 cccagtaccg cgtgcatcgt gcagcgaaag ctcgaca
//
LOCUS       DASUOM010000232         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1507285,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000232 DASUOM010000000
VERSION     DASUOM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507285"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1997
                     /gene="parE"
                     /locus_tag="VFY27_04805"
     CDS             114..1997
                     /gene="parE"
                     /locus_tag="VFY27_04805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5764865.1"
                     /translation="MSRRYDAADIEVLSGLEPVRRRPGMYTDTTRPNHLAHEVVDNSV
                     DEALAGHCKKIEVTLHEDGSVEVSDDGRGMPVDIHPKEKIPGVELILTRLYAGGKFNN
                     ENYQFSGGLHGVGVSVVNALSKNLEVWIRRGGKEHNMSFAGGNKRGKLEVVGTVGKQN
                     TGTTIRFWPDPRYFDSAKISVSRLKHALRAKAVLCPGLLVRLKVARPHEEVEWQYTGG
                     LEEYLLEAVGNVEHLPADPFSGSLSGKHEGVEWALVWTAEAGQVGESYVNLIPTPQDG
                     THVNGMRTGLTTALREYCEFRNLLPRGVQLSPEDVWEGLNFVLSAKLENPQFSGQTKE
                     RLSSRESAAFVGGVIKDSFSLWLNQHPESGDQIAELAISKAQSRLKAAKKVVRKKIVS
                     GPALPGKLADCRSQEPELCEIFLVEGDSAGGSAKQARDRNTQAILPLRGKILNTWEVD
                     TGEILASQEVHDISVALGVDPGSNDIDGLRYHKICILADADSDGAHIATLLCALFLRH
                     FRNLVLAGHVYIAMPPLFRIDVGKEVFYALDRDERKGILDRIEAEKIRGKVSVTRFKG
                     LGEMSPQQLRETTMHRDTRRLVRLTIDAKDNTDQLMDMLLAKKRAPDRRTWLETKGNL
                     AEV"
BASE COUNT          463 a          666 c          671 g          355 t
ORIGIN      
        1 gggagagacc ccaactcgaa actgcagatc atctctaaat tccatcaccc ccgcgctttc
       61 tgtgatcctg ctagaatcgc ccgcgtgaat ttcgcttgtg gcgccaaaac ccgatgagta
      121 gacgatacga cgcggccgat atcgaggtcc tgagcggtct cgagcccgtg cgccggcggc
      181 ccggcatgta cacggacacg acgcggccga accaccttgc gcacgaggtc gtcgataaca
      241 gcgtcgatga agcactcgcc ggacattgta agaagatcga agtcacgcta catgaagacg
      301 gttcggtgga agtgagcgac gacggccgag gcatgccggt cgacatccac ccgaaggaga
      361 aaattcccgg cgtcgaactg atcctgaccc gcctgtatgc gggcggcaag ttcaacaacg
      421 agaactacca gttttccggc ggcttgcacg gcgtcggcgt gtccgttgtc aacgcgctgt
      481 cgaaaaacct cgaagtgtgg atccgccgcg gcggcaagga acacaacatg agcttcgccg
      541 gcggcaacaa gcgcggcaag ctcgaggtcg tcggcacagt tggcaaacag aacaccggca
      601 ctacaatccg cttctggccg gacccgcggt atttcgattc ggcgaagatc tcggtatcgc
      661 ggctcaagca cgccctgcgg gcgaaagccg tgctttgccc gggactgctg gtaaggctga
      721 aggttgcaag gccccacgaa gaggtggagt ggcagtacac cggcgggctg gaggagtacc
      781 tgctggaagc ggtcggcaac gtggagcatc tgccggcaga ccctttctca ggcagccttt
      841 ccggcaagca tgagggcgtg gaatgggccc tcgtctggac ggcggaggca ggccaggtcg
      901 gcgaaagcta cgtcaacctg atcccgacgc cgcaggacgg cacgcacgta aacggtatgc
      961 gcaccgggct gacgacggcg ctgcgcgaat actgcgagtt ccgcaacctg ttgccgcgcg
     1021 gtgtgcagct ttcgcccgaa gacgtgtggg aaggcctcaa cttcgtcctc agcgcgaaac
     1081 tcgagaaccc gcagttctcc ggccagacaa aggagagact ttcctcgcgc gaatcggccg
     1141 cgttcgtcgg cggcgtcatc aaggacagtt tctccctttg gctcaaccag cacccggagt
     1201 ccggcgacca gatcgcggag ctcgcaattt ccaaagcgca gtcgcggctg aaggccgcga
     1261 agaaggttgt gcggaagaag atcgtctcgg gccccgcgct gccgggaaaa ctcgcagact
     1321 gccgctcgca ggagccggag ctatgcgaga tcttccttgt agagggcgac tcggcaggcg
     1381 gctccgccaa gcaggcccgc gaccgaaaca cgcaggctat cctgccgctt cgcggaaaaa
     1441 tcctgaatac ctgggaagtc gataccggcg aaattctcgc ctcgcaggaa gtgcacgata
     1501 tcagcgtcgc gctcggcgtg gatcctggca gcaacgacat cgatggattg cgctatcaca
     1561 agatctgcat cctcgccgat gccgactccg acggcgcgca catcgcgacc ctgctttgcg
     1621 cgctgttcct tcgacacttc cgcaacctcg tcctcgccgg tcacgtctac atagcgatgc
     1681 cgccgctctt tcgcatcgac gttggcaagg aagtcttcta cgcgctcgac cgcgacgaac
     1741 ggaagggcat tctcgaccgg atcgaggcgg aaaaaattcg tggcaaggtc agcgtcaccc
     1801 gcttcaaggg actgggcgag atgagcccgc agcagcttcg cgaaacgacg atgcaccgcg
     1861 acacccgccg cctcgtcagg ctcacgatcg acgcaaaaga caacaccgac cagctcatgg
     1921 acatgctgct ggccaagaag cgcgctcccg acaggcgcac ctggctggag accaagggta
     1981 accttgccga ggtatagatg gtgatgttcg cgacgccgcg acagacagca gcacgcacgg
     2041 cgcatgaacg cgaaacgctt cagcggagcc ttggttgggg ggagggcttg tcaggactgt
     2101 cgcaaacagg gatgtttgcg cagagccccc atggatgggt tcacggcgtt cctga
//
LOCUS       DASUOM010000233         6262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_500294,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000233 DASUOM010000000
VERSION     DASUOM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500294"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2335
                     /locus_tag="VFY27_04810"
     CDS             137..2335
                     /locus_tag="VFY27_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764866.1"
                     /translation="MQTPIQARRSLVTVAALAGLGISAGGQAQQRASEVFLEEITVTA
                     TKRAGGIDVQDAALAVSAYGENQIDAMHLRDLSSIGFVAPSVQLEDIGTTRGTANFTI
                     RGIGINSSIPSIDPAVGVFVDGMYLGINGGVVLDVFDLEGIEVLRGPQGLLFGRNVTG
                     GAVLLRTTRPTDEFHVNARVAAETGLNQYYSAVVSGPISDAVGGKVAVYHNSDDGWHE
                     NLATGEDHGAADTTIFRAAFDFAPGDDFDAILRYDHGESDGDGPAAQNAGLFSPESFD
                     FSIDEPGNYDASWDQAILEMTWDVGLGDGQVVNILGYREYQNDTLGDIDSSPIHVFHA
                     LSTTDQSQWSNELRYSGSFNNWYVTSGIFFLEQDLGYFEQRLIPPAGLDITGGGYQDM
                     RSVGVFTQADVVITDEVTLNFGARYDREKKEAQISGLFRNQCVLGGGCASYDLDDSQT
                     WNAFTPKIGIQLSPTESTMMYAFWTKAFRSGGYNMRHTAILIPNAAFDQEEMNSVEVG
                     LKSDFADGRVRVNAAVFHNQFDDMQREINLTDPVTGVVQLIRNTADTTITGADAEIGW
                     AVTDSLFLQANIGYVDGTYDEVKFDLTGDNVIDAADRELEIPRLAPWSYGAQFTYQRP
                     WSQGTFTVQGSGYHRDASAYTDNNRGWLRPSDMFNANISLGFMDDRLTVSAFGKNLKD
                     EVTIGGDTQLPPNFPGGPAFPVPSLAGPQATFSPLNKGRIYGLEVQYRME"
     gene            complement(2451..3470)
                     /locus_tag="VFY27_04815"
     CDS             complement(2451..3470)
                     /locus_tag="VFY27_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5764867.1"
                     /translation="MSKGTNVRIRLASRPRGWVTAQNFEQTESPVPEPAEGQVLVRNI
                     YLSVDPYMRGRMNDAKSYVPPFGLNEVLQGGVVGQVVKSRNAGFAEGDYVNGMLGWEN
                     YSLSNGQGLYKISKQEVPLSYHLGILGMPGMTAWVGLFTIAGTKPGETVFVSAASGAV
                     GSIVGQIARIHGCTVAGTAGSDEKVELLKKEFGYHAAFNYRASESLPKSIASVCPKGI
                     DVDFENVGGAIFEAALWNMRAYGRIALCGMVSNYNDDKSEPGPRGMMSIVGKRLTIRG
                     FIVADHPDACREYVSKASVWLKEGKLKYRETVTQGIENAPAAFIDMLKGGNIGKQIVQ
                     LDEEL"
     gene            complement(3528..4190)
                     /locus_tag="VFY27_04820"
     CDS             complement(3528..4190)
                     /locus_tag="VFY27_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5764868.1"
                     /translation="MTDTYPISPRNKVRQLKEKARYEKDTVHQLLDSGLVAHVGFVQD
                     GAPVVVPMIYGREDEIVYLHGARKARVIRLLEQTECICLNVTMLDAIVLARSAFNSSM
                     NYRSVTVFGKPRLLETDDEKLHAMRVISEHTMPGRWDELRAPHDREVKMTGVIAVDVE
                     TGSAKISAKMPDDDPEDYDIPVWAGILPLTTRTGALQEDSRLVEGVEPSGIVLAMQHR
                     MI"
     gene            complement(4206..5669)
                     /locus_tag="VFY27_04825"
     CDS             complement(4206..5669)
                     /locus_tag="VFY27_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5764869.1"
                     /translation="MSEQDDKHQRATHQRVGLWLGPAVAAAVVLADSPESLNQAGWNT
                     AAMGLLMAIWWATEAVPIAVTALLPLVFFPLLGIADMDQAAAPFANKVIYLFLGGFVI
                     ALAMQRWNLHHRIALSVLQHAGRNGRTLVGGFMVACALLSMWVSNTSTTLMMLPIALS
                     VIEVMDRTVHGQSEKARGDFRLALLLGVAYAASIGGMATLVGSPPNAMMAAFLQESYA
                     IEVDFAHWLLVGLPLTLVLLPLTWLVLTRLLFRVDFTTSGEGRALLRQMKNDLGVISG
                     PEKRVAAIFLVVAITWMLRPLLARLPGLDLLDDSGIAMAGAVALFLVPSGDRGDPMLL
                     RWHHVEKLPWNILILFGGGLALAAAVTDTGLARWLGANLQAIASLPVPLLVLIVTSMI
                     VFLTELTSNTATAATFLPVVAAVAIEAGMDPLVLVVPVTLAASCAFMLPVATPPNAIV
                     FASGALTIPQMAKAGLVLNLIAIALITLLALLIAPLL"
BASE COUNT         1210 a         1948 c         1883 g         1221 t
ORIGIN      
        1 gattgttaca ttatccaagc agtttcagta ccatacgccg catactcaaa aacccttgac
       61 ggcgtcgcgc gctcaccgcc cgtttgttgt ggtcgccaca atgccgtcat cataagaagc
      121 tgacggagat ctgatgatgc aaactccaat tcaggcgaga cgcagcctgg taaccgttgc
      181 ggcgcttgcc ggactcggaa tatccgcggg gggacaggcg caacagcgtg cgtcggaagt
      241 gtttctcgag gagattacgg tcaccgcgac caagcgcgcc ggcggcatcg acgtacagga
      301 cgcggcgctg gcggtgagcg cctacggtga gaaccagatc gatgccatgc atctgcgcga
      361 tctttcttcc atcggtttcg tcgcgccgag cgtgcagctc gaagatatcg gtaccacacg
      421 aggcacggcg aatttcacca ttcgcggcat cggcatcaac agctccattc cctcgatcga
      481 tcccgcagtg ggcgtattcg tcgacggcat gtatctcggg ataaacggcg gtgtggtcct
      541 cgacgtgttc gacctcgagg gcatcgaggt gctgcgcggg ccgcaaggcc tgctctttgg
      601 caggaacgtc accggcggtg cggtcctgtt gcgtacgacg cggccgactg acgagttcca
      661 cgtcaatgct cgggtggcgg cggaaaccgg cctcaaccag tactactcgg cggtggtttc
      721 gggaccgatc agcgatgctg tcggcggcaa ggtagcggtg tatcacaact cggacgacgg
      781 ctggcatgag aacctcgcga cgggcgagga ccacggcgca gccgatacga ccatcttccg
      841 cgccgcgttc gatttcgcgc ccggcgatga tttcgatgcc atcctgcgtt acgaccatgg
      901 cgagtccgac ggtgatggtc cggcagcgca gaacgccggt ctcttcagcc ccgaaagttt
      961 cgatttctcg atcgacgaac ccggtaacta cgatgcgagc tgggaccagg cgatcctcga
     1021 gatgacgtgg gatgtgggtc tcggtgacgg gcaggtcgtc aacattcttg gctatcgcga
     1081 ataccagaac gacacgttgg gcgatatcga ttcttccccc attcatgtct tccatgcatt
     1141 gtcgacgacc gaccagagcc agtggtcgaa cgagctgcgc tattccggtt ctttcaacaa
     1201 ctggtacgtg acgagcggaa tattcttcct cgagcaggat ctcggctact tcgaacaacg
     1261 cctgattccg ccggcgggac tggatatcac gggcggcggc taccaggaca tgcgttccgt
     1321 aggggtgttc acgcaggccg acgtggtcat aacggatgaa gtcacgctga actttggcgc
     1381 gcgctacgat cgcgagaaga aggaagcgca gatttcaggc ctgtttcgca accagtgcgt
     1441 ccttggcggc ggttgcgcgt catacgacct ggatgactcg cagacatgga acgcgttcac
     1501 gccgaagatc ggcatccagc tttcgccgac cgagtccacc atgatgtatg cgttctggac
     1561 caaggccttc cgtagcggcg gctacaacat gcgccatacc gccatcctga taccgaacgc
     1621 cgccttcgac caggaggaaa tgaacagcgt ggaggtcggc ctgaaatccg atttcgccga
     1681 tggacgggtg cgagtcaacg cggccgtgtt ccacaaccag ttcgacgaca tgcagcgcga
     1741 gatcaacctg acggatcccg tcacgggtgt cgtgcagctc attcgcaaca ccgcggacac
     1801 cacgatcacc ggcgctgatg cggagatcgg ctgggcggtt accgattcgc tgttcctgca
     1861 ggccaacata ggatacgtcg acgggaccta cgacgaggtg aagttcgacc tgaccggcga
     1921 caacgtcatc gacgccgccg atcgagagct cgagataccg cgcctggcgc cgtggtcgta
     1981 cggcgcgcag ttcacgtacc agcggccgtg gtcgcagggg acgttcactg tccagggtag
     2041 cggttaccat cgcgacgcca gtgcctacac cgataacaac cgcggctggc tgcgcccctc
     2101 cgatatgttc aacgcgaaca taagcttggg gttcatggac gatcgtctca cggtttccgc
     2161 cttcggcaag aacctgaagg atgaagtgac gatcggtggc gatacgcaat tgccgccgaa
     2221 cttccccggc ggcccggcgt ttcccgtgcc gagcctcgcg ggcccgcaag cgactttctc
     2281 gccgctcaac aaggggcgca tctacggcct ggaagtgcag taccgcatgg agtagcagtt
     2341 cacgcggccc ggatcgacga aacgccggca atcgccggcg tttttcttgt aggagcccgc
     2401 cgtgcgggcg agttcggtaa ggcttggcga gcccgccgcg tggcccgctc ctacaattcc
     2461 tcgtcaagct gcacgatctg cttgccgatg ttcccaccct tcagcatgtc gatgaacgcg
     2521 gccggtgcgt tttcgattcc ctgcgtgacg gtttcgcggt acttgagctt gccttcctta
     2581 agccacactg atgcctttga tacgtattcg cggcaggcgt cggggtggtc cgcgacgatg
     2641 aacccgcgga tcgtcagcct cttcccgaca atactcatca ttccgcgagg gcccggttcg
     2701 ctcttgtcgt cgttgtagtt ggagaccatg ccgcacagcg cgatgcgccc gtaagcccgc
     2761 atgttccaca gtgctgcttc gaagattgcg ccgccgacgt tttcgaagtc gacgtctatt
     2821 cctttcgggc aaacggatgc gatgctcttc ggcagcgatt cgcttgcacg gtagttaaag
     2881 gcagcgtggt agccgaattc ctttttcagc aactccacct tttcgtcgga gccggcggtg
     2941 ccggcaacgg tacatccatg gatcctggca atctggccga cgatgctgcc aacggccccc
     3001 gaggccgcgg acacgaatac cgtttcgccg ggtttggtgc cggcgatggt gaacaggcca
     3061 acccaggctg tcataccggg catgccgagg atgccgaggt ggtacgagag tggcacttcc
     3121 tgtttgctta tcttgtacag gccctgcccg ttcgacagcg agtagttctc ccagccgagc
     3181 atcccgttca cgtaatcgcc ttccgcgaaa cctgcgttcc tgctcttgac gacctggccg
     3241 accacaccac cctgcagcac ctcgttaagc ccgaacggcg gtacatagga cttcgcgtcg
     3301 ttcatgcgcc cgcgcatgta agggtcgacc gagaggtaga tgtttcgcac caggacctgg
     3361 ccttcggcgg gttccggcac cggcgattcc gtctgctcga agttctgcgc ggtcacccag
     3421 ccgcgtgggc gactcgccag cctgatccgg acgttcgtac ctttgctcac gggttgcgcc
     3481 tccttaaagg ccttactggt cttgaatagt ggctagatcg atagtggcta gatcattctg
     3541 tgctgcatcg ccagcacgat gcccgagggt tccactcctt cgaccagtcg gctgtcctcc
     3601 tgcagtgccc cggtacgggt agtgagcgga aggatgcccg cccagaccgg gatatcgtaa
     3661 tcctccggat cgtcgtccgg cattttcgcc gatatcttgg cggagcccgt ttccacgtcc
     3721 acggcgatta cccccgtcat cttgacctcc ctgtcgtgcg gcgccctcag ctcgtcccag
     3781 cgtcccggca tggtgtgctc gctgataacc ctcattgcgt gcagcttctc gtcgtcggtt
     3841 tcgagcagcc ggggcttgcc gaacaccgtc accgaccggt aattcatcga tgagttgaac
     3901 gcggagcgcg cgaggacgat cgcatccagc atggttacgt tgaggcagat acattccgtc
     3961 tgttccagca ggcgtataac gcgcgccttg cgggcgccgt gcaggtagac gatttcatcc
     4021 tcgcggccgt aaatcatcgg tacgacgacg ggtgcgccgt cctggacgaa accaacgtgt
     4081 gcgacaaggc ccgagtccag cagctgatgc accgtgtcct tttcgtaccg agccttttcc
     4141 ttgagctggc gtaccttgtt tcgcggggat atgggatatg tgtcagtcac ggtgtgtcat
     4201 cttccttata agagcggcgc gatcaacagc gcgagcagag tgattaaagc aattgcgatc
     4261 aggttcagga ccagtccggc cttggccatc tgcggaatgg tcagggcacc cgacgcgaat
     4321 acgatggcgt tcggcggggt cgcgaccggc agcatgaacg cacagctggc tgccaaggtt
     4381 accggcacga ccaggacgag cggatccatg ccggcttcga tcgcgactgc cgcgaccacc
     4441 ggcaggaacg tcgcggcggt cgccgtgttg ctcgtcagtt cggtgaggaa aacgatcatc
     4501 gatgtcacga tcagcacgag taacggcaca gggaggctcg caatcgcctg caggttggct
     4561 cccagccagc gggcaagacc tgtatcggtg accgccgccg cgagtgcgag cccgccaccg
     4621 aagagaataa ggatgttcca cgggagtttt tcgacgtggt gccagcgaag gagcatcggg
     4681 tctccccggt cgccgctcgg caccaggaac agcgccaccg cgccagccat tgcgatgccg
     4741 gaatcgtcca gcaggtcgag gcccggcagg cgcgccagca acggccgcaa catccaggtt
     4801 atcgcaacca ccaggaagat cgccgcaacc cgtttctccg ggccggagat aacgccaagg
     4861 tcgttcttca tttgcctgag caaagcacgg ccttcgccgg aggtcgtgaa gtcgacccga
     4921 aacagcaggc gcgtgagcac cagccacgtc agcggcaaca gcaccagtgt gagtggcagc
     4981 ccgaccagca gccagtgcgc gaaatccacc tcgattgcat agctctcctg caggaacgcc
     5041 gccatcatgg cattcggcgg ggatcccacc agcgtggcca tgccgccgat cgaagccgcg
     5101 taggctacgc cgagcaggag ggcgaggcga aaatcgcctc gcgctttctc gctctggccg
     5161 tgaaccgtgc gatccatcac ctcgatcacg gagagcgcaa tcggcagcat catcaatgtc
     5221 gtcgacgtgt tggaaaccca catgctgagc aaagcgcatg caaccatgaa tccgccgacc
     5281 agcgtccggc cgtttcgacc ggcgtgctgc aatacgctca aggcaatgcg gtggtgcagg
     5341 ttccagcgct gcatggccag ggcgatgacg aatccgccca ggaacaggta gatcaccttg
     5401 ttcgcgaagg gcgcggccgc ctggtccata tcggcgatac ccaacagcgg gaaaaacacg
     5461 agcggtagca gagccgtgac ggcgatgggt actgcttcgg ttgcccacca gatggccatg
     5521 agcaaaccca tggccgcggt gttccacccc gcctggttca gcgactccgg cgaatctgcg
     5581 aggacaacgg cggctgcaac ggctggtccc agccacagcc ccactctctg gtgcgtcgcc
     5641 cgttggtgct tatcgtcttg ttcgctcaaa tcactttcca tgcggcgctc aggcgcgtcc
     5701 atgcaagctg tacgcgccag ccggcaagac tacccttaag cgaatgcaaa gatcgacctt
     5761 gtgaccgccg atgaggacgg ctgcacagag gtagctttgt gtcttgggct atatcttgta
     5821 cgctcttttg ttcccggtac aagatgtagt agcatcttcg tcgtaccaac cctggtacgc
     5881 acggagatga ggtgtcaatc aggcagtgcc ggcgtgaccg ccatgccgga tgcgaaaatc
     5941 ggggacaggc ggggcgcgca tccctctcac gcgctcacgc ccgtcggccg cgatcaaggt
     6001 ccgacggcac ccgagacgca gtccgctcgc attgcaacaa ggcaggttga atcgatatgc
     6061 gacaatgacg ccgtagacga ttcaggtacc ggcctgttcg gcctgattcc tcctctgact
     6121 ttatgccgcc cggactccgg gcggcttttt tccgtcgggt gcgccaatgc cgaataacaa
     6181 caaccaaact ggcaaaggac tgtcgcccct cgcgtacgtc gaactgaagc ccggccagga
     6241 atattctccc tacgttcccg cc
//
LOCUS       DASUOM010000234         4615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1712562,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000234 DASUOM010000000
VERSION     DASUOM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712562"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VFY27_04830"
     CDS             complement(<1..1018)
                     /locus_tag="VFY27_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764870.1"
                     /translation="MSIDFGKSIRTSSVWMLAFIGTAALWAPGANASTAEMEALRQEL
                     NELRDDYEARISNLEQRLAVAEQNARQANTAVAQLGTAPAPSAPSSRADATGNSAFNP
                     AIGVIFQGQAWSYGNDPEAYAVQGFPFGGEAGPAGEGLSLGETEIDISANVDDKFTAW
                     LTVPVVIEDGESGIEIEEAWLETLSLPGGVSARFGRFFSRIGYLNDKHAHSWDFVDQP
                     LPYQAFLGDQYLDDGVQLRWIAPTSQFLEVGAEVLRGGRYPAAGAGNSGFGARSIFVN
                     TGGDVGTDHSWLAGLSFLDGESIERPSGDEDDPFLFTGDSSTTIAQFVWKWAPQGNWR
                     QRNFV"
     gene            complement(1090..1368)
                     /locus_tag="VFY27_04835"
     CDS             complement(1090..1368)
                     /locus_tag="VFY27_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764871.1"
                     /translation="MLKVTRNQWLLVLLVVLISHAALTIHSGTHLALDKQGCELCTQQ
                     ANLAHAVPPDVSSSFDFSQFAPLAPSIASVPSSAALVPYHQRAPPRNA"
     gene            complement(1520..2806)
                     /locus_tag="VFY27_04840"
     CDS             complement(1520..2806)
                     /locus_tag="VFY27_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX5764872.1"
                     /translation="MSTFDLETVRSRFPALSLTDNGIPRIYFDNPAGTQVSRLVVERM
                     SQCLLGANANLGGYFATSRFANAVVDDAHAAMADFLNAPSPDEIIFGQNMTTLTLHIS
                     RSIGRQFRAGDEIILSRMDHDANVAPWVLLARDLDLKVRWLPFNLDTFEFDLSVLDDL
                     LNDRTRLVCVGGASNMTGTIHDIRTISAKARQAGAWTYIDAVQWVPHVPTDVQEIGCD
                     FLACSAYKFFGPHQGILWGRREVLERLEPYKVRPAPDSIPGAFETGTQSHEGMAGTAA
                     AVDYFAWIGKTMAQDYLKRNDRYEGRSRYVHAAMSCLFDYEITLARHLIDGLRQLPGV
                     KVNGITGADAMARRVPTVSFTAAGMSPADIAQALAKKNIFVWSGHNYAVEAARALNIY
                     DSGGGVRVGPVHYNSVEELDRLLAALEEILPRARVA"
     gene            complement(2821..3030)
                     /locus_tag="VFY27_04845"
     CDS             complement(2821..3030)
                     /locus_tag="VFY27_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764873.1"
                     /translation="MNLREQVESLLPAWERWYPSLFDAASDLGLIRAEVCDPNTLLLT
                     NRHARVRQRAEEFHRQKWGVEPEDK"
     gene            complement(3109..3447)
                     /locus_tag="VFY27_04850"
     CDS             complement(3109..3447)
                     /locus_tag="VFY27_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764874.1"
                     /translation="MAERLKDEYDAKLEALFRSAPLEDDGFSKRVVWRVRRGIWIRRW
                     TLPLAILVGGLIAAKPAAELLLGMAEILAVLPEDVRNLELDALPQVTTFIFGGMVAAL
                     IGLFLKGLEE"
     gene            complement(3440..3928)
                     /locus_tag="VFY27_04855"
     CDS             complement(3440..3928)
                     /locus_tag="VFY27_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5764875.1"
                     /translation="MVRRHQSQVRTFLRRLTRDYELADDLAQDAFVHAWDRLHTYSGS
                     GSFIGWLLKVAYTTFLQSKRKSTRYSEVLQEMGHATDTERETTSQPNEEIGDLDRFLA
                     VLTEEERAVMIMSYAFGLSHREIGDAASLPVGTVKSIIFRGKEKIREAFAINDREDHH
                     YG"
     gene            complement(4015..4380)
                     /locus_tag="VFY27_04860"
     CDS             complement(4015..4380)
                     /locus_tag="VFY27_04860"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX5764876.1"
                     /translation="MDETWIPIVMFIAMGAVFVFFFWFRYRGRAEMQQTIRTALERGQ
                     ELTPEIVDRLGQPKPREFADRRRAIIAIAIGVAVAAFGFILGEEDAVRPFLAISAFPF
                     TLGIGYLIISRFSDHKPSV"
BASE COUNT          863 a         1438 c         1400 g          914 t
ORIGIN      
        1 aaacgaagtt tcgctgtcgc cagtttccct gcggagccca cttccacacg aactgcgcga
       61 tggtcgtgga tgaatcgccg gtaaacagga acgggtcgtc ctcgtcgccc gaaggcctct
      121 cgatgctttc accatcaagg aaagacagcc cggcgagcca gctgtgatcc gtgccgacat
      181 cgcccccggt atttacgaaa atgctgcgtg cgccgaatcc tgaattgccc gcccccgccg
      241 cggggtaacg tcctccgcgc agtacttcgg caccgacctc caggaactgg ctggtcggcg
      301 caatccagcg aagctggacg ccatcatcga gatactggtc tcccaggaat gcctggtagg
      361 gcagcggctg gtcgacgaaa tcccacgagt gcgcgtgctt gtcgttcaga tagccgatac
      421 gtgagaagaa gcggccgaac cttgctgaca cgccgccggg caggctcagg gtctcgagcc
      481 atgcttcctc gatctcgatg ccggactcgc cgtcctcgat cactaccggg acggtgagcc
      541 aggctgtgaa cttgtcgtcg acgttggcgc tgatgtcgat ctcggtttca ccgagcgaaa
      601 gtccctcgcc tgcaggcccg gcttctccgc cgaaggggaa gccctgcacc gcgtatgctt
      661 ccgggtcgtt tccgtagctc catgcctggc cctgaaagat gacgccgatt gccgggttga
      721 aagcggagtt gccggttgcg tcggcgcggg atgacggcgc tgacggagct ggagccgtgc
      781 cgagttgggc cacggcggtg tttgcctgcc tcgcgttctg ctccgctacg gcaagtcttt
      841 gttcgaggtt cgagatccgt gcttcgtagt catcgcgcag ttcgtttagt tcctggcgca
      901 gcgcttccat ttctgcggtg ctcgcgtttg cgccgggtgc ccacagcgct gctgtgccga
      961 taaaggcaag catccataca ctcgaagtcc ggatgctctt tccaaagtcg atgctcatcg
     1021 cgtcgcctcc tgatcaaact gatagagatt gccgcaacgg caatccgctg cggctgcagt
     1081 cgtgacggat caggcgttac gcggaggggc tcgctggtgg tacggaacaa gagccgcgga
     1141 tgacggcacc gatgcgatcg agggtgccag cggtgcgaac tggctgaagt cgaatgacga
     1201 ggaaacgtcg ggcggtaccg catgtgcaag gttcgcttgc tgcgtgcaca gctcgcaacc
     1261 ctgtttgtcc agcgcgaggt gcgtgccgga atggatggtc aacgcggcat ggctgatcag
     1321 gacgaccagc agcacgagca gccattgatt tcgggttact ttcagcatgg gcggtatcgc
     1381 tcaatatact ggctcgaaag cggccacaag taaatgcacg caagggcaca atgtgaacct
     1441 cccggaagcg tggaatagac cgctgtagga gcccgccgtg cgggctccca caaccctctc
     1501 aaaggccgca gcggagccgt caggcgacgc gcgcgcgggg gaggatttcc tccagtgccg
     1561 cgaggagccg gtccagttct tcaacggagt tgtaatgtac cggcccgacc cggacgccgc
     1621 cgccgctgtc gtagatgttc aaggctcgcg ccgcttccac cgcgtagttg tggccgctcc
     1681 agacgaagat gttcttcttc gcgagcgcct gtgcgatgtc ggcgggtgac attcctgcag
     1741 ccgtgaagga caccgttggt acgcggcgcg ccatcgcgtc cgcgcctgtg atgccattca
     1801 ccttcacgcc gggcaattgc cgcaagccgt cgatcaggtg tcgcgcaagc gtaatctcgt
     1861 agtcgaacag gcaggacatc gcggcatgca cgtaacggct gcggccttcg taccggtcgt
     1921 tcctcttcaa atagtcctgg gccattgtct tgccgatcca cgcaaaataa tcgacggcgg
     1981 ccgcggttcc ggccatcccc tcatggctct gggtccccgt ttcgaacgcg ccggggatcg
     2041 agtcgggcgc cggccgtacc ttgtatggtt cgaggcgttc cagcacttcc cgtcgtcccc
     2101 acaggatgcc ctggtggggg ccgaaaaact tgtacgccga gcaagcgagg aaatcgcagc
     2161 cgatttcctg cacgtcggtc ggcacgtgcg gcacccactg gacggcatcg atgtaagtcc
     2221 acgcgccggc ttggcgggct ttggcgctga tcgtcctgat gtcgtgaatc gtgccggtca
     2281 tattgctggc gccgccgacg cacacgagcc tggttcgatc gttcagcaaa tcatccagca
     2341 ccgaaagatc gaactcgaac gtatccaggt tgaacgggag ccagcgcacc ttgagatcca
     2401 ggtcccgcgc caggagaacc cacggcgcta cgttcgcatc atgatccatc cgcgagagga
     2461 tgatttcgtc gccggcgcga aactgcctgc cgatcgaccg cgatatgtgc agcgtcagcg
     2521 tcgtcatgtt ctggccgaag atgatctcgt cgggtgatgg cgcattcagg aaatccgcca
     2581 tcgcggcgtg tgcatcatcg acaacggcgt tggcgaagcg cgaagtggcg aagtaaccgc
     2641 cgaggtttgc gttcgccccg agcaggcatt gcgacatcct ctccactacc agtcgggaga
     2701 cctgcgtgcc cgccggattg tcgaaataga tcctggggat tccgttgtcg gtcagcgaca
     2761 aggcaggaaa ccgggagcgg acggtctcga gatcgaaggt gctcattggc cggagcgcag
     2821 ttacttgtct tcgggctcca cgccccattt ctggcgatga aattcttccg ctctctggcg
     2881 cacgcgcgca tggcgattag tcagcagcag cgtgtttgga tcgcacacct ccgccctgat
     2941 cagccctaag tcactggcgg catcgaaaag gctcggatac cagcgctccc acgcaggcag
     3001 gaggctttcc acttgttcgc gaagattcat cggtcgaatg tatacgaccg ggcacgcggt
     3061 gacaacgtca cgggttccgt tgtcagaaaa tcgcgacagg acttgtgttt attcttcgag
     3121 gcccttgaga aatagcccga tcagtgcggc aaccatgcca ccgaagatga aggtcgttac
     3181 ctgcggcagc gcgtcgagct cgaggtttcg cacgtcttcc ggaagtacgg ccagtatctc
     3241 tgccatcccc aacagaagct ctgccgccgg cttcgcagcg ataagtccgc caaccaggat
     3301 tgcaagaggc agagtccagc gccggatcca gattccgcgg cgtacgcgcc agacgacgcg
     3361 cttactaaaa ccgtcgtctt cgaggggcgc cgaacgaaac agcgcttcga gcttcgcatc
     3421 gtattcgtct ttcagccgct cagccataat gatgatcttc ccggtcattg atcgcgaagg
     3481 cttcccggat tttttccttg ccgcgaaaga tgatcgactt caccgtgccg accggaagac
     3541 tcgcagcgtc gccaatctcc ctgtgcgaca gcccgaaggc atacgacatg atcatcaccg
     3601 cgcgctcctc ctccgtcaat accgccagga acctgtcgag atcgccgatc tcctcgttcg
     3661 gctgcgacgt cgtctctctt tccgtatcgg tcgcatgacc catctcctgc aacacctcgc
     3721 tgtagcgcgt cgacttgcgt ttcgactgca ggaaggtggt gtacgccacc ttcagcagcc
     3781 agccgatgaa cgagccgctg ccggaatagg tgtgcagccg gtcccatgca tgcacgaacg
     3841 catcctgcgc caggtcgtcc gccagttcgt agtcgcgagt aagccttcgc aggaaggttc
     3901 gtacctgcga ctggtgccgc ctcaccaact cgccgaaggc gcgcggatcg ggctgcgcga
     3961 ccgcccgggc gacgagatcc ttgtcgtcgg caccggtgta gcgggtttcg cgtttcagac
     4021 tgatggcttg tggtcactga atcgggaaat gatgaggtag ccgatcccta gcgtgaacgg
     4081 gaatgccgag atcgccagga acggacggac cgcgtcttcc tcgcccagta taaagccgaa
     4141 cgccgccacg gcgacgccga tcgcaatcgc gatgatagcg cgcctgcggt cggcgaattc
     4201 tctgggcttt ggctgcccca gccggtcgac gatttccggg gtcagttcct gcccgcgctc
     4261 cagcgcggtg cggatcgtct gctgcatctc cgcccggccc cgatagcgaa accagaaaaa
     4321 aaacacgaaa accgccccca tcgcgatgaa catgacgatc ggtatccagg tctcatccat
     4381 ttagaaactc ctgagctttt tgcgggcaat agcgccttgt accgggaaga tgcgccccgt
     4441 ccagcctttg gatgcaagtt tatgcctggg atgccgcgtt attttgatcc tggcagcctg
     4501 gtgtgcagtc tccaggtctt tcgccgcccc cgagggcgcg cgtaggagcc cgccatgcgg
     4561 gcgagtggga tggacgttag cgcactcgcc cgcatggcgg ctcctaccgc gcttc
//
LOCUS       DASUOM010000235         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1244157,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000235 DASUOM010000000
VERSION     DASUOM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244157"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="VFY27_04865"
     CDS             <1..1803
                     /locus_tag="VFY27_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX5764877.1"
                     /translation="SFRDVQAGFVNVLDAVAVSGQKGPLVVVYDIDASAIRAYRLNSD
                     TLQLDDVMKEPIPLNDEMTGMCHYLSPLSGSHYIFAATDGGLTLQYELFATGGGIAGQ
                     LLRTIPTGKDSGFCAVDQKAGMLYISEEGMGIWQLGAEPESDTVREPVDLRAPWGNLS
                     DEIKGVAVYNHGGEVSWLVAADVGEGRLAAYRLPGLEAVGTITVDGLGEAEGITATNA
                     AFGADYPQGLLAIADEDESDGATDLKLVPWNAFAEALGIEATVATTEGEATAAPAVAR
                     PMVETEPVGTFGDAADDPAIWIHPTDPERSLVIGTNKKRGLNVYDLDGRTVQTLADGN
                     MNNVDLRYDFPLGGEQVALVTASNRSTDSISVYKVDEDALQLVEVADGVIPTGFVDPY
                     GLCMYRSPSTGEYYVFINEGGEGNFRQWRLFDNGQGRVSAEQVREFPVGSQTEGCVAD
                     DELGFLYVGEEGNALWKYSAEPDAGADRVMIDSTGDNGHLDADVEGVALWIGENGTGY
                     LVASNQGADNYAVYRREGDNEYVGHFHIIANPEPGIDGASETDGLEVTSTPLGNRYPA
                     GLLVVQDGRNIAPEEPQNFKFVSWQDVAHALGVE"
     gene            complement(2066..2941)
                     /gene="iolE"
                     /locus_tag="VFY27_04870"
     CDS             complement(2066..2941)
                     /gene="iolE"
                     /locus_tag="VFY27_04870"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEX5764878.1"
                     /translation="MSIRIGINPLTWTNDDLPALGDEISLETCLTEAAAAGYSGVELG
                     RKFPRQADVLGEILSKHKLALVSGWYSLHLDDRNADAEFAAMRPHFDLLHALGSNVMV
                     CAEVTRAVHGDLNVRLSRRPQFSTSEFTRFAERLSALAARMRAEGMQLAYHHHMGTVI
                     QTEDEVDALMTACSPDVQLLLDTGHLTFAGGDPIRAANNHSTRIAHLHCKDIRPDVLR
                     RCRNADTSFLEAVLQGVFTVPGDGCIDFAAVLKPLLAGNYEGWIVVEAEQDPAVADPV
                     TYANLGYRTLRALTR"
     gene            complement(2938..>4835)
                     /gene="iolD"
                     /locus_tag="VFY27_04875"
     CDS             complement(2938..>4835)
                     /gene="iolD"
                     /locus_tag="VFY27_04875"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX5764879.1"
                     /translation="CLVLCQRSEARDRLTSERYRGANQMAQKTVRLTMAQALVRYLAA
                     QRTEVAGEEVPLVAGIFAIFGHGNVSGLGEALQNRRDDLPVYRAHNEQAMAHAAIAYA
                     KANKRRRFMACTTSIGPGATNMVTAAALAHVNRLPLLLLPGETFASRRPDPVLQQVED
                     FNEPGITANDCFRPVARFWDRIVRPEQLITSLPQAMSVLTDPADCGPVVLALPQDVQA
                     EAFDYPASMFEETVRSIEKPRPDERRLLQAVDWLRTAKRPLLIVGGGVHYSDAFAALD
                     AFVTRLGIPFAETQAGKGALPWNHPMNAGAIGVTGSSAANRLAAEADLVLAVGTRLQD
                     FTTASNTLFAAKPRMIGINVNRHDAIKRSALPLRGDAALTLAELDAALPDLKPRSSWT
                     DLAAASIAEWNQAVEAATRVTVADGLPGDADVLGVVNRSAGPDATVVCAAGGLPGELH
                     KLWRAHGPDSYHLEYGYSCMGYEIAGGIGVKLAKPERNVIVLVGDGSYLMMNSEIATS
                     VMLGLKLTIVLLDNRGFGCINRLQQSCGGDSFNNLLPKTPDIDFASHARSLGAEARVV
                     RNLQELEQALQEVSAAARTQVIVIETDPAKSTSAGGAWWDVPVPEVSERPEVQQAFAA
                     YDKARNE"
BASE COUNT          918 a         1527 c         1553 g          837 t
ORIGIN      
        1 agcttccggg atgtgcaggc aggattcgtg aacgtgctcg atgccgtcgc ggtttcagga
       61 cagaaagggc cgctggtcgt ggtgtacgac attgatgcgt ccgcgataag agcgtacagg
      121 ttgaacagcg atacgctgca actcgatgac gtgatgaaag aaccgatccc gttaaacgat
      181 gaaatgaccg gcatgtgcca ctacttgagc ccactgtccg gcagccacta catctttgcc
      241 gcgacggacg gcggtctcac gcttcagtac gaactgttcg caacgggtgg gggcatagcc
      301 gggcagctgc tgcgcactat cccgaccggc aaggactccg gcttctgcgc cgtcgatcaa
      361 aaggcaggca tgctctacat cagtgaagag ggcatgggca tctggcagct gggcgccgaa
      421 ccggagagcg acacggtgcg cgagccggtc gacctgcgtg caccctgggg aaacctgagc
      481 gacgagatca agggagttgc cgtctacaac cacggcggcg aggtctcctg gctcgtggca
      541 gcggacgtcg gcgaaggccg cctcgccgca taccgcctgc ccggtctcga ggcagtgggc
      601 accataacgg tcgacggtct cggggaagcc gagggcatta ccgcaacgaa cgccgcgttc
      661 ggcgccgact acccgcaggg gctcctggcg atcgccgacg aagacgaaag cgacggcgcg
      721 accgacctca agcttgtgcc gtggaatgca tttgccgaag cgctgggaat agaagcaacc
      781 gtcgccacaa ccgagggcga ggcaaccgcc gctccggccg tggcaaggcc gatggtcgag
      841 acagaacccg tcgggacctt cggcgacgcg gcggacgacc ccgccatatg gatccacccg
      901 accgaccccg agcgaagcct ggtcatcggt accaacaaga agcgcggcct gaacgtctac
      961 gacctcgatg gccgcaccgt gcagacgctc gcggacggca acatgaacaa cgtcgacctc
     1021 cgctacgatt ttccgcttgg cggcgagcag gtcgcactgg taacggccag caaccgcagc
     1081 accgacagta tctccgtcta caaggtagac gaagacgcct tgcagctcgt cgaagtggcc
     1141 gacggcgtca tcccgaccgg cttcgtcgac ccctacggcc tctgcatgta caggagcccc
     1201 tccaccggcg agtactacgt cttcatcaac gaaggcggcg agggaaactt ccgccaatgg
     1261 cgactgttcg acaacggaca gggccgtgtc agcgccgaac aggtgcggga attccctgtt
     1321 ggttcgcaaa ccgaaggctg tgtcgccgac gacgaactcg gcttcctcta tgtcggcgaa
     1381 gaaggcaacg cgctctggaa atactccgcc gagcccgatg caggcgccga cagggtcatg
     1441 atcgactcca ccggcgacaa cggtcacctc gatgccgacg tcgaaggcgt tgcactgtgg
     1501 atcggcgaga acggcaccgg ctacctcgtc gcctcaaacc agggcgccga caactacgcc
     1561 gtctaccgcc gcgagggcga caacgaatac gtcggccact tccatatcat cgccaacccc
     1621 gaaccgggca tcgacggcgc ctctgaaacc gacggcctcg aagtcaccag cacaccttta
     1681 ggcaaccgct atcccgccgg ccttctcgtc gtacaggacg gccgcaacat cgcaccggaa
     1741 gaaccgcaga acttcaagtt cgtctcatgg caggacgtgg cacacgcgct gggagtcgag
     1801 tagggctaac gcttgaactg caacgctaca tctatgtcgt tggcgggagg gtcagcgagc
     1861 attatccatg aacatgtgaa gttggtagtg gtgcgaatgc ccggcgggtg ggaggcctgt
     1921 cctgaaaggc ctcccttccc cggccgcggc tccgcaccac aactcccact ttcatgtgtg
     1981 atgcgtgcca cgcagacctc acagaccgcc aaccaccgcc cccaaaacag ccacaaagcc
     2041 cacaggaagc gtgccaccaa ctgaactacc gcgtcagggc acgcaaagtg cgatagccga
     2101 ggttcgcata cgtaacggga tccgcaaccg caggatcctg ctccgcctcc acaacgatcc
     2161 agccttcgta gttgccagcc agcaggggct tcagcacggc ggcgaagtcg atgcagccgt
     2221 cgccggggac ggtaaagaca ccctgcagca ccgcttcgag gaagctcgta tcggcgttgc
     2281 ggcagcggcg caggacgtcg gggcgtatgt ccttgcagtg cagatgggcg atgcgggtgg
     2341 agtggttgtt ggcggcgcgg atgggatcgc cgccggcgaa ggtgaggtga ccggtatcga
     2401 ggagcagctg gacgtcaggt gaacaggcgg tcatgagggc gtcgacttca tcttcggtct
     2461 gtatcacggt gcccatgtgg tggtgatagg cgagctgcat gccttccgcg cgcatgcgag
     2521 cggcgagcgc agagaggcgt tccgcgaagc gcgtgaattc ggaggttgag aactgcgggc
     2581 gacgtgaaag ccgcacgttc aggtcgccgt ggactgcgcg cgtgacctcg gcacagacca
     2641 tgacgttgga acccaaggca tggagcaggt cgaagtgcgg acgcatggcg gcgaactcgg
     2701 cgtcggcgtt gcggtcgtca aggtgcaggc tgtaccagcc ggaaaccagc gccagcttgt
     2761 gcttcgacag gatctcgccg aggacgtcgg cctgtcgcgg gaacttgcgg cccagttcta
     2821 cgccgctgta gcccgcggcc gctgcttcgg tcaggcaggt ttcgaggctg atctcatcgc
     2881 ccagcgcggg caggtcgtcg ttggtccacg taagcggatt gatgccgatg cgtatgctca
     2941 ttcatttcgt gccttgtcat aggcggcaaa cgcctgttgc acctcgggtc gctcggacac
     3001 ttccggaacc ggtacatccc accacgcgcc gccggctgag gtcgacttcg ccggatctgt
     3061 ttcgatgacg atgacctgtg ttcttgcggc agcggagact tcttgcagtg cctgctccag
     3121 ttcttgcagg ttgcgcacca cccgtgcttc ggccccgagc gaccgggcgt gtgaagcaaa
     3181 gtcgatatcc ggcgttttcg gcagcaggtt gttgaacgaa tcgccgccgc aggattgctg
     3241 cagccggttg atgcagccga agccgcggtt gtcgagcagc acgatcgtca gtttgagtcc
     3301 gagcatgact gaagtggcga tctcggaatt catcatgaga tagctgccgt cgccgacgag
     3361 cacgatgacg ttccgttcgg gcttcgcgag cttcacgccg atcccgcctg cgatctcgta
     3421 gcccatgcag gaatagccgt attcgaggtg atagctgtcg ggtccgtgcg cgcgccacag
     3481 cttgtgcagc tcgcccggca atccgccggc tgcgcacacg acggttgcat ccggacccgc
     3541 ggagcggttt accaccccta gcacgtcggc atcgcctggc aacccgtcag cgacagttac
     3601 gcgggtcgcc gcctcgacgg cctggttcca ttccgcaatt gacgctgccg cgaggtctgt
     3661 ccacgaagaa cgtggtttta ggtccggcag cgctgcgtcg agttcggcca acgtgagagc
     3721 ggcgtcaccg cgcagcggaa gtgcgcttct cttgatggcg tcatgccggt tcacgttgat
     3781 gccgatcatg cgcggcttcg cggcgaagag cgtgttggat gcagtggtga agtcctgcag
     3841 gcgcgtgccg accgccagta cgaggtcggc ttcggctgca agccggttcg cagcggagct
     3901 tccggtaacg ccgatcgcgc cggcgttcat cggatggttc cacggaagcg cgcctttgcc
     3961 ggcctgcgtc tccgcgaagg gaatgccgag ccgggtgacg aacgcatcga gtgcggcaaa
     4021 cgcgtccgag taatgcacgc cgccgccgac gatcaggagc ggccgctttg ccgtcctgag
     4081 ccagtccact gcctgcaaca gcctgcgttc atccggccgc ggcttctcga tgctgcgcac
     4141 cgtctcttcg aacatcgaag ccggataatc gaaggcttcc gcctggacat cctgcggcag
     4201 cgccagcacg accggtccgc aatcggccgg gtccgtcagc acggacattg cctgcggcag
     4261 cgaagtaatg agttgctccg ggcgcacgat gcggtcccag aagcgcgcca ccggcctgaa
     4321 gcagtcgttg gcagtgatgc cgggctcatt gaaatcctcg acctgctgca acacgggatc
     4381 ggggcggcgg cttgcaaacg tctcgcccgg cagcagcaaa agcggcaggc gattcacgtg
     4441 cgccagcgcg gccgcggtca ccatgttggt ggcgccgggc ccgatcgatg tcgtgcaggc
     4501 catgaaccgc cggcgcttgt tcgctttggc gtaagcaatc gcagcgtgag ccatcgcctg
     4561 ttcattgtgc gcgcggtaca ccgggaggtc atcacggcgg ttttgcagcg cttcgccgag
     4621 acccgacacg ttgccgtgac cgaagatggc gaaaatgccg gcgacgagcg gtacttcctc
     4681 gcctgcgact tctgtgcgct gcgccgcgag atatcggacc agcgcctgcg ccatcgtcag
     4741 tcgaaccgtt ttttgcgcca tctgattagc tccgcgatat cgttcgcttg tcagccggtc
     4801 gcgcgcttcg cttcgctggc agagcaccag acatc
//
LOCUS       DASUOM010000236         6095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_814264,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000236 DASUOM010000000
VERSION     DASUOM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814264"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..897
                     /locus_tag="VFY27_04880"
     CDS             124..897
                     /locus_tag="VFY27_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5764880.1"
                     /translation="MNLRLNWVAFKTLVTKEFIRVIRIWLQTIVPPAITMTLYFIIFG
                     SLIGRRIGSMDGYDYMQYIAPGLIMMSVITNSYGNVVSSFFGAKFARHIEEMMVSPMS
                     NATIILGHVAGGVIRGLLVGAMVTVVALGFTRLDVQHPLITFSMVLLSSIIFSLAGFI
                     NAVFARKFDDISIIPTFVLTPLTYLGGVFYSISLLPEVWQKVSLLNPILYMVNAFRYG
                     ILGTSDIEIGHAYLIVALFVIGLFSVSMILMNRGIGMRE"
     gene            909..1619
                     /locus_tag="VFY27_04885"
     CDS             909..1619
                     /locus_tag="VFY27_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5764881.1"
                     /translation="MCGRFAFYSPAEAAAELFGFEPPFEIDESRDGLGRAKQDARGGP
                     RYNIAPTELIPVVRRARPEGREFVMLRWGLVPYWAKEPSIGNRMINARAETLADKPAY
                     RNAYEKRRCLVLADGFYEWRKEASGKSPWFISLVSGEPFAFAGLWEAWIDRSSGERLE
                     TATIVTTAATGFLRELHDRMPVVLQPETAGAWLSGSPGVLENVTANPPEFQAWPVSRA
                     VNNSRSDGPELIERVASE"
     gene            complement(1638..2276)
                     /locus_tag="VFY27_04890"
     CDS             complement(1638..2276)
                     /locus_tag="VFY27_04890"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEX5764882.1"
                     /translation="MSFILDALKKSEAERQRKSVPGFADIPDARSEPRSQRWWWIIGG
                     LLTVNAAVLVSVFFVPDANDATEAAPVQQAAVSPPPVSERRPATATPKPFAEIVAEAK
                     KNRPLQDVPTTTEPQRQDSAPATTPASSRVTMSEQLATINEVRAEGGLQLPELHLDIH
                     VYSERPAERFVFINMAKYKEGDTLSEGPAVREIRTDGVVLEQSGTRFLMPRQ"
     gene            complement(2286..3956)
                     /locus_tag="VFY27_04895"
     CDS             complement(2286..3956)
                     /locus_tag="VFY27_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5764883.1"
                     /translation="MYTKFFGLNEKPFAITPDPRYLFMSERHGEGLAHLVYGVTESGG
                     FIQLTGEVGTGKTTLVRTLLGQMPSEIDVALILNPQLTALEFLQAICEELGVPLPDDR
                     NSSKALVDALNLHLLNAHARGRRTILLVDEAQNLSAGVLEQLRLLTNLETAKQKLLQI
                     ILIAQPELRELLSQNNLRQLAQRVTGRYHLEPLSREEAARYIDHRVKVAGGLTEIFDQ
                     GAKREVYRLSGGIPRLMNVICDRALLGAYSRESRKVNKWMVRRAAAEVSGQKLTPMAL
                     KWAVPVVAAIGIVLAMVGLWTLIERSGPEPDTGEVLRIPVAAAGPEAGPGDGRSPGQT
                     GDPPSMGTEARELNLEDRLRAAGAGTSVESAMSTLFGLWNVDFDESTGSTACQQAEAK
                     GLSCYFNRGSWSGIRQLDRPVMLTLTDSHGDTHQPVLVSINEEEGELAIGEGRVTYSL
                     DEISEMWFGQYLLVWRPPNGRNDIMRPGMRDPNVLWLRQSLAALGAEYRPESLDSDLF
                     DDDLEQKLMQFQRTHRLRIDGIAGEQTQILLHSLLGPGGTPQLVAQSR"
     gene            complement(4183..5382)
                     /locus_tag="VFY27_04900"
     CDS             complement(4183..5382)
                     /locus_tag="VFY27_04900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5764884.1"
                     /translation="MTPVQRRHILHVDMDAFYASVEQRDRPKLRGKPVVVGGDARGVV
                     AAASYEARRFGIRSAMPMREAERRCPDLIRIPVRMSCYKSVSDEVFAIFRDVTPLVEG
                     LSLDEAFLDVTGSLNLFGTAEKIAATIKARILSQCELTASVGVAPNKLVAKIASDLDK
                     PDGLVVVTEQNVHAILDPLPVVVIPGIGPTTKLRLDDAGIHTIADLRAAPDRVLEPLF
                     GRYTQRTRERASGIDDRPVQPVRTEKSISAEETFERDLAERREMQRELMQLCERTGAR
                     LRARSLVAGTVQLKIRQGDFTTFTRQQALKPPGNATDTLFGIARRLLDEWLSDCPGSR
                     VRLLGVGGSELSPASQGDLFALDENHPTQRGGATSQLDRAVDEIRVRFGDRSLNRARG
                     LDPRQPR"
     gene            complement(5379..>6095)
                     /locus_tag="VFY27_04905"
     CDS             complement(5379..>6095)
                     /locus_tag="VFY27_04905"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5764885.1"
                     /translation="ECLDLDPELLDGPAVAIELIHAFSLVHDDLPAMDNDDLRRGELT
                     VHRKFDEACAILAADALQPLAFSVLCSAPGVGPARANALVQLVADACGSRGMTGGQSI
                     DLSSEGMALSRAELEQMHALKTGALIRASVMSACCLAPALSAGEMASLERFASTIGLA
                     FQIKDDILDVEGETEVIGKKAGADQALRKATWPSLFGLEEAKARCNDLLENALSALDR
                     IDRDAGTLRWLARYIVERAQ"
BASE COUNT         1052 a         1881 c         1939 g         1223 t
ORIGIN      
        1 gtcaggcggc ggtttagccg gatatgtcac cgattcgcgg gaatctcggg cagccagttg
       61 tgcggacaag gggccagcga gaaaccgcga attcggtgag atatccgggt tagggagctt
      121 gtgttgaatc tcagactgaa ctgggtcgcg ttcaagacgc tggtcacgaa ggaattcatt
      181 cgcgtcatcc gcatctggct gcagaccatt gtcccgcccg cgatcaccat gacgctctac
      241 ttcattatct tcggcagcct gatcggccgg cgcatcggca gcatggacgg ctatgactac
      301 atgcagtaca tagcgccggg gctgatcatg atgtcggtga ttacgaactc ttacggcaac
      361 gtagtctcat cgttcttcgg cgccaagttt gcgcgtcaca tcgaggaaat gatggtctcc
      421 ccgatgtcga acgccaccat tatcctgggg cacgtcgccg gcggcgtgat tcgcgggttg
      481 ctggtggggg ccatggtgac ggtggtagcg ctcggcttca cacgtctcga tgtgcagcac
      541 cctctgatta cattctcgat ggtgctgctg tcctcgatca tcttctccct ggcgggattc
      601 atcaacgcgg tcttcgcacg gaaattcgat gatatttcca tcataccgac cttcgtactg
      661 acaccgctca cctacctcgg cggcgtgttc tactccatct cgctgttgcc cgaggtgtgg
      721 cagaaggttt ccctgctgaa cccgatcctc tacatggtaa acgcattccg ttacggcatt
      781 ctcggcactt cggacataga aatcgggcac gcatacctga tcgtggcgct gttcgtcatc
      841 gggctctttt cggtaagcat gatcctgatg aatcgcggca tcggcatgag ggaataggcg
      901 gtaccggcat gtgcggaagg tttgctttct actcgcccgc cgaagctgcg gcggagctgt
      961 tcgggttcga gcccccgttc gagatcgacg agtccaggga tggactcggt cgagcgaagc
     1021 aggatgcccg aggcgggccg cgatataaca ttgctcccac ggagctgata ccggttgtca
     1081 ggcgcgcgcg ccctgagggc cgcgaattcg tcatgctgcg ctgggggctc gtgccgtact
     1141 gggcgaaaga accgtcgatc ggcaatcgca tgatcaatgc acgcgcagag acactggcgg
     1201 acaaacctgc ttatcgcaat gcctatgaga agcgccgctg cctggtgctt gccgacgggt
     1261 tttacgagtg gcgaaaggag gcaagcggca aatcgccatg gtttatcagc ctggtgagcg
     1321 gcgagccgtt cgcctttgcc ggtctttggg aagcgtggat tgaccggagt tcaggtgaac
     1381 gcctggagac cgcgactatc gtcactaccg ccgccaccgg tttccttaga gaactgcatg
     1441 accggatgcc tgtcgtactg cagcccgaaa ccgcgggcgc gtggctttca ggcagtcccg
     1501 gggtcctcga gaatgtcacc gccaacccgc cggaattcca ggcatggccg gtaagccgcg
     1561 cggtcaacaa ctcgcgctcc gatggcccgg agctgatcga gcgggttgcg agcgagtgag
     1621 gcgataatgc ctcccggcta ctgccgcggc atgaggaatc gcgtgccact ctgctccagc
     1681 accaccccgt cggtacggat ctcccggacg gctggtccct cgctcaaggt gtcgccttcc
     1741 ttgtacttcg ccatgttgat gaaaacgaag cgttcggcgg gtctctcgct gtagacgtgg
     1801 atgtcgaggt ggagctcggg taactggaga ccgccttcgg cgcgaacctc gttgatggta
     1861 gcgagttgct ccgacatcgt gacgcgggaa cttgcaggag tggtcgcggg cgccgaatcc
     1921 tggcgttgcg gctccgtcgt ggtaggcaca tcctgcagcg ggcgattctt cttcgcttcg
     1981 gcgacgatct ccgcgaaagg tttgggcgtc gcagtcgcgg gcctccgttc gctaacgggc
     2041 ggagggctga ccgcggcctg ttgcacaggc gccgcttcgg ttgcatcgtt tgcgtccggc
     2101 acgaagaaga cgctgacaag gactgcggca ttgacggtga ggagcccgcc gatgatccac
     2161 caccagcgct gcgaccgcgg ttcgctcctc gcatccggta tgtcggcgaa tcccggcacg
     2221 ctcttgcgtt gccgttcggc ttccgatttt ttcagtgcat cgagaatgaa tgacatcgga
     2281 cgaagctacc ggctctgagc gacgagctgc ggcgtgccgc ctgggccgag aagagagtgc
     2341 aggaggatct gcgtctgttc accggcaatg ccgtcgatgc gcagccggtg ggttcgctgg
     2401 aactgcatca gcttttgctc gaggtcgtca tcgaacaggt cggagtccag gctttccggc
     2461 cggtactccg cgccgagcgc tgcgaggctc tgccgcagcc agagcacgtt cgggtcgcgc
     2521 atccccggcc gcatgatgtc gttcctgccg ttaggcggac gccacaccag caggtactgg
     2581 ccgaaccaca tctcggaaat ttcgtcgagg gaataggtaa cgcggccttc gccgatcgca
     2641 agctctcctt cctcctcgtt gatggagacc agcaccggct ggtgtgtgtc gccatgggaa
     2701 tcggtcagcg tgagcatcac cggccggtcc agttgccgta tgccactcca cgatccccgg
     2761 ttgaaatagc agctcaagcc cttggcttcc gcttgctggc aggccgtcga accggtggac
     2821 tcgtcgaagt cgacgttcca caggccgaac agggtactca tcgcgctctc gacactggtg
     2881 ccggctcccg cggcacgaag ccgatcttcc aggttgagtt cgcgagcttc ggtgcccatg
     2941 gaaggcgggt cgccggtttg cccaggggat cgaccgtctc ccgggccagc ttcagggccc
     3001 gcggcggcga cgggaatgcg caggacctcg cccgtgtccg gttccggccc gctccgctcg
     3061 atcagcgtcc agagaccgac cattgccagc acgatcccga ttgcggcgac taccggtact
     3121 gcccacttca gggccatcgg cgtcagcttc tgtccggaaa cttcagcggc ggcgcggcgc
     3181 accatccact tgtttacctt gcggctttcg cggctgtatg cgccgagcag cgccctgtcg
     3241 cagatgacgt tcatcagccg cggaatgccg cctgagaggc gatatacctc gcgcttcgcg
     3301 ccctggtcaa aaatctcggt gaggccgccg gctaccttta cccgatgatc gatgtaacga
     3361 gcggcctctt ctcgcgacag cggttccagg tggtatcgtc cggtcacgcg ttgcgcgagc
     3421 tggcgcaggt tgttttgcga cagtagctcc cgcagttccg gttgcgcgat aaggataatc
     3481 tgcaggagct tttgctttgc cgtttccagg ttcgtcagga gccgcaattg ctccagcacg
     3541 ccggcagaaa ggttctgcgc ctcgtctacc agaagtatgg ttcttcgccc gcgtgcgtgc
     3601 gcgttgagga gatgcaggtt cagcgcgtcg accagcgctt tgctgctgtt gcggtcgtcg
     3661 ggaagcggca cgccgagctc ttcgcagatc gcctggagaa actcgagcgc tgtcagctgc
     3721 gggttgagga tgagggcaac gtcgatctcg gatggcatct ggcccagcag ggtccgcacc
     3781 agggtggtct tgccggtgcc gacttcgcca gttagctgga tgaagccgcc gctttcggtg
     3841 accccgtaga cgaggtgagc gagtccctcg ccgtgccgtt cgctcatgaa caggtaccgc
     3901 gggtccggag taatcgcgaa cggcttttcg ttcaggccaa agaatttagt gtacatagtt
     3961 gcggcaatgc gttgcggcga tggcgatcca gcccggtgtc gcgggagccg ccgacaagtc
     4021 tatcagttcc tggcagcctg tcggtcacgg taccaggccc agcgaacggt caggaagccg
     4081 gaagaagcct gcaggagcct gccatgaggg cgagtacggt caagccgcag cgaactcgcc
     4141 ggcatggcgg gctcctgaaa gtgcgctgaa catgactccg agctaacgtg gctggcgcgg
     4201 gtccagccct cttgcacggt tcagcgacct gtctccaaac cgcacccgga tctcgtcgac
     4261 cgcgcggtcc agctgcgagg tcgcgccacc tctttgtgtc ggatggtttt cgtccagggc
     4321 gaacaggtcg ccctggctgg cgggcgaaag ttcgctcccg ccgactccca ggaggcggac
     4381 ccgcgagccc gggcaatcag acagccattc gtcgagcagg cgccgcgcga tgccgaacag
     4441 cgtgtccgtg gcattgccgg gcggcttcag cgcctgttgc cgggtgaatg tggtgaagtc
     4501 gccctggcga atcttcagct gcaccgtgcc ggcgacgagt gacctggcgc gcaggcgggc
     4561 accggtgcgt tcgcaaagtt gcatgagttc ccgctgcatt tcccggcgct ccgcgaggtc
     4621 gcgctcgaac gtctcctctg cgctgatcga tttttccgtc cgcacgggct ggaccggacg
     4681 gtcgtcgatc ccggacgccc gctcccgggt tcgctgcgta tagcgcccga acaaaggttc
     4741 aaggacacgg tcgggagcag ctctcagatc cgcgatcgtg tggatacctg catcatcgag
     4801 gcgcaatttc gtagtcgggc cgatgccggg aatcaccacg acggggagcg ggtcgaggat
     4861 cgcgtgcacg ttctgttcgg taacgactac cagcccgtca ggcttgtcga ggtcggatgc
     4921 aatcttcgct accagcttgt tcggcgcgac tccgacggat gccgtcaatt cgcattggga
     4981 caggatgcgc gccttgatcg tagccgcgat cttttctgcg gttccgaaca ggttaaggct
     5041 tccggtaacg tcgagaaaag cttcgtccag cgacaggcct tccaccaggg gcgtgacgtc
     5101 ccggaatata gcgaacacct cgtccgagac agacttgtaa caggacatgc gcaccgggat
     5161 gcgaatcagg tccgggcagc gtcgctcggc ttcccgcatc ggcattgcag agcgtatgcc
     5221 gaaccttctc gcctcgtagc tcgcggcggc cacgactccg cgcgcgtcgc cgcctaccac
     5281 gaccggcttg ccgcgaagtt tcggccggtc ccgctgctcc acggaagcgt agaaggcgtc
     5341 catgtctaca tggagtatgt gtcgacgttg caccggcgtc attgcgccct ctcgacaatg
     5401 tatcgcgcca gccatcgtag cgtgcccgca tcccggtcga tgcggtccaa cgcggaaagc
     5461 gcattttcca gcagatcgtt gcagcgcgcc ttcgcctctt cgaggccgaa caacgaaggc
     5521 caggttgcct tgcgcagggc ctggtcggca ccggccttct tacctatcac ctctgtctcg
     5581 ccctcgacgt ccaggatgtc gtcctttatc tggaaggcga gaccgatcgt gctcgcgaag
     5641 cgctcgagcg atgccatttc cccggcggac agcgcgggtg cgaggcagca tgcagacatg
     5701 accgatgcac gaatcagcgc accggtcttc agggcatgca tctgctcgag ctccgctcgt
     5761 gacagggcca ttccttcgga agacaggtct attgactggc cgccggtcat gcctcgcgag
     5821 ccacaggcat cggcgaccag ttgcactagc gcattcgctc gtgccgggcc gacgccgggc
     5881 gcggagcaca gcacgctgaa cgccagtggc tgtagagcgt cggccgccag tattgcgcag
     5941 gcttcatcga acttccggtg aacggtcagc tcgccacggc gcaggtcgtc gttgtccatg
     6001 gccggcaaat cgtcgtgcac gagggagaac gcgtgaatca gctcgatggc gaccgccggt
     6061 ccatcgagca gctccgggtc gaggtcgagg cattc
//
LOCUS       DASUOM010000237         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1100559,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000237 DASUOM010000000
VERSION     DASUOM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100559"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFY27_04910"
     CDS             complement(<1..249)
                     /locus_tag="VFY27_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764886.1"
                     /translation="MNLLIGLPTMTLCLFLQSALFVVALRYYSRHESLVNDASWWSAL
                     IVINGVMTLLVVGNLIQLTIWAVVFMMLGEFEELAEAVY"
     gene            complement(255..1298)
                     /locus_tag="VFY27_04915"
     CDS             complement(255..1298)
                     /locus_tag="VFY27_04915"
                     /inference="COORDINATES: protein motif:HMM:NF022616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEX5764887.1"
                     /translation="MPIGARRTFRLGAVVALSLAFAYGLGIPLPFFAPLFGLLLTAVP
                     GPPMRLKGLLGLFLVVTITLGVGLVLTPLLGKYPVSAILTIAAGVYLSTYVSVGLRKG
                     LIGMLLTMGFTLIPAAGLVDYSVAVTVIDALLLGIGLAVVSQWIVYPFFPEDPRKEIA
                     ATPPAVDASQVSWIALRATLIVLPPVLMVFSNPSLYLPIIMKSVLLAQQESVVSARSA
                     GRALLGATFLAGLLAIVFWFALKICPSLWMFFLWMLLFGIYFAAKLYGVVASRFPPSF
                     WQDVVVNLLILLGPAVEDSATGKDPYEAFAVRFSLFVAVTLYAWLAIVALEWLRARRR
                     GVMPTLAPAPEDC"
     gene            complement(1311..2495)
                     /locus_tag="VFY27_04920"
     CDS             complement(1311..2495)
                     /locus_tag="VFY27_04920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5764888.1"
                     /translation="MSDRPAEDKVIVDEAPHDGGEEGTDVQDSQPQKEEASRSVRKGA
                     IGVALVIVLSLTWYVLADRYTPYTTQARIEGYIIGVAPEVAGTVKRVWVTNNQEVEEG
                     QPLFEIDSSQYQIALNRARSDFENAGRQVGAGSAAVNAARANLFAARANERKARQDAT
                     RLARLYKEDPGTISVRLLEVAQASHEAAQAQVTAAEADIQRAIEQMGGDDPANNTIIN
                     TALTAVAKAELDLANTLVKAPSRGVITDLKSDVGHFAGTGSPVLTLIAIHDVWVNAEF
                     TENNLGHVEVGTPVEILFDVLPGRVFRGEVRSIGLGVSGGRSEPAGTLPAIQNDRDWL
                     RQSQRFPVIIGFDTRQDEALRRQLRIGGQVSVIAYSEGTGLLRLLGKAYIRVASWLSY
                     VY"
     gene            complement(2488..>3622)
                     /locus_tag="VFY27_04925"
     CDS             complement(2488..>3622)
                     /locus_tag="VFY27_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5764889.1"
                     /translation="FDIGWELDFWGRFRRGIESANAAYFASITGQQDAQVLLSAQVAD
                     LYFAYWTTLLRIDIAQRNADIQQRSFEITEKIYEAGQGSELDLQQAKTQYLATLSTIP
                     ELEATLIKLQNALAALLGRTPGDVPELQSVKGELPAVDPVAIQGIPAQLLLRRPDIRT
                     AAWQIAAQSAQIGVAKADYYPAITLLGSIGWSADSLDASSDTSSFVVGPALSWNVFDY
                     GRIRNNVRLQDARLQQSIEIFQESVLQAAREIDDAAISVVKTGEQQRVLADSLRAAER
                     SLALANTRYQEGYADFQRVLDAQRALFAQADQELIIRSTHISAIIALYRSIGGGWTDM
                     SVEQMVPESIRDEMRSRTNWGDLLSEPLPRDDGPSGEPGTHNE"
BASE COUNT          727 a         1163 c         1063 g          669 t
ORIGIN      
        1 atacacggcc tcggccaatt cctcgaactc ccccagcatc atgaagacta ccgcccagat
       61 cgtcagctgg atgagattac cgacaaccag aagcgtcatg acgccgttga taacgatcag
      121 cgccgaccac cacgacgcat cgttcaccag gctctcgtgg cgtgaatagt agcgaagcgc
      181 cactacgaac aacgccgact gtaagaacaa gcacaatgtc atcgtgggca agccaatcag
      241 gagattcatg tgcatcaaca atcctccggg gcaggcgcca atgtcggcat cacaccgcga
      301 cggcgggcgc gcagccactc cagtgcgaca atcgcgagcc aggcgtagag cgtaacggca
      361 acgaacaggc tgaaacggac tgcgaacgcc tcgtacgggt ccttgccggt cgcgctgtcc
      421 tcgacggccg ggccgagcag aatcaggagg ttcacgacca cgtcttgcca gaaggaaggt
      481 ggaaaccgac tggcgacgac tccatacagc ttggccgcga agtagatgcc gaacagcagc
      541 atccacagga agaacatcca caaactcgga cagattttca gtgcgaacca gaacacgatc
      601 gcgagcaagc cggcgaggaa cgtggcgccg agcagcgcgc ggccggccga tcgcgcgctc
      661 acgaccgatt cctgctgcgc cagcagcacg gatttcatga taatgggcag gtacagggac
      721 ggattggaaa aaaccatcag tacgggaggc agtacgatca gcgttgcgcg cagcgcgatc
      781 cagctgacct ggctcgcatc gactgccggc ggcgttgccg cgatttcctt gcgcgggtcc
      841 tcgggaaaga acggatagac gatccactgg ctgacgaccg ccagcccgat gccgagcagc
      901 agggcgtcga taaccgtcac ggcaacggaa taatccacca accccgcggc cgggatcagc
      961 gtgaagccca tggtgagcag catgccaata aggcccttgc ggagccccac gctcacgtaa
     1021 gtactcagat acacccccgc cgcaatggtc agaatcgcag acacagggta tttgccgagg
     1081 aggggagtca gcaccagccc gacgccgagc gtgattgtga ccaccaggaa gaggccgagc
     1141 aggcccttca atcgcatagg cggtcccggc accgccgtga gcagaagacc gaacaggggc
     1201 gcgaaaaacg gcaacggtat tccaaggccg tatgcgaagg cgagcgacaa tgccacgaca
     1261 gcgccaaggc ggaacgtgcg cctggcgccg atcggcatca ccctggcacc tcaatagaca
     1321 taggatagcc agctcgcaac gcgaatatag gctttgccca aaagccgtag caatccggtc
     1381 ccttcgctgt aggcgataac ggaaacctgc cccccgatgc gcagctgtct ccgcaaagcc
     1441 tcatcctgcc tggtgtcaaa gccaatgatg accgggaagc gctgtgactg ccgcaaccag
     1501 tcgcggtcgt tctggatcgc cggcagggtc cccgcgggct cgcttcgacc gccacttaca
     1561 cccagaccga tactccgaac ttccccgcga aacacccgtc ccggcagcac gtcgaacaga
     1621 atctcgaccg gcgtgcccac ttccacatga cccaggttat tctcggtgaa ctcagcattg
     1681 acccagacgt cgtgaatcgc aatcagcgtc agcaccgggc tgccggtgcc ggcaaaatgg
     1741 ccaacgtcgg acttaagatc cgtgattacg cccctcgatg gtgccttgac taaggtgttg
     1801 gcgaggtcca gctctgcctt cgccaccgcg gtcagtgcgg tgttgattat cgtattgttt
     1861 gccgggtcgt cgccgcccat ctgctcgatg gctcgctgga tgtccgcttc ggcagcggtt
     1921 acctgtgcct gtgccgcttc gtggcttgcc tgggccactt ccaacagacg caccgagatg
     1981 gtgccgggat cctctttgta cagccgcgcg agccgcgtgg catcctgccg tgccttcctt
     2041 tcattggctc gcgcagcaaa caggttggca cgtgccgcgt tgacggctgc gctgccggcg
     2101 cccacctgcc tgccggcgtt ctcgaagtcg gaccgggccc ggttcagtgc gatctggtat
     2161 tgcgaggagt cgatctcgaa cagcggctga ccttcctcca cttcctggtt gttcgtgacc
     2221 catacgcgtt tcaccgttcc cgcgacctcc ggagcgacgc cgatgatgta tccctcgatc
     2281 ctcgcctgcg tcgtgtaggg ggtgtagcga tcggcgagca cgtaccaggt gaggctcagg
     2341 acgatgacca acgccacgcc gatggcccct ttcctgacgg agcgactcgc ctcttccttc
     2401 tgcggttggc tgtcctgcac gtcggtgccc tcttcgccac cgtcgtgcgg agcctcgtca
     2461 acgatcacct tgtcctcggc cggcctgtca ctcattgtgt gttcctggtt cacccgatgg
     2521 gccgtcgtct ctcggcaaag gctcgctcaa caggtcaccc cagttcgtgc ggctacgcat
     2581 ttcgtcacgt atggattccg gaaccatttg ttccaccgac atgtctgtcc agccgccgcc
     2641 tatcgacctg tagagcgcga tgatcgcgct gatgtgagta ctccgaatga tcaactcctg
     2701 gtccgcctgg gcgaacagcg ctcgctgggc gtccagcact cgttgaaagt ccgcgtagcc
     2761 ttcctgatag cgcgtattcg caagtgcgag cgagcgctcc gcggctctga gcgaatcggc
     2821 gagtacccgt tgctgttcgc ccgtcttcac cacgctgatc gcggcatcgt caatctcccg
     2881 cgctgcctgc agcacgctct cctggaaaat ttcgatcgac tgctgcaagc gggcatcctg
     2941 caaccgcaca ttgtttcgaa tccggccgta atcgaagacg ttccaggaga gcgcagggcc
     3001 gacgacgaaa ctgctggtgt cggagctggc atccagcgaa tccgctgacc agccgatgct
     3061 gccgagaaga gtgatggcag ggtagtagtc ggctttggcc acgccgatct gtgccgactg
     3121 tgccgcgatc tgccatgccg ccgtgcggat gtccggccgg cgaagcagca actgggccgg
     3181 aatcccctga atggctaccg ggtctacggc cggcagctcc cctttgaccg actggagttc
     3241 cggtacgtcg cccggcgttc gacccaggag agcggcaagg gcgttctgca gcttgatcag
     3301 cgttgcttcc agctccggta ttgtggacaa cgtggcgagg tactgagtct tggcctgctg
     3361 caagtccagc tccgagccct gaccagcctc gtaaatcttt tcggtgattt cgaaactgcg
     3421 ctgctgtatg tccgcattcc gctgtgcgat gtcgattcgc agcaatgttg tccagtaagc
     3481 aaagtacagg tccgccacct gtgcgctcag cagtacctgc gcgtcctgtt ggccggtgat
     3541 ggacgcaaaa tacgcggcgt tggcagattc gatgccgcga cgaaatcgtc cccagaagtc
     3601 cagttcccag ccgatgtcga ag
//
LOCUS       DASUOM010000238        13779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1776642,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000238 DASUOM010000000
VERSION     DASUOM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776642"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..794
                     /gene="pstB"
                     /locus_tag="VFY27_04930"
     CDS             6..794
                     /gene="pstB"
                     /locus_tag="VFY27_04930"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5764890.1"
                     /translation="MGKTVGKVRSDNPFMIADNVNVHYGDLHAIKNVTLEIGKQEVIA
                     LIGPSGCGKTTFLRCLNRMNDSIESARVTGRITLDGQDIYAKNMDPVALRARVGIVFQ
                     KPNPFPKSIYDNVAYGPRIHGLAADRAELDEIVRSSLERAGLLKEVKDRMEAPGTSLS
                     GGQQQRLCIARTIAVNPEVILMDEPCSALDPIATARVEDLIDELSENYAIVIVTHSMQ
                     QAARVSKRVAYFHLGMLVEVDDTDRIFTNPQHKLTENYITGRFG"
     gene            997..1473
                     /locus_tag="VFY27_04935"
     CDS             997..1473
                     /locus_tag="VFY27_04935"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5764891.1"
                     /translation="MERIEKSVQVDCPVDVVYNQWTQFEDFPQFMAGVKEIRQIDDTH
                     LRWHAEVWGKDKEWEAEITEQVPDKHIAWRSISGDAPNAGEVRFEPVSADKTRVHLTM
                     EYEPQGAIENIGDALGAMDRRVQSTVDDFKEFIESRNRETGAWRGEVHGGQEKPRH"
     gene            complement(1561..2004)
                     /locus_tag="VFY27_04940"
     CDS             complement(1561..2004)
                     /locus_tag="VFY27_04940"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEX5764892.1"
                     /translation="MTPLELEQYLHRHIPLSLAMQVSVLEVQPQHVVVAAPLEPNINH
                     RDTVFGGSATAVAILAAWSLVHTRLLDAGLPGRLVIQRNIMTFSVPMTGAFTAQASLG
                     DDGQWEKFIRTLARRRRARISVACELTCAGEEAGRFEGDFVALTT"
     gene            complement(2048..2302)
                     /locus_tag="VFY27_04945"
     CDS             complement(2048..2302)
                     /locus_tag="VFY27_04945"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX5764893.1"
                     /translation="MPNPSTRTKSAGGTKKTRQTKATPKRRASSPTEKVSLSPALREE
                     MIREAAYYRAEKRGFQGGDPLADWLSSEEEVNTALSQGVH"
     gene            2701..4206
                     /locus_tag="VFY27_04950"
     CDS             2701..4206
                     /locus_tag="VFY27_04950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5764894.1"
                     /translation="MTAIWNLAGLESRRYAAMGKDLEVEVAIVGGGVTGLSAAIRLLE
                     AGRKVAVLEALQVGQGSSGNSTGNLYGTLSQGLRSVRRKWGDDALRQVGTTRSAAVDA
                     IESRIRHYGIECRFERRPLYFCLPSRDEKQQQDLADEYEASLIAGLRAELVDHAPGMP
                     VPVKLALLVENQAQLNPLRYAQELAKVVASQGGLVFEDTPVIDVDASEGRVSTANGVV
                     TAKHIVFATHTPKGINLVQAEMEPSREYGVAAPLKASPGPEGIFWLLDQFHSVRTYRH
                     EDRTYLVVIGEKHPTGQDESGDAHYDNLRQYARQHFNLEEFSHGWSAQQYKSADQLPY
                     IGRSAHDNVYVGTGYGADGLTWGEVAATIITDLVMDRETEASRLFNPRRFTPVKSAKS
                     WAALNAKVAKHLTTDYLTLDKIEDFEQVRPSEGKVVSAHGDKVAVYRSPDNVLSVLSA
                     VCPHMKCMVKWNPADTSWDCPCHGSRFKPEDGSVIEGPAYGPLTRLELRDS"
     gene            complement(4234..4455)
                     /locus_tag="VFY27_04955"
     CDS             complement(4234..4455)
                     /locus_tag="VFY27_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5764895.1"
                     /translation="MNHQLPGKRYGCAFQRRSARVTEDEVRASIRSAGLLAMQEVEAV
                     VLETDGSFSVVRQGGGAGGSSLSGISRPA"
     gene            complement(4456..5673)
                     /locus_tag="VFY27_04960"
     CDS             complement(4456..5673)
                     /locus_tag="VFY27_04960"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX5764896.1"
                     /translation="MGEASWLLTFNAGSSSLKVGVFEYRDGVRSRLRFAVRDIGRESS
                     TLSTGTAAEDLGHIDNVEAAATLVLDRLENGIGGTTVDRRSVAATGHRIVHGGEHYAS
                     AVRVNRDVFETLDSLNHLAPLHNPPALAIMKIVRERFPGVALVADFDTAFFHDLPEAA
                     RVYAVPSGLAERHAIRRYGFHGLAHQFMSRQLELLSPDGKHPDRMVTLQLGQGCSAAA
                     LRDGRPVETSMGFTPMEGLVMGTRSGDVDAGVLLYLAQQGYPWDELEDVLNRKSGLLG
                     LSGESDDTRALLELEARQHPGARLALQVFCHRILKYLGAYAAVLGGIDAIAFGGGIGE
                     NAPVIRSRVCSGLGWLGVQLDEDANAGCTGKGGRISTDESSIDVHVVPVDEEAIIAAA
                     TWHILRGADDRAS"
     gene            complement(5689..6879)
                     /gene="tal"
                     /locus_tag="VFY27_04965"
     CDS             complement(5689..6879)
                     /gene="tal"
                     /locus_tag="VFY27_04965"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5764897.1"
                     /translation="MATRGETRRMSPVRKLVDFGQSPWLDFIQRGLLTGGELERMIAD
                     WGLRGLTSNPVIFEKAIAHTDEYDADIEALAREGSSAEEIYETLAIQDVQQAADLLLP
                     VYEESDRSDGFASLEVSPHKANDGGATVAEARRLWAALGRRNVMIKVPATAEGLVALR
                     TLLADGINVNVTLLFSVDRYREVLQSHLEGMEDAIAAGKDPGRIASVASFFLSRVDTL
                     VDARLDQLAAKDDAHSKAAAALRGETAIASARQAYAVFEEFIAGSRFRKISTQGARPQ
                     RLLWASTGTKDPAYSDVRYVEALIGPLTVNTMPLETIKAYDDHGQPELRLTGHADDAA
                     AKLRKLAELGIDLHEVTGQLLQQGIDKFVKPYDSLVGCLERARRAALAAPSIGIDQRA
                     ERGA"
     gene            complement(6881..9334)
                     /locus_tag="VFY27_04970"
     CDS             complement(6881..9334)
                     /locus_tag="VFY27_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX5764898.1"
                     /translation="MARSAPVSKEQRHARNDGRDQAAAGLRSSELERAELLKRVDAYW
                     RAANYLAVGQIYLMDNPLLKEPLSLEHVKPRLLGHWGTTPGLNLIYAHLNRVIIERDL
                     NMIFLTGPGHGGPALAANTWLEGTYSEMYPEVAMDAEGMRRLFKQFSFPGGIPSHCAP
                     EIPGSIHEGGELGYVIAHSFGAAFDNPDLVVACIIGDGEAETGPLAASWHSNKFLNPA
                     TDGAVLPVLHLNGWKIANPSFFARLPHEELEHLIRGYGYEPTFVEGDDPHAVHEEMAA
                     ALDRLLDRIGAIQQEARNGGTNNRPVWPMLVLRTPKGWTGPEEVDDKLVEGTWRSHQV
                     PLSGLKNNPDHLRQLEAWLQSYEPGKLFTAEGCLVPELQELAPKGARRMGANPHANGG
                     VLLKDLRLPDFRDYGVEVKKPGGTKAESTRVLGSFLRDVMKKNLDKANFLVFGPDETA
                     SNRLAALYEVTGKRFLGPYLPVDENLAADGRVIEVLSEHLCQGWLEGYLLTGRHGLFS
                     CYEAFIHIIDSMFNQHAKWLKVSKEIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGF
                     IDHVVNKKADIIRVYLPPDANTLLCVADQCLRSRDFVNVIVAGKQMQPQYLDMDAAIK
                     HCAAGIGIWPWASSDQDAEPDVVMACCGDVPTMETLAAVSILREQLPDLKVRVVNVVD
                     LMTLQPGTEHPHGLSDHDFDAIFTRDKPIIFAYHGYPWLIHRLTYRRRNHDNLHVRGY
                     KEEGTTTTPFDMAVLNDIDRFHLVGDVIDRVPKLGSVAAYRKQYVRDKLIEHKQYIHE
                     HGEDMPEVQQWAWPGEQED"
     gene            complement(9520..10470)
                     /locus_tag="VFY27_04975"
     CDS             complement(9520..10470)
                     /locus_tag="VFY27_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5764899.1"
                     /translation="MIYLYLAAGLLLLLVGGELLVRGAVATATRAGVSPLLIGLTLVG
                     FGTSTPELLTSVEAAMVGSPGIAVGNVIGSNIANILLILGVAALICPLKVSRGAFYRD
                     GTVVTIAALCGVAVCLTGRLQPVGGIFLLMLLAAYIVYSYRAERVHASVTDTPVHPAD
                     AVPGSIDITLPMSIVFVAGGLALTMLGAHLLVTSALAIGTRLGISETVLGLTVVAVGT
                     SLPELATSVLAAVRRQPDIAFGNIVGSNIYNILGILGVTAIVEPIPVPPEIARTDAWI
                     MLGATLMLLVFSISGWRITRFEGAVFLAAYGAYLFLVGFS"
     gene            complement(10489..11325)
                     /locus_tag="VFY27_04980"
     CDS             complement(10489..11325)
                     /locus_tag="VFY27_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX5764900.1"
                     /translation="MTIPKLATMMSSEHVRELREAREILQHPSLVIRLSSMVGTRVEN
                     LTKQLGKRLPEEMTEVISRSSNKAIESAFHAAIRTIQKGNLSAPHNRLHQAAVVTTGS
                     VAGFLGLHALLVELPITTLIMFRSIADIARANGESLADPETQLNCLTVFALGSPKSDA
                     DEGAETSYYGVRYALSRLTTDALQYITAHGTTGRSAPVMVRFVSAIASRFGVVVSQKA
                     AAQAVPAIGAIGGAVTNAVFMSHFQDMARAHFTIRRLERIYGKDAVQELYAALADDLQ
                     ES"
     gene            complement(11449..>13779)
                     /locus_tag="VFY27_04985"
     CDS             complement(11449..>13779)
                     /locus_tag="VFY27_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764901.1"
                     /translation="RAYAFGTINWADREEILQGDRQFSEVALDWDGDSFEPVGSSTIR
                     QGRWDAIASNAPSQAALDAYFLAQDPAYAAGTALPGHNFGFNPDGSIFGTAPVINFTG
                     DTNEPLQPVNPAEYTYNFAPPNYLQLPLERTSFFGRAGFNLSEDTEIFTQVMWADYKA
                     DQALAPTPMTGVYVRPDNPNIAPDLAALLASRPDPTAPFAFSKRLEEAGPRIASNNYE
                     VVQLLAGMTGSVPFGFAEDWTWDVYGSWGNVENTELQQGNVSRTALEELTLADDHGAS
                     LCGEAINPFGISSVQPACAEYITRGAINNTTVRQIVGEAFVTGPLFEMPAGEVQAAAG
                     VMYKQDEVAFLPDPTLTATSIDPLFGNERTDIVGFNAQDVVRGDTNSREIYVESSFPL
                     LGDMPAIQALDLTLGYRFADHSTAGTMNSYKAEGIWDINEMFTVRGGYQRAVRAPNID
                     ELYTPATINFPSVGLGDPCSNDFNDPEGNVLGAQDDPAARALCVAQGIPESAIDSFIF
                     SNSQFQGLAGGNPDLSEETADTYTLGLVFQPADGPLAGLQASVDYYNIEIEDAIDAIT
                     ADVIVERCFDRRFNPDLTNDNFYCSFFERLPGTNTITSALEVDTNIAAFEVEGVDLQV
                     DYSMEAGPGELRGKWVSTYLMGFKDATVQGEEMTENAGTASSDFDALPEWKWMASVGY
                     AWAGFDGDLRWRHIDEMTDFSFPAFKLDAVDYLDLTIGYSFTDALEGLRLRAGVTNLT
                     DEDPIIYPSQQQANTDPSTYDVLGRRYFVSATYSIE"
BASE COUNT         2708 a         4172 c         4244 g         2655 t
ORIGIN      
        1 agctgatggg caagacggta ggcaaggttc gctccgacaa cccgttcatg atcgccgaca
       61 atgtcaacgt ccattacggc gacctgcacg cgatcaagaa cgtgacgctg gagatcggca
      121 agcaggaggt catagcgctg atcggcccat ccggttgcgg aaagacgact ttcctgcgct
      181 gtctcaatcg catgaacgat tcgatcgaat cggcgcgggt gaccggccgc atcaccctcg
      241 acggccagga catctacgcg aagaacatgg acccggtagc gctgcgggct cgtgtcggca
      301 tcgtgttcca gaagccgaac cctttcccga agagcatcta cgacaacgtc gcttacgggc
      361 cgcgcattca cggcctcgcc gccgacaggg cggagctcga cgagatcgtg cgctcgagcc
      421 tggagcgcgc ggggctgctg aaggaagtga aggaccggat ggaagcgccc ggcacgagtc
      481 tttccggcgg acaacaacaa cgactttgca tcgctcgcac gatcgcggtg aatcccgagg
      541 tcatcctgat ggatgaaccc tgctccgcgc tcgatcctat cgccacggct cgggtagaag
      601 acctcatcga cgagttgtca gagaactacg caatcgtgat cgtgactcac tcgatgcagc
      661 aggccgcgcg cgtttccaag cgggtcgcgt actttcacct cggcatgctc gtcgaggtgg
      721 atgatacgga caggattttc accaacccgc agcacaagct cactgaaaac tacatcaccg
      781 gccggttcgg ataatcgttc gcctccggtg taaggaatac aagcgttggt aacgcttaca
      841 cccgcggggt aaggctagag tgccccagtc gctccaaggg tgtctcggcg tagccggaca
      901 cgaccaagcc cgctctctct actccgctac ggcacaaaac ttgtatgttt cctgcacccg
      961 aaaacgattt aactgttcca taaaaggaga tcagctgtgg aaagaatcga gaaatcagtg
     1021 caggtagatt gcccggtcga cgtcgtctac aaccagtgga cgcaattcga agattttccg
     1081 cagttcatgg cgggcgtaaa ggagatcagg cagatcgacg acacgcacct gcgctggcac
     1141 gcggaggttt ggggcaagga caaggagtgg gaagcggaga ttaccgagca ggtccccgac
     1201 aagcacatcg catggcgcag catttcgggc gatgcgccca atgccggcga ggtgcgtttc
     1261 gagccggtgt cggcagacaa gacgcgcgtg cacctgacga tggagtacga accgcaaggc
     1321 gccatcgaga acataggcga tgcgcttggc gcgatggatc ggcgcgtaca gagtaccgtc
     1381 gatgacttca aggaattcat cgagagtcgc aatcgcgaga cgggtgcctg gcgcggcgaa
     1441 gtgcacggcg ggcaagagaa gccacggcac tgaaccaggg tcatcatcca accgtagcgg
     1501 ggcggccgtg gactggcgcc gtcccgcgac aggacggaaa aaaccgcact accgaagagg
     1561 tcaggtagtc aaggcaacaa aatcaccctc gaaacggccc gcttcttcgc ccgcgcaagt
     1621 caattcgcac gcgacagata tcctggctct tcttctgcgc gccagcgtcc ggatgaattt
     1681 ttcccactgc ccgtcatcgc caagcgatgc ctgcgccgtg aatgcgccag tcatcggtac
     1741 tgaaaaggtc atgatgttgc gctgaatgac cagccggccg ggcaagccgg cgtccaggag
     1801 ccgggtatgt accagcgacc atgctgccag gatggcgacg gccgtcgcgc ttcctccgaa
     1861 tacggtatcc ctgtggttta tattcggctc cagtggagcg gccacaacaa cgtgctgcgg
     1921 ttgcacctcc agcactgata cctgcatcgc cagggaaagc gggatatgcc gatgcaggta
     1981 ttgctccagt tcaagcggtg tcacaagcag aattccgcga tctgcccgtt accgccgctc
     2041 aggctctcta gtggacaccc tgtgacaatg cagtattcac ttcttcctcg ctggacaacc
     2101 agtcggcgag cgggtcccca ccctggaagc cgcgcttttc ggcacggtaa taggcggcct
     2161 cccggatcat ctcctcgcgc agcgcaggcg acaggctcac cttctcggtt ggcgaacttg
     2221 cgcgacgttt cggcgtcgcc tttgtctgcc tggtcttttt tgttccgccg gcggattttg
     2281 tccgggtact cggattgggc attacgtaac tcctttgttg agatgggttg gaggcgctag
     2341 cgtaatgcat tttgccgtgc gtctgttaca gattatcaac tcttcaccgg atttcgttgt
     2401 cgccgccgtc agcgacgatg gtgaccgcga cggccttgac cgaccccgtc atcaaaatca
     2461 aatcagggtg tcactttccc cgtaagctgc tgcgaggatt cactacggag cgtggtaaaa
     2521 tttgcacaag ccagaacgaa acgtcacaga ggcggtgagc ttcctggggg ttgacgcaac
     2581 ggcggaaatc acccgcttcc tgacatggag ccgccttgga cccgaggcgg cgctgcaatt
     2641 tggagtatcc tgaaagcgga tgacagctcg gcgccgcatg tgtcatcggg agtacatgca
     2701 atgactgcca tctggaacct cgcgggcctg gagagccggc gctatgctgc catgggcaag
     2761 gacctggaag tcgaggtcgc aatcgtcggc ggtggcgtta ccgggctgtc cgccgcgatc
     2821 cgactgctgg aggcgggccg taaggtcgcg gtgctggagg ccttgcaggt cgggcagggc
     2881 agcagcggca attcgaccgg caacctgtac ggcaccttgt cacagggtct ccgcagtgtg
     2941 cgccgcaaat ggggagatga cgcgctgcgg caggtgggga caacccgaag cgctgccgtt
     3001 gacgctatcg aaagccgcat ccggcattac gggatcgaat gccggttcga acggcgtcct
     3061 ctgtatttct gcctgccgag ccgtgatgaa aagcagcagc aggacctcgc agatgaatac
     3121 gaagcttcgc tcatcgccgg gctgcgagcg gaactcgtcg atcacgcgcc ggggatgccg
     3181 gtgccggtaa agcttgcgct gctcgtcgag aaccaggcgc aattgaaccc cctgcgctac
     3241 gcgcaggagt tggcgaaggt ggttgcgagc cagggagggc tggttttcga ggacacgccc
     3301 gtcatcgacg tcgacgctag tgagggcagg gtctcgaccg cgaacggggt cgtcaccgcg
     3361 aagcacattg ttttcgctac ccacacgcca aagggcatca accttgtaca agcggaaatg
     3421 gaaccatcgc gcgagtacgg agtggcggca ccgctcaaag caagcccagg tccggagggg
     3481 attttctggc tgctggacca gttccattcg gtgcggacgt accgtcacga ggatcggacg
     3541 tacctggtgg tgatcgggga gaagcacccg acgggccagg acgagtccgg cgacgcgcac
     3601 tacgacaacc tccggcaata cgcgcgccag cacttcaacc tggaggagtt ttcgcacggc
     3661 tggtcggcgc agcagtacaa gagcgcggac cagttgcctt acatcggcag gagcgcgcac
     3721 gacaacgtct atgtgggaac gggttacggc gcagacggcc tgacgtgggg cgaggtcgcg
     3781 gcaactatca ttaccgacct ggtaatggac agggaaacgg aagcctcccg acttttcaat
     3841 ccccggcgct ttacgccggt gaagtccgca aaaagctggg cagcgctcaa cgcgaaagtt
     3901 gcgaagcatc tcaccacgga ctacttgacg ctcgacaaga tcgaggactt cgagcaggtc
     3961 cgcccaagcg aggggaaagt tgtcagcgca catggcgaca aggtcgccgt ataccgctcg
     4021 ccggataacg tgctgtcggt gctgtcggcg gtttgcccgc acatgaagtg catggtcaag
     4081 tggaaccccg ccgataccag ttgggactgc ccatgccacg gaagccgatt caaacctgaa
     4141 gatggcagtg tcattgaagg tcctgcctac ggtccgctaa cgcgactgga gctgcgggat
     4201 tcgtgaatcg tcacaagcac ctcgatgcgg aggttacgct ggtcggctga tgccggagag
     4261 gctggagcct ccagcgccac cgccttgccg cacgacgctg aacgagccgt cggtttccaa
     4321 tacgacggcc tcgacttcct gcattgcgag caaacccgcg gagcgtatgg atgcccttac
     4381 ctcgtcttcg gttaccctcg ccgaacgccg ttgaaaagcg catccatatc gtttccctgg
     4441 gagttgatgg ttgattcagc tggccctgtc gtcggcgccc cgcaggatgt gccatgtcgc
     4501 agccgcaatg atggcttcct cgtcgacagg aacgacgtgc acatcgatgc tggattcgtc
     4561 ggtcgagatc ctgccgcctt tcccggtgca ccctgcgttc gcgtcctcat cgagttgcac
     4621 accgagccag cctaatccgg aacagacgcg tgaccgtatg acgggcgcgt tctcgccgat
     4681 gccgccgccg aaggcaatcg cgtcaatccc gccgaggacg gccgcatagg cgccgagata
     4741 cttcaatatg cgatggcaga agacctgcag tgcgagccgt gcccccggat gttgcctcgc
     4801 ctccagttcc agcagcgcac gggtatcgtc ggattcgccg gacaagccca gcaagccgga
     4861 tttcctgttg agcacatcct ccagttcgtc ccacggataa ccctgctgcg ccaggtaaag
     4921 cagcaccccc gcgtcgacgt cgcccgagcg cgttcccatt acgagtcctt ccatgggagt
     4981 gaagcccatg ctggtctcta ccggcctccc atcacgaagt gctgcagcag aacagccttg
     5041 cccgagctgc agggtcacca tgcggtcagg atgcttgccg tcgggtgaca gtaactcgag
     5101 ctgcctggac atgaactggt gggcaagacc gtggaagccg taacggcgga tagcatgacg
     5161 ttcggccagt ccggacggaa ccgcgtaaac acgcgctgct tccggcaggt cgtggaaaaa
     5221 ggcggtgtcg aagtccgcca ctaacgcgac gcccgggaac cgctcacgca cgattttcat
     5281 gatcgcgagt gccggggggt tgtgcagagg ggcgaggtga ttcagcgagt cgagcgtctc
     5341 gaaaacatcg cgattcaccc gcaccgcgct agcgtagtgc tctcctccgt gaacaatcct
     5401 gtggccggtg gcagcaacac tccggcgatc gacggttgtt ccgccgatgc cgttctccag
     5461 tcggtcgagc accagcgtcg ccgcggcctc gacattgtca atgtggccga ggtcttcggc
     5521 tgccgtgccg gtgctcaggg tcgagctttc gcgcccaata tcgcgtaccg cgaagcgaag
     5581 gcgcgaacgg acgccgtcgc ggtattcgaa cacgccgacc ttgagcgatg agctgcccgc
     5641 attgaacgtc aacagccagc tggcctcacc catgtcgatg cgggcggatc aggcgccgcg
     5701 ttccgcgcgc tggtcgatcc cgatggaagg agccgcgagg gcggcgcggc gggccctctc
     5761 caggcatccg accagtgaat cgtatggctt gacgaacttg tcgatgccct gctgcagcaa
     5821 ctggcctgtc acctcatgaa ggtcgatgcc gagctccgcg agcttccgga gtttcgccgc
     5881 ggcatcgtct gcgtggccgg tcaggcgcag ttccggctgg ccgtggtcgt cataagcctt
     5941 gattgtttcg agtggcattg tgttgaccgt cagcggccct atcagcgctt ccacgtacct
     6001 gacgtcgctg taagccggat ctttcgtgcc ggtgctcgcc cacagcaacc gttgcggacg
     6061 cgcgccctgc gtgctgatct tgcggaatcg tgaacccgct atgaattctt cgaacaccgc
     6121 atacgcctgc cgcgcgctcg cgatagccgt ctcgccgcgc aaagcggccg ctgccttgct
     6181 gtgcgcatcg tctttcgctg ccagctggtc cagccgcgca tcgaccagtg tgtccacgcg
     6241 gctcaggaag aagctggcga cagaagctat ccggccggga tccttaccgg cggcgattgc
     6301 atcctccatg ccctcgagat gagactgcag tacctcgcgg tagcgatcca ccgagaacaa
     6361 tagcgtcaca ttgacgttga tcccgtcggc cagcagcgtg cgcagcgcaa ccagcccttc
     6421 ggccgtggca ggcaccttga tcatcacgtt gcgccgtccc agcgccgccc acagacgcct
     6481 ggcttcggca accgtcgcac cgccgtcgtt cgctttgtga ggagatactt cgaggctggc
     6541 aaagccatcc gacctgtcgc tctcctcgta aaccggcagc agcaggtccg ccgcctgttg
     6601 cacgtcctgg atcgccaggg tttcgtaaat ctcctcggcg ctggaaccct ccctggcgag
     6661 cgcttcgata tctgcgtcgt actcgtcagt gtgagcgata gctttttcga agatgacggg
     6721 gttcgaggtg agcccgcgca gtccccaatc cgcaatcatg cgttccagtt cacctcccgt
     6781 gagtagcccg cgctggatga aatcgagcca cgggctctgg ccgaaatcca ccaatttcct
     6841 caccgggctc atgcgacggg tctcgcccct ggttgccatc tcaatcctcc tgttcgccgg
     6901 gccacgccca ctgctgcact tccggcatgt cctcgccgtg ctcgtggatg tattgcttgt
     6961 gctcgatcag cttgtcccgg acgtactgtt tgcggtaggc ggcgaccgaa cccagtttcg
     7021 gcacccggtc gattacatcg cccacgaggt ggaagcggtc gatgtcgttc agcacggcca
     7081 tatcgaacgg cgtggtagtc gttccctctt ccttgtagcc gcggacatgc aggttgtcgt
     7141 gattcctgcg ccggtaagtc aggcggtgaa tcagccacgg atacccgtga taggcgaaga
     7201 taatcggctt gtcacgcgtg aagatcgcgt cgaagtcgtg atctgacagc ccgtgaggat
     7261 gctccgttcc cggctgaaga gtcatcaggt cgacgacgtt gaccacccga accttcaggt
     7321 ccggcagttg ttcgcgcagg atggacaccg ccgccagtgt ttccatggtg ggtacgtcac
     7381 cgcaacatgc catgaccacg tccggttcgg cgtcctggtc gctgctcgcc cagggccaga
     7441 tgccgatgcc cgcggcgcaa tgtttgattg cagcatccat gtcaaggtat tgcggctgca
     7501 tctgcttgcc ggcgacgatc acgttgacga agtctcggga gcgcaggcac tggtccgcaa
     7561 cacacagcag agtgttcgca tccggcggca ggtaaacccg gatgatgtcc gccttcttgt
     7621 tgaccacgtg gtcgatgaag ccgggatctt ggtggctgaa gccgttgtgg tcctggcgcc
     7681 atacgtgcga ggtcaggagg tagttgagcg atgcaatcgg ccggcgccac gggatttcct
     7741 tgctgacctt gagccacttt gcatgctggt tgaacatgga atcaatgatg tggataaacg
     7801 cttcgtagca ggagaacaag ccgtgccggc cggtcagcaa gtagccttcc agccatccct
     7861 ggcacaggtg ctccgaaagc acttctatga cgcggccgtc ggcggcgaga ttttcatcca
     7921 cgggcaggta aggaccgaga aagcgcttcc ccgttacctc gtaaagagca gcgagcctgt
     7981 tggacgccgt ctcgtcgggc ccgaacacca ggaagttcgc cttgtccagg ttcttcttca
     8041 tgacatcccg gagaaaactc cccaggaccc gggttgattc cgcttttgtc ccgccgggct
     8101 tcttcacctc gacgccatag tcgcggaagt ccggcagccg caggtccttg agcagtacac
     8161 ccccgttcgc gtgagggttc gcgcccatgc ggcgcgcgcc tttcggcgca agttcctgca
     8221 actcgggaac aaggcaacct tccgcggtga agagcttccc gggttcgtag ctctgcagcc
     8281 acgcctcaag ctgacgaaga tgatccgggt tgttcttgag ccccgaaagc ggcacctggt
     8341 gcgagcgcca cgtcccttcg acaagcttat cgtcaacttc ctccggtcca gtccacccct
     8401 tcggcgtgcg caaaacgagc atcggccata cgggccggtt gttcgtgccg ccgttccgag
     8461 cctcctgctg gatagcaccg atgcgatcca gcagacgatc cagcgctgca gccatctctt
     8521 cgtgcactgc gtgcgggtcg tcgccttcta cgaaggtcgg ctcatagccg tagccgcgga
     8581 tcaaatgctc cagttcttca tgcggaaggc gcgcgaaaaa gctcgggttg gcgatcttcc
     8641 agccatttag atgcaggaca ggcagcactg cgccgtccgt cgccggatta agaaacttgt
     8701 tggagtgcca gctcgcggcg agcggtcctg tttcagcctc accgtcaccg atgatgcagg
     8761 ccacgacgag gtccgggttg tcgaacgcag caccgaagga atgtgctatc acgtagccca
     8821 gttcaccgcc ttcatgaatg gacccgggaa tttccggcgc gcagtggctg ggaatcccgc
     8881 cgggaaacga aaactgcttg aagaggcggc gcatgccttc cgcgtccatc gccacttcgg
     8941 ggtacatctc gctgtacgta ccttcgagcc atgtattcgc ggccagcgcg gggcccccgt
     9001 gacctggtcc cgtgaggaag atcatgttca ggtcgcgctc gatgatcacg cgattcaggt
     9061 gcgcgtagat caggttgagg cccggtgtgg ttccccagtg tccgagaagg cgcggtttca
     9121 cgtgctcgag cgacagcggc tccttcagca ggggattatc catcaggtaa atctggccga
     9181 ccgcgaggta atttgcggcg cgccagtaag catcgacacg tttcaggagc tccgcgcgtt
     9241 ccaattcgga tgaccggagc cctgctgccg cctggtcgcg gccgtcgttt ctcgcgtgcc
     9301 tttgttcctt cgatacgggt gcgctacggg ccatagttcc gtttcctcgg aggtgcttgt
     9361 gaaaaggtgt aagtgtcggc gccggtgatg tcggcgaaag ggcgttgaag cgcgcggact
     9421 cgcggggtgc tgccaggcgc gtgagtgatt atatcgtctt gatcgggaac cgtctgttcc
     9481 gccctgttgc gttcctcaag acgagcagag ggctcaacct caggaaaacc ccacgaggaa
     9541 caggtaagcc ccgtaggcag cgaggaaaac agcgccctcg aatcgggtga tccgccagcc
     9601 gctgatggag aacacgagca gcatcagggt cgcgcccagc attatccagg catcggtgcg
     9661 cgcgatttcc gggggaaccg ggatcggctc gactatcgca gtgacgccga ggattccgag
     9721 aatattgtaa atgttgctgc cgacgatgtt gccgaatgca atgtccggct gccggcgaac
     9781 ggcagcgagc acggaggtcg cgagttccgg gagagaagta cccaccgcaa cgacggtcag
     9841 ccccagcacg gtctcggaaa tgccgagacg agtaccgatc gcgagtgcgc tggtcacgag
     9901 cagatgtgcg cccagcatgg tcagggcaag tccgcctgca acgaatacga tcgacatcgg
     9961 cagcgtgatg tctatcgatc ccggaaccgc gtcggcagga tggaccggcg tgtcagtgac
    10021 agaggcatga actctttcgg cgcggtagct gtagacgata taggcggcca gcagcatgag
    10081 caggaagatg ccgcccacag gctgcaaacg cccggtcagg caaacggcga cgccgcagag
    10141 ggcagcaatc gtaaccaccg ttccgtcgcg gtagaaggca ccgcgcgaga ccttgagcgg
    10201 gcaaatcagc gctgcaaccc cgaggatgag caatatgttc gctatgttgc tgccgatgac
    10261 attgccgacc gctatgccgg gcgagcccac catcgcagct tcgacgctcg tcagtagttc
    10321 cggcgtcgag gtgccaaagc cgaccagcgt cagcccgata aggagaggcg atacaccggc
    10381 gcgcgttgct gtcgcgactg cgcctcgtac gagaagttcg ccgccgacga ggaggaggag
    10441 caggcctgct gccaggtaga gatagatcac gatctacgac ggcggccgtc aggattcctg
    10501 caggtcatcc gccagtgcgg cataaagttc ctggactgcg tccttgccat agattctctc
    10561 cagccgcctg atcgtaaaat gcgccctggc catatcctgg aaatgagaca tgaacacggc
    10621 gttcgttacc gcaccaccga ttgcgcctat cgccggcacg gcctgcgctg cggccttctg
    10681 cgaaacgacg acgccgaacc gggacgcgat cgcggaaaca aaacgtacca tgacgggagc
    10741 agaccggccg gtcgttccgt gcgccgtgat gtactggagc gcatcggtgg tgagccgcga
    10801 aagcgcatag cggacgccgt aatagcttgt ctcggcaccc tcgtccgcgt ccgatttcgg
    10861 gctgcccagg gcaaaaaccg tcaggcaatt gagctgggtc tccgggtcgg cgagcgattc
    10921 gccattcgct ctcgcgatat cggcaatcga ccggaacatg atcaatgtgg ttatcggcag
    10981 ttcaacaagc agtgcatgca gcccaaggaa gccggccaca ctgcccgtgg taacgacggc
    11041 tgcctggtgc aggcgattat gcggggcgga aaggtttccc ttctggattg tcctgatggc
    11101 agcatggaat gccgattcga ttgccttgtt gctcgaccgc gatatgacct ccgtcatctc
    11161 ttcgggaagg cgcttcccga gttgcttcgt caggttttcg acccgcgtgc cgaccatcga
    11221 ggacaggcgg atgaccagcg agggatgctg caggatttcc cgggcctctc gcaattcccg
    11281 cacatgctcg gaggacatca tggtcgcaag tttcggtata gtcatctcag gagtagctga
    11341 agaactgtat tgcagtatta cgccggttgc ctgcccgggc aagacttgga ctctgcaaaa
    11401 aaacggcggg ccgaagcccg ccgccgtagt tacgccgtct tccgcggttt actcgatcga
    11461 ataggttgcc gaaacaaagt agcggcgacc aagaacgtca taggttgacg gatcggtatt
    11521 cgcttgttgt tgcgacggat agatgatcgg atcctcgtcc gtcaggtttg tgacgcctgc
    11581 tcgcaaacgc aatccctcca aagcgtcggt aaagctataa ccgatcgtga gatcgagata
    11641 atcgacggcg tcgagcttga acgccgggaa cgagaagtcc gtcatctcat cgatgtgcct
    11701 ccagcgaagg tcgccgtcga acccagccca cgcatagccg accgatgcca tccacttcca
    11761 ctccgggagt gcatcgaaat cgctcgatgc ggtgccggca ttctccgtca tctcctcgcc
    11821 ctgtaccgtg gcgtccttga agcccatcag gtaggtggac acccactttc cacgcaactc
    11881 gccgggaccg gcttccatcg agtaatcgac ctgcaggtcg acaccctcga cttcgaacgc
    11941 ggcgatattg gtatccactt cgagagcgct ggtgatggtg ttggtccccg gcagccgctc
    12001 gaagaacgaa cagtagaagt tgtcgttcgt cagatcagga ttgaagcggc ggtcgaaaca
    12061 gcgctccacg atgacatcgg cggtaattgc gtcgatcgca tcctcgattt cgatattgta
    12121 gtagtcgacc gacgcctgca atccggcaag cggaccgtcg gctggctgaa acaccaggcc
    12181 gagggtgtag gtatcagcgg tttcctccga gaggtcgggg ttgccgccgg cgagtccctg
    12241 gaactgggaa ttgctgaaga tgaaagaatc gatcgcgctt tcgggtattc cctgcgcgac
    12301 gcacaatgct cgcgcggcgg ggtcatcctg ggcaccgagg acgttacctt cgggatcgtt
    12361 gaagtcgttc gagcatggat caccaagccc gacggacggg aagttgattg tcgccggcgt
    12421 atacagctcg tcgatattcg gtgcccgtac cgcacgctga tagccgccgc gtacggtgaa
    12481 catctcgttg atatcccaga tgccctcggc cttgtaggag ttcatggtgc cggcggtcga
    12541 atgatccgcg aagcgataac cgagtgtcag gtcgagtgcc tggatcgccg gcatgtcgcc
    12601 cagcagcggg aagctgcttt caacgtagat ttcgcgggag ttggtgtctc cgcgaacgac
    12661 gtcctgggca ttgaagccca cgatgtcggt tcgctcgttg ccgaatagcg ggtcgatgct
    12721 ggtcgccgtc agggtcgggt ccggcaggaa cgcgacttca tcctgcttgt acatgacgcc
    12781 tgctgcagct tgcacctcgc ccgcgggcat ctcaaacagg ggaccggtga cgaatgcttc
    12841 accaacgatc tgccggaccg tcgtgttgtt gatcgctccg cgcgtaatgt attccgcgca
    12901 tgcaggctgg acggaactga taccgaacgg attgatcgcc tcgccacaga gtgaggcgcc
    12961 atggtcatct gccagtgtca gctcctcgag cgcagtccgg gagacgttcc cctgttgcag
    13021 ctcggtgttc tcgacgttgc cccaggaccc ataaacgtcc caggtccagt cctcggcaaa
    13081 gccgaatgga accgatcccg tcatgccggc gaggagctgc acgacctcgt agttgttgct
    13141 ggcaatacgc ggcccagcct cctccagccg cttcgagaac gcgaacggcg ctgtcggatc
    13201 aggacgggac gccagcaacg cggcaaggtc gggcgctatg ttcggattat ccggtctcac
    13261 gtacacgccc gtcatcggcg tcggcgccag tgcctggtcg gccttgtagt cggcccacat
    13321 gacctgcgtg aaaatctcgg tgtcttcgct gagattgaac ccggctcgcc cgaagaacga
    13381 ggtccgctcc agcggcagct gcaggtaatt cggcggggcg aagttatagg tgtactcggc
    13441 cggattgacg ggttgcagcg gctcgtttgt gtcgccggtg aagttgatta cgggcgccgt
    13501 gccgaatatc gaaccgtccg gattgaatcc gaagttatgc ccggggaggg cggtaccggc
    13561 ggcataggcg ggatcctgag cgaggaaata agcatccagg gcagcctgtg acggtgcatt
    13621 gctggcaatc gcatcccagc ggccctggcg gatcgtgctc gaaccgaccg gctcgaagct
    13681 gtccccgtcc cagtcgagag ccacctctga gaactgacga tctccctgca gaatctcttc
    13741 ccggtcggcc cagttaatcg taccgaatgc gtaggcacg
//
LOCUS       DASUOM010000239         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1249147,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000239 DASUOM010000000
VERSION     DASUOM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249147"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..677
                     /locus_tag="VFY27_04990"
     CDS             195..677
                     /locus_tag="VFY27_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008606679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5764902.1"
                     /translation="MTIKVGDRMPSGEFGVMTDKGPGKISSDELFAGKKVVLVSVPGA
                     FTPTCSLNHLPGFVDHADEIMTKGVDTIACMAVNDVFVMNAWGKDRKVGDKVRMLADG
                     NGEYTRKLGLEMDGSGFGLGKRGQRFAMVVDDGVVKHLAVEEPGKFEVSKAEAILARL
                     "
     gene            complement(716..1660)
                     /locus_tag="VFY27_04995"
     CDS             complement(716..1660)
                     /locus_tag="VFY27_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5764903.1"
                     /translation="MSKILVIAEHDGNSINLSTAKCVACASGLPDAEVHVAVFSDAGS
                     EVAAAAARLSNVGKVLQVDQPANKYLLAAVLAPQIAALSEGYSHIFGPSTTFGKDLMP
                     RVAALLGVNQVSDVMNIESSHRFKRPVYAGNAVLTVEAPSGQPVVATVRTASYQAVGN
                     EGGATVEKVPVDAAIPDHTRFVELKAGKSDRPDLQTASTVVSGGRALGGTESFDIIYK
                     LADVLGAAVGASRAAVDAGYVPNEMQVGQTGKIIAPDLYIAVGISGAIQHLTGIKDAG
                     TIVAINKDAEAPIFEVADIGLVGDLFTIVPELTEALRK"
     gene            complement(1660..2409)
                     /locus_tag="VFY27_05000"
     CDS             complement(1660..2409)
                     /locus_tag="VFY27_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5764904.1"
                     /translation="MEKILVGLKRVVDFNVRVRVKSDGTGVETDGVKMSINPFDEIAL
                     EEALRIRERGEASEVVAVSIGSSEAQQQLRTGLAMGADRAILVTTGSPIDQLTVARVF
                     LALVEREKPDLVLLGKQAIDNDNNQTGQMLAALWERPQATFASSVELSGSTARVTREV
                     DTGLETIEIELPGVVTTDLRLNEPRYVKLPDIMKAKKKPLDQLSLEELGVQPAQGLKE
                     ISWEAPPQRSKGAMVNDVAELVAALNAKGLV"
     gene            2527..>3012
                     /locus_tag="VFY27_05005"
     CDS             2527..>3012
                     /locus_tag="VFY27_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5764905.1"
                     /translation="MAYENFLVRPHELHEHLHDDHWRVVDCRFDLANPDKGYSEYLAG
                     HLPGAVYAHLDRDLAAPVTGRTGRHPLPDPEAFALTLGKLGIQRESHVIAYDHGSGAI
                     ASRLWWLLRWMGHPSVRLLDGGLAAWRGEGFPLETAIPRFEPVVYRGMPDPAMVVTTE
                     DV"
BASE COUNT          545 a          921 c          928 g          618 t
ORIGIN      
        1 atacggaagt atgttagaag tattctagcc ccgtgggagg ctccgtaccg gtcggcacgc
       61 gctgtgggtg tggatacaac cggaactcat gactacgctc tgcaatgagc ataagatgcg
      121 gtgcgtgacc gtcggtaacg aatcgacata ccgtgtcacg ctaatccccg tttttaaatt
      181 gcaggagatg gtcaatgacg atcaaggttg gcgacaggat gccttcgggc gaattcggcg
      241 taatgacgga caagggtccc gggaaaatat ccagcgacga acttttcgcc ggcaagaaag
      301 ttgtccttgt gtcggtgccg ggtgcgttca cgccgacttg ctcgttgaat cacctgcccg
      361 gttttgtaga tcatgccgat gagatcatga ccaagggcgt cgatacgatc gcgtgcatgg
      421 cggtcaacga cgtgttcgtg atgaatgcct ggggtaagga ccggaaggtg ggcgacaagg
      481 tcaggatgct ggcggacggt aacggcgaat acacccgcaa gctcggactc gaaatggacg
      541 gcagcggctt cggcctcggc aagcgcgggc agcgtttcgc catggtcgtc gacgacggcg
      601 tagtgaagca tcttgcggtc gaggagcccg gcaagttcga ggtgagcaaa gccgaggcga
      661 ttctcgcacg gttgtaggag ctgaagctgt ttcgacaggc tcctgcaacc ttgtgctact
      721 tgcgtagagc ttccgtcagt tccggaacga tcgtgaacag gtcgccgaca agccctatgt
      781 cggcaacctc gaaaatcggc gcttccgcat ccttattgat ggcaactatc gttcccgcat
      841 ccttgatgcc ggtgaggtgc tggatggcgc cggatattcc gacagcaatg tagaggtcgg
      901 gcgcgatgat cttgccggtc tggccgactt gcatctcgtt cggcacatag ccggcatcga
      961 cggctgcccg tgacgccccg acggcggcgc cgagtacgtc ggcgagcttg tagatgatgt
     1021 cgaagctctc cgtgccaccc agcgcgcgtc cgccggatac gactgtagac gcggtctgca
     1081 ggtccgggcg gtcggatttg ccggccttca actcgacgaa ccgggtgtgg tccgggattg
     1141 ccgcgtcgac gggcaccttt tcgacggtcg cgccgccctc attccccacg gcctggtatg
     1201 acgctgtccg gacggtggca accaccggct ggccggaagg ggcctcgacg gtaagcacag
     1261 cgttgccagc gtagacaggg cgcttgaagc ggtgactgct ctcgatgttc atgacatcgc
     1321 tgacctggtt gacgccgagt aacgcagcca ctctcggcat caggtcttta ccgaaggtcg
     1381 tcgatggccc gaagatgtgc gagtacccct ccgacagcgc cgcaatctgc ggtgccagga
     1441 cggcggcaag caggtacttg ttggctggct ggtcgacctg gagcaccttg ccgacgttag
     1501 acaggcgcgc ggcagctgcg gcaacctccg atcctgcatc actgaacacc gctacgtgca
     1561 cttccgcgtc cggcagtccg ctggcgcagg caacgcactt cgcggtactt aagttgatcg
     1621 aattcccgtc gtgctctgca atcaccagta tcttcgacat cagacaagtc cttttgcgtt
     1681 cagggccgct accagttcgg caacgtcatt taccatggct cctttcgagc gctgcggcgg
     1741 cgcttcccac gatatttcct tgagcccctg cgccggctgt acgcccaact cctccaggct
     1801 cagctggtcc agcggtttct tcttggcttt catgatgtcc ggcagcttga cgtagcgcgg
     1861 ctcgttcagg cggaggtccg tcgtcaccac gcccggcaac tcgatttcga tcgtttccag
     1921 cccggtgtcg acctctcggg tcactctcgc agtggatccg gagagttcga ccgaggaggc
     1981 gaacgtcgct tgcgggcgct cccacagcgc ggccagcatc tgtccggtct ggttgttgtc
     2041 gttgtcgata gcctgtttgc cgagcagtac gaggtccggc ttctcccgtt cgaccagcgc
     2101 aaggaaaacg cgagcgaccg tcagctggtc gattggcgat cccgtcgtca ccaggatggc
     2161 gcgatcggct cccatggcca gccccgtgcg cagctgttgc tgagcttcgc tgctgccgat
     2221 ggacacggca acaacttcgc tggcttcacc gcgttcacgg atcctcagag cctcttcgag
     2281 agcgatttcg tcgaagggat tgatgctcat tttgacaccg tcggtttcga cgccggtgcc
     2341 gtcggatttc acccgcacgc ggacgttgaa atcgacgacg cgcttcaacc cgaccagtat
     2401 tttctccacc gatggctctc catgacattg cgctgccgcg ccgcaaacgg cgtattgtcc
     2461 ccgacggaat tggcgtgtac aaggcgcatg agaacctggc ccacgacgag cacaacggga
     2521 cgcgcgatgg cttacgagaa tttcctggtg cggccgcacg agctgcatga acacctgcac
     2581 gacgaccact ggcgtgtcgt cgactgtcgc ttcgacctcg cgaacccgga taaaggctac
     2641 tccgagtacc tcgctggtca ccttccgggc gctgtgtacg cgcatctcga tcgcgacctt
     2701 gcggcgccgg taacggggcg caccggccgc caccccctgc cggacccgga agcattcgcg
     2761 ttgacgctcg gcaagctcgg cattcaacga gaatcccacg tcatagcgta cgaccatggc
     2821 agcggcgcca tcgcctcccg gctttggtgg ttgcttcggt ggatgggaca tccatccgtc
     2881 cgcttgctgg atggcggcct cgcagcctgg cggggcgagg gctttcccct cgaaacagca
     2941 atcccgcgct tcgagcctgt cgtttaccgc ggaatgcccg atcccgccat ggtggtaacg
     3001 acggaagatg tg
//
LOCUS       DASUOM010000240         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1008024,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000240 DASUOM010000000
VERSION     DASUOM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008024"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VFY27_05010"
     CDS             complement(<1..1261)
                     /locus_tag="VFY27_05010"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5764906.1"
                     /translation="MRAELAERAQQAVEQALEAGADDVWAVAQQSRDVEFSYRDGALE
                     KVQDATSRNLSVQVYANGRYSSHQTTDLNPDRLGGFLAQAVAGTAALEPDPYRAITPP
                     ELFKDRSTLNLDLVDTAVATLEREQRVAWCVNLDEAARDHERVISATAGVYDGSVAYA
                     IASSNGFEGFRDATYCWLGTSVTLKDRGDRRASDSFYAGGTHVADLPGARGVAETALD
                     RALVRLDADKGPTARTTMLVDSRAASSLVGRLLQSAYASSVQQGRSFWSKLHGKKAFS
                     DRLTLVDEPLIRRGLSSRLFDEEGIAARPLPLVEAGIVRNIYVDTYYGRKANMQPTTG
                     SPSNVTVAAGALSLEELVAEVGEGVYVTSWLGGNADGTTGDFSLGLRGHVIENGAIGR
                     PVGEMNVTGNLRQLFSRLELTGNDLYPY"
     gene            complement(1251..>2519)
                     /locus_tag="VFY27_05015"
     CDS             complement(1251..>2519)
                     /locus_tag="VFY27_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX5764907.1"
                     /translation="RADSEISQGVGLRAVVGDQTGYAFTEDLTLPSMLAAARTAAAIA
                     SGSRVVPPQAYNPKNSGDLYRTQVAWADVGVDSKLPLLQRVDRLARAADPSVDRVSVY
                     WADSDERVLIATLDGHLITDHRPMTRLSAQVTATRNGESQSGFSNIAAREEFSWYTRE
                     RIDTMVREAVDRTLILFDAKRPPAGEMPVILAAGPSGILLHEAIGHGMEADFNRKGTS
                     IYSDMIGKPVAEPFVTVVDQATIPNERGALNYDDEGNECGRTVLVENGILRSYLHDSI
                     SARHFNVAATGSGRRESYRFAPMPRMTCTFMEDGPHTREEIIAAVANGIIAETYTNGQ
                     VQIGAGDYTFYVKNGWLVEKGRVTAPIKDVNIIGNGPESLRRITMVANDGHLDTGGWT
                     CGKNGQQVPVSQGIPTVLVSSMTVGGESAS"
BASE COUNT          433 a          846 c          756 g          484 t
ORIGIN      
        1 catacggata gaggtcgtta ccggtcaact ccagtcggct gaacaactgg cgcaagttgc
       61 cggtcacgtt catctcgccg acgggacgcc cgatagctcc gttctcgatg acatgaccgc
      121 gcaatccgag ggaaaaatcg ccggtggtgc cgtcggcatt gccccctagc caggaggtga
      181 cgtaaacgcc ctcacccacc tcggccacaa gctcctcgag cgaaagcgct ccggcagcca
      241 cggtcacgtt ggacgggctg ccggtcgtgg gctgcatgtt cgctttgcgc ccataatagg
      301 tatcgacgta gatgttcctg acaatgccgg cctcgaccag cggcagcggg cgggcggcga
      361 taccttcctc gtcgaacagc cgcgaactca agccccggcg aatcaggggt tcatcgacaa
      421 gggtcagcct gtcgctgaat gccttcttgc cgtgcagttt cgaccagaat gacctgccct
      481 gctgcacgct gctcgcgtat gccgactgca gaagccggcc gaccagtgac gatgccgctc
      541 gcgagtcgac cagcatcgtc gtgcgcgcgg taggcccttt gtccgcatcg agcctcacga
      601 gcgctcgatc gagcgccgtt tcagcgactc cccgcgcacc cggcaggtcc gcgacatggg
      661 taccgccggc atagaaactg tcggatgcac ggcgatctcc gcggtccttc agcgtcacgc
      721 tggttccgag ccagcaatag gttgcgtcgc ggaaaccctc gaagccattg ctgctcgcaa
      781 tcgcgtacgc cacggagccg tcgtaaacgc ctgccgtcgc ggagatcacc ctttcgtgat
      841 cccgtgccgc ttcatcaagg ttcacgcacc aagcaacccg ttgctcgcgc tcgagagtcg
      901 ccacggcggt gtccaccagg tcgaggttca aggtcgaacg gtctttgaac aattcgggag
      961 gcgtaatcgc ccggtatgga tccggctcga gcgctgcggt ccccgccacc gcctgcgcca
     1021 ggaacccgcc gagccgatcc gggttgaggt cggtggtttg atggctggaa tagcggccat
     1081 tcgcataaac ctggaccgac aggttgcgcg acgtcgcatc ctgcaccttc tccagcgcgc
     1141 cgtcacgata actgaactcg acgtcgcgac tttgctgagc gacggcccag acgtcgtcgg
     1201 caccggcttc gagcgcctgc tcaaccgcct gctgcgcccg ttcggcaagc tcagctcgca
     1261 ctttcgcctc ccaccgtcat gctggatacc agcactgtcg ggattccctg tgagaccggc
     1321 acctgttgac cattcttgcc gcaagtccag ccgccggtgt cgagatgacc gtcattcgcg
     1381 accatcgtta tgcgccgcaa ggattcaggc ccgttgccga tgatgttcac gtcctttatc
     1441 ggcgccgtaa cccgaccctt ttcgacgagc cagccgttct tcacataaaa cgtgtaatcg
     1501 ccggcgccga tctgcacctg cccgttcgta taggtctcgg cgatgatgcc gttggcaacc
     1561 gcggcaatga tttcttcccg cgtgtgcggt ccgtcctcca tgaatgtgca ggtcatccgt
     1621 ggcatcgggg cgaaacggta ggactcgcgc ctccctgaac ccgtggccgc cacgttgaag
     1681 tgccgcgcac tgatggagtc gtgcaggtaa gacctcagga tgccgttttc caccagcacc
     1741 gtgcggccgc attcgttgcc ctcatcgtcg taattgagcg caccgcgctc gttcgggatc
     1801 gtcgcctggt ccaccacggt gacaaaaggt tcggccacgg gttttccgat catgtccgaa
     1861 taaatgctcg tacccttgcg gttgaaatcg gcttccatgc cgtgcccgat cgcctcgtgc
     1921 agcaggatgc cgctggggcc ggccgccagg attacgggca tttcgccggc gggtggccgc
     1981 ttcgcgtcga agaggatcag ggtgcgatcg acggcttccc tgaccatcgt gtctattcgc
     2041 tcgcgcgtgt accaggagaa ctcctcgcgc gcggcgatgt tcgaaaagcc gctctgggac
     2101 tcgccgttgc gggttgccgt aacctgcgcg ctcagtctcg tcatgggcct gtggtcggtg
     2161 atcaggtgcc catccagggt cgcgatcagc acccgctcgt cagagtcggc ccagtacacc
     2221 gaaaccctgt cgaccgacgg atcggcagcc ctcgccagcc gatcgacacg ctgcaacagc
     2281 ggcagcttgc tgtccacgcc aacatcggcc caggcaacct gcgtccggta aagatcgcct
     2341 gagttcttcg ggttgtatgc ctgcggcggg acgaccctcg agccggaagc gatcgctgcc
     2401 gcggtcctgg ccgcggcaag catcgacggc agggtgaggt cttcggtgaa tgcgtaaccc
     2461 gtctggtcgc ccaccacggc ccggaggccg actccctgcg agatctccga gtccgcgcg
//
LOCUS       DASUOM010000241         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_124278,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000241 DASUOM010000000
VERSION     DASUOM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124278"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFY27_05020"
     CDS             complement(<1..855)
                     /locus_tag="VFY27_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5764908.1"
                     /translation="MSLQSATMPFPAQLKTRLQLDPILMAIGTTLLLGGLVILASASL
                     SISDSTIGNPFYYVQRQALAALIGGMAAFICLFVPMHAWQKLSPLMLLLGLALLTVVL
                     IPGVGYTVNGSTRWLRVGFMNVQVSEPARLCLLMFVAGHLVRRNRSLREEFTGFLRPM
                     VVLSLACALLLAEPDFGAAIVLLATALAMLFVAGARIRDFCLFFGVAAIAMAALAITS
                     PYRLQRLTGFLDPWSDPYNSGFQLTQSLIAIGRGQWFGVGLGEGVQKLFYLPEAHTDF
                     VFAVFAEEF"
     gene            complement(852..2234)
                     /gene="murD"
                     /locus_tag="VFY27_05025"
     CDS             complement(852..2234)
                     /gene="murD"
                     /locus_tag="VFY27_05025"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5764909.1"
                     /translation="MNAAPNNKRRPRASVTELKPGQSDLVFGLGATGLSVARYLKRRN
                     RRARFIDSRMNPPGLEELNAIDPRADVILGSVPPGSLKDVGRIIVSPGIADSDPLLKA
                     ARAAKVQVVSDIELFVQEAKAPFIAITGSNGKSTVTTLVALMCEAAGRKVLSGANLGK
                     PALDLLAEEEPEIYVLELSSFQLVRTRRLPAAVAVLLNISPDHLDWHGSASAYREAKY
                     RIFREAKAAVFNRAEPDAEQFIPQGIPRVNFGLDEAAGKNYGVVMEDGQQFLARGAQL
                     LLETSDMALEGSHNHANALAALATGQVLGLHLSPMLQVLTEFPGLPHRMQFVAQIAGV
                     RFINDSKATNVGAAIASVASVDGLVVLIAGGDGKGGDFPSFAEAVHKKLRAAVLIGRD
                     GPAIAAALKGKAPIYQAADMQTAVSVSASIAEEGDTVLLAPACASFDQYENYMERGKD
                     FCRAVEAMAS"
     gene            complement(2231..3313)
                     /gene="mraY"
                     /locus_tag="VFY27_05030"
     CDS             complement(2231..3313)
                     /gene="mraY"
                     /locus_tag="VFY27_05030"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5764910.1"
                     /translation="MLLYLTNYLTQFESGFNVFSYLTLRAILGALTALVLSFVIGPRM
                     IARLSLNQVGQPVRELGPSSHLPKAGTPTMGGALIITAIVVSTVLWADLENPFVWVVL
                     FVTLAFGVIGYVDDYKKLVLQDPKGLSAKQKLSWQSLAAIVAAVALYHLSDESVGHSL
                     LIPYFKDLALPLGMFQVVVSFVFIVGFSNAVNLTDGLDGLAIMPTVLVGGALGVFAYV
                     TGNINFSSYLGIPYVAGTGEILVFCSALAGAGLGFLWFNTYPAQVFMGDIGALSLGAA
                     LGTVAVVTRQEIVLAIMGGVFVVETVSVIIQVASFKLTGRRVFRMAPLHHHFELKGWA
                     EPKVIVRFWIITVILVLIGLASLKIR"
BASE COUNT          726 a         1056 c         1044 g          586 t
ORIGIN      
        1 gaactcctcg gcgaacaccg cgaaaacgaa atcggtatgc gcttcgggaa ggtagaaaag
       61 tttctgcact ccttcaccca ggccgacgcc gaaccactgt ccgcgtccga tggcgatcag
      121 cgactgggtc agctggaacc ccgagttgta cggatcggac cagggatcga gaaagccggt
      181 gagtctctgc aggcgatacg gtgaagttat cgcgagtgcg gccatcgcta ttgcggccac
      241 accgaagaag aggcagaaat cccgtatgcg agcacctgcg acgaacaaca tggcaagcgc
      301 cgtggcgaga agcacgattg cggcgccgaa gtccggttcc gcgagcaaga gcgcgcaggc
      361 aaggctcaat acgaccattg gccgcaggaa gcctgtgaac tcttcccgca aggatcgatt
      421 gcgacgcacc aggtggccgg cgacgaacat cagcaggcag aggcgcgcgg gctccgacac
      481 ctgcacgttc atgaagccga cccgcagcca gcgagtgctg ccgtttacgg tgtagcccac
      541 gccggggata agtaccacgg tcagcaaggc caatcccagc aacagcatca gcgggctcaa
      601 cttctgccag gcgtgcatgg gcacgaacag gcagatgaac gcggccatcc cgccgatgag
      661 cgcggcgagc gcctgccgct ggacgtaata aaaagggttg ccgatcgtgc tgtcggagat
      721 ggagagagac gccgaggcga gaatgacgag gccgcccagc aggagcgtcg tgccgatcgc
      781 catcagtatc gggtctagct gcagccgtgt tttcagctgc gccgggaaag gcatcgttgc
      841 cgactgcagg ctcatgacgc catcgcctcc accgcgcggc agaaatcctt gccgcgctcc
      901 atgtaattct cgtattggtc gaagctggcg caggcgggcg caagcagaac ggtatcgccc
      961 tcttccgcga tggacgcgct caccgacacg gcggtctgca tgtccgctgc ctggtatatc
     1021 ggtgcttttc ccttcaatgc agccgcaata gccggcccgt cgcgaccgat gagtacggcg
     1081 gcgcgcaact tcttgtgaac tgcttcggcg aagcttggaa agtccccgcc tttgccgtcg
     1141 ccgccggcga tcagcaccac gagcccatcc accgatgcga cggatgcaat cgccgcgccg
     1201 acattggtcg ctttagaatc gttgatgaag cgtacgccgg cgatttgtgc cacgaactgc
     1261 atccggtgcg gaagacccgg aaactccgtc agcacctgca gcatcggcga caggtgaagc
     1321 ccgaggacct gtccagtagc gagcgccgcg agcgcgttcg catggttgtg actgccctcg
     1381 agcgccatgt ccgaggtctc cagcaacagc tgcgcgcccc gcgcgagaaa ctgttggccg
     1441 tcttccatca ccaccccgta gttcttgccg gcagcctcgt ccaggccgaa attcacgcgc
     1501 ggtatgcctt gcggtatgaa ttgttcggcg tcgggttccg cgcggttgaa cactgccgct
     1561 ttcgcctcgc ggaagatccg gtacttcgct tcgcggtatg cagacgcgct gccgtgccag
     1621 tcgagatggt ccggggatat attgaggaga actgcaacag cggccggtaa ccttcgcgta
     1681 cgcaccagct ggaaactgga aagctccagc acgtaaattt ccggctcctc ttctgcaaga
     1741 agatcgagcg ccggcttgcc aagatttgcg ccggacaata ccttcctgcc tgccgcctcg
     1801 cacatcagcg caaccagcgt cgtcacggtg cttttgccgt tagaaccggt gatggcaata
     1861 aacggtgctt tcgcttcctg cacgaaaagc tcgatgtcgg agacgacctg caccttggcc
     1921 gcgcgagcgg ccttcagcaa cggatcgctg tcggcgatgc cgggcgaaac gatgatacgg
     1981 ccgacatcct tcagcgaccc gggcggcaca ctgccgagaa tcacatcggc gcggggatcg
     2041 atagcgttga gttcctcgag gcccggcgga ttcatgcggc tgtcgatgaa ccgcgctcgc
     2101 ctgttgcgcc gcttgaggta acgcgccacc gacaggcctg tcgcaccaag gccgaagacc
     2161 aggtccgact ggccgggttt cagttccgtc acggaagcac gcggccgccg cttgttattg
     2221 ggcgctgcgt tcatcgtatc ttcaaactcg cgaggccgat caggacgaga atgacggtga
     2281 tgatccagaa gcggacaatg actttcggtt ctgcccagcc cttcagttcg aaatgatgat
     2341 gcagcggcgc catgcgaaaa acgcgacgtc ccgtcagctt gaaggaagca acctggatga
     2401 tgaccgacac tgtttcgacg acgaatactc cgcccataat cgcgagcacg atctcctgcc
     2461 gggtcacgac ggcgacggtg ccgagcgcgg cgccgaggga gagtgcgccg atgtcgccca
     2521 tgaagacctg cgcgggatag gtgttgaacc agagaaagcc gaggcccgcc cctgcgagcg
     2581 cggagcagaa gacgagtatc tcgcccgtgc ccgctacata cgggataccg aggtaacttg
     2641 agaaattgat gttgccggta acgtaggcga aaacgcccaa cgctccaccg accagcacgg
     2701 tcggcatgat ggccagaccg tcgagcccgt cggtgaggtt taccgcgttg ctgaagccga
     2761 cgatgaacac aaaactcacg acaacctgga acattcccag cggcagggcg aggtccttga
     2821 agtacgggat cagcaaggaa tgtcctaccg actcgtccga caggtggtac agcgccactg
     2881 ccgcgacaat ggcggccagt gactgccacg acagtttctg ttttgccgaa agacctttcg
     2941 ggtcctggag caccagcttc ttgtaatcgt ctacatagcc gatgacgcca aacgccagcg
     3001 tgacgaacag gacgacccag acgaatggat tttcgagatc cgcccaaagg accgtgctga
     3061 cgacgatcgc ggtgatgatc agcgcgccgc ccatggtcgg cgttcccgcc ttcggcaaat
     3121 ggctggaggg gcccagctcc cgcaccggct gccccacctg gttcaggctc agcctggcga
     3181 tcatgcgggg accgatgacg aacgacagca ccagcgccgt aagggcgccg agaatggcgc
     3241 gcaacgtcag gtaactgaac acgttgaacc cggattcgaa ctgggtcagg tagtttgtca
     3301 ggtacagcag cacggtcagg cctcgcgacc ttgaacggaa tcgtcctgca atgcatgcac
     3361 gacacgttcc atgtgcgtcg aacgggaacc cttcaccagc acggtcacgt ca
//
LOCUS       DASUOM010000242         7759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1460583,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000242 DASUOM010000000
VERSION     DASUOM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460583"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFY27_05035"
     CDS             <1..624
                     /locus_tag="VFY27_05035"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5764911.1"
                     /translation="REMREYIEHRLAIAGRKAKDLIKDDAFDTIYRYTGGVPRLINTL
                     CDTTLLCAFAEDITVITSREIKAAVEELNWKEHELSAGTHPEQSASREGRKAEHLMRI
                     EVRNQGETIAEHYFGPGRIIIGRSPDNEIYIQSKFVSRHHAQLSSDQYGCSIEDLNST
                     NGVYIGEERVKKHRLKDGDIVSLGIHELVYSDLRAVQSPAALKERQG"
     gene            complement(650..868)
                     /locus_tag="VFY27_05040"
     CDS             complement(650..868)
                     /locus_tag="VFY27_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764912.1"
                     /translation="MWVSKPIYESLPYFYLLVGAICLAASMYLNHWYWPEVCFGVGLF
                     CLVAGLVVWLKRRDHRDQDRRASQGQDL"
     gene            complement(1145..2590)
                     /gene="tldD"
                     /locus_tag="VFY27_05045"
     CDS             complement(1145..2590)
                     /gene="tldD"
                     /locus_tag="VFY27_05045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX5764913.1"
                     /translation="MTTENSVETVMSRVLAPAGLEEHHLMSALGSINRKDVDAADLYF
                     QISRQESWSVEDGIIREGSFNIDRGVGVRAVCGEKTGFAYSDELMLPALEKAASAARA
                     ISRQGSSKSVHIASRMPSQNLYPAIDPTTSIRDEDKTALIGEIDRQARKLDSRIEQVF
                     VSLVSSQDLILIAAADGTLAADVRPLVRLNVSVILQSNGQREQGYAGGGARAGLEYFL
                     NSELPMEYAREAVRQAVVQLDAKPAPAGAMPVVLGAGWPGILLHEAVGHGLEGDFNRK
                     GVSAFSGRIGQKVASPLCTIVDDGTLPNRRGSLAVDDEGTPGQYNVLVENGVLRGYMQ
                     DRLNARLSGVAPTGNGRRESFAHIPMPRMTNTYMLAGPHDPEEIIQSVERGLYAPNFG
                     GGQVDITSGKFVFSASEAYLIENGKVTTPVKGAMLIGDGPDVLGKISMVGNDLKLDRG
                     VGTCGKDGQSVPVGVGQPTLKIDELTVGGTA"
     gene            complement(2607..3470)
                     /locus_tag="VFY27_05050"
     CDS             complement(2607..3470)
                     /locus_tag="VFY27_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5764914.1"
                     /translation="MPKTTPGEGGRRQRVAAIQMSSVDSVHANFRTAERLLQRAQAEG
                     CILAVLPENFAYIGKRDTDKLAIAESEGSGPIQEFLSRTARRLSLWIVAGSLPLKTPE
                     TELCYGASFVFDAEGRALSCYRKIHLFDVDLPERGESYRESASMDAGDSPRSIQTPAG
                     HLGLSICYDLRFPELYRSLADEGAELFSVPAAFTFATGTAHWHTLLRARAIENLAYVI
                     AAGQHGTHPNGRTTYGHTMIVDPWGRVLAEQETGDGVIVAEIDLPLERKLRREFPVLQ
                     HRRLWSESRDF"
     gene            complement(3477..7331)
                     /locus_tag="VFY27_05055"
     CDS             complement(3477..7331)
                     /locus_tag="VFY27_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX5764915.1"
                     /translation="MKTLLRKLARYLVYLGTALVILLAVAVGLFRLFLPKLPEYQEQI
                     KEWANAAIGMQVEFSGMDARWRLRGPELNFYDAELVSRDNTLTVLNAEEVRVGVGLMR
                     LLLDRELVADRILIRDTTVNVARDTDGTWQVQGIPIDAFTSSRMASTEDAGALTVVGE
                     DIKINFSAPERAQPVALTVDNIEFLRDEMQHGFEASVVLPPSLGSRIDLAASQRLAGA
                     SPRAPWQVFAEGRALNVAGLMQLAPEGSLPAVASGMADVSLWLDVADGDIRSATANFV
                     ADGVVADASSRHAAFGADGRVEFTRDSEGWLVSADDFLLRTAEGAWPRASLQVRVLTA
                     RPGVADRLAGNASYLNLDDLGYVLAWVPPEQRELLQALAPSGVVRDARFSLTGRGSAD
                     EAFELSAELDRVGIAPYEDLPGVRGFSGTIRADQSGGRLEIHSGDLQLDLAGHLVEPL
                     DLVEADGTVIWRQNESGLTLLSDSIRLRNSDIQSASSLQVTIPAGGASPFVDLQSSFS
                     VRDIGSVERYLPVNVVKPGLYRWLSDALVEGRVPEGSTRFAGPLDKFPFDGGEGTFRV
                     DARVENGTLKYSDKWPVVDNMNLELVVENTRLYSFTNSATNAGNSVVNARVDIPDLRQ
                     PVLGIEGRATGTLETIRRFSRESPIASLFGGQLERVTTEGAASFDLVLSYPILDRENY
                     DFTAHIRPEDATVHIEGLPSPISELNGTVMVTREHLSADSLTGRFLDGPVAIELARAD
                     ERQPGHSVIATANGSATAEGLAGHFGSVFADLLDGSAAYDAIVSFPRAGAPDPAPLSI
                     AVRSDLEGMAVNVPPPLGKPLGDERPVSFAIELPGNGRIDTSGNLSDDARWTLSFVRT
                     DAGWDFDRGRLAVGGAAPVQPDIRGLHVEGRTPVLDLDEWLALSRLRGEDGGPGFAER
                     IRSIDVLVDDVYVVGQHLAQHRVIVNRSAMDWAVRLDGEHAIGSLSVPYDFGGARPLT
                     LQMQKLVLPGGDDRLPADETPPDPRDLPQIVVNAEDFSLGERHLGRFAADFQKTSAGL
                     EAARIESSNETFEIRGRAGWVVDESDETGQRSYVAARLTSTDVARTLRSLDYDVGIEA
                     ESLDAQFDVSWSGGPREDFLASLDGTVNVRFGTGQLDEVEPGAGRVFGLMSIVALPRR
                     LSLDFRDVFDRGFGFDEITGTFRLDDGQAYTCDLSLKGPAADVGIVGRTGLVANDYEQ
                     TAVISANVGNTLPVVGAVVAGPQVAAALLIFSQIFKKPLQEMGQIYYGIEGDWSDPAI
                     ESADPQRFAGTSTMAGCLDDGA"
     gene            complement(7364..>7759)
                     /locus_tag="VFY27_05060"
     CDS             complement(7364..>7759)
                     /locus_tag="VFY27_05060"
                     /inference="COORDINATES: protein motif:HMM:NF044325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX5764916.1"
                     /translation="RVMQVLEQSMVRDHARHQITPVSPLGLVEMTRKRTRESLQHILC
                     EDCPNCNGRGFVMTPATVCFEIFREIIRQHRQFKFDEMLVLAHQDIIELLLAEEANGL
                     ADIEAQTGKAIRLQPESLYLRDQFDVVLI"
BASE COUNT         1430 a         2501 c         2361 g         1467 t
ORIGIN      
        1 cgcgagatgc gcgagtacat cgagcatcgt ctggcaatcg ccggacggaa ggcgaaggac
       61 ctgatcaagg acgatgcctt cgatactatc tatcgctata cgggcggcgt accgcgcctg
      121 atcaacaccc tgtgcgacac cacgctgctc tgcgcttttg cagaggacat tacagtgatc
      181 acctcgcgtg aaatcaaggc ggcggtcgag gaactgaact ggaaagagca cgagctcagt
      241 gccggcacgc accccgagca gtcagcctca cgagagggcc ggaaagccga gcacctaatg
      301 cgcatcgaag tacgcaacca gggtgagacc attgccgagc attactttgg tcctggacgc
      361 attatcatcg gccgttctcc cgacaacgag atctacatcc agagcaagtt cgtgagccgt
      421 caccacgcgc agctgtcaag cgaccagtac gggtgctcca ttgaggacct gaacagcacg
      481 aatggcgtct atatcggcga ggagcgggtc aagaagcatc ggctgaagga cggggacatc
      541 gtctcgctcg gtattcacga actcgtttac agcgacctgc gcgccgtcca gtcgcctgcg
      601 gcgctgaagg aacggcaggg gtaaaagcgg agctggcgct tccctggtac taaagatcct
      661 gcccctggct tgcccgccgg tcctgatcgc gatggtcgcg gcgcttcagc cataccacca
      721 gaccggcgac gaggcagaaa agtcccacac cgaagcagac ttcgggccag taccagtgat
      781 taaggtacat cgaagcggca aggcagatgg cgcctaccag caggtagaag tacggcagac
      841 tttcgtagat tggtttggag acccacattt cccgcgcccc ttaagagccc gcgtcatgca
      901 tgaccggctc ttgattgtcc cgcccgcctt tcccgcgagc gggccggctt ccaggctctt
      961 cttcgggaaa ttgtccgtcg cctgcagata ttagtccagt cggcatttgc ggccgccgta
     1021 acgcccgacg tatgcggttg aaatcctgac tggcgcgaaa gtctatggga gccgcgacag
     1081 gattgcggtg aagcgattca caagacggcg ggttcgctga ccggcatctg ttttgttgta
     1141 cggcctaggc agtaccgccg acggtaagtt cgtcgatctt cagggtgggc tggcccacgc
     1201 ccaccggcac cgattgcccg tcttttccgc aggtgcccac gccccggtca agcttcaggt
     1261 cgttaccgac catcgagatc ttaccgagta catccggacc gtcgccgatg agcatcgcgc
     1321 cctttaccgg cgtcgtcact ttgccattct cgatgagata ggcttcgctc gcggaaaaga
     1381 cgaacttccc ggaggtaatg tcgacctgcc cgccgccgaa gttaggggca tacaggccgc
     1441 gttccaccga ctggatgatc tcttccgggt cgtgcggtcc tgccagcatg taggtgttcg
     1501 tcatgcgagg catcggtatg tgggcgaaag actcgcgacg accgttgcct gtcggggcga
     1561 cacccgagag cctggcattc agccggtcct gcatgtagcc acgaagcaca ccattttcga
     1621 ccagcacgtt gtactgcccg ggcgtgcctt catcgtcgac cgcaagcgag ccgcggcgat
     1681 tcggcaatgt gccgtcatcg acgatcgtgc aaagcggcga ggcgactttc tggcctatgc
     1741 gtcccgagaa cgccgagacc cccttgcggt tgaagtcgcc ttccaggccg tgcccgaccg
     1801 cttcgtgcag caggatgccg ggccagccgg cgccgagaac gaccggcatt gcgccggccg
     1861 gcgcgggctt cgcgtccagc tgcacaacag cctggcgtac cgcctcacgc gcgtattcca
     1921 tcggcagctc gctattcagg aaatactcga gacccgcgcg cgcaccgccg ccggcgtaac
     1981 cttgttcgcg ctgtccgtta gattgcagga tgacgctgac attgaggcgg acgagagggc
     2041 gcacgtcggc cgcaagtgtg ccatcggccg cggcaatcag gatgaggtcc tggctgctga
     2101 caaggctcac gaacacctgc tcgatacgcg agtccagctt ccgtgcctgc ctgtcgattt
     2161 cgccgatcag cgcagtcttg tcttcatcgc gtatgctggt ggtgggatcg atagccggat
     2221 acaggttttg cgagggcatc cgtgatgcaa tgtgtaccga cttggaactg ccctggcgcg
     2281 agatggccct ggctgcgctg gctgcctttt ccagcgccgg aagcatcagt tcgtcggaat
     2341 aggcgaagcc ggttttctcg ccgcatacgg cgcgcacgcc gaccccgcga tcgatgttga
     2401 agctgccctc gcggatgatg ccatcctcga ctgaccagct ttcctgccgg ctgatctgga
     2461 aataaaggtc cgctgcgtcg acgtctttgc ggttgattga tcccagcgcc gacatcaggt
     2521 gatgctcttc gaggccggcg ggcgccagca cgcgcgacat caccgtctcg acagaattct
     2581 cagtagtcat taccaattca tattcctcaa aaatctcttg attcactcca caatctgcgg
     2641 tgctgcaaca cgggaaattc cctccgcagt ttgcgctcga gtggcaggtc gatttccgcg
     2701 acgatcacgc cgtcgccggt ctcctgctcc gccagtacgc ggccccaggg atcgacgatc
     2761 atcgtatgcc catacgtcgt gcggccattc gggtgcgtac cgtgttggcc ggcggcgatg
     2821 acgtaggcca ggttctcaat ggcgcgtgcc cgcaggagcg tgtgccaatg cgctgttcca
     2881 gtcgcgaaag taaaagcggc tggtacgctg aatagttccg caccctcgtc tgcgagactg
     2941 cgatacaact ccgggaagcg caagtcatag cagattgaaa gaccgagatg acctgccggt
     3001 gtttgaatcg atcgcggtga atctcccgca tccatgctcg ctgattcacg atagctttca
     3061 ccgcgctccg gaagatccac gtcgaagagg tgaatcttgc ggtagcagga cagcgcgcgg
     3121 ccctcggcgt caaagacgaa cgatgcccca taacatagct ccgtttccgg cgtcttcaag
     3181 ggcaggctgc ctgcgacgat ccatagcgac agccttcgcg ccgtccggga caggaactcc
     3241 tgaatcggtc cgctgccctc gctttccgcg atggccagct tgtcggtgtc gcgcttgccg
     3301 atgtacgcga agttctccgg caggacggcg agaatgcagc cttcagcctg cgcccgctgc
     3361 agcaggcgct ccgcagtgcg gaagttcgca tggacgctgt cgacgctcga catctggatg
     3421 gcggcgacgc gttgcctgcg cccgccttcg cctggagtcg tttttggcat tgctggtcag
     3481 gccccgtcgt ccaggcaacc ggccatggtg ctggttccgg cgaagcgctg cgggtccgct
     3541 gattcgatgg cggggtcgct ccagtcgcct tcgataccgt aatagatctg tcccatttcc
     3601 tgcagcggct tcttgaaaat ctgcgaaaaa atcaggagcg ccgccgctac ctgcggcccc
     3661 gccaccacgg cgccgaccac gggcaaggtg ttgccaacgt tcgcactgat aactgcggtc
     3721 tgctcgtagt catttgccac cagtccggta cgtccgacta tgccgacgtc cgctgcagga
     3781 cccttcagcg acaggtcgca ggtgtaagcc tggccatcgt ccaggcggaa cgtgccggta
     3841 atctcgtcga agccgaagcc cctgtcgaag acgtcgcgaa aatcgagcga caggcgccgc
     3901 ggcaacgcaa cgatgctcat gagaccgaag acccgtcccg ccccaggctc gacctcatcg
     3961 agctgcccgg tgccgaagcg gacgttgacc gttccgtcaa ggcttgcgag aaaatcctcg
     4021 cgcggcccac cggaccagct cacgtcgaac tgcgcgtcaa gcgattcggc ctcaatgccg
     4081 acatcgtagt cgagggaacg gagtgtgcgg gccacatcgg tgctggtgag ccgcgccgcg
     4141 acgtaactcc gctgtcccgt ctcgtctgac tcatccacaa cccagccggc ccggccgcgt
     4201 atctcgaacg tctcgttgct ggattctatc cgggcagctt cgagtcctgc gctggttttc
     4261 tggaaatcgg cagcaaaacg gccgaggtgc cgctctccaa gggagaagtc ctccgcgttc
     4321 acgacgatct gcggcaggtc gcggggatcc ggcggtgtct cgtcagccgg gagacggtcg
     4381 tcgccacccg gcaggaccag tttctgcatc tggagcgtca atggccttgc gccgccgaag
     4441 tcgtacggaa cgctgagcga gccgattgca tgttcgccgt ccagccgcac cgcccagtcc
     4501 atcgcgctgc gattgacgat tacccggtgt tgtgccaggt gctggccgac tacgtagacg
     4561 tcgtcgacca acacatcgat tgaacgaatc cgctctgcga aacccggtcc gccgtcctcg
     4621 ccgcgaaggc gcgagagggc cagccactca tcgagatcca gcaccggagt gcgcccttcc
     4681 acatgcaggc cgcgaatgtc gggctgcact ggcgcggcac ccccgacggc gagcctgccc
     4741 cggtcgaaat cccatcctgc atcggtacga acgaacgaga gcgtccagcg cgcatcgtcc
     4801 gacaggttcc cggatgtatc gatgcggccg ttgcccggca gctcgatcgc aaacgacact
     4861 ggccgctcgt cgcccagcgg cttgccgagc ggcggcggta cgttgacggc catcccctcg
     4921 agatccgacc ggactgcgat cgacagcggc gcaggatcgg gcgcgcccgc gcgagggaaa
     4981 ctcactatgg cgtcataagc agccgagcca tcgagaaggt ccgcaaacac tgaaccgaaa
     5041 tgaccggcaa gaccttcggc ggttgcgctg ccgtttgccg ttgcgatgac gctgtggccc
     5101 ggttgcctct cgtcggcacg tgccagctcg atcgcaaccg gaccgtcaag gaaacggccc
     5161 gtcaacgaat cggcgctgag atgttcgcgc gtgaccataa ccgtaccgtt cagctcgctg
     5221 atcggcgacg gcaatccttc gatgtgaacc gtggcgtcct caggccggat atgcgctgtg
     5281 aagtcgtaat tttcgcggtc gagaatagga tacgacagaa ccaggtcgaa ggaagcggcg
     5341 ccctctgtgg tcacccgctc gagctgcccg ccgaaaaggg aggcaatcgg gctttcccgc
     5401 gagaaccggc gaatcgtctc cagcgtcccc gttgcccggc cttcgatgcc cagcaccggc
     5461 tggcgcaggt ccgggatatc cacccgcgcg ttgacgacgc tgttgccggc attcgttgcg
     5521 ctgttcgtga aggaatacag ccgtgtgttc tccacgacaa gctcgagatt catattgtcc
     5581 acgaccggcc acttgtcgga gtacttcagg gtgccgtttt ccacgcgcgc gtcgaccctg
     5641 aacgtgccct ccccgccgtc gaacgggaac ttgtcgagcg gtccggcaaa ccgggtactg
     5701 ccctccggca ccctgccctc taccagcgca tcgctgagcc accggtacag tcccggtttc
     5761 accacgttga cggggagata gcgctcgacg ctgccgatgt cgcgaacgct gaagctgctt
     5821 tgcaggtcga cgaagggcga cgcccctccc gccgggatcg tcacctgcag gctgcttgcg
     5881 ctctggatgt ccgagttgcg cagccggatg ctgtcggaca ggagcgtcag gccgctctcg
     5941 ttctggcgcc agatgaccgt accgtcggct tcgaccaggt cgaggggctc cacgaggtgt
     6001 cccgcgagat cgagttgcag gtcgccggag tgtatctcca ggcgcccgcc gctctggtcc
     6061 gcccggatag tcccgctgaa tccgcggacg cccggcaggt cctcgtacgg tgcaatgcct
     6121 accctgtcca gctcggccga caactcgaac gcctcgtcgg cggagcctcg gccggtgagg
     6181 ctgaaccggg cgtcgcgcac gacgcccgac ggagcgagag cctgcagcaa ttcccgctgt
     6241 tccggcggga cccaggcgag gacatagccc aggtcgtcga gattgagata ggaagcgttg
     6301 cccgccagcc tgtctgcgac gccaggccgg gcagtcagga cccgcacctg caatgacgcc
     6361 cggggccagg cgccctcggc ggtccgcaaa agaaaatcat ccgcggagac cagccatccc
     6421 tcgctgtccc tggtaaattc cacgcgtccg tcggcgccga acgcggcatg cctgctgctg
     6481 gcgtcagcca cgacaccgtc cgcgacgaaa ttcgcagtag cgctacggat gtctccgtct
     6541 gccacgtcca gccacaggct tacgtcggcc atgccggatg cgactgctgg gaggctgcct
     6601 tcgggcgcga gttgcatgag tccggcgacg ttgagcgccc tcccttcggc gaacacctgc
     6661 cacggagccc tgggtgaagc cccggcaagt cgctggcttg cggcaaggtc aatccggctg
     6721 ccgaggctcg ggggcagcac cacggaagcc tcgaaaccgt gctgcatttc gtcccgcagg
     6781 aattcgatgt tgtcgacggt cagcgcaacg ggctgcgccc gctccggcgc gctgaagttg
     6841 atctttatgt cttcacctac gactgtcagc gcgcccgcat cttccgtcga cgccatgcgg
     6901 gaacttgtga acgcgtcgat ggggatgccc tgtacctgcc aggtgccatc ggtgtcgcgg
     6961 gccacgttca ccgtcgtgtc gcgtatgaga atgcgatccg caaccagttc gcggtcgagc
     7021 agcaggcgca tgaggccaac ccccacacgc acctcctccg cattcagcac tgtgagcgtg
     7081 ttgtcgcgcg agaccagctc tgcgtcgtaa aaattgagtt cagggccgcg caaccgccat
     7141 cgcgcgtcca tccccgagaa ctccacttgc atgccgatcg ctgcgttcgc ccattccttt
     7201 atttgttcct ggtattcggg cagtttcggc aggaacaggc gaaacaggcc gactgcgacg
     7261 gcaagcagga tgaccagcgc cgtgcccagg taaaccaggt atctagccag ctttcgcagc
     7321 agtgtcttca tgtattcaat acggcgtggc gttcgacggc tcttcagatc agcacgacgt
     7381 cgaactggtc cctgagataa agtgattccg gctgcagccg gatggctttt cctgtctgcg
     7441 cttcgatatc ggcgagtcca ttcgcctcct cggcgagcag gagttctatg atgtcctggt
     7501 gcgcgagcac cagcatctcg tcaaacttga actgtcggtg ctggcggatg atttcgcgaa
     7561 agatctcgaa gcatacggtc gccggggtca tgacaaaacc gcgcccgtta cagtttggac
     7621 agtcttcgca caggatatgt tgcaggcttt cgcgggttct cttgcgcgtc atctcgacca
     7681 gtccgagcgg agacacggga gtgatctggt gccgcgcgtg gtcccggacc attgattgtt
     7741 cgagtacctg cattacccg
//
LOCUS       DASUOM010000243         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_244982,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000243 DASUOM010000000
VERSION     DASUOM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244982"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFY27_05065"
     CDS             <1..806
                     /locus_tag="VFY27_05065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5764917.1"
                     /translation="SSYADYVQNRIFRPLGMNDSWIMTKPGDEKGLAAPYEIEDGRAR
                     RMDYEWYSTPPVASAVSSTMDMGRFVIALTEPTIVSRTTLRSMMTTQATLHPEVPGWG
                     YGFQIDSVNSRGVAEHGGDIGGFAGLITIVPDERLGFFVIHHGEGSSLRFVVRQMLLD
                     QLLPQQVAAPAALPAVDLSPYIGSYRASFNCHTCMNPPPVPEFDVTADGGVLELWGNR
                     WVPIGKDLFAREDGRAKLAFVRDHEGRISALTGGSWRVGERITPSEPSQ"
     gene            1104..1352
                     /locus_tag="VFY27_05070"
     CDS             1104..1352
                     /locus_tag="VFY27_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764918.1"
                     /translation="MKSRILVAISIAASALALAGCDVDKTEEGEMPEVDVDVEGGKVP
                     EYDVEGPDVDVGTEEKTITVPDVDVDAPEEGEDPDPDE"
     gene            1498..2016
                     /locus_tag="VFY27_05075"
     CDS             1498..2016
                     /locus_tag="VFY27_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX5764919.1"
                     /translation="MATTRPVTSRDEHAYGRDATARSTALTGGVFLGLGLGGFIDGIV
                     LHQIAQWHNMGSAILPPTTMEAMSQNMRWDGLFHVGTLVLTLIGVLLLWREGRNGTAP
                     SSLRVLTGQIVLGWGIFNLVEGIIDHHLLELHHVRDVPVHVPVYDWWFLGVGGALLIV
                     LGWLMSRTRNAE"
     gene            complement(2090..2530)
                     /locus_tag="VFY27_05080"
     CDS             complement(2090..2530)
                     /locus_tag="VFY27_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764920.1"
                     /translation="MINKTFWIGFIVVYIVWQVIGFVVHGIMLADTYTNMWQVLRPAA
                     EIDSMMWVMFVSSALYLLLFCYIFTKGYEGKGIGEGLRFGLLMGLFMSIPMALDQYAI
                     YPISSNIAVIWLVSGVLSFMIVGAVFAAIYKPSEDTAGRLAAAT"
     gene            complement(2565..3305)
                     /locus_tag="VFY27_05085"
     CDS             complement(2565..3305)
                     /locus_tag="VFY27_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5764921.1"
                     /translation="MSEMQKPDATRIDEIADGLFRIHTPLSVVPGGFSFNQYLLRDDD
                     ALLFHTGPRALFPQVRDAIASVMPLEQLRYVGFSHVESDECGSLNQLLEAAPNAVPLC
                     GRIAAMTSVHDLSDRPPRVLGDGESVQLGRYSACWLDAPHVPHGWENGFLMIDGIDAL
                     LCGDLFMQPGADHPPIVESDILGPSEAMRSRMDYYAHAPDTGSILQRLGGLNPRILAC
                     MHGSAWRGDGAVMLDRLSKALDSRGNEV"
     gene            complement(3423..>5397)
                     /locus_tag="VFY27_05090"
     CDS             complement(3423..>5397)
                     /locus_tag="VFY27_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5764922.1"
                     /translation="EDVPYGASAAWGSTLGADDRFGLVLAASYFNRQYETDLVEGLDW
                     AEFAPGAFAPENVALYDYGIERERIGLNANLEYQPNDSSMWYVRSIFNEFSDWEERDQ
                     LNLDAAGGDQTAVSDTVVQNSEGRATREYRQNDQTQRLINISAGADFTRDNFEWGASY
                     TFSHAEEITPRRVDWEYRSDSDAFPNTIDTSSLFFELDAGTAIHDPANYPFRRVRSRT
                     DDIEEDIHSIKADLRIDMDFGDNPGYLKFGFKYATRDKYRDRTNVNHVAGADFTMADA
                     GLYLPGPDDFHEGRYTLGPVLDFDAHQALLQNSPELFELDAESSALDSIVTDYDIKED
                     IIAGYGMASVDIGNWTVLGGARLEYTDAEYDAWFANLPPADPLNPAEPVNGATDYLDV
                     LPSIHLTYRPTDSIVVRGAWTNTIGRPNFQDVAPALEVEEMEGSAGNENLEPFESMGL
                     DLSFEYYFEPSGIASVGVFYKDIKNPIFTRRIENTVFRGIPLDVLSQPENADSGTLLG
                     LELNLEQQFVGLPQPFDGLGASINLTFVDSEVDVFGREADDLPFFRQPDKIGNLAVFY
                     ALGNFEARIAATYRDDYLQGIGDNVAEDVYFGNRTQVDFKLSFDATDNLTVFGEVQNI
                     NDASRREYQGVAERLFADEIYSWTALFGASYAF"
BASE COUNT         1025 a         1590 c         1701 g         1081 t
ORIGIN      
        1 gctcgagcta cgcggactac gtacagaacc gtatcttccg tcctctcggc atgaacgatt
       61 catggatcat gacgaagccg ggagacgaga agggcttggc ggcgccctat gagatcgagg
      121 atggccgggc gcgacggatg gattatgaat ggtattcgac gccgccggtt gcctcggccg
      181 tgtcgtcgac gatggatatg ggacgctttg tcattgcgct caccgagccc acaattgtgt
      241 cccggaccac gctgcggtcc atgatgacga cccaggcgac tctgcatccg gaggtgccgg
      301 gctggggata cggcttccag atcgactcgg tgaacagtcg cggcgtcgcg gaacatggcg
      361 gggacatcgg cggctttgcg ggcctgatca cgatcgtgcc tgatgaacgc cttggtttct
      421 ttgttatcca ccacggcgaa ggctcctcac tgcgttttgt ggtgcgtcag atgttgctcg
      481 atcaactgct accgcaacag gtcgctgccc ccgcagcatt gcctgcggtg gatctttcgc
      541 cgtatatcgg ctcgtaccgt gcgagcttca actgccacac gtgcatgaat cctccgccgg
      601 ttccggagtt cgacgtcaca gccgatggtg gtgtgctaga gctatggggc aatcgctggg
      661 tgccgatcgg caaggatctg ttcgcccgtg aagacgggcg cgccaagctg gcgtttgttc
      721 gcgaccacga gggacgcatc tccgccctga cgggaggctc atggcgggtc ggcgagcgaa
      781 tcacgccttc agaaccttcc cagtgaggaa gttcgacacg attcagcgag tcctggcgac
      841 ggtcgaacgt cgcttgaaat ggcttaaagg ttcttaggct ccagcgtaag cgccgccgca
      901 cccgctcagt gagccgccgt agcgaccatc attaccggac agtcgataaa gcgccacggc
      961 gtgcccagcc tcatgtaaag ccgtggtgcg gagatcgtca ttcttctggc acaactcttg
     1021 cacatgtacc ttaaacgagg tgctacgcgc acaggagtgg tgcgggcaca gggattagtt
     1081 cgaggtggag aaggaagcaa cttatgaaga gcagaatctt agtagctata agcattgcgg
     1141 cgtctgcgtt ggcactggca ggctgcgacg ttgataagac cgaggaaggt gagatgcccg
     1201 aagtcgacgt cgatgtcgag ggaggcaagg tgccggaata tgatgtcgag ggaccggatg
     1261 tagatgtcgg aacggaagaa aaaaccatca cggtgcctga cgtggacgtc gatgctccgg
     1321 aagaaggtga agacccggac ccggacgaat aaaagccggc attagctctg tcaagccgga
     1381 gagcctcgca accaggaggt tctccggctg atgtaagcca cgacagccgt cccttggatc
     1441 ctgctcgggt gcgttggcat gggttatgcc gctgagttct ccgacgggag gtacatcatg
     1501 gcaaccacca gaccagtcac ttcacgcgac gagcacgctt acgggcggga cgcgaccgcg
     1561 cgatccactg ccctgaccgg cggtgtgttt ctcggccttg gcctcggcgg cttcatcgac
     1621 ggaatcgttc tgcatcagat tgcacagtgg cacaacatgg gcagcgccat cctgccgccg
     1681 acaacgatgg aagcgatgtc gcagaacatg cgttgggacg ggttgttcca cgtcgggacg
     1741 ctcgtgctga cactgatcgg tgtgctgctt ctgtggcgtg agggcaggaa cgggactgct
     1801 ccatcctcgc tcagggtgct gaccggccag atcgtgctcg gatggggcat cttcaacctg
     1861 gtcgaaggga tcatcgacca tcacctgttg gaacttcacc acgttcgcga cgtgcccgtt
     1921 cacgtaccgg tgtacgactg gtggttcctg ggtgttggcg gcgctctgct tatcgtcctc
     1981 ggctggttga tgtcgcgcac cagaaacgca gagtaaccat gccggcggtc aggaccgtcg
     2041 gttgaagagg agagccccgc cgcgacgagg ctccggttcg ttcaggaggt caggtcgccg
     2101 ccgccagcct gcctgccgtg tcctcgctcg gcttgtatat cgccgcgaac acagctccta
     2161 cgatcatgaa gctgagcacc ccgctcacaa gccatatcac cgcgatgttc gacgagatgg
     2221 gatagatcgc gtactggtcc agcgccatcg ggatcgacat gaacagcccc atcagcaggc
     2281 cgaagcgcag gccttcgcct atgcctttgc cttcgtatcc cttcgtaaag atgtaacaga
     2341 acagcagcag gtagagtgcg ctgctgacga acatcaccca catcatgctg tcgatctcgg
     2401 ctgctggccg caacacctgc cacatgtttg tgtaggtatc cgcaagcatg attccgtgca
     2461 cgacaaagcc tattacctgc cagacgatgt agacgacgat gaaccctatc cagaaagtct
     2521 tgttgatcat ttcgctttcc tttttgccag gtctcgtcgt tacgctagac ctcatttccg
     2581 cgggaatcaa gggctttgga gagcctgtcc agcataacgg ctccgtcgcc gcgccaggca
     2641 gatccgtgca tgcaagcgag gatcctgggg ttcaggccgc cgagcctttg aagaatggac
     2701 cctgtatcag gcgcatgtgc gtagtaatcc atcctggacc gcatcgcctc gctgggaccg
     2761 agaatgtcac tctcgacgat cggcgggtgg tcggcacccg gctgcatgaa caggtctccg
     2821 cacagcagcg cgtcaatccc gtcgatcatc aggaaaccgt tctcccagcc gtgcgggacg
     2881 tgtggcgcgt ccagccagca agccgagtaa cggccgagtt gcacgctttc accgtctccc
     2941 agtaccctgg gtgggcgatc cgaaagatcg tgcacggacg tcatcgcggc tatgcgaccg
     3001 cacagcggca ccgcgttcgg cgcggcctcc aggagctggt tcagcgaacc gcactcgtcg
     3061 ctctccacgt gggagaaccc gacgtagcgg agctgttcga gcggcattac gctcgcgatc
     3121 gcgtcgcgga cctgcggaaa cagagcgcgc ggtcccgtgt ggaacagcag cgcatcgtcg
     3181 tcgcgcagca ggtactggtt gaatgaaaag cctcccggca caaccgacaa tggtgtgtgg
     3241 atgcggaaga ggccatcggc aatctcatca atgcgggtgg catcgggctt ttgcatttcg
     3301 ctcatggtgc tggtcctcgg caaggagcag gtgagcgatt ctagcaccgg gccgttcctt
     3361 ggcgatctgg tgccaggagt gagggtgccg gcgctctcgt ccctgcctgc gccggcacta
     3421 tgtcagaacg cgtaactcgc cccgaacaac gccgtccaag agtagatctc gtcggcaaac
     3481 aggcgctcgg caacgccctg gtattcccgc cgcgaggcat cgttgatgtt ctgcacctcg
     3541 ccgaacacgg tcaggttgtc cgtcgcgtcg aaggaaagct tgaagtccac ctgggtgcgg
     3601 ttgccgaagt agacgtcctc cgcgacattg tctccaatgc cctgcaggta atcatcgcgg
     3661 taggttgcgg cgatgcgggc ctcgaagttg ccgagtgcgt agaagacggc aaggttgccg
     3721 atcttgtcgg gctgccggaa gaacggcagg tcgtcggctt cgcggccgaa cacatccacc
     3781 tcggaatcca caaacgtgag gttgatggag gcgccgagcc catcgaacgg ttggggcagc
     3841 ccgacgaact gctgctcgag gttcagttcc agtcccagca gcgttccgct gtcggcattt
     3901 tccggctgtg acaacacatc gagcgggatg ccgcggaaca ccgtgttctc aatgcggcgc
     3961 gtgaagatgg ggttcttgat gtccttgtag aaaacgccta ccgacgcgat cccggacggc
     4021 tcgaagtagt attcgaacga caggtcgagt cccatcgact cgaacggctc aaggttctcg
     4081 ttgcccgccg agccttccat ctcttcgact tccagcgcgg gagcgacatc ctggaagttc
     4141 ggccggccga tggtgttcgt ccaggcaccg cgcacgacga tactgtcggt cggcctgtac
     4201 gtcaggtgga tgctcggcag tacgtcgagg taatcggtgg cgccgttgac gggctcggca
     4261 ggattgaggg gatcggccgg aggcaggttc gcgaaccagg cgtcgtactc ggcgtcggtg
     4321 tattcgaggc gcgcaccgcc cagcacggtc cagttgccga tatccacgct ggccatgccg
     4381 tagccggcga tgatgtcttc cttgatgtcg taatcggtca cgatcgagtc gagcgccgag
     4441 ctttcagcgt ccagctcgaa cagttcaggg ctgttctgca acagcgcctg gtgggcatcg
     4501 aagtccaaca ccggaccgag cgtatacctt ccttcgtgga aatcgtcggg acctggcagg
     4561 tacaagccgg catcggccat cgtgaagtcg gcgccggcca cgtggttcac gttcgtccgg
     4621 tcgcggtact tgtcgcgcgt cgcgtacttg aaaccgaatt tcaggtaccc ggggttgtcg
     4681 ccgaaatcca tgtcgatccg caggtcggcc ttgatcgagt ggatatcctc ctcgatatcg
     4741 tccgtacgcg accttacgcg gcggaacggg taattcgccg ggtcatgtat cgccgtgccc
     4801 gcatcgagct cgaaaaacag gctggacgtg tcgatcgtgt tcgggaaagc gtcggagtcc
     4861 gagcgatact cccagtcgac ccggcggggc gtgatctctt cggcgtgtga gaacgtatag
     4921 cttgcgcccc attcgaagtt gtcgcgggtg aaatccgcgc cggccgagat gttgatcagc
     4981 cgctgtgtct ggtcgttctg ccggtattcc cgcgtggccc gcccctcgga attctgcacc
     5041 accgtatcgg aaaccgcagt ctggtcccca cctgcggcat cgaggttgag ctggtcgcgt
     5101 tcttcccagt ccgagaactc gttgaagatc gagcggacgt accacatgga actgtcgttc
     5161 ggctggtatt cgaggttcgc gttcaggccg atgcgctcgc gttcaatgcc gtagtcgtag
     5221 agtgcgacgt tctcgggagc gaaagcaccc ggcgcaaact ctgcccagtc gaggccttcc
     5281 accaggtccg tttcgtactg gcggttgaag tagctggccg cgaggacgag gccgaagcgg
     5341 tcgtcggcgc cgagggtaga gccccacgcg gcgctggcgc cgtaaggcac gtcctcg
//
LOCUS       DASUOM010000244         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1342618,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000244 DASUOM010000000
VERSION     DASUOM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342618"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFY27_05095"
     CDS             complement(<1..323)
                     /locus_tag="VFY27_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5764923.1"
                     /translation="MRALSLAMRTLMREWRSGELGVLLLAITVAVAALTGVGFLVDRI
                     AIAVDNQAAEVLAADVVLESGLAMDDKAIEEARRRDLQVARKTGLFSVVFNGDASQLT
                     SLRAVS"
     gene            complement(331..993)
                     /locus_tag="VFY27_05100"
     CDS             complement(331..993)
                     /locus_tag="VFY27_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5764924.1"
                     /translation="MSVLKAESLTKKVTSPEGMLTIVDDVSLDIAAGESVAIVGASGA
                     GKSTLLALLAGLDSPTSGRVTLAGADLTSLDEDGRARLRAERVGFVFQSFHLVPSLTA
                     LENVMLPLELAGRRDAREASLETLGRVGLEHRTGHYPRQLSGGEQQRVALARAFVTRP
                     AVLFADEPTGNLDTQTGERIVALLFDLNAQSHTTLILVTHDRAVAGRCGRLLQMDSGK
                     LK"
     gene            980..1627
                     /locus_tag="VFY27_05105"
     CDS             980..1627
                     /locus_tag="VFY27_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX5764925.1"
                     /translation="MSTLIRLFRVLWVLALLGPVIAQGAAPRTIVVLGDSLSAGYGIS
                     VREGWVNLLAQRLASEGYGYKVVNASVSGETTQGGVARLRRVLELHKPAVVVIELGGN
                     DGLRGLPLAASRDNLQRAITLARNAKARVLLVGMMIPPNYGQRYSQEFRAMFTGLAAK
                     NSVALVPFLLDEVALNADLMQDDGIHPNAKGQPRMLENLWPKLQPLLVAARKPSG"
     gene            1653..3320
                     /locus_tag="VFY27_05110"
     CDS             1653..3320
                     /locus_tag="VFY27_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5764926.1"
                     /translation="MEKHWLKSYPPGVRPEIDVNEYPSLKEMIEKGLAVHATRDAYVN
                     MGKALTFGDLDILSAQFGAFLQTTCGLKKGDRVAIMMPNCLQYPIAVHGALRAGMTVV
                     NTNPLYTTRELEHQLTDSGAIAIVILENFAHVLQEVISHTPIKHVVVTSVGEMLGFPK
                     GAIVNWVVRKKRKQVKPYHLPGAKNFKQALQDGSGHRLQPVKLTHDDLAFLQYTGGTT
                     GVAKGAMLTHGNMVANVLQAMEWISPAFPRDPATLITALPLYHIFALTANCLLFVVLG
                     WRNILITNPRDFPAFVKELKKYRFTFISGVNTLFNALLHTPGFEKVDFTALRCTLGGG
                     MAVQEAVAKRWKEVTGKVLTQAWGLTETSPAACINLPEADFNGSIGLPIPSTEISIRD
                     DDGNELGINVVGEICVRGPQVMAGYWNRPDETAKVMIGKDWLRTGDIGRIDETGLVFI
                     EDRKKDMILVSGFNVYPNEVESVAVTHPGVLEAAAIAQADEKSGEVVALFVVKKDPNI
                     TEQDVIEHCRRALTGYKVPKHVYFRTDLPKTNVGKILRRALRDELPK"
BASE COUNT          685 a         1130 c         1043 g          615 t
ORIGIN      
        1 gaaacggccc gcagcgacgt caactgactc gcatcgccgt tgaagaccac gctgaagagc
       61 cctgtcttgc gagccacctg cagatcgcgg cgtcgcgctt cttcgatggc tttgtcgtcc
      121 atcgcgagac cggactcgag gacgacgtcg gcagccagga cttcagccgc ctggttgtcg
      181 acggcgattg caatgcgatc gacgaggaag ccaacgccag tcagcgcggc gaccgccacc
      241 gtgatcgcaa gcagcaacac gcccagttcg cccgagcgcc attcgcgcat cagcgttcgc
      301 atggccagcg acagtgctct catggcgtcg tcacttcagc tttcccgaat ccatctgcag
      361 gagacgcccg cagcggccgg cgacggcgcg atcgtgcgtg accaggatga gggtggtatg
      421 cgactgcgca ttgagatcga acagcaacgc cacgatgcgt tcgccggttt gagtatccag
      481 gttgcccgtg ggctcgtccg cgaagagcac cgccggccgg gtcacgaagg cacgggccag
      541 ggccacgcgc tgctgctccc cgcccgacag ctgccgggga tagtgccccg tgcggtgctc
      601 cagtccgaca cgacccaagg tctccaggga agcctcccgg gcatcgcgtc ggccggcaag
      661 ctccagcggc agcatcacgt tttcgagcgc cgtgagcgac ggcaccaggt ggaaggattg
      721 aaagacgaat ccgacccgct ccgcgcgcag gcgggcgcgt ccgtcctcat cgagcgaggt
      781 caagtcggcg cccgcgagcg tcacccggcc ggatgtcgga gaatccaggc cagccagcag
      841 cgccagcagg gtcgacttgc ccgccccgga tgcgccgaca atggcaactg attcacccgc
      901 tgcgatgtcc aacgacacgt cgtccacaat ggtcagcatc ccttccgggc tggtaacctt
      961 tttcgtgagg ctttctgctt tgagtacgct cattcggctt tttcgtgtgt tgtgggtgct
     1021 ggcactgttg ggcccggtca ttgcccaagg cgccgcgccc cgcaccatcg tcgtgctcgg
     1081 cgacagtctg agtgccggct acggcatcag tgttcgggaa ggctgggtca acctgctcgc
     1141 gcagcgactg gcctccgaag ggtacgggta caaggtggtc aacgccagcg tcagcggcga
     1201 aaccacgcaa ggaggagtcg cccggctgcg gcgcgtgctg gagctgcaca agcccgccgt
     1261 cgtggtcatc gagctcgggg gcaacgatgg actgcgcgga ttaccccttg ccgccagccg
     1321 tgacaacctg caacgagcga tcaccctcgc ccggaacgcc aaagcccgcg tactgctggt
     1381 ggggatgatg atcccgccta actacgggca gcggtacagc caggaatttc gcgccatgtt
     1441 caccggcctg gccgcgaaga attccgtcgc actcgtcccg ttcctgctcg acgaggtagc
     1501 cctgaatgcc gatctcatgc aggacgacgg cattcacccc aacgccaagg gacagcctcg
     1561 catgctcgag aacctctggc cgaagctcca accgttgcta gttgccgcgc gcaaaccctc
     1621 aggctagcct gcgtacttca acgggggtgg tcgtggaaaa acactggctc aagtcctatc
     1681 cgcccggggt tcgccccgag atcgatgtca acgagtatcc ctctctcaag gagatgatcg
     1741 agaaggggct cgccgtccac gccacccgcg acgcgtacgt gaacatgggc aaggccctca
     1801 cgtttggtga tctggacatc ctctccgcgc aattcggtgc attcctgcag acgacctgcg
     1861 gcctcaagaa gggcgatcgg gtcgcgatca tgatgcccaa ctgcctgcaa tatcccattg
     1921 ccgtgcatgg cgcgttacgc gccgggatga cggtagtcaa caccaacccg ctgtacacca
     1981 cgcgcgagct cgagcatcag ctcaccgatt ccggtgccat cgcgatcgtg atcctggaga
     2041 atttcgcgca cgtcctccag gaagtgatct cgcacacccc catcaagcat gtcgtcgtca
     2101 ccagcgtcgg cgagatgctc ggcttcccca agggtgccat cgtgaattgg gtggtgcgca
     2161 agaagcgcaa gcaggtcaaa ccgtaccacc tgcccggagc gaagaacttc aagcaggcgc
     2221 tccaggatgg cagcggccat cgcctgcagc ccgtcaagct gacgcacgat gatctcgcgt
     2281 tccttcaata cacgggcggc accaccgggg tggccaaggg cgccatgctc acgcacggca
     2341 atatggtcgc caatgtgctt caggccatgg aatggatcag cccggcattc ccgcgggacc
     2401 ctgccacgct catcaccgcg ctgccgctgt atcacatctt cgcactcacg gcgaattgcc
     2461 tgctgttcgt cgtgctcggc tggcgcaaca tcctcatcac caatccgcgg gatttcccgg
     2521 ccttcgtcaa ggaactcaag aagtacaggt tcaccttcat cagcggcgtc aacacgttgt
     2581 tcaacgcgtt gctgcacaca ccaggcttcg aaaaagtgga cttcaccgcc ttgcgatgca
     2641 cgctcggcgg cggcatggcc gttcaggaag ccgttgccaa gcgctggaag gaagtgactg
     2701 gaaaggtgct cactcaggcc tggggcctca ccgaaacgtc acccgccgcc tgcatcaatc
     2761 ttcccgaggc cgacttcaac ggttccattg gtcttccgat tccgtccacc gagatcagca
     2821 tccgggacga cgacggcaat gagctcggga tcaatgtcgt gggcgagatc tgcgtacgtg
     2881 gcccgcaggt gatggctggc tactggaacc gccccgacga gaccgcgaag gtcatgatcg
     2941 gcaaggactg gctgcgcacc ggcgacatcg ggcgaatcga cgagaccgga ctcgtcttca
     3001 tcgaagaccg caagaaagac atgatcctgg tgtcgggctt caacgtgtat ccgaacgaag
     3061 tcgagagcgt ggccgtcact catcccggcg tgctcgaggc cgccgcaatc gcccaagcgg
     3121 acgagaaatc cggcgaagtc gtcgccctgt tcgtcgtgaa gaaggatccg aacatcaccg
     3181 agcaggatgt catcgagcac tgccgtcgtg cgctgactgg ctacaaggtg ccgaagcacg
     3241 tgtattttcg gaccgatctg cccaagacga acgttgggaa gattttgcga agggcgctga
     3301 gggatgagtt gccgaagtag ttataccctt gtagctagcg cggctgcctt tctctggggt
     3361 tgaacaaagg catacgccac cacacccgcc tccgtactca atgctgcgtt agcaggagcc
     3421 ctccttacat tgcgagcctc cagccagtca tcaactgtct tgatgaagct aac
//
LOCUS       DASUOM010000245         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_759942,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000245 DASUOM010000000
VERSION     DASUOM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759942"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="rseP"
                     /locus_tag="VFY27_05115"
     CDS             <1..774
                     /gene="rseP"
                     /locus_tag="VFY27_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5764927.1"
                     /translation="VIRVGSDRARLTEPGVLFEGLGFKPWQPPAVVGMVEEESPAGDA
                     GIVEGDRIVEIAGNRSASFGDLQEVVAARPGETVSVRLMRDGQERVLDVELGAREVDG
                     RQQGFMGIGIDAAAGDHWYVRKFGPFAAIGESVERTWNSTGFTVKMLARMVTGEVSIK
                     NISGPINIAQYAGDSAAAGLNAFLTFLALVSISLGVLNLLPVPILDGGQIVYQTVELV
                     KGSPLSDRSQLVGQQVGILALLLLMSFAFYNDIARILNQ"
     gene            771..3206
                     /gene="bamA"
                     /locus_tag="VFY27_05120"
     CDS             771..3206
                     /gene="bamA"
                     /locus_tag="VFY27_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5764928.1"
                     /translation="MTCLSKPTVSGRFNTLWLWAALLVLAASVSSPVQAQSVAGDEFV
                     VRDMRVEGLQRISEGTVFNYLPINVGDTVDSIRIQEAIRALYAQGMFDDIEMRRDGNA
                     LIVAVKERPSIEDFTIEGNKDIKTEDLMESLRNVGLARGRTFDRSVLDNVQQFLREQY
                     YDRGKYAVVVDANVEERPNNTVRIAINVKEGERARIRQVNIVGNHSFDEEEIREGFTL
                     DTANWLSWIRQDDRYAKEALSGDLEILRSFYMDRGYADFRVESTQVAISPDKKDIYVT
                     INIHEGEQYSISEVKLVGKMVVPEQQLRGLILAQPGSTFNLRLLTQSSELMSFRLGEE
                     GYANAEIEPVPELDHEKKEASVSFFVDAGSRVYVRRINFTGIDEVDDEVLRREIRQAE
                     GAYLSNRLVDRSKILLQRLPFVEKVEVENVPVDGSPDLVDVNFDIEYRMPGQFSGGVG
                     YSESQKIILNGSIVHTNFLGSGNRVALNLNSGRYSKLYSLSHTDPYVTMNGVRRTVSI
                     NHRDITQFTSGSSDFSTTSSGASLEYGYPITEYQSLSVGISYQHAELLSSNSSTQQAQ
                     DWVLNNGNPFIEQVGGGLTFFGTEFDTFELLAGWSYDSRNRAIFADRGTRQQVYLSMA
                     APGSDVEYWTARYNFTKYIPIYGQWTLRLNSELAYGEALKDTTALPPYKQFYGGGPES
                     IRGYKESYMGPRDSFGNPYGGNVLVANQVELIIPIPEKFSSQARASLIYDIGNIFNTG
                     EVQFTDKLGSPVEYKPDFDELRTSVGVAVQWLAPLGFFRFSYAYPLNNYEGNDRFYGD
                     EIERFQFSIGQAF"
     gene            3222..>3562
                     /locus_tag="VFY27_05125"
     CDS             3222..>3562
                     /locus_tag="VFY27_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX5764929.1"
                     /translation="MIFVQKRFLRAFAAVALMSVVSLPLAAQEVKIGVVNVTALMEQA
                     PQAKAAMDALQEEFAPRQRTILAKQQELEDLSEKANRDAAVMGETERRNMEKDLRDLQ
                     REVARMQTEFR"
BASE COUNT          762 a         1028 c         1080 g          692 t
ORIGIN      
        1 gtcatcaggg tcggcagcga tcgggcgcgc ctgaccgaac ccggtgtgct gttcgaaggg
       61 ctggggttca agccctggca gcccccggcc gtggtcggaa tggtcgagga agagtcgccc
      121 gcaggcgatg caggtatcgt cgagggtgac cgcatcgtgg agatcgccgg caatcgcagc
      181 gccagtttcg gtgacctgca agaggtagtc gctgcgcggc cgggcgagac cgtgagcgtg
      241 cggctcatga gggatggcca ggagcgcgtg ctcgacgttg aactgggtgc gcgtgaggtg
      301 gacggccggc agcaaggctt catgggcatc ggtatcgacg cggccgccgg agatcactgg
      361 tacgtccgca agttcggacc ctttgccgcc atcggcgaat ctgtcgaacg cacctggaac
      421 tcaaccggct tcaccgtcaa gatgctggcg cggatggtga cgggcgaggt ttcgatcaag
      481 aacatcagcg gcccgatcaa cattgcccag tacgccggtg actccgcagc cgccggcctg
      541 aacgctttcc tgactttttt ggcgctggtc agcatcagcc tcggcgtgct gaacctgctg
      601 ccggtcccga tactcgacgg cggccagatc gtctaccaga ccgtggaact cgtaaagggc
      661 agcccgctgt ccgatcgctc gcaactcgtc gggcagcagg tggggatcct cgccttgctg
      721 ctcctgatga gtttcgcttt ctataacgac atcgcaagaa tcctgaatca gtgacctgcc
      781 tctcgaagcc gacggtatcg ggccgtttca atactctttg gttatgggcc gccctgctgg
      841 tgcttgccgc atctgtaagc tcaccggtgc aggcgcagtc agttgccggc gatgaattcg
      901 tcgtgcgcga catgcgcgtc gaagggctgc agcgcatttc ggaaggcacc gtattcaact
      961 acctgccgat caacgtcggc gacaccgttg actctatccg aatccaggaa gcaatccgcg
     1021 cgctctacgc gcagggtatg ttcgacgaca tcgagatgcg ccgtgatggc aatgcgttga
     1081 tcgtcgctgt gaaggagcga ccgtcgatcg aagacttcac gatcgagggt aacaaggata
     1141 tcaagaccga ggacctcatg gagtccctgc gcaacgtcgg gctggctcgc ggacgcacct
     1201 tcgaccgttc cgtgctggac aacgtgcagc agttccttcg ggagcagtat tacgaccgcg
     1261 gcaagtatgc cgtcgtcgtg gatgcgaacg tcgaagaacg cccgaataat accgttcgta
     1321 ttgcgatcaa cgtgaaggaa ggcgagcgcg ccaggatccg ccaggtgaac atcgtcggca
     1381 atcacagctt cgacgaagag gagatccgcg aggggttcac gctcgatacg gcgaactggc
     1441 tgtcatggat tcgccaggat gaccgctacg cgaaggaggc gctgagcggc gacctcgaaa
     1501 tcctgcggtc cttttacatg gaccggggct acgctgactt tcgcgtcgag tcgacgcagg
     1561 tagcgatttc gccggacaag aaagacattt acgtcaccat caacatccat gagggcgagc
     1621 agtactcgat ttccgaggtc aagctggtcg gcaagatggt cgttcccgag cagcagctcc
     1681 ggggcctgat cctcgctcag ccgggcagta cgtttaacct gcgcctgctg acccagtcga
     1741 gcgaactcat gtcgttccgc ctcggcgaag aagggtatgc gaacgctgaa atagaaccgg
     1801 taccggagct ggatcacgaa aagaaggaag cttcggtttc atttttcgtc gacgccggga
     1861 gccgggtgta cgttcgccgc atcaatttca ccgggatcga cgaagtcgac gacgaggtac
     1921 tgaggcgtga aattcgccag gcggaaggcg cgtacctttc gaatcgcctg gtcgaccggt
     1981 ccaagattct cctgcagcgg ctgcccttcg tcgaaaaggt cgaagtcgag aacgttcccg
     2041 tggacggcag ccccgacctg gtggatgtca atttcgacat cgaatatcgc atgcctggac
     2101 aattcagcgg cggcgtcggg tactccgaat cgcagaaaat cattctgaac ggcagcattg
     2161 tccacaccaa cttcctgggc tccggtaacc gggtggcgtt gaacctgaat tccggccgct
     2221 actcgaaact ctacagcctg tcgcacactg atccttacgt caccatgaac ggcgtgaggc
     2281 gcaccgtctc gatcaaccat cgtgacataa cgcagttcac gtcgggatcg tcggacttct
     2341 cgacgaccag ttcgggagcg tcgctcgaat acggttaccc cattaccgag taccagagcc
     2401 tgagcgtcgg catctcctac cagcatgcgg agttgctgtc gtcgaactcg agtacccagc
     2461 aggcccagga ctgggtcctg aacaacggca acccgtttat cgaacaggtc ggcggcggcc
     2521 ttacgttttt cggcaccgag ttcgacacct tcgaattact ggctggctgg tcgtacgaca
     2581 gccgcaaccg cgccatcttt gcggatcggg gaacacgcca gcaggtgtat ctcagcatgg
     2641 cggcaccggg cagcgacgtc gagtactgga cggctcgcta caacttcacc aagtacattc
     2701 cgatatatgg tcaatggacg ctacggctga attccgagct ggcatatggc gaagcgctca
     2761 aggacaccac ggcactgccg ccttacaagc agttttacgg aggcgggccg gaatccatac
     2821 gcggatacaa ggagtcgtac atgggcccga gagacagttt cggtaaccct tacggcggca
     2881 acgtgttggt agcgaatcaa gtagagctta taatccccat accggaaaaa ttcagcagcc
     2941 aggcgcgcgc aagccttatc tacgatatcg gcaatatctt caacacgggc gaggttcaat
     3001 ttaccgacaa gctgggtagc ccggtcgagt ataaacctga cttcgacgag cttcggacct
     3061 ccgtaggtgt ggcggttcaa tggctcgcgc cgctcggctt tttccgcttc agctatgctt
     3121 accccctgaa caattacgag ggtaacgacc gcttttacgg agatgagatc gaaagattcc
     3181 agttctccat tggacaagcc ttttaaccat cggacgcaaa tatgatcttt gtacagaaac
     3241 gatttcttcg ggcattcgca gcagtggctc tcatgtccgt ggtgagcctg ccgctcgcag
     3301 cgcaggaagt gaagattggt gtggtcaacg tgaccgccct gatggaacaa gcgccgcagg
     3361 caaaggccgc catggacgcg ctgcaggaag aatttgcgcc ccggcaacgg accatcctcg
     3421 cgaagcagca ggaactcgag gacctttccg agaaggcgaa tcgcgatgcg gcggtcatgg
     3481 gcgagaccga gcgccgcaac atggagaagg accttcgcga cctgcagcgg gaagtagcac
     3541 gcatgcagac ggagttccgc ga
//
LOCUS       DASUOM010000246         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1610359,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000246 DASUOM010000000
VERSION     DASUOM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610359"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1559)
                     /locus_tag="VFY27_05130"
     CDS             complement(201..1559)
                     /locus_tag="VFY27_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX5764930.1"
                     /translation="MSGSRELVPVDRVTALQVYDLGHHIALKEAVRGPAKLGAYIIGI
                     FVIGFGGFAGTVPLAGGAVAPGIISPDGSRRTVQHLEGGIIRTLSVRDGDIVEKGQPL
                     LVLENVQAKANYDLLLKQQRTLRVTKARLDAERTGKDEVEFPPDLATAGPDVTTMMAA
                     QQELFLVRQTSHESKNRILRQRMGQLREQIKGFEAQVTSASRQIDFLDEEIVGKRELA
                     KRGYLAKPELLRMLRMQAELTGRLGQYEAAISEAQQQIGEAELQLLAYDATRADQIAT
                     QLDQVRAELNGLDEKLLASGDVLTRTVIPAPLAGTVVNLKFKTVSGVIQPGVPILDIV
                     PLDEKLIIEARISPTDVDVVQQGLQAQIQLTAFSSRAAPRIAGTVRSVSADRMVDESS
                     KAYYLARVEVDRDELHRVGASVELVPGMPVDVLIVTGERTMANYLLRPLLDAIWKTLR
                     EA"
     gene            complement(1605..3338)
                     /locus_tag="VFY27_05135"
     CDS             complement(1605..3338)
                     /locus_tag="VFY27_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX5764931.1"
                     /translation="MSRAFPSRNSELQQAIASCRSLFWLVFLFSIVTNLLMLSFPIYM
                     LQVYDRVLTTSHADTLLMLTVIVGGALLVMACIDGLRTAMTLRIGGWLVDRLGPVYLA
                     SGVRARLAGNPTSSSLIGDLAELRNFISTQGLTFFCDAPWVPVFVVIIFLIHPYLGIL
                     AASTAFLLFLISIVNDAATRPLLKRAGDMNAIAIRDADATVRNAEIVSSMGMLNPMVE
                     RWQINHFAVLEYLRRAGERGGVLVSLTKFLRLFAQVGVLGLGALLVIQGELTAGAMIA
                     CSILLGRALAPVETALTAWKNFTQARHAYGRLKAQLQAFPTPVTRTRLSDPTGRVSVK
                     RLSFEVNGQNILRGVSFEIEPGDAIAVIGPSAAGKSSLCRFLVGLSAPTDGEVRLDDS
                     VVQHWDPQELGRHIGYLPQYVELFAGSVKENIGRMHIRDDTAVLAAAKLAHAHDMIQR
                     LPEGYDTIIGDGSVGLSAGQRQRIGLARAVFGDPKFIVLDEPNANLDQAGESALAAAI
                     AELKSRGAAIVMVGHRPSTLSEATKILLLKDGRVQVFGPRDEVLATMRERTVAKSKRE
                     EAPAEAPSQVG"
     gene            complement(3564..>4026)
                     /locus_tag="VFY27_05140"
     CDS             complement(3564..>4026)
                     /locus_tag="VFY27_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764932.1"
                     /translation="IDSSKRGDAAMSGNWNWPPVRVFEFQFDKQINIEWNTDLNFDVH
                     VDVDKYVDVDIDVDLDIEDNSTGPSVIDIQDLRTAGAVPNGLIAFSSVSVEDTSSVVQ
                     LQFDTNIYDIIAQASATGTHVNTFAELDMNLLIPATGGFHFAALGVTAVDD"
BASE COUNT          819 a         1206 c         1222 g          779 t
ORIGIN      
        1 tatctacaaa aacaaaattt ccaacagcat ttgcggcaga gtgctgagca ctccgcacaa
       61 ggattttgtg ccaataaggt aacggctcaa cgagccgatg gttcgatcag gaagttccgt
      121 cgtccgctcc gcgcccaaga cgtcgcgctg acattcgccc gcgcgccgct agaccgctac
      181 gggacctccg tcgtggcgat tcaggcctcg cgcagagttt tccagatggc gtcgagaagc
      241 gggcgcagca ggtagttcgc catcgtccgc tcgccggtca caatgagaac atcgaccggc
      301 atgccgggaa ccagctccac gctggcgccg acacgatgca gttcgtcgcg atcgacctcg
      361 acgcgggcaa gatagtaggc cttggacgac tcgtcgacca ttcggtcggc cgataccgag
      421 cgcaccgttc ccgcaatgcg cggcgccgca cgcgaagaaa acgcagtgag ctggatctgc
      481 gcttgtagcc cctgctgaac cacgtcgaca tccgttggag aaatgcgcgc ctcaatgatc
      541 agcttctcat ccagcggaac gatgtcgagg atcgggaccc cgggctgaat gacgccgctc
      601 acggtcttga acttcaggtt gacgacggtt ccggcaagcg gagcggggat cacggtgcgg
      661 gtcaggacat cgccgcttgc cagcaatttc tcgtccagtc cattcagctc cgctcgtacc
      721 tgatcgagct gcgtcgcgat ctggtccgcg cgcgttgcat cgtaggcgag aagctgcaat
      781 tctgcctcgc cgatctgctg ctgtgcttcg gagatcgcgg cctcgtattg gccgagcctg
      841 ccggtcagct ccgcctgcat gcgcaacatt cgtaacagct ccggctttgc cagatagcct
      901 ctttttgcga gttcgcgctt gccgacgatt tcctcatcga gaaagtcgat ctgtctgctt
      961 gcgctcgtga cctgcgcctc aaaccctttg atctgttcgc gcaattgccc catgcgctga
     1021 cgaagaatgc gatttttgga ctcgtgcgac gtttgccgca cgaggaataa ttcctgctgc
     1081 gcggccatca tcgttgtgac gtcggggcct gccgtcgcga gatcgggagg gaattccacc
     1141 tcgtccttgc cggtgcgctc ggcgtcaaga cgcgctttgg tgacgcgcag cgtccgctgt
     1201 tgcttgagca gcagatcgta attggctttc gcctgcacgt tctcaaggac aagcagcggc
     1261 tggccctttt cgacaatgtc gccatcgcgg acactcagcg tgcgaatgat gccgccttcc
     1321 agatgctgga ccgtgcggcg acttccgtcc ggactgatga tgccgggtgc cactgctcca
     1381 ccggcgagcg gaaccgtgcc cgcaaagccg ccgaagccaa ttacaaaaat cccgattata
     1441 tacgcgccaa gtttcgccgg tccgcgcacg gcttccttca aggcgatatg atgcccgaga
     1501 tcgtacacct gcagcgcggt cacccggtcg accgggacaa gttcacggct tccagacata
     1561 gcgcacctca ctggccgagt tcacgtccgt cagcgtccga tcggttatcc aacctgcgac
     1621 ggcgcttctg ccggcgcttc ttcgcgtttc gacttcgcga ctgtcctttc ccgcatcgtg
     1681 gcgagcacct catcgcgcgg gccgaagacc tgcacgcgcc cgtccttcag caggagaatc
     1741 ttcgttgctt ccgacaaagt ggagggccgg tggccgacca tcacgatcgc ggctccgcgc
     1801 gacttcagtt ccgcaatggc tgccgccaac gcggattcgc cggcctggtc gaggttggca
     1861 ttcggctcgt cgagaacgat gaatttcggg tcgccgaaga ctgcccgagc cagtccgata
     1921 cgctgccgct gcccggcgga aaggccgacg ctgccatcgc caatgatcgt gtcatagcct
     1981 tctggcaacc gctgaatcat gtcgtgggcg tgcgcgagct tggccgccgc gagaacggcg
     2041 gtatcgtcgc gaatgtgcat gcggccgatg ttctctttga cggagccggc aaacagctcg
     2101 acgtactgcg gcaggtagcc gatatgccgg ccgagttcct gtgggtccca atgctggacc
     2161 acggaatcat cgagcctcac ttcgccgtcg gttggcgcgg acaatccgac cagaaagcga
     2221 cacaaggagc tcttaccggc ggcggacggg ccaatgacgg ctatggcatc gccgggttcg
     2281 atctcgaacg agacgccgcg cagaatgttt tggccattga cctcaaacga aagcctcttg
     2341 acggatacac gaccggtcgg gtcggacaac cgggtgcgcg tgaccggcgt cggaaacgcc
     2401 tgaagctgcg ccttcaatcg tccataggca tgacgcgcct gggtgaaatt cttccatgca
     2461 gtgagcgccg tctcgaccgg cgccagtgcg cggccaagca ggatcgagca cgcgatcatg
     2521 gcgccggccg tgagctcgcc ctggatcaca agcaaagcgc cgagtccgag aacgcccacc
     2581 tgcgcgaaca ggcgcaaaaa tttggtaagc gagaccagta cgcctccgcg ctcaccggcg
     2641 cgccgaagat actcaagcac ggcgaaatga ttgatctgcc agcgttcgac catcggatta
     2701 agcattccca tggaagagac gatctcggcg ttccgcacgg tagcgtcggc gtcgcgtatt
     2761 gcaattgcat tcatgtcgcc ggcgcgtttc agcagcggtc gggttgcagc gtcattcacg
     2821 atgctgatca ggaaaaggag aaacgcagtc gaagctgcca gtattccaag atagggatgg
     2881 atgagaaaga tgatgaccac gaacaccggc acccagggag cgtcgcagaa gaacgtcagg
     2941 ccctgggtgg agataaagtt gcggagctcg gccagatcgc caatgagtga agaactcgtc
     3001 ggatttccgg cgagacgcgc gcgcacgccg ctggcgagat agaccggccc gaggcgatcg
     3061 acgagccagc cgcctatgcg cagcgtcatt gccgtgcgca agccgtcgat gcacgccatc
     3121 accagcagcg cgccgcccac gatcacagtc agcatgagaa gcgtgtcggc gtgtgacgtg
     3181 gtcagcacgc ggtcgtagac ctgcagcatg tagatcggga acgagagcat cagcagattg
     3241 gtgacgatac tgaataggaa gacaagccag aacagcgagc gacaactggc gatcgcctgc
     3301 tggagttcgg aattcctgct cgggaaagca cggctcattc ggggaccatc gaaaggtgca
     3361 tctctttgac aaattgactt cgaaggcggg gccccttgga ggatagggcc atcaatgctg
     3421 aacgtttcga ttgccggcct caattgacct gtgggtgtac acccataggc gtaggtatct
     3481 atttcctgtt aggtcgaacc ggaaaacaac gaggcggcag cgaattgctg ccgcctctgg
     3541 cgcaaggacg gggtcgcgga cgttcagtcg tccacggcag tcacgcccaa ggcggcaaaa
     3601 tggaagccgc cggtcgccgg gatgagaagg ttcatgtcga gctcggcgaa ggtattgaca
     3661 tgggtgcccg ttgccgaagc ctgagcgatg atatcgtaaa tattcgtgtc gaactggagc
     3721 tggaccaccg aactcgtgtc ctcgacggac acgctgctaa aagcgatgag gccattgggc
     3781 acagcaccgg cggtgcgaag gtcctggatg tcgattacgg agggacctgt cgagttgtct
     3841 tcgatgtcga gatcgacgtc gatgtcgacg tcaacgtact tatcgacgtc gacatggacg
     3901 tcgaagttca gatcggtatt ccactcgata ttgatttgct tgtcgaattg gaattcgaac
     3961 acgcggactg ggggccagtt ccaattgcct gacatcgctg cgtctcctcg tttgctggaa
     4021 tcgatc
//
LOCUS       DASUOM010000247         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1147198,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000247 DASUOM010000000
VERSION     DASUOM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147198"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFY27_05145"
     CDS             complement(<1..472)
                     /locus_tag="VFY27_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5764933.1"
                     /translation="MQYDHITVPAGGEAITVNRDSSLNVPDRPVVPYVVGDGIGIDVT
                     PVMQDVVNAAVSRAYGEKRQIKWMRVYAGAEAAEVYGENVHLPDETLHAFKKFIVSIK
                     GPLTTPVGGGVRSLNVSIRQALDLYACVRPIRYFPGVSTPMKESGLVDMVVFREN"
     gene            complement(571..>2172)
                     /gene="aceB"
                     /locus_tag="VFY27_05150"
     CDS             complement(571..>2172)
                     /gene="aceB"
                     /locus_tag="VFY27_05150"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX5764934.1"
                     /translation="SSAPDYDPNSRPKVEGPVGVASGEILSDGALDFIGALARTFTPR
                     IEQALAAREERQQAIDNGEMPDFLESTRDIRESAWRVCPVPADLEDRRVEITGPTDRK
                     MIINALNSGAKVFMADCEDSLSPTWKNIIQGQVNLRDAVRRSIELDAGAKHYALQEQT
                     ATLIVRPRGWHLLEKHITVDGKPVPGALVDFGLYLFHNAAELKKRGTGPYFYLPKLES
                     HLEARIWNDVFRFAEEQGTVGKGEIKVTVLIETILAAFEIDEILYELKDYIVGQNCGR
                     WDYIFSFIKKFRKYPEFVLPDRSEVTMMTHFLRSYSRLVIKTCHKRGAYAIGGMAAQI
                     PIKNDPEANQRALAKVRADKEREVQDGHDGTWVAHPALVPLAMEIFDAHMPGPNQLDR
                     LRANDRVSAADLLQVVKGKITEAGVRENISVGLQYMTAWLDGNGCVPINNLMEDAATA
                     EISRAQLWQWVHHETGILDEGRNITYEYFRKLLDEEMEKIRGAVGDERFGSGSFRRAA
                     ELFDQITRQEEFAPFLTLVAYEDLE"
BASE COUNT          369 a          691 c          658 g          454 t
ORIGIN      
        1 tgttctcgcg gaagacgacc atgtcgacaa ggccggactc cttcatcggc gtcgagacgc
       61 cggggaaata ccggataggc cgcacgcagg cgtaaaggtc gagggcctgg cggatcgata
      121 cattgagcga gcggacgccg ccgccgaccg gcgtcgtcag cggacccttg atcgagacga
      181 tgaatttttt gaaagcgtgc agcgtctcgt cggggaggtg gacattctca ccgtagactt
      241 ccgcggcctc cgccccggcg tacacgcgca tccatttgat ctgccgcttc tcgccgtagg
      301 cgcgggagac agcggcattg acgacgtcct gcatgacggg cgtcacatcg atgccgatgc
      361 catcgcctac gacgtaggga acgacggggc ggtccggcac gttgagcgag ctgtcgcgat
      421 tgacggtgat cgcctcgccg ccggcgggta ccgtgatgtg gtcgtactgc attccctgcc
      481 ctcttatcga tattggcgta gagggcgcgc attgtaccgg gaaggccggg aatttgtttt
      541 agtgctgccg gcgacagcct gtaggctccc ctactccagg tcctcgtaag ccaccagtgt
      601 caggaatggc gcaaactctt cctgcctggt gatctggtcg aacaactccg cggccctcct
      661 gaagcttcct gacccgaagc gctcgtctcc gacagcaccg cgaatcttct ccatctcctc
      721 atcgagcagc ttcctgaagt attcgtacgt gatgttgcgg ccttcatcga ggatgccggt
      781 ctcatgatgc acccactgcc acaactgtgc cctcgagatt tccgccgtcg cggcgtcttc
      841 catgagattg ttgatcggca cgcagccatt gccgtcgagc cacgccgtca tgtattgcaa
      901 gccaacgctg atgttctccc gtacgccggc ctccgtgatc tttcccttga ccacctgcag
      961 caggtccgcc gccgacacac gatcgtttgc acgcagcctg tcgagctggt tcggccccgg
     1021 catgtgcgcg tcgaagattt ccatcgcgag cggcaccagc gccggatgcg cgacccaggt
     1081 gccgtcgtgc ccgtcctgta cttcgcgctc cttgtcggcg cgcaccttgg ccagcgcccg
     1141 ctggttcgct tccgggtcgt tcttgatcgg gatctgcgcg gccatgccgc cgatggcata
     1201 cgcgcctcgc ttgtggcagg tcttgatcac cagcctcgaa taggagcgca ggaagtgcgt
     1261 catcatcgtc acctcgctcc ggtccggcag cacgaactcg gggtacttgc ggaacttctt
     1321 gatgaagctg aagatgtaat cccagcgccc gcagttctgt ccgacgatgt agtccttcag
     1381 ctcgtacaga atttcgtcga tctcgaacgc ggcgagaatg gtctcgatca gcacggtaac
     1441 cttgatctcg cccttgccca cggttccctg ctcttccgcg aagcgaaaaa cgtcgttcca
     1501 gatccgcgcc tccaggtgcg attcgagctt cggcaggtaa aagtaaggtc cggtgccgcg
     1561 cttcttcagt tccgccgcgt tatggaagag gtacagcccg aaatcgacga gtgcgccggg
     1621 cacaggcttg ccatctaccg tgatgtgctt ctccagcagg tgccagccgc gcggccggac
     1681 gatcagggtc gcggtctgct cctgcaacgc gtaatgcttg gcgccggcat ccagttcgat
     1741 gctccggcgg accgcatcgc gcaggttgac ctgcccctgg atgatgttct tccaggttgg
     1801 cgacagcgaa tcctcgcagt cggccatgaa cactttcgcg ccggaattga gcgcgttgat
     1861 gatcatcttg cggtcggtcg ggccggtgat ctcgacgcgg cgatcctcga gatccgcagg
     1921 caccggacac acgcgccagg cggattcgcg gatgtcgcgg gtgctttcga ggaagtccgg
     1981 catttcgccg ttatcgatcg cctgctggcg ctcctcccgt gctgcgagcg cctgttctat
     2041 gcgcggcgtg aatgtccggg cgagagcacc aatgaaatca agggctccat cgctcagaat
     2101 ttcaccggac gcaacaccga cgggtccctc gactttcggc cgggaattgg ggtcgtaatc
     2161 gggggcgctg ga
//
LOCUS       DASUOM010000248         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1496560,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000248 DASUOM010000000
VERSION     DASUOM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496560"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFY27_05155"
     CDS             complement(<1..683)
                     /locus_tag="VFY27_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5764935.1"
                     /translation="MRFEKHRAMASAIMLAATWLLAAIPAAGAERTRHTVTVDGHPVA
                     LWEKSAVDAEEAILLVHGRTWSAVPDFDLQVEGEDLSLMDGLVEQGYAVFAVDLRGYG
                     ATPRDSSGWLTPQRAATDLARVLEWIAARQEWRKNPHLFGWSMGSTNSLLLAQQQPEL
                     IASLTLFGYWHDLDEKLPADDPGAVPQKLPNSAEAAASDFIMPGSISKKAVDTYVAMA
                     LEADPVKADL"
     gene            complement(799..1392)
                     /locus_tag="VFY27_05160"
     CDS             complement(799..1392)
                     /locus_tag="VFY27_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX5764936.1"
                     /translation="MDDDQDPSNLEQLLDRIGEAPEDEGRVSLDAILDVVGRRSFGPV
                     LLVAGLITLAPIIGDIPGVPTIMGILILLVAGQVLFRHEHFWLPGWVLRRSISRDKLT
                     KALGWLQRPARFIDRLLRPRLTVLTRPIGAYVIAFMCLQIALIMPFMEVVPFSANAAG
                     LVLAAFGLALIACDGVLALIAYATLALSLGIAAYTLL"
     gene            complement(1455..>2301)
                     /locus_tag="VFY27_05165"
     CDS             complement(1455..>2301)
                     /locus_tag="VFY27_05165"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX5764937.1"
                     /translation="HADGALAEGPIALVEVQAYVYAAKQEIADVARALGQHADAARLV
                     AEAEAIRLRFEERFWSEKLGNYVLALDGEKRPCEVRSSNVGHVLFSGIASRERAARVA
                     NDLLSQSFYCGWGVRTIPSGEPRYNPMSYHNGSVWPHDNALIALGLARYGDTNSVQTI
                     FDGLFSAASFMKLRRLPELFCGFRRRPGRGPTNYPVACSPQAWAAAAPLALLQASLGL
                     RFDTVNQAIVMHHPRLPEFLDEVLLRNLKLQKSSVDLLLRRHGNDVAINVLRRSGDVA
                     VDIRA"
BASE COUNT          447 a          748 c          720 g          386 t
ORIGIN      
        1 aggtccgcct tcacggggtc ggcttcgagc gccatcgcga catacgtgtc gacggccttc
       61 ttgctgatcg agccgggcat gatgaaatcg ctggcggctg cctctgccga gttgggaagc
      121 ttctgcggca ccgcaccggg atcgtctgcg gggagctttt catcgaggtc gtgccagtag
      181 ccgaacagcg tgagtgatgc gatcaactcc ggctgctgct gcgccagcag tagcgagttt
      241 gtcgaaccca tcgaccagcc gaacaggtgc ggattctttc gccattcctg tcgtgccgcg
      301 atccattcaa ggacacgggc gaggtccgtt gccgcgcgct gcggagtcag ccagccgctg
      361 ctgtcgcggg gagtggcgcc atagccgcga agatccacgg cgaacaccgc gtaaccctgc
      421 tccaccagcc cgtccatcag cgaaaggtcc tcaccctcga cctgcaggtc gaaatcggga
      481 acggcgctcc aggttcgacc gtgcaccagc agaatggcct cctccgcgtc gaccgccgac
      541 ttctcccaca gcgcaacagg atgaccatcg acggtgacgg tgtggcgggt ccgctcggcg
      601 cccgcggcag gaatcgctgc cagaagccag gtggctgcca gcatgatggc ggacgccatc
      661 gcgcggtgtt tttcgaacct cataatgaaa ttccagcggg gaccaggaga gaagacgcag
      721 gagcatatca ggatcctgag aactctccga accatgcgtg gcgtgcggtg ccgaagaatc
      781 gctaacgctc tcaacgactc acaacagggt ataagcagct atccccagcg acagcgccag
      841 cgtcgcgtac gcgatcagcg ccagcacgcc gtcgcaagcg atcagcgcca ggccgaaggc
      901 ggccagtacc aggccagcgg cgttggcgct gaaaggtacg acttccatga acggcatgat
      961 gagcgctatc tggaggcaca tgaaggcgat cacgtacgcg ccgattggcc gcgtaagcac
     1021 cgtcaggcga ggacgcaaca ggcgatcgat aaaccgtgcc ggacgctgta accatcccag
     1081 tgccttagtc agcttgtcgc gcgagatgga ccgccgcagc acccatcccg gcagccagaa
     1141 gtgctcatgc cgaaacaaca cttgcccggc aaccagcagg atgagaatcc ccatgatggt
     1201 cggaacgccg gggatgtcgc cgataatggg cgccagtgtg atcaggccgg caaccagcag
     1261 gaccggcccg aaagaacgtc gtcccactac gtccaggatg gcgtcgaggg aaacccgccc
     1321 ctcgtcctcc ggtgcctccc cgatcctgtc gaggagttgc tcgaggttgc ttgggtcctg
     1381 atcgtcgtcc acaagaattc cgtctggtcc ggtttttcga gtcccgccga cactctctgc
     1441 gccagcgcat tttgctaggc gcgtatatcc actgcgacgt cgccgctgcg gcgcagcacg
     1501 ttgatggcaa cgtcgttgcc atggcggcgc agcagcaggt caaccgatga tttctgcagc
     1561 ttcaggttgc gcagcaacac ctcgtccagg aactcaggca ggcgagggtg gtgcatgacg
     1621 atagcctggt tcacggtgtc gaagcgcaac ccgaggctgg cctgcagcag cgccagcggc
     1681 gctgccgcag cccaggcttg cggcgagcag gcaacggggt agttggtcgg gccgcggccc
     1741 ggacgccgcc ggaagccgca gaacagttcc ggcaggcgcc gcaatttcat aaagctcgcc
     1801 gcactgaaga gaccgtcgaa aatcgtctgc accgagttcg tgtcgccata gcgcgccagc
     1861 ccgagggcga tcagcgcgtt gtcgtgcggc cacaccgacc cgttgtgata ggacatgggg
     1921 ttgtaacggg gttcgccgga aggaatggtg cgcacgcccc agccgcaata gaagctctgg
     1981 ctcaggaggt cgtttgccac ccgcgctgcc cgctcgcgcg atgcgattcc tgaaaacagc
     2041 acgtgcccca cgttcgaact ccgcacctca cagggacgct tttcaccgtc gagcgccagc
     2101 acgtagttac cgagcttttc ggaccagaat ctttcctcga agcgcaggcg aattgcctcc
     2161 gcctcggcga cgagccttgc cgcgtccgca tgctggccca gagcgcgcgc aacgtcggcg
     2221 atttcctgct tcgccgcata tacgtacgcc tgcacctcga cgagagcgat gggtccctcg
     2281 gcgagcgccc cgtcggcatg g
//
LOCUS       DASUOM010000249         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1496372,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000249 DASUOM010000000
VERSION     DASUOM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496372"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..2333
                     /locus_tag="VFY27_05170"
     CDS             18..2333
                     /locus_tag="VFY27_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX5764938.1"
                     /translation="MRIRGFGEGRLNKVYPLDVTSGYPRGSGLPPPSAPDGEDVITIQ
                     RVIRAVRRRLALMVAVFLVTFSAVAMFTFQLKPMYTATSRVIVNTRDQNIVDVSAVLS
                     GMPANAAAVDTEAQILRSRILIEKLVRRLDLVNDAEFNWRKAEPSDMDKRVNAAKSFI
                     RSLVPFGGEAPVEQAPQIDPVEAERAEMDDVVGAVRGAVSVNRLGSTFIIEISVRSAN
                     PKKAALLANTLAEVYLDNQLDTKYEATRRAQAWLDERVAQLREEVRAAESAVEAHRAA
                     SGLLRPGGDQTLVEQAIRDLNASLIVAQAEYSEKTARLRNLNSQMQAGAGIDSIAEAQ
                     NSTAIASLRTRQADIAVRKAAAEGRYEPRHPEYQRVQVEAADVQRQIDAELRRISSSL
                     EQEVLIAREKVNTLQASLNQAKGELATNSRAGVEQAALEREAMASRNLFEEFNNRFKE
                     TDHLSQITDPDAVIDSYAPIPWAPSFPNTNLNLMLGMMLGLALAGFAGLVVELLDSYL
                     SSPEDVEQVAGVPYIGQIPLLPAAGNFTKARIKPAEYLLEKPHSGFAEAYRHLRASIM
                     FADIDKAAKTVAVVSSLPSEGKTSMTYCLGRMAAMSGTKTIVIDGDIRLRQLTEVSGL
                     KPEAGLLEYLFGETRLADAVVTDEKTGLHILPLTDRKHTPRDVFGSRAFDALLSMLQQ
                     TYDLIIIDTGPILLMAETRVVTSKVDQVVVATRWRKTNRGTLRETMKILRDFRANVAG
                     VVLTFVDLRKRAHHAYTSADYKAYAKYYHEH"
BASE COUNT          472 a          797 c          732 g          387 t
ORIGIN      
        1 cctgacgctc gcgttgcgtg aggatcaggg gcttcggaga gggacggttg aacaaggttt
       61 atccgctgga cgtcacgtcg ggatatccgc gtggctcggg tcttccgccg ccctccgcgc
      121 cggatggcga ggacgtcata accatccagc gtgtcatccg ggcggtccgg cgccggctgg
      181 cgctgatggt ggccgttttc ctggtcacct tctccgctgt ggcgatgttc acgttccagc
      241 tgaagcctat gtacaccgcg acctcccgcg taatcgtcaa cacacgcgac cagaacatcg
      301 tcgacgtcag cgcggtcctc tcaggcatgc ctgccaacgc agcggccgtt gacacggaag
      361 cgcaaattct tcgcagccgg atcctcatcg agaagctggt gcgccggctc gacctggtca
      421 acgacgccga gttcaactgg cgcaaggccg aacccagcga catggacaag cgcgtcaacg
      481 cggcgaagag cttcatcaga agtctcgttc cgttcggagg cgaagcgcct gtcgagcagg
      541 cgccgcagat cgacccggtc gaagccgagc gggcggagat ggacgatgtc gtcggcgcgg
      601 ttcgcggagc agtctccgtc aaccgcctgg gttcgacctt catcatcgag atcagcgtcc
      661 gcagtgcgaa tccgaagaag gccgccttgc tggcgaacac gctcgcggaa gtctatctcg
      721 acaaccagct cgacacgaaa tacgaggcga cccgccgggc gcaggcctgg ctggacgaac
      781 gcgtcgccca gctccgggaa gaggttcgcg cggccgaaag cgccgtcgaa gcgcaccgtg
      841 ccgcctccgg tctgctgcgt cctggcgggg accagacgct tgtcgaacag gccattcgcg
      901 atctgaatgc cagcctgatc gtcgcccagg ccgaatactc cgagaagacc gcgcgtctcc
      961 gcaacctcaa ttcccagatg caggcgggcg ccggcatcga cagcatcgcc gaggcgcaga
     1021 actccacggc gatcgcctcc cttcgtacgc ggcaggctga catcgccgtt cgcaaggccg
     1081 ccgcagaagg ccgctacgag ccgcgccacc cggagtatca gcgcgtccag gttgaagcgg
     1141 cggacgtcca gcgccagatc gacgcggaac tgcgacgcat ctcatcctcg ctcgaacagg
     1201 aagtcctgat cgcgcgcgag aaggtgaata cactgcaggc gagcctcaac caggctaagg
     1261 gcgaactggc caccaacagc cgcgcaggcg tcgaacaggc cgcgctggag cgcgaagcga
     1321 tggcaagccg caacctgttc gaggagttca acaaccgttt caaggaaacc gatcacctta
     1381 gccagatcac cgatccggat gccgtgatcg acagctatgc tccgattccg tgggccccga
     1441 gctttcccaa caccaacctg aacctgatgc tgggcatgat gctcggcctg gcgctcgcgg
     1501 gctttgccgg tctcgtggtc gaattgctcg acagctatct ttcctcgccc gaggacgtcg
     1561 agcaggtggc cggcgtaccc tatatcggcc agatccctct gctgccggcg gccggcaact
     1621 tcaccaaggc gcggatcaag cccgcagaat acctgctgga aaagccgcac tccggctttg
     1681 ccgaagccta tcggcatctg cgcgcctcga tcatgttcgc tgacatcgac aaggcggcga
     1741 aaacggtggc ggtcgtttcc tccctgccca gcgagggcaa gacgagcatg acctactgcc
     1801 tcggccgcat ggccgcgatg tcgggcacca agacgatcgt catcgacggg gacatccgcc
     1861 tgcgccagct cacggaggtg tcgggcctga agccggaagc cggactgctc gaatacctgt
     1921 tcggggaaac caggctcgcc gacgccgtgg tcaccgacga gaagacaggg ctgcacatcc
     1981 ttccgcttac cgaccgcaag catacgccgc gcgacgtgtt cggcagccgg gcgttcgacg
     2041 ccctgctcag catgctgcag cagacctacg acctgatcat catcgacacc gggcccatcc
     2101 tgctgatggc cgagacccgt gtagtcacct ccaaggttga ccaggtcgtt gtcgcgacgc
     2161 gctggcgcaa gaccaaccgc ggcacgctgc gggagacgat gaagatcctc cgagacttcc
     2221 gcgccaacgt cgccggtgtc gtgctgacct tcgtcgatct gcggaaacgg gcgcatcacg
     2281 cctacacgtc ggccgactac aaggcgtacg caaaatacta tcacgaacac tagagactca
     2341 gcgtccgacc gggctcggaa aaggtcctcc ggaatttttc ggaatcga
//
LOCUS       DASUOM010000250         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_544736,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000250 DASUOM010000000
VERSION     DASUOM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544736"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VFY27_05175"
     CDS             <1..1578
                     /locus_tag="VFY27_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5764939.1"
                     /translation="VAACKAMASLPDPLSTLLQPRAYPHAVRDVQLVETHMSWVLLTG
                     EFAYKIKKPVNYSFVDLRSPERRAFYCREELRLNRRFTRNLYIEVCEITVTDGTASIA
                     GQGEAVDHAVRMRQFEASQELDRLLADSRIEPVELDSFGRELAAIHRQLPVDQGPDAA
                     GRPETTRKILLENLEQCQQGAALLGTDDVMRALRQPYVVRVAEADEWLERRWQQGHVR
                     ECHGDLHSRNVVKYGGRLVAFDCIEFDPSFRWIDVADDVAFLMMDLGARQFPLHAHAF
                     RSGYLAESGDFAACRMLRLYQTHRALVRAKVTALEAAGKEGSGRESALEQHRGYLRDA
                     EQQLAVRQPMLLLMFGLSGSGKTWLAERLARPLDAVHIRSDVERKRLAGLAEQQQSHS
                     GLGRDLYSPGKSAEMYERLADCAGHALAGGYTAIVDAAFQRRSDRARFKTLAAQYGCE
                     LRVIHCHAPRVELETRLADRSRARRDASEADREVLAWQERHLEPLQVSEGLATVDADT
                     TAENVADDVRHSLLRNP"
     gene            1693..2070
                     /locus_tag="VFY27_05180"
     CDS             1693..2070
                     /locus_tag="VFY27_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5764940.1"
                     /translation="MKYLCLACEEESKLNALSRKEWDALRTATLKYVEELRENGHLIV
                     AEPLQSASTAVSVRVRNGTVTTTDGPFAETKEQVGGIFMIDAESLDEALEIAAGWPSA
                     QIGTIEVRPIEPELSAEGRYAKL"
     gene            complement(2100..2822)
                     /locus_tag="VFY27_05185"
     CDS             complement(2100..2822)
                     /locus_tag="VFY27_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEX5764941.1"
                     /translation="MVYRACRRRCCRSPDQVRIPVPLTLTANEVRVIGCLMEKSVFTP
                     EQYPLTLNALTNACNQKSGRNPVMSLQPGVVQRTVRDLEGKHLVRVEENFKSRVEKYT
                     QRFCNTRFSDYQFEPPQFAVVCLLLLRGAQTPGELRARSGRLHAFPDNDAVVDALKSL
                     VDREGGPLVVKLPRTAGRKDSEYMHLFGGAVDLQQHARDSEAQALDRSRDRVSVQELA
                     ERVAALESEVASLRDLLESKGA"
     gene            complement(2860..>3637)
                     /locus_tag="VFY27_05190"
     CDS             complement(2860..>3637)
                     /locus_tag="VFY27_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764942.1"
                     /translation="TWLVDGSTRSQRERAKLNWAPGPASVNVQWAGNAAAFDNSVALT
                     CDFQSTVLLPTHEASGHSESLLLSGRDCMLPEEGSSHAGIEAQAWGMSYTWDRPKRQS
                     EALLAVIDPRWAGDGDLAGVGPGYELGLRHRRTIGTLSASALVSLRRAPALDNAVAGP
                     VLTEDTEWAANASLTWQRPEASLSASWATGVDLFWFTPEAGQQSDQFGLALNLSRWIE
                     FLMPRSSPQLAMNWNWSEIRLPSAETIGSNSLRLDVALMF"
BASE COUNT          701 a         1114 c         1177 g          645 t
ORIGIN      
        1 gtggcagcat gcaaggccat ggcttcgctc cccgaccctc tctcgacgct tctccagccc
       61 cgggcatacc cgcacgccgt gcgcgatgtg cagctcgtcg aaacgcacat gtcgtgggtc
      121 ctgctgacgg gggagttcgc ctacaagatc aagaagccgg tcaactattc gtttgtcgac
      181 ctgaggtcgc ccgagcggcg cgccttctat tgcagggaag aactgcgcct caaccggcgc
      241 ttcacccgca acctctatat cgaggtctgt gagatcaccg tcaccgacgg cacagcgtcg
      301 atcgccgggc agggagaggc ggtcgaccat gcggtgcgca tgcggcagtt cgaagcatcg
      361 caggaactgg accggctgct ggccgacagc cgaatcgaac cggtggaact ggactccttc
      421 ggcagggagc ttgcagccat ccatcggcag cttcctgttg accaggggcc cgatgcggcc
      481 ggtcgtcccg aaacgacgcg gaaaatactg ctcgagaacc tggagcagtg ccagcagggt
      541 gcggcgttgc tcggcaccga cgatgtcatg cgggcgctgc gacaacccta tgtcgtgcgc
      601 gtggccgagg ccgacgaatg gctggagagg cgctggcagc agggccacgt ccgggagtgc
      661 cacggtgatt tgcattcgcg caacgtcgtg aaatacggtg gccggctcgt cgccttcgat
      721 tgcatcgagt tcgacccctc attccgctgg atagatgtcg ccgacgatgt ggcgttcctg
      781 atgatggacc tgggagcgcg acagtttccg ttgcacgcgc acgcatttcg ctcaggctat
      841 cttgccgaaa gcggggattt tgccgcgtgc cgcatgctgc gtctctacca gactcaccgc
      901 gctttggtac gtgccaaggt gactgcactg gaagcggcag gcaaagaggg gagcggaagg
      961 gaatccgctc tggagcagca ccggggctac ctgcgcgatg cggagcagca gctggcagtc
     1021 cggcagccta tgctcctcct gatgttcggt ctttcaggct ccggaaaaac ctggctggct
     1081 gaaaggctgg cgcggccact cgatgcggtt cacatccgct cggacgtgga gcgcaagcgc
     1141 ctggcgggac tcgcggaaca gcaacagtct cattccggcc tgggacggga cctttactcg
     1201 ccggggaaga gcgcggagat gtatgaacgg ctcgccgatt gtgccggtca cgcactcgcc
     1261 ggcggctata cggcgatcgt tgacgcggcc ttccagcgac ggtcagaccg cgcgcggttc
     1321 aagacgctcg cggcgcagta tggctgcgag ctgcgagtca tccattgcca tgctccgagg
     1381 gtggagctgg aaactcgcct ggctgatcga agccgcgcac ggagggacgc atcggaagca
     1441 gaccgcgagg ttctcgcgtg gcaggaacgg cacctcgagc cgctgcaggt gtcggaaggc
     1501 ctggcgacgg tcgacgccga cactacagcg gagaacgtcg ccgatgacgt acgccatagt
     1561 cttttgcgaa atccgtagcg tcagatacga aaggtctgat cacgcggacc ctgtcgatgt
     1621 acgcggggcc ggttcgacta caatgaaagt cgtcgtggac gactgccttc ggacaaggga
     1681 gttagggaag tcatgaaata cctttgcctc gcgtgcgaag aggagagcaa gctcaatgcg
     1741 ctgtcgcgaa aggagtggga cgcactcagg acggcgacgc tcaagtacgt cgaggaactg
     1801 cgggagaacg gtcacctcat agtggctgag ccgctgcaaa gcgcgagcac ggcggtatcg
     1861 gtcagggttc gtaacggcac agtgacgacc acggacggcc cgttcgcgga aacaaaggaa
     1921 caggtgggtg gcattttcat gatcgacgcc gagagcctgg acgaagcact ggaaattgcc
     1981 gccggatggc cgtccgcgca aatcggcacg atcgaggtga ggccgataga gccggaattg
     2041 agtgccgagg gacgctacgc gaaactttga ggagtggttc gcggcgacga gcaacgatgt
     2101 caggctcctt tcgactccag caggtccctg agcgacgcga cttccgactc gagcgctgca
     2161 acccgctccg cgagttcctg tacgctgacg cggtcacgcg acctgtccaa cgcttgagcc
     2221 tccgaatctc tcgcgtgttg ctgaaggtcc acagcaccgc caaaaaggtg catgtattcc
     2281 gagtcttttc gccccgctgt gcggggaagc tttaccacta gcggtccccc ttcccgatca
     2341 accaggctct taagcgcatc caccacggca tcgttatcgg ggaaggcgtg caaacggcca
     2401 ctgcgtgccc gcagctcgcc gggagtctgt gctccccgca ggagtagcag gcagacaacc
     2461 gcgaactggg gcggctcgaa ctggtaatcg ctgaagcgcg tgttgcagaa ccgctgagtg
     2521 tatttctcca cgcgactttt gaagttttcc tcgacgcgca ccaggtgctt gccttcaaga
     2581 tcgcgcaccg tgcgctgcac cacccccggc tgcagggaca tcacgggatt gcgtccggat
     2641 ttctggttgc acgcgtttgt cagcgcattg agggtgagcg gatattgctc cggggtgaag
     2701 accgacttct ccatcaggca tccgatcacg cgtacttcgt tggcagtcaa ggttaacggc
     2761 acggggatcc tcacttgatc aggactgcgg cagcagcggc gccggcaggc acgatacacc
     2821 atcctgctac cggtctcctg caaatgctcc gtggaagctt cagaacatca atgcgacgtc
     2881 cagcctcagg gaattgctgc cgattgtttc cgcgctgggc aggcgaattt ccgaccagtt
     2941 ccagttcatc gccagttggg gagaggaacg cggcatgagg aactcgatcc agcgtgacag
     3001 gttcaatgcg aggccgaact ggtcgctctg ctggccagcc tccggcgtga accagaaaag
     3061 atcgacaccg gtcgcccagc tggcggacag ggatgcctcc ggccgttgcc aggtgaggga
     3121 agcattggct gcccattccg tatcctcggt cagcaccggc ccggcgaccg cgttatcgag
     3181 cgccggagcg cggcgcagcg agacgagggc actggcgctc agcgtgccga tcgtgcgccg
     3241 gtgccgaagg cccagctcgt aaccgggtcc gacaccggcc agatcaccgt cgccggccca
     3301 ccttggatct atcaccgcca gcagagcctc gctctggcgc ttcggacgat cccaggtgta
     3361 gctcatcccc cacgcctgcg cttcaatccc cgcgtgggag ctgccttcct ccggcaacat
     3421 gcagtcacgc ccggaaagca gcagcgattc cgaatggccg gacgcctcgt gggtcggcaa
     3481 gagaacagtg ctttggaaat cgcaggtcaa ggcaaccgaa ttgtcgaatg cggcggcatt
     3541 gccggcccac tgcacgttta cgctagcggg gccgggagcc cagttcagtt tggcgcgctc
     3601 cctctgcgac cgggtggagc catcgaccag ccaggtg
//
LOCUS       DASUOM010000251         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_984459,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000251 DASUOM010000000
VERSION     DASUOM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984459"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..861
                     /locus_tag="VFY27_05195"
     CDS             295..861
                     /locus_tag="VFY27_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX5764943.1"
                     /translation="MNRNRRALWATTGAAVVLMSLAAQPALADGYAYGRDRAVYDYAR
                     VLSVQPNVRYVTVRSPVQECWEETRTYAVEHRPAGNAGGTIVGAILGGVVGHQFGSGR
                     GNDAATVAGTLLGAAIGNGAGKRHVPYATHYERPVRRCETRYTSREEERIDSYRVIYT
                     YNGRKYATDTPFDPGNRLRIRVDVRPAP"
     gene            966..1298
                     /locus_tag="VFY27_05200"
     CDS             966..1298
                     /locus_tag="VFY27_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764944.1"
                     /translation="MQLEVRSFMRKLTKLMLTATMVGICTTAGASDKFRAADSAYVAA
                     GEHLLIAQGNCASLSEAVERVRRQYNGRIVSAETRISGNREVHHIKVLTSDGKVKTVT
                     VPGCPLRS"
     gene            1306..1998
                     /locus_tag="VFY27_05205"
     CDS             1306..1998
                     /locus_tag="VFY27_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5764945.1"
                     /translation="MRLLVIEDDETLRESLCRQLAEQGFGIERAGNGREGLYFALEYP
                     VDLAIVDLGLPEISGLDLIRELRERKKTYPILVLTARDRWQDKVDGLAAGADDYVVKP
                     FHFEEVLARVNALLRRSGGWASPTLSAGPVTLDTARQELSVDGKVIELTGFEYRIIEH
                     LMMRAGEVISKSELTDRLYDQDFERDSNVIEVFIGRLRKKMDPENRIKPIETLRGRGY
                     RFALERNQSGQQ"
     gene            1995..3356
                     /locus_tag="VFY27_05210"
     CDS             1995..3356
                     /locus_tag="VFY27_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5764946.1"
                     /translation="MSVPAAGSSLSGRLLLSVSVLLLFFFGATIVVLDVAFRETGEQA
                     QRHILDGHLMSLLAAAEPESNGASLRLPPDLPEPRFGNPGSGLYGELRDARGNVVWRS
                     RSAVGVDLPHTAPPTQGSSHYAEIALEDGTELMSLSLSVAWEMPNGDLAPHTFSVAES
                     LDAYYAQIAAFRRRLLGWFAAVAIVMLFSISLVMRRLLQPLRQIEREISEIEEGRRQA
                     LSESLPSELHGVARNVNLLIGNERARSERYMLTLDNLAHSLKTPLAAVRSVINEHPES
                     DLKKRVETQVDRMNDIVRYQLRKPASYASEGFGLTAVAVEKELKRLVEGLLKVYKDKS
                     PDIVIDVAHGAVFRGDRGDFLELAGNLLDNACKWCRSRVELHMRPRGVTGIELTVADD
                     GPGIPEHMAGILLERGMRLDESAPGHGIGLAVVRDIAASYGGEIRIGKSTLRGAEVTV
                     TTA"
     gene            complement(3491..>4010)
                     /locus_tag="VFY27_05215"
     CDS             complement(3491..>4010)
                     /locus_tag="VFY27_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5764947.1"
                     /translation="VNAVIYFRIVDPEKAVIRVANVFEATSQLSQTTLRSVLGQHELD
                     DMLAERDKLNADIQNILDQRTDNWGIKVANVEIKHVDLDESMIRAIAQQAEAERSRRA
                     KVINAEGEKQAADRLAEAARILGGEKQALQLRYLQTLKEVATERSNTIIFPLPLDLIE
                     PLMSLRKSADRE"
BASE COUNT          757 a         1210 c         1294 g          749 t
ORIGIN      
        1 tggcatccgc gaaccgcaag tttgaagcgc gctttcgccg catggagaag gacatccacg
       61 ccctgggtct cgacctggcg gcgctggaca tcgataccct ggagcagcac tggcagcagg
      121 cgaagctcag ggagaggtag ccgggatccg ccatgggacg ggccaactgt gcggcgtatc
      181 acgtctggcg ccaatcagga gccgcattca gccacggttc atggccgcac ccctatcgtt
      241 caccgcaagc ccggagacac ttccgggcgt tgggactgga tggggagaag gacgatgaac
      301 aggaacagac gtgcactctg ggcaacgacc ggcgcagccg tagtgctgat gtcacttgcc
      361 gcccagccgg ctctggcaga cggttatgcc tatggcaggg atcgcgcagt ctatgactac
      421 gcgcgcgttc tgagcgtgca gcctaacgtg cgttacgtga cggtgcggag ccccgtgcag
      481 gagtgctggg aagagacgcg cacctatgcg gtcgaacatc gcccggcagg gaacgcgggt
      541 ggcacgatcg tcggcgccat cctgggcggc gtggtaggtc accagttcgg cagcgggcgc
      601 ggcaatgacg ctgcaaccgt ggccggaact ttgcttggcg ccgcgatcgg caacggcgct
      661 ggcaaacggc acgtgccgta cgcgacccat tacgaacggc cggtgcggcg ctgcgagacg
      721 cgatatacgt cccgtgagga ggagcgaatc gacagctacc gcgtcatcta tacctataac
      781 ggccgcaagt acgcgaccga cacgccgttc gacccgggca atcgactccg catccgtgtg
      841 gacgtgcggc cggcgccatg agctaaggcc caccgttttc tggcggcgga ggccgggcgc
      901 gattgtgact gccctcgttg caatcgcccc ggcaacccac caaacttgcc gcggattcac
      961 acttgatgca gctcgaggtt cggtctttca tgcgtaagct gacgaagctg atgctcacgg
     1021 caacgatggt cggcatttgc accacggctg gcgcaagcga caaattcaga gcggcggata
     1081 gcgcttacgt tgcggccggc gagcacctgc tgattgccca gggcaactgc gcatcactga
     1141 gcgaggctgt ggaacgtgtc cggcggcaat acaacggccg catcgtcagc gccgagaccc
     1201 gcatcagcgg caatcgcgaa gtgcaccata tcaaggtgtt gaccagtgac ggcaaggtca
     1261 agaccgtcac cgtccccgga tgcccgctgc gttcctgagg catccatgcg gctgctcgta
     1321 atcgaagacg acgaaaccct gcgcgagtcg ctctgcaggc agctcgccga gcagggcttc
     1381 ggcatcgagc gggccggcaa tggacgcgaa gggctctact tcgcgctgga atatcctgtc
     1441 gacctcgcga tcgttgatct cggcctgccg gagatttccg ggctcgacct gatccgcgaa
     1501 ctacgggaaa ggaagaagac gtatccgatc ctcgtcctga ccgctcgcga tcgctggcag
     1561 gacaaggtcg atgggctggc ggcgggcgcc gacgattacg tcgtcaagcc gtttcatttc
     1621 gaggaagtgc tggcacgcgt gaacgcgctg ctgcgtcgca gcggcggatg ggcttcccca
     1681 acactgtcgg ctgggcccgt gacgctggac accgccaggc aggagctttc ggtggacggc
     1741 aaggtaatcg aactcaccgg tttcgagtac cgcatcatag agcacctgat gatgcgcgcc
     1801 ggtgaagtga tttcaaaatc cgaactgacc gatcggctct atgaccagga cttcgaacgc
     1861 gacagcaacg ttatcgaagt attcataggc aggctgcgca agaaaatgga tccggaaaat
     1921 cgcatcaagc caatcgaaac gctgcgaggg cgcggttacc ggtttgccct ggagcgaaat
     1981 cagtcagggc aacaatgagc gttccggcag cgggctcatc gctcagcggg cggctgctgc
     2041 tgtcggtgag cgtgctgctt cttttctttt tcggcgccac gatcgtggtg ctggacgttg
     2101 cgtttcgcga aacgggcgaa caggcacagc ggcacatcct cgacggccac ctgatgtcgc
     2161 tgcttgctgc agcggagcct gaaagcaacg gcgcgtccct tcgattaccg ccggaccttc
     2221 ccgagccgcg tttcggcaac ccaggctccg ggttgtacgg ggaacttcgc gatgctcggg
     2281 gcaacgtcgt gtggcgttca cgatcagcgg tcggtgtgga ccttcctcat accgcgccgc
     2341 cgacccaggg cagcagccac tatgccgaga tcgcgctcga ggacggtacg gagttgatga
     2401 gcctgagcct ttcagtagcc tgggagatgc cgaatggcga cctcgcgccc cacacgttca
     2461 gcgtcgcgga aagcctcgac gcctactacg cgcaaatcgc ggcgttccgg cgtcggttgc
     2521 tgggatggtt cgccgccgtc gcaatagtca tgctgttctc gatttcgctg gtcatgcgac
     2581 ggctgctgca accgctgcgg cagatcgagc gggagatatc cgagattgag gagggtcggc
     2641 gccaggcctt gtcggaaagc ctcccttcgg aacttcatgg tgtggcgcgc aacgtcaact
     2701 tgctgatcgg caatgagcgc gcgcgctcgg aaagatacat gctcacgctc gacaaccttg
     2761 cgcacagcct caagacgccg cttgcagccg tacgttcggt catcaacgag catccggagt
     2821 ccgatctcaa gaagcgggtg gagacccagg tcgaccggat gaacgacatc gtccgctacc
     2881 agttgcggaa accggcgagc tacgcatcgg aaggtttcgg cctcacggcg gttgccgtcg
     2941 agaaggagct gaaacggctg gttgaaggcc tgctgaaggt ctacaaggac aagagcccgg
     3001 acatagttat cgacgttgcc cacggggcgg tcttccgcgg tgatcgcggc gactttctcg
     3061 aactcgccgg gaatctcctc gataacgcct gcaagtggtg caggtcgcgc gtggagttgc
     3121 acatgcgtcc gcgcggcgtc acgggcattg aactgaccgt tgcggacgac gggcccggaa
     3181 tcccggagca catggcaggc atcctgctgg aacgcgggat gcgcctcgac gaatctgcac
     3241 ccggacacgg tatcggcctg gccgtggtgc gcgacatcgc cgcttcctac ggtggcgaaa
     3301 tacgcatcgg gaagtcgacc ttgcgcggcg cggaagtcac cgtgaccacc gcgtgagaaa
     3361 tagtcatgta ggagccggcc atgccggcga gttcgctggt tcgtatcgca ctcgcccgca
     3421 ttcgccgtcc gaaagctact gccagagaaa gcccaggggg atttcccctg gcgtgggctc
     3481 ctacaagcgc ctattcgcgg tcggcagatt tacgcaggct catcagcggt tcgatgaggt
     3541 cgagtggaag cgggaagatg atcgtgttgc ttctctcggt cgccacttct ttcagcgtct
     3601 gcaggtaacg aagctgtaat gcctgcttct cgccgccaag gatgcgcgct gcttcggcga
     3661 ggcggtcggc tgcctgcttt tcgccctcgg cgttgatcac tttcgcgcgc cgtgaacgtt
     3721 ccgcttctgc ctgttgcgcg atcgcacgga tcatgctctc gtcgaggtct acgtgcttga
     3781 tctcgacatt cgccaccttg atgccccagt tgtcggttct ctggtccagg atgttctgta
     3841 tgtccgcgtt gagcttgtcg cgttccgcaa gcatgtcatc gagttcgtgc tggccgagca
     3901 cggatcgcaa ggtcgtctgc gagagctggc tggtcgcttc gaacacattc gctacgcgga
     3961 taaccgcctt ctcgggatcg acgatgcgaa agtagatgac ggcgttgacc
//
LOCUS       DASUOM010000252         4245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1719318,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000252 DASUOM010000000
VERSION     DASUOM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719318"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFY27_05220"
     CDS             <1..340
                     /locus_tag="VFY27_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEX5764948.1"
                     /translation="IDSAAGVTILGSVGLVATAMITRKDFSFLRGLLVWAFILAMVGI
                     VASLLFGFELGTWFSVAMIGFAGAAVLYDTSNIIHHYPEDKYVAASLALFASIALMCW
                     YVLRLFMSRD"
     gene            complement(388..1671)
                     /gene="serS"
                     /locus_tag="VFY27_05225"
     CDS             complement(388..1671)
                     /gene="serS"
                     /locus_tag="VFY27_05225"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5764949.1"
                     /translation="MIDPKQLRQSTEEVAANLSRRGIEFDVAAYRGLDERRKALQTRV
                     ESLRNEKNTSAKAIGQAKARGEDVQARLASVKDKGERLEAAERELQEVVQELEAVELG
                     LPNLLADDVPFGRGEAGNVEVRRFREPTSFSFEVRDHVDLGARLKMLDLEVASRMSGS
                     RFTVLRGGLAHMQRALVQFMLDVHTREHGYEETYVPYLVQADALTGTGQLPKFEQDLF
                     RTEGKPPFYLIPTAEVPLTNLVRESILDPDSLPRKLVAHTPCFRSEAGSYGRDTRGMI
                     RQHQFEKVELVQIVAAGESGEALEALTGHAEVILQRLELPYRVVELCSGDIGFGAAKT
                     YDLEVWLPGQSAYREISSCSNFTDFQARRMQARRRNPDTGRPELLHTLNGSGVAAGRA
                     LVAVMENFQQADGSIRVPPALLPYMNGTTVLTPFA"
     gene            complement(1664..3046)
                     /locus_tag="VFY27_05230"
     CDS             complement(1664..3046)
                     /locus_tag="VFY27_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5764950.1"
                     /translation="MPEVNDLFSSGDQRKQAGRPLADRLRPASLDDFFGQRHLLDGGK
                     PLRMAIEQGRAHSMIFWGPPGTGKTTLARIIAASTDSHFIGLSAVMAGVKDIRAAVAE
                     ARQHREMSGRATILFLDEVHRFSKSQQDAFLPFVEDGTLTFIGATTENPSFELNNALL
                     SRARVYLLKPLDADSLMQIVRRALERSAQAFDSRIIRADEDALRLIVAAADGDARRAL
                     NLVELAADLAPEAEGEQRIDLAVAEAVASGGRRRFDKRGEYFYDQISALHKSIRGSDP
                     DASLYWLMRMLDGGCDPLYVSRRMVRVASEDIGNADPRALRITLDAWDALERLGSPEG
                     ELALAQAVVFLACAAKSNAVYKAANAAMADARESGSLEVPMHLRNAPTRLMKDLGYGK
                     DYRYAHDEQDAFAAGETYFPDDMPPRRYYDPVPRGLELQIREKLARNAELTQRISDNK
                     RTGKEKNKDD"
     gene            complement(3086..3490)
                     /locus_tag="VFY27_05235"
     CDS             complement(3086..3490)
                     /locus_tag="VFY27_05235"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5764951.1"
                     /translation="MLPLRHARRWQLAGIAVLAIVLAATLLPAIWFMQEMRDPRIAHS
                     DKWFHAITFMLLTIWFSGQYARRSYWRIAAGMLVFGALIELCQRMLTTYRSAEILDLV
                     ADAVGILAGLLIAWAGVGGWSLKVERWIAARD"
     gene            complement(3499..>4245)
                     /gene="lolA"
                     /locus_tag="VFY27_05240"
     CDS             complement(3499..>4245)
                     /gene="lolA"
                     /locus_tag="VFY27_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX5764952.1"
                     /translation="EAGPTRLPEGRARSLASTVARYTKRDPDPMPVMNTTLGRLVAAS
                     FFLAGASAGAAEPPEVNGEQLIENFVESVRTMRGRFEQSLVDANDTIVESSSGTFEIR
                     RPGQLRWSYEQPYEQLLVADGLNVWSYDVDLAQVTVKPQAEVLSSTPALLLGGAHDVL
                     EDFTYDGSFEDRGTEWVRLLPRDPDSNFESVELGFSDGTLTRMIFADNLDQTTLVAMH
                     DVVLNESIDPERFRFVPPEGVDVVGTPLKD"
BASE COUNT          772 a         1364 c         1282 g          827 t
ORIGIN      
        1 catcgacagc gccgcaggcg tcacgatact cggcagtgtc gggctggtcg cgacggccat
       61 gatcacccgc aaggacttct cgttcctgcg cggattgctc gtctgggcgt tcatcctcgc
      121 gatggtcggc atcgtcgcga gcctgctttt cggcttcgaa ctcgggacat ggttttccgt
      181 tgcgatgatc ggtttcgccg gagcggccgt gctttacgac acttccaaca tcatccacca
      241 ctacccggaa gacaagtacg tcgctgcatc cctggccctg ttcgcctcga tcgcgctgat
      301 gtgctggtac gtgctgcgcc tgtttatgag ccgcgactga actgaaccag tttccgcgcg
      361 gcggaattct gggcgggctc ctgcaagtca ggcgaaaggc gtcagcaccg tggtgccgtt
      421 catgtaaggc agcagcgccg gcggcaccct gatgctgccg tcagcctgct ggaagttctc
      481 catcacggcg accagcgccc ggcccgccgc gacacccgaa ccgttcagcg tatgcaagag
      541 ctccgggcga ccggtgtccg ggttgcgccg gcgcgcctgc atccggcgcg cctggaaatc
      601 cgtgaagttg ctgcacgagg agatctcgcg atacgcggac tgccccggca gccagacctc
      661 gaggtcatag gtctttgccg cgccgaagcc gatatcgccg gaacacagct ccaccacgcg
      721 gtaaggcagt tcgaggcgct gcagaatcac ctcggcatgt cctgtgagcg cctccagtgc
      781 ttccccggac tcgcccgctg ctacgatctg cacgagctcg accttttcga actggtgctg
      841 ccggatcatg ccgcgggtgt cacggccgta ggagcccgct tcggagcgga aacacggcgt
      901 gtgggctacc agttttcggg gaaggctgtc cgggtccagt atgctttcgc gcacgaggtt
      961 ggtcagcggc acctcggccg taggaatgag gtaaaaagga ggcttgcctt ccgtcctgaa
     1021 caggtcctgc tcgaatttcg ggagctgccc cgtacccgtc agcgcatccg cctgcacaag
     1081 ataagggacg taagtctctt catagccatg ttcccgcgtg tgcacgtcca gcatgaactg
     1141 gaccagcgcg cgctgcatat gtgcaagacc accgcgcaga acggtaaaac gcgaaccaga
     1201 catgcggctt gcaacctcga ggtccagcat cttcagtcgc gcgccaaggt cgacgtgatc
     1261 ccgcacctcg aaggaaaacg acgtcggctc tctgaagcga cgcacctcga cgttgccggc
     1321 ttcgccgcgg ccgaaaggga cgtcatctgc aagcaggttg ggtaaaccga gttcaacggc
     1381 ttccagttcc tgcaccacct cctgcagctc ccgctccgca gcttccagcc gctccccctt
     1441 gtccttcacc gacgcgagcc gtgcctggac atcctcgccc ctggcttttg cctgtcctat
     1501 ggccttcgca cttgtattct tttcgttgcg caacgattct acgcgcgtct gcagggcttt
     1561 gcgccgctcg tcgagccccc ggtaagccgc aacgtcgaat tcgatacctc gccgggaaag
     1621 attggcggca acctcctcgg tggattgccg caattgcttc gggtcaatca tctttgtttt
     1681 tttctttgcc tgtgcgttta ttgtcactga tacgttgcgt cagctcggcg ttccttgcca
     1741 gcttctcgcg gatctggagc tccaggccgc ggggcacggg atcataatac ctgcgcggcg
     1801 gcatgtcgtc cggaaagtag gtttctcccg cggcaaacgc atcctgttcg tcgtgcgcat
     1861 agcgatagtc cttgccatag cccaggtcct tcatcaggcg cgtcggcgcg ttacgcaggt
     1921 gcatcggtac ttccagcgat cccgattcgc gggcgtcggc cattgctgcg tttgccgcct
     1981 tgtacaccgc gttgcttttc gccgcacagg caaggaatac cacggcctgt gcgagtgcca
     2041 gttccccttc cgggcttccg agccgctcca gtgcatccca cgcgtcgagg gtgatacgga
     2101 gcgctcgagg atcggcattg ccgatgtcct cggaagcgac cctgaccatg cggcgggaga
     2161 cataaagagg atcgcaaccg ccgtcgagca tccgcatcag ccaatagagt gaggcgtccg
     2221 gatcggagcc acgaatggat ttatgcagcg cggagatctg gtcgtagaag tactcgcccc
     2281 tcttgtcgaa gcggcggcgc ccgccactcg cgacagcttc cgcgacagca aggtcaatcc
     2341 gctgctcgcc ttcggcctcc ggcgcgaggt ccgccgcaag ctccaccaaa ttcagcgcgc
     2401 gccgcgcatc gccgtccgcc gcggctacga tgagacgaag ggcatcctcg tcggctctta
     2461 taatgcgcga gtcgaacgcc tgcgccgacc gctcgagcgc gcgcctcaca atctgcatca
     2521 gcgagtccgc atcgagcggc ttcagcaagt acaccctcgc gcgcgacagc aacgcgttgt
     2581 tcagctcaaa ggaggggttc tcggtggtag cgccgataaa cgtcagcgtt ccgtcttcca
     2641 cgaacgggag gaacgcgtcc tgctgcgatt tgctgaaccg atgcacttca tcgaggaaca
     2701 ggatggtggc acggccgctc atctccctgt gttgccgggc ctcggccacc gcggcgcgta
     2761 tgtccttgac gccggccatg acggcggaca acccgatgaa atgagagtcc gtcgatgcgg
     2821 caatgatgcg cgcgagcgtg gtcttgccgg tgccgggagg gccccagaag atcatcgagt
     2881 gggcgcggcc ttgctcaatc gccatgcgca ggggcttgcc cccgtccaac aagtggcgct
     2941 gaccgaagaa atcatcgagg gaggcaggcc gcaggcgatc cgcaagcggc ctgcccgcct
     3001 gcttgcgctg gtcgccgctt gaaaagagat cgttcacttc aggcacctcg ccacgcaggg
     3061 tttatcgccc cggccgaatc cccggtcagt ctctcgcagc gatccatcgt tcgaccttga
     3121 ggctccagcc gccgacgccg gcccatgcaa tcagaagccc ggccaggatc cccaccgcat
     3181 ccgcgaccag gtccaggatt tcggcactgc gataggtagt cagcatgcgc tggcagagtt
     3241 caatgagcgc gccgaacacg agcataccgg cggcgatgcg ccagtagctg cgtcttgcat
     3301 actgcccgct gaaccagatc gtcagcagca tgaacgtgat cgcgtggaac cacttgtccg
     3361 aatgcgcaat gcgcggatcg cgcatttcct gcatgaacca gatcgcgggc aacagtgtcg
     3421 ccgccaggac gatggcaagg actgcgattc cggcaagctg ccagcgccgg gcatggcgaa
     3481 gtggaagcat ccaggccact agtcttttaa cggcgtgccg accacgtcga ctccctccgg
     3541 tggaacgaaa cggaaacgtt ccggatctat ggactcgttc aatacgacgt cgtgcatcgc
     3601 gacgagtgta gtctgatcaa ggttgtcggc aaagatcata cgggtaagcg tgccatcgct
     3661 gaatccgagt tcgacgctct cgaagttgct gtccgggtcg cgcggcagca gtcgtaccca
     3721 ttccgtgccg cgatcctcga agctgccgtc gtaagtgaaa tcctcgagga cgtcatgtgc
     3781 cccgccgagc agcagggctg gggtgctact cagcacttcg gcctgcggtt tgaccgttac
     3841 ctgtgcgaga tcgacgtcgt aactccagac attcagcccg tccgcgacga ggagttgctc
     3901 ataaggctgt tcataggacc agcgtaactg tccgggacgc ctgatctcga aggtgccgct
     3961 ggacgattcg acgatggtgt cattggcgtc gaccagcgac tgctcgaaac gaccacgcat
     4021 ggtgcgcaca ctctccacga agttctcgat gagctgttcg ccattgactt ccggcggctc
     4081 ggcagcaccg gcgcttgcgc ctgccaagaa aaaagacgcg gcaacaagcc ggccgagggt
     4141 ggtgttcatg acgggcatag ggtcagggtc tcttttggta tagcgtgcaa cggtagacgc
     4201 caggcttcgg gcgcggcctt ccggcaaacg tgttggaccg gcttc
//
LOCUS       DASUOM010000253         5196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1275544,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000253 DASUOM010000000
VERSION     DASUOM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275544"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2668)
                     /locus_tag="VFY27_05245"
     CDS             complement(<1..2668)
                     /locus_tag="VFY27_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5764953.1"
                     /translation="MKLLRTLLTNHPLVNIFFAVVIVMGLVSYLQLPREQDPEVNFNF
                     VNINTVLPGATAADVEELVTGPLEDALRNVQDIRFVSSSSRENVSDILVRFNELSERE
                     FDKRVVDLRREIQSKTNDELPDDVEDPYVLEVTTSNGFPTATVIVAGQADDERLRRQA
                     KIIRDELERLTGVDRVQAFGFNEPELQVEVDPQALAANGLTATDVADQLREAYRDVTA
                     GEMEVGSEAWLIRVEGKTVLPEELARFLVSPRNQPQRKVSLDRIAEIRRGREEEQQLV
                     SFNGRPAVSMSITKVAFTNTLELVDGINHYVDERNRQTEGTGIDLYLADDQTTQTREA
                     LSVMQTNALLGLFLVLAVCWVFLGLRIAFFVTLGIAFSIAGTFWILNITGNTLNVPVL
                     LGIVIVLGMLVDDAVVIVEAMYYRLQRGEEALQAALDSLREVGQPVTAAVFTTISAFA
                     PLMLLPGILGDFMKIIPLVVTTGLLVSLIEAFWILPSHVITSTARVQPASDRFSHWRG
                     RWTHLVRVRYTRALAYVFRRPKRFFLAGLLAFALSIVAVATEQVRVEFFTFDPFRLFY
                     VHVELPPEAPLEETLAQAERVEQRIRTLIEDDEIRTITVMAGVKFTDAEALFGDQYGQ
                     IQVSLMPKTPGNRAVGDIVDAIRDVALATPGNGEISFFEVSGGPPVAKDVSVKVRGDD
                     FEELRAAAEEVKRIVSSIEGSANVIDNDVPGRFELALLPDVRAIRQAGLAPGEVLRLL
                     RLHLDGEIVAFTRDGGEKLELRVRGPRRTVEDASQILADPVALPGGGTTTLGALSSSN
                     IERGSGTIRHYNFRRSITVEADLDEEVINQVAATNRIIEEWEKVRQQYPNIALDFSGA
                     FDDIQESLDSLLILGLFGVGLIYLILATQFRSY"
     gene            complement(2665..3633)
                     /locus_tag="VFY27_05250"
     CDS             complement(2665..3633)
                     /locus_tag="VFY27_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5764954.1"
                     /translation="MIPHVAFAEIPVTVQDLGETLVAREYRAPATVITANRTLVTSQV
                     TALIEDITVDVGDEVKQEQLLVKLDPADARLALAEAEAALAALNAQLLDARQRLQRAE
                     DLLERSFVSEDELNIRRTEVSVIEANRERQLVAIQDARLALARTEVRAPFEAAVVERQ
                     GQVGSLASPGSPLLTLVQTAGREVDAEVDPRHASQLKEASDLRFVSNGQEWPLELARL
                     SPVIETDTRKVRGRFDFSAAVAPIGATGEVVWREPSSLVPVSLIVQRGNELGIFMASD
                     GRARFIAIPTAQEGRPAEVDLPVDTPIVTKGHVRLQDGDAVQITRE"
     gene            3837..4448
                     /locus_tag="VFY27_05255"
     CDS             3837..4448
                     /locus_tag="VFY27_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764955.1"
                     /translation="MSGILMPFGKFAFYLFACLLCACAAPEVLVPKSGAAPASVDFSG
                     TWRLRRDESDGQRRINEAIRRTDGVNDRIILRPPDPRDERSSSARSRRNPGGLVHVFL
                     ENGNTLKITQTADGMFLSFDRAVVVEYRFGENRQVQVGPVVAARVSGWEGREYVAETL
                     DEDRMKLTERFALLNRHTLQRTIIFRGKNNEEETLVQTFDLVG"
     gene            complement(4586..>5196)
                     /locus_tag="VFY27_05260"
     CDS             complement(4586..>5196)
                     /locus_tag="VFY27_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5764956.1"
                     /translation="VRISVLSGTLVSEVTQTGPGYMANTVIKPTGVSRMLARGVIQES
                     SYFLPDSDGVLPAQYRSIDTLSSDDAVVSLDFDWREQQVEGVVNEKKFETTLDDRVHD
                     RVSIQYELMHDLRNGGAEDQYWMLDGDELKLLNVNNVGTRKVKVPYGEFEVVGIQHSK
                     QNSSRITTLWCAEELGYLPVIIEQHRKGKLGVRAVLRTYEPT"
BASE COUNT          984 a         1597 c         1581 g         1034 t
ORIGIN      
        1 aatagctgcg gaactgcgtg gccaggatga gataaatcaa tcccacgccg aacagcccga
       61 gaatcagcag tgaatcgagg ctttcctgga tgtcgtcgaa cgctccggaa aaatccaggg
      121 cgatgttcgg gtattgctgc cgcactttct cccattcttc gatgatccgg ttggtggcgg
      181 caacctggtt gatgacctct tcgtccaggt cggcttccac ggttatcgac cgccggaagt
      241 tgtagtgccg gatggtgccg ctcccgcgct ctatgttcga actggacagt gcaccgagcg
      301 tggtcgtgcc gccgcccgga agtgccaccg gatccgccag gatctggctt gcatcctcga
      361 ccgtgcggcg cggaccgcgc actcgcagct ccaatttctc gcccccgtcg cgggtgaaag
      421 cgacgatttc gccgtccagg tgcagtcgca agaggcgcag cacttcgccg ggtgcgaggc
      481 ccgcctggcg gatcgcccgt acgtcgggca acagcgccag ctcgaagcgg cccggaacgt
      541 cgttgtcgat gacattagcg gagccttcaa tgctgctgac gatgcgcttc acttcttcag
      601 ccgccgcccg cagctcctcg aagtcatccc cacgcacctt gacgctgacg tccttcgcga
      661 ccggaggacc tccggatacc tcgaagaacg aaatttcgcc attcccgggc gtcgccagcg
      721 caacgtcgcg gatcgcatcc acgatgtcgc cgacagcgcg gttgccgggt gtcttcggca
      781 tcagcgaaac ctggatctgg ccgtactggt ccccgaagag cgcctcggcg tcggtgaatt
      841 tcacgccggc catcaccgtg attgtccgga tctcgtcgtc ctctatcaga gtgcgaatcc
      901 tctgttccac ccgttccgcc tgtgccagcg tctcttcgag cggcgcttcc ggcggtaatt
      961 cgacgtggac gtagaacagg cggaacggat cgaacgtgaa gaactccacg cgcacctgtt
     1021 ccgtcgcgac ggcaactatg gaaagggcga atgcgagcag gcccgcgaga aagaagcgct
     1081 tcggccgtcg gaagacgtac gcgagtgcac gcgtataccg aacccgcacc aggtgcgtcc
     1141 agcggccccg ccagtggctg aaccggtcgc ttgcaggttg tacgcgcgcg gtggacgtga
     1201 tgacgtgaga aggcaggatc cagaacgcct cgatcagact gacgagcagg ccggtcgtaa
     1261 ctacgagggg aatgatcttc atgaagtcgc cgaggatgcc cggcaacagc atcaggggcg
     1321 cgaaggccga gatcgtcgta aacacggcgg cggttaccgg ttgcccgact tcccgcagtg
     1381 agtcgagcgc agcctgcaaa gcttcctcgc cacgctgcag ccggtagtac attgcctcca
     1441 cgatcacgac agcatcgtcc accagcatcc caaggacgat gacgatgccg agcagaaccg
     1501 gaacattcag cgtattgccc gtgatattca gtatccagaa ggtaccggca atggagaacg
     1561 caatacccag tgtcacgaaa aacgcaatgc gcaggccgag gaacacccag cagaccgcga
     1621 gcaccaggaa cagcccgagg agggcgttcg tttgcatgac cgacaacgcc tcgcgcgtct
     1681 gtgtggtctg gtcatcggcg aggtaaaggt cgattccagt tccttccgtc tgccgattgc
     1741 gttcgtcgac gtaatggttg ataccgtcca cgagctccag cgtattcgtg aacgcaactt
     1801 tggtgatgga catggaaacg gcagggcgac cgttgaagct gaccagctgt tgctcctctt
     1861 cccggccgcg acggatctcg gcgatgcgat ccagggaaac ctttctctgc ggctggttcc
     1921 tcggcgacac caggaatcgg gcgagttcct ccggcagtac ggtctttcct tcgacacgaa
     1981 tgagccaggc ttcgctgccg acttccatct caccggccgt cacgtcgcga tacgcctccc
     2041 tgagctggtc ggcaacatcg gtcgccgtca acccgtttgc cgccagtgcc tgcgggtcga
     2101 cctccacctg caattccggt tcattgaagc cgaacgcctg tacgcgatcg acgccggtga
     2161 ggcgctcaag ttcgtcccgg atgatctttg cctgccgtcg caaccgttcg tcatccgcct
     2221 ggcccgcaac gatgactgtg gcggtaggaa agccgttcga tgttgttacc tcgagaacgt
     2281 acggatcttc gacgtcgtcc ggtagctcgt cattggtctt gctctggatc tcccggcgca
     2341 ggtcgacgac acgtttgtcg aactctcgct ccgaaagctc attgaaccgc acgaggatgt
     2401 cggagacgtt ttcgcgggag gaactgctga cgaagcgaat atcctgcacg tttcgcaagg
     2461 cgtcctctag cggccccgtg acgagttcct cgacgtcggc agcggtcgcg cctggcagca
     2521 ccgtgttgat gttgacgaag ttgaaattga cttccgggtc ctgctcgcgc gggagctgca
     2581 ggtatgagac aaggcccatc acgatcacca ccgcgaaaaa aatgttgacc agcgggtggt
     2641 tggtcaacag tgtccggagc agcttcattc ccgcgtaatc tgtacggcgt ctccatcctg
     2701 caggcgcacg tggcctttcg tgacgatggg cgtgtcgacc ggcaggtcga cttccgcggg
     2761 acgtccttcc tgtgccgtcg gtattgcgat gaaacgggcg cgcccgtcgc tggccatgaa
     2821 gatgccgagt tcattgccac gctggacgat caacgaaaca ggaaccaggg aagacggttc
     2881 gcgccacacg acttcgccgg tggcacctat cggtgcgacc gctgcggaga aatcgaatcg
     2941 gccgcgcact ttgcgggtat cggtttcgat taccggcgaa agccgcgcga gctcgagtgg
     3001 ccattcctgc ccgttgctca cgaagcgcag gtcggatgct tccttcaact ggctggcgtg
     3061 tcgagggtcc acttcggcat cgacttcccg ccctgccgtc tgtaccagcg tgagcagggg
     3121 acttccaggc gacgcgaggc tgccaacctg gccctggcgc tctacgactg cagcctcgaa
     3181 cggggcacgt acttcggttc gtgcgagcgc caggcgcgca tcctgtatgg cgacaagctg
     3241 ccgctcgcga ttcgcttcga tgaccgagac ctcggtgcga cggatgttca gctcgtcttc
     3301 cgagacgaag gatcgctcca gaagatcctc cgcgcgctga agtctctgcc gggcatccag
     3361 gagttgcgca ttgagcgccg ccagtgctgc ttcggcctct gcaagcgcga ggcgtgcgtc
     3421 agccgggtcc agcttcacaa gaagctgctc ttgctttacc tcatccccga catcgacagt
     3481 aatatcttcg atcagagccg ttacttgcga cgtgaccagc gtgcgatttg cggtgatgac
     3541 ggtagcgggc gcacggtatt cgcgcgcgac cagcgtctcg ccaaggtcct gcaccgttac
     3601 cgggatctcc gcaaatgcga cgtgcggaat catcgcggcg acggctagga gtgccgttgt
     3661 ttttcctgaa gccatttcgt cgagccaatg tgaccttttg gacaatccag caagagcgtc
     3721 ccgcaactgc gggagattga cctcgaaagg ggaacttcgg tctaatgggg tgaagctggc
     3781 gggcgattat aaggtgctgg tccgaccgat gcagcatccc tcaaattgat ggtggaatgt
     3841 ccggaatatt gatgcccttc ggcaagtttg ccttttacct gttcgcctgt ctgctctgtg
     3901 cctgcgcggc acccgaggta ctggtaccca aaagcggtgc tgcgccggcc agcgtggatt
     3961 tctcgggcac ctggcggcta cgtcgggacg agagtgacgg gcagcggcga atcaacgaag
     4021 caatccggcg caccgacggt gtgaacgacc ggataatcct gcgccctccg gatccccggg
     4081 acgagcgcag cagctcggcg cggtcacggc gcaaccctgg cgggctcgtt catgtcttcc
     4141 tggagaacgg taacacactc aaaataaccc agacggcgga cggtatgttt ttaagcttcg
     4201 atcgcgcggt ggttgtggag tatcgcttcg gcgagaatcg gcaggtgcag gtaggacccg
     4261 tagtcgcggc ccgtgtctcg ggctgggaag ggcgggagta cgtcgccgag acgctggatg
     4321 aagatcggat gaagctgacg gagcgattcg ccctgctcaa caggcatacg cttcagcgca
     4381 cgatcatctt tcgcggcaag aacaatgaag aggaaaccct ggtgcagacg ttcgacctgg
     4441 tgggctgagc cggggagtct ctgcacgtag cagcctgccg tgcgggcgag tgcgatgtgc
     4501 cgtaccgaac tcgctcgcat ggcgagctcc tacatcgcct tgaatttgct tcgcaaacct
     4561 gtcagtcctg cgcagccacc gactctcacg tcggctcata ggtcctcaga accgcgcgaa
     4621 ccccaagctt gccttttcga tgctgctcga tgatgaccgg caggtaaccg agttcttctg
     4681 cgcaccagag cgtcgtgatg cggctcgagt tttgcttgct gtgctggatg ccgactacct
     4741 cgaactcgcc gtacggcact ttcaccttcc gcgtgccgac gttattcacg ttcagcagct
     4801 tcagctcgtc gccgtcgagc atccagtact ggtcttcggc gccgccgttt cgcaggtcat
     4861 gcatcagctc atactggatc gagacgcgat cgtgcacgcg gtcgtcgagg gtagtctcga
     4921 acttcttctc gttgactacg ccctcgactt gctgctcgcg ccagtcgaag tccagggaaa
     4981 cgactgcatc atccgatgag agcgtatcga tggaacgata ctgcgcaggc aaaacaccgt
     5041 cgctgtccgg aagaaagtag gagctctcct ggatgacgcc gcgcgcgagc atgcgcgaga
     5101 cgccggtcgg cttgatcacc gtgttggcca tgtagccggg ccctgtctgc gtcacctcgg
     5161 acaccagagt cccgctgagc actgatatcc tgacgt
//
LOCUS       DASUOM010000254         9949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1058149,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000254 DASUOM010000000
VERSION     DASUOM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058149"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1329
                     /locus_tag="VFY27_05265"
     CDS             121..1329
                     /locus_tag="VFY27_05265"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5764957.1"
                     /translation="MTDAYIYDHVRSPRGRGKPNGSLHEVTPVDLASQVLTSLRDRNQ
                     LDTGRVDDVVFGCATPVGEQGACIARTAVLNANFDQSVPGKQLNRFCASGLEAVNTAA
                     AQIMAGQSDLTIGGGVECMSRVAMGSDSGAMHADPAVAYKIYFAPQGIGADLIASKHG
                     FTREDVDAYAVESQKRAANAWEQGYFSKSVTPIRDLIGQVLLDRDEHLRPGTTMDDLA
                     GLKPAFAVIGEQAGFDAVALQKYPDVEFIDHVHTGGNSSGIVDGAAAVLLGSKDAGDA
                     LGLKPRARIRGFSSIGTDPTIMLTGPAPSAEKVLKRCGMTVKDIDLFELNEAFASVVL
                     LYMRRLGIEHDRINVNGGAIALGHPLGATGAMILGTVLDELERRDLNTALVNLCVGGG
                     MGTATIIERV"
     gene            1583..3766
                     /locus_tag="VFY27_05270"
     CDS             1583..3766
                     /locus_tag="VFY27_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5764958.1"
                     /translation="METIEFDTGADGIVVLTIDVKDRSMNVMTPGFLADLAGAVDTIA
                     TDDGIKGAVITSGKESFMAGADLVGLVESFEQRTNAREVYAECRGLQQTLRRLETCGK
                     PVAAAINGTALGGGLEICLACHYRVAAERPGAVLGLPEVQVGLMPGGGGTQRLSRLIG
                     IEPALRLITEGRHVAPAEALTLGIVNAVAPVGQELDAAREWVRNGGVSEQPWDVKGFR
                     FPGGAGLMHPAAVQTQMVGTALLQKQTNHNYPAPIAIMSAVYEGSVLPMDKALDVETK
                     YFVSLLMSPVSRNMMRTLFINKGAAEKLVRRPKGPRKREVKKLGVLGAGMMGAGIAYV
                     SAVAGIEVVLLDTTAEKANAGKEHSRALLQQRVEKGKLGNEEAAAVLARIDATESYDR
                     LQGCELVIEAVFEDREIKADVTNRAESVIPEDAVFASNTSTLPISGLAEASRRPAQFI
                     GIHFFSPVEKMPLVEVIVGDATGDVALAQALDYVQQLRKTPIVVNDSRGFYTSRVFSV
                     YSREGMTMLAEGIDPALIENVAKHAGMPVGPLAVTDEVSLELSYHILQQTKADLGEAY
                     VATPADRVLRLFVEELGRKGKRSGAGFYEYGETSGPRGKRLWPGLKEHFPPAAVQPDS
                     GEVRKRLLYAQAIETARCLDENVVTHPADADIGSVFGWGFPPYTGGTISFIETEGLKA
                     FVAEADRLAEKHGNRFKVPESLRTMAANGETFYGRVSAAKHGNAA"
     gene            3776..4558
                     /locus_tag="VFY27_05275"
     CDS             3776..4558
                     /locus_tag="VFY27_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5764959.1"
                     /translation="MSELDTVRYKQEEAVAVITIHRPEAMNSFNTGLRRDLLAAFRQA
                     RGGRTVRVVVLTGEGRSFSAGADLKGSLSSDRSVEEQLQQEYRPIFECIAEMDQPVIA
                     AVGGSAAGIGMSYALACDLLVMAEDAFLLSPFTTISLVPDGGLNWLLVHQLGYRRAFQ
                     LSIECERIPAERCVQLGLANRTAPAESLLDDTIAWARSIAERAPLSLAATKKAMRHAA
                     GHDWASTFDVEAPLQRKLRESEDCAEGVAAFFQKRKPEFKGR"
     gene            4767..5951
                     /locus_tag="VFY27_05280"
     CDS             4767..5951
                     /locus_tag="VFY27_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5764960.1"
                     /translation="MTRHIFEEEHDMFRDSVRAFMKKEILPNTDRWHEQGIVDREAFL
                     KAGEQGLLLMWADEKYGGAGVQDFRYEQVLIEENARYGDAGFFMTLHSRLVGPYIGEL
                     GTEEQKARWLPKCISGEHILAVAITEPGAGSDVAGIRTRADDKGDHYLLNGSKTYISN
                     GINADLVVVVAKTDPGARHGLSLFVVERGMHGFERGRNLKKMGLKSQDTAELFFNNVK
                     VPKANLLGELHKGFYHLMHFLAEERLLGAVGYLAAADAAFDITMDYIRERKAFGQLIA
                     EFQNTRFKMADMRTEIDVAQAFVDQCVVEHNRHELTADNAAKAKLFTSELEGRVMDQC
                     VQLHGGAGYMDEYPISRMYLNARVSRIYAGSSEVMREIIARSLGLDPRKQQSSLTSRS
                     AA"
     gene            5951..6820
                     /locus_tag="VFY27_05285"
     CDS             5951..6820
                     /locus_tag="VFY27_05285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5764961.1"
                     /translation="MKVAFVGLGVMGYPMAGHLAASGHDVTVYNRTASKADAWCREHE
                     GRKAASPARAAEDAEIIFYCVGNDDDVRAVVLGADGILSGVRQGGGIVVDHTTASAVV
                     AREIHAAAADKGAGFLDAPLSGGQAGAENGQLTIMTGGEREVFERALPVMQSYAKACT
                     LIGPVGSGQLAKMVNQICIAGIVQGLSEALHFAKRAGLDVEAVIGAISRGAAQSWQME
                     NRWKTMVNDQFEFGFAVEWMRKDLKIALDEAARNGATLEMTALVDKYYKEVEEMGGAR
                     WDTSSLIRRLEKK"
     regulatory      6996..7123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7119..9236
                     /locus_tag="VFY27_05290"
     CDS             7119..9236
                     /locus_tag="VFY27_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5764962.1"
                     /translation="MFHSISRQGSVFAYRAMILLFAAVASATADEVLEEIIVTADFRE
                     RPADEMAASITVLDTDSIEQLAVQHFEEMVFFVPNLNWSGDGNRARYFQIRGVGELEQ
                     YEGAPNPSVGFIIDDIDFSGIGTIATLFDIEQIEVLRGPQGSRYGAQALAGLIYVQST
                     EPDDEWSGRVQVGGGQDNAMSGGVALGGPLTTDGALRFRLSGHHHESDGFRENSYLGS
                     SDTNGRKESTARAKMAWDASDDWQLKLATMWSDIDDGYDGFVVDNSLTMLSDKPGRDA
                     QRSLGAAFTAGWSGSDRFDFTSITSAAKSDIDFSFDADWGNPESWAPFTYDFISANDR
                     ERRTLSQEFRVAAGPEGRLFNGSTDWLIGAYYLKLEDELATLNQGEYFDPLAGFADRL
                     DDRFSSSFDASNVALFGQMDIDVGESGEVGIGLRVENRSTDYFDSAGLQLDPGETMLG
                     GELSYRHSFDDGLSAYVSLSRGYKAGGFNLGVVPEGRREFGQEAMWNIESGLKALWLD
                     DTLLFNGAIFYSRRDDQQVRTSVQLAPNDPASFVFFTDNAAEGRISGLEAELRWLPTE
                     SLEFYTNVGLLDAEFSEFADAEGRLSSRDQAHAPNFTLALGGMYRRANGFFARLDLSA
                     RDEFYFDVSHDQKSERHALANARVGFDTERWTAQVWMRNAFDENYAVRGFYFGNEPPD
                     FPTKLYVRRGDPRQLGFTVDMRF"
     gene            9236..9490
                     /locus_tag="VFY27_05295"
     CDS             9236..9490
                     /locus_tag="VFY27_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEX5764963.1"
                     /translation="MMRIAVDISLYPLSSDFLPPIKDVISRLNSYPSLEVETNPMSTQ
                     IRGEYDEVMNALQQEMKQTFEDVPKAVFAIRILNNPMSTP"
     gene            9574..>9949
                     /locus_tag="VFY27_05300"
     CDS             9574..>9949
                     /locus_tag="VFY27_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HEX5764964.1"
                     /translation="MTGITAQLQALSPAEVIAVAAAVAYLFFAIRQNILCWFFALVST
                     AIYIGLFFGAKLYMESVLNIFYFGMALYGWYFWLAGGGRESTLPVTRWPLLPHVLAVG
                     GILLLSLLFGSLLDRYTQAAYPY"
BASE COUNT         2001 a         2920 c         3230 g         1798 t
ORIGIN      
        1 cccggagaac aagcggcttt gcgcaacggc cacaggtacg tacaagcggc aggcgcgggc
       61 cccgctcaag tcgcgcaagt ccgcaaggta tgactctgat gcagcaggga aggccaggac
      121 gtgacggacg cttatatcta cgatcatgtg agaagcccgc gcgggcgcgg caagcctaac
      181 ggttcgcttc acgaagtgac gccggtcgac cttgcgtcac aggtactaac gagcctgcgc
      241 gaccgcaacc agcttgacac cggtcgcgtg gacgatgtcg ttttcggttg tgcgacgccg
      301 gtgggcgagc agggcgcctg catcgcacga acggcggtgc tcaacgcaaa tttcgaccag
      361 tcagtgccgg ggaaacagtt gaaccgcttc tgtgcttcgg gactggaagc cgtgaacacc
      421 gcggcagcgc agatcatggc gggacagtcg gacctcacga tcggcggggg cgtcgaatgc
      481 atgtcgcgcg tcgcaatggg ttccgattcc ggtgcgatgc acgcagaccc cgcggtcgcc
      541 tacaagattt acttcgcgcc gcagggaata ggcgccgacc tcatcgcgag caagcacggt
      601 tttacgcggg aggacgtcga tgcctatgcg gtcgaaagcc agaagcgcgc cgccaacgcg
      661 tgggagcagg gctacttttc caagtcggtc accccgatcc gcgacctgat tggccaggta
      721 ctcctggacc gcgacgagca cctgcgcccc ggcaccacta tggacgacct cgcgggactg
      781 aaacccgctt tcgctgttat cggcgaacaa gccggcttcg acgcagttgc cctgcagaag
      841 tatcccgacg tcgaattcat cgaccatgtg cacacgggcg gtaattccag cggtatcgtc
      901 gacggtgcgg ccgccgtact tctcggcagc aaggacgccg gcgacgcatt gggcctgaag
      961 ccacgcgccc gcatccgcgg gttctcctcc atcggcaccg atcccacgat catgctcacc
     1021 ggccccgcgc cgtctgccga gaaagtgctg aagcgctgcg gcatgacggt gaaggacatc
     1081 gacctgttcg aactgaacga ggcattcgcc tcggtcgtcc tgctctacat gcggcgcctg
     1141 ggcatcgagc acgacaggat caacgtcaat ggcggcgcca tcgccctggg ccatccgctg
     1201 ggcgccaccg gcgcaatgat tctcggcacc gtactcgacg aactggagcg gcgcgatctc
     1261 aacaccgccc tcgtgaacct ttgcgttggc ggcggcatgg gtactgctac gatcattgag
     1321 cgggtgtgac gggcggcggc gttcagggtg tgaccggcct gcagtgttca tgaacggtaa
     1381 gggacggtgg tgggcggagc ctcggctggg gggagggctt gtcaggaacg ccgtgaatcc
     1441 atccatggag gctttgcgca aacatccctg tttgcgacaa tcctgacaag ccctcccccc
     1501 aaccaagact ccgccaagat gcggtgtgcc ggttcatgaa cactgcgggc cggtcacacc
     1561 ctggatcgcg atagtgaggc ttatggaaac cattgagttc gacacaggcg ctgacgggat
     1621 tgtcgttctg acgatcgatg tgaaggatcg gtcgatgaac gtgatgacgc ccggcttcct
     1681 cgcggacctt gccggtgccg tggacacgat cgccaccgac gacggaatca aaggcgcggt
     1741 tatcacctcg ggcaaagagt cattcatggc cggcgcggat ctggtgggtc tcgtggagtc
     1801 tttcgagcag cggaccaacg cccgggaagt ctatgcggag tgccgcgggc tgcagcagac
     1861 gctgcgccgg ctggagactt gcggcaagcc cgtcgcggct gcgatcaacg gcactgcact
     1921 gggcggtggt ctcgaaatct gccttgcgtg ccactaccgg gttgcggcgg agcggccagg
     1981 ggcagtcctg ggcctgccgg aggtgcaggt ggggctgatg cccggcggcg ggggtacgca
     2041 gcggctgagc cggctgatcg gtatcgagcc tgcgctgcgc ctgatcaccg aaggccggca
     2101 cgtcgcgcct gccgaggcgc tgacgctcgg catcgtgaac gcggtggcgc ctgtcggcca
     2161 ggagctcgat gccgcgcgcg agtgggtgag gaatggcggt gtcagcgaac agccatggga
     2221 cgtgaagggc tttcgttttc ccggcggtgc gggtctcatg catcccgccg cggtgcagac
     2281 gcaaatggtc ggcacggcgt tgctgcaaaa gcagacgaac cacaactatc ccgcgcccat
     2341 cgcgatcatg tcggcagtgt atgaaggcag cgtgttgccg atggacaagg cgctggacgt
     2401 cgagacgaag tatttcgtat cactgctcat gagtcctgtg tcgcgcaaca tgatgcgcac
     2461 gctgttcatc aacaagggtg ccgcggagaa gctcgttcgc cgcccgaagg ggccgcggaa
     2521 gcgcgaggtg aagaagctcg gggtgcttgg cgcgggcatg atgggagcgg gaatagcata
     2581 cgtttcggcg gtggcgggca tcgaagtcgt cctgctggat accacggcgg agaaggcgaa
     2641 cgcaggcaag gaacactcgc gtgcgctgct gcaacaacgt gtcgagaagg gcaagctcgg
     2701 aaacgaagag gcagcagccg tactggcgcg catagacgcg acggagagct atgacaggtt
     2761 acaaggctgt gaactggtca tcgaggccgt tttcgaagac agggaaatca aggccgatgt
     2821 caccaacagg gcagaatcgg taattccgga agacgccgtt tttgcctcca acacgtcgac
     2881 actgccgatc agcggacttg cggaggcttc gcggcgtccc gcgcagttca tcggcatcca
     2941 tttcttctcg ccggtggaga aaatgcccct ggtcgaagtt atcgtcggtg atgccacggg
     3001 tgatgtcgcg ttggcgcagg ctctcgatta cgtgcagcaa ctgcgcaaga cgccaatcgt
     3061 cgtcaacgac agccgcggtt tctacaccag ccgggtattc agcgtataca gccgcgaagg
     3121 catgacgatg ctcgccgaag gcattgatcc tgcactgatc gaaaacgttg cgaagcatgc
     3181 gggaatgccc gtcgggccgc tggccgtcac cgacgaagtt tcgctggagc tgagctacca
     3241 catcctgcag cagacgaagg ccgacctggg cgaggcctac gtagcgacgc cggcggaccg
     3301 tgtgctacgg ctgttcgtcg aggagctcgg gcgcaagggc aagcggtcgg gcgccgggtt
     3361 ctacgagtat ggcgaaacca gcgggccgcg tggcaagcgg ctgtggccag ggcttaagga
     3421 gcacttcccg ccggcggccg tgcagcccga cagcggcgag gtgcgcaaac gcctgttgta
     3481 cgcgcaggcg atcgagacgg cgcgttgcct ggacgagaac gtggtgacac atcccgcgga
     3541 cgcggatatc gggtcggtct tcggctgggg cttcccgccc tataccggcg gaacgatttc
     3601 cttcatcgag acagaaggcc tgaaggcctt cgttgccgag gccgaccgtc ttgccgaaaa
     3661 gcacggcaat cgcttcaagg tccccgaaag cctgcgcacg atggcagcga acggcgagac
     3721 tttttatggc agggtttcgg ccgccaaaca tggaaatgcg gcctgaggat tggtgatgag
     3781 cgaactcgac acagtgcgtt acaagcagga agaagctgtt gccgtgatta ccattcatcg
     3841 cccggaagcg atgaactcgt tcaacaccgg attgcgacgc gatctcctcg ctgcattccg
     3901 ccaggcaaga ggcggccgga ccgttcgcgt ggtcgtgctc accggcgaag ggcggtcgtt
     3961 cagtgccggc gcggacctca agggaagcct gtcgagcgac cggagcgttg aggaacagct
     4021 gcagcaggag taccggccta ttttcgagtg catcgcggag atggaccagc ccgtcattgc
     4081 cgcggtggga ggttcggcgg cgggcatagg catgtcctac gcgctggcct gcgatttgct
     4141 ggtcatggcc gaggatgcgt tcctgctgtc cccgttcacc acgatatcgc tggttccgga
     4201 cggcggcctt aactggctgc tggtacacca gctcggttac aggcgcgcct tccagctcag
     4261 catagagtgc gaacgcatac cggcggagcg gtgcgtgcag ctcggcctgg cgaacaggac
     4321 cgcccctgcg gaatccctgc tcgacgacac tatcgcctgg gcgcgctcta tcgcggagcg
     4381 ggcgccgctg tctctcgctg caacgaaaaa agccatgcgt catgctgccg gtcacgactg
     4441 ggcaagtact ttcgacgtcg aagctcctct gcagcgcaag ttgcgggaga gcgaggactg
     4501 cgccgagggc gttgcggcgt tcttccagaa gcgcaagccc gagttcaagg gccgctaacg
     4561 atgtaggata ccacgccgaa cggaaccggc aacgatctca atcaggtgcc ttgcgggcga
     4621 tgaatgcggg cgagtgcgat cattcgtcag cgaactcgcc cgcatggcgg gctcctacgg
     4681 tgccaacagg gagcttgcca tcggttagcg agtacgaggc ggcgaataaa tcaggtaaac
     4741 gaatcgaaat atcaggacag ctaatcatga cccgacacat attcgaggaa gagcacgaca
     4801 tgtttcgtga ttctgttcgt gcgttcatga aaaaggaaat cctgccgaac acggaccgct
     4861 ggcacgagca gggaatcgta gatcgcgaag cattcctcaa agccggcgaa cagggtctgc
     4921 tcctgatgtg ggccgatgag aaatacggcg gcgcaggtgt ccaggatttt cgctacgagc
     4981 aggtcctgat cgaggagaac gcccgctatg gcgacgcggg atttttcatg accctgcata
     5041 gccggctggt gggtccttat atcggggaac tgggcaccga ggaacaaaag gcccggtggt
     5101 tgccgaagtg catcagcggg gagcacatcc tggcagtggc tatcaccgaa cccggtgcgg
     5161 gcagcgacgt ggccgggatt cgtacgcgcg cggacgacaa gggcgaccat tacctgctga
     5221 acggctccaa gacttatatc tcgaacggca tcaacgccga cctggtcgtc gttgtcgcaa
     5281 agacggaccc gggcgcgcgc cacgggctca gcctgttcgt cgttgagcgc ggcatgcacg
     5341 gattcgaacg cggccgcaat ctcaagaaga tgggcttgaa aagccaggac acggcagagc
     5401 ttttcttcaa caatgtgaaa gtgccgaaag cgaacctcct gggcgagctg cacaagggtt
     5461 tctatcacct gatgcacttc ctggcggaag agcggctgct cggagcggtt ggctaccttg
     5521 cggctgcgga cgctgcattc gacatcacca tggattacat cagggaacgc aaggcgttcg
     5581 gccagctcat cgcggagttc cagaacacgc gcttcaagat ggcggatatg cgaaccgaga
     5641 tcgacgtggc gcaggcattc gtggaccagt gcgtggtgga gcataaccgg cacgaactga
     5701 ctgccgataa cgcagccaag gcaaagctat tcacgagcga gctggaaggg cgcgtgatgg
     5761 accagtgcgt ccagttgcac ggcggcgcag gctacatgga cgaatacccg atcagccgca
     5821 tgtacctgaa tgccagggtg tcgcggattt acgccggaag ctccgaggtc atgcgggaaa
     5881 tcatcgcgcg gtctctcggg ttggacccgc gcaagcagca gtcatcgcta acttcgcgga
     5941 gtgcggcata gtgaaggttg cgttcgtcgg cctcggcgtc atgggttacc cgatggctgg
     6001 ccacctcgcc gcgtcggggc acgatgtcac cgtttacaac cgaacggcct ccaaagccga
     6061 cgcatggtgc agggagcacg aaggtcgaaa agccgcctcg ccggccaggg ctgcggagga
     6121 cgcggaaatc atcttttact gcgtcggcaa tgacgatgac gtgcgggcgg ttgtgctcgg
     6181 ggcagatggc attttgagcg gtgttcggca aggaggaggg atcgtagtcg accacacgac
     6241 cgcctcggcg gtcgtggcgc gtgagattca cgcggccgct gcagacaaag gcgcaggctt
     6301 cctcgatgcg ccgctgtcag gcggacaggc gggcgcagaa aacggccagc tcacgatcat
     6361 gaccggcggc gagcgggagg tattcgagcg cgccctgccg gtcatgcaga gctatgcaaa
     6421 ggcctgcacc ctgatcggcc ccgtcggcag cgggcagctg gcaaagatgg tcaaccagat
     6481 ctgcatcgcg ggcatcgtcc agggactttc cgaagccttg cacttcgcga aacgcgcagg
     6541 gctcgacgtc gaagcggtta tcggggcgat ctccagggga gccgcgcaat cctggcagat
     6601 ggaaaaccgc tggaagacga tggtgaacga ccagttcgag ttcggcttcg ccgtcgagtg
     6661 gatgcgcaag gacttgaaaa ttgcgctgga cgaggccgcc cgcaacggcg caacgctcga
     6721 aatgaccgcg ctggtggaca aatattacaa ggaagtggaa gagatgggcg gtgcgcgctg
     6781 ggacacgtcc agcctgatcc ggcgtttgga gaaaaagtag cgcgcggttc tgcgcccact
     6841 tacgggagaa cccctggtag gagcccgcca tgcgggcgag tgcgcctagg tccatcgcac
     6901 tcacccgcat ggcgagctcc tacagtgcta tccatgttct gcgggaagct tgaagaacag
     6961 cccgctgaaa agggaagctg tatccggtat agtacgaagc gtcacagggg tggcctgatc
     7021 ggcctgagat ccggggcgtc gcaaggttgc gatgccggat aaccgggaac ccttagaacc
     7081 tgaaccgggt aataccggcg taggaagagg attctcccat gttccactcg atatcccggc
     7141 agggaagcgt attcgcttac cgcgccatga tccttttgtt tgccgccgtc gcgagcgcga
     7201 ccgccgacga agtactcgaa gaaatcatcg tcaccgccga ttttcgcgag cgtccggcgg
     7261 atgagatggc tgcaagcatc accgttctcg acaccgactc gatcgaacaa ctggctgtgc
     7321 aacatttcga ggagatggtg ttcttcgtgc cgaacctgaa ctggtccgga gacgggaatc
     7381 gcgcacgcta tttccagatt cgcggcgtgg gtgaactgga gcaatacgaa ggtgcaccga
     7441 acccttcggt cggtttcatc atcgacgaca tcgactttag tggcatcggg actatcgcaa
     7501 cactgttcga catcgagcag atcgaagtcc tgcgagggcc gcagggcagc cgctacggtg
     7561 cgcaggcgct ggccggtctc atctatgtgc agagcaccga accggacgac gaatggtcgg
     7621 gccgcgtgca ggtgggcggc gggcaggaca acgcgatgtc cggcggcgtg gcgctcggcg
     7681 gcccgctgac gaccgacggc gccttgagat tccggttgag cggacaccac catgagagcg
     7741 acggatttcg cgaaaactcc tacctcggaa gtagcgacac gaacggccgc aaggaatcca
     7801 cggctcgcgc gaaaatggca tgggatgctt ccgacgactg gcaactgaag ctcgcgacga
     7861 tgtggagcga catcgacgac ggctatgacg ggtttgtggt cgataactcc ctgacgatgc
     7921 tgtcggacaa gccgggacga gacgcgcaga gaagcctcgg tgccgctttt acagccgggt
     7981 ggtccggatc cgaccgcttc gatttcacgt ccatcacgtc cgcggcgaaa tcggacatcg
     8041 atttcagctt cgatgcagac tggggaaatc cggagtcgtg ggctcctttc acgtacgact
     8101 tcatatccgc caatgacagg gagcggcgca ccctgagcca ggagtttcga gtcgcagcgg
     8161 gccctgaggg caggttgttc aacggctcca ccgattggct catcggagcc tattacctga
     8221 agctcgagga cgaactcgcg accttgaacc agggcgaata ctttgatccg ctcgcaggct
     8281 tcgccgatag acttgacgat cgcttctcca gcagcttcga tgcctcgaac gtggccctct
     8341 tcgggcagat ggacatcgac gtcggggaat ccggcgaagt gggtatcggt ttgcgcgtcg
     8401 agaaccgcag cacggactac tttgattccg caggccttca gctcgatccc ggcgaaacga
     8461 tgcttggcgg cgagctgagc tacaggcata gcttcgacga cgggctttcc gcgtacgtaa
     8521 gcctgtcgcg cggctacaaa gccggcgggt tcaacctcgg tgtcgtgccg gagggacggc
     8581 gcgagttcgg acaggaagcc atgtggaaca ttgaaagcgg cctgaaagca ctgtggctgg
     8641 atgacacgct cctgttcaac ggcgcgattt tctacagccg aagggacgac cagcaggtca
     8701 ggacgtcggt gcagcttgcg ccgaacgatc ccgcttcatt tgttttcttc acagataacg
     8761 ctgccgaagg ccggatatca ggcctcgagg cggagttgcg ctggctcccg acggaaagcc
     8821 tggagttcta cacgaatgtt gggctgcttg acgcggaatt ctcggagttc gccgatgccg
     8881 agggccgttt gagcagccgc gaccaggcgc acgcaccgaa cttcacgctt gcgctcggcg
     8941 ggatgtaccg gcgcgccaac ggttttttcg cgcgcctcga tctttccgcg cgggatgagt
     9001 tctatttcga cgtcagtcac gaccagaaat cggaacgcca cgccctggcc aatgcgcgcg
     9061 tcggcttcga tacggagcgc tggacggcac aagtctggat gcgcaacgcg tttgacgaga
     9121 attacgcggt gcgaggtttc tacttcggga acgaaccgcc cgatttcccc acgaagctct
     9181 atgtgcgccg cggcgatcca cgccagctcg ggttcactgt cgatatgagg ttttaatgat
     9241 gcgtattgcc gtcgatatca gtctatatcc ccttagttcc gatttcctgc cgccgatcaa
     9301 ggatgtgatc tcacgcctga attcgtaccc ttcgctggaa gtcgagacca acccgatgag
     9361 cacgcagatc cgcggcgagt acgacgaggt gatgaacgcg ctgcagcagg agatgaaaca
     9421 gaccttcgaa gacgtaccga aagcagtttt cgccatccgc atcctgaaca accccatgag
     9481 cacgccgtag gagcccgcca tgcgggcgac aaccaccacc ttgcatcgca cgatggtcat
     9541 gatcgtgcgt agacagctcg catccaaact catatgaccg gcatcaccgc ccaattacag
     9601 gctctctccc ccgccgaggt aattgccgtc gccgccgctg tcgcctacct ctttttcgcc
     9661 atccgccaga acatcctctg ctggttcttc gcgctggtca gcaccgcgat ctacatcggt
     9721 ctcttcttcg gcgcgaagct gtacatggaa tccgtgctca atattttcta tttcggcatg
     9781 gcgctgtacg gctggtactt ctggctagcg ggcggcggca gggaatcgac tctcccggtg
     9841 accaggtggc cgctgctccc gcatgtcctg gccgttggcg gcatcctgct tctttcgctc
     9901 ctgttcggct cgctgctcga tcgctacacc caggccgcgt atccctacg
//
LOCUS       DASUOM010000255         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1599377,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000255 DASUOM010000000
VERSION     DASUOM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599377"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFY27_05305"
     CDS             complement(<1..775)
                     /locus_tag="VFY27_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEX5764965.1"
                     /translation="MDLSSTLATINALLWNETWLFVIVGTGLLFTLWSGFSQYRALTH
                     GPAVVRGVYDDPEDPGAINHFQALSAALSGTVGLGNIGGVALAIALGGPGAIFWMWVV
                     GLLGMAIKLTEVTLSMLYRNTDDPDNPHGGPMWVADKALARISPKYAWLGKVIAVIFC
                     ICLLMSASTGGNMFQAWNIAEVTNEYFGVPGWVSGVIMAAVVAAVIIGGIKRIGRVAG
                     ILVPFMVALYLIAGTYVLILNFSGIPGMLLMIVANAFTPT"
     gene            complement(848..2143)
                     /locus_tag="VFY27_05310"
     CDS             complement(848..2143)
                     /locus_tag="VFY27_05310"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5764966.1"
                     /translation="MWHDGRAIRIAVVGGLRIPFCRAYTSYAGCSNQDMMTATLAAIV
                     QKFDLKGQVLGDVALGAVIKHSRDWNLARECVIGSGLSLKTPGVDLQRACGTSLEACI
                     LIGNKIALGQIEAGVAGGTDSISDAPVVYPDEYRSILMQSHRGKGMKDKLAPWLKLRP
                     RHFKPVLPGVTEPRTGLSMGEHTEITAKEFQVSREHQDQLALASHRNAAKAYDSGFYS
                     DLVVPFRGVREDNNIRRDTSFDKLAKLKPVYDRSGSGTMTAGNSTPLTDGAAAVLLSS
                     EAWAGKKNLPVYAYLTYCKVSAVNFTGQEGLLMAPVYAVSEMLKVANLKLQDFDYYEI
                     HEAFAAQTIATLKAWQSEEFCREKLGRNEPLGPIDLAKLNVKGGSIAIGHPFAATGAR
                     VVATMARLLHEKGSGRGLISVCTAGGMGVTAIMEAASKA"
     gene            2281..>2472
                     /locus_tag="VFY27_05315"
     CDS             2281..>2472
                     /locus_tag="VFY27_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5764967.1"
                     /translation="MTTFASTGSWYAETKALLILAAPLIVNSLSIAGMQFADAVMAGR
                     LGAEALAAVAVGTSVWFLGF"
BASE COUNT          450 a          774 c          761 g          487 t
ORIGIN      
        1 cggtaggcgt gaacgcgttg gcgacgatca tgaggagcat tcccgggatt cccgaaaaat
       61 tgaggatgag cacgtaggtg cctgcgatca ggtagagcgc gaccatgaag ggcacgagga
      121 tgcccgcgac cctgccgatg cgcttgatgc cgccgatgat taccgccgcc acgaccgcgg
      181 ccatgattac tccggaaacc catccaggta cgccgaaata ctcattggtg acttccgcga
      241 tgttccatgc ctggaacatg ttgccgccgg tagatgccga catgagcagg caaatgcaga
      301 agatgacggc gatgaccttg ccgagccagg catatttcgg gctgatgcgc gcgagcgcct
      361 tgtccgcaac ccacattggc cccccatggg ggttgtccgg atcgtccgtg ttccggtaga
      421 gcatggagag cgtaacttcg gtgagcttga tcgccatgcc gaggaggccg accacccaca
      481 tccagaaaat cgctccggga ccgcccagcg ctatcgcgag cgcaacgccc ccgatgttcc
      541 cgagccccac cgttcccgaa agggcagccg agagcgcctg gaaatggttg atggccccgg
      601 gatcttcggg atcgtcgtaa acgccccgca cgaccgccgg cccgtgggtg agggcccggt
      661 attgcgagaa gccgctccat aacgtgaaca gaagcccggt gccgacgatg acgaagagcc
      721 aggtttcatt ccacaaaagc gcgtttatgg tcgcgagtgt cgagctgaga tccatcgttt
      781 ccccgtcagc gtacgcgagc tgccagcgta cttctgcgac ctgccgccga atggaccgtc
      841 gcctgtgtta tgccttcgat gcggcttcca tgatcgcggt gacgcccatg ccgcccgcgg
      901 tacagacgga gatgagaccg cgcccggaac ctttctcgtg cagcagcctg gccatggttg
      961 ccacgacccg cgcgccggtt gccgcgaagg gatgccctat cgcgatgctg cctcccttca
     1021 cgttgagctt ggccaggtcg atcggcccca gcggctcgtt gcgccccagt ttctccctgc
     1081 agaattcctc cgactgccac gctttcaggg tcgcgatggt ctgcgcggcg aaagcctcgt
     1141 ggatctcgta gtagtcgaag tcctgcaatt tcaggttcgc caccttcagc atttcggaga
     1201 ccgcgtagac cggtgccatc aggagacctt cctgccccgt gaaattgacc gcactgacct
     1261 tgcagtaagt gaggtatgcg tatacgggca ggttcttttt ccctgcccac gcctccgacg
     1321 acaagaggac tgcggcggcg ccgtcggtca gcggagtgct gttccccgca gtcatcgtcc
     1381 cgctgccgct cctgtcgtaa acgggtttga gtttcgcaag cttgtcgaag ctcgtgtcgc
     1441 gccggatgtt gttgtcctcc ctgacgccgc gaaacgggac gaccaggtcg gaatagaagc
     1501 cgctgtcgta agccttggcc gcgttacggt ggctggcgag cgcaagctgg tcctggtgtt
     1561 cgcggctcac ctggaattcc ttcgccgtga tctcggtatg ctcgcccatc gagagaccgg
     1621 tgcgcggctc ggtgacgccg ggaaggaccg gcttgaagtg ccggggccgc agtttcagcc
     1681 acggcgccag cttgtctttc atccctttgc cgcggtggct ctgcatgagg atagaccggt
     1741 attcgtccgg gtacacaacg ggggcgtcgc tgatcgaatc ggtgccgccg gccactcccg
     1801 cttctatctg tccgagcgcg atcttgttgc cgatgaggat gcaggcctcg aggctggtgc
     1861 cgcaggcccg ctgcaagtcg acgccgggcg tcttcagtga caggcccgag ccaatgacgc
     1921 actcccgggc gaggttccag tcacgggaat gcttgatcac ggctccgaga gcgacatcgc
     1981 cgagaacctg tcccttcaga tcgaatttct ggacaatggc cgccagtgtc gccgtcatca
     2041 tgtcctggtt ggagcaaccg gcataactgg tgtatgcgcg gcagaacgga atacgcaaac
     2101 ccccaacgac cgcgatcctg attgcccggc catcgtgcca catcatcgcc tccggatgtt
     2161 ggtgagccgg cccaacttta ctatagggca ggcttccatg cctgatattg cagcaattta
     2221 ccgtatcgta cgcatgccgg agccgcgccg cgaaaacgcg ccgtatgaac aagaacacga
     2281 atgacgacat ttgcatccac aggtagctgg tacgcggaga cgaaggcgct cctgatcctt
     2341 gctgcgccgc tgatcgtcaa cagtctttcg atcgcgggca tgcagtttgc cgatgccgtg
     2401 atggcaggga ggctcggcgc cgaggcgctt gccgcggtcg ccgtcggcac gagcgtctgg
     2461 ttcctcggtt tt
//
LOCUS       DASUOM010000256         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_137191,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000256 DASUOM010000000
VERSION     DASUOM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137191"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFY27_05320"
     CDS             complement(<1..585)
                     /locus_tag="VFY27_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764968.1"
                     /translation="MAGCGGSSDPSPLHFQFTFAEDLEDWAVGFADYPAGTSDAEQQA
                     IDEFYEIDGRHARLPQPLDTGDGAVLLTGNNHSDDLFMFLKRRVSGLAPDTTYQLELR
                     VVFATDAPRGCAGIGGSPGESVFVKGGASGIEPLRVMDGTAGHATWIMNVDKGNQGSG
                     GTDAQVLGDFANLEDCESSDFTYQLKELSNLELPE"
     gene            856..1335
                     /locus_tag="VFY27_05325"
     CDS             856..1335
                     /locus_tag="VFY27_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764969.1"
                     /translation="MQRMDIELWIFLSYVVTVIGFPLAIAVFLYEKRKERVLEEEEVF
                     ELLSGSYQDFLKIALENPDLRLLSKARTRDLTEEQQERMRAIFGLLMALFERAYLLLY
                     DPGSKGKQARRWRSWEDFMQEWCERGDFREALPELLRGEDPEFAAYIRKLADVALQG"
     gene            complement(1480..2532)
                     /locus_tag="VFY27_05330"
     CDS             complement(1480..2532)
                     /locus_tag="VFY27_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5764970.1"
                     /translation="MLYEPTSLASLTGLLVTALRDHYGIDPAALFSHAGIPLVPEHLP
                     QHRYPLSTIRKLWEVSKRATGDDTIGLKAGTYATPAHFYAFGYSWMASSTLLEAMQRL
                     VRYHKLVSTASFVVALKGAGGSFALSAEFPDESRSPPRESIDCGMTSLLALCDIITEK
                     EIRPSRIELTCPATLHPDAYRQALRAPIKFSAKVGTFYFSRELLMERLRHGAPDIAYA
                     TDTIAERYIESLDRHKVASQVRELLIKLLPSGGADQVSVSRRLNRSASTLQRQLQDEG
                     LTYREVLDTTRRRLAEAYLTDGQLSVAQVAYMLGFSDQSNFSRAFRRWTSKTPKQYQA
                     GRPRFESARLRQIKRG"
     gene            2627..2989
                     /locus_tag="VFY27_05335"
     CDS             2627..2989
                     /locus_tag="VFY27_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764971.1"
                     /translation="MEEPTGDDSLAIADAVARAAHIWIRSLCLNEFQEADERRSMASG
                     LIAGLCERLDVDPRIHQLITYIYALLDNDADGSLDSTRDILQKAMAPENLSARQRGVQ
                     EAEAIVELFVYHRNRIPD"
     gene            3191..4405
                     /locus_tag="VFY27_05340"
     CDS             3191..4405
                     /locus_tag="VFY27_05340"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX5764972.1"
                     /translation="MTAIAQRSIFGLVSVAWVFAQAAAQTPAAETPTTAAGFTEEQLE
                     QIVAPIALYPDPLVSQMLMASTYPLEIVEAARWLKANPGLKDTALEEALKKESWDASV
                     KSLCGFPDVIEQMNENLDWTQDLGDAFLAQKTELLDTVQRMRGKAYDAGNLTTTEQQV
                     VTQQPDKIIVIEPASPEIIYVPTYSPTVVYGSWSYPHYYYPPMYVPPPYGYGAMAFTV
                     GVVWGAAIWGGCNWGWGSSDVDIDINRQNNFNRNTNIDGSRNNINNKAGGRQSWQHDA
                     SHRNGVNYRDSKTAQQYGASAGSNRVPRSQARGYERAGTGNMSAGQRGGPGTSTGTAG
                     SRDAGASRGSSASSRDLSASSRGSGASSRGSWDSSAYSGSRNTSFDHSASNRGGMSRG
                     GGMSRGGGSRRR"
BASE COUNT          945 a         1327 c         1369 g          903 t
ORIGIN      
        1 ctccggcaac tccaggttcg acaattcttt cagttggtat gtgaaatccg acgactcgca
       61 gtcctccaga ttggcgaagt cgccgagaac ttgcgcgtcc gtgccgccac ttccctgatt
      121 gcccttgtcg acgttcatga tccatgttgc gtgacccgct gtaccgtcca tgacgcgtag
      181 cggttcgatg ccgctcgccc ctcccttcac gaacacactt tcaccagggg atccgccgat
      241 gccggcgcag cctctcggcg catcggtcgc aaataccacg cgcagctcga gctggtaggt
      301 cgtgtccgga gccagtccgc tgacgcgtcg tttcaggaac ataaaaaggt cgtcggaatg
      361 attattaccg gtgagcagca cggctccgtc gccggtatcg agtggctgcg gcaggcgcgc
      421 gtggcggccg tcaatttcat agaactcatc gatcgcctgt tgctcggcgt cgctcgttcc
      481 cgcggggtaa tcggcgaagc cgacggccca gtcttcgaga tcctcggcaa aggtaaattg
      541 aaaatgcagg ggagaggggt cggaactgcc gccgcagccg gccactgtca agcctgccat
      601 caccagtaat gctctggtgg cggaccgcat ttgagtaggc atttagacgg cacccgtcaa
      661 aagaacgaaa gctagaccat ggcggacgcc cccgagttcc gcagcaaggc tgcgaagcag
      721 ctaggtacgg aactgacagc agtttcagcg ttcgtctgca gaccaaggca ggccgcggcg
      781 cggcgcccct cgatcagatc agagtcagag ccgcatcatg cgccggggca cctgccttgg
      841 agccgtgctg gcataatgca gcgcatggac atcgagttgt ggatattcct cagctacgtg
      901 gtgaccgtca tcggctttcc gctggcgatc gcagtttttc tctatgaaaa gcgcaaggaa
      961 cgggtactcg aagaggaaga ggttttcgaa ctcctttccg gtagctacca ggacttcctg
     1021 aagattgcgt tggagaatcc ggacctgcgc ctgctttcga aagcgcgcac gcgggacctg
     1081 acggaggagc aacaggagcg catgcgggcg atcttcggcc tgctgatggc actgttcgaa
     1141 cgtgcctacc tgctcctcta tgatccggga tcgaagggga agcaggcacg gcgctggcgt
     1201 tcctgggagg actttatgca ggaatggtgc gagcgcggcg acttccgcga ggcgcttccg
     1261 gaactcctgc gcggggaaga tcctgagttc gccgcgtata tacgcaaact ggcggatgtt
     1321 gccctgcagg gctgactgcg cgaggcagtc attggatcgc ctcgaacaag gtgaataccg
     1381 gctgcaggcg tattgcacct cagcgtattg cacctcagat ggaatcggga agtgcaaatc
     1441 gggacgttgc aaaccggcgg accggtctcg accttccgat caccctcgtt tgatctgcct
     1501 gagtcttgcc gattcaaatc tgggtctgcc tgcctgatac tgcttcggcg ttttcgatgt
     1561 ccagcgcctg aatgcacgag agaaattgct ctggtctgaa aaccccagca tgtatgcaac
     1621 ctgtgccaca gacagttgtc cgtccgtcag ataggcctca gccaggcggc gacgagtggt
     1681 atccagcacc tcccgatacg tgagcccttc atcctgtaat tgccgctgta atgtactcgc
     1741 gctacggttg agacgcctgg ataccgacac ctgatccgct ccccctgaag gcaaaagttt
     1801 gatcagcaac tcgcgtacct gcgatgccac tttgtgccgg tcgagagatt ctatgtaccg
     1861 ttccgcaatc gtgtcggtcg cgtaagcaat atccggtgcg ccgtggcgca gtcgttccat
     1921 cagcagctca cgggagaaat agaacgtgcc taccttggca ctgaatttga tgggcgcacg
     1981 gagcgcttgt cgataggcat ccggatgcag tgtcgcagga cacgtaagtt ctatgcgaga
     2041 cggccggatt tccttttccg tgatgatgtc gcaaagagcc agcaatgaag tcattccgca
     2101 atcgatgctt tctcttggcg gactcctgga ttcgtccggg aattccgccg acagtgcgaa
     2161 gctgccacct gctcctttca acgcgactac aaaagaggcg gtcgacacca atttatggta
     2221 gcggaccagt cgttgcattg cttcgagcaa agtcgaactg gccatccacg aatagccgaa
     2281 cgcataaaaa tgtgccggcg tcgcgtaagt accagccttc aggccaatag tgtcatcccc
     2341 tgttgcccgc ttgctgacct cccaaagttt gcggatcgtc gacagcggat agcggtgttg
     2401 tggcaaatgt tccggcacta acggaatccc ggcatgggaa aacaacgcgg ccggatcgat
     2461 gccgtagtgg tcccgcaatg cagtcaccag caagccggtc agggacgcga gtgatgttgg
     2521 ctcatacagc attagcggca atttgaatgg agattggtgt gtatcgacaa gagccgacag
     2581 tcgatgttgc cgaatatgcc ggccatcagg gtcatggagc aaagtaatgg aagagccgac
     2641 aggcgatgat tcgctggcga ttgcggatgc tgtcgcccgc gccgcacaca tctggattcg
     2701 ttccctctgc ctgaatgagt tccaggaggc agatgaacgc cgctccatgg cttccggact
     2761 gatcgcgggg ttgtgtgaga ggctcgatgt cgatcccagg atccaccaac tgatcactta
     2821 catttacgcg ctcctcgaca acgacgctga cggttccctc gactcgacac gagacattct
     2881 tcagaaggcg atggcaccag agaatctttc cgcacgccaa aggggtgtgc aggaagcgga
     2941 ggcaattgtc gaactgttcg tttaccacag aaacaggata cctgactagg accatggtcc
     3001 cactggaacg cgccgtttcc agcggtaccc tgcaagccgg tagcgctccc ggcagggcgc
     3061 gttcctctgt ccatggaagg gaaacgcgat tagaggcgtt tgagcgacgc cacttcccgc
     3121 agccgaattt ccataaggcg acagctgcaa ccagacatct acctggtacg gcgcatttgg
     3181 aggggtttcc gtgacggcaa tcgctcaacg ctcgatcttc ggactagtgt ccgtcgcatg
     3241 ggtgttcgcg caggccgcgg cccagacgcc ggcagccgag acgccgacaa cggcagcggg
     3301 cttcaccgag gaacagctgg aacagatcgt ggcgccgatt gcgctctatc cggacccgtt
     3361 ggtgtcgcag atgctgatgg cctcgaccta tccgctcgag atcgtcgagg ccgcccgctg
     3421 gttgaaagcg aatccggggc tcaaggacac agcgctcgag gaggcgctca aaaaggaatc
     3481 ctgggacgcc agcgtcaaat ccctgtgcgg atttccggac gtgatcgagc agatgaatga
     3541 gaatctcgac tggacccagg atctcggtga cgcttttctg gcgcagaaaa ccgagttgct
     3601 cgacaccgtt cagcgcatgc gcggcaaggc ctatgatgcc ggcaatttga cgaccacgga
     3661 gcagcaggtt gtcacgcagc agccggacaa gatcatcgtg atcgagcccg ccagtcccga
     3721 gatcatctac gttccgactt actcgccgac ggtcgtttac ggatcgtggt cttaccctca
     3781 ctactactat ccgccgatgt atgttccccc gccctacggc tacggtgcga tggcattcac
     3841 cgtgggcgtg gtgtggggtg ccgcgatctg gggggggtgc aactggggtt ggggcagcag
     3901 tgatgtcgat atcgacatca atcggcagaa caacttcaat cgcaacacga acatcgacgg
     3961 cagcaggaac aatatcaata acaaggctgg tggcaggcag tcgtggcagc acgatgcctc
     4021 gcaccgtaac ggcgtcaact atcgcgacag caagacggcg cagcagtacg gagcgagtgc
     4081 cggatcgaac cgtgtcccgc gcagccaggc gcgcggttac gaacgcgcgg gcacaggcaa
     4141 tatgtctgcc ggtcagcgcg gcggccccgg cacgtcgacc ggtacagcgg gaagccggga
     4201 tgcgggcgcc tcgcgcggtt cctcggcttc ttcccgagac ctttcggctt catcgcgcgg
     4261 ctccggcgct tcgtcccgtg gtagctggga cagtagcgcc tacagcggct cgcgcaacac
     4321 gagcttcgat cattctgcga gtaaccgcgg cggcatgagc cgtggcggcg gcatgagccg
     4381 tggcggcggc tcgcggcggc gataaaagat cagggagaaa tgcgatgatt acgcctgttc
     4441 agctgcgtca tttctgcctg gcaattgccg gcatgatcgt cactgccctc gcagcatgct
     4501 cacccgggcc ggccggcact tacaaaactc ccgaagaagc cgtt
//
LOCUS       DASUOM010000257         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_202987,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000257 DASUOM010000000
VERSION     DASUOM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202987"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFY27_05345"
     CDS             complement(<1..398)
                     /locus_tag="VFY27_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5764973.1"
                     /translation="MTSVLINLLSFMVAISVLVAIHEFGHYLVGRLCGMKVLRFSIGF
                     GKPLWTWIAGKDRTEYCISALPLGGYVKFLDEREGPVEPEDRGRAFNHRPVPARIAVL
                     FAGPLFNFLFAIVAYWVLFLNGIPTIKPAVG"
     gene            complement(463..1257)
                     /locus_tag="VFY27_05350"
     CDS             complement(463..1257)
                     /locus_tag="VFY27_05350"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5764974.1"
                     /translation="MLLQRILTAIVAALLILLVLFAFPPLAAKAVIASIILAAAWEWA
                     KFLSLVTVGERLGYVATIALLLAGVTWLTPSRDAAEFILKVGLAWWFAALVWAFFYPT
                     PISRPVGWICGALVLVPAFVALVLLYSLRPSVLLFALIIIWAADTGAYFAGRRFGRIK
                     LAPSISPKKTWEGVIGGLAAVVLVLIAARLYWLEIDIAVIIPFCLAVATLSVVGDLTV
                     SMFKRNAGLKDSGRVFPGHGGVLDRIDSVTAAAPLFALGISWAGLI"
     gene            complement(1257..2012)
                     /locus_tag="VFY27_05355"
     CDS             complement(1257..2012)
                     /locus_tag="VFY27_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5764975.1"
                     /translation="MRDPAKAVDRSPARVPAHVAVIMDGNGRWAKMRSEPRHAGHRAG
                     ARAVRNTVETAARRGVSYLTLFAFSSENWRRPAEEVSSLMTLFLEALQREIDDLHRNN
                     VRLNFVGERLRLQSRLQQKIKAAEARTAENDGLTLTVAVAYGGRWDIVNAARELAAQV
                     ERGEIRSSDIDEERFGGQLALAGVPDPDLLIRTGGEQRISNFLLWNLAYAELYFCQCL
                     WPDFDEKEFDRALHMYAERQRRYGHTGEQVGAH"
     gene            complement(2079..2636)
                     /gene="frr"
                     /locus_tag="VFY27_05360"
     CDS             complement(2079..2636)
                     /gene="frr"
                     /locus_tag="VFY27_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5764976.1"
                     /translation="MLEDIKKDAAKRMDKSIESLRQEMTKLRTGRAHTSLLDHITVEY
                     YGSQVPLSQVSNINVEDSRTLLVSPWEKTMVQAVERAIMHSDLGLNPVSAGTVIRVPL
                     PPLTEERRKDMIRLVRQEAEGGRVAIRNIRRDAINDLKDLMKEKLIGADEERRGQEEI
                     QAITDKHIAEIDRVLAEKERDLMEV"
     gene            complement(2727..>2997)
                     /locus_tag="VFY27_05365"
     CDS             complement(2727..>2997)
                     /locus_tag="VFY27_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5764977.1"
                     /translation="AIEIGADVLLKATKVSGVYDSDPKENSEASLYDTVSFDQVLIDK
                     LNVMDATAVVMCRDNHLPLRVFNMTEEGALVKAMTDENVGTLVTL"
BASE COUNT          571 a          982 c          885 g          559 t
ORIGIN      
        1 ccgacggccg gctttatcgt cgggatgccg ttcaggaaca gcacccagta agcaacgatg
       61 gcgaacagga aattgaaaag cgggccggcg aagagcactg cgatgcgcgc aggcaccggc
      121 cggtggttga atgcgcgacc ccggtcctcc ggctcgaccg gcccctcacg ttcgtccagg
      181 aactttacgt aacctcccag cggcagcgct gatatgcagt attcggtgcg gtccttgccg
      241 gcgatccagg tccagagcgg cttcccgaaa ccgatggaga acctcaagac cttcatgccg
      301 cacaggcgcc cgacgaggta atggccgaac tcgtgtattg caaccagtac gctgatagcc
      361 accatgaaag acagcagatt gatcaatacg gaagtcatgt acgacgaaac caggtggcta
      421 tgccgtaatt ctgactggca gccgcgaacg cagttcccgc catcagatca gcccggccca
      481 gctgatcccg agggcaaaga ggggcgctgc cgccgtgaca ctgtcgatgc gatcgagcac
      541 gccgccgtgc cccgggaaca cccgaccgct gtccttcagg cccgcgtttc gcttgaacat
      601 gctgaccgtc aggtcgccga ccacggacaa agtagcaacg gccaggcaga acgggatgat
      661 gacggcgata tcgatctcga gccagtagag gcgcgctgcg atgagcacca gcaccacggc
      721 tgccaggccg ccgatcacgc cttcccaggt tttcttcggg ctgatgctcg gcgccagctt
      781 gatgcggccg aatcgccttc cggcaaagta cgctcctgta tcggctgccc agatgatgat
      841 cagcgcgaac aggagcaccg agggccgcag cgagtacagc aagaccagcg cgacgaatgc
      901 cggcaccagc accagcgcac cgcaaatcca gccgaccggc cgggaaatcg gcgtcgggta
      961 aaaaaacgcc cagaccagtg ccgcaaacca ccaggcaagc ccgactttca ggatgaactc
     1021 ggcggcgtcc cgtgacggcg tgagccacgt gacgccggcg agcagcagcg caatggtggc
     1081 cacgtaaccc agccgctctc ccacggttac gagggagagg aactttgccc attcccacgc
     1141 cgccgcgaga atgatcgacg caatcactgc cttcgcggcc aggggcggga acgcgaacag
     1201 cacgagcagt atcagtaagg cggcgacgat tgcagtgagg atgcgctgca gcaacatcag
     1261 tgcgccccga cttgttcgcc ggtatggccg tagcgccgct gccgctcggc gtacatgtgc
     1321 agcgcccggt cgaactcctt ctcgtcgaaa tccggccaca ggcactggca gaagtagagc
     1381 tccgcataag ccaggttcca cagcaggaaa ttgctgatgc gctgctcacc gcccgtccgg
     1441 atcaggagat cgggatccgg aacgccggca agggccagct ggcccccaaa tctctcctcg
     1501 tcgatgtccg atgaccggat ctccccgcgc tccacctgcg ccgccagctc ccgcgcggca
     1561 ttgacgatat cccatcggcc gccataggcg acggcgacgg tgagggtaag cccgtcgttc
     1621 tccgcggtac gggcttcggc agccttgatc ttctgctgca gacggctttg cagccgcagc
     1681 cgctcgccga caaaattgag ccttacatta ttacgatgca ggtcgtcgat ttcccgttgc
     1741 agtgcctcca ggaaaagtgt catcaggctg ctgacttctt cggccggccg ccgccagttc
     1801 tcgctgctga aggcaaacag ggtcaggtat gaaacgcctc ggcgtgccgc tgtctccacc
     1861 gtattgcgca cggccctggc gcctgcgcga tgcccggcgt ggcgcggctc agaacgcatc
     1921 ttcgcccagc gcccgttgcc gtccatgatg accgccacgt gagccggtac gcgcgctggc
     1981 gaacgatcga cggctttcgc gggatcgcgc aagctaaagc gcccggtcgc aaataatgag
     2041 ccatcctcgc gggtgaattc tcgtggctgt caagggcact atacttccat caggtcgcgt
     2101 tctttctcgg cgagcacccg gtcgatctcc gcaatgtgct tgtccgtgat ggcctgtatc
     2161 tcttcctggc cgcgccgctc ttcatccgcg ccgatgagtt tctccttcat caggtctttg
     2221 aggtcgttga tggcgtcgcg gcggatgttg cggattgcaa cccgaccgcc ctctgcttcc
     2281 tggcgcacga ggcgaatcat gtctttgcgg cgttcctccg tcagcggagg taacggcacc
     2341 cggatgaccg ttcccgcgga aaccgggttc agcccgaggt ccgaatgcat gatcgctctc
     2401 tctaccgcct gcaccatggt tttttcccat ggcgatacga ggagcgtgcg ggaatcctcg
     2461 acgttgatgt tcgatacctg gctcagcggc acctggctcc cgtaatactc cacggtgatg
     2521 tggtcgagca ggctggtatg cgcccgtccc gttcggagct tcgtcatttc ctggcgcaac
     2581 gattcgatgc tcttgtccat gcgcttggcg gcgtcttttt tgatgtcttc aagcacgttt
     2641 cgctcctcct gcaacttgca tcaggcacca ggctgccacc aggcctcctg ggcgctcttc
     2701 ggtcccgacg cgccggccac ccggcgtcag agagtcacca gtgtaccgac gttctcatcg
     2761 gtcatggctt ttaccagggc accctcctcc gtcatgttga acacccgcaa tggcaggtgg
     2821 ttgtcccggc acatcacgac cgcggtggca tccatcacgt tcagcttgtc gatcagcacc
     2881 tggtcgaagg acaccgtgtc gtacagcgag gcctcggaat tttccttcgg atcggagtcg
     2941 tagacaccgc tgaccttggt agccttgagc agaacgtccg caccgatctc gatggca
//
LOCUS       DASUOM010000258         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_894930,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000258 DASUOM010000000
VERSION     DASUOM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894930"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFY27_05370"
     CDS             <1..263
                     /locus_tag="VFY27_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764978.1"
                     /translation="NRRGECYLDEDKRANGFQSIWGRFMTRVLKETDLEQRFAERDIR
                     AKTASDADSLQRAQEILGHADAGITKRVYVRKPQLVCPGKGI"
     gene            complement(327..403)
                     /locus_tag="VFY27_05375"
     tRNA            complement(327..403)
                     /locus_tag="VFY27_05375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Arg,seq:acg)
     gene            complement(425..517)
                     /locus_tag="VFY27_05380"
     tRNA            complement(425..517)
                     /locus_tag="VFY27_05380"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Ser,seq:gct)
     gene            complement(842..1048)
                     /gene="csrA"
                     /locus_tag="VFY27_05385"
     CDS             complement(842..1048)
                     /gene="csrA"
                     /locus_tag="VFY27_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX5764979.1"
                     /translation="MLILTRRAGETVMIGSDVTITVLGVKGNQVRIGINAPKDVAVHR
                     EEIYERIQSEKSARDAEKPGAQEN"
     gene            complement(1172..3787)
                     /gene="alaS"
                     /locus_tag="VFY27_05390"
     CDS             complement(1172..3787)
                     /gene="alaS"
                     /locus_tag="VFY27_05390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5764980.1"
                     /translation="MTSNELRLAFLEFFRSRGHEVVPSSPLVPGNDPTLLFTNAGMVQ
                     FKDVFLGQDKRRYDRAVTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     SQAIAFAWEFLTKDLGLPPERLWITVFEDDDEAAAIWLNEVGIDPQRFSRLGAKDNFW
                     AMGDTGPCGPCSEIFYDHGPDVPGGPPGSPDEEGDRYVEIWNLVFMQFDRSGSGEMTP
                     LPKPSVDTGMGLERMAAVMQGVHSNYEIDLFANLIEATAKILGVANDGSSSLNVVADH
                     IRACAFLVTDGVLPGNEGRGYVLRRIIRRAIRHGKKLGATESFFHRLVGPLCKEMGDA
                     YPELVKARAHVEKVLAKEEARFAETLDQGMQILEDAIAGLQGKELPGDVVFKLYDTYG
                     FPVDLTADVARERGLVVDEEGFEAAMMAQRDRARAASKFGSEASSSIQVDEQSEFSGY
                     DRIKEESTVEALLREGQRVEVLEDGEHGAVVLASTPFYGESGGQVGDTGVLEGDHGSF
                     VVDDTQKSGKAILHLGRVQLGKLQVGDRITGRVDGERRGRIVLNHSATHLLHAALRKI
                     LGEHVMQKGSLVAPDRLRFDFSHYEALSPEQVQQIEKLVNDQIRRNTAAETNVMNYND
                     ALASGAIALFGEKYGDEVRVMRLGDFSVELCGGTHVKSAGEIGVFKITSEGGVASGIR
                     RIEAVTGKGALEWIDENQQALENVASLLRTQRSQAPAKVEQLLKRTRKLERELATTRQ
                     QLASGNTVRVEENIIEVEGIKVLASRMDGADAKTLRAAIDKYKDRLQNAVVVLASVEN
                     GKVRLAAGVTKNNLDRVRAGDVIRPVAEQVGGRGGGRPDFAEAGGSNPEALDAALDSV
                     PGWLAEHLGQVARPV"
BASE COUNT          689 a         1261 c         1080 g          839 t
ORIGIN      
        1 gcaaccgccg aggcgagtgc taccttgacg aggacaaacg ggcgaacggc ttccagtcca
       61 tctgggggcg attcatgact cgagtactga aagagacgga cttagagcag cgcttcgccg
      121 agcgtgacat acgggccaag acggcgagcg acgctgactc attgcagcgt gctcaggaga
      181 ttctgggcca cgctgacgca gggatcacga agcgcgttta tgtccgcaaa ccgcagcttg
      241 tatgcccggg aaagggcatt tgaatagcac agcgcccgat ttatagcaca gtgccgtgtt
      301 tcggtcgctt gtaaactgtt gaaaaatggc gcgcccggag agattcgaac tcccgaccgc
      361 ctggttcgta gccaggtact ctatccaact gagctacggg cgcgttgttt ggtatcaatt
      421 gtggtggcgg agagagaggg attcgaaccc tcggtacagc tttacactgt acaacggttt
      481 agcaaaccgc cgctttcgac cactcagcca tctctcctta aaaccttttc ctgatctgaa
      541 gcccgagaga gggatagact cgggccgtcc cggccctcgc ccttcgcgcc agcttcgctg
      601 ttcaaaatcg ctcccggcga ttttgtcgac cgctcagcca tctctcctta ataactctcc
      661 tgggagtgac cgcgattggt tcggtgcgtc ctacgccgca ccccgcggcc atttcgcgat
      721 ccagatgccc gcctttaggc ccgctcaggc cccccttcgg tcgaaactcg ctgcctgagc
      781 ccggcggcgg gcccgccatc ttacttagcg gctaacgatt ggtaaagccc gaaaatccgg
      841 gctaattctc ctgtgcgccg ggcttttcgg cgtccctggc actcttttcg ctctggatcc
      901 gttcgtatat ctcttcccga tgaacagcca catccttcgg ggcattgatg ccgatgcgaa
      961 cctgattccc tttaacgccc aaaacagtga tcgtgacatc gctcccgatc atgactgtct
     1021 cgccggcgcg tcgcgtaagg atcagcatga cttcctactc ctgcctcaat agatttcgcc
     1081 ctttcgtact acgtccatat gcccgtggtt atgttagacg aaatatagcc gggatgcgcg
     1141 tcgcgcccgg tcggtgggtt cggaagtgag gttagacggg ccgagctacc tgccccaggt
     1201 gctcggcaag ccagcccggg accgagtcga gtgctgcgtc cagggcttcc gggttgctgc
     1261 cgccggcctc ggcaaagtcg ggacgtccgc cgcctcttcc gcccacctgc tccgcgaccg
     1321 ggcggataac atcgccggca cggaccctgt cgaggttgtt cttcgtgacg ccggctgcca
     1381 gccgcacctt gccgttttcc accgacgcca ggaccacgac agcgttctgc aggcgatcct
     1441 tgtacttgtc gatggcagcg cgcagcgtct tcgcatccgc gccatccata cgcgacgcga
     1501 gcaccttgat gccctcgacc tcgatgatgt tctcctcgac tctgacggtg ttgccactcg
     1561 caagctgctg cctggtcgtc gcaagctccc gttcaagctt cctggtccgc ttcagcagct
     1621 gttccacttt ggccggtgcc tggctgcgct gcgttcgcag gagcgacgca acattttcca
     1681 gcgcctgctg attctcatcg atccattcga gcgcaccctt ccccgtcacc gcttctatac
     1741 ggcgtatgcc tgacgcgacc ccgccttccg acgtaatctt gaacacaccg atctcaccgg
     1801 cgctcttcac atgcgtgccg ccgcacagct ccaccgagaa atcgcccagc cgcattaccc
     1861 tcacttcatc gccgtatttc tcgccgaaga gagcaatcgc gccagaggcc agggcgtcgt
     1921 tgtaattcat tacgttcgtt tcggctgcgg tgttgcgacg tatctggtcg ttcaccagct
     1981 tctctatctg ctgcacctgc tcaggcgata gcgcttcata gtgggaaaaa tcgaagcgca
     2041 ggcgatcggg cgcaacgagt gatccttttt gcatgacgtg ctcacccagg atcttgcgca
     2101 aagcagcgtg cagcaggtgc gttgccgagt ggttcagtac tatcctgccg cgccgttcgc
     2161 catcgacacg ccccgtgatc ctgtcgccca cctgcagctt cccgagctgg acgcgtccaa
     2221 ggtgcaggat cgccttgccg gatttctgcg tgtcgtcgac gacgaaggat ccgtggtctc
     2281 cctcgagcac gcccgtatcc ccgacctggc cgccgctctc tccatagaaa ggcgtcgatg
     2341 caagaactac cgcgccatgc tcgccgtcct ccagcacctc gactcgctgc ccttcgcgca
     2401 acaaggcctc gacagtcgac tcctctttga tacggtcata gccgctgaac tcgctctgtt
     2461 cgtcgacctg gatgctcgaa gatgcctcac taccgaattt gctggcagca cgcgcgcgat
     2521 cacgttgtgc catcatcgcg gcctcgaaac cttcctcgtc taccacgagc ccgcgttcgc
     2581 gcgcgacgtc cgcagtcagg tcgaccggaa agccataggt gtcgtacagc ttgaacacca
     2641 cgtcgcccgg gagttccttg ccctgcaatc cggcgatcgc atcctcgagg atctgcatcc
     2701 cctggtcgag agtctcggcg aacctggcct cttccttcgc gagcaccttt tcgacgtgtg
     2761 ctcttgcctt taccagttcc gggtaagcgt cgcccatctc cttgcacagc ggaccgacca
     2821 gcctgtgaaa aaaactttcc gtcgcgccga gctttttgcc gtgacgtatt gcgcgccgga
     2881 tgatgcgcct cagcacgtag ccgcggcctt cgttcccggg cagtacgcca tccgtcacca
     2941 ggaatgcgca ggctcgaata tggtcggcga ccacgttcag cgagctcgag ccgtcgttgg
     3001 caacgccgag gattttggcc gtggcttcta tcaggttcgc gaacaggtcg atttcgtagt
     3061 tgctgtgcac gccctgcatc acggcagcca tgcgctcgag tcccataccg gtgtcgaccg
     3121 agggcttcgg caagggcgtc atctcgccgc ttcccgatcg atcgaactgc atgaagacga
     3181 ggttccatat ttcgacgtaa cgatcgccct cttcgtctgg tgaccccggc gggcctcccg
     3241 gaacatccgg cccgtggtcg tagaagattt cgctgcatgg cccgcaggga ccggtatcgc
     3301 ccatggccca gaaattgtct tttgcgccga ggcgcgagaa gcgctgcggg tcgatgccta
     3361 cctcgttgag ccagatggcg gcggcttcat catcatcctc gaataccgtt atccacagtc
     3421 tctccggcgg cagcccgagg tccttcgtca ggaactccca cgcgaaggca atcgcctggc
     3481 tcttgaaata gtcaccgaag ctgaagtttc cgagcatctc gaaaaacgtg tgatgccggg
     3541 ccgtgtaacc gacgttctcg agatcgttgt gcttgcctcc ggcgcgcacg cagcgctggg
     3601 ccgtgacggc gcggtcgtag cgtcgcttgt cctggcccag gaagacgtcc ttgaactgca
     3661 ccatgccggc attggtgaac agcaacgtcg ggtcattgcc cggcaccagc ggagagctgg
     3721 ggacgacttc atggccgcgg ctcctgaaga actcgaggaa cgcgaggcgc aattcgttgc
     3781 tggtcatgaa cttcatgtga ggtgggggct tatcttggtt ccggctgcgg gatgtagttt
     3841 aacctagcag gtatggccaa cgtgacccc
//
LOCUS       DASUOM010000259         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1570835,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000259 DASUOM010000000
VERSION     DASUOM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570835"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..853)
                     /locus_tag="VFY27_05395"
     CDS             complement(107..853)
                     /locus_tag="VFY27_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5764981.1"
                     /translation="MTQQSLKTNFADIESRRSRSSLRRMSNPRRLYYFLGLPVLRAAL
                     RLLWWTYRVEKVIGADIADRILAEGKACGPCYWHQHHILCTIMIREWIRRGFKACFLV
                     SASVDGDVPARIARAWGAEVIRGSANQTGALVLRDMHQAMKRNVSIVTTADGPSGPQY
                     QFKPGAVLMARIASVPLVPMACAADRAWYLKRWDAFMIPKPFARIVLAVGEPVHVPQD
                     APLDRIDEHRATMQNAVMVLMEQSKRELAK"
     gene            1064..>2086
                     /locus_tag="VFY27_05400"
     CDS             1064..>2086
                     /locus_tag="VFY27_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5764982.1"
                     /translation="MTLAIAANRRPPGPDEPVALGIDPQTLEILLEARQRFGNVVALR
                     STNGRPALFINDPAEIRRILVRQHARYRKGPGFERVKMLLGNGLIVSDGDVWRRSRTM
                     IQPAFSRANMHRLVEVIVTCCKERERRWSAVAEAGGAINMTLETSDFALELILRSMFG
                     EDYERYLLNSGTNPFAFLSEDSTRDLRVVLKVRELRELLLQIVEARRASGGAAKFDFL
                     SIYLAARDKEGNPFEDRELLDELVTLIVAGYETSAGTLNWAWYLLACHPGCLDILVED
                     ARGHLPDAEIDAESVAAMAYTQALLEETLRLYPPVWLFTRRATEDDSLTGFDIAAGTD
                     IFLSPYI"
BASE COUNT          403 a          648 c          639 g          396 t
ORIGIN      
        1 gtcggcgtac gcgggcgcgc cgggccgcaa cgtggtcgcc gcgagcaata acattgcggc
       61 tgccctggtc atccgtggtg atctgagcac tttaagacgg ctcccgttac tttgcgagtt
      121 ccctctttga ctgttccatc aggaccatta ctgcattctg catggttgcg cggtgctcgt
      181 cgatcctgtc caggggcgcg tcctgcggca cgtgaaccgg ctcgcccacg gccagcacga
      241 tgcgcgcaaa gggtttcggg atcatgaagg cgtcccaacg cttcagatac cacgccctgt
      301 ctgcggcaca tgccataggg accagcggca ccgatgcaat ccttgccatc aacacggcgc
      361 ccggtttgaa ctggtattgc ggaccggacg gtccgtcggc cgtcgtgact atcgacacgt
      421 tcctcttcat ggcctggtgc atgtcgcgaa gcacgagcgc gccggtctgg tttgccgaac
      481 cgcgtatgac ttccgcgccc caggcccggg cgattcgcgc cggcacgtcg ccatcgaccg
      541 acgccgagac gagaaagcag gctttgaatc cgcggcgaat ccactcacga atcatgatag
      601 tgcacagaat gtgatgctgg tgccagtagc aggggccgca tgccttgccc tctgccagga
      661 tccggtcggc gatatccgca ccgatcacct tctcgacgcg atacgtccac cacagcagcc
      721 gcaacgccgc cctgagcacc ggaaggccaa gaaagtagta gaggcgccgc gggttcgaca
      781 tacgccgcaa ggaagacctg ctccggcgcg actcgatgtc cgcaaaattc gttttcaggc
      841 tctgctgagt cattggccaa ctgtaaggca cgcgtgccgc gtaaacggtg tgccggagca
      901 caaactgtaa agatgccggg aagcgccttg acctgccatg aatcgcgctg ttcccgggtc
      961 gacggcgtgg gagtttgcgg tatcgaacgg cggccggtca atgtactatt gcgcctgcaa
     1021 aacccggtcc gccccaacat tcaagcccga aactccgtat tcgatgactt tagcgattgc
     1081 agccaaccgg cgtccgcccg gacccgacga gccggtagcg ctgggcatag accctcaaac
     1141 gctcgaaatt ctgctggagg cgcgacagcg cttcggtaac gttgtggcgc tgcgtagcac
     1201 caacgggcgg cccgcgttgt tcatcaacga ccccgcggaa atccgcagga ttctcgttcg
     1261 gcagcacgcc aggtaccgca agggacccgg cttcgagcgg gtcaaaatgc tgctggggaa
     1321 cgggctcatc gtcagcgacg gagacgtctg gcgtcgatcg cgcacgatga tccagccggc
     1381 gttcagccgc gcgaacatgc accgcctggt cgaggtgatc gtgacctgct gcaaggaacg
     1441 cgaacgccgc tggtctgcgg tagcggaagc gggcggcgcc atcaacatga cgctggagac
     1501 cagcgatttc gccctggagc tgatacttcg ttcgatgttc ggcgaggact atgagcgcta
     1561 cctgctgaac agcggcacga atcctttcgc ctttctcagc gaggactcca cgcgcgattt
     1621 acgagtggta ctgaaagtac gcgagctgcg cgaactcctg ctgcagatcg tcgaggcgag
     1681 gcgagcatct ggtggtgcgg ctaaattcga cttcctctcg atttacctcg cggcccggga
     1741 caaggaaggc aacccgttcg aagaccggga gcttctggat gagctggtga cccttattgt
     1801 cgccggctac gaaacctccg cgggaacgct caactgggcg tggtacctgc ttgcctgcca
     1861 ccccggctgc cttgacatac tggtggagga tgcacgcggg catctgcccg atgccgagat
     1921 cgatgcagaa agcgtggcgg cgatggctta cacgcaagcc ctgctcgagg aaacactgag
     1981 gctttatccg ccggtatggc tgtttactcg acgtgccacg gaggatgatt cgctcaccgg
     2041 tttcgacata gcagcgggca ccgatatctt cctgtcgccg tatatt
//
LOCUS       DASUOM010000260         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_933880,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000260 DASUOM010000000
VERSION     DASUOM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933880"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1012
                     /locus_tag="VFY27_05405"
     CDS             23..1012
                     /locus_tag="VFY27_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5764983.1"
                     /translation="MQRGDVEILELRKPGGKHPPTYWRIFTELRRLRPDIVHTRNLGT
                     LDLAWIARVAGCPIRIHGEHGWSADDPDGSSVKYRFLRKICDPAIDRYVAVSENITHW
                     LVDEIGIPKHRVTTLHNGVDSERFRKTDSGAAGASVDSPIVIGTVGRQEPIKGLEVLL
                     MALRDLLVLRPELRSRLQVLMGGDGPSHQNCLDLRDRFGLNDIVEFPGRVIDVPAYLR
                     QLTIFIQPSLNEGISNTVLEAMASGLPIIATNVGGNPELIRDGKDGRLVPPNDTSALR
                     MAIEKYLDDAAARERDGTEARARVERCFSLDTMVGKYAQLYNDAVAEATRRAN"
     gene            1169..3112
                     /locus_tag="VFY27_05410"
     CDS             1169..3112
                     /locus_tag="VFY27_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEX5764984.1"
                     /translation="MCGIAGIYDVQGHRDVDRQVLKRMTDVLSHRGPDGSGYHSEPGV
                     GLGHRRLAIIDLEGGKQPLYNEDDTVAVTYNGEIYNFQPLQKELERLGHRFRTRCDTE
                     VIVHAWEEWGEDCVTRFNGMFSFGIWDRNRDCLFLARDRIGIKPLYYGTTRDGFLVFA
                     SELKGLLEHPALKRELRPDAVEDYFAFGYIPDPKTILHSAWQLSPGHTLTVRRGQSLS
                     PRQYWNVRFGDAVARKDGELYEELRARLRQSVERRMIADVPLGAFLSGGIDSSAVVAC
                     MAQLSSGPVVTTSISFGDAEYNESRYAQMVADRYQTSHRVKQVDPSDFSLIDELPALY
                     DEPFADSSALPTYRVCELARQNVTVALSGDGGDEDFIGYRRYRWHAYEEMVRRRLPQS
                     VRGPLFGAAGILYPKMDRAPKVLRAKSTLQALARDSLEGYFHSVTILPASARERLFGA
                     AFRRELQGYTAIEVFRKHCANAPVRDGMELIQYLDYKTYLPGDILTKVDRASMAHSLE
                     VRVPLLDHEFVTWVATIPSSAKLNGKEGKVCFKRAMEPHLPRDVLYRPKMGFGVPISS
                     WFRGPLKDVVRNRLLNGRLNDMGIFDETYIEQLVNEHQSARREHSAVLWALLMFEGSM
                     SRVFDRAPGVDRQPSSAVSAEYA"
     gene            3112..>3988
                     /locus_tag="VFY27_05415"
     CDS             3112..>3988
                     /locus_tag="VFY27_05415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5764985.1"
                     /translation="MRILYHHRIASKDGQFVHVEELVKALRRAGHDVRIVGPTYVESG
                     RFGSERKYIHRLKRMLPRFAYEILELAYAIPAYFRLIRAARQYKPDVIYERYNLFQPA
                     GIWLRNRTKVPLLLEVNAPLFEERSVFSGISLKAIASWSERYVWRNADAVLPVTKVLA
                     EYLLAAGVEKSKVTVIPNGVDLAEFKGGMDRSAAKRRLHVDGKLVIGFVGFVREWHRL
                     DTIIRLLADADFANVVLLVVGDGPARAQLESLATSIGVVERVQFAGLVERENLLSCMS
                     AFDIALQPSVVRYASP"
BASE COUNT          801 a         1136 c         1264 g          787 t
ORIGIN      
        1 tgcaacggat ttccgccagc gcattcagcg gggagatgtc gaaatcctgg agctgcgcaa
       61 gcccggaggc aaacatccgc ccacctattg gcgtatcttt acggagttgc ggcggctgcg
      121 gccggatatc gtgcacacac ggaacctcgg gacgttggat ttggcctgga tcgctcgtgt
      181 ggccggatgt ccgatcagga tccatggtga gcacggttgg tcggccgatg accccgatgg
      241 atcgtccgtc aaataccggt ttctaaggaa gatctgtgat cctgcaattg acaggtatgt
      301 ggcggtgtcc gaaaacataa cgcactggct ggtcgatgag attggtatcc caaagcaccg
      361 ggttacaacc ttacataatg gggtagattc ggagcggttc cggaagacag acagcggcgc
      421 agccggggcc agtgtcgact cacctattgt gattggcacc gtcggccgtc aggagccgat
      481 caagggcctg gaggtcttgt tgatggcgct gagggacctt ctcgtgttga ggccggagtt
      541 gcgatctcgc cttcaggtac ttatgggtgg agacggaccg agtcaccaga actgcctgga
      601 cctccgggat cgttttgggc tgaatgacat agtggaattc ccgggtcgag tcattgacgt
      661 gccagcctat ttgcgccaac tcactatctt catacagcca tcgttgaatg agggaatctc
      721 gaatactgtg ctggaggcaa tggccagcgg attgccgatc atcgcgacaa atgtcggcgg
      781 taatcccgaa ctgatccgcg acggcaagga tggccgtctg gtccccccga acgacacgtc
      841 tgctttgcgt atggcgatcg aaaagtatct ggacgacgct gctgcgcgcg aacgcgatgg
      901 cacagaagcg cgggcacgcg tcgagcggtg cttttctctc gacacgatgg tgggaaaata
      961 tgcgcagctt tacaacgacg cagttgccga ggctacgcgg cgcgcgaatt gagaaaagtc
     1021 gatgtgtgag ataactggca ttttcgctgt cgaggctcga cgagaaatag acccggcact
     1081 actcgaaaga atgaatagtg cccactaccg gaatctgtat gatcaatgcc tttacaaccg
     1141 gcatcgagta acaacggggc acacgtgaat gtgcggtatc gcgggaattt atgacgtcca
     1201 gggtcatcga gacgtcgatc ggcaagtgct gaaacgaatg acggacgtcc tgagccaccg
     1261 cggcccggac ggcagcggct atcactcgga acccggcgtc ggcctcggcc accgtcgtct
     1321 cgccatcatc gatctcgaag gtggcaagca gccgctctac aacgaggacg ataccgtggc
     1381 cgttacgtac aacggcgaga tttacaactt ccagccgctg cagaaggaac tggagcgcct
     1441 gggacaccgt ttccggacgc gctgcgacac cgaggtcatc gtccacgcct gggaagagtg
     1501 gggcgaggat tgcgtcacgc gtttcaacgg catgttttcg ttcgggatct gggaccgcaa
     1561 ccgggattgc ctgttcctcg cacgcgaccg catcggcatc aagccgctgt actacggcac
     1621 gacgcgcgac ggcttcctgg tgttcgcctc ggagttgaaa ggcctgcttg agcatccggc
     1681 gctcaagcgg gagctgcgcc cggacgcggt cgaggactat ttcgccttcg gctacatacc
     1741 cgatcccaag acgatcctgc atagcgcgtg gcagctctcc cccgggcaca cgctgacggt
     1801 caggcgcggg caatcgctct cgccacgcca gtactggaac gtccgctttg gcgatgccgt
     1861 ggcgcgcaag gacggggagc tgtacgagga gctgagagcg cggctcaggc agagcgtcga
     1921 gcgccgcatg attgctgacg tgccgctcgg cgcgttcctc tcgggcggca tcgattcgag
     1981 cgccgtggtc gcctgcatgg cgcagctctc cagcggcccg gtcgtcacga cgtcgatctc
     2041 cttcggcgac gcggagtaca acgagtcccg ttacgcgcag atggtggcgg atcgctacca
     2101 gaccagccat cgcgtcaagc aggtggatcc ttccgatttc agcctgatcg acgagctgcc
     2161 ggcgctgtac gacgaaccgt tcgccgacag ctccgcgctg ccgacttacc gcgtctgtga
     2221 gctcgcgcgg cagaacgtca ccgtcgcgct ctccggcgac ggcggcgatg aggatttcat
     2281 cggctatcgc aggtatcgct ggcacgcgta cgaggaaatg gtgcggcgcc gcctgccgca
     2341 gagcgtccgt gggccgctgt tcggtgcggc cggcatcctg tatccgaaga tggaccgggc
     2401 acccaaggtc ctgcgcgcca agtccacgct gcaggcgctc gcaagggatt cactggaggg
     2461 ttattttcac agcgtcacga tcctgcccgc gagcgccagg gagcgcttgt tcggtgcggc
     2521 attccgccgc gagctgcagg ggtacacggc gatcgaagtc tttcgcaagc attgtgcgaa
     2581 tgcaccggtc agggatggca tggagctgat ccagtacctc gattacaaga cctacctgcc
     2641 gggcgacatt ctgaccaagg tcgaccgggc cagcatggcg cacagcctgg aagtccgggt
     2701 gccgctcctc gatcacgagt tcgtcacgtg ggtggcgaca atcccgtcgt ccgcgaaact
     2761 gaacggcaag gaaggcaagg tgtgtttcaa gagagccatg gaaccgcacc tgccgcgcga
     2821 cgtgctctat cggcccaaga tgggctttgg cgtgccgatc agcagctggt tccgcggtcc
     2881 gctgaaggac gtggtaagga accggcttct caacggtcgg ctcaatgaca tgggcatctt
     2941 cgacgagacc tacatagagc agctcgtcaa cgagcatcag tcggcacgac gggaacacag
     3001 cgcggtgctg tgggcgctgc tgatgttcga gggtagcatg tcgcgcgttt tcgatcgtgc
     3061 cccgggcgtc gaccggcaac catcgtctgc ggtttcagcg gaatacgctt gatgcgcata
     3121 ctctatcacc acaggatcgc atcgaaagac ggacagtttg tacacgttga ggagctagta
     3181 aaggcacttc ggcgtgcggg gcacgacgtg cgcatcgtcg gcccgaccta cgtagaaagc
     3241 ggccggttcg gctccgagcg caagtacatt caccgactca aacgcatgct accgaggttc
     3301 gcctatgaga tcctcgagct ggcatacgcg attccggcgt acttccggct catccgagcg
     3361 gcgcgtcagt acaagccgga cgtcatctac gagcgctaca atctctttca gccggccggg
     3421 atatggctcc gcaaccggac gaaagtaccg ttgctactcg aagtcaacgc gcccttgttc
     3481 gaagaacgat ccgtctttag cggaataagc ctcaaggcaa tagcgtcctg gagtgaacgg
     3541 tacgtctgga ggaacgctga cgccgtacta ccggtaacca aagttcttgc cgagtatctg
     3601 ctcgcagcgg gagttgagaa gtcgaaggtg accgtaattc ctaacggagt cgatcttgcc
     3661 gagtttaagg gtggaatgga tcgcagcgcc gcaaagcgcc gattgcatgt tgacggcaag
     3721 ctcgtcatag ggtttgtcgg gtttgttaga gaatggcaca gactggacac gattatccgg
     3781 ctgcttgcag atgcggactt cgcgaatgtc gtcctgctgg tggttggtga tggacccgcg
     3841 agggctcagc tggagtcgtt ggcgacgagc atcggtgtcg tagagagggt tcagtttgcc
     3901 ggcctcgtgg agcgagagaa tctgctgagt tgtatgtcgg ctttcgacat cgcgttacaa
     3961 cccagcgtcg ttcgatatgc gtctcctc
//
LOCUS       DASUOM010000261         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1246244,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000261 DASUOM010000000
VERSION     DASUOM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246244"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFY27_05420"
     CDS             <1..248
                     /locus_tag="VFY27_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5764986.1"
                     /translation="ARFSFLLSAPVILLATLYEVTILLIDGGAVSWSTLGVGALVSGL
                     VAYLSIEFFMRFVTTIGLLPFAIYRLVLAAAIFLLLL"
     gene            complement(273..1529)
                     /locus_tag="VFY27_05425"
     CDS             complement(273..1529)
                     /locus_tag="VFY27_05425"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX5764987.1"
                     /translation="MEVYLVGGAVRDELLGLPVTERDWCVVGATPGELEAAGYRQVGK
                     DFPVFLHPRTGEEYALARTERKTGPGYHGFSFHSSPDVTIEDDLRRRDLTVNAMARDS
                     SGILVDPFGGRKDLEQRVLRHVSDSFAEDPVRILRVAKFAARFAPLKFRLAEETAALM
                     AAMVENGEADELVPHRVWKECEAALACARPSVFFDILRQCGALAVVFPEIDALFGVPQ
                     PEQWHPEIDCGIHAMMVLEQAAALSREVDVRFAALVHDLGKATTPKNKLPSHPGHERR
                     SVRLIKSMASRMPIPTACRDLALVVAEFHGHCHKAFELRPSTILKVLERTDAFRRRER
                     FDKFLLACEADSRGRTGFEQRPYPQAAWFRQALDAAAEVSARDVLREGMEGAQIGDAL
                     RRKRLQALSRLKSDYQAEASSQESAR"
     gene            1762..2802
                     /locus_tag="VFY27_05430"
     CDS             1762..2802
                     /locus_tag="VFY27_05430"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5764988.1"
                     /translation="MKIIAGALALAALFTLEGCYYMQAVRGHMDVMQRRRPVPEVLDD
                     PDAPQNLKERLELVQEAREFSVEELELPDNDSYRSYADLGREFVVWNVLAAPEFSLRA
                     KEWCFPVAGCVAYRGYFAEQAAQREAESLSDSGYDVSIGGVSAYSTLGRFSDPVLNTM
                     MRWSDTDLIATLFHELAHQKVYVKNDTQFNESFATAVAEIGVERWFAARKEPQLLNVY
                     LERKASRRELLQLVEKAKIQLAVLYDSGLEEAEMRQRKDAILNRLSQDVSSLLSRNGA
                     GAASWPVGALNNARLVSLGLYEGWLPAFRELYKNCNGKLTCFYATVGSIADLPQDERH
                     ARLIVLTAEAAG"
     gene            complement(2891..4870)
                     /locus_tag="VFY27_05435"
     CDS             complement(2891..4870)
                     /locus_tag="VFY27_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5764989.1"
                     /translation="MRPLSRTCLALMVASIACAAPAATIDEQRAAFREVYPAAERGEW
                     EPIEEKRPVLEDYVLWPDLKAAWLRSHLDDAREVEQFLEQYGSLKAARELRYRLALRL
                     ANEKRFPEYLEIYRRWYANLEIAKLDCLAVHADILAGSDASVASRGRDLWLIGRSQAE
                     ECDPVFEYLRGAGVLDEQLYRDRFELAITERQFSLARYLARSLDQRHLEQANRWIAAQ
                     NGAGEFLQSAHADRADDELYREQLTYALERLSYSDAELAAEHWRILRRAYAFPADEAR
                     GIARHIALWLARQHNPEGFQALRDLPPEAVDVEVRRWLVRSALRQHSWPDVVSAIAGM
                     PGDERTKEEWQYWLAVAWKETGNVDEARTIFEAIAGERSYFGFLAADEIGSDYAWAHT
                     SLQEDTGSLKTLAANAALVRARELFYVGLESRGRSEWDDAVSMLPPGEKAQAALLAHR
                     WGWHSRAIATAASVSLYDDLELRYPLPYREQFERHSRTAAIPDSWAYGVARSESLFMA
                     DIRSIAGAVGVMQLMPSTGRRTASEIKLPYNGIDTLTDPDSNIRLGTFYLGKMLQRFG
                     DNAVLATAAYNAGPARVEQWLPDEDQLDARIWIENIPFNETRGYVRRVLATDAIFHWR
                     LTGKTRRLSHHLAAIDPASRTQQSADAARRTMGGP"
BASE COUNT          937 a         1556 c         1573 g          983 t
ORIGIN      
        1 cggcaaggtt ttcatttctg ctttctgcac cggtcattct gcttgcgacg ttgtacgagg
       61 tgacgatcct cctcatcgac gggggcgccg tctcctggag cacgcttgga gtgggagcgc
      121 ttgtttcggg gctggttgcc tacctcagca tcgagttctt catgcgtttc gtcaccacga
      181 tcggcttgct gccgttcgcg atctaccgct tggtactcgc cgccgccatc ttccttctcc
      241 tgctgtaggg ggccgcgcgg caggatttca tgctaccgcg cagactcctg gctcgaagcc
      301 tcggcctggt agtcgctctt gaggcggctt aaagcctgca gccgcttgcg gcgaagcgca
      361 tcgccgatct gcgcgccctc cattccctcg cgaagcacgt cgcgtgcgga tacttcggcg
      421 gccgcgtcca gcgcttgcct gaaccaggcc gcctgtggat aaggccgctg ctcgaagccg
      481 gtcctgccgc gcgaatccgc ttcgcacgcg agcaggaact tgtcgaaccg ttcccggcga
      541 cggaatgcat cagtccgctc aagaaccttg aggatcgtcg acgggcgcag ttcgaaagcc
      601 ttgtggcaat gaccgtggaa ctctgcgacg acgagagcga ggtcgcggca agccgtcggt
      661 atgggcatgc gcgacgccat tgacttgatc agcctgacgc tgcgtctttc gtgtcccggg
      721 tgacttggca acttgttctt tggggtcgtg gcctttccca ggtcatgcac cagcgcggca
      781 aaccggacat cgacctcgcg gctcagcgcg gcagcctgtt cgagcaccat catcgcgtgt
      841 atgccacagt caatttccgg atgccactgt tccggttggg gcacgccgaa cagcgcgtcg
      901 atttccggga atacgacagc cagggcaccg cattgccgca ggatgtcgaa gaagaccgaa
      961 ggccgggcgc aggcgagggc ggcttcgcat tccttccata cccggtgcgg cacgagctcg
     1021 tcggcttcgc cgttttcgac cattgccgcc atcaatgccg ccgtttcttc tgccaggcgg
     1081 aacttaagcg gcgcgaacct ggcggcaaac ttcgccactc tcaggatgcg tacaggatcc
     1141 tcggcaaagg aatccgagac gtggcgaagc acacgctgct ccagatcctt ccgcccaccg
     1201 aaggggtcga cgaggatgcc gctcgagtcc ctcgccattg cattgaccgt caggtccctg
     1261 cgccgcaaat catcctcgat ggtgacatcc ggtgacgagt ggaacgagaa gccgtgatac
     1321 ccgggccctg tcttgcgctc cgtgcgggca agcgcgtact cctcacccgt tcgcgggtga
     1381 aggaacaccg gaaaatcctt gccgacttgc ctgtagcctg cagcctccag ttccccgggg
     1441 gtggcgccga cgacgcacca gtcgcgctca gtcaccggca gcccgagcaa ctcatcgcga
     1501 actgcgccac ccaccagata gacttccatc ggcgaattct agtgtcgtgt cccgcaaatg
     1561 actacgctat tccggttgtg tcgaaccttt gacgagcaga aggctcttac cagatcgtca
     1621 ctgcgcgagt cgcttaagaa tcgtaatgtg acatgtaacg atgttttcgc cccgtcactc
     1681 acggaatatg ccgattcccg gcattggcaa aaggttaaag ctcgtcgaat gcaggaaaag
     1741 aaattgccat cccggcgccg cgtgaaaata atcgcaggcg ctctcgccct cgcagcgctc
     1801 ttcaccctgg aaggctgcta ttacatgcag gccgtccgtg ggcacatgga tgtcatgcag
     1861 cgcaggcgtc cggtaccgga ggtgctggat gacccggatg cgccgcagaa cctgaaggag
     1921 cgcctcgaac tggtacaaga agcgcgggaa ttttctgtcg aggaactcga gcttccggac
     1981 aacgacagct accggagcta cgcggacctc gggcgggagt tcgtcgtatg gaacgtcctg
     2041 gccgcgccgg agttttcgct gcgggccaag gagtggtgct tccctgtcgc cggttgcgta
     2101 gcctatcgcg ggtattttgc ggaacaggcc gcgcagcggg aagcggaaag tctgagcgac
     2161 agcggctatg acgtctctat cggcggtgtc tccgcctatt cgaccctggg gcgattttcc
     2221 gaccctgtgc tgaatacgat gatgcgctgg agcgataccg acctgatcgc gaccctgttc
     2281 catgaactcg cgcaccagaa ggtgtacgtg aagaacgaca cgcaattcaa cgaatccttc
     2341 gccacggctg tcgcggagat cggggtggag cgatggtttg cagcccggaa ggagccgcag
     2401 ttgctgaacg tttatctcga gcggaaagca tcgcggcggg agctactgca actggtggag
     2461 aaagcaaaga ttcagctcgc agttctttac gattcaggac tcgaagaagc ggagatgcgg
     2521 cagcggaagg atgcgatcct caacaggctg tcgcaggacg tcagcagcct gttatcgcga
     2581 aacggcgcag gtgcggcgag ctggccagtg ggtgctctca acaatgcaag gctggtgtca
     2641 ctggggcttt acgaggggtg gctgccggcg ttccgtgaat tgtacaaaaa ctgtaacggc
     2701 aagctcacct gcttttatgc aacagtcggc agcattgccg atcttccgca ggatgagcgc
     2761 cacgcccgac tgatcgtgct aacggcagag gctgccggct agggaagagc gctgcaggag
     2821 ccggccaaac cgcgggcgag tgcagtgtgc gccggcgaac tcgcccatgc ggtgggctgc
     2881 tgcggttcgt ctacggaccg cccatcgtcc gccgcgcggc atcagctgac tgctgcgtgc
     2941 gggacgcggg atcaattgcg gcaagatgat gggagagccg ccgcgtcttt cctgtcagac
     3001 gccagtgaaa gatcgcgtcg gtggcgagca cgcggcgcac atagccgcgc gtttcgttga
     3061 acgggatatt ctcgatccag atcctagcat cgagctgatc ctcgtccggc agccactgtt
     3121 ccacccgtgc gggcccggca ttgtaggcgg ccgtcgccag caccgcgttg tcgccgaacc
     3181 gctgcaacat ttttcccagg taaaaagtcc cgaggcggat gttgctgtcc ggatcggtga
     3241 gggtgtcgat gccgttgtac ggcagcttta tttctgaagc ggtgcggcgc cccgttgacg
     3301 gcatgagttg catcacaccg acagcgcctg cgattgaacg gatatcggcc atgaaaagac
     3361 tctcgctgcg cgctacgcca taagcccagc tgtccgggat ggccgccgtt cgggaatgtc
     3421 tctcgaactg ctcgcgatac ggcagcggat agcgcagctc gaggtcgtca tagaggctca
     3481 cggaagcggc cgttgctata gcccttgaat gccagcccca gcgatgcgcg agaagtgccg
     3541 cctgtgcctt ttccccgggc ggaagcatgg aaacggcgtc gtcccactcg gacctgccgc
     3601 ggctttcgag cccgacataa aagagctccc ttgcccgtac cagcgctgcg tttgctgcaa
     3661 gcgttttgag cgacccggtg tcttcctgca gcgacgtgtg tgcccaagcg tagtcgctgc
     3721 cgatttcatc tgctgcgaga aatccgaaat agcttcgttc gccggcaatt gcctcgaata
     3781 tggtgcgtgc ttcgtcaacg ttacccgtct ctttccacgc gaccgccagc cagtattgcc
     3841 actcttcctt agtccgttcg tcgccgggca ttcccgcgat cgcggagacg acatcgggcc
     3901 atgagtgctg ccgcagcgcc gagcgcacca gccagcgccg cacttcgacg tccacggcct
     3961 ccggcggcag gtcccgcagg gcctggaatc cctcggggtt atgctggcgc gccagccaga
     4021 gcgcgatgtg gcgggcgatt ccccgggctt catcggctgg aaaggcgtaa gccctgcgca
     4081 ggatccgcca gtgttccgcc gccagttcgg catcgctgta agagagccgc tcgagagcgt
     4141 aggtcagctg ttcgcggtac aactcgtcat cggcgcggtc cgcatgcgcg gactgcaaga
     4201 actcgccggc gccgttctgg gcggcgatcc agcggtttgc ctgctcgagg tgccgctgat
     4261 ccagggagcg ggcgaggtat cgtgcgagcg aaaactggcg ttcggttatc gccagttcaa
     4321 agcggtctcg gtaaagctgc tcgtcgagga ctcccgcgcc tcgcaagtat tcaaaaaccg
     4381 gatcgcactc ttctgcctgg ctgcgcccga tgagccacag gtcgcggccg cggctcgcca
     4441 cgctagcgtc gctgccggcc aggatatcgg catgcacggc gagacagtcg agcttcgcga
     4501 tctcgaggtt cgcataccag cggcgataaa tttcaaggta ttcggggaaa cgcttctcgt
     4561 tcgcgaggcg cagcgccagg cggtaacgaa gttcccgcgc ggccttcagg ctgccgtact
     4621 gttcgaggaa ttgctcaact tccctcgcat cgtcgagatg agagcgaagc cacgcagctt
     4681 tcaggtcagg ccacagtacg tagtcctcga gaaccgggcg cttctcctct atgggttccc
     4741 attcacctcg ttcagcggcc ggatagactt cgcggaatgc cgcgcgctgc tcatcgatgg
     4801 tggcggcggg cgcagcgcaa gcgatactgg caaccatcaa tgcgaggcag gtgcgggata
     4861 gtggtcgcat gcgtgctcac tgctgaaggc ctaagttgtc gcgacgatac tggcgacaca
     4921 ggccacctgc aagtcggaac caaagtccga tgagcctgca aaaaagcccg gcacgaagac
     4981 cgggcgtccg catccgtgac gattagcgaa tctgcagcat caactgccgg tcggcccggc
     5041 ggacgagca
//
LOCUS       DASUOM010000262         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_41578,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000262 DASUOM010000000
VERSION     DASUOM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41578"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFY27_05440"
     CDS             complement(<1..878)
                     /locus_tag="VFY27_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5764990.1"
                     /translation="MGSTPRVVVVGGGFGGLSAARALGSAGVRILLIDKQNHHLFQPL
                     LYQVATAALSPADIAWPIRRILRRQQNAEVLMAEVTGVDPLARRVLLGHRSEAYDYLV
                     LASGATHSYFGHDEWAPYAPGLKEIVDATQIRRRILMAFECAEAVNDSGRRQKFLTFV
                     VVGGGPTGVEMAGTMSELARKALAADFRRIDLRTARVVLVEAGPRLLSSFPESLSDYA
                     RQALVDLGVDVQTGVAVTGCDADGVDTASGRIDAATIVWAAGVEASPVARWLDVPADR
                     AGRVIVGEDLSVPGRDG"
     gene            1042..1536
                     /locus_tag="VFY27_05445"
     CDS             1042..1536
                     /locus_tag="VFY27_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5764991.1"
                     /translation="MFKKTQSEENQAPAPAQPAAADPVMVRTRSVSVIGPTLVFKGEL
                     SADEDLVIQGTIEGTIAHHKKNLTVGKEGRVKADINAASVTIEGHVEGDIHGDDFVEL
                     AKSAVVTGNIFCGRIKMADGATFNGSIEMGAPRASQAKLSVAEDRGGRDASRDPARTH
                     NIGA"
     gene            1577..1957
                     /locus_tag="VFY27_05450"
     CDS             1577..1957
                     /locus_tag="VFY27_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5764992.1"
                     /translation="MAERGRRAAGGQPSCIGSTMVLKGDLSLEEDLIIEGRFDGTITQ
                     GDHRLSIGEHARVKADIRTGSAIIAGLVDGNVYGSSTVVVKKTARLHGALTAEKLCLE
                     SGANLEDVILSGNIARIRTQSRDR"
     gene            complement(2022..2996)
                     /locus_tag="VFY27_05455"
     CDS             complement(2022..2996)
                     /locus_tag="VFY27_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5764993.1"
                     /translation="MKSLLMTLSSNRSIETGASCKLGMGVILLALGACTTTPERVPEL
                     DQARSAVESLQSEPRAREAASIQLTKAQEALQRAEAAMEDGQPIEQIRHEAYMARRNA
                     EIGLELTSEAEATQEISQAEARRREVQLEARTAEAESASRMAEQRAMQAEQSQREAQA
                     SQAVAEAAIEEANRLADELNAMEGELEAERTERGLVLTLGDVLFDTNGDELKAGADVS
                     INRLAAFLNENPERRLLIEGHTDARGAEEYNKDLSERRADAVAEALVERGIPSEKLRS
                     VGLGEEYPVASNDTSAGMQQNRRVEIVVSNADGSFPAPAEQRALAARD"
     gene            complement(2993..3388)
                     /locus_tag="VFY27_05460"
     CDS             complement(2993..3388)
                     /locus_tag="VFY27_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX5764994.1"
                     /translation="MNNTWTKPRTLLLAVPLASLCACASSPDRPFQDLARAEASIEQA
                     DRSGAQEYGAIELDAARDKLAKARAAADSGENIAASRYAKEAAVDAELATAITRNREA
                     ELAVEELNRSIETLREEIARNQARSGDRQ"
BASE COUNT          646 a         1220 c         1062 g          730 t
ORIGIN      
        1 ccgtcgcgtc ccggcaccga aagatcctcg ccgacgatca ccctccccgc ccggtccgcc
       61 ggcacgtcca gccagcgggc cacgggcgaa gcctcgacac cggctgccca tactattgtt
      121 gcagcgtcaa tgcgtcctga agccgtgtcc actccgtcgg catcacagcc tgtgaccgca
      181 acgccagtct gcacgtcgac cccgagatcc accagcgcct ggcgcgcata gtcgctcaag
      241 ctttcgggaa atgaggacag gaggcgcggg ccggcctcca ccaaaaccac gcgcgcggtg
      301 cgcagatcga tacgcctgaa atccgccgca agcgccttgc gtgcgagttc cgacatcgtt
      361 cctgccatct cgacgccggt cggcccaccg ccgactacaa cgaacgtaag aaatttttgc
      421 cgacgcccgg aatcatttac agcctccgcg cattcgaaag ccatgagaat cctgcggcgg
      481 atctgggttg cgtccactat ctccttgaga cccggggcgt acggcgccca ctcgtcgtga
      541 ccaaaatagg aatgagtagc cccgctcgcg aggacgaggt agtcatacgc ctccgaccgg
      601 tgtccgagaa ggactcgcct ggccagcgga tcgacccccg taacctccgc catgagtacc
      661 tccgcgttct gctgccggcg aagaatgcgc cggatcggcc aggcgatatc cgcgggcgac
      721 agcgcggcag tcgctacctg gtacagaagt ggctgaaaga gatgatgatt ttgcttatcg
      781 atcagcaaga tgcggacgcc ggcacttccg agcgcccggg ccgcgctcag accgccgaac
      841 ccgccgccga cgacgacaac ccgcggagtg gaacccattt tcctccctgg cgccggaatg
      901 gcgcatagcc cggatctgga gcaccgattt gcgctactct atcggcgcaa tcaacttctt
      961 cggacacctt cagtctacgc gcacgatggg ctccacgccc ggtgtcccga agcaatccgt
     1021 gcagatctga aggagacacc gatgttcaag aagacccagt ccgaggagaa ccaggctccc
     1081 gcaccggctc aacccgccgc cgccgacccg gtcatggttc gtacacgcag cgtttccgtc
     1141 atcggtccca ccctcgtgtt caaaggcgag ctctcggcgg acgaggatct cgtgatccag
     1201 ggaacgatcg agggcaccat cgcgcaccat aagaagaacc tgaccgttgg gaaggaaggg
     1261 cgcgtgaagg cggatatcaa cgcggcctcc gtgacgatcg aagggcacgt ggagggcgac
     1321 atccacggcg atgacttcgt cgaacttgcc aaaagcgccg tggtgaccgg aaacatcttt
     1381 tgcgggcgca tcaagatggc cgatggggca acgtttaacg gcagcatcga gatgggcgct
     1441 ccccgggcga gccaggcgaa gctgtcggta gcagaagacc gcggcgggag ggacgcctcc
     1501 cgggacccgg ccaggacaca taatattggg gcgtaacccg ctccgaatgt cgcaagtgca
     1561 ccccatgcct gacgagatgg cagaacgagg ccgtcgggcc gctggcggcc agccttcgtg
     1621 tatcggctcc acaatggtcc tgaagggcga cctgtcgctc gaggaggacc ttattatcga
     1681 gggtcgcttc gacgggacga ttacccaggg cgatcaccgt ctttcgatcg gcgagcacgc
     1741 cagggtaaag gccgatatac gtacgggctc tgcgattatt gccgggcttg tcgacggaaa
     1801 cgtttacgga agcagcaccg tcgtcgtaaa aaaaaccgcg cgcctccacg gggcattgac
     1861 cgcggaaaaa ctttgcctcg aatccggagc gaacctcgag gacgtaatcc tgtccggaaa
     1921 tattgcccgc atccgcacgc agtcacggga tcgatagccg gtgaccggtg aatgcaggag
     1981 cccgcgaagc gcgggctcct gcaacgcttt tacggcgagt tctagtctcg cgcagccagg
     2041 gcgcgttgct ccgccggagc ggggaacgag ccatccgcat tcgacaccac gatttctacg
     2101 cgccggttct gttgcatgcc ggcggatgta tcgttactcg cgaccggata ttcctcaccc
     2161 aaaccgacgg atcgcagctt ttcgctcggg atgccgcgct ccaccagcgc ttcggccacg
     2221 gcatccgcac gcctctcgct cagatccttg ttgtattctt ccgcaccgcg ggcatcggtg
     2281 tgtccctcga tcagcaggcg acgctccggg ttttcgttca ggaaagctgc cagccgattt
     2341 attgacacat cggcacctgc cttcaactcg tcaccgtttg tgtcaaagag gacatcgcct
     2401 aacgtcagca ccaggccgcg ctcggttcgc tcagcctcga gttcaccttc catcgcgttc
     2461 aactcgtccg ccaatcgatt ggcctcctcg attgccgctt cagcgacggc ttgcgatgcc
     2521 tgtgcttcac gctggctttg ttccgcctgc atggcacgtt gctccgccat cctgctcgcg
     2581 ctttccgctt cggccgtgcg cgcctccagc tgtacttccc gtcgccgcgc ctctgcctgg
     2641 ctgatctctt gggtcgcctc ggcttccgac gtcagttcca ggccgatctc ggcattccgc
     2701 cgcgccatgt atgcctcatg ccggatctgc tcgatcggct gcccgtcctc catggccgcc
     2761 tccgctcttt gcagcgcctc ctgcgctttg gtgagctgga tcgacgccgc ctctcttgcc
     2821 cgaggttcgc tttgcagcga ttccactgcg ctccgcgcct ggtcaagctc gggtacccgt
     2881 tcaggcgttg tggtgcatgc tccgagtgcg agcaggatga ctcccattcc gagcttgcag
     2941 gacgctcccg tctcaattga acggttcgat gatagtgtca tcagtagtga tttcattgcc
     3001 gatctcccga ccgcgcctgg tttcgcgcga tctcctcccg cagggtttcg atgctcctgt
     3061 tgagctcctc cacggcaagc tctgcctcac ggttgcgtgt gatggcagtc gcaagctccg
     3121 cgtccacagc agcttccttc gcgtagcggg acgcagctat gttttcaccg ctgtcggccg
     3181 ctgctcgcgc ctttgcaagc ttgtcgcgcg ccgcgtcgag ttcgatcgcg ccgtactcct
     3241 gagcaccgga tcggtcagct tgttcgatac tcgcctcggc cctcgccagg tcctggaaag
     3301 ggcggtccgg actggatgcg caggcgcaca ggctcgccag gggtaccgca agcagcagcg
     3361 tcctcggctt cgtccacgtg ttgttcatcg cttgaatctc ctgacatgtt tcgcggtcga
     3421 ttagaattct gcggcagtga tccgcagtga ctattgactc acgtgcagta ttcgtgccag
     3481 tagtcttgcg tgcgtctcgc cgctctccct ccgggcgtaa gcatttttgc aagccttttg
     3541 taagctttac cgtcccctgg cgccctgcgc cggttgcagc ccctgtttct ccgcttggcc
     3601 gagttggcac ggcatttgct tgagtactgc cgtaatggcc ggttcggctc taaagaca
//
LOCUS       DASUOM010000263        12389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_716633,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000263 DASUOM010000000
VERSION     DASUOM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716633"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..323
                     /locus_tag="VFY27_05465"
     CDS             69..323
                     /locus_tag="VFY27_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5764995.1"
                     /translation="MRELVLVLVVAAGCTTMPPGEREAMEYKRTDARLKATEEYNALR
                     AGCRAAGGVLVVEGMASRWPRTSLGMKSARCMESRPPVIF"
     gene            507..1907
                     /locus_tag="VFY27_05470"
     CDS             507..1907
                     /locus_tag="VFY27_05470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5764996.1"
                     /translation="MGKFIAVLLLLAAAAGAVWYFAGEADPPVIEIAQPARLVGQTGE
                     LDVAIAAPRGELTRLQVTLQQGTTQLPVFALPSAGSAQLARESEHRMRLTGSIGKRDI
                     PDIVAGPLTIEVTAARPVLFGLREVESTATQEVEVRLTPPHVSVASQFHYINHGGSEM
                     VVYRVRPEDVESGVQVGEYEYPGYPASGAGIAGADPGLRVAFFALLWDQDVDTPINLF
                     ARDEVGNEARARFDYRVFPKKFRSSRINVSDSFLQKVVPEILYNTPELEVPDPDDLLA
                     SYLQINRELRRQNNARIAAMVAETSPGILWRGAFKQLTNTAVEAGFADQRTYVHNGND
                     VDRQVHLGFDLASTTNAPVLAANSGRVVHAGWLGIYGNCVILDHGMGLQSLYAHLSSI
                     RVGEGDEVGMNGQLGLSGETGLAGGDHLHFTMLLAGHAVTPIDWWSQQWVDDRIMRKL
                     RQAAPGQPAPAARAGQ"
     gene            1834..2817
                     /locus_tag="VFY27_05475"
     CDS             1834..2817
                     /locus_tag="VFY27_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5764997.1"
                     /translation="MTASCENYGRRPPANRLLPRGQASRVTGTRVPADTKVAAGAGRL
                     SPLILACLAATWFVWGSTYLAIKFALGSFPPFFQMGSRFLVAGALLMLWVRWRGHKPP
                     TLVQWRNATVVGALMLGGGMGGTAYAEQTIASGLVVAFIAITPALITLVSLPFGVRPS
                     RLEVVGIGVGMVGVFMLVRGDAFAASPAGLIAIVTGTLAWSLGSVLSQHVFPLAPSAT
                     GFSSEMLCGGAFLMVLSLVSGESFSWPPQTLATAAWLYLIVFGSLIAFCAYMVLLANA
                     SPALASSYSFVNPVIAMLLGVSLGGEIITTYEWLAAGGVALGVTVLVLARR"
     gene            complement(2858..4066)
                     /locus_tag="VFY27_05480"
     CDS             complement(2858..4066)
                     /locus_tag="VFY27_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5764998.1"
                     /translation="MERGIRLFRIAGVDIFLDWSLLIIFFLISSSLAMGLFPVWHPEW
                     SPALSWLTALAAATLFLLSVLVHEMAHALVGRANGMKISRITLFIFGGMAHLEDEPKH
                     WRVEFWMAIAGPLTSLALGFLFTWAGNLAAGGIDLTAGDPEAALAALGPAATLLLWLG
                     PVNIVLAVFNLVPGFPLDGGRVMRAVLWGITGNYQKATRWASTGGRIFGGLLIATGLA
                     MVLGIRVPFFGTGFTGGLWLALIGWFLHNAAAMSYTQLLMQRALSGVRVANIMQRNVE
                     TVSPDVALEEFVSDYVMGKDQRAWPVTEGDRLAGLVCLQDVRRRPREQWAATTVGEVM
                     TPADQLTTVLPDDDGWDAIRTINQKNVNQLPVMEGGRLRGLLRREDVVRWLSLHGDLP
                     AGRMSMNQPG"
     gene            complement(4162..4845)
                     /locus_tag="VFY27_05485"
     CDS             complement(4162..4845)
                     /locus_tag="VFY27_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5764999.1"
                     /translation="MNETTRSHNTVVITGANRGIGLAIARIYRERGDRVIALCRRASK
                     ELRDSGAEIVEDVDVTSPEALQQAAAAVGDVDVLVANAGVLSNESLSAVAGAVDRIER
                     QFRVNALGPLLTVHAFADKLASGARVALITSRMGSMADNTSGGSYGYRMSKAALNAAG
                     RSLANDLASRGIAVGLLHPGYVRTDMTGGRGDVEPAEAAARLVERIDDLSMANSGLFR
                     HANGEELPW"
     gene            4966..5427
                     /locus_tag="VFY27_05490"
     CDS             4966..5427
                     /locus_tag="VFY27_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5765000.1"
                     /translation="MTLVLTVNDTAHELDVDPQTPLLWVLRENLGLTGTKFGCGIAQC
                     GVCTVHMDGQPVRACITTAESAVGREITTIEGLSADASHPVQRAWIEHTVPQCGYCQS
                     GQIMAAAALLASTPDPSDADIDTAMAGHLCRCGTYPRIRTAIHHAAKLAKA"
     gene            5439..7625
                     /locus_tag="VFY27_05495"
     CDS             5439..7625
                     /locus_tag="VFY27_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5765001.1"
                     /translation="MTGSHTKVSRRTVIQAMAGVGGGMVLGFHIPNAVAAVVSPKPWT
                     TPANGVEINAWLAIDADGTVTIRVPHTEQGQGGMTSVAMMIAEELDVPWESVHAVFAD
                     MNRHVNDGGEYVVTSTHGSQLVRQQHPHIMQAGASARERLKEAAAKAWSVDRSDVTAR
                     QGILSSGSNRATYAKFATAAAGISLEREPAIKTPDRWWLLGKPTPRLDAAVKVDGSAR
                     YAIDARPEGMVYAAVKCCPVPWGRLVSYDFDSIRERRGVIAAVELESVPGRTDNSDMQ
                     NGVAVVADSWYRAKTALDALPIEWDFGPEGEVSSATQAAKAAELLDSTGSVSREDGND
                     TLGLIAASGNVISSEYHRPYETHARMEPINATVSVGSDRVDVWSPTQNQATALLLVAD
                     QLGRNTDDVHVHTAFIGGAFGGNGGGATAVTRQAAEIARQVGKPVKVIWSREEDMLQD
                     KQRAPVYARLSAVLGDDNLPEAFLSKSVIFARDAFGTSMADRGIASMPYRVPNRRHES
                     HGATAHIPTATHRAPGTNQNGFIIEQFADELAVAGNWDPLEWRLELTRGMEPWQRVLR
                     KLKEVAGFRTDLPRGQGMGIAVLEDHASYCAACATVSVSRGGELKVEKVVLVMNSGYL
                     INPRAAAEQMEGSVVWELSHALYGGLELERGQFVNTNFDSYKLMRMHQVPEIEVHFAM
                     SEDGWWGGIGEPAGPPTPPAVANAIWFATGKRVRSTPITRHDLSWS"
     gene            complement(7646..8347)
                     /locus_tag="VFY27_05500"
     CDS             complement(7646..8347)
                     /locus_tag="VFY27_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5765002.1"
                     /translation="MAKTTSRVLAEDIYSALKDDILNAVLRPGTMLDEMQLMQRFDVS
                     RTPIREVIRKLAADGLVGMEPHRSAYVNTFTVQDIADFFEAFRLTQRLVMVLSAARIN
                     RSQLDDILKLEQRLESACDTKKVRLARELNIQFHLNVAAGCSNKYLQESYGRLLEQST
                     RLSSLTFRLIVDRDWNLHSALILRNHNDIIDALVKRDCERIAALSDEHIAIFKKRVYE
                     ALDKDVPVAAVFSPV"
     gene            8694..11024
                     /locus_tag="VFY27_05505"
     CDS             8694..11024
                     /locus_tag="VFY27_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765003.1"
                     /translation="MSPLAAAIVAALHPVAFAVAQEDQRIEEIIVTSTKREINMQDLG
                     QSVTALTTADIQKQAIEGMEDYVRALPSVSLASEVPGRNTIVMRGMSAGTQEFYTDSQ
                     VAVYLDEQPITTISQELDVRMIDINRVEVLPGPQGTLFGSSSQSGTLRIITNQPDPTA
                     FSSEVDASVSTTKGGEASYDLSAWLNIPLIQDRFAIRAVAFTSHEGGYVDNVLGTTLE
                     GSETNADIAAEDQNSFDNVGGRIAARWNISDAWDLDLSYIAQQSALEGAWESDPALGD
                     YKITRFFDEYRDDDWYQVAGTIKGDLGFAELTATAAYFDRDIAYEWDNMVYEQWKTAF
                     WGVYYAVPLYDTDYTLSSIFNDQDIYRSSYEARLTSQGESRYQWMIGLFYEDLTNSWF
                     YGTRNPDYGGTTSWYAAQSYACYYANYGYDVQCPLPETDIGYSEDYYNSIKQTAVFGE
                     FSYDLTDRWTATLGARWFEFDRGTRYIYQFPQGLPPFGSFDTGGLVESSGVESDTVFK
                     LGTTFHLTDDVMLYALYSEGFRLGGNNSQRAANTGFVPLAYLPDKVANYEAGLKSRFA
                     DGRVLLNVIGFSVVWDQIQVNQGSVGGQWWLRGTINGGEAKSEGVELSAEWQATDNLH
                     LAASATFSDPRFTEDIALIGDVVPAGAPMVWSADRKYYLSLEYVVPDVLGGDLWFRYD
                     YSYEAEKWNNLSNIVENDRNGLVPSWELSNLHAGLTLQNGWEVQVDVRNAWDQLAYNS
                     LVNDSSGQFFGDPRFDNIRSYSRPRTIGLTVRKGFE"
     gene            11085..>12389
                     /locus_tag="VFY27_05510"
     CDS             11085..>12389
                     /locus_tag="VFY27_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5765004.1"
                     /translation="MTAVYYDAVIIGGGHNGLTCGAYLARAGRKVLVLERRPVIGGAA
                     VTEEFSPGFRASTFSYVMSLLHPKVIRDLGLCEPTLTVLPATDLFCPIGTDDYVIFSG
                     DVRRTAEQFARFSKHDGAIYAEFATWLNEAADVVRKLLLDTPVDPAKRDFRSLRDLLG
                     LVWKYRKIGNTFYRVVDLMTMSADDFLSRWFEHSVTRAVLGYYCSIGTFAGPKTPGSA
                     YVVLHHIMGEHEGAGGWGFIRGGMGAISEAIAESGRRHGMEIRTGAEVVRVEVAGGRV
                     SKVITGDGDEYRAHAVACNASCKVLFDRLIEPQHLPGEFLKHIRDYRTFSTAFKINVA
                     AEAPPRYRAFDRAKCDFEAPNYVHIGPTVEYLQAAYEDACHGRYSRRPFITAVVPTTV
                     DDTLAPAGKHVVNLFGGHAPYALNDATWDDEREGFVKTVFDTI"
BASE COUNT         2344 a         3884 c         3896 g         2265 t
ORIGIN      
        1 taggcgaccg ttcttgaagc ctcagtcatc caatcaataa ggcattgagt ctttggtgga
       61 ggtgtctgat gagagaactg gtgcttgtgt tggtggtcgc tgccggctgc acgacgatgc
      121 cgccgggaga gcgcgaagcg atggaataca aaaggaccga tgcgcgcctg aaggcaacgg
      181 aggagtacaa cgcgctccgg gcagggtgcc gtgcagcggg aggtgtcctc gtcgtggaag
      241 gtatggcgag ccgatggccg cgcacatcac tcgggatgaa gtccgcgcgc tgcatggaaa
      301 gtcggccccc cgtaattttc tgacggacgg caagtggcga ggcatcgctg gtgtccccga
      361 catgggtcgg tcgcgcgcgc gtccaggtcg tccgctcact ctgacgcgtc gccgtttatg
      421 aaaaccgcgg cagcacggtt tcagatgatg cagcagggaa gcacgaacgt cgacaggact
      481 gcacctcaac ccgctacaat cgccgcatgg gcaaatttat tgcggtactc cttctgctcg
      541 ccgcagcagc cggcgccgtt tggtatttcg ccggggaagc cgacccgccg gtcatcgaga
      601 ttgcacagcc ggcgcgcctg gtcggccaga ccggcgagct ggacgtcgct atcgcagcgc
      661 cgcgcggcga actcacgcgc ctccaggtca ctctccagca gggtacgacg cagctgccgg
      721 tgttcgctct tccgtcggcg ggatcggcgc agctcgcgcg ggaaagcgaa catcgcatgc
      781 gccttacggg gtcgatcggg aagcgcgaca ttccggacat cgtcgccggg ccgctgacaa
      841 tcgaggttac cgccgcgcgg ccggtgctgt tcggcctgcg agaagtagag tccactgcga
      901 cgcaggaggt ggaagttcgc ctcacgccgc cgcacgtgag cgtcgcctcg caatttcact
      961 acatcaatca cggcggctcc gagatggtcg tgtaccgcgt gcggccggag gacgtggaat
     1021 ccggcgtgca ggtcggcgag tacgagtatc ccggctaccc ggcgagcggc gccggcatag
     1081 caggcgcgga cccgggcctc cgggtggcgt tcttcgcgct gctgtgggac caggatgtcg
     1141 atacgccgat caatcttttc gcgcgcgacg aggtcggcaa cgaggcgcgg gcaaggttcg
     1201 actaccgcgt cttcccgaag aaatttcgct cgagccgcat caacgtgagc gacagtttcc
     1261 tgcaaaaggt tgtgccggag attctctaca acactccgga actcgaagtt cccgatccgg
     1321 acgaccttct tgcctcgtac ctgcagataa accgggagct gcgccggcaa aacaacgcgc
     1381 ggatcgcggc catggtcgcc gaaacgtctc ccgggattct ctggcgcggc gcgttcaagc
     1441 agctgacgaa cacggcagtc gaggcgggat tcgccgacca gcgcacctac gtgcacaacg
     1501 gcaacgacgt cgaccgccag gtgcacctcg gcttcgacct ggcttcgacc accaacgcgc
     1561 cggtgctggc ggcgaacagc ggtcgcgtcg tgcacgcggg ttggctcggc atctacggca
     1621 attgcgtgat cctcgatcac ggcatgggac tgcagtcgct ttatgcgcac ctttcgtcca
     1681 taagggtagg cgagggcgac gaggtcggta tgaacggcca gctgggcttg agcggcgaga
     1741 ccggcctcgc aggcggcgac catctgcact tcacgatgct gctggccggc cacgcggtga
     1801 cgccgatcga ctggtggagc cagcagtggg tcgatgaccg catcatgcga aaactacggc
     1861 aggcggcccc cggccaaccg gctcctgccg cgcgggcagg ccagtagagt cacgggtacg
     1921 cgcgttcccg ccgacaccaa agtcgctgct ggtgcaggcc ggctttcccc gctgattctc
     1981 gcctgccttg ccgctacctg gttcgtctgg ggatcaacct atttagcgat caagttcgcg
     2041 ctcgggagtt ttccaccgtt tttccagatg ggttcgcggt tcctggtcgc gggagcgctg
     2101 ctgatgctct gggtgaggtg gcgcgggcat aagcctccta cgctcgtgca atggcggaac
     2161 gcgaccgtgg tcggcgcgct catgctggga ggaggcatgg gcggaaccgc ttatgctgag
     2221 cagactattg cgtcgggcct cgtcgtcgct ttcatcgcca ttacgccggc gctgatcacg
     2281 ctggtcagcc tgccgttcgg cgtcaggcct tccaggctgg aagtcgtcgg catcggcgtc
     2341 ggcatggtcg gcgtgttcat gctggtccgc ggcgacgcat tcgcggcttc acctgccggt
     2401 ctgattgcga tcgtcacagg cacccttgcc tggtcgcttg gcagtgtctt gagccagcac
     2461 gtgtttcccc tggcgccctc ggccaccggc ttctccagcg agatgctctg cggcggagcc
     2521 tttctcatgg tgctgtcgct cgtgagcggg gaaagcttca gctggccgcc gcaaaccctg
     2581 gcgacagcgg cctggctgta cctgatcgtc ttcggttcgc tgatcgcctt ttgcgcttac
     2641 atggtgttgc tggcgaacgc gagtccggct cttgcctcca gctacagctt cgtcaatccc
     2701 gtcatcgcaa tgctgctcgg cgtttccctg ggcggcgaga taatcacgac ctacgaatgg
     2761 ctggccgcgg gcggagtcgc cctcggcgta acggtgctgg tgctggcgcg caggtaagag
     2821 cggccttctg cgtcagcaag aaccgaccgc gctttttcta tcccggctgg ttcatcgaca
     2881 tgcgcccggc cggcaggtcg ccgtgcagtg aaagccagcg cacgacatct tcccggcgaa
     2941 gcagtccgcg cagccttccg ccctccatca ccggcagctg gttaacgttc ttctggttga
     3001 tcgtgcggat tgcgtcccac ccgtcgtcat cgggcagaac ggtggtgagc tggtccgctg
     3061 gcgtcatcac ctcgccaacg gtggttgccg cccactgttc ccgcggccgc cgccggacat
     3121 cctgcaggca taccagcccc gcgaggcggt ccccctcggt caccggccac gcgcgctggt
     3181 ccttgcccat gacgtagtcg ctcacgaact cctcgagcgc cacatccgga cttaccgtct
     3241 cgacgttcct ttgcatgatg ttcgccacgc ggacgccgga cagcgcgcgc tgcatcagga
     3301 gctgcgtgta gctcatcgca gccgcgttgt gcaggaacca gccgatcagt gcgagccaga
     3361 gcccgccggt gaaacccgtg ccgaagaacg gcacgcggat accgagcacc atcgcgaggc
     3421 ccgtcgcgat cagcagtcct ccgaagatgc gcccgccggt ggatgcccag cgcgtcgcct
     3481 tctggtagtt gccggtgatg ccccacagca cggcgcgcat gacgcggccg ccgtcgagcg
     3541 ggaaacccgg taccaggttg aacactgcca gcacgatgtt gaccgggccg agccacagca
     3601 gcaaggtcgc cgcaggacca agcgccgcaa gtgcggcttc cggatccccg gcagtcaaat
     3661 cgatgccgcc ggcggcgagg ttgcctgccc aggtgaacaa gaagccgagg gcgaggctgg
     3721 tgagcggtcc ggctatcgcc atccagaact ccacccgcca atgtttcggc tcgtcctcga
     3781 ggtgtgccat tccgccgaag atgaaaaggg tgatgcgcga aatcttcatg ccgttggctc
     3841 gtccgacgag cgcatgcgcc atctcgtgca cgagcacgga cagcaggaac agcgtcgccg
     3901 ccgcgagagc ggtgagccag ctcaaggccg ggctccactc cgggtgccag acaggaaaca
     3961 ggcccatcgc aaggctcgag ctgatcagga agaagatgat cagcaggctc cagtcgagga
     4021 agatgtcgac gcccgcgatt cggaaaagcc tgatgccacg ttccatagcc gctctcactt
     4081 gatgggctca acgaattgca tttggtattg cataattcat accgtgcggg ggaggctgct
     4141 ctgcaaatcc ggcgatagcc gctaccaggg tagctcttcg ccgttcgcgt gcctgaacaa
     4201 accgctgttg gccatgctca ggtcgtcgat cctttccacg agccgtgccg cggcctcggc
     4261 aggctcgacg tccccgcgac cgccggtcat gtcggtacgg acatagccgg gatgcagcag
     4321 gccgaccgca atcccgcgcg atgccaggtc gttcgccagg gaacggccgg cggcgttcag
     4381 tgccgccttc gacatcctgt aaccgtaact gccgccggaa gtattgtccg ccatcgatcc
     4441 catccggctc gtgatcagcg cgaccctcgc gccgctggcc agcttgtcgg cgaaggcatg
     4501 gacggtcagc agcggcccga gcgcgttgac ccgaaactgc cgctcgatcc ggtccactgc
     4561 cccggcgacg gcggacaagg attcgttact cagcacgcct gcgttcgcaa ccagcacgtc
     4621 tacgtccccg acggccgctg cagcctgctg taacgcttcc ggagacgtga cgtctacgtc
     4681 ttcaacgatc tcggcgccgg aatcgcgtag ctctttgctg gcgcgccggc acagcgcgat
     4741 gacgcggtcg ccgcgctccc ggtaaatccg cgcgatcgca aggcctatgc cccggttcgc
     4801 gccggtgatg acgacggtgt tgtggctgcg ggtcgtttcg ttcatgggat ctcctcgggc
     4861 taaagggtat tggcccgccg tttgcactgg cgggcgcgtc ggcgcatagt tttaaaggac
     4921 ggcacgaacg atgcaaagtg tgttccaacc tgcggaagcc cgcccatgac cctcgtactg
     4981 acagtcaacg atacagctca cgagctcgac gtcgatccgc agacgccgct gctctgggtt
     5041 cttcgcgaaa acttagggct caccggcacg aagttcggct gcggcatcgc ccagtgcggc
     5101 gtctgcaccg tgcacatgga cgggcagccg gtgcgcgcct gcatcacgac cgccgaaagc
     5161 gccgtcggcc gggagatcac gacaatcgaa ggcctgtcag ccgatgcaag tcatcccgtg
     5221 cagcgggcat ggatcgaaca taccgtcccg cagtgcggct attgccagtc cgggcagatc
     5281 atggcggcag ccgcgctgct cgcaagcacg cccgatccga gcgatgcgga catcgacact
     5341 gcaatggcag gccacctttg ccgctgcggc acgtatccgc ggatacgcac ggccattcat
     5401 cacgccgcca aactggcgaa agcgtgaagg aggccgcgat gacaggaagc cacacgaaag
     5461 tctcgcggcg caccgtcatc caggcgatgg ccggggtcgg cggcggcatg gttctcggtt
     5521 tccacatccc caatgctgtt gccgcagtgg tctcgcccaa accatggacc accccggcga
     5581 acggcgtcga gatcaacgcg tggctggcga tcgacgccga cggcaccgta acgatccggg
     5641 tgccgcatac cgagcagggg cagggcggca tgacctccgt tgcgatgatg atcgccgagg
     5701 aactggacgt gccgtgggag agcgtccacg ccgtcttcgc ggacatgaat cgtcacgtca
     5761 acgacggggg cgaatacgtc gtcacctcga ctcacggctc gcaactcgtg cgccagcagc
     5821 acccgcacat catgcaggca ggcgcttccg cccgcgaacg tttgaaggag gcggcggcca
     5881 aagcgtggag cgtcgaccgc agcgacgtca cggcaaggca gggaatcctg tcgtcaggca
     5941 gcaaccgcgc gacgtacgca aaattcgcaa cggcggcagc cgggatcagc ctcgaacgtg
     6001 agcccgcgat caagacgccg gaccggtggt ggttgctcgg caaacccact ccgcgcctgg
     6061 acgcagcggt gaaggtcgac ggcagcgccc gatacgcgat cgacgcgcga ccggaaggta
     6121 tggtatacgc cgccgtgaaa tgctgccccg tcccctgggg acggctcgtg agttacgact
     6181 tcgacagcat ccgcgagcga cgcggcgtga ttgccgccgt cgagctcgag tccgtgccgg
     6241 ggcgcacgga caactctgac atgcagaacg gcgtcgccgt tgtcgcagat tcctggtacc
     6301 gcgcgaagac cgcgctggat gcgctgccga ttgaatggga tttcggtccg gaaggcgagg
     6361 tcagcagcgc gacgcaggcc gccaaagcgg ccgagcttct ggactcgacc ggcagcgtga
     6421 gccgcgagga cgggaatgac acgctcggcc tcatcgccgc gtccggcaac gtcatctcga
     6481 gcgaatacca ccgtccctac gagacgcacg ctcgcatgga accgatcaac gccaccgtga
     6541 gcgttgggtc cgacagggtc gacgtgtggt cgccgacgca gaaccaggcc acggcgcttc
     6601 ttctcgtcgc cgaccagctc ggcaggaata cagatgacgt gcacgtgcac acggctttca
     6661 tcggcggtgc gttcggcggc aacggcggcg gcgcgacggc cgtgacgcgg caggcagccg
     6721 agatagcgcg ccaggtcggc aagccggtga aggtcatctg gtcgcgcgaa gaagacatgc
     6781 tgcaggacaa gcagcgcgcg ccggtgtacg cgcggctatc cgccgtgctc ggcgacgaca
     6841 atttgccaga agccttcctc tcgaagtccg tgatctttgc gcgcgacgca ttcggcacca
     6901 gcatggcgga tcgcggtatc gcctcgatgc cctaccgtgt gccgaaccgc cgccacgaaa
     6961 gccacggcgc caccgcacat atcccgacgg cgacgcatcg cgcgccggga accaaccaga
     7021 acggcttcat aatcgagcag ttcgccgacg agctggcagt ggccggcaac tgggatccgc
     7081 tcgagtggcg cctcgagttg accaggggca tggagccctg gcaacgcgtg ctcagaaagc
     7141 tgaaggaagt tgccggcttt cgcaccgacc tgcctcgcgg ccagggcatg ggaattgcgg
     7201 tactggaaga ccacgcttct tactgcgccg cctgcgcaac cgtctccgtc agtcgcggcg
     7261 gcgagctgaa ggttgagaaa gtcgtactag taatgaacag cggctacctg ataaatcccc
     7321 gtgccgcggc ggagcagatg gaaggctcgg tcgtctggga gctctcgcac gccctgtacg
     7381 gcggactgga actcgagcgc ggccagttcg tgaacacgaa cttcgacagc tacaagctca
     7441 tgcgcatgca ccaggtgccc gagatagaag tccatttcgc gatgtcagag gatggctggt
     7501 ggggcggcat cggcgaaccc gcagggccgc caactccgcc agcggtcgcc aacgcgatct
     7561 ggttcgccac gggcaagcgc gtgcgctcga cgccgattac gaggcatgac ctgagctggt
     7621 cgtagcgaag ggttcgatgc gctactcaga cgggactgaa gacggcagcg accggaacgt
     7681 ccttgtcgag cgcctcatac acccgcttct tgaatatcgc tatgtgttcg tcggaaagcg
     7741 cggcaattct ttcgcagtcg cgcttcacca gcgcatcaat gatgtcgttg tgattccgca
     7801 gtatcagagc cgagtgcagg ttccagtccc tgtcgacgat caggcggaag gtcagtgacg
     7861 acagtcgcgt gctttgctca agtagtcggc cgtaggattc ctggaggtat ttattcgaac
     7921 agccggcggc aacgttcaga tggaactgga tgttcagttc ccgtgccagc ctgacctttt
     7981 ttgtatcgca agccgactcg agccgctgct ccagtttcag aatgtcgtcg agctgactgc
     8041 ggttgatcct tgccgcgctc agcaccatca ccaggcgttg agtcaaacgg aaggcttcga
     8101 agaagtcggc gatgtcctgc accgtgaacg tgttgacgta ggcactacgg tgcggctcca
     8161 ttccgaccag gccgtcggcg gccagtttgc gaatcacttc ccggattggc gtgcggctga
     8221 catcgaagcg ctgcataagc tgcatctcgt ccagcatcgt gccgggcctg agtaccgcat
     8281 tcaggatgtc gtctttcagc gcgctgtaga tgtcttcggc aagcacccgg gacgtcgttt
     8341 tagccattct tcgctctcct caggttgcaa gatagcctcc ggtttcagat gaatccagat
     8401 gcgattgcct tggtctgcta ccaatattgt attcttattg tatatcgact acattgtcga
     8461 tcagtcccgg aaaagatcgc cagcttagcg gagtaccttc caagaaaacc tacacagtta
     8521 gatttcgcag gcgccttgtg tacaatttgt acatacaccc gcagcgcatt gcagggcgcc
     8581 atcggagaga tccagtgaac agaacgcctt cccctggctg cacgtcggac agcgcgccga
     8641 ttggtcccgc acgtccccca catcccgcca cttccgcata ttgcgcgcgc ggagtgagcc
     8701 ccctggcggc cgcgatcgta gcagccctgc atccagtagc gttcgcggtg gcgcaggagg
     8761 accagcggat cgaggaaatc atcgtcacgt cgaccaagcg tgagatcaac atgcaggatc
     8821 tcgggcaaag cgtcaccgcg ttgacgacgg ctgacatcca gaagcaggcg atcgagggga
     8881 tggaagacta tgtccgggcg ctaccaagcg tatcgctcgc gagcgaggta ccaggccgca
     8941 acaccatcgt gatgcgcggc atgtcggcgg gaacgcagga gttctatacc gacagccagg
     9001 tggccgttta cctcgacgag cagcccatta cgaccatttc ccaggaactc gacgtaagga
     9061 tgatcgatat caaccgcgtg gaggttttgc cgggaccgca gggtacgctg ttcggctcga
     9121 gttcccagtc cggcacgctg cgcatcatta cgaaccagcc ggatcccacg gcgttctcga
     9181 gtgaggtcga tgcctctgtc tcgactacca agggcggtga ggcaagttat gacctgagcg
     9241 cctggttgaa cattcccctc atccaggaca ggtttgcgat ccgagcggtc gccttcactt
     9301 cccacgaagg gggctatgtc gataacgttc tcggaacgac cctcgaaggc tcggagacca
     9361 atgcggacat cgccgcggag gatcagaact cgtttgacaa tgtcggtggc cggattgccg
     9421 cgcgctggaa catcagcgat gcatgggacc tcgacctgtc gtatatcgcg cagcaaagcg
     9481 cgctggaagg tgcctgggag tccgacccgg cccttggcga ttacaagatc acccgttttt
     9541 ttgatgagta cagggacgac gactggtatc aggtagccgg cacgatcaag ggggatctgg
     9601 gcttcgccga gctcacagcc acggccgcct atttcgatcg cgacatcgct tacgaatggg
     9661 acaacatggt ctatgagcag tggaagaccg cgttctgggg ggtttactac gccgtcccgc
     9721 tttacgacac ggactatacg ctcagctcga tctttaacga tcaggatatc tatcgatcgt
     9781 cctatgaggc gcggctgacg tcgcaaggag aaagccgata tcagtggatg atcggcctgt
     9841 tttacgaaga cctgacgaac agctggttct acggcacgag gaatcccgat tatggcggca
     9901 caacctcttg gtatgcggct caaagctacg cctgctacta cgcgaactac gggtacgacg
     9961 tgcaatgccc gctgccggag acagacatcg gctactcgga ggactactac aactccatca
    10021 agcaaacggc ggtattcggc gagttcagtt atgacctgac cgaccgatgg acagcgaccc
    10081 tcggcgctcg ctggttcgag ttcgatcgtg gcactcgtta catctaccag tttccgcagg
    10141 ggctgccacc cttcggttcc ttcgacaccg gcgggcttgt cgagtcctct ggcgtggaaa
    10201 gcgacaccgt attcaagctg ggaacgactt tccatctgac cgatgatgtg atgctttatg
    10261 ccctgtacag cgaaggcttc cgcttggggg gcaacaacag ccaacgagcc gcgaacacgg
    10321 gcttcgtgcc gctcgcgtat ttgcccgaca aggtcgcgaa ttatgaagcc ggcttgaaaa
    10381 gtcggttcgc tgacggccgt gttctactga acgtcatcgg cttcagcgtc gtctgggacc
    10441 agatccaggt caaccagggc agcgtcggag gccaatggtg gctgcgtggc acgatcaacg
    10501 ggggcgaggc gaaaagcgag ggcgtggaac tcagcgccga atggcaggcc acggataact
    10561 tgcacctcgc cgccagcgcc accttcagcg accccagatt cacggaagac atagctctta
    10621 tcggcgatgt cgttcctgcg ggagcaccga tggtgtggtc ggcagaccgt aagtactacc
    10681 tcagcctgga atacgttgtg cccgacgtcc ttggcggcga tctctggttc cgctacgact
    10741 acagttacga agccgagaaa tggaacaatc tctccaacat cgtggaaaat gatcgcaacg
    10801 gactggtgcc ctcctgggaa ctgagtaatc tccatgccgg gctgaccctg cagaacggct
    10861 gggaggtgca agttgacgtc agaaacgcct gggaccagct tgcgtacaac tcgctggtta
    10921 acgatagcag tggtcagttt ttcggtgacc cacgcttcga caacattcgc agctactcca
    10981 ggccgcgcac catcggcctg accgtgcgca agggattcga gtgaaacgcg tcgcggaccc
    11041 tgtcttgaag ttccgtgcat gagggcagcg gcgctgtcgg gtcgatgacc gctgtttact
    11101 acgatgccgt catcatcggc ggcggccaca acggcctcac ctgcggcgcc tatcttgccc
    11161 gggccggcag gaaagtcctg gtgctcgaac gccgcccggt gatcggtggc gcggctgtga
    11221 ccgaggaatt ttcacctgga ttccgggcgt cgaccttctc ttacgtcatg agcctgttgc
    11281 atcccaaggt gattcgtgac ctcggactgt gcgaaccgac gttgacggtg ctaccggcaa
    11341 ccgacctgtt ctgcccgata ggcaccgatg actacgtcat cttcagcggt gatgtgcgca
    11401 ggacagcaga gcagttcgcc cgcttttcga agcatgacgg cgcaatctac gccgagttcg
    11461 ccacctggct caacgaggcg gcggacgtcg tgcgcaaact gctgctcgac acgccggtcg
    11521 atcccgcgaa acgcgatttt cgaagcctgc gcgatcttct cggactggta tggaaatacc
    11581 gcaagatcgg caacaccttc taccgggtgg tcgacctgat gaccatgagc gctgacgact
    11641 tcctgtcccg ctggtttgag cacagcgtca cccgcgccgt gctgggctat tactgctcca
    11701 tcggcacgtt cgccggaccg aaaacgccgg ggtcagccta cgtcgtcctg catcacatca
    11761 tgggcgaaca cgaaggcgcc ggcggctggg gcttcatacg cggtggtatg ggcgccatca
    11821 gtgaggctat cgcggaaagc ggccgacgcc atggcatgga aatccggacc ggcgccgagg
    11881 tcgtccgggt ggaggtcgca ggtggacgtg tttccaaggt catcacgggg gacggcgatg
    11941 aataccgcgc gcacgccgtt gcgtgcaacg caagctgcaa ggtcttgttc gacaggctca
    12001 tcgagccgca gcatctgccc ggagagttcc tgaaacacat tcgggattac cgaacttttt
    12061 ccacggcttt caagatcaac gtggccgccg aggcgccgcc acgctatcgg gcattcgacc
    12121 gcgcgaagtg cgatttcgaa gcgccgaatt acgtgcacat cggccccacc gtcgaatacc
    12181 tgcaggccgc ctatgaggac gcctgtcacg ggcgctactc gcggcgcccg ttcatcacgg
    12241 cagtcgtgcc gactactgta gacgataccc tcgctccagc cggcaagcat gtcgtcaacc
    12301 tcttcggcgg ccatgcccct tacgcgctga atgacgcaac ctgggatgac gaacgggaag
    12361 ggttcgtgaa gacggtgttc gacacgatc
//
LOCUS       DASUOM010000264         5953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1454518,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000264 DASUOM010000000
VERSION     DASUOM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454518"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VFY27_05515"
     CDS             complement(<1..1157)
                     /locus_tag="VFY27_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HEX5765005.1"
                     /translation="MQELLDFLNGIIWSPVLVYLCLGAGLFFSLLTRFVQVRLFGEMV
                     RLLFTGRATATGISSFQALAVSLSGRVGTGNIAGVAAAIGFGGPGAVFWMWVVAFLGA
                     STAYVESALGQIYKEEDEGQFRGGPAYYIEKAMGQKWYAWIFAITTIFATGMLLPGVQ
                     AYSISSSAEVAIGTGQLIDTDFGVISVTKIVTGTAVVTLLGFIIFGGVRRIAAFAQFA
                     VPFMAVGYIIAACVIVILNITMLPEVVALILKDAFTPMAGAGAAIGWGVKRGVYSNEA
                     GQGTGPHAAAAAEVQHPAQQGLVQAFSVYIDTLFVCSATAFMILITGAYNVYGGDGAL
                     IVQNLPAEIEANSPAFTQFAVDSMMPGVGKPFVAVALFFFAFTTLLAYYYIA"
     gene            complement(1318..2214)
                     /locus_tag="VFY27_05520"
     CDS             complement(1318..2214)
                     /locus_tag="VFY27_05520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5765006.1"
                     /translation="MILRRLNEFFAGQSAYMIGGVSFALILLLAGIDHMTGYELSVSV
                     FYLIPVGVATWYGNRQLGYIVSGLSAGTWLVVEQATAQPYSHDWILFWNSAVRLLAFA
                     VVAFLLAELRTLLQRQQELARTDSLTGLLNRTGFLERASIATTSAARYGYSVAVAYID
                     LDDFKNINDSLGHEQGDAVLTAVGKLLAKSSRDSDVVARIGGDEFVVMLPNTSLAGAR
                     AYFEKMRFELQMKIRQQGWKTLGLSVGAVVFDKAPEDIFEALRLADKLMYRAKRASNA
                     GVIVERAARMPAAVLRRAAIDA"
     gene            complement(2405..2890)
                     /locus_tag="VFY27_05525"
     CDS             complement(2405..2890)
                     /locus_tag="VFY27_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5765007.1"
                     /translation="MITIETSKGDIVIELFEEEAPLSSANFAQYAEDGFFDDTVFHRV
                     IPNFMIQGGGMTEDLSSKKTRAPIRNEAANGLKNLRGTLAMARTGQVDSATSQFFINL
                     RDNSFLDHGGRDYGYAVFGRVSDGMDVVDAIGKVETGRQKGHEDVPLEPVVIRKVSIS
                     D"
     gene            complement(2984..3814)
                     /gene="prmC"
                     /locus_tag="VFY27_05530"
     CDS             complement(2984..3814)
                     /gene="prmC"
                     /locus_tag="VFY27_05530"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5765008.1"
                     /translation="MKIDAAIENAREKLETVSDSPRLDAEVLLARALDLPRSYLFAHP
                     DEVLDDASMERFLHAVEQREQGMPLSYITGEKEFWSLSLEVSQAVLVPRPETELLVEL
                     ALGEIPRNDGYFVLDLGTGSGAIAIALASERPACRFVASDLSPAALAVARHNAMKHGL
                     ANIHFVEGDWTAPVADQRFNLIVSNPPYVGEGDPALSRLRFEPKEALVAGNDGLDAIR
                     TLARNCRRLLERGGLLLLEHGAKQQAEVAGILADEGWTGVQNFKDLSGLPRATRAVRG
                     "
     gene            3934..5295
                     /locus_tag="VFY27_05535"
     CDS             3934..5295
                     /locus_tag="VFY27_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5765009.1"
                     /translation="MTIFSNVLEMVGQTPMLELTRFDTGSCRLFLKLELLNPGGSIKD
                     RIGISMIEEAERRGDIRPGDTIVEATAGNTGLGLALVAAQKGYRLVLVLPDKMSQEKI
                     FNLRAMGAEVVLTRSDVGKGHPEYYQDLGKAIAEEQGAYFINQFGNPDNPLAHERTTA
                     PEIFEQMGGQVDAIVLGVGSSGTVSGISRYLEQHAADAELVLADPVGSVLADYINKGE
                     LGEKSGWLVEGIGEDFIPSIADFSRVKKAYSIGDAESFSTARELLRREGILAGSSTGT
                     LLAAALKYCREQEAPKRVVTFAADTGNKYLSKLFNDFWMQDQGFIERESYGDLRDLIG
                     RPHGERATITVGPTDVLTTAHNRLRNAGFSQLPVMHEGKLVGVLTEDAIIRFVFGHPD
                     LLHRPVKDAMQTAFIQLDKDTSINNLVAMLQVQPYAAVLDKDQFLGLITRSDVLNYLR
                     KQS"
     gene            5314..>5953
                     /locus_tag="VFY27_05540"
     CDS             5314..>5953
                     /locus_tag="VFY27_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5765010.1"
                     /translation="MKDKANNSRHGFGTRAIHAGQKPDPSTGAIMQPIYATSTYVQKS
                     PGVHQGYEYSRTQNPTRQALESCTADLESGRFGFAFSSGMAATATILELIDSGSHVIA
                     MDDIYGGTTRLFERVRRRSAGLDFTFVDLTDVSALQKAVKSNTRMIWIESPSNPLLKV
                     VDFRLVTEFARARNVLVVADNTFATPCLQRPLEFGCDIVMHSVTKFLNGHSDM"
BASE COUNT         1227 a         1842 c         1803 g         1081 t
ORIGIN      
        1 gcgatgtagt agtaggcaag aagcgtcgtg aacgcgaaga aaaacaatgc taccgcgaca
       61 aagggcttac cgactcccgg catcatgctg tcgaccgcga attgggtgaa cgccgggctg
      121 ttcgcctcga tctcggcggg caggttttgc acgatcagcg cgccatcccc gccgtagacg
      181 ttgtaggctc cggtaatgag aatcatgaag gcggtcgcag agcatacgaa cagcgtgtcg
      241 atgtagacag aaaatgcctg gacaaggcct tgctgcgcgg gatgctgcac ctccgcggcg
      301 gctgctgcat gcggtcccgt accctgacca gcctcgttgg aatagacgcc gcgcttgaca
      361 ccccagccga tggctgcccc ggcacccgcc attggcgtaa acgcatcctt gagtatcagc
      421 gcgaccacct caggtagcat tgttatattc aggatcacga tcacgcatgc cgcgatgatg
      481 tatccaaccg ccatgaaggg aaccgcgaat tgcgcgaagg ccgcgatacg cctgacgccg
      541 ccgaaaatga tgaaaccgag caaggtaacc accgccgtac cggtcactat tttagtgacg
      601 ctgatgacgc cgaagtccgt gtcgatgagc tggccggtgc ctatggcgac ttccgcggac
      661 gaactgatgc tgtaggcctg cacgccgggt aacagcatgc cggtggcgaa gatagtcgtt
      721 atggcgaaga tccacgcgta ccacttctgc cccatcgctt tttcgatgta ataggccgga
      781 ccaccgcgga actgtccttc gtcctcctcc ttgtatatct ggcctagagc agattcgacg
      841 taggcggtag acgcaccgag gaacgccact acccacatcc agaacacggc ccccggtccg
      901 ccgaaaccga tggccgcggc aacacccgcg atgttgccgg taccaacccg ccctgagagg
      961 gacactgcga gggcctggaa ggaagagatg ccggtggccg tggcgcgtcc ggtgaacagc
     1021 aaacgtacca tctcaccgaa aaggcgcacc tggacaaagc gcgtcagcag agagaagaac
     1081 agcccggccc cgaggcacag gtagacgagc accgggctcc agataatgcc gttcaaaaaa
     1141 tccagtagtt cctgcatgtc ccccgcctgt gccagtgccc ggtgagatta ttttgcggca
     1201 tggtacctga acaggcgtcc agcaccacag ggcgtcacca gggttgatcc gcaagaagag
     1261 caaggatcgg gcgctgaggg agcccgtcct ggaggcgaag gagcaccagc cgcaagatca
     1321 ggcgtcgatt gcagcgcgac gcaatactgc tgccggcatg cgagctgcac gctctacgat
     1381 gaccccggcg tttgacgcgc gcttggcgcg gtacatcagc ttgtcggcca gtctcaatgc
     1441 ttcgaagata tcttcaggcg ctttgtcgaa tacgacagca cccacgctca agccgagggt
     1501 tttccacccc tgttgccgaa ttttcatctg cagctcgaac cgcatctttt caaagtacgc
     1561 acgggcgcca gccaggctgg tattcggcag catcacgacg aactcatcgc ctccgatacg
     1621 cgcaacaacg tcactgtcgc ggctcgattt tgcaagaagc ttgccgacgg ccgtcaggac
     1681 ggcgtcgccc tgctcgtggc ccagcgaatc gttgatgttc ttgaaatcat ccaggtcgat
     1741 gtacgcgact gcaaccgagt agccgtaccg tgccgcactg gtggttgcaa tactcgcgcg
     1801 ctccaaaaag ccggtgcggt tgagcaggcc ggtgagacta tcggttcgtg caagttcttg
     1861 ctggcgctgc agaagcgttc tcaactctgc aaggaggaag gcgacgactg cgaatgccaa
     1921 cagccgcacg gcgctgttcc agaaaagtat ccagtcgtgg gaatacggtt gcgcagtcgc
     1981 ctgttcgacg accagccagg tccccgcgga caggccgctg acgatatagc cgagctggcg
     2041 attgccgtac caggtcgcca cgccgaccgg aatcaggtag aagaccgata cggagagttc
     2101 gtagccggtc atatggtcga ttcccgcgag caacaggatc aacgcaaacg acactccgcc
     2161 aatcatatat gcagactggc cggcgaagaa ttcgttgagc ctccggagaa tcacatccgc
     2221 tcccccaaag cagaaagtgt accccgtcgg cgtgcttgtc tctaccgaaa tccggacgtt
     2281 acgtcatatc gctcacacag gttcgacgcg cgatgcgagc cggcgacgga aaggagcgct
     2341 ttgcgcacga caggtagttg caatcgaaag tgatcaggcg agcgctcccg ccgccgcagc
     2401 accgtcagtc gctgatgctc accttccgga tcacgacggg ctccagcggc acgtcctcgt
     2461 gccctttctg gcggccggtt tcgactttcc cgatcgcatc gacgacgtcc atgccgtcgc
     2521 taaccctgcc gaacacggcg taaccgtagt cccggccgcc atggtcgagg aagctgttgt
     2581 cccggaggtt gatgaagaac tgcgaggtgg cgctgtcgac ctggccggtc ctcgccatcg
     2641 caagcgtgcc cctcaggttt ttcagcccgt tggccgcctc gttcctgatt ggcgcgcggg
     2701 tttttttcga cgagaggtcc tcggtcatgc cgccaccctg gatcatgaaa ttcgggatga
     2761 cccggtggaa gacggtgtcg tcgaagaaac cgtcttcggc gtactgcgcg aagttcgcgc
     2821 tgctgagcgg cgcctcttct tcaaaaagct cgatgacgat atcgcccttg ctggtctcga
     2881 tggtgatcac tgcgggttcc cattggctaa aaagagcgtg acagtataga agctcgatca
     2941 aaatttgcac gtgccgaccg gcacggcatc agccgttgtc ctgctagccg cgaacggccc
     3001 gcgtcgcccg tggcaggccg gaaaggtcct tgaaattctg gacaccggtc cacccttcat
     3061 cggcaagaat gccggcgacc tctgcctgct gcttcgcacc atgctcgagc aggagcagcc
     3121 cgccgcgctc gaggaggcgc cggcagttgc gcgcgagcgt gcggatggcg tcgaggccgt
     3181 cattgccggc aacgagagct tccttaggtt cgaagcgcaa gcgggaaagg gcaggatcgc
     3241 cctcaccgac gtatggcggg ttcgagacga tcaggttgaa acgctgatcc gcaacggggg
     3301 cagtccagtc gccctccacg aaatgaatat tggcaagccc gtgtttcatt gcgttgtgcc
     3361 gggcaacggc gagggcggca gggcttaagt cgctcgcaac aaaacggcat gcgggccgct
     3421 cgctcgcgag tgcgatcgcg atagcgccgc ttccggtgcc gagatccagg acaaaatacc
     3481 cgtcgttccg cgggatctcg cccagcgcca gctcgaccag gagttccgtc tccgggcgcg
     3541 gcaccagcac cgcttgcgat acctcgagcg acagcgacca gaattccttt tcgccggtaa
     3601 tgtaggacaa gggcattccc tgttcccgct gttccactgc gtggaggaag cgctccatcg
     3661 aagcatcgtc caggacctcg tccggatggg cgaaaaggta gctgcgcggt agatccagcg
     3721 cgcgagcaag cagtacttcc gcatccagtc gcgggctgtc gctcaccgtc tcgagctttt
     3781 cccgggcgtt ttcgatggcg gcatcgattt tcaactcaag cctcctgcag ctcgaggtag
     3841 ctctggccgc cgcaggctag agctaccacg gggcttggcg tagtatggcc cgcactttcg
     3901 attgcaactt ccacgggcat cccgccattc cctatgacga ttttttccaa cgtgctggag
     3961 atggtcgggc agacgccgat gctcgagctg acccggttcg ataccggttc ctgccgcctg
     4021 ttcctcaagc tcgaactgct gaacccgggc ggctccatca aggatcgcat cggcatctcg
     4081 atgatcgagg aagccgaacg ccgcggcgac atccgccccg gggacacgat cgtcgaagcg
     4141 acggccggta acaccggtct cggcctcgcg ctggtcgcag cacagaaggg gtaccgcctg
     4201 gttctcgtgc tgcccgacaa aatgagccag gaaaaaatct tcaacctgcg cgcgatgggc
     4261 gcggaggtcg tactgacgcg ttccgacgtg ggcaaagggc accccgagta ctaccaggat
     4321 ctcggcaagg ctatcgcgga ggagcagggc gcgtacttca tcaaccagtt cggcaatccc
     4381 gacaacccgc ttgcgcacga gcgtaccacg gctcccgaga tcttcgagca gatgggcggg
     4441 caggtggacg cgatcgtgct cggcgtcggc tcgagcggca ccgtcagcgg catcagccgc
     4501 tacctcgagc agcacgcggc ggatgccgag ctcgtgctcg cggacccggt cgggtcggtg
     4561 ctggcggact acatcaacaa gggcgagctc ggcgagaaga gcggctggct cgtcgaggga
     4621 atcggcgagg atttcatccc atccatcgcg gatttcagcc gggtgaaaaa ggcctacagc
     4681 atcggcgatg ccgagtcgtt cagcacggct cgcgaattgc tgaggcgtga gggcatcctt
     4741 gccggttcgt cgacaggcac gctactggcg gcggcgctca agtactgccg cgagcaggaa
     4801 gcaccgaaac gcgtggtcac ttttgcggcg gataccggta acaagtacct gtcgaagctg
     4861 ttcaacgact tctggatgca ggaccagggc ttcatcgaac gagagtccta tggggacctc
     4921 cgcgacctga tcgggcgacc gcatggcgag cgcgcgacga tcacggtagg tcccacggac
     4981 gttctcacca ctgcgcataa ccggctgcgc aatgccggct tctcgcaact gccggtcatg
     5041 cacgagggca aactggtggg cgtgctgacg gaggacgcca tcatccgctt cgtgtttggt
     5101 catccagatc ttctgcaccg tcccgtgaag gacgcaatgc agaccgcatt catacagctc
     5161 gacaaggaca ccagcatcaa caacctggtg gcgatgctgc aagtgcagcc gtacgctgca
     5221 gtgctggaca aggaccagtt tctcgggctc attacgcggt ccgacgtctt gaactatctg
     5281 cgcaagcaga gctgaaggac ctacgaagga cctatgaagg acaaagcgaa caacagcagg
     5341 cacggcttcg gcacccgcgc catacacgca gggcagaagc cagacccctc caccggcgcc
     5401 atcatgcagc cgatctacgc gacatcgacc tacgtacaaa agagtcctgg cgtgcaccag
     5461 ggttatgaat actcgcggac gcagaatcca acgcgccagg cgctggaaag ttgtactgcg
     5521 gacctggagt ccggccgctt cggtttcgcg ttcagttccg gcatggccgc gacggcgacg
     5581 atcctcgagt tgatcgactc cggcagccat gtcattgcga tggacgacat ctatggtggc
     5641 accacgcgtc tgttcgaacg cgtgcgcagg cgcagtgcag gccttgattt caccttcgtc
     5701 gacttgaccg acgtgtccgc cttgcagaag gcggtgaaga gcaacacgcg catgatctgg
     5761 atcgagagtc ccagcaatcc cctgctgaag gtcgtcgact tcaggctggt gacggagttc
     5821 gccagggcgc gcaacgttct cgtcgtcgcc gacaatacct tcgccacgcc ctgcctgcag
     5881 cgccccctgg agttcggctg cgacatcgtg atgcactcgg tgacgaagtt cctgaatggc
     5941 cactcggaca tgg
//
LOCUS       DASUOM010000265         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1625684,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000265 DASUOM010000000
VERSION     DASUOM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625684"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1376)
                     /gene="gabT"
                     /locus_tag="VFY27_05545"
     CDS             complement(78..1376)
                     /gene="gabT"
                     /locus_tag="VFY27_05545"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX5765011.1"
                     /translation="MTTNQQLSARRDSAIPRGVSNLHRVFAARSKNAELWDVEGRRYI
                     DLAAGIAVLNVGHNHPRVLAAVHAQLEQFTHTCFQVTPYEPYVKLAERLNALVPGKTP
                     KKTLFVTTGAEAIENAVKIAKYHTRRSGVVSFSGAFHGRTMMGMALTGKVAPYKAGFG
                     PFPGDVYHIPYPVAYHGTTPAQSLRALEDLFRSDIEPSRIAALIIEPVQGEGGFYAAP
                     AEFLRSLRTICDEHGIMLVADEVQTGFGRTGKIFAIEHAGIEADLVTVAKSLGGGFPI
                     AGVIGKAEVMDSVPPGGLGATYGGPPVGCAAGLAVLDIIAEEGLCERADAIGRRIVYW
                     GTALQGKNSSIGDVRTVGAMSAIELVRDGDASRPDPELTKAVVAEAMKQGVILLSCGA
                     RGNVIRFLPPLTISDRLLDDALGIVGNVILGLALEVRKAG"
     gene            1624..>2190
                     /locus_tag="VFY27_05550"
     CDS             1624..>2190
                     /locus_tag="VFY27_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5765012.1"
                     /translation="MRDMLQFYIDGRWVDPVTPKTVNVINPATEEVVGRISMGSAEDV
                     ERAVAAAKAAFPAYSTTTRDERTALLEKILEVFAKRHDEVATAIMEEMGAPWKLAKHA
                     QAASGPQHIQAAIEVLRTFDFEETLGTTRVLKEAIGPCALITPWNWPMNQVAVKVAPA
                     LAAGCTMVLKPSELAPFDAMLFAEILHEA"
BASE COUNT          402 a          687 c          700 g          401 t
ORIGIN      
        1 cctgccaggc tccatgccct gtaaatctta cacgcggttg aaatgagcgc tcaagcggtg
       61 tcgggcggcc tgttccctca gccggccttg cggacttcca gggccaggcc gaggatgacg
      121 ttgccgacga tcccgagcgc gtcgtccagc agccggtcgc tgatggtgag tggcgggagg
      181 aagcggatga cattgcctcg cgcgccgcag gacaggagga taaccccctg tttcatcgcc
      241 tccgcgacga ccgctttggt gagctcgggg tccggcctgg aggcgtcgcc gtcgcgaacc
      301 agttcaatgg cggacatcgc gccgacggtc ctgacgtcgc cgatgctgct gttcttgccc
      361 tgcagggcag tgccccagta aacaatgcgc cggccgatcg catccgcacg ctcgcagagg
      421 ccttcttccg caatgatgtc gaggacggcg agaccggcgg cacaaccgac cggcggtccg
      481 ccgtaggttg cgccaagacc gccgggaggt accgaatcca tcacctccgc cttgccgatc
      541 actccggcta tgggaaatcc gccgcccagg ctctttgcca ccgtgacgag atcggcctcg
      601 atgccggcgt gttcgatggc gaaaatcttc ccggtcctgc cgaagccggt ctggacttcg
      661 tccgcgacca gcatgatgcc gtgctcgtcg cagatggttc gcagcgagcg aaggaattcc
      721 gccggcgcag catagaagcc gccctcgccc tgcaccggtt cgatgatcag tgcggcaatt
      781 cgcgagggtt cgatatcgga gcggaacagg tcttcgagcg cgcgcaggga ttgcgcaggc
      841 gtcgtgccgt gataggcaac cgggtacggg atgtgataga cgtcgccggg aaagggcccg
      901 aagccagctt tgtatggcgc gactttaccg gtgagggcca tgcccatcat cgtgcgtccg
      961 tggaacgcgc cggagaacga aacgacgcca ctgcgccgcg tgtggtactt ggcgatcttc
     1021 acggcattct cgatcgcctc cgcaccggtg gtgacgaaca gggtcttctt cggcgtcttg
     1081 ccgggcacca gcgcgttgag tcgctccgcg agctttacgt aaggctcata aggcgtcacc
     1141 tggaagcagg tgtgcgtgaa ctgctcgagt tgagcatgca cggcggccag gactctggga
     1201 tggttgtggc ctacgttcag cacggcgatg ccggcggcca ggtctatgta gcgcctgcct
     1261 tccacgtccc acagttctgc gttcttagaa cgagctgcaa aaaccctgtg caaattactg
     1321 acgcccctag gaattgcaga gtcgcgccgc gcacttaatt gctggttggt cgtcatgtcg
     1381 gcctgccgtc acggtttaaa aaacgcgcca atgctagcac gttccccctg ccgttcagcg
     1441 agccctgcag atggtggagc gagcctgcgt aggagcccgc cgtgcggggc gagtgtgatt
     1501 cgtgttcagc gaactcgccc gcatggcggg ctcctacaac ctctcgatca tcgagcagcg
     1561 ccggcaccgg cgccgctcgc catgcgttat cattcgcggt tcgtcaggcg aggagccact
     1621 cacatgcggg acatgctgca gttctatatc gacggccgct gggtcgaccc ggtcacgccg
     1681 aaaaccgtca atgtcataaa tccggcgacc gaggaagttg tcggccgcat cagcatgggc
     1741 tccgccgaag acgtggagcg cgcggttgcg gcggcaaaag cggccttccc cgcctacagc
     1801 accacgaccc gtgacgaacg caccgcgctc ctcgaaaaaa ttttggaggt cttcgcgaaa
     1861 cgccatgacg aggttgctac cgcgatcatg gaagagatgg gcgcgccgtg gaagcttgcg
     1921 aagcacgcgc aggctgcaag cggtccgcag catatccagg cggctataga ggttcttcgg
     1981 acgttcgact tcgaagagac gctcggcacg acgcgtgtgc tgaaggaggc gattggcccc
     2041 tgcgcactga ttactccatg gaactggccg atgaaccagg ttgccgtgaa agtggcccct
     2101 gcgcttgcag ccggatgcac catggtcctc aagccgagcg aactcgcgcc attcgacgcg
     2161 atgctattcg ccgagatcct gcacgaggcc
//
LOCUS       DASUOM010000266         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_696474,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000266 DASUOM010000000
VERSION     DASUOM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696474"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFY27_05555"
     CDS             complement(<1..999)
                     /locus_tag="VFY27_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765013.1"
                     /translation="MGSYLRELQRRNVFRAIAAYVVLGWVLLQVSSTLEEALQLPDWF
                     DALVTAFLVIGFPLALVFSWVYELTPDGVRKTSSVAEGDSVAQATGRKLDYITIAGIV
                     LLITVILLDRLALPDPGEENPTASAAAEPVVLAADEKSIAVLPFVAMTASREDEFFAD
                     GLSEELLNVLSKIDGLKVPGRTSAFYYKGRNEDLRVIADALGVAHVLEGSVRRSGNQL
                     RVTAQLIKAEDGFHLWSETYDRADGDTFVIQDEIASSVANALQTRILGTSMAATATEN
                     RNVEAQNLYLVAQAAMAQRSLPDNRRARDLYAQASALDPNNPRYLAGYAIAVALQFW"
     gene            complement(1195..1608)
                     /locus_tag="VFY27_05560"
     CDS             complement(1195..1608)
                     /locus_tag="VFY27_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765014.1"
                     /translation="MIYRGSCHCGAISAEYETDESVRLRQDGCGFCSSRGVKSASDPN
                     GRLALVSQLRLIRYRFGHKTADFLVCPTCGTYVAAQMEGPRSPVGVLNVVGLAIPELK
                     DESATLASLEGESVDERIQRRLSRWTPMALEEPAP"
     gene            1971..>2435
                     /locus_tag="VFY27_05565"
     CDS             1971..>2435
                     /locus_tag="VFY27_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765015.1"
                     /translation="MKKPITTTWAVAGLLLAAGSGQAQVSAEGMGKVLPVELYLCAYN
                     DGQGPADLDKVITRWSKFMDDNNIDDYAAWTLTPVFYGPEQDFDVIWLGAYQDGNAMG
                     KGMDTWLGSGGELQAAFDKVVDCNAHLAMASAMYKAPADGATPSSGFITMMDC"
BASE COUNT          509 a          717 c          734 g          475 t
ORIGIN      
        1 ccagaactgg agggcgacgg cgatggcgta tccggccagg taacgtggat tgttggggtc
       61 gagggcggac gcctgcgcgt acagatcgcg agcccgacgg ttatccggta gtgaacgctg
      121 cgccatggcc gcctgcgcga ccaggtacag gttttgcgcc tccacatttc gattctcggt
      181 ggcggtggcc gccatcgacg tgccgagaat ccgggtttgc aacgcattgg cgacgctgct
      241 cgctatctcg tcctggatta caaacgtatc gccgtcggcg cgatcatagg tttcggacca
      301 caggtggaaa ccgtcctcgg ctttgatgag ctgagcggtg acccgcaact ggttgccgga
      361 gcggcgcacg ctgccttcca gcacgtgagc gacgccgagt gcatcggcga tcacgcgcag
      421 atcctcgtta cgccccttgt agtagaaggc cgaagtgcgg cccgggactt tcaggccgtc
      481 gattttcgag agaacgttga gcagttcctc agacagtccg tccgcgaaaa actcgtcctc
      541 tcgcgaggcg gtcatggcga caaaagggag gacggcgatt gacttctcgt cggcagcgag
      601 gaccaccggt tccgcggctg ccgaggcagt cggattctcc tcgccggggt cgggcagagc
      661 cagccgatcc aacaggatca ctgtgatcag aaggacgatt ccggcgatag tgatgtaatc
      721 gagtttgcga ccggtcgcct gcgcgacgct gtcgccttcc gcaacgctcg aagtcttcct
      781 aacgccgtcc ggcgtgagct cgtacaccca cgagaacacc agggcgagcg gaaatccaat
      841 gaccaggaat gccgtgacca gtgcatcgaa ccagtccggc agctgtaacg cttcttccag
      901 cgtgctggag acctgcagga gaacccagcc gagcacgaca tacgccgcga tggcgcggaa
      961 cacattgcgc cgttgcagtt cccgaagata gctccccatt tactcttatg cccctgtttt
     1021 tgcgccattc tacgggcttt tacctgcaag ggatacgagg gcaccgggtc cgcggaagcc
     1081 ccgatgggct gctcacttcg agatttccga tggacatttt gaaagcgcgt cgtacgggaa
     1141 ccggccaacc atcctcgttg gaggatccag actaacgcgc ttcaagttgc tccatcatgg
     1201 cgcaggctcc tccagagcca tcggtgtcca gcgtgaaaga cggcgttgga tgcgctcgtc
     1261 gacactctcg ccctcaaggc tggcaagcgt cgcggattcg tccttaagtt ccggaatcgc
     1321 aagacccacg acattgagca cgccgaccgg actacgtggg ccctccatct gcgcggctac
     1381 gtacgttccg catgtcgggc aaacaagaaa atccgcggtt ttgtgcccga accgatagcg
     1441 aatgagcctg agctgcgaga cgagagcgag ccgcccgttc gggtcgctcg cggatttcac
     1501 gccgcgactt gaacagaagc cgcagccgtc ctgcctgaga cgtacggact cgtccgtctc
     1561 atactccgcg ctgatggcgc cacaatggca tgagccgcga tagatcatga gcgcaccaat
     1621 ccgatcatga gacggcgatt gcaaccgctg tgaggcaatt cctgctcgac tggagatttg
     1681 agatagtcgc agtgtatggt ccgattcggg tcagtccctg cccgtcacaa tcatagcatt
     1741 cgacggatcg ctgtcagcgc aaaagcggtt ccgctgcatc aatacccgat aggtcggctt
     1801 aaccggggcc cccaccagtc agcatatcct gtgtacgtgg cgaaagatca tgcagggcaa
     1861 gcggtgtcgg gagcgaagtt ttcctctcgt ctcccgacga aaggtctata tttgtggtac
     1921 cggcctttcc atacggccgc aacatcataa tcacaaaaaa ggggaacgcc atgaagaaac
     1981 ctataacgac cacatgggcg gtcgccggcc tgctgcttgc ggcaggatcc ggtcaagcgc
     2041 aggtcagtgc cgagggtatg ggcaaagtgc ttcctgtgga gctttatcta tgcgcataca
     2101 acgacggcca aggtccagcc gacctcgata aggtaattac tcgctggtca aagttcatgg
     2161 atgacaacaa catcgacgac tacgccgcgt ggacactgac gccggtcttt tacggacccg
     2221 aacaggactt cgacgttatc tggctgggcg cctaccagga cggtaacgcg atggggaagg
     2281 gcatggatac atggcttggt tcaggcggtg aattgcaggc ggccttcgac aaagtagtcg
     2341 actgcaatgc gcatctcgcg atggcgtcgg cgatgtacaa ggcgccggcg gatggtgcta
     2401 ccccgtcatc cggcttcata acgatgatgg attgc
//
LOCUS       DASUOM010000267         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_851770,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000267 DASUOM010000000
VERSION     DASUOM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851770"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFY27_05570"
     CDS             <1..465
                     /locus_tag="VFY27_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5765016.1"
                     /translation="MTSPCGKIRIPLNESQDDKSQIEEYLRDYKGEGIQHIALGTDDI
                     YRTVDRLTANGVELQDTNDAYYEMVDERVPGHGEPLEELKKRHILIDGAPQKDQGLLL
                     QIFTRNVIGPIFFEIIQRKGNEGFGEGNFKALFESLEADQIRRGVLAPDASK"
     gene            487..867
                     /locus_tag="VFY27_05575"
     CDS             487..867
                     /locus_tag="VFY27_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765017.1"
                     /translation="MKLRIQDNSIRLRLTRSEVDELSADGSVTASVSFPDGATLEYSL
                     ETSSLTGQPRAQFSSDRLVVQIPEAVARRWAVTEEVSITGGQPLEDGALSILVEKDFA
                     CLTPREGEDDSDMFPHPLKGQENC"
     gene            996..2060
                     /locus_tag="VFY27_05580"
     CDS             996..2060
                     /locus_tag="VFY27_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008218385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX5765018.1"
                     /translation="MAVFEDPAFDDHERVIFCRDAATGLKAIIAIHSTALGPAAGGCR
                     LWQYSSDDAALHDVLRLSQGMSYKNAMAGLSFGGGKAVILKSADFAGTDALFEKFGDF
                     VEQLDGSYITAEDVGMSVAIMQTIARRTRFVTGLPKKAGHAGGDPSPITAYGVFKGIE
                     AAVHFKLGRDSVKGLRVAVQGVGNVGYNLCRYLAEAGARLLVADINEARVQSAREEFG
                     AEAVALDDILFQKVDVVSPCALGAVIDEQAIPRLQAPIVAGAANNQLETLADGQRLSD
                     AGILYAPDYVINGGGIINVASEYHGTESDAEVMQLVEAIGPRLTRIFETAASTGRPTN
                     VIADEQARKIIADAQARKRT"
     gene            complement(2170..>2392)
                     /locus_tag="VFY27_05585"
     CDS             complement(2170..>2392)
                     /locus_tag="VFY27_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765019.1"
                     /translation="DFWLDDSLEAAEKLGLLHRSAVPNSFRTEYGSDTVWPTAIVVDR
                     LGIIRFTELSKHISDRPDPEALINVLRQM"
BASE COUNT          521 a          694 c          744 g          433 t
ORIGIN      
        1 atgaccagtc cctgcgggaa aatccgcatt ccgctgaacg agagccagga cgacaagagc
       61 cagatcgagg aatatttgcg cgactataag ggtgaaggca tacagcatat cgccctgggc
      121 accgatgata tctaccgcac cgtggaccgg ctgaccgcca acggggtcga gttgcaggac
      181 acgaacgacg cctactatga gatggtggac gagcgtgtcc cgggacatgg ggagccgctc
      241 gaggaactga aaaagcggca catcctcatc gacggagcgc cgcagaagga ccagggcctg
      301 ctgctgcaaa ttttcacccg taatgtcatc gggccgatat tcttcgagat cattcagcgc
      361 aagggcaacg aaggcttcgg cgagggcaat ttcaaggcct tgttcgagtc gctcgaggcg
      421 gaccagatcc gccgcggagt gttggcgccc gacgcatcga aatagccgtg caacaggaga
      481 aagtgcttga agctccgcat acaggacaat tcaatacgct tgcgcctgac ccgttcggaa
      541 gtggatgaac tcagtgcaga cggtagcgtc accgcctccg tttccttccc ggacggcgcg
      601 acgctcgaat attctctcga aacctcttcg ttgacgggac agcccagggc gcagttctcg
      661 tcggatcgcc ttgtcgtaca aatcccggag gccgttgcgc gcagatgggc tgtgaccgag
      721 gaggtctcca ttaccggcgg acaacctctc gaagacggcg cgttatcgat actcgtcgaa
      781 aaggactttg cctgcctcac cccgagggaa ggagaagacg actccgacat gttcccgcac
      841 ccgctgaagg gccaggaaaa ctgctagctg gtttgtgacc tgccgcattg gcggtgctag
      901 aatccgcgcg ccacgaccgg atggcctggt cgaaaagtcg atgcaaggcc catcttccgg
      961 aaattcctca gtcaccttcc atatcgggac aggccatggc cgtttttgaa gatccagcat
     1021 tcgatgatca cgagcgtgtg attttctgcc gcgacgcggc gaccggcctg aaagcgatta
     1081 tcgctatcca ctcgaccgcg ttgggacctg ctgccggagg ctgcaggctc tggcagtact
     1141 cctcggacga tgctgcgctg cacgatgtgc tgcggctctc ccaggggatg agctacaaga
     1201 acgcgatggc ggggctgtca tttggcggcg gcaaggctgt aattttgaaa tcggcagatt
     1261 tcgccggcac ggatgcgctg ttcgagaagt tcggcgactt cgtcgaacag ctggatggca
     1321 gctatatcac cgccgaagat gtcggcatga gtgttgccat catgcagaca atcgcgcgac
     1381 ggacgcgatt tgttaccggc ctgccgaaga aagcaggaca tgccggcggc gatccgtcgc
     1441 caatcacggc gtacggagtg ttcaagggca tcgaggccgc ggttcacttc aagcttgggc
     1501 gggacagcgt gaaaggcctc agggtagcag tgcagggcgt cggcaacgtc ggctacaacc
     1561 tttgccgtta cctcgccgaa gccggcgcca ggctcttggt agccgacata aatgaggcgc
     1621 gcgtgcagtc tgcgcgcgaa gagtttggtg ctgaggccgt tgcactcgat gacatcctgt
     1681 tccagaaggt ggacgtggtg tcgccgtgtg cgctgggcgc ggtcattgat gaacaggcga
     1741 tcccgcggct tcaagcaccg atagtcgcgg gtgcggccaa caaccagctc gagacccttg
     1801 ccgacggcca gcgcctgtcc gacgcaggaa tcctgtatgc gccggattac gtgatcaacg
     1861 gcggcggcat aatcaacgtt gccagcgaat atcacggcac ggaaagcgac gcggaagtaa
     1921 tgcaactggt cgaggcgatc gggccgaggc tcacccgcat cttcgaaacg gcggcatcga
     1981 cgggacgtcc aacgaacgtg atcgcggatg agcaggcgcg caagatcatc gctgacgcgc
     2041 aggcccgcaa acgaacgtag gagccacgcc gaaaggaatc gacacgatat agatccgggt
     2101 tgctttgcgg acggagaatg cggagcgagt tcgctaacgc tcacgggaac tcgcccgcat
     2161 ggcggcctcc tacatttggc gtaatacgtt aatcagcgcc tccggatccg gccggtccga
     2221 gatatgcttc gacaactccg taaagcgaat gatgcccaac cggtctacca cgatggccgt
     2281 cggccacacg gtatcgctgc cgtactcggt gcggaacgaa ttcggcactg ctgaccggtg
     2341 cagcaaacca agcttctcgg ccgcctccag cgagtcgtcc agccagaagt cg
//
LOCUS       DASUOM010000268         8348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1339700,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000268 DASUOM010000000
VERSION     DASUOM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339700"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..513
                     /locus_tag="VFY27_05590"
     CDS             55..513
                     /locus_tag="VFY27_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5765020.1"
                     /translation="MISEFRKRRLRDRATGPATLISEGCKITGILSGQGDFMISGTVE
                     GDCDLSGSVSITRQGVWNGTLKAASVIIAGTVEGDIEATGRVEITDSARITGTVTGEA
                     IAVAEGAVVEGIMRTTGRSKPTGFVEKRRPEDTGDDDQARGSRQEPEEPL"
     gene            complement(523..1848)
                     /locus_tag="VFY27_05595"
     CDS             complement(523..1848)
                     /locus_tag="VFY27_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765021.1"
                     /translation="MRKSALIVDDSKSARVVLKRMLEVHELDVDTAESAESALEYLND
                     HRPDVIFMDHMMPGMDGFEAVTAIKNNPDTATIPIMMYTSQQGELYVGQARALGAVGV
                     LPKAVEPVEVSKVLEALHVIGERVEREKQALRSDPQGLSAATASTDSLDRGIRELIQD
                     LFDQQRAVLRRDLLDSYEAIAARVADEIRVPDSEDQAEPKKDRGHEMPPRFQLIVAVM
                     AVVIVVFAWLYWQREQGWQQARQQNATLLAALEQQRSIEADGAIEVQRRLTTYQQTLG
                     STFSTALDAVEWAVNRSGGYSFDELPLGDERLALVEELSSRLIGLGFSGEVLVETHVG
                     DFCMVDGGADGLELADPRMRVSDCTNIGFESSEAEQRGLRQSVAFANFVNVAEERTDG
                     QIRYRFISRGNSQPLLPYPATMDGPAASWNEVALANNRVQITLMPDGFQ"
     gene            2333..4765
                     /locus_tag="VFY27_05600"
     CDS             2333..4765
                     /locus_tag="VFY27_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765022.1"
                     /translation="MPRYLIVASTLALVVVGIMLTMFYGQYRWLADGIVDAGVREHNL
                     SLEASFERRSRGTLHRIADELASLPDGADEVAALAIIEDAVRSNDDLVGLRFERVDGV
                     NIQTGEVTEMSGAETQWEANRVYVTYPVLSDERQLGTLVSSFELSALREESALFEGRL
                     QAEEARHRRDSFAWIAGATGFALLLCLAVIWGVSRSQARRIRELRVQAEKLSDADFGE
                     PIKVMKGDELGDLAEVFNSMREKLQRTTLSRDYVDRVLSSMNEAVIVTNADGIITRVN
                     EATLRMLSYSEDELVGQPIDFIVDQQKSGALVIDSPAGMPREAFLLSCSGESVPVSYT
                     GSTIEGDDVSPGDRIYAAQNITERRRAEQRIRYLARIDALTKVPNRMQFQHLLQRGIA
                     RARRARKSLYLFYIDIDHFKEINDTFGHLAGDTTLETVADRLTTALPSDSIIGRLAGD
                     EFAVIVDGNDARDDRRQQLDELARTLLDRLADPFYVQGHEVFMTASMGIARYPDDAPN
                     VIDLIRNADAALYHAKKSGGNIHAYYVPEMNEAAVERLMTKSKLKRSFERDELLVHYQ
                     PKYNIVTGEVVGAEALVRWELPERGIILPSDFIPIAEETNLIVEIGEWVLDRVCEDFR
                     YWQRSVSYPGRVSVNLSLKQLRQPNFISRINAILRKYEVSPTSLELEITETTLMENPS
                     RTIKLLDELYSLGLHLAIDDFGTGYSSLSALQQFPISTLKIDQSFVRDVATNADDATI
                     VGTIIHMGRSLKMDVVAEGVETEDQLNFLQTLDCTYVQGLLFGDPMSSDNYLELLLAQ
                     AGGTDTHRALFA"
     gene            4916..5275
                     /locus_tag="VFY27_05605"
     CDS             4916..5275
                     /locus_tag="VFY27_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765023.1"
                     /translation="MRKLVKFACCTSLLVATQVTLACDYPTKASIPNGSTASQEEMIE
                     GQRGVKKYVADMETYLACIVEEEKAARAEIEDLQPEVEQQREEMLTKKYNAAVEEMEQ
                     VAAKFNVEVQTYKARND"
     gene            complement(5416..5634)
                     /locus_tag="VFY27_05610"
     CDS             complement(5416..5634)
                     /locus_tag="VFY27_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765024.1"
                     /translation="MNVFTFAFLTITVVLFAKLIHAWIENRSKLNSESEAVNETLLKI
                     DQLEERIQVLERIITEDRYDLKKRIDNL"
     gene            complement(5657..6043)
                     /gene="pspC"
                     /locus_tag="VFY27_05615"
     CDS             complement(5657..6043)
                     /gene="pspC"
                     /locus_tag="VFY27_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HEX5765025.1"
                     /translation="MSDYSSIRTRRFYRNRDRAIIAGVCAGFADYFGFNLKVTRILVV
                     IAFFMAMPVTLLVYFATVFLVPSAPDPGRKADYDPEFRRALRSSPSQTLGDVRRRFQS
                     LDSRLARLERYVTSSRFNLDQEFRKL"
     gene            complement(6040..6330)
                     /locus_tag="VFY27_05620"
     CDS             complement(6040..6330)
                     /locus_tag="VFY27_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX5765026.1"
                     /translation="MTKETLGPGIASGLYRDRENGWIFGVCAGVADYASIPVAVVRVI
                     TAAGLLMFFWATVVTYGAATLLLREKPLTWSGHCQEHEFWCRRRSDDRWSHS"
     gene            complement(6327..6551)
                     /gene="pspB"
                     /locus_tag="VFY27_05625"
     CDS             complement(6327..6551)
                     /gene="pspB"
                     /locus_tag="VFY27_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEX5765027.1"
                     /translation="MSEYIGIALIVFIAVPAPLFIILYFITEWKRSREISGGDEKLLE
                     DLWLLSQRLEERMESLETILDSEMPEWRRK"
     gene            complement(6574..7263)
                     /gene="pspA"
                     /locus_tag="VFY27_05630"
     CDS             complement(6574..7263)
                     /gene="pspA"
                     /locus_tag="VFY27_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEX5765028.1"
                     /translation="MGIFTRFSDIVNSNINAILDKAEDPEKIVRLMIQEMEDTLVEVR
                     SAAARSIADKKDLNRKLESVEREQEEWDSKAELAMRKGREDLAKAALIEKSRTTAAAE
                     AIKADYSAVDESLTKLNDDIARLESKLQDAKARQKSLLARHKTANSRLAARKKIHDYK
                     IDDALVRFEQYTRRIDDVEGRVEAYDLGLPKDLKHEFVSLEAEEAVTRELDELKARVA
                     GRPAGQATEPK"
     gene            7458..>8348
                     /locus_tag="VFY27_05635"
     CDS             7458..>8348
                     /locus_tag="VFY27_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5765029.1"
                     /translation="MSRDRPVQPIIGEAPAFLEMLEHVSRAAPLAKPVLVVGERGTGK
                     ELIASRLHFLSSRWEQPLVKVNCAALTETILESELFGHEAGAFTGAAKTHVGHFERAD
                     GGTLVLDELATISLRMQEKILRVIEYGELQRVGGSSTIQVDVRIVGATNADLQELAAG
                     GRFREDLLDRLAFDVITVPPLRERLEDILPLSYAFAVNMVSELKRQYFPGFSPRAASS
                     LLRHDWPGNVRELKNAIERSIYRSTEADQQVKHIAFDPFDSPFRLRERKDTGANQAPA
                     PAAAQKRTAKLPLDLKSHLNE"
BASE COUNT         1715 a         2509 c         2451 g         1673 t
ORIGIN      
        1 attagccccg gtgctaccgc cggcggcggg caatccctgt gatggagagt ccagttgata
       61 tcggaattca gaaaaagacg ccttcgcgac cgcgccaccg gcccggcgac gctgatcagt
      121 gaaggctgca agatcaccgg gattctgtcg ggccagggcg atttcatgat cagcggcacc
      181 gtcgagggcg actgtgacct gagcggctca gtgtcgataa cgaggcaggg cgtctggaac
      241 gggacgctca aagctgcctc ggtaatcatc gcaggcacgg tcgagggcga tatcgaagca
      301 acgggtcgtg tggagataac ggactcggcg cgcatcaccg gcacagtcac cggcgaagca
      361 atcgccgttg cggaaggagc cgtggtggaa ggaatcatga ggactaccgg acgcagcaag
      421 cccaccggtt ttgtcgagaa gagaaggccg gaagataccg gcgacgacga tcaggccagg
      481 ggatcccggc aagagcctga ggaaccgctg tagccccaag cgctactgga agccgtctgg
      541 catcagcgtt atctgcacgc ggttattggc gagggcgact tcgttccagc ttgctgccgg
      601 gccatccatc gtggctgggt acggcagaag cggctgtgaa ttgccgcgcg agataaaccg
      661 gtagcgaatc tgcccgtcgg ttcgttcctc ggccacgttt acgaaattgg caaaggctac
      721 tgactggcgc agtccccgtt gttctgcttc gctgctttcg aagccgatgt tcgtgcagtc
      781 cgaaacccgc attcgcggat cggcgagttc caggccatcc gcgccaccgt ccaccatgca
      841 gaaatcgccg acgtgggttt cgaccagcac ctcgcccgaa aagcccagcc cgatcaacct
      901 cgaggaaagt tcctcgacca gggcgaggcg ctcgtccccc agcggcaatt catcgaaact
      961 gtaaccgccg gacctgttga cggcccattc gacggcatcc agcgcagtag agaacgtgga
     1021 gcccagcgtc tgctggtagg tggtgagccg tcgttgcact tctatcgcgc catcggcctc
     1081 gatggaacgc tgttgctcca gcgccgccag caaagtcgcg ttctgctgcc gcgcctgctg
     1141 ccatccctgt tcacgctgcc agtagagcca cgcgaaaacc acgatgacca ccgccatgac
     1201 agcgacgatc aactggaagc gtggcggcat ctcgtggccg cggtccttct tcggttctgc
     1261 ctggtcttcc gaatcgggta cacggatttc atcggcgacg cgggcggcga tcgcttcata
     1321 gctgtccagg aggtcccggc gtagcactgc ccgttgctgg tcgaagagat cctggatcag
     1381 ctcgcgtata ccccggtcga gagaatcggt ggaagctgtg gcggcggaaa ggccctgcgg
     1441 atcggaccgc agcgcctgct tctcccgttc gacccgctcg ccgatgacgt gcagcgcttc
     1501 gagaaccttc gagacttcca ctggttcgac cgccttgggc agcaccccca cggcacccag
     1561 cgcccgcgcc tgccccacat aaagctctcc ctgctgggag gtgtacatca tgatcgggat
     1621 ggtcgcggtg tccggattgt tcttgatcgc cgtgacggcc tcgaagccgt ccattcccgg
     1681 catcatgtga tccatgaaaa tcacgtctgg acggtggtcg ttcaggtatt cgagcgcgga
     1741 ctccgcggac tcggcggtgt cgacgtccag ctcgtgcacc tcgagcatac gtttcagcac
     1801 gacccttgca gatttcgagt cgtcgacgat caacgcgctc tttctcacgt cacttatacc
     1861 ccgggatttg cccaagccga ctttgattat gttacgcaaa gaatacccgt acttggttca
     1921 cgtttggccc agcgaactgg tgccatgcca tcgacgggaa gccggaaggc agcctttctc
     1981 actgcgctac cgtgcattag tacgtatctg gtcgcaaaac tccacgtatt tcgcggaaat
     2041 gggctctttc cggtacctgc gcgtccgttt taaggtgacc cctgcggctg gtctgcgccg
     2101 gttgtgtgcc ggactgggag ggaaccgcct gttaccgcta tcgaaaactt gcgggagcaa
     2161 aatgacaggg tgttaaacta ccgccgacct cgaaatcagg gcggaggcgg cgctcaagcc
     2221 aggccggcaa gtgcgcagcc tgaagaataa gaaccaaaaa cgagaaggac caccacggat
     2281 cactgaatcc gtcccggacg tgagagtctc acacaagatg gcccgcagaa tattgcccag
     2341 atacctgatc gtcgcctcta cgctggcgct cgtcgtcgta ggcattatgt tgacgatgtt
     2401 ttatgggcag tatcggtggc ttgcggatgg catcgtggat gcgggcgtcc gggagcacaa
     2461 cctgagtctc gaagcgagct tcgagcgccg gtcgcggggg acgttgcatc gtatcgcgga
     2521 cgaactggcg tcgctgccgg acggagcaga cgaggtcgct gctctggcca tcatcgagga
     2581 tgctgtccgc agcaacgatg acctggtcgg gctgcgcttc gagcgggtcg acggtgtgaa
     2641 catacaaacc ggtgaagtga cggagatgtc cggcgccgag acacagtggg aagcgaaccg
     2701 ggtctacgtc acctacccgg ttttgagcga cgagcgacaa ctcggcacgc tcgtcagcag
     2761 ctttgaactc tcggcgctgc gtgaggaatc cgcgttgttt gaaggccgtc tgcaggcaga
     2821 ggaagcgcgg catcgccgcg acagttttgc ctggatcgcg ggcgccactg gtttcgcact
     2881 cctgctttgc ctggccgtga tctggggcgt gtcccgttcc caggcacgtc gcatccggga
     2941 gcttcgggtc caggcagaga agttgagcga tgccgatttt ggcgagccga tcaaggtcat
     3001 gaagggtgat gagctcggtg atctcgctga agtgttcaac agcatgcggg agaagctgca
     3061 gcgcactacg ctgtcccggg actacgtgga tcgggtgctg tcgagcatga acgaggccgt
     3121 catcgtaacg aatgccgacg gcatcatcac gcgtgtcaac gaagcaacat tgcgtatgct
     3181 cagttacagc gaggatgagc tggtcggcca gccgatcgac tttatcgtgg atcaacagaa
     3241 gagcggcgcg ctggtgatcg attcgcccgc gggcatgcca cgagaagcgt ttctcctgtc
     3301 ctgcagcggg gaatcggtgc cggtgtcata caccgggtcg accatcgaag gcgatgacgt
     3361 atctcccggc gaccgtatct atgccgccca gaacatcacg gaacgccgac gtgcagaaca
     3421 gaggatccgc tatctcgcgc gcatcgacgc actgacgaaa gtgccgaacc gcatgcaatt
     3481 ccagcacctc ctgcagcgcg gcattgcccg ggcgaggcgc gcccgcaaat cgctgtacct
     3541 cttctacatc gacatcgatc atttcaagga aatcaacgat accttcggtc accttgccgg
     3601 cgacaccacc ctcgagaccg tcgcggaccg cctgaccacg gcgctgccct ccgattcgat
     3661 tatcgggcgc ctcgcaggcg acgagtttgc tgtaatcgtc gacggcaacg acgcacgcga
     3721 cgaccgccgg caacagctgg acgagctggc gaggacgttg ctggaccggc tcgccgatcc
     3781 cttctacgtg cagggtcatg aagtgttcat gaccgcgagc atgggtatcg cccggtatcc
     3841 ggacgacgcg ccgaacgtga ttgatctgat tcgtaacgcg gatgcagcgc tctaccatgc
     3901 gaagaaaagc ggcggcaaca tccatgcgta ctacgtcccg gagatgaacg aggctgccgt
     3961 cgaacggctg atgaccaaga gcaagctgaa gcgctcgttc gaacgcgacg agctgctcgt
     4021 acattaccag ccgaagtaca acatcgtcac cggtgaggtc gtcggggcgg aagcgctggt
     4081 gcgttgggag ctgccggaga gaggcattat cctgccctcc gatttcatac cgatcgccga
     4141 ggaaacaaac ctgatcgtcg agatcggcga atgggtactc gaccgggtgt gcgaggattt
     4201 ccggtactgg caacgttccg tttcgtaccc cggccgcgtc tcggtcaacc tgtcgctcaa
     4261 gcagttgcgg caaccgaact ttatcagccg catcaatgcg atcctgcgca agtacgaagt
     4321 atcgccgacg tcgctggaac tcgaaattac cgaaacgacc ctgatggaga accccagccg
     4381 cacaatcaag ctcctggatg agctttactc actgggcctg cacctggcga tcgacgactt
     4441 cggcaccggc tattcttccc tcagcgcgtt gcagcagttc ccgatcagca ccctcaagat
     4501 cgaccagtcg ttcgtacgtg acgtcgccac aaacgctgac gatgcaacca tcgtcggtac
     4561 gatcatccac atggggcgga gcctgaaaat ggatgtggtc gcggagggcg tggagacaga
     4621 agaccagctc aactttctcc agaccctgga ctgcacctat gtgcagggac ttcttttcgg
     4681 tgatccgatg agctccgata actatctgga gctgttgctc gcgcaggccg gaggcacgga
     4741 tacgcatcgc gccttgttcg cctaattctc ctggcccgtt tctcgctaaa gcgctgacat
     4801 cacatgacat tttcctgtgc ttcactcgtc gcaggcaaca tgcttaaata atgccagttc
     4861 gccgaggcgc ggaatcatcc gatccttctt cctaatcaga taacggactt catcaatgag
     4921 aaaactcgtc aagtttgcat gctgtacctc gcttcttgtt gcgacacagg taacccttgc
     4981 ctgcgactac ccgaccaagg catcgatacc gaacggcagt acggcgagcc aggaagagat
     5041 gatcgagggc cagcggggcg tcaagaagta cgtggcggac atggaaacct acctcgcgtg
     5101 tatcgtcgag gaagagaaag ctgcacgcgc ggaaatcgaa gatctccaac cggaagtcga
     5161 gcagcaacgg gaagagatgc taaccaagaa atacaacgct gccgtggagg agatggaaca
     5221 ggttgcggcg aagttcaacg tcgaggtgca aacgtacaaa gcccgcaacg actgattccc
     5281 gctcgctccc gaattcgcta ggcaggggag cctgcaatgc accaggggaa atggaggagt
     5341 gctcttccgt ttcccctttt tctgcctccg ccactagcct ctgcatcgtg actcgcgcag
     5401 aggctcgcca ggacactaga ggttgtcgat cctttttttc aggtcgtagc ggtcttccgt
     5461 gataatccgc tccagcacct ggattcgctc ctccagctgg tcaatcttga gtaacgtctc
     5521 atttaccgct tcgctttctg aattcagctt gctgcggttc tcgatccagg catggatcag
     5581 tttcgcaaaa agtacgacgg tgatcgtcag aaaggcaaac gtaaagacgt tcatctaagg
     5641 ttccttatgc ggcttgtcag agtttcctga attcctggtc caggttgaac ctggacgacg
     5701 tcacgtagcg ttccagccgc gcgagacggc tgtcgagcga ctgaaagcgc cggcggacgt
     5761 cccccagcgt ctggctgggc gacgatctca aagcgcgccg aaactccgga tcgtaatcgg
     5821 cctttcttcc cgggtcgggc gcggatggca cgaggaacac tgtggcgaag tacacaagca
     5881 gggtcaccgg catcgccatg aagaaagcta tcactacaag gatccgcgtg accttgaggt
     5941 tgaagccaaa gtagtccgca aagccggcac agacgccggc aatgatcgcg cggtcgcggt
     6001 tccggtagaa acgtcgcgtc cggatggaac tgtagtcgct catgaatgac tccaacgatc
     6061 gtcggacctg cggcggcacc agaactcgtg ttcctggcag tgaccggacc aggtcagcgg
     6121 tttttcccgc aacagcagcg tcgccgcgcc atacgtcacc accgttgccc agaagaacat
     6181 cagcaatccg gccgctgtta tgactcgtac gacggccacg ggaatgcttg cgtagtcggc
     6241 aacgcctgcg cacacgccga atatccagcc gttctcccga tcccggtaga gcccggaagc
     6301 aatcccgggg cccaaggttt cctttgtcat ttccgcctcc actccggcat ttcgctgtcc
     6361 agaattgttt ccagcgactc cattcgttct tccagtctct gggacaacaa ccacaggtcc
     6421 tcgagcagct tttcgtcacc tcccgaaatc tcccgggacc gcttccactc ggtaatgaaa
     6481 tacagaatga tgaaaagcgg ggccgggaca gcgataaaga cgattaacgc aatgccaatg
     6541 tattcgctca atttcgcccc tcgttaattt ctgttatttg ggttctgtcg cctgccccgc
     6601 gggtcgtccg gccacccttg ccttgagttc gtccagctcc cgtgtaaccg cttcctcggc
     6661 ttccaggctg acgaactcgt gtttcaggtc tttcggcaag cccagatcgt acgcctcgac
     6721 acgcccctcc acgtcatcga tgcgccgcgt gtactgctcg aaccgcacaa gcgcatcgtc
     6781 gatcttgtag tcgtggatct tcttcctcgc agccagccgg ctgttggcgg tcttgtgccg
     6841 cgccagcagg gatttctgcc gcgcctttgc gtcctggagc ttgctttcga gccgcgcgat
     6901 gtcgtcgttc agcttcgtca ggctttcatc gacagcactg tagtccgcct tgatggcttc
     6961 cgcggccgcc gtggtacggg atttttcgat cagcgccgct ttggccagat cttcgcggcc
     7021 cttgcgcatc gcaagctcgg ctttcgagtc ccattcctcc tgctcgcgtt cgacggactc
     7081 gagcttgcgg ttcaggtcct tcttgtccgc aatcgaacgt gccgcggcgg agcgtacttc
     7141 gaccagcgtg tcttccattt cctggatcat caggcgaacg atcttttccg gatcctcagc
     7201 cttgtcgagg atggcgttga tattcgaatt cacgatatcg ctgaatctcg tgaaaattcc
     7261 cataggtaaa tcctctgttg tctggtaagg gtcggtcgga atgcttattg cagggaccgt
     7321 gccaagccac caaaaggcaa caaccgactg ttttcaaaga ggtttcagct ctgctgccgg
     7381 cagctgaggt gccaggttat gtggcgcgaa tagccccgag ttggcttctt tgaccagtaa
     7441 atggtcaaat aaaccaaatg agcagagacc ggcccgtaca acccatcatc ggcgaagcac
     7501 cggccttcct ggaaatgctg gagcacgtgt cccgcgcggc gcccctcgcc aagccggtgc
     7561 tggtggtggg agaacgcggt accggaaagg aattgatcgc ttcccggctg cacttcctct
     7621 cctcgcgctg ggagcaaccg ctggtcaagg tcaactgcgc cgcgttgacc gaaacgatcc
     7681 tggagtccga gttgttcggc cacgaagccg gggcgtttac cggcgcggcc aagacccacg
     7741 tgggccactt cgaacgagcg gacggcggca cgctggtgct cgatgagctt gcgacaatct
     7801 ctctcaggat gcaggaaaag atcttgcggg taatcgagta cggcgagctg cagcgagtcg
     7861 gtgggagcag caccatccag gtggacgtca ggatcgtcgg cgcgaccaat gcagacttgc
     7921 aggagctggc cgccggcggc cgattccgtg aagacctgct ggacaggctc gcgttcgacg
     7981 tcattaccgt tccgccgctc agggaacgcc tggaggatat cctgccgctg tcctatgcct
     8041 tcgctgtcaa catggtaagc gaactcaaac gccagtattt tcccggcttc agcccgcgtg
     8101 ccgcgtcctc gctgctgcgc cacgactggc cgggtaacgt gcgggagctc aagaatgcga
     8161 tagaaaggtc catctatcgt tcaaccgagg cagaccagca ggttaaacac atagccttcg
     8221 acccattcga ttcccccttc aggctgcgcg aaaggaaaga cacgggggcg aatcaagcgc
     8281 cagcgccggc ggcggcgcaa aagcgaacag cgaagttacc tcttgacctg aagtcgcacc
     8341 tcaacgaa
//
LOCUS       DASUOM010000269         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_881549,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000269 DASUOM010000000
VERSION     DASUOM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881549"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFY27_05640"
     CDS             <1..1122
                     /locus_tag="VFY27_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5765030.1"
                     /translation="RLAVSGVGASLSRTAVAIVALAVAVSATIGVSIMVDSFRISVSE
                     WIDHTLRSDIYVGVARGTLDAELVGDLVQLPDIEDYSISRRVWLESPAGRTRLTAIDM
                     AREAYAGVNLEEGDPEEAWRAFDKQGAVLVSSSYAYRHAVGRGSRVKLPTPAGDRQFP
                     VAGVYRSYDADLDAVLISREAYLSIWDDRSIGSVGIYLRDDADVDGVIRDLRNISNGR
                     QALLINSNRELRERSLQIFDRTFVITGVLYWLALTVAAVGILGAMLALQLERARELAI
                     YRALGMTPAELGRMVVLQTSFIGLLSGLAAIPLGLMMAWVLVNVINRRAFGWQLDTTI
                     STGVLVEAVLLAVAAATVAGLYPAWRAAQARPALAMREE"
     gene            1124..2281
                     /locus_tag="VFY27_05645"
     CDS             1124..2281
                     /locus_tag="VFY27_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX5765031.1"
                     /translation="MKKMSLLIGFIAFIALLDGCDQEHQGARDRNSVTSSRLSELLSP
                     GDLEGYALALEPREFSFPSDHGPHPEYRNEWWYVTGNLDAAGGGRFGFELTIFRFALA
                     PPGEGQADLSSGWATNQVYIGHFALTDVAAGRFHVAERVARGALGLAGAQAEPLRVWV
                     EDWRLQEADRDADQWNIHAVDGDVELSLLLDALKPPVLNGTGGLSQKSSKRGNASYYY
                     SIPRFQTEGKLSVAGETHDVSGLAWLDREWGSSALSAGQEGWDWFALQLSDGSELMYY
                     NLRRTDGSNDAHSAGTFIPGEGDSVHLARQDVTVVVNDYWDSPGGGRYPMGWTIAVPR
                     LDLLLQVEPVLEAQELITNVRYWEGAVDVTAERAGKPVAGRGYVELTGYAR"
     gene            complement(2269..>2726)
                     /locus_tag="VFY27_05650"
     CDS             complement(2269..>2726)
                     /locus_tag="VFY27_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX5765032.1"
                     /translation="LLFAGLGSACSGYVGRLARPRNSPLTLVVSAIVATALAYLLLLP
                     FVFERFAGLPDMQKIAISLLLIAPLAFFMGMPFPLGLSRVAGEAPAFVPWAWGINGFA
                     SVVSASMATLLAIEFGFGAVVMLAMLLYGLAAAVFRAWPGGSSHRSQRA"
BASE COUNT          508 a          808 c          918 g          492 t
ORIGIN      
        1 cgcctggctg tctccggtgt aggtgcgtcg ttgagccgca cggcggtagc gatcgttgct
       61 ctggccgtcg ccgtatcggc gaccatcgga gtgagcatca tggtcgacag cttccggatc
      121 tcggtcagcg agtggatcga ccataccctg cgctccgaca tttatgtcgg cgttgcccgc
      181 ggcaccctgg atgcggagct cgtcggggac ctggtgcaat tgccggacat cgaggactac
      241 agcatcagcc gtcgcgtatg gctcgagtcc cccgcaggcc ggacgcgctt gactgcaatc
      301 gacatggcaa gagaagcata cgccggtgta aacctcgagg aaggcgatcc cgaggaggcc
      361 tggcgggcat tcgacaagca gggagcggtg ctcgtctcca gttcctacgc gtatcgtcac
      421 gccgttggca ggggcagtcg cgtgaaattg cccactcccg cgggcgacag gcaatttccg
      481 gtcgcgggtg tctacaggag ttacgacgcg gacctcgacg cggtcctgat cagccgcgag
      541 gcttatcttt ccatctggga cgatcgatcc atcggttccg tcggcattta cctgcgcgac
      601 gatgcggatg tcgacggcgt aattcgcgac ctccggaaca tcagcaacgg gcgacaggcg
      661 ctcctgatca actccaaccg ggaattgcgg gaacgttcgc tgcagatatt cgaccggaca
      721 ttcgtcatta ccggagttct ctactggctc gcattgaccg tcgccgcggt cggcattctc
      781 ggcgcgatgc tggcgctgca actcgagcgc gcgcgcgaac tggccattta ccgggcgctc
      841 ggcatgacac ctgcggaact cgggcgaatg gttgtgctgc agacgtcgtt catcgggttg
      901 ctgagcggcc tcgctgcgat cccacttggg ctgatgatgg cgtgggtgct ggtcaacgtc
      961 atcaatcggc gcgccttcgg atggcaactg gatacgacca tttcgacagg agttctcgtg
     1021 gaggctgtcc ttctcgcggt cgcggcggca accgtggctg gtctctatcc cgcctggcgg
     1081 gcggcgcagg ctcggccggc gctcgccatg cgggaggaat gacatgaaga agatgtcatt
     1141 attaattggt tttattgctt ttatcgcatt gctggatggc tgcgatcaag agcaccaagg
     1201 cgcgagagac cgaaactccg ttacctcatc gcggctgtcg gaactgttga gccccgggga
     1261 cctcgagggg tacgcgctgg cgctcgagcc gcgcgagttc tcctttccat ctgaccacgg
     1321 tccgcaccct gagtaccgca acgagtggtg gtacgtgacc ggcaatctcg atgcggcagg
     1381 cggcgggcgc ttcggtttcg agctgacgat tttccggttc gcactggcgc cgcccgggga
     1441 agggcaggcc gacctgtcgt cggggtgggc gacgaaccag gtgtacatag ggcatttcgc
     1501 gcttacggac gtcgcagccg ggcgcttcca cgttgcggaa cgggttgcgc gcggtgccct
     1561 gggtctcgcc ggcgcgcagg cagaaccgct gcgggtctgg gttgaagact ggcgcttgca
     1621 agaggccgat cgggacgcgg atcaatggaa cattcacgcg gtcgatggcg acgtcgagct
     1681 gtcgttgtta ctggacgcgc tcaagccgcc ggtattgaac gggaccggcg gcctctcaca
     1741 gaagtcgtcg aaacgcggta atgcgagtta ttactactcg ataccgcggt ttcaaaccga
     1801 aggaaagctg tcggtcgcgg gcgagactca tgatgtttcc ggcctcgcat ggctggaccg
     1861 cgaatggggc agcagtgcgc tgtctgccgg gcaggagggc tgggactggt tcgcgttgca
     1921 gctgtccgac ggcagcgaac tgatgtacta caacctgaga agaacggacg gaagcaatga
     1981 tgcccacagc gccggaacgt ttatcccggg cgaaggcgac agcgtgcacc tcgcgcgaca
     2041 ggatgtaacg gtggtcgtca acgactactg ggacagcccg ggtggcggcc gctacccgat
     2101 gggatggacg atcgccgtgc cgcgcctcga tctgctgctg caggtagaac cggtcctcga
     2161 ggcgcaggaa ctcattacga acgtgcgtta ctgggaaggt gcggtcgacg tcacggcgga
     2221 gcgcgccggc aagcccgtcg ccggccgcgg ctacgtagag ctgactggct acgcgcgctg
     2281 agaccggtgg ctcgaaccac ccggccaggc gcggaacacg gcagccgcga ggccgtaaag
     2341 gagcatcgcc agcatcacaa ccgcgccgaa gccgaactcg atcgcaagca gcgtggccat
     2401 tgatgcgctg actaccgatg cgaagccgtt gattccccat gcccagggaa cgaaggctgg
     2461 cgcctctcct gcaacccgcg atagcccgag cgggaacggc atgcccatga aaaaggcgag
     2521 aggcgcgatc agcagcaggg agatggcgat cttctgcatg tcgggaagac cggcgaaccg
     2581 ttcgaacacg aacggcaaca gcagcaggta ggctagtgca gttgcaacga ttgcgctcac
     2641 caccagggta aggggcgagt ttcgtggcct ggcaagacgt ccgacgtaac cggaacacgc
     2701 gctgcccagt cctgcgaaca gcagga
//
LOCUS       DASUOM010000270         5869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1731947,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000270 DASUOM010000000
VERSION     DASUOM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731947"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1119)
                     /locus_tag="VFY27_05655"
     CDS             complement(226..1119)
                     /locus_tag="VFY27_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5765033.1"
                     /translation="MNGSAGKAGALRRRDVQRRFDRAAAVFDDGDFIHRHAAKGLYDR
                     MSPMLVDVRTILDAGSATGTATRELAELHRGSQIIRLDHSLNMLKHGRKRRSGFAWLS
                     RDSRVSEVQADAGLLPLRTGSIDLVFANMLMPWIDDLAAFYAEVGRVLRKDGLFAFSS
                     LGPESLLELRDAWSEVDDGEHVNAFIDMHDVGDALLHAGLREPVLDTDYLTLKYRNAA
                     GLIGDLTRAGARNCLVRRKTSLTGKNSFQKMERALDSSIKEGGLPIRLELVFGHAWGG
                     GPRPARGEFGVDIGSIGRRSR"
     gene            1209..1952
                     /locus_tag="VFY27_05660"
     CDS             1209..1952
                     /locus_tag="VFY27_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5765034.1"
                     /translation="MVPRFPDNFRRLGSALVRRAYPDCCVFCGACTFGSICFSCRADL
                     PRIENPCGRCNQPLNRIRSRERQDRKASQVDCADCQQRPPPFNHARAAVLYDFPVDVA
                     LKAFKFGHALYYVPAFAELLLAEFLLNFDASDALVPVPLHRFRYATRGFNQATELCRH
                     LGRAAGIPVVTSIRRTRWTRPQSNLDAASRRRNLEDAFAVMGRLDCRHPLVVDDVMTT
                     GETARHLARTLLDAGAKTVNVLTVARAAN"
     gene            complement(1987..2895)
                     /gene="ubiA"
                     /locus_tag="VFY27_05665"
     CDS             complement(1987..2895)
                     /gene="ubiA"
                     /locus_tag="VFY27_05665"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX5765035.1"
                     /translation="MSKNAASARPAPAFANQLRGYVQLMRLDRPVGVWLLLWPTLWAL
                     WLAGEGHPDGGVFIVFFFGVIVMRSAGCVLNDLADRKIDPHVTRTRARPLVTGVVTPV
                     EALTLFAALSLIAIGLAAMLNRPAQVLAVVAAGLTIVYPFIKRFVSIPQFVLGAAFGW
                     AVPMAFAAQTGQTEQLAWLVFGTALIWAVIYDTFYAMVDREDDLRLGVKSTAILFGDA
                     DLFVIGGLQVLMLFALVLIGQMAELGGWYMTSVGAAALLMIYHQWLARDRHPDGCFRA
                     FLHNHYIGMAIFIGIVLDYTFTLEVA"
     gene            complement(3002..5077)
                     /gene="recG"
                     /locus_tag="VFY27_05670"
     CDS             complement(3002..5077)
                     /gene="recG"
                     /locus_tag="VFY27_05670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5765036.1"
                     /translation="MHALHGELTRLTGVGPTLAEKLGRLGLHRVEDLLFFLPLRYEDR
                     TELLRPGALRPGMRCLTSGKVLLAETVFRGRRSLLVRIGDGSGQLTLRFFHFSRQQQA
                     QFRSGSVVSCYGDVRPGPVGVEMVHPEYRILKEGESPVLKEALTPVYPATEGVQQGRL
                     RSLTDQALKLMQSRPPRELLPAGVLSDLGMPSLADALTYLHRPPPDADVASIENGHHP
                     CRVRLAFEELLAHYLSLRALRNLARKEAAISLREGDAAVDEFISSLEFRLTGAQQRVL
                     AEIMADLRQPHPMMRLVQGDVGSGKTVVAAVACRKAIACGVQAAVMAPTELLAEQHLH
                     NFSAWFRDAGLEVCWLSGSLRTTGRRASLDAIASGAAQLVVGTHALFQEGVSFAKLGL
                     VVIDEQHRFGVHQRMALRDKGIGEGKFPHQLVMTATPIPRTLAMAAYADLDSSVIDEL
                     PPGRQPVLTVVMPETRRDEVVERVRSACAGGQQAYWVCPLIGESEFLDYQAAEASYER
                     LKEALPELRVGMVHGRMKPSEKDRIMRAFKLAEIQVLVATTVIEVGVHVSNASLMIIE
                     NAERMGLSQLHQLRGRVGRGAAQSSCVLLYRPPLGQLARSRLAVLRETNDGFLVAQRD
                     LELRGPGELLGTRQTGLPQYRVADLLRDAALMPRVRSTADVLQREKPEMAEAIVRRWI
                     GDSARYGKI"
     gene            complement(5134..5514)
                     /locus_tag="VFY27_05675"
     CDS             complement(5134..5514)
                     /locus_tag="VFY27_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5765037.1"
                     /translation="MKKEAVHTSGAPAAIGTYSQAIKTGRLVFLSGQIPLNPATMELT
                     GGDFEARARRVFENLKAVAQAAGGDLDRIVKVTVYLTDLANFATVNTVMAEYFAEPYP
                     ARAAVGVASLPKGADVEAEAILAL"
     gene            complement(5588..>5869)
                     /locus_tag="VFY27_05680"
     CDS             complement(5588..>5869)
                     /locus_tag="VFY27_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5765038.1"
                     /translation="EKWIAVTWEKDIDREFSSQIHVDTVNKTGALAEVAATIADSQSN
                     IEQVSVLDRHDDCSVLTFLLQVKDRNHLARIMANVRKMPNVIRVARACA"
BASE COUNT         1075 a         1937 c         1795 g         1062 t
ORIGIN      
        1 aaaccctgct ggaattacct gtcgtagccg tctctcgaca tgggcagggc acgccggttc
       61 aaaagcctgc gcagtgtact caaacgcgaa aaaccatgca aacgagaggc cagagtttga
      121 aacgcacatt cgttagccgc aacaaacagt taggctgcac tgcgacagac gatatgcagg
      181 tgtcgaagcg tacgtgaggg aggtggcaat cggcgtttcg cgccctcagc gcgagcgtcg
      241 gccgatactg cctatgtcga cgccgaactc tccccgcgcc ggccgcggac cgcctcccca
      301 ggcatggccg aacaccagtt ccaggcgaat gggcagccct ccctccttga tggaggaatc
      361 cagcgccctt tccatttttt ggaagctgtt cttgccggtg agagaggttt tccttcgaac
      421 gaggcagttg cgtgcgccgg cccgggtgag gtcgccgatg agtcctgccg cgttgcggta
      481 ctttaaagtc aggtagtccg tgtccaggac cggttcgcgg aggccggcat gcaaaagcgc
      541 atccccgacg tcgtgcatgt cgatgaacgc gttgacgtgc tcgccatcat cgacctcgct
      601 ccaggcatca cgcagctcga gcaggctttc cgggcccagg ctcgagaagg cgaacaaccc
      661 gtccttgcgc agtacgcggc caacttccgc gtagaaagcc gcgagatcgt cgatccatgg
      721 catgagcatg ttcgcgaaca ccaggtcgat gctgcccgtc cgcagcggca agagaccggc
      781 gtcagcctgc acttccgaca cacgggaatc gcgggaaagc cacgcgaacc cggagcgccg
      841 ctttcttccg tgcttcagca tattgagtga gtggtcgagg cgaatgatct ggctgccgcg
      901 atgaagctcc gcaagttccc gggtcgcggt accggtcgcc gagcccgcat cgagtatggt
      961 gcgcacgtcc accagcatcg gcgacatgcg gtcatagagg ccctttgccg catgccgatg
     1021 gatgaaatca ccatcgtcaa acaccgccgc ggcgcgatcg aagcggcgct gcacgtccct
     1081 tcgtctcaag gcgcctgcct tgccggcgct gccgttcact gcgtcgccac gcgcatctcg
     1141 caagccgttt ctgcacaaac gcccgccgtt ggccttgcac tcccgcgccg cggagcgttc
     1201 aacctgcagt ggtgccacga ttccctgaca actttcgcag gctcggcagc gcactcgtgc
     1261 gccgcgctta cccggactgc tgcgtgttct gcggtgcctg caccttcggc agtatctgct
     1321 tcagctgccg cgcagacctg ccgcgcatag agaacccctg cggccgctgc aaccagcctc
     1381 tcaacaggat ccggagtcgc gagaggcagg accggaaagc gagccaagtc gattgcgccg
     1441 actgccagca gcggccaccg cccttcaacc acgctcgcgc cgcagttctt tacgactttc
     1501 cggtggatgt tgcgctgaag gcgttcaagt tcggccatgc tctctattat gtgcccgcgt
     1561 tcgcagaact gctcctggcg gagttcctgc tgaacttcga cgcaagcgac gcgctcgtgc
     1621 cggtgccgct gcaccgcttt cgttacgcca cgcgcggctt taaccaggca acggaactgt
     1681 gccggcacct cggcagggcg gccggtattc cggtcgttac cagcatccgg cgcacacgct
     1741 ggacacggcc acaaagcaat ctcgatgcgg cgtcccggcg gcggaacctg gaagacgcct
     1801 ttgccgtcat gggccggctc gactgccgtc acccccttgt cgtggacgac gtcatgacca
     1861 ccggcgagac tgcccgccac ctcgcccgga cgttactgga tgccggcgca aaaacggtga
     1921 atgtattgac cgtggcgcgt gcggcgaact agctcgaatc aatggctgta tctgacgcca
     1981 ggcttcctac gcgacctcaa gcgtaaaggt gtagtcgagg acgatgccga tgaaaatggc
     2041 catgccgatg tagtggttgt gcaggaatgc tctgaagcac ccgtccgggt gacggtcccg
     2101 tgcgagccac tggtggtaga tcatgaggag agccgcggct ccgacggaag tcatgtacca
     2161 gccgccgagt tcggccatct ggccgatgag cacgagtgcg aagagcatca ggacctgcag
     2221 gccgccgatg acgaacaggt ccgcatcgcc gaacaggatg gcagtcgact tcacgccgag
     2281 cctcagatcg tcctcgcggt cgaccatggc gtagaacgtg tcgtagatca ccgcccagat
     2341 cagcgccgtg ccgaacacga gccaggcgag ttgttccgtc tgcccggtct gtgcggcgaa
     2401 tgccataggc actgcccagc cgaaagcggc gcccaggacg aactgcggaa tggagacgaa
     2461 gcgtttaatg aacgggtaga cgatcgtgag acccgcggcg accactgcca ggacctgtgc
     2521 gggcctgttg agcattgctg caaggccgat ggcgatcagc gacagcgcgg cgaacagggt
     2581 cagcgcctcg acgggcgtga cgactccggt taccagcggc cgggcgcggg tgcgagtgac
     2641 gtgcgggtcg atcttccggt ccgcaagatc gttcagcacg catccggccg atcgcatcac
     2701 gatcactccg aaaaagaaca cgatgaacac gccgccgtcg ggatggccct cgccggccag
     2761 ccagagcgcc caaagcgtag gccagagcag cagccagacg ccgacgggcc tgtcgaggcg
     2821 catcaattgc acgtagcctc taagctggtt cgcgaaagcc ggcgccggac gcgcactcgc
     2881 agcgtttttg ctcatagatc tgataccagc tcgttctctc cgggaaagca tcccgcactt
     2941 gcctcgaaga gtcgcatcgg tcgggggcta aagtaccaga tcgaattcaa ggtatgcagc
     3001 atcagatctt gccgtagcgc gccgaatcgc cgatccagcg gcgtacgatg gcttcggcca
     3061 tctccggctt ttcccgctgc aatacatcgg ccgtgctgcg cacgcgcggc atcagcgccg
     3121 cgtcgcgaag caggtccgcg acgcggtatt gaggcaggcc ggtctgccgc gttcccagca
     3181 attctcccgg gccgcgcaac tccaggtcgc gctgggcaac gaggaacccg tcattcgttt
     3241 cacgcaatac cgcgagccgg ctgcgtgcaa gctggccgag cggcggccgg tacaaaagga
     3301 cgcagctgct ttgcgcggcg ccgcggccga cccgcccgcg caactggtgc agctgggaga
     3361 gccccattct ttccgcgttc tcgatgatca tgaggctggc attggacaca tgcacgccga
     3421 cctcgatgac ggtcgtggcg acgagcacct ggatctcggc cagcttgaac gcgcgcatga
     3481 tgcggtcctt ttcggacggc ttcatccgcc cgtgcaccat cccgacccgc aactccggca
     3541 gcgcttcctt aagcctctcg taactcgctt ccgcggcttg gtaatcgagg aactccgact
     3601 cgccgatcag ggggcagacc cagtacgcct gctggccgcc ggcgcaggca gaccttacgc
     3661 gttccacgac ctcgtcacgc cgcgtctccg gcatcacgac tgtcagcacg ggctggcgcc
     3721 cggggggcag ttcgtcgatc accgaagagt cgaggtccgc gtacgccgcc atcgccaggg
     3781 tgcgcggtat cggcgtcgcc gtcatgacca gctgatgggg aaatttgccc tcgccgatac
     3841 ctttgtcgcg cagcgccatc cgctggtgca ccccgaaccg atgctgctcg tcgatcacca
     3901 cgagtcccag cttggcgaag gacacacctt cctggaacag tgcgtgggtg cccacgacca
     3961 gttgcgcagc gccgctcgcg atcgcgtcga gagacgcccg ccgcccggtg gtccgtaaac
     4021 tgccgcttag ccagcagacc tccaggccgg cgtcgcgaaa ccacgcggag aaattgtgga
     4081 gatgctgctc ggccagtagc tccgtcggcg ccatgaccgc cgcctgcact ccgcaggcga
     4141 tggctttgcg gcacgccacc gccgcaacga ctgtcttgcc cgatcctacg tccccctgca
     4201 ccagccgcat catcgggtgc ggctggcgca agtccgccat gatctcggca agtacccgtt
     4261 gctgcgcgcc ggtcaggcga aactcgaggc tcgaaatgaa ttcgtcaacg gcagcgtccc
     4321 cttcccgaag ggaaatagcc gcctccttcc gggcaaggtt tctaagggcg cgcaggctta
     4381 agtaatgcgc cagcaattct tcgaatgcga gcctgacccg gcaggggtga tgaccattct
     4441 cgatacttgc gacatcggca tcgggcggcg gccggtgcag gtaggtcagg gcatcggcga
     4501 gcgaaggcat tccgaggtcg cttaaaactc ctgccggcag gagttcccgt ggcggcctcg
     4561 actgcatcaa tttgagtgcc tggtccgtca ggctgcgcag ccttccctgt tgcactccct
     4621 ctgtggccgg ataaacgggg gtcagcgcct ccttaaggac gggactctca ccttccttga
     4681 ggatgcggta ctccgggtgc accatctcga cgccgactgg cccgggccgc acgtcgccat
     4741 aacagctgac gacggagccg gaacgaaact gcgcctgttg ttgcctcgag aaatgaaaaa
     4801 accgcagcgt gagctggccg gagccatccc cgatacgcac cagcaggctg cggcggccgc
     4861 ggaataccgt ctcggcaagc agtaccttgc cactggtcag gcagcgcatc cctggccgga
     4921 gcgctcccgg ccggaggagt tccgtgcggt cctcgtagcg cagcggcagg aaaaacaaca
     4981 ggtcctcgac gcggtgcagt cccagtcgcc cgagcttttc cgcaagcgtc gggccgacgc
     5041 cggtcagtct cgtcagttcc ccatgcagcg catgcactcc tgacccctgg actgccgtct
     5101 cgattggctc ttggatcgcg atgaaccttt agattacagg gccaggatcg cctcggcctc
     5161 cacgtccgcg ccttttggca gcgacgcgac tccgacggcg gcacgcgccg ggtaaggctc
     5221 ggcgaagtat tccgccatca cggtattcac cgtagcgaaa tttgcgagat cggtaaggta
     5281 gacggtgacc ttcacgatcc tgtcgaggtc gccgcccgcc gcctgtgcaa ccgccttcag
     5341 gttctcgaag acgcgcctcg cgcgggcctc gaagtcgccc ccggtcagtt ccatcgttgc
     5401 cgggtttagc ggtatctgcc ccgagagaaa aacgagcctg ccggtcttta tcgcctgcga
     5461 gtaggttccg atcgctgcag gcgcaccgct ggtgtggacg gcttcttttt tcaatgtcct
     5521 ggtctcccct gtctaaaagt gacttggtgt tttcattcag cgcgagacgc tacagcggcg
     5581 accgggttca ggcacaggcg cgcgcgacgc gtatgacgtt cggcatcttg cgaacgttgg
     5641 ccatgatccg ggcgaggtgg ttcctgtcct tgacctgcag caggaacgtc agtaccgagc
     5701 aatcgtcgtg ccggtccagc accgatacct gctcgatgtt cgactgcgag tctgcaatcg
     5761 tcgctgccac ctctgccagg gcccctgtct tgtttaccgt atcgacgtgg atctgactcg
     5821 aaaattcgcg gtcgatgtcc ttctcccagg tcaccgcgat ccacttctc
//
LOCUS       DASUOM010000271         6411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1256777,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000271 DASUOM010000000
VERSION     DASUOM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256777"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="dapA"
                     /locus_tag="VFY27_05685"
     CDS             complement(<1..766)
                     /gene="dapA"
                     /locus_tag="VFY27_05685"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5765039.1"
                     /translation="MGGDLVFTGSLVALVTPFDADDRIDYAAVERLVEFHVAEGSDGL
                     VIAGTTGESATLERDEHAELIGRAVEIVAGRLPVIAGTGSNSTRQTMDLSLAVGKSKI
                     DAYLLVTPYYNKPVQEGLFRHFSAIADVVEKPVILYNVPGRTAADTLPETVGRLAKHP
                     RIIGIKEATGDMQRLKDIKALVPENFRLYSGDDFTAREFIDLGGHGTVTVTGNVAPRL
                     MSDMCRAQAANDRGAAVVLDAKLQPLNKAMFLESNPI"
     gene            920..1450
                     /locus_tag="VFY27_05690"
     CDS             920..1450
                     /locus_tag="VFY27_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HEX5765040.1"
                     /translation="MKQLIVLSAVGNDRHGVVNDISKVILDCGGNIEESRMSALGNEF
                     AMLLLISGNWHTLAKLESELDKLTKAHSLTVTIRKTGERRDKTDCIPYGVDVVALDQP
                     GIVFNLASFFSSRGIDIAELATRRYAAAHTGASMFSAQMTVNIPSSVHLAQLRDEFLE
                     LCDQLNLDSILEPVKS"
     gene            1515..1982
                     /locus_tag="VFY27_05695"
     CDS             1515..1982
                     /locus_tag="VFY27_05695"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004164030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5765041.1"
                     /translation="MSAPVLNKVVPDFTCAATGGKPIQLKALRGKKVVLYFYPKDSTP
                     GCTTEGQDFRDLHAKFKRAGAIVLGVSRDSLASHEKFKEKLKLPFELLSDPDEALCKQ
                     FDVIREKSLYGRKFLGVERSTFLIDDTGRLRREWRKVKVNGHAAGVLEAAKEL"
     gene            2150..3625
                     /locus_tag="VFY27_05700"
     CDS             2150..3625
                     /locus_tag="VFY27_05700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5765042.1"
                     /translation="MKTPFSNFVLCLIACTGLVFAPVAPGAEVNLPDIGSPADAILSK
                     SEEARIGQAIMRSIRASGKLVEDPQITEYVNEIGHRVASYANDGEYAFTFFVVDDPNI
                     NAFALPGGYIGVHTALLEATRNEHELAGVLAHEIAHVTQRHIARAVHASQRQSLLSTA
                     IMLGAILAGAAGGSGDAMQGAIAVAQGTAAQQQINFTRSNEYEADRVGIAALADAGFD
                     PQGMASFFEVISRETRPVESRMPEFLRTHPVSSARIAEARGRARNYDANAVPNTTDYG
                     IAQARLQVSRHETAEAAVSFFEKKSYESQTDTERYGRAVAYSRAGRHNEANRIYEELA
                     SKDQKVIAYHIGLAQSQLALEQIDEARATFERARELFPRNVPLTVHYAEALLQLGEPK
                     LAHKILLDLFNNVQPTPEQVRLIARAAIDAGDTAEAHYYMSEYSLMIGDLVQGVHFLR
                     QALAVPELQEIQRIRFEARIDFIREYMTEEQLQQMQRSRQS"
     gene            3706..4521
                     /locus_tag="VFY27_05705"
     CDS             3706..4521
                     /locus_tag="VFY27_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5765043.1"
                     /translation="MLENSALRLPVLSLMLAILALAGCATNSAATREGGEPTGAESAA
                     DPWEQMNRRIFRFNRDVDRATLKPVAIGYEKVIPSFVRSGIRNFYTNLRGPRNILNNF
                     LQGKGASGFSETGRFVVNSTIGILGLVDVASGLGLEKREEDFGQTLAVWGVPEGPYVM
                     VPFAGPQTLRDAFAFPMDVLVDPLWYWLYDENAPVRYGLYALRAINFRAGFLSAEALL
                     DQSFDPYVRLREAYLQNRLYEVYDGNPPVDDDFYDEMYDDLPEPDPAPQDDQQ"
     gene            complement(4541..4879)
                     /locus_tag="VFY27_05710"
     CDS             complement(4541..4879)
                     /locus_tag="VFY27_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5765044.1"
                     /translation="MSRYELMDKGDGNFSLTGEMTFDTAGDILRDSEAPFEKHTRIEV
                     DLSRVTDTDSAGLALLLEWITWANHTVREIRFRGVPPIIDAIAKTTEVDNLLTKGERW
                     SGFIEAPPAA"
     gene            complement(4876..5526)
                     /locus_tag="VFY27_05715"
     CDS             complement(4876..5526)
                     /locus_tag="VFY27_05715"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5765045.1"
                     /translation="MRKLLLAVTLGLASLSISQIASAQQAPDEFVREAAELLDEAVSE
                     RKQELASDRQALYELINEILLPRFDRRYAAQLVLGRHWRTASEEQREQFIEAFYQSLM
                     RQYADGLLEFDLSQLEILPYRGDPTEPRTVVRTVVKLDDGTEAPVDYGLVNKDSGWQV
                     FDVTIEGISYVRNFRAEFNSEIQATDLDAVIQRLQREAAQGPSEEAAGPEEATATE"
     gene            complement(5558..6043)
                     /gene="mlaD"
                     /locus_tag="VFY27_05720"
     CDS             complement(5558..6043)
                     /gene="mlaD"
                     /locus_tag="VFY27_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX5765046.1"
                     /translation="MQQTRSVELGTGLFVVLGFAALFFLATQTTGSETFSGTETYEVQ
                     ARFQNVGNLKTRAPVSMSGVTIGRVRSVELDPEQLNAVVTFAIDNRYDEIPDDSDASI
                     LTAGLLGSQFIGLQAGGSDTYLADGSEIQFTQSAVVIENLISKYLFNSGGRDSSNDKS
                     E"
     gene            complement(6053..>6411)
                     /locus_tag="VFY27_05725"
     CDS             complement(6053..>6411)
                     /locus_tag="VFY27_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765047.1"
                     /translation="VRRVLVPRFLGGVISMPLLTAVFNVIGLFGAHVVAVGLLQVDAG
                     AFWQQLQANVGAGDVYEGIIKSVVFGIVASLLAVWEGYNSVPTAEGVGRATTRAVVRT
                     AIAVLILDFMITAVAI"
BASE COUNT         1342 a         1936 c         1834 g         1299 t
ORIGIN      
        1 gtatagggtt cgactccagg aacatcgcct tgttcaaggg ctgcaatttg gcatccagta
       61 ccactgcggc gccgcgatcg ttcgcagcct gagccctgca catgtcggac atcagccgtg
      121 gcgcgacatt gcccgtcacc gtcacggtgc catgaccccc gagatcgatg aactcgcggg
      181 cagtgaagtc gtcgccgctg tacagcctga agttctctgg aaccagagcc ttgatgtcct
      241 tcaaacgttg catatccccg gttgcttcct tgatgccgat gatcctcggg tgcttcgcca
      301 ggcgcccgac cgtttccggc agggtgtcgg cggcggtgcg tcccggcacg ttataaagaa
      361 tgaccggctt ctccactacg tccgcgattg cagagaaatg ccggaacagg ccttcctgta
      421 ccggtttgtt gtaatacggc gttaccagca aataggcatc gatcttagac ttgcctacag
      481 ccagcgacag gtccatcgtc tggcgagtcg agttggagcc ggtcccggca atgaccggca
      541 gccggccggc cacgatttcc acggcgcgtc cgatcagttc ggcgtgctcg tccctttcga
      601 gggtcgctga ctcgccggtg gtgccggcaa tgaccaatcc gtcactgccc tccgccacgt
      661 gaaactcgac caggcgctcc acggcggcgt agtcgatgcg atcgtcagca tcgaacggtg
      721 tcaccagcgc caccaggctg cccgtgaaca caagatctcc tcccacaaat aaggcggcga
      781 tgttactgtt cgcttcccgg agtggcaagg cagatgcagg gacgtcgcta tttgacttat
      841 gcttcccgct atttgcgagg aatgcggcgg taacgcgcgc cgcatttctt cgtgctgcct
      901 tcatcgataa tcgagataaa tgaagcaact cattgtttta tctgcggtag gcaacgaccg
      961 gcacggcgtc gtcaacgaca tcagcaaggt cattctcgac tgtggcggca acatcgaaga
     1021 aagccgcatg agcgccctcg gaaacgagtt cgccatgctg ttgctgattt ccggcaactg
     1081 gcacacgctg gcgaagctcg agtccgaact cgacaagctg acaaaggctc attccctgac
     1141 ggtgacgatt cggaagaccg gcgaacgccg ggacaagacc gattgtattc cttacggggt
     1201 cgatgtcgtg gccctggacc agccgggcat cgttttcaac cttgcgagtt tcttttcaag
     1261 ccgtggtatc gacatcgctg aactcgccac ccggcgttac gccgcggcac ataccggcgc
     1321 ttccatgttt tcggcgcaga tgacggtgaa catcccgtcg tccgtacacc tcgcgcaact
     1381 gcgcgacgag ttcctcgaac tgtgtgacca gcttaacctc gactccatac tcgagcccgt
     1441 aaaatcctga ctggcaagat tgaaatcggg attggccagc catagatatc ccgcttctgc
     1501 acgcgaggaa tcggatgagc gctcccgtcc tgaacaaagt tgtccctgac ttcacctgcg
     1561 ccgcgacggg tggaaagccg atccagctca aggccctccg cggcaagaag gtggtgctct
     1621 acttctatcc gaaggacagt acgcccggtt gcacgacgga ggggcaagac ttccgggatc
     1681 tgcatgcaaa gttcaagcgc gcgggtgcta tcgtgcttgg cgtctcgcgc gacagcctcg
     1741 catcgcatga aaagttcaag gaaaaactga agctgccttt cgagctgctc tcggatccgg
     1801 acgaagcgct gtgcaagcag tttgacgtca ttcgggagaa atccctttat ggccgcaagt
     1861 tcctgggggt agagcgcagc accttcctga tcgatgacac cggaagactt cggcgggagt
     1921 ggcggaaggt caaggtaaac gggcacgccg ccggggtgct ggaagctgcc aaggagcttt
     1981 gaggcaccgt cctcccgaag gttctattga aatcatcata tttaacaaat tcttaacctg
     2041 tatacctaga cgccaagtta gcacgtggag gagctgcgct ggtagtatca tgggaacgct
     2101 cagggtcggg gacagtcgga gcagtagtcg tttcgcaatg agtacttggg tgaaaacacc
     2161 gttctcaaac ttcgtgttgt gcctcattgc ctgcacgggt cttgtgttcg cgccggtcgc
     2221 gccgggagca gaggtcaacc tgcccgacat cgggagtccg gccgatgcca tcctgtcgaa
     2281 atcggaagag gcgcgcatcg gccaggcgat catgcgcagt attcgggcga gcggcaagct
     2341 ggttgaagat ccgcaaatca ccgagtacgt gaacgagatc ggtcaccgcg ttgcctccta
     2401 tgccaacgat ggcgagtacg ctttcacgtt cttcgtcgtc gacgacccca acatcaacgc
     2461 ctttgccctg cccggcggct acatcggcgt gcataccgct cttctcgaag ccacccggaa
     2521 cgaacacgag ctggccggcg tactggctca tgaaattgcc cacgtgacac agcggcacat
     2581 tgcgcgtgct gtgcacgcga gccagcgtca gagcctcctc agcacggcta tcatgctcgg
     2641 ggccattctc gccggcgctg ccgggggcag tggagatgcc atgcagggtg cgatcgccgt
     2701 cgcccagggc acggctgcgc agcagcagat caacttcacg cgcagcaacg agtacgaagc
     2761 ggaccgtgtg ggtatcgccg ctctcgcgga tgcgggtttc gatccgcagg ggatggcctc
     2821 ctttttcgag gtgatatcga gggagacccg gcctgttgag tcccgcatgc cggagttcct
     2881 gaggacccac cctgtcagca gcgcccgtat tgcggaggca cgcgggcgcg cccgaaacta
     2941 cgacgcgaat gctgtaccga atacgacaga ctacgggatc gcgcaggcac gcctgcaggt
     3001 gtccaggcac gaaacagctg aagcggcagt cagctttttt gaaaaaaaga gctatgagag
     3061 ccagacggat acggaacgct acggtcgcgc cgtagcatat tcccgcgccg gccgccacaa
     3121 tgaagcgaac cggatttacg aggaactggc gagcaaggac caaaaggtca tcgcttacca
     3181 catcgggctg gcccagtcac agctggcctt ggaacagatc gacgaagctc gtgcgacttt
     3241 cgagcgcgca cgcgagctgt tcccccgaaa tgtgccgctc acggtgcatt acgccgaagc
     3301 cctgctgcag ctcggcgaac cgaagctcgc gcacaagatc ctgctagacc tgttcaacaa
     3361 cgtgcaacca acaccggaac aggtgcggct gatcgcccgg gctgcgatcg atgcgggcga
     3421 cacggcagaa gcccactatt acatgtcaga atacagcctc atgatcggcg accttgtgca
     3481 gggagtgcat ttcctcaggc aggcgcttgc agtgccggag ctccaggaaa ttcagcggat
     3541 ccgcttcgag gctcgcatcg atttcatccg cgagtacatg acagaggagc aattgcagca
     3601 aatgcagaga agtcggcaat cttgattctc cccgggcata tgtcggaact gccttcgcgc
     3661 ctctgtctct gaccttcccg actcactaac caagaataat cgtccatgtt ggaaaactcc
     3721 gcgttgcgcc tcccggtcct gagtctgatg ctggcgatac tggcactcgc aggttgtgcc
     3781 acgaattcag cggccacacg cgagggaggc gaaccgaccg gcgccgagag cgccgcagat
     3841 ccatgggaac agatgaatcg gcgcatcttc cggttcaacc gggatgtcga ccgtgcgacg
     3901 ttgaagcctg ttgctatcgg ctatgaaaag gtgattccgt cgttcgtccg aagcggcatc
     3961 aggaacttct atacgaacct tcggggaccc aggaatatcc tcaacaactt cctgcaggga
     4021 aaaggcgcga gcggctttag tgaaaccggc cgatttgtcg tcaacagtac aatcggaatt
     4081 ctgggtcttg tggacgtcgc atccggactt ggactcgaga aacgcgagga ggatttcggt
     4141 cagactcttg ccgtctgggg tgtcccggaa ggcccctatg tcatggtccc ctttgcaggc
     4201 ccgcagacgt tgcgcgatgc atttgcgttt ccgatggacg tacttgtaga cccgctatgg
     4261 tactggctct acgacgaaaa cgcgccggtc cgctatggcc tctacgcgtt gcgcgccatc
     4321 aactttcgcg ccgggtttct gtccgctgaa gccctgctgg atcagtcgtt cgatccctat
     4381 gtcaggttgc gcgaggccta tctgcagaac cgcctctacg aggtctacga cggcaatccg
     4441 ccggttgacg atgatttcta cgatgaaatg tacgacgatc tgcccgagcc cgatccggct
     4501 ccgcaggacg atcagcaata agtcccagcg tcggaaagcc tcaggccgcc ggcggcgcct
     4561 cgataaatcc cgaccaacgc tcgcctttgg tcaggaggtt atcgacctcg gtggttttgg
     4621 caattgcatc gatgatcggc ggcactcccc tgaagcgtat ctcccgaacg gtatggttgg
     4681 cccaggtgat ccactccagc aatagcgcga ggccggccga gtccgtgtcc gtgacgcggg
     4741 aaaggtccac ctcgatgcgc gtgtgctttt cgaatggcgc ttcgctgtcg cgaagtatgt
     4801 cgcccgcagt atcgaacgtc atctcgcccg tgagcgagaa gttgccgtcc cctttgtcca
     4861 tgagttcgta gcgactcatt ccgtagcagt tgcttcttcg ggacccgcag cttcttcgct
     4921 cggaccctgt gccgcttccc gctggagccg ctggatgacg gcatcgaggt cggtcgcctg
     4981 gatctccgaa ttgaattccg cacggaaatt tctcacgtag gaaatgcctt cgatcgtgac
     5041 gtcgaagacc tgccagccgg aatctttgtt aaccagaccg tagtccaccg gcgcttccgt
     5101 gccgtcgtcg agcttcacga cagtccgcac tacggtacga ggttcagtcg ggtcgccacg
     5161 gtacggcagg atttccagtt ggctcaggtc gaactcgagc aggccgtcgg cgtactgccg
     5221 catcaaagac tggtagaacg cctcgataaa ttgctcgcgc tgctcctcgc tggccgttcg
     5281 ccaatgcctg ccgaggacta gttgcgccgc atagcgccga tcgaaccgtg gcagaaggat
     5341 ctcgttgata agttcgtaga gcgcctgcct gtcggaggca agttcctgct tgcgttcgct
     5401 cactgcctca tcgagaagct ccgctgcttc cctgacaaac tcatccggag cctgctgtgc
     5461 cgacgcgatc tgcgaaatac tcagcgacgc aagcccgagc gttacggcaa gtagaagttt
     5521 tctcactatg ctgcctccat ttgacagaat ccggacatta ttcactcttg tcgttgctgc
     5581 tgtcacgacc gccactgttg aacaggtact tgctgatgag attctcgatg accacagccg
     5641 actgggtaaa ctgaatttca ctgccatcgg caagatacgt gtcggaaccg ccggcctgca
     5701 aaccgatgaa ctggctgccg agaaggccgg cggtcaggat gcttgcatcc gaatcgtcgg
     5761 gaatttcatc gtaccggttg tcaatggcaa aggtgacaac ggcgttcagc tgctccgggt
     5821 caagttccac cgacctcacc ctgccgatag tcacgcctga cattgagacc ggggcacggg
     5881 tcttgaggtt cccgacgttc tggaaccttg cctgtacttc gtaggtttcc gtgccagaga
     5941 aggtctcgga acccgtggtc tgggtcgcca gaaagaacag agcagcgaaa cccaggacca
     6001 caaaaagtcc ggtacccaat tccacagatc gtgtttgttg catgtcgcta cctcaaatgg
     6061 caacagcggt aatcataaaa tcgagaatca gcacggcgat cgccgttcgc acaaccgcgc
     6121 gcgtcgttgc ccggccgacg ccctcagcgg ttggaaccga attataacct tcccacaccg
     6181 ccagcaggct cgcaacaatg ccgaacacta cgctcttgat gattccttca tagacgtcgc
     6241 ctgcccccac attcgcctgc aactgctgcc agaaggctcc ggcatcgacc tgcagcaatc
     6301 cgacggcaac cacatgcgcg ccgaaaagcc cgatgacatt gaacaccgca gtcagtaaag
     6361 gcatcgagat aacgccgccg agaaatctcg gcacgagcac ccgacgtacc g
//
LOCUS       DASUOM010000272         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_712821,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000272 DASUOM010000000
VERSION     DASUOM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712821"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFY27_05730"
     CDS             complement(<1..259)
                     /locus_tag="VFY27_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5765048.1"
                     /translation="MDVENFPDIDHLLRLLAEQSVEHAILLLDTEGRIRWWSPGAEHI
                     FDIPGSEAVGQHSSRIFTPEQVAEGVPEHEMSVARTNIAAED"
     gene            complement(274..2439)
                     /locus_tag="VFY27_05735"
     CDS             complement(274..2439)
                     /locus_tag="VFY27_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEX5765049.1"
                     /translation="MNARPTPLYDHNRYWAECFGTAPFLPTSRREMDALGWDSCDIVI
                     VTGDAYVDHPSFGMAIIGRLLEAQGFRVGIVAQPDWRSKDAFEALGRPNLYFGVAAGN
                     MDSMINRYTADRKIRNDDAYTPGGVAGKRPDRCSLVYSQRCKEAYKDVPVVLGGIEAS
                     LRRIAHYDYWQDKVRRSILFDASADILVYGNAERAIVDLSHRLARGEAIESITDLRGT
                     AFIRRDRPEGWWEIDSTRIDRPGRVDQVVNPYLNTQELRECASAKAEQGNGGDPPARV
                     LRFVPEARRNRSRTVIRLPSFEKVRNDAILYAHANRVLHLETNPGNARALVQAHGHQD
                     LWINPPALPLTTEEMDYVFGTAFARRPHPSYGNERIPAYEMIRFSVNIMRGCFGGCTF
                     CSITEHEGRIIQNRSEESILGEIEDIRDKVPGFTGVISDMGGPTANMYRLACKSKEVE
                     SACRRPSCVYPGICSNLNTDHSALTGLYRKARALPGVKKVLIASGLRYDLAVKDPEYV
                     RELVTHHVGGYLKIAPEHTEEGPLHKMMKPGIGAYEEFRELFEKFSGEAGKEQYLIPY
                     FIAAHPGTTDQDMVNLALWLKKNDFRADQVQAFYPAPMATATAMYHSGKNPLRKVTYK
                     SDAVHVVKEPEKRRLHKALLRYHDPDNWPLIRKTLEEMHLGHLIGAGKEQLVPSDQPK
                     TVPAHRAPRRKNTKEAHAKRLKRNRVLTQHTGLPPRSSR"
     gene            2690..>3894
                     /locus_tag="VFY27_05740"
     CDS             2690..>3894
                     /locus_tag="VFY27_05740"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5765050.1"
                     /translation="MRNFLTVLSIAALLAACGDAKDDQSITTPAAEEPKTTSAPGATE
                     AETARLNEWFEARWEEQLDFSPIRKTFLGRKDDYDKIDDLSEAAEDEQLAWRRRTTAE
                     MKESFDYDSLTDEAKMSYDVWVYELERAERALPFRNLDYVFTQMQGPQAFLPQFLIAF
                     HKVSGQSDMEAYITRISGISRGLDQLLERAKLGAEGGVRPPKFAYEGVLQQARALVTG
                     EPFGGEGEAPLWSDANTKIEALQKDGKIDEAKANELRDRAREALLKSFKPAYDRLIAW
                     VESDLSNSSAEAEGVGALPNGIAYYDERLAFATTTDMTADEVHDYGLAEVARIRAEME
                     QIKSDVGFEGTLQEFFVFLREDPQFFFPNTDEGREGYIQAAREHLDYIEQSLPEYFGI
                     LPKADLEVKR"
BASE COUNT          744 a         1191 c         1205 g          754 t
ORIGIN      
        1 cgtcctccgc ggcgatgttc gttcgagcca cggacatctc gtgctcggga actccttcag
       61 caacctgctc cggcgtgaat atgcgcgaac tgtgctggcc caccgcttcg ctgccgggaa
      121 tgtcgaatat gtgctccgca cccgggctcc accacctgat acgcccctcc gtgtccaaca
      181 gcaatatcgc atgttcgact gactgctccg ccaacagccg cagaagatgg tcgatgtccg
      241 gaaaattctc gacgtccatt ccggaacctg ccgtcacctg ctgctgcgcg gtgggaggcc
      301 cgtatgctgc gtcagcaccc ggtttcgctt cagccgcttt gcgtgcgctt ccttcgtgtt
      361 cttgcgacga ggagcccggt gcgccggcac ggtcttcggc tgatccgatg gcacgagctg
      421 ctccttgccc gcaccgataa ggtgaccgag atgcatttcc tccagggttt tgcggatcag
      481 cggccagttg tccggatcgt gatagcgcag aagcgccttg tgcagccgcc gcttttccgg
      541 ttctttgacg acgtgcacgg cgtcgctctt gtacgtcacc tttcgcagcg gattctttcc
      601 ggagtgatac atcgcggtgg ccgtcgccat gggcgcagga tagaacgcct gcacctggtc
      661 agcgcggaaa tcgttcttct tgagccacag cgccaggttc accatgtcct ggtccgtggt
      721 gccagggtgg gcggcgatga agtagggaat caggtactgc tccttgccgg cttcccctga
      781 aaacttttcg aacagttcac gaaattcttc ataggcgccg atgccgggct tcatcatttt
      841 atggagcggc ccttcttccg tgtgctccgg cgcaatcttc aggtagccgc caacatggtg
      901 ggtgaccagt tccctgacgt actccgggtc tttcaccgcg aggtcgtagc gaagtcccga
      961 ggcgataagc accttcttga cccccggcaa tgcacgcgcc ttccggtaca ggccggtcag
     1021 ggccgaatgg tccgtgttca ggttgctgca aatgccggga tagacacagc tcggccgccg
     1081 gcaagccgac tcgacctcct tgctcttgca agccaggcgg tacatgttcg cggtaggccc
     1141 gcccatgtcc gagatgacgc cggtgaagcc gggcaccttg tctcgaatgt cctcgatttc
     1201 accgagtatc gattcctcgg agcgattctg gatgatgcgg ccctcgtgct cggtgatcga
     1261 gcagaacgta cagccgccga aacagccgcg catgatattc accgagaagc ggatcatctc
     1321 gtaggcggga atgcgctcgt tgccgtacga cgggtgcggc cggcgggcga acgccgtacc
     1381 gaagacgtag tccatttctt ccgtggtgag tggaagcgcc ggcggattga tccagaggtc
     1441 ctggtggcca tgcgcctgca ccagcgccct cgcgttgccg ggattcgttt ccaggtgcag
     1501 tacacgattc gcgtgcgcgt aaagtatcgc atcgttcctc accttctcga acgacggcag
     1561 gcgtatgact gtgcgcgaac ggttgcggcg cgcctccggc acgaaccgca gaaccctggc
     1621 gggagggtca ccgccgttgc cctgttcagc cttggcggat gcacattctc gtagctcttg
     1681 cgtgttcaaa tacggattca cgacctggtc gacccggccc gggcgatcga tgcgggtcga
     1741 gtcgatttcc caccacccct ccggcctgtc gcgacgaatg aatgcggtgc cgcgaagatc
     1801 ggtgatgctc tcgattgcct cgcctcgcgc cagcctgtgc gacaaatcca cgattgcacg
     1861 ttccgcgttc ccgtacacca ggatatcggc gctggcatcg aacaggatgg agcggcggac
     1921 cttgtcctgc cagtaatcgt agtgcgcgat gcggcgaagc gaggcctcga tgccgccgag
     1981 caccaccggc acgtccttgt aggcctcctt gcagcgttgc gaatagacga gggagcagcg
     2041 gtcaggacgc ttgccggcaa cgccgcccgg cgtgtaggca tcatcgttcc ggatcttccg
     2101 gtctgcggta taccggttga tcatcgagtc catgttgccg gcggcaacgc cgaagtagag
     2161 gttcggccgc cccagcgctt caaacgcatc tttggaacgc cagtcgggct gcgcgacgat
     2221 acccacgcga aacccctgcg cctcgagcaa gcgcccaatg atcgccatgc cgaacgacgg
     2281 gtgatccacg taggcgtcgc cggtaacgat gacgatatcg caggaatccc agccgagcgc
     2341 atccatttcc cgcctcgagg tcggcaggaa cggcgcggtg ccgaagcatt ccgcccagta
     2401 gcggttatgg tcgtacaggg gcgtcggacg ggcattcatg ggtttcggta ccggcgggtg
     2461 ggtggaaagc ggtcggacca gtcgttcagc ttaggcgtta ccgcccgcag ggatcaacca
     2521 tttgcgaatg aaggcgctcc tcgggacgaa gagcccgcag gcttcctgct tgtgatcctt
     2581 ctccgaaatt gcgaaggtta gcgactttcc cgaaccggat aaaccgtcac aagtacctgt
     2641 aagacgctgg cagacgacgt tccggtcgct cgcccaggag gatcccgaga tgcgaaattt
     2701 ccttaccgtg ctttcgatag cggcactgct cgccgcctgc ggcgatgcga aagacgacca
     2761 atcgataacg acacccgcgg ccgaggaacc caagacaacg agcgctcccg gtgcgaccga
     2821 agccgagacg gcgcggctca acgagtggtt cgaggcccgc tgggaggaac agctcgattt
     2881 cagcccgatc cggaagacct ttctaggacg caaggacgac tacgacaaga ttgacgatct
     2941 ttccgaagct gccgaggacg agcagctcgc gtggcgccgc cgcaccactg cggaaatgaa
     3001 ggaaagcttc gactatgaca gcctgacgga cgaggcaaag atgtcttacg acgtctgggt
     3061 ctatgaactc gagcgcgcag agcgcgcgtt gccgttccgc aatcttgatt acgtattcac
     3121 acagatgcag gggccgcagg cattcctccc gcagtttttg attgccttcc acaaggtctc
     3181 cgggcaaagc gacatggagg cctatatcac acgcatatcc ggaatctccc gtggcctcga
     3241 ccagcttctc gagcgggcaa agctgggagc cgaaggaggc gttcgtccgc cgaaattcgc
     3301 atacgaaggc gtacttcaac aggcgcgagc actggtcacg ggcgagccgt tcggcggcga
     3361 gggcgaggct ccgctgtggt cggatgcgaa cacgaagatc gaggcgctgc agaaggatgg
     3421 caagatcgac gaggcgaagg cgaacgagct gcgggatcgc gcgcgtgagg cgctcctcaa
     3481 gagcttcaag cccgcgtatg acaggctcat cgcctgggtc gagtcggatt tgtcgaacag
     3541 cagcgccgag gcagagggtg tcggggcgct cccgaacggg atcgcgtatt acgatgaacg
     3601 cctcgccttc gcaaccacaa ccgatatgac ggcggacgag gtgcacgact acggcctggc
     3661 cgaggtcgca cggattcgtg ccgagatgga gcagatcaag tcggacgtcg gtttcgaggg
     3721 aacgctgcag gagtttttcg tgttcctgcg ggaggaccca cagttcttct tcccgaatac
     3781 ggatgaaggg cgtgaaggct acatccaggc agcacgcgag catctcgatt acatcgagca
     3841 gagcctcccc gagtactttg gcatccttcc gaaggccgat ctcgaggtga aacg
//
LOCUS       DASUOM010000273         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_505725,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000273 DASUOM010000000
VERSION     DASUOM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505725"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VFY27_05745"
     CDS             <1..1551
                     /locus_tag="VFY27_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765051.1"
                     /translation="LLLIVSGLVGRRRLLSALTPIADLQKPIAELAKGNLSVQFKPAE
                     HREISDIVEALESTASALSERNEKLSRLANHDVLTGLFNRRRFVEELRKEVRNVARER
                     NTAALLFVDLDQFKYVNDTCGHPAGDRLIRKVADQLQRCVGASGTVGRFGGDEFTVLA
                     TRVSKPEAQALAESILEDLRKVAHVEDDNVFHVHCSIGVAMIDSDRFDHDDLISQADI
                     ACREAKASGRNRLAFHSMSDNDVEQVAADVSWVSKLREAIDNNLLMLRYQPIVCIATG
                     ETTHHEVLLRMRSDNGDMVSPNAFLPAAVRFGLMAEIDTWLIETAIAELARYRQDAPD
                     LRFALNLSANAFEAENLTAFVMSQLARQNVPPECVTFEITESLAMRHLNHVEKQIAGL
                     REIGCQVSLDDFGTGYSSFSYLQKLPVDYIKIDGSFIRDLVRNPVDQKMVRMIGEIGK
                     AAGMKTIAEYVQSGAAFTLLGRLGIDFAQGFYIGRPAATPIRKSMPVPIQSHKEQKRK
                     HQRKRNTS"
     gene            1921..>3054
                     /locus_tag="VFY27_05750"
     CDS             1921..>3054
                     /locus_tag="VFY27_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5765052.1"
                     /translation="MPHALIVDNDVENSQTLSQVFRECGYLPETVESLPQAREALLRH
                     TPEIAILNEQLAGDDTLGLLESINLAPVTEIYLMSDQPNPRITPRAIRIGASDFFRKP
                     VNKDRLARNLRDLSTELSSTTPAAGAPSPRGDALVGESKAMRRLYRLLRKCAPGDASI
                     FLAGESGSGKELVARTIHQLSDRAGNDFVSLNCSALSPGLLESELFGHKKGSFRGATR
                     DHRGYFQRASGGTLFLDEITEMNAGLQAKLLRVLESNEIRPLGGEKDIAVDARIITAT
                     NIDPEEAVERGRLREDLYYRLAQFPIRVPALRERDNDALLLADHFLKEQNAMTGQNKT
                     FSSRARKALQAYDWPGNVRELKTAVLHSHLLAGSEIKLEDLPGR"
BASE COUNT          654 a          922 c          918 g          560 t
ORIGIN      
        1 ctgttgctga tcgtttccgg cctggttggc cggcgccgcc tgctcagtgc ccttacgccc
       61 atagccgacc tgcagaagcc gattgccgaa cttgcaaagg gcaatctgtc cgtccagttc
      121 aagccagcgg agcaccggga aatatcggac atcgtggagg ccctggagtc gacagcgtcg
      181 gcgttaagcg agcgcaatga gaaactgtcc aggcttgcca accacgacgt cctcaccgga
      241 cttttcaatc ggcgccgatt cgtcgaggag ttgcgcaagg aagttcgaaa tgtcgcgcga
      301 gagcgaaaca ctgccgcgct gctgttcgtc gacctcgacc agttcaagta cgttaacgat
      361 acctgcggcc atccggcggg cgaccggttg atcaggaagg tcgcggatca gctgcagaga
      421 tgcgtgggtg ccagcgggac cgtcggcagg ttcggtggag acgagttcac cgtactcgca
      481 acccgtgttt cgaagccgga ggcacaggcg ctagccgagt cgatactgga agacctgcgc
      541 aaggtggcgc acgtcgagga cgacaacgtg ttccacgtgc attgcagcat cggcgtcgcg
      601 atgattgatt ccgaccgctt cgatcatgac gatctcattt cacaggcgga catagcctgt
      661 cgtgaggcga aagcttcggg aaggaaccgt cttgccttcc acagcatgtc cgacaatgac
      721 gtcgagcagg tggcggccga cgtcagctgg gtcagcaaac tgcgggaggc aatcgacaat
      781 aacctgctta tgctgcgcta ccagcctatc gtatgcattg cgaccggtga gaccacgcat
      841 cacgaagtgc tgctacggat gagatctgac aacggcgaca tggtatcgcc gaacgccttt
      901 ctcccggctg cggtgcgctt cgggctgatg gccgaaatag atacctggct catcgagacg
      961 gccatagccg agcttgcaag gtaccggcag gatgcgccgg accttcgctt tgcactcaat
     1021 ctttccgcca acgcgttcga agcggaaaac ctgacggcgt ttgtcatgtc gcaactggcg
     1081 cggcagaacg ttcctccgga gtgcgtgacc tttgagatca ccgagagcct ggcgatgcgg
     1141 cacctgaacc acgtagagaa gcagatagca ggactgcgtg agatcggctg ccaggtctcc
     1201 ctcgacgact tcggcacagg ctacagttcc ttcagttacc tgcagaaact gccggtggac
     1261 tacatcaaga tcgacggcag cttcatcagg gacctggtcc gcaacccggt ggaccagaag
     1321 atggtgagga tgatcggaga gatcggcaag gccgccggca tgaaaaccat tgccgagtac
     1381 gtccagtcgg gtgctgcgtt cacactgctt gggcgcctgg gtatcgattt cgcgcagggc
     1441 ttctacatcg ggaggccggc cgccacccct atccgcaagt ccatgcccgt gccgattcag
     1501 tcgcacaagg agcagaagcg aaaacaccag cggaaacgaa atacctcctg aagaggactg
     1561 caagttctcc cgtccggccc tccggcaccg tctgaatttc atgctgtaag tctgcggctt
     1621 ttgtaatttt ttccactcgg ttcctgcgac agcagttgca gatccgaggg gatgtcaccc
     1681 ccgaagcggg ctcctgttca taacgccggc gagtcttgat ggaagaagcg ccgggcaaac
     1741 cagctgttga tcctctggca cacttgttgc atcgccatgt acagcaggtt catgcgcaag
     1801 cctcgcgaac tgttcaggct cgaatccggg caatgacaac agtctctttg gaagttgcgt
     1861 tcgccgtcgc cgccaggggc accctgatgc agagcatgag taactgaagg agcgatattc
     1921 gtgccgcatg cactcatcgt cgacaacgac gtcgaaaatt cacagacgct gtcgcaggtc
     1981 tttcgcgagt gcggatacct gcccgagacc gtcgaaagcc tgccgcaggc cagagaagcg
     2041 ctgctgcgcc acacgcccga gatcgcgatc ctgaatgagc agctcgccgg tgatgacacc
     2101 ctggggctgc tcgaatcgat aaacctcgcg ccggtaacgg aaatctacct catgagcgac
     2161 cagccaaatc caaggataac gccgcgtgcg atccgcatcg gcgcgtccga tttcttcagg
     2221 aaaccggtca acaaggatcg gcttgcgcgc aatctgcgcg atctttcgac ggagttgtct
     2281 tccacgacac cggcggccgg cgcgccttcg ccacgcggcg atgcgctggt cggtgagtcc
     2341 aaggccatgc ggcgtttgta ccggttgcta cgcaaatgcg cgccgggcga cgccagcatc
     2401 tttctcgccg gcgaaagcgg gtccggcaag gaactggtgg cgcgaaccat tcaccagctg
     2461 agcgaccgcg ccggcaacga cttcgtctcc ctgaactgca gcgcactgtc ccccggcctt
     2521 ctcgaaagcg aactgttcgg tcacaagaag ggcagtttca ggggagcgac gcgcgaccat
     2581 cgcggatact tccagcgcgc ctccggcggc accctgttcc tcgatgaaat cactgagatg
     2641 aatgccggtc tgcaggcaaa gctgctcagg gtgctcgagt cgaacgagat caggcctctc
     2701 ggaggggaga aggacatcgc ggtcgacgcg cgcattatca ccgccacgaa catcgaccct
     2761 gaagaggcgg tggagcgcgg tcgcctgcgc gaagaccttt actatcgcct cgcgcagttc
     2821 cccatacgag taccggcact gcgcgaacgt gacaacgacg ctttgctgct ggctgaccat
     2881 ttcctgaaag agcagaacgc gatgacgggc cagaacaaga ccttcagcag ccgcgcgcgc
     2941 aaggccctgc aggcctacga ctggcccggg aacgtaaggg aactcaaaac cgcggtgctg
     3001 cacagccatc tccttgccgg cagtgaaatc aagctggaag accttccggg gagg
//
LOCUS       DASUOM010000274         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1332322,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000274 DASUOM010000000
VERSION     DASUOM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332322"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFY27_05755"
     CDS             <1..314
                     /locus_tag="VFY27_05755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5765053.1"
                     /translation="NKDTLVVTQAILGDAWKGEVPENSLTPSETVESLSRKGVMFSAS
                     LPGDHKSQLLTLEGEDGDEHWVGFHNFFVITRYNRSVMYALAVHQLGQEIASKVESGA
                     S"
     gene            304..1125
                     /locus_tag="VFY27_05760"
     CDS             304..1125
                     /locus_tag="VFY27_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5765054.1"
                     /translation="MLRERVLLAAACVLTGTLAGCATSRPPTDVPPAAQLPASLPTEP
                     IPRQEPRSRYGNGPYYQVLGQRYEVMKSETGYSERGVASWYGKKFHGKATSSRETYDM
                     YAMTAAHKTLPLPSYVRVTNLRNSKSVIVRVNDRGPFVSNRIIDLSYAAAERLDMIRD
                     GTTLVEVTAIGYEELESDEPARQVRLDSPAEILPPDASIYVQVGAFGDAENARRRHSM
                     LRDSGIGTAFVHEDAAGSRALYRVRIGPIANVSEYDSIVEQLQRLGISETHLVTE"
     gene            1169..2356
                     /locus_tag="VFY27_05765"
     CDS             1169..2356
                     /locus_tag="VFY27_05765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5765055.1"
                     /translation="MPDYFLRRSPELMQSVFRLLQTVVLLALASLPSLTKAATLPVPA
                     PPVIGAKSYMLLDGNSGYELAAHEPDVQLAPASLTKIMSAYAIFKALDEGQITLEDEV
                     TISEKAWRTPGSRMFIEVGKRVAVKDLVMGMIVQSGNDASVALAEHVAGTESVFAEVM
                     NRYAIELGMNDSHFMNATGLPHEDHYSTARDMATVARAMINEFPEYYGWYSLPEFTYN
                     DITQTNRNRLLRRDSSVDGMKTGHTIDAGYCLIASAERDGMRIISVVLGTASPKTRTD
                     GSQALLNYGFRFYETRLLYRAGETVTEAKIWKSAWEKTALGLPEDLYITVPRGSYDEV
                     ESVLNMPSILVAPVAQGQPLAELEVSLNGSNLVTAPLRALQENPAGSMWQRGRDAVML
                     MLE"
BASE COUNT          520 a          790 c          766 g          427 t
ORIGIN      
        1 ggaacaagga cacattggta gtgacgcagg ccattctcgg cgatgcctgg aagggtgagg
       61 tacccgaaaa ttccctcaca ccaagcgaaa ctgtcgagtc gctgagccgc aagggcgtga
      121 tgttttccgc atccctgccg ggggatcaca agagccagtt gctgacgctc gagggcgagg
      181 acggcgacga acactgggtc ggctttcaca atttcttcgt catcacccgc tacaaccgca
      241 gcgtgatgta tgcgctcgcg gtacaccagc tcggacagga aatcgcatcg aaggtggaaa
      301 gcggtgcttc gtgagcgcgt gctgctggca gcagcctgcg tcctgacggg gaccctcgcc
      361 ggctgcgcca ccagccggcc tccaacggac gtcccccccg ccgcacagtt gccggcgtcg
      421 ctccctacgg aaccgatccc gcgccaggag ccgagaagcc gctatggcaa cggcccctat
      481 taccaggtgc tggggcagcg atacgaggtc atgaagagcg aaaccggcta cagcgaacgc
      541 ggagtggcgt cctggtacgg gaagaagttc cacggcaaag ccacgtccag ccgcgaaact
      601 tacgatatgt atgcaatgac cgcggcgcat aagacactgc cgctaccgag ttacgttcgg
      661 gtcaccaacc tgcgcaacag caagagcgtc atcgtccgcg tcaacgatcg tggcccgttc
      721 gtctcgaacc gcattatcga cctgtcgtat gccgccgcgg agcgcctcga catgattcgc
      781 gacggtacca ccctggtcga agtgacggcg atcggctacg aggaactgga gagcgacgag
      841 ccggcgcggc aagtccggct ggatagcccc gcggagatcc tgccgcctga cgcgagcatc
      901 tacgtgcaag tgggtgcctt cggtgacgcg gagaacgcgc gccgccggca ctcgatgttg
      961 cgggacagcg gcatcggcac tgcatttgta cacgaagatg ctgctgggtc gcgcgccttg
     1021 taccgggtgc gtatcgggcc catcgcaaac gtcagcgagt acgactcgat cgtggaacag
     1081 ctccagcggc tgggtatcag cgagacccac ctggtcacgg agtagctcgc cgagtgaaat
     1141 gacccgtttt ttccgacgga acgccgacat gcccgactac tttttgcgcc gctctcccga
     1201 actcatgcaa tccgttttcc gactcttaca aaccgttgtt ctactggcgc tggcctcatt
     1261 accctcactc acgaaggcgg cgaccctccc ggtgccggcg cctccggtca tcggggcgaa
     1321 aagctacatg ctcctcgacg gcaactccgg ttacgaactt gccgcgcacg aacccgacgt
     1381 acagctggca cctgcgagcc tgacgaagat catgagcgca tatgccatat tcaaggcgct
     1441 cgacgaggga cagatcacgc tggaagacga agtgaccatc agcgagaaag catggcgcac
     1501 gccgggttcc aggatgttca tcgaggtcgg aaaacgcgtc gcggtgaagg atctcgtcat
     1561 gggaatgatc gtccagtccg gcaatgacgc gagcgttgcc ctggcggaac acgtcgccgg
     1621 caccgagtcg gtgttcgccg aagtgatgaa ccgctacgca atcgagctgg gcatgaacga
     1681 cagccatttc atgaacgcta ccggcctgcc ccacgaggac cactactcca ccgcgcgtga
     1741 catggcgacg gtcgcacggg cgatgatcaa cgagttcccc gagtactacg gctggtactc
     1801 gctcccggaa ttcacgtaca acgacattac gcagaccaac cgcaacaggc tgctgcggcg
     1861 cgactccagc gtggacggca tgaagaccgg ccataccatt gacgcaggct actgccttat
     1921 tgcctccgcc gaacgcgacg gcatgcgcat catctccgtc gtgctcggca ccgccagccc
     1981 caagactcgc acggacggca gccaggcgct cctgaactac ggcttccgct tttacgagac
     2041 ccggctgctg tacagggcgg gtgagacggt cacggaagcc aagatctgga agtcggcatg
     2101 ggaaaagacg gcgcttggcc tgcccgaaga cctctacatc acggtgccgc ggggaagcta
     2161 tgacgaagtg gaatccgtgc tcaacatgcc gtccatactg gtagccccgg ttgcccaggg
     2221 gcagcctctt gcagaactgg aggtgagcct caacggctcc aacctcgtca ccgcgccgct
     2281 ccgcgccctg caggagaatc ccgcggggtc gatgtggcag cgcggccggg atgcggtcat
     2341 gctgatgctg gaatgagcct cgattaagga gataatacct taagccttac ggtgcctccc
     2401 gaggtcgttc ttcaaccggc atcaatcatg aacggtctga ggtggtgccg gcggagcttc
     2461 gcccggggtg ggggggcttt tcaggaacgc cgtgaaccca tcc
//
LOCUS       DASUOM010000275         7540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_621003,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000275 DASUOM010000000
VERSION     DASUOM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621003"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFY27_05770"
     CDS             <1..440
                     /locus_tag="VFY27_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5765056.1"
                     /translation="PTHGEDESARVDRLIVEQAQAIAASVDGVLHLLHTYQPLKGIGA
                     EATRTFKPIRLPVESLSERIAAEHREKLDALAASAGIGKSHTHQLPGAARDVIPYVAR
                     DRKADLVVMGAVARWSGKRSAIGSTAERVLDHLPCDILIVRPG"
     gene            complement(484..2583)
                     /locus_tag="VFY27_05775"
     CDS             complement(484..2583)
                     /locus_tag="VFY27_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5765057.1"
                     /translation="MKHLIMSIVVSAFVVACSAESPETPMLAKIPEKLEIHGDVRIDN
                     YFWLNDRSNPDVIDYLEAENAYADEVMAPFEGLKSTLLDELKSRIKQDDQSVPYRDGD
                     YYYYYRYEEGREYPIYCRRKGSLDADEEILLDVNAGAEGNEFYSVRGFAVSPDHRLAT
                     YGVDTQGRRFYTLHFLDLETGKLLPRRIEKVTADVEWANDSKTILFTRQDPETLRSYQ
                     VYRHTLGEDGDQLVYQEDNETNSIFLERSLTGKFLFLTSAETVSTEVRYLPADAPFGD
                     FRVFLPRQEGHEYYVTDGEDRFYILSNDGATNFQVLEAPLDDTARESWKVLVPHRDDV
                     LVEGFDVLKNHIVVEVLHNGLTQLEVVNRESGHSYRIEFDEPVYTAYADDNYVYDTTW
                     LRYVYESMTTPESTYDFDLDTRQHKLIHEEEVLGGFDRNNYETERVFATARDGTAVPV
                     SLVYRKGMEKNGRNPLLQYGYGSYGATIDPDFDPDVLSLLDRGFIFAIAHIRGGSLMG
                     RDWYYDGRQLNKKNTFNDFIDVSHYLIEQGYTSPAHLYANGGSAGGLLMGAVVNMAPE
                     LYHGVATRVPFVDVVTTMLDASIPLTAGEWNEWGNPADKEFYDYIKSYSPYDNVTEQD
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDSNLLLLKTDMQAGHSGKTGRFQSLE
                     DTALYYAFFLGLEGIRE"
     gene            2810..4132
                     /locus_tag="VFY27_05780"
     CDS             2810..4132
                     /locus_tag="VFY27_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5765058.1"
                     /translation="MRDTTTNLDGKRQLLRGIGFIGVALIALNSVIGAGIFALPAEVA
                     ARAGILSPWLFLACGLLIITVVLTYSELASYFRESGGPVLYTTAAFGPLVGFSTGWVL
                     FLSRMTAFAANANVMALYIGAIWPWFEEGPGRTAIIVAVTVVLTYVNYRGVKDGVRTI
                     GFFTLFKIAPLLLLILLGLPYVSGTTLFPAELPTIDDLGGTTLLLIYAFVGFEAATIT
                     AGETERPRYNLPRALVTTIIGTGILYFLIVLVYVSVLPAAGDSGATLVDVGRVLAGPV
                     GAFVITAAAVFSIGGNLASIMITVPRLTFALANERMLPQWFGAVHEKHATPANSILFL
                     GAMSLVFALSGSFAWLAAASALTRLIVYVLCIAALPVIRSRADDEARSRAFHLKGGYT
                     LPLIALILCIWIAMQSTTKSWQVTGVLLLTGLALYWLATFQARHGDRG"
     gene            complement(4181..5275)
                     /locus_tag="VFY27_05785"
     CDS             complement(4181..5275)
                     /locus_tag="VFY27_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5765059.1"
                     /translation="MRDGRSEGERNPDVSRVLVQPRRPSFADSLRFLASIFRRYDLAI
                     STSTTDRSFINLWFAAPRRIGKIASPGFKTWWKRKIVRAALLVDPDTHVLVENLRFAD
                     LLGIERHYPWESPQSALAPAANNSAVEQLSEPYAVVHLMPGSVLRQWPDEHWRSVIDA
                     LRNRGIAVVATGSGDPAEVAYVDKILTGCSGGEELAPLSSLAGELQFNELAALIQRAV
                     LFIGPDTSVSHMAATTGVPVVALFGPTDVARWGPWPSKWPSEPGRDNSPWQRDLLEQT
                     QGNVSILRMACDCGSRTPDCRLTPSTRASCMTRLAPERVLEAVGRLLQRPETSCVLAE
                     VATKEPEKAAMPLELLSGNRPAGRKPVQSW"
     gene            complement(5398..7125)
                     /locus_tag="VFY27_05790"
     CDS             complement(5398..7125)
                     /locus_tag="VFY27_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5765060.1"
                     /translation="MINSRLTWCSLMLAMTLTGCGGEEPPGAPADEPAVRGTPGGEMA
                     GTNPAKEATSEITADLMRGYVIELSDDKYEGRGPGSPGDEAARAYLIAELQKLGLEPG
                     GPNGSWQQPFDLVGVTTEPPDQWTFRHDEGEVTLKKWDQFIASSGVQQEQAKIEDAEL
                     VFVGYGIQAPEYEWDDFKGQDLKGKVLVILNNDPAWDPDLFAGETRLWYGRWDYKYLS
                     AARQGAAGAIIIHTVPSAGYPFQVVQTSWTGEQFELPAGDEPRNQVNAWVTEDAAREL
                     FELAGQDLDELRTAADNRDFQPVPLGLTTSISMDVTLNKTSSANVLGLIPGSDPELRD
                     EAVIYTAHHDHLGIGTPNEEGDRIYNGAMDNAAGVAQVLAIAKAIKKLPEAPRRSILV
                     ALVGAEEQGLLGSKYYAANPTFPPGKIAANLNYDGGNIWGHTHDVTYIGLGKSSIDEI
                     VEGIAKEQGRVVKPDQFADRGFFYRSDQFSFAKIGVPAMYLDTGTDFVDRPEGWGKEQ
                     VNHYTDVNYHQPSDEYDDSWNFDGMIADALLGYWAGLAIANAEEMPAWNEGDEFEAAR
                     LEALAAEDD"
BASE COUNT         1367 a         2304 c         2273 g         1596 t
ORIGIN      
        1 acccgacgca cggggaggat gaaagtgcgc gcgtagaccg cctgattgtg gagcaggccc
       61 aggccattgc cgccagcgtc gacggagtcc ttcacctgct gcacacatac cagccgctga
      121 agggcatagg cgccgaggcc acacgcacgt tcaagccgat tcgccttccc gtggagagct
      181 tgagcgaacg tatcgcggcg gaacaccggg aaaagctcga cgcgctggca gcatcggccg
      241 gtatcggcaa gagccacacc catcagctgc ccggggcagc gcgggacgtt attccttacg
      301 tcgcgcggga caggaaggcg gacctggtgg tcatgggcgc agtggcgcgc tggagcggca
      361 agcgatcagc gatcggcagc accgcggaaa gagtgctgga tcaccttccc tgcgacatcc
      421 tgatcgtgag gccgggttag cgaggaatac agcagcccgc tatgcgggcg acagtcgaag
      481 cggctattcc cggatcccct ccagtcccag gaagaacgcg tagtaaagcg cggtgtcctc
      541 gaggctctgg aagcgacccg tcttgccgct gtgtcccgcc tgcatatccg tcttcaggag
      601 cagcaggttg ctgtccgttt tcagctcacg aagcttggcg acccatttcg ccggctccca
      661 gtactgcacc tgcgagtcgt gcagtcccgt tgtcacgagc aggtgcgggt aatcctgctc
      721 cgttacgttg tcgtacggcg aataggactt gatgtagtcg tagaattcct tgtcggctgg
      781 attgccccat tcgttccact cgccagccgt cagcgggatg ctggcgtcga gcatagtcgt
      841 gacgacgtct acgaatggca cacgcgtcgc aactccgtga tagagctccg gcgccatgtt
      901 tacgaccgcg cccatcagca atccgccggc actgccgcca ttcgcataca ggtgcgcggg
      961 tgaggtgtac ccctgctcaa tcagatagtg actgacgtcg atgaagtcgt tgaacgtgtt
     1021 tttcttgttg agctggcggc cgtcgtagta ccagtcgcgg cccatcagtg agccgccgcg
     1081 gatgtgcgct atcgcgaaga tgaagccgcg atccagcagg ctgaggacgt cagggtcaaa
     1141 gtcgggatcg atggtcgcgc cgtaagagcc gtacccatat tgcagcaacg ggttgcggcc
     1201 gttcttctcc atgcctttcc ggtagaccag ggacaccggc accgcggtcc cgtctcgagc
     1261 cgttgcgaac acccgctcgg tttcatagtt gttacgatcg aaccctccta gcacctcttc
     1321 ttcgtgaatc agcttgtgct gtcgagtgtc gaggtcgaag tcgtaggtcg attccggtgt
     1381 tgtcatcgac tcatagacat accgaagcca ggtggtgtcg tacacgtagt tgtcgtctgc
     1441 ataggcggtg tagaccggct cgtcgaactc gatccggtat gaatggcccg actcacggtt
     1501 caccacctcg agctgcgtca gcccgttgtg caggacttcg acaacgatgt gattcttcag
     1561 tacgtcgaat ccctcgacca gaacgtcgtc gcgatgcggc acgagcacct tccagctttc
     1621 ccgggcggta tcatcaagcg gcgcctccag gacctggaag ttggttgctc cgtcattgct
     1681 gaggatgtag aagcggtctt cgccgtcggt tacgtagtat tcgtgtcctt cctggcgcgg
     1741 caggaagacg cggaagtccc cgaacggcgc atccgccgga aggtaacgca cttccgtgga
     1801 aaccgtctcc gcgctcgtca ggaacaggaa ctttccggtg agcgatcttt ccaggaagat
     1861 tgagttcgtt tcgttgtctt cctggtagac gagttgatcg ccgtcctcgc ccaacgtgtg
     1921 gcggtagacc tggtacgacc gcagcgtttc cggatcctgc ctggtgaaga ggatcgtctt
     1981 gctgtcgttg gcccattcga cgtcggcggt gaccttctcg atacggcgtg ggagcaactt
     2041 tcccgtttcc aggtccagga aatgcagcgt atagaagcgc cgtccctggg tgtcgactcc
     2101 ataagtcgcc agcctgtgat cgggactgac tgcgaagccc cgcacggaat agaactcgtt
     2161 gccctcggcg ccggcattga cgtcgagcag gatttcctcg tctgcatcga gcgaaccttt
     2221 cctgcggcag taaatcgggt actcccgccc ctcctcatag cggtagtaat agtagtagtc
     2281 gccgtctcgg tacgggaccg actggtcgtc ctgcttgatg cgcgacttca gctcgtcgag
     2341 gagcgtgctc ttcaaccctt cgaagggagc catcacctca tccgcgtaag cgttctcggc
     2401 ctcgaggtaa tcgatgacgt cggggttgct tctgtcgttc agccagaagt agttatcgat
     2461 gcgcacatcg ccatgaattt ccagcttttc aggtattttc gcgagcatag gtgtttcagg
     2521 gctctcggcc gagcatgcga cgacaaatgc tgaaacgaca atcgacatta tcaggtgttt
     2581 catgagctcc cggcccagga caggacataa ccagcgagca ttatcgatgg tttatgtgca
     2641 atgtccaccg cgattgcata gcgtgcacag gcatgagagg tttctccgga tattgcgctg
     2701 gcggcagcgg gttcaagcac ttgacaacgg ctcgaggaga ggcgcatggc gggttcgtcc
     2761 cggtgatgta ctatgcgggc gcggtcaaca acggagttcg ggggagcgtg tgcgggatac
     2821 gacaaccaat cttgacggga agcgtcaact ccttcgcgga atcggcttta tcggcgttgc
     2881 gctgatcgcg ctcaactcgg tcatcggcgc agggatattc gcactaccgg ccgaagtcgc
     2941 agcgcgggcc ggcatcctca gcccctggct gttcctcgct tgcgggctcc tgatcatcac
     3001 tgtagttctc acctactccg agctcgcgag ctattttcgc gagtcgggcg gcccggtcct
     3061 gtacacgacc gcggcgttcg gacccctggt cggcttctcg accggctggg tgctgttcct
     3121 gagccgaatg acggctttcg ccgcaaatgc gaatgtgatg gccctttaca tcggcgctat
     3181 atggccgtgg ttcgaagagg gccccggccg cactgcgatc atcgttgcgg ttacggttgt
     3241 actgacgtac gtgaattacc gcggtgtaaa ggacggcgtc aggacgatcg gcttctttac
     3301 gctgttcaag atcgcgccgc tgctcctgct catcctgctg ggtttgccgt acgtcagcgg
     3361 tacgacgctg ttcccggccg aactcccgac gatcgacgac ctgggcggca cgacgctgtt
     3421 gctgatctac gcgttcgtcg gtttcgaggc tgccacgatc accgccgggg aaaccgagag
     3481 accgcgctac aacctgccga gagctttggt tacaacgatc attggcaccg ggatcctgta
     3541 tttcctgatt gtgctcgttt atgtatcggt gctgccggca gccggggata gcggagcaac
     3601 gctcgtcgac gtgggccgcg tacttgccgg accggttggc gcgttcgtga tcacggccgc
     3661 cgctgtgttc tccataggcg gaaacctcgc gagcatcatg atcacggtgc cgaggctgac
     3721 cttcgcgctg gcaaacgaac ggatgctacc ccagtggttt ggtgcggtgc acgagaagca
     3781 cgcgacgccg gcgaattcga tattgtttct cggcgcgatg tcgctggtgt ttgcgctttc
     3841 cggctcgttc gcgtggctgg ccgcagcaag cgcactgacg cgtctgatag tgtacgtgct
     3901 ctgtatcgct gcgctgccgg ttatccgcag cagggccgat gacgaagccc ggtcccgcgc
     3961 gtttcacctg aagggcggct acacacttcc gctgatcgca ttgatcctct gcatctggat
     4021 tgcaatgcag tcgacaacga aatcgtggca ggtcacaggt gtgctcttgc ttacggggct
     4081 tgcgctttac tggctcgcaa ccttccaggc aagacacggt gaccggggtt aaagcccccg
     4141 ctacaaccgg cgcggcgacc ggctccgcac cgacctcgca ttaccagctt tgcacgggct
     4201 tccgacctgc cggcctgttg cctgaaagga gctccaatgg catcgccgcc ttttctggct
     4261 ccttcgtggc gacctcggca agaacgcaag aggtttccgg gcgttggagg aggcggccga
     4321 ctgcttcgag cacccgctcc ggagccagcc gggtcatgca cgaggctctg gtggacggtg
     4381 tcagcctgca gtcgggcgtt cgcgagccac aatcgcaggc catacgcaat atgctcacgt
     4441 ttccctgcgt ctgctccaac agatcccgct gccaaggcga gttgtcgcgg cctggctccg
     4501 atggccattt cgacggccaa ggtccccacc gtgccacatc agtcggcccg aacaatgcaa
     4561 caacggggac gcccgtggtc gccgccatgt gactcaccga tgtgtcgggt ccgatgaaga
     4621 ggaccgcgcg ctgtatcagt gcggcaagct cgttgaactg cagctcgccg gcgaggctgc
     4681 taagaggtgc cagctcttct cctccggagc atccggtaag gattttgtcg acgtaagcca
     4741 cctcggccgg atccccgctg cctgtcgcca cgaccgcaat gccgcgattc ctgagcgcat
     4801 cgatgacgct tcgccagtgt tcgtccggcc actgcctcag gacggagccg ggcatcaggt
     4861 gaacgacggc ataaggctcg gaaagctgtt ccactgcgct attgttggct gccggcgcaa
     4921 gagctgattg cggcgactcc caaggataat gccgctcgat gcccaacaga tccgcaaagc
     4981 gaaggttttc caccagcaca tgggtgtcgg gatcgaccag cagtgcagcc cggacaattt
     5041 tccgtttcca ccacgttttg aatcccgggg aggcgatttt gccgatccgg cgcggcgctg
     5101 caaaccacag gttgatgaac gagcgatcgg tagtcgacgt cgagatcgca aggtcgtagc
     5161 gccgaaagat gcttgccagg aatctcaggc tgtcggcgaa cgagggcctc cttggctgta
     5221 ccaggacgcg agacacgtcc gggttgcgtt caccctcaga ccttccgtct ctcaccaaca
     5281 agtcgatgcg tgcaccaggc caggcgcatc gcaacgaacg cagaaggggc gcgacgagaa
     5341 ttacgtctcc aatgcccgga acagcgatta caagaattcg ctccatactc catccggtca
     5401 gtcgtcttct gctgcgagag cttccagccg ggccgcttcg aattcgtcgc cttcgttcca
     5461 cgccggcatt tcctccgcgt ttgcgatggc gagtccggcc cagtacccga gcagcgcatc
     5521 ggcgatcatg ccgtcgaagt tccagctgtc gtcgtactcg tcactgggct ggtgatagtt
     5581 gacgtccgta tagtgattca cttgctcctt gccccagcct tccggacggt cgacgaagtc
     5641 agtcccggtg tcgaggtaca tcgccggcac gccgatcttc gcgaagctga actggtcgga
     5701 gcgataaaag aaaccccgat cggcgaactg gtctggcttc accacccgcc cctgctcctt
     5761 cgcaattcct tcgacgatct cgtcaatgga tgatttgccg aggccgatgt acgtcacgtc
     5821 gtgggtatga ccccatatgt tgccgccgtc atagttcagg ttggccgcaa tcttcccagg
     5881 cgggaacgtc gggttcgcgg cgtagtactt ggagcccagc aggccttgct cttccgcacc
     5941 gaccagcgcg accaggatgg agcgacgagg cgcctcgggc aacttcttga tcgcctttgc
     6001 aatcgccaat acctgtgcta cgcctgcagc gttatccatt gcaccgttgt agatcctgtc
     6061 tccctcctcg ttcggcgttc cgataccgag atggtcgtgg tgagccgtgt agatcaccgc
     6121 ctcatcgcgc agttcgggat cgctacccgg gatcaacccg agaacgttcg cggaggaagt
     6181 tttgttcagc gtcacatcca tcgaaataga tgtcgtgaga cctaatggca caggctggaa
     6241 atcgcgattg tcggccgcag tgcgcagttc atcgaggtcc tgtccggcga gttcgaaaag
     6301 ttcgcgggcc gcgtcttcgg tgacccatgc attcacctgg ttgcgcggct cgtcgccagc
     6361 gggcagttcg aactgctcgc cggtccacga ggtctgcacg acctggaacg gataacccgc
     6421 tgacggcacc gtgtgaatga tgatcgcgcc ggctgcgcct tggcgagcgg cgctcaggta
     6481 cttgtaatcc cagcgcccgt accagagacg cgtctcaccg gcgaaaagat ccgggtccca
     6541 ggcaggatcg ttgttgagaa tgacaagcac cttgcccttc aggtcctgtc ccttgaaatc
     6601 gtcccactcg tattcgggcg cctggatgcc gtaaccgaca aacaccagtt ccgcgtcctc
     6661 gatcttcgcc tgttcctgct gtactccgct ggaggcgatg aattggtccc atttcttcag
     6721 cgtgacttca ccctcgtcgt ggcgaaaggt ccattggtcg ggaggctctg tcgtgacacc
     6781 gaccaggtcg aatggctgct gccaggaacc gttggggcca cctggctcga ggccaagctt
     6841 ctgcaactcc gcgatgaggt aggctcgcgc cgcttcgtcg ccgggactgc cgggaccacg
     6901 cccttcgtac ttgtcgtcgg aaagctcgat gacgtacccg cgcatgagat ctgccgtgat
     6961 ctcgctcgtc gcttccttgg cgggattcgt accggccatc tcgccgccgg gtgtgccgcg
     7021 taccgcgggc tcatcagcag gggcgcccgg tggctcctcg ccgccgcagc cggtcagggt
     7081 catggccagc atcaagctgc accaggtcag tctggaattt atcactgtcg tcctcttctt
     7141 caggttccga agatgttatg gccagggagc ctctgcacaa gtccggacga atagacgtcg
     7201 tccatgcctt gtccagaggc tccctgacca tcaacactag cacagaagga gcaggctctg
     7261 ccggccgcca cggcgccgtt ggcgcacatc cggctggcag cttgtaattt tgcgcgtgtg
     7321 ctgcgtaaca attaccacgc gtgtttactc ctgttgctgg ccagacgggg gaatcaacgg
     7381 cagcgaccct tgggcatcgt gcgtggcatg caagttgcaa aaggggagtg ttcccgattt
     7441 cttaaagagg ggtctccttg gtcgcagaat ccgcacccag cgtcaacgaa cgccacctgc
     7501 gcacgcccgg gggctgttcg gaagaggatt ccgtttcccc
//
LOCUS       DASUOM010000276         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_988380,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000276 DASUOM010000000
VERSION     DASUOM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988380"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1538)
                     /locus_tag="VFY27_05795"
     CDS             complement(210..1538)
                     /locus_tag="VFY27_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HEX5765061.1"
                     /translation="MKRWVVVLLVLLAVVLLVSPGIVGQLAETSLKDSISWAKSESED
                     FLVTEETFDRGWFTAVGRHRIELKRGDLRSSLLEFSGRSASDRVPVLVVDTRIDHGLV
                     PFTSMSRKSGSLVPALASTVSTMQLDTGDDEIIDVPGTLYTRVGLTGATASRYLLEPG
                     SFEDGAQVEWQGADVSASTDSSRRSLSYEGTVEPLSLVSGNKSLGLKQASFEGDSRYT
                     DFGFTVGAVSVDIDGLAFSDNAGEAVSVGNLELDASSDVVDERVSAKTTMTVKNFSVP
                     GAGDIDVAMDIAANGLDAASVQEILAQVRAARRAQDPEAALQVVYPQIEDDLRSLLSS
                     GAEVRVDRFDVSLPQGKVTTRLHFELPKMKEGGEASWAALLLALTASADLRVPVALME
                     LAQAATPQSGALIAMGILKLDGDSYIVDAQYEKGLLTVNGAPMPIPLPGR"
     gene            1772..2380
                     /locus_tag="VFY27_05800"
     CDS             1772..2380
                     /locus_tag="VFY27_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5765062.1"
                     /translation="MNRLICKVVAAGFMFMPVAHPAFAEGDPERGRVLGYTCLGCHGI
                     EGYRNAYPSYRVPKLGGQKAEALETALRAYKGGSRPHHTMQAQGSSLTEQDIEDVVAW
                     ITLSPPAKDDLDAESVGNHESVKTCVNCHGTAGANVKPAPPVLSGQHRSYLAEALKQY
                     KDGERGNTVMTAFTTGLTEQDIELLADFFAARDGLYTLGDSH"
     gene            2386..3384
                     /locus_tag="VFY27_05805"
     CDS             2386..3384
                     /locus_tag="VFY27_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5765063.1"
                     /translation="MTAAPPDRDVRTTDFEHGVTAIDTEYVRPLLDASHLIVDRGRAA
                     FVDTGVNDSVPLLLKALQQRDIDAGDVNYVFLTHVHLDHAGGAGLLMQHLPNAVALLH
                     PRGARHMTDPGKLVKGSEAVYGKENVKRMYGEILPIEKHRVRVVADNEEVRLGERTLQ
                     CLFTEGHARHHYCLADPASDGVFTGDSFGISYRDFDTASGEFIFPTTTPVHFDPPEAH
                     KAIDRIMEREPARLYLTHYSRVTDLPRLAGDMHQRIDDFVAIAMDHATAIPRTEAMQA
                     AMFSYLKTELGKHGYSVDEQMLHSLLDVDVALNTMGLEHWLDHFERGSHSSRTAHS"
     gene            3399..4388
                     /locus_tag="VFY27_05810"
     CDS             3399..4388
                     /locus_tag="VFY27_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX5765064.1"
                     /translation="MDSFRAYRIDQQDGRIVAGFARLSLDDLTPGDVVIRVSHSTINY
                     KDALAATGKGKILRKHPLVGGIDLAGTVVRSDDERVREGDVVLVNGCGLSETRDGGYA
                     EYARVDGDSIVPVPEGMTAFQAMQIGTAGYTAALAIHRMEQNGQEPARGPIVVTGATG
                     GVGSVAIDMLDARGYEVVAVTGKADRAEYLKSIGARRILVRDQVDLGKRPMEKAEWGG
                     AIDNLGGEVLAWLTRTVSYGGNIASIGLAASMELHTTVVPFILRAICLLGINSVDTPR
                     DLRLDVWRRLGGDLRPRHLDTIGSRTVSLDELPTCFEQYIEGKVTGRIVVEMG"
     gene            4422..5306
                     /gene="prpB"
                     /locus_tag="VFY27_05815"
     CDS             4422..5306
                     /gene="prpB"
                     /locus_tag="VFY27_05815"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008223026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX5765065.1"
                     /translation="MSASAGERFWQALEAERPLQVVGAINAYAALLAERASFRALYLS
                     GAGVANASFGLPDLAMTTLNDVCEDIRRITYVTGLPLLVDADTGWGSAFMIGRTVREM
                     LRAGAAGCHIEDQVSAKRCGHRPGKALVETGEMVDRIKAAVDARPDERFVIMARTDAH
                     AGEGQQAALDRSARYVEAGADMIFAEALNTLDEYREFTAAAGVPVLANLTEFGQTPLF
                     TTGELADAGVRLALYPLSASRAMAAAADEVYRSIRRDGTQKSVVDRMQTRADLYEVLG
                     YHEYEQKLDELFREQELK"
     gene            5345..>5970
                     /locus_tag="VFY27_05820"
     CDS             5345..>5970
                     /locus_tag="VFY27_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5765066.1"
                     /translation="MTANKKTGGLAGVSAGETALCTVGKEGAGLTYRGYDIYDLADNA
                     SFEEVACLLMHGKLPNSGELEEYIDKLKSLRGLPEQLCTVLEMLPADTHPMDVLRSGV
                     SVLGNFEPEGDFSNQSDVADRLLACLPSMLLYWHRFHAEGKRIDVRTKDDSVSGHFLH
                     MLHDSPPSELHRKTLDTTLILYAEHEFNASTFAARVITGTLSDFHSAVT"
BASE COUNT         1176 a         1854 c         1884 g         1056 t
ORIGIN      
        1 aaatgctttg acgggtggcg tgccgcaggc cggttgccca tttcatccac gctgcgaata
       61 tgccctgccg atctgcaggt cggaggcgcc ggcgctgaag ctcctggaag gcgtcgaggt
      121 ggcatgccat cgtgcggaag aactggacct gcgaccttaa gaaacagcgg agcatgaaat
      181 agaagtgcag tagctgcggt ctgcaagttt taccgcccgg gcagcggtat cggcatcggc
      241 gcaccgttga cagtcagcag gcccttctca tactgcgcgt cgacgatata ggaatcgccg
      301 tccagcttca ggatgcccat ggcgatcaat gcgccggatt gcggtgttgc cgcctgcgcg
      361 agttccatca gcgcgacggg cacgcgcagg tccgccgatg ccgtcagcgc cagcagcaaa
      421 gcggcccacg aggcttcgcc gccttccttc atcttcggca gttcgaaatg gagcctggtg
      481 gtcaccttgc cttgcggcag gcttacgtcg aagcgatcga cacgtacttc cgcgcccgag
      541 gacagcagtg accgaagatc gtcttcgatt tgcgggtaca caacttgcag ggccgcctcc
      601 ggatcctgcg cgcgccgggc tgcacgaacc tgcgccagga tctcctgcac ggaagcggca
      661 tccaggccgt tcgcggcgat gtccatcgcc acatcgatgt cgcctgcgcc cggcacggaa
      721 aagtttttga cggtcatggt ggtctttgcg ctgactctct cgtcgacgac gtcgctgctt
      781 gcgtccagtt cgaggtttcc gacgctcacg gcttcgcccg cgttgtcgct gaaagcgagg
      841 ccgtcgatgt ctaccgagac cgctccgacc gtgaagccga agtccgtgta cctgctgtcg
      901 ccctcgaacg aagcctgctt cagcccgagc gatttgttac cggacaccag cgaaaggggt
      961 tcgaccgttc cttcatagct cagactccgc ctggacgagt cggtactcgc cgaaacatcc
     1021 gcaccctgcc attcgacctg cgccccgtct tcgaacgatc cgggttcgag caggtagcgc
     1081 gaagccgtgg ctccggtgag ccctacccgg gtgtagagcg tgcccggaac gtcgatgatt
     1141 tcgtcatcgc cggtgtcgag ctgcatggtt gaaacggtgc tggcaagggc gggaacgagc
     1201 gatccggact tccgtgacat cgaggtaaac ggcaccagcc catggtcgat gcgcgtatcc
     1261 acgacaagta ccggtacccg gtcgctggcg ctgcgtccgc tgaactccag caggctggat
     1321 cgcaaatctc cgcgcttcag ctcgatacga tgccggccaa ctgccgtgaa ccagccgcga
     1381 tcgaacgttt cctcggttac gagaaagtcc tcgctctcgc tcttcgccca gctgatgctg
     1441 tctttcaggc ttgtttcggc gagttgaccc actatgccgg gcgacacaag aaggaccacc
     1501 gccagcagca ccagtagaac caccacccag cgtttcattc tttccctttg gcctggccgg
     1561 ccggagaatc gcggctgccg acgcgaaaat tgaagcgata cgtgcggcca ctctacagaa
     1621 aggcgggaag tgcccgcaac ggcaccatgc gaaacagccg aaccgcgatt taggcgcttg
     1681 gctagaaata agcgcccggt gtcactacca tatcgccctc ggcctgttgc cggatctcaa
     1741 tccgggctca ctcatacctt taaaaaatca cgtgaacaga ctcatttgca aagtcgtcgc
     1801 ggcgggattc atgtttatgc cggtcgcgca tcctgccttc gcggagggcg acccggagcg
     1861 cgggcgagta ctgggctaca cctgcctggg ctgtcacggc atcgagggct atcgcaatgc
     1921 atatccctcc tatcgcgtgc cgaagctcgg cggacagaag gcggaagcgc tggagactgc
     1981 attgcgtgcc tacaagggcg ggagccggcc gcaccatacg atgcaggcgc agggttcgag
     2041 cctgacggaa caggacatcg aggacgtcgt ggcctggatt acgctatcgc cgccggcgaa
     2101 agacgatctc gatgccgagt cagtgggtaa tcacgagtcg gtgaagacct gcgtcaactg
     2161 ccatggaacc gcaggagcca atgtgaagcc ggctccgccg gtactctccg ggcagcaccg
     2221 aagctacctg gcggaagcgc tgaagcagta caaggacggt gaacgtggca acacggtaat
     2281 gaccgcgttc accacggggc ttaccgagca ggacatcgaa ctgctggctg atttcttcgc
     2341 tgcccgggac ggactttaca cgctcggcga ttcccactag cggccatgac cgccgcgccg
     2401 ccggatcgag acgttcgcac gaccgacttc gagcatggtg tcactgcgat agacaccgag
     2461 tatgttcgcc cccttttgga cgccagtcac ctgatcgtcg accggggacg cgcagcattc
     2521 gtcgacaccg gggtaaacga cagcgtcccg ctgttgctca aggctttgca acaaagagat
     2581 atcgacgcag gggacgtaaa ctacgttttc ctgacccacg tgcacctcga ccatgccggt
     2641 ggggccgggc tcctgatgca acacctgcca aacgcggtag ccctgctgca tccacgcggc
     2701 gcgcgccaca tgaccgaccc cggcaagctt gtaaagggtt ccgaggctgt ttacggaaaa
     2761 gagaacgtga agcggatgta tggcgaaatc ctgccgatcg agaaacaccg cgtacgcgtg
     2821 gttgccgaca acgaggaggt gcggctcggc gagcgcacat tgcaatgcct tttcacggag
     2881 gggcacgccc gccaccacta ctgcctggcc gatcctgcat ccgacggcgt gttcaccggc
     2941 gacagtttcg gcatttccta ccgcgacttc gataccgcca gcggcgagtt catcttcccg
     3001 acgacgacgc cggtgcattt cgacccgccg gaggcgcaca aggctatcga ccgcatcatg
     3061 gaacgcgagc ccgcacggct ttacctgacg cactacagcc gggttaccga cttgccgcgc
     3121 ctggcgggcg acatgcacca acggatcgat gacttcgtgg caattgccat ggatcacgct
     3181 actgctatcc cgagaaccga agccatgcag gcagccatgt tctcctacct gaaaaccgaa
     3241 ctcgggaagc acggctacag cgtcgacgag caaatgctgc actcgttgct ggatgtcgac
     3301 gtggcgctca acaccatggg tctcgagcac tggctcgacc actttgaacg cggctcgcac
     3361 tcgagccgta cggctcattc ataaagggac cgacgatcat ggacagcttc cgcgcatatc
     3421 gcatcgacca gcaggacggc aggatcgttg ccggtttcgc caggctctcg ctggacgatc
     3481 tcactcccgg agatgtcgtg atcagggtca gccactccac catcaactac aaggacgcgc
     3541 tggcggctac gggcaaaggc aagatcctcc gcaagcaccc gcttgtcggg ggcatcgatc
     3601 tcgcgggaac cgtggtgcgg tccgatgacg agcgcgtcag ggaaggcgac gtcgtactgg
     3661 tcaacggatg cggcctcagc gagacccgcg acggcgggta cgcggagtat gcacgcgtgg
     3721 acggtgactc catcgtgccg gtcccggagg gcatgactgc gttccaggca atgcagatcg
     3781 gaacggcagg ttacacggcg gcgctggcaa tccaccgcat ggagcaaaac ggccaggagc
     3841 cggcgcgcgg ccccatcgta gtgaccggtg cgacgggcgg cgttggcagc gtcgcgatag
     3901 acatgctgga cgcccgcggc tacgaggtgg tcgcggtaac cgggaaggca gaccgtgcgg
     3961 agtacctgaa gagcatcggg gcgcgaagga tcctcgtgcg cgaccaggtc gatctgggca
     4021 agcgccctat ggagaaagcc gagtggggcg gtgcaataga taatctcggc ggcgaagtgc
     4081 tcgcttggct tacgcgaacg gtcagttatg gcggcaatat cgcgagcata gggcttgccg
     4141 ccagcatgga actgcacacg accgtggtgc ccttcatact ccgcgcgatc tgcctgctgg
     4201 gcatcaattc ggtcgacacg ccccgggacc tgcggctgga tgtgtggcgg cgtttaggag
     4261 gcgatcttcg accgcggcat ctcgatacga tcggcagccg taccgtatcc ctcgatgaac
     4321 tgccgacctg cttcgagcaa tacatcgagg ggaaggtaac cggccgcatc gtcgtcgaga
     4381 tgggctgaac cggccttaca ggattcctga tgggagacgt catgtcagct agcgccggag
     4441 aacgattctg gcaggcactg gaggccgagc ggccgctgca ggtggtggga gcgatcaatg
     4501 cgtacgcggc tcttctcgcc gagcgagcga gctttcgcgc gctgtacctc tcgggtgccg
     4561 gtgtcgcgaa tgcatcattc gggcttcccg accttgccat gaccacgttg aacgacgtct
     4621 gcgaggatat caggcgcatc acctacgtca ccgggctgcc gctgctggtg gacgccgata
     4681 ccggctgggg cagcgcattc atgatcggca ggacggtgcg cgagatgctt cgtgccgggg
     4741 ctgccggctg tcacatcgag gaccaggtgt cggccaagcg ttgcggccac cgccccggca
     4801 aggccctggt agagacgggc gaaatggtcg accgcatcaa ggcggcggtg gatgctcgcc
     4861 ccgacgaacg tttcgtgatc atggcccgca ccgacgcaca cgccggcgaa ggacagcagg
     4921 ctgcgctgga ccgctcggca cgctatgtgg aggccggggc cgacatgata ttcgccgaag
     4981 cgctgaatac tctcgacgaa taccgcgagt tcacggccgc ggcgggcgtg ccggtgctcg
     5041 cgaaccttac ggagttcggg cagacgccgc tattcaccac cggggaactg gcggacgcag
     5101 gcgtgcggct ggccctgtac ccgctttcgg catcgcgcgc gatggcggcc gcggcggacg
     5161 aggtataccg cagcatccgg cgtgacggaa cgcaaaagtc cgtcgtcgac aggatgcaga
     5221 cgcgggccga cctgtacgag gtgcttggtt accacgagta cgagcaaaag ctcgacgaac
     5281 tcttccggga gcaggagttg aagtagttcc gatcctgccc ggtgcagaca aatggagtaa
     5341 ggatatgacg gcgaacaaaa agaccggcgg gctcgcgggt gtctcggccg gcgagacggc
     5401 gctctgcacg gtaggcaagg aaggcgcagg ccttacttac cgcggctacg acatctacga
     5461 cctcgcggac aacgcgagct tcgaggaagt cgcctgcctg ctcatgcacg gcaagttgcc
     5521 caactctggc gagctggagg agtacatcga caagctcaag agcctgcggg ggcttccgga
     5581 acaactgtgc acggtcctgg agatgctgcc tgcggacacg catccgatgg acgtactgcg
     5641 aagcggcgtg tccgtgctcg gcaacttcga gcccgaggga gatttttcga accagagcga
     5701 cgttgccgac cggctgctgg catgcctgcc gtcgatgctt ctctactggc accgtttcca
     5761 tgccgagggc aaacgcatcg acgtccggac gaaagacgac agcgtctcgg gacattttct
     5821 ccacatgctg cacgactcgc cgccgtcgga gctgcacagg aagacgctcg ataccacgct
     5881 tatcctgtac gccgagcacg agttcaatgc ctcgacgttt gcggcccgtg tcatcactgg
     5941 cacgctgtca gattttcact ccgcggtgac
//
LOCUS       DASUOM010000277         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_475330,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000277 DASUOM010000000
VERSION     DASUOM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475330"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..503
                     /locus_tag="VFY27_05825"
     CDS             126..503
                     /locus_tag="VFY27_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5765067.1"
                     /translation="MNFSADAYMHEPVKYPALHVTNLNDEGKKVTGRYRNMVINQVNE
                     SCLRLAVFEGEYRWHYHPSSDELFIVVEGCLAIDLEDGRELRLQPWDAVTVPAKTVHR
                     TRAIGRTINVCFEQVGAETVFIE"
     gene            511..1041
                     /locus_tag="VFY27_05830"
     CDS             511..1041
                     /locus_tag="VFY27_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008218212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5765068.1"
                     /translation="MCAHKEQLRGFARYNSEFNRRLFDQLSALPDEERKRDMGAFFGS
                     IHGTLNHILLADRIWLGRFAATIPAMSSLDGAAVVHEFSSLRQELCAGFDELYAERRA
                     TDQVISCWAEELSDDMLAGTMRYRNSEGNLREHFVWIAVAHMFNHQTHHRGQVTTLMH
                     QLGHDPGVTDYLVYVQ"
     gene            1093..1545
                     /locus_tag="VFY27_05835"
     CDS             1093..1545
                     /locus_tag="VFY27_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765069.1"
                     /translation="MNIRTATVEDAELLMPFVRAYHEFEGIGSTEPERARSVAPLLSD
                     ASLGRVWMILEEGQAVGYIAVCFGYSIEFGGRDAFLDEFFLAPAARSRGLGGKVLEAV
                     KNEVTRLGVRAVHLEVARTNFGAKKFYSKAGFKSRERFHLMSCMLGGD"
     gene            1572..2159
                     /locus_tag="VFY27_05840"
     CDS             1572..2159
                     /locus_tag="VFY27_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765070.1"
                     /translation="MIFGHTMTRYGATVALLSLALVFPFVSASEEPSYAGQELRSIKS
                     LSEQEIESLRRGDGMGFAKLAELNHFPGPRHVLDLSRELELSSRQLADTQALYNEMLE
                     HARSLGAGIVEAETELDRRFELGSITAESLQTALHDIGRLRAELRYVHLRAHLRQREL
                     LSAEQVRKYDALRGYGEALDDHSKHQHSQPVPSTH"
     gene            2233..3153
                     /locus_tag="VFY27_05845"
     CDS             2233..3153
                     /locus_tag="VFY27_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5765071.1"
                     /translation="MSRTPQAEDPKISPARLMALTALTMLAFAGNSLLCRMALEHTGL
                     DPATFTTIRVVSGALTLWMLVQLRRAHRTGSRGNWTSALALFVYAAGFSFAYVSLTAA
                     SGALMLFVAVQATMVGHGLWRGERFRHLQIAGLVLALGGLVGLLLPGLSAPPLGGSIL
                     MLAAGAAWGVYSLRGKGSGDPLAATAGNFLRAVPVALVLSVLAHRQFNFDSSGFWYAV
                     SSGAIASGVGYAIWYTVLPFLKATEAATVQLSVPVIAAVGGIILLDEPMTLRLVIASV
                     AILGGIALVIRKPGAPSAAKRVPAGNSPRR"
     gene            complement(3175..3384)
                     /locus_tag="VFY27_05850"
     CDS             complement(3175..3384)
                     /locus_tag="VFY27_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5765072.1"
                     /translation="MTNRIRQLRLEAGQMTQQALAEQVGVTRQTVIAIENDRYSPSLE
                     VAFRIARVFGAGLEDVFGYDQSPVE"
     gene            complement(3407..3931)
                     /locus_tag="VFY27_05855"
     CDS             complement(3407..3931)
                     /locus_tag="VFY27_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765073.1"
                     /translation="MIYLTLLVGAMQEGRDMSFKEKSIFGTLLLVLWVAAAYFTKVVA
                     MLTAGDVDFVALGKLAVGLIVFFIIVEIVLHTVIAAGSPKEVLEIEDERDRLIELKGE
                     RVGGLLLGIGVAFTIGHIAVHGVFGDRTSSYPEIARFFDNSPFVTANLLLFWLTVSEV
                     GKYVSQLLYYRRGV"
     gene            4015..>4678
                     /locus_tag="VFY27_05860"
     CDS             4015..>4678
                     /locus_tag="VFY27_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5765074.1"
                     /translation="MRIDAVVFDMDGLMLDTEPLYKTAWQGALAELGHELDDAAYLRF
                     VGRSTEDCENDLVSQLGEAFPLEAFRDRWPVLWRTCADEQGIAAKAGLHELIAFVRDE
                     GLQLALATSSDSWFTELSLGCANLLGLFDVVVTGNQVTHPKPAPDIYIEAARRLAVNP
                     RHCVALEDSEAGILAASRAGMVPLLVPDLRKPTEEAIEAAFRVLGSLHEARELLIEQM
                     HNS"
BASE COUNT          888 a         1341 c         1434 g         1015 t
ORIGIN      
        1 tggagttcag ctcgggagcg gtggcgatgc ggtatgaacc ccggaggtag cgcctgtgat
       61 cgcgcgaccc acatgaagcc ccagcttgag tttgctagcc tgatctctga caccggagga
      121 aactcatgaa cttttccgct gacgcctaca tgcacgagcc agtgaagtac cctgcactcc
      181 acgttacgaa cctgaacgat gaaggcaaaa aggtaactgg ccggtatcga aacatggtca
      241 tcaaccaggt aaacgagagc tgcctgaggc tcgcggtttt cgaaggtgag taccgctggc
      301 actaccaccc gtcgtcggat gagctgttca tcgtcgtgga aggatgtctc gccatagatc
      361 tggaggacgg gcgggaactg cgccttcagc cgtgggacgc cgtgacggtg ccggcgaaga
      421 ccgtccatcg cacgcgcgcc atcgggcgaa ccatcaacgt gtgcttcgag caggttggcg
      481 ctgagacggt ctttatcgag tgaggggatc atgtgcgcgc ataaagaaca gcttcgggga
      541 ttcgccaggt acaactccga attcaaccgg aggctttttg accaactctc ggcgttgcca
      601 gatgaggagc ggaaaaggga catgggtgct ttcttcggct ccatccacgg caccctgaat
      661 cacatcctgc ttgcggatcg catctggctg ggccgttttg ccgcgacaat tcctgcaatg
      721 tcgtcactgg acggggcggc cgtggtacac gagttctcat cactgcggca ggagctgtgt
      781 gccgggtttg atgagcttta cgccgagcgg cgtgccaccg accaggtgat atcctgctgg
      841 gctgaagaat tgtcggacga tatgctcgcc ggaacgatga ggtatcgaaa ttctgaagga
      901 aaccttcgtg agcattttgt ctggattgcg gtggcacaca tgttcaatca ccagacgcac
      961 catcgcgggc aggtaacaac cttgatgcat cagctcgggc atgatcccgg tgtgaccgat
     1021 tatcttgttt atgtgcagta acactgtcct ctatgggcac ccaaccgagg cttttttgtc
     1081 actgatcctg caatgaacat acgaactgca acagtcgagg acgccgagct actcatgcct
     1141 ttcgtccgtg cgtaccatga gttcgaaggt atcgggtcga cggagccgga gcgcgccaga
     1201 tccgttgctc ccctgctgtc cgatgctagc ctgggtcgag tctggatgat tctcgaggaa
     1261 ggtcaggcag tcggatatat cgcggtatgc tttggctact ccatcgagtt cggtggccgc
     1321 gatgccttcc tggacgaatt tttcctggca cccgccgcgc gttcacgagg tcttggcggc
     1381 aaggttctgg aggcagtcaa aaatgaagtg acgcggctgg gagtgcgggc cgtgcacctg
     1441 gaagtggcga ggaccaattt cggcgcgaaa aagttctact cgaaggcggg cttcaagtcc
     1501 cgtgagcgct ttcacctgat gtcctgcatg ctcggtggcg actgagtaag gtgggatcgg
     1561 gagaaccatt gatgattttc ggccacacga tgacgcgcta tggggcgaca gtagcattgc
     1621 tgtctcttgc cctggtgttt ccctttgtct ccgcatcgga ggaaccgtca tacgccggtc
     1681 aagagctacg ttcgatcaag tcactgtcgg agcaggagat cgagtcattg cggcgcgggg
     1741 atggaatggg ttttgcgaaa ttggcagaac tgaatcattt ccccgggccc cggcacgtcc
     1801 tggatctttc ccgagagctt gagctgagtt cccggcagct tgccgatacc caggctctgt
     1861 acaacgaaat gctagagcat gccagatcgc tgggtgccgg gattgtcgag gctgaaacgg
     1921 agctcgatcg gagattcgaa ctgggatcga ttactgcgga atcacttcaa acggcgctgc
     1981 atgacatcgg tcgcttgcgg gccgagctca gatatgtgca tctccgggca cacctgcgcc
     2041 agagggagct gttaagtgca gagcaggtcc gcaaatacga tgcattaaga ggatatggcg
     2101 aggcactcga tgatcatagc aagcatcagc actcgcagcc ggtcccctcc acccattgaa
     2161 ctcgtcgttg aacgtttctg cacagccaaa cctgctcgta gctgtttttt cccgaagaac
     2221 cgatccgggt taatgagcag gactccgcag gccgaggatc ccaagatctc tccggcacgg
     2281 ctcatggccc tgacggcgct gaccatgctg gcgtttgccg ggaattccct cctctgtcgc
     2341 atggccctgg aacacaccgg cctcgatccg gcgaccttca cgacgattcg cgtggtgtcg
     2401 ggcgcgctca cgctgtggat gctggtgcag ctgcgtcgcg cccatcgaac cggaagtcgc
     2461 ggtaactgga cgtcggccct tgcgctgttc gtttacgccg ccggtttttc gttcgcctat
     2521 gtcagcctga ccgcggcatc gggtgcgctg atgctgttcg tggcggtcca ggcgacgatg
     2581 gtcggtcacg gtctctggag gggcgaacgc tttcggcact tgcagatcgc tgggctcgtc
     2641 cttgccctgg gtggactcgt cggtctcctt cttccaggtc tctccgctcc cccgcttggc
     2701 ggctcaatcc tgatgctggc cgctggagct gcctggggtg tttattcgtt gcgcggcaaa
     2761 ggctcgggcg accctctcgc agcaaccgcg ggaaacttcc tgcgcgcggt tccggttgcc
     2821 cttgtgctga gcgtcctggc gcatcgccaa ttcaatttcg acagttccgg attctggtat
     2881 gcagtttcgt ccggggcgat tgcctccgga gtgggctacg ccatctggta taccgtattg
     2941 ccttttctca aagccacgga ggccgcgacg gtgcagctga gcgttcctgt tatcgccgcc
     3001 gtgggtggca tcattctcct ggatgagcca atgaccctgc gcctggtcat cgcttctgtc
     3061 gcgattctcg gcggcattgc actggtgatc cggaaaccgg gcgcgccttc cgcggccaaa
     3121 agagttccgg caggaaattc accgcgtcgg tgagtcgcat cgttcgcgcc ggcatcattc
     3181 cacgggactt tggtcgtagc cgaacacgtc ttcgagacct gcgccgaaga ccctggcaat
     3241 gcggaaggcg acttccagcg acggcgagta ccggtcgttt tctatcgcga taaccgtctg
     3301 ccgggtcacg ccgacctgtt ctgccagcgc ctgctgcgtc atttggcccg cctcgagccg
     3361 caattgccgg atgcggttgg tgatgcgggg ttttcccttg cccaagtcag accccgcgcc
     3421 ggtagtacag gagctggctc acgtacttcc cgacttcgga aactgtcagc cagaagagca
     3481 gcaggtttgc ggtgacgaac ggggagttgt cgaagaagcg cgcaatctcg gggtacgagc
     3541 tggtcctgtc gccgaaaacc ccgtggactg cgatgtggcc gatggtgaac gcaacgccta
     3601 tgccgagcag caggccgccc acgcgctccc ccttcagctc gatcaaccgg tcgcgttcgt
     3661 cttcgatctc gagcacttcc tttggcgagc cggccgctat caccgtatgg agcacgatct
     3721 ccacgatgat gaaaaagacg atcaggccga ccgcgagctt gccgagcgca acgaaatcca
     3781 cgtcgccggc cgtgagcatg gcgaccactt tggtgaagta cgcggcggcc acccacagca
     3841 ccagcaacag cgtgccgaat atgctctttt ccttgaacga catgtcacgg ccctcctgca
     3901 ttgcgccgac cagcagtgta aggtaaatca tacagtgtgt aaagtattta ttacatgacg
     3961 gtgcgctgat tctgcgcaga tatactgcac aggtctcttc ctgggacccg ccgcatgcga
     4021 atcgatgctg ttgttttcga tatggacggg ctgatgctcg ataccgagcc gttgtacaag
     4081 acagcctggc agggggcact ggcagaactg ggacatgagc tggatgacgc tgcgtacctg
     4141 cgcttcgtcg gtcgatcgac tgaagactgc gagaacgacc tcgtttctca actgggcgag
     4201 gcttttccgc tggaagcctt ccgggacagg tggccggtgc tgtggcgaac ctgcgcggac
     4261 gaacaaggca tcgctgcgaa ggcaggtttg cacgagctga tcgctttcgt cagggacgaa
     4321 ggccttcagc tggcgctcgc aacctcgagc gactcgtggt ttaccgagct cagcctcggc
     4381 tgcgcgaacc tcctggggtt gttcgacgtc gtggttacag gcaaccaggt gacgcatccg
     4441 aagccggctc cggatatcta tatcgaagcg gcgcgacggc tggcagtcaa tcctcgtcat
     4501 tgcgttgcac tggaagattc cgaggcgggt atcctggcag cgagccgcgc cggcatggtt
     4561 ccgctgctgg tgccggatct caggaagccg accgaggaag caattgaggc tgctttccgc
     4621 gttctcggtt cgctgcacga agcgcgcgag ctgttgattg aacaaatgca taattcgg
//
LOCUS       DASUOM010000278         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_130245,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000278 DASUOM010000000
VERSION     DASUOM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130245"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFY27_05865"
     CDS             complement(<1..611)
                     /locus_tag="VFY27_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX5765075.1"
                     /translation="MSAMPGMVTVFVPRETSAVSLGADDVAAEITRLAAGHDSGIDVK
                     RNGSWGMTWLEPLVEVVVNGERIAYGNVAPENVASLFDAGFLSGGEHPLKVGNTAEIP
                     WLRNQDRWTFRRCGLIDPLSIDEYRANGGFAGFERALKLGPAGTLQEVTVSGLRGRGG
                     AGFPTGIKWKTVADAVADQKYIACNADEGDSGTFSDRMLMEGDP"
     gene            complement(615..1082)
                     /locus_tag="VFY27_05870"
     CDS             complement(615..1082)
                     /locus_tag="VFY27_05870"
                     /inference="COORDINATES: protein motif:HMM:NF004638.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX5765076.1"
                     /translation="MPKTYDPAIGTEIIASFGAKPELLVQILHAFIDRYSYLHEAALR
                     QIADELNISRADIHGVVSFYHDFRTSPPGRKVVKICQAEACQSMGSRELTAHAEKKVG
                     VSLNNTTPDGGVTLEPVYCLGNCACSPAVMIDNKVYGRVDGERFDQLLASRGH"
     gene            complement(1131..1973)
                     /gene="fdhD"
                     /locus_tag="VFY27_05875"
     CDS             complement(1131..1973)
                     /gene="fdhD"
                     /locus_tag="VFY27_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5765077.1"
                     /translation="MSGASLAIDVSKVHGRDQVSQSDSVAVEEPLEIQLCASSAEDSA
                     AKSISITMRTPGHDEELALGFLFTEGIITSREQVASAVHCGPEAPGTGYRNVIRIELN
                     PGMQVDLTRLERHFYTTSSCGVCGKSSLDALRVSGQEPMTGNSTTFRREVIVQLPDKL
                     RGEQEIFATTGGLHAAAAFTPDGEITLVREDVGRHNAVDKVVGALLKANRLPAQEMGL
                     LVSGRASFELMQKALVAGFPLLAAVGAPSSLAVSLAKEFDMTLVGFLRRGNFNIYSHA
                     ERLA"
     gene            complement(2099..3580)
                     /locus_tag="VFY27_05880"
     CDS             complement(2099..3580)
                     /locus_tag="VFY27_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765078.1"
                     /translation="MLLTLCVTGFASAAPPQQYPHRYRITIDPAMSTMAVEAHFSLSV
                     DAVNAKTRDAGEYLADVRDCDSAKLIRLRNQRMMLPAGGIRCMNYTVDLQAAARSTDR
                     DQLLAAGNIVVSPSLWLWRPALATGESIDIRFDLPEGMQVAVPWPRDEREPNAYRLGS
                     SPESSSAPAVFGRFDYREMDVPGATLRVSVPRTRSDKDDRVDSEFIFEWLRRTATDVS
                     LAYGRFPNPSLQVVVLPVDESRRSASAVPYGRVVRDGGEAVELYVDRDQPLDAYLRDW
                     TATHEFSHLMLPYVAPAHRWVSEGFAQYYQNVLLARSGTYDPLEAWQKLYDGLERGRQ
                     SRPELSPNEATARGMHNGRMKIYWSGAAIALMADVTLRERSGGTQTLDDVMERLQFCC
                     LPSERAWSGPELFETLDILAGTPVFMPLYRRYADTAGFPDTRPVFERLGLAVSNDKVR
                     IRRGGELQHIRTSITRADSIPAGREQLAGRKSVARREGAAGSR"
     gene            3845..4165
                     /locus_tag="VFY27_05885"
     CDS             3845..4165
                     /locus_tag="VFY27_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX5765079.1"
                     /translation="MSKIARRQFIQLSAVAAAGCFIRPGTEARAQELVKISEDDPVAQ
                     SLKYTHDASTVDAATRPNPAENQVCANCALIQGEEGEWRPCQIFPGKLINENGWCSVW
                     APKA"
BASE COUNT          745 a         1345 c         1361 g          785 t
ORIGIN      
        1 ggatcgcctt ccatcagcat gcggtcggaa aacgtgccgc tgtcgccctc gtcggcgtta
       61 caggcgatgt acttctggtc agcaacggcg tcggcgacgg ttttccactt gatgccggtg
      121 gggaagccgg caccgccgcg accgcgcagg cccgagaccg tcacttcctg cagggtaccc
      181 gcgggaccga gcttaagggc tctctcgaaa ccagcgaagc cgccgttcgc gcgatactcg
      241 tcgatcgaca gcgggtcgat caggccgcag cggcggaacg tccagcgatc ctggttgcga
      301 agccagggta tttcggcagt gtttcccacc ttgagcggat gttcgccgcc cgagaggaaa
      361 ccggcgtcga acaggctcgc aacgttttcc ggcgcgacgt tgccgtaagc gatccgctcg
      421 ccgttcacca ccacttcgac cagcggctcg agccaggtca tcccccagga gccgttccgc
      481 ttcacatcaa tcccggaatc gtgcccggcc gccagcctcg tgatttcggc ggcgacatca
      541 tccgcgccca gggataccgc cgacgtctcg cgcggtacga agactgtgac catacccggc
      601 atcgcggaca tcgctcagtg cccccggctg gcgagcagct ggtcgaaccg ttcgccgtcg
      661 acgcggccgt acaccttgtt gtcgatcatg acggccggcg agcaggcgca gttacccagg
      721 caatacaccg gttccagcgt caccccccca tcgggggtcg tattgttgag cgatacgccg
      781 accttcttct cggcatgcgc agtgagttcg cggctgccca tggactggca ggcttccgcc
      841 tggcagatct tcactacttt gcgtcccggc ggggaggtgc ggaaatcgtg atagaagctc
      901 accaccccgt ggatgtcggc gcgggaaatg ttcagctcgt ctgcaatctg gcgaagcgcc
      961 gcctcatgca ggtaggagta acgatcgatg aacgcgtgca ggatctgcac cagcagctcg
     1021 ggcttcgcgc cgaagcttgc gatgatttct gtccctatgg cggggtcgta ggttttcggc
     1081 atgttgattc gcgaaatttt ctaaaaaaag tatgaccgcc atggccttgg tcaggccagg
     1141 cgttctgcgt gcgaatagat attgaagtta ccgcggcgca ggaagcctac cagagtcatg
     1201 tcgaactctt tcgccaggct caccgcgagg ctggaaggag cgcccaccgc tgcgagcaac
     1261 gggaagcccg ccaccaacgc cttctgcatt aactcgaagc tggccctgcc tgacaccaag
     1321 agccccattt cctgcgccgg caaccggttc gctttcagca acgcgcccac taccttgtcc
     1381 accgcattgt gccgtccgac atcctccctg acgagcgtga tttcaccatc tggggtgaag
     1441 gcggcggcgg catgaagtcc tccggttgtc gcgaaaattt cctgctcccc gcgcagcttg
     1501 tccggtagct gaacgatcac ttcacgacgg aacgtcgtcg agttgccggt catgggctcc
     1561 tggccgctga cgcgcagcgc gtcgagcgac gatttgccgc agacgccgca actcgaggtc
     1621 gtgtagaagt ggcgttcaag acgggtcagg tcgacctgca taccaggatt tagttcgatg
     1681 cggatgacgt tcctgtagcc ggtgcccggc gcttccggcc cgcaatgcac ggccgaggct
     1741 acctgctccc ggctggtgat aatgccttca gtgaacagga acccgagcgc gagttcttca
     1801 tcgtggcccg gcgtgcgcat cgtgatcgag atgctcttcg cggcggagtc ttctgccgag
     1861 gacgcgcaca actggatttc aagcggttcc tctacggcaa cgctgtcgct ttgtgagacc
     1921 tggtcccgtc cgtggacctt gctgacatcg atggcgaggc tcgcgcctga catgaccgct
     1981 acctttactt gccgggccgc tcattataca tcagggggtg ttgctggagc ccgcccccgg
     2041 ccgggctgca aggacgcgag ctcgcctgca cgcagggtcg tgcaggggcg tcttccggct
     2101 agcgcgagcc ggccgcgccc tcccttcgtg caacggattt gcgtcccgcc agctgctcgc
     2161 ggccagccgg gatgctgtcg gcccgtgtga tcgaggtgcg tatgtgctgc agctcgccgc
     2221 cgcgccggat cctgaccttg tcgttcgaca cggccagccc caggcgctcg aacaccggcc
     2281 gcgtgtccgg aaagcccgcg gtatcggcat agcggcgata cagcggcatg aacacgggag
     2341 tgcccgcgag tatgtcgagg gtttcgaaca gttcgggacc gctccacgcc cgctcggacg
     2401 ggaggcagca gaactgcagc cgctccatga cgtcgtcgag cgtctgggtg ccgccggagc
     2461 gttcccgcag cgtcacatcc gccattaatg cgatcgccgc gccgctccag tagatcttca
     2521 tccgcccgtt gtgcatgccg cgagccgtgg cttcgttggg cgagagctcg ggacgggact
     2581 gccgcccgcg ctcgagccca tcgtagagct tctgccaggc ctcgagcgga tcgtaggtac
     2641 cggagcgcgc cagcagcacg ttctggtagt actgcgcaaa gccttcggac acccagcggt
     2701 gcgccggcgc gacgtaaggc agcatcaggt gactgaattc atgcgtagcg gtccagtcgc
     2761 gcaggtaagc gtccagcggc tggtcacgat cgacgtaaag ctcgacggcc tctccgccgt
     2821 cgcggacgac cctcccgtaa gggaccgcgc tcgcgctgcg gcgggattca tcgacaggca
     2881 gtacgactac ctggagcgag ggattcggga accgcccgta agccaggctg acgtcggttg
     2941 ccgtgcggcg cagccattca aagatgaatt cgctgtctac gcgatcgtct ttgtcgctgc
     3001 gggtgcgcgg cacgctgacg cggagcgtcg cgccgggcac gtccatctcg cgatagtcga
     3061 atcggccgaa caccgccgga gcgctcgaac tctcggggga actgcccagg cgatatgcgt
     3121 tcggttcacg ttcatcgcgt ggccacggca ccgcgacctg catgccttcc ggaagatcga
     3181 agcggatgtc gatcgattcg ccggtcgcca gtgcggggcg ccagagccac agcgacgggg
     3241 ataccacgat gttcccggcc gccagtaact ggtcgcggtc ggtgctgcgg gcggccgcct
     3301 gcagatccac ggtatagttc atgcagcgga tcccgcctgc cggcagcatc atgcgctggt
     3361 tgcgcaggcg tatcagtttg gcactgtcgc aatcgcggac gtccgcgaga tattccccgg
     3421 catcccgcgt ttttgcgttg acggcatcga ctgaaaggct gaagtgcgcc tcgacggcca
     3481 tggtgctcat cgcgggatcg atcgttattc gatagcgatg agggtactgc tggggcgggg
     3541 ccgcgctggc gaaaccggtg acgcaaagcg tcagcagcat cgcgcgggcg aaatacaggc
     3601 aactggaggg catgcatggg attatgcgca aggctcgcga ggatggcatc gttcaggctg
     3661 cggggtgtga ctatcctcac attcggctga aaaaaaggtg cgccagtgca tgtttgccga
     3721 aacggccttc cgcgggcacc cgggcgcagg ggcgtttagc tgccgttcag ccatttccgg
     3781 tagaatcgcg ggtcaggtaa agtagcgaaa aacggccgac gcgcccactg gtaaggagat
     3841 tcccttgagc aagatcgcga gaaggcagtt catacagtta tcggccgtag cggccgccgg
     3901 ctgtttcatt cgtcccggta cagaggcgcg agcgcaggaa ctcgtcaaga tatccgaaga
     3961 cgacccggtc gcgcagtcgc tgaagtacac gcatgatgcg agcaccgtcg acgcggcgac
     4021 ccggcccaat ccggccgaaa accaggtttg cgccaactgc gcgctgatcc agggcgaaga
     4081 gggcgagtgg cgtccctgcc agattttccc cggcaagctg atcaacgaga atggctggtg
     4141 ctccgtgtgg gcgcctaaag cctgagccgc accacaccgg tcgggttccg gcgcacctgt
     4201 gagttcgtgg tttcttccct agtcgggtcg ggcgtg
//
LOCUS       DASUOM010000279         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1208750,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000279 DASUOM010000000
VERSION     DASUOM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208750"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFY27_05890"
     CDS             <1..516
                     /locus_tag="VFY27_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5765080.1"
                     /translation="PSTEIGPLVHPRHFEKVTSYFEVAEAGGACIAAGGAVDSKIGGN
                     YVQPTLFTAATNDMRIAQEEIFGPVLTAIPFEDEAEALRLANDVAYGLAAYVWTSDIG
                     RGHRMARNLDAGMIWINSENNRHLPTPFGGMKASGIGRDGGDYSFDFYMETKNVCVAL
                     GTHRIPVLGRA"
     gene            513..848
                     /locus_tag="VFY27_05895"
     CDS             513..848
                     /locus_tag="VFY27_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765081.1"
                     /translation="MSLYQVQKFLYTLNRDPGVQQQCRDNAEQLLDNYDLTAEERTAL
                     ANGDIGLLYVLGVNGQILMHYAAFLGIEWFDYLERMREGVRRHGPVRAGVYAMTGGGA
                     SFTEEERWR"
     gene            845..1669
                     /locus_tag="VFY27_05900"
     CDS             845..1669
                     /locus_tag="VFY27_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765082.1"
                     /translation="MSLVYAGVCSHAPGITGRAELVQNKELRDEFYAALDEERRQIEA
                     AQPAAIIVIAAEHFANFFMNNMPAYCMGIGAKHQGPIEDPDWLRIERREVPGAPELAH
                     ALVKEIMQTIDVAYAEEWKFDHGIMVPLHFLTPRYDLPVIPVNINCQGPPLTPLHRAW
                     AFGEALRLACDLLPERIALVGTGGISHWPATPDSGKINEAWDRAFLDRLMRQDKQALL
                     GYDDADTYREAGQGGFEIRTYIAAAAAAGGPGTLRFYTAELPIFAVGCTVGRFELH"
     gene            1683..>2231
                     /locus_tag="VFY27_05905"
     CDS             1683..>2231
                     /locus_tag="VFY27_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5765083.1"
                     /translation="MRPAGRGELDSLYFRYPKFEFVRPPEMDNEAPRHQVIVVGAGPV
                     GLAAAIELARRGIRCVLLDAKNTLNNGSRAICVSRNSFETLQQLGVAARFESKALGWT
                     RGRCYYKDRLIYRLQMPHSADERYLPMYNIQQQYIEQFLVERAADFPDLIDLRWQNEV
                     TAVTAGGDEIVAAVETPGGAYRL"
BASE COUNT          455 a          634 c          735 g          407 t
ORIGIN      
        1 ccgtcgacgg aaattggacc gctggtgcac ccccggcatt tcgagaaggt gacgagttac
       61 ttcgaggtcg cggaagccgg gggcgcatgc atcgcggcgg gaggtgcagt cgacagcaaa
      121 atcggcggca actacgtgca gccgacgctg tttacggctg caacaaacga tatgagaatc
      181 gcgcaggaag aaattttcgg gccggtgctc acggcgattc ctttcgagga cgaagcggaa
      241 gcgttgcggc tcgcgaacga cgtcgcgtat gggctggccg cctacgtctg gacgtccgat
      301 attggacggg gccatcgtat ggccaggaac ctcgacgcgg gtatgatctg gatcaactca
      361 gaaaacaacc ggcacctgcc gacaccgttc ggcgggatga aagcgagcgg aatcggacgc
      421 gatggcggag actacagctt cgacttttac atggaaacga agaatgtgtg cgtggcgctc
      481 ggcactcacc gaataccggt cctcggcagg gcatgagtct ctaccaggtc cagaagttcc
      541 tgtacacgct gaatcgtgat cccggcgtgc agcagcaatg tcgggacaat gcggagcagc
      601 tgctggacaa ctacgatctc acggccgagg agcgtacggc gcttgcgaat ggcgacatcg
      661 gcctacttta cgtccttggg gtgaatgggc agatactcat gcattacgcg gcgtttctcg
      721 gtatcgagtg gtttgattat ctcgagcgga tgcgcgaagg cgtgcgtcgc catgggccgg
      781 tacgcgcggg cgtatacgcg atgaccggtg ggggcgcgag ctttaccgaa gaggagcgct
      841 ggcgatgagt ttggtgtatg ccggcgtctg cagccatgcc cccggtatta ccgggcgcgc
      901 ggagctggtt cagaacaaag agctgcgcga tgaattttat gctgcgctcg atgaggaacg
      961 ccggcagatc gaggcggcgc agccggccgc gatcatcgtc atcgcggcag aacatttcgc
     1021 caactttttc atgaacaata tgcctgcgta ttgcatgggt atcggcgcga agcaccaggg
     1081 gccaatcgaa gatccggact ggttgagaat cgaacgccgt gaagtgccgg gagcgccgga
     1141 actcgcacac gcgctcgtca aggagatcat gcagacaatc gatgtcgcgt acgcggaaga
     1201 atggaagttt gaccacggca tcatggtgcc gctccatttc ctgacaccgc ggtacgatct
     1261 gccggtcatc ccggtaaata taaactgcca ggggccgccg ctcacgcctt tacaccgtgc
     1321 ctgggccttc ggcgaggcgt tgcgactcgc ctgcgacttg ttgccggagc gcatcgcact
     1381 ggttggcact ggtggcatct cgcactggcc ggccactccg gattccggga agatcaacga
     1441 agcctgggat cgagcgtttc ttgatcgcct gatgcgccag gacaagcagg cgttgcttgg
     1501 atatgacgac gccgatacgt accgtgaagc agggcagggg ggcttcgaga tacgaaccta
     1561 catagctgcc gccgcggccg cgggaggccc gggtacgctc aggttctata ccgccgaact
     1621 gccgatcttt gcggtgggtt gcaccgtcgg gcgcttcgaa ctgcactgac tgggcgcacc
     1681 cgatgaggcc agcgggcagg ggcgagctcg attcactcta cttcaggtat ccgaagttcg
     1741 agttcgtgcg tccgccggag atggataacg aagcgccacg ccaccaggta atcgtggtag
     1801 gggcggggcc ggtcggactt gccgcggcca tcgaactcgc ccgccgcggc atccgatgcg
     1861 tgctgctgga cgcaaaaaac acattgaaca acggtagccg cgcaatctgc gtctccagaa
     1921 acagcttcga gacgctccag cagctgggcg tggccgcgcg cttcgagagc aaggcattgg
     1981 gctggacccg gggccgctgc tattacaagg acaggctgat ttatcgcctg caaatgccgc
     2041 attccgccga tgagcgctac ctgccgatgt acaacatcca gcagcagtac atcgagcagt
     2101 tcctggtcga gcgggctgcg gacttccccg acctgatcga tctccgctgg cagaacgaag
     2161 tgaccgccgt gaccgccggg ggggacgaaa tcgtcgcggc ggtcgagacg cccgggggag
     2221 cgtaccgttt a
//
LOCUS       DASUOM010000280         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1464868,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000280 DASUOM010000000
VERSION     DASUOM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464868"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2243)
                     /gene="ileS"
                     /locus_tag="VFY27_05910"
     CDS             complement(<1..2243)
                     /gene="ileS"
                     /locus_tag="VFY27_05910"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5765084.1"
                     /translation="MGKKTGSGAGFREVSGRYNGPELEREVLAFWREHGVFAKTLDAS
                     RERPRFSFNEGPPTANGKPGIHHVLARTFKDAFPRYKTMRGYYAPRKAGWDTHGLPVE
                     HEVEKELGIFDKKEIEEKVGIAEFTRRCRESVMRYIGDWEKMTERMGFWVNLDEAYYT
                     LDNSYIESVWHLLKVIWDKGLIYRGYKVVPYDPRIGATLSSHELALGYREVEDPSIYV
                     RFPLVDERGTSFLVWTTTPWTLPANMSLVVNPEVDYVYVELAGETLILAEALLESVLE
                     GHPHRITRVVKGEQLLGLRYVRPFDFLAVDHESAFRVLPGSFVSTGDGTGIVHAAPAY
                     GVDDLALGQSYGLPVVHAVGEDGHFLPEVTPVAGMFFKDADKPLIRLLKERGLLFRAE
                     TARHNYPFGWRTGDPIIYYAKNAWYIRTSDYRDRMVELNKTITWVPETIRDGRFGNWL
                     ENNIDWALSRERFWGTPFPVWVDDEQNYLCVGSVRELEQLCGRKLDDLDLHRPAVDDI
                     SFEKDGKTWRRVPEVIDCWFDSGAMSYAQWHYPFENREEFAEHFPADYICEAIDQTRG
                     WFYTLHAIATLVSDDVAYRNCICLSHIVDKDGKKMSKSVGNIVDPYDVFDSVGADALR
                     WYFMARVSPDIQKRISVEIVADVASSFINTFWNTYSFFVLYARLDSIDFRISPPPQER
                     PEIDRWALALLNKTIRTCTNAMDAYDAKAAGEAIETFVNQLSNWYVRRNRRRFWKSTD
                     PEDKQAA"
     gene            2402..2557
                     /locus_tag="VFY27_05915"
     CDS             2402..2557
                     /locus_tag="VFY27_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765085.1"
                     /translation="MANRPDYCADFRGSSGDSGVIFGRRRSGTFTVSRSLQERLSCLL
                     VPISVEQ"
     gene            complement(2685..3407)
                     /locus_tag="VFY27_05920"
     CDS             complement(2685..3407)
                     /locus_tag="VFY27_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX5765086.1"
                     /translation="MINPNVARISGKDDAASPAVLQVDIIADLVCPWCYLGKRRLDDA
                     LLAVHGPSSVNWYPFQLNPSMPETGMGFNEYLASRFGDPEALQPALGQLAKAGAAEGI
                     NFRFDRISHVPNTLNAHRVMKLAEAVGGNPSGLAENILRGFFEEGLDISSREVLLDLG
                     SRSGLTEASINRTLDDETTRQAVLSQEAQVRRSGVTGVPDFLINKRLFVIGAQSTASL
                     VNVFDRAMFGEESDLPVSATIH"
     gene            3666..>4255
                     /gene="msrP"
                     /locus_tag="VFY27_05925"
     CDS             3666..>4255
                     /gene="msrP"
                     /locus_tag="VFY27_05925"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX5765087.1"
                     /translation="MLIRKPPHIPSSEITSERNYLNRRQFLRDGTIAAGTFLAAPGFG
                     AVLPAATQAELAEVVRSQYSTDEEPNSFSDISTYNNYYEFGTGKEDPSRNSGEFEPRP
                     WTLTVSGHAEKTGTFDLEDFVKPYRLEERIYRMRCVEAWSMVIPWVGIPLAKVIEQFQ
                     PTSRAKFVAFETLHDPERMPGQRLRVLRWPYREGLT"
BASE COUNT          827 a         1331 c         1217 g          880 t
ORIGIN      
        1 tacgctgcct gtttgtcttc agggtcggtc gatttccaga aacgccggcg attccgccgc
       61 acataccagt tgctgagctg gttgacgaac gtctcgattg cttcgccagc ggctttggca
      121 tcgtaggcat ccatcgcgtt cgtacaggtc ctgatcgtct tgttcagcag ggccagcgcc
      181 cagcggtcga tttccggcct ttcctgtggc ggcgggctga tacggaagtc gatgctgtcg
      241 agcctcgcgt acagcacgaa gaagctgtac gtgttccaga acgtgttgat gaaggaactc
      301 gcaacgtccg cgacgatctc gaccgagatg cgcttctgga tatcaggcga tacgcgcgcc
      361 atgaagtacc agcgcaaggc atcggcgccg accgaatcga atacgtcgta agggtcgacg
      421 atgttgccga ccgacttcga cattttcttg ccgtccttgt cgacgatgtg gctcaggcaa
      481 atgcagtttc tgtacgcaac gtcatcggac accagggtcg ctatcgcatg gagcgtataa
      541 aaccagccac gggtctggtc aatcgcctcg cagatgtagt ccgccgggaa atgctccgcg
      601 aactcctccc ggttctcgaa cgggtaatgc cactgcgcgt aggacatcgc gcccgagtcg
      661 aaccagcagt cgatgacctc agggacgcgc cgccacgtct tcccgtcctt ctcgaaactg
      721 atgtcgtcca cggcgggacg atgcaggtcc aggtcgtcga gctttcgtcc gcacaattgc
      781 tccagttcgc ggacggagcc cacgcacagg taattttgct cgtcatcgac ccacacaggg
      841 aacggtgttc cccagaagcg ctcccgcgac agcgcccaat cgatgttgtt ctccagccag
      901 ttgccgaacc tgccgtcgcg aatcgtttcc ggcacccacg ttatggtctt gttgagctcg
      961 accatccggt ccctgtaatc cgaggtgcga atgtaccagg cgttttttgc gtaatagatg
     1021 atcgggtcgc cggtgcgcca gccgaacgga taattgtgcc gggccgtctc cgcgcggaac
     1081 aggagccctc gctccttcag gaggcggatc agcggcttgt cggcatcctt gaagaacatt
     1141 cccgcgaccg gggttacttc cggcaggaag tgaccatcct cgccgactgc atgcacgacc
     1201 ggcagcccgt aggactgtcc cagagcgagg tcgtccacac cataggccgg tgcggcatgt
     1261 acgatccccg taccatctcc cgtgctgaca aagctgccgg gcaggacgcg aaaggcgctc
     1321 tcgtggtcca ccgccaggaa atcgaacggc cgtacatatc gcaggccgag caactgttcc
     1381 cctttcacca ccctcgtaat cctgtgcggg tgcccctcga gcaccgactc cagcagcgct
     1441 tccgccagga tcagcgtttc gccagcgagt tccacatata cgtaatccac ttccggattg
     1501 acgaccagcg acatgttggc cggcaatgtc cagggcgtcg ttgtccagac gaggaaggaa
     1561 gttccccgct cgtccacgag cgggaaacga acatagatcg aaggatcttc gacttcgcga
     1621 tacccgagcg cgagttcgtg cgatgaaagc gttgctccga tacgcgggtc atacggcaca
     1681 accttgtagc cgcgatatat gagtcccttg tcccagatga ccttcaacaa atgccagacg
     1741 ctttcgatgt agctgttgtc cagcgtgtaa taggcttcgt cgaggtttac ccagaagccc
     1801 atgcgttctg tcatcttctc ccagtcgccg atgtagcgca tgacggactc ccggcagcgc
     1861 cgcgtgaact cggcaatccc caccttttcc tcgatttcct ttttgtcgaa gatgccgagt
     1921 tccttttcga cctcatgctc gacaggcagg ccgtgagtgt cccagccggc ctttcgcggc
     1981 gcgtagtagc cgcgcatcgt cttgtaacgg gggaacgcgt ccttgaaggt gcgcgcaagg
     2041 acgtgatgga ttccgggctt accgttcgca gtgggcgggc cttcattgaa gctgaagcgg
     2101 ggccgttcgc gcgaggcatc cagggtcttt gcgaagacgc cgtgctcacg ccagaaagca
     2161 aggacttccc gttccagctc cggtccgttg tagcggccgg aaacttccct gaaacctgcg
     2221 ccagatcctg ttttcttacc catcctggac cattccgttc caataccgcc agcgccccaa
     2281 agcaaaacgc cccggacgca tcccattgcg tccggggcga cagaatccaa ctcgatcgcg
     2341 gtaccacccg gtttcacaac ggcccgaacc ttaaagccgc tgcctcataa agcctttgac
     2401 ggtggccaac cggccggact actgcgcgga tttccgcggt tcgagcggcg actccggggt
     2461 gatattcggc aggcggcgat ccgggacttt caccgtctcc cggtcgctgc aggaacgcct
     2521 ctcctgccta ctcgtcccca tcagcgtcga gcagtgaaag ctatcgttcg caggcgaaaa
     2581 ttacaagcac cctcgagggg agcctctgca caagtcatgg tcggcatctt tccggccagg
     2641 acccaaagct ctgcgttgtg cagaggctcc ctggtgaatg ctccctagtg gatggtcgcg
     2701 gacaccggca ggtcgctttc ctcgccgaac atcgcgcgat caaagacgtt gacgagactg
     2761 gcggtgctct gggcgccgat cacgaaaagc ctcttgttga tcaggaagtc cggcacgccc
     2821 gtaacgccgc ttcgccgaac ctgtgcttcc tgcgacagta ccgcctggcg cgtcgtttcg
     2881 tcgtccaggg tcctgttgat gctggcttcc gtcaaaccgc tcctgcttcc caggtcgagc
     2941 aagacttccc tgctggagat atccagtcct tcctcgaaga agccgcgcag gatattttcg
     3001 gcaaggcccg atgggttccc accaacggct tctgccagtt tcataacgcg atgcgcattg
     3061 agcgtgttgg gcacatggct gatccggtcg aaacggaagt tgatcccctc ggccgctccc
     3121 gctttcgcca gctgtccgag tgccggctgc agcgcttcag gatctccgaa tcgcgaggcc
     3181 aggtattcgt tgaagcccat gcctgtctcg ggcatcgagg ggttcagctg gaagggatac
     3241 cagttgacgc tgcttggtcc atgcactgcc agcagcgcat cgtcgagcct tcgcttgccg
     3301 aggtaacacc atgggcaaac caggtcggca atgatgtcca cctgcagcac cgccggcgac
     3361 gccgcatcgt ccttgcctga aatccttgct acatttggat tgatcattgg agcttcccgg
     3421 aaatcctctg gtccgatgtc attgagaccc ccctgagatg tggccggttc accgcaaatc
     3481 aagattgtcc ggctggcctc gaggtagaat ttatgcgggt cttttcgctg catttaggcg
     3541 catattccat tgtgttggcg ccatttcagg cggcatgcct gcctccaccc gccggctctt
     3601 tagtgaaata aaacggggct ggttccggtc ttggtaaacc tgaggacatc aacgggagaa
     3661 gcacgttgct cattcgaaag ccacctcaca tcccctcgtc ggagatcacc agcgagcgaa
     3721 actacctgaa ccgccgccag ttcctgcggg acgggacgat cgcggccgga acatttctcg
     3781 ccgcgccggg gttcggcgcc gtcctgcctg ccgcaacgca ggccgagctt gccgaggtcg
     3841 ttaggagcca gtacagcacc gatgaggagc cgaacagctt ctctgacatc agcacctaca
     3901 acaattacta cgagttcggc accggcaagg aggacccgtc gcgcaattcc ggcgaattcg
     3961 agccacggcc atggaccctg accgtatcgg ggcacgccga gaaaaccggt accttcgacc
     4021 tggaggattt cgtcaagccg taccggctcg aagaacgtat ctacaggatg cggtgtgtcg
     4081 aggcctggtc gatggtcatc ccgtgggttg gcatcccgct ggcaaaggtc atagaacaat
     4141 tccagccgac gtccagggcg aagttcgtgg cattcgagac cctgcacgac cccgagagga
     4201 tgccgggaca aaggttacgg gtgcttcgct ggccctaccg ggaagggctg acgat
//
LOCUS       DASUOM010000281         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_367212,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000281 DASUOM010000000
VERSION     DASUOM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367212"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="lptD"
                     /locus_tag="VFY27_05930"
     CDS             complement(<1..1202)
                     /gene="lptD"
                     /locus_tag="VFY27_05930"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX5765088.1"
                     /translation="MSRHSLSFASLALLLALSPARGQQSLICEAPSTRQLAEEAAAAG
                     LDADSDTFEFETGNVEAGVGDDPTMRMTGGVLVRRGNRLAGAENAIYNPETRSLLLDG
                     GVRYEDPESQVSSESAELAYGSGRIRFEGAEFQLGQNNSRGAADVLQISEDGTVSLDE
                     VRYTTCPPESEDWLLQAGDIDLDTREGTGTARGVKLRFKGVPILYSPWLSFPITDARK
                     SGILTPELGSAGRSGNELRIPWYWNIADNYDATLTPRFLSDRGLQVGSEFRYLTQRMD
                     GIAQFEYLADDNLANRNRHLINFNHRALFDNGWRNLIDFRQASDSNYFEDLGGSLSTS
                     SITHLNRSVMFDFYGEHWSLFGRLQDYQTIDSAILPEDEPYRRLPQLGASGYWPDRAL
                     GMRYRFDG"
     gene            1330..2391
                     /locus_tag="VFY27_05935"
     CDS             1330..2391
                     /locus_tag="VFY27_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5765089.1"
                     /translation="MTREQKIDKSSTQEDRRLDQLREWLACFDDIEPATLAPASIDAS
                     FRRYFRVRAGSESLIVMDAPPEREDCRPYIRVARYLEQMSLNSPRIVRAAPEQGFLLL
                     TDLGCRQFLQELEKRPERAGELYGDALSALLKLQEQGRRFQSDLPSYDDKLLGFEMSL
                     FRDWLCLRHLGLELSERDEAEWQSSVELLVRNALNQPKVFVHRDFHSRNLMVTDRNNP
                     GILDFQDAVEGPFTYDLVSLLKDCYIRWPAEQVKRWATQFHSALAERDLVSISVAQFM
                     RSFELMGVQRQLKAAGIFARLNHRDGKPAYLQDVPRTLGYIVEIAPRYPELEFLEQLI
                     RERCLPALAAARPAGEVRL"
     gene            2400..3074
                     /locus_tag="VFY27_05940"
     CDS             2400..3074
                     /locus_tag="VFY27_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5765090.1"
                     /translation="MILAAGRGERLRPLTDKVPKALVEVGGVSLLERQLERLARAGVR
                     TAVINLGWLGEAIVQRIGDGGRFGLQVVYSPEYDDVLETGGGIRRALPLLGRDPFWVL
                     NSDVFTDISLPPAELAPGCSGHLVLVPVPPHRPRGDFDLVDGKVRKSEQPALTFSGMA
                     IYRPELFGNEEVRRFPLAPLLFDAAERGELSGELYEGVWEDVGTIERLERLNARETLH
                     KPEGAL"
     gene            complement(3081..3725)
                     /gene="lipB"
                     /locus_tag="VFY27_05945"
     CDS             complement(3081..3725)
                     /gene="lipB"
                     /locus_tag="VFY27_05945"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5765091.1"
                     /translation="MTARASPLPQHAAIEVRSLGRREFEPVWRDMQRFTNERTGDTRD
                     EIWFTEHPPVFTLGLNASREHLLAPGDIPVVQIDRGGQVTYHGPGQLMVYPLLDLKRA
                     GLNIRDLVSALERSVIGLAGEFGIEASARRDAPGVYVEDRKLASVGLRVRRGASYHGM
                     ALNVDLSLEPFSRINPCGYQGLQMIDLARLGVTEGMQAISSRLEPHLLRNFGLM"
     gene            complement(3722..3982)
                     /locus_tag="VFY27_05950"
     CDS             complement(3722..3982)
                     /locus_tag="VFY27_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX5765092.1"
                     /translation="MSDDVMEFPCEFPIKMMGRDQPEFREAAIAVVERHAGHVEEDAV
                     RIAASSNGNFLSITVTITATSREQLDNIYRDLTDDEQILVAL"
BASE COUNT          735 a         1229 c         1286 g          770 t
ORIGIN      
        1 tcaccgtcga agcggtaacg catgccaagc gcgcggtcgg gccagtagcc actcgcgccg
       61 agctgcggaa ggcgccggta gggttcatcc tccggaagga tagcgctgtc gatcgtctgg
      121 taatcctgca ggcggccaaa cagcgaccag tgttcgccgt agaaatcgaa catcacgctg
      181 cggttcaggt gcgtgatgct cgaggtactc aggctgccgc ccaggtcttc gaaatagttg
      241 ctgtcggacg cttgccggaa gtcgatcagg ttgcgccagc cgttatcgaa cagtgcgcgg
      301 tggttgaagt tgatcaggtg ccggttccgg ttagccaggt tgtcgtccgc caggtactcg
      361 aactgcgcga tgccgtccat gcgctgcgtg agatagcgaa actcggagcc cacctgcagg
      421 ccgcggtcgg acaggaagcg cggagtgagc gtcgcatcgt aattatcggc gatgttccag
      481 taccagggga tgcgaagctc gttaccgctc ctccctgcgc tgccgagttc gggtgtaagg
      541 atgccggact tcctcgcatc ggtaatcggg aaggacaacc atggcgaata cagaatcggc
      601 acgcccttga aacgcagctt gacgcccctg gcggtgcccg tgccttcgcg cgtgtcgagg
      661 tcgatgtcgc cggcctgcag caaccagtct tcggattccg gagggcaggt cgtgtagcgc
      721 acctcgtcca ggcttacggt gccgtcttcg ctgatctgca gcacgtccgc ggcaccgcgc
      781 gagttgttct gcccgagttg gaactccgca ccctcgaagc ggatccgacc cgagccatac
      841 gccagttccg cagactcgct ggaaacctgg gactcgggat cttcgtagcg cacgccgcca
      901 tcgagcagga gggagcgggt ttccgggttg tagatcgcgt tctccgcacc ggccagccgg
      961 ttgccacggc gcaccagtac cccgccggtc attctcatcg tagggtcgtc gccgaccccg
     1021 gcctcgacgt tgccggtttc gaactcgaaa gtgtcggagt cggcatcgag accggctgcg
     1081 gccgcttcct ctgcaagttg ccgggtcgac ggggcttcac aaatcagtga ctgctgtccc
     1141 cgggcaggcg acagcgccag caggagtgcg aggctggcga atgagagtga gtgtctggac
     1201 aaaattgcgg ctggagttcg taaacggcag tcaaccctgc gggacgcagg aaaagatgtt
     1261 atcaggtatt gcgatttgct accttcgcgc agaatgaagg cccggagttt tcacaaagtg
     1321 ctccgggcta tgacccggga gcagaagata gacaaatcat cgacgcagga ggaccgtcgc
     1381 ctcgaccagt tgcgagagtg gcttgcatgc ttcgatgata tcgagcccgc aaccctggct
     1441 ccggcttcca tcgacgcaag cttccgtcgt tacttcaggg tacgggcggg aagtgaaagc
     1501 ctgatcgtaa tggacgcgcc gccggaacgt gaagactgcc ggccttacat tcgcgtcgcc
     1561 cggtatctcg aacagatgag cctgaacagt ccgaggatcg tgcgcgcagc tcctgaacag
     1621 ggcttcctgc tgctgacgga cctggggtgc cgccagttcc tgcaggaact tgaaaagcgg
     1681 ccggagcgcg cgggcgaatt gtatggcgat gccctttcag cactgctgaa gttgcaggag
     1741 cagggccgcc gcttccaaag cgatttgcct tcctatgatg acaagctcct gggcttcgag
     1801 atgtctctgt ttcgcgactg gctgtgcctg cggcatctcg gcctcgagtt gtcggaacgc
     1861 gatgaggccg aatggcagtc atctgtggag ctgctggtgc ggaatgcgct gaaccagcct
     1921 aaggtattcg tccatcgcga cttccattcg cgcaacctga tggtcaccga caggaacaat
     1981 cccggcatcc tggacttcca ggatgcggtc gaaggcccgt tcacttacga cctggtgtcg
     2041 ttattgaagg actgctacat ccgctggccg gcagagcagg tgaaacgctg ggcgacgcag
     2101 tttcactcgg ccctggcgga gcgcgatctg gtgtcgataa gcgtcgcgca gttcatgagg
     2161 agctttgaac tcatgggtgt ccagcgccag ctcaaggcag cgggaatttt cgcaagactc
     2221 aatcacaggg atggcaagcc ggcgtacctg caagatgttc cgcgcacgct cgggtacatt
     2281 gtcgagatcg cgccgcgcta cccggagctg gaatttctcg aacagctgat ccgcgagcgc
     2341 tgccttccgg cgttggctgc ggcgcgcccg gccggcgaag tgcgcctttg acgatcgcta
     2401 tgattttagc cgcggggcgc ggcgagcggc tgcgaccgct tacagacaag gtgccgaagg
     2461 cgctcgtcga ggtcggcggc gtgagcctgc tcgagcgaca gctcgagcgc ctggccaggg
     2521 ccggcgtccg gactgcggtg atcaacctgg gctggttggg cgaggcgatc gtacagcgca
     2581 taggtgacgg cggccgcttc ggcctgcagg ttgtgtacag ccccgaatac gatgatgtcc
     2641 tggaaacggg cggcggaatt cgccgcgcgc ttccgctcct cgggcgcgac ccgttctggg
     2701 tgctgaactc cgacgtcttc accgatatct cgttgccgcc cgccgaactc gctccgggct
     2761 gctcgggtca cctcgtatta gtgccggtac ctccgcacag gccacgcggc gacttcgatc
     2821 tcgtcgatgg caaggtgcga aaatccgaac aacctgccct cactttcagc ggcatggcga
     2881 tctaccggcc tgaactgttc gggaacgagg aggtgcggcg cttcccgctc gcgccgttgc
     2941 tgttcgacgc cgcggaacga ggagagctct ccggggagtt atatgaaggt gtgtgggaag
     3001 acgtcggcac catcgagcgg ctcgagcggc tcaatgccag ggaaactctg cacaagccgg
     3061 aaggagcgct gtaggggctc ctacatcagc ccgaagtttc gcagcaggtg gggttcaagc
     3121 cgtgaactga tcgcctgcat gccttccgtc accccgagac gtgcaagatc gatcatctgg
     3181 agtccctggt agccgcaggg attgatgcgg gaaaagggtt ccaggctcaa gtccacgttg
     3241 agcgccatgc cgtgatagct ggcgccgcgc cgcacgcgca agccaacgct cgcgagcttc
     3301 ctgtcctcga cgtatacccc cggcgcgtcc cgtcgcgcgg aagcctcgat gccgaactcc
     3361 cccgcgaggc cgattaccga gcgctccagc gcggacacga gatctcgaat gttgagtccg
     3421 gcgcgcttca ggtccagcaa cgggtacacc atgagctgac ccggtccgtg atacgtcacc
     3481 tggccaccgc gatcgatctg cacgaccggg atatctcctg gcgccagcag atgttcgcgg
     3541 cttgcgttga gtcccagcgt gaacaccggc gggtgttcgg tgaaccagat ctcgtcgcgg
     3601 gtatcgccgg tccgttcgtt cgtgaaccgc tgcatgtccc tccacacagg ctcgaactca
     3661 cgccgcccca gcgaccgcac ctcgatcgca gcgtgctgcg gcaggggaga tgccctcgct
     3721 gtcacagcgc caccaggatc tgttcgtcgt ccgtcaggtc gcgatagata ttgtccagct
     3781 gctcgcggct ggttgcagtg atggtcacgg tgatcgacag gaagttgcca ttgctgctcg
     3841 ccgcgatgcg aacggcatcc tcttccacgt gacctgcatg ccgctccacc acggcgatgg
     3901 cggcctcgcg aaactcgggc tggtcgcgcc ccatcatctt gatggggaac tcgcacggaa
     3961 actccattac atcgtctgac atcggctcga accacaggtg caggcattat tcatgaacgg
//
LOCUS       DASUOM010000282         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_930099,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000282 DASUOM010000000
VERSION     DASUOM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930099"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFY27_05955"
     CDS             <1..1101
                     /locus_tag="VFY27_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5765093.1"
                     /translation="AFNIFVFMEISSLATYILVAGGPKRQALSAVFKYLIMGTIGATF
                     YLIGVGLIYMLTGTLNLADMEARIHEVSDLNPILVAGGFITIGLALKAAVFPLHGWLL
                     GAYTYAPHAVTVFVAACSTKVFLYILLRFDFFVFQGNLPGHIAQFSGFILPLAIAAAL
                     VGSGIALFERDLKRLLAWSSVAQIGYIMFGASLVSATGLTAGIAHMFNHALAKGALFL
                     AIASLATTYPTLTIDGIAGAAKRMPFTMAAFVVAGLSLIGIPGTAGFVSKWYLVLAAV
                     EGGVPGALLVVPILASSVMAVLYIWKIVESAYFTARAPNPEVHGNGRSEAPWWVLAVL
                     WGVVLLNVYFGLAPALPLELATKAAAALLART"
     gene            complement(1127..1273)
                     /locus_tag="VFY27_05960"
     CDS             complement(1127..1273)
                     /locus_tag="VFY27_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765094.1"
                     /translation="MLAAAPVVTAGLEWWWLDERFVRSQLVGILMVIAGATTLSQQRA
                     TGDL"
     gene            complement(1249..2004)
                     /locus_tag="VFY27_05965"
     CDS             complement(1249..2004)
                     /locus_tag="VFY27_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5765095.1"
                     /translation="MTLPSHIAPLVAIASLGTVHFVEKLAIHQGAPPGPFAFGQTAVA
                     MLVLAPVWVWRLRKDDRKSLRHLPLASLALIGILASGVVVLLSIMALSYTSATHKGVI
                     QASYPLGTMFFAWVLLRERITPAGYVAAAVIVAGLVLMTSRGLGTAPNLGDWILVGTV
                     PLMGFSDAFSKKVLKDVSPLTVSFGRYFFGTAFLAVLLSVLDLGQLGDLLPVWQLLLA
                     AGMMTALAMELFYCRSIASDRRSRPACWPLRLW"
     gene            2165..2662
                     /locus_tag="VFY27_05970"
     CDS             2165..2662
                     /locus_tag="VFY27_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765096.1"
                     /translation="MHVETWSNNEIRQSWQETLQDALLAGSIVCVVMLATVAIRGRAD
                     SGSAVAPINATSHVVYGPEAANVDMPDAKHTLPGLAINAGASVFWATLYERLFGRGNS
                     GHVGRTLLGGAVVAALAYLVDYHLIPKRLTPGWEERVSGRSLGYIFGAMALSLPVRSV
                     IKRLL"
     gene            2757..3494
                     /locus_tag="VFY27_05975"
     CDS             2757..3494
                     /locus_tag="VFY27_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5765097.1"
                     /translation="MRILFAVSPLLFAILIPNLSQADWPQFPFVHAEGEARVEVPPEK
                     ASIRVSLTTFHRESEIGLETLQVQLTKAVDVLEQAGVPEDQITGFDLKKMVVRSRDDD
                     NNELEILGYYFSRDFEVELTDLETFSALVTELMAIDNVTTVDADFDIENRDRVESNLV
                     REAGEDARRRAEEMARTMDVSIESVFAISESPVASVGLAFGLTSDSYTVMGERPSKRG
                     LFTSTIFVPEAIELRKSVNVLFRLRER"
     gene            3618..4433
                     /locus_tag="VFY27_05980"
     CDS             3618..4433
                     /locus_tag="VFY27_05980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5765098.1"
                     /translation="MSAGEQADFHCRLCAGDQLELYYRLGAEGQFRYYRCANCHLVNL
                     DLKAGLDQTQYTDEWTDPTDDNAKRNRDNDATYEFIRRRIATPGRLLDLGCGHGRLLY
                     RARKDGWDVKGVELSTETAARVRAEIGVPVVAGNFLQITPSEADLSGFDLICLRHVLE
                     HLPDSLLAMNKIRELLAPGGRVLLEMPNIEGIDTKLKRWMADRGLHRKNFASDYVVGH
                     CNEFCRESFAYLAAKTGFRLLRWETYSKKRLTNLIYNRIPIGNKARVLITRSD"
     gene            complement(4534..>4841)
                     /locus_tag="VFY27_05985"
     CDS             complement(4534..>4841)
                     /locus_tag="VFY27_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765099.1"
                     /translation="FSDTWALTLEGYGTVDRLGSSGHPSGSAELFGDFDQHRAGPVLY
                     YSVGLGGSRDAEQLRSRSALAPGGEEGPTLTVGLGLLEGLNRDTPDHTLKLSIEVDF"
BASE COUNT         1017 a         1438 c         1424 g          962 t
ORIGIN      
        1 gcgttcaaca tcttcgtgtt catggagatt tcttcgcttg cgacgtacat cctcgtcgcg
       61 ggcggcccga agcgacaggc gctttcagcg gtgttcaagt acctgatcat gggcacgatc
      121 ggcgctacgt tctacctgat cggtgtcgga ctgatctata tgctgaccgg aacgctgaac
      181 ctagccgaca tggaagcgcg tatccatgag gtatcggacc tgaacccgat actcgttgcg
      241 ggcggattca tcaccatcgg gctggctttg aaggccgcgg tcttcccgct gcacggttgg
      301 ctgctgggcg cctacacgta cgctccccat gctgtgaccg tgttcgtcgc cgcctgctcc
      361 acaaaggtct tcctctacat actcctacgc tttgattttt tcgttttcca gggaaacctg
      421 ccaggacaca tcgcgcagtt ctccgggttc atcctgccgc tcgcaatagc cgccgcgctg
      481 gtcggctccg gaatcgcgct gttcgagagg gatttgaagc gcctgctcgc ctggtcctcc
      541 gttgcccaga taggctacat catgttcggc gcaagcctcg tgtcggcgac aggactcacc
      601 gccggaattg cgcacatgtt caatcacgcg ttggcgaagg gggcactgtt tctcgcgatt
      661 gcctcgctcg caaccacata tccgacgctc acgatagatg gtatcgccgg tgcggcaaaa
      721 cgaatgcctt tcaccatggc cgcgttcgtc gttgccgggc tgagcctcat cggtatacca
      781 ggcaccgccg ggtttgtcag caaatggtat ctcgtactcg ctgcggtgga gggaggtgtt
      841 cccggcgcgc tgctggtcgt accgatcctc gcaagctcgg taatggcagt cctgtacatc
      901 tggaaaatag tcgaatccgc gtacttcacc gctcgagcac ccaaccccga ggtgcacggg
      961 aatggccggt cggaagcacc ctggtgggtg ctcgcggtac tttggggggt cgtactcctg
     1021 aatgtttact tcgggctcgc gcctgcatta ccgctggaac tggcgacgaa agcggccgcg
     1081 gccttgctgg cgcgaacatg aaaccgtcgg aaggttcggt cgctccctac aggtcgcccg
     1141 tcgctcgctg ttgcgacagc gtcgtagctc ccgcgatcac catcagaata ccgacgagtt
     1201 ggcttcggac gaagcgttca tcgagccacc accattcgag gcccgcggtc accacaggcg
     1261 cagcggccag catgccggcc gcgagcgacg gtccgatgcg attgatcgac agtaaaacaa
     1321 ctccattgcc agcgcggtca tcattccggc tgcaagcaac agttgccata ccgggagaag
     1381 gtcgcccaac tgaccaaggt cgagcaccga cagcagaacc gccaggaaag cggtgccgaa
     1441 aaaataacgg ccgaagctca ccgttagcgg cgagacgtct ttcaagactt tttttgagaa
     1501 ggcgtccgag aatcccatca gcggaacggt gccgaccagg atccagtcac ccaggttcgg
     1561 tgcggttccc aacccgcggc tggtcatcag caccaggccc gcaacgatca ccgccgcagc
     1621 cacgtagcca gccggcgtga ttctttcgcg taacagtacc cacgcgaaga acatggtgcc
     1681 caatggatag gaagcctgga tgacgccctt gtgcgtagcg gatgtgtaac tgagcgccat
     1741 gatactcagc aggacgacca cgccggaggc cagtatgccg atgagtgcaa gggaagccag
     1801 aggcaggtgc cgcagtgact tcctgtcgtc tttccggagc cgccaaaccc agacaggagc
     1861 gagcaccagc attgctaccg ctgtctgtcc gaacgcgaat ggcccgggag gcgccccctg
     1921 gtggatcgca agcttctcga cgaagtgcac cgttccgaga ctggcgattg cgaccagcgg
     1981 cgcaatatgc gaaggaagcg tcattaggag cgtgtcaata atcgaaagca cccttcaggc
     2041 aagttgtgtg ccagcaggaa cgctcgacct cctggtctga ctgggatcac cgtcgtcctt
     2101 tccgtcattg cctggcgccg gcacgaaagt tgcagcagta ctctccatcg aaggagacat
     2161 tgatatgcac gtagagacct ggagcaacaa tgagattcgc caatcctggc aggagacgct
     2221 gcaagatgcc ctgctggccg gctctatcgt ctgcgtggtg atgctggcca cagtggcgat
     2281 tcgcggcagg gccgacagcg gcagcgctgt tgctcccata aacgcgacga gccatgtcgt
     2341 ttacggtccg gaggcggcca acgtcgatat gccggacgcc aaacatacgc tgcctggtct
     2401 tgcaatcaac gccggcgcca gcgttttctg ggccactctt tacgagcggc tgttcggtcg
     2461 cgggaacagc ggccacgtcg gcaggacctt gctcggcgga gccgtggtgg cggcgctagc
     2521 gtatctcgtc gactaccatc tgatcccgaa gcggctgacg ccgggctggg aagaacgggt
     2581 ctctggccga tcgttgggct atatctttgg cgccatggca ttgagcctgc ctgttcggag
     2641 cgtaataaag cgactcctgt aacacttctc gaccaccgtc accggcatag ccgggggaag
     2701 gctcatgtat actccccggt acggcaactt gaacaataaa taatcgagga agatcaatga
     2761 gaatcctctt tgctgtcagc ccgcttttat tcgccattct tattccgaac ctttctcagg
     2821 cggattggcc ccagttccca ttcgttcatg cagaaggcga ggcccgtgtt gaagttcctc
     2881 ctgaaaaggc aagcatccgc gtcagcctga ccacgtttca cagagagtca gaaataggcc
     2941 tcgagactct gcaggtgcaa ttgacgaaag cggtcgacgt tctggagcag gcaggggtgc
     3001 cagaggacca gatcaccggc ttcgatctca agaaaatggt ggtgaggtca agggacgacg
     3061 ataacaacga actggaaatc ctcggatact atttctcgcg agacttcgaa gtggagctga
     3121 cggacctgga gacgttcagc gcactcgtca ccgagctgat ggctatcgac aacgtaacca
     3181 cggtcgacgc cgatttcgat atagaaaatc gtgaccgggt cgaatcgaat ctggtccggg
     3241 aggcaggcga ggatgcccgt cggagagccg aagagatggc aagaaccatg gacgtatcga
     3301 tcgagtcggt tttcgcgatc tccgaaagtc ctgtcgcgtc ggtcggactg gcattcgggc
     3361 tgacgtctga ttcctacaca gtaatgggag agagaccctc caagcggggc ctgttcacca
     3421 gcactatctt cgtgcccgaa gcgatcgagc ttagaaaatc cgtaaacgtg ttgttcaggc
     3481 tcagggagag ataaaagcag cggcgagcct gtcgtcatgg cttgatacgc tgcggtaaga
     3541 aggctcagct gatatccagg atcagaactc tcctgaactg ataccatttt cggcagtccc
     3601 tggtcaggga gagagatgtg tctgcgggag agcaggccga cttccactgt cggctttgtg
     3661 cgggagatca gctggagctt tactaccgcc ttggtgctga aggccagttt cgctattacc
     3721 gttgcgccaa ctgtcacctg gtgaacctgg acctcaaagc gggactggac cagacgcagt
     3781 acaccgacga gtggaccgac cccaccgacg acaacgcaaa gcgaaatcga gacaacgacg
     3841 cgacgtacga atttatccgc cgcagaatcg caacgcccgg gcggctcctc gacctcggct
     3901 gcggtcatgg gcgcctgctc taccgcgcca ggaaagacgg ctgggatgtc aaaggcgtag
     3961 aactgtcgac agagacggcc gctcgtgtgc gagcagagat cggtgtccct gtcgtcgctg
     4021 gaaatttcct ccagatcacg ccgtcggagg ccgatctttc cggtttcgat ctcatctgcc
     4081 tgaggcacgt gctcgagcat cttcccgact ccctgctcgc catgaacaag atccgggagc
     4141 tgctcgctcc cggcggcagg gttttactgg agatgcctaa tatcgaggga atcgacacca
     4201 agctgaagcg gtggatggct gacaggggtc ttcatcggaa aaactttgcc agcgattacg
     4261 tcgtcggaca ctgcaacgaa ttttgccggg agtcattcgc ataccttgcc gcgaagacgg
     4321 gttttcgcct gctgagatgg gagacgtatt cgaagaagcg acttaccaac cttatctaca
     4381 accgcatccc gatcgggaac aaggcgcggg tgctgataac gcgcagcgac tgaccgtcga
     4441 cctgcgcgtg catattgcgc atagccttcg ctatagccgc ctgcgcatca cgcgcggccc
     4501 cggctacgca atcctgtggg cggccattgc agatcagaaa tctacttcga tgctcagctt
     4561 cagcgtgtga tcgggcgtat cccgattaag gccttcgagc agccccagcc cgaccgtgag
     4621 cgtcggacct tcctcgccgc caggcgcgag tgccgacctt gagcgcaact gttccgcatc
     4681 ccgcgaacca cccaggccta ccgagtaata aagcaccggc cccgcccggt gctggtcaaa
     4741 atcgccgaat aactctgccg atccagaagg atgcccgctg ctgccgagcc gatctacggt
     4801 gccgtaacct tcgagcgtga gcgcccaggt gtcggaaaag g
//
LOCUS       DASUOM010000283         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_135705,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000283 DASUOM010000000
VERSION     DASUOM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135705"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFY27_05990"
     CDS             <1..966
                     /locus_tag="VFY27_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5765100.1"
                     /translation="RGYSPEVATGTICASGTLGQIIPPSIILVMLGDVMSSAFQQAQL
                     AQGDFSPRTVSVGDLFAGALIPGLLLVGLYMTYLFAVALFRPSSMPPHVASAADKIKL
                     VQILNALLPPLLLILAVLGSILGGFATPTEAAGVGAMGALGIALKRRALTLQRLRSIM
                     RSTLSVSSMVFFILIGASVFSLVFRGYGGDDAVRNLLDALPGGVFTAVLLVMLVMFLL
                     GFVLDFIEIVFVVVPIVGPILLSMGLDPVWLGIMIAVNLQTSFLTPPFGFALFYLRGV
                     APPEVTTGQIYRGVMPFVAIQLLALLILAAIPELATWLPGKLYGR"
     gene            complement(1172..2266)
                     /locus_tag="VFY27_05995"
     CDS             complement(1172..2266)
                     /locus_tag="VFY27_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5765101.1"
                     /translation="MKRRQFVGGLALAAGVTACGDKAAGPDGPEERFSESFSWNLVTS
                     WPPGLPGLGTGAINLARRIGRASGGRLNIKVYAGGELVPALEVFDAVSRGTAQMGHDA
                     SYYHRGKVPAAQYFTAIPFGQNATETNGWLYYGGGLELWRELYAPFNIVPFPTGQTGV
                     QMAGWFNKKINSVADLRGLKMRMPGIGGEVLQRAGATQVTVPASEVFTALQTGAIDAA
                     EWVGPYNDVSLGLHKVARYYYYPGWQEPGPMIECLVNKDAWESLPEDLQEIVTASCQA
                     ANMDMQAEFTYGNAMALAQLADDPNVEILQLPDDVLSLLKRLSGEIVDELSARDPWAK
                     RLQESFFGFLEKSAENQRITELAYLNTREL"
     gene            2726..3163
                     /gene="dtd"
                     /locus_tag="VFY27_06000"
     CDS             2726..3163
                     /gene="dtd"
                     /locus_tag="VFY27_06000"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5765102.1"
                     /translation="MIGLLQRVTEASVTVEGRVVASIGRGLLVLVAVTREDTEEAVSR
                     LMERILTYRVFPDDCKRMNLCLEELGLDLLLVPQFTLAADTRKGRRASFTGAAEPETG
                     ARFFDLMVKLAAERLSRVESGIFGADMQVALVNDGPVTFWLES"
BASE COUNT          532 a         1002 c          986 g          666 t
ORIGIN      
        1 cgggggtatt cgccggaggt cgccaccggc accatttgcg cttcgggcac gctcggacag
       61 attatcccgc cgtcgatcat cctcgtgatg cttggggatg taatgtcttc ggcgttccag
      121 caggcgcagc ttgcgcaagg cgatttctcg ccccggactg tctccgtcgg cgacctcttc
      181 gccggtgcgc tgataccagg cctgctgctg gtaggtctct atatgactta cctgttcgcc
      241 gttgccctgt ttcgcccgag ctcgatgccg ccgcatgtcg cctccgccgc agacaagatc
      301 aagcttgtac agatcctgaa cgcactgctg ccgcccctct tgctaatcct tgcggtgctg
      361 ggttccatac tcggcgggtt cgcgacaccg accgaagccg ctggcgtcgg cgcgatgggc
      421 gcgctcggca tcgcccttaa gcgccgcgcg ctcacgcttc agcgtctccg cagcatcatg
      481 cggagcacgc tgagcgtcag tagcatggtg tttttcattc tcatcggcgc ctccgtgttc
      541 tcgctcgtgt ttcgcggcta cggaggcgac gacgcggtgc gaaacctgct cgacgcgctt
      601 cccggcggcg tgttcaccgc cgtccttctc gtcatgctcg tgatgttcct gctcggcttc
      661 gtcctcgact tcatcgagat cgtcttcgtg gtcgtaccca tagtcgggcc gatccttctc
      721 tccatgggcc tcgacccggt ctggctcggc atcatgatcg ccgtcaacct gcaaacgtcc
      781 ttcctgacgc cccctttcgg ctttgcgctt ttctacctgc gcggcgtcgc cccgcccgaa
      841 gtcaccacgg ggcagatcta ccgcggcgtg atgccattcg tggcaataca gctgctggca
      901 ttgctgattc tcgccgcgat cccggaactt gccacctggc ttccgggaaa gctctacggc
      961 cggtagcgca ggccgcaggg gcggtgatcg taaagcaagg tggattattc atgaacggtg
     1021 agtgtggtgg tttgagaatg ccgccccggc gggggagggc ctttcaggaa cgccgtgaac
     1081 ccatccctcc cgccgggcgc tccgctggca ccgtcgtaaa ccccgttcat ggaggaggcg
     1141 tccacccacc tcaaaatacc ccgttcccgc gtcacagctc gcgcgtattc aggtacgcga
     1201 gctcagttat ccgctggttc tccgcggact tctcgagaaa gccgaaaaac gattcctgca
     1261 ggcgcttcgc ccacggatcg cgtgccgaaa gctcatcgac gatctcgccg ctcagccgtt
     1321 tcagcagcga gaggacgtca tcgggcagtt gcaggatctc gacgttggga tcgtccgcca
     1381 gctgggcgag ggccattgcg ttgccgtagg tgaattccgc ctgcatgtcc atgttggccg
     1441 cctggcagga ggcggtgacg atctcctgca ggtcttccgg aagggactcc cacgcgtcct
     1501 tgttgaccag gcactctatc atcggccccg gctcctgcca gccgggatag tagtagtagc
     1561 gggcgacctt gtgcaggccg agtgagacgt cgttgtaggg gcccacccat tccgccgcgt
     1621 caatggcccc ggtctgcaag gcggtgaaga cttcggatgc ggggacggtc acctgggtcg
     1681 cgcccgcgcg ctgcagcact tcgcctccga tacccggcat gcgcatcttc agtcctcgaa
     1741 ggtccgcgac agaattgatc ttcttgttga accagcccgc catctgcacg ccggtctggc
     1801 cggttgggaa ggggacaatg ttgaacggcg cgtacagctc ccgccagagc tcgaggccgc
     1861 cgccgtaata gagccaacca ttcgtttcgg tggcgttctg cccgaaggga atcgcggtga
     1921 agtattgtgc cgctggtacc ttgcctcgat gatagtaaga cgcatcgtgg cccatctgtg
     1981 cagtgccgcg gctgacggcg tcgaacacct cgagtgccgg tacgagttca ccacccgcgt
     2041 acaccttgat gttgagccgg ccgccggaag cccggccaat gcgtcgggcg agattaatgg
     2101 ccccggtccc gaggccgggc aatcccggtg gccaggaggt cacgagattc caggaaaagc
     2161 tttcgctgaa tcgttcctcc gggccgtccg ggccggcggc cttgtccccg cacgcggtaa
     2221 cgccagcggc gagagccagc ccgccgacga attgccttct tttcatgggc ttgcctttga
     2281 gcacttgtgg gccggtccga cacctggcgc tagtgtagaa tggcgagagg ccatagcaaa
     2341 gctcttttct agccgcacaa gtgtccactt aacgccaaaa tatccggctt gttacctaat
     2401 atttcccact tttgaggatt tcgttagaag gtggatggca tccctccaat tgcggccaat
     2461 atataggatc ttgtgggcag ccaacagtag ctgctgggga aatgaccccg gtctttcgac
     2521 cggggtcgcg ccttccctgg ctccgggcga agggcaaggc cggcaagccc tccatggcca
     2581 acccgttccg cgtttccggc gggacgtgac gcccggcttt gccagagtgc cacgggcagc
     2641 caaatgcttg cggtgggtgt tattgcatga tgggttaaca aactaagagc cggcatggcc
     2701 ttctcgccat gccagtggag tggaaatgat cggcttgctg cagcgcgtga ccgaagccag
     2761 cgtcactgtc gagggccgtg tcgtcgccag cattgggcgg ggactcctgg ttctggtggc
     2821 ggtaacacgc gaggacaccg aggaagcggt gtccaggctg atggaacgaa tactgacata
     2881 ccgggtcttt ccagatgatt gcaagcgcat gaacctgtgt ctggaggagc tggggctgga
     2941 cctgctcctg gtaccgcagt tcacgctggc ggccgacacc cgcaagggcc gcagggcaag
     3001 cttcacggga gcggccgaac ctgaaacggg cgcccggttt ttcgacctga tggtgaagct
     3061 ggcggcagaa cgcctgtccc gcgtcgagag tgggatattc ggcgccgata tgcaggtcgc
     3121 gctggtcaac gatgggcccg tgaccttctg gctcgaatcc tgaaatcttt aaggcaccgg
     3181 ttcggt
//
LOCUS       DASUOM010000284         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_969135,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000284 DASUOM010000000
VERSION     DASUOM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969135"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..667)
                     /locus_tag="VFY27_06005"
     CDS             complement(155..667)
                     /locus_tag="VFY27_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX5765103.1"
                     /translation="MQALIAKHMGLWAVCCLGLVAAANAEHHEKGNMKTNDIVDTAVG
                     AGQFNTLAAALKSANLVDTLKGEGPFTVFAPTDAAFAKLPEGTLENLLKPENREQLSA
                     ILTYHVVPGEVYAADVLRMTSATTVNGSDITIEADKGAVRIDGANVVQADIRASNGVI
                     HVIDAVILPN"
     gene            1214..2641
                     /locus_tag="VFY27_06010"
     CDS             1214..2641
                     /locus_tag="VFY27_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5765104.1"
                     /translation="MDRTSALQREACALFAISMPLMAAYLAEMGMMITDMIIVGRLGS
                     AELAAVGLTADWFYVLLLIGMGTVSIVGVLAAQSLGSGDRRGAADAVGQGMIAASIMS
                     VPVMMAVWYLGPALELARLDAEIVRLVTDYSRPLTFGVLPVLWFTVLRNYVAALARAS
                     MVMLIMAAALFLNLALNYTLVYGKFGFPALGVTGAGIGTSVVNWLVFAALAAHLLLAP
                     YFRDFRPRLVPGAVDAQVLRDIFRLGLPVTGAQLLAGGMFTFAAVLVGLLSPDVLAAQ
                     QVVYTVIYVALSASLAVGDAVRVRVAYGIGFGSPAGARGAAMIGCAMAAVITATASGA
                     LWLFPEVIVSIFLDTAEAANAGVLLIAVSLSIYAGVFQLIDGVLIVLANALRGLRDTR
                     SPMWIMLAGYWLVGIGTGALLCFPLGYGAAGLWWGLILGVVAAIALMASRFRRRMAEA
                     ELHLKSSSLRSDTLRDAFARAPREP"
BASE COUNT          501 a          848 c          886 g          616 t
ORIGIN      
        1 gtgtgttcga cgtagtctga gagcgccgtc aacgcaacaa aaaggggcag gtcatgccac
       61 agcaaattcc gcgagcggct tcggaccgaa tcgctagagg atatatagat cgtaaatcgc
      121 tcgacggcac gtccttgtgc cgccgaagac tctcctagtt aggcaggata acggcgtcga
      181 ttacgtggat cacgccattg ctcgcacgaa tgtccgcttg gacgacattc gcgccgtcga
      241 tacggacagc acccttatcg gcctcgatcg ttatgtcaga accattgacg gtggttgcgg
      301 atgtcattct gagcacgtca gcggcataca cttcaccagg cacgacgtgg taggtcagga
      361 tcgctgacag ctgttcgcga ttctcgggct tcagcaggtt ttcgagcgtt ccttcgggca
      421 acttggcaaa tgccgcatcc gtcggtgcaa aaacggtgaa aggaccttct cccttcagcg
      481 tatccacaag gttcgccgac ttcagtgcgg cagccagcgt attgaactgc ccagcaccga
      541 ccgcggtatc gacaatgtca ttcgtcttca tgttgccctt ctcgtggtgc tctgcgttag
      601 ctgcggccac cagacccagg cagcacacag cccaaagtcc catatgtttt gcgattagtg
      661 cttgcatttg cttatccttc aaagatgatg agtgaacgct gtcaatgtaa cggtaagcaa
      721 tcgcgcaatg gtgtcgaatg tgtatgcaaa cgtaactgtt tgtttctctc tgatcgagaa
      781 gctcgctata agttagtcag ttgaagcact ttgctagtag tcgaaggttc ggcctggagt
      841 gaattggcct agtcaacggc agccaggatc ccctcgtcgc cgcgaaggcg tagcggagtc
      901 cgcaatcagt cgccctcccg atccttatgc gtcaatcaca gagacgcaat agagtttctg
      961 gtcagcacgg cggaggagat cggcttcaac cgttacacaa tcctcaatct ccatgcgctg
     1021 ctagccaaca acttgctcgc cgactccgac gtcgcgggcc gactccgtca tataggtgtc
     1081 cgtatcgagc gctcttctcg ggtcgagagc tcccgtcgac ctccaacacc actttgtgag
     1141 agcgcctgaa tccattctgt tccttggcga tacactggcg actgaacact cgtcgcatat
     1201 tcaggggaac ccgttggatc gtacatccgc attgcagcgg gaagcgtgcg cgctgttcgc
     1261 gatcagcatg cccctgatgg ctgcctatct cgccgagatg ggcatgatga tcaccgacat
     1321 gatcattgtg gggcggcttg gcagcgccga gctcgccgct gtcggactga cagcggactg
     1381 gttctacgtg ctgctgctga tcggcatggg cactgtctcg atcgtgggcg tccttgccgc
     1441 ccagagcctg ggatcgggcg accggcgcgg ggcggccgat gccgtcgggc agggcatgat
     1501 cgcggcatcc atcatgagcg tcccggtcat gatggcggtg tggtatctcg gccccgcgct
     1561 cgagctggcg cggctggacg cggagatcgt gcgcctcgtg accgactact cccggccgct
     1621 gacctttggc gtactgccgg tcctgtggtt caccgtactg cgcaactacg tggcagcgct
     1681 cgcccgcgca tccatggtca tgctgatcat ggcggcggcg ctgttcctga acctcgcgct
     1741 caactacacc ctcgtgtacg gcaagttcgg tttccccgcg ctcggcgtga ccggggcggg
     1801 catcgggacg agcgtcgtca actggctggt gttcgcggca ctggccgcgc acctgctgct
     1861 cgcaccgtac ttccgggact tcaggccgag gctcgtcccg ggtgccgtcg acgcgcaggt
     1921 gctgcgggac atcttccgcc ttgggctgcc ggtcacgggc gcacagctac tcgccggcgg
     1981 catgttcacg tttgctgccg tgttggtcgg gctgttaagc ccggacgtgc ttgctgcgca
     2041 gcaggtcgtg tacaccgtga tctatgtggc gttgagcgca tcccttgcgg tcggcgatgc
     2101 cgtccgggtg cgggtggcat acggtatcgg gttcggttcg cccgccgggg cgcggggcgc
     2161 ggcgatgatc ggctgcgcca tggctgcggt catcacggca acggcatccg gcgccttgtg
     2221 gctgttcccc gaggtgatcg tcagcatttt cctcgacacc gccgaagcgg cgaatgccgg
     2281 cgtccttctg atcgcggtca gcctgtcgat atacgcaggc gtgttccagc tgattgacgg
     2341 ggtgttgatc gtgcttgcga atgcattgcg cggcctgcgg gacacgcggt cgccaatgtg
     2401 gatcatgctg gccggatact ggctcgtcgg catcggaacg ggcgccctgc tgtgctttcc
     2461 gctcggctat ggcgcggccg ggctgtggtg gggcctaatc ctgggggtgg tcgccgccat
     2521 tgcgctgatg gcaagccggt ttcgacgccg catggcggag gctgagctgc atttgaagtc
     2581 atcaagcctt cgctctgaca cattgcgaga tgcattcgca cgagctcctc gggagccttg
     2641 acgagccttt gcggaagcat cgagacgaat atagatgcct ggaaggcagg gtaatcgagc
     2701 gatattgaat atcacacgag gttcggcatc ggctgcccag taaagccgct cgtacaagga
     2761 gccgcgacgt cggacagcgc gtcaacacga tggatgacgt aaccgatgcc caagtctccg
     2821 ccgtgacgat cagttcagcg ccgcccgcgc g
//
LOCUS       DASUOM010000285         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_611003,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000285 DASUOM010000000
VERSION     DASUOM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611003"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFY27_06015"
     CDS             <1..736
                     /locus_tag="VFY27_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5765105.1"
                     /translation="GELHLSILIENMRREGYELSVSRPEVIMQEADGEVLEPWEQLTV
                     DLDEAYQGAVMSRLADRKGELLDIMPDGKGRVRMDYRIPTRGLIGFRTEFLTATSGTG
                     LIYHVFEKYAKRIPGAIAQRNNGVLVSMVAGKALAYALYNLQERGRLFIGHGDALYEG
                     MIVGIHSRQNDLSVNPTKGKQLTNIRAAGSDENIVLTPPIRLSLEQALEFIDDDELLE
                     VTPLNLRMRKKFLREADRKRESRLQA"
     gene            733..1743
                     /locus_tag="VFY27_06020"
     CDS             733..1743
                     /locus_tag="VFY27_06020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5765106.1"
                     /translation="MSEPAGPLDPVLAYAELRPDDILAALEALGFHCDGRFLALNSYE
                     NRVYRVGIEDGAPVVAKFYRPARWSDEAILEEHRFTAELAEQEIPVVAPLAIDGHTLH
                     HCGPFRVAVSPWRGGRAPELDEMAILGQLGRLVGRMHLQGEEHPFEHRPVLDVQSYGY
                     ASRDFLLDNNFIPDNLAAVYEGVVEDLLDRVCESFERAGNARTIRLHGDFHPGNVLIS
                     ESGQLHIVDFDDARSGPAVQDLWMFLSGDREEQTPQLAALLEGYEEFRRFDARELHLV
                     EALRTLRIMHYAAWIARRWEDPAFKIAFPWFDSPRYWDEHLLSLREQAALVTELPLEW
                     RG"
     gene            1981..>2255
                     /locus_tag="VFY27_06025"
     CDS             1981..>2255
                     /locus_tag="VFY27_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765107.1"
                     /translation="MKDPTIPDPLIDKMPLSVAARFPEAFEHFVHEEQVIGKKKWTID
                     MGRANSMLFEKLFGSMFTEFQVIPADAEAADFGVDALIEPSIDAFEFS"
BASE COUNT          454 a          667 c          707 g          427 t
ORIGIN      
        1 aggcgagctg cacctgtcga tcctgatcga gaacatgcgc cgggaaggct acgagctgtc
       61 ggtttcgcgg cccgaggtga tcatgcagga agcggacggc gaggtcctgg aaccgtggga
      121 gcagctgacg gtggacctcg atgaggcgta ccagggcgcc gtgatgtcgc gactggccga
      181 tcgcaagggg gagctgctcg acataatgcc cgacggcaaa ggccgtgtgc gcatggacta
      241 ccgcattcca acccggggac ttatcggctt tcgcaccgag ttcctcacgg caacttcagg
      301 caccggcctc atttatcatg tcttcgaaaa gtatgcgaag cgaattccgg gcgcaattgc
      361 ccagcgcaac aacggggtgc tggtatcgat ggttgcaggc aaggcgttgg cgtatgccct
      421 gtacaacctg caggagcgcg gcaggctgtt catcggccac ggcgacgcgc tttacgaggg
      481 catgatcgtc ggtatccaca gccgtcaaaa cgacctgtcc gtcaatccca ccaagggcaa
      541 gcagctgact aacatccggg cggccggcag cgacgagaac atcgtgctca ccccgccgat
      601 ccgcttgtcc ctggagcagg cgctggagtt catagatgat gatgaactac tcgaagttac
      661 accgttaaat ctgcgtatga gaaagaaatt tctaagagaa gccgaccgga aaagggaatc
      721 ccggctgcag gcatgagtga gcccgcagga ccactggacc cggtgctggc gtacgcggaa
      781 ctgcgtccgg acgacatcct ggcagcactt gaagccctgg gctttcactg tgacgggcgc
      841 tttctcgccc tgaacagcta cgagaaccgc gtgtaccgcg tcggcatcga ggatggagcc
      901 cccgtggtgg ctaaatttta ccgtccggcc cgctggagcg acgaagcaat cctcgaggag
      961 catcggttca ccgccgaact cgccgaacag gagattcccg tagtcgcgcc gctggcgatc
     1021 gacgggcaca ccctgcacca ctgcggcccg ttccgggttg cggtttcccc ctggcggggc
     1081 ggccgcgccc cggaactgga cgagatggca atccttgggc agctaggccg cctggtgggt
     1141 cggatgcacc tgcagggcga agagcacccg ttcgagcacc ggccggttct ggacgtacag
     1201 tcctatggat acgcttcccg cgacttcctg ctggacaaca acttcatacc ggacaacctg
     1261 gcagccgtct acgagggggt cgttgaggac ctgctggacc gcgtctgcga atccttcgag
     1321 cgcgccggca atgcaaggac gatccgcctt cacggcgact ttcacccggg caacgtgctg
     1381 atttcggaaa gcgggcagct tcacatcgtg gacttcgacg acgcgcggtc cggtcccgcg
     1441 gtccaggatc tctggatgtt tctctcgggg gatcgcgagg agcagacgcc gcagctcgcg
     1501 gcgctgctcg aaggctacga agaattccgg cgcttcgacg cgcgcgagct gcaccttgta
     1561 gaagcgctac gcacgcttcg cattatgcac tacgccgcct ggatcgcgcg ccggtgggag
     1621 gatcctgcgt tcaagatcgc cttcccatgg ttcgattcgc cgcggtactg ggatgagcac
     1681 ctgctgagcc ttcgcgaaca ggcggcgcta gtcacggaac tcccgctgga atggcgcgga
     1741 tgaagtggcg tgaaggcgaa atgcaccagt tgcgctgctt ataaaccggg tctaaagtga
     1801 caggcgttat cttctacttg ctctggcgga actctggcgt gcgcccgggc tcagagtgtt
     1861 gaataaagcg gatttctccc gcccttccca ggactggcca aattgatcgc agcaaggctc
     1921 aagttcgttt cggttgcaat aattattgca gcgctgagcg gctgctcgag ccaggtaacc
     1981 ttgaaggatc cgacaattcc cgatccgctg atcgacaaaa tgccactgtc ggtcgcggca
     2041 cggtttccgg aagctttcga gcatttcgtc cacgaagaac aggttatcgg caagaagaaa
     2101 tggaccatcg acatgggccg ggccaacagc atgctgttcg aaaagctgtt tggctcgatg
     2161 tttacggagt tccaggtgat cccggcggat gcggaagcag ccgatttcgg tgtcgacgcg
     2221 ctgatcgagc cgagcatcga cgccttcgag ttctc
//
LOCUS       DASUOM010000286         7494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1125969,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000286 DASUOM010000000
VERSION     DASUOM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125969"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VFY27_06030"
     CDS             <1..1482
                     /locus_tag="VFY27_06030"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX5765108.1"
                     /translation="IWRYEPVQNAEETIPVMCCDTVSRGLAYADGKIFLQQADTTLVA
                     LDAKTGKEVWKVKNGDPKKGETNTNAPVVVKDKVITGISGGEFGVRGFVVAYNIKDGS
                     QAWKAYSVGPDEEMLVDPRRTTHLGKPVGRNSSIDSWEGDQWEIGGGTTWGWFTYDPA
                     TNLVYYGSGNPGTWNPVVRPGDNRWSMTIWARDMDTGVAKWVYQMTPHDEWDFDGVNE
                     MILADLEIDGKNRKTLVHFDRNGFGYTLDRVTGELLVAEKYDPAVNWATHVDMKTGRP
                     EVVAQYSTHQNGEDTNTENVCPAALGSKDQQPAAFSPKTGLFYVPTNHVCMDYEPFEV
                     EYVAGQPYVGATLSMFPAGQVTGDGSTNLGNFIAWDATTGEIKWSVPEPFSVWSGALA
                     TAGDVVFYGTLEGYLKAVSAESGEELYAFKTPSGIIGNVNTWTYNGKQYVGVLSGIGG
                     WAGIGMAAGLTGDTEGLGAVGAYKSLDKYTQLGGVLTVFALPD"
     gene            1642..2127
                     /locus_tag="VFY27_06035"
     CDS             1642..2127
                     /locus_tag="VFY27_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5765109.1"
                     /translation="MKPVAWALLAGMPLACVSDDSAGPRPANAAAMAAEPAPETAIPA
                     GGDQKFYDVDCSQVDESGNKVCITDRETYVGWRTFHATCHACHGQNAVGSSFAPSLLK
                     DIPKERFVDVVTNGYTGQIGVMPAWGENPNVKDFIDELYAYQQARADGVLLPGRPTRK
                     D"
     gene            2129..2989
                     /locus_tag="VFY27_06040"
     CDS             2129..2989
                     /locus_tag="VFY27_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEX5765110.1"
                     /translation="MHKYGLKVALLIAVAILAQPAAALEREAFRVCADPNNLPFSHKN
                     LKGFENQLAELWADHLGLEVQYTWFPQRIGFVRNTLGSKNEHNEYKCDVVMGVAEGFD
                     QLMTTKPYYRSTYALVYIKGRELDDVRTGRDLLNLDAGRKANLRIGAFDKTPGPTWLQ
                     RHNMLDQMRPYQAMSGDPEDYPGEILEQALVDGEVDAAIIWGPIAGYFSKQAQDVEMV
                     VIPLESEPGVQFDYGISAGVRRGDDEGKAQLEQLMNETDQQIQALLLSYNVPLLPPEV
                     ASAKRADDDD"
     gene            3474..4700
                     /locus_tag="VFY27_06045"
     CDS             3474..4700
                     /locus_tag="VFY27_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5765111.1"
                     /translation="MVRRFRSAFILWIALHAGLALGQEDNRIDAGADGFSLRSVDGDW
                     SLRLRGLLQVEGRYFRDDSASGDDNEWVLRRIRPTLEGQFGKRISFRIMPDFGLGDSQ
                     LIDSWIETRLNAGLTLRAGKFKPPVGLERLQSASDLRIVERSFVTDLVPNRDIGVQLS
                     GGGPRLSWAAGIFNGVDDGRSADQDNDGQPDFALRLFSEPLQKDSDTTLGIGIGVTFG
                     NTDGTAVTPLLSGYRSPGANTVFEYRSGADGTFADGDRLRISPQFYWYRGPVGLLGEW
                     VRVRQDVRRAGTGFDRSGPLDHEAWQVTAEWYVTGQAAGYRDADGSAGAVQFVARLAA
                     LKIDQDSFIGGATSFADSAAATEQALTQAVGVNWFPVAGLKAALAIHHSTFRGGAPTG
                     DRPDEDVIFLRLQHAF"
     gene            4821..6614
                     /locus_tag="VFY27_06050"
     CDS             4821..6614
                     /locus_tag="VFY27_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765112.1"
                     /translation="MSISLEQVSKSYEEGAAVSDVTVDIADGEFFVLLGPSGSGKSTL
                     LRAIAGLTTIDHGRIVLHGRDVSNVNAREREVGFVFQNYALFRHMTLADNIEFALCAR
                     RVPAARRKVRRRELLKLVSLEGFDDRYPSELSGGQQQRVAVARALAHEPRVLLLDEPF
                     GALDAKIREELRRAIREIQRAVGITTILVTHDQEEAFSMADRIGVMDRGRLQEVGEPR
                     VLYRQPATRFVATFLGAANLLLGNYGYRDVRLGNSWFPVPRWSRSLCSGDEATIIVRP
                     EDVELAHREDQLTVPAAGLAEVEELQFVGSIERLRLTIPASETLASALRPGASSFLLE
                     AARTARAVEDFPLSRGQKVWVGFKGMHALPTPVSSLRLLASSDEAFAALSGSALVREL
                     GERMRIEPVRIDAGTTKEASLRGMPILALQRPADLSEPLELLEEGARQVLVLLPGNRR
                     ADRMLILVEPSAAARDASLAAVASLARHLTIDVTMLVHVDEKSGRGARYRELLDLRNT
                     SLRSHGLDIRTETYHGSLRDAVEARQSSSGQVLLVIGLATSINNTRLIGEVKEVLEMS
                     PPAALILVSGRADRDLRETARVSPRAAAAAR"
     gene            6653..>7494
                     /locus_tag="VFY27_06055"
     CDS             6653..>7494
                     /locus_tag="VFY27_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5765113.1"
                     /translation="MASLVVATLVAGCGKQDTSSAPGGSTAARPVQLLNVSYDPTREM
                     YREFNEAFAAHWLAETGQNVSVQQSHAGSGSQARAVIDGLDADVVTLALAYDIEAIRL
                     HGQRLQPGWQNRLPNNSAPYTSTIVFLVRKGNPKDIRDWPDLVQPGVQVITPNPKTSG
                     GARWNYLAAWGYELEQALGGDIKKLGTASPEEVERAQARAERFVGELFRNVPVLDRGA
                     RAATNTFVQRGIGDVLLAWENEALLAIDELGADTLDIVVPSVSILAEPTVAIVDSVVD
                     RKRTR"
BASE COUNT         1502 a         2263 c         2298 g         1431 t
ORIGIN      
        1 atctggcgat acgaacctgt gcagaacgcc gaagaaacga taccggtaat gtgttgcgat
       61 accgtttccc gcggcctcgc ctacgcggat ggcaagatct tccttcagca ggcggacacg
      121 acgctcgtcg cgctggacgc caagacaggc aaggaagtgt ggaaggtcaa gaacggcgat
      181 ccgaagaaag gcgaaaccaa caccaacgct ccggtagtcg tgaaagacaa ggtcatcacc
      241 ggcatcagcg gtggtgaatt cggggtacgt ggtttcgtgg tcgcgtacaa catcaaggat
      301 ggttcgcaag cgtggaaggc ctatagcgtc gggccggacg aggaaatgct ggtcgacccg
      361 cggcgaacca ctcacctcgg caagcctgta ggccgcaatt ccagcatcga cagctgggaa
      421 ggcgaccagt gggagatcgg tggcggcacc acctggggct ggtttactta cgacccggca
      481 acgaacctcg tgtactacgg ttcgggaaat ccgggtacct ggaacccggt cgtacgcccc
      541 ggtgacaacc gctggtcgat gaccatctgg gcacgcgata tggataccgg cgtagccaag
      601 tgggtgtacc agatgacgcc tcacgacgag tgggatttcg atggcgtcaa cgagatgatt
      661 ctcgccgatc tggaaataga cggtaagaat cgcaagaccc tggtgcattt cgatcgtaac
      721 ggcttcggct acacgctgga ccgggttacc ggtgaattgc ttgtggctga gaaatacgac
      781 ccggctgtga actgggcaac ccatgtcgac atgaagacag gtcgacccga ggttgtggcg
      841 caatacagca cccaccagaa tggcgaagac accaacaccg agaacgtatg cccggctgcg
      901 cttgggtcca aggaccagca gcctgctgca ttttcaccga agaccggtct tttctatgtg
      961 cccaccaacc atgtgtgcat ggattacgaa ccgttcgaag tggaatacgt cgcgggccag
     1021 ccgtacgttg gcgcaacgct gagcatgttc ccggcaggcc aggtcactgg cgatggcagc
     1081 acgaacctgg gcaacttcat cgcctgggat gccacgacag gcgagatcaa atggtcggtc
     1141 ccggaacctt tctccgtctg gagcggggcc ctggctacgg ccggcgatgt ggtcttctat
     1201 ggcacgcttg aaggctatct gaaagccgtc agtgctgaaa gcggagaaga gctgtacgcc
     1261 ttcaaaacgc cttcaggcat catcggcaac gtcaacacct ggacatacaa cggcaaacaa
     1321 tatgtcggcg tcctgtccgg aatcggcgga tgggcaggta tcggcatggc tgccggcctc
     1381 accggcgaca ccgaaggctt gggcgctgtt ggtgcgtaca agtccctgga caagtacacg
     1441 cagctcggtg gcgtactgac ggtgtttgca ttgcccgact agcacgactc ctcaaggggt
     1501 ggtgcgacaa gatcgcagga tagttaaatg cttgttaacc cccgactgac cagtgcagtc
     1561 gggggttttt tgatgatgga ataatcagag agaacggtgc tcatgccata tcgcttgata
     1621 cacagccgga ttcggaccgc gttgaaacct gttgcctggg cgctgctggc cggcatgcca
     1681 cttgcctgcg tttcggacga ctctgccggg cctcgaccag ccaatgcggc cgccatggct
     1741 gctgaacctg ctcccgaaac cgcgatcccg gccggcggcg accagaagtt ttacgacgtg
     1801 gactgcagcc aggtcgacga aagcggcaac aaggtttgca ttaccgacag ggaaacctac
     1861 gtcggctggc gcacatttca tgcaacctgc cacgcctgtc atggtcaaaa tgcggtcggg
     1921 agttcctttg cacccagtct gctgaaggac atcccgaagg aacgatttgt cgacgtggtg
     1981 acgaacggct ataccggcca gatcggcgtc atgccggctt ggggtgaaaa ccctaacgtg
     2041 aaggacttca tcgatgagct ttatgcatat cagcaggccc gcgccgacgg cgtgctactg
     2101 cccggacgcc cgacgagaaa ggactgacgt gcacaaatat ggacttaagg tcgctctgtt
     2161 aatcgctgtt gcgatccttg cgcagccggc agcggccctg gagcgcgaag cattccgcgt
     2221 gtgcgccgat cccaacaacc tccccttctc gcacaagaac ctgaagggat tcgaaaacca
     2281 gcttgcggag ctgtgggccg accacctcgg cctggaggtg cagtacacct ggttcccgca
     2341 gcgcattggc ttcgtccgca acaccctggg aagcaagaac gagcacaacg agtacaagtg
     2401 cgacgtggtc atgggagttg ccgaaggttt cgaccagctg atgacgacga agccgtacta
     2461 ccgctcgacc tacgcgctgg tctatataaa gggccgagag ctcgacgacg tccgtacagg
     2521 acgcgacttg ctgaacctgg atgccgggcg gaaagcaaac ttgaggatcg gtgccttcga
     2581 caagacgccc ggccccacct ggctgcagcg gcacaacatg ctcgaccaga tgaggcccta
     2641 ccaggcgatg tccggcgatc ccgaagatta tcccggcgag attctcgagc aggcgctggt
     2701 cgacggtgag gtggatgcag cgattatctg gggtcccatt gccgggtatt tctccaaaca
     2761 agcgcaggat gtcgagatgg tcgtcattcc gctcgaatcg gaaccgggcg tccagttcga
     2821 ctatgggatt tcggcgggcg ttcgtcgcgg cgacgacgag gggaaggcgc agctcgagca
     2881 gctcatgaac gaaaccgacc agcagatcca ggccttgctc ctgagctata acgtgccgct
     2941 attgccgccg gaagtggcgt cggcgaagcg agccgacgac gacgattgat ttgttgcggg
     3001 gcctgacggc actgttcggc ccccctgggt gtgaacccgc tgttgctcgg cgctccccga
     3061 cgtaatgttc cttgcagcct tgcgcatcac ggcagcccgc aggcgtgccg tgatgtgcat
     3121 gagacctggc gcttcagaat cctcaacacc acgcatcgtt tcctgacctg gttttcgcgc
     3181 gtgctgtcag atgcagctgc ctgtcaccat gcaaccagtc caacgcgatg gtttgcagcc
     3241 cgccctttcc ctgggacgat gccggctcta accatcggcc gaaccttcat aacctgagcg
     3301 gcacgaccga cgaagcaagc tcagacaagg cacgccacat cttgtgtcgc ccactctccg
     3361 cgaaacgatc ctggccacca attgttattt cgccgtcacc ggaacgagac cgacacggtt
     3421 accgggcagc actaacattt gcattccgtt ctccaaaatc caggcgatgt gtaatggtca
     3481 gaagatttcg gtctgctttc attttgtgga ttgctctcca tgcgggcctg gcactcgggc
     3541 aggaagacaa ccgcatcgac gcgggcgcgg acggtttttc gctacgctcc gtggatggcg
     3601 actggtccct tcgcctgcgg ggtctgctac aagtggaagg tcgctacttc agggatgact
     3661 ccgcttccgg cgacgataac gagtgggtgc tgcgtcgcat ccggccaacg ctggaaggcc
     3721 agtttggcaa gcgaatctcc tttcgcatca tgccggactt tggcctgggc gacagccagc
     3781 tgatcgattc ctggatcgag acccggctta atgccggact tacgctgcgc gcaggcaagt
     3841 tcaagccgcc cgtcggactt gaacgtctgc agtccgccag tgacctgcgc attgtcgagc
     3901 gatcgtttgt tacggatctg gtaccgaatc gggatatcgg cgtgcaactt tcgggtggcg
     3961 gaccgcgttt gagctgggct gccggcattt tcaacggtgt agacgacgga cgctccgccg
     4021 accaggacaa cgacgggcaa ccggactttg ccttacgctt gttctccgag ccgctgcaaa
     4081 aggacagcga tacaacactc ggtatcggaa ttggcgtcac gttcggcaat actgacggca
     4141 ccgctgttac gccattgctc tcgggttacc gctcgcccgg cgcgaatacg gtattcgaat
     4201 atcgcagcgg tgctgacggc acgtttgccg atggcgaccg gctcaggata tcccctcagt
     4261 tctactggta tcgtgggccg gtcggactgc tcggcgaatg ggtacgcgtc cggcaggacg
     4321 tacggcgagc aggcaccggc tttgaccgat caggaccgct tgaccacgaa gcctggcagg
     4381 tcaccgccga gtggtatgtc accgggcagg ctgcagggta tcgtgacgct gacggctccg
     4441 cgggagcggt gcagttcgtg gcgcgactcg ccgcactcaa aattgaccag gactcgttca
     4501 ttggcggcgc cacgtcgttc gccgattccg ccgctgccac ggaacaggcc ctgacgcagg
     4561 cggttggcgt caactggttc cctgttgccg ggttgaaagc agctctggcg attcaccaca
     4621 gcaccttccg gggcggcgcc ccaaccggcg accgccccga tgaggacgtg atcttcctgc
     4681 gcctgcagca cgcgttctag gagcagcttt ccagcgcctg cgaaaatgtc accgcgttgt
     4741 aaccgggccg gcaccgaccc ggttaccggc ggtggctagc atcaggctga cgactcctat
     4801 cgattaccca tggggcacgc atgtcaatct cactggaaca agtctcgaaa tcctacgagg
     4861 aaggcgcggc ggtcagcgat gtcacggtcg atatcgctga tggtgagttc ttcgtgcttc
     4921 tcggaccgtc aggcagcggc aagagcaccc tcttacgggc gatcgccggc ctcacgacga
     4981 tcgaccacgg gcgaatcgtg cttcacggcc gcgacgtttc gaacgtaaac gcccgcgaac
     5041 gggaagtcgg cttcgtattc cagaactatg ccctgttccg gcatatgacc ctggcagaca
     5101 acatcgagtt cgcgctgtgc gcgcgtcgcg tgcccgccgc caggcgcaag gtccgccggc
     5161 gcgagctatt gaagctcgta tccctggagg gattcgacga ccgctatcct tccgagctgt
     5221 cgggcggcca gcagcagcgg gttgccgtgg cgcgcgcatt ggcgcacgaa ccccgcgtgc
     5281 tgctgctgga tgagcccttc ggcgctctcg acgcgaagat tcgcgaggag ctgcgtcgcg
     5341 ctatccgcga aatccaacgg gctgtcggca tcacgacaat actcgttacg cacgaccagg
     5401 aagaagcctt cagcatggct gacagaatcg gtgtcatgga tcgcggacgt ctccaggagg
     5461 taggcgaacc gcgcgtactc taccggcagc cggcgacgcg tttcgtcgcg acctttctcg
     5521 gggccgcgaa cctgctgctt ggcaattacg ggtatcgcga cgtccggctc ggcaattcat
     5581 ggttccccgt gcctcgatgg tcgcgctcac tatgcagcgg cgacgaagcc acaattatcg
     5641 ttcggccgga agacgtggaa ctggcccacc gcgaggacca gctcaccgtt cccgcggccg
     5701 ggcttgccga agtcgaagag ctgcaattcg tcgggtccat agagcgactg agactgacta
     5761 ttccggccag cgaaacgctg gccagcgcgc tgcgtccagg agccagttcg tttttgctgg
     5821 aggccgcgcg cactgcgcgc gcggtcgagg actttcccct gtcgcgaggg caaaaggtct
     5881 gggtgggttt caagggaatg cacgctttac ccacacctgt cagcagtttg cgtttgctgg
     5941 cgtcttccga cgaagccttt gcagcgctgt caggctccgc gcttgttcgc gaactcggtg
     6001 agcgcatgcg catcgaaccg gtccgcatcg acgccggcac gacgaaagag gcttcgctgc
     6061 gaggtatgcc gatactggct ctgcagcggc cggcagattt atccgagcca ctggagcttc
     6121 tcgaggaggg cgctcgccag gtgctggtcc tgttgcccgg aaacaggcgt gcggaccgca
     6181 tgttaatcct tgtcgagccg tctgccgccg cgcgcgatgc gagcctcgcg gcggtggcga
     6241 gtcttgcgcg ccatctgacg atcgatgtca cgatgctcgt gcatgtcgac gagaagtccg
     6301 gccgcggagc tcgctatcgg gaactcctcg acctgcgcaa cacttcccta aggtcacacg
     6361 gcctcgatat tcgcaccgag acgtatcacg ggtccctccg cgatgcggtc gaggcacgac
     6421 aatcttcttc cgggcaggta ctgctggtga tcggtctcgc gacgtcgatc aacaacactc
     6481 gtctgatcgg ggaggtcaag gaggtactcg aaatgtcacc accggccgca ttgattcttg
     6541 tcagcggtcg tgccgatcgc gacctgcgcg aaacagcgcg ggtatcccct cgagccgccg
     6601 ccgcggcgcg ctgatcacac cagaagcaat catgtctgta acacgcaaag tgttggcatc
     6661 gcttgtcgtt gcaaccctcg ttgctggatg cggaaaacag gatacgtctt ctgcgcccgg
     6721 cggttcgacg gctgcacgac cggtccagtt gctgaacgtt tcctacgatc cgacacgtga
     6781 gatgtaccgg gagtttaacg aggcgtttgc cgcgcactgg ctggctgaga ctggccagaa
     6841 cgtctccgtg cagcaatcgc atgcgggttc cggcagccag gcacgcgccg tcatcgacgg
     6901 tctcgatgca gacgtcgtca ctctggcgct cgcctacgac atcgaagcaa ttcgcctcca
     6961 cggccaacgg ctccagcccg gctggcagaa ccgactgccc aacaacagcg cgccttacac
     7021 atcgacgatt gtttttctgg tcagaaaggg aaacccaaag gatattcgcg actggcccga
     7081 cctggtgcag cccggcgtgc aagttatcac accgaatccg aaaacctcgg gcggcgcacg
     7141 ctggaactat cttgctgcgt ggggctacga actcgaacag gcgcttggcg gagatatcaa
     7201 aaagctcggc accgcatcac ccgaagaggt cgaacgcgcg caggcacgcg cggagcgctt
     7261 cgtcggagaa ttgttccgta atgtgccagt gctggatcgc ggcgcacgcg ccgccaccaa
     7321 caccttcgtg cagcgcggta taggcgatgt gctgctcgcg tgggagaacg aagctttgct
     7381 cgcaatcgat gaactcggcg ccgacacact tgatatcgtc gtgccgtcag tcagtattct
     7441 ggccgaaccg acggtcgcca ttgtcgactc ggtcgttgat cgcaaaagaa cgcg
//
LOCUS       DASUOM010000287         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_401696,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000287 DASUOM010000000
VERSION     DASUOM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401696"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFY27_06060"
     CDS             <1..648
                     /locus_tag="VFY27_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5765114.1"
                     /translation="DRSMAGMRKLIARSLTDVRTMAKPTARRRIVPVADFIAEIQLSA
                     SLEAREHGCAFVVEPVDASLVICVDRELLLSALGNLLQNAFKFTRSHTEVVLKTIAAG
                     DRVLIEVEDSCGGLPAGDVEEMFRPFTQNGHDKSGVGLGLVICRRSVEANGGTLRVRD
                     VPGSGCVFTIELPGHVSTKGAAEASQDPAKCASVQTQTPRSAQAACLITSEINNV"
     gene            complement(811..1491)
                     /locus_tag="VFY27_06065"
     CDS             complement(811..1491)
                     /locus_tag="VFY27_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5765115.1"
                     /translation="MPLYRNPRQRRYMRQARRPFSRLWRTFLTGLVAIIPLAVTGYVV
                     YWLFTAADSLFSGIGQALLPPGWYFPGLGLVAGVLAVLALGAVLNAWVFGRVMLTLGT
                     RLLERIPLIKTVYSGVRDLMLFVSRTPDDDSRHVVLVTLPGDIRLIGFITDSAPAQAI
                     PELTLEGETVLAVYLPMSYQIGGYALYLPERCLQRLEMSVEEGMRLVLTAGMNRPRTD
                     SPPTQVVA"
     gene            complement(1521..2384)
                     /locus_tag="VFY27_06070"
     CDS             complement(1521..2384)
                     /locus_tag="VFY27_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5765116.1"
                     /translation="MMDVSSLIKSRLKELGIGQKELAAAAEVTDSYVSQLLNRKKLPP
                     APDRTDIYEKMGRLLRLSNGELATLARAQRLEILKRGFEDRPPPLLKNVRELILRKCV
                     PEKQQQMRDIFEKQPFGELERLVTQILLDVVSKVARAELENREWLHDVAQLSDKSYEQ
                     MRVIILEFLDTDIFNVSHENCVSFLDPLIASWDIDLSTFDMEIAMNERLVPGKPKKFE
                     FVEKDSAALEVEPGFTEFLQDRTLSGDVTRDESEFLGRLRFRGKRPAPLYYYRELQNL
                     RDPLHFRAAEP"
BASE COUNT          537 a          739 c          742 g          529 t
ORIGIN      
        1 gatcgcagca tggcgggtat gcgcaaactc atagctcgct ctctcaccga tgtgcggacg
       61 atggcgaaac cgactgcgcg ccggcggata gtgccagttg cggatttcat tgccgagatc
      121 cagctttccg cctcactcga ggcaagagag cacgggtgtg ctttcgttgt cgagcccgtt
      181 gacgcatcgc tcgtgatctg cgtggatcgg gagctgctgc tttcggccct cggcaacttg
      241 ctgcaaaacg cgttcaagtt cacgcgctct cacaccgagg ttgtcctcaa gaccattgcg
      301 gccggcgatc gtgtcctgat cgaagtcgag gacagctgtg gcggattgcc ggcaggcgac
      361 gtagaggaga tgttcaggcc attcacgcaa aacggccacg acaaatccgg tgtcggcctg
      421 ggactggtga tctgtcggcg cagcgtcgag gcgaacggcg gaacgttgcg cgtccgggac
      481 gtgccgggtt cgggatgcgt attcacgatc gagttgccgg ggcacgtatc gacgaaaggc
      541 gctgccgaag cctcacagga ccccgctaag tgcgctagcg tacagaccca aacgccacgt
      601 tcagcgcaag ctgcctgtct tatcaccagt gaaataaata acgtttaacg taagaagttt
      661 gatagtcggc gccgcagtgg tggcgactgc cgcaggctgc gccagcatcc ggggcacgga
      721 cagcatctcg gacaccaccc gcaccggcac gatccataca gtaaatttcg aagagcatat
      781 gacaccgagt aacctgcggg ttcagccagg tcacgcaact acctgggtgg gcgggctgtc
      841 cgtgcgcggg cggttcatgc cggcggtcag taccaggcgc atgccctcct ccaccgacat
      901 ctccagcctc tggaggcagc gctccggaag atacagcgcg taacctccaa tctggtagct
      961 catgggcagg tacactgcaa gtacggtttc gccctccagt gtcaattccg gtatcgcctg
     1021 cgctggtgcc gagtcggtga tgaagccgat cagtcggatg tctcccggaa gcgtgacgag
     1081 aacgacatgc cggctatcat catccggcgt gcgggaaacg aacagcatca ggtcgcggac
     1141 accgctgtac acagtcttga tgagcggtat cctctccagg agccgggtac caagcgtgag
     1201 catcactcgg ccgaagaccc aggcattgag aacggcaccc agcgccagca ccgccagcac
     1261 gccggcgacg aggcctaacc cggggaagta ccagcctggt ggaagaagtg cctgtccaat
     1321 cccgctgaac agcgaatcgg cagcggtgaa cagccagtac accacgtatc cagtcaccgc
     1381 cagggggatt atggcaacca ggcccgtgag gaacgtgcgc cacagacgcg agaatggacg
     1441 acgggcttgc cgcatatatc gacgttgacg cgggtttcgg taaagcggca tgacatgcgc
     1501 tccgtggttt tggcgcgtgc ttacggttct gcagctcgaa aatgcaacgg gtccctcagg
     1561 ttctgcagct cacgataata atagagtggg gcaggccgtt tccccctgaa tcgcaacctg
     1621 cccaaaaatt ccgattcgtc acgggtgaca tcgccgctga gcgtccggtc ctgaagaaat
     1681 tcggtgaagc ccggctcgac ctcgagggcg gccgaatctt tctcgacgaa ttcgaacttc
     1741 ttcggttttc ctggaacgag tctttcattc atcgcaattt ccatatcgaa ggtcgagagg
     1801 tcgatgtccc agcttgcaat caaaggatcc aggaacgaaa cacagttctc atgcgaaacg
     1861 ttgaagatgt ctgtgtcgag gaattccagg atgatcacgc gcatctgctc atagctctta
     1921 tcgctgagct gtgcgacgtc gtggagccac tcgcgattct cgagctctgc tctggcaact
     1981 ttgctgacga catccaggag gatttgcgtg acgagccgct cgagttcccc gaagggctgc
     2041 ttctcgaata tgtcccgcat ttgctgctgc ttttccggaa cgcacttccg cagtatcagc
     2101 tctcgcacgt tcttcaacag tggcgggggc cggtcttcga acccgcgttt caatatttcc
     2161 aaccgctgtg cgcgagcgag tgtcgccagc tcgccgttcg agaggcgtag aagcctcccc
     2221 atcttctcgt agatatcggt ccgatccggg gcagggggca gtttcttccg gtttagcagc
     2281 tgggatacgt aggagtccgt cacctctgct gcggcggcca gttccttttg gccgatcccc
     2341 aactccttca gtcgcgattt tatgagcgac gaaacatcca tcatccgggc tcacctggat
     2401 tactgtattg gttaattgac cgaaatgatg tcagatagta cttgactgta caagtgaatt
     2461 aacccattat ggtaactgga ccgatgaggg cttccgacca atcaaccaag ttggttatct
     2521 gaagcgccct ttttggccga tcacaag
//
LOCUS       DASUOM010000288         6447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1032333,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000288 DASUOM010000000
VERSION     DASUOM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032333"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1725)
                     /locus_tag="VFY27_06075"
     CDS             complement(103..1725)
                     /locus_tag="VFY27_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5765117.1"
                     /translation="MTDVPIVVQQTHHEHDQWWKAGVIYQIYPRSFQDSKGDGIGDLH
                     GIAGRLDYLAWLGVDAIWISPIFPSPMADFGYDVADYRGVDSIFGTLEDFERLLAAAH
                     ARKIQVLLDFVPNHTSDQHPWFIEARRSRDSPMRDMYIWRDPAPGGGPPNNWLSDFGG
                     SAWEWDERTEQYYYHAYLRQQPDLNWRNPAVRAKMYDVLRYWLERGVDGFRVDVLWHL
                     VKDDQFRNNPPNPDWAPGMPPHKKVLQLYSADRPEVHEIVSEMRSVLAEYGDDRLLIG
                     EIYLPLARLVAYYGRDLGGAHVPFNFHLIQAAWHARSLQREIQEYEAAIPEGGWPNWV
                     LSNHDKPRIVARVGPAQARVAAMLLLTLRGTPTIYYGDEIGMDNVEILQSQVMDPFER
                     NVPGQGFGRDPQRTPMQWSRGLNAGFTSGSPWLPLSPKAHEINVDAESTNPASMLSLY
                     RRLLALRREHRALTVGRYQPVKASGDVLAFAREAARERFLVALNLGPAPAELPVPWRA
                     RVVLSTHNDRNGEKVRSSLLLRENEGILAVVQ"
     gene            complement(1718..2551)
                     /locus_tag="VFY27_06080"
     CDS             complement(1718..2551)
                     /locus_tag="VFY27_06080"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5765118.1"
                     /translation="MPTDVADAGAVEAAAEKAEEKFGAIDAWVNSAMVTVYSPLAEIT
                     AEEFRHVTEVTYLGQVYGTLSALRRMRSRNRGTIVNVSSALAYHGLPLQAAYCGAKFA
                     ARGFTESLRCELLHEGSDVRVTLVVLPAVNTPQFDWARNKMPNRPRPVPPVYQPEVIA
                     SSIVRAAETAPREMLIGRSSVQLVAGSMVAPGVVDSILAAKAYRGQQASEPAQTRPDN
                     LFEPAPGDFGSHGRFDEESSTSGGDIALGTARGVSAAAGLALLAGLAFAAYGMGRRHY
                     D"
     gene            complement(2541..2945)
                     /locus_tag="VFY27_06085"
     CDS             complement(2541..2945)
                     /locus_tag="VFY27_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5765119.1"
                     /translation="MGDRTPSPEERISDPWWKDVIIYAVDVERFCDSNGDGQGDFKGL
                     TSKLPYLADLGITCLWIVPFYPSSNRDNGYDITDYLRVDTRYGLFEDFLECLHTAGEF
                     GIRIIVDHASPDAWMRRSQRSKGRGRGVGSAH"
     gene            3074..3160
                     /locus_tag="VFY27_06090"
     tRNA            3074..3160
                     /locus_tag="VFY27_06090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3108..3110,aa:Leu,seq:taa)
     gene            complement(3198..3491)
                     /locus_tag="VFY27_06095"
     CDS             complement(3198..3491)
                     /locus_tag="VFY27_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5765120.1"
                     /translation="MSDLEEGAVSGIGTKYFKRPNLEKLVCEALATDQRVNASQIGVA
                     LDEATGDLTLFGTVPTKEQLQFAQDCAASVKGIHVVHSRLTVADGRAGSGTEA"
     gene            3820..4011
                     /locus_tag="VFY27_06100"
     CDS             3820..4011
                     /locus_tag="VFY27_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765121.1"
                     /translation="MKLADILKQNANRSMDVEMVNGATVTAGSWSIGDELVGAHKQKG
                     YTIYICLDKIVSIKVESAP"
     gene            complement(4117..5436)
                     /locus_tag="VFY27_06105"
     CDS             complement(4117..5436)
                     /locus_tag="VFY27_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5765122.1"
                     /translation="MDEALLEQARDHALRGGGSGMIIRGGKLVFSWGNHETLYDLKST
                     TKSIGVTALGLALQDGLVQLDDAAQMHLPGIGVPPESNTARAWLDDITVRHLATHSAG
                     FDKTGGYIDLLFEPGSMWAYSDGGSNWLADLLTVRFASDLKDLMFARVFQPLGLDDKD
                     ITWRNHNYRDDTIAGITRREFGSGIHADVDAMVRIGYLYLRMGSWEGQQILPRAFVEQ
                     AARPDPVLQGLPVHQEDDHPDASSHYGLLWWNNGDGRLANVPSDAFWSWGLRDSFIIV
                     IPSLDVVVSRAGNRWRSGWNSDYSVIEPFLEPIAQSVHDDGSTPDPGQNPVVTISSPA
                     DGASEPEGSTLHFVGSATDSEDGDLTSSLEWVSNQAGLVGRGGAFSTSTLPVGKHSIT
                     ASVTDSDGFSGTAAIELSVFESGEDQAAEEDPPPDEGSPPRRTPTYR"
     gene            6118..>6447
                     /locus_tag="VFY27_06110"
     CDS             6118..>6447
                     /locus_tag="VFY27_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765123.1"
                     /translation="MSFHQPEGKVPAETARELATAAILLEQTNTLLDRVGERKPPRPV
                     DDEKAASDAAPLDVRDEKIPQLIGDGDMSPQRVADHLAAIKSQKPLRKAQTWLWDAEH
                     LLDVLSVY"
BASE COUNT         1261 a         2012 c         1928 g         1246 t
ORIGIN      
        1 tggccatgac cgtttctcag gaattcaccg ggttgaacag tttcagatcc ccgaggctta
       61 gccggggatt gcgcttggga ccattctgac actcaccgcc tgttattgca cgacagccag
      121 gattccctcg ttctcgcgca ggagcagcga cgagcgcacc ttttcgccgt tcctgtcgtt
      181 gtgcgtggag agcaccaccc gcgcgcgcca tggaacaggc aactccgccg gggccggccc
      241 caggttcagg gcgaccagaa acctctcccg tgccgcttcg cgggcaaaag cgagcacgtc
      301 accggatgct ttcaccggct ggtaccgtcc gacggtaagc gcccggtgct cccggcgcag
      361 cgcaagcagg cgccgataga gggaaagcat ggaggcaggg ttcgtcgact cggcatcgac
      421 gttgatttcg tgcgccttcg gcgacagcgg cagccagggc gagccggacg tgaagccggc
      481 attcaagccc ctgctccact gcatcggagt gcgttgtgga tcgcggccga agccctgccc
      541 ggggacattg cgttcgaatg gatccatgac ttgcgactgg aggatctcca cgttgtccat
      601 gccgatctcg tcgccgtagt agatcgttgg cgtcccgcgc aaggtcagca acagcatggc
      661 ggcaacgcgc gcctgcgccg ggcccacgcg cgccacgatg cgcggcttgt catggttgct
      721 caacacccag ttgggccagc caccctccgg gatcgcggct tcgtactcct gtatctcgcg
      781 ctgcaagctg cgtgcgtgcc acgcagcctg gatcaggtgg aaattgaagg gaacgtgagc
      841 gcctccgaga tcccgcccgt agtacgcgac gagccgggcg agcggaagat atatctcgcc
      901 gatcagcagg cgatcatccc cgtattccgc gaggacgctt cgcatttccg acacgatctc
      961 gtgaacttca ggcctgtctg cggagtagag ctgcagcact ttcttgtgag gcggcatgcc
     1021 gggtgcccag tccggatttg gcgggttgtt gcgaaactgg tcgtccttga ccaggtgcca
     1081 cagcacgtcg acgcggaatc catccacgcc gcgttccagc cagtacctga ggacgtcata
     1141 catcttcgcg cggacggccg gattgcgcca gttcaggtcc ggctgctgcc gcaggtaggc
     1201 gtggtaatag tactgctccg ttcgttcgtc ccactcccac gcgctgccgc cgaagtcgct
     1261 gagccagttg ttcggtggtc cgcccccggg cgcgggatcg cgccagatgt acatgtcgcg
     1321 catcgggctg tctcgcgaac gccgcgcctc gatgaaccat gggtgctggt ccgaagtgtg
     1381 gttgggaacg aagtcgagca gcacttgtat ttttcttgca tgtgcggccg ccagtaacct
     1441 ttcgaaatcc tcgagcgttc cgaaaatact gtcgacaccc ctgtagtcag cgacgtcgta
     1501 cccgaaatcg gccatcggcg aggggaatat cggagagatc cagatcgcgt ctacaccgag
     1561 ccatgcgagg tagtcgagcc gcccggcaat gccgtgcagg tcgccgatcc cgtcgccctt
     1621 gctgtcctgg aatgagcgcg gatatatctg gtagatgacg ccggccttcc accactgatc
     1681 gtgctcgtgg tgcgtttgct gcaccacgat tggcacgtca gtcatagtgt cgccggccca
     1741 ttccataggc ggcaaaggcg aggcccgcga gcagggctag gccggcagcg gcagacacgc
     1801 cacgggcggt gccgagcgcg atgtcgccgc cggacgtgct cgactcttca tcgaaacggc
     1861 cgtggctgcc gaagtcgcct ggtgcaggtt cgaagaggtt gtccggccgc gtctgcgccg
     1921 gttccgaagc ctgctggccg cggtacgctt tcgccgcaag gatactgtcc accacgccgg
     1981 gagccaccat gctgccggcg acgagctgca ccgacgaacg gccgatgagc atctcgcgcg
     2041 gcgcggtttc ggcggcgcgc acgatcgacg acgcaatgac ttccggctgg taaaccggcg
     2101 gaaccgggcg gggccgattc ggcatcttgt tccgcgccca gtcgaactgc ggtgtgttga
     2161 ctgccggcag aaccacgagc gtcacgcgaa catcgctgcc ctcgtggagc agctcacatc
     2221 tgagcgactc ggtaaaaccg cgggcggcaa acttggcacc gcagtaggcc gcttgcagcg
     2281 gcagaccgtg gtaagccagc gccgaactga cgttgacgat cgtgccacgg ttgcgagaac
     2341 gcatccgtcg cagcgcactc agcgtaccgt atacctggcc caggtaagta acctcggtga
     2401 cgtgccggaa ttcctctgca gtgatctccg ccagcggcga gtagacagtt accatcgcag
     2461 agttgaccca ggcgtctatc gctccgaatt tttcttcggc tttttccgcc gccgcttcga
     2521 ccgcaccggc atctgcgaca tcagtgggca ctgccaacgc ctctccccct gcctttcgac
     2581 ctctgcgacc gccgcatcca ggcgtccggg ctcgcgtggt cgacaataat gcgaatgccg
     2641 aactcgcccg cggtgtgcag gcattcgagg aagtcctcga acagtccgta acgcgtgtcg
     2701 acgcgcaggt agtcggttat gtcgtagccg ttgtcgcggt tcgatgacgg gtagaacggc
     2761 actatccaga gacaggtgat gccgaggtcc gcaaggtaag ggagctttga cgtcaggcct
     2821 ttgaaatcgc cctggccatc gccgttggag tcacagaaac gttccacgtc gacggcgtag
     2881 atgatgacgt ctttccacca ggggtcggag attcgctcct ccggcgatgg tgtcctatcg
     2941 cccactgcta ctgctcctta gcgcaacaac cagtggctct ggctgcaaga tctatgccgt
     3001 gcatgccgca ttgggaggat gctggtacac ttcagccgcc cacgggagcc tccgacgggc
     3061 tcctgccagc caggcccggg tggcggaaca ggtagacgct acggacttaa aatccgttgg
     3121 ccgaaaggcc gtgccggttc gactccggcc ccgggcacca ggggcgacac ggtgaattga
     3181 ggcatacctg gcacggctca ggcctccgtc ccggaccctg ccctgccgtc ggcaaccgtc
     3241 agtcggctat gcaccacatg aatgcctttc accgacgcgg cgcagtcctg tgcgaattgc
     3301 agctgctctt ttgtcgggac cgtgccgaac aacgtcaggt ctccggttgc ctcatccagc
     3361 gctactccga tctgcgaagc attcacgcgc tgatctgtcg caagggcttc gcacaccagt
     3421 ttctccaggt tcggccgctt gaaatacttc gtgcctattc ctgatactgc tccttcttcc
     3481 agatcgctca tttcttctcc ttgttcgctt ttgctggcgc aagcgagaag agtctccaga
     3541 cacaggtagc aagattcgta ccacggggtt ttctatgtgg cctctcggca atacctgcgg
     3601 gcccgggggc tacggtgccg cgatgctgtc gccaaaacgt gacatctcac tgccgcggac
     3661 agcaaaattc tgcacgtcct gcagaatttg cactctccag gggagctatc tacaccgctt
     3721 cagggttttg caagcgttcg cgacgtgcgc tcgttcgcgt cgcgatcttt ttcgctacta
     3781 tgatttcctt ctctgccgac gaaacaggag ggactggcta tgaaacttgc ggatattctg
     3841 aagcagaacg caaacagaag catggatgtt gaaatggtga atggtgccac cgttactgcg
     3901 ggtagttggt caatcggtga tgagctggtc ggcgcgcaca aacagaaagg ttatacgatc
     3961 tacatttgcc tggataaaat cgtctccatc aaagtggaat cagcccccta gcgccgatgc
     4021 agacgccatc aaatggatgc cacgccgcct gagccggcgc caaagaccat caggatcagc
     4081 aaataaacca ggctaaccgc cccaccgcca cctgaatcat cgatacgtgg gcgtccgccg
     4141 gggaggtgat ccttcatcag ggggtggatc ctcttcggcc gcctgatcct cgccgctctc
     4201 gaagaccgac agttcgatcg cagcagtacc cgagaatccg tcgctatcgg tcaccgaggc
     4261 agtgatagaa tgtttaccca cgggaagcgt cgatgtcgaa aacgcaccgc ctctgccgac
     4321 caatcctgcc tggttcgaaa cccattcgag gctcgacgtc aggtcaccgt cttctgagtc
     4381 cgtggcgcta ccgacgaaat ggagggtgga gccttctggc tcgcttgcgc catctgcggg
     4441 cgaactgatc gtcacgaccg ggttctggcc cggatccggc gtgctgccgt catcatgcac
     4501 cgattgcgca atcggctcga gaaatggctc tatcactgag tagtcgctgt tccagccgga
     4561 ccgccagcga ttacccgcgc gggatacaac cacatccagg ctcgggatca cgatgataaa
     4621 gctgtcgcgg aggccccagg accagaaggc gtcggacgga acgttcgcca gcctgccgtc
     4681 gccgttgttc caccacagca gcccgtaatg gctggatgcg tccgggtggt cgtcttcctg
     4741 gtgcaccggc agaccctgca ggacggggtc gggacgtgcc gcctgttcaa cgaacgcccg
     4801 cggaagaatt tgttgccctt cccacgatcc catccgcagg tacaggtacc cgatacgcac
     4861 catggcatcg acatcagcat gaatgccgga gccgaactct cgacgcgtga tgccagcaat
     4921 agtgtcatcg cgataattgt gattgcgcca ggttatatcc ttgtcatcga ggccgagagg
     4981 ctgaaatacc cgggcaaaca tcaagtcttt cagatcgctg gcgaaccgaa ccgtcagcag
     5041 atcggcaagc cagttggaac cgccgtcgga gtacgcccac atgctgcccg gctcgaacaa
     5101 aaggtcaata tagcccccgg ttttgtcgaa ccctgcactg tgggtggcaa ggtgccggac
     5161 tgtgatgtcg tccagccagg cccttgcggt gttgctctcg ggcggcacac cgattcctgg
     5221 aaggtgcatt tgtgcggcat cgtccagctg aaccaaacca tcctgtaacg caaggccgag
     5281 agcggtaacc ccgatggact tcgttgtcga tttgagatcg taaagcgttt cgtggtttcc
     5341 ccaggaaaaa accagctttc caccgcggat gatcatcccg gagccgccgc cccgtagcgc
     5401 gtgatcccgc gcctgctcaa gcagcgcctc atccattccg gcttccacag gagtcgagcg
     5461 ctcccagtta gccgccggga agatcgcgag gaaaggcttc ggctcctccg cgttaactcc
     5521 cagggaacac aacagcagga ctgctataca ggcaattgcc gccggttctt tgccggcttc
     5581 gcttttccgg attaagcctt cgtgggtgcc cacttgcagt atttcgacct ccggtttccc
     5641 gtgaaactgc atatatggac ttctcagccg tagctggccc tgatggcgct gagatagccc
     5701 gccacgccgc catacgtgca tgtgatgcaa ttaatgcgcc actgccggag gttcggaagc
     5761 aactgcggca cgggatgcgg aaagggtgct ggcaaacacc tgcctccgcg ggttggtgag
     5821 cttggaatgc tctcttggag gctagggacg actcctaaca gccgcaccag taaagggctg
     5881 tagcaccaaa aaggcgtttc ttacccctga tactaccccg cccgcctgtc ccctagcggc
     5941 cgtctcggac tgtgtgcgca gccgggacgg cctcaccatc aatcgcactg gtaaggagta
     6001 cgacaatggc taggaaagag gataacaccg ctccggcgcc tgaggcccta gcggcatggg
     6061 acaagctcca caacgaacgc gagatggctg aacgcgtatc tcgacacttc caacgacgtg
     6121 agcttccacc agccggaggg aaaagtaccg gctgagactg cccgagaact ggcaacagca
     6181 gcgatcctgc tggagcaaac gaacacgctg cttgatcggg tcggcgaacg gaagccgccg
     6241 aggccggtcg acgacgaaaa ggcagcgtcg gacgccgcac cgctagacgt aagagacgag
     6301 aaaattccgc agctaatcgg cgacggcgat atgagcccgc agcgagtggc tgaccacctg
     6361 gcggcgatca aaagtcagaa gcccttacgc aaggctcaaa cttggctgtg ggatgcagag
     6421 caccttctgg acgttctgag cgtgtac
//
LOCUS       DASUOM010000289         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_855386,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000289 DASUOM010000000
VERSION     DASUOM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855386"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFY27_06115"
     CDS             <1..875
                     /locus_tag="VFY27_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5765124.1"
                     /translation="SAGEVLGFLGPNGAGKSTTMKMLTGFVAPDAGTASVCGHSITEN
                     PLAARRHLGYLPEGAPCYPEMTPGSFLSFIADIRGLAGETRKARLAEVTEQLHLGPVL
                     GQSIETLSKGFKRRVGLAQAILHDPDVLILDEPTDGLDPNQKHEVRELIRSMSADKLI
                     VISTHILEEVEAVCSRAIIIASGKILADDTPSNLAATSRLHNAVHITLAEEAAFDEVR
                     AAIEKLPSVAGTESDRTRRSITALARSGSEDAGTLRDISNLVSGNRWNVTSLQLEGGR
                     LDEVFRQVTQGSAA"
     gene            872..1606
                     /locus_tag="VFY27_06120"
     CDS             872..1606
                     /locus_tag="VFY27_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765125.1"
                     /translation="MSIISALFRRELRSYFATPVAYVFIVIFLVLMGTFTFYLGGFYE
                     RGQADLSAFFNYHPWLYLFLVPAIAMRLWAEERKTGSVELLMTLPVTPWQAVLGKYLA
                     AWAFTGIALALTFPIWITVNYLGDPDNGAIMAAYVGSFLMAGGFLAIGACLSATTKNQ
                     VIAFVLTVVACFGFLLSGFPLVLDLFSAWAPQTIVDGIASLSFLTHFSNISKGVIDFR
                     DLVYFALVIVVFLYANTIVLQWKQAD"
     gene            1608..>2835
                     /locus_tag="VFY27_06125"
     CDS             1608..>2835
                     /locus_tag="VFY27_06125"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX5765126.1"
                     /translation="MTTSSQKSLSTTSLLLLALAFIAAVIISNQLFRGWRIDLTDNQL
                     YTLSDGTKRILEKIDEPINLYFYFSDRATENIPSLRSYANRVRELLEEMEDEADGQIR
                     LNVIDPLPFSEEEDRAAQFRLQGVQIAASPDTIYMGLAGTNSVGDEEIIPFFQPDKEE
                     FLEYDIAKLISTLAEPERQAVGLISGVRMAGDFNPQTQQMQSPWVIFQQAQQLFDVRD
                     LGTNVETIDEEIGLLWIVQPKNLPDSTLYAIDQFIMGGGKAVIFVDPLAEADPSAAPQ
                     GMPAGMPPQGQASNLAKLFDAWGLSYTTSNVVADAGLALQISSGMTGRPVRHLGLLGV
                     TSAQLNSEEVVTADLEVLNLGVPGHISINDESALSFVPLVRSSQNAATIPASRFSFLP
                     DPAALQDDFAPTGEEYV"
BASE COUNT          548 a          907 c          830 g          550 t
ORIGIN      
        1 gttcagccgg ggaggtttta ggctttctcg gccccaacgg cgccggcaaa tcgacgacga
       61 tgaagatgct gaccgggttc gtcgcacccg atgcagggac cgcatccgtc tgcgggcaca
      121 gcatcaccga gaacccgctg gccgcacgca ggcacctggg ttacctgccg gaaggcgccc
      181 cctgctatcc cgagatgacg ccgggatcgt tcctgagctt cattgccgac atacgcggcc
      241 tcgccggcga gactcgaaaa gccaggcttg cggaggtgac ggaacagctg cacctcggcc
      301 cggtactcgg ccagtccata gagacgctgt ccaagggctt caagcgccgt gttggcctgg
      361 cccaggcgat cctgcacgat cccgacgtac tgatcctgga tgaaccgacg gacgggctcg
      421 acccgaacca gaaacacgag gttcgggaac tcattcgttc catgtcggcc gacaagctca
      481 tcgtgatatc gacgcacatc ctggaagagg tcgaggcggt atgcagccgc gcgatcatca
      541 tcgcctccgg aaaaatcctg gcagacgaca cgccctcaaa ccttgcggcg acgtcccgcc
      601 tgcacaatgc cgtgcacatt acccttgcgg aggaagcggc gtttgacgag gtccgggcgg
      661 cgatcgaaaa attgccctcg gtagccggca cggaatccga ccgcacccga cggtcgatca
      721 ccgccctcgc ccgcagcggc agtgaggacg cggggacatt gcgcgacatt tccaacctcg
      781 tatccggcaa ccgctggaac gtgacaagcc tccagctgga gggcggccgg cttgacgagg
      841 tgtttcgcca ggtcacgcag ggcagcgcgg catgagcatc atttcggcac tgttcaggcg
      901 cgagctcagg agttacttcg caaccccggt cgcctacgtg ttcatcgtca tcttcctcgt
      961 cctcatgggt acgttcacct tctatctcgg aggtttctac gaacgcggac aggccgacct
     1021 cagcgcgttc ttcaactacc acccctggct ctatcttttc ctcgtgccgg caatcgcaat
     1081 gcggctgtgg gcggaggagc ggaaaaccgg cagcgtagaa ttgctgatga cacttccggt
     1141 tacgccatgg caggccgtgc tcggcaaata ccttgctgcc tgggccttta ccgggattgc
     1201 cctggcgctg actttcccga tctggatcac cgtaaattac ctgggcgacc cggacaacgg
     1261 cgccatcatg gcggcctacg ttggcagctt cctcatggcc ggcggtttcc tcgcgattgg
     1321 cgcctgcctt tcggcgacga ccaagaacca ggtgatcgcc ttcgtgctta cggtggtcgc
     1381 ctgcttcggc ttcctgcttt caggcttccc gctcgtgctg gacctcttct cggcctgggc
     1441 gccccagacg atcgtggacg gcatcgcatc cttgagtttc ctgacccact tttccaacat
     1501 cagcaaaggc gtgatcgact ttcgcgatct cgtgtatttc gccctggtga tcgtggtgtt
     1561 cctgtacgcg aacacgatcg tcctgcaatg gaagcaggct gactgatatg accacctcct
     1621 cccagaaaag cttaagtacc accagccttt tgttgctggc gctcgcgttc atcgcggcag
     1681 tgatcatcag caaccagctg tttcgcggct ggcgcatcga tctgaccgac aaccagctct
     1741 acaccttgtc tgacggcacc aagcggatcc tcgaaaaaat tgacgagccg atcaacctgt
     1801 acttctactt ctccgaccgg gccacggaga acattccttc gctgcgaagt tacgccaacc
     1861 gcgtgcgtga gctgctggag gaaatggagg acgaggcaga cggacagatc cgcctgaatg
     1921 tcatcgatcc gctgccgttt tccgaggagg aagatcgtgc ggcgcagttc cgcctgcagg
     1981 gcgtgcagat cgccgcttcc ccggacacca tctacatggg ccttgccgga acgaacagcg
     2041 tgggcgacga ggagatcatc cccttcttcc agccggataa ggaggagttc ctcgaatacg
     2101 acatcgcgaa gctcatcagc acgcttgccg agccggagcg ccaggcggtg ggactgatat
     2161 ccggcgtccg catggcgggt gacttcaatc cgcagaccca gcagatgcaa tcgccgtggg
     2221 taatattcca gcaggcacag cagctgttcg acgtgcgcga cctcgggacc aatgtcgaga
     2281 cgatcgatga ggagatcggc ctcctctgga tcgtgcagcc gaaaaacctt cctgattcga
     2341 cgctgtacgc catcgaccag ttcatcatgg gcggcggcaa ggcggtcatt ttcgtggatc
     2401 cgctcgcaga ggcggacccg tcagccgcac cgcagggaat gccggcgggg atgccgccac
     2461 agggccaggc atcgaatctc gcgaagctgt tcgatgcgtg gggactctcc tataccacga
     2521 gcaatgtggt ggcagatgcc gggctggcgt tgcagatttc atccggaatg accggccgcc
     2581 cggtccgtca cctcggcctg ctcggcgtta ccagcgcaca gctcaacagc gaggaagtcg
     2641 tcaccgctga cctcgaagta ctgaacctcg gagtgccggg ccatatcagc atcaacgacg
     2701 agagcgccct ttcttttgtg ccacttgttc gctcgagcca gaatgcagcg acgatcccgg
     2761 cttcaaggtt cagcttcctg cccgacccgg ctgcgctcca ggatgatttc gcccccaccg
     2821 gcgaggaata tgtca
//
LOCUS       DASUOM010000290         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_940650,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000290 DASUOM010000000
VERSION     DASUOM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940650"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1518
                     /gene="pabB"
                     /locus_tag="VFY27_06130"
                     /pseudo
     CDS             82..1518
                     /gene="pabB"
                     /locus_tag="VFY27_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
     gene            complement(1570..1770)
                     /locus_tag="VFY27_06135"
     CDS             complement(1570..1770)
                     /locus_tag="VFY27_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX5765127.1"
                     /translation="MQRALIVVGILVLVIGLLWPWVSQLPFGRLPGDIVVSKPGFRFF
                     FPITTMILLSIVVSAILWFFRH"
     gene            complement(1786..3195)
                     /locus_tag="VFY27_06140"
     CDS             complement(1786..3195)
                     /locus_tag="VFY27_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5765128.1"
                     /translation="MNRDKTTEFVIRHWDESVIPTLSDYIRIPNKSPIFDPEWEQHGY
                     MERAVSLLEEWCRQQPIAGMKAEIVRLPGRTPLLFIDIPGAGDDVVLLYGHYDKQPEF
                     SGWIDGLEPWTPVLRDGKLYGRGGADDGYAVFGSLTAIRALQEQGVEHPRCVVIIEGC
                     EESGSFDLPHYIETLEDRIGSPGLVICLDAECGNYNQFWCTTSLRGNLVGTLHVDVLS
                     EGVHSGSASGIVPSSFRILRHLLSRVEDPSDGRILVPELHADIPGERRQQARLAAEVL
                     EDSVWQRFPWLDTESAPRETPLELILNNTWRPTLSVTGADGLPALVNAGNVLLPRTTL
                     KLSFRLPPTCEPRAASRALKATLEANPPPFVKVRFDVESTMGGWDAPPVADWLESSIK
                     EASETWFGQPSMYMGTGGSIPFMGMLGEKFPEAQFLVTGLLGPRSNAHGPNEFLHIET
                     GKRLTCCVSKVLEDYFQRT"
BASE COUNT          642 a          992 c         1052 g          614 t
ORIGIN      
        1 gtcacgcggg ctggaggtgt cttgcccctg gtcgtcctcg gactttttcg cgagcctgag
       61 cggcggcaat gcttgccgca catgaatgct gcgccacggt tgctccccga gtgggaattc
      121 acaggctccc gcgagcgcta cctttcgagc atcggcagaa tcaaggacga gatccggcgc
      181 ggcaacacgt accaggtcaa tcacaccgtc aggcaaaagg ccgaccacgt cgccgaccct
      241 tgggcgctct tcctggcaac ggcgatcgat gcgccgttcg ctgcgttcat cgacctcgca
      301 gggttttcga ttctgtccgc ctcaccggag ttgtttttcg acctgggcgg caaccggctg
      361 acatgcaggc ctatgaaagg cactgccgcc aggggcatga cgaccgctga agaccgtgac
      421 cgcaagcgca ggctggaagc ttccgtcaag gaccgcgcgg aaaacgtgat gattgcggac
      481 atggtgcgca acgacctcgg gagggtcgcg atacccggat ccgtcaaggc tgccgcactg
      541 tacgagatcg agaagtaccg gaccgtctgg cagatgacat cgacggttac ggcatccacc
      601 gatgcttccg tgctcgatat cttcgctgct ctgttcccgt cggcctccgt aacaggtgca
      661 cccaaagtgg cgagcatgaa gctcatcgcc gagctcgaag actcgccgcg ggaagtgtac
      721 accggcgcca tcggttacct ggcgcccgac cggcaggcca ggttcagcgt cgctattcgc
      781 accgcggtgg tcgataagga gacctccacc ggtcgctacg gagtgggagg cggcatcgtc
      841 tgggactcga acccggaaga ggaatacgag gagtgcctca acaaggcgaa ggtactcgcg
      901 tcggcggtgc ccgatcagga cttcaaattg ctcgaaacga tgcgctggga ccggaacgag
      961 ggctatgcgc tgctcgatga gcacatggag aggcttgaag cgtccgcaga gtatttcgat
     1021 tttgcactgg agcgtgctgc gttggaaagg cagctggaaa ccctggctgc cggcttcgca
     1081 ccggggcaga gtgttcgcat ccggctactg gtggcaaagg atggcgaatt gcaggtcgag
     1141 acgcgaccac tgccggcaga ggacaaaccg gtacgatggc gccttgccct ggcaaccttc
     1201 cctgtagaga cacgggatat tttcctctac cacaaaacta cccagcggga agtgtacgac
     1261 agggcgctcg ctgcaattgg cgactgcgac gacgtggtgc tgtggaaccg gacgggtcat
     1321 gtcaccgaga cgagcgtggg caacctcgtt gtccgtctgg aggggcagct ctggacgccg
     1381 ccggtgagtt gtggattgct ggcgggaacg tatcgccgca agctgttgcg ggagagtgtc
     1441 atcgcggaga ggccaatcac cctggaagaa gtcgggagcg ccgaagatat cttcgtaatc
     1501 aactccgtac gcggctggat cccctgcacg ttgcgaaggg agccaatcga tccgcctcgt
     1561 gacgcagtct cagtgtcgga agaaccacag gatcgcactg acgacgatgc tcaggaggat
     1621 catcgtcgtg atcgggaaaa agaagcgaaa gcctggcttg ctgacgacga tgtcaccggg
     1681 caggcgtccg aacggtaact ggcttaccca tggccagagc aggccgatga ccaggaccag
     1741 gattccgact acgatcagcg cgcgctgcat taaccgctcc ttgcttcagg ttctctggaa
     1801 gtaatcctcc aggaccttgg agacacagca ggtcaggcgt ttacccgtct cgatgtgcag
     1861 gaattcgttc gggccgtgag cgttagatct gggtccgagc aggccggtga ccaggaattg
     1921 ggcttcggga aatttctcgc ccagcatgcc catgaacgga atgctcccgc cggtgcccat
     1981 gtacatcgac ggctgcccga accacgtctc agaggcttcc ttgatcgatg actccagcca
     2041 gtcggcaacc ggcggcgcat cccagccgcc catggtcgac tccacatcga agcgcacttt
     2101 gacaaacggc ggcgggttcg cctcgagcgt ggccttgagc gcgcggcttg ccgcgcgggg
     2161 ctcgcaggtg ggcggcaggc gaaacgacag cttgagcgtc gttcgcggca acagcacgtt
     2221 gccggcattg acgagggcgg ggaggccgtc ggcacccgtg acgctcaagg tcgggcgcca
     2281 cgtattgttc aatatcagtt ccagcggtgt ttccctgggc gcggattcgg tgtcgagcca
     2341 gggaaagcgc tgccacaccg aatcctcgag aacctccgca gccaaccgtg cctgctgtct
     2401 gcgctccccg ggtatgtcgg cgtgcagttc cggcacgagt atgcggccgt cggacgggtc
     2461 ttccacccgg ctcagcaagt ggcgcaggat gcggaagctg gagggcacga tcccgctcgc
     2521 gcttccggag tggaccccct cgctcagcac gtctacgtgc agcgtgccga cgaggttgcc
     2581 gcgcaaggat gtggtgcacc agaactggtt gtagttgccg cattccgcat ccaggcagat
     2641 taccagtccg ggagatccta tccggtcctc cagagtctcg atgtagtgcg gaaggtcaaa
     2701 gctgccgctt tcctcgcaac cctcgatgat gaccacgcag cgcggatgtt caacgccctg
     2761 ttcctgcaag gcgcgtatgg cggtgagcga gccgaagaca gcgtagccgt cgtctgcgcc
     2821 cccccggccg tacagcttgc cgtctcgcag taccggcgtc cacggctcca gcccgtcgat
     2881 ccagccgctg aattcaggct gcttgtcgta gtgcccgtag agcaggacca catcgtcgcc
     2941 cgcgccggga atgtcgatga agagcaaggg tgttcgcccc gggagacgca cgatctctgc
     3001 cttcattccg gctatgggct gctgccggca ccactcttcg aggaggctca ccgcgcgttc
     3061 catgtatccg tgctgttccc actcgggatc gaagatcggg gacttgttcg gaatccggat
     3121 gtagtcggaa agcgtgggta tgaccgactc gtcccaatga cgaatgacga actcagttgt
     3181 cttatcccgg ttcatgtctc tgaccaattc atgtggatgc gttgcccgga gcttccatga
     3241 acagtccggg ccaggcagtg gttcaggcgc aaagcctgaa tcaattggcg agagaaggca
//
LOCUS       DASUOM010000291         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_677653,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000291 DASUOM010000000
VERSION     DASUOM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677653"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..>2526)
                     /locus_tag="VFY27_06145"
     CDS             complement(74..>2526)
                     /locus_tag="VFY27_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765129.1"
                     /translation="NERVKDARMQTVLQDLTFTLRTLRKNPGFAVVAILTLALGIGAN
                     IAIFSVVDHVLLRPLNLADPDRIVRVLNNAPEDGYATSRGAFSPQDFEDLERDSTAYE
                     RLAAFRFVPGNSSSTLLGEGAPEEIETAHVSAEFFATFGVSPKLGRVPTAHENVDGAN
                     RVAVLSESFWQQRFGGDTGIVGRSVSLDGEPFTVIGVMPQSFEFPSRAAKAWLPISLI
                     GEDDIPHRRGLRWMEAVGRLAPGVTLEAADAQTNSILQRLESEYPDSNNGWGSATVQT
                     LHESLVGDVRPALLLLLGAVGLLLLISCANLANLLLVRGSVRGRELAIRTALGADRGR
                     VVQQLLTESVALALCGGAVGVGVAYVGLEALLALSSGTIPMPEQIALDARVMAFALAA
                     SLLTGVVFGLVPSLSASRPNVNDALNDGGRGSTEGLRGHGVRNLLVVAQTALAVVLLA
                     GAGLLIRSFWNLTHVDPGFTGENVLTVSIDTPSEVLGSERRNQYRQEILSRLRNIPGV
                     VAVGGSKTVPLHGGGEPYAFTIPGGPDAKPVTPESGVLIVTPGYFEALGIPMLQGRGF
                     TTADENSGANVLVVNEALARRYLPGKNPVGENLKLGEFDFHIVGLAGDVRNDGMARGA
                     EPAVYAPASLLPRSSMKLFIRTSSDPLQVAADVRRTIWQVNPNQPVSDIATMQQVVSK
                     TVSRPRFFTVLLGVFAGLAVVLAALGVYGVIAWSVSRRAYEIGIRMALGAGAGRVLRM
                     VILQGIAPTLAGLALGLAAALALTRLLSSLLFEVGAADPATFAGVAALLLAVGLLASW
                     LPAWKAARVNPMSALRSE"
BASE COUNT          503 a          864 c          748 g          411 t
ORIGIN      
        1 ccgcagcatg cgcagggcaa aacgaacttc ccgtatcagc tcgtccacga ttttcgcctg
       61 tgtccccaac cggtcattcg cttctcaacg cggacatcgg attgactcgg gcggccttcc
      121 atgcaggcag ccagcttgcc agcaacccga ccgcgagcag caaggcagcc acgccggcaa
      181 aggtcgcggg atccgcggcg ccgacctcga agagcaggct tgatagcaag cgcgtcagtg
      241 ccagggccgc ggccagtccc agggcaagcc cggcgagcgt cggcgcaatg ccctggagga
      301 tgaccatccg cagcaccctt cccgcacccg cacccagcgc catgcggatg ccgatctcgt
      361 acgcgcggcg gctaaccgac caggcgatca cgccgtaaac gccgagcgca gccagcacga
      421 cggcgaggcc ggcaaaaaca cccagcagca ccgtgaagaa gcgcgggcgc gaaacggtct
      481 tcgataccac ctgttgcatc gtggcgatgt cagaaaccgg ctggttcgga ttcacctgcc
      541 agatggtgcg cctgacgtcg gcagcaacct gcagcggatc cgaagatgtg cgtatgaaaa
      601 gcttcatgct ggagcgcggc agcaacgacg ccggtgcata aacggcgggc tcggccccgc
      661 gtgccattcc gtcattcctc acatcgcctg cgagccctac gatgtggaag tcgaactctc
      721 caagcttcag attttcgccc accgggtttt tgcccggcag ataacgccgc gccagcgctt
      781 cgttcacgac caggacgttc gcaccgctgt tttcgtccgc agtcgtgaac ccgcgcccct
      841 gcagcatggg gatgccgagc gcctcgaagt agcccggcgt cacgatcagg acgccggatt
      901 ccggtgtcac cggttttgcg tccggaccgc cgggaatcgt gaatgcatac ggttctccgc
      961 cgccgtggag tggcacggtc ttgctgccgc cgactgcgac gactcccgga atattacgca
     1021 agcggctcag gatttcctgc cggtattggt tgcggcgctc gctaccgaga acctcggacg
     1081 gcgtgtcgat gctcaccgtc aacacgtttt ccccggtaaa ccccggatcg acgtgcgtca
     1141 ggttccagaa gctccggatc agcagccctg cgccggccag cagcacgacg gccagcgccg
     1201 tctgcgccac gacaagcagg ttgcgcacgc cgtgaccccg cagcccttcg gtggaaccgc
     1261 gtcctccatc gttcagcgca tcattcacat tcggccggga agccgaaagc gacggcacca
     1321 gtccgaacac cacgcctgtc agcagcgatg ccgcaagcgc aaaggccatg acgcgagcat
     1381 cgagcgcgat ctgttccggc atcgggatgg tccctgaact gagcgccagc agggcttcca
     1441 gtccgacgta cgccacgccg acaccaacag caccgccgca cagggccagt gccacactct
     1501 ccgtcagcaa ctgctgcaca acccggcccc ggtcggcgcc aagcgcggtc cgtatcgcaa
     1561 gctcacggcc ccgcacgctt ccgcgcacga gcaacaggtt ggcgagattg gcgcacgaga
     1621 tcagtaacaa cagcccgacc gcgcccagca gcaggagtaa cgccgggcgg acatctccca
     1681 ccagcgactc gtgcagcgtc tgcacggttg cagaacccca gccgttgttc gaatcggggt
     1741 actccgactc gagtctctgc aagatggaat tcgtctgggc gtccgccgct tcgagggtca
     1801 cgcccggagc cagccgtcca accgcttcca tccaccgcag cccgcgtcga tgagggatgt
     1861 catcctcgcc gatcaacgag atgggcagcc aggctttcgc cgcgcgcgaa ggaaactcga
     1921 acgattgcgg catgacgcca atgaccgtga atggctcacc gtcgagcgaa accgatcggc
     1981 cgacaatgcc ggtatcgcct ccgaaccgct gttgccagaa gctctcgctc agcactgcaa
     2041 cacggtttgc tccgtcgacg ttctcgtgcg ccgtcggaac gcgcccgagc ttcggagaca
     2101 caccgaaagt cgcgaagaac tccgccgaaa cgtgcgctgt ttctatttcc tccggtgcac
     2161 cctcacccag caacgtgctg ctactgttgc cggggacgaa ccggaaggcg gcgagacgtt
     2221 cgtaggcagt actgtcgcgc tcgagatcct cgaagtcctg cggagaaaaa gctccgcgtg
     2281 acgtggcata accatcttcg ggcgcgttat tgagtacgcg cacgatcctg tcgggatccg
     2341 caaggttcaa cggccggagg agcacatgat cgacgacact gaagatcgct atgttcgcgc
     2401 cgatgccgag ggccagcgtc agtatcgcaa ctacggcaaa tcccgggttc ttccgcaagg
     2461 tgcggagcgt aaacgtaaga tcttgcagaa ccgtttgcat gcgcgcgtcc tttacccgct
     2521 cattgg
//
LOCUS       DASUOM010000292         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_758396,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000292 DASUOM010000000
VERSION     DASUOM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758396"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VFY27_06150"
     CDS             <1..1373
                     /locus_tag="VFY27_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5765130.1"
                     /translation="PAASQPPETAPHEPPAPEADVPPVGPSGESAVLADAGDAPEPGP
                     ETSGSDEEQAAVSGELEQGSSGPVQVDNRLTAGLAIDTMGGDLDESAPVVPLADAVTV
                     AEEKEAAHNLKLLDGEVPPGTYQRLSWAATELFEGIPVSTPVLAVNGKLPGPTLCLTA
                     AVHGDELNGIEMVRRVLHDLDPQKLSGAVIGVPIVNLQGFRRGSRYLPDRRDLNRYFP
                     GNPNGSAASRIAYSFFTEVVRHCDALIDLHTGSFERANLPQLRADLRNPDVVTLTHGF
                     GATVILHSDPAQGTLRYAASRIGIPTVTLEAGGPSELELKEVMHGVKGIDTLLNTLGM
                     VKKSRLWGDPEPIYYRSVWVRADRGGILLADVGLGSTVRRGDLLGTITDPMNNASTNL
                     YAPFSGRIIGMARNQVVMPGFAAFHVGIQTAEASAADAGSEADDAALLHDSEVTNDDY
                     IDGMSE"
     gene            1447..2382
                     /gene="lpxL"
                     /locus_tag="VFY27_06155"
     CDS             1447..2382
                     /gene="lpxL"
                     /locus_tag="VFY27_06155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEX5765131.1"
                     /translation="MSERKPLSHFRAPRYWPTWLGLGLLRLVCLLPYRMQLALGKLLG
                     RIAHRLAKERRAISRRNIELCFPELSPAGRHALALAHFESLGMSIIELGLGRWASDER
                     LLAMTRIEGAEHIRKATDRGIGVILLSAHFTTLEISGRVLSLNSPPFDAVYRKNRSGF
                     LTEILRTNREKSARGTIEKNDIKSMVRSLREGTPVWYAPDQSYNRKQSALLPFFGVPS
                     MTNTATGTLARLGRAVVVPFFPRRLPEGGYVLTIMPPIEGIPSADPIEDTRKYLRILE
                     DQIRLCPEQYYWVHRKFKNRPEPLADPYADLAALE"
BASE COUNT          478 a          777 c          751 g          419 t
ORIGIN      
        1 aaccggctgc ctcgcagccg cccgaaactg caccgcacga accaccggcc ccggaagcgg
       61 acgttcctcc agttgggccc tccggtgaat ccgccgtgct tgcagacgct ggtgacgcgc
      121 cagagccagg cccggaaacg agcggcagcg atgaagaaca agccgctgtc agcggggaac
      181 tggagcaggg atcgtccggt cctgtccagg tggacaacag gcttacggcc ggtttggcta
      241 tcgacacgat gggcggtgat ctggatgaat cggcacctgt cgtcccgctt gcggatgccg
      301 tgacggtagc cgaggagaaa gaagccgcac acaacctcaa gctattggac ggggaggtgc
      361 cgccgggtac ctaccagcgc ctttcctggg cggccacgga actgttcgaa gggattcccg
      421 tgtccacgcc ggtactggca gtaaacggca aactgcccgg gccgacgctt tgcctgacgg
      481 cggcggtgca cggcgacgag ctgaacggga tcgagatggt gcgccgcgtg ctgcacgatc
      541 tcgacccgca aaagctctcc ggtgcggtga ttggagtacc tatcgtgaac ctgcagggtt
      601 ttcgccgcgg gtcccggtac cttcccgacc ggcgtgacct gaatcgttat ttccccggca
      661 acccgaacgg cagcgccgca tccaggatcg cctactcctt ctttacggaa gttgtccgtc
      721 attgcgatgc actgatcgac ctgcacaccg gttcgttcga acgggccaac cttccgcaac
      781 tgcgcgccga cctgcggaac ccggatgtcg tcaccctgac ccacggcttc ggcgcgaccg
      841 tcatcctgca cagcgatccg gcccagggaa ccttgcgcta cgccgcgtcg agaatcggca
      901 tccccaccgt cacgctggag gcaggcgggc cgtccgaact cgaactcaag gaagtaatgc
      961 acggcgtaaa gggaatcgat acgctcctga acacgctggg catggtgaag aagtcgcgtc
     1021 tgtggggaga ccctgaaccc atttattacc ggtcggtgtg ggtacgcgcc gaccgcggcg
     1081 gaatcctgct cgcggatgtc ggtctcggca gcactgtccg gcgtggcgac ctgctgggca
     1141 cgattaccga ccccatgaat aacgccagca ccaaccttta cgctcccttc tccgggcgca
     1201 tcatcggcat ggcccgcaac caggtcgtga tgccgggctt cgccgccttt cacgtcggca
     1261 tccagacggc ggaggcaagc gcggccgatg caggcagtga ggcggacgac gccgcgctcc
     1321 tgcacgactc cgaggtgacg aacgacgact acatagacgg gatgtccgag tagcccggca
     1381 cgtatccgtc gtacccggcg tacaatcggc tggcccggac ggcgcgctca aattaaagtg
     1441 ccgggaatga gcgaaagaaa acccctctcg catttccgcg cgcccaggta ctggccgacg
     1501 tggctcggtc tcgggctgct tcgccttgtt tgcctgctgc cgtatcgcat gcaactcgct
     1561 ttgggcaagt tgctggggcg catcgcgcac cggcttgcga aggagcgccg ggcgatcagc
     1621 cgccggaaca tagaactctg cttcccggaa ctgtcgccgg ccgggcgcca cgcacttgct
     1681 cttgcgcatt tcgaatcgtt gggaatgtcc atcatcgaac tggggctggg ccgctgggca
     1741 tcagacgaaa gactcctcgc gatgactcgc atagaagggg ccgaacacat tcgcaaggcc
     1801 accgaccgcg gcatcggcgt tattctgctc agcgctcact tcacgacgct ggaaatcagc
     1861 ggccgcgtgc tgagcctcaa cagcccgcca ttcgatgccg tgtatcgcaa aaaccgcagc
     1921 ggattcctga cagagattct ccgcaccaac cgcgagaaat cggcccgcgg cacgattgaa
     1981 aagaacgaca tcaagagcat ggtgcggagc ctgcgagagg gcaccccggt gtggtatgcg
     2041 ccggaccaga gttacaaccg caagcaatcc gccctgctgc cattctttgg tgtcccatcg
     2101 atgaccaaca cggcgacggg gacgctcgcg aggctgggca gggcggtcgt cgttcccttc
     2161 ttcccgaggc gcctgccgga gggcggttac gtccttacga tcatgccgcc gatcgaaggt
     2221 attcccagcg cagacccgat cgaagacacc aggaaatacc tgaggattct ggaagaccag
     2281 atacgtcttt gcccggagca gtattactgg gtacaccgca agttcaagaa caggcccgag
     2341 ccattggccg acccctatgc ggatctggcg gcgctcgagt agccgtgaat gagctgttgc
     2401 cagttttgct cggagaaagc gatac
//
LOCUS       DASUOM010000293         6677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_731201,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000293 DASUOM010000000
VERSION     DASUOM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731201"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..483
                     /locus_tag="VFY27_06160"
     CDS             121..483
                     /locus_tag="VFY27_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765132.1"
                     /translation="MADTARCYRCGESLAALTLPFSRRDECPSCAAHVHVCRMCVHFD
                     AVVPKKCREDDAEEVTEKERANFCDWFKPGSDTFDPVRASKAAKAESALAALFGGDEG
                     GEPRDADDQLKRAEDLFR"
     gene            499..1161
                     /gene="queC"
                     /locus_tag="VFY27_06165"
     CDS             499..1161
                     /gene="queC"
                     /locus_tag="VFY27_06165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5765133.1"
                     /translation="MKQKALVILSGGQDSTTCVYWAIERFGKESVESLTFDYGQLHRH
                     ELQAASRVAAYAGIPNVVLPINTFEAIGGSALTDPSIEVPRTTETGSLPATFVPGRNL
                     VFLTFAAAYAWRCDIQDLVIGVAQTDYSGYPDCRRETIDALEKTLALGLERRFTVHTP
                     LMNLSKKETVLLAKELGAMDAMALTHTCYNGSVPPCGECAACLLRAKGFAEAGIPDPL
                     IA"
     gene            1163..1813
                     /gene="queE"
                     /locus_tag="VFY27_06170"
     CDS             1163..1813
                     /gene="queE"
                     /locus_tag="VFY27_06170"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX5765134.1"
                     /translation="MTYSVKEIYYTLQGEGAQTGRPAVFLRFAGCNLWSGRERDRSTA
                     VCRFCDTQFVGTDGPGGGKFKNAAALAKAVAAHWRPSTTANGSAPYVVCTGGEPLLQL
                     DDAAIASLHERGFEVGVETNGTQRAPEALDWICVSPKAGAEVLQRHGDELKLVYPQVE
                     AEAQPERFAELDFTHHFLQPLDDSRIRENTRAAIEYCLRHPQWKLSLQTHKIIGMP"
     gene            1848..2285
                     /locus_tag="VFY27_06175"
     CDS             1848..2285
                     /locus_tag="VFY27_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765135.1"
                     /translation="MEQRDVLPTLAHKVVRFTLALCIGLALAFYAYHRVTDPEPARQR
                     ASEEAAVLAARDILRSYLEPAGEAEIVDPVSPRRKIGKSYIYPADAGWEISGHYRRSE
                     SDRWHPFLMAIDHDHRLLRLSVRDTPGNFTPAALQDRKLEVRQ"
     gene            2594..3778
                     /locus_tag="VFY27_06180"
     CDS             2594..3778
                     /locus_tag="VFY27_06180"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5765136.1"
                     /translation="MLALVWLAVPLVTRWANATVTVPMERLRIATVTRGDLVRDVSVQ
                     GRVVAAVSPTLYAPAAGSITLEVEAGADVKQGQVLAVIDSPALANELQQAQSALQQQT
                     VELERQRIESRQLALEKRKAADLADVTLVAAKREKKRADDANVLSVISAIDFEKAQDD
                     LRNAELAYQHAVADADLFDERLAFELRASELGVERQRLLVADLKRQVDALAIRSPVDG
                     IVGDLLVAQKAAVAKDTPVMAVVDLTRFEIDAQIPESYADDLALGMAAEILVGSERYP
                     GRLVAVSPEIVETLVSSRIRFEGDAPANLRQNQRLTTRVLLEERPGVLMLQRGQFLES
                     GAGRVAYVLEEGKLARRRLITIGARSLSAVQVESGLEEGDAVVISSLEPFQGAETVLI
                     TD"
     gene            3795..4490
                     /locus_tag="VFY27_06185"
     CDS             3795..4490
                     /locus_tag="VFY27_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765137.1"
                     /translation="MLCMHNISKVYRTDSVETHALREFSLTVEEGEFVSVTGPSGSGK
                     TTFLNIAGLLEEFSNGKYELDGKDVSRLNDRERSRVRNEKIGFIFQSFNLIPELDIYD
                     NVDVPLRYRGMKAAERKQRIERVIEMVGLASRMHHLPSQLSGGQQQRVAIARALAGDP
                     RFLLADEPTGNLDSAMAGSVMELLTEINNSGTTIIMVTHEATLARKARRNISVRDGRV
                     NSGQAIHIAAATA"
     gene            4504..5829
                     /locus_tag="VFY27_06190"
     CDS             4504..5829
                     /locus_tag="VFY27_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765138.1"
                     /translation="MIRYYIKLGVLSIRKNPVLSALMVAAIAVGISACMTILNINYVM
                     SGNPIPHRSEVLFHVQLDSWDPNQSADDGGLSEPPNQVTYLDGTALLKARKARRQVLS
                     YQSSRVVQPADRDLRPFEVNARSTTADFFPMFDAPFLYGRGWDQAADDSQEQVVVLGK
                     EVNERMFGGENSVGRTVMMNDDVYRVAGVLDDWPLVPRFYDVTTQAFNEVEELFFPFS
                     VSVAREVGGNGNTNCWKPWDDAGWNAFLNSECVWMQMWVELHGPDEKQDYLAFLDAYV
                     ESQKAIGRFPRPLNNRLNNVMEWMADQEVVADDVQVLLGLAILFLIVCLLNTIGLLLA
                     KIMRRSGDISLRRALGASRNAIFSQYIIEAGVIGLCGGILGIGLTWLGLRGIEVLYRQ
                     YEFVENLVKMDWVMVLAAVALAIISALGAALYPTWRACRIHPAAQLKTL"
     gene            5839..>6677
                     /locus_tag="VFY27_06195"
     CDS             5839..>6677
                     /locus_tag="VFY27_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765139.1"
                     /translation="MEIGPIWRALMRNKTAYVLIALQIAVTMAIMVNAVAIVQERHRL
                     MARPSGIDEPNIFHLESVSFTADFDQAAAIREDLDAIRSMPGVVDAIATNSVPLRNGG
                     WSSGIATEPGDDIEGMGVAIYFTDEHGIDTYGLELVAGENFAATDIGWYGLSSTRWPS
                     SGIISREAAATLFPDTPLNEVVGKTIYINSDDDVRIVGIVERLQAAWKSWDGVERSML
                     VPAFLEGSGTRYAIRTEPGYRDELMPRVEQRLAEMNDGRIVRDMMTMDATRDRAYHED
                     AAM"
BASE COUNT         1361 a         2001 c         2139 g         1176 t
ORIGIN      
        1 gcttgccttg cagacggcga aggtgggcgc gttcgatgga ccggggcgaa ctcgcccgcg
       61 tggcgggctc ttgcggcggc cggcggtata cttagccgtt tcaactcaag gggaagacac
      121 atggccgata ctgcgcgctg ctatcgctgc ggcgaatcgc ttgccgcgct gacgctgccg
      181 ttttcgcggc gggacgaatg tccttcctgc gcggcgcacg ttcacgtttg ccgcatgtgc
      241 gttcatttcg atgcagttgt gccgaaaaaa tgccgcgagg acgacgcgga ggaagtgact
      301 gaaaaagagc gagcgaattt ctgcgactgg ttcaagcccg gcagcgatac gttcgatccg
      361 gtccgcgcca gcaaggcagc gaaggcggag tcagccctcg ccgcattgtt cggcggcgac
      421 gagggcggcg agccgcgaga cgcggatgat cagttgaagc gggcggagga cctgttcagg
      481 taacactgcg ggcgtttcat gaaacaaaaa gctcttgtca tcttgtcagg aggccaggac
      541 tccacgacct gtgtctactg ggccatcgaa cgcttcggca aggagtcagt cgaatcgctt
      601 acgtttgact acggccaact ccatcggcac gagctgcagg ccgcgagtcg ggtcgcggcc
      661 tacgccggca tcccgaacgt cgtcctgccg atcaacacgt tcgaagcgat cggcggcagt
      721 gcgctgaccg acccgtcgat cgaggtcccg cgaacgaccg aaaccggttc gctgcccgca
      781 actttcgtcc cggggcgaaa cctcgttttc ctgacgtttg cagcggcata tgcgtggcga
      841 tgcgacatcc aggacctcgt catcggcgtg gcgcagacgg actacagcgg ttatccggac
      901 tgccgtcgcg aaacgatcga cgcccttgaa aagacgctgg cgcttgggct ggagcgccgc
      961 ttcacggttc acacgccgct catgaacctg tcgaagaagg aaacggtgtt actggcgaag
     1021 gaactggggg caatggacgc aatggcgctg acacacacct gttacaacgg gagcgtgccg
     1081 ccgtgcggtg aatgcgcagc ctgcctgctt cgcgccaaag gcttcgcgga agccggtatc
     1141 cccgacccgc tgatcgcttg acatgactta ctccgtgaag gaaatctact acacgctgca
     1201 gggcgagggt gcgcagacgg gccggccggc cgtgttcctg cgcttcgccg gctgcaacct
     1261 gtggtccggg cgcgaacgcg atcggtcgac cgccgtctgc aggttctgcg acacgcagtt
     1321 cgtcggcacc gacggccctg gcggcggcaa attcaagaac gccgccgcgc tcgccaaagc
     1381 ggtcgcggct cactggcggc cgtcgacgac ggcgaacggt agcgctccat acgttgtctg
     1441 caccggcggg gagcccctct tgcagctcga cgatgccgcg atcgcctcgt tgcacgagag
     1501 gggctttgaa gtcggcgtgg aaacaaacgg gacccagcgg gcacccgagg cgctggactg
     1561 gatctgcgtc agccccaaag cgggtgcgga ggtcctgcag cggcacggag acgaactgaa
     1621 actcgtgtac ccgcaggtgg aagcggaagc acaaccggag cgctttgccg agctcgattt
     1681 cactcatcat ttcctgcaac cgctggacga cagccgtatc agggaaaaca cgcgcgccgc
     1741 catcgaatac tgtctgcggc atccccagtg gaagctcagc ctgcaaacac acaagatcat
     1801 aggcatgccc tgaacgcata actgagcgcg gacccgattt gatggcgatg gaacagcgag
     1861 acgtcttacc gacactggcg cacaaggtag tgcgattcac gctcgccctc tgcatcggtc
     1921 tcgccctcgc gttctacgcc tatcaccgcg tgaccgaccc ggaacccgcc aggcaacgcg
     1981 ccagcgaaga ggctgcagta ctggccgcgc gagatatcct gcggagctac ctcgaacccg
     2041 ccggcgaagc cgaaatcgtc gaccccgttt cgccacggcg caagatcggc aagtcgtaca
     2101 tctatccggc ggacgccggc tgggaaatct cgggtcacta ccgccgcagc gaatccgacc
     2161 gctggcatcc cttcctgatg gctatagacc atgaccaccg gctgctcaga ctctccgtga
     2221 gggacacgcc cggcaatttc actcccgcag ccctgcagga ccgcaaactg gaagtgcgac
     2281 agtaaccggc tcgcagacgc aggtcatccc tgaccgggcc tgatggaggc taccgcgacg
     2341 actggggggc gaggccgaac cgcgcaattc ccattcgcta ccactgaaga cgcatccgct
     2401 taaacgcaca gcggagaaaa taagccagcc ccgatgccaa cgttccaccg cgctaccgca
     2461 tctaagccct gaatcctcaa aaattcataa gggcaagccg ctgaaaatcg ccgacacctc
     2521 cgggcaggac gtccgcctgg ctccgaagag caagcgcaac cgtcgcatcg taggaggcgg
     2581 catcgtcctg ctcatgctgg cactcgtgtg gctggcagtc cccctcgtaa cgcggtgggc
     2641 gaacgccacg gtgacggtgc cgatggaacg cctgagaatc gcgacggtga cccgcggcga
     2701 cctcgtgcgg gatgtttctg tgcaaggccg cgtggtcgcc gcggtaagcc cgacgctgta
     2761 tgcgccggcg gcgggttcca tcactctcga ggtcgaggcc ggcgccgatg tgaaacaggg
     2821 gcaggtactg gccgtcatcg acagcccggc gctggccaac gaacttcagc aggcccagtc
     2881 ggcgctgcaa cagcagacgg tggaactgga acggcagcgt atcgagtcgc gccagctggc
     2941 gctggagaag cgcaaggcag cggaccttgc ggatgtaacg ctggtggccg cgaaacgcga
     3001 gaagaagcgg gccgatgatg cgaacgttct gtcggtcata tcggcaatcg atttcgagaa
     3061 agcacaggat gatctacgta acgcggagct tgcgtaccag cacgcggttg cggacgccga
     3121 tctcttcgat gagaggctcg cgttcgaact gcgagccagc gaactgggcg tggagcgcca
     3181 acggctgctg gtagcagatc tcaagcgcca ggtagacgcg ctggcgattc gctcaccggt
     3241 ggatggcatc gtcggcgatc tgctggtggc gcaaaaagcg gcagtggcga aggacacacc
     3301 cgtcatggcg gtagtggacc tgacgcgctt cgagatcgat gcgcagattc cggagagtta
     3361 cgccgacgac ctggcattag ggatggccgc ggagatcctg gtcggaagtg agcgttaccc
     3421 gggtcggctg gtggctgtgt cacccgagat cgtcgaaacc ttagtgagca gccggatccg
     3481 cttcgaaggg gatgccccgg cgaatctccg gcagaaccag cgtttgacga cccgtgtcct
     3541 gctggaggaa cggccaggcg tcctgatgtt acagcgcggg cagttcctcg aaagcggcgc
     3601 aggccgggtc gcgtacgtgc tggaggaagg aaagctcgca cgccggcgcc tgatcacgat
     3661 cggcgctcgc agcctgtccg cggtacaggt cgaaagcggc ctcgaagaag gagacgccgt
     3721 cgtaatttcc agcctcgaac cgttccaggg cgccgaaacc gtcctcatta ccgattaacc
     3781 tcaggagacc agccatgctt tgcatgcaca acatatcgaa ggtgtacagg acggattcgg
     3841 tggagactca cgcgctgcgg gagttttcgc tgacggtgga ggagggcgag ttcgtctccg
     3901 ttaccggacc gtcgggctcg ggcaagacca cgttcctcaa tatcgccggc ttgctggaag
     3961 aattttcgaa cggcaagtac gaactcgacg gcaaggacgt atcaagattg aatgaccgcg
     4021 agcgctcccg cgtgcggaac gagaagatcg gcttcatctt tcagagtttc aacctcattc
     4081 cggaacttga tatctacgac aacgtcgacg tcccgttgcg ctacagggga atgaaggcgg
     4141 ccgaacgcaa gcagcggatc gaacgggtca tcgagatggt cggtctcgcc tcacggatgc
     4201 accacttgcc ttcgcaactc tccggaggac agcagcagcg tgtcgcgatt gcgagggcgc
     4261 tggcgggaga tccgcgattc ctgttggcgg acgagccgac cggcaacctg gattcagcca
     4321 tggcgggcag cgtgatggag ctgctgaccg agatcaacaa ctccggcacg accatcatca
     4381 tggtgacgca cgaagcgacc ctcgcgagga aagcgcgccg caacatttcg gtgagggacg
     4441 ggagagtcaa cagcggtcag gccatccata tcgccgcggc gacggcctag gcgggagcag
     4501 tcgatgattc gctattacat caagcttggc gtgttgagca tccggaaaaa cccggtgctc
     4561 tccgcgctca tggtggccgc cattgccgtc ggcataagcg cctgcatgac gattctcaac
     4621 atcaactacg tcatgagcgg caaccccata cctcatcgct ccgaggtcct ttttcacgtg
     4681 cagctggaca gctgggaccc gaaccagtca gcggatgacg gtgggctgtc ggagccgccg
     4741 aaccaggtta cctacctcga cggtaccgcc ttgttgaaag cacgcaaggc acgccggcaa
     4801 gtcctgagct accagtcctc aagggtcgtg cagccggcgg atcgcgacct gcggccgttc
     4861 gaggtcaacg cgcgcagcac cacggccgat ttcttcccga tgttcgacgc gccgttcctg
     4921 tacggcaggg gctgggacca ggcggctgac gactcgcagg aacaagtcgt ggtgctcggc
     4981 aaggaagtca acgaacgcat gttcggcggc gaaaattcgg tgggccggac cgtgatgatg
     5041 aacgacgatg tctaccgggt cgcaggcgtc ctggacgatt ggccgctcgt accgcggttt
     5101 tacgacgtca ccacccaggc ctttaacgaa gtggaagaac tgttttttcc tttcagcgtg
     5161 agcgttgcca gggaagtcgg gggcaatggc aacaccaact gctggaagcc ctgggatgat
     5221 gccgggtgga atgcgttcct gaactccgaa tgcgtatgga tgcagatgtg ggtcgagctg
     5281 catgggccag acgaaaagca ggactacctg gctttccttg atgcctatgt cgaatcgcaa
     5341 aaagccatcg gccgattccc gcgcccgctc aacaatcgcc tgaacaatgt catggaatgg
     5401 atggccgacc aggaagtcgt ggcggacgac gtgcaggtcc tcctgggact tgcaatactg
     5461 ttcctgatcg tgtgcctgct gaacaccata ggcctgttgc tggcgaagat catgaggcgc
     5521 tcgggggata tcagcctcag gcgtgctctc ggtgcctcgc gcaacgcaat tttttcccag
     5581 tacatcatcg aggcgggagt gattggattg tgtggcggga tcctcgggat cggactcacg
     5641 tggctcggcc tgcggggaat cgaggtgctc taccggcaat acgagttcgt cgagaacctg
     5701 gtgaagatgg actgggtcat ggttctcgca gcagtcgctc ttgcaattat ctcggcgctg
     5761 ggtgctgcgc tttacccaac ctggcgtgcg tgcaggattc accccgcggc acagttgaag
     5821 accttatagg aaacgaccat ggaaatcgga ccgatctggc gcgcgctgat gcgcaacaag
     5881 actgcctacg ttctcatagc cctgcaaatt gccgtgacca tggcgatcat ggtcaatgcc
     5941 gttgcgatcg tccaggagcg tcaccggctg atggcccgcc cgagcggtat cgatgaaccg
     6001 aacattttcc atctcgagag cgtcagcttc accgcggact tcgaccaggc cgcggctatc
     6061 cgtgaggatc tcgatgccat tcgcagcatg ccgggcgtgg tggacgccat cgctaccaac
     6121 tctgtgccgt tgcgcaacgg cggctggtcg tccggcattg cgacggagcc gggggatgac
     6181 atcgagggta tgggggtagc gatctacttc actgatgagc atggcatcga cacgtacggc
     6241 ctcgagcttg ttgccggcga gaacttcgcg gccacggaca tcggctggta cggactcagc
     6301 agtacacgct ggccatcctc gggaatcatc agcagggaag ccgccgccac gcttttcccc
     6361 gacacaccgc tgaacgaagt ggtcgggaag accatataca taaacagcga tgatgacgtc
     6421 cgcatcgtcg gaatcgtgga acggttgcag gcggcgtgga aaagctggga cggtgtcgag
     6481 cggtccatgc tggtcccggc cttcctcgag gggagcggca cgcggtatgc catccggacg
     6541 gagcctggtt atcgtgacga gctgatgccc cgggtcgagc agagactcgc ggagatgaat
     6601 gacggccgca tagtccgcga catgatgacg atggatgcaa ctcgcgaccg cgcctatcac
     6661 gaggacgcgg cgatgat
//
LOCUS       DASUOM010000294         4666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1581015,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000294 DASUOM010000000
VERSION     DASUOM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581015"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..80
                     /locus_tag="VFY27_06200"
     CDS             <1..80
                     /locus_tag="VFY27_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5765140.1"
                     /translation="EEGLRFAIREGGRPVGAGVVAKIIE"
     gene            154..229
                     /locus_tag="VFY27_06205"
     tRNA            154..229
                     /locus_tag="VFY27_06205"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Trp,seq:cca)
     gene            286..666
                     /gene="secE"
                     /locus_tag="VFY27_06210"
     CDS             286..666
                     /gene="secE"
                     /locus_tag="VFY27_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5765141.1"
                     /translation="MNAHTETSESGVVDILKLLIAAGVLVGGLFGYYWYLEWSQPLRV
                     LLVLAGTIAGVGIAMTSTQGRRLWAFIQGSRIEIRKVVWPTREETTQTAIAVFVFTLI
                     LALFFWGLDSFLLWLTRTLVGSTV"
     gene            690..1223
                     /gene="nusG"
                     /locus_tag="VFY27_06215"
     CDS             690..1223
                     /gene="nusG"
                     /locus_tag="VFY27_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5765142.1"
                     /translation="MPLRWYIVHAYSNFEHKVKSSLEERIALMGLKDKFGEILVPTEE
                     VVEMKEGQKRRSERKFFPGYVLVQMEMDDETWHLVKEVPKVLGFIGGSSDRPAPITEK
                     EANAILQRVKEGVDKPRPKVLFEPGEVVRVIDGPFNDFSGVVEGVNYEKSRLQVAVQI
                     LGRSTPVELDFGQVEKG"
     gene            1347..1781
                     /gene="rplK"
                     /locus_tag="VFY27_06220"
     CDS             1347..1781
                     /gene="rplK"
                     /locus_tag="VFY27_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5765143.1"
                     /translation="MAKKVTGYIKLQVPAGQANPSPPVGPALGQHGVNIMEFCKAFNA
                     ATQGTEAGMPIPVVITVYSDRSFTFVTRTPPAAVLLRKAAGVPKGSGTPNTSKVGKVT
                     RQQLEEIATTKTPDLTAADMDAAVRTIAGTARSMGLDVEGVN"
     gene            1781..2476
                     /gene="rplA"
                     /locus_tag="VFY27_06225"
     CDS             1781..2476
                     /gene="rplA"
                     /locus_tag="VFY27_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5765144.1"
                     /translation="MARLTKRRKLAREAVKPASVYQIDEALALVKQFANAKFAESVDV
                     SINLGVDPRKSDQVVRGSTVLPNGTGKTVRVAVFAQGENAEKARAAGADIVGFEDLAE
                     KVKAGEMNFDVVIATPDAMRVVGQLGQILGPRGLMPNPKVGTVTADVEGAVRNARAGQ
                     VRYRTDKAGIIHCPIGRADFEVPALKENLNALLADLRKAKPSAAKGAYVRKLTVSSTM
                     GPGVSVDQASIEA"
     gene            2765..3286
                     /gene="rplJ"
                     /locus_tag="VFY27_06230"
     CDS             2765..3286
                     /gene="rplJ"
                     /locus_tag="VFY27_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5765145.1"
                     /translation="MALRLEDKKLFVKEVNAVAGDSLSAVAAEYRGLTVSQMTALRKE
                     ARNAGVYLRVVKNTLARRAIEGTEFECMKDTLKGPILLAFSKDDPGAAARIIRSFSKD
                     HDALKAISLSAGGQLLPASDLAKLAELPTLDQARSMLLGVMLAPMSKLVRTLAEPSAM
                     LARTLAARGEQTA"
     gene            3351..3734
                     /gene="rplL"
                     /locus_tag="VFY27_06235"
     CDS             3351..3734
                     /gene="rplL"
                     /locus_tag="VFY27_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5765146.1"
                     /translation="MALSNQELLKELSAKPIMEVVELVKMLEDEWGVSAAAPVAVAAA
                     GAGAGEAAAAEEEKTEFDVVMTSFGANKVAVIKAVRTITGLGLKEAKDAVEGAPSVIK
                     EGASKADAENFKKQLEEAGATVELK"
     gene            4039..>4666
                     /gene="rpoB"
                     /locus_tag="VFY27_06240"
     CDS             4039..>4666
                     /gene="rpoB"
                     /locus_tag="VFY27_06240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5765147.1"
                     /translation="MAYTFTEKKRIRKNFGKRPTILEVPYLLSIQLDSYRKFLQVDQG
                     DADRSDIGLHAAFKTVFPIVSYSGNAALEYVSYRLGEPVFDVKECQMRGLTYAAPIRV
                     KVRLVIYDKEASGTPKPVKDIREQEVYLGEMPLMTDHGTFVINGTERVIVSQLHRSPG
                     VFFDHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDALFARIDRRRK"
BASE COUNT         1009 a         1261 c         1526 g          870 t
ORIGIN      
        1 tggaagaagg cctgaggttc gcgattcggg aaggggggcg cccggtgggc gccggcgtgg
       61 tggcaaagat catcgagtag gtcaggcgga tatgtttggg gtcagacccc attttttgga
      121 aaaaaatggg gtctgacccc atatccactc agtaggccag tagctcaatt ggcagagcgg
      181 cggtctccaa aaccgcaggt tgggggttcg attccctcct ggcctgccac ttgagcttga
      241 actccgccgc cgcggcgggg cgggccggat tgacattcga ttttgatgaa cgcacatacg
      301 gaaacgagcg aatcaggcgt cgttgacata ctgaagctgc tgatcgcggc cggcgtgctg
      361 gtgggcggcc ttttcggtta ctactggtat ctcgaatggt cgcagccgct gcgggtgctg
      421 ctggtgctcg ccggcacgat cgcaggcgtc ggcatcgcga tgaccagcac ccagggcagg
      481 cgcctgtggg cgtttatcca gggatcgcgc atcgagatcc gcaaggtcgt ctggccgacc
      541 agggaggaga cgacccagac cgccatagcg gtgttcgtgt tcacgctgat cctcgccttg
      601 tttttctggg gcctggattc atttctgctg tggctgacga gaacgctggt cggttcgacg
      661 gtctgacatg gaattaaaga ggtaatcagg tgcccttacg ctggtacatc gttcacgcct
      721 attccaactt cgaacacaag gtaaagagtt cgctggagga gcgcattgcg ctcatggggc
      781 ttaaggacaa gttcggtgag attctcgtgc cgaccgagga agtggtcgag atgaaggaag
      841 gacagaagcg gcgcagcgag cgcaagttct tccccggcta cgtactggtg cagatggaaa
      901 tggacgacga gacctggcac ctggtgaagg aagtaccgaa agtgctcggc ttcatcggcg
      961 ggtccagcga ccgcccggcg ccgattaccg agaaggaagc gaatgcgatc ctgcagcggg
     1021 tcaaggaagg cgtggacaag ccgcgcccga aggtactgtt cgagccgggc gaggtcgtgc
     1081 gggtcatcga tgggccgttc aatgatttca gtggcgtggt cgagggcgtg aactacgaga
     1141 agagccggct gcaggtggcg gtgcagattc tcggccgctc gacaccggtg gagctggatt
     1201 tcggccaggt agagaaagga taggttttcg cgaccagcta agagcgattt aaacggggag
     1261 ccctgaccag gcgttcatac ccaacacagt aaatgcaaca tcggggagcc tcaggtcagg
     1321 cgccagcacc cgagaaggta gatgacatgg ctaagaaagt tacgggttac atcaagctgc
     1381 aggttcctgc aggccaggca aatccgagtc cgcccgtcgg tcccgcactt gggcagcatg
     1441 gcgtgaacat catggagttc tgcaaggcgt tcaatgccgc cacgcagggc accgaagcgg
     1501 gtatgccgat cccggtggtt atcacagttt atagcgatcg gagcttcacg ttcgttacga
     1561 ggacgccgcc ggccgcggtg ctgctgagaa aagccgccgg agttccaaag ggttccggga
     1621 cgccgaacac gagcaaagtg ggcaaagtga cgcgccagca gctcgaggaa atcgccacga
     1681 cgaagacgcc tgacctgact gcagccgata tggatgccgc ggtacggacg atcgcaggca
     1741 cggcccgaag catgggcctc gacgtcgaag gagtgaactg atggccaggc tgacgaaacg
     1801 caggaaactt gcccgcgagg ctgtgaagcc ggcatcggtt taccagatag acgaggctct
     1861 tgccctggtc aagcagttcg cgaacgcgaa gttcgcagaa agcgtggacg tgtccatcaa
     1921 tctgggcgtc gacccacgca agtcggacca ggtcgtacgc ggttctaccg tattgccgaa
     1981 cggcaccggc aagacggtgc gcgtggccgt gtttgcgcag ggcgagaatg ccgaaaaagc
     2041 aagggctgcc ggcgccgaca tcgtaggttt cgaggacctt gccgagaagg tgaaggcggg
     2101 cgagatgaat ttcgacgtcg tcatcgcgac gccggacgcc atgcgggtgg tcggccagct
     2161 cgggcagatc ctgggtcctc gcggcctgat gccgaacccg aaagtcggca ctgtgaccgc
     2221 cgacgtggaa ggcgctgtaa ggaatgcgag ggccgggcag gtgcggtacc gcacggacaa
     2281 ggcaggaatc attcattgcc cgatcgggcg ggcggatttc gaggttccgg cgctcaagga
     2341 gaacctgaac gcgctgttgg cggacttgag gaaggccaag ccgtccgcag cgaagggcgc
     2401 atatgtgagg aagctgacag tgtcctcgac catgggtccg ggagtgtcgg tcgaccaggc
     2461 aagcatcgaa gcctgatcga gaaagttttt tgcctcggcc ggcttccggc cggggtgaaa
     2521 atgggaccgg cttgccggtc cgcgtcagag accgtaggcg actgagtcga taagaccggt
     2581 cttaattgcg aagcggccta cgcagatggt gttacctgag tcagagcttt tctggcgaag
     2641 tcaccgtcgc aaagccaggg cggttcgaac ggggttcttg ctccggccgc tgccccggaa
     2701 tgggtaggca aagaggaatt ttgcctttgt gtgaccgtaa gccagtgaag tccaggagaa
     2761 gttcatggca ctgagactcg aagacaaaaa gctttttgtc aaagaggtaa acgcggttgc
     2821 tggcgactct ctaagcgctg ttgcagcgga atatcgaggg ttgacagttt cgcaaatgac
     2881 ggcattacgc aaggaagcgc gtaacgccgg cgtgtacctg cgggtggtta aaaacacgct
     2941 ggcgcgccgg gccatcgaag gcaccgaatt cgagtgtatg aaagacacgc tcaaggggcc
     3001 gatactgctc gcgttttcga aggacgatcc gggcgcagct gcgagaatca tacgcagctt
     3061 ctccaaggat cacgatgctc tgaaggcgat ctcgctctcg gctggcgggc aattgctgcc
     3121 ggcgagcgat ctggcgaagt tggcggaact gccgacgctg gaccaggcgc gttcgatgtt
     3181 gctaggcgtc atgctggcac cgatgagcaa actcgtccgc accctcgccg agccgtcggc
     3241 aatgctggcc cgtaccctcg cggcacgagg cgagcagacg gcttaaaggc gaatcgtcgt
     3301 caagaaaact ctttttatct gattcagacc taatctcaag ggaaattaaa atggcacttt
     3361 ctaaccaaga actgctcaaa gaactgagcg caaagcccat catggaagtc gtcgagctcg
     3421 tcaagatgct cgaggacgag tggggcgtat cggccgccgc cccggttgcg gtcgccgctg
     3481 ccggcgccgg tgccggtgaa gccgctgccg cagaggaaga aaagaccgaa ttcgacgtgg
     3541 taatgaccag cttcggcgcc aacaaggttg ccgtcatcaa agcggttcgc accattaccg
     3601 gtctgggcct caaggaagca aaggacgccg tcgaaggcgc accctccgta atcaaggaag
     3661 gcgcctccaa ggccgatgcc gagaacttca agaaacagct tgaagaagcc ggcgctacag
     3721 tagagctcaa gtagtagaga ttgacgcatc tcctcattga tgctggcccg gttatccggg
     3781 ctctggcccc gcaacacggg gcctacaagc cggagaggca gccggcgggt cggtggatcc
     3841 gcgtggctgc ctcgccgtgt gcgtgccttt tgaaggtgcc ggcggcgaag atgagccgta
     3901 aataccggtc tgcgaactcg tatcgcgaaa tttgaaattt tgaaatccgc ggaagttttg
     3961 aacgttcttg atttctgcac tggtcgcttg cctgaaggca ggcccagcca accgcttccc
     4021 aagcgagggt aatcccgaat ggcttacaca ttcactgaga aaaagcgcat ccgcaagaat
     4081 tttggcaaac ggccgacgat tctcgaggtg ccttacctgt tgtcgatcca gctcgattcc
     4141 taccgcaagt tcctgcaggt ggatcagggc gatgccgatc gcagcgacat cggcctgcac
     4201 gcggcattca agacggtgtt cccgatcgtg agttactccg gcaatgcggc gctcgagtac
     4261 gtcagctacc gtctcggcga gcctgtgttc gatgtgaagg aatgccagat gcgcggcctt
     4321 acctacgccg ccccgattcg cgtcaaggtt cgcctggtca tatacgacaa ggaggccagc
     4381 ggcacgccga agccggtcaa ggatattcgc gagcaggaag tgtacctggg cgaaatgccg
     4441 ctgatgacgg atcacggaac gttcgtgatc aacggcaccg agcgcgtgat cgtatcgcag
     4501 ctgcaccggt cgccgggcgt cttcttcgac cacgacaagg gcaagactca ctcgtcgggc
     4561 aagctcctgt tttcggcgcg gatcatcccg taccgtggct cgtggctgga tttcgagttc
     4621 gacccgaagg atgccttgtt cgctcgtatc gaccggaggc ggaagc
//
LOCUS       DASUOM010000295         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1508819,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000295 DASUOM010000000
VERSION     DASUOM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508819"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="pheA"
                     /locus_tag="VFY27_06245"
     CDS             <1..754
                     /gene="pheA"
                     /locus_tag="VFY27_06245"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5765148.1"
                     /translation="SVRALPFATIDEVFREVESGAADFGVVPIENSTEGTVNNTLDMF
                     LTSPLKIGGEIELGIEQHLLGRMPSLEKIERVCAHEQALAQCRGWLREYLPHVDLIGV
                     SSNAVGARRARDEQGTAAIGGETAAEVYNLEYLVRNIEDRPDNATRFLVIGRELLAAS
                     GDDKTTVLVSTADTAGGAGVLHALLQPFATHGVNMTRIESRPSRRKNWDYVFFVDLDG
                     HAGQPPLSDALRELKEISSLYRVLGAYPKAVG"
     gene            772..2085
                     /gene="aroA"
                     /locus_tag="VFY27_06250"
     CDS             772..2085
                     /gene="aroA"
                     /locus_tag="VFY27_06250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5765149.1"
                     /translation="MNYVVTPAPLRGGSIAVPGDKSISHRALMLGAIAEGDSLVTGFL
                     AGEDCKATLAALRSMGVTVEELDQTTLKISGVGMHGLQAPATALDMGNSGTAMRLFTG
                     LLCGQAFAATLSGDASLSRRPMGRVIHPLSRMNASIESNNGLPPLTIHGGRKLTGINY
                     SLPVASAQVKSAILLAALYAEGDTTVGEPAITRDHTERMLQYFGVKLQAENGRIRVAG
                     KQVLRGAGLRVPGDLSSAAFVMLGAILAPECEVLLKGVGVNPTRSGIIEILRQMGAAI
                     EIEPRGTHGLEPVADIRVRSSVLRGIDVEPALVSLAIDEFPVLFIAAAAAKGRTRFTG
                     IGELRVKESDRISTMAEGLRKLGIRVQETDDGASVLGGSFTGGTVESHGDHRVAMSLA
                     VAGTVAASPVTVLNTDAVDTSFPGFVDCMKTAGADISIRAQHVFT"
     gene            2097..2783
                     /gene="cmk"
                     /locus_tag="VFY27_06255"
     CDS             2097..2783
                     /gene="cmk"
                     /locus_tag="VFY27_06255"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5765150.1"
                     /translation="MPAGPPVVAIDGPSGSGKGTVARRVAEALGWHLLDSGALYRLTA
                     LAASRSGKSDAPESVLAQIAANLDVRFAVDSAGQEQVWLARENVTADVRTEECGRLAS
                     RVAPLPAVREALLGLQRGFRRPPGLVADGRDMGTEVFPDAILKVFLTASAEERARRRY
                     KQLKDKGIDVSLPALSQHIEDRDRRDSERTVAPLRAAEDARLLDSSNLTIDEVARIVL
                     AWVQERLPAV"
     gene            2912..4597
                     /gene="rpsA"
                     /locus_tag="VFY27_06260"
     CDS             2912..4597
                     /gene="rpsA"
                     /locus_tag="VFY27_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5765151.1"
                     /translation="MSESFAELFEESFASQHIKPGSIITGRVVAVNADVVIVSAGLKS
                     EAVIPIEQFQNEKGQADVNVGDEIEVALDAVEDGFGETRLSREKAIRARTWTELEKAF
                     EKGEVVQGVINGRVKGGFTVEIDNVRAFLPGSLVDVRPVRDPAYLEGKSLEFKVIKLD
                     QRRNNVVVSRRAVVEQEYSAEREELLESLQEGNVVKGIVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVKHPSEVVNVGDEIEVKILKFDRERQRVSLGIKQLGDDPWHDLARRYPP
                     GARIFGKITNIADYGCFVEIEEGVEGLVHVSEMDWTNKNVNPAKVVQVGQEVEVMVLE
                     IDEERRRISLGIKQCQPNPWAEFSATVNRGDKVSGQIKSITDFGIFIGLEGGIDGLVH
                     LSDISWDQPGEEAVHNYRKGQEIEAAVLAIDAERERISLGIKQLDKDPFSGWLAEHPK
                     NSVVKGVVTEVDARGAVVDLGDGVTGTLRASELARGRIDDARTVLKVGDEIEAKFTNV
                     DRKNRSVALSVKAKEVHEEAEALSTYKSDAGTTPTGTTLGELLKEKMTGGADH"
BASE COUNT          979 a         1334 c         1548 g          875 t
ORIGIN      
        1 ctcggtccgt gcattgccgt tcgccaccat cgacgaggta tttcgggaag tggagagtgg
       61 tgccgccgac ttcggtgtcg tgccgatcga gaactccacg gaaggcacgg tcaacaatac
      121 gctcgacatg tttctcactt cgccgctgaa gattggcggg gagattgaac tcggcataga
      181 acagcacttg cttggccgca tgccgtcgct cgagaagatc gagcgcgtct gcgctcacga
      241 acaggcgctt gcccaatgcc gggggtggct gcgggaatac ctgccgcacg tcgacctgat
      301 cggcgtcagc agcaacgccg tcggggcgcg ccgcgcacgc gatgaacaag gtacagctgc
      361 catcggcggc gaaactgccg cggaggtcta caacctcgag tacctggtac gcaacatcga
      421 agaccggccg gacaacgcga cgcgctttct cgttatcggc agggagctgt tggcggcgag
      481 cggtgatgac aagaccacgg tcctggtttc aacagccgac acggcaggcg gcgcaggtgt
      541 gctgcacgca ttgctgcagc cgtttgcaac tcacggcgtg aacatgactc gcatcgaatc
      601 gcgcccctcg cggcgcaaga actgggacta tgtgtttttt gtcgacctcg atggccatgc
      661 ggggcagccg ccactatcgg acgcgttgcg cgagctgaaa gagatcagct cgctgtaccg
      721 ggtgctcggg gcctacccga aagccgtcgg ttagtctcgg cgggtaccca gttgaactac
      781 gtcgtcacgc cagcgcccct gcgaggcggg tcgatagccg tgccgggcga taaatccatc
      841 tcccatcgcg cattgatgct cggtgcgatc gccgagggcg atagcctcgt aaccggattc
      901 cttgccggtg aggattgcaa ggcgacgctc gccgctctcc gaagcatggg agtcacagtg
      961 gaggaactgg accagacgac cctgaagatt tccggcgtcg gcatgcacgg cctgcaggcg
     1021 ccggctacag cgcttgacat gggtaattcg ggaacggcaa tgcgtctctt caccggcttg
     1081 ctgtgcggac aggcatttgc cgccacgctt tcgggtgacg cctcgctctc gagacggccg
     1141 atggggcggg taatccaccc tctcagccgg atgaatgcca gcatcgagag caacaatggt
     1201 ttgccgccgc tgaccattca tggaggccgc aagctcaccg gaatcaacta ctcgctgccg
     1261 gtggcgagcg cacaggtcaa gtccgctatc ctgctcgccg cgctctacgc cgagggtgac
     1321 accactgttg gcgaacctgc gatcacccgg gatcatacgg agcgcatgct gcagtatttc
     1381 ggcgtgaagc tgcaggcgga gaatggccgc atccgggttg ccggaaagca agtcttgcgt
     1441 ggcgcaggcc tccgcgttcc cggcgatctc tcgtccgcgg cattcgtgat gctcggggcc
     1501 attctggcgc cggaatgcga ggtactcctg aaaggggtgg gcgtaaaccc gactcgctcc
     1561 ggaatcatcg agattctccg tcagatgggc gcggcaatcg aaatcgaacc gcgcggaacg
     1621 cacggtctgg aaccggtcgc cgatatacgg gtcagatcgt ccgttctgcg gggcatcgat
     1681 gtggagcccg cgctcgtgtc gctcgcgatc gatgagttcc ctgttctttt tattgccgcc
     1741 gccgccgcca agggccgcac gcggttcact ggtatcggcg aactgcgcgt caaggaaagc
     1801 gaccgcatca gcacgatggc ggagggactg cgcaagttag gtatccgcgt ccaggaaacg
     1861 gatgatggag ccagcgtgct tggcgggtct ttcaccggcg gaaccgttga aagccatggc
     1921 gaccaccgcg tggcgatgtc cctggcagta gccggcacgg ttgccgcttc gccggtgacc
     1981 gtgctgaata ccgatgctgt cgacacgtcc ttccccggct tcgtggactg tatgaaaact
     2041 gccggagccg acatctcgat ccgcgcgcaa cacgtgttca cgtgagatcg cagcccctgc
     2101 cggcaggtcc ccccgtggtt gcgatcgacg gacccagcgg gtcgggcaag ggcacggtcg
     2161 ccaggcgcgt cgcagaggcg ctggggtggc acctgctgga cagcggcgcc ttgtaccgcc
     2221 tgacggccct ggcagcctca cgaagcggca aaagcgacgc gccggagagt gtcctggcgc
     2281 agattgcggc gaatttggac gtccgtttcg cggtggattc ggccgggcag gagcaggttt
     2341 ggctggctcg ggagaatgtg acggccgatg tgcgtaccga ggaatgcgga cgccttgcct
     2401 cgcgcgttgc ccccttgcct gcggttcgcg aggccttgct gggcctgcag cgcggctttc
     2461 gcaggcctcc cgggctggtc gcagacggcc gggatatggg caccgaggtg ttcccggatg
     2521 caatcctgaa agtgttcctc accgccagtg cggaggagcg cgcacgcagg cggtataagc
     2581 agttgaaaga caagggtatt gatgttagcc ttcccgccct ttcgcagcac atcgaagatc
     2641 gcgacaggcg ggattcggag cgaacggtcg ctcccttgcg cgctgctgaa gacgcccgat
     2701 tgctggactc cagcaacctg acgattgatg aagttgcccg gatcgtgctg gcctgggtgc
     2761 aggagcgatt gcctgcagtt tagttattgc agttggtttt cacggacccg gacagggtag
     2821 tccgggtcat tcttaggtgt aaccgttcgc cgcaggaagc ggtggaacga atgggttgag
     2881 gatcaaaaca aggtcctctg gtttaaatca catgtctgaa agtttcgctg aattatttga
     2941 agagagtttt gccagtcaac acataaaacc cggttctatc attaccggca gggttgtcgc
     3001 ggtgaatgct gatgtcgtta tcgtcagcgc cggcctcaag tccgaggccg tcattcccat
     3061 agaacaattt caaaacgaga aaggtcaggc cgatgtcaat gtcggcgacg agatcgaggt
     3121 agctctcgat gccgtcgaag acggattcgg tgaaacacgc ctttcgcgcg agaaagctat
     3181 ccgcgccagg acgtggacgg agctcgagaa agccttcgag aaaggggaag ttgtacaggg
     3241 cgtcataaac ggcagggtca aaggtggctt tacggtcgag atcgacaacg tgcgggcgtt
     3301 cctgccgggg tccctggtcg acgttcgccc ggtgcgggat cccgcgtatc tggaaggcaa
     3361 gagcctggag ttcaaggtca tcaagctcga ccagcggcgc aacaacgtcg tcgtgtcgcg
     3421 gcgtgcggtc gttgaacagg agtacagcgc ggagcgcgag gaactgctcg agagcctgca
     3481 ggaaggcaac gtggtcaagg gcatcgtcaa gaacctcacc gattacggcg catttgtcga
     3541 cctgggtggc atcgacgggc tgctgcatat caccgacatg gcgtggaagc gagtcaagca
     3601 cccgtcggaa gtggtcaacg tcggcgacga gatcgaagtc aagatcctca agttcgaccg
     3661 cgaacgccag cgcgtctcgc tcggcatcaa gcagctgggc gacgatccat ggcacgacct
     3721 tgcgcggcgc tacccgccgg gcgcccgtat cttcggcaag atcacgaaca tcgccgatta
     3781 cggctgtttc gtggaaatcg aggaaggtgt cgagggcctg gtccacgtgt ccgagatgga
     3841 ctggacgaac aagaacgtca atccggccaa ggtggtgcag gtaggccagg aagtcgaggt
     3901 gatggtcctc gagatagacg aggaacggcg ccggatttcg ctcggcatca agcagtgcca
     3961 gccgaatccg tgggcggagt tctcggcaac cgtgaaccgc ggcgacaagg tttccgggca
     4021 gatcaagtcc atcaccgact tcgggatctt catcggcctg gaaggcggca tagacggcct
     4081 cgttcacctg tcggatatct cctgggacca gccgggtgaa gaagccgtac acaactatcg
     4141 caagggacag gagattgaag ccgcagtgct ggcaatcgac gccgaacgtg aaaggatctc
     4201 acttggcatc aagcaactgg acaaggatcc cttctcgggc tggcttgccg agcatccgaa
     4261 gaactccgtc gtgaaaggcg tggtgactga ggtggatgca cgtggagcgg tggttgacct
     4321 gggtgatggc gttaccggga cacttcgagc ctcggagctt gcgcgcggac gcatagacga
     4381 tgcgcgcacc gttctgaaag tcggtgatga aatagaagcg aagttcacga atgtagatcg
     4441 caagaacaga agcgttgcgc tgtcggtgaa ggcgaaggaa gttcatgaag aagcggaagc
     4501 gctcagcacc tacaagtccg atgccgggac gaccccgacg ggaacaaccc tcggagaact
     4561 gctgaaagaa aagatgacgg gcggcgctga tcattaaaaa aagactggcc cggaccaggt
     4621 ggtccgggct atatttcttt tgtaatcgcc gggttacagg gacaggtatc agatacaaaa
     4681 agcgaagcgt gttatgacaa aatctgaact catcgaaatg atcgcaagga agcaga
//
LOCUS       DASUOM010000296         9593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1270722,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000296 DASUOM010000000
VERSION     DASUOM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270722"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFY27_06265"
     CDS             <1..256
                     /locus_tag="VFY27_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765152.1"
                     /translation="MYYFDSLQGQSRRTRLRARLTGWRDKVTGKLSTIGRKLPGKAED
                     TTDEVAEEITNQMTGNGSADSLRTQDRLQDRLLQDRPLQG"
     gene            412..675
                     /locus_tag="VFY27_06270"
     CDS             412..675
                     /locus_tag="VFY27_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5765153.1"
                     /translation="MNIIVWLVVGGLIGWVASIIMKTNDRQGILLNVIVGIVGAVLGG
                     WLFSGVFGGGSINEGDFSVSGLLVSLLGAVILLFVVKLVRRST"
     gene            888..3338
                     /locus_tag="VFY27_06275"
     CDS             888..3338
                     /locus_tag="VFY27_06275"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5765154.1"
                     /translation="MAAKKTKTGSKAPKSKARSKDPTGTGQSSAKTSARSTVSGPTHK
                     REAVDMSDAATAKMEGTEMVSASFPYNAAKASEFGNASKEPAVGQSVEPPHPLVGAST
                     LSEGNASEKVGKGNPQVGFNPGNGPLDRVRVDSSGRVLTTNQGVPVADNQNSLKAGLR
                     GPTLLEDFILREKVTHFDHERIPERIVHARGSAAHGFFQCYESLADLTRASIFAEAGK
                     RTPVFVRFSTVAGERGSTDTARDVRGFAVKFYTDEGNWDLVGNNIPVFFIQDAMKFPD
                     LVHALKPEPHHAMPQAASAHDTFWDFVSLMPESTHMLMWVMSDRAIPRSYRMMQGFGV
                     HTFRFVNAQGESHFVKFHWTPLAGTHSLAWDEAVKISGADPDFHRRDLWEAIEAGVGP
                     EYELAVQVFTEEQADGFSFDVLDATKLIPEELVPLRPIGRLVLNRNPDNFFAETEQVA
                     FCSAHIVPGLDFSNDPLLAGRIHSYVDTQISRLGGPNFHEIPINASIAQVHNNQRDGM
                     HRQAIPRGRVAYEPNSLGGGCPFQAGVQGFMSFPQPSEGDKVRGKPERFAEHYNQATL
                     FWESQTEVEKLHIVRAFRFELTKVQTVAIRTRMVAGLRNVAEELAQGIADGLGIELPA
                     ALPKLIKRSPKPEIRTSAALSLLARPGQTGILTRRIAILVADGVAGRGVIALHHALAE
                     QGAVPRFVGIKLGQVSSADSDPIEVEISMETGASVLYDALVLPDGEQGVTALSQSGHA
                     MEFLKDQYRHCKPILALGVGNALLERAGIPDSLPSGENDGGILRFAGVDADAAFKDFA
                     QAIMKHRNFERETDPPLI"
     gene            3387..3725
                     /locus_tag="VFY27_06280"
     CDS             3387..3725
                     /locus_tag="VFY27_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5765155.1"
                     /translation="MAKVAKKKRSDAISLLKADHRKVEGLFKRYEKAKAESVKAKLAH
                     EICLELSVHSTIEEELFYPAIKETVEEDIYDEAYVEHDGAKVLIAEILEGTPADDFYV
                     AKVTVLSEMI"
     gene            complement(4032..5261)
                     /gene="cls"
                     /locus_tag="VFY27_06285"
     CDS             complement(4032..5261)
                     /gene="cls"
                     /locus_tag="VFY27_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5765156.1"
                     /translation="MLVTVFALNFVGGEKKIERKLEHRYGIDDPQFLRELSILLGPPI
                     IEGNRVTNLENGDEIFPAMLAAVREARQTINFETYIYWSGEIGREFAAALAERAAAGV
                     DVHVLIDWVGGQKMDDSILETMIEAGVKVERFHPLRWYNLGRMNNRTHRKLLIVDGHV
                     GFTGGVGIADIWKGNAESSEHWRDSHYRLEGPAVAHMQAAFIDNWIKTTGHVLQGEEY
                     FPALEPVGNARAQVFTSSPSGGGDSMLLMYLMSITAAERTIDLSAAYFVPDDLMRQAI
                     RSALERGVRVRIIVPGTHIDTEVVRKASRAVWGELLEAGAEIHEYSPTMFHCKMLVVD
                     RRMVSVGSTNFDNRSFRLNDEANLNVYDQAFAEEVTAVFERDLEHSDRISHDKWLRRP
                     WQEKLVEHTSALLSSQL"
     gene            complement(5654..6652)
                     /locus_tag="VFY27_06290"
     CDS             complement(5654..6652)
                     /locus_tag="VFY27_06290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5765157.1"
                     /translation="MSTVSVPQHAEELAVSVIMPVYNGARFLPAAVESVLAQTLPNFE
                     VLIIDDGSVDDSARVAERFAARDPRVQLLRQAGNRGQAAARNLALERARGIWIAPVDA
                     DDEINPDRLRLLVEAGEREHADLIADGILFQGARPPGTPAELMTWRRNGHDVELLSAE
                     ALIKSDIPAGGHCSLGYLKPLMRRRFLDECNLRYAEDLRFAEDIHLYVQALFCGARFI
                     LYPESHYVYRQTPASASRTEMLLPRMARHAVSGSQRLRASVPHPGSAELIGALDEHEQ
                     RWSLLFWFEQLKRYVADRHYWRAAKHLFQRPASAGRLIRFASDRARMKRSLPGPTA"
     gene            complement(6680..8284)
                     /locus_tag="VFY27_06295"
     CDS             complement(6680..8284)
                     /locus_tag="VFY27_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5765158.1"
                     /translation="MPNHQDTALPGARRSDTERAGPRTGGNGQRKLVIVGVDAGSPEL
                     FRKWCATGDLTHISRLMAAGSTRRVENPRGMEAGAVWPVFQTGLLPGRQPMYDGRRNF
                     NSQTYVERWYGPEETAPTVWRQLSDAGLSCLLIDPPYTLVDPQINGVTIMDWGTHVPA
                     NGNTFEFKTHPPEVAAEVLALVGPDPARGVMCDVMSPESLDDYRRFRDIYLRRIEGKA
                     ELAHHFLKSTDWDVALVTSSDLHCAGHHLWHINEPRHPRYSAKVEAELGEPLRDCYRA
                     FDRSLETILAAIDAQTTVMLFGSHGMGPSFSGTGLLDRILLRLDKGTGHVAPHSRKAR
                     LRALWHRVPIEMRGRLKLLRRPFRGALHPPKFLGDHANRRFFEVYANNASGGVRLNVK
                     GREGSGTVEPGETDALLRFLTTELMKVVNADTGERLIKDIVVTRKEYSGPYLERLPDL
                     LVTWNRNAPIERVRSDTIGELYREHLDNRTGDHTPDGICIIAGHGADFCGEAPAIKTA
                     DLAPSIAHFFGVELRDHDGRMFELTV"
     gene            complement(8371..>9593)
                     /locus_tag="VFY27_06300"
     CDS             complement(8371..>9593)
                     /locus_tag="VFY27_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765159.1"
                     /translation="EEISRWDFPSLAAVPNAVSVLEDEVYIGGYDLETDRGITGRLSG
                     APLLKPALARLDGSIPGSAERLAVLDHHYGAGPVAARVQVPGSDTGNTVFFGDGLRPV
                     DFFAAEDMDGNGHDELVVLSKVPPVVEIVDNESGDQILRLELDDDFEPIAAVIANEGA
                     TSVLAVLHKHSTKNYVRASIFDAATGVGLGTIAYNPAFTPLDLYALPSASGLRYAVLA
                     VDTSGTSPDKLEIRNPDGSLEANLWLGTEMTPLRGLQYEDGGASRIAVLRQNAEQGWL
                     DIEVVDPDVGVLSKLPFNAAFAPDDFEHSPDVDANLSPQFTLAGRNDVGKVKAETRDL
                     ATGALLHNVFLQPFELIEDVIYLAPGQGVSTPSLALLTRDDDIISPQSQFRVVLVDLL
                     TGSEVDDWFFDFAN"
BASE COUNT         1975 a         2874 c         2847 g         1897 t
ORIGIN      
        1 gatgtattac tttgattcct tacaaggcca gtcccgccgc acacgcttgc gcgcccggct
       61 caccggctgg cgtgacaagg tgacaggaaa gttgagcacc attggccgga agttgccagg
      121 caaagctgag gacaccactg atgaagttgc agaggagatt acgaatcaaa tgaccggcaa
      181 cggatccgcc gatagcctcc gaacgcagga tcgtctgcag gaccggctgc tgcaagatcg
      241 tccgctgcag ggctaaggaa acagggactg tgatcctgtt ctccgcgaac acgtttcgta
      301 cgtgatcaca aacatcgacc gtgagggttc atggtgcggc agcgtacaga ctgacgccgg
      361 gtcgaaggtt acggtgatcg ttcaacattc aacacctcaa ctggagcacc tatgaacatc
      421 atcgtttggc tcgtggtcgg cggactcatc ggctgggtcg ccagcattat catgaaaacg
      481 aatgaccggc agggcattct tctgaatgtc atcgttggta tcgtcggcgc agtgctggga
      541 ggatggttgt tcagcggcgt tttcggcggc gggtcgatca atgagggtga cttcagcgtc
      601 agcggtttgc tggtctcctt gctcggtgcc gtgatcctgc tgtttgtcgt caagttggta
      661 cgtcgtagta cttgatggtg ccacgaaggc cgcgttgcaa aggccgagtc aacaggagga
      721 attgctctac caggcgctcg aaatcgagat cggcggcttc gacatttata ccaggcggtc
      781 caattcgcgg tgaatgaaca atctccagaa agatagggaa ggagtacgaa cagacgaagg
      841 ccatatgtgt aggccgcctg cataagtatc cacttcacga gggacacatg gctgcaaaga
      901 agacgaagac cggctcaaaa gctcccaaat cgaaagcgcg gtcgaaggac ccgacgggca
      961 ccgggcaaag cagcgctaag acgtctgccc gatcgaccgt cagcgggccc acccataaac
     1021 gcgaagccgt tgatatgtcg gacgccgcca ccgcgaaaat ggaaggcacg gagatggtgt
     1081 cggcgtcctt tccctacaac gctgctaaag cctctgagtt tggcaacgca tcgaaagaac
     1141 cggcggtagg gcaaagcgta gaacccccgc acccgctcgt cggcgccagc acccttagcg
     1201 agggcaatgc gtccgaaaaa gtgggtaagg ggaacccgca agtcggcttc aaccctggaa
     1261 atgggccgct tgaccgagta cgcgtcgatt cgtccggccg cgtcctcact actaaccagg
     1321 gcgtgccggt cgcggacaac cagaattctc tcaaagcagg cttacgcggg ccgacgctgc
     1381 tcgaggattt catcctccga gagaaagtta cccacttcga tcatgagcgc atccccgagc
     1441 gcatcgttca cgctcgcgga tccgctgcgc atggcttttt tcagtgttac gaatcgctgg
     1501 cggatctgac ccgtgcttcg atattcgccg aagcgggcaa gcgcacgccg gtcttcgtgc
     1561 gcttctccac cgtggcgggc gaacgcggat cgacggacac ggcacgtgat gtccggggtt
     1621 tcgccgtcaa gttttacacc gatgaaggca attgggacct ggtaggcaat aacattccgg
     1681 tgttctttat ccaggacgcg atgaagtttc cggacttggt tcatgcgctg aagcccgagc
     1741 cgcaccacgc aatgccgcag gcggcttctg cgcacgatac gttctgggat ttcgtttccc
     1801 tgatgccgga gtccactcac atgctgatgt gggtcatgtc ggaccgtgcc atcccgcgca
     1861 gctaccgcat gatgcaggga ttcggtgttc acacctttcg cttcgtcaac gcgcaaggcg
     1921 aatcgcattt tgtaaagttt cactggacgc cgctcgccgg cacacattca ttggcgtggg
     1981 acgaggcggt caaaatctca ggtgcggatc cagattttca tcgtcgggat ctttgggaag
     2041 ctatcgaagc cggtgtcggt cctgaatatg agctcgccgt acaggtgttc acggaagagc
     2101 aagcggatgg cttcagtttc gacgtgctcg atgctaccaa gctcattccg gaggaactcg
     2161 tcccgctacg gccgataggc cgactcgttt tgaatcgcaa cccggacaac tttttcgccg
     2221 aaacggagca ggtggcgttt tgtagcgctc atatcgtgcc gggactggac ttcagcaacg
     2281 atccattgct cgccggtcgc atccactcgt atgtggatac gcagatctcc cggctgggcg
     2341 gaccgaattt tcatgagatt ccgattaatg cctccatcgc ccaagtgcac aacaaccagc
     2401 gcgacggtat gcatcgtcaa gcgatccccc gaggccgggt tgcctatgag ccgaattccc
     2461 taggcggagg atgtcccttt caagcgggcg tacagggctt catgtcattc ccgcaaccgt
     2521 ctgagggcga caaggtgcgg ggcaaacccg aacgattcgc tgaacactac aaccaggcaa
     2581 ccctcttctg ggaaagccaa accgaggtgg agaagctcca tatcgtccgc gcctttcgct
     2641 tcgagctgac caaggtacaa acggtcgcca taagaacccg tatggtggcg ggactgcgca
     2701 atgtcgcgga agaacttgcc caaggcatcg ccgacggtct gggcatcgag ttgcccgcag
     2761 cgctaccgaa attgattaag cgctctccaa agcccgaaat tagaacgtct gcagcgctgt
     2821 cacttttagc gcgtccgggt cagactggca ttctgacccg ccgtattgcg attctggtcg
     2881 ctgacggtgt agcgggacga ggtgttattg ccttgcacca cgcacttgcc gagcagggcg
     2941 ctgttccccg atttgtcggg atcaagctcg gccaagtctc cagtgcggac agtgatccga
     3001 tcgaagtcga aatctcgatg gaaaccggcg cctcggtatt gtacgacgca ctcgtgctgc
     3061 ccgacgggga acagggcgtc acagcgctct ctcaaagcgg ccacgcgatg gaattcctca
     3121 aagaccaata ccgccactgc aagccgattc tcgcgctggg cgtcggtaac gctttgctcg
     3181 aaagggctgg gattcccgac tcgttgccat cgggcgagaa cgatggcgga atcttgcgtt
     3241 tcgctggcgt cgacgcggac gcagcgttta aagattttgc acaggcaatc atgaaacatc
     3301 gcaacttcga gcgcgaaact gatccgccat taatttagag gcatccactg ccgtcattca
     3361 atccatccaa gcagcaggtg aacaccatgg ctaaagttgc caaaaagaaa cgttcagacg
     3421 caatttctct actcaaggca gaccatcgca aggtcgaagg actcttcaag cgttacgaaa
     3481 aggccaaggc agagagcgtg aaggccaagc tggcacatga gatctgcctc gagctttcgg
     3541 ttcattccac aatcgaggag gagctattct atccggcgat caaagaaacc gtggaggaag
     3601 atatttacga cgaagcttac gttgagcatg acggcgccaa ggtcctgatt gctgagatcc
     3661 tggaggggac gcctgctgac gatttttacg tcgctaaagt gacggtgctt tccgagatga
     3721 tctagcatca tgtcaaggaa gaagagcaac gcggcggtct gttcgcgcag gcacgcgacg
     3781 acggtgatgt cgatctatta gagctcggtg cgctaatggc tgcgcgcaag aaggaactcg
     3841 tggcgaaaat aaagacagca gggattccaa cacccacaac ccgctcaatg aagggagccg
     3901 agctgcagcg cgcggaaccg gttgtctagc cttccagcgg aagaggctgc tcctgatagc
     3961 agggaaacgc acgagcgagt ggataaggac cggccagcga cggactgcct gacgacgaac
     4021 cagcttacgc tctagagctg ggaggacaga agcgcggaag tgtgctccac gagcttctcc
     4081 tgccaagggc gtctgagcca cttatcgtgg ctgatgcgat cagaatgttc gagatcgcgc
     4141 tcaaacaccg cggtgacttc ctcggcaaag gcttggtcgt agacgttaag attcgcctcg
     4201 tcattgagac ggaatgaccg gttgtcaaag tttgtcgacc ccacggatac catgcgccgg
     4261 tcgaccacga gcatcttgca gtgaaacata gtggggctgt attcgtggat ctccgcacca
     4321 gcttcgagta gctcacccca tacagctcgc gaagccttgc ggacgacttc cgtgtctata
     4381 tgcgtgccgg gaacaatgat gcgcacccgc acgccgcgct ccagggcaga ccggatggcc
     4441 tggcgcataa ggtcgtcggg cacgaaataa gccgcagaca ggtcaattgt gcgctccgcg
     4501 gccgtaattg acatcagata catgagaagc atgctgtcgc caccgccgct tggtgagctt
     4561 gtaaatactt gagcgcgtgc attccccacg ggctcaagtg cgggaaaata ctcttcgccc
     4621 tggagcacat gcccagtcgt cttgatccag ttgtctatga acgcagcctg catgtgcgcc
     4681 acagcgggac cttccaggcg atagtgagaa tcccgccaat gttctgacga ctctgcgttg
     4741 cctttccaga tatcggcaat gccaacgcct ccggtgaacc cgacatgacc atcaacgatc
     4801 aacaacttgc ggtgagtgcg attgttcatt cggccaaggt tgtaccagcg aagtggatga
     4861 aagcgttcca ctttgacgcc tgcctcgatc atcgtctcaa gaatcgaatc gtccatcttc
     4921 tggccgccca cccagtcgat aagcacgtgc acgtcaacac ctgcggctgc gcgctccgcc
     4981 aaagcggcgg cgaactcgcg cccgatctcc cccgaccagt aaatgtaggt ctcgaaatta
     5041 atggtctgac gggcctcgcg cacagccgct agcatcgcag gaaagatttc gtcgccgttc
     5101 tcgagattgg tcacccggtt accctcgatg ataggcgggc cgagcaggat gctgagttcg
     5161 cgaaggaact gagggtcgtc aattccgtag cgatgttcga gcttgcgttc gatcttcttc
     5221 tcgccgccga caaagtttag cgcgaacacg gtcaccagca ccgcggtcag ggcgcctagt
     5281 attgtggaaa caatcatggg cttacggctc cggcgcatgg gacgaacagt ctggagagta
     5341 agtcaatttc caacggctcg cttgttcaat ccggctctcg ccaagctgcc aaagtgtccc
     5401 tgatcggttc gggagcgtcc tcccgagaca ggatgcctac gtttggcctt cgatatccac
     5461 ccgtacagct agattgtaat gcggatctga gtcctcaaga tagagtaagt gtgtctggcg
     5521 ccatcctccg tccgactgcc gcggattgac tcgagcgccg cttatatttt gactctccga
     5581 cagattcgaa ctcatagcct tcgaccaacg cctcgcggac aatacccctc ttccctcttc
     5641 cctcttgccc gcgctatgct gtcggaccag ggagcgagcg cttcatgcgc gcacgatcgc
     5701 ttgcgaagcg aatgaggcgg ccggcgctcg cgggccgctg gaacaggtgc ttggctgctc
     5761 gccaataatg ccggtcggcg acataacgct tgagttgctc aaaccagaac agaagcgacc
     5821 atcgctgctc gtgctcgtcg agcgcaccga tgagttcggc tgaaccaggg tgagggacag
     5881 atgcgcgaag acgctggctg ccggataccg cgtgccgcgc catgcgcggc agcagcatct
     5941 cggtccggct cgcggatgca ggcgtctgcc ggtagacata gtggctctcc gggtacagta
     6001 tgaatcgcgc gccgcagaac agggcctgta cgtagagatg gatgtcctcc gcaaacctca
     6061 ggtcttccgc ataccgcagg ttgcactcgt cgaggaagcg cctacgcatc agaggcttga
     6121 gatatcccag gctgcaatgg ccgccggccg ggatgtcact ctttatgagg gcctccgccg
     6181 acaatagttc cacgtcgtgt ccgttgcggc gccacgtcat gagctccgcc ggagtaccgg
     6241 gcggtctcgc gccttgaaat agaatgccgt cggcaatcag gtcggcatgc tctcgctcgc
     6301 cagcctcgac cagtaagcgc aatcgatccg ggtttatctc atcatcggca tcgacgggcg
     6361 caatccatat gccgcgcgcc ctctccagcg ccaggttacg agctgccgcc tgccctcgat
     6421 tgcccgcctg gcgcaacagt tgcacgcgcg ggtcgcgggc cgcgaagcgc tcggctacgc
     6481 gcgcgctatc atctacggat ccgtcatcga ttatgagcac ctcgaagttc ggcaacgttt
     6541 gtgccagcac cgattcgacg gcggcgggca ggaatcgcgc gccattgtag acaggcatga
     6601 tgacagagac ggccagttcc tcagcatgct gcggcacaga caccgtactc accctcgatt
     6661 gcgccaggct gatcaagacc taaaccgtca gctcgaacat ccgcccgtcg tgatcgcgga
     6721 gctcgacacc gaaaaagtgg gcgatgcttg gggcgagatc agcggttttg atcgcgggcg
     6781 cttctccaca gaaatccgca ccgtgcccag cgatgatgca gatgccgtcc ggtgtgtggt
     6841 cgcccgtgcg attgtcgagg tgctcgcgat acagttcacc gatcgtgtcg gaccggaccc
     6901 tttcgatcgg cgcgttgcga ttccaagtaa cgagcaagtc cggcagcctc tcgaggtacg
     6961 ggccagaata ctccttgcgc gtcacgacga tgtctttgat cagccgctcg ccagtgtcgg
     7021 cgttgacgac cttcatcagc tctgtcgtga ggaagcgcag gagcgcgtcc gtttctcccg
     7081 gttctacggt accgctgccc tcgcggccct tgacgttgag ccggacgcca cccgacgcgt
     7141 tgttcgcgta cacctcgaag aaccgtcggt tcgcgtgatc gccgaggaac ttgggcgggt
     7201 gtagcgcacc gcgaaacggc cgccgcagaa gcttcaggcg accgcgcatc tcgatgggta
     7261 cacggtgcca aagcgcgcgc agcctggctt tgcgcgagtg tggcgctacg tgacctgtgc
     7321 ccttgtcgag ccgcagcagg atgcgatcga gcaagccagt gccgctgaat gagggtccca
     7381 tgccatggct gccgaacagc atgaccgtcg tttgtgcatc gatcgctgcg agaatcgtct
     7441 cgaggctcct gtcgaacgcg cggtaacaat ctcgcagcgg ctccccgagc tctgcctcca
     7501 cctttgccga gtaacgcggg tgacgcggct cgttgatatg ccacaggtga tgtccggcgc
     7561 aatgcaggtc cgagctcgta accagtgcga cgtcccagtc ggtgcttttc aggaagtggt
     7621 gcgcaagctc tgccttacct tcaattcgcc gcaggtagat gtcgcgaaac cgccggtaat
     7681 cgtcgaggct ctccggcgac atgacgtcgc acatgacccc ccgcgccgga tcgggaccga
     7741 cgagcgccag gacctcggcg gcgacttccg gcgggtgcgt cttgaactca aacgtattgc
     7801 cgttcgccgg cacgtgcgtg ccccagtcca tgatcgtgac gccgttgatc tgcggatcga
     7861 cgagggtata cggggggtcg atcagcaggc aactcagtcc ggcgtccgag agctgccgcc
     7921 atacggttgg cgcagtctcc tcaggaccgt accagcgttc cacgtacgtc tgcgaattga
     7981 aattccgccg gccgtcatac atgggctggc ggcccggcag cagacccgtt tggaaaaccg
     8041 gccagacggc gccagcctcc atcccgcgtg gattctcgac gcgtcgcgta ctaccggccg
     8101 ccatcaggcg gctgatgtgg gtcaggtcgc ctgtcgcgca ccacttgcgg aacagctccg
     8161 ggctgccggc gtccactccc acgatgacga gcttccgctg cccgttgcct cccgtccgtg
     8221 gcccggcccg ctccgtatcg ctacgcctgg caccaggtaa cgccgtatct tgatgattcg
     8281 gcatattctt gggtaaccca cgctctgttg attcaaatgc cccaagagcc tgcaaggggt
     8341 ggcctttcag gccttgaggc ctatccaagg ttagttcgcg aagtcgaaga accagtcgtc
     8401 cacctcgctg cccgtcagca ggtccaccag tacgacgcgg aattggcttt gtgggctaat
     8461 gatgtcgtcg tcgcgggtga gcaaggccag ggacggcgtt gatacgccct gtcccggcgc
     8521 caggtagatc acgtcctcga tgagctcgaa gggttgcagg aacacattgt gcaacaacgc
     8581 gccggtggcg aggtcccgcg tctcggcttt gaccttgccg acatcattgc ggcccgcgag
     8641 agtgaactgc ggcgacaggt tggcatcgac atccggcgag tgctcgaaat cgtccggcgc
     8701 gaaggcggcg ttgaacggca gcttcgacag cacacctaca tccgggtcga caacctcgat
     8761 atccagccat ccttgttctg cgttttgcct gagcaccgcg atgcgggatg cgccaccgtc
     8821 ttcgtattgc aagccccgca gcggcgtcat ttccgttccg agccagaggt tcgcctcgag
     8881 cgacccgtcc ggattgcgga tctcgagctt gtcgggcgat gtgccggacg tatccacggc
     8941 tagtacggca taacgcagcc cgctcgcgga cggcagcgcg tacaggtcga gcggtgtgaa
     9001 ggccgggtta tacgcgatcg tgccgaggcc gaccccggtg gcggcgtcaa aaatgctcgc
     9061 acgcacgtag ttcttggtcg agtgtttgtg caggacagca agcacggacg tcgcaccttc
     9121 gttggcaatg acggcggcga tcggctcgaa gtcgtcgtca agttcgaggc gcaggatttg
     9181 atcaccgctc tcgttgtcga cgatctcgac gacgggcggt accttgctca gcacgacgag
     9241 ctcgtcgtga ccgttgccgt ccatatcctc cgctgcgaaa aaatccaccg gtcgcaaacc
     9301 gtcgccgaag aacacggtat tgccggtgtc gctgcccggc acctgcaccc gcgccgccac
     9361 cggcccggcg ccgtaatgat ggtcgagcac ggcgagccgt tccgcggatc cgggaatcga
     9421 gccatcgagc cgagccagtg caggcttcag gagcggcgcc ccggaaagcc gcccggtgat
     9481 gcctctgtcg gtttcgaggt cgtacccgcc aatgtagacc tcatcctcca gcacggacac
     9541 cgcgttcggc acggcagcga gcgacggaaa gtcccagcgc gaaatttcct ctc
//
LOCUS       DASUOM010000297         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1204462,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000297 DASUOM010000000
VERSION     DASUOM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204462"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFY27_06305"
     CDS             <1..464
                     /locus_tag="VFY27_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765160.1"
                     /translation="ICSVSYCISPAFCDYFKHWKHNGYWFFDSPRVIQEVARAESVDL
                     SDHKVFYYEVYEQQFHEVAKRWQRFEADKHLETNIQPPGQKELLGFDVVNFSVQTSPE
                     CSPLSCNSLSESIAVNQHCLLASFAEARQLLEAGAFDDSEPGPYRVFAVYT"
     gene            471..1037
                     /locus_tag="VFY27_06310"
     CDS             471..1037
                     /locus_tag="VFY27_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765161.1"
                     /translation="MVPNRRTLIFAACVPVGIAAIGCLVSLYISLGPFWPLAIMAGSA
                     VSVIVTTRVWRNTRLSVVASAEIAGAYILVQVALLALTQRESREIFLMQSSAARTSGL
                     PAEVRLAFREEPGSFIGIRSPELAAYLDTSSASEVPAEFTVSRDLGCVRGFTPVRVGP
                     VMLRPTTTGYFGSRNPARLPFMEPWWCP"
     gene            1266..1865
                     /locus_tag="VFY27_06315"
     CDS             1266..1865
                     /locus_tag="VFY27_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765162.1"
                     /translation="MHYSVDMTKSLSSHGRRSIALVAAIFVSASVGCGDFVSPLGRTL
                     PPELLLRATAEGVDAGLSIECAVNGHLELEPEVIDDGNHRVQHGSAGGEALRALQRPD
                     GSGVQFWGDYQMATLEVHRIGADSIEIRDVGIYSDVVPSRFWSEFLVFRGHATPNEWS
                     AGIIARGAWTCYPMDTPPSSGEYHDPVGTIVGTWTLSTH"
     gene            2123..>2323
                     /locus_tag="VFY27_06320"
     CDS             2123..>2323
                     /locus_tag="VFY27_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765163.1"
                     /translation="MSFTLRAYGPKMPQHSGHWLPNPAQQTARAISSFQDLAGRVPMS
                     GWYDGIVLDPSAIALLDAVPDKE"
BASE COUNT          438 a          688 c          693 g          504 t
ORIGIN      
        1 atatctgttc cgtcagctac tgcatatcgc cggcattttg cgattatttc aagcactgga
       61 agcacaatgg atactggttc ttcgactcac ctcgcgtcat acaggaagtc gcgcgagccg
      121 agtccgtcga cctatcggat cacaaggtgt tctactacga ggtctacgaa cagcaattcc
      181 atgaagttgc caaacgatgg cagagatttg aggcagacaa gcacttggag acgaatatcc
      241 agcctccagg gcagaaagag cttctggggt ttgatgtagt caacttcagc gtacagacaa
      301 gtcctgagtg ttcgcccctt tcctgcaaca gcctttcgga atccattgcc gtgaaccagc
      361 actgccttct cgcctctttc gctgaggcaa gacagctcct ggaggccggt gcatttgacg
      421 attccgaacc aggtccatac cgagtatttg ccgtctacac ctagatcgcg ttggttccga
      481 atcgccggac actgatattc gctgcctgcg tgccggtcgg catcgcagcg atcggctgcc
      541 tggtgtccct ctacatctcg ctcggaccgt tctggccgtt ggcgatcatg gccggtagcg
      601 cagtctctgt catcgtgacc acacgtgtct ggcgcaacac gagactatcg gtggtggcgt
      661 cggcggaaat cgctggagcg tacattctcg tccaggtcgc gctcctcgct cttacgcaac
      721 gtgaatcccg tgaaattttc ctcatgcagt cgagcgcggc gcggacatcc ggtcttcctg
      781 ccgaagttcg gttggcattt cgagaagagc cgggcagctt catcggcatc cgttcgcctg
      841 agctcgcggc gtatctcgac acgagctccg cttccgaagt acctgcggag tttaccgttt
      901 cccgcgatct tggttgcgtc cgtggcttta cgccggtgcg ggtcgggcca gtgatgttgc
      961 ggccaacgac gacgggatac ttcggctcga ggaacccggc ccgcctgccc ttcatggagc
     1021 catggtggtg tccctagcgg tcaggagcgc aagcgcgcta ccgaccccaa atgcgacacc
     1081 gggcaacatc gcgttccacc tcgaatgcaa gccgtgctgt tatgacagcg gcgatgcctg
     1141 tgagcgcgtg gacgatgcgt ccaacagcgc caacaggctt ttcgaggact cttcgtcgcc
     1201 gcgacaggtg aggcattatc tcaggacaga cgtgtcgtcc catgtttgac gccctgagga
     1261 ggaaaatgca ttacagtgtc gatatgacca agagcttgtc ttcacacggg cgtcgatcga
     1321 ttgcgctggt ggctgccatt ttcgtctccg cgagcgtcgg atgcggtgat ttcgtgagtc
     1381 ccttaggtcg tacgctgcca cccgaattat tgcttcgtgc gacggctgaa ggtgtcgatg
     1441 ccgggctgag catcgaatgc gcggttaacg gacacctcga gctggaaccg gaagtcatcg
     1501 acgatgggaa tcatcgcgta cagcacggat cggctggtgg tgaggccctg agggctctcc
     1561 aacgtccgga tggatcaggg gtacagtttt ggggcgatta ccagatggcc actctggagg
     1621 ttcatcgcat cggcgcggac agcatcgaga tccgggacgt cgggatttac agcgacgtgg
     1681 tcccaagtcg attctggagc gagttcctcg tctttcgcgg ccacgcgacg ccgaacgaat
     1741 ggagcgccgg catcattgct cgcggcgcat ggacttgcta cccgatggac actcctcctt
     1801 ccagcgggga ataccacgat ccggtgggca ccatcgtggg aacctggact ctttcgacgc
     1861 actagacgca gcgcgtctta cacgggtcat tcaagtgcat cggcggccgc gtacaagtat
     1921 cacgctgatc atgtcaaagc gaggattcgt tgcgcgatcg cggctaaggc catagtggac
     1981 accgaggagg agctcgtctc gccgcgattg ccggccactg tgcgaaagca tcgccatctg
     2041 ctgagcgcgg accatctcct ggttctcgat ggacctccgc acgtttccgg agagccgacg
     2101 gtcgtctggc gcccatggga tagtgtcgtt cacgctccgt gcgtacggcc cgaagatgcc
     2161 gcagcacagc ggacattggc tgccgaatcc cgctcagcag acggcgcgcg cgatctcgtc
     2221 gtttcaggat ctcgccggac gcgtgcccat gtccggctgg tacgatggca tcgtgctgga
     2281 cccttccgcg atcgctctct tggacgccgt tccggacaag gaa
//
LOCUS       DASUOM010000298         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_981143,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000298 DASUOM010000000
VERSION     DASUOM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981143"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..702)
                     /locus_tag="VFY27_06325"
     CDS             complement(4..702)
                     /locus_tag="VFY27_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765164.1"
                     /translation="MANRFNVRRPSRFACGTLLAMLICSSSYAEADRRPANDDVQRFE
                     QYAAGAIYKVFPLDRQEFYLTVADSSKRAFAFFNPTSDGLVAIKCGQAAHFRIVRHAN
                     EVADLSIGECGKERFSIEEAVRSAPTRLKQALGSLSANAPSATEEQLRRMGFFYEKRL
                     LADGYEQHYFPVLIVSHGLIFLPTIVVVKGQATWVVQANTTNLCSQDEHHPLCSDVAE
                     RLSRIAANLVSGKR"
     gene            complement(681..2996)
                     /locus_tag="VFY27_06330"
     CDS             complement(681..2996)
                     /locus_tag="VFY27_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765165.1"
                     /translation="MIAALLAASGLHAHADSCIWYADDDSIRQVQTSTNQTTRVVPFR
                     APHRLIMNAEDCGVWVLDKLERTLLRFNAEGAFERLIHVADLGVGLDVVDRLHVNPYD
                     GSLWITDNWRVAHVSAGGELLGNFAAPGEVRRMAVALDQTLWLLGKKDLWRFDAKGTL
                     LASYPLGRHLIGDARYFAVDSLAGLIWLVDDNNFARFKLADPMEEPPLQIQLQHQVTG
                     LALDPLAGNVWVAQSDSLLAFSQSGAVVHQLNLPALSIFNAEKLGFDPVSRSVWVGAQ
                     RSLSRFTDAGQFVTRFVARDGDEALGVPAFRVQPTLTLVRPPQDALANNPQPEFRLGY
                     GAECNAASCGFPGAYFRSYELSATLNNQQVGSFFIFDPNSVESSFTPSSRLPEGENSF
                     SAQVTDRFGHRSNTISNMVTVDTIAPQFLALSPADGAVLQVPQALLQGTVDDPLATVV
                     LENLGLTQTGASFSFPVTLQPGPNIFRISAIDRAGNSVTAQRTLNLASLSVSITSPVS
                     GATVSGSSVLVTGTIQGPPNTGVTVNGVVAAIIADRFYARVPIQTGPNVLTVIAKTQD
                     GFTVQQALSVTGSGPVAIEISATPQSGLAPFKAKFKFNLSVGTSVSTILADLDGNGSI
                     DFTTTDLTTPTEFTYPQPGVFQATFIIRHTQGNTTVQTLQVVVQDPNQLDAQLKAVWS
                     GFVSKLVARDKAAAMQHFSASAGGRYGPVFDALLPDLPQIAASFSPPLRAELGSAYAE
                     YAVVRVIGGVKRVYMIQFVVGADGVWRIDSM"
BASE COUNT          635 a          937 c          984 g          601 t
ORIGIN      
        1 ggttcacctc ttgccgctaa cgagattggc ggcgatccga ctcaagcgct ccgcgacatc
       61 gctgcatagc ggatggtgtt cgtcttgaga gcataggttc gtggtattgg cctgcaccac
      121 ccaggttgcc tgccctttga cgacaacgat cgtcgggaga aagatcaggc catggctgac
      181 gatcagaacc gggaagtagt gttgctcgta accatccgcc aacagacgct tctcataaaa
      241 gaagcccatg cgtcgaagct gctcctcagt agcactgggc gcgttggcgc ttagtgagcc
      301 gagggcttgc ttgagcctgg tgggagcgct tctcaccgcc tcttcgatac tgaaccgctc
      361 ctttccgcat tcacctatgc ttaggtccgc tacctcgttc gcatgcctaa caatccgaaa
      421 gtgcgcggcc tggccgcact tgatggccac gagcccgtcc gacgtcggat tgaagaatgc
      481 aaaggctctt ttcgaactgt ctgccacagt cagatagaac tcttgtcggt ccagtggaaa
      541 gactttatag atcgcgccgg cggcgtattg ctcgaatctt tggacgtcat cgttagccgg
      601 acgtcgatcc gcttcggcat acgaggacga gcaaatcagc atcgcgagca atgtgccgca
      661 agcaaacctg ctcggacgcc ttacattgaa tcgattcgcc atacgccatc ggctcctacg
      721 acaaactgga tcatgtacac gcgcttcacg ccgccgatca ccctgacgac cgcgtattcg
      781 gcgtatgcac tgccgagctc cgcgcgcagc ggcggcgaga acgacgcagc gatctgcggc
      841 agatcgggca gcagcgcatc aaaaaccggc ccgtaccgtc cgccggcaga ggcggaaaag
      901 tgctgcatgg ccgccgcctt gtcgcgtgca acgagcttgc tcacgaagcc gctccatacc
      961 gctttcaact gcgcgtcgag ctggttcgga tcctggacca cgacctgcaa ggtctgcacg
     1021 gtcgtgtttc cctgggtgtg cctaatgatg aatgtcgcct ggaaaacgcc cggctgcggg
     1081 taggtgaact cagtgggcgt ggtcaggtcc gtggtcgtga agtcgatgga gccatttcca
     1141 tccaggtccg ccaggatggt tgacaccgaa gtgccgacgc tcaggttgaa cttaaacttc
     1201 gctttgaagg gcgcaagtcc ggactgtggc gttgcgctga tctcgatggc caccgggccg
     1261 ctgcccgtta cggagagggc ctgctgtaca gtgaacccgt cctgggtctt cgcgatgacg
     1321 gtgaggacgt tcgggccagt ctggatcggc acgcgggcgt agaagcgatc ggcgatgatc
     1381 gctgccacca cgccgttaac cgtaacgccg gtgttgggcg gtccctggat tgttcccgtc
     1441 accagcacgc tggagccgga aaccgtcgca cctgaaaccg gtgacgtgat gctgacgctc
     1501 agcgaggcga ggttaagggt gcgctgcgcc gtgacggaat tgccagcgcg gtcgatcgcc
     1561 gagatcctga agatgttcgg cccgggctga agggtgacgg ggaagctgaa gctcgcgccg
     1621 gtttgcgtca ggcccaggtt ctccagcacc accgtggcga gcgggtcgtc caccgtgccc
     1681 tgcagaagcg cctgcgggac ctgcaagacg gcgccgtcgg ccgggctgag cgcaaggaac
     1741 tgcggcgcga tcgtgtcgac cgtgaccatg ttcgagatgg tgttggagcg gtgtccgaag
     1801 cggtctgtca cctgggcgct aaagctgttc tctccttcgg gcagccggct ggacggagta
     1861 aaggacgatt cgacgctatt tggatcgaag atgaaaaagc tgccgacttg ctgattgttc
     1921 agcgttgcgg acaactcata acttctgaaa taggcgccgg ggaatccgca agaagccgca
     1981 ttacattccg cgccatagcc aagccggaac tcgggctgcg gattgttagc gagtgcatcc
     2041 tgcggcgggc ggacgagcgt caatgtgggc tgaaccctga atgcgggcac gccaagcgct
     2101 tcgtccccgt cgcgagcgac aaagcgggtg acgaactgcc cggcatccgt gaagcggctc
     2161 aacgaccgct gcgcacccac ccaaacggag cggctgaccg gatcaaagcc gagcttctcc
     2221 gcgttgaaaa tactgagcgc tggaagattg agctggtgaa cgaccgcacc gctctgagag
     2281 aaagcaagca gcgaatcgct ttgcgcgacc cagacattcc cagccagggg atcgagggcg
     2341 aggccggtaa cttgatgctg cagctggatc tgcagcggcg gttcctccat tggatcggcg
     2401 agcttgaatc gggcgaaatt gttgtcgtcg acgagccaga tcagtccagc gaggctgtcg
     2461 actgcgaaat agcgcgcgtc gccgatgaga tggcgcccaa gcgggtagct cgccagcagc
     2521 gtccccttcg catcgaagcg ccaaaggtct ttcttgccga gcagccagag cgtctggtcg
     2581 agcgcaactg ccatgcgccg cacctcgccg ggggctgcga aattacccag gagctcgccg
     2641 cccgccgaga catgcgcgac gcgccagttg tcagtgatcc aaaggctgcc gtcgtaagga
     2701 ttgacgtgca gcctgtcgac cacgtctaac cccacaccca ggtcggcaac atggatcagg
     2761 cgctcgaacg ccccctcggc attaaagcgc aggagcgtac gctccagttt gtcgagaacc
     2821 caaacgccac agtcctcggc gttcatgatc agccgatgag gagcgcggaa tgggactacg
     2881 cgggttgtct gattggtgct tgtctgtacc tggcggatgg agtcgtcatc cgcgtaccaa
     2941 atgcacgagt cggcatgcgc gtgcaagccg ctggccgcga gcagcgcggc aatcaatact
     3001 ctcagacccc ctcccccggc ttgcgactta ttgttcgacc atcgattgtt ggcggcttaa
     3061 gaaccgcgtg tcaatgatgt tgataacgcg gtggacgctg agataagaaa tcgagacgag
     3121 gcgtctagct aggctactca gccagcagcg ctttcag
//
LOCUS       DASUOM010000299         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1507727,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000299 DASUOM010000000
VERSION     DASUOM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507727"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VFY27_06335"
     CDS             <1..1229
                     /locus_tag="VFY27_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5765166.1"
                     /translation="PRILMEKVQPQLQAQITDNVEESLFYKPIKEMPEEFSEQDRERL
                     AAAYRDAISQKIVPAYERMYNFIGDEYLAAARDSVGMVGLPNGEEWYAFLVRQRTTTD
                     LSPQGIHQIGLDEVARIHGEMQGVMDEVGFEGDLKDFFEYLNTDDQFYYDEPEQLIQG
                     YRDMSDHIESLTPKLFSVFPKTDFEVRAVEPFREKSASGGQYRRGTPDGSRAGVFFAN
                     TYDIKARPKWAMESLFLHEAIPGHHFQISIQQEIEGLPRFRRFGGYTAFTEGWGLYAE
                     SLGKELGVYTDPYQYFGALNAELWRAIRLVVDTGLHFKGWSRQDVLDYMYTNSAVKEA
                     RAVSEAERYMAIPGQALSYKIGQLKIRELRERAEQALGDRFDIKAFHTEVLTDGSLPL
                     SMLDAKIDRWIEAQRS"
     gene            1400..2161
                     /locus_tag="VFY27_06340"
     CDS             1400..2161
                     /locus_tag="VFY27_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5765167.1"
                     /translation="MAVPAPFTNHLLDGLSCKDRNKLLNAGETVQLHFGEVLCEPRQA
                     FGYAFFPLTGFISLLSTLHDRQPLELGLIGNEGMLGATLALGLDAAPLRAVVHGPGTA
                     LRLRVSELRRQFLSSPSLLKMLHRYQYVLMAQLSTSAACAHFHAVEVRLATFLLMAHD
                     RAQSDHFKLTHESLSGMLGARRSGVTVAAGVLQRKLLIRYTRGEISILDRAGLEDACC
                     ECYSATIHVYAQLLGGAAARPTAKLPESIRTLPYR"
     gene            2203..2934
                     /locus_tag="VFY27_06345"
     CDS             2203..2934
                     /locus_tag="VFY27_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5765168.1"
                     /translation="MAETTAAGRQPAGPKLNHLLAALPAADYERISPELEPIELPLGM
                     VVYESGGTLGYVYFPTDSIISLLYVMEDGASAEIAVTGYEGLVGIALFMGGETTPSRA
                     VVQSAGSGYRLKAALLKKEFDIGGALQHLALRYTQALITQMAQTAVCNRHHSVEQQLC
                     RWLLLSLDRLSSNQLSMTQDLIANMLGVRREGVTEAAGRLQKAGLIRYSRGHIVVLDR
                     PKLEARVCECYAVVKKEYDRLLETG"
     gene            complement(3164..>4074)
                     /locus_tag="VFY27_06350"
     CDS             complement(3164..>4074)
                     /locus_tag="VFY27_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5765169.1"
                     /translation="MYNHRNKYAKIIVSTAALAVLGACATPLMAPEGSESARNKLTQL
                     QSDAQLSSRAQVEIKAAETAVAAAEVPNKDQERAKHLVLLADHKVDIARARAQSRLYE
                     DERQALSEQAELARLQARTREADKARLDATAARKDAMSARADATAARLEADESRETTM
                     AARLESEDLQRQIEELNARETDRGLVVTLGDLLFETGKSDLKGGAAANLNKLALFLGE
                     YPDRSVLIEGHTDNVGSDETNVNLSQRRANSVQSYLVGQGIAAARLSTSGLGEGSPVA
                     GNDTATGRQQNRRVEVIISNATTASR"
BASE COUNT          765 a         1243 c         1259 g          807 t
ORIGIN      
        1 agccgcgcat cctgatggag aaagtacagc cacagctcca ggcgcaaatc acggacaacg
       61 tggaagaaag cctcttctac aagccgataa aggagatgcc ggaagaattc agcgagcagg
      121 accgggaacg gctggccgcg gcgtatcgcg atgcgatatc gcaaaaaatc gtgcccgcct
      181 acgagcgcat gtacaacttc atcggcgacg agtacctggc ggcggcacgg gacagcgtcg
      241 gcatggtggg gctgccgaac ggcgaggagt ggtacgcgtt cctggtgcgg cagcgcacga
      301 cgacggacct gtcgccgcag ggaatacacc agatcgggct tgacgaggtg gcgcgcatcc
      361 acggcgaaat gcagggagtc atggatgagg tggggttcga gggcgacctg aaggattttt
      421 tcgaatacct gaacacggac gatcagttct actacgacga gccggaacag ctgatccagg
      481 gctatcgcga catgtccgac cacatcgaga gcctgacgcc gaaactgttc agcgtgtttc
      541 cgaagaccga cttcgaagtt cgcgcggtcg agccgtttcg cgaaaaatcc gcaagcggcg
      601 ggcagtaccg tcgcggcacc cccgacggct cgcgtgccgg tgttttcttc gccaacacct
      661 acgacataaa ggcccggccg aaatgggcga tggaatcgct gttcctgcac gaggccattc
      721 cgggacacca tttccagatt tccatccagc aggagatcga ggggctgccc cggttccgcc
      781 gtttcggcgg ctacacagcg tttacggaag gctgggggct ctatgccgaa tcactgggca
      841 aagagctcgg cgtctacacg gatccctacc agtatttcgg cgcgctgaat gcggagctgt
      901 ggcgggcgat ccggctggtc gtcgataccg ggctgcactt caagggatgg agccgtcagg
      961 acgtgctgga ctacatgtac acgaactcgg ccgtgaagga agcacgtgcc gtgagcgaag
     1021 ccgaacgcta catggcgatt ccggggcagg cgctgtcgta caagatcggg cagctgaaga
     1081 tccgcgaact gcgtgagcgg gcggaacagg cactcggcga tcgcttcgat attaaggcct
     1141 tccacactga agtgctgact gacggctcgc tgccgctcag catgctggac gcgaagatcg
     1201 accgctggat agaagcgcag cgcagctgac ctgggaggcc gggcttcccg ccctgcctgc
     1261 aggagcagga tgcgccgcac tatgtatggc agcgaacaga ccggctgcag tacgtgagcg
     1321 acactgcgcg gacgagactg cttggcacct ttctcgtccc acctgtcggg cttgcttcgt
     1381 gctgcgcgga ccttaaacct tggcagtacc cgccccattc accaatcacc tgctcgacgg
     1441 actgtcgtgc aaggaccgca acaagctgct gaatgctggt gaaacagttc aactccactt
     1501 cggggaggtg ctgtgcgagc cgcggcaggc ctttggctac gcgttctttc cgctcaccgg
     1561 ctttatttcc ctgctatcga cgctgcatga ccgtcaaccg ctcgaactcg gcctgatcgg
     1621 aaatgaaggt atgcttggcg caacactcgc tctcggcctt gatgcggcgc cgcttcgcgc
     1681 ggtggtgcat ggccccggca ctgcattgcg gcttcgcgtt tcggaacttc ggcggcagtt
     1741 tctttcaagc ccgtccctcc tgaagatgct tcaccgttac cagtacgtgc tgatggccca
     1801 gttgtccacg tcggctgcct gcgcccattt ccatgcagta gaggtacggc tcgcaacctt
     1861 cctgctgatg gcgcacgatc gcgcgcaatc cgaccatttc aagctgacac atgagtccct
     1921 gtccggcatg ctcggtgctc gcaggagcgg cgtcacggtc gctgctggcg tgctgcagcg
     1981 aaaattgttg atccgatata cgcgcggcga gatcagcata ctggatcgtg ccggcctcga
     2041 agatgcctgc tgcgagtgct actcggcgac gattcacgtt tatgcacagt tacttggcgg
     2101 agcggcagcg aggcccaccg cgaaactacc agagagcatc cgtacgcttc cgtacagata
     2161 gggtacactg acccggcacc cgatcctgag tagtgttgct tcatggcgga aacgactgcc
     2221 gcaggccgac agcctgcagg ccctaaactg aaccacctgc tggcggccct tccagccgcg
     2281 gactacgagc gcatttcgcc ggaactggag ccgatcgagt tgcccctggg catggttgta
     2341 tacgagtccg gcggcactct cggctacgtg tatttcccca ccgacagcat catttccctg
     2401 ctttatgtaa tggaggacgg ggcatcggcg gagatcgccg taaccggtta cgaaggcctc
     2461 gttggcatcg ccctgttcat gggcggtgaa accaccccca gccgggcggt ggtgcaaagc
     2521 gcgggatcag ggtatcggct gaaagcagca ttactgaaga aggagttcga catcggcggc
     2581 gctcttcagc atctcgcgct gcgctacacg caggcactga tcacgcagat ggcgcagacg
     2641 gctgtatgca accgtcacca ttctgtggag cagcaacttt gtcgatggct gctgttgagc
     2701 ctcgaccgat tgtcctcgaa tcaattgtcg atgacccagg acctgatcgc caatatgctg
     2761 ggcgtgcgcc gcgagggcgt gacggaggcg gccgggcggc tgcagaaggc ggggttgatc
     2821 cggtacagcc gcggccacat cgtggttctg gaccggccaa agctcgaagc tcgagtgtgc
     2881 gagtgctatg cggttgtcaa gaaagaatac gatcgcctac tcgagacggg ctagacccac
     2941 cgacttcggc cttcgctcgc ctgccgatca ctgttcgacc gatactgtcc ggacggatag
     3001 cgttcctgtg tgtcgtcagg catcggctgc ctgagcgtcc gtccaatagc caggtgagcg
     3061 ttcagcgcac caatctttcc ggtacttcaa aaaaaagccg ctccgtgagg agcgggttcg
     3121 ataatgccgc cgccgcgatc agccgctcag gttgagcgga aaatcagcgg gaggcagtag
     3181 ttgcgttgga gatgatgacc tcgacacggc ggttctgctg ccggccggtg gcggtatcgt
     3241 tgccggcgac gggggaaccc tctcccagtc cggacgtcga cagccgcgcc gctgctatgc
     3301 cctggccaac caggtagctc tggacggaat tggcgcgacg ctgcgacagg ttgacgtttg
     3361 tctcgtcact gccgacgttg tccgtgtgac cttctatcag gaccgagcga tccggatatt
     3421 cgccgaggaa cagcgccagc ttgttgaggt tggccgcggc accgcccttc aggtccgatt
     3481 tgcccgtttc gaacagcaga tcaccgagcg tcactaccag gccgcggtct gtctcacgcg
     3541 cgttcaactc ctcgatctgc cgctgcagat cctcggactc cagcctcgct gccattgtag
     3601 tctctcgcga ttcatccgcc tccagtcgag cggcggttgc atcggcccgc gcagacatgg
     3661 cgtccttgcg tgccgccgtt gcgtccaacc tcgccttgtc ggcttccctg gtgcgcgcct
     3721 gcagtcgcgc gagttcggct tgctcgctca aagcctggcg ttcatcctcg tacagacggc
     3781 tctgcgcgcg ggcacgtgcg atatccacct tgtgatccgc cagcagtacc aggtgtttcg
     3841 cgcgctcctg atccttgttc ggcacctcgg cagcagctac tgccgtttcg gcagccttga
     3901 tctcgacctg cgcccggctc gagagttgcg catccgactg gagttgcgtc aacttgtttc
     3961 gcgcggactc cgagccttcc ggggccatca gcggcgtggc acatgctcct aacacggcga
     4021 gggctgctgt gctgacgatg attttggcgt acttgttcct atggttgtac atta
//
LOCUS       DASUOM010000300         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1505784,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000300 DASUOM010000000
VERSION     DASUOM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505784"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFY27_06355"
     CDS             complement(<1..661)
                     /locus_tag="VFY27_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765170.1"
                     /translation="MLKQFVLLGLAAAAATAATASEHSYQLLQPALDESGLPLTRAHE
                     GIAYPVTRLADDSLRGSLARLFESGFPRAMLALDQLARDIEGAPEGECSQIANQALIY
                     LSLEDGGYARKGFWFSEGDAEPQFCDLHFVDLTADADAIDDGSVEELLAHEMAHVWLR
                     RLVGELPPGASRAFHSAFAVTDYVTAFDEGFAMAMQPLAERYSHTDALAAHTWGATGP
                     DF"
     gene            complement(755..1195)
                     /locus_tag="VFY27_06360"
     CDS             complement(755..1195)
                     /locus_tag="VFY27_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX5765171.1"
                     /translation="MRRFFKLSRTTLPVVLIILSTGVSPGALAESEVIHQLRIYQLFD
                     DTREAFHERFRDHAQRIMARYDFHIVAMWESRNEDGPQFVYLLEWPDEQTMKDRWAAF
                     MEDQEWSDIKAETARIHGQFVGGTEERTLILTDYSPRRSLLRQN"
     gene            complement(1263..1976)
                     /locus_tag="VFY27_06365"
     CDS             complement(1263..1976)
                     /locus_tag="VFY27_06365"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5765172.1"
                     /translation="MPEFRRPHHRLVASALKSMDASFLVRTECWFGGGTMLAMTLGEY
                     RESRDIDFLCSSRAGFRSLRETVTDRSLGAIFPHSFDLARDVRTDRDGIRTFLKIENV
                     PVKFEIVLEARIDLEGAIDKTLGVPILQREYAIAEKFLFNADRGLDESTLSRDLVDLA
                     FIAAHAGRRPLEEGMEIAEQAYGSAIQKNLRSTLDRFKRNRGRTNSDLHSLGVEDTQT
                     LRKGIQLLGKLVQEPKETK"
     gene            complement(1969..>2383)
                     /locus_tag="VFY27_06370"
     CDS             complement(1969..>2383)
                     /locus_tag="VFY27_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765173.1"
                     /translation="NVRMSRVGGPATGKDMRGEQLRRAKRAQRERERHAGLVNVQLVL
                     PGDIADKLAVARRAADFPDLLDEALDQLLVHLPDYPQLRDLTWNRKDEYIPAKEAFQL
                     YERNWRFVEPGRLEPEERALIDRLKARFGAGVINA"
BASE COUNT          460 a          734 c          691 g          498 t
ORIGIN      
        1 caaaatccgg accggtcgct ccccaggtgt gcgccgccag cgcatcggtg tgcgaatacc
       61 gctcggcgag gggctgcatc gccatggcaa agccttcgtc gaacgccgtc acatagtcag
      121 taacggcgaa cgcgctgtga aatgcccggg aggcgccggg cggaagctct cccaccagcc
      181 tgcgcagcca gacgtgggcc atctcatgcg ccagaagctc ttccacgctg ccgtcatcga
      241 tggcatccgc atcggcggtg aggtcgacga agtgcaggtc gcagaactgc ggctcggcat
      301 ctccttcgct gaaccagaat cctttgcgcg cgtagccccc atcttcgagt gacaggtaga
      361 tgagcgcctg attcgcgatc tgcgaacact caccttccgg agcaccctcg atgtcgcggg
      421 caagttggtc caaagccagc atggcgcggg gaaacccgga ctcgaacaag cgcgctaaag
      481 aacctctcag cgagtcatcc gccagccgcg tgaccggata ggcgatgccc tcgtgtgcac
      541 gcgtcaacgg caaaccggac tcatcgagtg ccggctgtag cagttggtag gaatgctcgc
      601 tggccgtcgc ggctgtcgca gccgcggctg cgagtcccag caaaacgaac tgcttcagca
      661 tagcggctcc ggtccggccg gaatgcaggt tcattgttgt gttgatcctc ggtgaaggtc
      721 gcgcgggtgc gtggcggcgc taactccccg caggtcaatt ctgccgcagc agggagcgtc
      781 tgggcgagta gtcggtgagg atgagcgtgc gttcctcggt cccgccgacg aattgtccgt
      841 gtatcctggc ggtttccgcc ttgatgtcgg accattcctg gtcctccatg aaggcggccc
      901 accggtcctt catcgtttgc tcatccggcc actcgagcag gtacacgaat tgtggcccgt
      961 cctcattccg ggattcccac atggctacga tgtggaaatc gtatcgcgcc atgatgcgct
     1021 gcgcgtggtc gcggaaacgc tcgtggaagg cttccctcgt gtcatcgaac aactggtaga
     1081 tacgcagctg gtgaatcacc tcgctctccg ccagcgcccc cggcgagacg cctgtcgaaa
     1141 gaataatcag cacgacgggc aaagtcgtgc gactcagctt gaaaaaacgg cgcatacagc
     1201 tcctccaatt tattgctgca aggtgtaagc cggtaacctt agcagctcaa atactcgcca
     1261 ggctatttag tctcctttgg ttcctgaaca agctttccca gcaattgaat cccttttcgt
     1321 agtgtctgag tatcttctac tccgagcgaa tgaaggtctg aattcgtacg gccccggttg
     1381 cgtttgaagc gatcgagagt cgatcgaagg ttcttttgta tggcgcttcc gtatgcctgt
     1441 tcagcgatct ccatcccttc ttcaagtggc cgccgtccgg catgggccgc tatgaaggcc
     1501 aggtctacca ggtccctgga caaagtcgat tcgtcgagac cgcggtcggc attgaacagg
     1561 aacttttccg caattgcgta ttccctctgg agaatcggta cacccagggt tttgtcgatt
     1621 gccccttcaa gatcgatgcg agcttccagg acaatttcga acttgacagg tacgttctcg
     1681 attttcagga acgtccgtat gccgtcgcga tcggtgcgaa cgtcccgtgc gaggtcgaag
     1741 gaatgaggaa aaatagcacc gagagaccgg tccgtgaccg tctcgcggag tgaccggaaa
     1801 cctgccctgc tcgagcacag aaaatctata tcgcgtgatt cccgatactc ccccagggtc
     1861 atggcaagca tcgtgccgcc gccaaaccag cattcggttc ggaccagaaa gctggcatcc
     1921 atgctcttca gtgcactcgc gacgaggcga tggtgcggcc tgcgaaactc aggcattgat
     1981 cacaccggct ccaaaccttg cctttagccg gtcgatcaac gcccgttcct cgggctcgag
     2041 ccggccaggc tccacgaacc gccagttacg ctcgtagagc tggaacgctt ctttcgctgg
     2101 tatgtactcg tccttacgat tccacgttaa gtcgcgcagc tggggatagt caggtagatg
     2161 cacgagcagt tgatccaggg cctcgtccag gaggtccggg aaatcggccg cacgacgcgc
     2221 gaccgccagc ttgtcggcta tgtccccggg caacaccagc tgcacgttaa cgaggcccgc
     2281 gtggcgttcc cgttcgcgtt gcgcgcgctt ggcgcgtcgc agttgttccc cgcgcatatc
     2341 cttgcctgtc gctggcccgc cgactctgct catgcggaca ttt
//
LOCUS       DASUOM010000301         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_620852,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000301 DASUOM010000000
VERSION     DASUOM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620852"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="eno"
                     /locus_tag="VFY27_06375"
     CDS             complement(<1..284)
                     /gene="eno"
                     /locus_tag="VFY27_06375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780197.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5765174.1"
                     /translation="MIRIKDIRGREILDSRGNPTVEAEVWLMGGSHGRAGVPSGASTG
                     TREAVELRDGDPSRYLGKGVLKAVANVNGVIRKTLCAEPYPDQRAVDQRMR"
     gene            complement(404..1246)
                     /gene="kdsA"
                     /locus_tag="VFY27_06380"
     CDS             complement(404..1246)
                     /gene="kdsA"
                     /locus_tag="VFY27_06380"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019593221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX5765175.1"
                     /translation="MNLCGFEVGPDKPFFLIAGTCVVESEAMTVHTAGALGEMTAALN
                     LPFIYKSSYDKANRSSRSGFRGPGIEEGLRILSEVRRQVGVPVLTDVHEDTPLAEVAA
                     VVDVLQTPAFLCRQTNFILKVSEQGKPVNIKKGQFLSPWEMQNVVDKACSTGNSQIMV
                     CERGFSFGYNNLVSDMRSLAIMRGTGCPVVFDATHSVQLPGGRGTASGGQREFIPVLA
                     RAATAAGISGLFMETHPDPDSALSDGPNAWPLGKMKALLEVLQAIDASVKRLGFAESE
                     YGLG"
     gene            complement(1243..2904)
                     /locus_tag="VFY27_06385"
     CDS             complement(1243..2904)
                     /locus_tag="VFY27_06385"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5765176.1"
                     /translation="MTSYVFITGGVVSSLGKGITSACLGAILESRGLSISMIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGSETDLDLGHYERFIRTTTGRNSNFTTGRIYETVIAKE
                     RRGDYLGATVQVIPHITDEIKESIRRGAGNADVCLVEIGGTVGDIESLPFLEAIRQMG
                     VELGHDRVVYVHLTLVPYIRTSSEIKTKPTQHSVKELRSIGIQPDILVCRSEQQLPQE
                     QRRKIALFTNVPEKAVISATDVDDLYKIPSMMQKEGLDEIVASQLRLDLPPADLSEWD
                     AVVAAKQNPDGEVNIAMVGKYVDLRDSYISLSEALLHGGIHTRTRVGIHYVESQDIEV
                     KGVGCLEGMDGIVVPGGFGDRGIEGKIQAVRFARENGVPYLGICLGMQVAVIEFARNV
                     ASLSGAQSTEFDKSTPHPVIALITEWQTSAGSLEERDEDSDLGGTMRLGAQRVDLAKG
                     SLAAAIYGKEQIWERHRHRYEVNNNYRERLEKAGMRFSGLSVDDLVEMIELPDHPWFI
                     GSQFHPEFTSNPRDGHPLFTGFILAARKRHSGDLPRTVEARAAEA"
BASE COUNT          615 a         1047 c          970 g          664 t
ORIGIN      
        1 cgcatgcgct ggtctacggc gcgctggtcg ggatagggtt cggcgcaaag ggtcttgcgg
       61 atgacgccat tgacgtttgc aaccgccttc agcacgccct tgccgagata gcgcgacgga
      121 tccccatcgc gaagctctac cgcctcgcgt gtaccggtgg acgcgccgga aggcaccccg
      181 gcccgtccat ggctgccgcc catcagccag acttccgcct cgacggttgg attaccccgc
      241 gagtcgagga tctcgcggcc ccggatgtcc tttatcctga tcatcgatac cccgggttgt
      301 taaggccgtc gtgcaggcgc gccatgcgcg cgagtgccat ttagctttgc gaactcgccc
      361 gtgcggcggg atcccggacg tttctgttca agccacaatg cgttcacccg agtccgtact
      421 cagattcggc gaagccaagt ctcttcacgc tggcatcaat tgcctgcaac acttcgagga
      481 gagctttcat cttgccgagc ggccaggcat tgggcccatc gctcaaagca ctgtccgggt
      541 ccgggtgggt ctccatgaaa agccccgaaa tgccggcggc cgtcgcagcc cgcgccagca
      601 ccggaatgaa ttcgcgttgg ccgccggatg ccgtccccct tcccccgggc agctggaccg
      661 aatgcgtcgc atcgaacacc accggacagc cggtgccgcg cattatcgcg aggcttcgca
      721 tgtcggacac cagattgttg tagccgaagg aaaagccgcg ttcgcacacc ataatctggc
      781 tgttgcctgt cgagcatgcc ttgtcgacca cattctgcat ctcccacgga gaaagaaact
      841 ggcctttctt gatattgacg ggcttgccct gctccgacac tttcagtatg aaattggtct
      901 ggcggcagag gaaagccggc gtctgcagca cgtcaacgac agcggcgacc tccgccagag
      961 gcgtatcttc gtggacgtcg gtcagcacag gcactcccac ctggcgccgg acctcggaaa
     1021 gaatccgcag gccctcctcg atgcccggac cgcggaagcc tgagcgggag ctgcggttcg
     1081 ccttgtcata cgacgacttg tagatgaatg gcaggttgag cgcagcggtc atctccccga
     1141 gtgcgcccgc cgtgtggacc gtcatggcct cgctttccac gacgcaggtg ccggctatca
     1201 ggaaaaaggg tttgtccggc ccgacctcga acccgcacag gttcatgctt cggcggctct
     1261 tgcttcaacg gtgcgcggca aatctccgct gtggcgcttc ctcgctgcga ggatgaaccc
     1321 ggtgaagaga gggtgcccgt cgcgcggatt ggaggtgaac tccgggtgga actggctgcc
     1381 gatgaaccag ggatgatcgg gcaactcgat catctcgacg agatcgtcca ctgacagtcc
     1441 ggagaaccgc atgcctgctt tctccagccg ttcacggtag ttgttgttca cctcgtagcg
     1501 gtgccggtgc cgttcccata tctgttcctt gccatagatt gccgccgcca gcgagccttt
     1561 ggcgaggtca actcgctggg caccgagccg catcgtaccg cccaagtccg agtcctcgtc
     1621 gcgttcctcc aaggagccgg cactcgtctg ccattcggtg atcagcgcaa tgaccgggtg
     1681 cggcgtcgat ttgtcgaact cggtgctctg cgcaccgctc aacgacgcta cgttccgcgc
     1741 gaattcgatg accgcaacct gcatgccgag acagattccc aggtaaggga cgccgttttc
     1801 gcgcgcgaag cgcaccgcct ggattttgcc ctcgatgccg cggtcgccga agcctccagg
     1861 cacgacgatg ccatccatcc cctccaggca gccgacgccc tttacctcga tgtcctgtga
     1921 ttcgacgtag tggatgccga cgcgggtgcg cgtatgaatg ccgccgtgca gcagcgcttc
     1981 gctgagtgaa atataggagt cacgcaggtc aacatacttg ccgaccatcg cgatgttgac
     2041 ctcgccatcc ggattctgct tggcggcgac gacggcatcc cactcactca ggtcggccgg
     2101 cggaagatcg aggcgcagct gcgacgctac gatctcatcc aggccctctt tctgcatcat
     2161 cgacgggatc ttgtagaggt cgtccacatc ggtcgccgat atgaccgcct tctcgggaac
     2221 attcgtgaac agcgcaatct tgcggcgctg ttcctgcggc aattgctgtt cggaccggca
     2281 taccaggatg tccggctgta tgccgatgga gcgaagctcc tttacggaat gctgcgtcgg
     2341 cttggtcttg atctcggaag acgtcctgat gtagggcacg agtgtcaggt gtacgtaaac
     2401 cacgcgatcg tgtcccagct ccacgcccat ctggcggata gcctcgagga acggcaggga
     2461 ttcgatatcg cctaccgtac cgccaatctc cacgagacag acgtcggcat taccggcgcc
     2521 ccggcggatg ctctctttta tctcatccgt aatgtgtgga attacctgta cggtggcccc
     2581 caggtagtcg ccccggcgct ccttggcgat tacggtttcg taaatgcgcc cggtagtgaa
     2641 attgctgttg cgacccgtgg tcgtgcggat gaaacgctcg tagtggccga ggtccaggtc
     2701 cgtctcgctg ccgtcctccg taacgaacac ctcgccgtgc tggaacgggc tcatcgtgcc
     2761 cgggtccacg ttgatgtacg ggtccagctt gatcatgctg atggagagcc cgcgggactc
     2821 gaggatcgcg ccgagacagg ccgaagtgat gcccttgccc aacgaggaca ctacgccgcc
     2881 agtgatgaat acgtacgatg tcatgcagtg ccctgtgcag acggcggcgg gaaacaacaa
     2941 agggtcagac aaaagtcacg atagagccct tgcgggcatc gtgcctttct tccaaccctt
     3001 tccatacttt cgccgcttgc cgaagctggt caccgggatg agttgcctgc cgctgcgcga
     3061 aagattttgt atcccgtcgc atgaatgaaa gacggtgtta caagttatca gatcgctatc
     3121 gggcagacaa tcgccagcgc gccggcaatc ctatacgtcg cgatattaca gctaaaagag
     3181 tgacggtttg ccttcccagc acacccggaa cccgtcatcg cgcgcgcaat ccgcggcaat
     3241 ccacaggtcc gcgacggcgg caagcgtttc accggcatac agcaacggca cgtact
//
LOCUS       DASUOM010000302         6967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_993706,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000302 DASUOM010000000
VERSION     DASUOM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993706"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFY27_06390"
     CDS             complement(<1..1059)
                     /locus_tag="VFY27_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5765177.1"
                     /translation="MLTGAAELPLPDPESLAHSKRVAAFIRQAIRQAGGSISFAEFMQ
                     HALYAPGLGYYSAGAAKFGASGDFITAPETSPLFAKILARQCAPILQALPCRSVLEIG
                     AGRGALAADLLEYLPDLGSYKILEVSADLRRRQEDLLRQEAPGQVAKVEWLDDLPQNF
                     CGVVIANEVADALPVERFESTGAGLLQHRVVAEGDAFAWRRDAAPPFMEQAVRDLEHS
                     LGTRFPDGYVSELSPGLPGWIADISASLDRGFMFLFDYGLSRAEYYASDRSGGWLRCH
                     FRHRAHSDPLIYPGIQDLSAWVDFTTVAEAATGAGMEVAGFVSQAHFLMNGGLQEELA
                     EFQRLPTAAQLEISRQVKM"
     gene            1198..1953
                     /locus_tag="VFY27_06395"
     CDS             1198..1953
                     /locus_tag="VFY27_06395"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX5765178.1"
                     /translation="MSHEAVTLDRRVALVSGGARRIGAAIARRLHEAGATVAIHYRGS
                     ADEAHRLRDLMNTRRHDSARIFEADLAETQRLPALIESVVDWAGTLDILVNNASSFYP
                     TPLGSVTESQWDDLVGTNLKAPMFLSQAAWPYLRRQGGSIVNLIDVHARRPLRDHAVY
                     GSAKAGLEMLTRSLAKDMAPDVRVNGVAPGAILWPERGMNEVVKRKIIEQIPLKRSGL
                     PDDIAGCVLYLVRDAAYVTGQVIAVDGGRSIGW"
     gene            complement(1954..2475)
                     /gene="folK"
                     /locus_tag="VFY27_06400"
     CDS             complement(1954..2475)
                     /gene="folK"
                     /locus_tag="VFY27_06400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5765179.1"
                     /translation="MARVYLGIGSNVGPVGNLRLGVRELQRQFGAIRLSPVYRSTPLG
                     FEGDDFLNVVVALETDLSPQEVLQVIERIHVRAGRQRDAHKLVSRTLDIDLLLYDDLI
                     LDTPGLKLPRRDVLSYSFVLRPLAELAPEALHPVTGRSFREHWRQLQAGRSEDGVGEH
                     PLEEADVNLLEDE"
     gene            complement(2484..2846)
                     /gene="folB"
                     /locus_tag="VFY27_06405"
     CDS             complement(2484..2846)
                     /gene="folB"
                     /locus_tag="VFY27_06405"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5765180.1"
                     /translation="MTDTIFVRDLRVETVIGIWDWERKIRQTVSIDLEMVADIRRAAA
                     TDSIDDTLNYKAIAKRVQQFVSDSNFQLVETLAEKIAALILEEFPVPSVQVRVNKPGA
                     IRGARDVGVLIRRNKAGE"
     gene            complement(2863..3876)
                     /gene="tsaD"
                     /locus_tag="VFY27_06410"
     CDS             complement(2863..3876)
                     /gene="tsaD"
                     /locus_tag="VFY27_06410"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5765181.1"
                     /translation="MQILGIETSCDETGVAVFEPGRGLLAHALHSQVDLHARYGGVVP
                     EIASRDHIRRLLPLVADVLRQAGVERPDAIAYTAGPGLIGALMVGSGLANGLALAWDR
                     PVLPVHHMEAHLLAPMLEAEAPSCPFLALLVSGGHTMLVAAEGVGHYRLLGTTLDDAV
                     GEAFDKTAKLLGLGYPGGAALARLALKGNERAIDFPRPMLHQGGFDFSFSGLKTAVML
                     QVRAAERDGGLDGLRADIAASFQRAVVDTLVGKTLRAARSERSSSIVVAGGVGANVML
                     RAELARGFEGKVFYPRPEFCTDNGAMIAVAGAFRMADAAPPGQIEARARWPLHSLKPP
                     AAR"
     gene            4043..4258
                     /gene="rpsU"
                     /locus_tag="VFY27_06415"
     CDS             4043..4258
                     /gene="rpsU"
                     /locus_tag="VFY27_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5765182.1"
                     /translation="MPSVRIKENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHLKRVSREVSRRKRLY"
     gene            4398..4841
                     /locus_tag="VFY27_06420"
     CDS             4398..4841
                     /locus_tag="VFY27_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5765183.1"
                     /translation="MLKTRIQEDMKQALKAGDKDRLKVVRMLLAAIKQMEVDRRTELN
                     DSGVLGVLEKMVKQRRESVSQFQAGNREDLVDIELAEIAVLETYLPAPLSEVEVDEMI
                     ATAIADTGATGIRDMGKVMAHLKSRIQGRADMGAVSAKVKAQLSG"
     gene            5009..6793
                     /gene="dnaG"
                     /locus_tag="VFY27_06425"
     CDS             5009..6793
                     /gene="dnaG"
                     /locus_tag="VFY27_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5765184.1"
                     /translation="MPGLIPQHFIDDLIARADIVEVLSTRIQLKKAGREYKAVCPFHD
                     EKTPSFTVSPAKGFYHCFGCGAHGTALGFLMQHDHLEFPDAVESLARMMGIQVPREEG
                     ERPARRYDELFECMAEAERYFQEQLKSHDTAVAYLKQRGIDGPTTKRFAIGYAPPGWS
                     QLLDRMGTSQERIERLLAAGLIIRKDNGSHYDRFRDRLMFPIHDSRGRCIGFGGRVLG
                     DDTPKYLNSPETVLFHKGRELYGLFEARQVLRDIPRMVVVEGYMDVVGLARHGIDFAA
                     GTLGTATTIEHLNRLFRLTDEVLFCFDGDRAGRDAAWRALEVALPEIREGRQMRFVFL
                     PEGQDPDTYIAAHGAQKFEQVLDEAVPLSEFLLNELSAQVDMGSVDGRARLAELARPL
                     IARVPKGIYRELLTSQVAEAVGISAAKLDAIVSRDNSKAKAGHRVTGLSVARQRGIHS
                     ATGSRPSAVRRAITLVLHYPEAAAGVDPAILFGIDRPGAGLLRELIETARSGPNMSMA
                     GLLERFRNHEQARALGKLAAVEMPPAEEFDPATELSDCLAQLITAGARDRAQILIEKE
                     SVSALSDEERSELRRLARRPAAKLGTEG"
BASE COUNT         1393 a         2164 c         2137 g         1273 t
ORIGIN      
        1 catttttacc tgccgcgaaa tttcgagctg tgccgcagtc ggcagtctct ggaactccgc
       61 gagctcctcc tgcagaccgc cgttcatcag gaagtgcgcc tgcgatacaa agcctgcgac
      121 ctccattcct gcgccagtgg cagcttcagc gacagtggtg aaatccaccc acgcgctaag
      181 gtcctggata ccggggtaga tgagcgggtc gctgtgcgcg cgatgacgaa aatgacagcg
      241 caaccagcca ccgctgcggt cactcgcgta gtactcggca cgcgacaaac cataatcgaa
      301 caggaacatg aagccgcgat ccaggcttgc ggaaatatcc gcgatccagc cggggagtcc
      361 cggcgacagc tctgaaacat acccgtcagg aaatcgtgtg ccgaggctgt gctcgagatc
      421 tcttactgcc tgctccataa atggaggtgc ggcatctcgg cgccaggcaa atgcatctcc
      481 ctccgcaaca acacgatgct gcagtagccc ggcgcccgtg ctctcgaaac gttcgacggg
      541 caatgcatcc gcaacttcgt ttgcgataac gacaccgcag aagttctgtg gaagatcatc
      601 cagccattca accttcgcga cctgcccggg cgcttcctgg cgcagcaggt cttcctggcg
      661 tcggcgcaag tcggccgaaa cctccaggat cttgtagctg cccaggtccg gaaggtattc
      721 aaggagatcc gccgcaagtg caccgcgccc tgctcctatt tccagaacgc tccggcaggg
      781 cagcgcctgc aaaatcggcg cgcattgccg cgccaggatc ttcgcaaaca ggggcgaagt
      841 ctccggcgcg gtaatgaagt cgccgctggc gccgaatttt gctgcacccg cactgtagta
      901 accgagcccc ggagcgtaca aggcatgctg catgaactct gcaaaactga tgctgccacc
      961 ggcttgccgt atcgcctgcc tgatgaacgc cgcgactcgc ttgctgtgag caaggctctc
     1021 gggatcgggc aaaggcaatt ctgctgcgcc tgtcagcata gaggcatcgt gtacttggtt
     1081 tcgcaaaatc ggcacaatgt attgccccat catgctcaca tgcaaacttt tacggccagc
     1141 agtcggtacc aaccttgtca gcaaatctcc tgaatcaccc atgatcgaca gcgaaaattg
     1201 tcacacgagg ccgtaaccct tgaccgcagg gtcgctctcg tttcaggcgg cgcgcgcagg
     1261 ataggcgccg ctatcgcgag aaggctgcac gaagccggcg ccaccgtcgc cattcactat
     1321 cgaggatccg cggacgaggc gcatcgcttg cgcgatctga tgaacaccag gcggcacgac
     1381 agcgcgagga tcttcgaggc tgacctggct gagacgcagc gcctgccggc gctcatcgaa
     1441 tcggtcgtag actgggccgg tacgctggac atcctggtca acaatgcatc ttccttctat
     1501 cccacgccgc tgggttccgt tacggagagc caatgggatg atctcgtggg gaccaacctc
     1561 aaggccccga tgttcctctc ccaggcggca tggccctacc tgcgtcggca gggcggaagc
     1621 atcgtgaacc tgatcgacgt gcacgctcga cggccgctgc gcgatcacgc cgtttatggt
     1681 tccgcgaagg ccgggctgga gatgcttacc cgctccctgg cgaaggacat ggcgccggac
     1741 gtgcgggtga acggcgtcgc acccggggcg attctctggc cggaacgggg tatgaacgag
     1801 gtggtgaagc ggaaaatcat cgagcagatt ccactgaaac gcagcggact gcccgacgac
     1861 atcgcggggt gcgtgctcta cctcgtccgc gacgccgcct acgttacagg gcaggttatt
     1921 gccgtcgacg gcggccgcag catcggctgg tgatcattca tcctccaaca ggttgacgtc
     1981 tgcttcttcc agcggatgct cccctacacc gtcttcgctt ctacccgctt gcagctggcg
     2041 ccagtgttcc cggaaactcc gacccgtgac cggatgcagc gcttctggcg cgagctccgc
     2101 gagcgggcgc aggacgaagc tgtagcttag aacatcacgg cgcggcagct tcaggccggg
     2161 cgtgtccagg atgaggtcgt cgtacagcag caggtcgata tccagagttc gcgacaccag
     2221 cttgtgtgcg tctcgctgcc gacccgcgcg gacatgaatc ctctcgatca cctgcagtac
     2281 ttcctgcggc gatagatccg tctcgagggc cactacgaca ttgaggaagt cgtcaccctc
     2341 gaagccgagc ggtgtacttc ggtacaccgg tgacaacctg attgcgccaa actgccgctg
     2401 cagctcgcgg actccgaggc gcaggttccc gacggggccg acattgctgc cgatcccgag
     2461 atagaccctg gccatcggac ggattattcc ccagccttgt tgcggcggat cagcacaccc
     2521 acgtcccgcg caccccggat cgctcccggc ttgttgaccc gaacctgtac cgagggcacc
     2581 gggaactcct cgaggatcag ggccgcgatt ttctcggcca gtgtctctac cagctgaaag
     2641 ttcgagtcac tgacgaactg ctgcacgcgc ttggctatcg ccttgtaatt aagcgtgtca
     2701 tcgatgctgt cggttgccgc tgcacgacgg atgtccgcga ccatctccag gtcgatgctg
     2761 acggtttgcc tgatcttgcg ttcccagtcc cagatcccga tgactgtctc gacccggaga
     2821 tcgcgaacaa atatggtgtc ggtcattcgc ctggttcctt tttcacctgg cggcgggcgg
     2881 cttcagtgag tgaagcggcc agcgagcccg cgcctcgatc tgcccgggcg gcgcagcgtc
     2941 cgccatcctg aaggccccgg caacggcgat catggcaccg ttatccgtac agaactccgg
     3001 gcgaggataa aacactttgc cctcgaatcc tcgtgcgagc tctgcgcgga gcatgacgtt
     3061 ggcgccgacg ccacccgcga cgacgatact gctgctgcgt tcgctgcggg ctgctcgcag
     3121 cgtcttgccg accagcgtat cgacgacggc gcgctggaaa gacgccgcga tatccgcgcg
     3181 aaggccgtca agcccgccgt cacgttccgc agcgcgaacc tgcagcatga ccgcggtctt
     3241 caaaccgctg aagctgaagt caaagccgcc ctggtggagc atggggcgcg gaaagtcgat
     3301 ggcgcgttca tttcccttaa gcgccagccg ggcgagagct gctccgccgg gatagccgag
     3361 gcccaggagc ttcgcggtct tgtcaaaagc ctccccgact gcatcgtcga gcgtcgtgcc
     3421 gaggagccga tagtgcccca cgccttccgc tgcgacgagc atcgtgtgtc cgccggatac
     3481 gagcagggcc aggaagggac aggaaggtgc ctccgcctcc agcatcggcg ccagcaggtg
     3541 cgcctccata tggtgcaccg gcaggaccgg tcgatcccat gccagcgcca ggccgttcgc
     3601 aaggccgcta cccaccatca gcgcgccgat gaggccggga cccgccgtgt aagcgattgc
     3661 atcgggccgc tccacccccg cctgccgaag cacatccgcg accagcggca gaagccgcct
     3721 tatatggtcc ctcgaagcta tttccggcac tacaccgcca taccgagcat gcaagtcgac
     3781 ctggctgtgc aaggcgtgcg cgagcaagcc gcggccgggc tcgaaaacag ccacgccggt
     3841 ctcgtcacag ctcgtttcaa tgccaagaat ttgcatacgc ggcggatcat acttgaagga
     3901 gccggatccg cagagcctgc acccgcttcg cgcgagctgc cggcggagct ttgcaatatg
     3961 tcccgcgggg ggtatcatgt gcggctctct cccggcccca gtgccgggtt tatttattgc
     4021 cgcagctgga accgattgtc atatgccgag tgtccgcatc aaagagaacg aatatttcga
     4081 cgccgccctg cggcgcttca agcgagcctg cgagaaagcc ggcgtgctga cggagcttcg
     4141 ccgtcgcgag ttttatgaaa aaccgaccca ggagcgcaaa cgcaagaagg cggctgccgt
     4201 aaagcgccac ctgaagcgag tgtcccgcga agtctccagg cgcaagcgac tgtactgatc
     4261 ttcctggctg acggtgtcgt gacggccgcc cgattacctc aacccgttga cgcacgattc
     4321 gtcccccgtc gctcgtccgt agatcggcaa ttccgtatgc tgcgccgcag gttattgccg
     4381 cagagaccgg cacattgatg cttaagaccc gaatccagga agacatgaag caggctctga
     4441 aagccggcga caaagaccgg ctgaaagtgg tgcgcatgct gctcgcggcg atcaagcaaa
     4501 tggaggtcga caggcggacc gagctgaatg attccggagt gcttggtgtc ctggagaaga
     4561 tggtcaagca gcggcgggag tcggtcagcc agttccaggc aggcaaccgg gaagacctcg
     4621 tggacatcga gcttgccgaa attgcggtcc tggagacgta cctgccagcg ccgctctcgg
     4681 aagtcgaggt cgacgaaatg atcgccacgg ccattgcgga cacgggcgca accggcattc
     4741 gtgacatggg gaaggtcatg gcgcatctga agtcccgtat ccagggtcgg gcggacatgg
     4801 gtgccgtgag cgccaaggta aaagcgcagc tgtcagggta agagatcgtt cacggcggga
     4861 attgccctca aaaaagtact ggcggaccgc cctgctttgg ctatgcttgg caacagaacc
     4921 ggtcacgcca ggaaaaaaac caggtacacc tcggccgtcg tgggtccctg tatcaagtaa
     4981 tctgcagaac cgcgaattcc ctcgaatcat gcccggactg ataccccagc acttcattga
     5041 cgacctgatc gcacgggcgg atatcgtgga ggtcttgagc acccgcatcc agctgaagaa
     5101 agcgggccgg gagtacaagg cggtctgccc gtttcacgat gaaaaaacgc cgtcctttac
     5161 cgtcagtcca gccaaggggt tctatcactg cttcggctgc ggcgcacacg gcacggcgct
     5221 cggcttcctg atgcagcacg atcacctcga gttcccggac gcggtggaat cgctcgctcg
     5281 catgatgggc atccaggtgc cgcgggaaga aggcgagcgg cccgcgcgcc gctacgacga
     5341 gctgttcgaa tgcatggccg aggccgagcg atatttccag gagcagttaa agagtcacga
     5401 cactgctgtc gcctacctca agcaacgcgg gattgacggg ccgactacca aacgtttcgc
     5461 aatcggctac gctccgccag gctggagcca gttgctggac cggatgggaa cctcgcaaga
     5521 acggatcgag cgcctgcttg cagcgggcct catcatccgc aaggacaatg gcagccacta
     5581 cgaccgtttt cgcgatcgcc tgatgttccc gattcatgat tctcgtggcc gatgcatcgg
     5641 cttcggcgga cgagtcttgg gggatgacac gccgaagtac ctgaactcac cggaaacggt
     5701 cctgttccac aagggccgtg agctttacgg gctgttcgag gcacgccagg tcttgcggga
     5761 tatcccccgg atggtagttg tggaaggtta catggatgtc gtggggctgg cgcgccacgg
     5821 gattgatttc gcggctggga cgctcggcac ggcgacgacc atcgagcacc tgaatcgcct
     5881 gttccggttg acggatgagg tcctcttctg ttttgacgga gatcgtgccg gccgcgacgc
     5941 ggcctggcgc gcgctcgagg tcgcgttgcc cgagattcgc gagggccgcc agatgcgttt
     6001 cgtcttcctg cccgaaggtc aggacccgga cacctacatc gccgcacacg gcgcgcaaaa
     6061 gtttgagcag gtcctggatg aagcggtccc gctgtcggag ttcctgctga atgagctgtc
     6121 ggcacaggtg gacatgggaa gcgtcgatgg cagggcccgg ctggctgaac tcgcgcgtcc
     6181 gctcatcgcg agggtgccta agggcattta cagggaactc ctgacgtccc aggtcgccga
     6241 agcggtagga atttcggcgg cgaagctgga cgcgatcgtg tcccgggaca actcgaaagc
     6301 gaaggccggc caccgggtta cgggcttgag tgtcgccagg cagcgaggga tacattcggc
     6361 aaccggcagc aggccgtcgg ctgtgcggcg agcgattacg ctcgtactgc attatcccga
     6421 agcggcagcg ggcgttgatc cggcgattct cttcggcatc gatcgtccgg gtgccgggct
     6481 cctgcgggaa ctcattgaaa ccgcgcggtc cggtcccaat atgagcatgg ccggcctgct
     6541 ggagcgtttc aggaatcacg agcaggcgcg agccttaggc aaactcgctg cagttgagat
     6601 gccgcccgcc gaagaattcg accccgccac cgaactgtcg gactgcctgg cgcagctcat
     6661 caccgccggg gcacgagatc gggcacaaat tctgattgaa aaagaatcag ttagcgccct
     6721 gtctgacgaa gagcgcagcg agttgcgacg cctcgcacgg cgcccggcgg caaagctggg
     6781 aactgaggga taattctgga ttggactctg tcaaaagagt acacttcgtc gtttgcattt
     6841 tttacttgga ggttcaagga cttggccgaa aaacgcgcaa ttattagcgg caacaaacag
     6901 acgcagcagc taaaagatct gattgcccgc ggcaaggagc agggttacct gacctatgct
     6961 gaagtca
//
LOCUS       DASUOM010000303         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1368709,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000303 DASUOM010000000
VERSION     DASUOM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368709"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFY27_06430"
     CDS             <1..426
                     /locus_tag="VFY27_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765185.1"
                     /translation="ETQAKDIRLGQSVDVDTRNGVVEGRVTRIDPAVVNGTVQVDVEI
                     LGELPPGARPDLSVDGTILIERLDDVLHVGRPAYGQPGSTLRLFRLQPQSGVAVKVPV
                     SLGKVSVNRVQILDGLTEGERVVLSDTSQWDDHDRIELN"
     gene            484..1233
                     /locus_tag="VFY27_06435"
     CDS             484..1233
                     /locus_tag="VFY27_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765186.1"
                     /translation="MAETQDALIRLTDVKKIFYTDEVETHALAGVHAEIMRGEYVAIM
                     GPSGCGKSTLLSILGLLDTPSAGRYMLNGTSVEDINASTRARIRNKEIGFIFQAFNLI
                     GDLTVFENVELPLTYRDGMPRAERKERVMASLERVGMSHRVKHYPAQLSGGQQQRVAV
                     ARALVGQPSILLADEPTGNLDSKNGDAVMKLLDELHGDGATICMVTHDPRFAECAKRR
                     IAMFDGQVVDEETLEELRASEDLRASVNERA"
     gene            1354..3822
                     /locus_tag="VFY27_06440"
     CDS             1354..3822
                     /locus_tag="VFY27_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765187.1"
                     /translation="MHAVLDDLRFASRALRARPGFVIAAVLTLALGIGANTAIFSVIN
                     AFMLKPLPYPDGERLVEVHNTYPGDGLENAGVSIPDFIDRRDQAVALADSALFTWESF
                     NLAMSGTPERLTGLVATPSLFSTLQVSPVIGRPFSAEEAIPGNAKVVVLTYDLWQNRF
                     NGDRNIAGSDIRLNGEQYRVVGVMPRGFSFPNREIQLYVPFAFTQEQMSDESRGSEFS
                     MSIGRLAPGATIEQLNSQIDSIVQSNKDRFIGISERGAAYSDFLERSGFTGRALSWRD
                     YLVGDVQTTLLVLQGVVVFVLLIACANVANLMLSRVLGRARELAMRSAVGAERTRVAR
                     QLVLEGVLLGLAGGVLGLILSFVIIQLIEVFGIDRSSQNFDVVIDPYVLLFAFLVSVA
                     AGVAFSLISVFAAWRMDTQRVIREGGGQTASRGSLTARGVLVSFQVALAVTLLVGAGL
                     LLRSFQNLQEESPGFEPEGVVALQLELTGQQYADDDQRRVFLDSMLENIRQQPGITEA
                     GITTVLPFNRDGSQGSYSIEGYTRASGESSPHGHQRIVSEGYFRAMNIPLLQGRSFDP
                     SLDGADAPSVVIIDELLARKYFRDTSPLGRRISNLEDEEGNDIWSTIIGVVGTIKHDR
                     LNEETSKETYFFYSRQEVPGRASVVVRTSLPPATAGRQIRDAVLAADPEQPVFGMMTM
                     DERIARSLGEQRAPTMLLSMFSVVAVLLAVVGIYGVLSYSVGQRTTELGVRMALGAET
                     GNVLKLVLGQGAWLIGAGLLAGVLAALALTRFLGSLLFGISMFDPLTYGLVALLLVAV
                     GVAACGLPAWRATRISPVAALRYQ"
BASE COUNT          714 a         1171 c         1209 g          737 t
ORIGIN      
        1 gaaacgcagg ccaaggacat tcgccttgga caatccgtcg acgtggatac gaggaacggc
       61 gtcgtcgaag gccgcgttac ccgcatcgat ccggccgtag tcaacggtac cgtgcaggtc
      121 gatgtcgaga tcctgggcga actgccgccg ggcgcgcgcc cggatctgtc ggtagacggg
      181 accatcctca tcgagcggct cgacgacgta ctacacgtcg gccgtccggc ttacgggcaa
      241 cccggttcga cgctgcgcct gtttcgcttg cagcctcagt ccggggtcgc cgtgaaggtg
      301 ccggtgtcac tcggcaaggt ttccgtaaac cgcgtgcaaa ttcttgacgg actgaccgaa
      361 ggcgagcggg tggttctttc ggacacatcg cagtgggacg accacgaccg catcgaactc
      421 aactagtaca ccaactgaac gcatcaatat catcttgaca gagtattaag gggagccagg
      481 accatggccg aaacgcaaga cgcgctgatc agactgacgg acgtcaagaa aatcttttac
      541 acagacgaag tggagacgca cgcgctggct ggtgtgcacg ctgaaataat gcgcggcgag
      601 tacgtcgcga tcatgggtcc ttccggatgc ggcaaatcga cgctgctcag catcctcggt
      661 ttgctggata cccccagcgc cggacgctac atgctcaacg gcacctccgt cgaagacatc
      721 aatgcgagca cgcgcgcccg catccgcaac aaggaaatcg gtttcatctt ccaggcattc
      781 aacctgatag gagacctgac ggtcttcgag aacgtcgaac tgccgctcac ataccgcgac
      841 ggcatgccgc gcgccgagcg caaggaacgc gtaatggcct cgctcgaacg cgtcgggatg
      901 tcgcatcgcg tcaagcacta ccccgcgcag ctgtcgggcg gccagcagca gcgcgttgcc
      961 gtggcccggg cactggtagg tcagccctcg atcttgctgg ccgacgagcc gacgggaaac
     1021 ctcgattcca agaatggcga tgcggtcatg aagctgctcg acgagctgca tggggacggc
     1081 gccacgatct gcatggtgac ccacgacccc cgatttgcgg aatgcgcaaa acggcgtatc
     1141 gcaatgttcg acgggcaggt ggtggacgaa gagacgctgg aggaactgcg ggcgagcgag
     1201 gacctgcgcg ccagcgtgaa cgaaagagct tgagcaggtg gaattagaag cgacagtagg
     1261 agcccgcaac gcgggcgagt tcgccgagcg tcatcgcact cgcccgcatg gcgggcttct
     1321 acgacctttt gcagggtgct cagggagaaa agcatgcatg cggtactaga cgatcttcgg
     1381 tttgcctctc gcgcactgcg tgcgcggccg ggcttcgtga tcgcggccgt gttgacgctg
     1441 gcgctcggaa tcggcgcgaa cacggctatt ttcagcgtca tcaacgcgtt catgctgaag
     1501 ccgctgccgt accccgacgg cgaacggctg gtcgaagtgc acaacaccta tcccggcgac
     1561 ggcctcgaga acgccggcgt ttccatcccg gacttcatcg atcggcgcga ccaggcggtg
     1621 gcactcgccg attcggcgct cttcacctgg gagtcgttca acctcgcgat gagtggcaca
     1681 cccgagcgcc tgaccgggct ggtggccacg ccctcgttgt tctccacctt gcaggtgtca
     1741 cccgtgatcg gccgtccctt cagcgccgaa gaggcgattc cgggtaatgc gaaggtcgtt
     1801 gttctcactt atgacctgtg gcaaaaccgc ttcaacggcg accgcaacat cgcaggcagc
     1861 gatatccggc tgaacggcga gcagtaccgc gtggtcggcg tcatgcctcg agggttttcg
     1921 tttccgaatc gtgaaataca gctctacgtg ccgttcgcat tcacacagga gcagatgtcc
     1981 gacgaatcgc gcggcagcga attttcaatg agcatcggcc ggttggcccc cggggccacg
     2041 atcgagcagc tgaattccca aatcgattcg attgtgcagt caaacaagga ccgcttcatc
     2101 ggcatatccg agcgcggtgc ggcctactcg gatttcctcg agcgcagcgg ctttaccggg
     2161 cgcgccctgt cctggcgcga ctacctggtc ggtgatgtgc agacaacctt gctggtcctg
     2221 caaggagtgg tcgtcttcgt gctgctgatc gcctgcgcga atgtggcgaa tctcatgctt
     2281 tcacgggttc tgggccgcgc acgcgaactc gcaatgcgct ccgcggtcgg tgcagaacgc
     2341 acacgggtcg cacgccagct cgtgctcgaa ggcgtgctgc tggggttggc cggcggcgtg
     2401 ctggggctga tcctgtcgtt cgtcatcatc cagctcatcg aggtattcgg catcgaccgc
     2461 agctcgcaga atttcgatgt ggtcatcgat ccttacgtgc tgctatttgc ctttcttgtt
     2521 tccgttgctg ccggcgtcgc gttcagcctc atctcggttt tcgccgcctg gcgcatggac
     2581 actcaacgtg taataaggga aggtggcggg cagaccgcaa gccgcggctc gcttacggcg
     2641 cgcggtgtgc tggtgtcatt ccaggtagca ctcgccgtaa ctctgctggt cggcgccggg
     2701 ttgttgttgc gcagcttcca gaatctgcag gaagagtcgc ccggattcga accggaaggc
     2761 gtggtagcac tgcaactcga acttaccggg cagcaatacg cagacgacga ccagcggcgc
     2821 gtattcctcg acagcatgct cgaaaacatc aggcagcagc cgggaatcac ggaagcgggg
     2881 ataactaccg tgttgccatt caatcgcgat ggctcgcagg gcagctatag cattgaaggt
     2941 tacacccgtg cctcgggtga aagctcgccg cacggacacc agcgcatcgt cagcgaaggc
     3001 tacttccgcg ccatgaatat tcccctgttg cagggccgtt cgttcgatcc atcacttgat
     3061 ggcgcggacg ctccgagcgt cgtcatcatc gacgagttgc tcgccaggaa gtatttccgc
     3121 gatacgagtc cgctcggccg gcggatcagc aacctggaag acgaagaagg aaacgatatc
     3181 tggtcgacca tcatcggtgt ggtcgggacg atcaagcacg accgtctgaa cgaagaaacg
     3241 agcaaggaga cctacttctt ctactcgagg caggaagtgc ccggccgcgc atccgtcgtc
     3301 gtgcgcacgt ccttgccgcc tgccacggcc ggccggcaga tccgcgatgc cgttttggct
     3361 gccgatccgg agcagccggt gttcggcatg atgacgatgg acgaacgcat cgcacgctcg
     3421 ctcggcgaac agcgcgcgcc gactatgctg ttgtcgatgt tctcggttgt tgcggtgctg
     3481 cttgcggtcg tcggcatcta cggagtcctc tcgtattccg tgggccagcg caccacggag
     3541 ctcggcgtgc gcatggcgct cggcgccgaa accggcaacg tcctcaagct ggtgttgggg
     3601 caaggcgcct ggctgatcgg cgcgggcctg ctcgccggcg tactggccgc gctggcgctg
     3661 acccgtttcc tcgggtccct cctgttcggg atttcgatgt tcgacccgtt gacttatggg
     3721 ctggtggcgc tgcttctggt tgccgtcggc gttgccgcct gcgggctccc ggcgtggcgc
     3781 gcgacgcgca tcagcccggt agcggccttg cgctaccaat gagcgggtaa a
//
LOCUS       DASUOM010000304         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_155279,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000304 DASUOM010000000
VERSION     DASUOM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155279"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFY27_06445"
     CDS             complement(<1..757)
                     /locus_tag="VFY27_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765188.1"
                     /translation="MPKETGVSKAVRVWLSVGTSVLVGAAGAGCSDGSGEAARNPGPA
                     SGVAEQAAAGPAPAATLPGPAAGGEGEGEGASGADPVDDDVEYLHQLGQTRGHLAAFT
                     ALHRLGEHDMSMTHAKHPESELYTGLKPAFEARGKAGFAEELDALTDAVANGGDVDAA
                     YEDLVKVIARNEPDVDTATHLMAIATLARTAGKEFAIGVADDGRVVNAHEYQDAYGFL
                     TAAKEMLANQEPHNEQEEQALATAAQQLDIAVGE"
     gene            834..1493
                     /locus_tag="VFY27_06450"
     CDS             834..1493
                     /locus_tag="VFY27_06450"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX5765189.1"
                     /translation="MIFRIASVLDELEVRALKDFAAGQVFERGARTAGWSARTVKDNE
                     QLTRGPAATAVSRTVEAALRRNDVFVAAAQPKTFCRTMLSRYEQGMSYGAHIDDALIA
                     GIRADLSFTLFLSPPDSYEGGELVLDTPDGETSLKLDEGDLVLYPTTFLHRVEEVQSG
                     TRLVCVGWIRSLIRLQAHREILFDLETAARTVFASEGKSPLFDRLSRSKSNLLRLWAE
                     D"
     gene            1542..2057
                     /locus_tag="VFY27_06455"
     CDS             1542..2057
                     /locus_tag="VFY27_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765190.1"
                     /translation="MKKKQEDKEQEPQPRVDPKALIAAQSVKSAVLAAIVTMLVLSGF
                     WTFFTGMTGRVFPWFSIVQGIFIGLAVRRFGRGLDWRFPLIAGTVAWSGAFFGNLMLA
                     IPVTTSELGASALQVIRGLTWWSFRTFFTEVITVVDHIYAFCAAAVAVFYSNRRLTRH
                     EDFALRTRQAR"
     gene            2054..2674
                     /locus_tag="VFY27_06460"
     CDS             2054..2674
                     /locus_tag="VFY27_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5765191.1"
                     /translation="MNEKIKIYGDPISGNCYKIKLLCSELGIDYDWHELDILSGATRT
                     ADFLKMNPNGKIPLLELPDGRHLAESNAILCYLAEGTVLAGADRFERATVLQWMFFEQ
                     YSHEPYVATSRFIVRYLGNPPERWDDLEQKRAAGERALGIMELKLRQSPWMTGDRFTL
                     ADIALFAYTHVAEEGGFHLERFPGVMDWIARTMSRPNYKPMYERVE"
     gene            2769..3014
                     /locus_tag="VFY27_06465"
     CDS             2769..3014
                     /locus_tag="VFY27_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5765192.1"
                     /translation="MNIYVGNLAYGTTDDDLRAAFAPYGAITSARVVFDKETGRSRGF
                     GFVEMASQSEALKAIEALNQTELQGRQLRINEARPKR"
     gene            complement(3053..>3489)
                     /locus_tag="VFY27_06470"
     CDS             complement(3053..>3489)
                     /locus_tag="VFY27_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765193.1"
                     /translation="ADRCYLEKCRDRLYNEFVVGGVAVERVQAGEYAVRYESGIDLLR
                     KTPVFYQQVSRDRLYSKFNRAYRYIEVLYDGENPYVDAIRNNMAHLIRILKTGDWDML
                     RRTPPYFVGVDDAERDLAQLAEMQLAEVPKARRPQADNIAAA"
BASE COUNT          674 a         1098 c         1009 g          708 t
ORIGIN      
        1 actcgccgac ggcgatgtcc agctgctgcg ctgccgtggc caatgcctgc tcctcctgct
       61 cattgtgcgg ctcctggttc gccagcattt cttttgcggc agtcaggaat ccataggcat
      121 cctggtattc gtgggcattc acaacccggc catcatccgc caccccgatc gcaaactcct
      181 tcccggcggt gcgtgccagg gtggcgatcg ccatcaagtg cgtcgctgtg tccacatccg
      241 gctcgttcct ggcgataacc ttgacgaggt cctcgtaggc ggcatccaca tccccgccat
      301 tcgcgaccgc atccgtgaga gcgtccagtt cctctgcgaa acccgccttg ccgcgagctt
      361 cgaaggccgg cttgaggcca gtgtagagtt cgctctccgg gtgcttcgcg tgggtcatcg
      421 acatgtcgtg ctcacccagg cgatgcagcg ccgtgaacgc agcaaggtgt ccccgggtct
      481 gacccagctg gtgaagatac tcgacgtcgt cgtctaccgg gtcggctccc gatgcgccct
      541 caccctcacc ttcaccgccc gccgcgggtc caggcaacgt ggccgccggg gcgggtccgg
      601 cagcggcctg ttcggcaacg ccgctcgctg ggccgggatt cctggcggcc tcgccggacc
      661 cgtcagaaca tcctgctccc gctgcaccga ccaataccga ggtaccaacg ctcagccaga
      721 ccctgacggc tttggacaca cctgtctcct tcggcattcg tttctcctat gcgatatgct
      781 ggaaatcaag acttaacgca aatgacaatc atttgcaaca accgtgagca gtgatgattt
      841 ttcgtatcgc gagcgtgctc gacgaactcg aggtcagggc cctcaaggat tttgccgccg
      901 gccaggtttt cgaacgcggt gcaaggacgg cgggttggtc ggcccgcacg gtgaaggaca
      961 atgagcagct gacgcgcggc ccggcggcga ccgccgtcag ccgcaccgtg gaagccgcgc
     1021 tgagacgcaa cgacgtgttt gtcgccgcgg cccaaccgaa aaccttttgc cggacgatgc
     1081 tgagtcgcta cgaacagggc atgtcgtacg gagctcacat cgacgatgcg ctgatcgcag
     1141 gaatacgcgc ggatctttct ttcaccctgt tcctctcgcc gcccgattct tacgaaggtg
     1201 gcgagctggt cctcgacacc ccggacggtg aaacatcctt gaagctcgac gaaggtgacc
     1261 tggttctcta cccgaccacc ttcctgcatc gggtcgagga agtgcagagc ggcacccgcc
     1321 tggtctgcgt cgggtggatt cgaagcctca ttcggttaca ggctcatcgt gaaatcctgt
     1381 tcgacctgga gacggctgcg cgaacggtgt ttgcgagcga gggcaaatcg cccctcttcg
     1441 atcgtttatc gagatccaaa tccaacttgc tcaggctctg ggcagaagac tgagtaaact
     1501 tcacgcgttg ccgccgatcg cacgaggacc ggatgttcgg gttgaagaaa aagcaagagg
     1561 acaaggaaca agaaccgcaa ccgcgggtcg acccgaaggc gcttatcgcc gcccagtccg
     1621 tcaaatcggc cgtcctggcg gccatcgtta ccatgctcgt gttgagcgga ttctggacat
     1681 tcttcacggg tatgacaggt cgggtctttc cctggttctc tatcgtccag ggaatcttta
     1741 ttggcctcgc cgtccgccgc ttcggccgcg gactggactg gcgatttccg ttgatcgccg
     1801 ggacggtcgc atggtccggt gcgttcttcg gcaatttgat gctggcgatt cctgtgacga
     1861 cgagtgaact cggggccagt gcgctgcaag tcatccgtgg gctgacctgg tggtctttcc
     1921 gcaccttctt caccgaagta atcacggtcg tcgatcacat ttacgccttt tgtgccgccg
     1981 cagtcgcagt cttctactcg aacaggcgac tgacccgaca cgaggacttt gctttgcgga
     2041 cgaggcaggc gcgatgaatg aaaagataaa aatctatgga gatcccattt ccggcaactg
     2101 ttacaagatc aagcttcttt gctcggaact cggcatcgac tacgactggc atgaactaga
     2161 catcctttcg ggcgccacgc gaactgctga tttcctcaaa atgaacccga acggcaagat
     2221 tcccctgctg gagcttccgg atggccgcca ccttgcggag tcgaatgcga tcctgtgcta
     2281 cctggccgaa ggaaccgtgc ttgcgggagc cgatcgtttc gagcgagcca ccgttctgca
     2341 atggatgttt ttcgagcaat acagtcacga gccctacgtc gcgacctccc gcttcatcgt
     2401 gcgctacctc ggcaatcctc ccgaacgatg ggacgacctc gaacagaaac gggctgcggg
     2461 agagcgggcg ctcggcatca tggaactcaa gcttcgacaa tctccgtgga tgacgggtga
     2521 caggttcacc ttggccgaca tcgcactgtt cgcctatacc cacgtggcgg aagaaggcgg
     2581 cttccacctg gagcgctttc cgggtgtcat ggactggatc gcgagaacca tgtcgcgccc
     2641 gaactacaag ccgatgtacg agcgagtgga atgaccaacg accgttgtgg attcccaacg
     2701 tccccgctac tatgatcgcg ttgtcaacag aacaactcca atcgaccctc tactgaaaga
     2761 aatcgactat gaacatctat gtggggaacc ttgcctacgg cacgaccgac gacgacctgc
     2821 gcgcagcgtt cgccccctac ggggcgatca cgtccgctcg cgtcgtgttc gacaaggaaa
     2881 caggccgctc gcggggattc ggcttcgttg agatggccag ccagagcgaa gcactgaaag
     2941 ctatcgaagc cctgaaccag acggaactgc agggacgaca gctgcgcatc aacgaggcgc
     3001 ggcccaaacg ctaggagcca ggaggacgta aactgcgcgc gtaccggccg ggtcaggcgg
     3061 ccgcgatgtt gtctgcttgc ggccgtcgtg ccttcggcac ttccgccagc tgcatttccg
     3121 ccagttgcgc aaggtcccgc tccgcgtcgt cgacacccac gaaatagggc ggagttctcc
     3181 gcaacatgtc ccagtcaccg gtcttcagta tcctgatcag gtgcgccatg ttgttacgaa
     3241 tcgcgtccac gtacgggttc tccccgtcat acaggacttc gatgtagcgg taggcgcggt
     3301 tgaacttcga atagagccgg tctcgcgaca cctgctggta gaagaccggg gttttcctca
     3361 ggaggtctat ccccgattca tagcgcacgg cgtactctcc tgcctgcacg cgctccacgg
     3421 caacgccgcc aacgacgaac tcattgtaga ggcggtccct gcacttctcc aggtagcagc
     3481 ggtcggcca
//
LOCUS       DASUOM010000305         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1342211,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000305 DASUOM010000000
VERSION     DASUOM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342211"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFY27_06475"
     CDS             <1..430
                     /locus_tag="VFY27_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765194.1"
                     /translation="DYAAIVRQRRRNFSLLAELLAESGIAPWRALEAGVCPLFFPLLV
                     KDKRAASRKLKESGVIATELWNEGDPLSAAHEGSGAKFLRRHLLELPIHQDVDEAQIR
                     YMAAQVCEGEIALESAVNASLRLAGGRRIRSTHQPIASSG"
     gene            441..1214
                     /locus_tag="VFY27_06480"
     CDS             441..1214
                     /locus_tag="VFY27_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5765195.1"
                     /translation="MSQEATSLESRHEVERRFHDERMAGRQADDRDFYAVGGMDLVLN
                     AYHEAMGDVCGKTVLDFGCGDGARSLEYAKRGATVYAFDISPESVRNLVVTAQSAGLA
                     DRIHASVMPAETLAYPDGMFDLVLGVSILHHTDVSMVEREIERVLKPGGRALFIEPLG
                     HNPFLRLFRLLTPNRRTPTEQPMTVGQIRRLIGRFRQGRFRGFHFLSIVPPGLLWATG
                     NRALFQTMMRMTQTADRWLLAAAPPLRRYCWMTLIDVSK"
     gene            1370..2515
                     /locus_tag="VFY27_06485"
     CDS             1370..2515
                     /locus_tag="VFY27_06485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765196.1"
                     /translation="MSLRVDLIEDDDTFLRMGSVWNRLIDEAGIDHPFLRHEWVRTWW
                     ECFKPAGKLHILVVKEEDEPIAIAPLMFDQGRVYGCSVRRLRGIANVYTERFDLILTR
                     RAKEACRAIWQFLSAEASQWDMLELRQVPENARAAEYLRFWAYEDNFLLGQWHSSDGP
                     FVPIDQPWDSYIKTLSKKHLSNLRGRAKGLHRIGDVRHEIVTGGEQLGRTLDEAFLLE
                     AAAWKGKAGTAIMNRPDRLAFYRSLLTRAAEGGWLRLHFLTIEGKRIAVQIALEIHNK
                     LYILKSGYDPHYAPFAPSLLLCEWMLRDAWKRKLSEVDFLGDAERWKLEWTSQTRAHS
                     WVFIFPNRMRTRWLHRFKFSLLPRLYEHPLYQVVRSAGRRLGLRLHD"
     gene            2543..>4091
                     /locus_tag="VFY27_06490"
     CDS             2543..>4091
                     /locus_tag="VFY27_06490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765197.1"
                     /translation="MNALAIHRVGHWCYQRRIPLIPQVAYGAIFLLYRAVIPMSVEFG
                     EGTELAYGGLGIVLHERTKIGRYVSIGHDVTIGGRSRRWGVPVIADRCVIGAGAKLLG
                     PISVGEASVIGANAVVLDDVPPNSVMVGLPAQVVRQGIDIHDYSCLQPPEEADRLSFR
                     TSNNLLITIIEDAGRLSHLVDEWTDLLEHSEQDCLFLTPEWLTTWWRCFERDEWTLRL
                     VTVRRQSQLLGIAPFFSRQRRFGGFLSHQSTEFLGTGVVGSDYLDLIVRRGEEDMVRV
                     GLTDYFNRSKPLMTLSHLPARAAEADGMVKELELSGWQVRRSTIESCPYITLRGHTWE
                     SYLDSLGANHRYNFTRRLKNLRKAGVLEFDVVEKEDQRPEALQAFLSLHAVAWRERGG
                     SQAMQTARELVFHKAFSRTALERGWLRLFLLRMNGRPIGGLYGFRRGGRFYFYQSGYD
                     PAWRKYSVGLVTMGLAIKHALAEGAEEYDLLHGTEAYKFRWAKETRDLSRVHLFPPSW
                     RGWLCRAS"
BASE COUNT          779 a         1162 c         1316 g          834 t
ORIGIN      
        1 cgattacgcg gcgatcgtcc ggcaacgacg gcgaaacttt tcgctgttag ccgaacttct
       61 ggcggaatcc ggcatcgctc catggcgagc gctcgaagcc ggtgtctgtc ccttgttttt
      121 cccgttgctg gtcaaagaca agcgggcggc atcgcgaaag ttgaaagaga gcggtgtcat
      181 cgcgaccgaa ctgtggaacg aaggcgaccc actgtcggcg gcgcacgaag gctcgggagc
      241 caaattcttg aggcggcatc tgctcgagtt gccgattcac caggatgtgg atgaggcgca
      301 gatccgatat atggccgcac aagtgtgtga aggagagatc gcgctggagt cagcagtgaa
      361 cgcgtcgctt cgtctggcag gcggccgccg catccgaagc acccatcaac cgatcgcatc
      421 ttcagggtaa gggaggcact atgtcacaag aggccacctc gcttgaatct cggcatgagg
      481 tcgagcgtcg ttttcacgat gaacgcatgg cgggtcgcca ggccgacgac cgggattttt
      541 atgcggtcgg cggcatggac ctggtgctca atgcctatca tgaagccatg ggcgacgtgt
      601 gcggaaaaac cgtcctggac ttcggttgtg gggatggagc gcgttcgctc gagtatgcga
      661 agcgcggggc gacggtgtac gcgttcgaca tttcaccgga aagcgtgcgc aatctcgtcg
      721 ttacagctca gagcgccgga ttggccgacc gaatccatgc ctcggtcatg ccggcggaga
      781 cgctggccta tcccgacggg atgttcgatc tcgtgctagg cgtctccatt ctccatcata
      841 cggacgtctc gatggtcgag cgggaaatcg agcgcgtgct gaagccgggt ggtcgcgcac
      901 tcttcatcga accactgggg cataatccgt tccttcggct gttccggctg ctgacgccaa
      961 atcgccgcac gcccaccgag caacctatga ccgtcgggca gatccggcgt ctcattggac
     1021 gctttcggca aggccgattc cgcggatttc atttcctctc gatcgtaccg cccggcctgc
     1081 tctgggcgac cggcaatcgg gccctttttc aaacgatgat gcgcatgacg caaacagccg
     1141 accgttggtt gctcgcagcg gcaccaccgc tccggcgcta ctgctggatg acattaatcg
     1201 acgtgagcaa ataggagccg cgtattttta tttgttttat acagtcccgc cgggcacggc
     1261 tcggcacgag gagggcagag tcatggatat ggcaacgatc attccggctg caatgggctc
     1321 gtctcggaac ggctccatcg tgatggagcc gagggccgac acacagcgag tgagcctgcg
     1381 tgtcgacctc attgaggacg acgatacgtt cttgcggatg ggatcggtgt ggaaccggtt
     1441 gatcgacgaa gcggggatcg atcatccttt cctgcgccat gaatgggtcc gtacctggtg
     1501 ggagtgtttc aagcctgcgg gcaagctgca catcctggtc gtgaaggagg aggacgagcc
     1561 gattgccatt gcgcccctca tgttcgacca ggggcgggta tacgggtgct ccgtacggcg
     1621 cttacgcggg atcgccaacg tctatacgga gcggttcgat ctgattttga ccaggcgggc
     1681 gaaggaagct tgcagggcga tctggcagtt cctttccgcc gaagcttctc agtgggatat
     1741 gttggagctg cggcaggtcc ccgaaaacgc ccgcgcggcc gaatatctgc ggttctgggc
     1801 gtatgaagac aacttcctgc tcgggcaatg gcactcatcg gacgggccgt tcgttccgat
     1861 cgatcagccg tgggacagct atatcaagac gctgtcgaaa aagcatttgt ccaacctccg
     1921 cggccgcgcc aaaggcctgc accggatcgg agacgtgcgc catgaaatcg tcacgggggg
     1981 cgaacagttg ggacgcaccc tggacgaagc attcttgctg gaagctgccg cgtggaaggg
     2041 caaagccggc accgccatca tgaaccggcc cgatcgcctg gcgttctatc gcagcctgct
     2101 gacgagggcc gccgagggcg gatggctgcg attgcacttc ctgacgatcg agggaaaacg
     2161 gatcgccgtc cagatcgcgc tcgagataca caataagctc tacattttaa aatccggcta
     2221 tgatccgcac tatgcgccct ttgcgccgtc gctgctgctc tgcgagtgga tgctgcggga
     2281 cgcatggaaa cgcaaactca gcgaggttga ttttctcggc gacgccgaac ggtggaagct
     2341 ggaatggacg agtcagacga gagcgcactc ctgggtgttc attttcccga accgcatgcg
     2401 cactcgatgg ctgcatcgct tcaaattcag tttgctgccg cgcctctacg aacatccctt
     2461 gtatcaggtc gtccgatcgg ccggtcgacg gctcggccta cgtttgcacg actgaagctg
     2521 gcatgccgga gtatttggga cgatgaacgc gttggcaatc catcgggtcg gtcactggtg
     2581 ctaccagcgc cgaattccgc tcattccgca ggtggcctat ggtgccatat tcctgctgta
     2641 tcgggccgtg attcccatgt cggtggaatt cggcgaaggc acggaactgg cctacggggg
     2701 gctgggcatc gtgttgcacg agcgcacaaa gatcgggcgg tacgtgtcga tcggacatga
     2761 cgtgacgatc ggagggcgct cccgccgatg gggcgtgccg gtgatcgccg atcgctgtgt
     2821 gattggcgca ggggcgaagt tactggggcc gatctcggtc ggcgaggcgt ccgtgatcgg
     2881 cgccaatgcc gtcgtcctgg acgatgtgcc gcccaacagc gtgatggtcg gcctgccggc
     2941 tcaggtcgtc cggcagggca tcgacattca tgactacagc tgcctccaac cgccggagga
     3001 ggccgatcgc ctgtccttcc gcacgtcgaa taacttattg atcacgatca tcgaggacgc
     3061 cggaaggctc tcgcatctcg tcgatgaatg gaccgacctg ctggaacata gcgagcagga
     3121 ttgtctgttc ctcacgcccg aatggctcac gacctggtgg cgctgcttcg aacgcgacga
     3181 atggacgctt cgtctggtca ccgtccgacg ccaaagccag ctgctgggca ttgcgccgtt
     3241 cttctcgcgt cagcggagat tcggtggatt cctgagccat caatccaccg agtttctcgg
     3301 aaccggcgtt gtcggctccg attatctgga cctcattgtc agacgcggag aagaggacat
     3361 ggtgcgggtg ggcttgacgg attatttcaa ccgctcgaag ccgttgatga ctctcagtca
     3421 tctgccggcg cgtgccgcgg aagccgacgg catggtcaag gaactggagt tgagcggttg
     3481 gcaagtcagg cgcagcacga tcgagagctg tccgtacatc acgctgcgcg gccacacctg
     3541 ggagtcctat ctggattcgc tcggcgcgaa tcaccgctat aactttacgc ggcggctgaa
     3601 gaacctgcgc aaggcaggag tgctcgagtt cgatgtggtg gaaaaagagg atcagcgccc
     3661 cgaggccttg caggcattcc tgtcgcttca cgcggtcgcc tggcgcgaac gaggcggctc
     3721 ccaggcgatg cagacggcac gagaactggt gtttcacaaa gcgttcagcc ggaccgccct
     3781 tgaacgcgga tggcttcgcc tgttcctgct gcgcatgaac gggcgtccta ttggcgggct
     3841 gtacggtttc cggagggggg gccggtttta tttttaccag tccgggtatg accccgcctg
     3901 gaggaagtac agcgtcggat tggtgacgat gggccttgcg atcaaacatg cgctggcgga
     3961 aggggcagag gaatacgatc tgctccacgg gacggaagcg tataagttcc gctgggccaa
     4021 agaaacacgg gacttgtcgc gggtgcatct gtttccacct tcctggcggg gatggctgtg
     4081 tcgcgcctcc t
//
LOCUS       DASUOM010000306         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1614998,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000306 DASUOM010000000
VERSION     DASUOM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614998"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VFY27_06495"
     CDS             <1..1228
                     /locus_tag="VFY27_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5765198.1"
                     /translation="SLAAEEVKDRLRRQIPDLTDDEFARWDEHGLLERQLIDGRLLYF
                     NRSPSNLFRLSAEARARREVQTPLRDGPMEILNAHHYEVRQAALSQEAAGVLPQRIRV
                     TQSLTVDADAVPEGETVRAWIPLPRVLPGHQEEFRIVRSEPGSREVAPEDVMQRTVYF
                     EKPALANAPTEFSVTYELTVLAQYTEINPDEVMPAPVTPELAPFVAERPPHIVFNKSL
                     RLFSEQIVGAEKNPWRIAQKLFAAVDEIPWAGAREYSTITNISDYALHAGHADCGQQT
                     LLLIALLRMNGIPARWQSGMVYSDGDYWNLHDWGLFYLEPYGWLPMDVTFGLFEGADE
                     LEWFYLGGLDAYRIAFNDDFGTQFSPPKQHFRSETVDSQRGEAEWDGGNLYFDQWDYG
                     FDAQLVLPAKSAGASR"
     gene            1270..>2856
                     /locus_tag="VFY27_06500"
     CDS             1270..>2856
                     /locus_tag="VFY27_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765199.1"
                     /translation="MKAKRLQRLALSISMALPLSLCLTPVAPVALAQSQDGAVAGRTE
                     PGAELVIRNPDTGFSRTTTATADGSFRFPFIPVGTYSLEATKEGEPIASAIPVTVSLG
                     KTTIVDAGVIDEIQVVASRSINAIDVSSTESATNINKAQLARLPVERDLLSVAQLAAG
                     VNKGEFDGVSFGGSSVAENAVYINGLNVTDFYRRIGFSSVPYAFYEEFQVKTGGYSVE
                     FGRTTGGVINAVTRSGSNEFEYGVEAVWEPSFLQGDGDDHYDADGNPIYIASHDEYDQ
                     QSLNLYAGGPILRDKLFFFAMYEARNYEPTNTNNAGSLINEEQSDDGFWGAKIDWQIN
                     DNNLLEFLAFSDENTAATDVYQFDLQSGTRGDFQNVEYEDNGGLNWSTTYTGYLTDAL
                     SMRAMYGETEREAAQYTPNDIDCNRIRDFRTVGTGDIGCTSSSSVIARTDNREQARLD
                     FQWSLGDHLVRFGVDHEVNTSEHSQFYPGPDRLVYEINDTAPGSTLANGGVVPAGVTA
                     YVRTRTNEVDGEFETTATAFY"
BASE COUNT          592 a          900 c          856 g          508 t
ORIGIN      
        1 ttcgctggcc gcagaagagg tgaaagaccg cctgcgccgg cagatccccg acctgaccga
       61 cgatgagttc gcacgctggg acgagcacgg cctgctggag cgccagctga tcgacggccg
      121 actcctttat ttcaaccgct cgccttccaa cctgtttcgc ctgagcgcgg aagcccgcgc
      181 gcgccgggag gtgcagacgc cgctcaggga cgggccgatg gagatcctga atgcgcatca
      241 ctatgaagtg cgacaggccg cattgtcgca ggaggcagcg ggcgtgctgc cgcagcgaat
      301 ccgggtaacg cagtcactga cggtggatgc cgatgccgtt cccgagggcg aaaccgtgcg
      361 cgcgtggatt ccgctgccgc gcgtgctgcc cggtcaccag gaggagttcc ggatcgtgag
      421 gagcgagcct ggcagccgcg aggtagcacc ggaagacgtc atgcaacgca ccgtctactt
      481 cgagaagccg gcactggcga acgcgccgac ggagttctcc gtcacctacg agctcaccgt
      541 gctcgcgcag tacaccgaga taaacccgga cgaggtgatg ccggcacccg tcacgccgga
      601 actcgcgccg ttcgtcgccg agcgtccgcc gcacatcgtc ttcaacaagt ccctgcgact
      661 gttttcggaa cagatcgtcg gtgcggaaaa gaacccgtgg cgcatcgcgc aaaaactgtt
      721 cgcggccgtc gatgaaattc cctgggcggg cgcgcgcgag tactcgacca tcacgaatat
      781 cagcgactac gcgctgcacg ccgggcatgc cgactgcggc cagcagaccc tgctcctgat
      841 tgccctgctg cggatgaacg gcataccggc ccgctggcag tccggcatgg tgtactcgga
      901 cggcgactat tggaacctgc acgactgggg actcttttac ctggagccct acggatggct
      961 gcccatggac gtaaccttcg ggctcttcga gggtgcagat gaactggagt ggttctacct
     1021 gggcggactg gatgcttatc gcattgcctt caacgacgat ttcggcacgc agttctcgcc
     1081 cccgaaacaa cactttcgct ccgaaaccgt cgactcgcag cgaggcgaag ccgagtggga
     1141 cggcggcaac ctgtatttcg accagtggga ttacggattc gacgcgcaac tcgtgttgcc
     1201 ggcaaagtcc gccggcgcaa gccgatagct ttagacgata actattagaa gacctttccc
     1261 ggaggggata tgaaagccaa aagactacaa cgactagcac tttccatttc catggcgctg
     1321 cccctgtcgc tatgcctgac gccggtcgca ccggtcgccc tggcacagtc gcaggacggc
     1381 gcggtggccg gacgcacgga accgggtgca gaactggtca tccgcaatcc ggacaccggc
     1441 ttcagccgca ccacgacggc gaccgccgac ggcagcttcc gctttccatt tatcccggtc
     1501 ggaacctatt ccctggaagc caccaaggaa ggcgagccga tcgcctcagc cattcctgtg
     1561 acagtcagtc tcggcaagac gacaatagtc gacgccggcg ttatcgacga aatacaggtg
     1621 gttgcatcgc gatcgatcaa cgccatcgac gtgtcttcta ccgaatccgc gaccaacatc
     1681 aacaaggcgc agctggcgcg acttccggtc gagcgggact tgctgtcggt ggcgcaactg
     1741 gcggcgggcg tcaacaaggg agaattcgac ggcgtctcgt tcggcggctc gtccgtcgcc
     1801 gagaacgcgg tgtacatcaa cggcctcaac gtgaccgact tttatcggcg catcggcttc
     1861 tcgtcagttc cgtacgcttt ctacgaggag ttccaggtca agaccggcgg ttattccgtg
     1921 gaattcggcc gcacgacagg cggcgtgatc aatgccgtca cgcgctccgg cagcaacgaa
     1981 ttcgaatatg gcgtcgaggc ggtgtgggaa ccttccttcc tgcagggcga cggcgacgac
     2041 cattacgatg ccgacggcaa cccgatctac atcgcaagcc acgacgaata cgaccagcaa
     2101 agcctcaacc tctatgccgg cggcccgatc ctgcgcgaca agctgttctt tttcgcgatg
     2161 tacgaagccc gcaactacga gccgacgaac acgaacaacg cgggcagcct gattaacgaa
     2221 gagcagtcgg acgacgggtt ctggggtgcg aagatcgact ggcaaatcaa cgacaacaac
     2281 ctgctcgaat tcctcgcttt ctctgacgag aatactgcgg caacggatgt ctaccagttc
     2341 gatctccaga gcggtacccg cggcgatttc cagaacgtgg agtatgagga taacggcggc
     2401 ctcaactggt cgacaaccta tacggggtac ctgaccgacg cgctttccat gcgtgcgatg
     2461 tacggcgaga ccgagcggga agccgcccag tacacgccga acgacatcga ctgtaatcgt
     2521 atccgggact tccggacggt tggcaccgga gacatcggct gcacgtccag ttcgtctgtc
     2581 attgcccgga cggataaccg tgagcaggcg agactggatt tccagtggtc tctcggggac
     2641 cacctcgtcc ggttcggcgt ggatcacgaa gtcaatacgt cggagcactc acagttctat
     2701 cccggcccag accggctggt ctacgagatc aacgacaccg cccccggttc gactcttgca
     2761 aatggcggcg tcgttcctgc cggagtgact gcatacgtcc gtacccgcac caacgaggtc
     2821 gatggcgaat tcgaaaccac cgcaaccgcg ttttat
//
LOCUS       DASUOM010000307         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1699088,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000307 DASUOM010000000
VERSION     DASUOM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699088"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFY27_06505"
     CDS             complement(<1..260)
                     /locus_tag="VFY27_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5765200.1"
                     /translation="MAIKCGIVGLPNVGKSTLFNALTAAEIPAENYPFCTIEPNVGVV
                     PVPDPRLAVLSEIVKPQKTLPTTMEFVDIAGLVAGASKGEGLG"
     gene            complement(250..837)
                     /gene="pth"
                     /locus_tag="VFY27_06510"
     CDS             complement(250..837)
                     /gene="pth"
                     /locus_tag="VFY27_06510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5765201.1"
                     /translation="MNMHLSIIAGLGNPDDRYERTLHNAGFWFADAVAAMGRGQFRYE
                     KKFDAEVCKIDLAGGELRVVKPQSYMNLSGGPIRAVLDYYQLDPVKLLVAHDEIDLPP
                     GVARLKKGGGHGGHNGVRDTIRHCGNDFMRLRLGVGHPGTKDKVTGYVLKQGPAEVEE
                     SVREAIAQALDVLPLLAESGLAIAMNRLHTKSDGD"
     gene            complement(918..1580)
                     /locus_tag="VFY27_06515"
     CDS             complement(918..1580)
                     /locus_tag="VFY27_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5765202.1"
                     /translation="MKQEFNLVAELREDQGRGASRRLRRQGKVPAIIYGGGRPPRALV
                     FDHNKVLHQLDHEAFYSSILSIKVGDKSQAAIIKDVQRHPAKRQIMHMDIQRIVEDEK
                     IRMNVPIHIVGEQQAPGVKLGGGTVMRLITEVEVSCLPRDLPEYFEVDISHLELDQML
                     YMSDIKVPEGVEIIELSHGEEHDDAIVSVHLMKSVAVEEEAPAEEAVPALEQGKSEPE
                     QD"
     gene            complement(1843..>2163)
                     /gene="prs"
                     /locus_tag="VFY27_06520"
     CDS             complement(1843..>2163)
                     /gene="prs"
                     /locus_tag="VFY27_06520"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX5765203.1"
                     /translation="DVKGKTCIIIDDMVDTAGTLCQAAGALKKAGAEKVVAYITHPVL
                     SGPAVSRITGSELDEIVVTDTIPLRPDAEACPKIRQLSTAELLAETMRRISDEESVSS
                     LYVD"
BASE COUNT          374 a          692 c          648 g          449 t
ORIGIN      
        1 ccgagcccct cgcctttcga tgcgcccgcc accaggcccg cgatgtcgac gaactccatg
       61 gtcgtcggca gcgttttctg cggcttcacg atttcgctca agacggcgag ccgcggatct
      121 ggcacgggca cgaccccgac gttcggttcg atcgtgcaga acgggtagtt ctccgccggg
      181 atctccgcgg cagtgagcgc attgaaaagg gtcgacttgc cgacgttggg caagccgacg
      241 ataccgcact taatcgccat cggacttcgt gtgcaggcgg ttcattgcga tggcaagtcc
      301 gctctcagcc agcagaggca ggacgtcgag cgcctgcgct atggcttcgc gaaccgattc
      361 ctccacctcc gcaggtccct gcttgagtac gtacccggtg accttgtcct tggtaccagg
      421 atgccctacc cctaagcgca ggcgcatgaa atcgttgccg cagtgcctga tcgtgtcgcg
      481 gacgccgttg tggcctccgt gaccgccgcc cttcttcagg cgagccactc ccggcggaag
      541 atcgatttcg tcgtgcgcca ccagcagctt gacgggatcc agctggtagt agtcgaggac
      601 tgccctgatc gggccgccac tcaggttcat gtagctttgc ggcttgacga cccggagctc
      661 gccacccgcc aggtcgatct tgcagacttc ggcatcgaat ttcttctcat agcggaactg
      721 cccgcggccc atcgcggcaa ccgcgtccgc gaaccagaag cccgcgttgt gcagcgtgcg
      781 ctcgtagcgg tcatccgggt tgccgaggcc ggcaatgatg ctcaagtgca tgttcatgga
      841 cgttcagggc aattaatcag gtaaacaaaa aggccgccag cggcggcctt atcgctggct
      901 catcagggga aggaatccta gtcttgctct ggctcgctct ttccctgttc cagcgccggt
      961 accgcctctt ctgccggtgc ttcttcctcg accgcgaccg acttcatcaa gtggaccgac
     1021 acgatcgcgt cgtcgtgttc ctcgccgtgc gacagctcga tgatctcgac gccttccggc
     1081 accttgatat ccgacatgta cagcatctgg tccagttcca ggtgcgagat gtcgacctcg
     1141 aaatattccg gaaggtcgcg cggcaggcag ctcacttcca cctcggtgat gagacgcatc
     1201 acggtaccgc cgccgagttt cacgcccggc gcctgttgct cgccgacgat gtggatcggc
     1261 acgttcatgc ggatcttctc gtcctcgacg atacgctgga tgtccatgtg catgatctgg
     1321 cgcttggccg gatgacgctg aacgtccttg atgattgcgg cctggctctt gtcgccgacc
     1381 ttgatcgaca ggatgctgga gtaaaacgct tcgtggtcga gctggtgcag caccttgttg
     1441 tggtcgaaca ccagcgcgcg cggcgggcgt ccgccgccgt aaatgatcgc aggaactttt
     1501 ccctggcgac gcaggcggcg gctcgcaccc ctcccctggt cttcacggag ttccgcaacc
     1561 agattgaatt cctgcttcat gaggatctct ccgattggta agtagctgat ttcctgacgt
     1621 tatgccgtcc ggatgcaaaa tgccgggcgc gaccacccgg aaccggcgcg catcatagcg
     1681 ctaagccgcg caaatgtcac agcggacatg gccccggggg gccgagaggc ccgcgagtcc
     1741 ccttgtagga gcctgccatg caggcgcgtg cgatatgcat cagcgaactc gcctgcatgg
     1801 caggctccta caggcgcact gatcacgaag catgcaggtg cactaatcga cgtacagcga
     1861 actgacagac tcctcgtcgc tgatgcgccg catggtttcg gccagcagtt ccgccgtcga
     1921 gagctggcgg attttcgggc aggcctcggc gtcgggccga agcggaatcg tgtcggtgac
     1981 tacgatctca tcgagttcgg atccggtaat tcgggagacg gcaggccccg aaagcaccgg
     2041 gtgcgtgatg taggccacca ccttttcggc gcccgccttt ttcagggcgc ctgcggcctg
     2101 gcaaagcgtg cctgcggtat ccaccatgtc gtcgatgatg atgcaggtct ttcccttcac
     2161 gtc
//
LOCUS       DASUOM010000308         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1551638,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000308 DASUOM010000000
VERSION     DASUOM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551638"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFY27_06525"
     CDS             <1..306
                     /locus_tag="VFY27_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765204.1"
                     /translation="AASDLRKAARQEDELTYGEPPEWSVPVRQELGLVLLKAGRAADA
                     ERAFREDLKRFPENVWSLQGVAKALHAQRRTSEAEQVSKRLQQVWTMAGVKPGQAGL"
     gene            complement(400..714)
                     /locus_tag="VFY27_06530"
     CDS             complement(400..714)
                     /locus_tag="VFY27_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765205.1"
                     /translation="MKLLIAGLAAGLLSTVAPAWADDDRRDGWRGHTTQQVRHWDNRW
                     DRHRYSHRHHPRAPQRHWHRYDDRRYYYAPPRQRHYQRDYRYGYYAPEPSPSVWFSWT
                     IR"
     gene            909..1502
                     /locus_tag="VFY27_06535"
     CDS             909..1502
                     /locus_tag="VFY27_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX5765206.1"
                     /translation="MARRRKVTNLEQLLDRITEAARGRNRVSLGGILEVVGRRSFGPL
                     LLFAGLIAVSPVIGDTPGVPTAVGLFVLLVTGQLLFGREYVWLPRWLLERSVAQDKLC
                     KALGWLRKPARFVDRLLRPRMTALTHTIGTYVIAAVCVVIAGAMPVMEVVPFVANGAG
                     AALTAFGLALVAHDGLLAILAFGFTAITLGFVLQGIL"
     gene            1595..2029
                     /locus_tag="VFY27_06540"
     CDS             1595..2029
                     /locus_tag="VFY27_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5765207.1"
                     /translation="MRFMIVVKATQESEAGVMPDEKLISDMAAYHEELQKAGMLLDGS
                     GLQPSSKGWRIRYSGGKRTVVDGPFTETKDLIAGYTIIQARSREEALEWTKRFPNPAI
                     GGRDCEIEVRQLFELEDFEPVLGMKSEAIERFREMGIGTMKP"
BASE COUNT          471 a          740 c          830 g          485 t
ORIGIN      
        1 gccgccagcg acctgcgcaa ggccgcacga caggaagacg aactcaccta cggcgagccg
       61 ccggagtggt ccgtgccggt acggcaggag ctgggtctgg tgttgctcaa ggcgggccgc
      121 gctgccgatg cggagcgggc ctttcgcgaa gacctgaagc gttttccgga gaacgtgtgg
      181 tcactgcagg gcgtcgccaa ggcgcttcat gcccagcgcc ggaccagcga agcggaacag
      241 gtcagcaaac gactgcagca ggtctggacg atggcggggg tgaagccagg ccaggccggc
      301 ctgtaatcat cctcgacaaa acagcccgcc gggtcacaag ccggcgggct gtttcattgg
      361 ttgcgctgcc gctgcaagcg cctatgcatc gcgactttgt tatctgatcg tccacgaaaa
      421 ccagacgctc ggggaaggtt cgggtgcgta atacccgtag cggtaatccc gttggtaatg
      481 acgctgccgt ggaggcgcgt agtaatagcg gcggtcatcg tagcgatgcc agtgcctctg
      541 cggcgcgcgc gggtgatgcc gatgcgaata gcggtgccgg tcccagcggt tgtcccagtg
      601 ccgtacctgc tgggtcgtat gtccgcgcca gccgtctcgc cggtcgtcgt cggcccatgc
      661 cggcgccacc gtgctgagca gtcccgcagc cagtccagcg atcaagagtt tcattgccag
      721 tcctttcagt ttggtccgtg tccattcggt tatgggtttt tctttatcgt tagactaccg
      781 atatgccggg atttgtttgt gaggcgcccg taaaatcctg taaatatttg tttcattgat
      841 tcgcgggtgc aacgaatgct taccaaacgg agcacgaaac ctgacggcgg catgcgggtc
      901 tacgatatat ggctcgtcgc cgcaaggtca cgaatctgga gcagctgctc gaccggatta
      961 ccgaagctgc gcgtggtcgc aatcgggtct cactgggcgg gatactcgag gtggtcggac
     1021 gccgctcgtt cggcccgttg ctgctgtttg ccggactgat cgcggtttcg cctgtcatag
     1081 gcgacacgcc cggtgtcccg accgcggtcg ggctgtttgt gctgctcgta acgggacagc
     1141 tgctgttcgg acgcgagtat gtgtggctgc cgcgctggct gctcgagcgt tcggtcgcgc
     1201 aggacaagct ctgcaaggca ctcggttggc tgcggaagcc ggcccgcttc gtggatcgac
     1261 tgctgcgacc acggatgacg gcgctgacgc ataccatcgg cacttacgtc atcgcggcag
     1321 tgtgcgtcgt catcgcggga gccatgccgg tgatggaggt ggtgccattc gtggcgaacg
     1381 gggcaggagc cgcgctcacc gccttcggcc tcgccctcgt cgcgcacgac ggcctgctcg
     1441 caatcctcgc tttcggcttt accgcgatca cgctcggctt cgtcctgcaa ggcatactct
     1501 gattcggcag tgtcgatacc gagctttccc gttcgactaa gcggagaacg gtgtcgttgg
     1561 gatgccggtt acaactcgag aaggagaccg gagcatgcga ttcatgatcg tcgtcaaggc
     1621 aacgcaggag tccgaagcgg gcgtgatgcc ggacgagaag ctcatttcgg acatggctgc
     1681 ctaccacgag gaactgcaga aggccggcat gctgctcgac gggtcgggat tgcagccgag
     1741 ctcgaagggc tggcgcatca ggtatagcgg cgggaagcgc acagtcgtcg acgggccttt
     1801 caccgaaacc aaggacctga ttgcgggcta cacgatcatc caggcgaggt cgcgtgaaga
     1861 agcgctcgaa tggacgaagc gctttcccaa tcccgccatc ggcggcaggg attgcgagat
     1921 agaagtcagg cagctgttcg agctggaaga tttcgagccc gtgctcggca tgaagtcgga
     1981 agcgatcgag cgcttccgcg agatgggtat cggcaccatg aaaccctgaa ctgaacactc
     2041 aatgcagaag cctgccgaca aggatgctgc gcgatcgggt cgggtgatga agactctgct
     2101 ccagatgaag gagctcgata tcgcgctgtt cgcaggtcta agagggtaga taccgccttt
     2161 tgcgagcact acccggatgt gcccgctgaa accaagcgtt acacaccgga aaaaatcctg
     2221 aagcgggcgc tcggttatat tggatcccga aaggaacgtt cgcagatgct tccgacttac
     2281 atgttgtggt gcccttccac cggactttcg cgcgcgcggc cggaaggcac tcagggcacg
     2341 aacggcgact cgcgtggcgg ccggacaaag cgtccggctt cccggagtgc cgcccgccgc
     2401 acaaacgcga ctgccgtgct cccttccaga agatagggcc agtgtccgcc ggcggtggcc
     2461 ggagccatcc gccagcgaag cgcggactac ctgattccga gcttgtggtc caacgcgagc
     2521 ggacct
//
LOCUS       DASUOM010000309         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1609118,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000309 DASUOM010000000
VERSION     DASUOM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609118"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="VFY27_06545"
     tRNA            complement(1..76)
                     /locus_tag="VFY27_06545"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Glu,seq:ctc)
     gene            160..1485
                     /gene="pcnB"
                     /locus_tag="VFY27_06550"
     CDS             160..1485
                     /gene="pcnB"
                     /locus_tag="VFY27_06550"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX5765208.1"
                     /translation="MKNSGIRNAIEPTIIPRAAHNVSRAEISKNALKVLYRLHNAGFQ
                     AFLVGGSVRDALLGLHPKDFDIATDAHPEEVKALFGNCRLIGRRFRLAHVRFGREIIE
                     VATFRAASTLEDDPDVAHDLEGRILRDNVYGKIEEDVWRRDFTCNALYYNIADFSIWD
                     YVGGVEDIRRRRLALIGDPDKRLREDPVRMLRAVRFAAKLDFRIDKAVADAIAQRRGL
                     LRNVPPARLFDEFLKLFQSGHARKTFDLLREHKLFRELFPETEEALEREEDFLRFVKA
                     ALENTDHRVAEGQSVTPMFLLGVFLWAPVQRHAKRLQEKEKLSDTLALGLAAEDIVAI
                     QQSRVSMPRRFTSPMREMLALQPRFENRQGRRGIKFLEHRRFRAAYDLMMLRAEVGKV
                     DRESADFWTDVQTQNEEQRMVSFGLAGKGKSGRGRRRPRRRRPPQANSP"
     gene            1482..1991
                     /gene="folK"
                     /locus_tag="VFY27_06555"
     CDS             1482..1991
                     /gene="folK"
                     /locus_tag="VFY27_06555"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5765209.1"
                     /translation="MTSGRDHWRPAYVGIGSNLDDPASQVRKAFAALQRLPQSGFARC
                     SPFYRSAPMGPSDQPDFVNAVAVILTKLGPHELLRELQAIEQAHGRRREARQWGPRIV
                     DLDLLLMGQVVIEEPGLQIPHPGIAQRNFVLFPLADIAPGLLVPGIGSVQKLVAALGN
                     TSPRIERMD"
     gene            2000..2674
                     /locus_tag="VFY27_06560"
     CDS             2000..2674
                     /locus_tag="VFY27_06560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5765210.1"
                     /translation="MISERNREAGAAPRFIVVEGPIGVGKTSLAKRLAESFGGEVVLE
                     HVEQNPFLERFYRNRKDAALPAQLFFLFQRARQLEELRQSDLFSPVRVADFHFAKDRL
                     FAEINLDREEMSLYEQINAKLKVDVPVPDLVIYLQASIDTLLQRIMRRGIHYERAIER
                     HYLEKLAEAYARYFHSYDEGPLLIVNASSIDPVHNDTDYEQLYRQIRRTSGGRHFFNP
                     AAAALA"
     gene            2674..3504
                     /gene="panB"
                     /locus_tag="VFY27_06565"
     CDS             2674..3504
                     /gene="panB"
                     /locus_tag="VFY27_06565"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5765211.1"
                     /translation="MYTQLEQRGMAEPPVNIATLRQMKLEGSPIACLTAYDASFAALV
                     DAAGTDLVLVGDSLGMVIQGHDTTVPVTVADVIYHTRNVARGLRRAFLVADMPFMSYT
                     TPEQALGNAVQLMQDGGAMMIKLEGGEGQVEIVQHLAKHDIPVCAHLGLKPQSVHKIG
                     GFKVQGREPKAAKRMVDSARRLQDAGADIVLLECVPNEVGSAVTRALEVPVIGIGAGP
                     HVDGQILVLYDILDVTPGKKPRFVKNFMAGLDAPLAALTAYVTAVKDRSYPEPQHCFS
                     "
     gene            3557..>4291
                     /gene="panC"
                     /locus_tag="VFY27_06570"
     CDS             3557..>4291
                     /gene="panC"
                     /locus_tag="VFY27_06570"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5765212.1"
                     /translation="MHVEKTAGALRERLHEWRRKGDHVALVPTMGNLHDGHLSLITLA
                     REHAERIVVSVFVNPTQFEAGADFENYPRTLATDKRKLKRANADILFVPEVDTMYPFG
                     IEGATTVSVPKYRDDLCGAFRPGHFDGVTTVVSRLFGLVSPDVAVFGQKDFQQQLVIR
                     RMVDDLHWPVKIVTGETVREESGLAYSSRNQYLTGEERQVAPGLYSMLRQVGRELQSG
                     RRNFSELESQAMATLSGQGFSPEYVAI"
BASE COUNT          842 a         1310 c         1366 g          773 t
ORIGIN      
        1 tggtactccc taccggattc gaaccggtgt taccgccgtg agagggcggc gtcctgaacc
       61 actagacgaa gggagcactt gagggtcttt cgaatctgcc ccggtcgggc gcaggctgct
      121 attatacggc cccattacca gaatccaagt cgaatctatt tgaaaaacag cgggatccgg
      181 aacgccatcg aaccaacaat cattccccgc gcggcccata acgtctcccg ggccgaaata
      241 tcgaagaacg ccttgaaagt cctgtaccgg ctgcacaacg ccggattcca ggcctttctg
      301 gtgggcggca gtgtgcgcga cgcgctcctc ggtctgcatc ccaaggactt cgacatcgcg
      361 acggacgcgc acccggaaga ggtcaaggcc ttgttcggca actgccggct tatcgggcgg
      421 cgttttcgcc tggcacacgt tcgctttgga cgggagatca tcgaagtggc gacgtttcgc
      481 gccgcctcga cgctggaaga cgatcccgac gtcgcacacg acctcgaagg ccgtattctt
      541 cgcgacaacg tctacggcaa gatcgaagaa gacgtctggc gccgggattt cacctgcaat
      601 gcgctctact acaacattgc cgacttcagc atctgggatt acgttggcgg cgtcgaggac
      661 atccgccgca ggcggctggc actgattggc gaccccgaca agcgcctgcg tgaagacccg
      721 gtgcggatgc tgcgggcggt gcgcttcgcc gcaaagctcg actttcgcat cgacaaggcg
      781 gtcgccgatg cgatcgcgca acgccgcggc ctgctcagga acgttccgcc ggcacgcctg
      841 ttcgatgaat tcctgaagct cttccagtcg ggccacgcgc ggaaaacttt cgacttgctg
      901 cgcgagcaca agctgttccg ggaattattc ccggagacgg aagaagcact ggagagagag
      961 gaagatttcc tccgattcgt gaaggcggct ctcgaaaaca cagaccaccg tgttgccgag
     1021 gggcagtccg tgaccccgat gttcctcctc ggcgttttcc tgtgggcgcc ggtgcagcgc
     1081 catgcaaagc gcctgcagga aaaggagaag ctttcggata cgctggcatt ggggcttgcc
     1141 gccgaggaca tcgtggcgat acagcagtcg cgggtttcca tgccgcgccg tttcaccagc
     1201 ccgatgcgcg agatgctggc gttgcagccg cgcttcgaga accgccaggg ccggcgcggc
     1261 atcaagttcc tggagcaccg ccgtttccgc gccgcctacg acctgatgat gctgcgcgcc
     1321 gaggttggta aagtcgatcg tgagagcgcc gacttctgga cagacgtaca gacgcagaac
     1381 gaagaacagc gaatggtgag cttcgggctc gcgggcaagg ggaagagcgg cagaggcagg
     1441 cggcgcccgc gacggcgtcg cccgccacag gcaaacagtc cgtgacctcc ggccgggacc
     1501 actggcggcc cgcctatgtc ggcatcggca gcaacctgga cgacccggcg tcgcaagtcc
     1561 ggaaggcgtt cgcggcgttg caaaggctgc cgcagtcagg cttcgcccgc tgttcgccgt
     1621 tctatcgctc cgcgccgatg gggccgtcgg accagccgga tttcgtgaac gcagttgccg
     1681 tgatccttac gaagctcggc ccgcacgaac tgctgcgcga gctgcaggca atcgaacagg
     1741 cacatggccg gcgacgcgag gcacggcagt ggggaccgcg cattgtcgac ctcgacctgc
     1801 tgctcatggg ccaggtggtt attgaagagc cgggtcttca gattccgcac ccgggcatcg
     1861 cgcagcgaaa ttttgtattg ttcccgctcg ccgacattgc ccccgggctg ctggtgccgg
     1921 gcatcggcag cgtgcagaaa ctcgtagcgg cgcttggcaa taccagcccg cggatcgaac
     1981 gcatggactg aaggctcagg tgatatcaga acgaaacagg gaagccggcg ccgcgccgcg
     2041 attcatcgtt gtcgaaggcc ccatcggcgt cggcaagacc agccttgcga aacgccttgc
     2101 cgaaagtttc ggcggtgagg tcgtgctcga acacgtggag caaaatccgt tcctggagcg
     2161 cttctatcgc aatcgcaagg atgccgcgct tcccgcgcag cttttttttc tgttccagcg
     2221 ggcgcgtcag ctcgaggagc tgcgacagtc ggacctgttt tcgcccgtcc gcgttgccga
     2281 ttttcacttc gcgaaagatc gcctgttcgc ggagataaac ctggaccggg aggagatgtc
     2341 cctgtacgag caaatcaacg cgaagctcaa agtggatgta ccggtaccgg acctggtgat
     2401 ctacctgcag gcttcgatag atactctgtt gcagcgaatc atgcgacgcg gcatccatta
     2461 tgagcgggcg atcgagcggc attacctgga gaagctggcc gaagcatatg ctcgttactt
     2521 ccacagctac gacgagggac cgctgctgat cgtcaacgca tcgtctatcg atccggtaca
     2581 caacgacaca gactacgagc agctttaccg gcagattcgc cgcacgtccg gcgggcgcca
     2641 cttcttcaat cctgctgccg cggccctggc ctgatgtaca cccagctgga acagcgcggc
     2701 atggccgaac cgccggtgaa tatcgcaacc ttgcgccaga tgaagctgga aggcagcccg
     2761 attgcctgcc ttactgccta cgacgccagc ttcgccgcgc tcgtcgatgc tgcgggaacg
     2821 gatctcgtgc tggttggcga ctcgctggga atggtgatac agggccacga tacgacggtt
     2881 cccgttaccg ttgccgacgt gatctaccac acgcgcaacg tcgctcgcgg tttgcgccgc
     2941 gcattcctgg tggcggacat gccgttcatg agttacacca cgccggaaca ggcgctggga
     3001 aacgccgtgc aactgatgca ggacggcggc gcgatgatga tcaaactgga aggcggcgaa
     3061 ggccaggtcg agatcgtgca gcatcttgcg aagcacgaca tcccggtatg cgcgcacctc
     3121 ggactgaaac cgcagtcggt gcacaagatc ggcgggttca aggtgcaggg cagggagccg
     3181 aaagccgcga agcgaatggt tgacagtgcg cggcggctgc aggatgccgg cgccgacatc
     3241 gtcctgctcg aatgcgtgcc caacgaggtt ggcagcgctg tgacacgcgc gcttgaagtt
     3301 ccggtcatcg gcatcggtgc gggtccgcac gtcgacgggc agattctcgt cctgtacgac
     3361 atcctggacg tcacgccggg caaaaagccg cgcttcgtga agaatttcat ggccgggctc
     3421 gatgcgccgc tcgccgcgct gacggcctac gtcacggccg tcaaggatcg ttcttacccg
     3481 gagccgcagc attgtttttc ctgagtgggc tcacaagcgc gggagtcgat ctgcacaggc
     3541 tggcgccggg tcccccatgc acgtagaaaa aacggccggc gcactgcggg aacggctgca
     3601 cgagtggcgt cgcaagggag accacgtcgc gctcgtaccg accatgggca acctccacga
     3661 cggtcacctt agccttatca ccctggctcg cgagcacgcg gaaagaatcg tcgtgtctgt
     3721 tttcgtcaac ccgacacagt ttgaagcggg agccgacttc gagaactatc cgcgcacgct
     3781 tgccaccgac aagagaaagc tcaagcgcgc gaatgcggac atcctgttcg tccccgaggt
     3841 ggacacgatg tatccgttcg gcatcgaggg cgcgacgacc gtttcggtgc cgaaatatcg
     3901 cgacgatctc tgcggcgcgt tccggcctgg gcacttcgac ggtgtgacca cggtggtaag
     3961 ccggctgttc ggcctcgtgt cgccggacgt ggcggtgttc ggacagaagg atttccagca
     4021 acagctggtc atccggcgaa tggtggacga cctgcactgg ccggtgaaga tcgtcacggg
     4081 ggagacggtc cgcgaagaaa gcgggttagc ctacagctcg cgcaaccaat acctgaccgg
     4141 cgaggaacgg caggtggcgc ccggcctgta ttcgatgttg cgacaggtcg gccgcgagct
     4201 gcaatccggg cgcaggaatt tctcggagct tgaaagccag gcgatggcaa cactgtcggg
     4261 acaggggttt tcaccggagt atgttgcgat c
//
LOCUS       DASUOM010000310         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1693862,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000310 DASUOM010000000
VERSION     DASUOM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693862"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1072
                     /gene="sohB"
                     /locus_tag="VFY27_06575"
     CDS             50..1072
                     /gene="sohB"
                     /locus_tag="VFY27_06575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEX5765213.1"
                     /translation="MTEFFAEYGLFLLKAITVAGAMVFVIAAAATISRKAGSQEGLEV
                     ESLNRKYRSHAQALQKVVLGKSRWKAVAKADKARDKAAAKSKGERPRTFVIDFKGDLK
                     ASAVQSLREEVSAVLAVAGEGDEVVVRLENYGGVVHEHGLGASQLARIRDRNIPIVVT
                     VDKVAASGGYLMACVANRIVAAPFAILGSIGVIAQLPNFNRMLESHGVDFEQVTAGEY
                     KRNVTMFGKNTDADRAKLKEQLEDVHYLFKAAVASYRPNLDLAKVATGEHWYGARAVE
                     LGLADELKTSDALLAELAADRDLYSVRYKVRKPLQKRLMANVDDVLEKVDSAGWRRRF
                     ETRLPR"
     gene            1176..2000
                     /locus_tag="VFY27_06580"
     CDS             1176..2000
                     /locus_tag="VFY27_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5765214.1"
                     /translation="MNNLKAGSKAGSILLGTCLAIVLAACASSPTGRSQLVLKSDAAL
                     AAEGQRQFNMIREQMPLVKDRATIDYVACVANAIVDELEGEDAEMFWELAVIDQPDVN
                     AFVLPGGKISVFSGILTVAQNQHQLAAVLGHEVAHVTAQHANERASRGSVANVGIDIA
                     AILLGGGYARQTQAAQQTLRTGAALGIMNPFSRKQETEADIIGLEYMAKAGFDPRESV
                     TLWKNMAEKNESKIPEFMSTHPSGDTRIESLVSQFPTSLAYYNEAQAKGQSPNCSR"
     gene            2020..>2320
                     /locus_tag="VFY27_06585"
     CDS             2020..>2320
                     /locus_tag="VFY27_06585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5765215.1"
                     /translation="MISGTSSSFKDHFSSHAHRYAAFRPTYPQALMKFLADCCEKRGL
                     AWDCATGNGQAAIALAPFFDRIIATDASEAQICSAAPHPRIDYRVARAEDSGLDQS"
BASE COUNT          480 a          717 c          747 g          376 t
ORIGIN      
        1 gaacaatgtc ggcatcgctg tctgaagacg tgacggcgcc gggcacgcca tgactgaatt
       61 cttcgccgag tacggcctgt ttctgctgaa ggcgatcacg gttgccggcg cgatggtatt
      121 tgtcattgcc gccgccgcga caataagccg taaagccgga agccaggaag gcctggaagt
      181 agagagcctg aaccggaaat accggtccca cgcacaggcg ctgcaaaagg tcgtgctggg
      241 caagagccgc tggaaagccg tggcgaaagc cgacaaggcc agggacaagg cggccgcgaa
      301 atccaaaggc gagcggccgc gcaccttcgt cattgacttc aagggcgacc tgaaagccag
      361 cgccgtgcag tccctgcgcg aggaggtgag cgccgtgctg gccgtcgccg gcgagggcga
      421 cgaggtggtt gtccggctgg agaattacgg cggcgtggtg cacgagcacg gccttggcgc
      481 ctcgcagctc gcgcgcatcc gcgatcgcaa tatcccgatc gtggtgacgg ttgacaaggt
      541 tgcggcgagc ggaggctacc tcatggcctg tgtcgcgaac aggatcgtcg ccgcgccgtt
      601 cgctatcctg ggttccatcg gagtgatcgc gcaattgcct aatttcaacc ggatgctgga
      661 aagccatggc gtcgacttcg agcaggtcac cgcaggggaa tacaaacgca acgtcacgat
      721 gttcggcaag aacaccgatg cggaccgggc gaagctcaag gaacaactcg aggacgtgca
      781 ttacctgttc aaagctgccg tggcctctta tcggccgaac ctcgaccttg cgaaggtcgc
      841 gaccggagaa cactggtacg gcgcccgggc ggtggagctg ggcctggccg acgagctcaa
      901 gaccagcgac gcgctgctcg cggaactggc ggccgaccgc gacctgtaca gcgttcggta
      961 caaggtccgc aaacccctgc aaaaacgcct tatggcgaac gtcgacgacg tactggagaa
     1021 ggtagactcg gccggctggc gccgccgctt cgaaacccgg ctgccgcgct gagatcgtct
     1081 ctgcctgcaa ggcagccggc accgggaacc cgccgcaggt cgcaatgtct gagagggtaa
     1141 gtcggctata atgccgtctt tgcgggagga ggctaatgaa caacctgaag gccgggagta
     1201 aagcgggcag catcctgctc ggaacctgcc ttgctatcgt actggccgcc tgtgcgtcat
     1261 cgcccaccgg ccgcagccag ctcgtgctga agtcggacgc ggcgctggcg gcggaaggcc
     1321 agcggcaatt caacatgatt cgcgaacaaa tgccgctggt gaaggatcgc gcgacgatcg
     1381 attacgtcgc ctgcgtcgcc aacgcgatcg tcgacgaact ggaaggcgaa gacgcagaga
     1441 tgttctggga actcgccgtc atcgaccaac cggatgtgaa cgcgttcgtg ctgcccggcg
     1501 gcaagatcag cgtattcagc ggcatcctga ccgtcgcgca gaaccagcac cagctcgccg
     1561 cggtgctcgg ccatgaggtc gcccacgtca cggcgcagca tgcgaatgag cgcgcttcgc
     1621 gcggctccgt cgcgaacgtg ggcatcgata ttgccgcgat tctattgggc ggcggttatg
     1681 cacgacagac gcaggcggcg cagcagacgc tccgcaccgg cgcggcgctc ggcatcatga
     1741 atccgttcag ccgcaagcag gaaaccgagg ccgacatcat cgggctcgaa tacatggcga
     1801 aagcaggttt cgatccgcgt gaaagcgtca cgctgtggaa gaacatggcg gagaagaacg
     1861 aaagcaagat tccggagttc atgtcgacgc acccttccgg cgatacgcgt atcgaatcac
     1921 tggtcagcca gttcccgact tcgctcgcct attacaacga ggcgcaggca aaggggcagt
     1981 caccgaactg cagccgctga cagcgcaacc gacgacaagg tgataagcgg aacgtcgtcg
     2041 tccttcaagg accatttctc gtcgcacgcg catcgctacg cggcctttcg gccgacctat
     2101 ccgcaggcgc tgatgaaatt tctcgccgac tgttgcgaga agcgagggct tgcctgggat
     2161 tgcgccacgg gcaacggcca ggctgccatc gcgctggccc cgttcttcga tcgcatcatc
     2221 gccacggacg cgagtgaagc gcagatctgc tccgccgccc cgcatccccg aatcgactac
     2281 cgcgtcgccc gcgccgagga ttccggtctc gaccagtctt
//
LOCUS       DASUOM010000311         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1429231,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000311 DASUOM010000000
VERSION     DASUOM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429231"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2463)
                     /locus_tag="VFY27_06590"
     CDS             complement(172..2463)
                     /locus_tag="VFY27_06590"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX5765216.1"
                     /translation="MSDPLEQSSLRYHSLPTPGKLEIASTKPLSNQLDLAQAYSPGVA
                     YPCLRIKSQPDEAALYTTRGNLVAVITNGTAVLGLGNIGPLAAKPVMEGKAVIFKRFA
                     GIDVFDLEIDESDPDMLVEVIARLEPTFGGINLEDIKAPECFEVERKLRARMNIPVFH
                     DDQHGTAIVSAAAIRNGLRLVNKRIEEITLVSTGGGAASLACLDLLVRIGLPPANITL
                     VDLHGVVYEGRTEDMNPYKARYARATRLRTLSEAIEGADIFLGLSAPDVLSADMVRKM
                     AERPLILALANPTPEILPEIAREARPDAIMATGRSDYPNQVNNVLCFPFLFRGALDVG
                     ATTVNHEMQIACVDAIADLALRESSYEVASVYQGEALRFGPEYLIPKPFDQRLMANVS
                     FAVAKAAMDSGVAARPIADLEAYRQKLKSFSHRTALFMQPVIDVAKRGRQRLAYAEGE
                     NESVLRTVQSAVDEGIADPIVIGRAAVVANKIRALGLRLREGTDFELVDPHEDPRYRE
                     YWQFYHSRVARKGVSVEAAKEAMRTDNTVIAACLVALEDADAMICGTVGRFDRHLRHI
                     IDIIGTESAGRRISSMSVLILPQGPLFIADTHIGIDPTAEQIVGTTLACAERISDFGI
                     RPRVALLSHSNFGSSRALAAKKMRLAVQMLRERAPGLELDGEMHASAALSKAVRDTIN
                     PFSTLEAAANLLIMPDLDTANISMELTRAVHGALLIGPILSGTAKPAHIVIPTATVKG
                     IFNMSALAVSDAWRQKNDTRQST"
     gene            complement(2497..3276)
                     /locus_tag="VFY27_06595"
     CDS             complement(2497..3276)
                     /locus_tag="VFY27_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5765217.1"
                     /translation="METNVIWLGFVGSLLAGLCTGIGASAIFLVRRLSDRTEDVLLSS
                     AAGVMLAATFFSLLLPAIGYGEDLYASRALAVAVVVGGFILGAAALHLIHRFVPHEHF
                     LAGREGPDTATLSRTWLFVIAITLHNFPEGMAVGVGFAGGDTGNGIALATGIGMQNIP
                     EGLAVAASMLAIRYTKTQAFLVACLTGLVEPIGGLVGSVAVSVAVPLMPWTLGFAAGA
                     MLFIISDEIIPETHRGGYETLATFSMLGGFSVMMFLDATLG"
     gene            3429..4502
                     /locus_tag="VFY27_06600"
     CDS             3429..4502
                     /locus_tag="VFY27_06600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5765218.1"
                     /translation="MPKFDDETALSRTVASTATLKLANLHSLDPFYTPLEERFERITR
                     LARRALNVPIAAITVVQGDRQWFKSVSGWQVTELPMSKSLCAEVIREARQMVVPDTLE
                     DLYMMSNPVVCKAPKIRFYAGFPIRDSEGKTVGTFCVMDLKPRKPDPDFSTTLADLGD
                     MAQRELFTVELSNAQSALVAKLGEARRQAMFDPLTRLWNRRGATDLLNAALKDAQKYN
                     HSVGICLADIDNFKQVNDQFGHQVGDQVLRRVAGSIVASVRPQDIVCRWGGEEFLVIV
                     HDVDETGCMAIGERICEGMRSLPIQTRDGSVPASISIGIAMRNRGNCIAPEQLVALAD
                     AALYQSKREGRDRVTVARAKPRA"
     gene            complement(4577..>5193)
                     /locus_tag="VFY27_06605"
     CDS             complement(4577..>5193)
                     /locus_tag="VFY27_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5765219.1"
                     /translation="AAVVLLGCTQPAPGNTGVYMLLDTSGTYREELQKAEQIIRYTLS
                     RLDASDTFAVARIDTGSFSEKDIVAKTSFDDRPSTINRQKRLFAKQISTFVSDTDSSP
                     YTDITGGLLQAVEFLNEKGPGRKTILIFSDLKEDLQEGYVRDDITFDLNGFEVIALNV
                     TKLRSDNVDPREYLSRLNAWQTRVEKSGGQWRVINDLDGLEELL"
BASE COUNT          989 a         1597 c         1596 g         1011 t
ORIGIN      
        1 ggtggttctg gggcatcgtg cacatctatt tcctgattgg cgtcaggagc aggttactcg
       61 tcgccatcca gtggctctgg tcctacctga cgttcgatcg aggcgcgaga ctcataaccc
      121 cggatgcaca accggccgtg aaacgccggc cctcgccgga tcacccttag gtcaggtgga
      181 ctgccgtgtg tcgttcttct ggcgccaggc gtcggagacc gcaagcgcgc tcatgttgaa
      241 gatccccttg acggtcgccg tcggaattac tatgtgtgcc ggtttcgccg ttccggaaag
      301 gatcggtccg atcagaagcg cgccgtgaac cgcgcgtgtc agttccatgg atatgtttgc
      361 cgtgtccagg tcgggcatga tcagcaggtt ggcggccgcc tccagcgtcg agaaaggatt
      421 gatcgtatcg cggaccgcct tgctcagcgc cgccgatgca tgcatctcgc catccagttc
      481 caggccagga gcgcgctccc gcaacatctg caccgccagg cgcattttct tcgccgccag
      541 cgcgcgcgac gagccgaaat tggaatgcga cagcagcgct acgcgcggcc tgataccgaa
      601 gtcgctgatc cgctcggcgc aggcgagtgt ggtaccgacg atctgttctg ccgtcgggtc
      661 gatcccgatg tgcgtatccg ctatgaacag gggtccctgc ggcaggatca gcacgctcat
      721 cgaggaaatt ctccggccgg cagattccgt accgatgatg tcgatgatgt ggcgcagatg
      781 ccggtcgaac cgccccaccg tgccgcagat catcgcgtcg gcgtcctcca gcgcaacgag
      841 acaggccgct atgacggtat tgtcggtacg catcgcctcc ttcgctgctt cgaccgagac
      901 gccttttcgc gccacgcgtg agtggtagaa ctgccaatat tcccggtacc tcgggtcttc
      961 gtgtggatcc accagctcga aatcggtacc ttcgcgcagg cgcaatccga gcgcccggat
     1021 cttgttggca acgaccgccg cgcggccgat gacaatcgga tcggcgatac cctcgtccac
     1081 cgctgactgc accgtgcgca acacgctttc gttttcccct tctgcataag caagccgctg
     1141 gcgaccccgc ttggcaacat cgatgaccgg ttgcatgaag agagcagttc gatggctgaa
     1201 actcttcagc ttctgccggt acgcctcgag atcggcgatc ggccgcgcgg cgacgccgct
     1261 gtccatcgcg gctttcgcca cggcgaatga cacattcgcc atgagccgct ggtcgaacgg
     1321 cttcgggatc aggtactcgg gaccgaaccg cagcgcctcc ccctggtaga cgctggcgac
     1381 ttcgtagctc gattcgcgaa gcgccaggtc ggcgatggca tcgacgcagg ctatctgcat
     1441 ttcgtgattc accgtggtag cccccacgtc cagcgcgccg cggaacagga acgggaaaca
     1501 gagtacgttg ttcacctggt tcgggtaatc cgacctgccg gtggccatga tcgcgtccgg
     1561 acgagcctcg cgtgcgattt caggcaatat ctccggggtc ggattcgcca gggcaagaat
     1621 gagcggccgt tccgccatct ttcgcaccat atcggcgctc agcacgtcgg gcgcagagag
     1681 gccaagaaag atgtccgctc cttcgatagc ttcactcagc gtgcgcagcc tggtggcgcg
     1741 cgcataacgt gccttatacg gattcatatc ctccgtacgc ccttcgtaca cgacaccgtg
     1801 cagatccacc agtgtgatgt tggcgggcgg caacccgatg cgtacaagga ggtcgaggca
     1861 ggcgaggctc gcagcgccgc cgccggtcga cacgagtgtg atttcctcga ttctcttgtt
     1921 aaccagccgc aggccgttcc tgatcgcagc cgcggacacg atagcggtgc catgctggtc
     1981 gtcatggaag acgggaatgt tcatacgtgc gcggagcttc cgttccactt cgaagcactc
     2041 cggcgccttg atgtcttcca ggttgattcc gccgaacgtc ggttcgagcc gcgcgatcac
     2101 ttcgaccagc atgtccgggt cggactcgtc tatctccagg tcgaagacgt cgatgccggc
     2161 gaagcgtttg aagatcacgg ccttgccttc catcaccggt ttcgcagcca acggcccgat
     2221 gtttcccagg cccagcacgg cggtgccgtt ggtgattact gcgaccaggt tcccgcgtgt
     2281 tgtgtacagc gccgcttcgt cgggctgcga ctttatccgc aggcacgggt aagccactcc
     2341 cggggaatac gcctgggcca ggtcgagctg gttcgacaga ggcttcgtcg atgcgatttc
     2401 aagtttcccg ggcgtcggca ggctgtgata ccggagggaa ctttgctcga gtggatcaga
     2461 cattcgggta gcttaccgta tcggcgccga agcccgtcag ccgagcgtcg cgtcgaggaa
     2521 catcattacg gaaaagccgc cgagcatgga gaatgtcgcc agcgtttcgt aaccgccacg
     2581 atgcgtctcc gggatgatct cgtcactgat aatgaacaac atggcgccgg ccgcgaaacc
     2641 gagcgtccat ggcatcagcg gcaccgcgac cgaaacggcg acgcttccga ccaggccccc
     2701 gatcggctcg accagcccgg tcaggcaggc aacgaggaat gcctgggttt tcgtgtaacg
     2761 aatcgccagc atggacgccg cgacagccag tccctccggt atgttctgca tgccgattcc
     2821 cgttgccagt gcgatgccgt tgcctgtatc gccacccgcg aagccgacgc cgacggccat
     2881 gccttcaggg aagttgtgca aagtaatggc gatcacgaac agccaggtgc gactcaaggt
     2941 ggcggtgtcc gggccttcgc ggcccgccag gaaatgctcg tgcgggacga atcggtggat
     3001 gagatgaagg gcagctgcac cgaggataaa tcccccgaca accactgcga cggccagtgc
     3061 cctgctggcg tagaggtctt ccccatagcc gatcgccggc agcagcagcg agaagaacgt
     3121 cgcggcgagc atgactcccg ctgcggaact caagagcacg tcctcggtac ggtctgagag
     3181 ccgccgcacc aggaaaatcg ccgatgcccc gatcccggtg caaaggccgg cgaggaggct
     3241 gcccacgaag cccagccaga tgacgttcgt ttccaattca gatgctccgt gccgcgatgt
     3301 gaaccggttc acgcttccgc gaacgtgaac cagtagctgg cgccgtggga agtgattgca
     3361 ttaaaagata caggattcgt gttatctggc cacctgccgc cttacgccgg gtcaagagag
     3421 tagccgaaat gcccaagttc gacgatgaga cagcgttaag ccgtacagtg gcaagtacgg
     3481 caacgctgaa actcgcgaat ctgcattcgc tggacccctt ctacactccc ctggaagaac
     3541 ggttcgagcg cattacacgg ctcgcacggc gcgcgctgaa cgtgcctatt gcggccataa
     3601 cggtcgtaca gggggaccgg cagtggttca agtccgtttc tggatggcag gttacggagc
     3661 tgccgatgag caagagcctc tgtgccgagg ttatccggga agccagacag atggtcgtcc
     3721 cggacacgct tgaagatctt tacatgatga gcaatcccgt ggtctgcaaa gcgccgaaga
     3781 tccggttcta cgccggcttc ccgatacggg attccgaggg caagacagtc ggcacgttct
     3841 gcgtcatgga cctgaagccg cgcaagccgg atcccgactt cagcaccacg ctcgctgatc
     3901 tcggcgatat ggcgcaaagg gagctgttca cagttgagtt gagcaacgct cagtcggcat
     3961 tggtcgcgaa actgggcgaa gcgcggcgac aggcgatgtt cgatccgctg acccggctat
     4021 ggaaccggcg cggggccacc gatctcctga atgcggcgct gaaggatgcg cagaaataca
     4081 accactccgt cggcatctgc ctcgccgaca tcgacaactt caagcaggtc aacgaccagt
     4141 tcggccacca ggtcggcgac caggtgttgc gccgcgtggc agggtcgatc gttgcatcgg
     4201 tccgcccgca ggatatcgtc tgccgctggg gtggtgagga gtttctcgtc atcgtccacg
     4261 acgtcgatga aacgggctgc atggccatcg gcgagcgcat ctgcgagggc atgcgttcgc
     4321 tcccgataca gacccgcgac ggttcggttc ccgccagcat cagcatcggg atcgcgatgc
     4381 gtaaccgcgg aaattgcata gcgccggagc agctcgtcgc acttgccgac gcggcgctct
     4441 accagagcaa gcgtgaggga cgggatcgcg tgacggtcgc acgcgcgaaa cctcgagcct
     4501 gatcaacaac gacgacctgg gactctatcc gggcgagttc gctgaagcgc agtcacacct
     4561 gcacgcatgg gagctcctac agaagttcct cgaggccgtc gaggtcgttg atgacccgcc
     4621 attggccacc cgatttctca acccgcgtct gccacgcgtt gagcctcgac aggtactcgc
     4681 gcggatcgac attatcggag cgcagcttcg ttacgttcaa ggcgatcact tcgaagccat
     4741 ttaagtcgaa ggtaatgtcg tcgcgaacgt agccttcctg caggtcttcc ttgagatccg
     4801 aaaagatcag gatcgttttc cggcccgggc ctttttcgtt caggaactcg accgcctgca
     4861 acaaccctcc ggtgatatcg gtgtagggtg aagagtcggt atcgctgacg aatgtactga
     4921 tctgcttcgc gaacaggcgc ttctgtcggt tgatcgtgct ggggcggtcg tcgaaggaag
     4981 ttttggccac tatgtccttt tcgctgaagc ttccggtgtc gatccgggcc acggcgaagg
     5041 tgtccgacgc gtccagcctg gacagtgtgt agcggatgat ttgctccgcc ttttgcagct
     5101 cttcccggta tgtgccggat gtgtcgagca gcatgtagac gccggtgttg cccggtgccg
     5161 gttgagtgca gccgagcagc acgacggctg cga
//
LOCUS       DASUOM010000312         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1247537,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000312 DASUOM010000000
VERSION     DASUOM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247537"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFY27_06610"
     CDS             <1..392
                     /locus_tag="VFY27_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765220.1"
                     /translation="SARLEYEEIKRRMTGARLSERLEEENKGERFTLLKRAGLPTVPV
                     QPNRAAIISLGFVLAAGAAIGIAALVDALDSSIRSSRDLLSNFSMKPVGVIPYIRTAH
                     DRRMSWLKRSTAAGAAAISLVLVMALI"
     gene            411..1235
                     /locus_tag="VFY27_06615"
     CDS             411..1235
                     /locus_tag="VFY27_06615"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX5765221.1"
                     /translation="MSKIEDAIRKASLHHEGARRHAFDSQREADATDPASAEPLNRRA
                     PVLFRLQPGLKFQPAVDDLERNRIIHSGFPEDAVTGYKMLRTRVQQALSGNDWKTLAV
                     TAPHDGAGKTVTAVNLAITLASHGGHEIYLVDLDLRNPAIADCFGLPQDIPGLASYLD
                     GGRPIRELLWDVGVENLAVLASRDRMQNSSERITSKRMQDMISAFRTASPHPIVIFDL
                     PPVLVADDAVAIAPFVDGMLLVASEGETTREDFKGMLELLHHANIVGVVLNKSDLR"
     gene            1372..2502
                     /locus_tag="VFY27_06620"
     CDS             1372..2502
                     /locus_tag="VFY27_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765222.1"
                     /translation="MSVDDYAILTLVTALITVSAGLGQFGADSIVNRHVVAPDLRMFF
                     RVFCTSMIAALVTITAAVGYYGTGWPVALLLGFAIVCQSVVYFAAAYSRSQHNFRVSL
                     LVFNSFNYVLLAIAIVVSLQTRHGILYPLAAITCLLFMTAAWSLVHLRRKYSTISASY
                     SYNWREALVWTSITGSAVVLLQLERLITPKLLTLEDLATLGILLAIVGPPFRLLQLTL
                     GYVLLPKLRRAQSPRERSSLILREIWIAFSLLVPCWILTWYLVPVLDDLFFGAKYPLT
                     GNLVLAVIVASTAKALSGISTASVAGVASDRHLELSGLVSWAGVAVSLMAAYVGSGYG
                     LAGVVYGVSLGWFLRIVVAAFIVRKHFAADGQADGAVERSLP"
     gene            complement(2637..>2968)
                     /locus_tag="VFY27_06625"
     CDS             complement(2637..>2968)
                     /locus_tag="VFY27_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765223.1"
                     /translation="HEDTRSFIYIDDAVRATLMLALADKAANETVNVGSEQEVTILEV
                     GQQILEIEGINEKIELKDSPEGSVRRRAPDTSKLQALTGFAEEWSLTRGLKETIEFYL
                     RGSTVGV"
BASE COUNT          549 a          899 c          878 g          642 t
ORIGIN      
        1 acagcgcgcg cctcgagtat gaagaaataa agcgtcgaat gaccggcgcc aggctttccg
       61 aaaggctgga ggaggagaac aagggcgagc gcttcacgtt gctgaaacga gcagggttac
      121 cgacagtgcc ggtgcaacca aaccgagccg ctatcatcag cctcggcttc gtgctcgcgg
      181 ccggtgccgc aatcggcatc gcggcgctgg tggatgctct ggactccagc attcgcagca
      241 gccgcgattt gctgtccaac ttctcaatga aacccgtggg cgttattccg tatatacgta
      301 ctgcccatga cagacgcatg agctggctga agagatcaac ggcggcaggg gcggcggcta
      361 tatctctcgt cctggtcatg gccctgatct gaacggatga ggcatcaggg atgagcaaga
      421 tagaagacgc gatccgcaag gccagcctgc accacgaagg tgccaggcgg cacgccttcg
      481 acagccagcg ggaagccgat gctaccgacc ccgcatcagc ggagcccctg aacaggcgtg
      541 cgccggtgct tttccggctg cagcctggcc tcaagttcca accggcggta gatgatctcg
      601 agcgaaatcg catcatccat tccggctttc ccgaagatgc cgtaaccggc tacaagatgc
      661 ttcgcaccag ggtgcagcaa gcactcagtg gcaatgactg gaaaactctc gccgtcactg
      721 caccacatga cggagcggga aaaacggtta cggccgtcaa cctggccatc acgcttgcgt
      781 cccacggcgg acacgagatt tacctcgtgg acctcgacct gcgaaaccct gccatcgccg
      841 actgtttcgg actgccgcag gacattccag ggctcgcatc ctacctggac gggggcagac
      901 cgattcgcga actgctgtgg gacgtgggcg tcgagaacct ggcggtactc gccagcaggg
      961 atcgcatgca gaactcctcc gaacgaatca cctccaagcg catgcaggat atgatctcgg
     1021 cattcaggac cgcatcacct cacccgatcg tgatcttcga tcttcctcct gtacttgtgg
     1081 cggacgatgc cgtggcgatt gccccgtttg tcgacgggat gttgctggtg gcctctgagg
     1141 gcgaaacgac gcgggaggac ttcaagggaa tgctcgaact gctgcatcac gcaaacatcg
     1201 tgggagtggt tttgaacaag tccgacctgc gataggttcg ggcgccgccg agtcagggcg
     1261 ttgctaaacc tggggcgaag ggccctgcag tccgacaaat tcctcgcggg atccctgttc
     1321 gcaatctccg gccttgcctt ctccgctgca aacataattt tcgcgcgcgc gttgagcgtc
     1381 gatgattacg cgatactgac cctggtaaca gcgctcatca cggtatccgc aggactgggc
     1441 caattcggcg ccgacagcat cgtgaaccgt cacgttgtag cgccggatct gagaatgttc
     1501 ttccgggtct tctgtacatc catgatcgcg gcgcttgtga cgattaccgc cgccgtcggg
     1561 tactacggga ccggatggcc ggtagccctc ctgctcggct tcgcgatcgt ttgccagtcg
     1621 gtcgtttact ttgctgctgc atactcacgg tcacaacaca actttcgtgt ttccctgctc
     1681 gtcttcaaca gctttaatta cgtccttctc gctattgcga ttgtcgtgag tcttcagacg
     1741 cgacacggaa tactctatcc gctagcggca attacctgcc tgcttttcat gaccgctgcg
     1801 tggtccttgg tgcatctccg gcgcaaatac tcaaccatat cagcgagtta ctcctacaac
     1861 tggcgagagg cgctcgtctg gacatcgatc acgggcagcg ccgtggtgtt gctgcagctc
     1921 gagcggctta ttacacccaa gctcctgacg cttgaggatc tcgcgacatt gggcatcctg
     1981 ctggcaatcg tcggaccgcc cttccggctg ttgcagctga cactgggtta cgtattgctg
     2041 cccaagctcc ggcgcgcgca aagtccgcgc gaacggagct ctctcatcct gcgtgaaatc
     2101 tggatcgcat tttccctgct ggtcccatgc tggatactca cgtggtacct ggttccggtc
     2161 ctggatgatc tcttcttcgg ggcgaaatat cctttgaccg gtaaccttgt cctggctgtc
     2221 atcgtcgcca gtacggcgaa agcgctgagc ggcatcagta cggcgagcgt agcgggcgtg
     2281 gcgtcggaca ggcacctgga gctgtcgggg ctcgtgtcgt gggcgggagt agcggtctcg
     2341 ctgatggcag cctatgtcgg ctccggatac gggctcgccg gcgtcgttta cggggtaagc
     2401 ctggggtggt tcctgcggat cgttgtcgca gcgtttatcg tccgaaagca cttcgctgca
     2461 gatgggcagg cggacggtgc cgtcgagcgc tcgctgccgt agggagcgag gtgcagcgat
     2521 gacttcgaga ctctgcacaa gttgtcgttg tggcaccgta ggagcccgcc atgcgggcga
     2581 gttcgctgag ccatttcgca ctcgcccgca tggcgggctc cgacagcgct tgcaccctat
     2641 acgccgacgg tgctgccccg caggtaaaac tcgatcgttt ccttaaggcc gcgggtcagc
     2701 gaccactctt cggcaaaccc ggttaacgct tgcagcttgc tggtatccgg cgcgcggcgc
     2761 cgtacgctgc cttctggcga gtctttgagc tcgattttct catttatgcc ctcaatctcg
     2821 aggatttgct gcccgacctc gagtatcgtg acctcctgtt cgctgccgac gtttaccgtc
     2881 tcgttcgctg ccttgtctgc cagcgcaagc atcagagtgg cccgcacggc atcgtcgatg
     2941 tagatgaatg acctcgtgtc ttcatgtc
//
LOCUS       DASUOM010000313         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_198297,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000313 DASUOM010000000
VERSION     DASUOM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198297"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFY27_06630"
     CDS             complement(<1..443)
                     /locus_tag="VFY27_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5765224.1"
                     /translation="MIRVTENFRKIQDLLAKAAREAGRPEDSVRLLAVTKGQPAAAVI
                     EAVSAGQKSFGENFVQEAVGKIAEVGRDDLDWHFIGHLQSNKTRQVAEQFQWVHTVDR
                     LKVAERLSRQRPYYADDLNVCIQVMIDAEDDKSGVTPESLPELASA"
     gene            530..1570
                     /locus_tag="VFY27_06635"
     CDS             530..1570
                     /locus_tag="VFY27_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5765225.1"
                     /translation="MAVDVAQLLSFTVKNNASDLHLSGGVPPMIRVDGDIKRINMPAL
                     THKDVNSMVYDIMNDKQRKDYEEFLETDFSFEIPKLARFRVNAFNQNRGSAAVFRTIP
                     SEILSLDDLGAPQIFKDVSMHPRGIVLVTGPTGSGKSTTLAAMVDYINDNKPDHILTI
                     EDPIEFVHESKKSLINQREVHRDTLGFNEALRSALREDPDVILVGELRDLETIRLALT
                     AAETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKEMVRAMLSESLRAVVSQTLLKRIGG
                     GRIAAHEIMIGTPAIRNLIREGKIAQMYSAIQTGHSTGMQTLDQNLTELLHKGLINKE
                     EARFRAVNKENF"
     gene            1654..2811
                     /locus_tag="VFY27_06640"
     CDS             1654..2811
                     /locus_tag="VFY27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5765226.1"
                     /translation="MEREQAVRLTQNLLRKMVEKEGSDLFITTGFPPAIKVDGQVHKA
                     TDTPLTAEQSAMMVRSIMNDKQIREFDATKECNFAISPQGIGRFRVSAFIQQGMIGAV
                     LRTITTEIPTLGELDLPPILKDVVMNKRGLVVVVGGTGSGKSTTLAAMIGHRNENSKG
                     HIISIEDPVEYVHPHKGCVITQREVGVDTESWHAALKNTLRQAPDVILIGEIRDKETM
                     EYGIQFAETGHLCLATLHANSANQALDRIINFFPEERRQQLLMDLSLNTKAFISQRLI
                     PRESGVGRIAAMEIMLNSPLIADLIFKGEVAHIKEIMAKSTRLGMQTFDQALFALYED
                     GAISYEEAMRNADSKNELRLRVKLESKRDAAVADQQQESLNIMEEDTARTF"
     gene            2826..>3571
                     /locus_tag="VFY27_06645"
     CDS             2826..>3571
                     /locus_tag="VFY27_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5765227.1"
                     /translation="MNVKPLFKLMVEKRASDLFFAPYAPAKIKIDGKIMPVNKLEMTP
                     KMVKQAALELMDENQLDIFTRELEVDFAISEAGLGRFRVNVFHQRGNVAMVLRYITAD
                     MPVLDDLGMPEQLKDLVMLRRGLVLMVGASGNGKSTTLAAMINHRNEKTSSHIITVED
                     PIEFLHPNKQSIVNQREVGLDTKSYATALKSAMRAAPDVIQIGEIRDRESMQSAIDMA
                     GTGHLVLATLHSNNAPETLDRIINMFPQEQ"
BASE COUNT          810 a         1067 c         1035 g          659 t
ORIGIN      
        1 gcggaggcga gttccggcag gctctctggc gttacgccgg acttgtcgtc ttcggcatcg
       61 atcattacct gtatgcagac gttcaggtcg tcggcgtagt acgggcgttg ccggctcagg
      121 cgctctgcaa ccttcaagcg atcgacggta tgcacccact ggaactgctc ggcaacctgg
      181 cgggtcttgt tggactggag gtggccaatg aaatgccagt ccaggtcgtc cctgccgacc
      241 tctgcgattt tccccaccgc ctcctgcaca aagttttccc cgaacgattt ttgcccggcg
      301 gataccgcct cgatgacggc ggctgccggc tggcccttgg tgacggcaag caagcgtacg
      361 gagtcctccg gcctgccggc ttcacgtgcc gccttcgcca gcaaatcttg gatttttcga
      421 aagttttccg tgactctaat cacgttttgc taagggctct tcgctatact aggcagccat
      481 tggaccgggt tatggtaagt ccggcttctc aatctacaga gggagcaata tggccgtcga
      541 cgtcgcgcag ctactgtcgt tcaccgtcaa gaacaacgca tccgacctgc acctgtccgg
      601 cggcgtcccc cccatgattc gagtcgacgg cgacatcaag cgcatcaaca tgccggcgct
      661 gactcataaa gatgtcaata gcatggtgta cgacatcatg aatgacaagc agcgcaagga
      721 ctacgaagaa ttcctggaga cggacttttc cttcgagatc ccgaaactgg cgcgcttccg
      781 cgtcaacgca ttcaaccaga accgcggctc ggccgcggtt ttcaggacca tcccctccga
      841 gatcctttcg ctcgacgacc tgggagcgcc gcagatattc aaggatgtct cgatgcaccc
      901 gcgcggcatc gttctcgtta ccggccccac cggttcaggc aagtcgacga cgctcgcggc
      961 gatggtcgac tacatcaacg acaacaagcc ggatcacatc ctgacgatcg aagacccgat
     1021 cgagttcgtg cacgaaagca agaagtcgct catcaaccag cgcgaggtgc acagggacac
     1081 actgggcttc aacgaagcac tgcgttctgc gttgcgcgag gacccggacg taatactcgt
     1141 cggcgagctt cgcgacctgg aaacaattcg cctcgcattg accgctgccg aaacaggcca
     1201 cctggtcttc ggcacactgc ataccagttc ggccgccaag acgatcgatc gaatcgtcga
     1261 tgtattcccc gcggccgaga aggagatggt gcgagctatg ttgtccgaat cgctgcgcgc
     1321 cgtcgtttcg cagacgttgt tgaaacgcat cggcggcgga cgaattgccg cgcatgaaat
     1381 catgatcggt actccggcca tccgcaacct gattcgcgaa ggcaagatcg cccagatgta
     1441 ctccgcgata cagacaggcc actcgaccgg aatgcagacg ctcgaccaga acctgaccga
     1501 gctgctgcac aaagggctca tcaacaagga agaggccagg tttcgagcgg tcaacaagga
     1561 aaacttctag gcgaggcagc ggaaggttcg gcgggaatgt gaaagcagga tgaccgggcc
     1621 ggggcccggg gtcttacggg agaaggacga aaaatggaac gcgaacaagc ggtgcgcctt
     1681 acgcagaacc tcctgcgcaa gatggtggag aaggaaggtt ccgatctttt tattaccacg
     1741 ggattcccac cggcgataaa ggtcgacggc caggtgcaca aggccactga cacgccgctt
     1801 acggcggaac agtcggcaat gatggtccgc tccatcatga acgacaagca gatcagggag
     1861 ttcgacgcca ccaaggaatg taactttgcg atcagcccgc agggcatagg tcgctttcgc
     1921 gtcagcgcgt tcatccagca gggcatgatc ggcgccgttc tgcgcacgat cactaccgaa
     1981 atcccgacgc tcggggaact cgacctgccg ccgatcctga aagacgtcgt gatgaacaag
     2041 cgtggactcg tggtcgtcgt cggcggtacc ggctcgggca agtccacgac gcttgccgca
     2101 atgatcgggc accgcaatga gaattcgaaa ggtcacatca tctctatcga agacccggtc
     2161 gaatacgtcc atccgcacaa gggctgcgtc atcacccagc gggaagtcgg agtcgacacc
     2221 gaatcctggc atgcggcgct gaagaacacc ttgcgccagg cgccggacgt gatactgatc
     2281 ggcgagatca gggacaagga aaccatggaa tacggcatcc agttcgcgga gaccgggcat
     2341 ttgtgtctcg cgacactgca cgccaacagc gcgaaccagg cgctggaccg catcatcaac
     2401 tttttcccgg aagaacgcag gcagcagctt ctcatggacc tgtcgctcaa caccaaggct
     2461 ttcatctcgc agcgccttat tccgcgcgag agcggtgtag gccgcatcgc cgccatggag
     2521 atcatgctga actcgccgtt gatcgccgat ctcatcttca aaggagaggt ggcgcacatc
     2581 aaggagatca tggccaaatc gacacgcctc ggcatgcaga cgttcgacca ggcattgttc
     2641 gcgctgtatg aggacggcgc catctcctac gaggaggcca tgcgaaatgc cgattccaag
     2701 aacgagctgc gcctgcgggt caagctcgag agtaagcggg acgctgcggt cgcggaccag
     2761 cagcaggaaa gcctcaacat catggaagaa gacactgctc ggaccttctg agccggcggc
     2821 accctatgaa cgtaaaaccc ctctttaaat tgatggtaga gaagagagcg tcggatcttt
     2881 tcttcgcgcc ctatgctccg gcgaaaatca agatcgacgg caagatcatg ccggtaaaca
     2941 agctggagat gacgccgaag atggtgaagc aggcggcgct cgagctgatg gacgagaacc
     3001 agctcgatat cttcacccgc gaactggagg tggacttcgc catctccgaa gccgggctcg
     3061 gccgtttcag ggtcaatgtc ttccaccagc gcggcaatgt cgcgatggtg ttgcgttaca
     3121 ttacggcgga tatgccggtt ctcgacgacc tcggcatgcc cgagcagttg aaggacctgg
     3181 tgatgctgcg tcgcggcctg gttttgatgg tcggtgcaag cggcaacggc aagtccacga
     3241 cgcttgccgc catgatcaac caccgcaacg aaaaaacctc ctcgcatatc atcacggtcg
     3301 aggacccgat cgaattcctg catccgaaca agcagtcgat cgtgaaccag cgcgaggttg
     3361 gtctggatac gaagtcttac gccacggcct tgaagagcgc gatgcgcgcg gcacccgatg
     3421 tcatccagat cggcgagatc cgtgatcgcg agtccatgca gtcggcgatc gacatggcag
     3481 gaacgggcca cctggttctt gcgacgctgc actcgaacaa cgcgcccgag accctggacc
     3541 gcatcatcaa catgttcccg caggagcagc a
//
LOCUS       DASUOM010000314         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_395687,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000314 DASUOM010000000
VERSION     DASUOM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395687"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VFY27_06650"
     CDS             <1..1641
                     /locus_tag="VFY27_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765228.1"
                     /translation="GQRFSWLVETVQAQNDGFKKIDGNEDSSTGYDIEDYMVKLQLDS
                     DPSSAIYQSLRFKAGYTDQASDETYLGLTDEDFAIEPNRRYAASAGDRFESEHEQFQA
                     TYVFDPQANWRGEVTAYRNNFARNWFKLDSVNGADLGAVLDDPAAFPEEFAWLKGSDS
                     PEGAIVKRHNNREYYSQGIQAEATFDFGFGDTAMSVTTGVRVHEDEEDRLHRDDSFTM
                     EDSQLVLESSGAPGSAANRLGSAEARALFVDTEIRQGAWILTPGVRFEDIDMTRQDFA
                     STDPSRVSGPTQVRENSVSVSIPGIGALYRVSDNWRLLAGIHKGFNPPAPGSDASEED
                     SINVEFGTRYANDRFRFESIYFVNDYDNLVGTVTASTGGGGQIGDQFDGGQVTVQGVE
                     LTADYGWTNFGGRGLDLPLSLQYTWTTEAEFNNAFESDFEPWGDVQVGDELPYVPEHQ
                     LRAAAGLEKETWRINLAANYVGKMRTVAGQGEFIDNETVDSHVTWDLLARWHFTESLS
                     SYVKVENLLDETYVAARRPAGVRPGLERTAYVGLSFSL"
     gene            1736..1945
                     /locus_tag="VFY27_06655"
     CDS             1736..1945
                     /locus_tag="VFY27_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEX5765229.1"
                     /translation="MYICICNAVTDKQIRRAAASGVDNLYELREALGVASCCGSCADE
                     AESILREANSESTAGQPRLYIPSAA"
     gene            2219..2683
                     /gene="bfr"
                     /locus_tag="VFY27_06660"
     CDS             2219..2683
                     /gene="bfr"
                     /locus_tag="VFY27_06660"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5765230.1"
                     /translation="MKGDPTVIEHLNRVLKNELTAINQYFLHSRMLKNWGFHKLGDKE
                     YDESIDEMKHADEVIERILFLEGLPNLQDLGKLHIGEDVPELLQSDLDLELDALDDLQ
                     AAIAYCEAHKDYVSRQLFDKILKSEEEHVDWLETQLSVLEKIGLENYLQSQT"
     gene            complement(2826..>3036)
                     /locus_tag="VFY27_06665"
     CDS             complement(2826..>3036)
                     /locus_tag="VFY27_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5765231.1"
                     /translation="NAIAPGPFETKMMAHTLKTAGDKIIKRVPRKRIGQPADMAGAAI
                     FLSSMASSYVTGVVLPVDGGLTGTL"
BASE COUNT          655 a          949 c          891 g          541 t
ORIGIN      
        1 ggccagcggt tttcatggct tgtcgaaacg gtgcaggcgc aaaacgacgg cttcaagaaa
       61 atcgacggca acgaggactc gagcaccggc tacgacatcg aggactacat ggtcaagctg
      121 caactcgaca gcgacccgtc ctcggcaatt taccagagcc tgcgcttcaa ggcgggctac
      181 accgatcagg catcggacga aacctacctc gggctgacag acgaggactt tgccatcgag
      241 ccgaaccggc gctacgcagc ctccgccgga gaccgcttcg aaagcgagca cgaacagttc
      301 caggcgacct acgtcttcga tccccaggcg aactggcggg gcgaagtcac cgcgtaccgg
      361 aacaacttcg cgcgcaactg gttcaagctc gattccgtca acggcgcgga cctgggcgcc
      421 gtacttgatg atcctgcagc cttccccgag gagtttgcat ggctgaaagg aagcgatagt
      481 cccgaaggtg ccatcgtcaa gcgccacaac aaccgcgagt attactcgca gggcatacag
      541 gccgaagcaa cctttgattt tggcttcggc gacaccgcca tgagcgtgac gaccggtgtg
      601 cgcgtgcacg aagacgaaga agaccgcctg caccgcgacg acagcttcac gatggaagat
      661 tcgcaactcg tgctggagag ttcaggcgcg cctggttctg cggcaaatcg ccttggcagc
      721 gccgaggcgc gagccttgtt cgtcgatacc gaaattcggc aaggcgcgtg gatacttacc
      781 cccggtgtcc gcttcgaaga catcgacatg acgcggcagg acttcgccag caccgatccg
      841 tcgcgtgtct caggcccgac gcaggttcgc gagaattcgg tatcggtatc cattcccggg
      901 atcggcgcgc tctatcgggt aagcgacaac tggcggctcc tggccgggat acacaaggga
      961 ttcaaccctc cggcaccggg cagcgacgcg agcgaggaag acagcatcaa cgtggagttc
     1021 ggaacgcgct acgcgaacga caggttccgt ttcgagtcca tctacttcgt caacgattac
     1081 gacaacctcg tcgggacagt gaccgcgtct accggcggcg gcgggcagat cggcgaccag
     1141 ttcgacggcg gccaagtgac ggtgcagggg gtcgagctga cggccgatta cggctggacg
     1201 aattttgggg gccgcgggct cgatttgccg ctgagcctgc aatacacctg gaccaccgag
     1261 gcggaattca acaacgcctt cgaaagcgac ttcgaaccgt ggggcgacgt gcaggtggga
     1321 gacgaactgc cgtacgtgcc ggaacaccag ctgcgggcgg cggccggact cgagaaggag
     1381 acgtggcgaa tcaacctcgc tgcgaattac gtgggaaaga tgcggacggt tgccggccag
     1441 ggtgagttca tcgacaacga gaccgtcgat tcccacgtca cctgggacct gctcgcgcgc
     1501 tggcatttca cggagtcgct gagttcttac gtcaaggtcg agaacctgct cgacgaaacg
     1561 tatgtcgccg cgagacgccc ggccggagtg cgtccgggcc tggaacggac cgcgtacgtg
     1621 ggcctgagct ttagcctgtg aggcctgtca aatgtttgac gtaatgctat tgacaatcat
     1681 tcgcatttag gttggaatgc caccagatcc cgttcgccac tcaacttgtt gatttatgta
     1741 catctgcatc tgcaacgccg tcaccgacaa gcaaattcgc cgcgccgcgg cttccggcgt
     1801 ggataatctc tacgaacttc gggaggccct cggcgtagcc tcctgctgcg gcagctgtgc
     1861 cgacgaagcc gagtccatcc tcagggaggc caacagcgaa tcgaccgccg gccagcccag
     1921 gctctacatc ccctccgccg cctgaaacac cccgacctcc gaagagaccg ctgaaggagc
     1981 ccgtcccacg ggcgcaattc gctcggttcg acgcacctgt aggagcccgc catgcgggcg
     2041 aggtcgccaa cgcacgtcgc acttcgcccg catggcgggc tcctacacga cctcaaccac
     2101 ctcccatcca cacgcgaatc agactcattt ccccttccat tccccctaat tgttcttgcc
     2161 tccccggtca cgggtcttat gatgaacatc ccgtccacct ccctccacgg agaacttcat
     2221 gaaaggcgat ccgaccgtca tcgagcacct gaaccgcgtt ttaaaaaacg agctgacggc
     2281 gatcaaccag tacttcctgc attcgcgcat gctcaagaac tggggctttc acaagctggg
     2341 ggacaaggaa tacgacgaat ccatcgacga gatgaagcat gcggacgagg tgatcgagcg
     2401 cattcttttc ctcgagggac tgccgaacct gcaggatctc gggaagctgc acatcggcga
     2461 ggatgtcccg gagttactgc aaagcgacct cgacctggag ctggacgccc tggatgacct
     2521 gcaggctgcg attgcctact gcgaggcgca caaggactac gtcagccgcc agctgttcga
     2581 caagatcctg aagtcggaag aagagcacgt cgactggctg gagacgcagt tgtcggtgct
     2641 cgagaagatc gggttggaga attacctgca atcgcagacg taggcgagcg tgtaggaagc
     2701 cgccatgcgg gcgagttcgc tgtaggttaa tcgcactcgc ccgcattcgc cgtctgaatg
     2761 gcaagggttc gatatcgttg ccagggccgt tcggcgtggg ctcctacaaa acccccccgt
     2821 gaaaatcaca acgtcccggt aagcccaccg tcgaccggca acacaacccc ggtcacataa
     2881 ctcgacgcca tcgaggaaag aaatatcgcc gcacccgcca tgtccgcagg ctggccgatc
     2941 cttttccgcg gcacccgctt tataatcttg tcccctgctg tcttcagcgt gtgcgccatc
     3001 atcttcgtct cgaaaggccc cggggcaatc gcattg
//
LOCUS       DASUOM010000315         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_383884,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000315 DASUOM010000000
VERSION     DASUOM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383884"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /gene="glnE"
                     /locus_tag="VFY27_06670"
     CDS             <1..1786
                     /gene="glnE"
                     /locus_tag="VFY27_06670"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5765232.1"
                     /translation="GAVRIREAESLQNAYDFLRRLENAIQAIRDRQIHDLPEKPTDQA
                     RVALAMGYPSWTDLLDDLQERRQLVASAFAGVAFRGADAAPQAGTDDPFAALWSGAAD
                     VDEWEAALARLGYAESAELAAAVVAFSGSGGVAQLGKAARSRLRRFIPALLCAVRERN
                     QHAIVLKRVLGVIEHVIRRSAYVALLNENPVVLDRLVDLCETSAWLAEEIARYPLLLD
                     ELLDPRLYTAPLARENMLAELQRRLDSLSTTESEQQIEVLAQFQRAALFRIAVADVTG
                     NLPIMKVSDRLTELAEIVVTRALSIAWQDLAARHGRPVVRGEQGLRPAGFGVIAYGKF
                     GGMELSYRSDLDLVFLHDGDGSDQSTDGPKPLENSMFFVRLVRRVVHLLTTQTYSGAL
                     YEVDTRLRPSGRSGLLVTSVEAFERYQEENAWTWEHQALLRARPVAGSATVAREFERV
                     RSETLKERVRRDRLLTDVKEMRSKMRDQLDKSDAGRFDLKQGEGAIGDIEFLVQYLVL
                     ENARTHPAVIHYPDNIRQLGTLAAAGRLDADEVARLQDIYKRYRLRLHRLALDEKPPF
                     AGSGEFVEERQFVMTVWNRVMSAGSAKA"
     gene            1925..2146
                     /locus_tag="VFY27_06675"
     CDS             1925..2146
                     /locus_tag="VFY27_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5765233.1"
                     /translation="MATSKAGTIDQNLIPANAQHRYEVRYSDLPLSCPMPGMYLWNSH
                     PKVYLPIEETGEAMCPYCGAAYYLRKGSE"
     gene            2154..3092
                     /gene="rfaD"
                     /locus_tag="VFY27_06680"
     CDS             2154..3092
                     /gene="rfaD"
                     /locus_tag="VFY27_06680"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX5765234.1"
                     /translation="MHIVTGGAWFIGSNLVRRLLEETGDDVVVVDDLTDGHKFRNLAA
                     LPIADFLDKDEFLERLDPQFSSGVDCIFHQGACSTTTEWDGRYMMKNNYAYSRTLLHH
                     CLAHDIPLIYASSAAVYGSSPRFSESHENELPLNVYGWSKLLFDRYVQRLAEQASSQV
                     VGLRYFNVYGPNEQHKGSMASVAWHFNQQILTGDEIRLFEGSGGFEDGEQRRDFVYVD
                     DVCAVNLWFLQHRDASGVFNTGTGRSQSFNDVANAVIAWHGRGRIRYIPFPDELAGAY
                     QSFTEADLSKLRSAGCDVSFRNVEAGVRAYLDRLAG"
BASE COUNT          612 a          942 c         1027 g          605 t
ORIGIN      
        1 aggcgcggtc aggatcaggg aggcggagag tctgcagaac gcatacgatt tcctgcggcg
       61 gctggagaac gccatccagg cgatccgtga ccggcaaatt cacgacctgc ccgaaaagcc
      121 gacggaccag gcacgagtgg cgcttgccat ggggtacccg tcctggacgg atcttctgga
      181 cgacttgcag gagaggcgcc aactggtcgc aagcgctttc gccggagtgg cgtttcgcgg
      241 agccgacgca gcaccgcaag ccggaactga tgatcccttc gcggcactct ggagcggcgc
      301 ggccgacgtg gacgaatggg aagccgctct cgcgcggctg ggttacgcgg aaagcgccga
      361 actggcggca gcagtggtgg ccttctctgg cagcggaggc gtagcgcagt tgggaaaggc
      421 cgcacgttcc cgcttgcgcc gtttcatccc ggcgctgctt tgcgcagtca gagagaggaa
      481 tcaacacgcg atcgtgctga agagagtgct cggagttatt gagcacgtta ttcgccgcag
      541 cgcctatgtc gccttgctca acgagaaccc ggtcgtcctg gaccggctgg tcgatctttg
      601 cgaaacgagc gcatggcttg ccgaagaaat agcgcgttat ccacttctgc tggacgagct
      661 gctcgacccg cgcctgtata ccgcgccgct ggcgcgcgag aacatgctcg cggagctgca
      721 acgccggctg gacagccttt cgacgaccga aagcgagcaa cagatcgaag ttctcgcgca
      781 gttccagaga gccgcattat tccgcatcgc ggtcgctgac gtaaccggga acctgccgat
      841 catgaaggtc agcgaccgcc tgacggaact tgcagagata gtagtgaccc gggcgctgtc
      901 gatcgcatgg caggatcttg ctgcgcggca cgggcgcccg gtggtgcgcg gtgaacaggg
      961 gctgcgcccg gcgggattcg gcgtcattgc gtacggcaag tttggcggaa tggaactgtc
     1021 ttaccgttcg gatctcgatc tcgtgttcct gcacgatgga gacggttcgg accagagtac
     1081 ggacgggccg aaacccctgg agaacagcat gttttttgtc cggctggtca ggcgcgtagt
     1141 gcatctcctg accacccaga cttactccgg cgcgctgtac gaggtcgaca cgcgcctgcg
     1201 tccaagcggc cgctccggcc tgcttgttac gagcgtggag gccttcgagc gttaccagga
     1261 ggaaaacgcg tggacctggg agcatcaggc gctgctgcga gcgcgtcctg tcgccggtag
     1321 cgcaacggtg gcacgggaat tcgagcgggt gcggtcggaa accttgaaag agcgcgtgcg
     1381 ccgcgaccgc ctgctgaccg acgtgaagga gatgcggtca aagatgcgcg atcaactgga
     1441 caagagcgat gccgggcgct ttgacctgaa acagggggag ggagccatcg gggacataga
     1501 atttctcgtg cagtacctgg tacttgagaa tgcgcgcacg catcccgcgg tgattcatta
     1561 tccggacaac atccgccagc tcggcaccct cgctgcagcc ggccgcctcg atgcggacga
     1621 ggtggcgcgc ctgcaggaca tctacaagcg ctaccgtctg cggctgcacc gcctggctct
     1681 cgatgaaaag ccgccgtttg caggtagtgg cgaattcgtc gaggaacggc agttcgtaat
     1741 gacggtgtgg aaccgggtaa tgtctgcagg gtcggccaag gcttagatgc gggaaaaaac
     1801 ggttccgccg gatcctcgca ggttgcgccg ctgcccacac gtactcgccg attgcgaggc
     1861 cagggtcggg cgggaatccc gccttgctat aatgcagcgt tcctgttgat gagcggtgac
     1921 tgaagtggcg acgagcaagg ctggcaccat tgaccagaac ctcattcctg cgaatgccca
     1981 gcacaggtac gaggtgcggt attcggacct tccattgtcc tgcccgatgc ctggaatgta
     2041 cctgtggaat tcccatccga aggtctacct gccgatcgag gagaccggag aggcaatgtg
     2101 tccttactgc ggcgctgctt actacctccg caaaggctcc gagtaacacc cgcgtgcaca
     2161 tagtgaccgg cggcgcctgg ttcatcggca gcaatcttgt ccgccgcctg ctcgaggaga
     2221 ccggggacga tgtcgttgtt gtcgacgatc tcaccgacgg gcacaagttc cggaacctgg
     2281 ccgcgttgcc gatagcagac ttcctggaca aggacgagtt cctggagcgc ctggatccac
     2341 aattttccag cggcgtcgat tgcattttcc accagggcgc gtgttccacg acgacggagt
     2401 gggacggccg ctacatgatg aagaacaact acgcgtattc ccggacgctg ctgcatcact
     2461 gcctcgcgca cgacatcccc cttatctatg cctcgtccgc cgccgtctac ggcagctctc
     2521 cccggttcag cgagagtcat gagaacgagc tgccgctcaa cgtgtacggc tggtcgaagc
     2581 tgctgtttga ccggtacgtc cagcggctgg cggaacaggc ctcgtcgcag gtagtcggac
     2641 tgcgttactt caacgtgtat gggccaaacg aacagcacaa gggttcgatg gcgagtgtcg
     2701 catggcattt caaccaacag attcttacag gcgatgaaat ccggctcttt gaaggctcgg
     2761 gcggtttcga ggacggcgaa caaaggcgcg atttcgtcta cgtcgacgac gtatgcgcgg
     2821 tcaatctctg gttccttcag caccgcgatg cttcgggagt gttcaacacc ggcacagggc
     2881 ggtctcagag cttcaatgat gttgcgaatg ccgtgattgc ctggcacgga cgtggccgca
     2941 ttcgctacat tccttttccc gatgaactcg ccggcgccta ccagagcttc accgaggccg
     3001 acctgtcgaa gcttcgcagc gcgggctgcg atgtgagctt ccgcaacgtg gaagctggcg
     3061 ttcgcgcgta cctcgatcgg ctcgccggct aagcgcgttc aactgcacgg tggataagcc
     3121 ggccatcctg atcgtcggcc cgtcctgggt gggcgacatg gtcatggcgc aatcgctgtt
     3181 caagct
//
LOCUS       DASUOM010000316         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1617380,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000316 DASUOM010000000
VERSION     DASUOM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617380"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..262)
                     /locus_tag="VFY27_06685"
     CDS             complement(68..262)
                     /locus_tag="VFY27_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765235.1"
                     /translation="MNKKRDLDNIDMDESTYELLGSKLSKLAGTARGKERFRERRPND
                     RQESYAPAAKVKRMRDRPED"
     gene            complement(470..751)
                     /locus_tag="VFY27_06690"
     CDS             complement(470..751)
                     /locus_tag="VFY27_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765236.1"
                     /translation="MAKSVDTEPLPEWVEEDPEIELTMDMEDEDLEFDEFAEEGTVKE
                     VAEGPREHVPLWRLIEMSREDRSLKMELADFDDYDYDSTDNDYSGGLTH"
     gene            complement(930..2000)
                     /locus_tag="VFY27_06695"
     CDS             complement(930..2000)
                     /locus_tag="VFY27_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5765237.1"
                     /translation="MDTITGRNTSTAITLAAIVVIVYGMQAAKVLLVPFLLAVFLALI
                     TVRPMLWLQRHRVPSILAALLVLVLIMLVLAVAGTIVGASIAEFTAALPAYQARLDLL
                     VDRLLRLAATHMDNGGTIEGLSDIVDAGWAMRLVANILNALRDVLTNTFLILFTMVFI
                     LLEASSVQTKILAAFGRRAETFENATLFLNNLGRYLGIKTLISIATGLSAWLVTSIIG
                     LDFALLWGMLAFLLNYVPTIGSIIAAVPAVLLALVQLGIGEATATAIGFVVINVMFGV
                     ILEPRWMGYGVGISPLIVFTGLIFWGWIFGPVGMLLSVPLTMTLKLALESDNRTRWLA
                     ILIGSERDAEHELAQQDARQSA"
     gene            complement(2637..4097)
                     /gene="nhaC"
                     /locus_tag="VFY27_06700"
     CDS             complement(2637..4097)
                     /gene="nhaC"
                     /locus_tag="VFY27_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEX5765238.1"
                     /translation="MTTPKSPSLLDSIIPVISLILLLALSVYLFGSDAASGPTQIVLV
                     MGAAIAAIIAIRNGHKWAELQSAMIGGISTAMGAILILLAVGALIGTWLMSGTVPTLI
                     YYGLELLDPRFFFVATCLICAISSLATGSSWTTAGTLGVALIGVAMGLSLSPAIAAGA
                     VISGSYFGDKMSPLSDTTNLAPAVAGTDLFTHIRHMSLTTGPSFLIALVLYLVIGLGV
                     DVGSDLVQLRGLMATLDSSFNVTPLALIPLVVVFVFAMRKVPPLPTILFGALLGGVMA
                     VILQPQAVLEFSQGDGLPTGLAMTKGVWLALADGYVASTGVPAVDDLLTRGGMSSMLT
                     TIWLILAALAFGAVMEHAGMLARLIQGALRAAKSTGALITTVVLSCIGINVIAADQYI
                     AIVLPGKMFQAEFKRRNLDPKNLSRALEDSATLTSALVPWNTCGAYMAATLGVATFSY
                     APYAFLNLINPLISILYGVTGFSIKRLEPETQPSTA"
BASE COUNT          919 a         1382 c         1362 g          830 t
ORIGIN      
        1 ccggcgggcc atcggcgcca gatcgaaagc aacctgctcc ggaacaactg ccccggtgac
       61 cgccggatca atcctccggg cgatcccgca tccgcttgac tttagcggca ggcgcatagc
      121 tctcctgcct gtcgttcgga cgacgctcgc ggaagcgctc cttgccgcgg gcggtaccgg
      181 caagcttcga caacttgctg cccagcagct cgtatgtcga ttcgtccatg tctatgttgt
      241 ccaggtctcg tttcttgttc acgtccttcg cctcagatcc gagtcaaacg tcccgacgtg
      301 cgacgcgcca tgactcgcca aaaagcattg ccaaaaaagg attaccgatt ggtggattta
      361 aaagaaggta tttcccgcat gaggatggcg cagccgccag gctccgccgt ggccggcggt
      421 cgtgcaccag gctacgtcag ggaccggtcg cttcgcactg ccgcatcggt cagtgcgtga
      481 gaccaccgga gtagtcattg tccgtgctgt catagtcgta gtcgtcgaaa tccgcgagct
      541 ccatcttcag gctgcgatcc tccctcgaca tctcgatcag ccgccacaag ggcacgtgct
      601 cccgggggcc ttccgccacc tccttgaccg tgccctcctc ggcaaactcg tcaaactcaa
      661 gatcttcgtc ttccatatcc atagtcagtt cgatctccgg gtcttcttcg acccattccg
      721 gcaggggttc agtgtcgaca gatttggcca cgtttttatc cgccgggcca gtcgtttggt
      781 tccagaagat aaggctattt ggaatgatgt caagatttaa atcgggattg aatacaatga
      841 cttgacgcgt catttcgggt gtcgcgcggc cttcggcgga gggccctctc gaggggtcgc
      901 gtggaggacc ccgggaacgc ccggcttcct caggcagact ggcgggcgtc ctgctgggcc
      961 agttcgtgct cggcatcgcg ctcggacccg atcaggatgg ccagccagcg ggtgcggttg
     1021 tcgctctcca gcgcgagctt cagggtcatt gtcagcggca ccgacagcag catgccgacc
     1081 gggccgaata tccagcccca gaagatcagg ccggtgaaga cgatcagggg agagatgcct
     1141 acgccgtagc ccatccagcg cggctcgagg atgacgccga acatgacgtt gatcaccacg
     1201 aagccgatcg ccgtcgcagt cgcctcgccg atgccgagtt ggaccagggc cagcaggacc
     1261 gccggcaccg cggcgatgat cgagccgatc gtcggcacgt aattcagcag gaacgcgagc
     1321 atcccccaca gcaatgcgaa atccagcccg atgatcgagg tgacgagcca ggcggagaga
     1381 cccgtggcga tgctgatcag ggtcttgatg ccgaggtagc gccccaggtt gttcaaaaac
     1441 agcgtggcgt tttcgaacgt ctcggcgcgc cggccgaagg cagcaagaat cttggtttgc
     1501 acgctggagg cttccagcag gatgaatacc atcgtgaaca ggatcaggaa ggtgttggtc
     1561 aggacatccc gcagcgcgtt gagaatgttt gctaccagtc tcatagccca gccggcatcg
     1621 acgatgtcgc taaggccttc tatcgtgccg ccgttgtcca tgtgcgtggc ggcaaggcgc
     1681 aacaggcggt cgacgagcag gtccaggcgc gcctggtagg ccggcagagc tgcggtgaac
     1741 tcggcgatcg aagcaccgac aatggtgccc gcaaccgcaa gcaccagcat gatgaggacc
     1801 aggacgagca atgccgcgag gatcgagggc acccggtgcc tctgcagcca cagcatcgga
     1861 cgcaccgtga tcagcgcgag aaataccgca agcaaaaacg gcaccagcaa caccttcgcc
     1921 gcctgcatcc cgtacacaat gacgacgatg gcggcaagcg tgatcgctgt gctggtgttc
     1981 ctgcctgtaa tagtatccaa gcgtgccctt gtggcgatgt aaagcgtccg gcgccggccc
     2041 ggatgatcgc acaaaagccg cgccgaattc cctcccgccg cgggagtggg cggcgcgaat
     2101 ggggacaccc atcaaacggg gaggcgcgaa tggggacacc catcaaacgg gtgcggaatg
     2161 gggacaccca tcaaatcttg acagggcgga atggggacac ccatcaaatt ccgacgggaa
     2221 tgggacggga atcgggacac ccactaaatc tgtgggcggc caggagtcgc cgggcgtact
     2281 tctgagtcgg tcggataccg aattcgcggc agcggcttcc aggcttgagc tggagagagc
     2341 ttccagcgcc atgcgggcgt cgtcccggct cccttcccaa tgatcgtgta gcggctccct
     2401 ccggctcctt cccacggacc ggtggatgtc ccgcctctca atccgactcc ctgatcggaa
     2461 tgatggtggg tgtgccagtt ccgtccccgt ccgttccgac tcccgccgcg attccttgcc
     2521 cggagaatag tgggtgtccc gattccgttg tgggtgtccc gattccgttt ccgactcgct
     2581 gcgactccct ccccggagga attatgggtg tcccgactcc ggtgccgact ccggagtcag
     2641 gcggtagagg gctgggtttc cggttcgagg cgctttatgc tgaagccggt gacgccgtac
     2701 agaatcgata tcaggggatt gatcaggttg aggaaggcgt acggcgcgta actgaaggtg
     2761 gccactccca gcgtcgccgc catatatgcg ccgcaggtat tccacgggac cagggcggag
     2821 gtgagcgtcg ccgagtcttc cagggcacgt gacaggttct tggggtcgag gttgcgacgc
     2881 ttgaactcgg cctggaacat ctttccgggc agcacgatcg cgatgtattg gtcagcagcg
     2941 atgacattga tcccgatgca gctcagcacg acggtagtga tgagtgcacc cgtcgacttc
     3001 gcggctctga gggcgccctg gatgagtcgc gccagcatgc cggcatgctc catgaccgca
     3061 ccgaacgcca gtgccgcaag aatgagccag atcgtcgtca gcatgctgct catgccaccg
     3121 cgggtcagca gatcatcgac cgcgggcacg ccagtggatg ccacatagcc atcggcgagc
     3181 gccagccaca cccccttggt catcgcgagt ccggtcggca aaccgtctcc ctgcgaaaac
     3241 tccagcactg cctggggttg caatatcacc gccatgacac cgccgagcag tgcgccgaaa
     3301 agaatcgtcg gaagcggcgg cacttttcgc atcgcgaaaa cgaacaccac caccagaggg
     3361 atcaacgcga gcggcgtgac gttgaatgag ctgtccagcg tcgccatcag ccctcgcagc
     3421 tgaaccagat ccgaaccaac gtcgacgcca agtccgatga ccaggtacag gacgagcgcg
     3481 atcaggaacg aggggccggt cgtcagggac atatgccgga tgtgcgtgaa caggtctgtg
     3541 ccagcaaccg cgggcgcgag attcgtggtg tctgacaacg gcgacatctt gtcgccgaag
     3601 tatgaacccg aaatcacggc gccggctgct attgccggtg acagcgacag tcccattgcc
     3661 acgccgatca acgccacccc aagcgttccc gcggtcgtcc acgagcttcc tgttgcaagc
     3721 gacgagatcg cgcagataag gcaggtcgcg acaaagaaga agcgcggatc cagcagttcc
     3781 agcccgtagt aaatcagcgt cggcaccgtt cccgacatta accacgtccc gatcagcgcg
     3841 ccgacggcca gcaagatgag aatcgcgccc atagcggtac tgatgccgcc gatcatcgcg
     3901 ctttgcagtt cagcccactt gtgcccgttc ctgattgcga taatcgccgc gatcgcagca
     3961 cccattacca ggacgatttg cgtcgggcct gaagctgcat cggagccgaa caggtacaca
     4021 gaaagagcaa gcaagaggat cagcgagatg accgggatga tcgaatcgag cagagacggg
     4081 gacttgggtg ttgtcataga tctaccgtag tcggtttatg tttaactcgt gcaacgctcg
     4141 agtcgccgct gtttctctgc agcggtcgca gccggtaaat gtcccggcgg acgcaagcgt
     4201 accacggaaa cgcgtccctg ccttatgcaa tgctgccgat gctgcagcag ctgcataatc
     4261 tggcattcgc cgcattcttc cccgataatt cagtcatcga cgctcacgtc gatccttcta
     4321 aagttaccga gcgggtgatt tcatgcgtca gtccgtaata gcgatcgcga tacttcttgc
     4381 agtaaacctc gtcactctcg gattcgtgca atcggctggc gccggcgtta tcagcaccga
     4441 ggccgccatc gagatgcagg agcgtgaggc acgaatcgag cgcatcaatt ccg
//
LOCUS       DASUOM010000317         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_9710,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000317 DASUOM010000000
VERSION     DASUOM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9710"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFY27_06705"
     CDS             complement(<1..800)
                     /locus_tag="VFY27_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5765239.1"
                     /translation="MAQYIFTMNRVAKVVPPKRFILRDISLSFYPGAKIGVLGLNGSG
                     KSTLLRIMAGIDREFDGEARPQPGINIGYLPQEPALDATKNVRQNVEEGVAATKELLD
                     RFNDISMRFAEPMDDEQMSALLDEQGKLQDAIEAAGGWELDRMLEVAADALRLPPWDA
                     DVQTLSGGERRRVALCRLLISKPDMLLLDEPTNHLDAASVAWLERFLEEYPGTVIAVT
                     HDRYFLDNVAGWILELDRGHGIPWEGNYSSWLEQKEQRLATEEQTEKAR"
     gene            893..1861
                     /locus_tag="VFY27_06710"
     CDS             893..1861
                     /locus_tag="VFY27_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX5765240.1"
                     /translation="MRESRDSRPPVFVYRGRAIANYGFGNDHPFGPDRHDAFHAELAA
                     AALGSAVRYAEPRRASVDDLALFHTPEYIDRVSQLSQEGRGFLDAGDTPAFPGVFDAA
                     SDVVGTTLAAVDAVMRGETRRAFVPIAGLHHAARANAAGFCVFNDCGVAAEYLRKQYG
                     VERIAYVDIDAHHGDGMFYGFEDDPDLLFADIHEDGRFLYPGTGSADETGTGRARGTK
                     LNIPMAPGAGDGDFRTAWERVADYIDKASPDFIIFQCGADSLAGDPLTHLRYSEEAHA
                     AAARILCELADIHCEGRIVGTGGGGYDRRNLARAWTRVVQAFAARG"
     gene            1917..>2216
                     /gene="glyA"
                     /locus_tag="VFY27_06715"
     CDS             1917..>2216
                     /gene="glyA"
                     /locus_tag="VFY27_06715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777546.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5765241.1"
                     /translation="MYVETPTIEQFDSELFKAIVDEARRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAETLAIERAQKLFGAHYANVQPHSGSQ"
BASE COUNT          406 a          686 c          690 g          434 t
ORIGIN      
        1 cgcgcctttt ccgtctgttc ttcggttgcc aggcgctgtt ccttctgctc cagccaggaa
       61 gaatagttgc cctcccaggg tatgccgtga ccacggtcga gttcgagtat ccagccggca
      121 acgttgtcca ggaagtaacg gtcgtgggtc actgcgatga ccgtaccggg atattcttcg
      181 aggaaccgct cgagccacgc taccgatgcg gcatccaggt ggtttgtcgg ctcgtccagc
      241 agcaacatgt ccggctttga aatcagcaac cggcataacg ccacccgccg ccgttccccg
      301 cccgacagcg tctgcacatc ggcgtcccac ggcggcagtc tcagcgcatc ggccgcaact
      361 tcaagcatgc ggtccagttc ccagcctccg gcggcttcga tggcgtcctg cagcttgcct
      421 tgctcatcca gcaaggccga catctgctcg tcatccatgg gctcggcaaa tcgcatgctg
      481 atatcattga aacggtcgag gagttccttt gtggcggcga cgccttcctc gacgttctgg
      541 cggacgttct tcgtcgcatc cagcgccggt tcctgcggca ggtagccgat attgattccc
      601 ggctgcggcc gggcctcgcc gtcgaattcc ctgtcgatac cggccatgat tcgcagtaaa
      661 gtagattttc ccgacccgtt caggccaaga acgccgattt tggcgccagg atagaaactc
      721 aaggatatgt ccctgaggat aaatcgcttc ggcgggacca ccttggccac gcggttcatc
      781 gtgaagatat attgcgccat gcctgatctt ggttattccg gttgggtggg tttgataaca
      841 gaggcgcgca ttattgcata tcggttgtcc ggacctccgg agcacaggtg agatgagaga
      901 aagccgcgac agcaggcccc cggtgttcgt ctaccgcggc agggcgattg caaattacgg
      961 attcggcaac gaccatccgt tcgggcctga ccggcacgat gcctttcacg ccgagctggc
     1021 cgccgcggct ttagggtcgg ctgtgcgata tgccgaaccg cgccgcgcca gtgtcgacga
     1081 tctcgccctg ttccacacac cggagtacat cgaccgtgtg tcgcagttgt cacaggaagg
     1141 cagggggttt ctcgatgccg gcgacacccc ggcatttccc ggagtgttcg acgccgcctc
     1201 cgacgtcgtc ggcacgacgc tggccgcggt ggatgcggta atgcggggtg aaacacggcg
     1261 cgcattcgtg cccatcgccg gcctgcacca cgctgcgcga gcaaacgctg ccggcttctg
     1321 cgtgttcaat gactgcggcg tcgccgccga gtacttgcgt aaacagtacg gcgtggagcg
     1381 catcgcctac gtcgacatag atgcgcacca cggcgatggg atgttctatg gcttcgagga
     1441 tgatcccgac ctgctgttcg ccgacattca cgaggatggc cgtttcctgt atccgggcac
     1501 gggttccgcc gacgagacag gcacgggacg cgccaggggc acgaagttga acatcccgat
     1561 ggctccgggc gcgggcgacg gggacttccg gaccgcgtgg gaacgtgtcg ctgactatat
     1621 cgacaaggcg tccccggact tcatcatctt ccagtgcggc gccgacagcc ttgccggtga
     1681 tccgctgact catctccggt attccgaaga agctcacgcc gccgccgccc ggatcctgtg
     1741 cgaacttgca gatatacact gcgaggggcg catcgtcggg accggcggcg gcggctatga
     1801 ccggcgaaac ctcgcccggg catggacgcg cgtggtccag gcgttcgcag cgcgagggtg
     1861 agtgctgcgc tacactcacc gacttttctt aaccgacaga acggagatcg tgccgcatgt
     1921 atgttgagac gccgacgata gagcagttcg acagcgagct tttcaaggcc atcgtcgatg
     1981 aggcgcggcg gcaggaagag catatagagc tcatcgcctc cgaaaactac gtcagcccga
     2041 gggtgctgga ggcgcagggc tccgtgttga ccaacaagta cgcggaaggg tatccgggca
     2101 aacgctacta cggcggctgc gagttcgtcg acgtggcgga aacgctggcc atcgagcgcg
     2161 cgcagaagct gttcggcgcg cactatgcaa acgtgcagcc gcattcaggt tcgcag
//
LOCUS       DASUOM010000318         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1472852,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000318 DASUOM010000000
VERSION     DASUOM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472852"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="lepA"
                     /locus_tag="VFY27_06720"
     CDS             complement(<1..1189)
                     /gene="lepA"
                     /locus_tag="VFY27_06720"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5765242.1"
                     /translation="MNLIRNFSIIAHIDHGKSTLADRFIQLCGGLQDREMLEQVLDSM
                     DLERERGITIKAHSVSLKYPAPDGNVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQSVANCITAIDQGLEVLPVLNKIDLPAAEPDKVIHEIEDIIGIDAQQAAR
                     VSAKTGEGVAELLERIVKEIPSPRGDPDAPLQALIIDSWFDNYVGVVSLVRVMNGSLK
                     KGSKITIMSTQRSYNADRVGSFTPKMEDRGSLQTGEVGFVIAAIKDIYGAPVGDTITS
                     TARPCSAPLPGFKQIQPRVFAGLFPISSDDYENFRDALEKLRLNDAALHFEPETSAAL
                     GFGFRCGFLGMLHMEIVQERLEREYTLDLITTAPTVIFEVLMRDGKLLYVDNPSKLPP
                     VNEI"
     gene            complement(1306..1572)
                     /locus_tag="VFY27_06725"
     CDS             complement(1306..1572)
                     /locus_tag="VFY27_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5765243.1"
                     /translation="MRIFKVFSRQGCHLCELMVEELVPLVRGRALVEVVDVDTEPALR
                     EEYGLRVPVLACDGRMLCEYQLDRQAVEDALQGESGDEPARRRS"
     gene            complement(1585..2052)
                     /locus_tag="VFY27_06730"
     CDS             complement(1585..2052)
                     /locus_tag="VFY27_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HEX5765244.1"
                     /translation="MHQSRPDAPEGRVVATSGELVTVRVEAAVACARCAAGRGCGAGL
                     LQMGRTRIFEARVPRDLHLEPGDLVRLELEPDHLLQAAWVAYGLPLLGIILAVGAASV
                     IVDGGNESLVIACGLLGLLSGLMAGRRVLGKDECLHNMTPVAAHRVQAPTPHG"
     gene            complement(2073..>2466)
                     /locus_tag="VFY27_06735"
     CDS             complement(2073..>2466)
                     /locus_tag="VFY27_06735"
                     /inference="COORDINATES: protein motif:HMM:NF028497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX5765245.1"
                     /translation="NLESAVSQQALAPSIDLESFTWYTDPVRVDAAEVETDWVCDDLP
                     ADFRVISTRHEKLPGADSPVTHIVYGDGLATVSVFIAEQPDQEIARRSNLGAASSFSI
                     ESGEYQITAVGEVPAETVQRIASAMRMR"
BASE COUNT          474 a          780 c          743 g          469 t
ORIGIN      
        1 caatttcatt gaccggggga agcttggacg gattatccac atacaggagc ttcccgtccc
       61 gcatcaggac ctcgaatatg accgtcggcg cggtcgtaat gaggtccagc gtgtattccc
      121 tttccagccg ttcctggacg atttccatat gcagcatgcc taggaaaccg cagcggaagc
      181 cgaaaccgag cgctgcggag gtttccggtt cgaagtgcag ggcagcatcg ttcagccgca
      241 acttctccag ggcgtcgcga aagttttcat agtcgtcgga acttatgggg aacaggccgg
      301 caaatacgcg cggctggatc tgcttgaagc cgggcaaggg agcactgcag ggacgtgcgg
      361 tcgaggtgat ggtgtcgccg acgggcgctc cgtagatgtc cttgatcgcg gcgatgacga
      421 agccgacctc cccggtctgc aacgaaccgc gatcctccat tttcggtgtg aagctgccga
      481 cccggtcggc gttgtacgag cgctgggtcg acatgatcgt gatcttgctc cccttcttca
      541 ggctcccgtt cattacgcgc acgagtgaaa cgacgccgac gtaattgtca aaccacgaat
      601 cgatgatcaa cgcctgcagc ggtgcatccg gatccccgcg cggcgaagga atttccttga
      661 cgatacgctc caggagttcg gcgacgccct cgccggtctt ggcgctgacc cgcgccgcct
      721 gctgcgcatc gatgccgatg atgtcttcga tctcgtggat gaccttgtcg ggctctgccg
      781 ccggcaggtc gatcttgttc agtaccggga gaacctcgag gccctggtcg atcgcagtga
      841 tgcaattggc gacgctttgc gcttccacac cctgtgccgc gtcaacgacc agcagcgcgc
      901 cttcgcaggc cgcaagcgag cgcgatactt cgtacgagaa gtcgacgtgg cccggcgtgt
      961 cgatgaagtt gagctggtac acgtttccgt caggcgccgg atacttgagc gaaacgctat
     1021 gggccttgat ggtgatgcca cgctctctct ccaggtccat cgagtcgaga acctgctcga
     1081 gcatctcccg gtcctgcaac ccaccgcaaa gctgtatgaa gcggtccgcc agcgtcgact
     1141 tcccatggtc gatatgggca atgatcgaaa agttgcggat gaggttcatt gacaaaaggg
     1201 gcaatcctgc cgcgaacaag aggcgggctc cggcggcaaa cggcgaagta tatacgtttc
     1261 aggcagtccg tggccgggcg gctcggaccg acctgcagcg ggaagtcacg accgccgcct
     1321 tgccggctcg tcgccgcttt caccttgcag tgcatcctca acagcctggc ggtcgagctg
     1381 atactcgcaa agcatgcggc cgtcgcacgc caacaccggc acgcggaggc cgtactcttc
     1441 acgaagagcc ggttccgtat cgacgtccac gacctccacc agcgcacgtc cgcgcacgag
     1501 cggcacgagt tcttccacca tgagttcgca caggtggcag ccctgccggc tgaacacctt
     1561 gaaaatccgc attaccaggc cggcttatcc atgaggcgtc ggcgcctgaa cccggtgcgc
     1621 cgctacaggc gtcatgttat gcaggcattc gtccttcccc aggacacggc ggccggccat
     1681 gagcccggag aggagaccga gcaatccgca ggcaatcacc agcgattcgt tacccccgtc
     1741 aacaatgacg gaggcagcgc cgaccgcaag gatgatgccc agcagcggca aaccgtaagc
     1801 gacccatgcg gcctgcagca ggtggtccgg ctcgagctcg agccttacga ggtctcccgg
     1861 ttctaaatgc aggtcacgag gaaccctcgc ctcgaagatc cgggttcggc ccatttgcag
     1921 gagcccggcg ccacatccgc ggccggcagc acaccgcgcg cacgccacgg cagcttccac
     1981 tcgcacagta acgagttcgc cgctcgttgc tacgactctg ccttccggtg catctgggcg
     2041 agactggtgc aggggttgcc cggtgaggtc gtttagcgca tccgcatcgc actcgcgatg
     2101 cgttgcaccg tctcggcagg cacttcaccg acagcggtga tctgatattc gcccgactcg
     2161 atgctgaacg aacttgcggc gcccaggtta gagcgccgcg caatttcctg gtctggctgt
     2221 tcggcgatga acaccgacac ggttgccagg ccatctccgt acacgatgtg cgtgaccggg
     2281 ctatcggctc ccgggagttt ttcatgcctg gtggaaatca cgcggaagtc tgccggcagg
     2341 tcgtcgcata cccaatccgt ctcgacctcg gctgcatcga cgcggaccgg atccgtatac
     2401 caggtaaagc tttcgagatc tatcgacgga gcaagcgcct gttgcgacac tgcggattcg
     2461 agattg
//
LOCUS       DASUOM010000319         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_29280,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000319 DASUOM010000000
VERSION     DASUOM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29280"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFY27_06740"
     CDS             <1..781
                     /locus_tag="VFY27_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5765246.1"
                     /translation="LGMLGLHDVRENFGHWWLIDLLWACFCGLGVGGALGAGTAWLTR
                     RLNARGIYSEFTEDFLGLGLIALSYGLSLLLLGYGFLAVFAAGFMLHRSEMRYGMFSE
                     NREEVVRENHNDASSRGDESHMAKVTLGFVEQLERLGEAALLILLGGMLFADSWHGAY
                     VAAAVILIFLIRPLCVFVGLIGSEESFAIKATIAWFGVRGIGSLYYLMYAIQHGLETS
                     HAVTLISVVLIAVTLSIVLHGITVTPLMRLYAKRNEDSRRR"
     gene            complement(853..3045)
                     /locus_tag="VFY27_06745"
     CDS             complement(853..3045)
                     /locus_tag="VFY27_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5765247.1"
                     /translation="MSRVQSVERPGSHHKIHELIDQIGSGVPRAYLVRLAAQAPINGS
                     VTITVALLIAGFLLSEGYSSAVLAWAAVQVSLAALGLARWWKRPAEPHSLRDTRRSRR
                     GLYHALVWATISGSAWGALTAFLPGAPPHVQIVLILTMGGMAAGASATLAAVPQVAAM
                     YILACTVPATLYYVAVGGAAGGTLALVFGVFAAAMIAMSQVVFSALSRQFDAEWHAQE
                     LEEMAAALRESEQRFRATFQNAAVGIAHVGIDGSWLRVNDRVCEILGYSRDELITRTF
                     QDVTHPDDISAGLELLADMTRGESNGYQMEKRYLRKDGRLVWLHLTTALQRDDHGQPL
                     YGISVIQDITARKEAENALRESDQSKNEFLAVLGHELRNPLAPLRTGLEILEQAPRSL
                     ERLDNLLPMMNRQLSHLVRLVDDLLDISRISRGAIELQRAPLCLKAPLQAAVEQTTAL
                     VAQRRHHLVVQEPDEHVRVDGDFERLTQVFANLLDNAAKYMAPGGTIHVMSEEESGQA
                     VVRVRDEGYGIPHDRIDSLFKLFSQIPEHRDGAGGGLGIGLALVRRIIELHGGAIEAM
                     SEGLGCGSEFIVRLPTCSVEVEAHRADRRDASAGPALRVLVMDDNIDAAEGLRMLLEM
                     KGHSVKTAYDGPTALEQLEAFTPDVALLDLGLPGMDGIEVARRAQALPCGKGILLVAV
                     TGRGQDEDRHRTGRAGFDVHLTKPIEWHELDLILRARAGSQMSPVVCS"
     gene            3338..4213
                     /locus_tag="VFY27_06750"
     CDS             3338..4213
                     /locus_tag="VFY27_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765248.1"
                     /translation="MRLTIEFGFTPTRRLRRPLLPFAVLAATTASAMTWLEVDKLLPS
                     DGHEHAFFGHTVAIDDNTAIVATPFDAGAGEVGDFRGAAYVFARDNLGNWDAQAKLLP
                     AFSGFAHQFGYSVDIDGSTVIAGMFEEDLNPTEDAAYIFVRDASGNWREEAALTASDG
                     IFGDGFSVSVAVDGDTAFVGTRPETLTGFVDLTGAVYVFNRDASGTWSEQAKLVASDS
                     AIGDTFGRTLDLDGDTAIISRARDDDDGTGTVIVSAYIFTRDALGTWSEHTKLSTSRA
                     DSLRGRIRASTSGCY"
     gene            4263..4967
                     /locus_tag="VFY27_06755"
     CDS             4263..4967
                     /locus_tag="VFY27_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEX5765249.1"
                     /translation="MVYVFASDPSGNWSEDAVLTSSDRVAGDGFGSSVALFGETVVVG
                     APSDAGLDRGGAAYVFTRDTSGQWAEQKKLMASDGESGDLFGGAAAISDDTVIVGARH
                     HDAKRTDSGAAYVFQLSDFAEVEIKIRPHQEPNNVNPSSRQLIAVAILTTDAFDAVQV
                     DPLSVEFGPGGAAEFHGRGHARDIDKDGDIDLMLHFRMQQTGIACGDSQARLLGKTFA
                     GESITGSDSVRPKPCR"
     gene            complement(5092..5454)
                     /locus_tag="VFY27_06760"
     CDS             complement(5092..5454)
                     /locus_tag="VFY27_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765250.1"
                     /translation="MIEALEKVIGSGKGLGVEAYGSHQQSHRVTNRLVVVNDKNCLFL
                     GRRTQATSPVIGINFVRFDRGIVPVWIDNKQVAWQFLERPLGSCTDEEPLQAVSGDCA
                     HNDDGRAEHDVVNLIQRR"
     gene            5386..5916
                     /locus_tag="VFY27_06765"
     CDS             5386..5916
                     /locus_tag="VFY27_06765"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765251.1"
                     /translation="MRSVGLNSEAFATANDFLERFDQRSAGCLVLDIRMPGLSGLELQ
                     QRLLELGVSIPIIFITGHADVPMAVQAMQKGAFAFIQKPFRDQELLESISEAVISGNR
                     ERKKQETRAAVKARLQTLTRREQEVMDLVVAGKPNKVIAFELGVSQRTVEVHRARVME
                     KMQARSLASLIRMNLL"
BASE COUNT         1190 a         1783 c         1774 g         1262 t
ORIGIN      
        1 gctcggcatg ctgggcttgc acgatgtcag ggagaatttc ggccattggt ggctcatcga
       61 cctgttgtgg gcttgtttct gcgggctcgg tgtcggcggt gccctcgggg ccggcaccgc
      121 ctggctgaca cgccgtctca acgcacgagg catttacagc gagtttaccg aggactttct
      181 cgggctggga ttgatcgcgc tctcgtacgg gctgagcctc cttcttcttg gttatgggtt
      241 tctggccgtg ttcgcggccg gattcatgtt gcaccgctcg gagatgcgat acgggatgtt
      301 ttcggagaat cgggaagagg tcgttcgcga aaatcacaac gatgcgagca gcagggggga
      361 cgaaagccac atggccaagg ttacgttggg gttcgtagag caactggagc ggctgggcga
      421 agctgctttg ctgattctcc tgggcggcat gctgtttgcc gattcctggc acggagccta
      481 cgtcgcggcg gccgttatac tcatcttcct gattcgtcca ctttgcgttt tcgtcgggct
      541 cataggcagc gaggaaagtt ttgcaatcaa agctaccatc gcgtggttcg gcgtccgcgg
      601 cattgggtcg ctgtactatc tcatgtacgc aattcaacac gggctcgaga cgtcgcatgc
      661 cgttacgctg atctccgttg tcctgattgc ggttaccttg tcgattgtac tgcacggcat
      721 aaccgttact ccgctcatga ggctgtacgc gaagcgaaac gaagactcga gacgccgttg
      781 agcggtcaac ggattgacat acggctgtcg aatctggatt tcgcgttggt gttgccgacg
      841 gtaatgcccg ggtcaggagc acaccacggg cgacatctgc gagccagcac gcgctcgtaa
      901 gatgagatcg agctcgtgcc actcgatcgg cttcgtcaga tgaacgtcga agcccgcccg
      961 ccccgtccga tgccggtctt catcctggcc ccgtcccgtt acggcaacca aaagtatccc
     1021 cttgccgcac ggcagcgctt gggcccgccg ggccacctcg atcccatcca ttcccggcag
     1081 ccccagatcg agcagcgcga cgtccggggt gaaagcctca agctgctcga gcgcggtcgg
     1141 tccgtcgtac gctgttttga cagagtgacc cttcatctcc agtagcatcc gcagtccctc
     1201 ggcagcgtcg atattgtcat ccatcaccag cacccgtaag gccggtccag cggacgcgtc
     1261 cctgcggtcg gcccggtggg cttcgacttc cacggagcac gtcggcaggc gcacgatgaa
     1321 ctcgctaccg catccgagcc cttcgctcat tgcttcgatc gcgccgccat gcaactcaat
     1381 gattcgacgg accagagcca gtccgattcc caggcctccg ccggcgccgt cccgatgctc
     1441 tggaatctgg ctgaacagct taaagaggct gtcgatgcgg tcgtgtggaa taccgtagcc
     1501 ttcgtccctg acccgtacca cggcctgccc actctcctcc tcgctcatta cgtggatggt
     1561 gccgcccggt gccatgtact tggccgcgtt gtccaggaga ttggcgaaaa cctgcgtcag
     1621 ccgctcgaag tcaccatcca cccgcacatg ctcatcgggc tcctggacca caaggtggtg
     1681 tcgccgttgc gccaccagcg ccgtcgtttg ctcgaccgcc gcctgaagcg gagctttcag
     1741 gcagagcggc gcgcgttgca gctcgatggc accccggctg atgcgcgaga tgtcgagcag
     1801 atcgtccacc agccgcacca ggtgtgagag ctgccggttc atcatcggca ggagattgtc
     1861 gaggcgctcc aggctccgtg gagcctgctc caggatttcc aacccagtgc gcagcggcgc
     1921 cagcggattc ctgagctcat gccccagaac cgccaggaac tcattcttcg actggtcaga
     1981 ctcgcgcagc gcgttctccg cctccttgcg cgccgtgatg tcctgaatca ccgagatccc
     2041 gtacaaaggt tgcccatgat cgtcacgctg cagcgcagtc gtcagatgaa gccacaccag
     2101 gcgtccatcc ttgcgcaggt agcgcttctc catttggtag ccattggact cgccacgcgt
     2161 catatcggcg aggagctcga gacctgccga gatatcgtcg ggatgagtga cgtcctggaa
     2221 ggttctggtg atcagctcgt cgcgactgta gccgaggatt tcacaaaccc gatcgttgac
     2281 acgcaaccag gaaccatcta tgccgacatg agcgataccg actgccgcgt tctgaaaggt
     2341 cgcccggaaa cgctgctcgc tctcacgtag agcagctgcc atctcttcga gctcctgcgc
     2401 atgccattcg gcatcgaact gtctcgagag ggccgagaag accacttgcg acatggctat
     2461 catcgcggcc gcgaacacac cgaatacgag cgcaagcgtt ccgcccgccg caccgcccac
     2521 cgccacgtag tagagagtgg ccgggaccgt gcaggccagg atgtacatgg cggcgacttg
     2581 tggaacggcg gccaacgtcg ccgaagcccc tgcggccatg ccgcccatgg tcagaatcag
     2641 gacaatctgc acatgaggtg gtgcacccgg aagaaaagcg gtcaaggcgc cccacgcgct
     2701 tcctgagatc gttgcccaga ccagcgcgtg gtacaggcca cgtcggctac gcctggtgtc
     2761 tcgcagagaa tgcggctcag ccgggcgctt ccaccagcgt gccagcccca gagcggccag
     2821 gcttacctgg acggcggccc aggccaatac ggcactcgag tagccttccg agagcaggaa
     2881 cccggcgatc agcaacgcaa cggtgatcgt cacgctcccg ttgatcggcg cctgggcggc
     2941 gagacggacc agatatgcgc gcggaacgcc gctgccgatc tggtcgatga gctcgtggat
     3001 cttgtggtgg gagccagggc gttccacgct ttgcactctg ctcacgcgcg atccctcgac
     3061 ggattgacgc cgtcgccctc tcctgctccg agcaaggaat acatctggag aactccgccc
     3121 ttcgaaggcc gctgtgctgc actctcctgt tcactgcctt atgcatcacg acggcgtgga
     3181 agtctagccc agagttacag caagggtcct gcgtctcccc tgactttgca acctgcagtc
     3241 gctatccggt cttttcgtgg tactccggcc atcccagggg cgtgaccgcg cggcaaatcc
     3301 gccaccatct cctatactcc gctcagggat aaatacgatg cgtcttacca tcgaattcgg
     3361 attcacacct acaaggcggc tacgtcgtcc cttgctgccg ttcgccgtgt tggcagcaac
     3421 gacggcttcg gcaatgacgt ggctcgaggt agacaaacta cttccgagtg acggtcatga
     3481 gcacgccttt ttcggccata cggtcgccat agatgacaac acagcgatcg ttgcaacgcc
     3541 ttttgacgcg ggcgctggag aggtggggga ctttcgtgga gcggcatacg tatttgcgcg
     3601 cgacaaccta ggcaactggg atgcgcaggc aaaactcttg cctgccttta gcggcttcgc
     3661 tcatcaattc ggctactccg tcgatattga cggtagcacg gtaatagcag ggatgtttga
     3721 agaggaccta aatccaaccg aggatgcagc ctatattttc gttcgtgatg cttcgggaaa
     3781 ctggcgcgaa gaagctgcac ttacagccag cgacggcatt tttggtgatg gctttagtgt
     3841 ctctgttgct gttgacggtg acacggcgtt cgttggtacc cgaccagaaa cgttaacggg
     3901 cttcgtcgat ctgactggag cggtatatgt ttttaaccgg gatgcttcag gaacctggag
     3961 cgaacaggcg aagctagtgg cgagcgattc ggcaattggt gacacttttg gtagaactct
     4021 cgatctcgat ggcgataccg cgatcattag cagggcccgg gatgacgatg acgggacagg
     4081 aaccgtaata gtctcggctt acatcttcac tcgtgacgcc ctgggcacgt ggagcgaaca
     4141 cacgaagctc tcgacaagtc gagcggacag tcttcgaggg agaattcggg cctccacgtc
     4201 cggttgctat tgacggcaat attgctgttc ttggtactgg gccgaatcaa gacggtcacg
     4261 gtatggttta cgtttttgca agcgaccctt ccggaaactg gagcgaagat gctgtgctta
     4321 caagcagcga ccgcgttgct ggcgatggct ttggctcgtc agttgcgcta ttcggtgaga
     4381 cggttgttgt tggcgcaccg agtgacgcgg gtctagatcg tggcggcgca gcgtacgttt
     4441 tcacgcgaga tacttcagga cagtgggccg aacaaaagaa gctgatggcg agcgacggcg
     4501 aaagtggcga cttgtttggc ggggccgccg ccattagcga cgacactgtg atcgtagggg
     4561 ctcgccacca cgatgctaag cggaccgaca gcggcgcagc ttatgtattc cagctatccg
     4621 acttcgcgga agtcgagatc aaaattagac ctcaccaaga accaaacaac gtcaatccct
     4681 cctcccggca gttgatagcc gttgctatcc tcacgaccga tgcctttgat gcggtccaag
     4741 ttgatccgct atcggttgag ttcggtccgg gcggtgcagc ggagttccac ggccgcggtc
     4801 acgcacgaga catcgataag gacggtgaca ttgacctcat gttgcacttc aggatgcagc
     4861 aaacgggcat cgcttgcggc gatagtcagg ccagactttt aggcaagacc ttcgctggcg
     4921 agtccattac tggttcggat tctgtccggc ctaagccttg taggtgaccg cttttagcag
     4981 gcacacggct tgttcctgag atggggcgag attgcagcca tccggcaccg cgaacctctc
     5041 tcgacttgat caaggaactt ctcaatccta gtcctcgatc cctgagggcc gctacctgcg
     5101 ctgaatcaaa tttactacat cgtgttcagc gcgtccgtcg tcgttatggg cgcaatcgcc
     5161 agaaaccgcc tgaagcggct cttcgtcggt gcaactgccg agcggacgct ccaggaattg
     5221 ccatgcgacc tgcttattgt caatccagac tggtactatg ccccgatcga aacggacgaa
     5281 gttgataccg atcaccggtg aagttgcttg agtacgccga ccgagaaaca gacagttttt
     5341 gtcgttgacg acgaccaagc gattcgtcac gcgatgactc tgttgatgcg atccgtaggc
     5401 ctcaactccg aggcctttgc cactgccaat gactttctcg agcgcttcga tcaacgtagt
     5461 gcagggtgcc tggtgctcga cattcgcatg cccgggctga gcggccttga gttgcagcaa
     5521 cgtctgctcg aactaggagt atccatcccg attattttca tcaccggcca cgccgacgtg
     5581 ccaatggcgg ttcaagccat gcagaagggt gcattcgctt tcatacaaaa gccgtttcgg
     5641 gatcaggagt tgctggagag tatctcagag gcagtgattt ccggcaatcg agagcgaaag
     5701 aagcaggaaa cacgtgccgc agtcaaagca cgcctgcaga cactaacccg gcgagagcag
     5761 gaggtaatgg accttgtagt ggcgggcaag ccgaataaag ttatcgcgtt cgaactcggc
     5821 gtcagtcaga ggaccgtgga agttcaccgc gcgagggtga tggaaaaaat gcaggcccgt
     5881 tcacttgcga gcctgattcg catgaatctt ttgtgacagc cacgcggctc aattggccgc
     5941 acgccgcacc gacttgccgg gccggctatg gtagcgacgc ggatcaggcg ctcaatacgt
     6001 atattccga
//
LOCUS       DASUOM010000320         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_466912,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000320 DASUOM010000000
VERSION     DASUOM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466912"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFY27_06770"
     CDS             <1..438
                     /locus_tag="VFY27_06770"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HEX5765252.1"
                     /translation="QTTVMPRHNNPLKLSDNIEATMKQLLIGREGEYLGPLDSVREVC
                     RDLKSHHDALAEGMSTAFLEIMDRFDPAELQEQFDRGINRKPLIEAFSKIKYWQLYCD
                     FYPSMTEPGAAGLPQIFGEEFLRAYEKRLADSKRVNRSDSEAA"
     gene            complement(515..>2359)
                     /gene="acs"
                     /locus_tag="VFY27_06775"
     CDS             complement(515..>2359)
                     /gene="acs"
                     /locus_tag="VFY27_06775"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5765253.1"
                     /translation="NEAFWAEQAGRIDWTRKFSKVKDVSWAREDLHIRWYYDGTLNAC
                     YNCVDRHLQTKGDDVAIIWESDDPSVDLKITYRDLHGRVCRFANVLKKLGARKGDRIT
                     IYMPMIPEAAVAMLACARIGAVHSVVFGGFSPDALAGRIRDCESNIVITADQGVRGGK
                     ASPLKANVDEAARRDDVTTLEKVLVVRHTGADIDWLEGRDAWLHEVEVEVDADCPCAE
                     MNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYVAMTHQQVFDYRPGEVYWCTADVGWV
                     TGHSYIVYGPLANGAITLMFEGVPNYPTPARFWEVCDKHRVNICYTAPTAIRALMRFG
                     DEPVKQTSRKSLRLLGTVGEPINPEAWEWYYRVVGEERCPVIDTWWQTETGGILISPL
                     PGATDLKPGSATKPLFGIQPALVDNDGNFVEGAGEGNLVLLDSWPGQMRTVYGDHQRF
                     IDTYFSTYEGCYLTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESALVAHRYV
                     AEAAVVGCPHEVKGQGIYAYVTLMEGIEQTDELLAELRQWVRKEIGPIATPDFIQWAP
                     GLPKTRSGKIMRRILRKVAENDYSNLGDTSTLAEPAVVQDLIDNRKNR"
BASE COUNT          418 a          768 c          711 g          462 t
ORIGIN      
        1 cagacgaccg tgatgccgcg tcacaacaac ccgctcaagc tctccgacaa catcgaggcg
       61 acgatgaagc agttgctgat cggccgcgaa ggggaatacc tgggtccact ggacagcgtc
      121 cgggaagtct gccgggatct caagtctcac cacgacgcgt tggcagaagg gatgagcacc
      181 gcgttcctcg agattatgga ccgctttgac cctgccgaac tgcaggaaca gttcgatcgc
      241 ggcatcaacc ggaagcctct gatagaggct ttcagcaaga tcaagtactg gcagctgtat
      301 tgcgacttct acccgtcgat gacggaaccg ggcgcggcgg gactgccgca gattttcggc
      361 gaggagttcc tgcgggcata cgagaagcgg ctggccgatt ccaagcgcgt caatcgaagc
      421 gacagcgaag cggcgtagac cggacttctc gccacggaac gagacgtcgc ccgcgggcgg
      481 gccatctggt ctggccgtgg cgcaagacga caggtcagcg gttcttgcgg ttgtctatca
      541 ggtcctgcac gacggcgggt tcggccagcg tcgacgtgtc gccgaggttg gaataatcgt
      601 tttccgccac cttgcggagg atgcggcgca tgatcttgcc cgagcgcgtt ttcggcaggc
      661 cgggcgccca ctggatgaaa tccggtgttg cgatcgggcc gatctccttg cgcacccact
      721 gcctcagctc cgcaagcagc tcgtccgtct gctcgatgcc ttccatcaaa gtgacgtacg
      781 catagatgcc ctgccctttc acctcgtgcg ggcagcctac gaccgcggcc tcggcaacgt
      841 acctgtgcgc aaccagcgcg ctctcgactt ccgcggtgcc catacgatgc ccggagacgt
      901 tcagcacgtc gtccacccgg ccggttatcc agtagtaacc atcctcgtca cgtcgcgcac
      961 cgtcgccggt caggtaacag ccttcgtagg tggagaaata ggtgtcgata aagcgctggt
     1021 ggtctccgta cacggtgcgc atctgtcccg gccagctgtc cagcagcaca aggttccctt
     1081 cgcctgcgcc ctcgacgaag ttgccgtcgt tgtcgaccag cgccggctgg ataccgaaca
     1141 gcggcttggt agccgacccg ggcttcagat ccgtggcgcc cggcagcggg ctgatcagga
     1201 tgccgccggt ctccgtctgc caccaggtgt cgatcaccgg gcagcgttcc tcgccaacga
     1261 cgcggtagta ccattcccat gcttcgggat taatcggctc accgactgtg ccgagcaggc
     1321 gcaggctctt ccgtgacgtc tgcttcaccg gctcgtcgcc gaagcgcatc agtgcgcgta
     1381 tcgctgtcgg cgcggtgtaa cagatgttta cccggtgctt gtcgcagacc tcccagaacc
     1441 tcgccggggt cggatagttc ggcacgccct cgaacatcag cgtgatcgcg ccgttcgcaa
     1501 gcggcccgta cacgatgtag ctgtgcccgg tgacccagcc gacgtctgcg gtacaccagt
     1561 agacctcgcc cggacggtag tcgaatacct gttgatgagt catcgcgaca tagacgagat
     1621 agccgccggt cgtatgcaac acacccttcg gcttgcctgt ggacccggag gtgtagagga
     1681 tgaacagcgg gtcctccgcg ttcatctccg cgcatggaca gtccgcatcc acctcgacct
     1741 cgacttcgtg cagccaggcg tcccttcctt ccagccaatc gatgtctgcg ccggtgtgcc
     1801 ggaccacgag taccttctcg agcgtcgtca cgtcatcgcg cctggccgct tcgtcgacgt
     1861 tcgccttgag cggactcgct ttccctccgc gcacgccctg gtcggccgtg atgacgatgt
     1921 tcgattcgca gtcccgtatg cggccggcaa gcgcgtccgg ggagaatccg ccgaacacga
     1981 ccgagtgcac cgcgccgatc ctcgcgcagg cgagcatcgc cactgcggct tccggaatca
     2041 tcggcatgta aatcgtgatg cgatcgccct tgcgcgcgcc aagttttttc agcacgttgg
     2101 caaacctgca cacgcgtccg tgcagatcgc ggtaggtgat cttcaggtcg accgagggat
     2161 cgtcggactc ccagatgatt gcgacgtcgt cacctttggt ctgcaggtgc cggtcgacac
     2221 agttgtagca ggcattcagg gtgccgtcgt aataccagcg gatatgcagg tcctcccggg
     2281 cccaggagac atcttttact ttcgagaact tcctggtcca gtcgatgcgc cccgcctgct
     2341 ctgcccagaa ggcttcgtt
//
LOCUS       DASUOM010000321         4863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_812112,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000321 DASUOM010000000
VERSION     DASUOM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812112"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFY27_06780"
     CDS             complement(<1..1035)
                     /locus_tag="VFY27_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HEX5765254.1"
                     /translation="MKQTTSTLSLAAFLAVAAHASTTDIATGTGAESPSTIQERYITR
                     LEPEANIDSVAVAPGHPALLFATAKATDVVKVFDASTGNELAELGGEGASPEHYLRPN
                     GVAVADDLLVVVERDNRRVNVRSIPDYRALATFGEKELRSPYGVWVQALGTGDYRVFV
                     TDAYEMPDESVPPNEELDERVKSYRLEVARNAGGEATAAKAHFERAFGETESPGALRV
                     VESIFGDPAHDRLLIAEEDESPVTGLVIKVYDLEGRFTGEQIGKGIFGAQAEGIALYA
                     CDDGSGYWLSTDQADARTVYHVFHRQSLQHAGAFMGATTANTDGVWLATDGVPGFAGG
                     AFFAVHDDQAV"
     gene            1160..1816
                     /locus_tag="VFY27_06785"
     CDS             1160..1816
                     /locus_tag="VFY27_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765255.1"
                     /translation="MFLLLVEDDLNLGNALSKLLNQHYRVHWVRNLEAARNHFASAEY
                     DAMLLDLGLPDGDGVEWLQTLRSAGSNIPVLIVSARDALDDRVGGLDTGADDYLVKPF
                     EPEELLARIRVLLRRKSGRAQPRLCAGNLAYEAESRQFYLDDQPITLPPKEHQILAVL
                     MQAGEKPVSRERLMQQLYGLGDGVDSNTLEVHIHGLRKSLGKERIETVRGFGYKLAGA
                     "
     gene            1813..3153
                     /locus_tag="VFY27_06790"
     CDS             1813..3153
                     /locus_tag="VFY27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5765256.1"
                     /translation="MTFRLSHLALLLAVTIAVTAVGGVVATYRVADREFRDILDEDLE
                     IQSEFLAEFLSERKRLPDRQDLQELLEDAFEPDEDEDTRWVSVYDTRTGLHVSNLPHD
                     LALESTADRLVERTFDGHTWRGYQSEEDDVVVQILRRQDLFLDVRKDILEDIITPALV
                     GGAVNLLLLAILISYFTWPLTRLVRQLEIRSADSLAPIELRTPAREILVLRDALNRMM
                     SGVDTVLKRERGFASDVAHELRTPLTTLKLELANPEPDTRAVREEVDRLSRLVQQLLT
                     LARLEQGQWRKQFEPVELVPLFGRVVERFRRKFAEAGMTLHSELAEAAVEGDATLLDI
                     LLQNLLNNILNHCPAGTTAAACLETVAHPASARERQHGGYLRLRVSDTGPGISSEMRR
                     QMSRGFKRLDSKSEGFGLGLAICRKIAEVHGASLQFLSREDGRPGLVVEVAFPR"
     gene            3251..3613
                     /locus_tag="VFY27_06795"
     CDS             3251..3613
                     /locus_tag="VFY27_06795"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HEX5765257.1"
                     /translation="MKHSLHLLAAGALSVSALSVAAHAGGQGGFSGPDNLRVATVAEA
                     LELADDTHVKIRGQIARSLGDEKYEFTDGSATIVVEIDDDDWGGLEATPDMQVEISGE
                     IDEERSGNELDADTIRQL"
     gene            3757..4824
                     /locus_tag="VFY27_06800"
     CDS             3757..4824
                     /locus_tag="VFY27_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX5765258.1"
                     /translation="MFDPKRTLELVKGALLDPEPTWRSYLPEAFDWKKTALLLTGPLI
                     VAAALISWLLGFFASGSSLFGFGRPTLGTVLWQIVMGAILAGIVALIWSALAGAFRGK
                     SGFALGLAATTLAFVPGYLGQALSGLPWIGPLLALGLTVYSLVLLWRIIPLYLEVPDT
                     SRAAHFVVSILACIVAGFLVSTVIGSMMFEAGVAPGTRSFSGDDDSGAVRGGLFGAAS
                     RQAELLALAEEDTYTPPPDGKLNERQVENFLRVMDRAGELRAEKDERLQEIAEKAEDE
                     EQMSMSDFSQMMGGMVDLAGLQSEEIEVVKTAGGNWAEHQWVRESLRTAWIQKDINEA
                     VAHNFRLYQEYEDELAEHIAR"
BASE COUNT          916 a         1453 c         1568 g          926 t
ORIGIN      
        1 aactgcctgg tcgtcatgca cagcgaagaa agcgccgcct gcaaagccgg ggacgccatc
       61 ggtagccagc caaaccccat cggtgttggc cgtcgtcgcg cccatgaacg cacctgcgtg
      121 ctgcagggac tgtcgatgga agacgtggta gacggtgcgc gcgtcggcct ggtcggtgga
      181 cagccagtag ccggacccgt cgtcgcaggc atagagcgct atgccctcgg cctgggcccc
      241 gaagattccc ttgccgattt gttcgccggt aaagcgccct tcgagatcgt aaaccttgat
      301 cacgagtccg gtgacggggc tctcgtcctc ctcggcgatc agcagccggt catgtgccgg
      361 gtcgccgaag atggattcga cgacccgcaa ggctccgggg ctttcggttt caccgaacgc
      421 cctctcgaaa tgcgcttttg ccgcggttgc ctcgcccccc gcgttccggg ccacctccag
      481 gcgatagctc tttacccttt catccagttc ttcgttcggt ggcacgcttt catccggcat
      541 ttcgtaggca tcggtgacga atacgcggta gtcgccggtg ccgagcgcct gtacccacac
      601 gccgtagggg ctccgcaatt ctttttcacc gaaggtggcg agggcgcggt aatcggggat
      661 cgagcgcacg tttacgcggc ggttgtcgcg ctcgacgacc accaggagat cgtcagccac
      721 ggctacgccg ttcggtctca ggtaatgttc gggagacgct ccttcgccgc cgagctcggc
      781 gagttcgttc ccggtggagg catcgaagac cttcaccacg tccgttgcct ttgccgtggc
      841 gaagagaaga gccggatggc ccggcgcgac cgcgaccgag tcgatattcg cctccggttc
      901 cagcctggtg atatagcgct cctgtatggt ggagggagat tctgcaccgg tgccggtagc
      961 gatgtcggtg gtggaggcgt gtgccgcgac ggcaaggaac gcggcgaggc tcaaggtgct
     1021 tgttgtttgt ttcattgcga tgctccgtta ttgtccagta tctgcagtct gccggcagaa
     1081 acttaaggac agatgaagcg gtgcttaagc ctggcttaag gccgcatcgt cataatccgg
     1141 aaaactacag ggtggaccga tgttcctgtt gctggtcgaa gacgatctga atctaggcaa
     1201 tgcgttatcg aagttgctca accagcacta ccgcgtgcac tgggtgcgaa atcttgaagc
     1261 tgcgagaaat cactttgcga gtgcggagta cgatgcgatg ttgctggacc tcggcctgcc
     1321 ggacggtgat ggcgtggagt ggctgcagac attgcgatcc gctggctcga acatccccgt
     1381 cctcatcgtg agtgcgcggg atgcgcttga cgaccgcgtc gggggactcg atacgggagc
     1441 cgatgattat ctcgttaagc ccttcgagcc ggaggaactg ctggcacgca tacgtgtcct
     1501 gctgcgccgc aagtccggcc gcgctcaacc gcggctgtgc gccggcaacc tggcgtacga
     1561 ggcggagtcg cgccagtttt acctggacga ccagccgatc accctgccgc ccaaggagca
     1621 ccagattctc gccgtgctga tgcaggcggg ggagaagccg gtcagccgcg agcgcctgat
     1681 gcagcaactg tatggccttg gtgacggcgt cgattccaac acgctggaag tgcacatcca
     1741 cggactgcgc aagtcgctcg gcaaagagcg catcgagacg gtgcgcggtt tcggttacaa
     1801 gctcgccggc gcatgacctt tcggctttca cacctggctt tgctgctggc ggtcacgatt
     1861 gcggtgaccg cggtgggagg ggtagtggcc acgtaccggg tcgccgaccg ggagttccgt
     1921 gacatcctgg acgaagacct cgagatacaa agcgaattcc tggccgagtt cctgtccgag
     1981 cgcaagcgct tgcccgacag gcaagacctg caggagttgc tcgaggatgc gttcgaaccc
     2041 gacgaggatg aagacacgcg ctgggtcagc gtctacgaca cccggaccgg tctgcacgtc
     2101 agtaacctgc cgcacgacct cgcactggag tcgaccgccg accgcctggt ggaacggacc
     2161 ttcgatggcc atacgtggcg cggctaccag tctgaggaag acgacgtcgt cgtacaaatc
     2221 ctgcgccggc aggacctgtt cctcgacgtg cgcaaggaca tcctggagga catcatcacg
     2281 ccggcgctgg taggcggggc ggtcaacctc ctgttgctgg cgatcctgat cagctatttc
     2341 acctggccgc tgacacggct cgtccgccag ctcgaaatac gcagcgccga ctcgctggca
     2401 cccatcgaac tccgcactcc ggccagggaa atcctggtgc ttcgcgatgc cctcaatcga
     2461 atgatgagcg gcgtggacac ggtactgaag cgtgagcgcg gtttcgcaag cgacgtggca
     2521 cacgagcttc gcacgcctct taccaccctg aagctcgaac tggcgaaccc ggaacccgac
     2581 acccgggctg tccgggagga agtcgatcgg ttgtcgcgac tcgtccagca gctgctgacc
     2641 ctggcgcgcc tcgagcaggg acagtggcgc aagcagttcg agccggttga gctggtgccg
     2701 cttttcgggc gcgtcgtcga gcgcttccgt cggaagttcg ccgaggcggg catgaccttg
     2761 cacagcgaac tggccgaagc cgctgtagaa ggcgatgcaa cgctgctgga tatcctgctg
     2821 cagaacctgc tgaacaatat tctcaaccat tgtcccgcgg gcactacggc tgcggcgtgc
     2881 ctggaaaccg ttgcgcaccc ggcgtcggcg agggaacggc agcacggtgg gtacttgcga
     2941 ctcagggtca gcgatacggg gccgggaatt tccagcgaga tgcgccggca gatgtcgcga
     3001 ggattcaagc gcctcgacag caagagcgag gggttcggac tcgggctcgc aatctgccgg
     3061 aagatcgcgg aagtacatgg cgcatcgctt cagttcctgt cacgcgagga cggtaggccc
     3121 gggctcgtcg tcgaggtcgc gtttccacgc tgagattacg ttctgccggc aagctcccgg
     3181 actggcttaa gcctccttta aggttgcccc tgtaatctct ttcctcacca aacccacctg
     3241 gagattcacg atgaaacatt ctttacattt actggctgcc ggtgcgcttt ccgtgagcgc
     3301 tctttctgtt gccgcacatg caggcgggca gggtggcttc agcggccccg ataacctgcg
     3361 agttgccacg gttgcggaag cgctggaact ggccgatgac actcacgtaa agatacgcgg
     3421 ccagatcgcc aggtccctcg gcgatgagaa atacgagttc accgacggct ccgccacgat
     3481 cgtcgtggag atcgacgacg atgactgggg cggccttgaa gcaacgccgg acatgcaggt
     3541 cgaaatcagc ggcgaaatcg acgaggagcg aagcggcaat gagctggacg cagatacgat
     3601 ccggcagctg taaaggagag cgggaaagct taaaaactcc gctggaaaac gccccggcgc
     3661 aggtgcaaga ataggatgtc tggcggcctc gcggccgcct tcctggaggc aagtgcagca
     3721 ttccttgccc aaataataac tacaagggag acgtccatgt tcgatcccaa acgcaccctg
     3781 gaactcgtga agggagccct gctggatccc gagccgacct ggcgaagcta cctgcccgag
     3841 gcgttcgact ggaaaaagac cgcgcttctc ctgaccggtc cgctgatcgt cgcggcggcg
     3901 ctcatctcct ggttgctggg atttttcgct agcggatcgt cgcttttcgg tttcggccgg
     3961 ccgacgctcg gcactgttct ttggcagatc gtgatgggcg cgatactcgc cggtatcgtg
     4021 gctttgatct ggagcgcgct cgccggcgcg ttccgcggaa agagcggctt cgccctgggt
     4081 cttgccgcaa ccacgctcgc gttcgttccc ggctacctgg gacaagcgtt gtccgggctg
     4141 ccgtggatcg ggccactgct ggcactggga ctcacggtct actcgctggt gctgctgtgg
     4201 agaatcatcc cgctttatct cgaggttccc gacacttcgc gcgcggcgca tttcgtggtg
     4261 tcgatactcg cctgcatcgt cgccggattc ctggtgtcga ccgttatcgg cagcatgatg
     4321 ttcgaggctg gcgttgcccc tggaacgaga agcttttccg gcgacgacga ctccggtgcg
     4381 gtccgcggcg gtttgttcgg tgccgcatca cgccaggcgg aactgctggc gctggcggag
     4441 gaagacacct acacgccgcc gcctgacgga aagctcaacg agcggcaggt agaaaacttc
     4501 ctgcgtgtca tggatcgtgc gggcgagttg cgcgcggaaa aagacgagcg tctccaggaa
     4561 atcgcggaga aggcggaaga cgaagaacag atgtccatga gcgacttcag ccagatgatg
     4621 ggcggaatgg tcgacctggc cggcctgcag agcgaggaaa tcgaagtcgt gaaaaccgcc
     4681 ggcggcaact gggcggaaca ccagtgggtg cgggagtcct tgcgcacggc ctggatacag
     4741 aaagacatca atgaggcggt cgcccacaac ttcaggcttt accaggagta cgaagacgaa
     4801 ctggccgagc acatcgcgcg gtaggtaccg gaaaatctgc tacccgcatt agcgggttgt
     4861 agg
//
LOCUS       DASUOM010000322         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_857191,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000322 DASUOM010000000
VERSION     DASUOM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857191"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFY27_06805"
     CDS             <1..1321
                     /locus_tag="VFY27_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5765259.1"
                     /translation="ALLPVRADGILVRVFAGRGTDIVLGGNLEAELAAAGEDADREAI
                     RADWQQRHEAVLQAGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSMEDNLM
                     RIFGDPNRTKSLLSRAGMREGEAIESGMLSRQIERAQRKVEAHNFDIRKNLLEYDDVA
                     NDQRKVVYHQRTELMAADDIRDSVAAIREEVVAGVVEQYIPPGSIEDQWDPEGLAHAL
                     ERDFGVHLDIPGWLKADTKLNDEGIRQRCIEELQKNYEEKEQAIGSRLLRQVEKEVML
                     KQLDHHWKEHLAAMDYLRQGIHLRGYAQKNPKQEYKREAFEMFGMLLDQVKHDTISIL
                     SRIRIQSEQDLLRMEAQRRAVQEMQFKHAEAAALAQQSAAQPSALRQQPVTEVAPFPG
                     QPLPEGAQRAEPETHTPFVRGERKVGRNEPCPCGSGKKFKQCHGRLA"
     gene            1344..1760
                     /gene="mutT"
                     /locus_tag="VFY27_06810"
     CDS             1344..1760
                     /gene="mutT"
                     /locus_tag="VFY27_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEX5765260.1"
                     /translation="MRIRVAAGILRDGEGRVLLAERINDMSFRGLWEFPGGKIDPSEA
                     PEAGLCRELTEELGIVVTKLERLASVEHDYPDRLVHIDFFLVTEWRDEVRAMDGQALR
                     WVLPENINHDEILPADVEVIEALSRRSLSQPRNRPL"
     gene            complement(1812..2615)
                     /gene="zapD"
                     /locus_tag="VFY27_06815"
     CDS             complement(1812..2615)
                     /gene="zapD"
                     /locus_tag="VFY27_06815"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX5765261.1"
                     /translation="MARAASNIPTPVAGKRLVDYEQPLSERMRTFMRLEFLYQQLLYN
                     SELEADFATRAAVSGLLDIIAILTRGDVRSDAYKELDRQIESLQRYKSEPAVDTRRLD
                     SLIRNLAESRHAIDALGAQFLQPLKDNEFLSAIKHRSTIPGGACEFDLPVYSHWLRQP
                     YERRQKDLAEWMSTIRPLCDAVVELLWLTRETSQPVKKLAINGMYQYNVQKGANCRML
                     RVSLPDGSPLYPEISGSQHRFTIRFLEWSSVDRRAVQTGHDVAFQLSIC"
     gene            complement(2795..3415)
                     /gene="coaE"
                     /locus_tag="VFY27_06820"
     CDS             complement(2795..3415)
                     /gene="coaE"
                     /locus_tag="VFY27_06820"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195245.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5765262.1"
                     /translation="MDEKDPLRIGLTGGIASGKSMIADMFAARGVPIIDTDVIAREVV
                     QPGEPALNEIERQFGPEVISPDGRLDRRRLREIVFSDDGKREELEAILHPRIRDAAVL
                     QSSAAGGEYQVIVVPLLVESPMRQWMDRILVVDCSEETQLQRLLERDAESREQARRMI
                     RAQASREERLAIADDVIGNDRTKAEADDQVENLHRFYRGLANAAHG"
     gene            complement(3415..4305)
                     /locus_tag="VFY27_06825"
     CDS             complement(3415..4305)
                     /locus_tag="VFY27_06825"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5765263.1"
                     /translation="MHELFATSPLLFVLLIFFLALLIGSFLNVVIYRLPIMMEREWRA
                     QCLELVGSAASGKEASGERFDLLTPRSRCPSCGKPISALQNIPVVSYLFLRGRCASCK
                     TSISARYPIVELMTAVLAAIVAWRFGFGIEAAAAVLLTFALIAISVIDIDHQIIPDSI
                     SLPLVWIGLTFSLFHPMVGAEVLFVDPKTAIVGALAGYLSLWSVYHLFRLLTGKEGMG
                     YGDFKLLAALGAWLGWQMLPLIILLSAIVGACVGIAMIVFRRHDRNIPIPFGPYLAAA
                     GWVTMLYGSEIVDWYLGYMG"
     gene            complement(4545..>5123)
                     /locus_tag="VFY27_06830"
     CDS             complement(4545..>5123)
                     /locus_tag="VFY27_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018868663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5765264.1"
                     /translation="IIDLSVFVREKGWLLAILAGAGVSTFFYFKKRSRPMRHFLDRMA
                     LKTPIIGPILQKAAIARYARTLSTMFSAGVPLVEALESVSGATGNIVYEIGVLQMKDE
                     VSTGQRLQQAMENVNLFPNMVIQMVAVGEESGSLDEMSAKVADFYEEDVDNAVDNLSS
                     LLEPMIMAILGVLVGGLVVAMYLPIFKMGSVV"
BASE COUNT         1090 a         1580 c         1576 g          877 t
ORIGIN      
        1 ggcgctgctg ccagtccgcg cggatggcat ccttgtccgc gtcttcgccg gccgcggaac
       61 ggacatcgtg ctgggcggaa acctcgaagc agaacttgcc gccgccggcg aggatgcaga
      121 cagggaggca attcgcgcgg attggcagca gcgccacgag gcggtgctgc aggcaggcgg
      181 cctgcacatc atcggcaccg agcgtcacga atcccggcgc atcgacaacc agctgcgtgg
      241 ccgcgccggt cgccagggcg acccggggtc gagccgcttc tacctgtcga tggaagacaa
      301 cctgatgcgc atcttcggcg accccaaccg cacgaagtcg ctgctgtcac gtgccggtat
      361 gcgcgagggc gaggccatcg aaagcggcat gctgtcgcgg cagatcgaac gcgcgcaacg
      421 caaggtagaa gcgcacaact tcgacatccg caaaaacctc ctcgagtacg acgacgtggc
      481 gaacgaccag cgcaaggtgg tctaccacca gcgcaccgag ctgatggccg cagacgacat
      541 cagggattcc gtcgcggcaa tccgtgaaga ggtcgttgcc ggcgtggtcg agcagtacat
      601 tccgccaggc agcatcgagg accagtggga tccggaaggg ctggcgcacg cactcgagcg
      661 cgacttcggg gttcacctcg acatccccgg ctggctgaag gcggatacga agctgaacga
      721 tgaaggtatt cgccagcgtt gcatcgagga gttgcagaag aactacgaag agaaggagca
      781 ggccatcggc tctcgccttc tgcgccaggt cgaaaaagaa gtgatgctga agcagctcga
      841 ccaccactgg aaagaacacc tggcggcgat ggattacctg cggcagggta ttcacctgcg
      901 gggctacgcg cagaaaaacc cgaagcagga atacaagcgc gaggcgttcg aaatgttcgg
      961 catgcttctc gaccaggtca agcacgacac gatcagcatc ctttcgagaa tccgcatcca
     1021 gagcgagcag gacttgctgc gcatggaggc gcaacgccgg gccgtgcagg agatgcagtt
     1081 taagcacgcc gaagcggccg cgctcgccca acagtcggcg gcgcagccgt ctgcactacg
     1141 acaacagccg gtgacggagg tcgcaccgtt tcccggccag ccgttacccg aaggcgcaca
     1201 gcgcgcggag ccggagacac acacgccgtt tgtccgcggg gaacggaagg tgggacgaaa
     1261 cgaaccttgc ccgtgcgggt cgggcaagaa gttcaaacag tgtcacggaa ggctggcgta
     1321 acagcgacct cacaaagcgt caaatgcgca tccgggtagc agcgggcatc ctgcgggacg
     1381 gcgaaggccg ggtgctcctc gcggagcgga taaatgacat gagcttcagg gggttatggg
     1441 aattcccggg cggcaagatc gacccttccg aagcgcccga agccggcctt tgccgcgaac
     1501 tgacggaaga actggggatc gtcgtaacaa agctcgaacg cctggcaagc gtcgagcacg
     1561 actatcccga ccgcctcgtg cacatcgatt tcttcctggt gacggagtgg cgcgatgagg
     1621 tccgcgcgat ggacggccag gcgctccgat gggtgctccc cgagaatata aaccacgacg
     1681 agatcctgcc cgccgacgtc gaagtcatcg aagcactcag tcgtcgcagc ctgtcgcaac
     1741 ctcggaatcg accgctgtag gagcccgcca cgcgggcgag tacgataacc gcaacgaatc
     1801 ctgaaagcgc ttcagcaaat actcaactga aacgccacgt catgtccggt ttgcaccgcc
     1861 cgccgatcca ccgacgacca ctcgagaaac cggatcgtga accgatgctg gctcccgctg
     1921 atctccggat agagcggact tccgtccggc aacgagaccc gcaacatccg gcagttcgca
     1981 cctttctgca cgttgtactg gtacatgccg ttgatcgcga gcttcttcac aggctgcgac
     2041 gtttcgcgcg tcagccagag caactcgacc acggcatcgc aaagcggccg gatggtactc
     2101 atccactcgg ccaggtcctt ctggcgcctc tcgtaaggct gccgcagcca atggctgtac
     2161 accggcaggt cgaactcaca ggcgccgccg gggatggtgc tgcggtgctt gatcgcgctc
     2221 aggaattcgt tgtccttcag cggctgcagg aactgcgccc cgagagcgtc gatcgcgtgc
     2281 cggctttccg ccagattgcg gatgaggctg tcgagacgcc gcgtatccac ggccggctcg
     2341 ctcttgtagc gctgcaggga ttcgatctgc cggtcgagct ccttgtaagc gtcgctccgg
     2401 acgtctccgc gcgtcaggat ggcaatgatg tccaggagcc cggacaccgc cgcgcgcgtg
     2461 gcaaagtcgg cttcgagttc gctgttatag aggagttgct ggtagagaaa ttctaggcgc
     2521 atgaaggtac gcatgcgctc gctcaatggt tgctcgtagt ccaccaggcg ttttccggcg
     2581 acgggtgtag ggatgttcga tgctgctctt gccatgccgg tattctgcgc cagccatccc
     2641 gtcagggcaa atgactgtca acagcgacaa accgggatgg cagagtggtt gcacaggcgc
     2701 ggatagaaac gatgtaggag cccgccatgc gggcgagttc gctaacgtct gtcgcactcg
     2761 cccgcatggc gggctcctac aaaatcatta accgtcagcc gtgtgcagcg ttagccagac
     2821 cgcggtaaaa ccgatgcaga ttctcaacct gatcgtcagc ctcggccttc gtacggtcgt
     2881 tcccgataac atcatccgca atcgccagcc gctcctcccg actcgcctgc gccctgatca
     2941 tccgccgcgc ctgttcccgg ctttcggcat cccgctcgag aagccgctgc aactgggtct
     3001 cctcgctgca atccaccacc aggatccggt ccatccactg ccgcatcggc gattccacca
     3061 gcaacggtac gacgatgacc tggtattcgc cgccggctgc actggactgc aggacggccg
     3121 cgtcccgtat gcgcggatgc aggattgcct ccagttcctc acgcttgcca tcgtcggaga
     3181 agacaatctc gcgcagccgg cgccggtcga gacggccgtc cggagagatc acctccggcc
     3241 cgaactgccg ctcgatttca ttaagggcgg gttcgccggg ctgcacgact tcgcgggcaa
     3301 tgacgtcggt gtcgatgatc ggcacgcccc tggcagcgaa catgtcggca atcatgcttt
     3361 tgccgctggc gatgccgccg gtgaggccga tgcgcaatgg atccttttcg tccatcaccc
     3421 catgtaaccg agataccagt cgacgatctc gctgccgtaa agcatcgtca cccagccagc
     3481 ggcggcgagg tagggtccga aggggatcgg gatgttccgg tcgtgccgcc ggaagacgat
     3541 catagcgatg ccgacgcacg cgccgacgat ggcggacagc aggatgatca ggggaagcat
     3601 ctgccagccc agccacgcac cgagcgcggc aagcagtttg aagtcaccgt agcccatgcc
     3661 ttccttgcct gtcagcaagc ggaacaggtg gtacacgctc cacaggctaa ggtagccggc
     3721 gagcgcgccc acgatcgcag tcttcggatc gacgaagagc acttctgcac cgaccatcgg
     3781 gtggaacagg ctgaaggtca ggccgatcca caccagcggc agtgagatcg aatccgggat
     3841 gatctggtgg tcgatgtcga tgacgctgat ggcgatcagt gcaaaggtca gcaagacggc
     3901 agcggccgct tcgatcccga agccgaatcg ccaagccacg atggcggcga gtaccgcagt
     3961 catcagctcg acgatcgggt agcgcgcgga gatggaagtt ttgcaactcg cgcagcggcc
     4021 gcggaggaac aggtagctga cgaccggaat attttgtagc gcactgatcg gcttgccgca
     4081 cgaagggcac cgggaacggg gagtcagcag gtcaaaccgc tcgccgcttg cctctttgcc
     4141 ggaggccgct gagccgacca gctcgagaca ctgcgcgcgc cactcgcgct ccatcataat
     4201 gggcaggcgg tagatgacga cgtttagaaa actgccgatc aggagagcca agaagaaaat
     4261 aaggaggacg aagagcagcg gcgaagtggc aaagagttcg tgcatcattt gtcaggaatt
     4321 cgccgctcgt tgagtttgcg gtgcgttgtt gaccgtcgga tcgtgtgatg taggagcccg
     4381 ccatgcgggc gagtgcgatt cggcgtcggc gcactcgccc gcagtcgccc tctgggagct
     4441 aataacggat gaagcttcac cttgagcgtg ggctacaaca accttctgtc ccgcatggat
     4501 agtccgctgt aggagcccgt cgcgcgggcg acctgcttac accctcagac aaccgagccc
     4561 atcttgaaga tgggcaaata catcgcaacc acgagaccgc cgaccagaac acccaggatc
     4621 gccatgatca tcggctccag caagctgctg aggttatcaa ccgcgttatc tacgtcttcc
     4681 tcgtagaagt cggcaacctt cgccgacatt tcgtcaagcg agcccgactc ttcaccaact
     4741 gcaaccatct ggataaccat gttcgggaac aggttgacat tctccatagc ctgctgcagg
     4801 cgctggccag tagacacttc atccttcatc tgcaaaacgc cgatttcata aacgatgttc
     4861 ccggtcgccc cggaaaccga ttccagagcc tcaaccagag gtacacccgc gctgaacatg
     4921 gttgacagtg ttcgcgcata gcgagcgata gcggctttct gcagaatcgg gccgatgatg
     4981 ggcgtcttca gggccatgcg atcgaggaaa tggcgcatag ggcgagagcg cttcttgaag
     5041 taaaagaacg tggatacgcc agctcccgct agtattgcca gcagccagcc tttctcgcgc
     5101 acgaacaccg ataagtcgat aat
//
LOCUS       DASUOM010000323         7608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_606776,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000323 DASUOM010000000
VERSION     DASUOM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606776"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1138
                     /locus_tag="VFY27_06835"
     CDS             20..1138
                     /locus_tag="VFY27_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5765265.1"
                     /translation="MDYNCGRLSISMHIRSIFRGFGLSAVLVLLAACEQDVATGTQQE
                     PGPVKVVTYVAQSQRLLDEVQALGTARANESIEIRPRISSIVTRVAFTEGQLVREGDL
                     LIELENSEIRAGLAVAEAALSESRSTYNRSESLASTQAISASSLEQLRATMQVNEAQV
                     AAARARLENTVIRAPFGGRVGLRRVSPGGFVDTSTVITTLDDTDIIKLDFSIPETFLT
                     VVSSGMNIAAQSLVYPDRTFTGTVDSVDTRLDPVARSVQVRAVLENPDGALKPGMFLT
                     VDLQRDRGDVLVAPEEAIVPEREEQFVFQVVDGKAVKRQVSLGRRVPGLVEVTGGISA
                     GDILITEGTHKIRDGSLVEVVKHHAAEGIGGSGSEAQP"
     gene            1152..4280
                     /locus_tag="VFY27_06840"
     CDS             1152..4280
                     /locus_tag="VFY27_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5765266.1"
                     /translation="MLISDVSVRRPVFAAVISLILVIIGLMSVRSLPTREYPDIERPI
                     VSISTSYRGAASEVVERRVTQIIEDRIAGISGIEKISSASYDERSTITLEFAVDRDID
                     AAANDVRDRVSRVLNNLPEEADPPEVSKQDSSSATSMWINLSSDSRTIMDLTDYAQRY
                     LVDALSIVDGVAFVRLSGNRQPAMRVWIDPKLLAARQLTVTDIEDALRRENVQIPSGR
                     LESSQREFTLRTNTGYNTEDDFRNLVLAKGADGYLIRLGEVAQVQMAPEDVRGYSRTD
                     GLPGMSLGIIPQAKANLLQVNQDVRERIEDIRTTLPDDINLDVNIDLSIFISESLKEV
                     AKALGVALVLVLVVIYGFIGSFRATLIPAVTIPISIIASFAVMAWMGYSINVLTLLGL
                     VLAIGLVVDDAIIVLENIVRRIEKGEPALLAAVNGSREIGFAVIATTLVLVAVFLPVS
                     FMPGNIGRLFAEFGISLAAAVVFSSLVALTLVPMLTSKLFATGLHRGIVARAVDRVFQ
                     ALSNAYSAILRRTIRHPWIVIVSALAVFAASLTLLGRLPVEYMPREDRGMLLTMITAP
                     DGASLEYTAKYVREVEEILMADREGVLRFGTRSGSFRSGSDVNTAMIFAPLMLWEERS
                     RSAAEIAGQWNEKLADLPGVSAYAFVPGAGAAGQSSRPLQVVIGGTDYEELAAWRDIV
                     IREAEKLPGLTNLESDYKERKPKIDVSIDRDRAADLGVSLANVGRTLETILGSRVVTT
                     FIERGEEYEVILQGSDERRRTPNDLDSIYVRSDRTGQLIPLSNLVQLTEAAGPVDLRR
                     FDRMRAITISASLADGYSLGEALDALEEIIRANLPAAAQINYDGESRDLKTSGSSLYF
                     TFLLALVIVYLVLAAQFESFKHPFIIMTTVPLAVAGALIGLTAFGSSINIFSQIGAVI
                     LIGLAAKNGILIVEFANQLRDRGMEFREAVMEASRIRLRPVLMTGLCTAFGAVPLLLA
                     TGAGAESRRSIGAVVFFGVTFAVFLTLLVVPAVYSLVARKSHSPEYISQVIEKLKAAA
                     PAETKPAPSEAR"
     gene            complement(4343..4729)
                     /gene="rplQ"
                     /locus_tag="VFY27_06845"
     CDS             complement(4343..4729)
                     /gene="rplQ"
                     /locus_tag="VFY27_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5765267.1"
                     /translation="MRHKKAGRRLGRNSSHRQAMFRNMAASLLRHETIKTTLPKAKEL
                     RRVVEPLITLAKEDGVAKRRLAFDRLRDKEVVGKLFTDLGPRFQARPGGYLRILKLGP
                     RPGDSAPMAIVQLVDGPADSGESATS"
     gene            complement(4806..5807)
                     /gene="rpoA"
                     /locus_tag="VFY27_06850"
     CDS             complement(4806..5807)
                     /gene="rpoA"
                     /locus_tag="VFY27_06850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5765268.1"
                     /translation="MQESVHEFLKPRVVKVSPVDELHAKVTIEPFERGYGHTLGNALR
                     RILLSSMPGAAIVEAEIEGVLHEYTSIEGVQEDVVDILLNLKQVAVRMHGRDTAELRI
                     SKKGPGPVTGADIQLDHDVEIMNPDIVIANLTKAGELNMVLKVERGRGYRPATQRPAF
                     EEQSGPIGRLQLDASFSPVNRVTYDVESARVEQRTDLDKLIIDIETNGTIDPEEAIRR
                     AGSILKDQLSVFVDLQGQDEGAGARAESQIDPILMRPVDELELTVRSANCLKAENIMY
                     IGDLVQRTEVELLRTPNLGKKSLTEIKEVLEQHGLGLGMRLDNWPPASLRPEHELAV"
     gene            complement(5826..6446)
                     /gene="rpsD"
                     /locus_tag="VFY27_06855"
     CDS             complement(5826..6446)
                     /gene="rpsD"
                     /locus_tag="VFY27_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5765269.1"
                     /translation="MARYLGPKCKLSRREGTDLFLKSGVRPLESKCKLEVPPGGAAQR
                     RPRLSDYGMQLREKQKLRRMYGVLERQFSNYYKKAAQLKGSTGENLLRLLEGRLDNVV
                     YRMGFAATRAEARQLVSHKGVEVNGQVVNIPSAQVNAGDSIGIREKARKQLRIQSALE
                     IASQVGLPDWVEVDAKKMAGVLKSLPDRQDILPDINENLVVELYSK"
     gene            complement(6483..6866)
                     /gene="rpsK"
                     /locus_tag="VFY27_06860"
     CDS             complement(6483..6866)
                     /gene="rpsK"
                     /locus_tag="VFY27_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5765270.1"
                     /translation="MAEPQKRKKVKKQVVDAIAHIHASFNNTIITITDRQGNALSWAT
                     AGGCGFRGSRKSTPFAAQVASEKAGRQALEFGVKNVDVRVRGPGPGRESAVRALNALG
                     FRIQNIEDVTPIPHNGCRPPKKRRV"
     gene            complement(6924..7280)
                     /gene="rpsM"
                     /locus_tag="VFY27_06865"
     CDS             complement(6924..7280)
                     /gene="rpsM"
                     /locus_tag="VFY27_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5765271.1"
                     /translation="MARIAGINIPLNKHVGIALTSIFGIGRSRARQICQVAGIAPETP
                     VKDLAEPEVANIRSAVAKFIVEGDLRRENAMNIKRLMDLGCYRGIRHRRGLPVRGQRT
                     RTNARTRKGPRRPIKR"
     gene            complement(7298..7411)
                     /gene="rpmJ"
                     /locus_tag="VFY27_06870"
     CDS             complement(7298..7411)
                     /gene="rpmJ"
                     /locus_tag="VFY27_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5765272.1"
                     /translation="MKVRASVKKMCRNCKIIRRNGVVRVICTDARHKQRQG"
     gene            complement(7442..>7608)
                     /gene="secY"
                     /locus_tag="VFY27_06875"
     CDS             complement(7442..>7608)
                     /gene="secY"
                     /locus_tag="VFY27_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994260.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5765273.1"
                     /translation="PSIPFSFGGTSLLILVVVTMDFMSQMQAHAMSHQYEGMMQKANL
                     RNYGRSGQLR"
BASE COUNT         1370 a         2381 c         2315 g         1542 t
ORIGIN      
        1 agaaggtgct atatgaaatg tggactacaa ctgcgggcgc ctttcgatca gcatgcacat
       61 ccgttcgata ttccgcgggt tcgggctcag tgccgttctc gttcttttag ctgcctgcga
      121 acaggatgtg gcgaccggca cacagcagga gcccggccct gtgaaggtgg tgacctacgt
      181 cgcacagtca cagcgtcttc tcgacgaagt gcaggcgctg ggcactgccc gcgccaacga
      241 atcgatcgag atacgtccgc gcatctcgag catcgtcacc cgcgtcgctt tcaccgaagg
      301 ccagctcgtg cgcgagggcg acctgctgat cgaactcgag aatagcgaga tccgcgccgg
      361 ccttgcagtc gccgaagccg cgttgagcga aagccgcagt acctacaaca ggagcgaatc
      421 gctggcgagc acgcaggcaa tctccgcatc cagcctcgag caattgcgcg ccaccatgca
      481 ggtcaacgaa gcgcaggttg cggccgccag ggcgcggctg gagaacacag tgatacgcgc
      541 acccttcggc gggcgcgtcg gcctgcgccg cgtcagcccc gggggcttcg tcgacacttc
      601 taccgtgatc acgacgctgg acgataccga catcatcaag ctggactttt cgattcccga
      661 gacgttcctg acggtggttt ccagcgggat gaacatcgcg gcccaaagtc tcgtgtaccc
      721 ggaccgcacc tttaccggga cggtggacag cgtcgatacg cggctcgatc cggttgcgcg
      781 gtcggtgcag gttcgtgccg tgctcgagaa tcctgacggc gcactgaagc ccggtatgtt
      841 cctcactgtc gacctgcaac gcgatcgcgg cgacgtactc gtggctcccg aggaagcaat
      901 cgttcccgaa cgcgaagagc agttcgtttt ccaggtggtg gacggtaagg ccgtcaaacg
      961 ccaggtaagc ctggggcgcc gtgtgcccgg cctggtcgaa gtaacgggcg gcatatcggc
     1021 gggagacatc ctcatcaccg aaggcaccca caagatccgc gacggctcgc tcgtcgaagt
     1081 cgtcaagcat catgccgccg agggcatcgg cggaagcgga agcgaagcac agccatagac
     1141 gggctaacat catgctgatc tcagatgtat ccgtccgccg cccggtattc gcggccgtca
     1201 tttccctgat cctggtcatc atcggcctga tgtcggtccg cagcctgccg actcgcgaat
     1261 acccggacat cgagcggcca atcgtatcga tcagcacgag ctatcgcggt gcggcttctg
     1321 aagtcgtcga gcggcgcgtg acacagatca tagaggaccg catcgcgggc atctccggca
     1381 tcgagaaaat ttcctcggca agttacgacg agcgctcgac gatcaccctg gagttcgccg
     1441 tcgatcggga catagacgcc gctgcaaacg acgttcgcga tcgcgtatcg agggtactca
     1501 acaacctgcc ggaagaggcg gacccaccgg aagtctcgaa gcaggacagc tcctccgcca
     1561 cgtcgatgtg gatcaacctg tcgagcgaca gccgcacgat catggacctg accgactacg
     1621 cgcagcgtta cctcgtcgac gcgctgtcga tcgtcgacgg cgtagccttc gtccgcttga
     1681 gcggcaaccg ccagcccgcc atgcgggtct ggatcgatcc gaaactgctg gcagcccgcc
     1741 agctgacggt gacggacatc gaagatgcgc tgcgccggga aaacgtccag ataccgtccg
     1801 ggcgcctcga atcgagccag cgcgagttca cgctgcgcac caacacgggc tacaacaccg
     1861 aggacgactt ccggaacctg gtgctggcga agggagccga cggctacctc attcgcctgg
     1921 gtgaagtggc gcaggtacag atggcgccgg aggacgtgcg cggctactcg cgcaccgacg
     1981 gactgcccgg catgagcctg ggaatcatcc cgcaggcaaa ggccaatctc ctgcaggtga
     2041 accaggacgt tcgcgaacgc atcgaggata tccggactac cctccccgac gacatcaacc
     2101 tggacgtgaa catcgacctt tcgattttca tcagcgaatc cttgaaggag gtcgcgaaag
     2161 ccctcggagt cgcgctggtg ctggtccttg tcgtgatcta cggcttcatc ggcagcttcc
     2221 gcgccacgct gattccggcc gttaccatcc cgatctccat tatcgcgtct ttcgcggtga
     2281 tggcgtggat ggggtactcg atcaacgtgc tcacgcttct cgggctggta ctcgcgatcg
     2341 gcctggtggt agacgacgcc atcatcgtgc tcgagaatat cgtccggcgc atcgagaagg
     2401 gcgagccggc cctgctcgca gcggtcaacg gcagccgcga gatcggcttc gcggtaatcg
     2461 caacgacgct ggtcctggtt gcggtattcc tgcccgtgtc cttcatgccc ggaaacatcg
     2521 gccggctgtt cgccgaattc ggcatatcgc tggctgcggc ggtggtgttc tcatccctgg
     2581 ttgccctgac actggtaccg atgctgacga gcaagctgtt cgccaccggg ctgcaccgcg
     2641 gcatcgtcgc gcgcgccgta gaccgtgtgt tccaggcgct gtcgaacgcg tattcggcaa
     2701 ttctgcggcg gacgatccgg catccgtgga ttgtcattgt gtccgcactc gcggtattcg
     2761 ccgcgagcct gacgctgctc ggccgcctgc cagtggaata catgccgcga gaggatcgcg
     2821 ggatgttact gacgatgatc acggcacccg acggcgccag cctcgaatac accgcaaagt
     2881 acgtgcggga ggtcgaagag atcctgatgg ccgaccggga gggggtcctg cgcttcggca
     2941 cgcgatccgg gagctttcgc tccggcagtg acgtcaatac cgcgatgatt ttcgcaccgc
     3001 tcatgctgtg ggaggaacgc agtcgcagtg ccgccgagat cgccgggcag tggaacgaga
     3061 agctcgcaga cctgccggga gtgtcagcgt acgcattcgt cccgggagcc ggcgctgccg
     3121 gccagagttc ccgcccattg caggttgtca tcggcggtac ggactacgag gaactggcgg
     3181 cttggcggga tatcgtcatc cgcgaggcag aaaagctgcc gggcctcacg aatctcgaat
     3241 ccgactacaa ggaacgaaag ccgaagatcg acgtgtcgat cgatcgtgat cgcgccgccg
     3301 acctcggtgt ctcgctggca aatgtcggcc ggactctcga aacgattctc ggatcccggg
     3361 tggtgaccac attcatcgag cgcggcgagg agtacgaggt gatcctgcag ggcagtgacg
     3421 agagacgccg cacgccaaac gacctggatt cgatatacgt acgatccgac cgcacggggc
     3481 aactcatccc attgtcgaac ctcgtgcaac tcacggaggc ggcaggtccc gtggatctgc
     3541 gccgcttcga ccggatgcgg gcgatcacga tcagcgcgag cctggccgac ggctattcac
     3601 tcggggaagc gctggacgct ctcgaagaaa tcatccgcgc caatctcccc gcagcggcac
     3661 agatcaacta cgacggcgaa tcgcgcgacc tgaaaacctc cggcagctcg ctctacttca
     3721 cgttcctgct ggccctggtg atcgtctacc tggtgctggc ggcgcagttc gaaagcttca
     3781 agcacccgtt cattatcatg acgaccgtgc cgctggcggt ggcaggcgca ctcatcgggc
     3841 tcactgcttt cggctcctcg atcaacatct tcagccagat cggcgcggtc atattgatcg
     3901 gtttggcggc gaagaacggc attctcatcg tggagttcgc gaaccagctg cgggaccgcg
     3961 gcatggagtt ccgggaagcg gtgatggagg cctcccgcat tcgcctgcgc ccggtgctga
     4021 tgaccggcct gtgcaccgcg ttcggcgcgg tgccgctgct gctcgcgacg ggcgccggtg
     4081 cggagtccag gcgctcgatc ggcgccgtcg tgttcttcgg cgtgaccttc gcagtcttcc
     4141 tgaccctcct cgtagtcccc gccgtgtact cgctggttgc ccggaagtcg cactcaccgg
     4201 aatacatcag ccaggtgatc gagaagctga aagccgcggc ccccgcagaa acgaaacccg
     4261 ccccaagcga ggcaaggtag gagcagtgcg cttgtaggag ctcgaacgtt gcgggcgacg
     4321 atcgttggcg cgggctgcgc cgtcaggagg ttgcggattc tccgctgtcg gcggggccgt
     4381 caaccagctg tacgatagcc ataggggccg aatcccccgg gcgtggcccg agcttcagga
     4441 tacgcaggta accgccgggc cgggcctgga accgtggccc gagatccgtg aacagcttgc
     4501 cgacgacttc cttgtcgcgg agccggtcga aagcgagcct gcgcttggcg acgccgtctt
     4561 ccttcgcgag ggtgatcagc ggctccacta cgcgccgcag ttccttcgct ttgggcaggg
     4621 ttgtcttgat cgtctcgtgg cgcaacagcg acgcggccat gttgcgaaac atggcttgtc
     4681 gatgggagct gttacggcca agtcgacggc cggctttctt gtgacgcatg gcttttgcct
     4741 ttcagtattg ctgttgctgt gtgtcgacga ccaggcgcag cagtcatgct gcgcccgtcg
     4801 cctgactata cagcgagttc gtgttccgga cgcaggcttg ccggcggcca gttgtcgaga
     4861 cgcatgccca atccaagtcc gtgctgttcc agcacttcct tgatctcggt cagcgacttc
     4921 ttgccgaggt tcggcgtgcg aagaagctcc acctcggtgc gctgcaccag gtcgccgatg
     4981 tacatgatgt tctcggcctt caggcagttc gccgaccgga cggtgagttc cagttcgtcg
     5041 accggccgca tcaggatcgg gtcgatctgg ctttcagccc gggcacccgc accctcgtcc
     5101 tgtccctgca ggtccacgaa gaccgaaagc tggtccttca ggatgctgcc ggcgcggcgg
     5161 atggcttctt ccgggtcgat cgtcccgttc gtctcgatat cgataatgag cttgtcgaga
     5221 tcggtacgct gttcgacgcg ggcagactcg acatcgtagg ttacccgatt gaccgggctg
     5281 aacgatgcgt cgagttgcag gcgaccgatg ggtcccgact gctcctcgaa cgccggtcgc
     5341 tgcgttgccg gccggtagcc gcggccgcgc tccactttca gcaccatgtt cagctcgccg
     5401 gccttggtca ggttggcgat gacgatgtcc ggattcatga tttcgacgtc atggtccagc
     5461 tggatgtcgg caccggttac gggacccgga cccttcttcg agatgcgaag ttccgcggta
     5521 tcgcgaccgt gcatgcgcac tgcaacctgc ttcaggttca gcaatatgtc gacgacatct
     5581 tcctggacac cctcgatact ggtgtactca tgcagaacgc cttcgatctc tgcttctacg
     5641 atagcggcgc ccggcatgga cgagagcagg atgcgccgca gcgcgttgcc cagggtatga
     5701 ccgtagccgc gttcgaacgg ctcgatcgtg actttcgcat gtaattcgtc aaccggcgag
     5761 accttgacga cccgcggctt cagaaattcg tgtacagatt cctgcatggg taaaaaccct
     5821 cctgactatt tcgagtacag ctcgacgact aggttctcgt tgatgtccgg cagtatgtcc
     5881 tggcggtccg gcaacgactt gagtacgcct gccatctttt tcgcatccac ttcgacccag
     5941 tcgggcagac cgacctggct tgcaatttcc agggcacttt gaatgcggag ttgtttgcgg
     6001 gccttctcgc gaataccgat ggaatcgccg gcattcacct gtgcggacgg gatattgacg
     6061 acctgcccgt tcacttcaac gcccttgtga cttacaagct ggcgcgcttc ggcgcgcgtt
     6121 gcggcaaagc ccattcgata gactacgtta tccaggcggc cttcaagaag ccgcagcagg
     6181 ttttcacccg tggagccttt caactgtgcc gccttcttgt agtagttgct gaactgtcgc
     6241 tcgagtacgc catacatgcg gcgcagtttc tgcttttcac gaagctgcat gccgtagtcg
     6301 gaaagacgcg ggcggcgctg tgcagctccg ccgggcggaa cctccagctt gcacttcgat
     6361 tcgagcgggc gaacgccgct cttcagaaac aggtccgtgc cttcgcggcg ggacagtttg
     6421 cattttggtc ctagatatct tgccatcgtg atctctttcg gttgatctct ttcaattgat
     6481 ctctaaacgc ggcgcttttt tggcggacgg cagccgttgt gcggtatcgg cgttacgtcc
     6541 tcgatgttct ggatgcgaaa gcccagcgcg ttgagtgcac gaacggcaga ctcgcgtccc
     6601 ggacccggcc cacggacgcg aacgtcgacg ttcttcaccc cgaactcgag cgcctggcgc
     6661 ccggcctttt ccgacgcgac ctgggctgcg aacggggtcg acttgcgcga accgcgaaat
     6721 ccacagccgc ccgccgtcgc ccacgaaaga gcattaccct gccgatcggt gatcgtgatg
     6781 atcgtattgt tgaaggacgc atgtatgtgg gcgattgcgt ccaccacctg cttctttacc
     6841 ttcttgcgct tttgcggttc agccatttcg tggtcctgta aattgttcgc gtctgtccgt
     6901 cccggtatcc tgtaccagcc aggctatctc ttgatcggcc ggcgcggccc cttacgggtg
     6961 cgcgcgttcg tgcgggtccg ttgcccgcgt actggcaacc cacgccggtg gcggattccc
     7021 cggtagcagc caaggtccat caagcgcttg atattcatcg cgttttcgcg gcgaaggtca
     7081 ccctccacga tgaatttcgc gacggcgctg cggatgttcg ccacttcggg ttccgccaga
     7141 tcctttaccg gcgtctcggg tgcgataccg gcaacctggc agatctgccg tgccctggag
     7201 cgacctatgc cgaagatcga cgtcagcgcg atgccgacgt gcttgttcag cggaatgttt
     7261 atgcctgcta tacgtgccac tttcagtacc tttcctttta accctgacgc tgcttatgac
     7321 gcgcgtcggt acagatcacc cgcaccacgc cgttgcggcg gatgatcttg cagttcctgc
     7381 acattttctt gaccgatgcc ctgactttca tgagctctac tccgaaaccg ccgaagattc
     7441 ttcagcgaag ctggccgctg cgcccgtaat tgcgcaggtt tgccttttgc atcatgccct
     7501 cgtactgatg gctcattgca tgagcttgca tctgcgacat gaaatccatt gttacgacaa
     7561 ccaggatcag caacgacgtg ccgccgaaac tgaacgggat gctcgggt
//
LOCUS       DASUOM010000324         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1665637,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000324 DASUOM010000000
VERSION     DASUOM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665637"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1005
                     /locus_tag="VFY27_06880"
     CDS             49..1005
                     /locus_tag="VFY27_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5765274.1"
                     /translation="MKAVFLDFSTLGPDELDTTPLLQVLPGIEFFDSTEPDLTVSRIR
                     DAEFVLTNKARLDEVTLASTTRLKFIGLTATGTDNVDLVAARRHGIAVANIRAYCTRS
                     VVEHVFGVLLGLTHSLNRYHRAVKEGDWRHSDVFCLLDYPIRELSAMTLGIVGYGELG
                     RGVAAMAKTFGMTTLIARRRGAPAGEDGRTGFNELLERSDIVSLHCPLTPDTRDLIGA
                     AELRRMKRNAFLINTARGGLVDSRALVDALREGRLAGAGIDVLREEPPVHGDPLLEYS
                     GANLILTPHIAWATVEARQNAIAELAMNVVAFLEGTSRNRVV"
     gene            complement(1030..1452)
                     /locus_tag="VFY27_06885"
     CDS             complement(1030..1452)
                     /locus_tag="VFY27_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5765275.1"
                     /translation="MSQLPDTLSCGIVIVRRTDGGWLTLMLRAYRNWDFPKGLMEQGE
                     TPIEAAVREVGEETGITQLSFDWGDKFIETGPYNRGKVARYYLARTEEEKVILGIVPA
                     LGRAEHHQSLWVDFDKAYDLTAPRVRGVVQWARQIVGA"
     gene            complement(1466..>2763)
                     /locus_tag="VFY27_06890"
     CDS             complement(1466..>2763)
                     /locus_tag="VFY27_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5765276.1"
                     /translation="SLAAVTLLLYQAIPDARMLAIVLGGMLVVAAALYLAGRLLVALM
                     GRSRGGVGIAWRYGLANVARRGRDSAVQVVAFGLGLTVLLLLSFVRTDLLEGWRQSLD
                     EDAPNHFLINIQPHERESIASIFRSEGIAEPQFVPLVRARMTEINGESVKTRTYPEED
                     GEWMANREANLTWASELSTSNTILEGEWWESSYDGEPLVSVEEEAAMEMGLEIGDRLR
                     FLVAGQEVEARVVSFREVNWDSFQPNFFLVLSPGALDDFPTTYVSSLQVEDDQQGALL
                     RLVRTHPSVSVIDLGAILEQVQSIISKASLAVQAVFVFTLAAGIAVLFAAVQSTIDER
                     RFESAMLRALGVRRRTVLSGVLTEFAALGFAAGLLAAIGASILAAVISDQLFDLGYSF
                     DPMLWIAGLGGGVMLVCLSGYIAARSAINAPPADVLRAA"
BASE COUNT          528 a          888 c          851 g          496 t
ORIGIN      
        1 aacggtatcg cgcagggact gcagaacacc ggctgaacta tcttcacgat gaaagccgtg
       61 tttctcgatt tttcgacgct cgggccggat gagctcgata ccacgccact gttgcaggtc
      121 ctgcccggca tcgaattctt cgactcgaca gagccggacc tcacggtctc aagaatccgt
      181 gatgcggagt tcgtactgac gaacaaggcc aggctggatg aagtgacgct cgcgtcgaca
      241 acgcggctga agttcatcgg actgacggca acggggacgg acaatgtcga tctcgttgcc
      301 gcacggaggc acggaatcgc agttgcgaac attcgtgctt attgcacgcg ctcggtggtc
      361 gaacacgtat tcggggtcct gcttggcctg acgcacagcc tgaaccgcta ccatcgtgcg
      421 gtgaaggagg gagactggcg gcactcggac gtattctgcc ttctcgatta ccctataagg
      481 gaactgtcgg cgatgacgct cggcatcgtc ggttacggtg aactgggtag aggcgtcgct
      541 gccatggcga agacgttcgg catgacgacg ctgatcgcga gacgccgcgg agcgcccgcg
      601 ggagaagacg gccgcaccgg cttcaatgaa cttctcgagc gttccgacat cgtgtcgctg
      661 cattgcccgc tgacgccgga tacccgggac ctgatcggag ctgccgagtt gcggcggatg
      721 aagcgcaatg cgttcctgat caataccgca cgcggcggtc tcgtcgactc ccgcgcgctg
      781 gtcgatgcac tacgcgaggg aagactggcc ggcgcgggaa tcgacgtcct gcgcgaggag
      841 ccgccggtgc atggcgaccc gttgctcgaa tattccggcg ccaatctcat cctgactccg
      901 cacatcgcct gggccacggt agaggctcgg cagaacgcca ttgccgaact tgcgatgaac
      961 gtcgtcgcct ttctcgaggg tacgtctcgg aaccgtgtgg tttgaggttt atatctgccg
     1021 tggaggctct taggcgccaa caatttgtct tgcccactgg accacgccac ggacgcgtgg
     1081 agcggtgagg tcgtaggctt tgtcgaagtc gacccacagg ctctggtggt gctcggcccg
     1141 gcccagggca gggacaatgc cgaggatcac cttttcctct tcggtccttg cgaggtagta
     1201 gcgagcgacc ttgccgcggt tgtaggggcc ggtttcgatg aacttgtcgc cccagtcgaa
     1261 ggacagctgg gtgatgccgg tctcttcgcc gacttccctg acagcagctt cgatcggcgt
     1321 ctcgccctgc tccatcaggc ctttcgggaa atcccagttg cggtaagcgc gcaacatcag
     1381 cgtgagccac ccgccatcgg tcctgcgaac gatgacgatg ccgcaggaga gggtgtcggg
     1441 aagctggctc atgtcctgga atgcgtcagg cggcgcgcag cacgtcggcg ggcggcgcgt
     1501 tgatggcgct gcgggcggcg atatagccgc tcaggcagac gagcatgacg ccgcctccga
     1561 gcccggcgat ccacagcatc ggatcgaagc tgtagcctaa gtcgaacagc tggtcggaaa
     1621 tgacggctgc cagtatcgag gccccgatcg cagccagcag ccccgcggca aaacccagcg
     1681 ctgcaaactc cgtcaacacg cccgacaata ccgtgcgccg ccgcaccccg agcgcccgca
     1741 gcatcgcgct ctcgaaacgc cgctcgtcga tggttgactg cactgcggcg aaaagcactg
     1801 caattccggc tgcaagcgta aagacgaata cggcctgcac ggcaaggctc gctttcgaga
     1861 tgatgctctg cacctgttcc aggatggcgc cgagatcgat gacggacacg ctcggatgag
     1921 tcctcaccag gcgcagcagc gcgccctgct ggtcgtcctc gacctgcagg ctggacacgt
     1981 aagtcgtcgg gaagtcatcg agcgccccgg gcgacagcac gaggaagaaa ttcggctgga
     2041 atgaatccca gttcacttcg cggaagctta cgaccctcgc ttccacttcc tggccggcga
     2101 cgagaaagcg caaacggtcg ccgatctcga gacccatttc cattgcagcc tcttcctcaa
     2161 cagagaccag cggctcaccg tcgtatgaag actcccacca ttccccttcg agaatcgtgt
     2221 tgctggtgct cagctcgctc gcccaggtca ggttggcctc gcggttcgcc atccactccc
     2281 cgtcctcctc cgggtaagtg cgggtcttca ccgactcgcc gttgatctcg gtcatgcgcg
     2341 cgcgcaccaa cggcacgaat tgtggttcgg cgatgccctc gctccggaaa atactcgcaa
     2401 tcgattcgcg ctcatgcggc tggatgttga tcaggaagtg gttcggcgca tcctcgtcga
     2461 ggctttgccg ccacccttcc agcaggtccg tgcgcacgaa cgacaacagt agcagcaccg
     2521 tgagcccgag cccgaacgcc acgacctgca cggcgctgtc acgcccccgt ctcgcgacgt
     2581 tagcgagccc gtaccgccag gcgatcccga ccccgccgcg cgaacggccc atcaatgcaa
     2641 cgagcaggcg cccggccagg taaagcgccg ccgcaacgac cagcatcccg ccaagcacaa
     2701 tcgccagcat gcgcgcgtcg gggatcgcct ggtaaagaag cagcgtcact gcggcgagcg
     2761 atg
//
LOCUS       DASUOM010000325         5188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_648221,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000325 DASUOM010000000
VERSION     DASUOM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648221"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFY27_06895"
     CDS             complement(<1..508)
                     /locus_tag="VFY27_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765277.1"
                     /translation="MKYRTKYPFSAPKQSRHVRLTALAAALVAVASLQAAVAAPGTEN
                     GQWHYLGGDSAHTRYSPADQVDKESFGDLEEAWIWDGASFNAQSGRSTPSYINGRLYT
                     VAGPRRHVVAIDPETGETLWSYREPNTARWEYSMRQDYGKGVAYAEIDGRGVIYIISP
                     AFFLTALDA"
     gene            890..2110
                     /locus_tag="VFY27_06900"
     CDS             890..2110
                     /locus_tag="VFY27_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5765278.1"
                     /translation="MKKRVLVLGAGVTGVVTAWYLSQKGHDVTVVETSDGVASGTSRA
                     NAGQLSYSFTDALARPEFLRKVPGLMMGQDAGILVRAKPDAPFLRWGRQFLRQCTTVR
                     ASDNTVAVLRLALRSAALMEQLMQAVPLEFDFARAGKLVLWSRPDEVARARKSVLLKR
                     EYGCLTEVLTRGEALEVEPALDNMRGDFVAATYSRNDHVADARKFTLGLADWLKREAS
                     VDFRLRETAARLVVEGNRVSKVLTERNEYNADAVVVCLGTGSTKLLEPHGIDPAICPV
                     RGYSITLPLGPHAPSASISDPGRRIVLSRLGSSIRIAGFADFVGYETRHDEARLDALV
                     AMARRVAPLAADYDVGDMQRWGGLRPMTPDGRPRVGPTRVGGLFLNTGHGMLGFTLAL
                     ATGEAVSEQVSARS"
     gene            complement(2137..3471)
                     /locus_tag="VFY27_06905"
     CDS             complement(2137..3471)
                     /locus_tag="VFY27_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765279.1"
                     /translation="MLAAATSVAASLAVAGCGEERRMESAPDLDDLARNYVVLELAMG
                     LHDGNHVDAWYGPEDLRQAAEDAALTLPQIADEAEALANRVATWPGNRSNRMQLKRID
                     GLQQRLRALSTRVAINQGRDFSFDEESRLLFGVTAPDYDATHFEEILERIGALLPGDG
                     SLSARVNDFQNRFVIPSDRLASVFDAAIAECRRRTLERINLPENESFSLEYVTDKPWS
                     GYNWYQGRSQSLIQINTDLPIFISRAIDLGCHEGYPGHHTFNVLLEKNLVEREGWVEF
                     TLYPLFSPASLIAEGSGNYGVELAFPDDERVQFEKAELFPLAGLDPAEADRYYALQDL
                     LDELSYAGNEAARDYLNGNITREQAAEWLEEYALSSPERARQRVDFFDTYRSYVINYN
                     LGQDLVRDYVERDTGTDGNGQGSAIELRWRRFAEMLSTPTSPSDLAGTADDG"
     gene            3702..4514
                     /locus_tag="VFY27_06910"
     CDS             3702..4514
                     /locus_tag="VFY27_06910"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX5765280.1"
                     /translation="MRKDVLQWLIPVLLAILLAAALVFYWRQSNRPAGDIEPNLRTDP
                     PETTQEAPPAAPEHPLETTGEAAAEQPELRPLPPLEESDEYFSLEISELFGPALSEKL
                     IEQNLVERVVATIDNLPREQIADRIKPMGGVGGQLVVNEGEEEGEYVLAPENYARYNA
                     LVGMVANSDIAEVEELYRRFFPLFQNAYVNLGYPDGYFNDRVIEVIDHLLDTPEPEGE
                     LILTRPHVLYEFADPELEDLSPGQKLLLRMGGEHRATVKEKLREFRARIVSE"
     gene            4563..>5188
                     /locus_tag="VFY27_06915"
     CDS             4563..>5188
                     /locus_tag="VFY27_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX5765281.1"
                     /translation="MPATLAIAAVNHVGTGSVPPMAGFDEQAAQAQRTLEKAFDEDLS
                     AADQDEWLRDLSARPHHVGSRAGRENARYIADLFESWGYDVEIEEYGILLPTPETREL
                     ELLAPYAFTASLEEDALPQDPSTSVRGELLPPYNAFSTDGEVEGELVFVNYGIPEDYE
                     LLERHGIDVRGKIAIAKYGRSWRGIKPKLAGEKGAIGTIIYSDPADDG"
BASE COUNT          979 a         1589 c         1615 g         1005 t
ORIGIN      
        1 ccgcgtccag tgccgtcagg aagaatgccg gcgagatgat gtagatgacg ccacgcccgt
       61 cgatttccgc gtaagcgacg cccttgccgt agtcctggcg catcgagtac tcccagcgag
      121 cggtattggg ctcgcggtac gaccagagcg tctcgccggt ttccggatcg atggcaacga
      181 cgtgtcggcg gggtcccgcg accgtataca acctgccgtt gatatagctc ggcgtggacc
      241 ggccgctctg tgcattgaag ctcgcgccgt cccagatcca ggcttcctcg aggtcgccaa
      301 agctttcctt gtcgacctgg tcggccggcg aatatcgtgt gtgagccgaa tcgccgccga
      361 gataatgcca ctggccgttc tcggtgccgg gagccgcaac ggccgcctgg aggctcgcaa
      421 cagcgaccaa cgcggccgcc aacgccgtca agcgaacgtg ccgagattgt ttcggcgctg
      481 aaaatggata cttcgttcgg tacttcatgt tatctccgat gggctccgtt ttttccggtt
      541 gagcgcgaac acccgggact gctttgcatt aaacgccgat tccggcgcgc ggcgtgcgtt
      601 tcacgatatg accaggacgg catacttgat ttgtcccctc ctttcgagcc atctcgttat
      661 ttgacaagcg ctttaacctg ttcttcgaca gtgaaccgcg aacttgtcat tattcgttcc
      721 cgaaagtgca gggcggaaga atacctgagg ccccacggca gtaaaactgt ttcaccacaa
      781 tgcaaagcgc tggcaggcgc cagccggagt agccgatgtt cccgggccga tcccgcagtt
      841 ttcatccccg acagcagcct tccctgtttt cgcaaatccc agccgccagg tgaaaaagcg
      901 cgtactcgtt cttggcgccg gggtgaccgg cgttgtgacg gcatggtacc tgtcgcagaa
      961 gggtcatgac gtcactgtag tggaaaccag cgatggcgtc gcatccggca cgagccgcgc
     1021 gaatgcgggc cagctgtcgt acagctttac cgatgcgctg gcgcggccgg aattcctgcg
     1081 caaggtgccg gggctcatga tggggcagga tgccggcata cttgtccggg cgaagccgga
     1141 cgcgcccttc ctgcggtggg gccggcagtt cctgcgccag tgcacgaccg tgcgagcatc
     1201 cgacaacacg gtcgcggtgc taaggctcgc cctgcgttcg gccgccctga tggagcaatt
     1261 gatgcaggcc gtgccgctcg agttcgattt tgcgcgcgcc ggtaagctgg tcctgtggtc
     1321 tcgcccggac gaggtggcgc gcgcgcgaaa atcggtcttg ctcaagcggg agtacggctg
     1381 cctgaccgag gtactgacgc gcggcgaggc gctggaagtc gagcccgcac tcgacaacat
     1441 gcgcggtgat tttgtcgccg cgacctactc gcgtaacgat catgtagccg acgcgaggaa
     1501 atttactcta ggtcttgccg actggctgaa acgcgaagcg tccgtcgact tccgcctgcg
     1561 cgaaacggcg gcaaggctgg ttgtggaagg gaaccgcgtt agcaaggtcc tgaccgagcg
     1621 gaatgaatac aacgccgacg cagtcgtcgt ctgcctaggc accggcagta cgaaattgct
     1681 cgagcctcat ggaatcgacc ccgctatctg cccggtgcgt ggttacagca tcacgctgcc
     1741 tctggggccg catgcgccat cggcaagcat ctccgaccca ggcaggcgga ttgtgctgag
     1801 caggctcggt tcttccatac gcattgccgg tttcgcggac tttgtcggct acgagacgcg
     1861 gcacgacgag gccaggctgg atgccctggt ggcgatggcg cgacgcgtcg cgccgcttgc
     1921 tgccgattac gatgtcggcg acatgcagcg gtggggcggc ttgcgtccga tgacgcccga
     1981 cgggcggccc cgggtcggcc cgacacgggt cggcggcctg ttcctcaata cgggacatgg
     2041 aatgctgggc ttcactttgg ctttagcgac cggggaggca gtttcggagc aggtatctgc
     2101 ccggtcctga tgctttccag gctggcgccg gactgctcaa ccgtcgtcgg ccgttcctgc
     2161 gaggtccgac ggcgacgtcg gagtcgacag catttccgca aatcgcctcc atcgcagctc
     2221 gattgcactt ccctgtccgt tgccgtccgt tcccgtgtcg cgctccacgt agtcacgcac
     2281 gaggtcctgg ccgagattgt agttgatgac gtagctgcgg taggtgtcga agaagtccac
     2341 tctttggcgt gctcgctccg gcgatgaaag cgcatattcc tcaagccatt ccgccgcctg
     2401 ttcgcgggta atattcccgt tcaaataatc gcgcgcggcc tcgttcccgg cgtagctcaa
     2461 ctcatccagc aggtcctgca aggcgtaata gcggtccgcc tcggcgggat cgagccccgc
     2521 cagcgggaac agctcggctt tctcgaattg cacccgctcg tcgtccggga aagccagttc
     2581 cacgccatag ttgccgctac cctcggcgat cagcgaggcc gggctgaaca gcgggtacag
     2641 cgtgaactcc acccatccct cccgctcgac caggtttttc tccagcagca cgttgaacgt
     2701 atggtgcccc gggtaccctt catgacaacc gaggtcgatt gctcgactga tgaaaatcgg
     2761 caggtccgtg ttgatctgga tgaggctctg cgaccgcccc tggtaccagt tgtagcccga
     2821 ccatggcttg tcggtgacat actcgaggct gaacgactcg ttctcgggga ggtttatccg
     2881 ttcgagcgtc cggcgccggc actctgcgat agcggcatcg aacactgaag cgagcctatc
     2941 cgacggaatc acaaagcggt tctggaaatc gttgaccctg gctgaaaggc tgccgtcgcc
     3001 gggcaggagc gcgccgatgc gttccaggat ttcctcgaaa tgggtcgcgt catagtccgg
     3061 tgcggttact ccgaagagca gccgggactc ttcatcgaat gaaaagtccc ggccctggtt
     3121 gatcgccact ctcgtcgaga gcgcgcgcag gcgttgttgc aggccatcga tccgtttgag
     3181 ttgcatcctg ttgctgcggt tgccaggcca cgtcgccacc cggttcgcga gtgcctcggc
     3241 ctcgtcggcg atctgaggca aggtgagggc tgcatcttcc gcggcctgcc gtaagtcttc
     3301 cggaccgtac caggcatcga cgtggttgcc gtcgtgcaat cccatcgcca gttcgaggac
     3361 gacatagttc cgcgcgaggt cgtcgagatc cggagccgac tccatgcgcc gttcttcccc
     3421 acagccggca accgccagcg aggccgctac ggaggtggcc gccgcgagca tacgaacgct
     3481 gatcctggaa ttccggtgca tgaagtctga caggatagca gccgaagctc gttcttttcc
     3541 aaatgctaaa cctgttagga tgtttactgt ccaatcaaac cagcagcccg ccggacgcgc
     3601 gactgctcca ctgatcgcga aggcccgcgg ggctgctgcg gctgatcagc gatgcgtcat
     3661 tcggcgcgga tccggaacgg tgactttagg agtacttcgc gatgcgcaaa gacgtacttc
     3721 agtggctcat tcccgttttg ctggcaatct tgctggcggc ggcgctggtg ttttactggc
     3781 ggcaaagcaa ccgtcccgcg ggcgacatag aaccgaacct ccgcacggat cctccggaaa
     3841 ccacgcagga agcgccgccc gccgcgcccg agcatccgct ggagacgact ggcgaagcag
     3901 cagccgaaca gccggagctg cggccgctgc cacccctgga ggagagcgac gagtacttca
     3961 gcctcgaaat ttcggagctg tttggaccag cactctcgga gaagctgatc gagcaaaacc
     4021 tggttgaacg cgttgtggct acgatcgaca acctgccgcg ggagcagatc gcggacagga
     4081 taaagccgat gggcggcgtc gggggccagc tggtggtgaa cgaaggagaa gaagaagggg
     4141 agtacgtcct ggctccggaa aattacgcgc gttacaacgc gctcgtcggc atggtcgcga
     4201 actcggatat cgccgaggtg gaagaactgt atcgccggtt cttcccgctg ttccagaacg
     4261 catacgtcaa cctgggctat ccggatggct acttcaatga tcgggtaatc gaagttatcg
     4321 accaccttct ggacacaccg gaaccggagg gcgaactaat ccttacccgg ccgcacgtgc
     4381 tctacgagtt cgccgatccg gaactggaag acctttctcc cgggcaaaaa ctcctgctcc
     4441 gcatgggagg tgaacaccgg gcaacggtca aggaaaaact gcgggaattc cgtgcccgca
     4501 ttgtgtcgga ataaagagaa cataaagggg atttcatgcg gttcctggta ggcgcgcttt
     4561 ttatgccggc cactctcgca attgcggccg tgaatcacgt cgggaccggg agcgtacctc
     4621 ctatggccgg gttcgacgaa caggcggccc aggcgcagcg cacgctcgaa aaagcctttg
     4681 acgaagacct gagtgcggcc gaccaggatg aatggctgcg cgacctgtcg gccaggcctc
     4741 atcatgtcgg ctcacgggcg ggccgtgaaa acgcccgtta catagccgac ctgttcgagt
     4801 cctggggtta cgacgtggag atcgaggaat acgggattct cctgccgacg cccgagaccc
     4861 gcgagctgga actgcttgcg ccatacgcgt tcaccgcatc gctcgaagag gacgcgttgc
     4921 cgcaggatcc ctccacctct gtacggggcg agctattgcc gccgtacaac gcattctcca
     4981 ccgatggcga agtggaagga gaactcgtct tcgtcaacta cggcatcccc gaagattacg
     5041 agttgctcga gcggcacggc atcgacgtgc gcggcaaaat tgcgatcgcg aagtatggca
     5101 ggtcgtggcg cggcatcaag ccgaagctcg ccggagaaaa aggcgcgatc ggcaccatca
     5161 tctattccga tcccgccgac gacggcta
//
LOCUS       DASUOM010000326         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1072095,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000326 DASUOM010000000
VERSION     DASUOM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072095"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFY27_06920"
     CDS             complement(<1..688)
                     /locus_tag="VFY27_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5765282.1"
                     /translation="MRSENLRGIVLMLLAMAALSLMDATMKELVGHYSPLQVAALRGI
                     VSLPFVIAWVYWRERTFRTLFHVRWRWHIARGVLAVLMLTSFIYAISGMPLSEAYTLF
                     FVSPLLITALSVPLLKESVDLKRWIAVLVGFGGVLVVLRPGFASLDLLSVAVLLAATC
                     YALNAISVRILGRTDSTAAMSFWFIAMIAVGAGALALPDWQAVRSSDALWLIGMGVTG
                     ALGQLLITEAF"
     gene            complement(750..1469)
                     /locus_tag="VFY27_06925"
     CDS             complement(750..1469)
                     /locus_tag="VFY27_06925"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX5765283.1"
                     /translation="MSYVEGFVAAVPAANKETYRKHAAEAAPLFKEFGATRMVETWGD
                     DVPDGKVTDFKGTVKAKDDEVVVFSWIEYPSREARDAANVKIMTDPRMKEMGSAMPFD
                     AKRMIFGGFAPFVDERAEGSMGYADGYLVPVPADSKDAYRDLAALAATVFKECGATRV
                     VEAWGDDVPEGKVTDYRRAVKAKDNEVVVFSWAEWPSKEARNEGWKKVMADPRMQPGA
                     NAMPFDGQRMIYGGFEPILDA"
     gene            complement(1606..2472)
                     /locus_tag="VFY27_06930"
     CDS             complement(1606..2472)
                     /locus_tag="VFY27_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5765284.1"
                     /translation="MKQKIRYVKTTDGVAIAWARSGQGRTLVRASNWLTHLEYDWQSP
                     VWRHWTRFLATNFDYVRYDERGCGLTDWKVSGLSVERWVADLDAVVEASKVKKPTLLF
                     GVSQGAAVAIAYAVKYPERVSGLILYGGYARGARRRDPDFAIAHDAMQELVRIGWRDP
                     NPVFRQLFTARFIPDGTEEQVTWFNDLCQRTTTPEAAYQLLKARSDVDICDLLQRVRA
                     PTLVIHGDGDEVVPVSEGHLLASEIPGATFVSLASRNHVLLEHEPAWQDFKDAVLQFT
                     AASLPEAVPLPQ"
     gene            complement(2699..>3096)
                     /locus_tag="VFY27_06935"
     CDS             complement(2699..>3096)
                     /locus_tag="VFY27_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765285.1"
                     /translation="KQVQRELFERLFSDQPVDEYLAGVVQQVRSGELDEALVYRKNLR
                     KAVDAYTASTPPHVAAARKSTQPPGRLISYVITTAGPEPLDNRRHPLDREHYVARQVR
                     PVAEPVLETLGLDFERVIGDSRQADLFSF"
BASE COUNT          567 a         1018 c          946 g          565 t
ORIGIN      
        1 tgaaggcttc ggtaatcagc aactggccga gcgcgcctgt tacacccatg ccgatcagcc
       61 acagggcatc gctgctgcgg accgcctgcc agtccggcag cgcaagcgcg ccggcgccga
      121 cggcgatcat ggcgatgaac cagaaagaca tcgcggcggt tgaatcggtg cgcccgagaa
      181 tgcgcacgct gatggcattc agcgcatagc aggtggcggc gagcagcacc gcgaccgaca
      241 gcaagtccag gctggcaaac ccgggccgca ggacgaccag cacgccgccg aagccgacca
      301 ggaccgcgat ccagcgcttc aggtcgaccg actctttcag caagggcacg gatagcgccg
      361 ttatcaacag cggagaaacg aagaacagcg tgtacgcttc tgacagcggc ataccactga
      421 tcgcgtaaat aaagctggtc agcatgagca ccgccagtac gccacgggcg atgtgccatc
      481 gccagcgtac gtggaacagt gtcctgaacg tccgttcgcg ccagtacacc caggcgatca
      541 cgaacggcag cgacacaatc ccgcggagcg cagccacctg tagcggcgag taatggccga
      601 cgagctcctt catggtcgcg tccatcagcg agagcgcagc catggcgagg agcatgagga
      661 cgatgccccg gaggttttcg ctgcgcacgg tatctcttcc ttgcaggcaa ggcggggtat
      721 cttatcggag tggcgggaag ggcttgcgtt tacgcgtcga ggatcggctc gaagccgccg
      781 tagatcatgc gctggccgtc gaacggcatg gcgttcgcgc caggctgcat gcgtgggtca
      841 gccatcacct tcttccagcc ctcgttacgg gcctccttcg acggccattc ggcccaggaa
      901 aagacgacaa cttcgttgtc ctttgccttt accgcgcgcc tgtagtcggt caccttgccc
      961 tccggcacgt cgtctcccca cgcctcgacg acgcgcgtgg cgccgcactc cttgaacacc
     1021 gtcgcggcga gggccgccag gtcccggtat gcgtccttgc tgtccgcagg aaccggcacg
     1081 aggtaaccat cggcgtaacc catgctgccc tcggccctct cgtcaacgaa gggcgcgaac
     1141 ccgccgaaga tcatccgctt tgcgtcgaac ggcatggccg agcccatctc cttcatcctc
     1201 gggtcggtca tgatcttcac gttcgcggcg tcgcgcgcct ccctggacgg atattcgatc
     1261 catgagaaga cgacgacctc gtcatccttc gccttcaccg tgcccttgaa gtccgtgacc
     1321 ttgccgtcgg gcacgtcatc gccccatgtc tccaccatgc gggtcgcgcc gaactccttg
     1381 aacagtggcg cggcctcggc ggcgtgcttg cgatacgttt ccttattcgc ggcgggtacc
     1441 gctgcgacga atccttctac atagctcatt gctcatctcc tgatcagtca tgtcggcaac
     1501 attcccggag gcctgtggct acccggtctg tgaaatagtc gaaccctgct tcgccaaatc
     1561 gacaccgccg gaggagcaag cctgaaccag gctggcgcgg ctgcgctact gcgggagcgg
     1621 tactgcttcc ggcagcgagg cggcggtgaa ctgcagcacg gcgtccttga agtcctgcca
     1681 tgcgggctcg tgttccagca gcacatggtt gcgcgacgcc agggatacga acgtcgctcc
     1741 gggaatttcg ctcgcgagca ggtgaccttc gctgacaggg actacctcgt cgccgtcgcc
     1801 gtggattacc agcgtcgggg cacgtaccct ttgcagcagg tcgcaaatgt cgacgtccga
     1861 acgggctttc aacaactggt aggccgcctc cggcgtggtg gtgcgctggc acaggtcgtt
     1921 aaaccaggta acctgctctt ccgtgccgtc ggggatgaag cgcgcggtga acaactggcg
     1981 gaagaccggg ttcgggtcgc gccagccgat gcgcaccagt tcctgcatcg cgtcatgagc
     2041 aatcgcaaag tccggatctc gcctccgcgc gccccttgca tagccaccgt acaggatcag
     2101 gccggagacg cgctccgggt acttgacggc ataggcaatg gcgaccgctg cgccctggct
     2161 cacgccgaac agcaacgtcg gcttcttcac cttcgacgcc tcgacgacgg catccaggtc
     2221 cgccacccac cgctccaccg aaagaccgct aaccttccag tcggtcaggc cgcaaccgcg
     2281 ttcgtcgtac cggacgtagt cgaaattggt ggcaaggaaa cgcgtccagt ggcgccagac
     2341 ggggctttgc cagtcgtact cgaggtgcgt cagccagttc gaagcgcgaa ccagcgtcct
     2401 gccctgcccg gaacgggccc aggcgatggc gacgccatcg gtggtcttca cgtatcggat
     2461 tttctgtttc atctggacag ggacgccgcc gttatgtccc gttcggatgc ctattgtgcc
     2521 aaaaggatcc ctaggagtct gcgcggtaga aagtattcct actgcgaacg cgccgcagcg
     2581 gcccattttt ccgatttttc tcgttgcgta gggcccctat gcgcctcaaa agatcgaaaa
     2641 aatggactcg ctgtggcacg ttctcgctac ggatatttct accgcgcaga ctcctagctt
     2701 aaaacgagaa aaggtcggcc tggcggctgt cgccgatgac gcgctcgaaa tcgaggccca
     2761 aggtctcgag caccggttcg gcgaccggcc tcacctgcct ggccacgtag tgctcacggt
     2821 cgagcggatg ccgccgattg tccagcggct ccgggcctgc ggtcgtgatg acgtagctga
     2881 tcagccgccc cggaggctgc gtcgacttgc gtgcggcagc cacgtgaggg ggagtgctgg
     2941 cggtatacgc gtcgacggct ttgcgcaggt tcttgcgata cacgagcgcc tcgtccagct
     3001 cgccgctccg cacctgctgg acgacccctg ccaggtactc gtcgaccggc tggtcggaaa
     3061 acaggcgctc gaagagctcg cgctgcacct gcttcg
//
LOCUS       DASUOM010000327         7362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1100643,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000327 DASUOM010000000
VERSION     DASUOM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100643"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1521
                     /locus_tag="VFY27_06940"
     CDS             121..1521
                     /locus_tag="VFY27_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX5765286.1"
                     /translation="MLKEEAAGKAPRTSLHGHWSSRMAFILAVTGSAVGLGNIWKFPY
                     LAGVNGGGAFVLVYLLCVVGIGMPVMMSEILIGRRGRRNPVATMGLLGEEEGRSRHWR
                     LVGGMGVLAGILILSYYSVIAGWTLAYTWKSGAGTFAGATPELVASTFGGFTQNWVSV
                     GISHTLFMAITVWVVARGVQRGLEQAVRFMVPALLLLMLVLLGYSINSGSFSAGVAFL
                     FTPDFGALTWDSVLVAMGQAFFTLSIGMGAVMAYGAYLPAETSITGASASVVTADTTI
                     ALLAGLVIFPLVFANGLDPGEGPGLVFFTLPLAFGQMTGGVFFGTIFFVLLVFAAWTS
                     AIGLMEPAVAWLVETHSRTRAQAALMVGTLIWALGFGTVLSFGTLADFRFYKGTIFDN
                     VDHVTSNIMLPLGGVFITVFAAWAMCRNSSAEELHGAGALYKLWRISARYVAPIAIIF
                     VFLKAVGVLPELSSGG"
     gene            1546..1983
                     /locus_tag="VFY27_06945"
     CDS             1546..1983
                     /locus_tag="VFY27_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX5765287.1"
                     /translation="MRKVNRSALVPYSAREMFSLVDDVESYPQFVPWCNDATVERRSG
                     ESVVATLELHKGPISKHFTTRNTPRNFDAIDMELVGGPFRQLSGGWRFRDIEGQGCRV
                     SLELEFEFEGRLVDMMFGAFFKDAGDSLVDAFIKRAREVYGPR"
     gene            1987..2328
                     /locus_tag="VFY27_06950"
     CDS             1987..2328
                     /locus_tag="VFY27_06950"
                     /inference="COORDINATES: protein motif:HMM:NF002490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX5765288.1"
                     /translation="MTPGNSGESLRIEVVFALPDRQELVTVTVGEGATVEHAIARCSL
                     RASFPDWDLENCAVGIWGRPVRREQPLQDGDRVELYRTLKIDPREARRALAAQGRSMG
                     NFPDNRRRRED"
     gene            complement(2325..2651)
                     /locus_tag="VFY27_06955"
     CDS             complement(2325..2651)
                     /locus_tag="VFY27_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX5765289.1"
                     /translation="MRKLLVTLLSILTLSLATGCVYQASLSQGNLLKQADLDQVKVGM
                     TRNQVRFLLGTPMIDDPFHEERWDYVYYLRVGREKATFKRWVSIYFDGDTVSEIVDDQ
                     ELEPDL"
     gene            2838..3257
                     /gene="fur"
                     /locus_tag="VFY27_06960"
     CDS             2838..3257
                     /gene="fur"
                     /locus_tag="VFY27_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX5765290.1"
                     /translation="MFQRQELRKAGLKVTLPRVKILEILESNRQRHLSAEDIYKELLE
                     TGEDIGLATVYRVLTQFETAGLVKRHNFEGGHSVFEMDEGEHHDHMVCVETGDVIEFM
                     NDEIERLQREIAKEHNFELIDHSLVLYVRPRKKGENT"
     gene            complement(3320..4999)
                     /gene="recN"
                     /locus_tag="VFY27_06965"
     CDS             complement(3320..4999)
                     /gene="recN"
                     /locus_tag="VFY27_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5765291.1"
                     /translation="MLTRLQVRNFAIIDALEVEFGPGMTVLTGETGAGKSILVDALGL
                     VLGERGGAGLVRSRARRAEFSADFDVSGHPEARTWLEEQAVDADDDCLLRRVIGEDGR
                     SRAFINGNAVPLQSLKELGEMLLDIHGQHFHQSLGRRSVQRDLLDFFGGLQELRASTA
                     EAFADWKTLLERLTQLEQAESDRAARLDLLAFQAQELEALDLAADEFESLQAERQRLR
                     MSGVLAEGVTRALQLIYDDENANAQSLLTSACHELDSGSAIDAGLKQAQEMLEEAAIQ
                     VSEAADVLHRYGDSLEVDPARRDWVEDRIDAIQAVARKHRVEASELVALKNLLHTQLD
                     ELQDAEATGARLAKEASDARAKYLKQATLLSGERKKAATRFAARVTGAMQGLGMPGGV
                     FEVSLTSLSVDDARASGLEEVEYLISANPGQSPMPLARIASGGELSRMSLSIQVIASD
                     GSAIPTMVFDEVDSGVGGSVAEMIGRRLKGLSATRQVFCVTHLPQVASLADHHFRISK
                     VNDGKSTRTEIAELGEEERIEELARMLGGVKITERTREHAAEMLELGNADR"
     gene            complement(5011..5904)
                     /locus_tag="VFY27_06970"
     CDS             complement(5011..5904)
                     /locus_tag="VFY27_06970"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEX5765292.1"
                     /translation="MGKEFSTVALVGRSEDPRVAEPMTALANYLVNAGVSVIASPAAG
                     LEPPVQTVDEEEIAIRANLIIAVGGDGTMLYAAAIGGEKAVPLLGINRGRLGFLADVT
                     PDEMLQSLGKVLDGDYTRETRLLLRAQIISPGNAVRSAAALNDVVMQRRETGRMVDFE
                     TRIAGRYVNTHSGDGLIVSTPTGSTAYALSCGGPIMEPGLDAVVVVPVCPHTLSDRPI
                     VIPSRHRIEVRLLPSSLARAEVTVDGQMLGDFNAEDRLIISESDCRITLIHPPGYDYF
                     EILRSKLHWGRDSRVRGGPRD"
     gene            6062..7120
                     /gene="hrcA"
                     /locus_tag="VFY27_06975"
     CDS             6062..7120
                     /gene="hrcA"
                     /locus_tag="VFY27_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5765293.1"
                     /translation="MNSETISPLPNERAQYLLKVLIQRFIRDGQPVGSRTLSRDSGLD
                     LSPATIRNVMADLEELGFVSSPHTSAGKVPTPRGYRLFVDTLVRYKQPKNDDIRKLQA
                     QFEKQFHNSDEMLDAVSNLLSKITSMAGVVTIPRAHQALLRQIEFLPLSEKRVLAILV
                     INDREVQNRILHIEQEYTATELQRAANYINENYAGTDLLQIRERLISDLESTRDSMNR
                     AMHDIIAVAQAAMHETTPKNEFLLAGETNLMDFAELSDLDTLRRLFEAFSRKRFMLDL
                     LDRSINAKGVQVFIGEESGFQILDNCSVVTAPYNVDKETVGVLGVIGPTRMAYDRVVP
                     IVDVTARLLGTALKAAIN"
BASE COUNT         1372 a         2241 c         2210 g         1539 t
ORIGIN      
        1 gattatacgg cagtcggttc tcgcgatcct ccgggcgagg ctgttgcgtc cgtttgccgt
       61 ctgttgtcag tgatttcgtt ttcctccaga atccccgcct ctccgggtac tgagacgaca
      121 atgctaaaag aagaagcagc cggcaaggcg ccacgtacct ctcttcatgg ccactggtcg
      181 tcccgcatgg catttatcct tgcggtaacc ggttcggcag tcgggctcgg caacatctgg
      241 aagtttccgt acctggccgg tgtcaacggc ggtggcgcgt tcgtcctggt ttaccttctg
      301 tgcgtcgttg gcatagggat gccggtaatg atgtcggaga tcctcatcgg ccggcgcggc
      361 cggcggaacc cggttgcaac gatgggcttg ctgggagagg aggagggacg gtcgcggcac
      421 tggcgactgg tcggcggcat gggtgtcctg gccggtatcc tgattctttc gtattacagc
      481 gtcatcgccg gctggacact tgcgtacacg tggaaaagcg gtgccggaac gttcgcaggg
      541 gcgacccccg agctggtagc gtcgaccttc gggggcttca cacagaactg ggtgtcggtc
      601 ggcatcagcc ataccttgtt tatggcgata actgtctggg tcgtggctcg cggtgtccag
      661 cgaggcctcg agcaggcggt tcgcttcatg gtgccggcac tgctgctact gatgctggtt
      721 ctcctgggct actcgatcaa ctccggctct ttctccgccg gggtggcgtt cctgtttacg
      781 ccggacttcg gagcactgac gtgggacagc gtactggtcg ccatgggcca ggcgttcttc
      841 acgttgagca tcggtatggg cgccgtaatg gcctacgggg cgtatcttcc tgcggaaact
      901 tccatcaccg gcgcgtccgc gtcggtagtg acggcggata cgaccattgc gctcctcgcc
      961 ggtctcgtga tcttcccgct ggtgttcgcg aatggtcttg atcccggcga aggccccggc
     1021 ctggtttttt tcaccctgcc gctcgctttc ggccagatga ccggcggcgt gttcttcggc
     1081 acgatcttct tcgtcctgct ggtctttgcc gcctggacct ccgcgatagg cctgatggag
     1141 ccggcggtcg catggctggt agagacccat agccgaaccc gggcacaggc cgccctgatg
     1201 gttggcacgc tgatttgggc gctggggttc ggcacggtgc tgtcattcgg cacgctcgcc
     1261 gatttcaggt tttacaaggg cacgatcttc gacaacgtcg atcatgtgac gagcaacatc
     1321 atgctgccgc tcggaggtgt cttcatcacc gtatttgccg cttgggccat gtgccgcaat
     1381 tcgagtgcgg aggagctgca tggtgccggt gcgttgtaca aactgtggcg aatctccgcc
     1441 cgctatgtcg cgccgattgc aatcatcttc gtgttcctga aagctgtcgg tgtgctgccg
     1501 gagctttcct ccggcggatg atgcagggga ttccgaacag aacccatgcg taaggtcaat
     1561 cgaagtgcgc tcgttcccta cagcgcgcgc gagatgttca gcctcgtgga cgacgtagag
     1621 tcttatccgc agtttgttcc atggtgcaat gatgcaacgg tggagcgccg ctcgggcgag
     1681 tcagtcgttg cgacgctgga gttgcacaag ggacccatca gcaagcattt cacgacgcgc
     1741 aacaccccca ggaattttga tgcgatcgat atggaactgg ttggcggacc tttccgtcaa
     1801 ttgtctggcg ggtggcggtt ccgggatatc gaagggcagg gttgccgcgt gtcgctggag
     1861 ctggagttcg agttcgaggg caggctggtg gacatgatgt tcggcgcgtt cttcaaggat
     1921 gccggggatt ctctcgtgga tgcctttata aaaagggcaa gggaagtgta tggcccacgg
     1981 tgaccgatga cgccaggcaa ttcaggcgag tcgcttagaa tcgaagtcgt gttcgcgttg
     2041 cctgaccggc aggagctggt gaccgtgacg gttggcgaag gtgcaacggt cgagcatgca
     2101 atagcgcgct gctcgctcag agcctcattt ccagactggg acctggaaaa ttgcgctgtc
     2161 ggtatctggg gtcgcccggt acgacgtgaa cagccgctgc aggacggaga ccgcgtggaa
     2221 ctctacagga cattgaaaat cgacccgcgc gaagcccggc gagctctggc ggcgcagggg
     2281 aggtcgatgg gcaactttcc cgacaaccga cgtcgccggg aggattaaag atcaggctcc
     2341 agttcctggt cgtctacgat ctcactcacg gtgtcgccat cgaagtagat cgaaacccag
     2401 cgcttgaagg tcgctttctc gcgcccgacc ctgaggtagt agacatagtc ccaccgttcc
     2461 tcgtggaacg ggtcgtcgat catcggcgtg cccagtagaa aacgcacctg gttgcgtgtc
     2521 atgccgacct tgacctggtc gaggtcggcc tgcttgagca ggttgccctg tgaaagtgac
     2581 gcctggtaaa cgcagcctgt tgcaagcgac agtgtcaata tggacagtaa ggtaacaagg
     2641 agttttcgca ctgtgtcctt cgcttaaaga tgttccggtg acttcccctg catggcggga
     2701 ctcccgcaag tgacactccg cgatcagtcc ggtggagcgg cctttccatc tgggtcataa
     2761 taggcgcgga tcatacctgc tcgcccccgg ccgcggtagt tgcgccggga tcgtcctgca
     2821 acgaaggacc tcagtgcatg tttcaacgtc aggaattacg caaggccgga ctcaaagtca
     2881 ccttgccgcg tgtaaagatt ctggaaatcc ttgaaagcaa ccgccagcgt cacctgagcg
     2941 cggaagacat ctacaaggaa cttctggaaa caggcgagga tatcggcctt gcgaccgtct
     3001 accgcgtact gacgcagttc gagacagcgg ggctggtgaa acgccacaac ttcgagggcg
     3061 ggcattccgt cttcgagatg gatgaaggcg agcatcacga tcacatggtg tgcgtcgaga
     3121 cgggtgatgt gatcgaattc atgaatgacg agattgagcg gctgcagcgc gagatcgcta
     3181 aagaacacaa cttcgagctg atcgaccaca gcctggtgct ttacgtccgt ccgcgcaaga
     3241 agggcgagaa cacctaggag atccggaaga cttgcaggag ccccgtgggc ggccgggtag
     3301 cgggctaaac gggtggctcc taccggtctg cgtttcccaa ttccagcatc tctgccgcgt
     3361 gttcgcgggt acgttcggtt atcttgacgc cgccaagcat tcgcgcaagt tcctcgatgc
     3421 gctcctcttc gccgagctcc gcaatctccg ttcgtgtcga cttgccgtcg ttcaccttgc
     3481 tgatacgaaa atgatgatcg gccaggcttg caacctgggg caggtgggtt acgcagaata
     3541 cctggcgggt tgcgctcagg cctttcagtc gccgtccgat catctcggcg acgctgccgc
     3601 ctacgccgct gtcgacttcg tcgaacacca tggtgggtat cgcgctgccg tcgctcgcga
     3661 tgacctgtat cgaaaggctc atgcgggaga gttcgccacc ggatgcgatc cttgcaagag
     3721 gcatagggct ctgcccggga ttcgcgctga tcaggtactc gacctcttcc aggcctgatg
     3781 ctctcgcatc gtctacgctc aaagacgtaa gcgacacctc gaacacccct cctggcatcc
     3841 cgagaccttg catcgccccg gttacgcggg cggcgaaccg ggtggcggct tttttgcgtt
     3901 cgcccgagag cagggtcgcc tgcttcaggt acttcgctct tgcatccgag gcttcctttg
     3961 cgagccgtgc gcccgtggct tcggcatcct gcagttcgtc cagttgcgta tgcaggaggt
     4021 tttttagcgc caccagttca gaggcttcaa cccggtgttt tcgcgccacc gcctgtatcg
     4081 catcgatccg atcctccacc caatcgcggc gggccgggtc aacctccagc gagtcgccgt
     4141 aacggtgcag cacatctgcc gcctcggaga cctggatagc agcctcctcg agcatttcct
     4201 gcgcttgctt cagcccggca tcaattgcgc tgccggaatc cagctcgtga caggcagacg
     4261 taagcaggct ttgcgcgttg gcgttctcat cgtcgtatat caactgcaac gcgcgggtga
     4321 caccttctgc gagaacgccg ctcatcctca gtcgctggcg ctctgcctgc agcgactcga
     4381 attcgtctgc cgcgaggtcc agggcctcca gctcctgcgc ctggaaggcg agcaggtcaa
     4441 ggcgtgcagc acggtcgctc tcggcctgtt cgagctgcgt gagcctttcc agcagggttt
     4501 tccagtcggc gaatgcctcc gctgtggacg ctcgcagttc ctgcagcccg ccgaaaaaat
     4561 ccagcaggtc acgctgcacg ctacggcgtc ccagcgactg gtgaaagtgc tggccatgga
     4621 tatccagcaa catttccccc agttctttca ggctttgcag cgggaccgca ttgccgttga
     4681 tgaacgcgcg ggatcgccca tcctccccga ttacgcgccg gagcaggcag tcgtcatcgg
     4741 catccacagc ctgttcttcc agccaggtcc ttgcctcagg gtggccggag acatcgaagt
     4801 cagcgctgaa ctcggcgcgc ctggcgcggc tccggacgag gcctgcgccg ccgcgctctc
     4861 cgagcaccag gccgagcgca tcgacaagga tggatttgcc ggcgcctgtc tcgccggtca
     4921 ggaccgtcat tccagggccg aattccacct cgagggcatc tatgatcgcg aagttccgga
     4981 cctgcaggcg tgtcagcatc ggagcgagaa tcaatcgcgt ggaccgccgc gtacgcggct
     5041 gtccctgccc caatgcagct tggagcgcag tatctcgaag taatcgtagc caggcgggtg
     5101 gatcaacgtg attcggcagt cggactcact gataatcagg cggtcctccg cattgaagtc
     5161 ccccagcatc tgcccgtcga ccgtcacttc cgcgcgcgcc aggctcgatg gcagcagccg
     5221 aacctcgatc cggtgccgcg agggaatgac gatcggccgg tcgctgagcg tgtgggggca
     5281 gacgggcacg accactaccg catccagtcc cggctccatg atcggaccgc cgcagctcaa
     5341 ggcataggca gtcgacccgg tgggcgtcga cacgatgagg ccgtcgcccg aatgcgtatt
     5401 gacatagcgc cccgcaatgc gggtttcgaa atccaccatt cgcccggttt cgcgccgctg
     5461 cattaccaca tcattcaagg ctgccgccga tctcacagcg ttacccgggg agatgatctg
     5521 ggcgcgcagc aacagccgcg tttcgcgcgt gtaatctccg tccagcacct tgcccaggct
     5581 ttgtagcatt tcatccggtg tcacgtcggc aaggaagccg agccggcccc ggttgatgcc
     5641 aagcagcgga actgccttct cgcccccgat ggccgccgca tataacatcg ttccgtcacc
     5701 gcctaccgca atgatgaggt tcgcgcggat ggcgatttct tcttcgtcca ccgtttgcac
     5761 cggcggctcc agccccgcag cgggagatgc gatgacactg acgccggcgt tgacgagata
     5821 atttgcgagc gcggtcatcg gctcggcaac gcgcgggtct tccgatcgtc cgaccagcgc
     5881 caccgtgctg aattcttttc ccatgtgcga gtcaaattta tcgagcgatg agccaggcac
     5941 gcaggcgatc ccggcgggag cgcccattgt tgcacgattg ttgcaaaccc ttgacccacc
     6001 gccaagccgt tgttagcttt ttgttttccg gtcgcaaatg caggttgcgt agcaccagcg
     6061 aatgaactcc gagacaatca gtcccttacc gaacgaacgc gcgcagtacc tcctgaaggt
     6121 actgatccag cgattcatcc gcgacggaca gcccgttggc tcacggactc tgtcgcgcga
     6181 ttccgggctc gacctgagtc ccgccacgat tcgaaacgtc atggccgacc tcgaggagct
     6241 gggcttcgtc tcgtcccctc acacctctgc aggcaaggtt ccgacaccgc gagggtatcg
     6301 gctgttcgtc gatacgctgg tccgctacaa acagccgaag aacgacgaca ttcgcaagct
     6361 gcaggcgcag ttcgaaaaac agttccacaa ttccgacgag atgctggacg ccgtttcgaa
     6421 cctgctctcg aaaattacca gcatggcagg cgtcgtcacc atccctcgtg cgcaccaggc
     6481 gctgctgcga cagattgaat tcctgcctct ttccgagaaa cgcgttctcg cgattctcgt
     6541 catcaacgac cgagaagtgc agaaccgcat tctgcacatc gagcaggagt acacggctac
     6601 ggaactgcag cgcgcggcaa attacatcaa tgagaactat gccggaaccg atttactgca
     6661 gattcgagag cggctcatat ccgacctgga aagcacgcgg gattccatga accgcgccat
     6721 gcacgacatc atcgctgtgg cacaagccgc aatgcatgaa accacgccaa agaacgagtt
     6781 tctgctcgcg ggcgaaacga acctgatgga tttcgcagag ctgtcggatc tcgatacgct
     6841 gcgaaggctc tttgaagcct tctccaggaa gcgcttcatg ctggacctgc tcgaccgcag
     6901 cattaatgca aagggggtcc aggttttcat tggcgaggaa tccggcttcc agatcctcga
     6961 caactgcagc gtcgtcaccg ctccgtacaa cgtcgataag gaaacggtcg gcgtactcgg
     7021 tgtcatcggc cccacccgca tggcatacga ccgggtggtg cccatcgtgg acgtcacggc
     7081 ccgcctcctg gggacggcgc tcaaggccgc cataaattaa gtaaggccgc atcgcgctcc
     7141 cttaaggcca gccttgatat ttgcggccag gtccccaaac tcccacaatc gtcggatgtc
     7201 ccgcgattgt tcgatgcgcc gggctccgcc ggacaccctg ggtttcgacg tctcgtcgct
     7261 ttcccgattc gattcaatat ggagcaatga accagatgag cgttaatccc gattcttcgg
     7321 acgaccagcg ttacgacagc cccacgaacg gcgccgacag ag
//
LOCUS       DASUOM010000328         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1467141,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000328 DASUOM010000000
VERSION     DASUOM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467141"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFY27_06980"
     CDS             <1..645
                     /locus_tag="VFY27_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX5765294.1"
                     /translation="NAVLPIRQFDADCFVFMATSTGRVKKTPLSLFSRPRASGIIAID
                     LRADDRLVDVAITDGKQEIMLFSSNGKAIRFSEDDVRPMGRGAAGVRGLKLEPGHDLI
                     ALLIVGEGLVLSATENGFGKRTAVDEFPIQGRGGRGVIGIQTTERNGRTVGALQVNED
                     DEIMLISSSGTLVRTPVSDISIIGRNTQGVRLIRVEAGERLVGLAKIEAMVEEE"
     gene            715..1791
                     /gene="serC"
                     /locus_tag="VFY27_06985"
     CDS             715..1791
                     /gene="serC"
                     /locus_tag="VFY27_06985"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX5765295.1"
                     /translation="MSRVFNFSAGPAVLPEPVLEQVRADIPDWHGGMSVMEVSHRSKE
                     FIAVAESAEQDLRELLSIPDNYAVLFVQGGATMQFSMVPLNLAGPGDTVDYVQTGAWS
                     KKAIAEARRFCNVNIAADSSDSNFAHIPPESNWRLSDGAAYLHYTPNETIGGVEFHFI
                     PDTAVPLVADMSSTILSRPIDVSRFGVIYAGAQKNIAPAGITVVIVRRELLGRERENV
                     PTLLTYKVYAESGSMSNTPPTFTWYVAGLVFKHLKAQGGLEAVAERNARKAAKLYDAI
                     DASSYYSNPVSKEARSWMNVPFVLRNPSADAKFLSEADAAGLRNLKGHRSVGGMRASI
                     YNAMPEEGVDRLIQFMRDFERVNA"
     gene            1841..3019
                     /locus_tag="VFY27_06990"
     CDS             1841..3019
                     /locus_tag="VFY27_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5765296.1"
                     /translation="MYKIRTLNSIAIAGLRRLPRERYEVSSDISRPDAIIVRSADMHS
                     MEIPDTVAAVGRAGAGVNNIPIQALSNRGVPVFNAPGANANAVKEMVAASLLLAARNI
                     CNARDYVRKLDGEGDSLNSAVETGKKQFVGFELPGRTLGVIGLGAIGVEVANVALALG
                     MKVVGFDPKITVRRAWQLSSAVQQVDTLDRLFKKSNAITLHVPLIEATRHLVNRDRLA
                     LMPPKSVLINFARGGVVDNEAAIAALDSGQLHAYVCDFPTPELIAHPKVVAMPHLGAS
                     TTEAEENCAIMVAETIRDYLENGNIMNSVNFPEAVMPRANAHRISIANANVPNMVGQI
                     STCLAHAGLNIEDLLNKSLGDLAYTIVDLDGPVPDETIAALRKIKGVLALRSLGKPIS
                     "
     gene            3033..>3344
                     /gene="pheA"
                     /locus_tag="VFY27_06995"
     CDS             3033..>3344
                     /gene="pheA"
                     /locus_tag="VFY27_06995"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5765297.1"
                     /translation="MVKAPPQDDNAQLAAMRRRIDEVDQQIQDLLNERATYAQQVGLT
                     KGELASAVDYYRPEREAAVLRAVQARNKGPLRDEEMLRLFREIMSACLAQQEPLKVGF
                     LG"
BASE COUNT          716 a          964 c         1047 g          617 t
ORIGIN      
        1 aatgcagtcc tgccaatacg gcagttcgac gccgattgtt tcgttttcat ggccaccagc
       61 accgggcgtg tcaagaaaac acctctgagc ctgttttcgc gtccgagggc gagcgggatc
      121 atagcgatcg accttcgggc cgatgatcgg ctggtcgacg tcgccattac tgacggcaag
      181 caggagatca tgctgttctc cagtaacgga aaggccatcc gcttcagcga ggacgacgtc
      241 cgcccgatgg gcaggggtgc agccggagtt cgtggcctca agctggagcc cggtcacgac
      301 ctcatcgcgc tgttgatcgt gggcgaaggc ctggttcttt ccgcaacgga gaacgggttc
      361 ggcaagcgca cggcagtcga tgagtttccg attcagggcc gcggggggcg cggcgtcatc
      421 ggcatccaga cgacagagcg taacgggcgg acggttggcg cactgcaggt aaatgaggac
      481 gacgagatca tgctgatcag ttccagcgga acgctcgtgc ggacccctgt gagtgatatc
      541 tctattatcg gccgcaatac ccagggcgtg cgcctgatcc gggtagaggc gggcgagcgc
      601 ctggttggtc tggcgaaaat tgaggcaatg gtggaagaag aatagcttct tcctcaccat
      661 atagtccctc gccgtacctc atcccgctcc cggttcaagg agaacgcaga aaggatgtca
      721 cgagtgttta acttcagcgc gggaccggcg gttttgccgg aaccggtcct ggaacaggtg
      781 cgtgccgaca ttccggactg gcatggtggg atgtcggtga tggaagtgag ccaccgcagc
      841 aaagagttta tcgccgtcgc ggaaagcgcc gagcaggatc tgcgggagct gctatcgatc
      901 cccgacaact atgcagtgct gttcgtgcaa ggcggcgcaa cgatgcagtt ctccatggtt
      961 ccgttgaacc tggcggggcc tggcgacacc gtcgattatg tgcagacggg ggcatggtcg
     1021 aaaaaagcta tcgcggaggc gcggcgcttc tgcaacgtca acatcgctgc ggacagcagt
     1081 gacagcaatt ttgctcacat cccgccggag agcaactggc gcctgagcga cggagcagcg
     1141 tacctgcact acacgcccaa cgagacaatc ggtggtgtcg agttccattt catacccgat
     1201 accgcggtgc cgctggtagc cgatatgtcc agcaccatcc tgtcgcggcc gatagatgtc
     1261 tcccgcttcg gtgttatcta tgcaggcgcg cagaagaaca tcgcgccggc aggcatcacc
     1321 gtggtgatcg tccgcaggga attgttgggg cgcgagcggg agaacgtccc cacgctgctg
     1381 acctataagg tctatgcgga gtccggttcc atgagcaata cgccgccaac gttcacctgg
     1441 tatgtcgcag ggctggtgtt caagcacctt aaggcgcaag gcggcctcga agcagtggcg
     1501 gaacgcaacg cgcgcaaggc cgcaaaactg tacgatgcta tcgacgcgtc atcttattac
     1561 agcaatcccg tcagcaagga agcccgctcg tggatgaacg ttcccttcgt gctgcgaaat
     1621 ccctcggccg acgcaaagtt cctgagcgag gcggatgcgg caggcctcag gaacctgaaa
     1681 ggccatcgct ccgtcggcgg catgcgtgct tcgatataca atgcgatgcc ggaagaaggt
     1741 gtggacaggc tcatccagtt catgcgggac ttcgaacggg tgaacgcctg acagcctgcc
     1801 aggaagcgca acaacgttcg ccaaccacaa ccgggaaacg atgtacaaaa tacgcacact
     1861 gaacagcatc gccattgcag gcctccgcag gttgccgaga gagcgctatg aggtttcttc
     1921 cgacatctcg cgcccggatg ccatcattgt tcgctccgca gacatgcaca gcatggaaat
     1981 ccccgacacg gtagccgcgg tcggtcgggc gggtgcgggc gtgaacaaca tcccgatcca
     2041 ggcgctaagc aaccgcggcg tgccggtgtt caacgccccc ggcgccaatg ccaatgctgt
     2101 aaaagagatg gttgcggctt cactgctgct cgcggcaagg aacatctgca acgcaaggga
     2161 ttatgtgcgc aagctcgatg gcgagggcga ttcccttaat tcggcagtag agaccggaaa
     2221 aaagcagttt gtcggcttcg aactgccggg ccgcacgctg ggggtcatcg gtcttggtgc
     2281 gattggcgtg gaagtcgcca acgttgccct tgcgctcggc atgaaggtcg tcggcttcga
     2341 cccgaagatc actgtgcgcc gcgcgtggca attgtcgtcc gcggtgcaac aggttgacac
     2401 gcttgaccgg ttgttcaaga agtcgaatgc aataacgttg catgtgccgt tgatcgaggc
     2461 gacgcgtcac ctcgtcaacc gggaccggct cgcgttgatg ccgccaaaaa gcgtgttgat
     2521 caacttcgcc aggggtggag tggtggataa cgaggcggcg atcgccgcgc tcgacagcgg
     2581 gcagctgcat gcttacgtat gcgattttcc gacgccggaa ctgattgccc accccaaggt
     2641 cgtggccatg ccacatctcg gcgcttccac taccgaagca gaagagaact gcgcgatcat
     2701 ggttgcggag accatcaggg actacctcga gaacggcaac atcatgaact cggtcaactt
     2761 cccggaggcg gtaatgccgc gagcgaatgc gcacaggatc tcgatcgcca atgcgaacgt
     2821 gccgaacatg gtagggcaga tatcaacctg cctggcgcat gcggggctca atatcgaaga
     2881 tcttctcaac aagtccctgg gcgatctcgc gtatacgatc gtcgacctcg acggaccggt
     2941 acccgatgaa accatcgccg cgcttcgcaa gataaagggc gtccttgcct tgcgcagtct
     3001 cggcaagccg atatcctgag cagcacagac taatggtcaa agccccaccc caggacgata
     3061 atgcgcagct ggcggcgatg cgcagacgca tcgacgaagt cgaccagcag atccaggatc
     3121 tgctgaacga gcgcgccacg tacgcgcagc aggtgggatt gacaaaaggc gagctcgcat
     3181 cggccgtcga ctactatcga cctgaacgcg aagctgccgt cttgcgggcg gtccaggctc
     3241 gcaacaaggg gccgcttcgg gacgaggaaa tgctgcggct tttccgcgaa atcatgtcgg
     3301 cctgccttgc gcagcaggag ccgctgaagg tgggtttcct cggg
//
LOCUS       DASUOM010000329         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1483628,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000329 DASUOM010000000
VERSION     DASUOM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483628"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..752)
                     /locus_tag="VFY27_07000"
     CDS             complement(315..752)
                     /locus_tag="VFY27_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765298.1"
                     /translation="MSEAEFEPIQQVVILAIPESESPTTLRAIEPDSGMAMDTTTLAD
                     YSTKTSRGSYEARFRGIGPYRLLPLAELNAFDGGWEGFYQRHPGAAGLFTFSRVGVSK
                     DGQEAIVRVMHLRGGLWGHIDSMLFRRRGDTWHLAGEQRLEDI"
     gene            complement(1242..2075)
                     /locus_tag="VFY27_07005"
     CDS             complement(1242..2075)
                     /locus_tag="VFY27_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765299.1"
                     /translation="MPNVVQTSPDDILVGDARAGKLVRFGADMEFRGTALVASLFPTL
                     AAKFARIDPMGITPDGTPIGVARDQDSVRWYVGDSAAPLVSIPAGGGTGRARIADGML
                     TFVQPFREDPIIIADPGREVILKLDRGSDSLYLEIRSTTDESSNVRHAISSESRPIPA
                     EVVSARIDSFAAYYQGRGIPMDEVRSSIRAAMHVPERSHGVAGIFVSGDSLVAITHEG
                     QGSERSVARVFRYSTGEAVGEFEIPPRSRLVSFSRGHVWLLQRDQHDVEYLVKRTISM
                     R"
BASE COUNT          546 a          923 c          818 g          498 t
ORIGIN      
        1 aggctcaatc gcgaattcgc gagagcagcc cgcgcaagcc aggacgagga gaacgcacgt
       61 aacgccttgt gatcgtctgt gcatgtcgta ccgtgaggcg tttcgcctaa cgttcaagct
      121 ttgagacgtg gcccgcagac caggacctga gcgtcagcga cggtcctggg cgaagggaca
      181 tgtccgccgg tccacacgcc caggcacgcc tactcttcac ccggcggccc aggcggcgca
      241 agccgccggg ctcaaagcgt gtgttagccg aatgggccaa cgcaagctcg atcgaagcta
      301 tctcatgggg gtgcctagat gtcctccagc cgctgctcac ctgcgaggtg ccaagtgtcc
      361 ccacggcgtc tgaacagcat ggaatcaatg tgcccccaaa gccctccacg caggtgcatg
      421 acgcgtacga tcgcctcctg gccatccttg ctgaccccga cccgcgagaa tgtgaaaaga
      481 ccggccgcgc caggatgcct ttgatagaat ccctcccatc ctccgtcgaa cgcattcagc
      541 tctgcaagtg ggagtagccg gtagggtccg atgccgcgaa aacgcgcctc gtaggaacct
      601 cggcttgtct ttgtcgagta gtccgcgagc gtcgtggtgt ccatagccat gccggaatcg
      661 ggttctatag ccctcaacgt cgtggggctt tccgattctg gaatggctag aatcacgacc
      721 tgctgaatgg gctcgaactc cgcctcggac atcggcgcgc cggcgtaggg ctcacgttca
      781 aggattactc gaatcgccgc ggagatgacg tcatactcga tctgtgtgac acgctgagcg
      841 tccgtgcagc cgaccgcagc tccaatgaga caactcgcgc tcagcgtcca agcggaaatt
      901 ccacgaaagg aaacgcacac ggaaccctgc cctgcgggag ttggcctttt cggctaacgt
      961 tcaagcttgc cgacgtggcg cgccgcgccg cacgtgccgc gtacggacca ggcgcgacga
     1021 cgccgggcag agttgctgtg gtccgtccac gtttctcccg gcaaggcgcg ccgcgccgta
     1081 cgcggagcgt gcggaccagg cgcgacatgt ccgccggccc acccttgaag gtaagtcccg
     1141 ccgcctcccg gcggcagacc ggcgtaagcc gttcagcggc aagcatttgt tagccgctgc
     1201 cgcacagcgc cctgaggtcg ccactcgcgt ccggaagcgc gttaccgcat ggagatcgtg
     1261 cgcttgacca gatactctac atcgtgctga tcacgctgca atagccacac atggcctcgc
     1321 gagaagctga ccaaccggct ccggggcgga atttcaaact ctccaaccgc ttcgcccgtg
     1381 ctatatcgaa atacccgcgc aactgagcgt tcgctaccct gaccttcgtg cgtgatcgcc
     1441 acgagcgagt cccctgatac gaaaatgcct gcaactccat gtgatcgctc gggaacatgc
     1501 atcgctgccc ggattgaact ccggacctca tccatgggga tcccgcgacc ttggtagtac
     1561 gcggcaaaac tgtcgattcg agcgctcacc acttcggcag gtatgggtcg ggactcggag
     1621 ctgattgcat gtctcacatt tgatgattcg tccgtggtgc tacggatttc gaggtatagc
     1681 gagtcggagc cgcggtcgag cttcagaatg acttcgcggc caggatcggc aatgatgatt
     1741 ggatcttcgc gaaacggctg cacgaatgtc aacatcccgt ccgcgatgcg tgcgcgaccc
     1801 gttccgccac cggcggggat gctgacaagc ggagcagctg aatctcccac gtaccagcga
     1861 actgagtcct ggtcacgtgc aactccaatg ggtgtgccgt caggcgtaat gcccattgga
     1921 tcaatccgcg cgaacttggc cgccaacgtc gggaagagtg aggctaccag cgccgttcct
     1981 cgaaactcca tatcggcccc gaagcgcacc aatttccccg cccgggcgtc gccgaccagg
     2041 atatcgtccg ggctcgtttg aacgacgttc ggcatgacga gatcagctgg accggcacct
     2101 gctcctccca gcgtggcgac tcgatgtccc acggagtcaa acacagccac gaccgggctt
     2161 ccgaactgca gtacgaaaac cttgccatca tccgactcat gaaccgcggc tacttggtgc
     2221 agcacttggc cggaatcgcc gtcgagtgtc ccgatctgta cggtcggtgc tgataactgc
     2281 caactcgcat cgaccggcgc ctcactgcat gacggcaggc caagaagcaa cacggccaag
     2341 ctgaggcgac gtgacgacgc tgttctcatc atttcaccca gcgctgtgcg gttgcggcta
     2401 acgttcgagc ttgccgacgt gccacgcaga gctcgtggtg agcgtcagcg atccacgggc
     2461 gcgcagtggc atgtccgcgc gtccatgctt caaggtacgt cctcgcgcaa ccgcgcggca
     2521 gggacgcgca gcggccctgc ggcaagcatg tgttagctga cgtacgccgc tcgccggatc
     2581 cttcaccgat cctcacgccc ggcccatgcc attcctggct ccgcccggaa cgaacggcac
     2641 gccgcaagct cccccacgcg atgcaccggc tcccttcacg cctgagctgc caggtcatgg
     2701 caaaacgagc acgcgcaata cgccgcggag ctcggtcacc ccaagcccct ggcgggcaga
     2761 tggacaatcg gcgcgttcat cacat
//
LOCUS       DASUOM010000330         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_871013,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000330 DASUOM010000000
VERSION     DASUOM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871013"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..2770)
                     /gene="infB"
                     /locus_tag="VFY27_07010"
     CDS             complement(254..2770)
                     /gene="infB"
                     /locus_tag="VFY27_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5765300.1"
                     /translation="MADVTVEQFANVLKVPVERLLSQLDEAGIKVSGSDDYISEDAKL
                     ELLTHLRRSHGQQDSSGVSGAPRRITLKRKSQSELRLAGAQGRSRTVNVEVRRQRTYI
                     KRDVLEQQAQKEQEELDRQRKEEQDRIEAEKREQERLKAEKEEQARLEEESKRKHDEA
                     EQQAQNEAREAAQRAAAAAADEKARLEKAEQDARDRRQKEKVKGKQPETRYGRKELHV
                     AGDKSGRRKRKQPSRRRVAIASDGQHGFERPTAPVVREVEIPESIAVSELAQRMAVKA
                     SEVIKILFNMGAMVTINQVIDQDTATLVVEELGHTAKQVSGADRDESLLADEPQSEGE
                     QVQRAPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYHVETEKGGVTFLD
                     TPGHAAFTAMRARGAQATDIVILVVAADDGVKPQTIEAIEHARAAEVPIVVAINKIDK
                     EGADIDRVKNELSQREVISEDWGGDTLFVNVSAYTGEGIEALLDAILLQAEVMDLKAV
                     ADGPAQGIVIESSLEKGRGAVATLLVQSGTLKQGDMIIAGEEYGRARNMFDETQQSIS
                     TAGPSTPVVVLGLSKTPSAGDDFLVVKNERKAREVAEFRQSKSRETKLAQQQASRLED
                     VFSQMTEAEGAVVPLLIKSDVHGSAEALRDALTKLSNDEVKVKVISSGVGGITESDAT
                     LAAASNAVIIGFNVRADNAARAAIKESGVDIRYYSIIYEAIDDVKSAITGLLRPEIRE
                     TIVGLAEVREVFASPKLGNIAGCIVLEGYVKRANPIRVLRDNVVIYEGELESLRRFKD
                     DVNEVRAGTECGIGVRNYNDVKVGDQIECYERVEVARTLE"
BASE COUNT          445 a          951 c          794 g          638 t
ORIGIN      
        1 tcatccggac tgagaaggct gtagtagacc ttcgccacac ccaagtcgcg cgacaggtcc
       61 accgctgtca acgatacggt cgacaacctc gggtccttgg tttcaaatcg cagcagttcg
      121 ctcaaggtgc gcaatacctg cgcccccagc ctctggctgc gtgaaaactc gcgtggcata
      181 gttttaatcc gtgacgggag gtagcgacca accggacgcg accttaagat cgtaaaggtt
      241 gctgctgggc aggctattca agggtgcggg caacctcgac tcgctcatag cattcgatct
      301 ggtcgccaac cttgacatca ttatagttcc tgacgccgat accgcattcg gtgccggcgc
      361 gaacttcgtt gacgtcgtcc ttgaaccgtc gcagcgactc gagctcacct tcgtaaatca
      421 ccacgttgtc gcgaagcacg cgaatcgggt tggcgcgctt cacgtaacct tcgagaacga
      481 tgcaaccagc gatgttgccg agcttcggcg aagcgaatac ttcccggact tcggccagac
      541 cgacaatcgt ttcccggatc tccggccgca gcagtccggt gatcgccgac ttaacgtcat
      601 cgatggcctc gtaaatgatg ctgtaataac ggatgtcgac gccggattcc ttgatcgcgg
      661 cccgtgcggc attgtccgcc cgcacgttga acccgatgat cacggcgttc gatgcggcag
      721 ccagcgtggc atcggattcg gtgatgccgc cgacgcccga gctgatgacc ttgaccttca
      781 cctcgtcgtt cgacagcttg gtcagcgcgt cccgcaaggc ctcggcgctg ccgtggacgt
      841 cggacttgat cagcaacggg accacggcgc cttcggcttc ggtcatctgg ctgaacacgt
      901 cctcgaggcg cgacgcctgc tgctgggcaa gcttggtctc gcgcgacttg ctctggcgga
      961 actcggcaac ttcccgggct ttacgttcgt tcttgaccac caggaaatcg tcgccggcac
     1021 tgggagtttt cgaaagaccc agcacgacta ccggcgtgga cggacccgca gtgctgatcg
     1081 actgttgcgt ctcgtcaaac atgttgcgcg cgcggccata ttcttcgccg gcgatgatca
     1141 tgtcgccctg cttcagcgta cccgactgaa caagcagcgt ggcaacggcg ccgcgccctt
     1201 tttccaggct cgattcgata acgataccct gggcaggacc gtcggccacc gctttcaggt
     1261 ccatgacctc cgcttgcagc aggatcgcat caagcagcgc ttcaattccc tcgccggtgt
     1321 aggccgacac gttgacgaac agcgtatcgc cgccccaatc ttcggagatg acctcgcgct
     1381 gtgacagctc gttcttcacg cggtctatgt cagcgccttc cttgtcgatc ttgttgatcg
     1441 ccaccacgat cggtacttcg gcggcacgcg cgtgctcgat ggcttcgatt gtctgcggct
     1501 tgacgccatc gtcggcggca accacgagaa tgacgatgtc cgtcgcctgt gcgccacgcg
     1561 cccgcatcgc ggtaaacgcg gcgtgccccg gcgtatcgag aaacgttacg ccgccctttt
     1621 ccgtttcaac gtggtaggcg ccgatatgct gcgtaatccc accggcttct ccggcggcga
     1681 ccttggtgcg acggatgtaa tcgagcagcg acgtcttgcc gtggtcaacg tgacccataa
     1741 tggtgacgac cggtgcccgc tgcacctgtt cgccctcgct ctgcggttcg tcggcaagca
     1801 ggctttcgtc cctgtctgcc ccgctgacct gtttcgcggt gtggcccagc tcctctacga
     1861 ccagcgtcgc agtatcctgg tcgatcacct ggttgatcgt gaccatcgca cccatgttga
     1921 acaggatctt gatgacttcg ctggccttca cggccatgcg ctgcgccagt tccgagacag
     1981 ctatgctttc cgggatttca acctcccgca ccaccggcgc ggtcggcctc tcgaaaccgt
     2041 gctggccgtc cgaggcaatg gcaacgcgtc gcctgctcgg ctgcttgcgc ttcctgcggc
     2101 cgctcttgtc gcccgcaacg tgcagttcct tgcggccgta gcgagtttcc ggctgcttgc
     2161 ctttgacctt ctccttctgg cggcggtccc gggcgtcctg ctctgctttc tcgagccgag
     2221 ctttctcgtc ggctgccgcg gcggcggccc tctgtgcggc ctcacgcgct tcgttctgcg
     2281 cctgctgctc cgcctcgtcg tgcttgcgtt tgctctcttc ctcgagccgc gcctgctcct
     2341 ccttctccgc cttcagccgt tcctgttcgc gcttctcggc ttcgatgcgg tcctgctctt
     2401 ccttgcgctg gcgatccagc tcttcctgtt ccttttgcgc ctgctgctcg agcacgtcgc
     2461 gcttgatata ggtacgctgg cggcgcacct cgacgttcac cgttcgcgag cggccttgcg
     2521 cgcctgccag ccgcagctcc gactgcgact tacgcttcag ggtaatgcgg cgcggcgcgc
     2581 cggatacgcc ggacgaatcc tgctgcccat ggctgcgccg caggtgcgtc agcagctcga
     2641 gcttcgcatc ctcgctaata tagtcatccg acccgctgac cttgattcct gcttcatcga
     2701 gctgcgacaa cagccgctcg acaggaacct tcagtacgtt cgcgaattgt tcgacggtga
     2761 cgtcagccat tctttaactc cacaaatttc atcgatcagg cttgctgctc cgcttcgaac
     2821 cagtgggc
//
LOCUS       DASUOM010000331         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1583971,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000331 DASUOM010000000
VERSION     DASUOM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583971"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFY27_07015"
     CDS             <1..789
                     /locus_tag="VFY27_07015"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5765301.1"
                     /translation="NLYKAAGEIRDRYRGGFPENFEDVVALPGIGRSTAGAIMALACN
                     EHRPILDGNVKRVLARYHAVGGWPGDSAVAATLWQYAEQHTPDVDTAAYTQAIMDLGA
                     TVCTRTHPSCFLCPVSTGCKAYATASVSGFPGRREKKAKPLRQTHMALACCNGDVYLE
                     KRPSSGIWGGLWSLPEFDLEDQLLAWCSVRLNAEPVEVERWSTLRHTFSHYHLDIKPV
                     ALHLDSVPQRIADGASGIWYNYGTAPEFGVAAPVRKLIENLRDK"
     gene            790..1056
                     /locus_tag="VFY27_07020"
     CDS             790..1056
                     /locus_tag="VFY27_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX5765302.1"
                     /translation="MSRIVNCIVLGREAEGLDYAPYPGALGQRIYANVSKEAWQKWLA
                     HQTMLINEYRLTPMEPEARKFLETEMEKFFFGGGSEKPKEFVPE"
     gene            complement(1034..2734)
                     /locus_tag="VFY27_07025"
     CDS             complement(1034..2734)
                     /locus_tag="VFY27_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5765303.1"
                     /translation="MRNPLLSKCVALWLSFVLFAAPAAADVVQAGPLPLQAQSAQRIP
                     GPLRAQSAQPVEAQPVGPDGRPGAQQPGAAGQQQPDPDALLQTPLPGEQPLEAPRLDD
                     QSLLDDLSARTFRFFWETANAEKGLIPDRWPSQPFASIAAIGFALTVYPIGVERGYIT
                     REQARVRTVRTLRFFWHAPQGPEPQGTVGHKGFFYHFLNMETGLRWGTTELSTVDTAL
                     FLGGALFAQSYFNGDHPLEAEIRDLAQKIYERVDWPWAQANAPGITLAWRPERGFSTH
                     AWNGYNEAMIVYLLALGSPTYPLDQGAWNSWTDRYDEVWGSHYQQEFLMFSPGFGHQY
                     THIWIDFREVQDAYMRRRGLDYFKNSRRAARAQHAYAEENPLGWKGYGEGVWGLTASD
                     GPGRALLMYEGEERQFRGYVARGIGYPPTHSGLGQSFDDGTIAPTAAISSIVFTPELS
                     IQGIREMRARYGKHIYSEYGFLDAFNPSFNYDVEVHRGRVIPEFGWVAEDYLGIDQGP
                     IILMIENYRTGFVWNVMRENEHIRRGLRRAGFSGGWLNRTEEGDPPSIQEQVTPAQTP
                     "
BASE COUNT          551 a          936 c          939 g          538 t
ORIGIN      
        1 aatctctaca aggcagctgg cgagattcgc gaccgctaca gaggcggttt cccggaaaac
       61 ttcgaagacg tggtcgcgct gccgggtatc ggacgttcca ccgccggggc gatcatggcg
      121 ctcgcctgca acgagcatcg cccgatactc gacggcaacg tcaagcgcgt actggcccgc
      181 taccacgcag tcggcggctg gccgggcgat tccgccgtgg cggcaaccct gtggcagtac
      241 gcggagcagc acactcccga cgtcgatacc gccgcctata cccaggcgat catggatctc
      301 ggcgctacgg tctgcacgcg cacgcatcca tcctgcttcc tttgccccgt atccactggc
      361 tgcaaagcct acgcgaccgc gagcgtctcc gggtttcccg gcaggcgtga gaaaaaagcg
      421 aagccgctgc ggcagaccca catggctctc gcgtgctgca acggtgacgt gtacctggag
      481 aagcgcccgt cgtcaggcat ctggggcggt ctctggagcc tcccggaatt cgacctggag
      541 gaccagcttc tcgcctggtg cagcgtgcgg ctcaacgcgg aaccggtaga ggtggaacgc
      601 tggtcgacgc ttcggcatac cttcagccac tatcatctcg acatcaagcc ggtcgcgctg
      661 cacctcgact cggtgccgca acgcattgcg gacggcgctt ccggcatctg gtacaactac
      721 gggacggcgc cggagttcgg cgtcgccgca cccgtccgga agcttatcga gaacctgagg
      781 gacaagtaga tgtcacgcat cgtcaactgc atcgtgctcg gcagggaagc ggaaggactg
      841 gactacgccc cgtatcccgg cgccctgggc cagcgcattt acgcgaacgt atcgaaagaa
      901 gcgtggcaga agtggctcgc gcaccagacg atgctcatca acgagtaccg gctgacaccc
      961 atggagccgg aggcgaggaa attcctggag acggagatgg aaaaattctt cttcggcgga
     1021 gggtcggaaa aacctaagga gtttgtgccg gagtaacctg ttcctggatc gacggcgggt
     1081 cgccttcttc tgtccgatta agccagccgc cgctgaatcc cgcccgcctc agcccgcgtc
     1141 gtatgtgctc gttctcgcgc atgacgttcc atacgaagcc ggtgcgatag ttctcgatca
     1201 tcaggatgat cggaccctgg tcgattccca ggtaatcttc ggccacccag ccgaactccg
     1261 gtatcacgcg gccgcggtgc acttccacat cgtagttgaa gctcggattg aaggcatcga
     1321 gaaagccgta ctccgagtag atgtgcttgc cgtagcgggc gcgcatttcg cgaattccct
     1381 ggatcgacag ttccggcgtg aacactatgg acgatatagc ggcggtgggt gcgatggttc
     1441 cgtcgtcgaa cgattgcccc agccccgagt gcgtcggggg atagccgatg ccgcgggcaa
     1501 catagccgcg gaactgccgt tcttctcctt cgtacatcag gagcgcgcga cccggtccat
     1561 cgctcgcggt cagtccccag acgccctcgc cgtagccttt ccagcccagg gggttctcct
     1621 cggcgtaagc gtgctgcgcg cgcgccgcac ggcggctgtt cttgaaataa tcgagcccgc
     1681 gccggcgcat gtaggcatcc tggacctcgc ggaaatcaat ccagatgtgc gtgtactggt
     1741 gtccgaagcc ggggctgaac atcaggaatt cctgctggta atggctgccc catacctcgt
     1801 cgtagcgatc cgtccacgag ttccatgcgc cctggtccag cgggtaggtc ggcgatccca
     1861 gcgcgagcag gtagacgatc atggcctcgt tgtagccgtt ccaggcgtgc gtgctgaagc
     1921 cgcgttcggg gcgccaggcg agcgtaatgc cgggagcgtt cgcttgcgcc cacggccaat
     1981 cgacgcgctc gtagattttt tgcgccaggt cacggatctc cgcttccagc gggtgatcgc
     2041 cattgaaata cgactgcgcg aaaagggcgc cgcccaaaaa cagcgctgta tccaccgtcg
     2101 acaactcggt ggttccccag cgcaggcccg tttccatgtt caggaagtgg tagaaaaagc
     2161 ctttgtgtcc taccgtccct tgaggctccg gcccttgcgg tgcgtgccag aaaaaacgca
     2221 gtgtccgcac cgtccgcacc cgcgcctgct cgcgcgtgat atagccgcgc tcaacgccga
     2281 tcgggtagac ggtcagggcg aaacctatgg ccgcaatgga ggcgaagggc tgcgacggcc
     2341 agcgatccgg aatcagtcct ttttccgcgt tggcagtctc ccagaaaaag cggaaggtgc
     2401 gcgcggatag atcgtcgagc agcgactggt cgtccagccg cggcgcctcg agcggctgct
     2461 cgccgggtag cggcgtttgc agcagggcat cgggatcagg ctgttgttgt ccagcggccc
     2521 caggctgttg cgcgccgggt cgcccgtccg ggccgaccgg ctgcgcttcg accggctggg
     2581 ccgactgtgc ccgcaagggc cctggtatgc gctgcgccga ctgcgcctgc agtggcagtg
     2641 gtcccgcctg tacgacatcc gccgcagcag gagcagcaaa caggacgaac gaaagccaca
     2701 aggcgacgca cttgctgagg agcgggtttc tcaagctgtc gataaccatg cagcggcaat
     2761 acctgcgctg gaaactggtg cttcgggagt ttagccgccc cgcgacagag gcaaagtgat
     2821 ggaatgcgca aaagcgagcc gcattttcca tgatcgattc cgcacccgca accgaccggc
     2881 gatgcgttcg cggttgcaaa cgcggtagcc gctggccgtt attcgcaagg cgtaggagcc
     2941 cgccatgtgg gcgagttcgg tttg
//
LOCUS       DASUOM010000332         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1446430,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000332 DASUOM010000000
VERSION     DASUOM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446430"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFY27_07030"
     CDS             complement(<1..1080)
                     /locus_tag="VFY27_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX5765304.1"
                     /translation="MNRRLLQWAAFLFGVFHIYTNVFASMSELWFSAIHFGGFGALCA
                     LSFHDPDRQEPGRFLTNALRVVLAALSVASAAYLVLFENALYARETEFIWSDYLFSGL
                     AVLLAIELTRRTSGWFMPALIIVSLSYMLFLGRYLAGVFSFPGLSLETVLYRSYFSST
                     GMFGFVANISATYVFMFILFGSFLLKSGAGDFIIRLAHSVAGRFTGGAGMVAVVSSGL
                     MGSVTGSAVANTVSTGVITIPMMKRSGFSPRFSAAVESAASTGGQLMPPVMGAGAFVM
                     ASYTQIPYTHIIAVSVLPALLYFLTVAFFIRGEAIRLGIAVDHVQRGPGMIAIVREGW
                     HFIGPLALLVVLLAVGFTPVRSVSLA"
     gene            complement(1077..2090)
                     /locus_tag="VFY27_07035"
     CDS             complement(1077..2090)
                     /locus_tag="VFY27_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5765305.1"
                     /translation="MWRILYKLRFFAGLVLTILLSCSGELAADRPYVLATATVGGTYY
                     PVGVALATISKSKLYPEHGVSLAAISSAGSLENIKLLRDNQAQFALLQGVFAAWAWNG
                     EGPVREPQAWLRSVSGMWHNVEHFALLADLVQEGTLSDLGALDGQRFVLGERNSGAEH
                     TGAYILESLGIDYRRRMNLAYMGYGATADAIQDGNIVGMNIPAGVPVSAITRALAVLG
                     SRMAVLEVSDDELAKINARYPLWNHYEIAAGTYPNQLSPIRSISHPNVLAVREDVPDE
                     TVYQVTRIMWENLSTLQEIHRSTNDMKLELALKGLGAPLHPGAARFYRERGVDIPPHL
                     APP"
     gene            complement(2258..>2669)
                     /locus_tag="VFY27_07040"
     CDS             complement(2258..>2669)
                     /locus_tag="VFY27_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5765306.1"
                     /translation="HPAGPVTAEELRRIRDEEAQHFVMLKEAMRTLGADPTAQTPDAD
                     VIGVANMGIAHALNDPRTTMSQCLDVLLTVELGDNAGWQLLIALAEELGMDELVEKFQ
                     VALTQEEEHVRRIRSWYEQMVLSQSAGKRVEVTH"
BASE COUNT          548 a          842 c          817 g          462 t
ORIGIN      
        1 cgcgagcgag acggagcgga caggcgtgaa accgacagcg agcaggacaa ccaacaaggc
       61 cagcggccct atgaaatgcc agccttcccg gacgatggcg atcatgccgg ggccacgttg
      121 gacgtggtcc acggcgatcc cgaggcgtat tgcctcgccg cgaatgaaga atgcgacggt
      181 gagaaagtaa agcagagccg gtaacaccga tacggcgatg atgtgcgtat acgggatctg
      241 cgtgtagctc gccatcacaa aggcgccggc tcccatgaca ggtggcatca gctggccccc
      301 ggtcgaagct gcggattcga ccgctgcgga aaatcgcgga ctgaaaccgg agcgcttcat
      361 cattggaatg gtgatgacgc ctgtcgatac ggtgttcgca acggcagagc cggtgacgga
      421 gcccatgagc cccgacgaca caactgccac catccctgca ccaccggtaa accggcccgc
      481 aacgctgtgc gcgagcctga tgatgaagtc gcccgcgccg gatttcagga ggaaggaacc
      541 gaacaggata aacatgaaga cgtaggtcgc cgagatattc gccacgaaac cgaacatgcc
      601 cgtgctgctg aaatagctgc ggtacagcac cgtctcgagg ctcagtcccg ggaaggaaaa
      661 aacccccgcc aggtaacggc ccaggaacag catgtaggag aggctgacga tgatgagcgc
      721 aggcatgaac cagccgctcg tccgccgggt cagctcgatt gccagcagga cggccagccc
      781 ggagaacagg tagtctgacc agatgaattc cgtttcccgc gcgtacagcg cgttctcgaa
      841 caggaccagg tatgcggcac ttgcgaccga cagcgccgcc agcaccacgc gcagcgcgtt
      901 ggtaaggaat ctccccggct cttggcggtc gggatcgtgg aacgacagtg cgcacagcgc
      961 gccgaagcca ccgaagtgga tggcggagaa ccacagctcc gacatggaag cgaagacatt
     1021 ggtgtatatg tggaaaacac cgaagaggaa tgcagcccac tgaagtagcc ttctgttcac
     1081 ggcggcgcca ggtgcggagg tatatcgact ccgcgttcgc gatagaagcg ggctgctccc
     1141 ggatgcagcg gcgcacccag gcccttcagc gcgagctcga gcttcatgtc attggtcgag
     1201 cggtggatct cctgcagggt cgacaggttt tcccacatga tcctggtgac ctggtacacc
     1261 gtctcatccg gcacgtcctc gcgcaccgcg agtacattcg ggtgcgatat cgaccggatc
     1321 ggggaaagct ggttcggata tgtgccggcg gcaatctcgt agtggttcca gagtgggtag
     1381 cgcgcgttga tcttcgcgag ttcatcgtcg ctcacctcaa gtacggccat gcggctcccc
     1441 agtaccgcca atgccctggt aatggcgctc accggtaccc ctgcgggaat gttcatgccg
     1501 acgatgttgc cgtcctgtat cgcatcggcg gtcgcgccat accccatgta ggcaaggttc
     1561 atcctgcgcc ggtagtcgat acccaggctt tccaggatgt acgcaccggt atgttctgcg
     1621 ccggagttcc gctcgccgag cacgaaacgc tggccgtcga gcgccccgag gtccgacagg
     1681 gtgccctcct gcaccaggtc cgcgagcagt gcgaaatgct cgacgttgtg ccacattccg
     1741 ctgacgctgc gcagccacgc ctgcggctcc cgcacagggc cctccccgtt ccatgcccag
     1801 gccgcgaata ccccctgcag cagcgcgaac tgcgcctggt tgtccctcag taacttgatg
     1861 ttctcgagcg aacccgccga actgatcgcc gcaagcgaaa cgccgtgttc cgggtacaac
     1921 ttcgacttgc tgatggtcgc aagtgcaacg cccaccgggt aataagtccc cccgaccgtc
     1981 gcggtcgcga ggacgtaagg cctgtccgcg gcgagctcgc cggaacagga aaggaggata
     2041 gtgaggacga gaccggcgaa gaatcggagc ttgtacaaaa tgcgccacat ggcgccgatg
     2101 ctagcctaac gattcatggc ggtcactccg cggatggtaa gcgtcgcctg atgcccgagc
     2161 ctgtaggagc ccgccatgcg ggcgagttcg ctgtgctgaa caacctcggt gcactcgccc
     2221 gcgtagcggg ctcctacagg tgccagttga tcggcgttca gtgcgttact tcgactcgct
     2281 tgccggcaga ctgcgacagc accatctgct cataccagga acggatgcgt cgcacatgct
     2341 cttcttcctg cgtcagcgcc acctggaatt tttcgaccag ctcgtccatc cccagctcct
     2401 cggccagtgc gatcaggagt tgccagccgg cgttgtcgcc aagctccacc gtcagcaaca
     2461 cgtccaggca ctgcgacatg gtggtacgcg gatcgttgag cgcatgtgcg atacccatgt
     2521 tagccacgcc gatcacatct gcatccggag tctgcgctgt cggatccgcg cccagcgtac
     2581 gcatcgcctc cttcagcatc acgaagtgct gggcttcctc gtcgcggatg cgtcgaagct
     2641 cttccgcagt aaccggcccg gcaggatgc
//
LOCUS       DASUOM010000333         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_511566,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000333 DASUOM010000000
VERSION     DASUOM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511566"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFY27_07045"
     CDS             complement(<1..718)
                     /locus_tag="VFY27_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5765307.1"
                     /translation="MEQLLDPTILLGLLTLIVLEVVLGIDNLIFIAILSDKLPEHQRD
                     KARVVGLSLAMFMRLALLASISWLVSLTEPLFSVLGQSFSGRDLILLGGGLFLLFKAT
                     TELHERLEGGSHHSDGPKAHAAFGVVLAQIVALDAVFSLDSVITAVGMVDELWVMMAA
                     VIIAIILMIIASKPLTRFVNAHPTVVVLCLAFLLMIGFSLIAEGFGMHIPKGYLYAAI
                     GFSVLIEFLNQTARRNRAKRA"
     gene            complement(852..1199)
                     /locus_tag="VFY27_07050"
     CDS             complement(852..1199)
                     /locus_tag="VFY27_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765308.1"
                     /translation="MCRREAARLAAPSSGYFLDQFTNASRAVDWQAHNVIVELFDQLH
                     RGAIQDPDWFVMGAGTGGTAALVDRMSVIPDAASYGAARWLSAKLDRPVGARERDLAA
                     TRHEPRPERNGVT"
     gene            1403..2290
                     /gene="htpX"
                     /locus_tag="VFY27_07055"
     CDS             1403..2290
                     /gene="htpX"
                     /locus_tag="VFY27_07055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX5765309.1"
                     /translation="MFKRVVLFIATNLAVILVLSVVLRLLGVDRILDESGTGINYESL
                     LILSLVIGFGGSFISLAISKWMAKRTTGARVITNPSNNTEAWLIGTVQRLSQQAGIDM
                     PEVAIYDAPDMNAFATGARKNSALVAVSSGLLQGMQREEVEAVLAHEISHVANGDMVT
                     LALVQGVVNTFVIFLSRIVGHIVDRVVLKNDRGYGIGYFVSVLAAQLVLGILASIIVM
                     WVSRHREFRADAGSARLNGKKPMIDALARLDRTSRPAALPENLEAFGISGRQAMGFKR
                     LLMSHPPIVERIDALQRLP"
     gene            complement(2485..2643)
                     /locus_tag="VFY27_07060"
     CDS             complement(2485..2643)
                     /locus_tag="VFY27_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765310.1"
                     /translation="MNTPKPDTVRTALSEIRERCRELLDEPEATSELELEDTDDPNRG
                     SGPYHRLR"
BASE COUNT          540 a          876 c          866 g          572 t
ORIGIN      
        1 tcgctcgctt cgctcggtta cgcctcgcgg tctggttcag gaactcgatg aggacagaga
       61 agccgatagc agcatacagg taacccttcg ggatatgcat gccgaagcct tccgcgatca
      121 ggctgaaccc gatcatcagc aggaacgcga gacacagcac gacgacggtg gggtgcgcgt
      181 tcacgaatcg cgtcagcggt ttgctcgcaa tgatcatcag gatgatcgcg atgatgactg
      241 ccgccatcat tacccacagc tcgtccacca tcccgacagc ggtgattacg gaatccagcg
      301 agaacacggc atcgagagca acgatctgcg ccagcacgac tccgaaggcg gcatgggctt
      361 tgggcccgtc ggagtgatga gagccccctt ccagtcgctc atgcagttcc gtcgtcgcct
      421 tgaacagcag gaaaagtccg ccacccagca ggatcaagtc tcttccggag aagctctggc
      481 caagtaccga gaacagcggt tcggtaaggg ataccagcca ggaaatgctg gcaagcagcg
      541 cgagccgcat gaacattgcg agactcagtc cgacaacccg cgccttgtcg cgctggtgct
      601 caggcaactt gtccgacagg atcgcgataa agatcaggtt gtctatcccg aggacaacct
      661 cgaggacgat cagggtcagc aggccgagca ggatggtcgg gtcgagaagc tgttcgatca
      721 tgggggccag gacgtggggg ttatgaggga gtatgccacg agccgggagc agcccctaaa
      781 ttcggtatgg cttttgccgc tcccgcgccc gcccgggcct gccgcacggc aaattgttgc
      841 tgcagggccg ctcaggttac cccgttgcgc tcagggcgcg gttcgtgacg ggtggccgcc
      901 agatcccgct cgcgtgcacc gaccggccgg tcgagcttcg ccgacagcca ccgggccgcg
      961 ccgtatgatg ccgcgtccgg tatgacactc atcctgtcca cgagcgcagc cgtaccgccg
     1021 gtgcctgcgc ccatcacgaa ccagtccgga tcctgtatcg ctccccggtg cagctggtcg
     1081 aacagttcca cgatgacgtt gtgtgcctgc cagtcaaccg cccgcgaagc gttcgtgaac
     1141 tggtctagaa agtacccgct gctgggagcc gccagacgag ccgcttcacg cctgcacatt
     1201 cggccttgat ccaggtcaac ccctcctgct actagctctt aagagtacgc tacaagggct
     1261 tgatattcag ctctttctat attcggcgat cccgtctccg attcctcccc ggattcccgc
     1321 gacggagata acggcagccg ctacttgaaa gccctttctt tcgccacaaa atcaggcgcc
     1381 tgtaacctgg aaggagtttc ggatgttcaa gcgcgtcgtc ctgttcatcg ccaccaacct
     1441 ggccgtgatc ctggtgttga gtgtcgtgtt gcgcctgctt ggcgtggacc ggatcctcga
     1501 cgaatccggg accggcatca attacgaatc gctgctgatc ctgtcgctgg tgatcggctt
     1561 cggaggctca ttcatttcac tggccatctc gaagtggatg gcgaagcgca ccacgggcgc
     1621 ccgggtcatc accaacccct ccaataacac cgaagcgtgg ctaatcggga cggtgcagcg
     1681 gctttcgcag caggcaggca tcgacatgcc ggaggtcgcg atctacgacg cacccgacat
     1741 gaacgcgttc gcgaccggtg cgcggaagaa cagtgcgctg gtcgcggtta gcagcgggct
     1801 gctccaggga atgcagcggg aagaagtcga ggcggtgctc gctcacgaga tctctcacgt
     1861 cgccaacggg gacatggtga cgctcgccct cgtgcagggc gtggtgaaca cgttcgtcat
     1921 cttcctttcc cgaatcgtcg gccacatcgt tgaccgcgtc gtattgaaga acgatcgagg
     1981 ctacggcatc gggtattttg tttcggtact cgcagcgcag ctggtgctgg gcattctcgc
     2041 gagcattatc gtgatgtggg tatcgcgcca ccgggaattc cgcgcggatg cgggctcggc
     2101 tcgcctgaac ggcaagaagc cgatgatcga cgcgctggca cgtctcgatc gaacctcgcg
     2161 tcccgctgcg ctccccgaga acctggaggc gtttggcatt tcggggcggc aggcaatggg
     2221 cttcaagcgg ctgcttatga gccatccgcc gattgtggaa cgcatagacg cgctccagag
     2281 gctaccgtag ccgcttgatc cggcgccgtg gcgttacccg ttgtcgcgtg cgggtcttgc
     2341 cctccccgga ttgacctgtt ttcgacatct ctgctgaggg tttatcgctg tcgggcgatg
     2401 ccgctggcgg ccatgccatc ggccggccgc tccagaacgg gccgccacgg attccggacc
     2461 agcaacggct ccagcgtccg gtgttcatct cagccggtga tacggaccgc tccccctgtt
     2521 gggatcatcc gtgtcctcga gctcgagttc ggaggttgcc tctggctcgt cgagcagttc
     2581 ccggcaacgt tcgcggatct ccgaaagcgc cgtcctgact gtgtcgggct tgggagtgtt
     2641 catacacttc ttctatttcc ttgtgattga tgggcgaagt ctaagcgtga ccggttgatg
     2701 ccgaatgtga cgccgctcac aatttggcat cggcgctgaa gagcatctcc tgcaaactca
     2761 ggtgcttatg taacatcttc gagagtaccg cttaagaacc ggcggggtcg tcaggacatt
     2821 taaaagcgga ccaaggccga accccaggtg aatc
//
LOCUS       DASUOM010000334         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_272900,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000334 DASUOM010000000
VERSION     DASUOM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272900"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFY27_07065"
     CDS             complement(<1..754)
                     /locus_tag="VFY27_07065"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5765311.1"
                     /translation="MELATMAIFSGNAHPKLAHEIAKVLHVPLAKAVVGRFSDGEISV
                     EIMENIRGRETFIIQSTCPPTAENLMELLVMVDACRRASASRITAVIPYFGFARQDRR
                     PRASRVPITAKLVANLIGTAGVDRVLTVDLHADQIQGFFDIAVDNVYASPVLLGDVWK
                     QKYANMVVVSPDVGGVVRARALAKRLDDADLVIIDKRRSKANQSQVMNIIGDVKGKTC
                     IIIDDMVDTAGTLCQAAGALKKAGAEKVVAYIT"
     gene            complement(813..887)
                     /locus_tag="VFY27_07070"
     tRNA            complement(813..887)
                     /locus_tag="VFY27_07070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gln,seq:ttg)
     gene            complement(911..1522)
                     /gene="lolB"
                     /locus_tag="VFY27_07075"
     CDS             complement(911..1522)
                     /gene="lolB"
                     /locus_tag="VFY27_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEX5765312.1"
                     /translation="MAETARARMRRLAMALLLLAVAGCATQRGAGLPEFDDWDSRRDA
                     LSSLSEWEFHGRIGVSAGDDGFNGKLRWWQHDDVFVASVSGPLGVGTVKIQGDERQVS
                     VTDEDGVVTEMQDAEAELYRRYGWTIPVTSLRYWALGIPDPSSPAETAFNGEGQLARL
                     EQRNWAVRIDAYREGGGQQMPRRMVATHRDTKVRLVIDNWIFH"
     gene            complement(1533..>2848)
                     /locus_tag="VFY27_07080"
     CDS             complement(1533..>2848)
                     /locus_tag="VFY27_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765313.1"
                     /translation="RSFGRLLDRGKEPVDERLVALIPFLSEEDPEDADWLMRQLAKPY
                     KDSASANYAAGVLALQAGDTEEARERAQKAIELKKEWVQPHLLYARSLLLAGDEKAAI
                     DYTARMVGDNPDPDPEARLELAIMYLAAERDEDALSQVNQVLLEQPARTDALRLMAII
                     NFRQENLDAAWSDFQDLLASGRHTMDALYYLGRIADRRQEYDQAVALYSQVIAGQNAV
                     PAQRRASGIMAEEGEPEKAITHLEEFAETHPNFAIDMMQARAQLLASLDRYPEALDLY
                     DRVVEYRPDSESVALGKAELLLRMDRVDEAIDEYRDAVKRWPDSAMTLNALGYTLADR
                     TTRYREAARLIRKALDKEPESAAIIDSWGWVLYRQGKYEEALVELNRAYESLKDPEVA
                     AHIVEVLWKLERLDEAQRVLEDAETIFPENEILENVRERVFPESR"
BASE COUNT          501 a          919 c          856 g          572 t
ORIGIN      
        1 gcgtgatgta cgccaccacc ttttcggcgc cggccttttt cagggcgccg gcagcctggc
       61 aaagcgtgcc ggcagtatcc accatgtcgt cgatgatgat gcaggtcttt cccttcacgt
      121 cgccgatgat gttcatcacc tgggactggt tcgctttcga acggcgcttg tcgatgatca
      181 ccaggtctgc atcgtccagc cgcttggcca gggcgcgtgc ccggacgacg ccaccgacat
      241 cgggcgacac gacaaccatg ttcgcgtact tctgcttcca gacgtcgccc aacagcaccg
      301 gcgaggcgta tacgttgtcc acggcgatgt cgaagaaacc ctggatctgg tccgcatgca
      361 ggtccacagt gaggacacgg tcgacgccgg ctgtaccgat gagattcgcg acgagctttg
      421 cggtaatggg gactcgcgaa gcccgcgggc gtcggtcctg tcgcgcgaag ccgaagtagg
      481 gaatcacggc cgtgatgcgc gaagcagacg ccctgcggca cgcgtccacc atcaccagca
      541 gttccatcag gttctcggcg gtcggcgggc aggtggactg gataatgaag gtttcccggc
      601 cgcgaatgtt ctccattatc tcgacgctga tttcgccgtc gctgaagcgt cccaccacgg
      661 cctttgcaag cggcacgtgc aggaccttgg caatctcgtg cgcaagcttc gggtgcgcgt
      721 tgccggaaaa gatagccatc gttgccagtt ccacggctat gtttctcctt cgtttgtaca
      781 caagtagcga gttacccgaa gctacggggg cgtggctggg gtggcaggat tcgaacctgc
      841 gcatgacggg atcaaaaccc gttgccttac cacttggcta caccccaatc gcgcgaattg
      901 tgcggggcgg ctaatgaaaa atccagttgt cgatgaccag ccggaccttg gtgtcgcgat
      961 gcgtagccac catgcgccgc ggcatctgct ggccgccgcc ctcgcggtat gcatcgatgc
     1021 gtacggccca gtttcgctgt tcgagccggg cgagctgccc ttcaccgttg aaagcagttt
     1081 cggcgggcga tgagggatcc ggaattccca gcgcccagta gcgaaggctc gtcaccggaa
     1141 tggtccagcc gtagcgccgg taaagctccg cctccgcgtc ctgcatctcc gttactacac
     1201 cgtcctcgtc ggtcaccgaa acctggcgct cgtcaccctg tatcttgacg gtaccgacgc
     1261 cgagcgggcc gctgaccgag gcgacaaaca catcgtcgtg ctgccaccag cgcagttttc
     1321 cgttgaaccc gtcatctccc gcgctgacac cgatgcgtcc gtgaaactcc cattccgaaa
     1381 gggaagaaag tgcatcgcgc cgagagtccc agtcgtcgaa ctccggcagc ccggcgcctc
     1441 gctgggtggc gcagccggct accgcgagga gcagcaaggc cattgcaagg cgacgcatcc
     1501 tggcgcgcgc cgtctctgcc atgtgcgatt tatcagcggc tttcggggaa cacacgctca
     1561 cgcacgttct cgaggatttc gttctccggg aagatggttt cggcgtcctc gagaacccgc
     1621 tgcgcttcgt caaggcgttc gagtttccac aacacctcca cgatgtgcgc ggcaacttcc
     1681 ggatctttca gcgattcgta ggcgcggttc agttctacca gggcttcctc gtacttgccc
     1741 tggcgataca gaacccaacc ccagctgtcg ataatggcgg cgctttccgg ttccttgtcc
     1801 agcgccttcc ggatcagccg cgccgcctcc cggtacctgg tcgtgcgatc ggcgagcgtg
     1861 tagcccagcg cattgagcgt catggcgctg tcgggccagc gcttgaccgc atcgcgatat
     1921 tcgtcaatcg cctcgtccac cctgtccatg cgcagcagga gttctgcctt gccgagggcg
     1981 acgctctcgc tgtcggggcg gtactcgaca acccggtcgt acaaatccag cgcttccggg
     2041 taacgatcga gcgatgccag caactgcgca cgcgcctgca tcatgtcgat ggcgaagttc
     2101 ggatgggtct cggcgaactc ctcgagatgc gttatcgcct tctcgggttc accttcctcg
     2161 gccatgatcc cgctggcacg gcgctgcgcg ggcactgcat tctggccggc aattacctgg
     2221 gaatagagcg ccactgcctg gtcatactcc tgcctgcggt cagcaatgcg gccgaggtaa
     2281 taaagcgcat ccatggtgtg acggccgctg gcgagcaggt cctggaagtc ggaccatgcc
     2341 gcgtcgaggt tttcctggcg gaagttgatg attgccatca aacgtaaagc gtcggtccgg
     2401 gccggttgct ccagcagaac ctggttcacc tggctcaggg catcctcgtc ccgctccgcc
     2461 gccaggtaca tgatcgccag ttcgagccgc gcctcagggt cagggtcggg gttgtcaccc
     2521 accatgcgag cggtgtaatc gatagccgct ttctcatccc cggcgagtag caaagatcgt
     2581 gcatacaaaa gatgcggctg tacccactcc ttcttgagct cgatcgcctt ttgcgcacgc
     2641 tcccttgcct cctcggtatc gccggcctgc agcgcgagta cgccggcggc ataatttgcg
     2701 gaagccgaat ccttgtacgg cttggcaagc tgccgcatga gccagtcggc atcctcggga
     2761 tcttcctcgg agaggaacgg gatgagggcg acgaggcgct cgtcaaccgg ctccttaccg
     2821 cgatcgagca ggcgcccgaa gctacgcc
//
LOCUS       DASUOM010000335         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1119959,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000335 DASUOM010000000
VERSION     DASUOM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119959"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="rarD"
                     /locus_tag="VFY27_07085"
     CDS             complement(<1..741)
                     /gene="rarD"
                     /locus_tag="VFY27_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX5765314.1"
                     /translation="MTWAPTAVSETPNRDPAISRDEHSPELRTRDGVITALLAYVTWG
                     FLPIYFKWIEEVPALEVLAHRIVWAVPFGAVIIVARRQWPEVRRAVAHRRTLALLGLA
                     ALFIAVNWLVYITAVQRDQIFQASLGYYINPLMYVVIGVLVLREKLRKPQTAAVLLAA
                     AGVAVLTFSGGEFPVISLTLAVSFTIYGVIRARVVVGGMPGLFIETLLLFPMSLAYLL
                     WLMQAGEAGFALDNPGMAGALMLAGPLTV"
     gene            complement(870..1364)
                     /locus_tag="VFY27_07090"
     CDS             complement(870..1364)
                     /locus_tag="VFY27_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765315.1"
                     /translation="MRCKRTAGVGLFAGVLLLAACGEEIAEQQVVPDEPLQKPACELI
                     EQVDLQQIFVNTLVPDTSSATSNRECTWMDEATGKPFVQYSLHPYREDLEAQVHVIEQ
                     ERGDSINARFVQGIGDAAVWTDVGLFANRNGRTLQVQPLQDDQNEAPYQELARLLLTR
                     LEDS"
     gene            complement(1451..1960)
                     /locus_tag="VFY27_07095"
     CDS             complement(1451..1960)
                     /locus_tag="VFY27_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5765316.1"
                     /translation="MAISVFDQTVSAMSRMLLNLDAIVSKAEIYAEEKKIAPEVLLQG
                     RLYPDMLPFVSQVRIATDTAKGAASRLTGCEMPKWADDETTFEEIHSRIRKALNYLAD
                     FKPEQFENSETRDVELKLPNRTLQFTGRDYVLGFVLPNFYFHVTTAYNILRHNGLQIG
                     KLDFMGAPE"
     gene            complement(2230..>2439)
                     /locus_tag="VFY27_07100"
     CDS             complement(2230..>2439)
                     /locus_tag="VFY27_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX5765317.1"
                     /translation="FWEALRGCRQLLAAEHKVPPYVIFHDATLMQIIDRRPRDEHELL
                     GISGVGQAKLQRYGEAFLSVIREYV"
BASE COUNT          464 a          758 c          748 g          469 t
ORIGIN      
        1 gacggtgagg ggacccgcca gcatcagagc gccggccatt cccggattgt ccagtgcgaa
       61 cccggcctcg cccgcctgca tgagccacag caggtaggcg agcgacatcg ggaacaggag
      121 caacgtctcg atgaacaggc ctggcatgcc cccgacgact accctggcgc ggatgactcc
      181 gtaaatcgtg aacgagacgg cgagcgtcag tgagatgacc gggaattcgc cgccgctgaa
      241 cgtcagcacc gcaacgcccg cggcggcgag cagcacggcg gccgtctgcg gcttgcgcag
      301 tttctcgcgc agcacaagta cgccgatgac cacgtacatg agcgggttga tgtaatagcc
      361 gaggctcgcc tggaatatct ggtcgcgttg gacggcggtg atatagacga gccaattcac
      421 ggcgatgaac aacgcggcga gtcccagcag tgcgagggta cggcgatgcg cgacggcgcg
      481 gcgaacttcc ggccactggc gccgggccac gataatgacg gcgccgaagg gcaccgccca
      541 cacgatccgg tgtgcgagca cctcgagcgc cggcacctcc tcgatccatt tgaagtagat
      601 cggcaggaat ccccatgtga catacgccag cagcgcggta atgacgccgt cacgcgtgcg
      661 aagctcgggg ctatgctcat ccctggaaat ggcaggatct ctgttgggag tttcggaaac
      721 cgctgtagga gcccacgtca gactacggcg ctacgagcat tatctggcag tcgttttcgg
      781 gcggcgaccg cggacgagtg cgacaaggcc cagcgaactc gcccgcgcgg cgagctccta
      841 caacctctcg gatcagtcgg tagagactcc tagctgtctt ccaggcgggt caataacagg
      901 cgtgccagtt cctggtaggg agcctcgttc tggtcgtcct gcaacggttg tacctgcagc
      961 gtccggccgt tgcggttggc gaacagcccg acgtcggtcc atacggcggc atcgccgatg
     1021 ccttgcacga agcgtgcatt gatgctgtcg ccgcgctcct gctcgattac atggacctgc
     1081 gcctcgagat cctcccggta ggggtgaagc gagtactgga cgaaaggctt tcccgtcgct
     1141 tcatccatcc acgtgcattc ccggttggac gtggcggacg aagtgtcggg aacgagggtg
     1201 ttgacgaaga tctgttgcag gtccacttgc tcgatcagct cgcaggccgg tttctgcagc
     1261 ggctcgtccg gaaccacttg ctgctcggct atctcttcgc cacaagcagc taaaagaagg
     1321 acaccggcga agagcccgac tccggccgtg cgtttacatc tcattcctca ctcccatgcc
     1381 tcaacgtgcg aatcagccta cgcttacgat tgaccaaaat ccagatcgga tcggcgcgca
     1441 accggtgtcg ttattccggc gcgcccatga agtcgagctt gccgatctgc aagccgttgt
     1501 gccgcaggat gttgtacgcg gttgtaacat ggaagtagaa attcggtaat acgaagccga
     1561 ggacgtagtc gcggccggta aactggagcg tccggttggg caacttcagt tcgacgtcgc
     1621 gcgtctcgct gttctcgaac tgttccggct tgaagtccgc aagatagttc agggccttgc
     1681 gaatgcgcga gtgtatctcc tcgaacgtgg tctcgtcatc cgcccacttg ggcatctcgc
     1741 agcctgtcag tcgcgaggcc gcgcccttcg ctgtatccgt ggcgattctg acctgcgaca
     1801 cgaatggaag catgtcggga tagagccgtc cctggagcag tacctccggg gcgattttct
     1861 tttcttcggc gtatatctcg gccttggaca cgatcgcatc caggttcagc aacatccgtg
     1921 acatcgcgga tacggtctgg tcgaaaacgg aaatagccat gtgactcctc agcgtttgca
     1981 gatttgaaaa gaactggcgc acggccacag tcagcgcaca tccgcgctgg ccgcgaccac
     2041 ctctttcttg cagtcgggct caagtcgccc gtgtcaggct tcaggctgcg agcttgcccg
     2101 tcgatttccc gacgtgagtc gcgatcgcat ccaggagcgc ggggacaggg ctatctccgg
     2161 gtgtactctc cgaactcgcc ttgaactgcc aggcccgcgc ggcctgtatt gtacatcgca
     2221 cccgtcccgt cagacatact cccggatcac cgagagaaaa gcctcgccgt agcgctgcag
     2281 cttggcctgc cccaccccgc tgatccccag cagttcgtgc tcgtctctcg ggcggcggtc
     2341 gattatctgc atgagcgtcg cgtcgtggaa tatgacgtag ggcggcacct tgtgttcggc
     2401 cgcaagcagc tgccggcagc cgcggagcgc ctcccagaa
//
LOCUS       DASUOM010000336         5513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_530543,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000336 DASUOM010000000
VERSION     DASUOM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530543"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFY27_07105"
     CDS             complement(<1..467)
                     /locus_tag="VFY27_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765318.1"
                     /translation="MIRLLALTLLALECANAEPSSTETHQGIAPDIREDSTNLKLQRG
                     NYVVVPIPISNPTLDTGLIAGAAYFWPQTAEQAARQPASVTGAAAMYTSNDSRVLVLA
                     QQSYWAEDRWRLTGAIGAADLRLTLLTGDGAAGEGRADWHIDGGFLAARLARK"
     gene            complement(479..895)
                     /locus_tag="VFY27_07110"
     CDS             complement(479..895)
                     /locus_tag="VFY27_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765319.1"
                     /translation="MNRILAVALVILAVSTSRADDISGSERLLCSTSRIMACFETGDC
                     AELLAEELDVPQFVVVDTRKKRLTTTKASGENRSTAIGEINRADGLLFFQGIEGGRAF
                     SFVVHEHTGHLTVAVSRDGLSVSVFGSCTDVTDADI"
     gene            complement(1040..2473)
                     /locus_tag="VFY27_07115"
     CDS             complement(1040..2473)
                     /locus_tag="VFY27_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5765320.1"
                     /translation="MSHVTILWSAVATGALLLAIMYGAVWALDRKAYAYLAFAFEALA
                     IVGIVIFELGMMYSTTAAEWGEWLRWFQIPIFLRTVALLAFIRFYFKTGRSWLMWTII
                     TSRAIILIAGFVSDPNFNFASIDSIDRVAFLGEQVTIVGQAVVSPYQWVATASTILVL
                     TFVGDASFTLWRRGTYEARRKVIVIGGAVFLSLAIGIVLTQLMIYGNVHVPALLSPPS
                     MIVLAAMTFELGRDALRTRQKVEELQRELAHAGRVSALGTLSSSLTHELSQPMSAILI
                     NTRLGAQLLKGPNPDLEELRQILDDIRRDNQRAIEVIDHLRSLLKRRSLDFTPVSVER
                     LLEDTSALLKSDAIARNVRLECASDAGTGMVHGDVVHLSQVLINLIINAMDAVADLPD
                     GRRHVSVRACTIDGSWVELAISDSGRGIPPDSLGRIFEPFYTTKAAGMGMGLSISRII
                     VEAHAGRIWAENGGYGTTLRVQLPLAE"
     gene            complement(2487..3392)
                     /locus_tag="VFY27_07120"
     CDS             complement(2487..3392)
                     /locus_tag="VFY27_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5765321.1"
                     /translation="MRAASRLALLLAAWPCSGPAQELEPRFYSNAPVGVNVLVAGYAY
                     SEGSVLVDPSLALDNADLAIDGPYVGYARSMSIGGRTGKFDVGIANACIDGSAEFEGE
                     PVSRKVCGWSDAKLRFSLNFLGSPALSPREFAAYEQDLVVGATLLLTAPIGRYDPERL
                     VNIGTNRWAARTELGMSKGLERWLLEASVSASFYETNSDFFGGRRRKQDPIYAFQAHV
                     VRRLNRGVWVALDYIRYRGGQTETDGLDDANLQSNARVGFTLSVPVARRHTVRLSASS
                     GLLTRTGTDFDTIGLSWAYIWGRGL"
     gene            complement(3429..4082)
                     /locus_tag="VFY27_07125"
     CDS             complement(3429..4082)
                     /locus_tag="VFY27_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5765322.1"
                     /translation="MRAASIALSLLVFPALALAQGYGSGAGAWELSAVGLYQDAETAT
                     TGGDSMVSVDEAFGLGFGFGYYFTDRLVLGADFEWLSPDYSATLVNDSGNTSSFDHEL
                     TQINVRFKGAFYFLEGPFSPYAELGAGWTYVDSNVADGPPVTGCWWHPWFGYICDNYY
                     DTFDETSFSYGGALGLRYRFQGGTVLRLSYNHYEVDGSGDAPDPTLNAARLEFVWGF"
     gene            4352..4945
                     /locus_tag="VFY27_07130"
     CDS             4352..4945
                     /locus_tag="VFY27_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX5765323.1"
                     /translation="MNLKKKGVFAAAILSLATIPGCASGPDIRSDFDRTADFSTYETF
                     GFASELGTDRAGYTSLITEHFKRAIRRELETRGYEYTETNPDLLANFYVSVREQTDIR
                     STPSTTAGAGYYGYRYGMYTGWPAYTTDVRTVHYKAGTANIDLVDAKRKQLVWEGVAE
                     GRVRDEALENPGPAIDAVVAELFARYPARAGSAASAD"
     gene            5023..>5513
                     /locus_tag="VFY27_07135"
     CDS             5023..>5513
                     /locus_tag="VFY27_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX5765324.1"
                     /translation="MRSRLRPGSLCLLFLGGCAQLPARPELPTESGIAVAAEDTVLDR
                     RAGELESRNPAASAFRLVSEGPEAFAIRAHSADLAGRSIDVQTYIWHADVTGLYLAHR
                     LLEAADRGVSVRLLLDDMDARAKSAGLAALDAHPDIRIRVFNPFATRRGILRQLGELV
                     GSFG"
BASE COUNT         1108 a         1738 c         1690 g          977 t
ORIGIN      
        1 aatttccgcg cgagccgcgc ggccaggaac ccgccatcga tgtgccagtc ggccctccct
       61 tcccccgccg ccccgtcccc ggtcaaaagc gtcagccgga gatcggctgc gccgattgcg
      121 ccggtcaagc gccagcggtc ctcagcccag taactctgct gggcgagcac cagcacccgg
      181 ctgtcgttgc tggtatacat tgcggccgca ccggtcacgg atgcgggctg cctggctgcc
      241 tgttccgccg tctgcggcca gaaatatgcc gcacccgcga tcagtccggt gtcgagcgtc
      301 ggattcgaga tcggtatcgg caccacgacg taattgccac gttggagttt caggttggta
      361 ctgtcctcgc gaatatccgg ggcaataccc tggtgggttt ccgtggagga aggttccgcg
      421 ttggcgcact cgagagccag cagggtgagt gccagtagcc ggatcaaatg gccatacgct
      481 aaatgtcggc gtccgtcacg tcggtgcacg agccgaatac cgataccgac aggccgtcac
      541 gggacaccgc gaccgtcagg tgcccggtgt gttcgtgcac gacgaagctg aatgcgcgac
      601 ctccttcgat accctggaag aacagcaggc cgtcggcacg gttaatttca ccgatagcag
      661 tggatcggtt ctcaccgctg gctttcgtcg ttgtgagccg tttcttcctg gtatcgacca
      721 cgacgaattg tggcacgtcg agttcctcgg ccagaagctc cgcacaatca ccggtttcaa
      781 agcaggccat gatccgcgag gtggaacaga gcaatcgttc gctgccgcta atgtcgtcgg
      841 cccgggaggt ggagacagca agaatgacga gtgcgacggc caggatccga ttcatatcga
      901 acttcctctc gcttgccggg ctgcttcacc cgtgggcaca gggaccagcg accagtctag
      961 tcaatcgccg cacgaccgga tatcaatatc gcgcagtgcg gaaacccgat acgacacaca
     1021 ttccgggtca ccgtggttct cactcggcga gcggcaattg cacgcggaga gtcgtgccgt
     1081 aacccccgtt ttccgcccag attcgtcccg cgtgtgcttc cacaattatg cgtgaaatgg
     1141 acaggcccat tcccatacct gccgccttcg tcgtgtagaa gggttcaaag atacggccta
     1201 atgagtcggg aggaattccg cggccggagt cgctgattgc aagctcgacc cagctgccat
     1261 cgatggtgca cgcgcggacc gacacatgcc ggcgcccatc cggcaggtcc gcgaccgcgt
     1321 ccattgcgtt gatgatgaga ttgatgagca cctgcgaaag gtggaccacg tcgccgtgga
     1381 ccatgcccgt gcctgcatcg ctggcacatt ccagcctcac gttgcgcgcg atggcgtcgg
     1441 atttcagcag cgcggatgta tcctccaaca agcgctctac tgacacgggc gtgaaatcaa
     1501 gactgcgcct cttgagcaac gagcgcaaat gatcaatcac ctcgatggcg cgctggttgt
     1561 cgcgtcgaat gtcgtcgagt atctgccgca gctcctcgag atccgggttc ggccccttca
     1621 gcagctgagc gcccagcctg gtattgatca ggatggcgct catcggctga ctcaattcgt
     1681 gcgtgagaga cgacgacaag gttcccagcg cagacactct accggcgtgc gccaactcgc
     1741 gctgcagctc ctcgactttc tgacgagtcc tgagcgcgtc gcggcccaat tcgaaggtca
     1801 tcgccgccag cacgatcata ctcggcggcg aaagcagcgc gggcacgtga acgttgccat
     1861 agatcattag ctgagtgagc acgatgccaa tggccaagga gaggaagacc gccccgccga
     1921 tcactatgac cttacggcgt gcctcgtacg ttccacggcg ccacagcgta aacgacgcgt
     1981 caccgacgaa cgtcaggacc agaatggtag aagcggtcgc gacccactgg tagggactga
     2041 ccaccgcctg tccgacgatg gtcacctgtt cgccgaggaa agcaacgcga tcgatgctgt
     2101 cgatgctcgc aaagttgaag ttcggatcgc tcacgaagcc ggcgatcagg atgatcgccc
     2161 ggcttgtgat gatggtccac atcagccagg acctccccgt cttgaaatag aagcggatga
     2221 aggcgagcag ggcgaccgta cgcagaaata tcggtatctg gaaccaccgc agccactcgc
     2281 cccactccgc agccgtcgtc gagtacatca tgccgagttc gaatatgacg atgccgacga
     2341 tggccagcgc ttcaaaggca aaggccaggt aggcgtacgc cttgcgatcc agcgcccaaa
     2401 cggctccgta catgatcgcg agcagcaggg caccggtcgc cacggccgac cagagaatcg
     2461 taacgtgact catcgggaga taatgcctag agaccccggc cccagatgta tgcccaactc
     2521 agcccgatgg tatcgaaatc ggtccccgtc cgggtgagca ggccgctgct ggcgctcaat
     2581 ctcaccgtgt gccggcgagc caccggcacc gacagggtaa acccgacgcg cgcgttggat
     2641 tgcaggttgg catcgtccag cccgtccgtt tcggtttgtc cgccccggta ccgtatgtaa
     2701 tcgagcgcga cccagactcc ccggttcagc ctgcgcacga cgtgagcctg gaacgcatag
     2761 atcggatctt gtttgcgtcg tctgccgccg aagaagtcgg agttggtttc gtaaaagctt
     2821 gcgctgacgc tggcctcgag cagccagcgt tccagtccct tcgacatacc gagttccgta
     2881 cgcgcggccc aacggttcgt gccgatgttc accagccgtt ccggatcata tcgaccaatc
     2941 ggcgcggtga gcagcagtgt ggcgccgacc acgagatcct gctcgtacgc ggcgaattcc
     3001 cggggcgaga gagcgggcga gcccaggaag ttgagcgaga agcgcaactt cgcgtcgctc
     3061 cagccgcaga ccttgcgact gaccggctcg ccctcgaact cggcgctgcc gtcgatgcat
     3121 gcgttcgcga taccgacatc gaatttgccg gtgcggccgc cgatcgacat ggagcgcgcg
     3181 taaccgacat agggaccgtc gatcgcgaga tccgcgttgt cgagcgcgag agaggggtcc
     3241 accagcacgc taccttcaga atatgcgtag ccggccacca gcacgttgac gcccaccggt
     3301 gcgttggagt agaagcgcgg ctccagttcc tgggcgggac ccgaacatgg ccaggcggcg
     3361 agcagcagcg caagacggct cgctgcgcgc atcgaagcgg tgccggctgt gggagccgcc
     3421 tgcccggatc agaaccccca gacgaactcg agtcgggccg cgttcagcgt gggatccggc
     3481 gcatcgccgc tgccatccac ctcgtagtgg ttataactga gcctgagcac cgtgccgccc
     3541 tggaaacggt agcgcagccc cagcgcacct ccgtaactga agctcgtctc atcgaaggtg
     3601 tcgtagtagt tgtcgcagat gtagccgaac cagggatgcc accagcagcc cgtgaccggc
     3661 ggcccgtccg cgacgttcga atccacgtag gtccagccgg cgccgagctc cgcgtagggc
     3721 gagaaagggc cctcgagaaa atagaaggca cccttgaagc ggacgttgat ctgcgtcagc
     3781 tcgtggtcga acgacgaggt gttgccgctg tcgtttacca gggtcgccga gtaatcggga
     3841 gacaaccatt cgaagtcggc gcccaggacc aggcggtcag taaagtagta gccgaagccg
     3901 aaacctaggc caaaagcttc atcgaccgac accatcgaat cgccaccggt agtggctgtt
     3961 tcggcatcct gatagaggcc gacagcagat agctcccagg cgcctgctcc gcttccataa
     4021 ccctgcgcca aggccagtgc ggggaaaacc aacagcgaca gggcgattga tgcggcacgc
     4081 ataattttcc tccgtggacg ttcgacgatg catcgggcac gattcctgac ccgcatggcg
     4141 gcccaaaaaa aacccgaacg ccgttcgaaa tcactgatgg aagtgtgacg agcggcagtt
     4201 tgcgccgata tcaacaaaac gcacgggttg gggcaggtgg attgatgaga acggcaattg
     4261 ccgggatact aggcttgagt tgtgtgagca ccgagatatg gatgcgaaca ggtttgattc
     4321 cgcgggacgg tagtttatct ggagaagaaa cgtgaacctc aaaaagaaag gtgtattcgc
     4381 agcagcgatt ctgagccttg caacgattcc gggatgcgcg tccggccctg acatccgctc
     4441 cgacttcgat cgaaccgctg atttctccac ctatgagacg ttcggattcg cgagcgaact
     4501 cggtaccgat cgcgctggct acacgtcgct catcacagaa catttcaaac gcgcaatcag
     4561 gcgcgagctg gagacgcggg gatacgagta caccgagacg aacccggacc tgctcgcgaa
     4621 tttctacgta tccgttcgcg agcagaccga tatccgctcg acaccgtcga caacagcagg
     4681 ggcgggctat tacggttacc gttacggcat gtacaccggc tggcctgctt acacgaccga
     4741 cgtacgcacc gtgcactaca aggcgggtac ggccaacatc gacctggttg acgccaagcg
     4801 gaagcagctc gtctgggaag gcgtggccga gggccgcgta cgggacgaag cgctcgagaa
     4861 tccgggtcct gccatcgatg cagtcgtcgc ggaactgttc gcacgctacc cggccagggc
     4921 cggttcagcg gcgtccgcgg actagcgaca ctgcacggac ggcatcgtct tcggaagagc
     4981 agtcgacggc tcgccggggc ggtggaccag ccgtagctcg tgatgcgatc ccgtctgcgg
     5041 cccggatcgt tgtgccttct tttcctcggc ggctgcgcac agcttcccgc acgccccgaa
     5101 ttgcctaccg agtccggtat cgcggtcgcg gccgaagata ccgtgctcga ccggcgcgcc
     5161 ggggagctcg agtcgaggaa tccagcagcc tcggcctttc gactggtgag cgaggggccc
     5221 gaggcgttcg ccatccgcgc gcacagtgcc gacctggcag ggcgctcgat cgacgtgcaa
     5281 acctacatct ggcacgccga cgtcaccggc ctgtacctcg cccaccggct gcttgaagct
     5341 gccgatcgcg gggtgtcggt gcggctgttg ctcgacgaca tggacgcccg cgcaaaaagc
     5401 gccggccttg ctgcgctcga cgcacatccc gacatccgta tccgcgtgtt caatcccttc
     5461 gcgacgcgcc gcggcatcct gcgccagctg ggggagcttg tcggcagctt cgg
//
LOCUS       DASUOM010000337         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_990768,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000337 DASUOM010000000
VERSION     DASUOM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990768"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFY27_07140"
     CDS             complement(<1..361)
                     /locus_tag="VFY27_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5765325.1"
                     /translation="MLTLENVACSYGAEPVLRDVSFHLERGNVGCILGPSGCGKTTLL
                     RCIAGFERITNGEIIAAGTTLSTPSRHLPAEQRRIGMVFQDYALLPHLTALKNVEFGL
                     RGMSAAQRRARAMQFLQQ"
     gene            complement(361..1992)
                     /locus_tag="VFY27_07145"
     CDS             complement(361..1992)
                     /locus_tag="VFY27_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5765326.1"
                     /translation="MRVAATAPAGRLNSVGGVWPRVVVWLVAFMAALPVLVALLSFFS
                     ASAGVWPHLARYVLPEAFFNTIWLVTGVAVCTSVLGVSLAWFVASCEFPGRRIFNWAL
                     LLPMAMPGYVLGFAFAALFEFTGPVQGAWRGLCGASTPFPDIGPAGASILTLSLVLYP
                     YVYLIVREAFASQGIRGMEVARGFGYRPLQAFFRVSLPMARPWLVAGVSLAVMECLAD
                     FGTVKLFNYTTFTTAIYRAWYGLFSLQSALQLSLVLVTLVLIALVLERRFRGKSRYTP
                     PGAGRERRIRLSRGRAAAVTVYCTAVLGVAFGLPVSLIVFWSVENFAAEFDARYWQLA
                     LNTLVLASGAAAMITSIAIMLGVTVRRAPTMLNIGLARVATLGYAIPGTVLAVGFFVP
                     IAWLSRIVNQYFGSETGTVVALQSGLSVIFLAYLARYLAVAHGPVDSALLRIRPGIEE
                     AARGMGVSGLRLLARVHLPILRPAILTALLLVFVDIMKELPITLMTRPFGWDTLAVKV
                     FQLTTEGEWHRAALPALAIVVVGLMPVMLLSRQRR"
     gene            complement(2018..3082)
                     /locus_tag="VFY27_07150"
     CDS             complement(2018..3082)
                     /locus_tag="VFY27_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5765327.1"
                     /translation="MRKALRSRPQGLLLVILSSILGACGGGDQQSAPVTEATGAGGSP
                     EPVVVYSARAEQLIKPIFDAYTAETGVPIRYTTDSEQPLIQKLLAEGETTPADLLMTV
                     DAGNLWYAAEQGTLRPTYSDVLEANIPEHLRDPGNRWFALSVRARTIVYDTRDVDPSE
                     LSDYRGLAEEKWQGKLCLRTSEKVYNQSLVAMLIADHGEEQTERTVRGWVENLATDVF
                     SNDTSLIEAIAAGQCDVGIVNTYYFGRLQKENPDIPVAIFWPPAETGGVHVNVSGAGV
                     TRHAKNPEGAVALLEWMSSATAQKLLGGENMEYPANPDIEPHPVVAAWGTFKANPMNV
                     ARAGEYQADAVRLMDRAGYR"
BASE COUNT          682 a         1065 c         1038 g          547 t
ORIGIN      
        1 cctgttgcag gaactgcatc gcgcgcgctc ttctttgggc cgcggacatt ccacgaagac
       61 cgaactcgac attcttcaaa gcggtcaggt gcggcagcag ggcatagtcc tggaacacca
      121 tgccgatccg gcgctgttca gccgggaggt ggcgcgaagg ggtgctcaac gtcgtgccgg
      181 cagcgattat ctcgccgttc gtgatccgtt cgaagccggc gatgcatcgc agcagcgtcg
      241 tcttgccgca gccgctcggg ccgagaatgc aacccacgtt cccccgctcg aggtggaacg
      301 acacgtcccg cagcaccggt tctgcaccgt aactgcaggc aacgttttca agcgtcagca
      361 tcagcggcgt tgccggctca gcagcattac cggcatgagc cccacgacca cgatcgcgag
      421 cgcgggcagc gcggcacgat gccactcacc ttcggtcgtc agttggaaga ccttcacagc
      481 cagcgtgtcc cagccgaagg gacgggtcat gagcgtgatc ggcagctctt tcatgatgtc
      541 gacgaatacc agcaacaagg cggtcaggat tgcgggacgc aggataggca ggtgcacgcg
      601 ggcaagcaac ctgagacccg atacccccat gccgcgcgcg gcttcctcta tgccggggcg
      661 aatcctcaac agcgcgctgt cgaccggtcc gtgcgccacc gccaggtagc gcgcgaggta
      721 ggcaaggaaa atgaccgaaa gcccgctttg caaagcgact accgttcccg tttcgctgcc
      781 gaagtattgg ttgacgatcc ggctgagcca tgcaatggga acgaagaagc ccacagcgag
      841 cacggtgccc ggaatcgcgt aacccagggt agcgaccctg gccagaccga tattcaacat
      901 ggtaggcgcc cgccgcacgg tcacgccgag cattatggcg atagaagtaa tcatcgcggc
      961 ggcgcccgag gcaagcacga gcgtattcaa ggccagttgc caatagcggg catcgaactc
     1021 cgcggcaaag ttctcgacac tccagaatac gatcagcgag accggcaggc cgaatgccac
     1081 ccccagtact gccgtgcagt acacagttac tgctgctgcc ctgccgcggg acaggcggat
     1141 gcgccgctcc cggccggcgc cgggcggcgt gtagcgggac ttgccgcgaa aacgccgctc
     1201 gagcaccagc gcaatcagta ccagggttac caggacaagc gacagctgga gggcggactg
     1261 cagcgaaaac agaccgtacc aggcacggta gatggcggta gtaaaagtcg tgtagttgaa
     1321 gagcttgacg gtgccgaagt cggcgaggca ctccattaca gccaggctta cgcccgccac
     1381 cagccacggg cgcgccatcg gcagcgacac gcggaagaaa gcctgcagcg gcctgtagcc
     1441 aaacccgcgg gcgacttcca tgccgcggat gccttgtgac gcgaaggcct cacgaacgat
     1501 caggtaaacg tacgggtaca gcaccaggct caaggtaagg atagacgcac cggccggccc
     1561 gatgtcgggg aacggtgtgg aggcgccgca caggccgcgc caggcgccct gcaccggccc
     1621 ggtgaactcg aacagcgcag cgaacgcgaa acccagcacg tatcccggca tcgccatcgg
     1681 caagagcaat gcccagttga agatgcggcg tcccgggaat tcgcaggacg cgacgaacca
     1741 cgccagcgaa actccaagga cggaggtgca aactgccacc cccgtcacca gccatatggt
     1801 gttgaagaac gcctccggga gtacgtagcg cgcgaggtgc ggccagactc ccgcggaagc
     1861 cgaaaagaaa gacaacaagg ccaccagcac cggcagtgcc gccatgaacg cgacaagcca
     1921 gaccaccacg cgaggccata cacccccaac gctgttcaaa cgtccggcag gagccgtggc
     1981 cgcgacgcgc atggcgcctc aggattcttg tcgaaaatca gcgataacca gcccggtcca
     2041 tcaagcgcac agcatccgcc tggtactcgc cggcccgggc gacattcatc gggttggcct
     2101 tgaatgttcc ccaggctgca acgactggat ggggttcgat atccggattt gccgggtact
     2161 ccatgttctc tccgcccaag agcttctgcg ctgtcgcgga agacatccac tcgagcaggg
     2221 cgacagcccc ttccgggttc tttgcatggc gcgtcacgcc cgctccggac acattgacgt
     2281 gcacgccacc ggtttccgca ggtggccaga agatcgcaac cgggatgtcg ggattttctt
     2341 tctgcaggcg cccgaagtaa taggtgttca cgataccgac atcacactgc cccgcggcga
     2401 ttgcttcgat gagactggtg tcgttcgaaa aaacgtcggt tgcgaggttt tcgacccacc
     2461 cgcgaacagt cctttcggtc tgctcctcgc cgtgatcggc gatgagcatc gcgaccagcg
     2521 actggttgta gaccttctcc gaggtgcgca ggcaaagctt tccctgccac ttctcttcgg
     2581 caagcccgcg gtagtccgaa agctcggacg ggtcgacgtc ccgcgtgtca tacacgatcg
     2641 tgcgggcacg caccgacagg gcaaaccacc ggttcccggg gtccctcagg tgctccggaa
     2701 tattggcttc gagaacatcc gaataagtag ggcgcagcgt gccctgctcg gcggcatacc
     2761 agaggttgcc ggcatcgacg gtcatgagca ggtccgccgg cgtcgtctcg ccctcggcca
     2821 gcagtttctg gataagcggc tgttcgctgt ccgtggtgta gcggatgggt accccggtct
     2881 ccgcagtgta ggcgtcgaag atcggcttga tcagctgctc cgcccgcgcc gagtatacga
     2941 ccaccggttc tggcgagccg cctgccccgg ttgcctcggt tacaggcgca ctttgctggt
     3001 cgccgccgcc gcaggcgcca aggatcgacg acaggatgac aagaagcagc ccttgcggcc
     3061 gcgagcgtag cgcctttcgc atgatcagtg cccctccccg cttacgggga attcgagtta
     3121 atgataatgg ttctcattat cacatatcga cggcaggcat ggccacagac gcgggggaat
     3181 caggtctcgc tcaccatgat ccggttgcgg ccagccgatt tggcgctgta gagggccacg
     3241 tcggcccggg cgatgaggct ttcgccgagg gtcttcaggt cgaagtcgct ttcctgcgga
     3301 gtggcgctcg cgatgccgat cgaggcggtg at
//
LOCUS       DASUOM010000338         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_74706,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000338 DASUOM010000000
VERSION     DASUOM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74706"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFY27_07155"
     CDS             complement(<1..271)
                     /locus_tag="VFY27_07155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5765328.1"
                     /translation="MRVDAARRLQQIWSTIRDIPEGKVASYGQIAELAGIPRGARQVG
                     RALGQVPDGYKVPWHRVLRSSGQLAFDRNSATFREQASLLAEENVP"
     gene            complement(261..1637)
                     /locus_tag="VFY27_07160"
     CDS             complement(261..1637)
                     /locus_tag="VFY27_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5765329.1"
                     /translation="MSILKRVFGKLPKWRSRSKERSVEAANGSDGGSRHLTLARESLR
                     ELIADSRLPPGIREALARDYTEVQAMLDKLEHGHLHIAAFGRVSTGKSSLLNALIGEE
                     RFTVSPLHGETRTSSMQPWSEVEAGGVFLIDTPGLDEAGGEEREMLATEVANRADLVI
                     FVLDGDITESERASLRTLLKQGRPVLVALNKKDLYTQADLEALLASIRAKTEGLVSAG
                     NVLAVAADPRPQAIIEIDESGVETESVRQRKPDIEDLKARLWDIFEAEGKTLAALNAS
                     LFAADLSDQVGRRILEARREIGDRLVRTYCVAKGIAVAFNPVPVADLFAAAFIDVGMV
                     VHLSKVYGLPLSRREAGSLVRVIAAEAAALMGTVWALHFVSSALKVGTLGLSTILTAG
                     AQGAIAYYSTYLVGRVAAEYLAHGKSWGDGGPKRVVKQILDSLDKETVLQDARREIRA
                     RLGAADAR"
     gene            complement(1549..3066)
                     /locus_tag="VFY27_07165"
     CDS             complement(1549..3066)
                     /locus_tag="VFY27_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX5765330.1"
                     /translation="MKVDRFIRLAAGFLILLVFVIAIAALLFVTESALNVWDRLAAGP
                     KVILYGYLSAMAALIAAAAWLVWRLVVRRSPKESARKARPSRLTREEIESRLRAADEA
                     GVDTSAALAELHELAARQREQAVHLCFFGEISTGKSSLIKALVPDADVKVDAVGGSTL
                     AITHYQWENESGVRVLLTDVPGTGGIVEGLDKIAETEARRAHVVVFVCDGDLTRSEKQ
                     AVKWLLELEKPLVLVMNKSDRYDAREQSMLMQRLLDHLSDLGGDVARDRVVAVSAGGR
                     TEVIAKRPDGSEQIVPRERPADVGVLIVAINRLLEESADEIDARRDRAVFGLAAEKLA
                     DAEQRFRDQRSEQIVRNSTRKAIIGALAAISPGTDIIIQSYVGTTMTRALCKLYGAAP
                     RDLDIEEFLALSQSRVGRALPLTLAVAGNGLKAFPGIGTVAGGLVHAVAYGLIFDALG
                     RSLVHTLRKHGELKPETAAKEFEEGISEHIEAGVRQVAKMALSVERKERRSGERE"
     gene            3627..>4976
                     /locus_tag="VFY27_07170"
     CDS             3627..>4976
                     /locus_tag="VFY27_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765331.1"
                     /translation="MMTRGETSNRYRWQGFLPLLLVLFAAAVAHAQREDVYVPGELRP
                     WVDWVLQDKEYLECPFFFDQGNAAADNFICTWPGELELDVTGSGAGFSQGWTIYASEQ
                     WVPLPGDANAWPEQVTANGQAVEVVLKSNTPSVRLAPGQYTLTGRFAWSEAPRVLNVP
                     ARTGLLSLTVDGSPVARPERNQDSVWLAERETQIAAQDSLDVQVYRLVADDVPTRLTS
                     ILNLDVSGNVREVVLGPVLPGDFMPLSLDSPLPARLEPDGQLRLQVRPGSWQVRLVAR
                     GSGVLNEVTLPAGTRAMPDAEIWSYRSNDRLRVTIPEGTVPVDPGQVSTPDEWLDLPA
                     FRIQPGETLAITEQSRGRLAAGNQLQLNRQLWIDFDGSGFVFADTITGTMRSDWRLDV
                     VEPYVLLSASEDGQNLLVTLGPEDGMTGVELRQRDVGLQAIGRSETRGTLPATGWQTR
                     "
BASE COUNT          887 a         1668 c         1506 g          915 t
ORIGIN      
        1 ccggcacgtt ctcttcggcc agcaggctgg cctgttccct gaaagtcgcg ctgttcctgt
       61 cgaacgcgag ttgaccggac gaacgcagaa cgcgatgcca ggggaccttg taaccgtccg
      121 gaacctgtcc gagggcacga ccgacctggc gcgcgccgcg cggtatgccg gccagctcgg
      181 cgatctggcc gtaactcgcg accttgcctt cggggatgtc gcgaatcgtg ctccagatct
      241 gctgcaaacg gcgcgcggca tcaacgcgca tctgctgcac ccaggcgcgc acgaatctcg
      301 cggcgtgcat cctgcagcac ggtctccttg tcgaggctgt cgagaatctg cttcaccact
      361 cttttcggac cgccgtctcc ccaggacttt ccgtgcgcaa gatattcggc agcgacacgg
      421 ccgaccaggt acgtactgta gtaggcaatc gcgccctggg cgcctgccgt aagtatcgtg
      481 gacaggccga gcgttccgac tttaagcgca ctcgacacga agtgtaatgc ccacacggta
      541 cccatcaaag ccgccgcttc cgccgcaatt acacgcacca gcgagccggc ttccctgcga
      601 ctcaaaggca acccgtaaac tttcgaaaga tgcacaacca tgccgacgtc gatgaaggcc
      661 gcggcgaaaa ggtccgccac cggtaccggg ttgaaggcga ccgcgatacc tttcgcaacg
      721 cagtacgtcc gcaccagcct gtcgccgatt tcgcgccgtg cctccagtat ccgtcgtccc
      781 acctggtcgc ttaaatcggc cgcaaacagg ctcgcgttca gcgcggcaag cgtttttcct
      841 tctgcttcga agatatccca cagacgtgct ttgagatcct cgatgtccgg cttgcgctgc
      901 cgtacgctct ccgtctcgac gccggactcg tctatctcga tgattgcctg cgggcgcgga
      961 tcggccgcaa ccgcaaggac gtttccggca ctcaccagtc cctcggtctt cgcgcgaatc
     1021 gaggccagca gcgcttcaag atccgcctgt gtgtagaggt cctttttgtt caacgcgacg
     1081 agcaccggcc ggccctgttt caggagcgtg cgcagcgacg ctcgctcgct ttcggtaatg
     1141 tcaccatcca gcacgaagat cacgaggtcc gcccgattcg ccacctccgt cgcgagcatc
     1201 tcccgctcct cgccgcccgc ctcatcgagg cccggcgtat cgatcagaaa tacgccgcct
     1261 gcctccacct ccgaccacgg ctgcatgctc gacgtccgtg tttcgccgtg cagcggactg
     1321 accgtgaatc gctcctcgcc gatcagcgca ttcaaaagag aggacttgcc ggtgctgaca
     1381 cggccgaacg cggcgatatg caggtgcccg tgctccagct tgtcgagcat cgcctgcact
     1441 tccgtgtaat cgcgcgcgag cgcttcgcgg ataccgggcg gcaggcgcga atcggcgatc
     1501 aactcgcgca ggctctccct ggcgagcgtc aggtggcggg aaccgccgtc actcccgttc
     1561 gccgcttcga cgctcctttc tttcgaccga gagcgccatt ttggcaactt gccgaacacc
     1621 cgcttcaata tgctcactaa tgccttcctc gaattctttc gcggctgttt ccggcttcag
     1681 ttcaccatgc ttcctgagcg tatgcacgag actgcgcccg agcgcatcga atatcagccc
     1741 gtacgcgacc gcgtgcacga gcccgcccgc gaccgtgcca atcccgggga aggccttgag
     1801 gccgtttccg gccaccgcca gcgtcagcgg caatgcacga ccgacgcgac tctggctcaa
     1861 tgccaggaac tcctcgatgt cgaggtcgcg cggcgcagca ccatacagct tgcacaacgc
     1921 ccgcgtcatc gtggtgccga cgtagctctg gatgatgatg tcggtgccgg ggctgattgc
     1981 ggcgagcgcg ccgatgatcg ccttacgcgt cgagtttcgt acgatctgtt ccgaacgctg
     2041 gtcccggaag cgttgctctg cgtcagcgag tttctcggct gccaggccga agacagcgcg
     2101 gtcccgacgt gcgtcgatct cgtcggcgct ttcctcgaga agccggttga tcgcgacgat
     2161 cagaaccccg acatccgccg gtcgttccct cggcacgatc tgttcactgc cgtcgggcct
     2221 ttttgcgatt acttccgtcc ttcctcccgc ggacaccgcg acgacgcgat ccctcgcgac
     2281 atcaccgccg agatcgctca ggtggtcgag aaggcgttgc atcagcatcg actgctcgcg
     2341 cgcatcgtaa cgatccgact tgttcatgac gagcaccagc ggcttttcga gttcgagcag
     2401 ccacttcacc gcctgcttct cggagcgggt caggtcgccg tcacaaacaa agacaacgac
     2461 gtgggcgcgc cgtgcttcag tctcggcaat cttgtcgagg ccctccacga tgccccccgt
     2521 tcccggcaca tccgtcagca ggacgcggac gcccgattcg ttctcccact ggtaatgcgt
     2581 gatcgcgagg gtcgagccgc cgaccgcatc cactttcacg tcggcgtccg gaacgagagc
     2641 ctttatgagt gaactcttcc ccgtgctgat ctcgccaaaa aagcacaggt gcactgcttg
     2701 ctcgcgctgc ctcgccgcaa gctcatgcag ttccgccaaa gccgcgctgg tatcgacgcc
     2761 cgcctcgtcg gcggcgcgca ggcggctctc gatttcctcg cgggtgagtc tggaaggacg
     2821 cgccttcctt gcgctctcct tcggactgcg acgaaccacc agtcgccaca cgagccaggc
     2881 ggccgcagca atcagcgctg ccatcgcaga gaggtagccg tacaggatga ccttcggtcc
     2941 cgccgccagc ctgtcccata cgttgagcgc agactccgtg acgaacagga gcgcggcgat
     3001 cgcaatgacg aacaccagca gtatcaggaa gcctgccgcc aggcggatga accggtcgac
     3061 tttcatgggg catgagactg cccgaatgtg gtgcttttgt cttgtttcag ccgctcgggt
     3121 ttggcgcgtt ccgtcctcac tgctaggggc gggggatcac cggccgcgtc gcgccgtgtt
     3181 gcgaggtcga cacgcagctg caggagtcat aatccttgcc gaaggtccca tggtggatac
     3241 gaccggtcgt ccgcacgtcg caggttattg tgtagccggc cgggcaccgc agtcctgcct
     3301 cgcttgccgt ggggtcgatg cggccgctcg tcccacctcc gccggcgcac ccgcagagga
     3361 cgagcgcaag cacagttgct gcgaacagag aacataccgg gcgcttcgtc ggctgcatgt
     3421 ctgtcacggg gtgcgccgag tgcaggcaag gcctcgggag tgcaccgtca gggagcaaaa
     3481 tggagcggta gcctgagccc cgagtatagc aatcggaaac gccggggcgg cagcggccgg
     3541 gcgtcgggtg tggcaatatt cccgcgccgc gcattccggt tatagtgtct ttacaaacaa
     3601 cagcgaagaa caagaacggg gacaaaatga tgactcgagg cgaaacctcc aacaggtatc
     3661 gctggcaagg atttttgccg ctgctcctgg tgctttttgc cgctgcggtt gcgcacgctc
     3721 agcgggagga tgtgtacgtt cccggggagc ttcgcccgtg ggtcgactgg gttttgcagg
     3781 ataaggagta tctcgagtgc ccctttttct tcgaccaggg caatgccgcc gccgacaact
     3841 tcatctgcac ctggcccggt gaactcgagc tcgacgtcac cggctccggt gccggcttct
     3901 cacagggatg gaccatctac gcgagcgaac agtgggtgcc actgccgggc gatgctaacg
     3961 cctggccgga gcaggtgacg gccaacggcc aggcggttga agtcgtcctc aaaagcaata
     4021 cacccagcgt tcgcctggca cccggccagt acactctcac cggccgtttc gcctggagcg
     4081 aagcgccgcg cgtcctcaac gtgccggcaa ggaccggcct gctgtcactc acggtcgacg
     4141 gcagcccggt agccaggccc gaacgcaacc aggattctgt gtggctggcc gagcgcgaaa
     4201 cacaaattgc agcgcaagac agtctcgacg tgcaggtgta tcggctggtg gcggacgacg
     4261 tgcccacgcg cctcaccagc atcctgaacc tggatgtatc aggcaatgtg cgcgaggtcg
     4321 tcctcggccc ggtcctgcca ggagatttca tgccgctttc gctggacagc ccgctgcccg
     4381 ctcgcctgga accggacggg cagttgaggc tgcaggtccg gcccggaagc tggcaggtgc
     4441 gcctcgttgc ccggggcagc ggggtgctga acgaggtcac gctgccggca gggacaagag
     4501 ccatgcccga tgccgaaatc tggagttatc gcagcaacga tcgcctccgc gtcaccatcc
     4561 cggagggcac ggtaccggtg gatccgggac aggtatccac gccggacgag tggctcgacc
     4621 ttcctgcgtt ccgcatacag ccgggcgaga cgctcgccat caccgagcag agccgcggca
     4681 ggcttgcagc cggcaaccag ctgcaactga accgccagct ctggattgat ttcgacggca
     4741 gcggtttcgt ctttgcggac accataacgg gcacgatgcg aagcgactgg cgcctcgacg
     4801 ttgtggagcc ctacgtgctg ttgagcgcct ccgaagacgg gcaaaacctg ctcgtcacac
     4861 tgggacccga agatggcatg acgggagtgg agcttcggca acgcgacgtc ggcctgcagg
     4921 caatcggccg cagtgaaacc cgcggcacgc ttcccgcaac cggctggcag acgcgc
//
LOCUS       DASUOM010000339         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_373740,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000339 DASUOM010000000
VERSION     DASUOM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373740"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VFY27_07175"
     CDS             <1..1624
                     /locus_tag="VFY27_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5765332.1"
                     /translation="MFGGTGPPVRRADMAIATFLCILFAACGGDADRGSGYALDRGLG
                     AEPESLDYHKARSSQAGDVQRDLGEGLTGYTPEGELVPRAALRWEVSDDGLEYRFWLR
                     PDARWSTGEPVTAEDFVYSFRRLVAPATASFYSQSIIDVRNAEAILAGEKAVEELAAT
                     AEGEFLLHLGLARPAPYFLALLTHPSMFPVHRASVERHGDGFARPGNLVTNGAYRLVE
                     WELGSLIELQRNENYWDNASTAIDRVRHHFVAEPAVELNRYRAGELHITSTVPPEAFA
                     RMRETRPEELRVAPYLNVYYYGFNLTKPPFKDNPELRRALSMAIDREALTESVTGRGE
                     TPAWSWVPPGVDNYSERRFPYSTMSDEEREAAAKRAYRSAGYGDGEPIEIEIRYNTSD
                     THQRIALAIQSMWRDVLGVEATLVNEEFQVLLSNMRAKEVTQVFRSSWLGDYNDANTF
                     LQVMLSDAPSNLTGYANPDFDALVAQAAAQTDPEERRRYMQEAESLLLADHPVIPIYF
                     FVSKHLVSPRIGGWGDNVLDYHYSQHLSLLPE"
     gene            1643..2566
                     /locus_tag="VFY27_07180"
     CDS             1643..2566
                     /locus_tag="VFY27_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5765333.1"
                     /translation="MLRFALLRILSAIPTLFLVVALAFLMVHAAPGGPFDQERVLPPD
                     NARNLAAAYHLDEPLPRQFVRYVSGLVRGDLGPSYYYRDHDVSDLIGAALPLSLQLGL
                     LSMAVALVAGIFAGIAAALKQDTLLDRFVTGLAMTGISVPVFVVAPLLVLVFSVQLGW
                     LPASWSGSAGWSRLLLPVLALSLPQIAYVTRLTRASMIEVLSSDFIRTARAQGLSTVS
                     IIRYHALKPAMLPVVSYLGPATAAVLTGAVVVEIVFGVPGLGQLFVRSALNRDYTLVL
                     GIVIFYAALIILLNLLVDLLYGILDPRIRES"
BASE COUNT          476 a          839 c          866 g          545 t
ORIGIN      
        1 catgttcggg ggcactgggc caccggtacg gcgcgcggac atggcgatcg ccaccttcct
       61 ctgcattctg ttcgctgcct gtggcggcga tgccgatcgc ggcagcggtt acgcactcga
      121 ccggggactc ggggcggaac cggagtcgct cgattatcac aaggcccgga gcagccaggc
      181 gggcgacgtg caacgcgacc tcggggaagg cctgaccggt tatacgccgg agggtgaact
      241 ggtaccgcga gccgccctgc gctgggaggt atcggacgac gggctcgagt acaggttctg
      301 gctgcggccc gatgcgcgct ggtcaacggg cgagccggtg acggctgagg attttgtgta
      361 cagcttccgg cgcctcgtcg ctccggcaac ggcatcgttt tactcccagt cgatcatcga
      421 cgtgaggaat gcggaagcga ttctcgccgg cgagaaagca gtggaggaac tggctgcaac
      481 cgcggaaggg gagttcctgt tgcatttggg ccttgcgcgc ccggcgccat atttcctggc
      541 actgctcacg cacccgagca tgttccccgt gcaccgcgcc tcggtggaac ggcatggcga
      601 tgggttcgcg agaccgggca acctcgtcac caacggcgcg tacaggcttg tcgagtggga
      661 gcttggctcc ctgatcgagc ttcaacgcaa tgagaactac tgggacaatg catcgacggc
      721 gatcgatcgt gtacgccatc atttcgtggc ggagccggca gttgaactga atcgctatcg
      781 cgccggggaa ctgcacatca ccagtacggt gccgcccgag gcattcgcgc gaatgcgcga
      841 gacccgcccg gaggagcttc gcgttgctcc ttatctcaac gtttactact acggcttcaa
      901 cctcacgaag ccgcctttca aggacaaccc ggagctgcgg cgcgcgctgt cgatggcgat
      961 agatcgcgag gcgctgaccg agagtgtcac gggacgcggc gaaactcctg cctggagctg
     1021 ggtgccaccg ggcgtcgaca actacagcga acgccggttt ccatattcga cgatgagtga
     1081 tgaggaacgc gaggctgcgg cgaagcgagc ctatcgcagc gcaggctacg gcgacggcga
     1141 gccgatcgag atcgaaatac gctacaacac ctccgatacg caccagcgca tcgcgctggc
     1201 catacaatcg atgtggcgcg acgtgctggg ggtcgaggcg acgctggtaa acgaggagtt
     1261 ccaggtgttg ctgtcgaaca tgcgagcgaa ggaggtgacg caggtttttc gctcgagctg
     1321 gctcggcgac tacaacgatg cgaatacgtt cctccaggtg atgctgtccg atgccccgtc
     1381 gaacctgacc gggtacgcca acccggattt tgacgccctg gttgcgcaag ctgccgcgca
     1441 gacggacccc gaagaaaggc ggcgctacat gcaggaagct gaaagcctcc tgttggccga
     1501 tcatcccgtc atccccattt actttttcgt gagcaagcac ctggtcagtc cgcggatcgg
     1561 aggctgggga gacaatgtcc tggattatca ttacagtcag cacctgagcc ttttacccga
     1621 ataagaatca ccggaccccc ggatgctgcg tttcgcgttg ctgcgaatac tgagtgcaat
     1681 accgacgtta ttcctggtcg tggcgctcgc cttcctcatg gtgcacgcag cgccgggcgg
     1741 accgttcgac caggagcgtg tgctgccacc ggataacgcc aggaacctgg cggcggccta
     1801 tcacctcgac gaacctttgc ctcgccagtt cgttcgttat gtatcgggcc tggtgcgcgg
     1861 cgacctgggt ccctcttatt attatcgcga ccacgacgtt tccgacctca tcggtgcagc
     1921 gttgccgctg tcactgcaac tcgggctcct gtcgatggcg gtggcgctcg tcgccggcat
     1981 attcgccggc attgccgcgg cactgaaaca agacacgttg ctggaccggt tcgtgacggg
     2041 tttggccatg accggtatct cggtacccgt attcgtcgtt gcgccattgc ttgtgctcgt
     2101 cttctcagtg caactcggct ggcttccggc gagctggagc ggcagtgccg gctggagtcg
     2161 attgctgctt ccggttctcg ccttgtcgct gccccagatt gcttacgtaa cccgcctgac
     2221 gcgggccagc atgatcgagg tgctgagcag cgatttcatc aggaccgcga gagcgcaggg
     2281 cctctccacg gtgtcgatca tccgttacca cgcactgaag ccagcgatgc tgccggtggt
     2341 gtcatacctc gggccggcga cggccgcggt attgaccggc gccgtggtgg tcgagatcgt
     2401 gttcggtgtg ccggggctgg ggcagctctt cgtacgcagc gcgctcaacc gggactacac
     2461 gctcgtgctg ggaatcgtca tcttctacgc ggcccttatc atcctgctga acctgctcgt
     2521 cgacctgctg tacggcatcc tcgatccgcg aattcgcgaa tcatgaatgc agtacccgca
     2581 gcagtagccg gtgcagctga tcggcttgag tacggcaggc tggtgagggt tccggcagcc
     2641 atactgctgc tgttgcttgc cgctgccctg ctcggtccct ccttgagtcc gcacgaccac
     2701 ttcaccatgg atctgtccac gcgcct
//
LOCUS       DASUOM010000340         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1798787,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000340 DASUOM010000000
VERSION     DASUOM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798787"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..730)
                     /gene="rpsI"
                     /locus_tag="VFY27_07185"
     CDS             complement(338..730)
                     /gene="rpsI"
                     /locus_tag="VFY27_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5765334.1"
                     /translation="MMNEQFYGTGRRKTSTARVFLKPGSGAITVNDKPLDRFFGRETA
                     RMIVRQPLKVMDLNERFDVNVTVSGGGTTGQAGAIRHGLTRALMKYDESFRQKLRKAG
                     FVTRDAREVERKKVGLRKARRATQFSKR"
     gene            complement(778..1212)
                     /gene="rplM"
                     /locus_tag="VFY27_07190"
     CDS             complement(778..1212)
                     /gene="rplM"
                     /locus_tag="VFY27_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5765335.1"
                     /translation="MKTFSAKSEEIERDWYVVDATGKTLGRLSTEIARRLRGKHKPQY
                     TPHVDTGDYIVVVNASKIRVTGNKLKDKMYHRYTGYVGNLKSIPLQKLLDDAPERALQ
                     FAVKGMLPRNPLGRRMFSKLRVFPGPEHTHEAQQPKHLEINA"
     gene            complement(1481..>2120)
                     /locus_tag="VFY27_07195"
     CDS             complement(1481..>2120)
                     /locus_tag="VFY27_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX5765336.1"
                     /translation="EPWSGKAALGYLATSGNTESTSLNSGFTLAYATGRWAHSLEASA
                     IKASEDDATSAEAYSVGWKTEYNMTERDFLFGRVNWRKDRFTAYEQQLSESVGYGRRL
                     IDTGVHFLNVELGAGARQADLVDGTAEEDFIFRGGIAYQWKFSETAAFTQDINAESGP
                     GNVYTESVSAITTKLMGNLGLVASYTIKNSSVVPPGSENSDTYTALSLEYAF"
BASE COUNT          368 a          663 c          623 g          466 t
ORIGIN      
        1 gctctattgt accgaccagg atagacgagg cccatccatc ggcctcgccc tccgggcgac
       61 gtcgcttcgc gccgtcgtcc aaatttgttc ccgacaaatt tgtcgaacct tgagttggcg
      121 gagtcagagt ccgcagtcct accgccagac gatcccccat ccgaggacga ctttgtactt
      181 caggagttgt aggagcccgc catgcgggcg agttcgatat gcttcagcgg aaaccttccg
      241 agtcggatgt ccggagattt cggtatgcct aagctcctgc agttgccaat cgatcacgct
      301 gaacagcaag cagcctgccg gctgctgctc aatccaacta gcgcttcgag aactgggtag
      361 cgcggcgcgc ctttcgcagg ccgactttct ttcgctcgac ctcgcgcgcg tcgcgggtta
      421 cgaatcccgc cttgcgaagc ttctggcgga aggactcgtc gtacttcatc agggcgcgcg
      481 tcagcccgtg gcgaatggcg ccggcctgcc cggtcgtgcc gccgccgctg accgtgacat
      541 tcacgtcaaa gcgctcattc aggtccatga ccttcagggg ttgccgaacg atcatcctcg
      601 ccgtctcgcg gccgaagaac ctgtcgagcg gcttgtcatt gacggtgatc gcaccgctgc
      661 ccggcttcag gaatacccgg gcggttgagg tcttgcgccg gccggtgccg tagaactgct
      721 cattcattat atatggctcg gttgtctatc tgtctgtccg tcttggccgt ccgaccatca
      781 ggcgttgatt tccaaatgct tcggctgctg cgcctcatgg gtgtgctcgg ggccggggaa
      841 tacgcgcagc ttcgaaaaca tcctccgtcc cagtgggttg cgcggcagca tgcccttgac
      901 ggcgaactga agggccctct ccggcgcgtc atccagcagc ttctggagcg gtatcgactt
      961 caggttgccg acatagcccg tgtaacggtg atacatcttg tccttgagct tgttgccggt
     1021 cacgcgaatc ttgctcgcgt tgacgaccac gatgtagtcg cccgtgtcga cgtgcggcgt
     1081 gtactggggc ttgtgcttgc ccctcaggcg cctggcaatc tcggtcgaca ggcgccccag
     1141 ggtcttgccc gtggcgtcca cgacgtacca gtcgcgctcg atttcttccg acttggcgga
     1201 aaacgtcttc atgattccag gtatccagtt gggattggcc cccgggtgcg gggcatttga
     1261 ggatccgtac ggcctcgagc gcccggacgc gccagatgct cgtccagtcc cggcgaaagg
     1321 cgcgaaattc tactcgacgc accggcccaa tgcaaacagc ggctgatgca gtcgcggcct
     1381 gcgcatggct cgggcgaaca gctcattaat cctttctccc tcagctactt atttagcggc
     1441 tttatagaag ggaatattaa cccggccggt ctcgccgcag tcagaacgcg tactcgagcg
     1501 acagagccgt ataggtatcg ctgttttcac tccccggggg aaccacgctg ctgttcttga
     1561 tggtatagga cgccaccaag cccaggttgc ccatgagctt ggtcgttatt gcagagaccg
     1621 actcggtata gacgttaccg ggtcccgact cggcatttat gtcctgggtg aaggcagccg
     1681 tttcactgaa tttccactgg taggcgatgc cgccgcggaa gatgaagtct tcctcggcag
     1741 tgccatcgac gagatccgcc tgacgtgcac cggcaccgag ctcgacgttc aggaagtgca
     1801 cgccggtatc gatgagccgc cggccgtaac caaccgactc ggacagttgt tgttcatagg
     1861 cggtgaagcg gtccttccgc cagttcaccc ggccgaacag gaagtcacgc tccgtcatgt
     1921 tgtattcggt cttccagccg actgaatagg cctcggcgct ggtggcgtcg tcttccgagg
     1981 ccttgatcgc gctcgcttcc agggaatgcg cccagcgtcc cgtcgcatac gcaagcgtga
     2041 accccgaatt caggctcgta ctttcagtat tgccgctggt cgcgagatag ccgagtgcag
     2101 ccttgcctga ccacggctcc
//
LOCUS       DASUOM010000341        11137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1118045,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000341 DASUOM010000000
VERSION     DASUOM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118045"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFY27_07200"
     CDS             complement(<1..590)
                     /locus_tag="VFY27_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5765337.1"
                     /translation="MRHTEIAVRRPVTTLVVFVALALVGLIASRLLPLEKFPDIEFPG
                     IFIQVPYEGSTPEEVERLITRPIEEALATLSGVETMVSTSSESQAEVFLEFGWGQSMG
                     AKGIEARAKVDGIRHLLPEDVRRVFIFTGSLGDQPVLQLRISSQRDLSGSYELLDRLL
                     KRRIERVEGVSRVDLQGVDPKEIRILIDADRMSAHGV"
     gene            complement(595..3819)
                     /locus_tag="VFY27_07205"
     CDS             complement(595..3819)
                     /locus_tag="VFY27_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5765338.1"
                     /translation="MRRLIEIATERRVTIVMFTIAIALFGMVSLSRLNLTLLPDISYP
                     TITIRTELTGAAPIEVENLLTKPIEEAVGVIRNVRLVRSVSRAGQSDVTLEFAWGTDM
                     DIAGVDVREKVDIIELPLEAERPLLLRFDPSSEPIVRLGLLQKGAGAEVGGASEGQLK
                     ELRRLAEDRIKNDLEAEEGTAAVKVSGGLEDEIQIQVDQQKLSQLGISIELIAERIRA
                     ENVNLSGGRLEEGAQRFLVRTLNEFQTVEEFANAIVANVADRPVYLRDVATVSRGYKE
                     REAITRVNGKESVELAVYKEGDANTVQVARRIDRRLEQVRETLPEDLELVKIYDQSKF
                     ISAAIQQVTSAAISGGFLAILVLYGFLRDAKATSIIALAIPVSVIGTFLLMYTNDVSL
                     NIMSLGGIALAVGMLVDNSIVVLENIVRKREQGQGDLEAARNGTSEVSGAVIASTLTT
                     IAVFFPMVFISGIAGQLFRDQALTVTFALMFSLLVALTLIPMLAALGSRSRYVEAGEE
                     SAPNRFTRGVAFIVRLFAYGFAAVRWVFWTLLWIPSWVLQRLNSAAANRYAPLLEWSL
                     RHRGAVVAGASLIFVASMLLIPKLGTELIPQLSQGEFNVDLRLAPGAPLFETDRAIQT
                     AEQATKDIDIIALNYSVAGTGNRLDASPVDAGENTGTLSVTLEAGAGREAEQSAMNVM
                     REALSHLPGVQYEFSRPSLMSFSNPLQIEISGYDLLALEAVNRDIVGALAQSARFTDI
                     RTTIEMGNPEIQIVFDQERAAALGLSVRDIADRVVANVRGELATRYTWRDKKIDVLVR
                     SVDRENASIGDVRSLIVNPSSERPVTLEAVAAVGVARGPSEIRRVAQERVAIITANLA
                     FGDLGAAAAEAHDIINRTPMPSGVTAMVSGQNEEMQESFRSMQFALILAVFLVYLVMA
                     SQFESLVHPFVILFTIPLALVGAVLGLYVTGTTVNVVAFIGVIMLAGIVVNNAIVLVD
                     LINQMRAQGMEKSAAILMAGRTRLRPILMTTLTTVLGLLPMAIGFGEGAEVRAPMAIT
                     VIGGLLTSTILTLIVIPVVYSLLDRKTYAMLQSTAGELPAPARS"
     gene            complement(3840..4943)
                     /locus_tag="VFY27_07210"
     CDS             complement(3840..4943)
                     /locus_tag="VFY27_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5765339.1"
                     /translation="MEITIFPWRQRTALLVLSAVLVACQEGAGEKDAEKEEAPAIPVE
                     ITAPSRGDIYAVYSGTAPIEAFADAQVTAKVGGEVREIYVEEGDDVVAGEVLLRLDGD
                     RLRFEAQQAEANLLKLQRDFQRNVDLKDRSLISAGDFEKIQYEMEALQATASLAKLEL
                     SYTEIRAPIDGVVSQRYVKVGNTIEVNSPTFQITSLEPLVSYLHVPEREYRRIEPGQT
                     AAIAVDALQGVRFEGTVARVSPVVDPLTGTFKITIEVSDLSRSLKPGMFGRISIVYDK
                     HENALQIPRNAIIQEAGEPAVFVVDGDTAHRRPVSTGYVQGSRVEILDGLADGERLVL
                     VGQTGLKDGSKVSVINADDADISAASSNVPASE"
     gene            complement(5004..6074)
                     /locus_tag="VFY27_07215"
     CDS             complement(5004..6074)
                     /locus_tag="VFY27_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5765340.1"
                     /translation="MPNKSIISLLCTLCAAALPGCGAGEASVAVAETEALMPVAVALP
                     ETMDIYETLETTASIESDAEAAVLARASGEVVEIRVEEGDRVEEGQVLALLDGERLRL
                     QMLQAKASLDMTSREYERFVNLRQRGLVSASAVEGLKFDVDALRASYELMRLNYAYTK
                     VRAPISGVISSRDVKIGTHVDPGAPLFSITETSRLVAYLHIPQVELSRISTGDEVLVQ
                     VDAMPGHEFRARLARISPTIDTRNGTFRATVYIVNTQGLLVPGMFSRFGISFEEHADA
                     LVVPTRALVEEDGTHVVYVVEDGAAARRIVQVGIASGGVTEILAGLDAGESVVVTGQA
                     SLRDGSKVLASLSGDASSSGTG"
     gene            complement(6568..7932)
                     /locus_tag="VFY27_07220"
     CDS             complement(6568..7932)
                     /locus_tag="VFY27_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5765341.1"
                     /translation="MSVRAATLRDTGKIGRRTVPRPCTIRPTSLKQLRQCLEQGSSYL
                     APFRPMGANSSSTDCTSATAGTVIDMTAFDEIVNIDAYNDTAVVQAGVRLGRLANALA
                     EHGLELAGSHDFMSRTVGGAVAGGCIGPSISSDGAYFASQVLSMKLVTPSGRLLELSR
                     EKQNLLNAFRLSYGMLGVIYEVTLKVRPVSPFAVSHRRCSIAQFSAAAENLSRTDIGI
                     KFYLLPFRDCAYLDLRRYSREVTSTHKIPWKFKDWGESTVLPQVFKSLNRIVPVAGVR
                     YRIIDEISKVTQNIVNNRLVTSGSNSTALANGDHANHGEPLRYSTWMFPAADFSIVIQ
                     AYQDFCQRIRKESGFRCDMPTVGFRLCRDQSALLSPSFDEPMFVLRAVSTQARGWEDF
                     AIDFGEFAQHWGGMPFFNQTRGLSPGYAVQVFGSRLDFFKRMRRRIDPENRMMNPFLL
                     QYFL"
     regulatory      complement(8105..8188)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8367..9095
                     /locus_tag="VFY27_07225"
     CDS             8367..9095
                     /locus_tag="VFY27_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5765342.1"
                     /translation="MKTFEPVRRQAALAATILVTAMALAACTDQHNTFAETRSITVTG
                     EGEASGAPDQASVSAGVQILAESVIEATHENESIIGKIMEALDEQGIPPEKIQTSNYS
                     IWAEQNYQEPGQNRISGYHVSNVVTVKIDDVDKLGDVLGAVVNAGANSIHGIQFGVKD
                     TKALEQQAREAAMADARARAESLARLAGVQLGEVLSISTSYGGGPPRPMMSRSYDMAE
                     AAAPSISPGQQSLSVQIHATFAIR"
     gene            9270..>11137
                     /locus_tag="VFY27_07230"
     CDS             9270..>11137
                     /locus_tag="VFY27_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5765343.1"
                     /translation="MTSCLGALARATMAVLLMVSTAAAEEITWVITGVPEPLLANVRS
                     HVEDVGFGTMARVSAGQFDEITKNARLRAREALKPLGYYHPVISTSVAPAGRERYRIN
                     LRIEPGPPVRVKQAHIALRGDGERQEDLREWRQEWPLKEGSILNQATWEEQKQAALSL
                     AHAEGYVNADFVTRTIELDLARNEANLTLVLDTGVQAVFGTMDFVQDVVEPYALANVP
                     RFAPGTPYRPDLVESLRLDLWTTGYFTDIEVEEQRQLDRTPPTVNIVASMETEWKNTY
                     QGALGFGTDTGFRTQALWSRRPLSSRGDRLDVGIGYQEMDDQFTMRADYRIPRRVEKR
                     QFWIANMLLRRDKQDLEVKRNEDDEDFIQLATGNVDEVFLRAGRLYVRDIDEGKVRYF
                     ETMFAQYLRESYSYDPGADADPAVLDLLQDPEFGRIFRDTVETVALGVEWDWPAVTGS
                     GFDITGHRERAWIFTSSDLWGSDEDFTQLYVSSQRNFIRGDRWKLLLRAEVGYTDAKV
                     DRLTLDIDNEPLALSLTRLPNEYRFKAGGSNSVRGYGFEELSNNDIGSNHIITASAEV
                     EMRVFEKWSAAAFVDIGNAFNDWNDFELRRGAGIGIRWYTVAGPLRLDVAQALDVEG"
BASE COUNT         2180 a         3389 c         3400 g         2168 t
ORIGIN      
        1 acgccatgcg ccgacattcg gtcggcgtct atcagtatgc ggatttcctt gggatcgact
       61 ccctgcaggt cgacacggga tacgccctcg acgcgttcga tgcggcgttt cagcagccgg
      121 tccagcaact cgtaactgcc ggagaggtcg cgctggctgg aaatacgcaa ttgcagcacc
      181 ggctggtcgc caagcgagcc cgtgaagatg aaaacgcgcc gcacgtcttc cggaagaagg
      241 tggcgtatgc cgtcgacctt ggcgcgcgct tcgatgcctt tcgcgcccat gctctggccc
      301 catccgaact ccaggaagac ctccgcctgg ctttcgctcg atgtcgatac cattgtctcg
      361 acgccactca aggtagcgag ggcttcttcg atgggacgcg tgatcagcct ttcaacctct
      421 tccggcgttg atccttcata aggcacctgt atgaatattc ccggaaattc gatgtccggg
      481 aatttttcga ggggcagaag ccgggacgcg ataaggccga cgagcgcgag cgccacgaac
      541 accaccagcg tggtgacagg ccgccgcacc gcaatctcgg tatgtctcac ggcttcagct
      601 tcgagccggt gcggggagtt cgccggcggt cgactgaagc atcgcatacg tcttgcggtc
      661 gagcagcgag tacaccaccg ggatcacaat cagcgtcaag atcgtggaag tcagcagccc
      721 cccgatgacc gttatcgcca tcggcgcgcg tacctccgcg ccctcgccga agccgatcgc
      781 catcggcagc aggccgagca cggttgtcag cgtcgtcatg agtatcgggc gaagacgggt
      841 tcggccggcc atcagtattg ccgccgattt ttccatgcct tgggcacgca tctggttgat
      901 caggtccacc agcacgatcg cgttgttcac gacaattccg gcgagcatga tcacccctat
      961 gaaggcgacg acgtttaccg tggtgccggt tacgtatagc ccgagtaccg cgcccacgag
     1021 ggccagcgga atggtgaaga ggatgacgaa cgggtgaacc agagactcga actgcgatgc
     1081 catcacgaga tagacgagga agaccgcaag tatcagcgcg aactgcatcg agcggaacga
     1141 ctcctgcatt tcctcgttct gcccggatac cattgcagtc actccggaag gcatgggagt
     1201 gcggttgatg atgtcatgcg cttccgccgc ggcggcgccg agatctccga aagcgagatt
     1261 ggctgtaatg atcgccaccc gttcctgcgc aacccggcgt atctccgacg gcccccgcgc
     1321 gacgccgaca gcggcaacgg cttccagggt gaccggccgc tccgacgacg gattgacgat
     1381 caggctgcgt acgtccccga tacttgcgtt ttcgcgatcg acgctgcgca ccagcacgtc
     1441 tattttcttg tcgcgccacg tgtaacgagt cgcaagttcg ccccgcacgt tggcgacgac
     1501 gcggtctgca atatccctga cagacaatcc cagtgcggcg gcacgctcct ggtcaaacac
     1561 gatctggatt tccggattgc ccatctcgat cgtcgtccgg atgtccgtga aacgcgccga
     1621 ctgcgccagt gcaccgacga tatcgcggtt taccgcttcg agtgcgagga ggtcgtagcc
     1681 tgatatctcg atctgcagcg ggtttgaaaa actcatcagc gagggccggc tgaactcgta
     1741 ctggacgcca ggcaggtgcg agagagcttc gcgcatcacg ttcatggccg actgctcggc
     1801 ctcgcggcct gcgcctgcct cgagcgtgac gctcagggtg cccgtgtttt cgccggcatc
     1861 taccgggctg gcgtccagcc tgttgccggt gccggcaacc gagtagttga gcgcaatgat
     1921 gtcgatgtcc ttcgtcgcct gctcggccgt ctggatggcg cgatccgttt cgaagagggg
     1981 cgcgcccggc gccagccgca ggtcgacgtt gaattctcct tgcgacagtt gcgggatcaa
     2041 ttcggtgccg agcttcggga tgagtagcat tgatgcgacg aaaatcagcg acgcgccggc
     2101 aaccacggcc cccctgtgcc tgagcgacca ttccaggagg ggcgcgtaac gatttgcagc
     2161 cgcgctgttc agtctctgga gaacccagga aggtatccag agcagcgtcc agaataccca
     2221 acgcacggcg gcaaagccgt atgcgaacag gcgcacaatg aaagcgacac cccgcgtgaa
     2281 gcgattgggg gcggattctt caccggcttc tacgtatcgg ctgcggctgc cgagggctgc
     2341 cagcatcggt atcagggtca gtgccaccag cagcgaaaac atcagggcaa aggtgacggt
     2401 caatgcctgg tcgcggaaga gctgccccgc gatgccgctg atgaaaacca tggggaagaa
     2461 gacggcgatc gtggtcaggg tagaagcgat cacggcgccc gagacttcgc ttgtgccgtt
     2521 ccttgccgct tccaggtcac cctggccctg ctccctcttg cggacgatgt tttccagcac
     2581 gacgatcgag ttgtcgacca gcatgccgac ggccagtgct ataccgccga gggacatgat
     2641 gttgagggac acgtcgttcg tatacatcag gaggaaggtg ccgattaccg acaccggaat
     2701 tgcgagggcg atgatggagg tcgcctttgc atcgcggagg aacccgtaaa gcaccaggat
     2761 cgcgagaaag ccgccggaga tcgcggcgga ggttacctgc tgtatcgccg cggaaatgaa
     2821 cttcgactgg tcgtaaatct ttaccagttc gagatcctcc ggaagggtct cccggacttg
     2881 ctccagccgc cggtcgatcc gcctggcaac ctgtaccgtg ttcgcatcgc cttccttgta
     2941 gaccgcgagt tcaacggact ccttgccgtt cacgcgagta atcgcttcgc gttccttgta
     3001 accgcggctg accgttgcta cgtctctcag gtaaacaggg cggtcggcaa cattcgcgac
     3061 aattgcgttt gcgaactcct cgacggtctg gaactcgttg agcgtgcgca ccaggaatcg
     3121 ctgcgcgcct tcttcgagcc ttcccccgga caggttgacg ttctcggcgc gaattcgctc
     3181 cgcgatgagt tcgatgctta tgcctagctg cgacagcttc tgctggtcca cctggatctg
     3241 gatctcatct tcgaggccgc cactgacctt gaccgcggcc gtaccttcct cggcttccag
     3301 gtcgttcttt atcctgtcct ccgccagcct gcgcagctcc ttaagttgtc cttcgctggc
     3361 gccgcccacc tcggcgccgg cacccttctg cagcaggccc agccggacga taggctcgga
     3421 ggaaggatcg aaacgcagca ggagcgggcg ctccgcttcg agcggcaatt cgatgatgtc
     3481 gactttctcg cgaacgtcga caccggcgat atccatgtcc gtgccccagg cgaattccag
     3541 cgtgacgtcc gactgcccgg cacgcgaaac cgaccgtacc agccgcacgt tccttatgac
     3601 gccgacagct tcctcgatcg gcttggtcag caggttttcc acctcgatcg gcgccgctcc
     3661 ggtgagttcc gtgcgaatgg tgatggtggg ataggagatg tccggcaaca gcgtcaggtt
     3721 gagccgtgac aaagacacca ttccgaaaag tgcgatcgct atcgtgaaca tcacgatcgt
     3781 cacgcgacgt tccgtggcaa tttctatgag tcgacgcatg ttcgcgttct gtccctggct
     3841 tattcggaag ccggcacgtt actgctggca gcgctgatat ctgcatcgtc ggcatttatg
     3901 accgacactt tcgaaccgtc tttcaggccc gtctgcccta cgaggaccag gcgctccccg
     3961 tcggcaaggc catcgagtat ttctacgcgg ctgccctgga cgtagccggt gctaaccggc
     4021 cgccggtgag ccgtatcgcc gtccacaacg aacaccgctg gctctcccgc ttcctggata
     4081 attgcgttgc gcgggatctg cagtgcgttc tcatgcttgt cgtacacgat gctgatccgg
     4141 ccgaacattc ccggcttcaa gctgcggctc aaatcggaca cttcgatcgt aatcttgaaa
     4201 gtgcccgtca aagggtctac gacggggctg acgcgtgcga ccgtgccttc gaagcggact
     4261 ccctgcaaag cgtctactgc gattgccgcg gtctgccccg gctcgatgcg gcggtactcc
     4321 cgctcgggca catgcaggta ggacacgagg ggctcaaggc tcgtgatctg gaaggtgggc
     4381 gaattaacct cgatcgtgtt gcccaccttc acgtagcgtt gcgaaaccac cccgtctata
     4441 ggcgcgcgga tctccgtata actgagctcg agttttgcga ggctggcagt tgcctgcagc
     4501 gcttccatct cgtactggat cttttcgaag tcgccggcgc ttatgagact gcgatctttc
     4561 aggtccacgt tgcgctggaa atcacgctgc agcttgagaa gatttgcttc ggcctgttgc
     4621 gcttcgaagc gcagccggtc gccgtcgagg cgcaggagga cctcccccgc gaccacgtcg
     4681 tcgccttcct caacgtagat ctcgcgaacc tcgccgccca cttttgctgt cacctgggcg
     4741 tcggcgaaag cttctatcgg tgcggtcccg gagtaaacgg cgtagatgtc gccgcgcgac
     4801 ggtgccgtga tttcaacggg gatcgcgggg gcttcctcct tctcggcatc cttttcgccc
     4861 gcaccttcct ggcaggcaac gagtaccgcc gatagcacca gcaaggccgt ccgttgacgc
     4921 cacggaaaga tagttatctc catctctgaa acagcgctcc ccgaaattgg tcgagaggag
     4981 agcaacacct gctctccatc atgctagccc gtgccgctgc tgcttgcgtc accggaaagg
     5041 ctcgcaagga ccttgctgcc gtcgcgcagg ctcgcctggc cggtcaccac gacgctctcg
     5101 cccgcatcga ggcctgccag gatctccgtc acgcctcccg aagcgatccc gacctgtacg
     5161 atacgacggg cagccgcacc atcctccacg acgtacacca cgtgtgtacc gtcttcctcg
     5221 acgagcgcgc gggttggtac caccagcgca tccgcgtgct cctcgaaact gatgccgaag
     5281 cggctgaaca tgcccgggac aagaagcccc tgcgtgttga cgatgtaaac ggtagcgcgg
     5341 aaagtgccgt tgcgcgtgtc gatcgtgggg ctgatacgcg caagcctcgc cctgaactcg
     5401 tggcccggca tcgcgtccac ctgtacgagg acttcgtcgc cggtgctgat ccttgaaagc
     5461 tcgacctgcg gtatgtgcag gtacgctacc agtcgtgatg tctcggtgat gctgaagaga
     5521 ggtgcgccgg gatcgacgtg agtgccaatt ttgacatccc ggctcgatat tacgcccgat
     5581 atcggtgccc gtaccttcgt ataggcgtag ttcaggcgca tgagttcgta ggaggcgcgc
     5641 agcgcatcca cgtcgaactt gaggccttct acggccgacg cgctgacgag tccgcgttgc
     5701 cgcaaattca cgaagcgctc gtattccctc gaagtcatgt ccaggctggc tttggcctgc
     5761 agcatctgca gccggagccg ctccccgtcc aggagcgcca gcacctgtcc ttcctcgacc
     5821 cggtcccctt cctccacccg gatctcgaca acctcgcccg aggcgcgcgc caggacggcc
     5881 gcttctgcat cggactcgat gctcgccgtc gtctccagtg tttcatagat gtccatggtt
     5941 tcgggcagtg cgaccgcgac cggcatcagc gcttccgtct ctgctaccgc aacactggct
     6001 tcgccggctc cgcaaccggg gagagccgcg gcgcaaagcg tgcagagcaa ggatatgatt
     6061 gatttattag gcatttttct acactcgagg cggcagggac gaggcatccc gcagctgcca
     6121 ggcccttgat ccagagtact agtgatagtg ttatagttga gtataacact agataaccat
     6181 aacacaagcc cggcagcacc ctggaaaggg acgcaggaga ccccggtttc gcaatcaccc
     6241 agttagatgc gcgaggcggg cttttggatt gatcttgccc ggctcgctat atgcgggctt
     6301 cggcatcccg atccagtccc gaccgagggc ttgccgatcg agatctacac ggtcaccaac
     6361 acgacggcgt gggtggaata tgaaggcgta cagagcgata tcttcgacca catcctgtcg
     6421 atcgtcggcg agcctgcacg tgtaccagaa gccgagcggg ccggatgttc gtaccgcggt
     6481 agcggcaatg cgcggaacgg aagctgttcg aaaaccggcg gcgttcgctc agtcctcatg
     6541 agcctgcgcc gcggcacccg gcgttggtca gagaaaatat tgcagcagga atggattcat
     6601 catcctgttt tccggatcaa tacgccgccg catgcgcttg aaaaaatcga gcctgctgcc
     6661 gaacacctgg acggcgtacc cgggagacag gcctcgcgtc tggttgaaga agggcatgcc
     6721 gccccagtgc tgcgcaaact cgccgaagtc aatggcgaag tcctcccagc cccgcgcctg
     6781 cgtggacacg gctcgcagta cgaacatcgg ttcgtcgaat gatggagaca acagggcgga
     6841 ctggtcgcgg cacaacctga atcccaccgt aggcatgtcg cagcggaacc cggactcttt
     6901 ccggatacgc tggcagaaat cctggtaggc ctggatgacg attgaaaagt ccgctgcagg
     6961 aaacatccac gtggaatacc ggagtggctc accatggttc gcgtgatcgc cgttcgcgag
     7021 tgctgtggaa ttgctgcctg acgtcaccaa ccggttgttg acgatgttct gcgtcacctt
     7081 gctgatttcg tcgataatgc ggtagcgcac tcctgcgacg ggaacaatgc ggttcaacga
     7141 tttgaagact tgcgggagca ctgtgctctc gccccagtcc ttgaacttcc aggggatctt
     7201 gtgcgtcgat gtgacttccc tgctgtagcg cctgaggtcg aggtatgcac aatcgcggaa
     7261 cggcaagagg tagaacttta tgccgatatc ggttcgggaa aggttctctg ccgcagcgga
     7321 aaattgcgcg atggagcaac gccggtgcga aaccgcaaat gggctgacag gccgcacctt
     7381 gagcgtcacc tcgtagatca ccccgagcat tccgtaactg aggcgaaacg cattcagcag
     7441 attctgcttt tcgcgggaaa gctcgagcag gcggccggac ggggtgacga gcttcatgct
     7501 cagcacctgc gatgcgaaat aggcgccgtc gctggaaata gacgggccga tgcagccacc
     7561 ggcgacagct ccgccgaccg tccggctcat gaagtcgtgg cttccggcca gctcgagacc
     7621 atgctcggca agagcgtttg cgagcctccc caggcgaacc cccgcctgga cgacagcggt
     7681 atcgttgtat gcgtctatgt tgacgatctc gtcgaaggcc gtcatgtcga tgaccgtgcc
     7741 ggcggtcgcg gatgtgcagt ccgtggatga gctgttcgcc cccatcgggc gaaacggcgc
     7801 aaggtatgag gagccttgct cgagacactg tctcaattgt ttcaagctgg ttgggcggat
     7861 cgtgcacggc ctcggcacgg tgcgccggcc gattttcccg gtgtcgcgaa gtgttgcggc
     7921 tctaactgac atgctgctct cccatctgca ccgacgcgta cgaatgccgc gtccggccaa
     7981 ggacacgcgc gcagttgccg gttgggagtg atgcggcaga ctctctggtc tggcccgcta
     8041 tcgcgaatga ggcggattgc ctgcgaacgg gcttacaaga tgctcgtcgc gttgtacctg
     8101 tcgtgtcggc aaccccgctg tcataggata ttcccttaga ccggtggctt tgcggccctt
     8161 cctttcgaaa ggtgtgcctt ttccaaacga agaagaatcg ttgcacagcg ccttcggcaa
     8221 tgtcaatcga cgaaacggat ttacccggaa attgtcaaat cgcgttttgg ggaagcgctt
     8281 cgaacatcga ctacaatgcg cgctcttccg gagagtctca accaggcggt gcgccgggcg
     8341 gcgcttcaac aaaaggagct gtgcacgtga aaacattcga accggtccgg cgacaggcag
     8401 cgctggctgc aaccatcctg gtaacggcga tggctttagc tgcttgcact gatcagcaca
     8461 atactttcgc ggagacgcga agcatcaccg tcacgggcga gggtgaagct tcgggcgcgc
     8521 cggaccaggc ttccgtaagc gcgggtgtgc agatcctcgc cgagtccgta atagaagcga
     8581 cgcacgagaa tgagtcgatc atcgggaaaa tcatggaagc gctggatgaa cagggcattc
     8641 cgccggaaaa gatccagacg tccaactaca gcatctgggc agagcagaat taccaggagc
     8701 cggggcagaa tcgcatcagc ggctaccacg tgtcgaacgt cgtcaccgtc aagatcgacg
     8761 acgtcgacaa gctcggagat gtgctcggcg cagtagtcaa cgcaggcgca aattccattc
     8821 acggcatcca gttcggcgtc aaggacacga aggccctgga acaacaagcg cgtgaggcag
     8881 cgatggctga cgcgcgggcg cgtgcggagt ctttggcacg tctcgcggga gtgcaactcg
     8941 gcgaagtcct gtcgatctcc acttcgtacg gtggcggccc gccgcggccg atgatgtcgc
     9001 gcagttacga tatggcggaa gcggctgctc ccagtatctc gcccggtcag caaagtctca
     9061 gcgtgcaaat ccacgcgacg ttcgccattc gctgacaggc gttttagacg ccggcccgtc
     9121 ccgcggagac gctccgggtt agcgtggcag gttttttgca agcatcatgg ccggcaccgc
     9181 agggcgtcgg cagatgcggg cgctgattgt aattcttacc gattcccatg aaagttgatg
     9241 tcgacaccag cacacgttga gttcccggcg tgacctcatg tctcggcgcg ctcgcgcgag
     9301 ccacgatggc tgtgctcctg atggtctcca cagcggcggc cgaggaaatc acctgggtta
     9361 tcacgggtgt cccggagccg ctgctggcaa acgttcgcag ccatgtggag gatgtgggtt
     9421 tcgggacgat ggcccgcgta tccgccgggc agtttgacga aataacgaaa aacgcgcggc
     9481 tgcgtgcccg cgaagcgttg aagccgctcg gttattacca tcccgtcatc tccaccagcg
     9541 tcgcacccgc cggcagggag cgataccgca tcaatctgcg catcgagccc gggccgccgg
     9601 tccgggtgaa gcaggcgcac atcgcactac ggggtgacgg agagaggcag gaggatcttc
     9661 gcgagtggcg acaggaatgg ccgcttaaag agggcagcat tctcaaccag gcgacctggg
     9721 aggagcagaa acaggcggcc ttgtcactgg cgcatgccga gggttatgtg aatgcggact
     9781 tcgtcacccg cacgatcgaa ctggacctgg ccaggaatga agcaaacctg actctcgtac
     9841 tcgataccgg cgtgcaggcg gtcttcggga ccatggactt cgtacaggac gtggtcgagc
     9901 catatgccct ggcgaacgtg ccgcgtttcg ctcccggaac accgtatcgc ccggacctcg
     9961 tggagagcct gcggctggac ctgtggacga ccgggtactt caccgacatc gaagtcgagg
    10021 aacaacgcca gctcgatcga acacctccga cggtgaacat cgtcgccagc atggagacgg
    10081 agtggaagaa cacgtatcag ggtgccttgg gtttcggcac cgacaccggg ttcaggactc
    10141 aggcgctatg gagccgccgc ccgctgtcct cgcgtggaga tcgcctcgac gtcggcatcg
    10201 gttaccagga aatggacgac cagttcacga tgagggccga ttaccggatt ccccggcggg
    10261 tggaaaaaag acagttctgg atcgcgaaca tgctgctcag gagggacaag caggacctcg
    10321 aagtgaagcg taacgaagac gacgaggact tcatccagct cgcaaccggc aacgtcgacg
    10381 aggttttcct tcgcgccggc aggctgtacg tccgcgatat agacgaaggc aaggtgcgct
    10441 acttcgagac aatgttcgcc cagtacttgc gggagtcgta ttcctatgat cccggagcgg
    10501 atgcggaccc tgcggtcctg gacctgttgc aggacccgga attcggacgg attttccggg
    10561 acactgttga aacggtagcg ctcggcgtcg aatgggactg gccggccgtc acgggaagcg
    10621 gtttcgacat cacaggccat cgcgagcggg cgtggatatt cacgtcaagc gacctgtggg
    10681 gctcggacga ggactttacg cagctgtacg tctcatcgca acggaacttc attcggggtg
    10741 accgctggaa gctcctgttg cgcgcggaag tgggatatac ggacgccaag gtagaccgcc
    10801 tgacgctcga catcgacaac gagccgctcg cactttcgct gacccgactg ccgaacgagt
    10861 accggttcaa agccggcggg agcaacagcg tgcgcggcta tggcttcgag gagctgagca
    10921 acaacgacat tggctctaac cacatcatca cggcgagcgc ggaagtcgaa atgcgcgtct
    10981 tcgagaagtg gtcggccgcc gccttcgtag acatcggcaa cgctttcaac gactggaacg
    11041 actttgagct tcgcaggggc gccggcatcg gaatccgctg gtacacggta gccgggccgt
    11101 tgcgcctgga tgttgcgcag gcgctcgacg tcgaggg
//
LOCUS       DASUOM010000342         6325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_991910,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000342 DASUOM010000000
VERSION     DASUOM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991910"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFY27_07235"
     CDS             <1..882
                     /locus_tag="VFY27_07235"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX5765344.1"
                     /translation="PGQLTHDYLIGRRARFMPPFRTYLVLSIVFFLVAFFDPQQQFGI
                     LFEPEPEAQTAEERRRTAEEIRKEVLEDLAAEGIDVPKSSGTPGDVPLPEPPPAPEVE
                     AERSDNGLNVQVTPSGELDVNCTLDSFESDAPEWFTRRVTQERLQEICDRVVADSGRS
                     FLNQLLDNIPAALFILLPLMALVLKMLYPLSKRYYVEHLLFVLHFHAFFFLILTLEVL
                     FSRATSWLGLPDLVRNLSVFVVSVYVPVYLYKAMRRVYEQGRAITLLKYLFLFLTYVI
                     GLFLLLLFAAFYTAFSI"
     gene            1004..2476
                     /locus_tag="VFY27_07240"
     CDS             1004..2476
                     /locus_tag="VFY27_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765345.1"
                     /translation="MSRKTIGISGLAAYVPPYRVWLDDWCEWTGNHWPKIREVVGRSF
                     RVRGPNHSVYTLAATAVMRLIEQYDVDPLRVKFLGLGTESSTDNSAGAIIVKGMVDRA
                     LQSKGKPAIARSCEVPEFKHACLGGVYGMKGALRHLALDGAGSQAIVVCADIAEYARG
                     SSGEPTQGAGAVAMLLEENPRLAVVDLIRSGSASDYRIMDFRKPMLRFCGQDRSESHQ
                     VQDFPVFNGKYSTTCYLDETLHALNDMYEKRGLQATDYLRSLNTVFMHRPYRKMPETG
                     WAVAYLFALSKGTAEDRAELASYCYEAGIEPEALLREMLGKPEIVALAEPERLNFEVY
                     PLTMAVFRIFRASRRYRREILDKLALGSDTMLDLGNLYTAALPAWIAAGFEQALDEGR
                     ELEGEELLTLGYGSGDAAEVIPFFAAGHWREAAASIRFAAAMELAVDLNREQYEALHD
                     GKRVNGLAYIPQNEFIIDKVGHSDQRQFQDIGIEYYRYVG"
     gene            complement(2514..3095)
                     /gene="idi"
                     /locus_tag="VFY27_07245"
     CDS             complement(2514..3095)
                     /gene="idi"
                     /locus_tag="VFY27_07245"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX5765346.1"
                     /translation="MQDHASRIVSSEKEQLILVDENDVEMGHIDKQSAHDAKGLLHRA
                     FSLFIFNPDGKLLMQQRSGDKRLWPNYWSNSCCSHPRRGETMQEATRRRLRDELNIDA
                     ELEFVYKFTYQASYDDLGAENELCWVYLGRTADVIVPNRNEIADTGWLSSSELQRKLE
                     EKPARFTPWFKLEWQRLKADHAQVLGKYLRSGV"
     gene            complement(3104..5383)
                     /locus_tag="VFY27_07250"
     CDS             complement(3104..5383)
                     /locus_tag="VFY27_07250"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEX5765347.1"
                     /translation="MGRPPARDSRIGGLYRMSVSERIDTLTERKWLDAATAARLKSGA
                     SLLSAERADRMIENVIGVFGLPLAVAPNFLVNGRDYVVPMVVEEASVVAAVSGAAKLA
                     RASGGFSVDVGESLLAGQILVTKVADADAAMSAVERAAQELVALANSLQPNLVARGGG
                     ARDVEVFRARLAADGAAAESAGDTYIVLHLLVDTCDAMGANTVNTMCEGIAPRVEELT
                     SGTAVMKILSNLADRSVVSARATFPVSALAEPGHSGQGVRDAIVLAGRFADADAHRAA
                     THNKGILNGIDAVAVATGNDWRAIEAGAHAFAARNGGYRSLTGWTVDLGGNLAGRLEL
                     PLKVGVVGGSMQANPAVAVSLQLTGATTSAELAGLMAAVGLAQNFAALRALVTKGIQE
                     GHMRLHARNVAASAGTPPEHFEAIVERLVESGEIKVRKAADLLAAISAGGESRASESN
                     RAPEVHGRAAGKVILLGEHAAVFGRRALALPLASGVTASVRECTGRTTLEFASDDQPL
                     AQPAVGLDELANLVVGQLGLAGRHFEVRVQSAVPRACGLGSSAAIAVAVIRAFDHVFA
                     LGLSNEAVNDLAYTCEKVAHGNPSGIDNTVATYEKAVLYCKGAAQPMQKLELAEFPPI
                     VIASSGVQSSTKEQVAAVAAKHAATPARYNAIFDEMDRISAEGAAALVRQDYRTLGIL
                     MNLCQGLLNAIGISTPELERMVDIARKHGAAGAKLTGAGGGGCIVALCPGREQQVALA
                     LRDAGYAVIRAGFERDHAV"
     gene            complement(5392..>6325)
                     /locus_tag="VFY27_07255"
     CDS             complement(5392..>6325)
                     /locus_tag="VFY27_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765348.1"
                     /translation="ESTREDCHRVASPGFAAEARFRCTERGSIDWLDEYGNGDRPNAD
                     QVLFECVWRTVNAMPAENLSFRLDTRRFFDAESSLKLGFGSSSALAAALTAVLVRAGP
                     EEATTRPARDIRQLAMRAHLDFQGGKGSGVDVATAVSGGLIAFAVRPDQRIKALKWPP
                     GISYSMMWSGKPASTAERLRKLERFERNRAARAAAAKLVDASRNVLDAWIKQKPGDIF
                     ESLQFYTAALRRFSLEHGVGVFDAGHQELCEAADDLGFLYKPCGAGGGDIGIVFLPGD
                     AKDSSVAEFGIRAGQLGFRKLDRTLEPTGLMYEH"
BASE COUNT         1189 a         2043 c         1861 g         1232 t
ORIGIN      
        1 cccgggcaac tcacgcacga ctacctgatc ggacggcgag cgcgcttcat gcctccgttt
       61 cgcacctacc tcgtgctgag catcgtcttt ttcctggttg cctttttcga tccgcagcag
      121 cagttcggaa tactgttcga acccgagccg gaggcacaaa ccgccgagga gcggcgaaga
      181 acggcggagg agatccggaa ggaagtcctg gaagaccttg ccgccgaagg aatagatgta
      241 cccaagtcca gcggaacgcc aggagatgtt ccgctgccgg agcctccacc ggcaccggag
      301 gtggaggcgg aacgatcgga caacggcctc aacgtgcagg taacgccttc aggcgagctg
      361 gacgtcaact gcaccctgga cagttttgaa tcggatgccc cggaatggtt tacacggcgc
      421 gttacgcagg aacggctcca ggagatctgc gacagagtcg tcgccgacag cggccgttcc
      481 ttcctgaacc agctgctcga caacataccc gcagcgctct tcatcctgtt accgctgatg
      541 gcgctggtgc tcaagatgct gtacccgctg tcgaagcgct actacgtgga gcacctgctg
      601 ttcgtacttc acttccacgc ctttttcttc ctgatcctta cgctcgaggt cctgttttcg
      661 cgcgccacgt cgtggctcgg gctgccggac ctcgtcagga acctctccgt gttcgtcgtt
      721 tccgtctatg tgcccgtgta cctgtacaag gcgatgaggc gggtatacga gcagggccgg
      781 gctatcacgc tgctcaagta cctgttcctg ttcctgacgt acgtaatcgg cctgtttctc
      841 ttgctgttgt ttgcagcgtt ttataccgcg ttctccatct gacggaccga gagaactgct
      901 tgagcgacag gtcgcggaga actagaattt ccgggctcgg catactccgg tcagtttcca
      961 aggcagccac ccgactacgc attaagtagc ccgatacccg ttcatgagca gaaagaccat
     1021 aggaatcagc gggctagccg cttatgtgcc gccctatcgc gtgtggctgg acgactggtg
     1081 cgaatggacg gggaaccact ggccgaaaat tcgcgaggtc gtcgggcgca gctttcgcgt
     1141 acgcggcccg aaccacagcg tttatacctt ggcggccacg gcggtgatgc gtctcatcga
     1201 gcaatacgac gtcgacccgc tgcgcgtgaa attcctaggg ctcgggaccg agtccagtac
     1261 cgacaactcc gccggggcaa tcatcgtcaa gggcatggtg gaccgtgcct tgcagtcgaa
     1321 gggaaaaccg gccatcgccc gcagctgcga ggtgcccgaa ttcaaacacg catgcctcgg
     1381 cggcgtttat gggatgaagg gggcactgcg ccacctggcg ctcgatggcg caggcagcca
     1441 ggcgatcgtc gtttgcgcgg atattgccga atacgcgcgc ggcagcagcg gcgaaccgac
     1501 acagggcgcc ggcgcggtcg caatgctgct cgaggagaat ccgcggcttg ccgtagtgga
     1561 cctgatccgc tccggctcgg cctccgacta ccgcatcatg gacttccgca aaccaatgct
     1621 gcgtttctgc ggacaggacc gaagcgaaag ccaccaggtc caggacttcc ccgtcttcaa
     1681 cggcaagtat tccacgacct gctaccttga cgagacgctg cacgcgctca acgacatgta
     1741 cgagaagcgg ggattgcagg cgacggatta cctgcgcagc ctcaacaccg tgttcatgca
     1801 caggccgtat cgcaagatgc cggaaaccgg ctgggcggtg gcgtacctgt tcgcgctgag
     1861 caaaggtacc gccgaggatc gcgcggaact cgcttcctat tgctacgaag ccggtatcga
     1921 gcctgaggcg ctgcttcgcg aaatgctcgg caagccggaa atcgtcgcac tggcggagcc
     1981 ggagcggctg aatttcgaag tctatcccct gaccatggcg gtgtttcgca ttttccgtgc
     2041 ctcgcgccgt tatcgtcgcg agattctcga caagctcgca ctgggaagtg acaccatgct
     2101 ggacctcggc aacctctaca ccgcggcgct tcctgcctgg attgcagcag gcttcgagca
     2161 ggcgctggat gaaggccgtg agctggaggg cgaagaactg ctgacgctgg gttacgggag
     2221 cggcgacgca gcggaagtca ttccgttctt cgcggccggg cattggcggg aggcagctgc
     2281 gtcaattcgc ttcgcggccg ccatggaact ggctgtcgat ctcaaccgcg aacaatacga
     2341 agcactgcat gacggcaagc gggtcaacgg gcttgcctat atcccccaga acgaattcat
     2401 cattgataag gtcgggcaca gcgaccagcg ccagttccag gatatcggca tcgagtacta
     2461 tcgctacgta ggatagtcgg ccgggcgcga gcaggcgcgc ttgcaaccgg aactcaaacg
     2521 ccgctccgca agtacttgcc aaggacctgc gcgtggtctg ctttcagtcg ctgccattcc
     2581 agcttgaacc atggcgtgaa tcgcgcaggt ttctcctcga gcttccgctg cagttcggaa
     2641 gagctcagcc aaccggtatc ggcgatctcg ttccggttgg gcacaatgac gtcggccgtg
     2701 cgcccgaggt atacccagca gagttcattc tctgctccca gatcgtcata agatgcctgg
     2761 tacgtaaact tgtagacgaa ctcgagctcc gcatcgatgt tcaactcgtc acgcagccga
     2821 cggcgggttg cttcctgcat ggtttcgccg cgtctcgggt gactgcaaca actattggac
     2881 cagtagttcg gccacaggcg cttgtccccg cttcgctgct gcatcagcag ttttccatca
     2941 gggttgaaaa tgaacagtga aaacgccctg tgcaggagtc ctttggcatc gtgggctgac
     3001 tgcttgtcga tgtgccccat ctcgacgtcg ttctcgtcta caagtatgag ttgctccttc
     3061 tcggaggaga cgatcctgct cgcatgatct tgcatcgttt tccttaaacc gcgtggtccc
     3121 gttcaaaccc ggcgcggata acggcgtagc cggcatcgcg cagcgccagg gctacttgtt
     3181 gttctctgcc cggacagaga gcgacgatac atccaccgcc gcccgctccg gtcagcttcg
     3241 cgccggcggc gccgtgcttg cgggcaatgt ccaccatccg ttccagctct ggtgtggata
     3301 tgcctatcgc attcagaagg ccctggcaca ggttcatcag gatgcccagc gtccggtagt
     3361 cctggcggac gagcgcagca gctccttctg cgctgatcct gtccatctcg tcaaagatcg
     3421 cgttgtaacg cgcaggcgtg gctgcatgct ttgcagcaac agccgccacc tgctctttag
     3481 tgctgctctg cacgccgctc gacgcgatca cgatgggcgg aaactccgcg agttccagtt
     3541 tctgcatcgg ctgcgccgca cccttacagt acaacacggc cttttcatag gttgccaccg
     3601 tattgtcgat accggacggg ttgccatgtg caactttttc gcaggtgtat gcgagatcgt
     3661 tgactgcttc gttcgacagg ccaagtgcaa acacatgatc gaacgcgcgt atcacggcca
     3721 cggcgatggc agcggacgaa ccgagtccgc acgctctcgg cactgcagat tgcacgcgaa
     3781 cctcgaaatg cctgcccgct aacccgagct gccccacgac caggtttgcc agttcgtcaa
     3841 gcccgaccgc tggctgcgca agaggctggt cgtcgcttgc aaactccaac gtggtcctcc
     3901 ccgtgcactc ccgcacagaa gccgtcactc ccgaagcaag aggtagagcg agagccctcc
     3961 tgccgaagac cgcggcgtgt tcgcccaaca agatcacttt gcctgccgcg cggccgtgca
     4021 cctctggtgc acgattcgac tctgaagccc tcgactctcc acctgcagag atggctgcga
     4081 gcaagtcagc ggctttgcga accttgatct ctccgctttc gaccagccgc tcgacgatcg
     4141 cctcaaagtg ttccggcggg gtgccagcgc tcgccgcgac gtttctcgca tgcaggcgca
     4201 tatggccttc ctggatgccc ttcgtaacaa gcgcacgcag tgccgcgaaa ttctgtgcga
     4261 gaccaacggc agccatcagc cctgcaagtt cggcagaagt ggttgcgcct gtgagctgca
     4321 ggctcacagc gaccgcaggg ttggcctgca tcgacccgcc gacgacaccg accttcaacg
     4381 gcaactcgag gcgtcccgcg agattgccgc cgaggtcgac agtccagccc gtgagcgagc
     4441 ggtatccacc atttcgcgcc gcgaacgcat gcgcgccggc ctcgatcgcc ctccagtcat
     4501 tgccggtcgc aacggccacg gcgtcgatac cgttcaggat gcctttgttg tgcgtggcgg
     4561 cgcggtgcgc atcggcatcg gcaaaacgtc ccgccagcac gatggcatcg cgcactcctt
     4621 gtccgctgtg acccggttcg gccagtgccg aaaccgggaa cgtcgcgcgc gccgagacga
     4681 ccgaccggtc cgcgaggttc gaaagtatct tcatgacggc cgtgccggaa gtgagttcct
     4741 cgactctagg tgcgatacct tcgcacatcg tgttcactgt attcgcgccc atggcatcgc
     4801 aggtgtcgac gagcaaatgc aggacgatgt aagtatcgcc ggcggattct gctgccgcac
     4861 cgtctgccgc cagtcgcgca cggaagacct ccacatcgcg ggcgccaccg ccgcgcgcaa
     4921 caaggttcgg ctgcaggctg ttcgccagcg cgaccagctc ctgcgccgcc cgctcgaccg
     4981 ctgacatcgc ggcgtccgca tccgctactt tcgtgacgag aatctgcccg gcgaggaggg
     5041 attcgccgac gtcgacggag aatccgccgg aggctcgggc aagctttgca gctccgctga
     5101 ctgccgcaac gacagaggct tcctctacaa ccatcggcac gacgtagtcg cgtccgttta
     5161 cgagaaagtt cggtgccacc gccagcggca ggccgaacac cccgatgacg ttttcgatca
     5221 tgcggtcggc acgctcggca gacagcagtg atgcgccgct cttcagccgg gcggcggttg
     5281 cggcatccag ccatttccgt tccgtcaacg tatcgatacg ctcactgacg gacatgcgat
     5341 acaagccgcc aatgcgcgaa tctcttgcag gcggtctacc cattccttgc gtcaatgctc
     5401 atacatgagc ccggtgggct ccagcgttcg atccagcttg cgaaagccga gctggcctgc
     5461 gcggatgccg aactcggcaa ccgaagaatc cttcgcgtct cccggaagga aaacgatgcc
     5521 gatatctcct cccccggcgc cacagggctt gtaaaggaat ccgaggtcat ccgctgcctc
     5581 gcacagctcc tggtggcccg cgtcgaatac cccgaccccg tgctccagac tgaagcgcct
     5641 gagcgccgcc gtgtagaact ggagagattc gaatatgtcg ccgggctttt gcttgatcca
     5701 ggcatccagg acattccgcg acgcgtctac gagtttagcc gccgccgcgc gcgccgcgcg
     5761 attgcgctcg aatcgctcca gcttcctcag gcgctcggca gtactggcag gtttgccgct
     5821 ccacatcatc gagtaggaaa tgcccggcgg ccacttcagc gcctttatcc gctggtccgg
     5881 acgcacggcg aatgcaatca ggcccccgct cactgccgtg gcaacgtcca ccccactgcc
     5941 ttttccaccc tggaagtcga ggtgtgcgcg catcgccagt tggcgaatgt cgcgagcggg
     6001 acgtgtcgtc gcctcctccg gacctgcccg taccagcact gcagtaagcg cggccgcgag
     6061 tgccgaactg gaaccgaatc ccagcttgag gctgctctct gcatcgaaga acctgcgcgt
     6121 gtcgagcctg aacgacaggt tctcagccgg catggcattg accgttcgcc aaacgcactc
     6181 aaaaaggacc tggtcggcat tcggacggtc gccgttcccg tactcgtcga gccagtcgat
     6241 gctgccgcgc tcggtacagc ggaacctggc ttcggcggca aagccgggag acgccaccct
     6301 gtgacagtct tccctggtcg attcc
//
LOCUS       DASUOM010000343         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1578112,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000343 DASUOM010000000
VERSION     DASUOM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578112"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2034)
                     /locus_tag="VFY27_07260"
     CDS             complement(106..2034)
                     /locus_tag="VFY27_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX5765349.1"
                     /translation="MGNRLHAALASLFAAAGLPAAELAEMFGDSMVLQRDQPIMIWGT
                     ATPREAVSITLAGTVRSGEADGNGNWNITLPAMPAGGPHELVLRGAEASVRSLSDVLI
                     GDVWLCSGQSNMQFSVAASSGRGRDTFDTRESIRLFTVPQESDPAPQTRFGQTPQWRI
                     ADAGSIPEFSAACFFFAAELHETHPVPLGLIHASWGGSQIEAWMSPEALEKAGGYDEQ
                     LAMLATYASDASAGSAQFAALWEEWWTGVSATERPWEPATKDGDWHAVPAMQDWKAFG
                     DASLADHDGMVWYRKTFELDESQAKQDAQLHLGGIDEVDVTWINGKFVGSQFGWGTER
                     SYQVPHGLLRAGENQVVTNVLSTWDSGGLLGPKETVRVEFADGSERALAEGWQYRKVS
                     LEYGLPPRAPWESIGGLTGLFNAMIAPLQGLHLTGALWYQGETNAGRPEPYADLLRAM
                     IEDWRGRFGSLAFLVVQLPNFGSLPPAPSESGWAAIRDAQRRVALADPDTGLVVTIDA
                     GDNFDLHPPNKPVIGQRAAAVARAMLFGEEGVVDGIAPSHVRRSGEEVVLEFDPAVDA
                     LVVMSDSRPVAFELCADEPSGCDYAEARLEGNRVFLSSMKVVNATRVRHCWADAPICN
                     LYGKSGLPVASFEAKIRP"
     gene            complement(2054..4606)
                     /locus_tag="VFY27_07265"
     CDS             complement(2054..4606)
                     /locus_tag="VFY27_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5765350.1"
                     /translation="MLIERFGRVAAPGIILALLLGSCGDTAPPPAPDSGQKAQPADPW
                     PRIDPAPLDAEVEAFVADLLARMTLEEKVGQVIQAELRSVTPEDIKRYHLGSVLNGGG
                     STPNNDKHASLEDWVEFADAFYDASMDTSDGKLAIPILWGSDAVHGHSNVYGATIFPH
                     NIGLGAANNPGLLREIGAATAIEMLTTGLDWTFAPTLAVVRDDRWGRTYEGYSEDPRI
                     VAAYAKAIIEGLQGVPGTPEHLGDDKIMATAKHYLGDGGTDGGRDQGDNIDSEEDLVG
                     IHNAGYPPAIRAGVRSVMASFSSWQGRKVHGHEYLLTTALKERMGFNGFVVGDWNAHG
                     QVESCSNESCAPAFNAGMDMFMAPEDFRALYENLLAQVQEGAVSEARLDDAVTRILRV
                     KKEAGLFEAGRPSTRTHAAKQEELGSAAHRELARRAVRQSLVLLKNNGSVLPLAPASR
                     VLVAGDGADNIGKQTGGWTLTWQGTGNTNEDFPGATSVYAGIRETVEAAGGTAELNVE
                     GDFEERPDVAIVVFGEDPYAEFQGDAPHLQYESDNGPNLALLRSLKADGIPVVSVFLS
                     GRPMWINPHLNASDAFVAAWLPGSEGGGIADLLFEQPDGSMTHDFTGLLGYSWPRTAN
                     QADLNVGDENYDPLFAYGYGLTLAQGSELAVLPEESGLAGAVAGASGTYFADGRALTG
                     FSAMLGGDADNLLVVEGLEMPGNHLSPSMTMADREVQGDSSLLQWRDDAPGYFALRAD
                     GPPLDLQREANGQLSLSLLMNAPGSLSQPVMAGMRDAGGNAAQVDLTPLLEGSDGWQN
                     YRISLRCFAERGVNMQEVTVPLELYTTDEARLVLSEVRLATAAEGDAYCPAE"
BASE COUNT          800 a         1592 c         1476 g          876 t
ORIGIN      
        1 aataacgtgt aggagcgcgc catgcgcgcg agttcgccaa ggcggatcga actcgcccgc
       61 atggcgggct cttacaacgc ctggatcaac gtaacgcagg aactgctacg gtctgatctt
      121 agcttcgaag gatgcgaccg gaaggccgga tttgccgtag aggttgcaga ttggcgcgtc
      181 ggcccagcag tgccgcaccc gcgtcgcgtt gacgactttc atgctggaga ggaacacgcg
      241 gtttccttcg agccgcgctt ctgcgtaatc gcagccggac ggttcatccg cgcacagctc
      301 gaacgcgacc ggcctgctgt cgctcatcac gacgagcgca tcgacggcag gatcgaactc
      361 tagaacaact tcctcgccgc ttcgccgtac atgcgaaggc gcaatcccgt ccacaacccc
      421 ttcctcgccg aagagcatcg cgcgcgcaac ggcggcggca cgctggccga tgaccggctt
      481 gttcggcgga tgcaagtcga agttgtcgcc ggcatcgata gtcacgacga gtcccgtatc
      541 cggatcggcg agggcgaccc gccgctgtgc atcgcgaatc gcagcccagc cggattccga
      601 aggtgctggc ggcaacgagc cgaagttcgg cagttgcacc accaggaacg caagcgaacc
      661 gaacctgccg cgccagtctt cgatcattgc ccgcagcagg tcggcatagg gctccggcct
      721 tcccgcattc gtctcaccct ggtaccagag cgcgccggtg aggtgcaggc cctggagcgg
      781 cgcgatcatg gcattgaaaa ggccggtcag accgccgatc gattcccatg gtgcccgcgg
      841 cggtaacccg tattccagcg acaccttcct gtactgccat ccttccgcga gtgcacgttc
      901 gctgccgtct gcaaactcga ctcggacagt ttccttcggc cccaacagtc ccccggagtc
      961 ccaggtgctg agcacgttcg ttacaacctg attctcgccc gctcgtagca gtccgtgcgg
     1021 cacctggtaa ctgcgttcgg tgccccagcc gaactggctg cccacgaact tcccgttgat
     1081 ccaggtgacg tcgacctcgt cgattccgcc gagatgcagt tgcgcatcct gcttcgcctg
     1141 gctttcatcc agctcgaatg tcttgcggta ccacaccatg ccgtcgtgat cggcgagaga
     1201 tgcatcgccg aatgccttcc agtcctgcat tgccggtacg gcgtgccagt ccccatcttt
     1261 cgtggccggc tcccatggac gttccgtggc cgagacccct gtccaccact cctcccacag
     1321 cgcggcgaac tgcgcgcttc ctgcgctcgc gtccgacgcg taagtcgcga gcattgccag
     1381 ctgctcgtcg taacctccgg ctttctccag cgcttcagga ctcatccacg cctcgatctg
     1441 cgacccgccc caggacgcgt ggatcagacc cagcggcacc gggtgggtct cgtgcagttc
     1501 cgcggcaaag aaaaaacaag cggcagagaa ctcagggata gacccggcat cggcaattcg
     1561 ccactgcggc gtttgtccga agcgggtttg cggcgcagga tcgctctcct gaggcacggt
     1621 aaacagccga atgctttcgc gcgtatcgaa ggtgtcccgt cccctaccgc tcgacgcggc
     1681 caccgagaac tgcatgttcg actgccccga gcaaagccat acgtcgccga tcaatacgtc
     1741 ggacagcgaa cgaaccgatg cctctgcgcc acgcagtacg agttcgtgcg gaccgccggc
     1801 cggcatcgcg ggcagcgtga tgttccagtt cccgttgcca tctgcctcgc cggaacgaac
     1861 ggtgccggcg agcgttatgc tgacggcctc ccgcggagtt gcggtacccc agatcatgat
     1921 cggctggtcg cgttgcaaca ccatcgaatc gccgaacatc tcggccagtt cggccgccgg
     1981 caatcctgcg gcggcgaaca gcgatgccag cgctgcatgc aaccggtttc ccatcgttct
     2041 ggaacggacc ggcttactcc gccggacagt acgcatcgcc ttccgctgcg gtggccagtc
     2101 gcacctccga aaggacaagc ctggcctcgt ccgtcgtata gagctcgagg ggcaccgtga
     2161 cttcctgcat gttcacgccg cgctcggcga agcagcgcag ggagatgcgg tagttctgcc
     2221 agccatccga tccctcgagc agcggtgtca ggtccacctg ggcggcgttg ccgccggcgt
     2281 cgcgcattcc tgccatcacc ggctgtgaaa ggcttcctgg tgcgttcatc aacagggaca
     2341 gcgagagctg accgttcgct tcgcgttgta agtcgagcgg cgggccgtcg gcgcgcagcg
     2401 cgaaataccc cggagcatcg tcgcgccact gcagcagcga tgaatcgccc tgcacctccc
     2461 gatccgccat cgtcatggac ggtgacaggt ggttgcccgg catctcgagg ccttcgacaa
     2521 ccagcaggtt gtctgcgtcc ccgccgagca tggcggaaaa gccggtaagt gcgcggccgt
     2581 ccgcgaagta agttccgctg gctccggcaa ctgcgccggc gaggcccgac tcttcgggga
     2641 gcacggccaa ttccgaacct tgagcgagcg tgaggccgta gccgtacgcg aagagcgggt
     2701 cgtagttttc atcaccgaca ttcagatcgg cctggttcgc ggtccgcggc caggagtagc
     2761 caaggagccc ggtgaagtcg tgcgtcatgc tgccgtcggg ctgctcgaac agcaggtcgg
     2821 caatacctcc accttcgctt ccaggcagcc atgcggcaac gaacgcgtcg gacgcgttga
     2881 ggtgcggatt gatccacatg gggcggccgc tcaggaacac cgagacgacc gggatgccat
     2941 cggccttcaa ggagcgaagc aacgccaggt tgggtccgtt gtcgctctcg tactgcaggt
     3001 gcggtgcatc gccctggaac tcggcataag gatcctcgcc gaacacgacg atcgcgacat
     3061 cgggacgctc ttcgaagtcg ccttcgacgt tgagctccgc tgttccacca gccgcctcga
     3121 ctgtctcgcg aatgccggcg tagacggagg tagcgccggg gaagtcttcg ttcgtgttcc
     3181 cggttccctg ccaggtgagc gtccagccgc ccgtctgctt gccgatgttg tcggcgccgt
     3241 cgcctgcgac cagcacccgc gacgccggcg ccagcggcag cacggacccg ttgttcttga
     3301 gcagcaccag cgactgcctt accgcgcgcc gggcgagctc acggtgagcg gccgagccga
     3361 gctcttcctg cttagccgca tgcgtccttg tcgatggccg gcccgcttcg aacaagccgg
     3421 cctccttctt gactcgcagg atgcgggtga ccgcatcgtc gaggcgtgcc tcgctcacgg
     3481 cgccctcctg gacctgtgcc aggaggtttt cgtacagggc gcggaaatcc tcgggcgcca
     3541 tgaacatgtc catgccggcg ttgaacgcag gcgcacagct ttcgttgctg cagctctcga
     3601 cctgcccatg cgcgttccag tcgccgacca cgaagccgtt gaagcccatg cgctccttca
     3661 gcgccgtcgt gagcaggtat tcgtggccgt gcaccttgcg tccctgccag ctcgaaaacg
     3721 acgccatgac ggagcgaaca ccggcccgga tcgcaggggg gtagcccgcg ttgtggatgc
     3781 ccaccaggtc ttcctcggag tcgatgttgt ctccctggtc acgcccgccg tcggtgccgc
     3841 catcgccgag gtaatgtttg gcggtggcca tgatcttgtc gtcgccaagg tgttcgggcg
     3901 ttcccggcac tccttgcagt ccctcgatga tggctttcgc gtacgccgcg acgatgcgcg
     3961 ggtcttcgga gtatccctcg taggtgcgtc cccagcggtc gtcgcgcacc accgcgagcg
     4021 tcggtgcgaa cgtccagtcg aggccggtgg tgagcatctc gatcgccgtt gcggcgccga
     4081 tctcgcgcag cagtcccggg ttgttcgcgg caccaagccc gatgttgtgc gggaagatgg
     4141 tcgcgccgta gacgttgctg tgcccgtgca cggcgtcgga gccccacagg atcgggatcg
     4201 ccagcttgcc gtcggaggta tccatcgagg cgtcgtagaa cgcgtcggcg aactctaccc
     4261 agtcctcgag cgatgcgtgc ttgtcgttgt tcggcgtcga gccgccgccg ttcaggaccg
     4321 agcccaggtg atagcgcttg atgtcctccg gcgtgacgga gcgcaattcc gcctggatca
     4381 cctgccccac cttctcttcc agcgtcatgc gggcaagcag gtcggcgaca aacgcttcga
     4441 cttcggcatc cagcggggcg ggatcgatgc gcggccaggg atcggccggc tgcgccttct
     4501 ggccggagtc cggtgcgggc gggggggcgg tatcgccgca ggatcccagg agcaaagcaa
     4561 ggatgattcc cggggccgcc acgcggccga atctctctat cagcataacg tcatcagcat
     4621 aacgtcttcc ccaaagccgg tgagaattcg cggcctctga tcgacggcct gttcgctcga
     4681 ctgtacgacc agctgttagc ggtcacatgg ttctagtaaa cccgattgtt gcagtgcagg
     4741 tcca
//
LOCUS       DASUOM010000344         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_985140,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000344 DASUOM010000000
VERSION     DASUOM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985140"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..938)
                     /locus_tag="VFY27_07270"
     CDS             complement(123..938)
                     /locus_tag="VFY27_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5765351.1"
                     /translation="MQLNKATSQRGVTLVELLIGMAVGIVVVGGAIAMFTSSVRSSND
                     TLKSSKLNQEIGGLLHVIVNDVRRAGFWGNIDMDAIDENPFNLQGATALVVRDDMTSN
                     SIQPATGQGSCLLYSYDATYVPGNLAGALEATDLFGFRLNGSVVQMRQSGVVDGAGCV
                     GGTCNSCTNGTWENVTDPDLIEVTDLTFDLAASQCLNGSEPNAVDDNGDGVIDDAGEF
                     NCYVTVPAVGSGAVTVETRELLVTIAGRLSGDTSTQASVAQSVKVRNDLLRVR"
     gene            complement(929..1474)
                     /locus_tag="VFY27_07275"
     CDS             complement(929..1474)
                     /locus_tag="VFY27_07275"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5765352.1"
                     /translation="MDKQRGFTLLELLITVAVLAILATVAVPSMRSAAEKRRTIAAAE
                     EIYSQLQLARSEAIARSQPVFMNIEDGADWAIGISDDPACDPTDNAPACTLVDTNDAN
                     PITRRFTFNEHRNISILSTEDQINFLSQRGTATGANIDIVSTGDTGYVMSLVVGPLGQ
                     ITMCSSNADASKYISSYRPCN"
     gene            complement(1778..2101)
                     /gene="fdxA"
                     /locus_tag="VFY27_07280"
     CDS             complement(1778..2101)
                     /gene="fdxA"
                     /locus_tag="VFY27_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5765353.1"
                     /translation="MTFVVTEACIKCKLTDCVEVCPVDCFREGPNMLVIDPDECIDCT
                     LCEPECPVEAIFSEDEVPEDQRHFLKLNEELSRIWPVISEMKEPPEDADEWRDVKDKL
                     QHLER"
     gene            2262..>2772
                     /locus_tag="VFY27_07285"
     CDS             2262..>2772
                     /locus_tag="VFY27_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5765354.1"
                     /translation="MSPVSLARHGRISVVTIDNPPVNALSQHVRSGVMECFDKAEADP
                     ATSAIVLLCAGRTFVAGADIREFNLPPAEPHLPDVIARIETCTKPVLAAIHGTALGGG
                     LELAMACHYRLADSRAALGLPEVKLGLLPGATGTQRLPRLAGVETALGMMLSGDPVDA
                     DAALQAGLVD"
BASE COUNT          525 a          819 c          815 g          613 t
ORIGIN      
        1 gactcgtgta gacgctgacc atcgttatca gcaccagcag gaccaggcac ataaccaggg
       61 tagcggatcc cgcctgcctg tatttcactc ctgtcattgc tttaaaagta gtaaacactt
      121 tgtcacctga cgcgaagaag gtcatttctc accttcacgc tctgcgctac tgaagcctgt
      181 gtggatgtgt cgccggaaag acgtcccgcg atcgtgacca gcagttcccg tgtctcgacg
      241 gtaaccgcgc cgctgccaac agccgggact gtcacgtagc aattgaattc gccggcgtcg
      301 tcgatcacgc cgtccccatt gtcgtcgact gcattcggtt ccgagccgtt caggcactgg
      361 gatgcggcca ggtcgaaagt gagatctgtg acttcgatca ggtccggatc cgtcacgttt
      421 tcccaggtgc cgttggtgca gctattgcat gttccgccga cgcaaccggc gccatcgaca
      481 acgcctgact gccgcatctg gacgacggaa ccgtttagac gaaagccaaa aagatccgta
      541 gcttccaggg ctccggcaag attgcccgga acatacgtgg cgtcgtagct gtacagcagg
      601 cacgaccctt gccctgtggc tggctggatg ctgttgctcg tcatgtcgtc gcggaccacc
      661 agcgccgtcg cgccctgcag gttgaagggg ttttcgtcga tcgcgtccat atcgatgttt
      721 ccccagaatc ctgcgcgccg gacatcattc acgatgacgt gcaacaaacc gccgatctcc
      781 tggttgagct tgctgctttt cagcgtgtca ttgctgctgc gcaccgagga tgtaaacatc
      841 gcgattgcac cgccgactac cacgatgccg acggccatcc cgatcaggag ttctacgagg
      901 gtcactccgc gctggcttgt tgctttgttc agttgcatgg ccggtaactc gaaatgtact
      961 tgctcgcatc agcatttgaa gaacacatcg tgatctgccc cagcggcccg acgacgaggc
     1021 tcatcacata cccggtgtcc cctgtcgaaa caatgtcgat gtttgcgcca gtcgccgtgc
     1081 ctcgctgcga caggaaattg atctggtcct ctgtcgaaag aatgcttatg ttccggtgtt
     1141 cattgaacgt aaagcggcgg gttatcgggt tggcatcgtt ggtgtcgacc aacgtgcagg
     1201 ccggggcgtt gtccgtcgga tcacaggctg gatcgtcgct gattcctatc gcccagtcgg
     1261 ccccgtcttc gatattcatg aagaccggct gcgaccgtgc gatagcctcg gaccttgcca
     1321 actggagctg gctgtagatt tcctcggcag cggcgatggt cctgcgcttt tcagccgcgc
     1381 ttctcatcga aggaacggca acggtggcca ggattgcgag cacggcaacc gtgatcagca
     1441 attcgagtaa ggtaaatccg cgttgtttgt ccataaacct gtcatcttgc ttgaagcgcg
     1501 tcggcacact ttctccgatc gcttcttggg cttcatgccg tctggtgccg cggattattc
     1561 tctttcggga gaaggccctg ctatgtggca ggtcacaaca accggcttcg cgggccggtt
     1621 ttccgacgaa tgggagaatc ggagcgacga acggtacgct cagctcccgc gaagtgaggt
     1681 gtggggcgaa tattatggaa aatggcccaa gcgagagcgc cggcgactca aagcgggtgg
     1741 gcgtattccg ccgttacacg ggcagggttg aggagggtca gcgctcgagg tgctgcagct
     1801 tgtccttcac atcgcgccac tcatccgcat cctcgggagg ttccttcatc tcggagatta
     1861 ccggccagat gcgtgacagt tcttcgttga gcttgaggaa atggcgctgg tcctcgggga
     1921 cctcgtcttc ggaaaaaatc gcctcgaccg ggcactcggg ctcgcacagg gtacagtcga
     1981 tgcactcatc aggatcgatt accagcatgt tcggtccttc ccgaaagcag tcgacgggac
     2041 aaacttcgac gcagtcggtg agtttgcact tgatgcaggc ttctgtaacg acgaaagtca
     2101 tgaacgggtt ccttggactt gctgctcatt ttacgccatt cgccgcgtgc cgtatcaagt
     2161 gcgaatcagt gtgtggaacc tgggcgggac gatcctggct aattttgctt aatcgtcgcg
     2221 tttcgccaca ataatcccct ctgctgccga aaggatgaaa tatgagcccg gtcagtttgg
     2281 ctcgccacgg aagaatttcc gtggtcacga tagacaatcc gcccgtcaac gcactgtcgc
     2341 aacacgtgcg cagcggcgtc atggagtgct tcgacaaggc ggaggccgac ccggcgacgt
     2401 cggcgatcgt gcttctctgc gctggccgta ccttcgttgc cggcgcggac atccgggaat
     2461 tcaatcttcc tcctgcggaa cctcacctgc cggacgtcat cgcaaggatc gagacctgca
     2521 ccaaaccggt tctcgccgcg atacacggga cggcgctggg cggtgggctc gagcttgcga
     2581 tggcgtgcca ttaccggctc gcggacagcc gcgcggcgct cggtcttccg gaagtcaagc
     2641 tggggctcct gccgggtgcg acaggcacgc agcgtctccc gcggctcgca ggagtcgaaa
     2701 ccgctctcgg catgatgctt tccggggatc ccgtcgatgc ggacgctgcg ctgcaggcag
     2761 gcctcgttga ca
//
LOCUS       DASUOM010000345         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1080914,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000345 DASUOM010000000
VERSION     DASUOM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080914"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFY27_07290"
     CDS             complement(<1..853)
                     /locus_tag="VFY27_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765355.1"
                     /translation="MTRIAAHLNDAAITLLQNERIVYREPGFALLDERDLITGKDAFA
                     NARIHPRRVQHRYWTNLGTAPLEVQRFNHLSAADLVSRQLEQMWRTVDGSASELVVAV
                     PAYMDSSQLGLFLGIAGELKLPVIAMVDAAVAATRREYKNAVPVHVDIGLHTATLTRL
                     AQPGHAQVERVEVLQDCGIYALYDIWLNTLAETFVQQSRFDPLHTAATEQMLLDNLAT
                     WLARSAQRDRVELELTYGGISHKAEVESLALIGAAAPVYQPIASKLRALYRAEETPAI
                     QVTDRVAR"
     gene            complement(864..1664)
                     /locus_tag="VFY27_07295"
     CDS             complement(864..1664)
                     /locus_tag="VFY27_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5765356.1"
                     /translation="MKKKNIPVEFVFQLFALIISMIVVHAFYVSVVRPNAAQDIEQQA
                     IEREQDPGSVQERSMWVLIKDPEQEACFILMFWALAIMGYKAMVLLDERRLLETDLVP
                     IAEGMRILPEDTREFARQVQALPPERQRLLLPRVLLNALRRFSSTRNIQDVASSTHTI
                     CESEADRLESELSMIRYISWAIPSIGFIGTVRGIGDALAQADKAVQGDIAGVTQSLGV
                     AFNSTFIALLISIFLMFLVHQLQLMQERLVFDAENYTDDKLIRHMKSD"
     gene            complement(1749..>2016)
                     /locus_tag="VFY27_07300"
     CDS             complement(1749..>2016)
                     /locus_tag="VFY27_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765357.1"
                     /translation="GEAQEPDEQAETAPVTESGGADAGEGDAASDASDAESEAAGDPG
                     IDPAFDPDIYDPELDDQTYEGDDDVFVPTEEIPADEPIPFPSDI"
BASE COUNT          370 a          623 c          622 g          401 t
ORIGIN      
        1 agcgcgcaac acggtccgtc acctggatgg cgggcgtttc ttccgcgcga tagagcgcgc
       61 gcaatttgct cgcaatcggc tggtagaccg gggcggctgc accgatgagc gccagtgact
      121 cgacttcggc cttgtggctg atgccgccgt aggtcaattc cagttccacc cggtctcgct
      181 gtgctgagcg cgcgagccag gtggcgaggt tgtccaaaag catctgttcc gtggcggcgg
      241 tatgcagggg gtcgaatcgc gactgctgga cgaatgtctc cgcgagggta ttgagccaga
      301 tgtcgtacag ggcatagatg ccgcagtcct gcagcacttc gacccgctcg acctgggcat
      361 gaccgggctg cgcgaggcgc gtcagcgtcg ccgtatgcag gccgatgtcg acgtgaaccg
      421 gtaccgcgtt tttatattcg cgccgcgtcg ctgcgaccgc cgcgtccacc atggcaataa
      481 ccggcagctt caactcgcct gcaatgccca ggaacaggcc gagttggctg ctatccatgt
      541 acgcggggac tgcaaccacg agctcgctgg cgctcccgtc taccgttctc cacatctgct
      601 ccaactggcg gctgaccagg tccgcggcac tcagatggtt gaagcgctgg acctcgagag
      661 gcgcggtgcc gaggttcgtc caataccggt gctgcacgcg gcgcgggtgt atgcgcgcgt
      721 tggcaaacgc atccttgccg gtgatcaggt cgcgttcgtc gaggagcgcg aagccgggtt
      781 cccgataaac gatccgctca ttttgcagca gggtgatggc ggcatcgttc aaatgtgcgg
      841 caatgcgcgt cacggctttt cgactaatcg gacttcatgt gccggatgag cttgtcgtcg
      901 gtgtagtttt ccgcgtcgaa caccagccgt tcctgcatca gctggagctg atgcacaagg
      961 aacatcagga agatgctgat caggagtgca atgaaggtcg agttgaatgc aacgccgagg
     1021 ctctgggtga cgccggcgat gtcgccctgc acggccttgt cagcctgcgc gagtgcgtca
     1081 ccgataccgc gaaccgtccc gatgaacccg atggagggga tagcccagga gatgtaacgg
     1141 atcatcgaca gttccgattc gagccggtcc gcctcggatt cacagatcgt atgggtgctg
     1201 ctggcgacat cctggatatt ccgcgtagag gaaaaccggc gtagtgcatt caacagcacg
     1261 cgcggcaaaa gcaaccgctg tctttccggc ggcaacgcct gcacctgccg ggcgaattcg
     1321 cgcgtgtctt cgggcaggat ccgcataccc tcggcgattg gcaccaggtc cgtctccagc
     1381 aggcgccgct cgtccaggag caccatcgcc ttgtagccca tgatcgccag tgcccagaac
     1441 atcaggataa agcacgcttc ctgctccgga tccttgatca gcacccacat cgagcgttcc
     1501 tgtaccgagc cgggatcctg ctcccgctct atagcctgct gctcgatgtc ctgcgccgcg
     1561 ttcggccgca cgaccgatac gtaaaacgcg tgcacgacga tcatggagat gatcagcgcg
     1621 aaaagctgaa atacgaactc aaccggaatg ttcttttttt tcattcattt aaactcgcga
     1681 ctgtaagagt cgaggccgca cgatgccccg ccggcggact gaaaaccccg aagccgcaac
     1741 gaacgggatt atatatctga cgggaaaggg atcggctcgt ctgctggaat ttcctcggtc
     1801 gggacgaaga cgtcgtcgtc cccttcgtag gtctggtcgt ccagttccgg atcgtagata
     1861 tcggggtcga aggcagggtc gattcccggg tcgccggcgg cctcgctttc agcatcggac
     1921 gcgtcactgg cggcatctcc ctcgcctgca tctgcgccgc cggactcggt gaccggcgca
     1981 gtctccgcct gctcgtcggg ctcctgcgcc tccccg
//
LOCUS       DASUOM010000346         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1002047,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000346 DASUOM010000000
VERSION     DASUOM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002047"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFY27_07305"
     CDS             complement(<1..369)
                     /locus_tag="VFY27_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765358.1"
                     /translation="MKIIRAGRVLLIPLLLALTFAGCAATTLLYNHADWLIARQIDGY
                     FDLTRSQKTFVSSRLTGILTHHRHEALPRYESVLREATTRVQDGVSTDDLDWAFVQYD
                     QLRADLFGRFVSDGTEFVRQV"
     gene            complement(498..1268)
                     /locus_tag="VFY27_07310"
     CDS             complement(498..1268)
                     /locus_tag="VFY27_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5765359.1"
                     /translation="MRAFLGAVCAAHPAIAIPVTALLLGVTWFGINWAYHAAKKPSEI
                     LFPLDNAFDKNLTETWREYGPLFREHATAAITPELLAALAQVEGSGNPLARTYWRWHS
                     SWNPLQWYRPASSAVGMFQITSGTFQAAKRYCIHDHVVTEDGPWHDIRSCWFNGLYTR
                     VLPSHAIELTAALLDRQVAQAIGSRSGVTSQQKQELAAIIHLCGASVGQAYVSRGFRL
                     PPRQNCGEHEVRTYLRKVSAAKRQFARLATAAQTTSWK"
     gene            complement(1632..>2189)
                     /locus_tag="VFY27_07315"
     CDS             complement(1632..>2189)
                     /locus_tag="VFY27_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5765360.1"
                     /translation="EEASLGISQASVVEDEQKLRERVGFIHESVGTDALVEQFIEGRE
                     LYVGVMGNQRLRVFPVWEMQFSKMPDNVHRIATERVKWSNKYQEKYGIQTAELKDASD
                     SVVERIQHLAKRVYRSLDLSGYARIDLRMDRDEKLYVLEANPNPQIAEGEDFAESAKQ
                     AGMPYKQLLQQIITLGLQWRPERNG"
BASE COUNT          452 a          616 c          651 g          470 t
ORIGIN      
        1 gacttgccgg acaaattctg ttccatccga gacaaagcga ccgaagagat ccgcccgtag
       61 ctgatcgtat tggacgaagg cccaatccag gtcgtccgtc gacaccccgt cctgtacacg
      121 tgtcgtggcc tcgcgcagca cggattcata gcggggcagg gcttcatgtc gatggtgcgt
      181 taagatgccg gtcagcctcg atgacacgaa cgtcttctgt gagcgggtga gatcgaagta
      241 cccgtcgatc tgacgtgcga tcaaccaatc ggcgtggtta tacagcagtg tcgtggcggc
      301 acagccggcg aacgtcagtg ccaacaagag gggaatgagc aggactcgtc ctgcacgtat
      361 gatcttcatc ggttgtgatt gcctaacgat ctagatgcgg tgagtatact gtcacggtac
      421 cacagattct gtcccgctgc tagtcagtca gcctccatgg ctttatcctt actatgtagt
      481 gagttacaga aacatcatca cttccagctt gtggtttgtg cagctgtggc gaggcgagcg
      541 aattgccgtt tggcagcgga caccttccgg aggtaggttc gtacctcgtg ctctccgcaa
      601 ttctgccgcg gtgggagccg aaatccacgc gagacgtacg cctgtcccac cgaggctccg
      661 cacaagtgaa tgatggcggc aagctcttgc ttctgctgtg aggtgacgcc tgatcgtgac
      721 ccgatcgcct gcgcgacctg acgatccagc agggccgccg tcaactcgat cgcatggctg
      781 ggcaacacac gggtatacag cccattgaac cagcaggacc gtatgtcgtg ccagggcccg
      841 tcttctgtca cgacatgatc gtgaatgcag taacgcttag cggcttgaaa cgtaccggag
      901 gtaatttgga acatcccgac ggcactcgaa gctggtcggt accattgcag ggggttccac
      961 gaggagtgcc agcgccagta ggtgcgagca agcggattgc cgctgccttc gacctgggcc
     1021 agcgctgcaa gtaattcagg ggtgatggcg gccgtcgcat gttcgcgaaa cagagggccg
     1081 tattcacgcc atgtttcagt gagatttttg tcgaaggcgt tgtccaacgg aaacaatatt
     1141 tcgctcggct tcttggccgc atgatatgcc cagttgatgc caaaccaggt cacaccaagc
     1201 agcagggcag tgacaggaat cgcgatcgcc ggatgagcgg cgcacacggc gcccaggaag
     1261 gctcgcattt cgtgcgagcg ccagcggatg aagcgccaca gcttcttacc gctacggcgg
     1321 gggcgtgact gccggcgcga ggcacgacgg ttggagcggg gaggcatgga aacactatag
     1381 ctgaatcaca gaaaaatgga tcaagcaatg agaaatcgat catgagagga tgaaaagcgg
     1441 gacgtcctgc ctgcttgaat gctcgatcgg acaactcgtt gcaacaacgc agtttcgaca
     1501 tcagttgcgt ctacggcggt ggtcgatcgg aacggcggag tctcactggt gcattgcaga
     1561 cgctgtgggt cggcggcgcg gtacaagatg cgttcatcgg tcggggcttg tcaggaactg
     1621 gcgcatcccc gtcagccatt gcgttccggc cgccattgaa ggcccaaggt gatgatctgc
     1681 tggagcagct gcttgtaggg catgcctgcc tgtttggcgg actcggcgaa atcctcgccc
     1741 tccgcaattt gaggattggg attcgcttcc agcacataga gcttctcgtc cctatccatc
     1801 cgtaggtcga tccgcgcata cccgctcagg tccaaggaac ggtagacgcg cttcgccaga
     1861 tgttggatgc gctcgacgac gctgtcggac gcgtctttca gttccgcggt ctgaataccg
     1921 tatttctcct ggtacttgtt gctccacttc acccgctccg tggcgatccg atggacgttg
     1981 tctggcatct tgctaaattg catctcccac acgggaaaga ctcgcagtcg ttggttgccc
     2041 atcacgccga cgtagagctc gcggccctcg atgaattgtt cgacaagtgc atccgtgccg
     2101 acgctctcat gaatgaatcc aacccgttcg cgcaacttct gttcatcctc tacgacggag
     2161 gcctgtgaaa tcccgagcga ggcttcctc
//
LOCUS       DASUOM010000347         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_753773,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000347 DASUOM010000000
VERSION     DASUOM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753773"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFY27_07320"
     CDS             <1..428
                     /locus_tag="VFY27_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765361.1"
                     /translation="DVAAEATRRISIFTHDLEPGIYDDPDFLEVVKRLVLSQAYARVR
                     VLIADPARAVKNGNNFVHLGRRLNTYIEFRHVREDLRTHAESFCIADEVALVYRLQAS
                     RWEGIADTYEPGVAKVYSKLFDEIWVASEVETEFRQLGI"
     gene            443..934
                     /locus_tag="VFY27_07325"
     CDS             443..934
                     /locus_tag="VFY27_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5765362.1"
                     /translation="MLPTDLTVSAVVQRDGRYLIVEERAAGVIVFTQPGGHIEAGESP
                     EQAVVRETLEETRCTVEVETLLNVYLWIQPQTRQQFLRIVYVARFAGENPALALDDGI
                     HAVHWFTLDDLNRRRRTLRSPVVTRCIEDYVAGRRKPESLLDGLLPVQQNVERVIASA
                     SLV"
     gene            972..2153
                     /gene="mnmA"
                     /locus_tag="VFY27_07330"
     CDS             972..2153
                     /gene="mnmA"
                     /locus_tag="VFY27_07330"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5765363.1"
                     /translation="MVHPDSPSFQPPPEVLRGRRIIVGLSGGVDSAVAALLLKDQGAD
                     VHALHMTNWEDDDAYCTAAADLQDARRVCEQLDIPLHHVNFARQYRERVFEYFLHEYR
                     AGRTPNPDVLCNREIKFGVFRTHAGRLGAEWLATGHYARIRRGSSRGETTVQLWKAAD
                     RNKDQSYFLHAVTEEALAETLFPLGELTKEQVRCLAQDRGLNVFDKKDSTGICFIGER
                     PFREFLAQYLPARPGPIRTPEGKTIGEHGGLMYYTPGQRQGLRIGGRKDSSELPWYVA
                     AKDLASNALIVVQGEHPLLYSDELQATDVAWIGETPAALSSAKRLSCRAKVRYRQQDQ
                     ACRVRSSGDRGLLVSFEKPQRAVAPGQFVVFYEGDRCLGGAVIDRVLSPAADVAREAA
                     I"
     gene            2353..>4230
                     /gene="acnA"
                     /locus_tag="VFY27_07335"
     CDS             2353..>4230
                     /gene="acnA"
                     /locus_tag="VFY27_07335"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5765364.1"
                     /translation="MKDSFGARSMLDVDGTQYEIYRLDAVKEGHAGRLPYSLKILLEN
                     LLRHEDGRDVTREDILALANWDPKAEPDTEISFTPARVILQDFTGVPAVVDLAAMRDA
                     VVKLGGTADSINPLSPAELVIDHSVQVDRYGAADSLDLNNKIEFKRNKERYTFLRWGQ
                     EAFENFRVVPPNTGIVHQVNLEYLGRVVFDVSKNGTRSAWPDTLVGTDSHTTMINGLG
                     ILGWGVGGIEAEAAMLGQPITMLIPQVLGFKLSGALREGTTATDLVLTVTEVLRRKGA
                     VGKFVEFFGDGLRELPLADRATLGNMSPEFGSTCAIFPIDGETIRYLELTGRDPAQIR
                     LIETYAKAQGLWRYEGAQPAEYSDLLELDLGSVEPSIAGPKRPQDRILLTEADDRFRE
                     HLAVQTENRPSGGAATLELDGEAAEIGDGAVLIAAITSCTNTSNPAVMIGAGLLARNA
                     HARGLRTKPWVKTSLAPGSKVVTDYLEKADLLDDLAALGFYIVGYGCTTCIGNSGPLK
                     PEIAEAVVRHNIVGCAVLSGNRNFEGRIHPMVQMNFLASPPLVVAYALAGTMHLNLLK
                     DPLGNDANGQAVYLRDIWPTQKEIQDFISRNVNSKMFKSSYASVFAGDRNWNSLDVPS
                     GE"
BASE COUNT          827 a         1330 c         1333 g          740 t
ORIGIN      
        1 ttgacgttgc tgccgaggct acgcgccgca tctccatatt tacgcacgac ctggaaccgg
       61 ggatctatga cgacccggat ttcctcgaag tcgtaaaacg ccttgttcta tcccaggcat
      121 atgcgagggt ccgcgtattg atagccgatc cggcgcgcgc ggtgaagaac ggcaacaact
      181 tcgtacatct cgggcgtcgc ctgaacacct acatcgagtt ccggcacgtg cgggaggatc
      241 tgcgaacaca cgcggaatcc ttttgcattg ctgatgaagt ggcgctcgta taccgcctcc
      301 aggcgagccg ctgggaaggt atcgccgaca cttacgagcc gggtgtcgcg aaggtgtaca
      361 gcaagctgtt cgatgaaatc tgggtagcaa gcgaagtgga aaccgaattc cgccagctcg
      421 gcatctgaag tcccttgccc gagtgttacc caccgacctg acagtatccg ccgttgttca
      481 aagagacggc cgctacctga ttgttgaaga acgggcagcg ggagtgatcg tctttacgca
      541 gccgggcggg catatcgaag ccggggagtc gccggaacag gcggttgttc gcgaaacgct
      601 ggaggaaacc cgctgtaccg tagaggttga aaccctgctc aacgtctacc tctggattca
      661 gccccagacc cgccagcagt tcctgcgcat cgtctacgtg gcgcgatttg ccggggaaaa
      721 ccccgccctc gccctggacg acggcatcca tgcggtgcac tggttcacgc tggatgacct
      781 gaatcgtcgc cgcaggacac tgcgttcccc cgtggtaacg cgctgcatcg aagactacgt
      841 ggcggggcgg cgaaagccgg aatcgctgct ggatggcctt ctgcccgtgc agcagaacgt
      901 ggagcgcgtg atcgccagcg catcgctcgt ttagtttctt cgattgcccc ggatgataaa
      961 atccggggta catggttcat ccggattccc cgtcgttcca gccaccgccc gaagttctcc
     1021 gtggccgtcg catcatcgtc ggcctgtcgg gcggcgtcga ttcagccgtg gcagcgcttc
     1081 tgctcaagga ccaaggggcg gacgtgcacg ccctgcacat gacgaactgg gaagacgacg
     1141 acgcatactg cacggcagcc gccgacctgc aggacgcccg gcgcgtctgc gagcaactcg
     1201 acatccccct gcaccacgtg aacttcgcac gccagtatcg tgagcgtgta ttcgagtatt
     1261 tcctccacga gtaccgcgcc ggccgcacgc cgaacccgga tgtattgtgc aaccgcgaaa
     1321 tcaagttcgg cgtgtttcgt acccacgccg ggcggctggg cgcagagtgg ctggccaccg
     1381 gccactatgc ccggattcgc cgaggcagta gccgtggcga aaccacggtg caactgtgga
     1441 aggccgcaga caggaacaag gaccaaagct acttcctgca cgcggtaacg gaagaagcgc
     1501 tggccgaaac cctgttcccc ctcggtgagc tgacgaaaga gcaggtccgg tgcctcgctc
     1561 aagaccgggg actcaatgtt ttcgacaaga aggacagcac cggcatctgc ttcataggag
     1621 agcggccgtt tcgcgagttc ctcgcgcagt atctcccggc ccgtcccggg cccatccgca
     1681 cgccggaagg caagacgatc ggcgagcacg gcggactcat gtactacacg ccggggcagc
     1741 gccagggcct tcgcatcggt ggccgcaagg acagttccga gctgccctgg tacgtagcgg
     1801 ccaaggattt ggcgagcaac gccctgatcg tagtgcaagg cgaacacccc ctgctctaca
     1861 gcgatgagtt gcaggcgacc gacgtggcct ggatcgggga aacacccgct gcactctcga
     1921 gcgcgaagcg cctgtcctgc cgggcgaaag ttcgctaccg gcagcaggac caggcctgcc
     1981 gtgtccgaag ctccggcgac agagggttgc tggtgtcttt cgaaaagccg caacgggcgg
     2041 tcgcccccgg acagttcgtc gtgttctacg agggtgaccg ctgcctgggc ggcgccgtta
     2101 tcgaccgcgt gctttcgccc gccgcggacg tggcccgcga ggccgcgatt tagcgcgcgg
     2161 tattcgttat aatgccggcc cgcgccgcct tggcagcgac cgttcgccgg ccgctcattg
     2221 catcattcgt gaacggcact gcgtgtcagc ggaatccagg tccgaggctt cgccagtgcc
     2281 gggcctcgag cgttcacgaa tgatgcgaac cacgtcggcc cggacctagc caaccgtccc
     2341 cggagagaaa ctatgaagga cagcttcggc gctcgttcaa tgcttgacgt tgacggcacg
     2401 cagtacgaga tctatcgact cgacgccgtc aaggagggtc atgcaggccg gctgccctac
     2461 tcactcaaga tcctgctcga gaacctcctg cgccacgaag acggccgcga cgtaacgcgc
     2521 gaagacatcc ttgcgctggc caactgggat ccgaaagcgg agccggatac cgaaatttcc
     2581 ttcactcccg cgcgtgtgat cctgcaggac tttaccgggg taccggcagt cgtggacctg
     2641 gcagcaatgc gcgatgccgt cgtgaaactg ggcggcacgg cagacagcat caaccccctg
     2701 tcaccggcgg aactggtcat tgatcactcc gtgcaggtgg accgctacgg tgcggcggat
     2761 tcgctggacc tgaacaacaa gatcgagttc aagcgcaaca aggagcgata taccttcctg
     2821 cgctgggggc aggaagcctt cgagaacttc cgcgtcgtac cgccgaatac gggcatcgtg
     2881 caccaggtca acctggagta cctcggcagg gtcgtgttcg acgtatcgaa gaacgggacg
     2941 cgttccgcct ggcccgatac gctggtgggc actgactctc acaccacgat gatcaacggg
     3001 ctcgggatac tcggctgggg cgtgggcggc atcgaggccg aagccgcaat gctgggacag
     3061 ccgattacca tgctgatacc gcaggtactc gggttcaagc ttagcggtgc cctgcgcgag
     3121 ggcaccaccg ctaccgacct ggtgctgacc gtgaccgagg tgctgcgccg gaaaggcgcc
     3181 gtcggcaagt tcgtcgaatt cttcggggac ggtcttcggg agctgcccct tgcggaccgg
     3241 gccaccctcg gcaacatgtc gccggagttc ggcagcacct gcgcgatctt tccgattgac
     3301 ggtgagacca tccgctacct ggagctcacg gggcgcgacc cggcacagat ccgcctcatc
     3361 gagacttatg cgaaggccca gggtctctgg cgctacgaag gcgcacagcc ggcggagtac
     3421 agcgacctgc tggaactgga ccttggcagc gtggagccga gcatcgcggg acccaagcgt
     3481 ccccaggatc gcattctcct gaccgaggct gacgaccgct ttcgcgagca cctcgcggtc
     3541 cagacggaga accggccgtc cggcggcgcg gcaacgctcg agctcgacgg cgaggcagcc
     3601 gaaatcggcg atggcgccgt gctgattgcg gccattacca gttgcaccaa tacgtcgaat
     3661 cctgccgtca tgatcggtgc gggattgctg gcgcgaaacg cccacgcccg cggcctgcga
     3721 acgaagccct gggtgaaaac cagcctggcg ccgggatcga aggtggtgac ggactacctg
     3781 gagaaggcgg atcttctcga cgatctcgcg gcgctgggct tctacatcgt aggctatggc
     3841 tgcacgacct gcatcggcaa ctccgggccg ctgaagcccg agatcgcgga ggcggttgtg
     3901 cgccacaaca tcgttggttg tgcggtgctg tccggcaacc ggaacttcga aggccgaatt
     3961 cacccgatgg tacagatgaa tttcctggcc tcgccgccgc tggtcgtcgc ctatgcactc
     4021 gccggaacga tgcacctgaa cctgctgaag gacccgcttg gcaacgacgc caacgggcag
     4081 gctgtctacc tccgggacat ctggccgaca caaaaggaga tccaggactt catttcgcgg
     4141 aacgtcaact cgaagatgtt caaatccagt tatgcgagcg tgttcgcggg cgacaggaac
     4201 tggaacagcc tggatgtgcc gtcgggagag
//
LOCUS       DASUOM010000348         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1697765,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000348 DASUOM010000000
VERSION     DASUOM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697765"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1090
                     /gene="nadC"
                     /locus_tag="VFY27_07340"
     CDS             239..1090
                     /gene="nadC"
                     /locus_tag="VFY27_07340"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5765365.1"
                     /translation="MNDLQSLIEKNVSDGLAEDLGVGDLTAALVHPGTTAEATIVARE
                     SMVQAGRPWVDEVYRQLDTRIAIDWTAADGDTVAAGATVAFLRGPARPLLSGERTALN
                     FLQMLSSTATTTARFVEAVSGTRAKILDTRKTIPGLRLAQKYAVRCGGAVNHRIGLFD
                     AILIKENHILGAGGIQAAVAEARRLHPGVPVEIEVESIDELRQALHARAERLLLDNFS
                     VDQLREAVAVNRKEGDPPALLEASGNLSLSTVRTVAETGVNFISIGALTKNIDAIDLS
                     MRFKSPL"
     gene            complement(1174..>2361)
                     /locus_tag="VFY27_07345"
     CDS             complement(1174..>2361)
                     /locus_tag="VFY27_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765366.1"
                     /translation="ETSYALCYLDLDQFKIVNDSCGHSAGDALLGQLGALLKSKIRWR
                     DTLARLGGDEFGVLLESCSLDEAMQTAEILRAAIGDFKFLWDDRSFRLGVSIGVVPIT
                     ADNEDVASLMSAADSACAAAKEAGRNRIHSFLENDIDLMRRRREMQWAARINNALDED
                     RFELFRQTIQPLQTAVDGAHYELLLRMRDETGGIVAPGLFIEAAERYGITPSIDRWVI
                     KNAFRWLVSEADERERLSLCSINLSGQSLGDEKFLPFVIDQFQMSGLDATKICFEITE
                     TAAIASYSQANRFINALKELGCKFALDDFGTGLSSFGYLKHFPVDFLKIDGSFVKEIL
                     HDPIDREMVRSINEIGHLTGKQTIAEFAENEEIITMLRGMGIDYAQGYGVAEPKRVTR
                     AVA"
BASE COUNT          440 a          732 c          726 g          463 t
ORIGIN      
        1 cgggagctga cgaaatcgcc aaggcgaagc gcactgcgtt cctcgcctca cggaccaaca
       61 cgctcctgtc gattcccatg atcatgtgca tggtcggagc cgggcatggt ttcttcatgt
      121 agatgagtcg cagcaccgga agcccggcac gtcggccgtg ccgggctttt ttgtgcgcgc
      181 aatcccgctg gtttctctat gatagatgcg gtctggaccc ctcccttcct gaaccgcgat
      241 gaatgacctg cagtccctga tcgaaaaaaa cgtcagcgac gggctcgccg aggatctggg
      301 tgtcggcgat ctcacggcgg cccttgtcca cccgggaaca actgccgagg cgacgatagt
      361 agcgcgtgag tccatggttc aggccggccg cccatgggtg gacgaagtgt accgtcagct
      421 cgacacccgg atcgccatcg actggacggc cgcggacggc gatacagtcg cggccggtgc
      481 gaccgtggct ttccttcgtg gccctgcccg tccgctgctg agcggcgaac gcacggcgct
      541 caattttctg cagatgctat cgtcgactgc gacaaccaca gcccgcttcg tcgaagccgt
      601 gtccggcaca cgagcgaaaa tcctcgatac ccgcaagacc ataccgggat tgcggctcgc
      661 gcagaagtac gccgtgcgtt gcggcggcgc tgtgaaccac cggatcgggc tgttcgacgc
      721 gatattgatc aaggagaacc acatccttgg cgccggcggc atccaggcgg cagtcgccga
      781 ggcgcgccgg ctgcacccgg gagttccggt cgagatcgag gtcgagtcga tcgatgagct
      841 gcggcaggct ttgcacgcgc gggcggagcg cttgctgctg gacaacttca gcgtcgacca
      901 gctgcgggag gcggtggcgg tgaaccgcaa agaaggcgat ccgccggctt tgctggaagc
      961 ctccggcaat ctgtcccttt cgacggtaag gaccgtagcg gagaccgggg tgaatttcat
     1021 ttcgatcggc gcgctgacga agaacatcga cgcaatcgat ctgtcgatgc ggttcaaatc
     1081 accgctgtag gagctcgcca tgcgagcaag tgcgacgcaa cttgtgcaga acctgtgaaa
     1141 tcgcccgcac ggcgggctcc tgcagagccg tcgctatgct accgcgcgtg tgactcgctt
     1201 cggttccgcc actccgtaac cctgggcgta gtcgatgccc ataccgcgaa gcatcgtgat
     1261 gatctcttcg ttctcggcga actcggcgat cgtctgcttg ccggtcaggt gtccgatctc
     1321 gttgatcgat cgcaccatct cgcggtcaat cgggtcatgg agtatttcct tcacgaagct
     1381 gccgtcgatc ttgagaaaat cgacggggaa gtgcttcagg taaccgaacg aagaaagccc
     1441 ggtcccgaaa tcgtccagcg cgaacttgca gcccagctcc ttcaacgcgt tgatgaaacg
     1501 gttcgcctgc gagtaactgg ctattgcagc cgtttccgtt atttcaaagc aaatcttggt
     1561 ggcatcgagc ccgctcatct ggaactggtc aatgacgaat ggaaggaact tttcgtcgcc
     1621 aaggctctgc ccggacaggt tgatggaaca cagactcagt cgctcccgct cgtccgcttc
     1681 cgaaaccagc cagcggaacg cgttcttgat cacccagcga tcgatgctcg gtgtgatccc
     1741 gtaacgctcc gcggcctcga tgaacaggcc gggtgcgacg atgccgccgg tttcatcccg
     1801 catccgcagc agcagctcgt aatgcgcgcc gtctacggca gtctgcaacg gctggatagt
     1861 ctgcctgaac agctcgaagc ggtcttcgtc cagcgcattg ttgatccgcg cggcccattg
     1921 catttcgcgg cgccggcgca tgaggtcaat gtcgttctcg aggaagctgt ggatgcggtt
     1981 gcgtcccgcc tccttggcag ccgcacatgc gctgtcggcg gcactcatga gcgaggcgac
     2041 gtcctcgttg tcggcggtga tcgggactac gccgatgctc acgccgagac ggaagctgcg
     2101 gtcgtcccaa agaaacttga agtcgcctat tgctgcgcgg aggatctcgg ccgtctgcat
     2161 cgcctcgtcg agactgcagc tttcgagcag gacgccgaac tcgtctcccc cgaggcgcgc
     2221 cagcgtgtcg cgccagcgaa tcttcgattt gagtagtgcg ccgagctggc ccagcagcgc
     2281 atcgccggcg ctgtgcccgc aggaatcgtt gacgatcttg aactggtcga gatccaggta
     2341 acaaagcgca tacgacgtct c
//
LOCUS       DASUOM010000349         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1093630,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000349 DASUOM010000000
VERSION     DASUOM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093630"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VFY27_07350"
     CDS             complement(<1..1697)
                     /locus_tag="VFY27_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5765367.1"
                     /translation="MGAEIARIEGREFDAMRLYKRAIRSAGANGFVHNEALAYEIAAR
                     FYGARGFDKIANMYLREARYCYLRWGADGKVSQLDMLYPHLAEAEPARGTASTIVAPG
                     EILDLATVITVSQTVSGEMILEKLVDRVMRAAIQHAGAERGLLITPRGDDLRLEAEGT
                     TSPDGVTVRMHDSAGAAAFPESLVRYVMRARENVILDDASSGNSFSAHPYIGQHRARS
                     ILCLPLVNRRKFTGVLYLENNLTPRVFTPDRIVVLKVLASQAASALENARLYRDIEHR
                     EAEIRALKDQLYRENLALRDEVDRTSMFEEIVGTSKALKAVLSRVAKVAPTDSTVFIT
                     GETGTGKELIARAVHKRSRRAGRAFVSVNCAALAPALIPSELFGHEKGAFTGATQRRL
                     GRFELADGGTIFLDEVGDLLSETQVALLRVLQEREFERVGGTQPIRVDVRLIAATNRD
                     LQAAVDSGTFRQDLFYRLNVFPIEAPPLRERKDDVLMLVEYFLHRYAARTGKRFRSID
                     KRTLDLLRAYDWPGNIRELQNVIERSVILSAGDVFSVDELWLSGASPRAATRVQSSVS
                     "
     gene            1896..>2283
                     /locus_tag="VFY27_07355"
     CDS             1896..>2283
                     /locus_tag="VFY27_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765368.1"
                     /translation="MGIQRRVDGGGMIPGKEASLRLSGPIPELRQTQHRVARQVALKS
                     TFIKPFIVEAAECRSQPTQRSDNLDLRSDEVDHEAEARSPGKLETILGFRLYIAKPIS
                     HCQEIRDQLVPAICRERKITNPVRGVE"
BASE COUNT          473 a          709 c          683 g          418 t
ORIGIN      
        1 gacacggacg attgaacgcg agttgccgca cgaggcgacg ctccagacag ccacagttcg
       61 tcgaccgaaa agacgtcgcc ggcactcaag atcaccgagc gttcgatgac gttctgcaac
      121 tcgcgaatgt tccccggcca atcgtaggcc cgcaacaggt ccagcgtccg cttgtcaatc
      181 gagcggaacc gcttgccggt tcgcgcggca tagcgatgca ggaaatactc gaccagcatc
      241 aggacgtcgt ccttccgttc tcggaggggc ggtgcctcga tcggaaacac gttcagacga
      301 taaaacaggt cttgacggaa agtcccgctg tcaaccgcgg cctgcaggtc acgattcgtg
      361 gcggcgatga gccgaacgtc gacacggatg ggttgcgtgc ctccaacgcg ttcgaattcc
      421 cgttcctgga gcacccgcag gagcgcgacc tgggtctccg acaggaggtc acccacttcg
      481 tcgaggaaga tcgtcccccc gtcggcaagc tcgaaacggc caagccggcg ctgcgtggca
      541 ccggtgaacg cgcctttctc atggccgaat aactcggacg gaatcagcgc tggcgccagc
      601 gctgcacagt tcacgctgac gaaggcgcgt ccagccctgc gggatcgttt gtgaaccgcg
      661 cgagcgatga gttccttgcc cgtgccagtc tctccggtga tgaacaccgt ggagtcggtc
      721 ggagcgacct tggcaacccg cgacaggacc gctttcagcg ccttcgaagt cccgacaatc
      781 tcctcgaaca tcgaggtccg gtcgacctcg tcgcgcagcg ccagattttc gcggtagagc
      841 tgatccttga gggcgcggat ctccgcctcg cgatgttcaa tatcgcggta gagccgcgca
      901 ttttccaggg cgctcgccgc ttgcgatgca agcaccttca gcaccacgat ccgatcggga
      961 gtgaacacgc gcggcgtcag attgttttca aggtagagga caccagtgaa cttacgtcgg
     1021 ttgacaagtg gcagacagag aatggaacgg gcacgatgct gaccgatgta gggatgtgcg
     1081 gaaaacgagt tcccggacga ggcatcgtcg aggatgacgt tctcccgagc gcgcatgacg
     1141 taccggacga gcgactccgg aaacgcagcg gcccctgcgc tgtcgtgcat acgaacggtc
     1201 acaccgtccg gactggtcgt cccctcggcc tcgagccgca gatcgtcacc tcgcggagtg
     1261 atcagaagac ctcgctcggc gcccgcatgc tggatcgccg ctcgcatgac cctgtcgacc
     1321 aacttttcca gaatcatctc gcccgacact gtctgggaca ccgtgatgac agtcgcgaga
     1381 tccaggatct cgcccggggc cacgatcgtg ctcgctgtgc cgcgagctgg ttccgcttcc
     1441 gcgaggtggg gatagagcat gtcgagttga ctgactttgc catcggcccc ccaacggaga
     1501 tagcaatagc gggcttcccg caaatacata ttcgcgattt tgtcgaaacc gcgtgccccg
     1561 tagaaccgcg cagcgatctc gtaggccagc gcctcgttgt ggacaaaccc gtttgcgccc
     1621 gctgagcgga tcgcacgttt gtagaggcgc atggcatcga actcgcggcc ctctattcgt
     1681 gcgatttcgg cgcccaccaa tgcggcacgg ttttcaaaat tctccggaca atgctgcgcc
     1741 cagatttcga gctgtctgaa gtgagcggct aaagcgcaga aatgctgctg cttctgttcg
     1801 ggagatgcgg aatcccagga agccgcgtgc gaaagcgcgc cgtagaagcg aaactccgcc
     1861 gtttcgaatt gggatggtga ggtccaaagg aggcgttggg cattcaacga cgcgtcgacg
     1921 gcggcggcat gatccccgga aaagaagcga gcctgcgcct ttcgggccca ataccagaac
     1981 tcaggcagac ccaacaccgg gttgctcgcc aggtggcgct caaatcgacg ttcatcaaac
     2041 ccttcatcgt tgaagcagcc gaatgtcgga gtcaacccac gcagcgttcg gataatctgg
     2101 acctgcgtag tgatgaggtc gaccacgagg ccgaagcgcg ctctccgggc aaactcgaga
     2161 ccattctcgg cttccggctg tacatcgcca agccgatctc ccactgtcag gaaattcgtg
     2221 atcaactcgt tccagctata tgccgcgaac gtaagatcac caatccggta cgcggcgtcg
     2281 aag
//
LOCUS       DASUOM010000350         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1460814,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000350 DASUOM010000000
VERSION     DASUOM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460814"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VFY27_07360"
     CDS             complement(<1..1377)
                     /locus_tag="VFY27_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5765369.1"
                     /translation="MALLLALAAVIVVRMRARLAQESHWRLAAAGALQESQDRFREFA
                     EAAADWLWEIDVEYRFTMDTGHAPRGGLQGSDLIGFRRWEMPGVDPEDPVWDRYRAML
                     DAREAFRNFEFSYTGQNGRLFHASINGHPLYGAEGKFNGYRGTARDITAEVEAKRHVA
                     RATALLDAVRLIQSSYIGGTDPKKACEQMLATLLDVTGSAYGFVGEIRHDETGKPYLK
                     AHAVTNIAWNAETHRLYDENAADGLEFRKLDTLFGAAITSGRPVIANSPATDPRRGGR
                     PEGHPPLNCFLGVPLYSGQDMVGLMGVANRAGGYDEAIVAFLEPLTGACGAVIAAIQA
                     DAQQAAVEAQLRATVGRLNAAESHAHLGSWEADLATGEMIASEEALDIFGQPQGIPVT
                     RQMFFNALVEEDRDQIKTTVDSLLASQATEYTIEYRIDHRERGVRRICANVAVERTVD
                     GAAVRLRGT"
     gene            1391..1543
                     /locus_tag="VFY27_07365"
     CDS             1391..1543
                     /locus_tag="VFY27_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765370.1"
                     /translation="MNHISGKAMILGVAARVGTAFASSDAGCAPALAVLPDLSCGLIE
                     FLLAVA"
     gene            complement(1713..2465)
                     /locus_tag="VFY27_07370"
     CDS             complement(1713..2465)
                     /locus_tag="VFY27_07370"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX5765371.1"
                     /translation="MKLVLLVLAAWIVVPVVVSCARYSAPGYSWSTAPRHSSGLAPDP
                     RIHSDGIVQIYNARAFSWRGIFATHPWIVVKKSGAPTYKRYEVVGWGSGSKLRTDYAT
                     VDGLWYGSTPSLLVDVRGPRADALIDRIEGAVASYPYTNDYRTWPGPNSNTFLAHVAR
                     EVPELSLDIPASAIGKDYRPWYQPIGYAPSRTGIQISILGLLGVTLAAEEGVEFNLLG
                     LAFGVDFLRPALRLPALGRLGMGEEVPEAPGQ"
     gene            complement(2603..>2842)
                     /locus_tag="VFY27_07375"
     CDS             complement(2603..>2842)
                     /locus_tag="VFY27_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765372.1"
                     /translation="DAQYLFATDVLGINRGHVPRHSKIYRNFAAEYARLQEERIAAFS
                     ELVQDIRSGAYPESRHQVGIPKIEFDQFLRAIEEE"
BASE COUNT          547 a          891 c          853 g          551 t
ORIGIN      
        1 ggtgccccgc agccgaacag cggcgccgtc gactgtgcgc tcgacagcga cgttcgcgca
       61 tatgcgccgg acgccacgct cccgatgatc aattcgatat tcgatggtgt actcggttgc
      121 ctggctcgcg agaagcgagt cgacagttgt cttgatctgg tcgcggtcct cctccacgag
      181 cgcgttgaag aacatttgtc gcgtaacggg aatgccctgc ggctggccga agatgtccaa
      241 agcctcctcg ctggcgatca tctcgccggt cgcgagatcg gcttcccatg agcccagatg
      301 cgcatggctt tctgcggcgt tgagccggcc gaccgtcgcg cgcaactgcg cttcaaccgc
      361 cgcctgctgc gcatccgcct ggattgcggc gatgaccgcg ccgcatgccc ctgtgagggg
      421 ttccagaaac gcgacgatgg cctcatcgta gccgccggct cgattggcga cgcccatcag
      481 gccaaccata tcctggccag aatagagcgg gacaccgagg aagcaattga gcggtggatg
      541 gccctctggc cgaccaccgc ggcgcggatc cgttgcagga ctgttggcga tcaccggcct
      601 gccgctggtg attgcggcac cgaacagggt atccagtttg cgaaactcca gcccgtccgc
      661 cgcattctcg tcatacaatc gatgggtttc cgcgttccag gcgatgttgg tgacggcgtg
      721 agccttgaga tagggcttgc cggtctcgtc atgccggatc tctcccacga agccatatgc
      781 gctcccggtg acatcgagca gcgttgccag catctgctcg catgccttct taggatcggt
      841 gcccccgata tagctcgact ggatcagccg aacggcatcg agcaaggctg tggcccgggc
      901 gacgtgcctc ttcgcttcga cttcggccgt aatgtcgcgc gccgttccgc gatagccatt
      961 gaattttccc tcggccccat agagcgggtg gccgttgatg ctggcgtgga acagcctgcc
     1021 attctgtcca gtgtaagaga attcgaaatt ccggaaggcc tcgcgcgcat caagcatcgc
     1081 ccgatagcga tcccagaccg ggtcctcggg atcgacaccc ggcatctccc agcggcgaaa
     1141 gccgatcagg tccgatcctt gcaagccacc gcggggcgca tgaccagtat ccatcgtgaa
     1201 gcggtactcg acgtcgattt cccaaagcca atcggctgcc gcctcagcga actcacgaaa
     1261 tcggtcctgg ctttcctgga gagcacctgc tgctgccaac cgccagtggg actcttgcgc
     1321 cagcctggcg cgcatgcgca cgacgataac cgccgccaag gcaagcagca gagccaagac
     1381 caaagcagcg atgaaccata tcagcggcaa agccatgatc cttggtgttg ccgcgcgagt
     1441 cggtaccgct ttcgcatcct cggatgcggg atgcgcccct gcgcttgcgg tgctgcccga
     1501 tctctcatgt ggccttatcg agttcctgct ggccgtggca taggccgcca gagcctcggc
     1561 agagcaactg gcattatgga atgcttatga ttaacagtgt gttgcagccg actgccgttg
     1621 cgaattgcaa ctaaaattgt aatactctga aatggctcgt aaatctgcga gaatgtagcg
     1681 caagtgggac cgggcgttct attccatccg tgttactgcc ctggagcctc cggaacttct
     1741 tcacccatcc caaggcgacc aagcgcaggc aggcgcagcg ctgggcgcaa gaaatccact
     1801 ccgaaagcca ggcccagcag gttgaactcc acgccttcct ccgccgccag ggtcacgccg
     1861 agcaacccga ggatcgagat ctgaatgcca gtcctgctcg gcgcatatcc gatcggctgg
     1921 taccatgggc gataatcctt gccgatcgcg ctggctggga tgtcgaggct caactccggc
     1981 acttcccgcg cgacatgggc caggaaggtg ttgctattgg gtcccggcca ggttcggtag
     2041 tcgttcgtat agggatagga ggcaactgcg ccttcgatgc gatcgatcag cgcatcggcc
     2101 ctcggaccgc gcacatcgac cagcaaggac ggcgtggagc cgtaccacag tccgtcgact
     2161 gtcgcgtaat cggtgcgcag cttcgagccg gaaccccaac ccacgacctc gtatcgtttg
     2221 taggtgggag cgccggactt cttgacgacg atccagggat gggtcgcaaa gatcccgcgc
     2281 caggaaaaag cacgggcgtt gtatatctgc acgattccat cactatgaat ccgcggatcc
     2341 ggggcaaggc ccgaggaatg ccgcggtgca gtgctccacg agtagcccgg cgccgaatag
     2401 cgtgcgcaac tcacaacgac cggaactacg atccaggccg ccagaaccag gaggaccagc
     2461 ttcatacgct gccttcgtgc cattcacccg aacgcctagg tagtgtcaca gcctacggtg
     2521 acgtgcaagg ctacgccagg gccgtgtaag gggcgtcggc tcacatatga ttgaagttct
     2581 gtggctgccc caacttgtgc cgtcactctt cctcgatcgc ccgcaggaac tggtcgaact
     2641 cgatttttgg gatgccgacc tgatgccgcg attcgggata ggcgccggat ctgatgtctt
     2701 gcaccagttc cgagaacgcg gcgatgcgtt cctcctggag ccgggcatat tctgctgcga
     2761 agttgcgata gatcttggaa tgccgcggca cgtgcccgcg attgatgccg agcacgtcgg
     2821 tcgcgaaaag gtattgggca tc
//
LOCUS       DASUOM010000351         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_452298,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000351 DASUOM010000000
VERSION     DASUOM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452298"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFY27_07380"
     CDS             <1..954
                     /locus_tag="VFY27_07380"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5765373.1"
                     /translation="VADALAVLLQQDVPLVEDWIDGAEAIPGQVVLLENVRFLKGEKA
                     SDDDLSRRMGALCDVFVMDAFGTAHRAQASTCGVARYAPVACAGPLLTAELEALDKAL
                     KDPARPVVAIVGGSKVSTKLTVLDALANIVDELIVGGGIANTFIAAAGHPVGKSLYEV
                     EMLDRAQALARNDRDRARIPVPVDVVVAKEFSASAKGEVRPIDAVNDDELILDIGPES
                     AAQFAGILSGAGTIIWNGPVGVFEFDQFGEGTRVIAEAIAESKAFSLAGGGDTLAAID
                     KYGVGDRISYISTGGGAFLEFVEGKTLPAVAILEERAAAAV"
     gene            1027..2460
                     /gene="pyk"
                     /locus_tag="VFY27_07385"
     CDS             1027..2460
                     /gene="pyk"
                     /locus_tag="VFY27_07385"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5765374.1"
                     /translation="MRRTKIVATLGTSTDDPERLRKMIRTGLDVARINFSHGTEEDQR
                     ARVERLRAAADASNKVVGIMGDLQGPKIRIQRFAEGKATLRDGDDFFLDTAMAPRTGT
                     SAGVGVAYADLPHDVTPGDVLLLDDGQITLSVERIEGSRIHAKVVNGGELSDHKGINR
                     QGGGLSAPALTEKDREDIRIAADLQVDFLAVSFVRSADDVNEARQLLREAGGQGLIVA
                     KIERTEAVHNIDQIIDAADVIMVARGDLGVEMGYAELTGMQKQIIRRTRRKNKIVITA
                     TQMMESMISHPIPTRAEVSDVANAVMDGTDAVMLSAETAVGAYPVGVIAAVNEVCVGA
                     EKYSLARGRTSHRLDDKFQAVDEAIAMAVMYTANHLAVKAIIALTESGSTTLWMSRIR
                     SDIPIYAFTPHDATRRRVTLYRGVYPISFKIEHSDAQLLYQDIFSTMLEHELVEVGDR
                     VIFTKGDLKGVSGSTNAMKIISVTSAL"
     gene            2491..3351
                     /locus_tag="VFY27_07390"
     CDS             2491..3351
                     /locus_tag="VFY27_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002049937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5765375.1"
                     /translation="MMANSRFTGTSSYVATHDLMMAVDAAVTLERPILIKGEPGTGKT
                     QLAVEVSRALDRPLYEWHIKSTTKAQQGLYDYDAVARLRDSQLGDDKVHDIANYIVRG
                     KIWEAFESDVQPVLLIDEIDKADIEFPNDLLQELDRMEFFVYETKQLVKARHRPIIII
                     TSNNEKELPDAFLRRCFFHYIRFPDKETMEKIVDVHFPGLKKSLLREALEAFYRVRDV
                     PGLKKKPSTSELLDWIKLLLAEDIPPEALRAEDARKAIPPLHGALLKNEQDVHLFEQL
                     LFLDRRGRTQ"
BASE COUNT          715 a         1015 c         1123 g          634 t
ORIGIN      
        1 gtggcggatg cgctggctgt cctgctgcaa caggatgtgc cgctggttga agactggatc
       61 gacggggccg aggcgattcc cgggcaggtg gtgctgctgg agaatgtgcg ctttctcaag
      121 ggggagaaag ccagcgacga cgacctgtca cggcgcatgg gcgctctttg cgacgtgttc
      181 gtgatggacg ccttcggtac ggctcaccgc gcccaggcca gcacctgcgg cgtggcgcgc
      241 tatgcacccg ttgcgtgcgc cgggccgctc ctgactgcgg aactggaagc gctggacaag
      301 gcgctgaagg atccggcaag gccggttgtc gccatcgtag gcggatcgaa ggtatcgacg
      361 aaactgacag tactcgatgc tctggcgaac atcgtcgacg agttgatcgt cggcggcgga
      421 atcgccaaca ccttcattgc tgctgccggc catcccgtcg gcaagtcgct ttacgaagtg
      481 gagatgctgg accgggcgca agcgcttgcc aggaatgaca gggatcgggc acgtatcccc
      541 gttcccgtgg acgtagtggt agcgaaggaa ttttcggcca gcgccaaggg cgaggttcgc
      601 ccgatagacg ccgtcaacga tgatgaactg atcctcgata tcggtcctga atccgccgca
      661 cagtttgccg gcatcctttc cggcgcggga acgatcatct ggaacggtcc ggttggcgtc
      721 ttcgagttcg accagttcgg cgagggcacc agggtcatcg ctgaagccat agctgaaagc
      781 aaggcctttt ccctggcagg cggcggcgac actctggccg ccatcgacaa gtacggcgtc
      841 ggcgaccgca tctcctatat ctcgaccgga ggaggcgctt tcctcgagtt cgtagaaggc
      901 aagacactcc ctgccgtcgc catcctcgaa gagcgagcgg ccgccgcagt gtaggagcag
      961 acgggaacag ggccggcgcg gagagtctcc ctcgcgtacc ccgttgtgca ataatccgcc
     1021 gctatgttgc gcagaaccaa aatcgtcgcc acgctgggca cctcgacgga cgacccggag
     1081 cgactcagga agatgattcg caccggcctc gatgtagcac gcatcaactt ctcgcacggt
     1141 acggaggagg atcagcgcgc gcgcgtggaa agactgcggg cggcggccga tgccagcaac
     1201 aaggtcgtcg gtatcatggg cgacctgcag ggcccgaaaa ttcgcataca gcgttttgcg
     1261 gagggcaagg cgacgctgcg cgacggcgac gactttttcc tcgacacggc gatggcgccg
     1321 cgcaccggta cttcggccgg cgtgggcgtc gcttatgccg acctgccgca tgatgtcaca
     1381 cccggtgatg tgctgttgct cgacgatggg cagatcacgc tctcggtcga gcggatcgaa
     1441 ggcagcagga tccatgcgaa agtggtgaac ggcggcgaac tgtccgatca caaaggcatc
     1501 aaccggcagg gcggcggcct ctccgctccg gcattgacgg agaaggatcg cgaagacata
     1561 cggatcgcgg cggacctgca ggtggatttc ctggcggtct cgttcgtccg cagcgcggac
     1621 gatgtcaacg aggcgcggca actgcttcgg gaagcaggcg ggcagggcct gatcgttgcg
     1681 aaaatagagc gtaccgaggc ggtgcacaac atcgaccaga tcatcgacgc tgccgatgtc
     1741 atcatggtcg cgcgcggcga ccttggtgtc gagatgggct atgccgagct gaccggaatg
     1801 cagaagcaga tcatccggcg gacccggagg aagaacaaga tcgtcatcac cgccacacag
     1861 atgatggagt cgatgatcag ccacccgatc ccgacccggg ccgaggtttc cgacgtcgcg
     1921 aatgcggtca tggatggtac cgacgcagtg atgttgtcgg cggagaccgc agtcggggct
     1981 tacccggtcg gcgtgatcgc ggctgtcaac gaagtctgcg tcggtgccga gaagtactcg
     2041 ctggcacggg gacgaacgtc gcatcggctc gacgacaagt tccaggctgt cgacgaggcg
     2101 atcgcgatgg cggtcatgta cacggccaat cacctggcgg tgaaagcaat catcgcgttg
     2161 acggaatccg gatcgaccac cctgtggatg tcgcgcatac gatcggacat cccgatttat
     2221 gcgtttacgc cacacgatgc cacgcgacgc cgggtgaccc tgtatcgcgg cgtctacccg
     2281 atctctttca agatcgagca ttcggacgcg cagctcctgt accaggacat tttttcgaca
     2341 atgctcgagc atgagctggt cgaggtcggc gaccgcgtga tcttcaccaa gggcgacctg
     2401 aagggcgtct ccggcagcac caacgcgatg aagatcatca gcgtcacgtc cgcgctctga
     2461 gttgcccggg ctcctcttgc atatggattg atgatggcga actcccgatt tacaggcacc
     2521 agttcctacg tcgcaaccca tgatttgatg atggcagtcg acgcagccgt aacgctcgag
     2581 cgacccatcc tgatcaaggg agaacccggc acgggcaaaa cgcagctggc tgtggaggtg
     2641 tcgagggcgc tcgaccggcc gctttacgaa tggcacatca agtcgacgac caaggcacag
     2701 cagggactgt acgattacga tgctgttgcg cggctgcggg attcgcagct gggcgacgac
     2761 aaggtgcacg atatcgccaa ctacatcgta cggggcaaga tctgggaagc attcgaatcc
     2821 gatgtgcagc ccgtactgct gatcgatgag atagacaagg ccgacatcga gtttccgaat
     2881 gatttgttgc aggagctgga ccggatggag ttcttcgtct acgagacgaa gcaactcgtc
     2941 aaggcgcgcc acaggccgat cattatcatc acgagcaaca acgagaagga gcttcccgac
     3001 gcgttcctgc ggcgctgctt tttccactac atccgcttcc cggacaagga gacgatggag
     3061 aagatcgttg atgtgcattt tccggggctg aagaagagcc tgctgcggga agcgctggag
     3121 gccttttacc gggttcggga tgtccccggc ctgaaaaaga aaccctcgac ttcggaactg
     3181 ctcgactgga tcaaactgct cctggcagag gacattccgc cggaggcact gcgcgccgag
     3241 gatgcgcgca aggcgattcc gccgctgcat ggtgcgctgc tgaagaacga acaggatgtt
     3301 cacctgttcg agcaactgct ttttctcgac cgccggggac gaacccagta atcgttcgat
     3361 ccatggatag tccggccaaa tgctgacggg gttctttttc aagctacggc tcggcggcct
     3421 gaaaccgtcc attaccgagt acctcaccct gctcgatgcg atgaacaggg gcctggccgc
     3481 gcggagc
//
LOCUS       DASUOM010000352         5930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_122619,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000352 DASUOM010000000
VERSION     DASUOM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122619"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1543)
                     /gene="mgtE"
                     /locus_tag="VFY27_07395"
     CDS             complement(197..1543)
                     /gene="mgtE"
                     /locus_tag="VFY27_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5765376.1"
                     /translation="MDPREAQSTLELLREELGSGGLRSARMLVGSLYPSELARLLESV
                     PPAERALIWEMVEPEREGDVLVELAEQVRDGLIRGMKTDQLVAATEGMELDDLADLLA
                     DLPEALTKEVMRSMDHQDRERLNQVLSYDEDTAGGLMNVDIVTVRPDVTLEVVLRFLR
                     ARGEIPDGTDMIYVVNRNNEYLGSLFLSRLLTEDPQQTVEQVMSSVTLPIPAHTSSSE
                     VVWEFENRDLLSAPVVDEDYRLVGRITVDDIVDLIREEAEHSLMSAAGLDEEDDMFAP
                     AITSARRRAIWLGVNLGTAFLAASVVDLFQTTIDKIVLLAVLMPVVPSMGGVAGSQSL
                     TIITRAIALGQIDRTNAGSIFRKEVLVGVLNGMLWASIVAVFTYFWFNDWHIGVVIAG
                     AMLINLFLAAAAGFAVPLLLKRMKIDPAIAGGVVLMAITDVAGYMSFLGLGTLLLL"
     gene            complement(1627..2529)
                     /gene="rapZ"
                     /locus_tag="VFY27_07400"
     CDS             complement(1627..2529)
                     /gene="rapZ"
                     /locus_tag="VFY27_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5765377.1"
                     /translation="MDTDAQDEKRLIIVSGLSGSGKSVALHALEDLGYYCIDNMPAAL
                     LKSLLNELSRNDDNSPVRVAVGVDARNRRQDLATLPQLIDDLRKNNVRTDFLFLQASD
                     DILLKRYSETRRRHPLDNHVTELRGAIEKERQLLGEVIDSADLIIDTSRTSVYELADA
                     IRKRVDSRRGNKLSVLIESFGYKNGIPADADFVFDLRCLPNPYWQQDLRSLTGQDEAV
                     AKFLEDQPLFMQMYDDILAFLTRWIPEYVSFDRSYLTIAIGCTGGQHRSVYMAEKLAK
                     ALKQTHEPVLTRHNELPQRGTDRK"
     gene            complement(2552..3052)
                     /locus_tag="VFY27_07405"
     CDS             complement(2552..3052)
                     /locus_tag="VFY27_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5765378.1"
                     /translation="MIFERILKPENVLCDVSARGKKHCLEILSELLANSSIGMPHEEI
                     FAKLAERERLGSTALGGGVALPHCRFAGVEETNAALLRLSEAVDFESPDGVPVDLVFG
                     MIVPEEITDSHRDHIATITEALKDAALQARLRSAGTPDALYSALISGHARAGNVPGQP
                     KQSLRS"
     gene            complement(3330..3650)
                     /gene="raiA"
                     /locus_tag="VFY27_07410"
     CDS             complement(3330..3650)
                     /gene="raiA"
                     /locus_tag="VFY27_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5765379.1"
                     /translation="MQLSLTGHHVDITSALRNYVESKVEKIGRHFDLVSDVHCILTVE
                     KLRHKAEATVNVNGGQIYADATEDDMYAAIDGLVDKLDRQVRKYKEKLADHHAREANK
                     RAFE"
     gene            complement(3822..5216)
                     /locus_tag="VFY27_07415"
     CDS             complement(3822..5216)
                     /locus_tag="VFY27_07415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5765380.1"
                     /translation="MMKPSLQLKLGQTLTMTPQLQQAIRLLQLPVLDLSAQIQDALEE
                     NVMLEMEDLPDVPKTDTEPTVEVETIKPEDTWQTNPMERIQESNWNGEGRPINEYADQ
                     SGQSLREHLLWQLELENFTPRESVIGEALIDSINDDGYLTTTLEEIVDYIGENSAVSV
                     EEIERTLAKVQRLDPGGIGARSLSECIILQLKQLDPSTPGLKLAIEIASNHLDAVGAQ
                     EYSELRRTLRTTEACLDDALALIKGCHPKPGQAVNTAAAEYVVPDVFVRKVDDRWQVE
                     ISSSGIPRLSVNQRYAQLLRGSTDHAALRTQLQEARWLVRSLEIRNETLMKVASCIVS
                     RQTEFLDHGDEAMKPLVLRDVAEEIGMHESTISRVTTNKYMHTPRGVFEFKYFFSSHL
                     ASADGSDQSSTSVRAKIRKLIGSENPSKPLSDSKIAKLLADDGISVARRTVAKYREAM
                     KISSSSERKVRKPR"
     gene            complement(5323..>5930)
                     /gene="lptB"
                     /locus_tag="VFY27_07420"
     CDS             complement(5323..>5930)
                     /gene="lptB"
                     /locus_tag="VFY27_07420"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5765381.1"
                     /translation="GKTTAFYMIVGLIPCDRGRILLDGHDLTALPMHRRAAMGLGYLP
                     QEASIFRKLTVEQNILAILENRSELDRAGRAKELDQLLDELHVGHVRTSLGISLSGGE
                     RRRVEIARALAAHPRFVLLDEPFAGVDPISVLDIQRIIRHLSDRSIGVLITDHNVRET
                     LGICGRAYILNDGSLIASGPPGEILENQHVREVYLGQEFQL"
BASE COUNT         1076 a         1788 c         1769 g         1297 t
ORIGIN      
        1 ctgggtgaag aaagacctga gcggcttcga cttgtaaggc attaaacagc agaccgtagg
       61 agcccgccat gcgggcgagt gcgatgtgcg tgatcccact tgcccgcatg gcgggctcct
      121 acaggtacca gtcgaaacaa cgctattggt tcaggcttcc gcaatatgag ctagtgcgct
      181 aatcgcaccc cgtacgctac agcagcagga gggtaccgag ccccaagaag gacatgtacc
      241 ccgcgacgtc ggttatcgcc atgagcacga cgccgcccgc aatcgccggg tcaatcttca
      301 tccgcttcaa gagaagcggc accgcgaaac cggccgccgc cgcaaggaac aggtttatca
      361 gcattgcgcc ggcgatcacg acgccgatat gccagtcgtt gaaccagaag taggtgaaga
      421 ccgcaacgat gctggcccag agcatgccgt tcaggacgcc caccagtacc tccttgcgga
      481 aaatgctgcc ggcgttggtc ctgtcgatct ggccgagcgc aatggcacgc gtgatgatcg
      541 tcagggactg gctgcccgca actccaccca tactgggcac gacgggcatc agcacagcga
      601 gcaggacgat cttgtcgatc gtcgtctgga aaaggtcgac gacggaagcc gcgagaaacg
      661 ccgtgccgag attcacccct agccagatcg cacgcctgcg cgcgctggta atcgccggcg
      721 cgaacatgtc atcctcttcg tcaaggcccg cggcactcat caacgagtgc tccgcctcct
      781 cgcgaatcag gtcgacgatg tcgtcgacgg taatccggcc gacaagccga tagtcctcgt
      841 cgacgaccgg tgccgacagc agatcacgat tttcgaattc ccacaccact tcggacgaac
      901 tcgtatgtgc gggtatcggc agcgtcaccg aagacatgac ctgttcgacc gtctgttgcg
      961 ggtcctccgt cagcaagcgg gacaggaaga gggagcccag gtactcattg ttgcggttca
     1021 cgacgtagat catgtccgtg ccgtccggta tttcgccgcg cgcgcgcagg aagcgcagca
     1081 cgacctcgag cgtcacatcc ggccgcacgg tcacgatgtc gacgttcatc aggccgccgg
     1141 ccgtgtcctc gtcgtaggag aggacctggt tcaggcgttc gcggtcctgg tggtccatcg
     1201 aacgcatgac ttcctttgtc agggcttccg gaaggtctgc caggaggtcc gcgagatcgt
     1261 cgagttccat gccttccgtg gcagcaacca gctggtcggt cttcatgccg cggatgagcc
     1321 cgtcgcgaac ctgttccgcg agctcgacca ggacgtcacc ctcacgttcc ggctcgacca
     1381 tctcccatat cagcgcgcgt tcggccggag ggacggattc gagcaggcga gcgagctcgg
     1441 aggggtaaag gctgccgact agcattcgtg ccgagcgcaa tccgccgctg cccagctctt
     1501 cgcgaagcaa ttcgagcgtg ctctgcgcct ctctcgggtc catcagattt ccctttgcat
     1561 cgccggcggt atccggccca gggcgcccgc ggaccgggcg attctacaag atgtgcggcg
     1621 gcgacgttat ttgcggtcgg tgccgcgttg cggcagttca ttgtgccgcg tcaggacggg
     1681 ctcatgcgtt tgtttcagcg ctttcgccag tttttccgcc atgtagaccg atcggtgctg
     1741 cccgcccgtg cagccgattg cgatggtgag gtaactgcga tcgaagctga cgtattccgg
     1801 tatccagcga gtcagaaagg cgaggatgtc gtcgtacatc tgcatgaaga gcggctggtc
     1861 ttccaggaat ttcgctaccg cctcatcctg tccggtcagg ctgcgcaggt cctgttgcca
     1921 gtaagggttt ggcaggcaac gcaggtcgaa gacgaagtcg gcgtcggcag gaatgccgtt
     1981 cttgtagccg aacgactcga tcagcaccga cagcttgttc ccgcgacggc tgtcgacccg
     2041 cttcctgatc gcatcggcca attcatacac gctggtccgc gacgtgtcga tgatcaggtc
     2101 ggccgaatct ataacctcac ccaggagctg ccgttctttt tcgatcgctc ccctgagttc
     2161 ggtaacgtga ttgtcgagcg gatgccggcg tcgagtctcg ctatagcgtt tcagcaggat
     2221 gtcgtcgctt gcctgcagga acaggaaatc cgttcgcaca ttgttcttgc gcaggtcgtc
     2281 gattagctgc ggcagtgttg ccaggtcctg ccggcggttg cgcgcgtcta ccccgacggc
     2341 gacacgtact ggtgagttat cgtcgttgcg gctcagttcg ttcagcaggg acttgaggag
     2401 agcagcgggc atgttgtcga tacagtagta gcccaggtcc tcgagtgcat gcagagccac
     2461 acttttcccg gagccggaca gcccgctgac gataatcagg cgcttctcat cctgtgcgtc
     2521 ggtatccata accttgatct ctgtgactcc tctaagaacg cagcgactgc tttggctgac
     2581 cggggacgtt gccggcacgg gcatgtccgg aaatcaatgc gctatacagc gcgtccggcg
     2641 tcccggcgct gcgcagccgc gcctgcaagg cagcatcctt cagcgcctcc gtgatcgttg
     2701 caatatggtc gcgatgactg tcggtgattt cctccggaac gatcatcccg aacaccaggt
     2761 ccacaggcac gccgtcggga gattcgaaat ccacggcttc agacagccgg agcagcgccg
     2821 cgttcgtctc ctcgaccccg gcaaaacggc aatgaggaag cgccacgcct ccgcccagcg
     2881 ccgtcgagcc caggcgctcg cgttcagcca gcttggcgaa tatctcttcg tgcggcatgc
     2941 caatgctcga gttggccagg agctcgctca gtatctcgag gcaatgcttt ttgccgcgcg
     3001 cgctgacgtc gcacagaaca ttttcgggtt tcaggattcg ttcgaaaatc atggtgaatt
     3061 actgctcagc cgtccttgaa gggaaacggc ttacaggggc cgttcggggg gtagctgccg
     3121 tacggtcggc gtgccggcgg cgaggtgcct gcgaaccgta cggcctagtg caccccgtcg
     3181 cagataatgc accattctcg cggcgctttt tcgcgtctgg cggcgttgca actcctcgca
     3241 atagcggtgc tattactcgt cgtcgcgcct tgccggccac gaaaaatcac tcgcgggaat
     3301 ggtgctatct acgacggggt acactgcctt tactcgaatg cgcgcttgtt tgcttctctc
     3361 gcgtgatggt cggcaagttt ttccttgtac ttcctgacct gccggtcgag cttgtccacg
     3421 aggccgtcga tggctgcgta catatcgtct tcggtggcgt ccgcgtatat ctgacccccg
     3481 ttgacgttga cagtggcttc agccttgtgc cgcagttttt ccaccgtcag gatacaatgt
     3541 acatcggata ccaggtcaaa atgccgtccg atcttttcga ctttactctc gacatagttc
     3601 cttagagcgg atgtgatatc gacgtgatgg cccgtgagac ttagttgcat ggcattctcc
     3661 tgtaatccct gacgccttga tccgcttaaa cccccgcctc gcgaatcgtt cgatcattat
     3721 gaacaatggt ttgcgtccag cctgtctgtg tcagcacctc ctcgccgcga atacggccat
     3781 ccgacccggg atcatatgta tggtcatgtc tatggtcatg cttatcgcgg cttacgcacc
     3841 ttgcgttcgc tcgaggacga gatcttcatc gcttcccggt acttcgctac ggtgcgcctt
     3901 gccacggaga tcccgtcatc cgcgagcagc ttcgcaatct tgctgtcgct gagtggtttt
     3961 gagggattct ccgagccgat gagcttgcga atcttcgccc gcacggacgt cgaagactgg
     4021 tcggagccgt ctgcggaagc aagatggctg gaaaagaaat acttgaactc aaaaacaccg
     4081 cgtggcgtgt gcatgtactt gtttgtggtc acgcgggaga tcgtcgactc gtgcatgccg
     4141 atctcctcgg caacgtcgcg caggaccagc ggcttcatcg cctcgtcgcc gtggtcgagg
     4201 aactccgttt ggcgggaaac gatgcagctc gcaaccttca tcaaggtttc gttacggatc
     4261 tcgaggctcc tgaccagcca gcgcgcttcc tgcagctggg ttcgcaacgc cgcgtgatcg
     4321 gtgctgccgc gcagcagctg ggcatagcgc tggttgaccg acagccgcgg aatcccggag
     4381 gagctgatct ccacctgcca gcgatcatct accttgcgga cgaaaacgtc tggcacaacg
     4441 tactcggcag ccgccgtgtt gaccgcctgt cccggcttcg gatggcagcc cttgatcagc
     4501 gccagtgcat catcgaggca ggcttccgtt gtacgcaaag tgcggcgtaa ctcgctgtac
     4561 tcctgggcgc cgacggcgtc gagatggttg ctggcaatct cgattgccag cttgaggccg
     4621 ggagtcgagg gatccagctg cttcagctgc aggatgatgc attcggacaa tgaccttgcg
     4681 ccgatgccgc ccgggtcgag gcgctggacc tttgccaggg tgcgttctat ctcctcgacg
     4741 ctgacggcgg agttttctcc gatatagtcg acgatctctt cgagcgtcgt cgtcaggtag
     4801 ccgtcgtcgt tgatgctgtc gatcagcgcc tcgccgatta cgctttcgcg aggtgtgaag
     4861 ttctcgagct ccagctgcca gagcagatgt tcgcgcagcg actggccgct ctggtcggcg
     4921 tactcgttga tcggccgccc ttcgccgttc cagttgcttt cctggatccg ctccatcgga
     4981 ttcgtctgcc aggtatcttc cggcttgatc gtctccactt ccacagtcgg ttcggtgtcg
     5041 gttttcggga cgtccggaag atcctccatc tcgagcatca cgttttcttc gagcgcgtcc
     5101 tgtatctgcg cactgaggtc gagtaccgga agctgcagca ggcggatggc ctgttgcagt
     5161 tgaggagtca tcgtgagcgt ttgcccgagc ttgagttgaa gcgaaggttt catcatgcgg
     5221 aaagtttttc caggtctggc gcagtgaccg gctttgcagg cgcggtgcgt cgactgacgg
     5281 gcgctaccgt catgatatag gttgtgtggt gccgattgtg gttcagagtt ggaactcctg
     5341 tccgagatac acttcccgaa cgtgttgatt ctccaggatc tcgccgggcg gtccggaagc
     5401 gatcaggcta ccgtcattga gtatataggc tctgccgcaa attccgaggg tttcccttac
     5461 attatggtca gtaatgagga cgccgatgct gcgatcactc aagtgcctga ttatgcgctg
     5521 aatatcgagc acggaaatgg gatctacgcc agcgaacggt tcgtccagca gcacgaacct
     5581 cgggtgggcg gccagggcac gggcaatctc tacccggcgc cgttcgccgc ccgacaggct
     5641 gatgccgagg ctggtcctga catggccaac gtgcagttcg tcgagcagct gatccagctc
     5701 cttcgctcgc cccgcgcggt ccagttcgct ccggttttcc aggatggcca gtatgttctg
     5761 ttcaaccgtc agcttacgga agatcgatgc ttcctgcggc aggtaaccga gtcccatggc
     5821 agcgcggcga tgcatcggaa gcgccgtgag atcgtgacca tccaggagga tccttcccct
     5881 gtcgcaggga atcagcccga caatcatgta aaaggcggtc gttttgccgg
//
LOCUS       DASUOM010000353         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_978982,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000353 DASUOM010000000
VERSION     DASUOM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978982"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="VFY27_07425"
     CDS             complement(<1..88)
                     /locus_tag="VFY27_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5765382.1"
                     /translation="MRLFGPLYERVLEWSRHRHAERYLAGLSF"
     gene            complement(163..831)
                     /locus_tag="VFY27_07430"
     CDS             complement(163..831)
                     /locus_tag="VFY27_07430"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5765383.1"
                     /translation="MNRHSRARGIGMTSARTRDRLVERLAAQGIRSRVVLERIRNVPR
                     HLFVDEALESRAYEDTALPIGQGQTISQPYVVARMTESLLEGFGGERVLEIGTGCGYQ
                     TAVLAPLVKEIFTVERIPELLRRTRQRLRDLDLYNVRFRLGDGWEGWRKYSPYDAIIV
                     AAAAPELPPKLLEQLAPGGRLIIPVGPPRRQELCRVTRADGDFERASLGAVSFVPLVQ
                     GKKF"
     gene            complement(831..1574)
                     /gene="surE"
                     /locus_tag="VFY27_07435"
     CDS             complement(831..1574)
                     /gene="surE"
                     /locus_tag="VFY27_07435"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5765384.1"
                     /translation="MKILVSNDDGYLATGINALAAALSKVAEIVVVAPERNHSGASNS
                     LTLHSPLRIRKVAEDHYFVDGTPSDCVHLALSGFLDFEPDIVVSGVNHGANLGDDVIY
                     SGTVAAAMEGRFLGFPAIAVSLVGHRAVHFDTAARVACELVERLQRDPAPKDVILNVN
                     VPDRPYAELTGIVAARLGFRHRSEPLIESVDPQGSKIYWIGPAGPGQDAGPGTDFAAI
                     EAGAVSVTPLKVDLTRHEALDRVSDWLSR"
     gene            1775..2341
                     /locus_tag="VFY27_07440"
     CDS             1775..2341
                     /locus_tag="VFY27_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5765385.1"
                     /translation="MPRRKNQWEKSSGHKGADDEGQLFRRAMADARPIRTDTVEPQRR
                     RTRAVARFSRLDERQALRESLEADPHAMEIENGSALWYRHPSVNLKTMRRLTRGGVSV
                     QACVDLHGMNSDEARRALSGFIEQSMVRGHTCIRVVHGKGLGSGHKGPVLKRKVDQWL
                     RRWEPVLAFVSARQADGGTGAAYVLLRK"
     gene            complement(2327..>2783)
                     /gene="truD"
                     /locus_tag="VFY27_07445"
     CDS             complement(2327..>2783)
                     /gene="truD"
                     /locus_tag="VFY27_07445"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX5765386.1"
                     /translation="IALSAARSFLFNLVLERRVAEQTWNTLLPGDCANLDGSGSVFPV
                     DAVDDVLLQRAATLDLHPTGPLAGSGERIVSGAVAILEESTIAGYREIVNGLESHRVS
                     HSRRALRARVSELKWQQPDEQTLWLEFTLGRGTFATSLLRELCRALLSQ"
BASE COUNT          541 a          875 c          878 g          489 t
ORIGIN      
        1 cgaatgaaag tccggcgaga taccgttcgg cgtgacggtg ccgcgaccat tccagtacgc
       61 gctcgtacag gggaccgaag aggcgcattc agcccaggct ccgcaacgca aagggaactt
      121 gcgagtttac gtcgtgatgt aatcgtactg tgttgtaaat actcaaaatt tctttccttg
      181 tacgagcggc acaaaactga ctgcaccaag cgatgctcgt tcgaaatcgc cgtctgcgcg
      241 ggtgacgcgg cacagttcct gtcgccgcgg cggtcccacc gggatgatca ggcgcccccc
      301 gggggccagt tgttccagca gtttgggcgg cagctccggc gccgccgcag cgacaatgat
      361 ggcgtcgtat ggactgtact tgcgccaccc ttcccagcca tccccgagcc tgaagcgtac
      421 attgtataaa tcgagatcgc gcaggcgctg ccgagtcctt ctcaaaagct caggaattcg
      481 ctcgaccgtg aaaatttctt ttacgagcgg tgcgagcacc gcagtctggt agccgcatcc
      541 ggtgccgatt tccagcaccc gctcgccccc gaaaccctcg agaagcgatt cggtcatgcg
      601 ggcgacgacg tagggctggc tgatggtctg gccctgcccg atcggcagcg cggtgtcctc
      661 gtaagcccgg ctttcgagcg cttcgtcgac gaacaggtgc cgcgggacat tacggatgcg
      721 ctcgagaacc acccgggatc ggatgccttg cgcggcaagc cgttcgacca ggcgatcgcg
      781 cgttcgcgcg gaggtcatgc cgataccgcg ggcgcgggaa tgccggttca ttaacggctc
      841 agccagtcgg agaccctgtc cagcgcttcg tgccgcgtca ggtcgacttt gagcggcgtg
      901 accgacaccg cgccggcttc gatggcggcg aagtcggttc cgggtccggc atcctggccc
      961 ggtccggcag gacctatcca gtagatcttg cttccctgtg gatcgacaga ctcgatcagc
     1021 ggttccgacc ggtgcctgaa ccccagacgc gcggcaacaa tcccggtgag ttcggcgtaa
     1081 ggcctgtccg ggacgttcac gttcaggata acgtctttgg gcgccgggtc tcgctgcagg
     1141 cgttccacca gctcgcaggc gacccgcgcc gcggtgtcga aatgcaccgc gcgatgaccg
     1201 accagggaca ccgcgatcgc cggaaacccg aggaagcgcc cttccatcgc ggcggccaca
     1261 gtgcctgagt agatgacgtc atcgcccagg ttcgctccgt ggttgacccc cgaaaccacg
     1321 atatccggct cgaagtcgag gaaacccgac agcgcaagat gcacgcagtc ggatggcgta
     1381 ccgtcgacga aataatgatc ttccgcaacc ttccggatgc gaagaggcga atgcagcgtg
     1441 agggaattac tggccccgct gtggtttcgt tcaggcgcca cgaccacgat ctcggccacc
     1501 ttcgacaacg cggcggcaag cgcgttgatt ccggtagcca ggtacccgtc gtcattgctg
     1561 accagaattt tcaattgcct tccctgttga cactgcgaat gatagccccc accttggccc
     1621 tgcaaaccgg ttttgcggtg ataagctatc acggaccatg ctccggctgg cgcccgcgga
     1681 cggagcggct gcgaccctga aaaatcggcg cacactatac tgaatgttgt cacgcgaccc
     1741 tagtgcacca atatcgcgat cacgcggagc cagcatgcct cgacgaaaga accagtggga
     1801 aaaatcgagc ggccataaag gtgcggacga cgagggccag ttgttccggc gcgcaatggc
     1861 tgatgcaagg ccgatacgca cggacactgt cgagccccaa cggcgaagga cccgggcggt
     1921 cgcacgattc tcgcggctcg atgaacggca ggcgctccgc gaaagcctcg aagccgatcc
     1981 tcacgctatg gagatcgaga atggcagcgc gctctggtac cggcacccgt cggtcaattt
     2041 gaagaccatg cgccgcctga cgcgaggcgg cgtcagcgtc caggcctgcg tggatctgca
     2101 tggcatgaat tcggacgagg ccaggagggc tttgagcggg ttcatcgaac agtcgatggt
     2161 ccgcggccat acctgcatcc gcgtcgtgca cggcaaaggt ttgggctcag gacacaaggg
     2221 accggtcctg aagcgcaagg tcgaccagtg gctgcggcgc tgggagccgg tactggcgtt
     2281 cgtatccgct cgacaggctg acggcggcac cggcgcagcg tacgtgttat tgagaaagta
     2341 gcgcccggca aagctcccgc agcagcgagg tggcgaaggt gccgcggccg agggtgaact
     2401 cgagccacag ggtttgctcg tcgggctgct gccatttcag ctccgagacg cgtgcacgaa
     2461 gcgcgcgccg cgaatggctg acgcggtgcg actcgagtcc gttcactatt tcccggtaac
     2521 cggcgatcgt cgactcctcg aggatcgcga ccgcgccgga cacgatccgc tctccgctac
     2581 ccgcgagcgg ccccgtagga tgcaggtcca gcgtcgcggc acgttgcagc aatacgtcat
     2641 cgacggcgtc gaccggaaat acgctgccgc tgccgtcgag gttcgcgcag tcgcccggta
     2701 gcagggtatt ccatgtttgt tccgccaccc tgcgttccaa aacaagattg aacaggaaag
     2761 agcgtgccgc cgacagggcg atc
//
LOCUS       DASUOM010000354         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_230058,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000354 DASUOM010000000
VERSION     DASUOM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230058"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="VFY27_07450"
     CDS             complement(<1..1821)
                     /locus_tag="VFY27_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765387.1"
                     /translation="MQVLPAIVLLCLLFASAGSAAQGTGQGDETFDIAIPEQPLAAAL
                     QSLAEQIDVQIVFFSGATEGLSARPLSGSFTRLEALNALLAGTGLRYVFLNADSVAIQ
                     PVPETEQHAAASPSPADAPRPPPAEARQFLPRESPTRSFAMEEVVVTGTASRSRTKYE
                     SSVGISTFDRVDIDRHLPTSTADLISAVPGFWVESTAGTTHGNVFARGIVQDGGYRYV
                     GLIEDGLPVYPVFELSFYNPDQFIRLSESTARVEVVRGGTAPVFTSGAVGGTINFVNE
                     EVSANPGLRMKAGYSDFDTRALDAHWNASLSDTWHASAGGYLRRSDGVRNPGYVADRG
                     GQLRLALQRTTPGSELEVYAKYLDDRSLFVVPVPLRGRPSNPSAVDGSAAGEYSLHSE
                     DLRNAGLPPSAREVGLSGSDLSDGIHPGLLTAGLRMDFMWDSGMSLTTHSRLTSGDVS
                     FNAIFTGDVPKTGLQFAAERNVAPDYSVISSGEAFDPAFLVQNHGHWAIAKDYRALQN
                     DTRLNFVVSEHDLTVGGYFADFSMNDRWSLGNLLLMDVGDQPQRLHLEGITDPRGFTQ
                     YSFLNLLADYDGRMSALYLADEWSVSDRLRVDLGLRMDSAS"
     gene            complement(1849..>2871)
                     /locus_tag="VFY27_07455"
     CDS             complement(1849..>2871)
                     /locus_tag="VFY27_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5765388.1"
                     /translation="SRIDTAPSISEQAADWLVRLSESAVSEPHRRQFMEWLKKSPQNV
                     EEFIAIANLHQEVGEQKSSLAEILAEVGCRNHQPAVPLFGGAASTGELPARARPQRRY
                     YQLWTTAAGLALLAVLLLQWFPRDPPLVSHRTQLGEQRSVVLADGSIITLNTLSEAAV
                     RFDRSAREVTLVSGEAMFDVVSDPGRPFIVQTGSVSLKVIGTRFAVYRKKNSTRVAVV
                     DGVVQALPLRTRGEPLFVEAGMGAIATVEGVTLTDDQFDVEKAIAWTDRRLIFDGAPL
                     AEVVAEFNRYNRTPLIVEDSALANRAITTVFNAHDVSALVAFLELEPDVEVQYGEDAI
                     RIRVSR"
BASE COUNT          575 a          886 c          902 g          508 t
ORIGIN      
        1 agaagccgaa tccatgcgca gcccgaggtc gaccctgagc ctgtcgctca cgctccattc
       61 atccgcgagg tacaacgccg acatcctgcc gtcgtagtcg gccagcaggt tcaggaacga
      121 atactgggtg aacccgcgag ggtcggtaat accctcaagg tgcaggcgtt gcggctggtc
      181 gcctacatcc atcagcagca ggttgccgag actccagcgg tcgttcatcg agaaatccgc
      241 aaagtagccg cccaccgtca ggtcgtgctc ggataccacg aaattgagtc gcgtgtcgtt
      301 ctgtaaagcg cggtagtctt tcgcgatcgc ccaatgcccg tgattctgga ccaggaacgc
      361 gggatcgaat gcttcaccac tgctgatcac ggagtaatca ggagcgacgt tgcgttccgc
      421 ggcgaattgc aggcccgtct tcggcacgtc gccggtgaag atggcgttga aagagacatc
      481 gccgctggtc agccggctgt gggtggtgag cgacatgccg gaatcccaca tgaagtccat
      541 gcgcaagcct gccgtcagca ggcctggatg gattccgtct gacaaatcgg aaccagagag
      601 gccaacctcg cgtgcgctgg gtggaagtcc cgcatttctc aagtcttcgc tgtgcaggga
      661 atactcgccg gccgcagacc cgtcgacagc cgaaggattg gatggcctgc cgcgcaacgg
      721 aacaggcacg acgaacagcg aacggtcgtc gagatacttt gcatacacct cgagttccga
      781 cccgggtgtc gtgcgctgca gcgccaggcg caactggcct ccccgatcgg caacgtagcc
      841 agggttgcgt acgccgtccg atctccgaag atagccgcct gcggatgcgt gccaggtatc
      901 cgagagcgac gcgttccagt gagcatcaag cgcacgcgta tcgaagtccg agtagccggc
      961 tttcatgcgc agcccgggat tcgccgagac ctcttcattc acgaaattga tcgtgccgcc
     1021 gacggcgccc gaggtgaata ccggggccgt gccgccgcgg accacctcca cccgcgcggt
     1081 ggactcgctg agccggatga actggtcagg gttatagaac gaaagctcga acaccgggta
     1141 tacggggagc ccgtcttcga tcagcccgac gtaacgatag ccgccatcct gcacgattcc
     1201 gcgagcgaag acgttgccat gggtggttcc cgcggtggat tcgacccaga agccgggaac
     1261 cgccgatatc agatccgcgg tgctggtggg caggtgccgg tcgatatcaa cacgatcgaa
     1321 tgtcgagata ccgacgctcg actcatactt cgtgcgactg cgggacgcgg taccggtaac
     1381 gacgacctcc tccatcgcga aggaccgcgt cggcgactcg cgcggcagga attgccgtgc
     1441 ttcggcgggc ggcggacgtg gggcgtcggc aggcgaaggt gatgccgctg catgttgctc
     1501 cgtctccggg accggctgaa tggccaccga gtcggcgttc aggaaaacgt agcgaagccc
     1561 ggtaccggcc agaagcgcgt taagtgcctc cagccgggtg aatgagccgg agagaggccg
     1621 ggcggacagt ccctcggtag cgccggagaa aaatacgatc tgaacgtcga tctgttcagc
     1681 aagcgactgc agggcggcgg caagcggctg ctccggaatc gctatgtcga aggtttcatc
     1741 cccctgcccg gtcccctggg cggcactacc ggcagaagcg aacagcaggc aaaggagaac
     1801 gatcgccggc agcacctgca tcagttggga agctccatcg cgtgacgttc aacgactgac
     1861 acggatccgg atcgcgtctt caccgtactg gacttcaacg tccggttcga gttccaggaa
     1921 ggcgaccagc gcgctgacgt cgtgcgcatt gaataccgtg gtgattgccc gattcgccag
     1981 cgcgctgtct tccacgatca atggcgtgcg gttgtagcgg ttgaattcgg cgacgacctc
     2041 ggcgagagga gcgccatcga atatgaggcg ccggtcggtc caggcgatcg ctttctccac
     2101 gtcgaactgg tcatccgtga gagtcacgcc ctctacggtc gcaatggcgc ccatgcccgc
     2161 ttcgacgaac aacggctccc cgcgggtgcg aagcggcagc gcctggacaa caccatccac
     2221 gaccgcaacg cgcgtggagt tcttcttgcg gtataccgcg aacctcgtgc cgatcacctt
     2281 cagggagacc gatccggtct gcacgatgaa aggacgtcca ggatcgctga cgacatcgaa
     2341 catggcctcg ccggaaacca gcgtcacctc cctggcagag cggtcgaaac gcacggccgc
     2401 ctcggacaac gtgttgagcg tgatgattga gccgtcggcc agcaccacgg aacgctgttc
     2461 gcccagttgc gttctatgac tgacgagcgg cggatcgcgt ggaaaccact gcagcaggag
     2521 gaccgccagc agcgccagtc ccgccgctgt ggtccacagc tggtagtagc gtcgctgcgg
     2581 ccttgcccgc gctggtaatt cgccggttga cgctgcgcca ccgaacaatg gcaccgccgg
     2641 ctgatgattg cgacagccca cttcggcgag tatttcggct aacgagctct tctgctctcc
     2701 tacctcctga tgcaagttcg cgatcgcaat aaactcctcc acgttctgcg gagatttctt
     2761 cagccactcc atgaactgtc ggcggtgagg ttcgctgacg gcagattcgg aaagccggac
     2821 cagccagtct gcagcttgtt cggaaatgga gggggcagta tcgatgcgtg a
//
LOCUS       DASUOM010000355         5201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_523446,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000355 DASUOM010000000
VERSION     DASUOM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523446"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFY27_07460"
     CDS             <1..1330
                     /locus_tag="VFY27_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5765389.1"
                     /translation="TDEEDDADSGGYEAPGEGVSLTRRVEADKPSGLVALTGARIITM
                     TDADGGVIEDGVIVTDGNRIRAVGAGSDVDIPGSARRVDVSGKTIIPGIIDAHAHGPQ
                     GVGIIPQQNWAAHATLAFGVTTVHDPSNDATEVFAASEMQRAGLILAPRIFSTGDIVY
                     GAKSTYFAEIDSLDDARQHVRRLKAQGAMSVKNYNQPRRSQRQQVVVAAREENMLVVP
                     EGGSLYHMDLSMVADGNSSVEHNLPQSMLYDDVLQFWGQTEVAYTPTLVVTYGGLAGE
                     TWWYQQTDVWKHPILSNFVPPQILRPRSVRRLKAPESEYHHAQSAATARLLADEGVLV
                     SIGAHGQREGLASHWEIWSFVQGGMTPVEALRAATASPARALGFDRDLGTLEEGKLAD
                     LVVIDADILDDIFRSDRVSMVMLNGRLYDAATLDETVTGDHETEPFFWQR"
     gene            1437..2303
                     /locus_tag="VFY27_07465"
     CDS             1437..2303
                     /locus_tag="VFY27_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5765390.1"
                     /translation="MKKVTAVQSAPGQHWVGDGFPVRSLFSYQTHANALSPFLLLDYA
                     GPRDFVAAERPRGVGQHPHRGFETVTIVYQGEVEHRDSTGGGGTIGPGEVQWMTAGGG
                     LLHEEFHSPDFTRKGGTFEVVQLWVNLPARNKMIEPAYQAIGRDRIPQVMLPDDAGTL
                     RIIAGEYDGHEGPARTYSPMHVWDMHIRRDAAAAFEIPEGWTTAIVVLHGTVLVNETE
                     LVREAQFALLDRSGTTVSIEANNDAKLLLLAGEPIEEPVVGYGPFVMNSREQIQQAIE
                     DFNSGRFGSMPG"
     gene            complement(2344..2910)
                     /locus_tag="VFY27_07470"
     CDS             complement(2344..2910)
                     /locus_tag="VFY27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5765391.1"
                     /translation="MIVYAAISFVLLAGFFLGYVSTRPDTFRVQRSAVIDVPPAQVFP
                     FIEDFRQFALWSPYEKLDPGMTRTFSGPKRGKGAVYEWHGKAAAGKGRMEIAHTSPPH
                     MAVIRLDFVKPFKTQNIVEFTLREEGSATRVTWAMRGANPYIAKMIQLLFNMDRMVGR
                     DFETGLLNLKALVEGQAEEVLPRLASAG"
     gene            3189..4778
                     /locus_tag="VFY27_07475"
     CDS             3189..4778
                     /locus_tag="VFY27_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5765392.1"
                     /translation="MARRYAMKYGCARFLTGTLCLALAACGGGGGGDSGGGDGGGSWT
                     PGLFLPSSSFAAQCSAPRSGIDPETGQPYPDLQGSVLDENNFLRSWSDETYLWYDEIA
                     DQDPAGFNDPLVYFDDLRTFATTPSGADKDKFHFTYPTDEWFELSQSGVSAGYGAAWV
                     ILAPVPPREAVVAYIEPNSPAAAANLARGARVLSVDGAQVLDGPPAILNAGLFPESDG
                     ETHTFVVEDLGSGGNTRSITMTSEKVTAAPVQNVKTVASPIGTVGYLLFNDHIATAEE
                     ALIDAVEQLASAGIDELVLDIRYNGGGYLAIASQVAYMIAGDARTAGQTFEEIQFNDK
                     HPSTNPVTGQPLQPYPFLDTTAGFSSLPSGTPLPALDLERVVVITGPNTCSASESIMN
                     SLRGVDVEVIQIGSTTCGKPYGFYPTDNCGTTYFTIQFRGVNDKGFGDYTDGFSPFNT
                     ALVEGELVPGCSVADDFENALGDPLEKRFQSALAYLETGSCPTPSGVTAPGLSKTKAL
                     LSATDGLMPKSPWRENRILRR"
     gene            4782..5159
                     /locus_tag="VFY27_07480"
     CDS             4782..5159
                     /locus_tag="VFY27_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765393.1"
                     /translation="MNSAKVWLILLFTMSLAGCNLNGVHDERPALIVAPTAESRAELE
                     RAVSEMLHGADVMLADDALTASSILVVERNRIRSMENRPLSGRDLGRPERFRLVTTGD
                     RCVLIHENDDARRELLETECVPE"
BASE COUNT         1040 a         1595 c         1619 g          947 t
ORIGIN      
        1 cactgacgag gaggacgatg ccgatagcgg cggctacgag gcgccgggag aaggagtctc
       61 gctgacgcgt cgcgtcgaag cggacaagcc atccggactg gttgccctga ccggtgcgcg
      121 gatcattacg atgacggacg ccgacggtgg agtcatcgag gatggtgtca tcgtcacgga
      181 cgggaatcgt atccgtgcag tcggtgccgg gagcgacgtc gatatccccg gttctgccag
      241 gcgcgtggac gtaagcggca aaacgataat cccggggatc atcgacgcgc atgctcacgg
      301 accgcaagga gtcgggatca tcccgcagca gaattgggct gcgcacgcga cgctggcgtt
      361 cggcgttaca actgtgcatg acccgtcgaa cgacgcgacc gaggtgttcg cagcttccga
      421 gatgcaacgt gcgggcctca tcttggcgcc gcggatcttt tccacgggcg acatcgtcta
      481 cggcgcgaag tctacctact tcgccgaaat cgattcgctg gacgatgcgc gccagcacgt
      541 caggcggctg aaggcacagg gcgcgatgag cgtgaaaaac tacaaccagc cacggcgctc
      601 gcagcggcag caggttgtcg tggcggcacg tgaagagaac atgctggtag tgccggaagg
      661 cggatcgctc taccacatgg acctgtccat ggtggccgac ggcaacagca gcgtggaaca
      721 caacctgccg caatcgatgc tgtacgacga cgtgctgcag ttctggggcc agacggaggt
      781 tgcgtacacg ccaaccctcg tcgtcaccta cggcggcctc gccggagaaa cctggtggta
      841 ccagcagacc gacgtctgga agcacccgat actgtcgaac ttcgtaccgc cacagatcct
      901 gcgtccgcgg tcggtgcgaa ggctgaaagc tccggagagc gagtatcacc atgcacagag
      961 tgcggctacg gccaggttgc tggccgacga gggagtcctc gtcagcatcg gcgctcacgg
     1021 gcagcgggaa ggtctcgcca gtcactggga aatctggagt ttcgtccagg gaggcatgac
     1081 gcctgtcgaa gcgctgcggg cggccacggc ctcaccggcg cgcgcactcg gcttcgaccg
     1141 ggatctcggc actctggagg aaggcaagct tgcagacctg gtagtgatag acgccgatat
     1201 cctcgatgac attttcagga gcgaccgtgt ctcgatggtc atgctgaacg gccgcctcta
     1261 tgacgcagca acgctcgacg aaaccgtcac cggcgaccac gagaccgagc cgttcttctg
     1321 gcagcgttag caccgtgccg gtgcgacgca cgcatggcgg gcccgcgcgg aaccatccat
     1381 cttgaaaagg cccggaccgg cacagatatc gccgtatcgc tgttgaggat gaatcgatga
     1441 aaaaggtcac agccgtgcaa agcgccccgg gacagcactg ggtcggcgac ggttttcccg
     1501 tccgctcgct tttctcctac cagactcacg ctaacgcact gagcccgttc ctgctgctgg
     1561 actatgcggg ccccagggac tttgtggcag ccgagcggcc acgcggcgtc gggcagcatc
     1621 cgcaccgggg ctttgaaaca gtgacgatcg tttaccaggg tgaggtcgag catcgcgatt
     1681 ccacaggtgg cggcggcacg atcggacccg gcgaagtcca gtggatgacc gcgggcggcg
     1741 gactgctgca cgaggagttt cattcgcctg atttcacgcg gaagggtggc acgttcgagg
     1801 tcgtgcagtt gtgggtcaac ctaccggccc ggaacaagat gatcgaaccc gcttaccagg
     1861 cgataggccg tgacaggatt ccgcaagtca tgcttcccga cgacgccggg actttgcgga
     1921 ttatcgccgg tgagtacgac ggccatgaag gcccggcgcg cacttacagt cccatgcacg
     1981 tctgggacat gcacattcgc cgggatgcgg ccgcggcgtt cgaaattcct gaaggatgga
     2041 caaccgcgat cgtcgtactg cacggcaccg tgctggtgaa cgaaaccgag ctcgtgcgcg
     2101 aggcacagtt tgcgctgctc gatcgttcag gcactaccgt ttcgatcgag gcaaacaacg
     2161 acgcgaagtt gctgctgctt gccggtgagc ctatcgaaga accggtcgtc ggttacggtc
     2221 cgttcgtcat gaacagcagg gagcagatcc agcaggcgat agaggatttc aacagcggcc
     2281 gcttcggcag catgccgggc tgactcgcct gtgccaggat gggcaccggc agtacgcgga
     2341 acatcagccc gccgatgcaa gccgcgggag gacctcttcg gcctgtccct cgacaagcgc
     2401 cttcaggttc agaagcccgg tttcgaaatc cctcccgacc atgcgatcca tgttgaacag
     2461 caactggatc atcttggcaa tgtacggatt cgcgcctcgc atcgcccagg tcacccgggt
     2521 tgccgagccc tcctctcgca gcgtgaattc cacgatattt tgcgttttga acggcttgac
     2581 gaagtcgagt cggatgaccg ccatgtgagg cggtgaggtg tgcgcaattt ccatgcgtcc
     2641 cttgccggcg gccgccttgc cgtgccattc gtaaactgcg cccttgcctc gtttcggccc
     2701 gctgaaagtc ctcgtcatcc ccgggtcaag cttttcataa ggcgaccaga gtgcgaattg
     2761 acgaaagtct tcgataaacg gaaaaacttg cgcaggcggc acatcgatga cggctgaacg
     2821 ctgcacgcgg aacgtatccg gacgcgtcga cacatagccg aggaagaatc ccgccaacag
     2881 aacgaatgaa atggctgcgt atacgatcat gactgttctc cgtgttcctg ttcgaagccc
     2941 ggatctccgg ccgcaggttt ctgttttcag gcctttccgc cgttgcgtgt ccgtgcttac
     3001 taagatgtcg aacgagcgcc gacggtttcg acgcaggagt gattattttt gctgtcgcca
     3061 tgccgggaca gacgggagat ccgtgttgag agatttgagt tggcagacgg aaagcaagct
     3121 tacgtggtta aatcggccat ctcggactgt atgatctgcg gcgtgccgca ggcgggggaa
     3181 cgttcaaaat ggccagacgc tacgctatga aatacggttg cgctcgattc ctcaccggca
     3241 cgctttgcct cgctctcgcc gcctgcggcg gcggcggtgg cggagatagt ggcggcggag
     3301 acggcggcgg tagctggacg cccggtctct tccttccctc aagctccttt gcggcccagt
     3361 gcagcgcacc gcgcagcggc atcgatcccg agaccggcca gccatatccc gacctgcagg
     3421 gcagcgtcct cgacgagaac aacttcctca ggtcctggag tgacgaaacc tatttgtggt
     3481 acgacgaaat agccgaccag gatccggccg gcttcaacga ccctcttgtt tacttcgacg
     3541 acctgagaac gttcgccacg acgccttccg gagcggacaa ggacaagttt catttcacct
     3601 atcccaccga cgaatggttt gagctttcgc agtccggcgt gtccgcggga tatggcgcag
     3661 cctgggtcat tcttgccccc gttcccccaa gagaagcagt tgtcgcctat atcgagccga
     3721 attcgccagc tgcggcggct aatcttgccc gcggtgcgcg ggtgctttca gtggatggtg
     3781 cgcaggtcct cgatggacct cccgcaatcc tgaacgccgg cctgttcccg gagtccgacg
     3841 gagagacgca tacgttcgtc gtcgaagacc tgggctccgg aggaaacacc cgctcaatca
     3901 cgatgacgtc ggagaaggtc acggcggcac cggtgcagaa cgtcaagacg gtcgcctcgc
     3961 ccatcggtac ggtcggttac ctgctgttca acgatcacat cgccactgcg gaggaagcgc
     4021 tcatagatgc ggtcgagcag ctcgcgagcg ccggcatcga cgaactcgtt ctcgacattc
     4081 gctacaacgg cggcggctac ctggcaatcg caagccaggt cgcgtacatg atcgccggcg
     4141 atgcgcgcac cgcggggcaa acgttcgagg agatccagtt caacgacaag cacccctcta
     4201 ccaacccggt aacggggcag ccgctgcagc cgtacccatt cctcgatacg accgccgggt
     4261 tctcttcgct gccctcagga acgccgctcc cggcgctgga cctcgagcgc gtggtggtta
     4321 tcacaggccc taatacctgc tccgccagcg agtcgatcat gaacagcctg cgcggcgtcg
     4381 acgtcgaagt catccagatc ggctccacga cctgcggcaa accgtacggc ttctacccta
     4441 ccgataattg cggcacgacg tatttcacga tccagttccg cggcgtcaac gataaaggat
     4501 tcggcgatta cacggatgga ttctcgccat tcaacaccgc attggtcgaa ggcgagctgg
     4561 tgccgggctg ctccgtagcc gacgatttcg aaaacgcgtt gggcgacccg ctggagaaac
     4621 gattccagtc ggcgctcgcc tacctggaga ccggaagctg ccctacgccc tccggtgtga
     4681 ccgccccggg actgagcaag acaaaggcac tgctctccgc gaccgacggt ctcatgccca
     4741 aatcgccatg gcgcgaaaat cgcattctca ggcgatagcg catgaattcc gcaaaggtat
     4801 ggttgatcct gctattcacg atgtcgctcg cggggtgcaa tttgaacggc gtgcatgatg
     4861 aacgtcccgc cctcatcgtc gcaccgaccg cggaaagccg cgccgagctg gagcgcgcgg
     4921 tgagcgaaat gttgcacggt gcggatgtca tgctcgccga cgacgcgctg acggcgtcga
     4981 gcatactggt ggtggagagg aacaggattc gcagcatgga gaaccggccg ctgtccggac
     5041 gcgacctcgg ccgccccgag cggttccggc tggtcacaac gggcgatcgc tgcgttttga
     5101 tccacgaaaa cgacgacgcg cgacgcgaac tgctcgagac cgaatgcgtt cctgaataga
     5161 aaggctgtag gagccccgcc catgcgggcg agttcgccaa c
//
LOCUS       DASUOM010000356         7414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1476167,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000356 DASUOM010000000
VERSION     DASUOM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476167"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5042)
                     /locus_tag="VFY27_07485"
     CDS             complement(<1..5042)
                     /locus_tag="VFY27_07485"
                     /EC_number="2.4.1.25"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:NF011077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5765394.1"
                     /translation="MNNSPLDRLCALYGVANEYHDITGKLHHVPDETRKLLLRAMGLE
                     LESDEDVVRMLIAAEEERWCRPLEPVAIGRAGDANLALVVALPEAEMHRPVHWHLTEE
                     SGRTANGSFVPAECEVGERRDIDGKPWLRITLAIPAPGAEGYHRIRLYEDGSPRQIAE
                     SLVVWAPARCYRPEPLAEGRRLWGLTAQLYGLRSQRNWGIGDFTDLLRLVEFAAGNGA
                     AFVGLNPLHSPLRHDDEHASPYDPSSRRFLNALYLDVESIPDFEESEEARKLVAYEDF
                     QAELRSLRAAELVDYQAVSALKHEVLRLLFENFRRRHLQGGSGRARAFEEFQRDRGPL
                     LQQFASFQVLYEHWYGKDPQAWGWPAWPEAFQDCNSGAVAKFLETRGDEIAFFMYLQW
                     NADLQLSAAARRGDELGMPIGIYADLALSSKNGGADTWIDRDLFIRGAHIGAPPDPFN
                     QAGQDWGLPPWNPARLRRAGFRPFIDTLRANMRIGGALRIDHVMALMRCFLLPAGSNA
                     AGGTYVHYPFDEMRAVLALESQRQRCLVIGEDLGTVPDEVRRGLSESGVLGYRVLYFE
                     KNEQGSYKQPGDFDAEVLTVISTHDLPTLLGFWQGADIEWRTANDLFPSAENREQQLV
                     ARAEDRARLMVALEQQRLVPGEPERSELQVGADMTGELSSAFHRYLARSESLLVGVQV
                     EDLLGERGQANLPGTTGEHPNWRRRLSLALESWTDEPQVAQILSAIREERPAAGAETH
                     LKAVRPQRRATIPRATYRVQLHKDFGFADLERLVPYLAMLGISHVYCSPYMKARAGSM
                     HGYDVVDHNSFNPEIGDRAGFDRFCGALSAHGMGHILDMVPNHMAVTGRDHEWWLDVL
                     ENGPASRFADFFDIDWQPLKEELRGKVLLPVLGDHYGSVLDEGKLELLFDDASGSFAI
                     GYFEHCFPIDPREYPQILQPDIDLLRNRLQDDDTPLLLFESLVTAFGNLPGRDCREQD
                     VRRERARDQRLHRQRLAELAASAPDIRRYVDECLRMFNGDEQYPADVGRLHRLLESQA
                     YRLAYWRVASHEINYRRFFDINELASLRMENPEVFEATHGLVLELIEAGKLQGLRIDH
                     PDGLYDPRGYFRTLQQRVGVLPVDFESHSKDLPLYILAEKILAAQETLRSDWPIHGTT
                     GYEFGNLVSRLLVRPDGLADLDGAYHGFIGKSAAYEEILHRSKTLIMQTSLASELNVL
                     AAELDRLAEQDPHTRDFGLGGLRSALLETVACFSVYRTYCVDGEVSDEDKAIIDHAID
                     SAKRRSEAADTSVFEFLRDVLLNRSAIGRSEELKSRVARFGMRLQQYTSPVAAKGMED
                     TAFYRYARLLSVNEVGGTPEQPYVSIEEFHGANLERKARWPHSMLASSTHDTKRSEDV
                     RARLHVLSEMAAEWKQHVELWSRVNEPFKGHSNEGSLVDRETEYFLYQTLLGAWPLED
                     DVSRDETGHEHFVGRMASYMIKAAKEAKRHTSWLNPDLDYEETLQRFVGALLDPASNQ
                     SFMEDFLPFQRRVARLGLYNALVAVLLKLTCPGVPDLYQGNELWRFDLVDPDNRRPVD
                     FDRRKRLLDDIRDRFERGDSASMLAEFLDTLEDGRAKMFVTWRALALRSEHAALFEQG
                     SYIPLAVSGDCAAHVFAFARSWQGKTVVVAVLRWIAQILGSGGETLDVSRLCGTSVEL
                     PAGRHRYIDAFTG"
     gene            complement(5046..6887)
                     /gene="treZ"
                     /locus_tag="VFY27_07490"
     CDS             complement(5046..6887)
                     /gene="treZ"
                     /locus_tag="VFY27_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX5765395.1"
                     /translation="MSRGQVHPMPFGAELSKDGSARFRLWAPDAERVDLCTEDDAGPR
                     IVNMGAGRNGWFELETADAPAGTLYRYRINGELLVPDPASRFQPQGVNGPSEVIDASA
                     YDWQRAWQGRPWHEAVVYELHVGTFTPGGTFESAQERLGHLSELGVTAVEVMPVATFA
                     GNRNWGYDGVLPYAPHAAYGRPDQFRTFVDAAHEHGIMVLLDVVYNHFGPEGNHLHSY
                     ASPFFTARHQTPWGDAINYDGELSRNVRDYFLHNALYWLEEYRLDGLRLDAVHAIIDD
                     SNTHILDELADAVLSGPGGQRQIHLVLENYDNEASFLKPAAVRERPRYAAQWNDDFHH
                     ACHVLITGECDGYYIDYADKPVAHLARCLCEGFAYQGEESRFNGVRRGEPSSQLPLTS
                     FISFLQNHDQVGNRAFGERLVELSTTPALRAATSVMLLSPGVPGLFMGEEFGCRQPFL
                     FFADFEGDLAEAVRQGRRKEFAAFAQFGDGGSAGAAIPDPIAVETFERSVLQWDKLAE
                     PEHAEWVEFYRQLLDLRRRFVVPHLPPSAATAPQFLGERGLRITWKLEGSVELTLLAN
                     LGDETLQLDAGQRPPGRLLHAQPGTAGTIAPADELPPWTAIWHINDE"
     gene            complement(6892..>7414)
                     /locus_tag="VFY27_07495"
     CDS             complement(6892..>7414)
                     /locus_tag="VFY27_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEX5765396.1"
                     /translation="NNVYCQDNEISWMNWEFGNEDRSLLAFVQQLIFLRKAHPVFRRR
                     RFFQGQPIKGASVKDILWLDPAATEMTEEEWVSSHAKTLGVLLSGEGIEELDARGERI
                     LDDTFLVLMNAHHEPMPFTLPALASGTRWLAVFDTARDGGVKSQGRYQASGEYPLEGR
                     SVVVLSQPNVNRP"
BASE COUNT         1364 a         2399 c         2213 g         1438 t
ORIGIN      
        1 ccggtaaagg cgtcgatgta cctgtgccgg ccggcgggca gttcgaccga cgttccgcac
       61 agccggctca cgtccagcgt ttcgccgccg gagccgagga tttgtgcaat ccatctcagc
      121 acggccacca ctacggtctt gccctgccag ctcctggcaa acgcgaagac atgcgcggcg
      181 cagtcgccgc tgacagcaag tggaatgtaa cttccttgct cgaacagcgc ggcgtgttcg
      241 cttcgcaggg ccagcgcacg ccaggtcacg aacatttttg cgcggccatc ttccagcgtg
      301 tcgagaaatt ctgcaagcat gctcgcggag tcaccacgct cgaacctgtc acggatgtca
      361 tccagcagcc gcttccggcg gtcgaagtcc accggccggc gattgtccgg atcgacaaga
      421 tcgaagcgcc agagctcgtt tccctggtac aggtccggga ctcccggaca ggtcagcttc
      481 aacagcaccg cgacaagcgc gttgtatagc ccgaggcggg ccacgcgcct ctggaacggc
      541 aggaaatctt ccatgaacga ctggttcgac gcggggtcga gcagcgcacc aacgaatctc
      601 tgcaaagttt cttcgtaatc caggtcagga ttcagccagg atgtgtgccg cttcgcctcc
      661 ttggcagcct tgatcatgta cgaggccatt cgcccgacaa aatgttcatg tcccgtttcg
      721 tcccggctga catcgtcttc caggggccat gcgccaagga gggtctggta gagaaagtat
      781 tcggtctcac gatccaccag gctcccttcg ttgctgtgtc ccttaaaggg ttcatttact
      841 ctcgaccata gttcgacgtg ctgcttccac tccgccgcca tctccgataa cacgtgaagc
      901 cgcgcccgta cgtcctcgga acgcttggta tcgtgggtgg atgatgcgag catcgagtgc
      961 ggccaacgcg ccttgcgttc gaggttcgcg ccatgaaact cctcgattga cacgtatggt
     1021 tgttccgggg tacccccgac ctcgttcacg gaaagcaaac gtgcatagcg gtagaacgcg
     1081 gtgtcttcca ttccttttgc tgcgacaggg ctcgtgtact gctgcagccg catgccaaac
     1141 ctggcaaccc ggctcttcag ctcctcgctt cggccgattg cgctgcgatt caacaggacg
     1201 tcgcgcagga attcgaagac gctcgtgtcc gcggcctcgc tgcgtctttt cgcagaatct
     1261 atcgcgtgat ctatgatcgc cttgtcttcg tccgatactt cgccgtccac acaataggtt
     1321 cgataaacgg aaaaacaggc caccgtctcg aggagcgccg atcgcaaacc gcccaggccg
     1381 aagtcccgtg tatgcgggtc ctgctccgcc aggcggtcca gctctgcggc cagcacgttc
     1441 agttcactgg ccagcgaggt ttgcatgatg agcgtcttcg agcggtgcag gatttcctcg
     1501 taggcggcgc tcttgccaat gaagccgtgg tacgcgccat ccaggtccgc gaggccgtcc
     1561 gggcggacca gcaagcggct tacgagattc ccgaattcgt agcctgtggt tccgtggatg
     1621 ggccagtcgc tgcgcagcgt ttcctgggcg gccagtatct tttccgccag tatgtacagc
     1681 ggcaggtcct tggagtgact ttcgaaatcc accggcagca caccgacacg ctgctgcagc
     1741 gtgcggaagt aaccacgcgg atcgtacaac ccgtcagggt gatcgatgcg gagaccctgc
     1801 agcttgccgg cctcgatcaa ctccaggacg agaccatgag tcgcctcgaa cacctccgga
     1861 ttctccatac gcagagacgc caactcgttg atgtcgaaga agcggcggta attgatctcg
     1921 tgcgacgcca cccgccagta cgcaaggcgg tacgcctggg actcgagcaa ccggtgcagg
     1981 cgccccacgt cggcggggta ctgctcatct ccgttgaaca tacgaaggca ttcatcgacg
     2041 tagcgccgta tgtccggtgc actcgctgcc agctccgcca gccgctgccg gtggagcctc
     2101 tggtcgcggg cgcgttcccg gcggacgtcc tgctccctgc agtcgcgccc gggcaggttg
     2161 ccgaacgccg ttacgaggct ctcgaacagc agcaggggtg tgtcgtcatc ctgcaggcgg
     2221 ttgcgtaaca ggtcgatatc gggctgcaga atttgcggat actcccgggg gtcgatcgga
     2281 aagcaatgtt caaaataccc gatggcaaag cttccgctcg cgtcatcgaa aagcagctct
     2341 agctttccct cgtccagcac gctgccgtag tgatcgccga ggaccggcag cagcaccttc
     2401 ccgcgcagct cctccttcag cggctgccag tcgatgtcga agaaatcggc gaagcgggaa
     2461 gccggaccgt tctccaggac gtccagccac cactcatggt cgcgtccggt taccgccatg
     2521 tggttcggca ccatgtccag tatgtgaccc atgccatgcg cggacagcgc accacagaag
     2581 cggtcaaagc cggcgcggtc gcctatttcc ggattgaacg aattgtgatc gacgacgtcg
     2641 tagccgtgca tgctgccggc gcgcgccttc atgtatggcg aacagtagac gtgactgatg
     2701 ccgagcatcg cgaggtaagg cacgagccgc tcgaggtccg caaaaccgaa gtccttgtgc
     2761 agctgcacgc gataggtggc gcgcggtatc gtcgctcgcc gctgcggccg caccgccttc
     2821 aagtgagtct ccgctccggc tgcgggacgt tcctcacgaa tggccgagag tatttgcgct
     2881 acctgtggtt cgtcggtcca gctttcgagc gccagtgaca aacgccgacg ccagttcggg
     2941 tgctccccgg ttgtgccggg caggttcgcc tgcccgcgct ctccaagcag gtcctcgacc
     3001 tggacaccga ccagcaaact ctcgctcctc gccaggtacc ggtggaaggc ggagctcagc
     3061 tccccggtca tgtccgcgcc gacctgcagc tcgctgcgtt cgggttcccc cgggacaagt
     3121 cgctgctgct ccagcgcgac catcagccgt gcgcgatctt ccgcgcgggc gaccagttgc
     3181 tgttcccgat tctcggcgct ggggaaaagg tcgttcgcgg tacgccactc gatgtcggcg
     3241 ccctgccaga agccaaggag cgtgggtaag tcgtgcgtgc ttatgaccgt caggacctcc
     3301 gcatcgaaat cgcccggctg cttgtaactg ccctgctcgt ttttttcgaa gtacagcact
     3361 cgataaccca ggacgccgct ttcgctcaga ccgcgccgaa cctcgtccgg gactgttccc
     3421 aggtcctctc cgatgaccag gcagcgctgc cgctggctct cgagggccag gaccgcgcgc
     3481 atctcgtcga acgggtaatg aacgtaggtt cccccggcgg cgttgcttcc ggcaggcagg
     3541 agaaaacagc gcatgagcgc catgacgtgg tcgatgcgta acgcaccgcc gatgcgcatg
     3601 ttcgcgcgca gggtgtcgat gaacggacga aaccctgctc tgcgcagtcg tgccggattc
     3661 cacggcggca ggccccagtc ctgccccgcc tggttgaatg gatcgggcgg cgcgccgata
     3721 tgcgcgcctc ttatgaacaa atcacgatcg atccaggtat cggctccgcc gttcttggaa
     3781 ctcagcgcca ggtccgcata aatgccgatt ggcataccga gttcatcgcc gcggcgggcc
     3841 gctgccgaca actggaggtc ggcgttccat tgcaggtaca tgaagaaggc gatttcgtca
     3901 ccgcgggtct cgaggaactt cgcaactgcc ccggagttgc agtcctggaa tgcctccggc
     3961 cacgccggcc atccccatgc ctgcggatcc ttcccgtacc agtgctcata aagcacctgg
     4021 aaggaggcaa actgctgcag caatggccct cggtcgcgct ggaattcttc gaaggcccgg
     4081 gctcgtccgc tccccccttg caggtggcgg cggcggaagt tctcgaacag caggcgcagc
     4141 acctcgtgct tcagcgcact tactgcctgg tagtcgacca gttccgccgc gcgcaggctg
     4201 cgcagttccg cctggaaatc ctcgtaggca accagctttc tcgcctcctc ggactcttcg
     4261 aagtccggga tggattcgac atcgagataa agggcgttca ggaacctgcg gctggacggg
     4321 tcgtatgggc tcgcgtgctc gtcgtcgtgc ctgagagggc tgtgcagtgg attcaggccg
     4381 acgaacgcgg cgccgttacc ggcggcgaac tcgacaagcc gcaacaggtc ggtgaaatct
     4441 ccgatgcccc agttgcgctg cgaacgcagg ccgtacagct gcgcggtcaa tccccacagc
     4501 cgcctgcctt ccgcaagcgg ctccggccga taacagcgtg ccggcgccca cacgaccagg
     4561 gattcggcaa tctgtctcgg cgaaccatcc tcgtagaggc gtatccggtg ataaccctcc
     4621 gccccaggcg cgggaattgc cagcgtgatg cgtaaccacg gcttaccatc gatatctcgc
     4681 cgctccccga cctcgcattc cgccgggacg aacgaaccgt tcgcggtccg tcccgactct
     4741 tcggtcaagt gccagtggac cgggcggtgc atttccgctt ctggcagagc aacgacaagc
     4801 gccaggttcg cgtcccccgc ccgcccaatg gcgaccggct ccagcggtct gcaccatcgc
     4861 tcctcctccg cagcgatgag catgcgaacg acgtcttcgt cgctctccag ttcgagaccc
     4921 atcgcacgca gcaacaactt gcgcgtctcg tccggtacgt gatgcagctt gcctgttatg
     4981 tcgtggtatt cgtttgcgac accgtacagc gcgcaaagcc ggtcgagagg agagttgttc
     5041 acttattact cgtcgttgat gtgccagatc gcagtccagg gggggagttc gtccgccggg
     5101 gcaatagtgc ccgcggtccc aggctgcgcg tgcaggaggc gtcccggcgg acgctgcccc
     5161 gcatccagct gcagcgtctc gtcacccagg ttggcgagca gcgtgagttc gacgctaccc
     5221 tcgagtttcc aggtgattcg tagccccctt tcccccagga actgcggcgc tgttgctgcc
     5281 gacggaggaa ggtgaggcac tacgaaacgc cgccgcagat cgagtagctg ccgataaaac
     5341 tcaacccact cggcgtgctc cggttccgca agcttgtccc actgcagtac gctgcgttcg
     5401 aaggtctcga cagcgatggg atcaggaatc gcagcaccgg cacttccgcc gtcgccgaat
     5461 tgcgcaaagg cggcgaactc ctttcttcga ccctgacgga ccgcctcggc aagatcgccc
     5521 tcgaagtccg caaagaacag gaacggttgc cggcacccga actcctcacc catgaaaagt
     5581 ccgggcacgc cggggctcaa aagcatgacg ctcgtggctg cccgaagtgc gggtgtcgtt
     5641 gaaagctcga ccagccgctc gccgaacgcg cgattgccga cctggtcgtg gttctgcagg
     5701 aacgaaatga acgaagtcag tggcaattga ctgctgggct cgccgcgacg caccccattg
     5761 aacctcgact cctccccctg gtatgcgaaa ccttcgcaaa ggcaacgcgc aagatgcgcg
     5821 acgggcttgt ctgcgtagtc gatgtagtag ccgtcacact cgccagtgat gagaacatgg
     5881 catgcatgat gaaagtcgtc gttccattgc gcagcgtatc gcggccgctc gcgcacagcg
     5941 gcaggcttga gaaagctggc ttcgttgtcg tagttctcga gcaccaggtg tatctgtcgt
     6001 tgtccgccgg ggccgctcag tacggcgtct gccagttcat ccagtatatg cgtgttggag
     6061 tcgtcgatga tggcgtgcac ggcatcgagg cgcagcccgt ccagccggta ttcctcgagc
     6121 cagtagagcg cgttgtgcag gaaatagtcg cgcacgttgc ggctcaattc gccatcgtag
     6181 tttatggcgt caccccacgg cgtctggtgc ctggcggtaa agaacgggct cgcgtaggaa
     6241 tgcaggtgat tcccctcggg accgaagtgg ttgtatacga cgtccagcag taccattatt
     6301 ccgtgctcat gcgctgcatc cacgaacgtc ctgaactggt ccgggcgtcc atacgcagcg
     6361 tgcggcgcgt atggcagtac gccgtcataa ccccagttcc ggtttcccgc gaacgtcgcc
     6421 accggcatta cttcgacggc ggtaacgccg agttccgaaa ggtgcccgag gcgttcttgc
     6481 gccgattcga aggtcccgcc gggggtgaac gtaccgacgt gcagctcgta cacgaccgcc
     6541 tcgtgccacg gtcgtccctg ccacgcccgc tgccagtcgt atgcagacgc atcgataact
     6601 tcgcttggcc cgttgacgcc ctggggctgg aaccgtgaag cggggtccgg gacgagaagt
     6661 tccccgttta tgcgatacct gtaaagagtg cccgcagggg catcggcagt ctcgagttca
     6721 aaccagccgt tgcggccggc gcccatgttc actatccgcg gaccggcgtc atcctcggtg
     6781 caaaggtcta cgcgttcggc atcgggtgcc cagagacgga acctggcaga gccatccttg
     6841 ctcagctccg cgccaaaggg catcgggtga acctggccgc ggctcatcgc ctcatggccg
     6901 gtttacgttg ggttggctca agaccacaac agaccgtcct tccagaggat actcgccgga
     6961 ggcctggtag cggccctgtg acttgacgcc accgtcgcgc gcggtgtcga aaacggcaag
     7021 ccatcgtgtc ccgctcgcca gggcaggcag ggtgaagggc atcggctcgt gatgcgcgtt
     7081 catcaggacg aggaacgtat cgtccagtat ccgctccccg cgcgcatcga gctcctcgat
     7141 accttcgccc gacaaaagta ctcccagcgt tttcgcatga gagctgaccc actcttcctc
     7201 ggtcatctcc gttgcagcag gatcaagcca tagtatgtct ttcacactcg cgcccttgat
     7261 cggctggccc tgaaaaaaac gccgcctgcg aaacaccggg tgagctttgc gcaggaagat
     7321 caattgctgc acgaaggcaa gcaaagacct gtcctcgttc ccgaactccc agttcatcca
     7381 gctaatttcg ttatcctggc aatagacatt gttg
//
LOCUS       DASUOM010000357         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_681064,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000357 DASUOM010000000
VERSION     DASUOM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681064"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..1947)
                     /locus_tag="VFY27_07500"
     CDS             complement(463..1947)
                     /locus_tag="VFY27_07500"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5765397.1"
                     /translation="MPQGRRFAPLNAVGLMLIGVAYFVVAYLGLQLASINPSATPIWP
                     ATGLAIAAILAWDYRVAPAIFIAAFLVNYVTAGSAMTAAAIGLGNTLEGVATAYLVKL
                     WAGGLHVFESPAGVGKFALISAATTTISATTGVGSLAIAGYAETGKVGAIWLTWWLGD
                     LAGAVVITPMVLLWIVPQATRPSFRETALVYGAATAIGIVAFSPLLPQTGLRDPLGFL
                     AVGPLLWAALRCGPRDTATVAAILATFAVWGTLMEGGPFARANLNQSFLLLLMFLIST
                     AIPSLALSADISGRQRAHEQQQLLLRELSHRVGNSLAVLNSVFRRSARHASSIADLEE
                     AFQGRLMSLAATHRLLGESNWESASMLDLVRAAVDPYCPSVHRGCTFEGGDVRIPGSL
                     VTSITMVLHELATNAARHGALKSPLGQLKVSWRCESDCEGCAQLHVNWDELGAGRQQN
                     VAGNAAGYGTALIDSTIATLGGRVTRSSGRQGYSVRLSVPLGWT"
     gene            2216..3214
                     /locus_tag="VFY27_07505"
     CDS             2216..3214
                     /locus_tag="VFY27_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5765398.1"
                     /translation="MSLSDVIHSLRERPLLALAVALFIFLAAFGVRYSFSSDLYAVPF
                     ITLLPAILISALIGGTWIGVIVAILSALVAWFWFLPPSATTALRWPDGYLTWALFALT
                     CFIQLYVIHALNQTVEALSRARDRSTALFLELQHRVANNLQFVASMLRMKRRVLELDP
                     TAGDRLFESAQSRLNHMARIHRRLYSPESLELPVEEFFQGLCTDILTARGVQNVNCQA
                     NAAQVRLDLQQLVALGLLINEFVTNSAKYAFTGLEAGNISISLEQSDADHLTLTMEDD
                     GRGLPVGLDLTNGTSLGLRIMNSLATQLGGELSWIEVSDGAAAKLVFPLRSDAAQT"
     gene            complement(3290..4423)
                     /locus_tag="VFY27_07510"
     CDS             complement(3290..4423)
                     /locus_tag="VFY27_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5765399.1"
                     /translation="MITSKTAIAQIEQAQWWGFSGVAKHNPFAAIAHGHSLAEAIVDA
                     IREPLLVLDPDLRVIAASRSFYRTFAVMPRKTEGRLLFELGDGQWNIPGLRKLLVDII
                     PKRRTVEAYEVEHEFPSIGRRVMLLNARRVFDEDGTASAILLAIEDVTRRREAEREKD
                     DLLQQKEILLQEMQHRVANSLQIIASILLLKARTVQSEETRLHLQDAHQRVMSVATVQ
                     QQLHASGLNERIEIGPYLTKLCDSLAASMIGERRPLSFKVLASSGGAVSSEAVSLGLI
                     VTELVINALKHGFQGQEQGEILVSYDAQPSGWRLSVSDNGSGLKDAGGEPPHTGLGTS
                     IVEALAHQLEATVEKSSGPEGTRVTIIASSGRCAALDQVDRAR"
BASE COUNT          914 a         1330 c         1273 g          936 t
ORIGIN      
        1 gctatggctg caacgatgtt tgcggtagtg ccataaacaa tcgcaaattc ccagaatgca
       61 cgacccaatg ggatctcacc tcgccagagc gctgccaagg tcttaagcat gctgaaagcc
      121 cactgctaag ggcgccgggt ccgaacaaat tagccccgtc ccgtgcgaga cactaaaggg
      181 gaaaatgagc tgcaccgttg cctcttccga ggcatccagc cagggtagtt cgaccgtcgg
      241 cttaggattg cgtgccgcca tgttagaatc ttgccgtctc gcgccagggc aacctttggg
      301 tttcgggtga aacaacgtct agtgttggca ccgagcagac gtgccagctg gcgagcctta
      361 cgtcttctga ccctatgcag accaaggcgc acagctgcgg gcgtgtcgtt ggtgccacca
      421 cggtcattcc ttgaacagga ggaaagggct cgtttgacga gtctaagtcc agccaagcgg
      481 aactgacagt ctgacagagt agccctgccg tcctgaggac ctagtgacgc gccctcccaa
      541 agtcgcgatg gtagagtcga tcaaggccgt tccgtaacct gcagcgttcc cggcaacgtt
      601 ctgctgtcgc cctgcaccga gttcatccca atttacatgc agctgcgcgc atccttcgca
      661 gtcagattcg caacgccagg aaactttgag ctggccaagg ggcgacttta gtgcgccgtg
      721 tctggccgcg ttcgttgcaa gctcatgaag caccatggtg atagaagtca ccagcgatcc
      781 cggaatccgc acatcaccac cttcgaacgt gcacccgcga tgcacagaag gacagtatgg
      841 atccacggcc gctctgacca aatccagcat tgaggccgat tcccagttgc tctctccaag
      901 tagccgatgg gtggccgcca ggctcatcag gcggccctga aaggcttctt cgaggtcagc
      961 gatgctactc gcgtgacgcg ctgaacgccg gaagaccgag ttgagtacgg ccaagctgtt
     1021 accaacccgg tggctcagct cgcgcagtaa gagctgctgc tgctcgtgtg cgcgttgacg
     1081 accgctgatg tcggcactca gggcaagcga gggaatcgct gtgctaatca agaacatcag
     1141 aagcaacagg aaggattggt tcaggttcgc gcgcgcgaag ggaccacctt ccatgagcgt
     1201 gccccacacg gcgaatgtcg ccaaaatggc cgccaccgtc gccgtgtcgc gcggaccgca
     1261 ccgcaatgcg gcccagagca gcggcccgac cgcaaggaaa cccagcggat ccctcaaccc
     1321 tgtctgcggt aggagtgggc tgaaggcaac tatgccgatc gcggtcgccg cgccatagac
     1381 caaggcggtt tccctgaaac taggccgcgt tgcctgaggt acgatccaca ggagaaccat
     1441 cggcgttatg actacggcgc cggcaagatc gccaagccac caagtgagcc agattgcacc
     1501 gactttaccg gtctcggcat atccggcgat cgccaagctg ccgacaccgg tcgttgcact
     1561 aatggtggtc gtggcggcgc tgatgagcgc gaactttccc acgcccgctg gagactcaaa
     1621 gacgtgtagg ccacccgccc aaagctttac tagataagcg gtcgcgaccc cttcgagcgt
     1681 attgcccagc ccgattgcgg ctgcggtcat ggcggaccca gccgtgacgt aattgacgag
     1741 aaaggctgcg ataaaaatgg caggcgccac gcggtaatcc caagccaaga tcgccgcgat
     1801 ggcaagcccg gtagcgggcc agatcggcgt tgcactcgga ttgatcgagg cgagctgcag
     1861 gccgagatag gccacgacga aataggccac cccaataagc atcagcccta ccgcgttcag
     1921 cggcgcgaag cgtcgacctt ggggcatcgc cgtagccttt ggaggtggcg aaataactga
     1981 gacttctcgc cacgacggga tgagacaacg gcttttcagg cgtctcggtt cctgtcgcag
     2041 tcacgacgca cgtgccacgt tgctgtcggg ggtctagaat tgccgctccc tgtcggagat
     2101 ttcgcatcgg caggtagagt cttgcaggat gtgatttggc cgataacact agcttttcta
     2161 acaggactgc tcgctgtctg ggagcactcc ttgagctcgg ccgaggcgtt acgtcatgag
     2221 tctcagcgat gtcattcaca gcctgcgcga gcgtccactg cttgcgctcg cggttgcact
     2281 gttcatattc ctggccgcgt ttggtgttcg ctatagcttt tcaagcgacc tttatgccgt
     2341 cccgttcatc acgctcttgc ccgcaatttt gatctcagcg ctgatcgggg gaacttggat
     2401 cggcgtcatt gtggcgatac tcagcgcgct ggttgcctgg ttttggttcc tcccgccctc
     2461 cgcaaccact gcactacgct ggccagacgg ttatctcacg tgggcccttt ttgcgctcac
     2521 ctgcttcatt caactctatg tcattcatgc actcaatcag acggtagaag cattgtcgcg
     2581 cgcgcgtgac cgttccacag cgttgtttct ggagctgcag catcgcgtgg cgaacaatct
     2641 tcaatttgtc gcttcgatgc ttcgcatgaa gcggcgcgta ttggaattgg atccgactgc
     2701 cggagaccga ttgttcgaaa gtgctcaaag ccgtctcaat cacatggcgc gcatccatcg
     2761 gcgactctac agtcccgagt ctctcgaatt gccggttgaa gagttttttc agggactttg
     2821 cacagacatc ctcaccgcta gaggcgtgca gaatgtgaat tgtcaggcga atgctgctca
     2881 ggtcagactt gaccttcaac aactggttgc ccttggactg ctcataaacg agttcgtcac
     2941 gaattctgca aagtatgcct tcaccgggct cgaggcagga aacattagca tttccctcga
     3001 gcagagcgat gccgatcatc tgacattgac catggaggat gatggtcgtg gccttcccgt
     3061 aggactggat ttgactaacg gaacgagcct aggcctaagg attatgaata gtctcgcaac
     3121 tcagcttgga ggtgaattgt cttggatcga ggtgtcggac ggtgcagcgg cgaagcttgt
     3181 gtttccgctg aggtccgatg cggcccaaac ctgatgtccg gttatggcac caaacagaca
     3241 cgctaactgg ctagcctcgc gtcagctttt gacccggagc ggacgtggct tatcgtgcgc
     3301 gatccacctg atcgagtgcg gcgcatcgac ctgaactggc tataattgtc accctcgtgc
     3361 cctccggtcc gctcgatttc tcgaccgtgg cttcgagttg atgcgccaat gcttcgacga
     3421 tgctcgtgcc gagacccgta tgcgggggct ctccgcccgc atcctttagc cccgatccat
     3481 tgtcggaaac cgagaggcgc cagccagacg gttgcgcgtc gtaactgacc aggatctcgc
     3541 cctgctcttg accctggaag ccatgcttta gagcgttaat caccaactcc gtcacgatca
     3601 gcccgaggct cacagcttcg ctggataccg ccccgccaga gctcgctagc accttgaacg
     3661 aaagcggccg gcgttcgccg atcatcgagg ctgcaagact gtcgcacagc ttcgtgagat
     3721 agggcccgat ctcgatcctc tcatttagcc cagaggcgtg cagttgttgc tgcaccgtcg
     3781 ccaccgacat gactcgctgg tgagcatcct gcaagtgcag gcgagtctcc tccgactgca
     3841 cggtcctcgc cttgagcaga agaatactgg cgatgatctg aagactgttg gcaacgcgat
     3901 gctgcatctc ctgaagcagg atttccttct gttgcagcag gtcgtctttt tcgcgctcag
     3961 cctcgcggcg gcgagtcacg tcctctatcg cgagtaggat ggccgaggcc gtaccatctt
     4021 cgtcaaacac gcgtcgggca ttgaggagca tcacgcgtcg cccgatcgag gggaactcgt
     4081 gctcgacttc gtacgcctcg acggtgcgtc gtttcggaat gatgtctacg aggagcttac
     4141 gaagccctgg aatattccat tgaccatccc ccagctcgaa gagtaggcgc ccttccgttt
     4201 tgcgcggcat cacggcaaaa gtccggtaga acgaacggct cgcggcgatc acgcgcaagt
     4261 ccgggtcgag caccagaagg ggctcgcgta tggcgtcgac gatggcctca gccaggctgt
     4321 gcccatgcgc gatagcagcg aatggattgt gcttggccac gccagaaaac ccccaccact
     4381 gtgcctgctc gatctgcgct atagctgtct tggatgttat caaactttct ccctgaaaag
     4441 ggccctaaga gaa
//
LOCUS       DASUOM010000358         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_84239,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000358 DASUOM010000000
VERSION     DASUOM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84239"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFY27_07515"
     CDS             <1..558
                     /locus_tag="VFY27_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5765400.1"
                     /translation="LIGMVTRLTPQKGIDLVQKVLPDLLQQANFSLVVLGSGERDYER
                     FFSWLQESFAGRVSFHRGYNNKLAHRIEAGADIFLMPSLYEPCGLNQMYSLRYGTVPI
                     VRHTGGLADSVQLYDPLTRKGDGIVFRDYDEPALRWAIETALELYTDRPVWRRMMQNG
                     MSKDFSWDTQGKQYVDLFRQLSGRY"
     gene            592..1812
                     /locus_tag="VFY27_07520"
     CDS             592..1812
                     /locus_tag="VFY27_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5765401.1"
                     /translation="MNVIFIEPSFPYNQREFVRALNATGANVIGIGERPAESLGAEMR
                     GWLGDYLQVQSVVHEPSLLQAVKAVQGKLWVDRLEATVEAHIMAAANVRRLTGIPGTS
                     PETAWLCRDKPAMKEALRRAGVPCAQSTRAQSPRDARDFAESVGYPLIIKPPAGAGAS
                     GTWRVDGREALERIIQESGLAAGNAVAIEEFVEGHEGFLDTLAINGEVAHEFITHYYP
                     NVLEAMRERWISPQMVTTNRIDSPGYAEVRKMARDVIRILNIGTSATHMEWFAGPKGL
                     RFSEVGCRPPGVGQWDVYNAANDFDLYFEWACAIVHGRAHKKPSRRYSAGMIALRPER
                     DGYITGYSGVDEIGRRYGDCIVAAHLPAPGTPTQPIEEGYMANAWMRLRHPDYDQLRN
                     IMNEIGETVRVHAG"
     gene            complement(1938..3614)
                     /locus_tag="VFY27_07525"
     CDS             complement(1938..3614)
                     /locus_tag="VFY27_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX5765402.1"
                     /translation="MTADKLRLVLCWHMHQPDYRDVLNSSWRRPWTWLHGIKDYADMA
                     AHLETVPGARAVVNFSTILLEQISAYAESIRQWENTGSTIGDPLLDALGGSRPYRSLV
                     DADKLELTAACLHAHKMHMIKAFPAYSALAVRAKLAIEQGQPLDETRFRDLLVWYHLA
                     WTGESLRRGFPMLRGMLHKAEGFSEEDGRQLLALIGAVLSQLIQRYRKLCASGKIELS
                     LTPHAHPILPLLLDLASARESRPDVTLPGCRYPGGEARCRWHLERALELHEKYFRIRP
                     RGCWPSEGGVSEAVVPLLREYGFEWTATGTNVLRNSIGQAAEHAHFQKWHRTTASGNI
                     ACFFRDDELSDRIGFQYSQWGAEDAVNDFVARLERIRHDWKGEDVPVVSIIMDGENAW
                     EHFPFNGWFFLQHLYSRLALHPGIELTTFSSLLDDGWDAGELPRLVAGSWVYGDFSVW
                     IGDPEKNRAWELLCAAKEATDRVLDNGRDVDRDAVIEQLGVCEASDWFWWLGGGNAVK
                     DSAAFDSLFRDQLKVLYRLLGETAPAALAEAVDTRASADPAEGAMRRANR"
     gene            complement(3652..4902)
                     /gene="glgC"
                     /locus_tag="VFY27_07530"
     CDS             complement(3652..4902)
                     /gene="glgC"
                     /locus_tag="VFY27_07530"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5765403.1"
                     /translation="MQPRRSGRFVSKLTRSTMAVIMAGGRGSRLGDLTANRAKPAAPF
                     GGKFRIIDFPLSNCVNSGIRQIFVLTQYKAHSLIQHINRGWGYLRGEFGEFIQIVPAQ
                     QQLGEQWYLGTADSVYQNLSIVRESHPEHVLILAGDHIYKMDYGPMIAYHTDKEADIT
                     VGVIEVPLPDAKSFGVMTVDESNRIIDFLEKPENPEPMPGSTDTALASMGIYVFDTDF
                     LCDKLIADSADPSSAHDFGRNIVPGAIELQQRVYAYPFHDVETKAQSYWRDVGTVDGF
                     FKANLELAHVHPELNLYDDQWPIWTYQEQLPPAKFILDEPGRRGAAFNTLVSGGCIVS
                     GAEVRESVLFSNVRVREASKLFKAVVLPRASIGANCRIANAIIDEDCVIPDGTVIGEN
                     AEHDARRFWRTEEGVVLVCPHMLH"
BASE COUNT          951 a         1543 c         1546 g          925 t
ORIGIN      
        1 ctgatcggga tggtgacgcg cctgacgccg cagaagggca ttgacctggt ccagaaggta
       61 cttcccgatt tgctgcagca ggcgaatttt tcgctggtgg tgctcggcag cggggaacgc
      121 gactacgaac ggttcttcag ctggctgcag gagagttttg cgggcagggt ttccttccat
      181 cgcggttata acaacaagct tgcgcaccgg atcgaagcag gtgcagacat cttcctgatg
      241 ccgtcactat acgagccctg cggcctgaac cagatgtaca gcctgcgtta cggcacggtc
      301 ccgatcgtac ggcataccgg cggcctcgct gactccgtgc agctatacga cccgctcacg
      361 agaaaaggcg atggcatcgt gttccgggac tatgacgaac ccgctttgcg ctgggcgatc
      421 gaaaccgcgc tggaactata cacggaccgc ccggtgtggc gccgcatgat gcagaacggc
      481 atgagcaagg acttttcctg ggacacgcag ggaaagcagt acgtggacct gttcaggcaa
      541 ctgagcggaa ggtattgaac ggggccaagg tcccctgtat cgagcccgcc aatgaacgtc
      601 atattcatcg agccttcgtt cccctacaac cagcgcgaat tcgtgcgcgc gctgaacgct
      661 accggtgcaa acgtcatcgg catcggcgag cgcccggcgg aatcccttgg cgcggaaatg
      721 cgcggctggc tgggggacta cctgcaggtg cagtcggtcg ttcacgagcc gtcgcttctg
      781 caggcggtca aggcggtaca gggcaagctg tgggtcgatc ggctcgaggc aacggtcgag
      841 gcgcacatca tggcggctgc gaacgtgcgc cggttgacgg gaatccccgg tacttcaccc
      901 gaaaccgcct ggctgtgccg cgacaagccg gctatgaagg aagcgctcag gcgcgccggc
      961 gttccctgtg cccagtccac gcgggcgcag tcgccgcgag atgcgcgcga cttcgccgaa
     1021 tcggtgggct acccactgat catcaagccg cccgccgggg ccggagcgtc cggaacctgg
     1081 cgcgtcgacg ggagagaggc gctggagcga attatccagg agtcggggct tgcggcgggc
     1141 aacgcagtgg cgatcgagga gttcgtcgaa gggcatgaag gcttcctcga caccctcgcg
     1201 atcaatggcg aggtcgcaca tgaattcatc acccattact acccgaacgt gctggaggcg
     1261 atgcgcgaac gctggatttc tccccaaatg gtgacgacga accgcatcga ctcgccgggc
     1321 tatgccgaag tccggaaaat ggcgcgggac gtgatcagga tactcaacat aggaacctct
     1381 gcgacgcaca tggaatggtt cgccgggccg aaggggctaa ggttctcgga agtcggttgc
     1441 cggccgccgg gcgtcgggca gtgggacgtt tacaatgccg ccaacgattt cgacctgtac
     1501 ttcgaatggg cttgtgcgat cgtccatgga cgcgcgcaca agaagccgtc gcggcggtac
     1561 tccgccggca tgattgcgct gcggccggaa cgcgatggct acatcaccgg gtactccggc
     1621 gttgatgaga tcggccggcg ctatggcgac tgcatcgtcg ccgcgcacct gcccgccccc
     1681 ggcaccccta cgcagccgat cgaggaaggc tacatggcaa acgcgtggat gcgcttgcgc
     1741 cacccggact acgaccagtt gcgaaacatc atgaatgaga ttggagaaac agtacgcgtg
     1801 cacgcggggt gatggcccgg cgtgacgcgt gggtgcttcc ggaccttagt ttgcgcactc
     1861 gcccgcgagc ggcgggctcc tacaacgtcc tgccacttgc cccgtaggag cccgccatgc
     1921 ggacaagtgc gacttgctca gcgattggca cgacgcatgg ccccttctgc ggggtcagcg
     1981 gaagctcgcg tatcgacggc ctccgccagt gccgcaggcg ccgtctctcc gagcagccgg
     2041 tacagtacct tgagctgatc cctgaaaagg ctgtcgaagg cggcgctgtc cttcaccgcg
     2101 ttaccgccgc cgagccacca gaaccagtcg gatgcttcgc agacgccgag ctgctcgata
     2161 acggcgtcgc gatccacgtc ccgtccgttg tcgagcacac gatcggttgc ttccttcgcg
     2221 gcgcacagca attcccaggc ccggttcttc tccggatcgc caatccacac cgagaaatca
     2281 ccatacaccc agcttcccgc gacgaggcgc ggcagctcgc cagcgtccca tccgtcgtcc
     2341 agcagcgaag aaaacgtcgt gagctcgatc ccggggtgca gggccaggcg cgagtacagg
     2401 tgctgcagga agaaccagcc gttaaagggg aaatgctccc acgcattctc gccgtccatg
     2461 atgatgctga cgaccggcac gtcctcgcct ttccagtcgt gccgaatgcg ctcgagccgc
     2521 gctacaaagt cgtttaccgc gtcctcggct ccccactgtg agtactggaa gccgattcga
     2581 tccgaaagtt cgtcatcccg aaaaaaacag gcaatgttcc cgctggccgt cgtccgatgc
     2641 cacttctgga agtgcgcgtg ctccgcagcc tgcccaatcg aattcctgag cacgttggtg
     2701 ccggtggcgg tccattcgaa accgtactcg cgaagcagcg gcactaccgc ttcgctgact
     2761 ccgccctcgg atggccagca gccccgggga cgaatcctga aatacttttc gtgcagttca
     2821 agcgcccgct cgagatgcca gcggcaacgc gcttctccac ccggatagcg gcagccgggc
     2881 aaagtcacat ccggtctcga ttcgcgggcg ctggccaggt cgaggagaag cggcaggatc
     2941 ggatgcgcgt gcggcgtcag cgacagctcg atcttcccgg atgcacacaa cttcctgtat
     3001 cgctggatga gttgcgacag cacagcgccg atcagcgcca ggagctggcg cccgtcctct
     3061 tcgctgaacc cttctgcctt gtgcagcatg ccgcgcagca ttgggaagcc acgccgcagg
     3121 ctctccccgg tccacgcgag gtgataccag accagcaggt cgcggaaccg cgtctcgtcc
     3181 agcggctgtc cttgctcgat tgccagcttc gcccgcaccg ccagcgccga atacgccgga
     3241 aacgccttga tcatgtgcat cttgtgcgca tgaaggcacg ctgcggtcag ttcgagcttg
     3301 tcggcatcga ccaggctccg gtagggtctg ctgcctccga gcgcgtcgag cagcggatca
     3361 ccgattgtgg acccggtgtt ttcccattgc cggatcgatt cggcataggc cgagatctgc
     3421 tcgagaagga tcgtcgagaa attgactacc gctcgtgcac cgggaaccgt ctccaggtgc
     3481 gcggccatgt cagcataatc cttgatgccg tgcagccagg tccatggcct gcgccagctc
     3541 gaattcagca catcccggta gtccggctgg tgcatgtgcc agcacagcac cagccgcagc
     3601 ttgtctgccg tcatgaatgt tccttcctac gaggtgacgc ggacaggaaa atcagtggag
     3661 catgtgcgga cagacgagaa ccacgccctc ttccgtgcgc cagaagcggc gcgcgtcgtg
     3721 ttctgcattc tcaccgatca ccgtgccgtc aggtatgacg caatcttcgt cgatgatcgc
     3781 gttggcgatg cgacagttcg cgccgatact ggcgcgcggc agcaccacgg ctttgaacag
     3841 ctttgacgcc tcgcgaaccc ttacattgga gaacaggacg gactcccgaa cctccgcacc
     3901 ggagacgatg cagcctccgg ataccagggt attgaaagca gcgccgcggc gccccggctc
     3961 gtcgaggatg aactttgccg gcggcagttg ttcctggtac gtccagatgg gccactggtc
     4021 gtcgtacagg ttcagttccg gatgcacgtg cgccagctcc aggttggcct tgaaaaaacc
     4081 gtcgaccgtt ccgacgtcgc gccagtaact ttgcgccttc gtttcgacgt cgtggaaagg
     4141 ataggcgtaa acgcgctgct gcagttcgat ggcgcctgga acgatgttac ggccgaagtc
     4201 gtgcgccgag gatggatcgg cagaatcggc tatcaacttg tcgcagagga aatcggtgtc
     4261 gaaaacatag atgcccatcg atgccagcgc agtatccgtg gaacccggca taggctcggg
     4321 gttttccggc ttctccagga agtcgatgat ccggttgctc tcgtcaacgg tcatgacgcc
     4381 gaacgatttc gcatcgggaa gaggtacctc gatcactccc acggtgatgt cggcttcctt
     4441 gtcggtgtgg taggcaatca tcggaccgta gtccatcttg taaatgtgat cgccggcgag
     4501 aatcaggacg tgctccggat ggctttcgcg cacgatgctc aggttctggt agacggaatc
     4561 cgccgtgccg agataccact gctctcccaa ctgctgctgc gccgggacga tctggatgaa
     4621 ttcgccgaat tcgccccgca ggtagcccca gccgcgattg atgtgctgta tcagcgagtg
     4681 cgccttgtat tgcgtgagca cgaagatctg gcggatcccg gagttgacgc aattggataa
     4741 ggggaaatcg atgatccgga acttgccgcc gaagggggcc gccggctttg cgcgattggc
     4801 ggttaggtca ccgaggcgcg agccgcgtcc gccggccatg atgaccgcca tcgtgctgcg
     4861 ggtcagtttg ctgacaaatc gcccggaccg tcgcggttgc acctgttgct ccctatatag
     4921 cctgtatagc atggccggat tcggccaacg aaacgcagcg gactt
//
LOCUS       DASUOM010000359         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1056809,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000359 DASUOM010000000
VERSION     DASUOM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056809"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFY27_07535"
     CDS             complement(<1..658)
                     /locus_tag="VFY27_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5765404.1"
                     /translation="MTDEARELAVPDALAGERLDRALARMFPEYSRTRLKDWLLDGFV
                     TVNGTARRPRDVVTGGEVVRLQPQASLGVTSQPEAIDLDVVFEDDELLVVNKPAGLVV
                     HPGAGNLRGTLMNGLLHRVPGLADLPRAGIVHRLDKDTTGLLLIGKTLPTHTALVRQM
                     AAREISRHYLAVCMGVLTGGGTIDAPIARHPIDRLRMSVQDDGKPAVTHYRVIKRFAA
                     H"
     gene            768..1565
                     /locus_tag="VFY27_07540"
     CDS             768..1565
                     /locus_tag="VFY27_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5765405.1"
                     /translation="MNGQARPPGAAAWDSLLRRFVWLMALLMFSAAILAGCAGSEEEE
                     NAMLRDVTKAYETAQKSMLNQNYRRAIEIYEALQARFPFSDLSKQIQLELMYAYYKSN
                     RQEQAIDAADTFMRENPTHSRVDYALYVKALAYFERDPGFLERLFNKEVDNRPPQDGV
                     LAISLLKRLVERYPASPYAEDARQRMIFLKNRLAAYENTVARYYLDRGAYVAALNRAK
                     GALEEYNGAESGDESLTIMIEAYEGLGMDELAADTRQVLQSNFPNES"
     gene            complement(1634..>2548)
                     /locus_tag="VFY27_07545"
     CDS             complement(1634..>2548)
                     /locus_tag="VFY27_07545"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5765406.1"
                     /translation="ELYADQSGVRPERSEIVPYLPTEQSVYEALVTGTRDYVLKNGFP
                     GVVLGLSGGIDSALILAIACDALGPESVRAVMMPFRYTSTMSQEDAARQAAGLGVRYD
                     VLPIEPMYDATVRQLAKIFAGREPDVTEENIQARCRGILLMAISNKTGSMLLSTGNKS
                     EMAVGYATLYGDMAGGFSPIKDCSKTLVYRLAHYRNSISEVIPRSVIERPPSAELRPN
                     QRDSDTLPPYEILDPILSAFIEEDLSVNEITARGFDREVVTRVLELVKRSEYKRRQAP
                     PGIRISRRAFGRDWRYPITSQYTFPETE"
BASE COUNT          484 a          780 c          790 g          494 t
ORIGIN      
        1 tgtgtgcggc gaagcgcttt atcacccggt aatgggtgac ggctggcttg ccatcgtcct
       61 gcacgctcat cctcaggcgg tcgatcgggt gccttgcaat gggagcgtcg atcgtaccgc
      121 cgccggttag cacgcccatg caaacggcca ggtagtggcg cgagatttca cgcgcggcca
      181 tctgccgcac cagcgccgtg tgcgtgggca gggtcttgcc gatcagcagg agcccggtcg
      241 tgtccttgtc gagacgatgc acgatcccgg cgcgaggaag atcggcaaga ccgggcacgc
      301 ggtgcagcag accgttcatc agggtccctc gcaggtttcc cgcgccggga tgcacgacca
      361 gtccggccgg cttgttcacc accagcaatt cgtcgtcctc gaaaacgaca tcgagatcga
      421 tggcctcggg ctgactggta acgccaagcg acgcctgcgg ctgcaagcgg acgacttcgc
      481 ctccggtcac cacgtcccgc ggacgcctgg ccgtgccgtt cacggtgacg aagccatcca
      541 gcagccagtc tttcagccgg gtgcgggaat attcgggaaa catccgcgcc agcgcccggt
      601 ccaaacgttc tccggcaagt gcatcgggca cagccagttc ccgggcttcg tcagtcatgg
      661 ggtatcagga tcatcgaaat gggcctcctg caagtttacg gtatactccc gcgatttcgc
      721 gattacgcaa cgtccttgaa tagatgattt ccaaccgatc cgcatccatg aacggccagg
      781 cccgccctcc tggcgctgct gcatgggatt ccctccttcg ccggttcgta tggctgatgg
      841 cgctgttgat gttcagcgcg gctatccttg caggttgcgc aggctccgaa gaagaagaaa
      901 acgcgatgct tcgcgacgtc acgaaggcct acgagacggc gcagaaatca atgctgaacc
      961 agaactaccg gcgcgcgatc gagatttacg aagcgctgca ggcaaggttt cccttcagcg
     1021 acctgagcaa gcagatacag ctcgagctga tgtatgccta ttacaagagc aaccgccagg
     1081 aacaggctat cgatgccgca gacactttca tgcgggagaa cccgacccac tcgcgcgtag
     1141 actacgccct ctacgtaaag gcactggcct acttcgagcg cgaccccggg ttcctcgagc
     1201 gactgttcaa caaggaagtg gacaatcggc cgccgcagga tggagtcctt gccatttctt
     1261 tgctcaagcg gctggtcgag aggtatcccg ccagcccgta cgccgaggac gccaggcagc
     1321 gcatgatctt cctgaagaac cgtctggccg catatgagaa cacggttgcc aggtattacc
     1381 tcgatcgcgg cgcttatgtc gcggcgctca atcgggccaa gggtgcgctg gaagaataca
     1441 acggcgccga gagcggtgac gagtcgctca cgatcatgat cgaggcctat gaaggtctcg
     1501 gcatggatga actggcggca gacacgcggc aggtcctgca gtccaacttc ccgaacgaaa
     1561 gctagagttg taggagcccg ccatgcgagc gagttcgctt aagcatatcg cacccgcctg
     1621 tcgacgtggg ctactactcg gtttcgggga aggtgtactg cgacgtgatg ggatagcgcc
     1681 agtcgcggcc gaaggcgcgg cggctgatgc gtatcccggg gggcgcctgg cggcgtttgt
     1741 attcgctgcg cttgaccagt tccagcaccc gcgtgacaac ttccctgtcg aagccgcgcg
     1801 ccgtgatctc gttcaccgac aggtcttcct cgatgaaagc agaaagaatc gggtcgagga
     1861 tttcgtaggg cggcaaggta tcgctgtcac gctggttcgg gcgcagttcc gccgaaggcg
     1921 gacgttctat gacgcttcgc ggtatcacct cgcttatgga attgcggtag tgcgcgagcc
     1981 ggtacaccag ggtcttgctg caatccttga tgggtgaaaa accgccggcc atgtcgccat
     2041 agagggtggc gtagcccacc gccatctcgc tcttgtttcc ggtggacagg agcatgctgc
     2101 cggtcttgtt cgagatcgcc atcagcagga tgccacggca gcgcgcctgg atgttttctt
     2161 ccgtcacgtc aggctcgcgg ccggcgaata tcttcgccag ctggcgcacg gtcgcgtcgt
     2221 acatcggttc tatcggcagg acgtcgtagc gcactcccag gccggccgcc tgtcgcgcgg
     2281 cgtcttcctg gctcatcgtg ctggtgtagc ggaatggcat catgactgcc cgcacgctct
     2341 ccgggcccag cgcgtcgcag gcaatcgcca ggatcaacgc cgagtctatg ccgccggaaa
     2401 gacccagcac gacgccgggg aagccatttt tcaggacgta gtcgcgggtt ccggtgacaa
     2461 gtgcttcata gacgctttgt tctgttggca gatagggcac gatctccgag cgttccggcc
     2521 tgacaccgga ttggtccgcg tacagctc
//
LOCUS       DASUOM010000360         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_13249,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000360 DASUOM010000000
VERSION     DASUOM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13249"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFY27_07550"
     CDS             complement(<1..504)
                     /locus_tag="VFY27_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEX5765407.1"
                     /translation="MTSSHRPVYEVTLSIDRAVLDEFDAWLEQHIREMLELPGFISAE
                     TFETEANSPDRAGRSVQYLLESDDALEAYLAGPAQEMRRAGALRFGDELEVSRRVLRP
                     ANAPERVARPPEVCLNCQALLQGQYCARCGQRARGRLISIWELVRDAVGDLFELDSRL
                     WQTIIPLI"
     gene            complement(517..3090)
                     /locus_tag="VFY27_07555"
     CDS             complement(517..3090)
                     /locus_tag="VFY27_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5765408.1"
                     /translation="MSSFLVSLSWFLLFFGGAIYLAYNRVNLFAATVAAGAALLAYTI
                     FGSGSFLWLVFLWLLFGLMVVPNLRDFRREKITRPALNIYRTMLPSMSDTERDALEAG
                     NVWWDGELFSGMPDWERLMSFPAPKLSDEEQAFLDGPCEELCRMLDDWEISHERGDMS
                     PEVWQFIKKHRFFAMIIPRQYGGLEFSAYANAMVISKLASRSTTASSTVGVPNSLGPA
                     ELLLHYGTEEQKRRYLPGLASGEEIPCFALTSPQAGSDAAAIIDSGIVCKGEWEGEEI
                     IGIRLNWDKRYITLAPVATVLGLAFKLYDPDHLIGVEEEYGITAALIPTSTPGVHTGR
                     RHLPLSTPFQNGPTSGKDVFVPLDFIIGGREMAGKGWKMLVELLSVGRAITLPSTAAG
                     GGQAAAYATGAYAQLRKQFSLPIARFEGVGEALTRIAGHTYIMNAAVSVTSGAIDQGE
                     KPAVPAAILKYHCTELGRKVANDAMDVHGGKGIMMGPSNYLGRSYMATPIAITVEGAN
                     ILTRSLIIFGQGAIRCHPFVLRELYAAQDEDAERGLLEFDDALFSHVGYAISNLARAF
                     FLALTHAKFSRVPLNTPTRRYYQNINRYSAAFALASDFAMLTLGGKLKQKELLSARLG
                     DVLSCMYLASAVLKHFENQGRPASDLPLVEWAVRTLMYQAQEQLHSFLRNFPNRPVAF
                     FLRCFIFPRGRTYSSPADELGRKIVDLITRSGEVRERLSQQAYTTIEPGNPIGLLQEA
                     LSLSESLAPLEQRLRQARKEGLIRAEYLGHQIEEAATAEVISDDEAVRLRGYHRKLRA
                     LMAVDDFAANDIGRVGRHGQQDDEAGDAATPSARSAAKSKARAKKKTSATRKKATPGS
                     D"
     gene            complement(3103..4080)
                     /locus_tag="VFY27_07560"
     CDS             complement(3103..4080)
                     /locus_tag="VFY27_07560"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5765409.1"
                     /translation="MKAPVRVAITGAAGQIGYQLAFRVASGQMLGADQPVILQLLEIP
                     PALPALKGVVMELDDCAFAPLADIVATDDANKVFKDANFALLVGARPRGPGMERNDLL
                     EANAQIFSTQGKAINESANRDIRVLVVGNPANTNALIARANAPDIDPGQFTAMTRLDH
                     NRAIAQLAARTGAHVTDVDRMIIWGNHSATQYPDIHHATVRGVPATDLVEDAWYTEEF
                     IPAVQQRGAAVIKARGASSAASAASAAIDHMHDWALGSKEDDWVSMAVPADGSYGVGE
                     GIVYSFPVRCHDGSYEIVRDLEISDFSRERMQVTEAELKSERDAIEHLL"
     gene            4371..4556
                     /locus_tag="VFY27_07565"
     CDS             4371..4556
                     /locus_tag="VFY27_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765410.1"
                     /translation="MRTRIGDVPDSLISVSLLLLFLILMAASVAGQARANLPQEARAD
                     GCFAASVVQPKAADGTE"
BASE COUNT          818 a         1417 c         1441 g          933 t
ORIGIN      
        1 tatgagcggg atgatcgtct gccatagccg ggagtcgagt tcgaacaggt cccccactgc
       61 gtcgcggacc agttcccata tgctgataag gcggccccgg gcacgttgcc cgcagcgagc
      121 gcagtattgt ccttgcagca atgcctggca gttcaggcag acctcgggtg gccgggctac
      181 gcgttcggga gcattcgccg gacgcaatac gcgtcggctc acctccagct cgtcaccgaa
      241 ccgcaatgca ccggcgcggc gcatctcttg cgcggggcct gccaggtacg cctccagggc
      301 gtcgtcgctc tccagcaggt attgcaccga ccggccggcc ctgtcgggcg agttcgcttc
      361 ggtttcgaag gtttccgcgc tgatgaaccc gggcaactcg agcatttccc ggatgtgctg
      421 ttcgagccag gcatcgaatt catcgaggac cgcccggtcg atactcaggg ttacttcgta
      481 gacaggcctg tgagaggagg tcataggctg ccagggtcag tcgcttccgg gagttgcctt
      541 ctttctcgtt gcggaagttt ttttcttcgc cctcgccttc gacttcgcgg cgcttcgggc
      601 ggatggtgtc gccgcatccc ccgcttcgtc gtcctgctgg ccgtgtcgcc cgacgcggcc
      661 gatgtcgttc gctgcgaaat cgtccacagc catcaatgcg cgcagtttcc ggtggtaacc
      721 acggagacgc acggcttcgt cgtcactgat gacctcggcg gtcgccgctt cctcgatctg
      781 gtgaccgagg tattcagccc ggattaaccc ctctttgcgc gcctgtcgaa gcctctgttc
      841 gagcggcgcc aggctttcgg acaatgacag ggcttcctgc agtagtccga tagggttccc
      901 gggctcaatc gtggtgtatg cctgctggct caggcgctcg cgtacctcgc cactccgggt
      961 gatgagatcg acgatcttcc ggccaagttc atcggcgggt gatgagtatg tgcggccccg
     1021 gggaaagatg aagcagcgaa ggaagaaagc gaccggccga ttggggaaat tacgcaggaa
     1081 actgtgcaac tgctcctggg cctggtacat cagcgtgcgg accgcccact cgaccagcgg
     1141 caggtccgat gccggccgtc cctggttttc gaaatgcttc agtaccgccg acgccaggta
     1201 catgcaactc agtacgtcac ccagtctcgc ggacagcagt tccttctgct tcaattttcc
     1261 gccaagcgtc agcatcgcga agtcggaagc cagcgcgaac gctgcgctgt agcgattgat
     1321 gttctggtaa tagcgcctgg tcggggtgtt caggggcacg cggctgaact tcgcgtgcgt
     1381 cagcgcaagg aagaatgctc gcgcaaggtt gctgatggca taaccgacat gagagaacag
     1441 cgcgtcgtcg aattccagca gcccgcgctc ggcatcctcg tcctgcgccg cataaagttc
     1501 ccgcagtacg aacggatggc agcgtatcgc gccctggccg aagatgatga ggcttcgggt
     1561 aaggatatta gcgccctcga ctgtgatcgc tatcggagtc gccatgtagc tgcggccgag
     1621 gtaattgctg ggccccatca tgatgccttt gccgccgtgg acgtccatag cgtcgttggc
     1681 aaccttccgg ccgagctcgg tgcagtgata tttcaggatc gccgcgggca ctgccggctt
     1741 ctcgccctgg tcgatggcgc cggacgttac cgagacagcg gcattcatga tgtaagtgtg
     1801 accggcgatg cgcgtcagtg cttcgccgac accttcgaac ctggcaatcg gaaggctgaa
     1861 ctgtttgcgc aactgcgcgt aggcaccggt agcataggcc gccgcctgtc cgccacccgc
     1921 ggctgtcgag gggagcgtga tcgcgcgacc caccgacaac agctcgacca gcattttcca
     1981 gcctttcccg gccatctccc tgccgccgat tatgaagtcc agcggcacga atacgtcctt
     2041 gccgctcgta gggccgttct ggaacggcgt gctgagcggc aggtgccgtc gccctgtgtg
     2101 gacgcccggg gttgaagtcg ggataagcgc ggcggtgatg ccgtactctt cctcgacgcc
     2161 aatcaggtgg tccgggtcgt agagcttgaa ggcgaggccc aggacggtag caaccggtgc
     2221 gagcgtgatg taacgcttgt cccagttgag cctgatgcca atgatttctt ccccctccca
     2281 ttcccccttg caaacgatgc cgctgtcgat gattgcagcg gcatcggacc cggcctgggg
     2341 ggacgtgagc gcaaagcagg gaatctcctc ccccgaagcc agccccggca ggtagcgccg
     2401 cttctgttcc tccgtgccgt aatgcaggag caattcggcc ggccccagcg agttcggcac
     2461 tcccacagtg gacgacgcgg tcgtgctgcg gcttgcgagc ttcgagatga ccatagcgtt
     2521 cgcatacgca gaaaattcca gtccgccgta ctgcctgggg atgatcatcg cgaagaaccg
     2581 gtgcttcttg atgaattgcc agacctccgg gctcatgtcg ccgcgctcgt gggatatctc
     2641 ccagtcgtcg agcatcctgc aaagctcttc gcacggaccg tcgaggaaag cctgttcctc
     2701 atccgagagc ttcggcgccg gaaacgacat cagccgttcc cagtcgggca ttccggaaaa
     2761 cagttcgccg tcccaccaga cgttgcccgc ctcgagtgcg tcccgctccg tgtcggacat
     2821 cgacggcagc atcgtgcgat agatgttcaa ggctggccgg gtgatcttct cgcggcggaa
     2881 gtcgcggaga ttgggaacga ccatcagtcc gaacagcagc cagagaaaaa ccagccagag
     2941 aaaggatccg gatccgaaaa tcgtgtacgc gagcaacgcg gcgcctgccg caacggtcgc
     3001 cgcgaacagg ttgaccctgt tgtacgcgag gtagatggcg ccgccgaaaa acagcaggaa
     3061 ccaggaaaga cttacgagga aactggacat ttagttcctt tgctacagca ggtgctcgat
     3121 cgcgtcgcgc tcgctcttca gctctgcttc cgtcacctgc atgcgttcgc ggctgaagtc
     3181 gctgatttca aggtcgcgca cgatctcata gcttccatcg tggcaccgca cgggaaatga
     3241 atacacgata ccttcgccaa ccccgtagct gccgtcggcc ggcaccgcca tgctcaccca
     3301 gtcgtcctct ttcgagccca acgcccagtc gtgcatgtgg tcgattgccg cggacgcggc
     3361 cgatgctgca gacgatgcac cgcgggcctt gatgactgcg gctccccgct gttgcactgc
     3421 cgggatgaat tcttccgtat accacgcgtc ttccaccagg tcggtcgcag gcacgccgcg
     3481 cacggtggcg tggtgaatgt cgggatactg agttgccgag tggttgcccc agattatcat
     3541 cctgtcgaca tccgtgacgt gtgctcccgt tcttgcagcg agttgtgcaa tggccctgtt
     3601 gtggtccagc cgggtcatcg ccgtaaattg ccccgggtcg atgtccggtg cgttcgcgcg
     3661 ggcgatcagt gcgttcgtgt tggcaggatt tcccaccacc aggacgcgga tgtcgcgatt
     3721 cgcgctctcg ttgatcgcct taccctgcgt ggaaaagatt tgcgcgttcg cctcgaggag
     3781 atcgttgcgt tccatccccg ggccgcgggg gcgagcacct accagcagcg caaagtttgc
     3841 atccttgaaa accttgttcg catcatcagt cgccacgatg tccgcgaggg gtgcgaaagc
     3901 acagtcgtcg agttccatca ccacgccctt cagcgccgga agcgcgggcg gtatctcgag
     3961 taattgaagg atcaccggct ggtccgcgcc gagcatctgt cctgatgcga cacgaaacgc
     4021 gagctgatag ccgatctggc cggcggcgcc ggtgatggcg acgcgaacgg gtgctttcat
     4081 tccatgacct tagttgatgg tgcgacgatt gactggccgt ggaggcgcgg tctccaggct
     4141 gcgctcactg tacacaagcc gggagagagg ccgcaacgat cgctgtctcc gttgccggcc
     4201 gcaggcggtg cttgtcccgg gaggacttcc ggaagctgac accgcgcctg cggcttttag
     4261 ggccttttag cttcgatggc ttgcattctg cccatttggt gttatagttc actataacac
     4321 taggtcacca acagagggcg agggtgggcc tcgccccagt ggagtgtgag atgagaacca
     4381 gaattggcga tgttccggac agcctgatca gtgtttcgct gctattgctg ttcctgatcc
     4441 tgatggctgc ctcggtagcc ggccaggcgc gtgcgaacct gccacaggag gcgcgggcgg
     4501 acggttgctt cgcggcctcg gtggttcagc cgaaggcggc cgacggtacg gaatgaggaa
     4561 agccgggtca ttcgaagcgg gcgcggggcc ggacaccatg agccgggcg
//
LOCUS       DASUOM010000361         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_520142,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000361 DASUOM010000000
VERSION     DASUOM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520142"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFY27_07570"
     CDS             complement(<1..885)
                     /locus_tag="VFY27_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5765411.1"
                     /translation="MSDSRFRINPVAAAVSTALLIGSGHSALAQNSADDTIEEVTVTG
                     IRSSLRSSMHVKRNSTGVVDAISAEDIGKFPDTNLAESMQRIAGVSIDRLNGEGNQVT
                     VRGFGPGYNLVTLNGRTIPTTEVPLIGTQNSFTGAQGRSFDFSNIASEGVSGLEVIKT
                     GKATLPSGGIGATVNIQTLRPLEGGGNQGTFGAKALVDSSVAEGDDVTPELTGLYSWV
                     SDSETLGVTVFGAYSQRDSGGASAYINDYAFGYGPGLASSFLRAGGTTTLTNPPPDGQ
                     MWAIPQDSRNDFSNISRER"
     gene            complement(1406..2056)
                     /locus_tag="VFY27_07575"
     CDS             complement(1406..2056)
                     /locus_tag="VFY27_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765412.1"
                     /translation="MVVLGGLALLEWPWGNRLPGKPLEIEVSFPRDEREPVAETPADP
                     VEQPAAVEEVVAPEPPRSAAVEEPQPPAAPAGQPQVDWQKSLERASAKTVEEHAGMDS
                     LHPEFDELRRIAATRYAEPRTGKPPPIWENVEKDVTGRTLLRSGNCFQVLDDPNVGNR
                     YAFETFEQHLVFCDWAKAPRQEFPWIRTIVARYQYLREPDGGAAGPVGKIVVEAGR"
     gene            2229..2867
                     /locus_tag="VFY27_07580"
     CDS             2229..2867
                     /locus_tag="VFY27_07580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5765413.1"
                     /translation="MELLVEEAIDRYAFLNTSPEPAYLQALEERTLQEMDDPQMLTGR
                     VEGRLLKFIVQLCQPRFVLEVGTFTGYSALSMAEGLGEGGRILTCEINPEAQKMARES
                     IDASPFADRIEIALGPALDTIRALDRQIDLSFIDADKERYPDYYEEIVQRTRPGGILI
                     FDNMLWSGRVVDPRDDITRAIAQLNETITADDRVENILLTVRDGVQLVRKLW"
     gene            complement(2891..>3295)
                     /locus_tag="VFY27_07585"
     CDS             complement(2891..>3295)
                     /locus_tag="VFY27_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765414.1"
                     /translation="ACSLAHGKALAAFIGEEEVQRIALGEAGLDLTAAEQAMVDYARK
                     VATDASKITSGDVAILRSHGLSDPEIFDIAAVAAGRAFFTKILDSLGVEADSSFLSLS
                     EDMRETLTVGRPIDFRKTETLAAEASTLGAVG"
BASE COUNT          624 a         1015 c         1014 g          642 t
ORIGIN      
        1 gcgctcgcga ctgatattgg agaaatcgtt gcggctgtcc tgaggtattg cccacatctg
       61 gccgtcaggc ggcggattgg tcagcgtggt cgtgccgcct gcacgcagga agctagacgc
      121 cagtccggga ccgtagccga acgcatagtc gttgatgtag gcactggcac cgccggagtc
      181 gcgctgcgaa tacgcaccga ataccgtaac gcctaaggtt tcgctatcac tcacccagct
      241 gtataacccg gtcagctcgg gcgtaacgtc gtcaccttcg gcgacgctgg agtccacgag
      301 cgccttggcg ccaaatgtgc cctggttacc cccgccttca agcggcctga gcgtttggat
      361 attgacggtc gcgccgatac cgccggaagg cagtgtggcc ttgccggtct tgattacttc
      421 gaggccggag acgccttcag acgcgatgtt cgagaagtcg aagctgcggc cctgcgcgcc
      481 ggtaaagctg ttctgggtgc cgatcagcgg cacctcggtg gtcgggatcg tgcggccgtt
      541 cagggtgacg aggttgtagc cggggccaaa cccgcgaacc gtaacctggt tgccttcgcc
      601 gttgagccgg tcgatcgaga caccggcgat gcgctgcatc gactcggcga ggttggtatc
      661 cgggaacttg ccgatatcct cggcagagat ggcatcgacg acgcccgtgg agttgcgctt
      721 gacgtgcatc gaactgcgca agctgctgcg tatgccggtc acagtgactt cttcgatcgt
      781 atcgtcggca ctgttctggg caagtgccga atgacctgac ccgatcaaca aagccgtgct
      841 tacggcggct gcgacgggat taattctgaa tctggaatcg ctcatcgatc gttccccctg
      901 ctgtcatgtg aatgactaat gcctgctctc gtactgtcac gtaccgccgg tcgagacttc
      961 agggggacta ccgccgtcgt gtcgcaacat gcaccagtcg aacgccacat cgatcgaaaa
     1021 ccgatgcggt attgggtgct gaatggtcgt tacacgactg ggccttggat ctgagcttct
     1081 cggatacccc ctgcgcattt cgataccgct tcctgagtcc tgccgaagcg gcttgaaggt
     1141 cccggcagcc cccctcagga acgaagtttt aaaggcacga cgggacgtat gacatgttct
     1201 tcgcgcggtg agaatttgtc aagaataact tggtagcgct agcagtagat gacgcaactc
     1261 gacctaagtg gttgttttct agaagccggg gatgaccaga ttgggatttc ccgcgcttgc
     1321 tgcccggtag cgataacaaa tggagttcgc cggcagacgg ttttgcgtgt aacaaaccgg
     1381 gccacaacgc gccgcttcgc caacatcacc tcccggcttc gactacgatc tttccaaccg
     1441 gtcccgccgc accgccatcg ggctcccgga ggtactggta acgggcgacg atcgtcctga
     1501 tccaggggaa ctcctggcgg ggcgctttcg cccagtcgca gaaaacgagg tgctgctcga
     1561 aggtctcgaa cgcgtagcga ttgccgacgt tggggtcgtc cagtacctgg aagcagttgc
     1621 cactacggag gagcgtacgc ccggtaacgt ccttttccac gttctcccaa atcggcggag
     1681 gcttgccggt acggggttcg gcatacctcg ttgcggcgat gcgccgcagt tcatcgaact
     1741 ccggatgcaa cgaatccatc ccggcgtgtt cttcgacggt tttcgccgat gcgcgttcga
     1801 gagacttttg ccagtcgacc tgcggctgcc ccgccggggc agcgggaggc tgcggctcct
     1861 cgactgctgc cgaccgcggc ggttcaggcg cgacgacttc ttctaccgcc gccggttgtt
     1921 ccactggatc ggccggcgtt tcggcaactg gctcacgctc atcccgggga aaagacactt
     1981 cgatctcgag aggcttcccc ggcaggcggt tgccccatgg ccattcgagc agcgccagcc
     2041 ctcccaggac gaccactgcc gacgccgcgg tgcaacagac caaccgtcgg tgccccgggc
     2101 ggatggagcc tgaggcggac tggcaggcca atagacgatc cacgtgaacc actgagcgct
     2161 tcctcgaggt tgcctggcac ggcgtccgac tacactatga tgcgcgagag tcaccaggag
     2221 aatcgatcat ggagttgctc gtcgaagaag ccattgaccg ctacgccttc ctgaacacca
     2281 gcccggagcc tgcgtacctc caggcgctcg aggaacgaac gctgcaagag atggacgacc
     2341 cgcagatgct cacggggcga gtggagggcc ggctcctgaa gtttatcgtg cagctatgcc
     2401 agccccgctt cgtcctggaa gtcgggacgt tcaccggcta ctccgcgttg tcaatggccg
     2461 aaggactcgg cgagggcggc cgcatcctga cctgcgagat aaaccccgag gcgcagaaaa
     2521 tggcccgcga aagtattgat gcaagtccct tcgctgaccg catcgaaata gcactggggc
     2581 ccgcactcga caccattcgc gcgctcgacc gccagatcga tctttcgttc atcgacgccg
     2641 acaaggagcg ttacccggac tactacgaag agatcgtgca gcgcacccgg cctggcggca
     2701 tcctcatatt cgacaacatg ctgtggtcag gacgcgtcgt ggatcctcgc gacgacatta
     2761 cgcgggcaat tgcgcagttg aacgagacga tcaccgccga tgaccgcgtc gagaacattc
     2821 tgctgacggt gcgcgacggt gtgcagctgg tgcgaaaact ctggtgaagg tctgcccggc
     2881 cggctgtggc ctacccgacc gcccccagcg ttgatgcctc agcagccagc gtctcggtct
     2941 tgcgaaagtc gataggccgg ccgacggtaa gggtctccct catgtcttca cttaaggaga
     3001 ggaagctgga atcggcctcg accccgaggc tgtccaggat cttggtgaaa aaggcgcgac
     3061 ctgccgcgac cgccgcaatg tcgaagatct cgggatcgga gaggccgtgt gagcggagta
     3121 tggccacgtc gccggatgtg atcttcgagg catcggtcgc taccttgcgg gcgtaatcga
     3181 ccattgcctg ctccgcggcc gtcaggtcta aaccggcttc gccgagggcg attcgttgca
     3241 cctcctcctc gccgatgaaa gccgccagcg ccttgccatg agccagtgag catgc
//
LOCUS       DASUOM010000362         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_16963,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000362 DASUOM010000000
VERSION     DASUOM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16963"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1343
                     /locus_tag="VFY27_07590"
     CDS             186..1343
                     /locus_tag="VFY27_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX5765415.1"
                     /translation="MVEFDFGLGDDVEMLRQTLRDFTRDQVEPQAAEIDRSNRFPRDL
                     WPKLGELGLLGITVEEEFGGSGLGYLEHVVAMEEISRASASIGLSYGAHSNLCVNQLR
                     RWGSDEQKRQYLPKLVAGEHVGALAMSEHGAGSDVMAMRTTATKKDDHYLLSGSKMWI
                     TNAPEADVVIIYANTDDGSGKPRLSAFIVESGVPGFSTAQKLDKLGMRGSDTGEIVLD
                     DCVVPERNLLGEEGQGASILMSGLDYERLVLAGGPLGIMRACLELVMPYVHDRKQFGQ
                     PIGTFQLMQGKIADMYTIMNACRSYVYAVARAADRSQTTRFDAAGCILLAAEKATWMA
                     GETIQALGGNGYINEYPAGRLWRDAKLYEIGAGTSEIRRMLIGRELFNATG"
     gene            1356..2966
                     /locus_tag="VFY27_07595"
     CDS             1356..2966
                     /locus_tag="VFY27_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5765416.1"
                     /translation="MPKIKTKVKSSSEEYEKNAAANMNLARELRELQETISKGGSERA
                     REKHLSRGKLLPRDRIRSLVDRGAPFLEVGQLAAYGMYDNEVPAAGIVTGIGQVSEVD
                     CMIVANDATVKGGTYYPLTVKKHLRAQEIAAQNALPCVYLVDSGGAFLPKQDEVFPDR
                     DHFGRIFFNQARMSAQGIPQIAAVLGSCTAGGAYVPAMCDEAIIVRNQGTIFLGGPPL
                     VKAATGEVVDAESLGGGDVHSRISGVTDYLAASDMEALATVREIVSNLNRPRPAPLQL
                     REPAEPAYPAEELYGVVSRDFRVPYDVREVIARLVDGSEFQEFKALYGPTLVCGFAHL
                     YGYPIGIIANNGILFSESAQKGAHFIQLCNRRGIPLVFLQNIVGFMVGKKYEQAGIAK
                     DGAKMVNAVATSSVAKFTVIIGGSFGAGNYAMCGRAYGPRFLWMWPNARISVMGGDQA
                     ALVLSTVRREGLEARGEEWPDAEQKKFEDEIRKQYDSQGHPYYASARLWDDGVIDPAD
                     TRRVLGQAVSAAVNRLRDEDMPHGIFRM"
     gene            3048..>3761
                     /locus_tag="VFY27_07600"
     CDS             3048..>3761
                     /locus_tag="VFY27_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5765417.1"
                     /translation="MNELDGKTLFITGGSRGIGLAIALRAARDGANVIIAAKTAEPHP
                     KLPGTIYTAAEEIEAAGGNALPVVCDIRSEEQVEEAVRQGVEKFGGIDICVNNASAIA
                     LEPTSRISMRRFDLMHQVNSRGTFLVSKTCLPHLEKASNPHILNLSPPLEMEEKWFAP
                     HVAYSIAKYGMSLCVLGMAGEFRKKGIAVNALWPRTTIATAAVQNLLGGDEIVRRSRK
                     PAILGDAAHVILSKPSREFT"
BASE COUNT          763 a         1118 c         1222 g          658 t
ORIGIN      
        1 tgtgaagaac atcgcactcg cccgcacggc gggctcctac ccgcccgaat aagaccggtg
       61 ccgagctgcc tgatgccgcg cttcggtacc gctcgaatgg tattcaggcg ccgcgctatt
      121 caccggagaa tctcccttgc ccgcaagcgt cgcggatgtg gggaaccggc tccggaggga
      181 acttgatggt cgaatttgat tttgggctcg gtgacgacgt tgagatgctg cggcagacct
      241 tgcgcgactt cacacgtgac caggtcgagc cgcaagcggc cgagattgac cggtcaaacc
      301 gcttcccgcg ggatctgtgg ccgaagctcg gcgaactggg gctccttggt atcacagtcg
      361 aagaggagtt cggcgggtcc gggctcggtt atctcgagca tgtcgtcgcg atggaggaaa
      421 tcagccgcgc gtcggcgtcg atcggcctgt cgtacggagc ccactccaac ctttgcgtga
      481 accagctgcg ccgctggggc agcgatgagc agaagcggca atacctgcca aagctcgttg
      541 ccggggaaca cgtaggggct ctcgccatga gcgagcacgg cgccgggtcc gacgtcatgg
      601 ccatgcgtac gaccgcgacg aaaaaggatg atcactacct cctgtccggc agtaaaatgt
      661 ggatcacgaa tgcgccggaa gccgacgtcg tgattattta cgcgaatacc gacgacggtt
      721 cggggaaacc tcgcttgagc gctttcatcg tggaaagcgg tgtgccgggt ttctcgacgg
      781 cgcagaagct ggacaagctc gggatgcgcg gatccgacac cggtgagatc gtgctggacg
      841 attgcgtcgt tccggagcgg aacctgctgg gcgaggaagg gcagggcgcc agcattctca
      901 tgagcggact ggattacgag aggctggtgc tggccggcgg tccgctcggc atcatgcggg
      961 cgtgcctcga gctcgtcatg ccgtatgtgc acgatcgcaa gcagttcggg caacccatag
     1021 gcaccttcca gctcatgcag ggcaagatcg ccgacatgta cacgatcatg aacgcctgcc
     1081 ggtcttacgt gtatgccgtc gcgcgtgccg ccgaccgctc gcagacgacg cgattcgacg
     1141 cggcgggctg catcctgctg gccgcggaga aagcgacctg gatggcaggc gaaaccatac
     1201 aggcactggg tggaaacggt tatattaacg agtatccagc cggacgcctg tggcgcgacg
     1261 ccaagctcta cgagatcggc gccggcacca gcgaaatccg ccgcatgctg atcggacgcg
     1321 agctgttcaa cgccaccggg tgatcgtctt tccgaatgcc aaaaatcaag accaaggtaa
     1381 agagctcgtc ggaagagtac gagaagaatg ccgccgccaa catgaatctc gcccgggagc
     1441 tgcgagaact tcaggagacg atctcgaagg gcggcagcga acgcgcgcgg gagaaacatc
     1501 tttcgcgcgg caagctgttg ccccgggacc ggattcgcag cctcgttgac aggggcgcac
     1561 ccttcctcga agtgggccag ctagccgcgt acggcatgta cgacaatgaa gtgccggccg
     1621 ccgggattgt gaccgggatc ggccaggtaa gcgaagtcga ctgcatgatc gtcgcaaacg
     1681 atgcgacggt aaaaggcgga acctactacc ccctcaccgt caagaagcac ctgcgagcac
     1741 aggaaatcgc ggcgcaaaat gcactgcctt gcgtctacct cgtcgactcc ggcggagctt
     1801 tcctgccgaa acaggacgag gtcttccccg accgcgatca cttcggccgt attttcttca
     1861 accaggcgcg gatgtcggca caaggtatcc cgcagattgc tgccgtgctg ggatcgtgca
     1921 ccgccggcgg cgcctacgtg cctgcgatgt gcgacgaagc gatcattgtc cgcaaccagg
     1981 gcacgatttt tcttggcggt cctccgctgg tcaaagcagc aaccggcgag gtcgtcgatg
     2041 cggagtccct gggcggcgga gacgtgcaca gcaggatatc cggcgtgacg gactacctgg
     2101 ccgcgagcga catggaagcg ctggcaaccg tgcgggaaat cgtgtcgaac ctgaaccgtc
     2161 cccgtccggc accgttacag cttcgcgaac cggcagaacc cgcgtatccg gccgaggagt
     2221 tatacggcgt cgtttcgcgc gacttccgtg tcccctacga cgtgcgggaa gtgatagcac
     2281 ggttggtgga tggctcggag ttccaggaat tcaaggcgct gtacgggcca acgctcgtgt
     2341 gcgggttcgc tcacctctat ggatacccga tcggcatcat tgcgaacaat ggcatcctgt
     2401 tctccgagtc ggcgcagaaa ggtgcgcact tcatccagct gtgtaaccgg cgcggcattc
     2461 ctctggtatt cctgcagaac atcgtcggct tcatggttgg caagaaatac gaacaggccg
     2521 gaattgccaa ggacggcgca aagatggtca acgcggtggc cacgtcctcg gttgccaagt
     2581 tcacggtaat tatcggcggc tccttcggcg cgggtaatta cgcgatgtgc gggcgcgcct
     2641 acggaccgcg cttcctctgg atgtggccga acgcgcgtat ctcggtcatg ggaggggacc
     2701 aggctgcact ggtgctttcc acggtccggc gcgaggggct ggaggcgcgg ggcgaggagt
     2761 ggccggacgc ggaacagaag aaattcgagg atgagattcg taaacagtac gattcgcagg
     2821 gccaccctta ctatgcaagc gcaaggctgt gggacgacgg cgtcatcgat ccggcagaca
     2881 cacggcgcgt gctcgggcaa gccgtttccg ctgcggtcaa tcgcctgcgc gacgaggaca
     2941 tgccccacgg catttttcgc atgtagccct tgcctggact ggcgaccata gcgcagagct
     3001 tcggcagtcc gcgtatcatg ctccattgaa aacaagtggg cagcagcatg aatgagctcg
     3061 acggcaaaac actgttcatc accggcggca gccggggcat cggccttgct attgcgctgc
     3121 gcgccgcaag ggacggtgcg aacgtcatca ttgcagcgaa gaccgctgaa cctcatccca
     3181 aactgccggg aacgatttac acggcggcgg aagagatcga ggcagccggg gggaacgcgc
     3241 tgccggtcgt ctgcgacata cgctccgagg agcaggtgga ggaggcggtg cgccagggcg
     3301 tcgagaaatt cggcggcatc gacatctgcg taaacaatgc cagcgcgatc gcgctggagc
     3361 ccacctcgcg catctcgatg cgccgcttcg acctgatgca ccaggtgaac agccggggca
     3421 cgttcctcgt gtcgaagacc tgcctgccgc acctggagaa ggcctctaac ccgcacatcc
     3481 tgaacctctc cccgccactg gagatggagg aaaagtggtt tgcgccgcac gttgcctact
     3541 cgatcgcgaa gtatggcatg agcctttgcg tgctcggcat ggccggcgag ttcaggaaga
     3601 agggaatagc cgtcaacgcg ctgtggccca ggacgaccat cgcaacagcc gctgtacaga
     3661 acctgctggg tggcgacgag atcgtccgcc ggtcgaggaa gcctgcaata ctgggcgatg
     3721 ccgcgcacgt gatcctttcg aagccgagcc gtgagttcac c
//
LOCUS       DASUOM010000363         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1598993,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000363 DASUOM010000000
VERSION     DASUOM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598993"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..771)
                     /gene="hisA"
                     /locus_tag="VFY27_07605"
     CDS             complement(31..771)
                     /gene="hisA"
                     /locus_tag="VFY27_07605"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5765418.1"
                     /translation="MRVIPAIDLKGGRCVRLYQGDFERETCYSKDPLEVARRFSEMGF
                     DHLHVVDLDGALSGEQRHRDVVSKIADQTQFRLQVGGGIRSREGVADWLDAGAARCVI
                     GSLAVTQPRTVAEWFREFGAERLVLALDVRTADDTPPVVATHGWTATSSLTLWDCVDA
                     YAGCSLQHVLCTDIGRDGAMTGPNTSLYSQFMRRYPEVELQASGGVRHGADLEALSKL
                     GCAAAITGRALLDGRISTEDVLPFLRNA"
     gene            complement(781..1380)
                     /gene="hisH"
                     /locus_tag="VFY27_07610"
     CDS             complement(781..1380)
                     /gene="hisH"
                     /locus_tag="VFY27_07610"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5765419.1"
                     /translation="MRADIAIIDSGGSNIASLSSALERLGASSIRTADANTIRSASHV
                     ILPGVGAAADGMRKLKRAELAEVIPRLTQPVLGICLGMHLLAQHSEEDDAECLGVLPA
                     AALRLEASPGLPVPNMGWCRVTKTAEHPLLHGIDSDSYFYFVHSYALPVDHFTVATAA
                     HATELTAVSSSRNFVATQFHPERSAAAGARLLANFLAMR"
     gene            complement(1377..2450)
                     /gene="hisB"
                     /locus_tag="VFY27_07615"
     CDS             complement(1377..2450)
                     /gene="hisB"
                     /locus_tag="VFY27_07615"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEX5765420.1"
                     /translation="MKKVLFIDRDGTLIEEPADNQVDALAKIRLVRDVVPALISLRDA
                     GFRFVMVTNQDGLGTESFPKQDFELCHRHVISIFESQGVTFDEIFICPHRSADGCGCR
                     KPNAGLLTRYLAESVLDRERSAVVGDRDTDLQLAKNIGIRGLLVRPTDGFEFSWCGIA
                     QTLKATTRLATVARETRETRIRVTVNLDASGPIHVDTGIGFYDHMLEQVARHGGFSMR
                     LECGGDLDVDEHHTVEDTAICLGEALRKALGDKRGIERYGFLLPMDESEARVSLDLSG
                     RPWFVFEGQFSRESVGGLPTELVPHFFRSFAESLGASLHMAVLGENAHHMVEACFKAV
                     GRALRQAIRQSGQEMPSTKGMLG"
     gene            complement(2452..>2733)
                     /locus_tag="VFY27_07620"
     CDS             complement(2452..>2733)
                     /locus_tag="VFY27_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765421.1"
                     /translation="RLAARKVLGERDRLAVALRQCPDVVKVWPSRSNFLLVRFKNFPE
                     AEERLRAHRVLVREFGHDAALGNCARISVGKPWENNLLLEALGTCAERS"
BASE COUNT          511 a          896 c          824 g          502 t
ORIGIN      
        1 cctggccgtc gcgaacatcg agacagggaa ttatgcgttt cgcaggaatg gcaatacgtc
       61 ctcggttgag atccggccat cgagcagcgc gcgccccgta atcgccgctg cgcaaccgag
      121 cttgctcagc gcctcgagat cggcaccatg gcgcacgccg ccggaagcct gcagttcgac
      181 ctccgggtaa cgccgcatga actggctgta caagctcgta ttgggtccgg tcatggcgcc
      241 atccctgcca atgtcggtac agaggacgtg ctgcaagctg cagccagcgt aggcgtcaac
      301 gcagtcccac agcgtaagcg aactcgtcgc cgtccagcca tgagtcgcga caacgggggg
      361 agtatcgtca gccgtacgca cgtcgagcgc caggacgagg cgttccgcgc cgaattcgcg
      421 aaaccactcc gcgaccgtgc gcggctgcgt cactgcgagg ctgccgatca cacagcgtgc
      481 ggccccggcg tcgagccagt cggcgactcc ttcacggctg cgaatgccgc cgccgacctg
      541 cagcctgaac tgcgtttggt cggcaatttt cgatacaacg tcgcgatgcc gttgttcgcc
      601 gctcagggcg ccgtcaagat cgacgacgtg caggtgatcg aaacccatct cactgaatcg
      661 ccgcgccacc tcgagagggt ctttggagta gcatgtctcc cgctcgaagt caccctggta
      721 aaggcgcacg cagcggcctc ccttcagatc gatagcggga atcacgcgca taaaacgatc
      781 tcagcgcatc gccaggaagt tcgcgagcag ccttgctccc gccgccgcgg aacgctccgg
      841 atggaactgc gtcgcgacga agttgcgcga ggaggaaacg gcagtcagtt ccgttgcgtg
      901 cgcggcggta gcgaccgtga aatggtcgac ggggagcgca tagctgtgca cgaagtagaa
      961 gtagctgtca ctgtcgatgc cgtgtaatag tggatgctcc gctgtctttg tcacccggca
     1021 ccagcccatg ttcggcaccg gcaggcccgg cgatgcttca agccgaagag ccgcggcagg
     1081 cagcaccccc aggcattccg cgtcatcctc ttccgaatgt tgcgcaagca ggtgcatgcc
     1141 caggcagata ccgagtaccg gttgtgtcag gcgtggaatg acttcggcca gttccgcgcg
     1201 cttgagcttg cgcatgccgt ccgctgcagc accgacgccg gggagaatga cgtggcttgc
     1261 cgagcggatc gtgttcgcgt cggctgtgcg aatgctgctt gcccccagcc gctccagcgc
     1321 agagctcagc gacgcgatgt tcgagccgcc gctgtcgata atagcgatgt ccgctctcac
     1381 cccagcatcc ccttggtcga gggcatttcc tgcccgctct gccgtatcgc ctggcgcagc
     1441 gcgcgtccga cagccttgaa gcaggcttcg accatgtggt gagcattctc gcccagcaca
     1501 gccatatgca gggacgctcc gagggactcc gcgaacgagc gaaagaaatg cggcacgagt
     1561 tctgtcggca gcccgcccac gctttcccgg ctgaactgtc cttcgaagac gaaccacggg
     1621 cggcccgaaa gatccagcga gaccctcgct tcgctctcat ccatcggcag caggaatccg
     1681 tagcgctcaa taccgcgctt gtctcccagc gctttgcgaa gggcttcgcc gaggcaaatc
     1741 gcggtgtcct ctaccgtgtg atgttcatcg acatccagat cgccgccgca ttcgaggcgc
     1801 atgctgaagc cgccgtgcct tgccacttgc tccagcatgt gatcgtagaa gccgatgccg
     1861 gtatcgacat gaatgggccc ggatgcgtca agatttacgg tcacgcggat ccgcgtctcc
     1921 cgggtttcgc gtgcgacagt ggcgagccgg gtggtggcct tcagtgtttg cgcaatgccg
     1981 caccagctga actcgaagcc gtcagtcggc ctgacgagca gcccccggat cccgatgttc
     2041 ttcgcaagtt gcaggtcggt gtcccgatcg ccgacgacgg cggagcgctc ccggtccagc
     2101 accgattcgg ccaggtatcg cgtcagcagc cctgcgttcg gtttgcgaca gccgcagccg
     2161 tcggctgagc ggtgggggca gataaagatc tcgtcgaacg tcacgccctg gctctcgaag
     2221 atggaaatta cgtgcctgtg gcagagctcg aagtcctgct tggggaacga ttcggtcccg
     2281 agaccgtcct ggttcgtcac catcacgaag cgaaagccgg catcccgaag actgatcaat
     2341 gccggcacga catcccggac aaggcgtatc ttggccagcg cgtcgacctg gttatccgcc
     2401 ggttcctcga tcagggttcc gtcccggtct atgaacagca cttttttcat atcagcttcg
     2461 ttccgcacag gtgccaagcg cctcgagaag caagttattc tcccacggct tgcctacgct
     2521 gatgcgtgca cagtttccca acgcagcgtc gtgaccgaac tcccggacca ggacgcggtg
     2581 tgcccgcagg cgctcctccg cctccggaaa gttcttgaac cttaccagca ggaaattact
     2641 gcgactcggc cagaccttca cgacgtccgg acattgtcgt aacgcaaccg ccagtcgatc
     2701 acgctcaccc agcaccttgc gggccgccag gcg
//
LOCUS       DASUOM010000364         8909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_743298,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000364 DASUOM010000000
VERSION     DASUOM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743298"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VFY27_07625"
     CDS             <1..1259
                     /locus_tag="VFY27_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765422.1"
                     /translation="AAAVGGGAALAVRRAAILEPATAMTPPPPPDYSRAVGSRVTSLK
                     FLDQQTRMILRQIVRFPSRAAFTAAGVAVSGSLLIGTLFFIDAMDEMIEVYFNVANRH
                     DVQVSFVEPRSRSAFFEVLRAPGVLDAEPYRSASARLRHEHLEERAALTGLVLDARLS
                     RMVDTVERAVVPPPGGLALSRDLAEKLQAHAGDLLQVEITEGKRPVVAVPIASVTTTY
                     IGSGAHMRIEDLNSLLQEGAVISGAYLTVDPALTDDLYVTLKEAPAVAGVNLQSLAQR
                     NFTEIMDQNIGMSIWIYTAFAGLIAIGVVYNSVRISFGERERELASLRVLGFSRGEVS
                     YILLGEIAFLTLLALPVGAGLGVLLALFLAQAMSSDLFRLPFVIDASTFGYAAIVVIF
                     VTAASGLLVRRQLDKMDLVSVLKSRD"
     gene            1275..2477
                     /locus_tag="VFY27_07630"
     CDS             1275..2477
                     /locus_tag="VFY27_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5765423.1"
                     /translation="MQLNFRSLFWIAAALLVLVLLVLAFRPQPVAADFAEVIQGPMQV
                     TVRDEGRTRVRDEYVVSAPVGGRLLRVDYKAGAEMQAGDTVARIVPGAPSFLDARAHA
                     EARAGVQSAAAALAAAQSELERAEAQERFARTELERVTEMHARELIAVETYDRARLQL
                     RVAESALSSARESVRMREADLDAARIRLTQPGGQGSDTAIVDVVAPVSGRVLRVAKES
                     ESVIAAGAEIISLGDPDNLEVVVEMLSTDAVLVRPGAEVIIQNWGRENDPLRGRVRLV
                     EPYGFLKISALGVEEQRVNVIVDFTQSREEWSSLGHGYRVEAAIVTWEADDVVQAPVA
                     SLFRSGGQWAVFRVEDDTAHLAPVEVGRDNGRTAQILSGLSAGDTVVLYPGETLSDGT
                     GVQRRGTE"
     gene            complement(2551..2775)
                     /locus_tag="VFY27_07635"
     CDS             complement(2551..2775)
                     /locus_tag="VFY27_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5765424.1"
                     /translation="MALTREEFEELTESFDYNDSDKDGMIEFTEFLNMLDALEAGIAP
                     TEARLGFDEIDSDDDGSIDLDEFVEWWSDR"
     gene            complement(2879..3220)
                     /locus_tag="VFY27_07640"
     CDS             complement(2879..3220)
                     /locus_tag="VFY27_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765425.1"
                     /translation="MADRGDTSLVVSALWMLVISMLLFFLPALNGLIGGAVGGFKAGS
                     VKRGLIAALLPAIAAGLGIWALLAVFDAPIIGFLSGLAFGVWALISSIGLLVGAMVGG
                     AMAPPRPAEIR"
     gene            complement(3309..3845)
                     /locus_tag="VFY27_07645"
     CDS             complement(3309..3845)
                     /locus_tag="VFY27_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765426.1"
                     /translation="MKGNLSLMQVTTLATERLTLRPLTFEDDAFILRLLNEPSFIRHI
                     GDKQVRTLEDARSYIGSGPQASHSTHGFGLDRVELSSTAQPIGMCGLLKRDILPDPDV
                     GYAFLPEFWSRGYASEAVCAVLNDARERLALKRVTAVVSNDNAASVRLLERMGFRFQR
                     MFEFHEGEPEVRLYRIDL"
     gene            complement(3962..5260)
                     /locus_tag="VFY27_07650"
     CDS             complement(3962..5260)
                     /locus_tag="VFY27_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX5765427.1"
                     /translation="MADEKKRFLFVSIGALISDIAWQVVKEGHDVRYFIESESEKSIG
                     DGFVPKVDAWEPHIDWADVIVFDDVLGMGEKAQELRAAGKHVVGGTAYTDKLEDDRSF
                     GQEELKRHGVSIIPFQDFSDFDSAIAYVEKNPSAYVIKPSGEAQNIKRLLFVGREDDG
                     KDVIHVLQSYKAVYADTVKVFQLQRRVRGVEVAVGAFFNGKEFMQPINVNFEHKLLFP
                     GDIGPSTGEMGTCMYWSGPNRIFKATLAKLEERLAEEGYVGYIDVNCIVNGQGIYPLE
                     FTARFGYPTISIQADSLSMPLADFLAGMADGTLKTFKFKRGFQIGVRIVVPPFPYNDP
                     KTFEANSKDRVVIFRRPNYEGIHIEDVKLVNGEWLITGNTGVVLIVTGQGSSVKQAQA
                     QCYQRVSNILIPNMYYRKDIGDRWFEDHDKLHTWGYLRES"
     gene            complement(5513..7276)
                     /locus_tag="VFY27_07655"
     CDS             complement(5513..7276)
                     /locus_tag="VFY27_07655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5765428.1"
                     /translation="MMTPSVRLVLVSLALLAACGEEAPQEPAANSGEAADRETADTGA
                     SPVADMVLRNGSIYTVDGTRSWANAVAIDDGRIVYVGSDEGADDFAGASTQVVDLRGR
                     MLLPGFQDVHIHPIGGGIEAAACDLNGLTTLDQYLEKIRTYAAENPDEEWILGGGWSM
                     SVFGPGANASRKLIDEIVPDRPVFLSSADGHSAWVNSKALEVAGITAETPDPADGIID
                     REPGSREPMGSLQEGAVSLVRAHAPPVDLETRMSGLQYAIEMLNSYGITSIQDASVDE
                     EDLKAYRALDDRGELSLRVVGSQWWARDKGTEQIPNFKILREQYTKGRVRAATVKIMQ
                     DGVMENYTAAMLEPYIGKGDMRGIPMVQPEALKNAVTALDAEGFQVHFHAIGDAAIRQ
                     SLDAVEAALKANGNLGHRHHISHLQLINEADVPRFRELDVVANFQPLWAFADSYITDL
                     TIPFIGPERTQWLYPIESVHRSGGMIAFGSDWSVSTANPFHEIETAVTRMGSDGETTE
                     PFLPEQRIDLPLALAAFTINAAYVNTHERDTGSVEAGKYADLIVLDRNLFDIDPREIS
                     ETSVLLTMVEGQVVHGDVASL"
     gene            7390..>8909
                     /locus_tag="VFY27_07660"
     CDS             7390..>8909
                     /locus_tag="VFY27_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765429.1"
                     /translation="MAKLSSSKGRFDEILELVGRGEYGHAESLCRALLQKYPDDVNVL
                     GLRGAVLIKLNRLEEAEQALRQAIRVAPAFAKPHEDLGLVLIGLRRPGEAADVLRNAV
                     RLDPQLELAWFNLGKALAMLGFGREADEAYEQSFRLNPERQKLAHAAEHHKEGRLEQA
                     EKLYREVVRDNPSNVDALRMLGRIALSAGRNADAERLFRRAVKLAPDFAGAIADLGRV
                     LKEQHQLQDAVECFERVIALEPENSQAHFQLAAALAPAAMTPRAIESYQKALALQPKF
                     PGAWLGLGHVLKTVGRQEEAIDAYRKCIRMRPDNGESYWSLANLKTYKLTDDDIAAMQ
                     QALEKEGLTNQSRVNFLYALAKAYEDRGNFEQAWKYYAQGNGTQRMIEKYDPVQTEVL
                     HDAIIDVFSAGFLADRPAAGHPDPSPIFVIGLPRSGSTLIEQILASHSQVEGTHELSD
                     LSKVVRGYRSRRKDRFPESLSGFGPGAWSRIGNEYLARTEKHRSGAPFFIDKNPNN"
BASE COUNT         1600 a         2776 c         2813 g         1720 t
ORIGIN      
        1 ccgcggccgc ggtcggcggc ggcgccgccc ttgccgtgcg ccgcgctgcc atcctggaac
       61 cggcgaccgc catgacgccg ccgccaccgc cggactattc cagggcggtt ggttcgcgtg
      121 ttaccagcct caagttcctc gaccagcaga cacgcatgat cctgcgccag atcgtgcgct
      181 ttccttcacg cgcggcgttc accgccgcgg gcgtcgcggt atccggttcg ttgctcatcg
      241 gcaccctgtt cttcatagac gcgatggacg aaatgatcga ggtgtatttc aacgtcgcca
      301 atcgccacga cgtgcaagtc agtttcgtcg aaccgcgctc gcgctctgct ttttttgagg
      361 ttttgcgtgc accgggagtt ctcgatgcgg aaccgtatcg ttccgcgtcg gcgcgcctcc
      421 ggcacgaaca cctggaagag cgggcagctt tgaccggtct cgttctcgac gcccggcttt
      481 cacgcatggt ggatacagtt gagcgcgcag ttgtgccgcc gccgggcggc ctggcgctgt
      541 ccagggacct cgccgaaaag ctgcaggcgc acgcaggcga cctgctgcag gtcgaaatca
      601 cggaggggaa gcggccggtc gtcgccgtac cgatagcctc ggttaccacc acctacattg
      661 gctcgggcgc gcacatgcgc atcgaggatt tgaacagcct gttgcaggaa ggtgcggtca
      721 tctccggcgc gtacctcacg gtggacccgg ccttgacgga cgacctctac gtgacattga
      781 aggaggcacc ggcggtcgcg ggtgttaacc tgcaaagcct ggcgcaacgc aacttcaccg
      841 agatcatgga ccagaacatc ggtatgtcga tatggattta cacagcgttc gccggcctca
      901 tagccatcgg cgtggtctac aacagcgtgc gaatttcgtt cggtgagcgg gaacgcgaac
      961 ttgcgtcgct gcgtgtgctc ggcttttcgc gaggcgaggt ctcttacatc ctgctcggcg
     1021 agattgcctt tcttactttg ctcgcactgc cggtgggtgc cgggcttggt gtgctgcttg
     1081 cgctgttcct ggcacaggcg atgagttccg atctcttccg gctgcctttc gtgatcgacg
     1141 cgtcgacgtt cggctatgcc gcgatcgtcg taatcttcgt gaccgccgcg tccgggcttc
     1201 tggttcgccg gcagctggac aaaatggatc tcgtctccgt gctgaaaagc cgggactagg
     1261 cgggcacttc aggcatgcag ctcaacttcc gttcgttatt ctggatagcg gctgctctgc
     1321 tcgtcctggt gttgctcgtt cttgccttcc ggccgcagcc cgttgccgca gacttcgcgg
     1381 aggtgatcca gggcccgatg caggtgacgg tgcgagacga aggtcgaacc cgcgtgcgcg
     1441 atgagtacgt tgtctccgct ccggtaggcg gccgtttgct gcgcgtcgac tacaaggcgg
     1501 gtgcagagat gcaggcgggt gacacggtcg cgcggatagt tccgggcgcg ccgtcctttc
     1561 tcgatgcgcg tgctcacgcc gaggctcgtg ccggcgtcca gtctgccgcc gcggcgctgg
     1621 ctgccgcaca gtcggagctg gaacgggcgg aagcgcagga gcgctttgca cgcaccgagc
     1681 tggaaagggt taccgagatg catgcgcgtg agcttatcgc cgtcgaaacc tacgaccgcg
     1741 cgcgcctcca gttgcgtgtc gccgagagcg cgctctcgtc ggcgcgcgaa agcgtccgca
     1801 tgcgcgaagc cgatctcgac gcggcgcgca tcaggctgac gcagccggga gggcagggca
     1861 gcgataccgc gatcgtggat gtcgtagcgc cggtgtccgg ccgcgtgctg cgggttgcaa
     1921 aggaaagcga gagcgtgatc gccgcgggcg cggagatcat ttcactcggg gacccggaca
     1981 acctcgaagt ggtggtcgag atgctgtcga cggatgcggt gctggtgagg ccgggagcgg
     2041 aagtcatcat ccagaactgg gggcgggaaa acgatccctt gcgcgggcgg gtgcggctcg
     2101 tcgaacccta cgggttcctg aagatttccg ccctgggcgt cgaggaacaa cgcgtgaacg
     2161 tcatcgtgga tttcacccag tcccgcgagg aatggtcttc gctcggccac ggctaccgtg
     2221 tcgaagctgc aatcgttacg tgggaagcgg atgacgtcgt gcaggcgccg gtagcgtcac
     2281 tgtttcgcag cggcggccag tgggcagtat tccgcgtgga ggacgataca gctcacctgg
     2341 ctccggtcga ggttggccgc gacaacgggc gaacggcaca aatcctgtcg gggctcagcg
     2401 ccggggatac cgtggtgctg tatcccggcg agaccttgag cgacggcacc ggggtgcaac
     2461 gacgcgggac ggagtaatcc tggagctcgc aggagctcgc catgcgagcg agttcgccaa
     2521 cgctgatcgc actcgcccgc gcggcgggcc ctagcggtcg ctccaccact cgacgaactc
     2581 gtcgaggtcg atggagccgt catcgtcgct gtcgatttca tcgaagccga ggcgggcctc
     2641 ggtcggggcg atgcccgctt ccagggcatc cagcatgttc aggaactcgg tgaactcgat
     2701 catcccgtcc ttgtcggaat cgttgtagtc aaagctttcc gtgagttcct cgaactcttc
     2761 gcgcgtcaac gccatcgggt aaccttcaag taaacataaa gcgggcgatc ataccgttgc
     2821 acgccgcccg ttggccaaca gcggcgccgg ctgttcatgg gcatcgtttt cgccactgct
     2881 aacgtatttc ggccgggcgg ggcggggcca tcgctccgcc aaccatcgcg cctacgagca
     2941 ggccgatact ggaaatcagc gcccagacgc cgaaagcgag cccggagaga aagccgatga
     3001 tcggtgcgtc gaaaacggcc agcagagccc agatgccaag acctgcggcg attgccggca
     3061 gcaacgcggc aatgagtccg cgcttgaccg atccggcctt gaagcctccg acggcgccgc
     3121 cgatgaggcc gttcaaagcc ggcaggaaaa acagcagcat gctgatgacc agcatccaca
     3181 gggcggaaac gaccagcgat gtatctcctc gatctgccat agcgaactcc tttgcgtgaa
     3241 gttccccccg ttcgaggatt atacagcggc ccatcctgtc ttgttccgtg cagaacgaga
     3301 gcgcggcgtt acaggtcgat ccggtagaga cgtacttcag gctcaccctc atggaactcg
     3361 aacatgcgct ggaagcgaaa gcccatccgc tcgagcagcc ggacggatgc ggcgttgtcg
     3421 ttgctgacca ccgcggtgac tcgcttcagc gcgagtctct cccgcgcatc gttcagtacg
     3481 gcgcacacag cctcgctcgc gtagccgcgg gaccagaact ccgggagaaa ggcgtagccg
     3541 acgtccgggt ccggcaggat gtcgcgcttc aacaatccgc acatgcctat cggttgtgca
     3601 gtcgaactca gttcgacgcg atcgagcccg aagccgtgcg tggaatggct ggcctgaggg
     3661 ccagacccga tgtaggatct ggcgtcctcg agtgtcctta cctgcttgtc gccgatgtgc
     3721 ctgatgaacg acggctcgtt gagaagtctg aggatgaagg cgtcatcctc gaacgtcagt
     3781 ggccgaagcg tcagccgctc cgtcgccagt gtcgttacct gcataagcga aagatttcct
     3841 ttcatccggt ggtttccagc atgccgtagc gctggtgcct ggccggaagc cgcacgggca
     3901 tcaaagcttc atcgggtcct gtatcaggga gaggcattcg gcactcgcgc caccgggtga
     3961 ctcagctttc gcgtaaatat ccccaggtgt gcaatttgtc atgatcctcg aaccagcgat
     4021 cgccgatgtc cttgcgatag tacatgttgg gtatgaggat attgctgacg cgctggtaac
     4081 actgcgcctg ggcctgcttc acggagctgc cctggccggt gacgatcagc accacaccag
     4141 tgttgccggt gatcagccat tcgccgttca ccagcttgac gtcttcgatg tgaatgcctt
     4201 cgtagttcgg gcggcggaag atcacgaccc ggtccttcga atttgcctcg aacgttttcg
     4261 ggtcgttgta cgggaacggc ggcacgacga tgcgcacacc gatctggaag ccgcgcttga
     4321 acttgaatgt cttgagcgtt ccatcggcca tgcctgcgag gaagtcggca agcggcatcg
     4381 acaggctgtc agcctggatg ctgatcgtcg ggtagccgaa gcgcgcggtg aactctagcg
     4441 gatagatgcc ttgcccgttg acgatgcagt tgacgtcgat gtagccgacg tagccttcct
     4501 ccgccagccg ctcctccagt ttcgccagcg tggccttgaa aatacgattg ggcccgctcc
     4561 agtacatgca ggtgcccatc tcgccggtcg agggcccgat gtcgccggga aagagcagct
     4621 tgtgttcgaa gttgacgttg atcggctgca tgaactcctt gccgttgaag aatgcgccga
     4681 ccgctacctc cacgccgcgc acccggcgct gcagctggaa taccttgacg gtatcggcat
     4741 agaccgcctt gtaggactgc agtacgtgga tgacgtcctt gccgtcgtcc tcccttccca
     4801 cgaacagcag ccgcttgatg ttctgcgcct cgccactcgg tttgatgacg taggcggaag
     4861 gatttttctc cacgtatgca atcgcggaat cgaagtcgct gaagtcctgg aaaggaatga
     4921 tggaaacgcc gtgccgcttc agttcttcct ggccgaaaga acgatcgtcc tcgagcttgt
     4981 cggtgtaggc ggtgccgcct acgacatgct tgcccgcggc tcgaagctcc tgcgccttct
     5041 cgcccatccc aaggacgtcg tcgaagacga tgacgtcagc ccagtctata tgcggttccc
     5101 acgcatccac cttcggcacg aagccgtcgc cgatgctctt ctcggattcg ctttcgatga
     5161 agtacctgac gtcatggcct tccttcacga cctgccaggc gatatcgctg atcagcgccc
     5221 cgatcgagac aaagaggaag cgcttttttt cgtcagccat gcaccgggcc atgaagggag
     5281 gaagggacgt ccggttgcat tatgcatggc ggcaaaagca ttagaaaccg tgtgagcatc
     5341 ctggaggcgc tgtgggagcc cacgttaagc ggaagcgcga cgaatccgat ccgtatccgc
     5401 cttgatggac ggcgactgcg ggcgagttcg cctaagcgga atcacactcg cccgcatggc
     5461 gggctcctac gatacgaatc ccgttcgtca caggttcctt cgccgtgggc gcctacaacg
     5521 acgccacgtc cccatgcact acctggccct cgaccattgt cagcaggacg ctcgtctccg
     5581 agatttcccg cgggtcgatg tcgaacaggt tccggtcgag cacgatgagg tcggcatact
     5641 tcccggcctc caccgagccc gtgtcccgtt catgcgtgtt cacgtaagcc gcgttgatgg
     5701 tgaatgcggc cagcgccagc ggcaggtcga tgcgctgctc cggcaggaac ggctctgtcg
     5761 tttccccatc ggagcccatg cgggtgacgg cggtttcgat ctcatgaaac ggattcgcag
     5821 tggatactga ccagtcgctg ccgaaggcga tcatgccgcc gcttcgatgc acgctctcga
     5881 tagggtaaag ccattgtgtg cgctcgggac cgatgaacgg aatcgtcagg tcggtgatgt
     5941 agctgtcggc gaacgcccac aggggctgga agtttgcgac cacgtcaagc tcgcgaaaac
     6001 gcggcacatc cgcctcgttg atcagctgca ggtgcgagat gtggtgccga tgccccaggt
     6061 tgccgttcgc tttcagcgcg gcttcgaccg cgtccagcga ctggcggatg gcggcgtcgc
     6121 cgattgcgtg gaagtgcacc tggaatcctt ctgcatcgag tgcggtcacg gcgttcttca
     6181 gcgcttccgg ctgcaccatc ggtatccccc gcatgtctcc cttgccgatg tatggctcca
     6241 gcatcgccgc cgtgtagttt tccatgacgc catcctgcat gatcttgacg gtcgccgcgc
     6301 ggacgcggcc tttcgtgtac tgctcgcgca ggatcttgaa gttcgggatc tgctccgtgc
     6361 ccttgtcgcg cgcccaccat tgggacccga caactctcag gctcagctcg cctcgatcgt
     6421 ccagcgcccg gtaggctttc agatcctctt cgtcgacact cgcgtcctgg atcgaggtaa
     6481 tgccgtaact gttcagcatc tcgatcgcgt actgcaggcc ggacatgcgc gtctccaggt
     6541 cgaccggcgg cgcgtgcgcg cgcacgagcg acacagcccc ctcctgcagg ctgcccatgg
     6601 gttcccggct gccgggctcg cggtcgatga tgccatctgc cggatccggg gtttcggcgg
     6661 tgatgccggc gacttccaac gccttgctgt tgacccatgc cgagtggccg tctgccgagc
     6721 tcaggaatac ggggcgatcc ggtacgattt cgtcgatgag cttgcggctc gcgttggcgc
     6781 cgggaccgaa gacggacatc gaccagccgc cgcccaggat ccactcctcg tcggggtttt
     6841 cggccgcata cgtccgtatc ttctcgagat actggtcgag cgtcgtcaga ccgttgaggt
     6901 cgcaggctgc ggcttcgatc ccgccgccta tcgggtgtat gtgcacgtcc tggaagccgg
     6961 gcagcagcat gcgcccgcga agatcgacga cctgcgtcga agcaccggcg aaatcatcgg
     7021 cgccctcgtc cgacccgacg tacacgatcc ggccgtcatc tatggcgacc gcattcgccc
     7081 aggaacgcgt tccatccacc gtatagatgc tgccgttccg cagcaccatg tccgcaaccg
     7141 gtgaagcgcc cgtgtccgcg gtctcccggt ccgctgcctc gccggaattc gcagcaggtt
     7201 cctgcggggc ttcttcgccg cacgcagcga ggagggccaa actcacgagt accaggcgaa
     7261 ccgatggtgt catcattatt ctgttccttc cccgatgcga tatagatgcg aaatcactac
     7321 ttgttgcacg gcattctatg ccgcggcaca gtgtttcaga aatcgctcat agctcatact
     7381 cgatcccaca tggcgaaact ctcatcctca aaaggccggt tcgacgagat tctcgagctg
     7441 gtcggccgcg gcgaatacgg ccatgcagag agcctctgcc gggccttgct gcagaaatac
     7501 ccggacgacg tcaacgttct cggacttcgg ggcgccgtgc tgatcaagct gaaccggctc
     7561 gaagaggcgg agcaggcgtt gcgccaggcg atccgcgtcg cacctgcgtt cgccaaacct
     7621 cacgaggact tagggctggt cctgatcggg ctcaggcggc cgggagaggc tgccgacgta
     7681 ctgcggaacg cggtgcggct ggacccgcag ctcgagcttg cgtggttcaa cctgggaaag
     7741 gcgcttgcca tgctcgggtt cggcagggag gcggacgaag cgtacgagca gtctttcagg
     7801 ctgaacccgg aacggcaaaa actggcccat gccgcggagc atcacaagga agggcgcctg
     7861 gaacaggcgg aaaagcttta tcgcgaagtc gtgcgggaca atccatcgaa cgtcgatgcc
     7921 ttgcgcatgc tcggccggat cgctctcagc gcgggacgca atgccgacgc cgagcggctg
     7981 ttccgccgcg ccgtcaagct ggcgccggat tttgccggcg ccatcgccga tctggggcgc
     8041 gtgctcaagg aacagcacca gctgcaggac gcggtcgagt gtttcgagcg cgtcattgca
     8101 ctggagccgg aaaattccca ggcgcatttc cagctcgccg cggctctcgc gccggcggca
     8161 atgacgccgc gggccatcga gtcgtaccag aaggcgcttg cgctgcagcc gaagtttccc
     8221 ggtgcgtggc tggggctcgg ccacgtgctg aagaccgtcg gccggcaaga ggaagcgatc
     8281 gatgcctatc gcaagtgcat acgcatgcgc cccgacaacg gggaaagcta ctggagcctt
     8341 gcaaacctga agacctacaa gctgacggac gacgatatcg ccgccatgca acaggcgctg
     8401 gagaaggagg gactgacgaa ccagtcacgg gtgaacttcc tgtatgcgct tgcgaaggca
     8461 tacgaagacc gcggcaactt cgagcaggca tggaagtatt acgcgcaagg caacggaacg
     8521 cagcgcatga tcgagaagta cgatccggtg cagacggaag tgctgcacga cgcgatcatt
     8581 gatgtgttca gcgccgggtt tcttgccgac cgtcccgcgg ccgggcaccc ggacccctcg
     8641 cccatcttcg tcatcggcct gccgcgctcg ggctcgacgc tgatcgagca gattctcgcg
     8701 tcgcacagcc aggtggaggg cacacacgag ctgagcgatc tctcgaaagt cgtacgcggt
     8761 tatcgcagcc gccggaaaga ccggttcccg gagtcgttga gcggcttcgg acccggcgcc
     8821 tggtcacgga tcggcaacga atacctggcg cggacggaga agcaccgcag cggcgcgccg
     8881 ttcttcatcg acaagaaccc gaacaactt
//
LOCUS       DASUOM010000365         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1804786,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000365 DASUOM010000000
VERSION     DASUOM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804786"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFY27_07665"
     CDS             <1..352
                     /locus_tag="VFY27_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5765430.1"
                     /translation="ARVTQVGRFIRQYRLDELPQLFNILRGNMSLIGPRPERPEFVQD
                     LQKVIPYYSVRHTVKPGLTGWAQLRYTYGATEEDALEKLQYELYYIKNHSLLLDILIL
                     LQTVEVVLWGKGAR"
     gene            complement(373..1470)
                     /gene="wecB"
                     /locus_tag="VFY27_07670"
     CDS             complement(373..1470)
                     /gene="wecB"
                     /locus_tag="VFY27_07670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX5765431.1"
                     /translation="MQTIHLIAAARPNFMKIAPLFHLLATTDWAKPVLVHTGQHYDRN
                     MSDAILEDLGVPAPDFHLGVGSGSHAEQTAKVMIEYEKICEMYRPDWIIVVGDVNSTV
                     ACAMVGAKLWIPVIHLEAGLRSRDRRMPEEINRLVTDAIADVLWTPSADADENLLAEG
                     IPPSRIDRVGNIMIDSFEMLRDQIMASNARESHRLENGKYALVTLHRPSNVDTVEALA
                     SIVDVLADAARDLPVVFVAHPRTMNSIERFGLRQKLESASGLLLLPPEPYIRFMNLVT
                     GAKVVITDSGGLQEETTYLSIPCLTLRENTERPVTVSLGTNRLATPESLPGQLTEILE
                     GRWPKGQCPDLWDGRTAERAVACLERRLRLE"
     gene            1823..>3686
                     /gene="prsT"
                     /locus_tag="VFY27_07675"
     CDS             1823..>3686
                     /gene="prsT"
                     /locus_tag="VFY27_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HEX5765432.1"
                     /translation="MRVRNVLILCLLSMVGCGLATSNEERLDRAEAAFEEGAYRAAMI
                     DARDVLRREPDNVRGRLLLGRVSLAVGDVAAAEKELRRAVDLGTPLEHVILDLGISVL
                     RQGKYQDLLDEFPAEPAITAAEQVQWMLVRADALLGLGQIESARDVYSAAISSEPDNV
                     LAKMGVVATYQAEQNLLQARITLDEVLAAHPEAPEPWLVSGEIDLRLQRYEEAATSFG
                     RALELLDSAGSSATAARALAGLAAAQLSLQHTDAARETVSRLEALAPGSVSALQLSAS
                     LAYGDEDLDAAQQKLQLILQQAPDNTAAQMLLGVVHLESGNLAQAEMYLSSVVAGAPE
                     NIEARTLLAQTRLQLQRFDQARETLAPMLSEHNPDSRMLAVAALLDIETGNVDRGVEY
                     LEQSVAASPNNAELKLQLAAAYVSAGRIAEAQALLDSVEPGAVDNDEYRRGLLQALVK
                     FHEGAVDEAIASGQETAHRFPGRSEANIMIGYFHLSRREFEAARKYFELGASQTGGRA
                     MAQRYLGAIDMAEGKLDTAEERYAAILKMQPHATWAMVAMAGIASEREDKVNTIRWLE
                     RARESDPADVASRALLARLYLGEQKFKEAETVSREAIAISPDNASLHNMLGVSLL"
BASE COUNT          780 a         1042 c         1139 g          725 t
ORIGIN      
        1 tgcccgggtg acgcaggttg gtcgatttat tcgtcaatac cgactagacg aactacctca
       61 gctgttcaac atccttcggg gcaacatgag cctgatcggc ccccggcccg agcggccgga
      121 gttcgtacag gatctgcaaa aagtcattcc ctactattcg gttcgacaca ccgtaaagcc
      181 cggccttacc ggctgggcac aacttcgcta tacctacgga gcgaccgagg aagacgcact
      241 ggaaaagctt caatacgagc tgtactacat caagaaccac agtttgctgc tggatatatt
      301 gatcctgctg caaacggtgg aagtcgtact gtggggtaag ggcgctcgct gaagcaagca
      361 gcggaatctg tctcattcca ggcgtaatct ccgttccagg cacgcaacgg cacgttccgc
      421 ggtgcgcccg tcccacaagt ccggacattg tcccttgggc catcggccct ccaggatttc
      481 cgtcagttgc ccgggcaggg actccggcgt ggccaggcga ttagtaccca gcgagaccgt
      541 aacgggtcgc tcggtgtttt cccgcaacgt aaggcacgga atggacaagt atgtcgtttc
      601 ttcctgcagt cctcccgaat ccgtgatgac gactttcgcg ccggtaacca ggttcatgaa
      661 gcgaatgtac ggttcaggcg gaagcagcag gaggccggag gcgctttcaa gcttctgtct
      721 gagaccgaag cgttcaatgc tgttcatggt gcgcggatgc gcgacgaata cgacagggag
      781 atctcgtgcc gcatcagcga gcacgtcgac tatcgacgca agggcttcca ccgtgtcgac
      841 attcgaaggg cggtgtaatg tgaccagggc atattttcca ttctccagcc gatgcgattc
      901 acgggcgtta cttgccatga tctggtctcg aagcatttcg aaggagtcga tcatgatatt
      961 gccgacccga tcgatccgcg atggaggaat gccttccgcc agcaggtttt cgtcggcatc
     1021 ggccgatgga gtccacagaa cgtccgctat cgcatcagtt accagccggt taatttcttc
     1081 cggcatccgc cggtcgcggc tgcgcagccc cgcttcgagg tggatgacgg gtatccacag
     1141 cttggcgccg accattgcgc aggccaccgt cgagttgaca tcgccgacaa cgataatcca
     1201 atccggtcgg tacatttcgc agatcttctc gtactcgatc atgaccttcg cggtctgctc
     1261 ggcatgggag ccgctgccga cgccaaggtg gaaatccggg gccgggacgc cgaggtcttc
     1321 caggatggca tctgacatat tccggtcgta gtgttgcccg gtgtgaacca ggacgggctt
     1381 cgcccagtcc gtcgtcgcca aaaggtgaaa aagtggcgcg attttcataa aattgggtcg
     1441 ggcggccgca atcaagtgga ttgtttgcaa ggggcgctcc gcaagaaaaa aactcgcgta
     1501 tagtagcgag ctgaatgagg gttgtctgcc cgttgctttt tccggagaca cagcgcgaac
     1561 ctgatcgacc ggtcggactg cttcggcgga cgaggggaac cgaccttaca gcagggcagg
     1621 caagcactag ccgcacgtaa tgcccgtgca ataaaactgc aaagtccagg ttcggggatt
     1681 aacacttcga gtgctccagt gcactctcag cctgcgaggt tcaacgagaa cctcagcgaa
     1741 aataagaatg ccggcctctg ctatcaatgg acacgttcga acgtatccgg ccccgcgccc
     1801 gatatgtcgt gagtggtact caatgagagt caggaatgtc ttgattctct gtcttttgtc
     1861 catggtcggc tgtggattgg cgaccagtaa cgaggagcgg ctggacaggg cggaagcggc
     1921 gttcgaagag ggcgcttatc gagccgctat gatcgatgca agagacgtgt tgcggcgcga
     1981 gccagacaat gttcgtggcc gactgttgct cggacgcgtt tctctggcgg tcggagacgt
     2041 cgccgccgca gagaaggaac tccgcagggc ggtggacctg ggcacgccac tcgagcacgt
     2101 catcctcgac ctgggaatta gtgtcctccg gcagggcaag taccaggacc tgctcgacga
     2161 atttcccgcc gagccagcaa taacagcagc cgaacaagtc caatggatgc tcgttcgtgc
     2221 cgatgcgctt ctcggtctcg gccagatcga gagcgcgagg gatgtctact ccgcagcaat
     2281 ttcgagtgaa cccgataacg tcctggcgaa gatgggtgtc gtcgcgacat atcaggcaga
     2341 acagaatctc ttgcaggcga ggatcacgct tgacgaagta ctggccgcac atccggaagc
     2401 cccggagccc tggttggttt cgggggagat cgaccttcgc ctgcagcgct acgaggaagc
     2461 ggcgacaagt ttcggcaggg cactcgagtt gttggatagc gcaggaagca gcgcgaccgc
     2521 tgcgcgcgcg ctggcgggtc ttgccgcagc gcagctttcg ctgcagcaca cggacgcagc
     2581 tcgggaaacg gtttcgcggc tcgaagctct ggcgccgggc tccgtgtctg cgctgcaact
     2641 ctccgctagc ctggcctacg gggacgagga cctggacgcg gcacagcaaa agctgcagct
     2701 aattctacag caagcaccgg acaacaccgc ggcgcagatg cttcttggcg ttgtgcatct
     2761 cgagagcggc aatcttgcgc aggcggaaat gtacctgtcg tctgtcgttg ccggtgcgcc
     2821 ggagaacatc gaggcgcgca cgctactggc acagacgcgt ctgcagctcc agcgcttcga
     2881 ccaggcccgc gagacgctgg cgccaatgct ctcggaacat aatcctgact cccggatgct
     2941 ggcggttgcg gctctgctgg acatcgagac cggaaatgtt gacaggggtg tcgagtatct
     3001 cgagcagagc gtagcagcca gcccaaataa cgcggaactc aagttgcagc tcgcagcggc
     3061 atatgtatcg gctggcagaa tcgcggaagc gcaggctctt ctcgattcgg tcgagccggg
     3121 cgcggtggac aatgacgagt accggcgcgg cctgttgcaa gcgctggtga aattccacga
     3181 gggggcggtc gacgaggcca ttgcgtcagg tcaggaaaca gctcatcggt ttccggggag
     3241 atccgaagcc aatatcatga ttggatactt tcacctgagc cggcgcgaat ttgaagcggc
     3301 caggaagtat ttcgaattgg gcgcatctca gaccggcggc cgggccatgg cgcaacgata
     3361 tcttggcgcc atcgacatgg ccgagggcaa gctcgataca gcggaagagc gctatgcggc
     3421 cattctgaag atgcaacccc atgcaacctg ggctatggtg gcgatggccg gtatcgcgag
     3481 cgaacgtgag gacaaagtca ataccattcg atggcttgaa cgagcgcgcg agagcgatcc
     3541 ggccgatgtg gcgtcacgtg cgctgctcgc aagactttac cttggcgagc agaaattcaa
     3601 ggaggccgag acggtttccc gggaggcgat cgcgattagt cccgacaatg catcattgca
     3661 caatatgctg ggcgtttccc tgctcc
//
LOCUS       DASUOM010000366         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_641308,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000366 DASUOM010000000
VERSION     DASUOM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641308"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..495)
                     /locus_tag="VFY27_07680"
     tRNA            complement(420..495)
                     /locus_tag="VFY27_07680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Thr,seq:tgt)
     gene            complement(572..1264)
                     /locus_tag="VFY27_07685"
     CDS             complement(572..1264)
                     /locus_tag="VFY27_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765433.1"
                     /translation="MKNLLLLLVLANILYFIWEMVIEEPPEAGVAVVEISRLGPPLQL
                     SQGSEAGPEAASSVGAMPGTSAQTDLAAVVGRSCVSIGPFTSGPDAQRVFQDYRKEDM
                     RASVRSTQGQIFVGHWVQIRDIPDRQTGNAMIDRLVAGGITDAYLVPTDDEGLKISLG
                     LFGEMSRAERVELQAEAMSMQAEITPRVREDTVFYVDIVLPPGRGAGTMIERFGEERV
                     LLRELATCPGAG"
     gene            complement(1261..2031)
                     /locus_tag="VFY27_07690"
     CDS             complement(1261..2031)
                     /locus_tag="VFY27_07690"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5765434.1"
                     /translation="MSSVRALLFDAGNTRLKWGLLAQGRIGKSGSITHEKLHDSGFGV
                     LTANLPRDVDTVLVSNVAGQTFATRLSGVIGLHCNCDVHFARAEREGYGVTNSYRRPR
                     RIGVDRWVAMVGAYAEFRSALCVVDAGTAVTIDAVDRSGTHLGGMIVPGLSMMAGALH
                     VNTREIPLAKRNLAAMADAMNAFANTTEGAVQNGALTAISGAIERSVRLLRAAGFRPK
                     VVLTGGDASRILKLLDANVLHRPNLVLEGLAFMVQSEK"
     gene            complement(2028..2858)
                     /locus_tag="VFY27_07695"
     CDS             complement(2028..2858)
                     /locus_tag="VFY27_07695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5765435.1"
                     /translation="MTIVTMTDPDDILQRIGSDARSSLELLEVFPELESTNSWLLQQR
                     SPSRGRFRAVLAEHQTAGRGRSDKIWLSPGGSGLCLSLAYTFAELPRHLPSLTLAIGA
                     AIATALENLGAKDLALKWPNDLMAQDSKLGGILTELKTNGKGSRAVVVGLGLNVDLPD
                     TMRYTPPTSWTSKITDLAACLPELPDRAALIAAVLNSIVDCIERFVREGFSHFHSIWQ
                     RYDWLKGKRWSVQQAQRRITGIAEGIDGHGALLLRTSAGLERVISGSIQLPSCGAASG
                     "
     gene            complement(2836..3522)
                     /gene="plsY"
                     /locus_tag="VFY27_07700"
     CDS             complement(2836..3522)
                     /gene="plsY"
                     /locus_tag="VFY27_07700"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5765436.1"
                     /translation="MLELGTKFLISYFVGSLMGSMIVGKLRGGVDIRSMGSGNAGGTN
                     ALRTQGWKFALGVLAIDIGKGAFAAGVVPSLNLPVVGTDPSISRVWLTLSCAAAAVIG
                     HVWPVWHNFRGGKGAATLIGTLIVLAPSLILPVLLVWAWILTLFGYVGLATMTAALFA
                     PLYLALTRLPGDQPLFIYSLVLSLFLLYCHRSNIRRMLAGTESRNTHLMIFRRKREPA
                     SANNDNSDND"
BASE COUNT          692 a         1182 c         1060 g          688 t
ORIGIN      
        1 gctcacattg attggcaatc gcgtgccggt aacgactatt tcctcgatga catcgtcacg
       61 gggcgtgggc gtttcggtct cggctgccag cgctgtcggc acaagcaacg catcgacgaa
      121 agacaacatc gtcgagcaac gagcgaaggc agggtgacgc ataaaccatc ctgccggagc
      181 ggtgcctatg gcaatgacta gccccgatcc cgtatgacct cggccaatcc gcgctcaggc
      241 aagagcgatc atagcacctt gtgaccggcc gttagtgcgc ttccaatagc cgcaaatgac
      301 ccgagggaac ggctggcaat tctgaacccg gtcgcaagat cggtgtggaa tgtcagcaca
      361 gtcgtcatgc ggagtacgag ccactattcg gcaaccaacc ggaattacat tagaattttt
      421 ggtgccggca ccaggagtcg aacccgggac ctactgatta caaatcagtt gctctaccaa
      481 ctgagctatg ccggcaatct gttgttcttc tgcgtttcag gctgggctgc gaattattcg
      541 ccactcgcag ccgaacgaca agtgcggcgg actatcccgc tccaggacag gtcgccagtt
      601 cccgtaacag caccctctcc tcgccgaacc tctcgatcat ggttcccgct cctcgccccg
      661 ggggcagcac gatatcgacg tagaaaacag tgtcctcgcg cacacgcggc gtgatctcgg
      721 cttgcatgct catggcctcc gcctgcagtt ccacgcgctc cgcgcggctc atctcgccga
      781 acaggccgag agatattttc aggccttcgt cgtccgtcgg taccaggtat gcgtcggtga
      841 ttccgccggc gaccagcctg tcgatcatcg cgttaccggt ctggcggtcc gggatgtcgc
      901 ggatctgcac ccaatggccg acgaagatct gtccctgcgt cgagcgcacg gaggcgcgca
      961 tgtcttcctt gcgataatcc tggaatacgc gttgggcgtc ggggccgctg gtgaatggtc
     1021 ctatgctgac gcaggatcgg ccgactacgg ccgccaggtc cgtttgcgcc gaggtgccgg
     1081 gcatcgcacc tacgctcgac gccgcttccg gtcccgcttc cgatccttgg gaaagctgca
     1141 gcggcgggcc cagcctggag atctcgacga cggcgacacc ggcttcaggc ggctcctcga
     1201 tcaccatctc ccagatgaaa taaaggatat tcgccaggac aagtagcagc agcaggtttt
     1261 tcatttctcg ctctgtacca tgaacgcaag gccctccaac acgagattgg gccggtgcag
     1321 cacatttgcg tccaggagct tcaggatgcg tgacgcatcg ccgccggtca agacgacctt
     1381 cggccgaaat cccgccgcgc gcaacaatcg aacggaccgt tcaatcgcgc cgctgattgc
     1441 cgtcagcgca ccgttctgca ccgcgccttc ggtcgtattg gcgaacgcgt tcatcgcatc
     1501 tgccattgcg gccaggttcc ttttggccag cgggatctcc ctggtgttca cgtgcagcgc
     1561 gcctgccatc atcgagaggc ccggcacgat catgccgccc aggtgcgttc cgctccgatc
     1621 cacggcgtcg atagtgactg cggtaccggc atcgaccacg cacaacgccg agcggaactc
     1681 cgcataagca ccgaccatcg ccacccaccg gtccacgccg atcctgcgcg gccgccggta
     1741 gctgttcgta accccgtatc cctcgcgttc cgcgcgcgcg aagtgcacgt cgcagttgca
     1801 gtgcaggccg atgacgcccg acaggcgcgt cgcgaacgtc tgcccggcaa cgttgctcac
     1861 caaaacagta tcgacgtctc tcggcagatt cgccgtcagc acgccgaaac ccgaatcgtg
     1921 cagcttctcg tgcgtgatgc tgccgctctt cccgatgcgt ccctgtgcca gcaagcccca
     1981 tttgaggcgc gtgtttcccg cgtcgaacag caacgcacgt accgaactca tccgctggcg
     2041 gccccgcagg atggaagctg gatggagccg gaaatgacgc gttccagccc ggcgctggtc
     2101 ctcaggagaa gcgcgccatg cccgtcaatc ccttccgcga taccagtaat gcgacgctgc
     2161 gcctgctgca cgctccaccg cttgcccttg agccagtcgt agcgctgcca tatggaatgg
     2221 aaatggctga aaccctcgcg cacgaagcgc tcgatacaat cgacaatgct gttgaggact
     2281 gctgcgatca gcgcggcgcg atcaggcagt tccggcaggc atgcggcgag atccgtaatc
     2341 ttgctggtcc acgaggttgg cggcgtgtag cgcatcgtat ccggcagatc cacattcagg
     2401 ccaagtccca ctaccaccgc gcgactgcct ttaccgttcg ttttaagctc cgtcaggatc
     2461 cctccgagct tactgtcctg cgccatgagg tcgttcggcc atttcagcgc caggtccttc
     2521 gcgccgaggt tctccaatgc cgttgcgatc gcagcgccaa ttgccagcgt caggctcggc
     2581 aaatgccgcg gcagttccgc gaacgtgtat gcgagcgaaa gacacaggcc cgatccgccc
     2641 ggcgacagcc agatcttgtc gctgcggccg cggcccgccg tctggtgttc ggcaagcacg
     2701 gcgcgaaacc gcccgcggct cgggcttctc tgttgcagca gccagctgtt ggtcgactcc
     2761 agttccggaa acacctccag caactcgagc gatgaacgcg catcgctgcc gattctttgc
     2821 aggatgtcgt cggggtcagt cattgtcact attgtcatta ttggcgcttg cgggttcccg
     2881 cttgcggcgg aagatcatga ggtgcgtgtt cctcgactcc gtgccggcca gcatgcgccg
     2941 gatgttggag cgatggcagt agagcaggaa cagcgacagc acgaggctgt atatgaagag
     3001 cggctggtca cccggcaggc gcgtcagcgc gagatacagc ggcgcgaaga gtgccgccgt
     3061 catggttgcc aggccgacgt agccgaacag cgtcaggatc cacgcccaca ccagcaacac
     3121 gggcaggatc aggctcggtg ccagaacgat cagcgtcccg atgagcgttg ccgcaccctt
     3181 gccgccgcga aaattgtgcc acaccggcca gacatgcccg atcaccgctg ccgcggcgca
     3241 acttagagtt agccacaccc gcgaaatcga gggatcagtg ccgacgacgg gcaggttcag
     3301 tgatgggacg actcctgcgg cgaacgcgcc cttgccgata tcgatcgcca gcacgccgag
     3361 cgcgaacttc cagccttgcg tgcgcaacgc gttggtgccg ccggcgttgc cgctgcccat
     3421 gcttcgtata tcgacgccgc cacggagctt cccgacgatc atcgaaccca tcagcgagcc
     3481 aacgaagtag ctgatcagga atttggtgcc gagttccagc atgaaaaaac tggcgcctgt
     3541 cccgtaccgg atgtcggccg gcattgtagg cgccttgcag aggctttgtc ttggttacgg
     3601 catcattgac atatatttag at
//
LOCUS       DASUOM010000367         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_566468,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000367 DASUOM010000000
VERSION     DASUOM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566468"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1199)
                     /locus_tag="VFY27_07705"
     CDS             complement(84..1199)
                     /locus_tag="VFY27_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765437.1"
                     /translation="MLQSQCDIGKGVPAKAARKIAVVALCLTAGAAASWAGKASPVPP
                     GAALPEKLSQTGLFLPGTTDVAADLLPFAPQYPLWTDGATKRRWLHLPPGTSIDAAKP
                     DAWVFPAGTRVWKEFGYDGPVETRMIERLADGSWRYATYIWNEDGSDAELAPTEGVPA
                     LPVSGAPDGIYSIPSENDCRGCHEAAAQPVLGFSALQLSPDRDPLAPHAERPDRAYVD
                     LRVLHERGLLLQLPEELVKTPPRIAAPSPQARAALGYLHGNCGHCHNDTGPIAELDLS
                     LRQSAVARSASAARTLESLVGYPAEVSTKKIDTRVVPGSHESSMLFARMRTRNPLMQM
                     PPLGTRVSDATAVALIGSWIEQDLSNPSETLARGHHQ"
     gene            1375..2325
                     /locus_tag="VFY27_07710"
     CDS             1375..2325
                     /locus_tag="VFY27_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5765438.1"
                     /translation="MDQLSCMRIFVRVVEQGALSRAADDLAISRTSVTNAISQLEKKL
                     GVRLLNRTTRRLSLTEEGSSYYTNCVRILGEIAEAEDSLTSAKTSPRGCLRVSIPQSF
                     EALSFFPLLHEFMEQHPALRVEVIVTDRAVNLVEEGMDCALRAVEIPPDSALVARRLF
                     RSTWLTCASPAYLAQNGTPRCISDLSEHNCIRFVSPSSCRVRDWTFDVDGGITCVTPA
                     GNLSVTSLDGAVVAARTGAGIAQVPDALAFRAIMAGELRPILTRHIAESVPLMLTYPG
                     NRYLPAKVRAFGKFFENAYPKAGLWPQIAAKLEAEGRTAA"
BASE COUNT          420 a          774 c          803 g          482 t
ORIGIN      
        1 ggccaaagct gcgcgggggc ccgcaatgcc aagtgcaccc atcatcgcca gcgccagggc
       61 gcgagacgcg ttgttgtagt tgctcattga tgatgtcctc tcgctaatgt ttcactcggg
      121 tttgaaaggt cttgttctat ccaggatccg atcagggcca ctgcggtcgc gtcgctgacc
      181 cgggtaccaa gcggtggcat ctgcatcaac ggattgcgtg ttcgcatcct ggcgaacaac
      241 atgctggact cgtgtgaacc cggaacgacc cgggtatcga tttttttcgt gctgacctct
      301 gcggggtagc cgaccaggct ttcgagggtc cgcgccgcgc tcgcggagcg ggctactgct
      361 gattgccgca aagacaggtc cagctctgct ataggtccgg tgtcgttgtg gcaatgcccg
      421 cagttgccgt gcaggtaacc gagcgccgct cgagcctgcg gggagggcgc tgcgatgcgc
      481 ggcggcgtct tgaccagttc ctccggcagt tgcaacagca gcccgcgttc atgcaggacg
      541 cgcaggtcca cgtatgcccg atccggcctt tcggcgtgcg gtgccagcgg atcccggtca
      601 ggcgaaagct ggagcgcgct gaacccgagc acaggttgag ccgctgcttc atgacagccg
      661 cgacagtcgt tctccgaagg tatggagtaa atgccgtccg gagcgccgga taccgggagg
      721 gccggaacac cttctgtggg cgccaactcg gcgtcgctgc cgtcttcgtt ccagatgtag
      781 gttgcgtagc gccaggagcc gtccgcgagc cgctcgatca tccgcgtctc aaccgggccg
      841 tcatagccga actctttcca tacgcgggtg ccggcgggga agacccaggc gtccggcttc
      901 gccgcgtcga tgctcgttcc cggcgggaga tgcagccacc gccgcttcgt ggccccgtcc
      961 gtccagaggg ggtactgcgg cgcgaacggc aggaggtccg cggcgacgtc ggtggtgccg
     1021 ggcaggaaca ggccggtctg gctgagtttt tccggcagcg ctgcaccggg aggaacggga
     1081 gaggccttgc ctgcccacga ggcagcggca ccagccgtca ggcacagcgc gaccacggcg
     1141 atcttccgcg ccgctttcgc gggcacgccc ttgccgatat cgcactggga ctgcaacatg
     1201 ctctgcttcc tgggtctgtt tgaggttcgt cggttccggc cgttcttaca gggccttccg
     1261 tggcgctcca gggcgtattg acgaaccgca tcggggggcg cactgtattc gcgtcgcgga
     1321 aaccgataaa ccctgtcccg gcaaattgat tgttcgggaa ggcggaataa tcgtatggac
     1381 cagctctcgt gcatgcgcat ctttgttcgt gtcgtcgagc agggagcgct ttcacgggcc
     1441 gccgacgatc tcgccatttc ccgaacttcc gtcaccaacg ccatctcgca gctggagaag
     1501 aagctgggag tccggctgct gaaccgcact acccggcgct tgagtctgac ggaagaaggc
     1561 agctcttatt acacgaactg tgtccgcatc ctcggggaga tagcggaggc ggaggacagc
     1621 ctgacgagcg caaaaacttc cccgcgcggt tgccttcgcg tctccatccc gcagtctttc
     1681 gaagcactgt cgttcttccc gttgttgcac gagttcatgg aacagcaccc tgcgcttcgc
     1741 gtggaagtca tcgttaccga ccgcgcggtc aacctggtgg aagaaggcat ggactgtgca
     1801 ctgcgggcgg tagaaattcc gccggactct gccctggtgg cgcgcaggct ctttcgttct
     1861 acctggctga cgtgcgcctc cccggcatac ctggcgcaga acgggactcc tcgctgcatc
     1921 tccgatctga gtgaacacaa ctgcatacgc ttcgtgtcgc cctccagctg ccgggtgcgc
     1981 gactggacct tcgacgtcga cggagggata acctgcgtta cgccggcggg taatctttcg
     2041 gtgacgtcac tggacggtgc agtcgtcgct gcgagaaccg gtgcaggcat tgcgcaagtt
     2101 cccgacgcac tggctttccg cgccataatg gccggggaac ttcggccgat acttacccgg
     2161 catatcgcgg aatccgtgcc gctcatgctc acgtatcccg gcaaccgcta ccttcctgct
     2221 aaggtgcggg ccttcggcaa atttttcgag aatgcctacc ccaaggcagg cttgtggccg
     2281 cagatcgccg cgaagctcga ggccgaaggg aggactgcgg cctagcgtgc ggtgcggccg
     2341 ccgccggagg gcgccggatc tatgccgaag tggcggtaga ggtcagcgag cagccaggcc
     2401 ggaccgatca ggaggaactg gacgtccttg aagaacgagg gcttcttacc ctcgacttcg
     2461 tgccctatga actggccga
//
LOCUS       DASUOM010000368         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_270616,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000368 DASUOM010000000
VERSION     DASUOM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270616"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VFY27_07715"
     CDS             complement(<1..1183)
                     /locus_tag="VFY27_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5765439.1"
                     /translation="MTFEIALVLAIMVAALILFVSALLRMDLVALLVLGALAVTGLVS
                     AEQAFDGFSNSAVITIWAMFILSEGLARTGIADIISRQVLRIAGRRPVPVVMVIMATA
                     ALLAAFMNNIGVAALMLPVVVDVARRTRIPQSLLLMPLAFATLLGGQTTLFTSHNLLV
                     SEALLRSGHQPFRVFDFTPVGGAIAIAGVLFIALAGRLLLPKKARTSRGRYRSQRSLR
                     ARYKLQEQTFMLRIPVGSVLVGKTLAQSRIGASTGLIILALFRFGRSETLPSRQTVLR
                     AGDNLLVQGRLDQFRELQRWSDLVIEREAPVLKSMVASKVAYAEVQIAEGSPLVGELI
                     RDAGFRSRYNVSVVGIHRNDRYRLTNLAYVPLRAGTRVLVQGEAEAIGQLEKFSDFSE
                     VE"
     gene            1418..>2310
                     /locus_tag="VFY27_07720"
     CDS             1418..>2310
                     /locus_tag="VFY27_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5765440.1"
                     /translation="MNVAAEPMADYLQTLNGAQRKAATYGHRSGDSFGSGPLLVIAGA
                     GTGKTMTLAHRVAHLIIQGVEPERILLLTFSRRAALAMTRRVEAIVRKALRESGRHRA
                     LRNNCTLPWSGTFHSVANRILRRYAHNLGLDPGFSILDRGDAADLLDVVRLELGHAAK
                     ARRFPKKDTCLAIYSRCVNAGTPLADALALTFPWCAEWGQELRTIFRRYTEVKQENQA
                     LDYDDLLLYWSHLVADAELAAEIGSWFDHLLVDEYQDTNLLQADILRALKPHGIGVTV
                     VGDDAQSIYSFRGADIENILG"
BASE COUNT          442 a          747 c          714 g          407 t
ORIGIN      
        1 gctcgacttc gctgaagtcc gaaaacttct ccagttgccc gatcgcttcc gcctcgccct
       61 gcacgagaac cctcgtgccg gcccgcagtg ggacgtacgc gaggttcgtc aaccggtacc
      121 tgtcgttgcg gtgtattccg acgacgctga cgttgtagcg gctgcggaaa ccggcgtcgc
      181 ggatcagctc gccgaccagc ggcgaccctt ccgcaatctg cacctccgcg tatgcgacct
      241 tgctcgcgac catcgacttc agcacgggtg cttcgcgttc gatcaccagg tcgctccagc
      301 gctgcaactc gcggaactgg tccagccttc cctgcaccag caggttgtcc cccgcgcgga
      361 gcacggtttg ccggctgggc aaggtttcgc tgcggccgaa gcggaacagc gccaggatga
      421 tcaggcctgt cgatgccccg atgcggcttt gggccagcgt cttcccgacg aggacagagc
      481 cgaccgggat ccgtaacatg aacgtctgct cctgcagctt gtagcgcgcc ctcaggctgc
      541 gctggctgcg atagcggccg cgcgacgtac gcgctttctt cggcagcagc agcctgccgg
      601 ccagcgcaat gaacagaacg ccggcgatcg cgatcgcgcc tccaaccggg gtgaaatcga
      661 atacacggaa gggctggtgc ccggaacgaa gcagggcttc gctgaccagc aggttgtgcg
      721 acgtaaagag ggtggtctgc ccgcccagca gggtcgcgaa cgcgaggggc atcaagagca
      781 gcgactgggg aattctcgtg cggcgcgcaa cgtccacaac caccggcagc atcagcgccg
      841 ccacgccgat gttgttcatg aaagcagcga gaagcgccgc cgtggccatg atgaccatca
      901 ccaccgggac cggccgccgc ccggcaatgc gcaggacctg gcggctgatg atgtccgcaa
      961 tgccagtgcg ggcgagcccc tcgctcagta tgaacatcgc ccagatcgtg atgaccgcgc
     1021 tgttactgaa accgtcgaaa gcctgttccg cagagacgag gccggtgacc gccagcgcgc
     1081 ccagtaccag caacgccacg aggtccatgc gcagcagcgc actgacgaac aggatcagcg
     1141 ccgcgaccat gatggcgaga acgagtgcga tttcgaaggt catgaacgcg tgcttggagc
     1201 ttattctctg cggtgcgtga cggggcaatc gtaaggacgc gatggtcgat cgatggcatc
     1261 aacggatgcg cagtgtacgc aaatcgaagc acggaatccg cgcaaagcca cggctgtcgc
     1321 cgttttgcgc cggcgcgcgg acttcccgct gcgaaagagc ggcgcttgta aattcatcca
     1381 gtactggata aaaagacaca tttgccggag tggctggatg aacgttgcgg cagagcccat
     1441 ggccgactac ctgcagacgc tgaacggcgc acagcggaag gctgctactt acggacatcg
     1501 atccggtgat tccttcggct ccggcccgct tctcgttatt gccggcgcgg gaacgggcaa
     1561 gaccatgaca ctcgcccaca gggtcgcgca cctcatcatc cagggcgtag agcccgagcg
     1621 catcctgctc ctgacgtttt cccgccgcgc cgcgctggca atgacgcgcc gggttgaagc
     1681 catcgtgcgc aaggcacttc gtgaatcggg tcggcaccgg gctcttcgca acaattgcac
     1741 cctgccatgg tccggcacct tccactcggt cgcgaaccgg atattgcgcc gttatgcaca
     1801 caatctgggg ctcgacccgg gcttttccat tctcgaccgc ggcgacgctg ccgacctgct
     1861 ggacgtggtg cggctcgaac tggggcatgc tgcaaaggcc aggaggtttc cgaagaagga
     1921 cacctgcctt gccatctatt cccgatgcgt gaacgccggc acgcctctcg ccgatgcgct
     1981 tgccctgact tttccatggt gcgccgagtg ggggcaggaa ctgcggacga tcttccgccg
     2041 ctatacagaa gtaaagcagg agaaccaggc gctggattac gacgacctgt tgctctactg
     2101 gagccatctc gtcgcagatg cggaactcgc cgccgagatc ggctcgtggt tcgatcacct
     2161 gctcgtcgac gagtaccagg atacaaacct gctgcaggcg gacatcctcc gcgcgctgaa
     2221 gccgcacggt atcggagtta ccgtggtcgg cgacgacgcc cagtctatct attcctttcg
     2281 cggcgccgac attgaaaaca tcctcggttt
//
LOCUS       DASUOM010000369         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_212262,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000369 DASUOM010000000
VERSION     DASUOM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212262"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFY27_07725"
     CDS             <1..999
                     /locus_tag="VFY27_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5765441.1"
                     /translation="LREEVNVAMNFGRIVGKSPALKHMLAQVEAVAQTPANVLILGES
                     GVGKELVARAIHARSPRAEGPLVKVNCASIPKDLFESEFFGHVKGAFTGAHRDRVGRF
                     QLADGGTIFLDEVGEIPQDLQGKLLRVLQESEFERVGDDVTRSVDVRVIAATNRDLEK
                     QVVEGKFREDLFYRLSVFPIEVPPLRQRREDVVQLAQHFLDVTCKDFGRKPMKLNRSQ
                     VHAIQEYDWPGNVRELKNVIERAVILSQGNVLRLDLSLPEAAISAEVPGEGSKQPGDA
                     VLTEKELKALQKKNLINALKQANWRVSGKTGAAELLGIRPTTLNDRIRAFGIKKPH"
     gene            1056..1682
                     /locus_tag="VFY27_07730"
     CDS             1056..1682
                     /locus_tag="VFY27_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5765442.1"
                     /translation="MANLITLARLLLLIVVVLIAYQPYSYWHFVNVVLLILVFVSDGI
                     DGYVARKRNETSVFGAMFDIASDRMTELTMWIVLVDLDIVAVWVPLIFIIRGTIVDTI
                     RASQVTTAKNAPFSMLETPMGKWLVAGKFMRAFYAALKATTFCWLLLIQPLPVLWPDI
                     FTGWMTLLRGIGAVLVYASVLTCLLRGFPVVWEFAYQEMDSFALKRVK"
     gene            1766..>2220
                     /locus_tag="VFY27_07735"
     CDS             1766..>2220
                     /locus_tag="VFY27_07735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5765443.1"
                     /translation="MPLAVFDVDGTLVSGRSTEKRFITWLLRRGHLGPFQMLSMVWFI
                     LRWFPRFGRHVFKKNKAYLNGLPVSLIANEAREFVASMPDHAWIAPALAELEMHKHRG
                     DVVVLLSGTLQPIVECLSARVGADNSVGTECLVERGRFTAAPPSRHPFY"
BASE COUNT          451 a          618 c          685 g          466 t
ORIGIN      
        1 ctccgcgaag aggtgaacgt cgccatgaac ttcgggcgca tcgtcggcaa aagccctgca
       61 ctgaagcata tgctggcgca ggtcgaagcg gttgcacaga ctcccgccaa tgtccttatt
      121 ctcggcgagt ccggcgtcgg caaggaactc gttgcccggg ccatccatgc aagaagtccc
      181 cgggcggagg gtccgctggt aaaggtgaat tgcgcctcca tcccgaaaga cctgttcgag
      241 agcgaatttt ttggtcatgt caaaggcgcg ttcactggcg cgcaccggga cagggtcggg
      301 cggttccagc tggctgacgg cgggactatc ttcctcgacg aggtgggaga gataccgcag
      361 gacctgcagg ggaagctgtt acgggttttg caggaaagcg aattcgaaag agtgggcgac
      421 gatgtcaccc ggtccgtcga cgtgcgcgtc attgcggcca cgaatcgcga cctcgagaag
      481 caggtcgtgg agggcaagtt ccgcgaggac ctgttctacc ggcttagcgt cttcccgatc
      541 gaagtgccgc cgttgcgaca gaggcgtgag gacgtagtcc agcttgccca gcacttcctc
      601 gacgtgacct gcaaggactt cggcaggaaa ccgatgaaac tgaatcgaag ccaggtccat
      661 gcgatccagg aatacgattg gccgggcaat gtcagggagc tgaaaaacgt aatagagagg
      721 gctgtgatac tttcgcaggg caatgtgctg cggctcgacc tgtcgcttcc ggaagcggcg
      781 atcagtgctg aagtcccggg cgaaggcagc aagcaaccgg gcgatgccgt gctgacggaa
      841 aaagaattga aggccctgca gaagaaaaac ctgatcaatg cgctcaagca ggcgaactgg
      901 cgcgtgtccg gcaaaacggg cgcggcggaa cttctcggca tacggcccac gacgcttaac
      961 gacaggatcc gcgctttcgg catcaagaag cctcattaaa cctcgactgg cgggtttagc
     1021 cgttccggcc gggccttcgc tatataatcg cagcgatggc gaacctgata acactggcgc
     1081 ggctcctttt actcatcgtg gtagtgttga tcgcctatca gccctactca tactggcact
     1141 tcgtcaacgt cgtcctgttg atcctggtct tcgttagcga cggaatcgac ggctatgtcg
     1201 cccggaagcg caacgaaacg agcgtctttg gcgccatgtt cgatatcgca agcgaccgta
     1261 tgacggaact gacgatgtgg atcgtactgg tggacctgga tatcgtggcc gtctgggtac
     1321 cgctgatttt catcattcgc ggcacaattg ttgatacgat tcgcgcgagc caggtaacga
     1381 ccgcaaagaa tgcgccgttc tcgatgctgg agacgccgat gggcaagtgg cttgtcgccg
     1441 gcaagttcat gagagcgttc tacgctgcgc tcaaggcaac gactttctgc tggctgctgt
     1501 tgattcagcc gctgccggtc ctgtggccgg atattttcac gggctggatg acgctcctcc
     1561 ggggcatcgg cgctgttctt gtttatgcat cggtgcttac gtgcttgctt cgcggttttc
     1621 cggtcgtgtg ggagtttgcg taccaggaaa tggatagctt tgctttgaaa agagtgaaat
     1681 gaaccgggct gtccatgaat cgccgtaata tccgcgggca gagaccaggg aagcgcacga
     1741 gcgaactcat gaactgtccg cattaatgcc gctagccgtt ttcgatgtag acggaaccct
     1801 ggtctccgga cgcagtacgg aaaagaggtt catcacgtgg ttgttgcgcc ggggtcacct
     1861 gggcccgttc cagatgctgt cgatggtctg gttcatcctg cgctggtttc cccgcttcgg
     1921 ccgtcacgtg ttcaaaaaga ataaagccta cctgaacggc ctccccgtat ccctgatcgc
     1981 gaacgaagcg agagagttcg ttgcgtccat gcctgatcac gcctggatcg ccccggccct
     2041 tgcggaactc gagatgcaca agcacagggg ggatgtcgtc gtgctgctgt cggggacgct
     2101 gcaacccatt gtggaatgcc tttctgccag ggtcggagcc gacaactccg tcggcaccga
     2161 gtgcctcgtt gagcggggcc gcttcactgc cgcccctccg tccaggcacc ctttctacga
//
LOCUS       DASUOM010000370         5671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_772834,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000370 DASUOM010000000
VERSION     DASUOM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772834"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..3540)
                     /locus_tag="VFY27_07740"
     CDS             complement(64..3540)
                     /locus_tag="VFY27_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765444.1"
                     /translation="MPNKPNLRTVPAEEQDLRARLEKAEDSLREILSGESDAVFIEGA
                     GGAQLFAVKGADQSYRTLIENMSEGALILTPEGLILYANRRFAEMLGTPLEKVIGSEI
                     HRWFAPEGRGVLQTLLRKDASDRRREELALTAADGTQVPVNLSVSHLKLEGAPASFCM
                     VATDLTQQKHNEAILAAESLARAILEQAADAIVVCDETGRVIRASRQAQAFCGNNPVG
                     QHFEHAFPLRQPGGTTFSTIGDFGTNPRQSVEARLERDGQGFDLLVSVGHLKGAGTGF
                     VGSVVTLTDVTERVRAERALLESGQRYRMLFESMDEGFCIAEMLNDPDGKAVDYRFVE
                     INPAFEKHTGLHQAVGKTARQLVPGNDAHWFDIYGKVARTGEAIRFESPADAMHRFFD
                     VFAFRIGGDGSRNVGILFRDITARKHSDTALRESELRYHSLFENMLEGYAYCRTIFEQ
                     DRLSDFIYLEANGAFGRLTGLQDVVGKKVSDVVPGVQKDNPELFEIYEQVALTGQPKK
                     FETYMPALSRWLAITVYSSDREHFVAVFENITDRKNSEARILYLNRVYAMLGGINTLI
                     RRVHDRDELFREACRIAVEAGGFRMVMIAILDRSTMNIVPVASAGMDAELKTAIKGLL
                     SSSEGASKTMIARAIRGKKPVVSNDSLNDPQNLLGKQHGEGGVRSVAVLPLVVSDEAV
                     GVLALYAGEVEFFHEEEMKVLTELASDIAFAIDHIEKGDKLSYLAYYDPLTGLANRSL
                     FLERMAQHLRSAAGGRHKLAVFLIDLERFKNINDSLGRPAGDALLLQVAQWLTRNSGD
                     AKLLARVGPDHFAAVLPEVRQDGDIAQLLEQWMKAFLDHPFRLKDAVFRIAIKVGVAL
                     YPDDGADAETLFRNAEAALKKAKAGGDRYLFHMQNMTEAVAVKLTLENQLREAFDREE
                     FVLHYQPKVNLVSGKLTGAEALIRWNDPRTGLVPPGRFIPILEETGLIFEVGRWALRK
                     ALEDYLRWRNAGLAAVRVAVNVSPLQLRNRGFIAEIQQAIGTDAQAAAGLELEITESL
                     IMENVRHSTASLQAIRALGVTIAIDDFGTGFSSLAYLAKLPVDTLKIDRSFVTDMTVG
                     PEGLALVSTMINLAHSLKLKVVAEGVETEEQSRLLRLLDCDEMQGFLFSKPVPREIFE
                     TRFLKLLPHPSG"
     gene            complement(3540..3845)
                     /locus_tag="VFY27_07745"
     CDS             complement(3540..3845)
                     /locus_tag="VFY27_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5765445.1"
                     /translation="MKTALRAAASSRYTLILYVAGQSPKSLNAIGNIKRICEEHLQGR
                     YQLEVIDLYQQPQLAQGEQIIAVPTLLRKLPSPLRRIVGDLSNTERVLVGLDLRSAP"
     gene            complement(3878..5635)
                     /gene="kaiC"
                     /locus_tag="VFY27_07750"
     CDS             complement(3878..5635)
                     /gene="kaiC"
                     /locus_tag="VFY27_07750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX5765446.1"
                     /translation="MAVPNLKRNSPTRPARPRRQLPKSPTCIQGLDEITGGGLPKGRP
                     TLVCGGAGCGKTLLAMEFLVRGATQFDEPGVFMAFEETAKDLTQNVVSLGFDLKDLVA
                     RKQIVLDFVHVERSEIEVSGEYDLEGLFVRLGLAIDSVGAKRVVLDAVESLFAGLPNQ
                     AILRAELRRLFRWLKDKGVTAIVTGERGDGMLTRQGLEEYVSDCVIVLDHRVSEQTSS
                     RRLRVVKYRGSTHGTNEYPFLIDEDGISVLPVTSLSLEHRVSTERVSTGIARLDAMLG
                     GKGYYRGSSVLVSGTAGTGKSSIAAHFADAACRRGERVIYSSFEESSSQVIRNMRSIG
                     IDLEPWVKKGLLLIRAKRPSSAGLETHLALKRKAINDFKPQVMILDPINSFIVGGNEI
                     GVQSMLMRLLDFLKTNLITGLFTNLTPGGSVIEHTEVSISSLIDTWLLLRAIDSAGER
                     NRGLSILKSRGMAHSNQIREFLLTDHGVELRDVYVGASGVLTGSARLTQEAQDRAQQM
                     LHKQEIERRQLDLKSKHQALEAQIAALRAEFEPQEAELRKIIGQEQAQGAQLAQGRVD
                     MGLSREEDAAVKKPERGSQ"
BASE COUNT         1035 a         1794 c         1654 g         1188 t
ORIGIN      
        1 gttgacgcga aggcgctgcc ctcgatttcc aacggactct gcgttgcatc cttcatggct
       61 gcgttaaccg gaagggtgcg gaagcagttt caggaatctt gtttcgaaaa tctccctcgg
      121 caccggcttg ctgaacagga agccctgcat ttcgtcgcag tcaagcaagc gcagcaagcg
      181 cgactgctcc tcggtctcca cgccctccgc caccaccttg agtttgagcg agtgcgccag
      241 gttgatcatg gtggacacca gcgccagtcc ctccggcccg acggtcatgt cggtgacaaa
      301 cgagcggtcg atcttcagcg tgtccactgg cagcttggcc aggtaggcga gcgaggagaa
      361 gcccgtgccg aagtcgtcga tggcaatggt cacgcccagc gcgcggatcg cctgcaggct
      421 ggcggtgctg tgcctcacgt tctccataat caggctctcg gtgatctcca gttccagtcc
      481 cgccgccgcc tgcgcgtcag tgccgatggc ctgctggatc tcggcgataa agccgcggtt
      541 gcgcaactgc aggggtgaca cattcaccgc gacgcgcacg gccgccaggc ccgcgttgcg
      601 ccagcgcaga tagtcttcga gggctttgcg caacgcccag cgtccgactt cgaagatcaa
      661 tccggtttct tccagaatcg ggatgaaccg gcccgggggc accaggccgg tgcgcggatc
      721 gttccaccgg atcagcgcct cggcgccggt caacttgccg ctcacaaggt tcaccttggg
      781 ttggtaatgg agtacgaatt cctcccggtc gaacgcctcg cgcagctggt tctccagcgt
      841 gagctttacg gccaccgctt cggtcatgtt ctgcatgtga aacaggtatc gatcgccgcc
      901 cgccttggcc ttcttgagcg ctgcctcggc gttccggaac agggtttcgg cgtcggcacc
      961 gtcgtccggg tacagcgcga cgccgacctt gatggcgatc cggaacacgg cgtccttcaa
     1021 gcgaaacggg tgatccagga acgctttcat ccattgctcg agcagctgcg caatgtcgcc
     1081 gtcctgcctg acttccggca ataccgcggc gaaatggtcc ggccccaccc gcgccagcag
     1141 cttggcgtct cccgagttgc gcgtcagcca ttgcgccacc tgcagcagga gcgcgtcgcc
     1201 ggcgggccgg ccgaggctgt cgttgatgtt cttgaaccgt tccaggtcga tcaggaacac
     1261 ggccaacttg tggcggccgc ccgctgcgct gcgcaggtgc tgcgccatcc gctcgagaaa
     1321 caggctgcgg ttggcgagcc cggtgagtgg atcgtagtag gcgaggtagc tgagtttgtc
     1381 ccctttttcg atgtgatcga tcgcgaacgc gatgtcgctg gccagctcgg tcagcacctt
     1441 catctcttct tcatggaaga actcgacctc gccggcatac agggccagca cgccgacggc
     1501 ttcatccgag acgaccagcg gcaacacagc caccgagcgg actccgcctt cgccgtgctg
     1561 cttgccgagc aagttctggg ggtcgtttag cgaatcgttg gacaccacgg gctttttccc
     1621 ccggattgcg cgcgcgatca tcgttttcga cgcaccctcg ctggaggaca aaagtccttt
     1681 gatggccgtc ttgagctccg catccatgcc agcggacgcg accggcacaa tattcatcgt
     1741 gctccggtcc aagatggcga tcatgaccat gcgaaagccg cctgcctcga ctgcgatgcg
     1801 gcacgcctct ctgaacaact cgtcgcgatc gtgcacgcgc ctgatcagcg tgttgatgcc
     1861 gccgagcatc gcatagacgc ggttgaggta gagaatcctg gcttctgaat tctttctgtc
     1921 cgtgatgttt tcgaacaccg ccacgaagtg ctctctgtcc gaactgtaga cggtaatggc
     1981 aagccatcta ctcaatgcgg gcatgtacgt ttcgaatttt ttcggctggc cagtcaaagc
     2041 cacctgttca tatatctcga agagttccgg attatccttc tgcacaccgg ggacgacatc
     2101 gctgaccttc ttgccaacca catcctgcaa accggtgagc ctgccaaacg cgccattggc
     2161 ttcgaggtag atgaaatcac tcagcctgtc ttgttcaaag atcgttctgc aatatgcata
     2221 accttccagc atattttcaa acagggagtg atagcgcaat tcgctttccc tgagcgcagt
     2281 atccgaatgt ttgcgggcgg tgatatccct gaagaggatt cccacattcc ggcttccatc
     2341 gccaccgatg cggaacgcaa atacatcgaa aaaccgatgc atcgcgtcag cgggactctc
     2401 aaaccgaatg gcttcacccg tccgggccac cttcccgtag atgtcaaacc agtgagcgtc
     2461 attgccggga accaattggc gcgccgtctt gcccacggct tgatgcagcc ccgtatgctt
     2521 ttcgaacgct ggattgattt ccacaaagcg gtagtcaacc gctttgccgt ccggatcatt
     2581 caacatctcg gctatgcaaa aaccctcatc catcgactcg aacagcatgc gataccgttg
     2641 cccgctctcc agcagcgctc tctccgcccg cacgcgctcg gtgacgtcgg tcagcgtaac
     2701 cacgctacca acgaacccgg ttcctgcccc cttcaggtgc ccgacactta ccaaaagatc
     2761 gaatccctgc ccgtcgcgct ccagcctcgc ctcgaccgac tggcgcgggt tcgtgccgaa
     2821 gtcgccgata gtggagaacg tcgtgccacc cggctggcgc aggggaaagg cgtgctcgaa
     2881 gtgttgtcca acggggttat tgccgcagaa tgcctgcgcc tgcctgctgg cgcgtataac
     2941 gcgcccggtc tcgtcgcaga ccacgatcgc gtcggcggcc tgttccagga tcgcgcgcgc
     3001 gaggctttcg gcagcaagaa tcgcctcatt gtgtttttgc tgtgtcaggt cggtcgcgac
     3061 catgcagaag gaagcgggcg ccccttccag cttcaagtgg cttaccgaaa gattgactgg
     3121 cacctgtgtt ccgtcggcgg cggtgagggc cagttcctca cggcggcggt cggaagcgtc
     3181 ctttcgcagt agcgtctgca ggactcccct gccttccggt gcaaaccagc ggtgaatctc
     3241 ggagccgatc accttttcga gcggcgttcc gagcatctcg gcaaagcgcc gattggcgta
     3301 cagaatcagg ccctccggcg taaggatgag cgcgccttcg ctcatgttct cgatcaatgt
     3361 ccggtaggat tgatccgctc ccttgaccgc aaatagctgc gcgccgcccg cgccttcgat
     3421 gaacactgca tccgactcac cgctgagaat ttcgcgcagg gaatcttcgg ctttttcaag
     3481 ccgggcgcgc aggtcttgct cttctgcggg aacggtccga aggttgggct tgttgggcac
     3541 tacggcgcac ttcgcagatc cagacccacc agcacccgct cggtgttcga cagatcgccg
     3601 acgatgcggc gcaaggggga cggaagtttt cttaacagcg ttggaacggc gatgatctgt
     3661 tcgccctgag cgagttgcgg ctgctggtac agatcgatca cctccagctg gtagcgtcct
     3721 tgcaggtgtt cttcgcaaat cctcttgata ttcccgattg cgttcagcga tttaggggac
     3781 tggccggcaa cgtacagtat caaggtgtag cgggatgatg ccgctgcgcg caacgctgtc
     3841 ttgatcttgg ggtgcccacg cctgcccggt ttcgccttca ctgcgatccc cgttctggct
     3901 tcttcactgc cgcgtcctct tcgcggctca agcccatgtc gacacgaccc tgcgccagtt
     3961 gagcgccctg cgcctgttcc tgcccgatga tcttccgcag ctcggcttcc tgcggttcga
     4021 actcggcgcg cagcgcggcg atctgggctt ccagcgcctg gtgcttgctc ttcagatcga
     4081 gttgtcggcg ctcgatttcc tgcttgtgca gcatttgctg ggcgcggtcc tgcgcttcct
     4141 gcgtcagccg cgccgagccg gtcagcacgc cgctcgcgcc gacgtacaca tcgcgcaatt
     4201 ccacgccgtg atcggtgagc aagaactcgc ggatctgatt cgagtgcgcc ataccgcgtg
     4261 atttcaggat ggacaagccg cgattgcgtt caccggcgct atcgatggca cgcagcagca
     4321 gccaggtgtc gatcaaggac gaaatggaaa cctcggtgtg ctcgatcacg ctgccgcccg
     4381 gcgttagatt ggtgaacaag ccggtgatca gattggtttt aagaaaatcc agcagccgca
     4441 tcagcatcga ttggaccccg atctcattgc ccccgacgat gaaactgttg atgggatcca
     4501 ggatcatcac ctgcggcttg aagtcattga tcgctttacg cttcagcgcc aggtgcgtct
     4561 ccaggcccgc ggacgagggg cgtttcgcgc gaatcagcag cagacccttc ttgacccacg
     4621 gctccaggtc gatcccgatg gaccgcatgt tgcggatgac ctgactggac gattcttcaa
     4681 aggacgagta gatgacgcgc tcgccgcgac ggcaggcagc atcggcaaag tgcgcggcga
     4741 tgctgctctt gccggtgccc gccgtgcccg agaccagcac gctgctgcct cggtaatagc
     4801 cctttccgcc cagcatggcg tcgagtcgcg caatgccggt ggaaacacgt tccgtggaga
     4861 cgcggtgctc cagggagagc gaagtgacag gcaggaccga aatgccgtct tcgtcgatca
     4921 gaaacggata ttcgttggtg ccgtgggtag agccgcgata cttgaccacg cgcaagcgcc
     4981 gcgaggacgt ctgctcgctc acgcgatggt cgagcacgat cacgcagtcg gagacgtatt
     5041 cttccaatcc ctggcgcgtc agcatcccgt cgccgcgttc tccggtgacg atggcggtaa
     5101 cacccttgtc tttcagccag cgaaacaggc ggcgcaattc ggctcgcaga atggcctggt
     5161 tgggcaagcc tgcaaacaac gattcgaccg catcgagcac gacacgcttg gcgccgacgg
     5221 aatcgatggc caggcccagg cgaacgaaca gtccttccag atcgtattcg ccgctcactt
     5281 cgatctcgct gcgctcgacg tgcacaaaat ccagcacgat ctgtttgcgc gcgaccaggt
     5341 ctttcaggtc gaaaccgagc gagacgacat tctgggtcag gtccttggca gtttcttcga
     5401 acgccatgaa cacgcccggc tcgtcgaact gcgtggcacc gcgcaccaga aattccatcg
     5461 cgagcagcgt tttgccgcag cccgcgcccc cgcacaccag ggtcggcctc cccttgggta
     5521 atccgccgcc ggtgatctcg tccaatccct ggatgcaggt gggagatttt ggaagttggc
     5581 gccggggtct ggctggcctt gtcggtgaat ttcgtttcag gttgggtacg gccatgatgg
     5641 atacctttat tggatcacga gcttcctttc a
//
LOCUS       DASUOM010000371         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1342223,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000371 DASUOM010000000
VERSION     DASUOM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342223"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFY27_07755"
     CDS             <1..844
                     /locus_tag="VFY27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5765447.1"
                     /translation="PIVPKPEDFDRLIPHVQRFYDSIARIRAPKVCAKTPIFRKAYLT
                     PARYRLLQIGIPVSRAAGKLGVDLPVAGAPGHLNDEMHLVWKSIESIGRLRLVIRAVP
                     AAKVIHLVRHPCGYIASVLRGESRRQFEDNRGASEDYGILEMLLDSTAARQRELSMAY
                     FRGLSPVERLAWRWVLFNDEAQEAAGESDHCRTVRYEDLCADPVRTGKELFLFTGLDW
                     QPQTEQFLRTSTTQEESSYYSVFKKPDIAAGKWREELDTEIVRRIKAVVQDTVSGRAY
                     ADDW"
     gene            834..2105
                     /locus_tag="VFY27_07760"
     CDS             834..2105
                     /locus_tag="VFY27_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5765448.1"
                     /translation="MTGKGRDTEVREELRILTFSTLYPNATMPQHGIFVEQRLRQLLS
                     RCRIRSRVVAPVPWFPLRSSFFGAYAAFAAVPKSECRHDVPILHPRYPLLPKFGMSSA
                     PLLMASAAYRTMKACLQDSIGFDLIDAHYFYPDGVAAALLGQWLGKPVTITARGTDVN
                     LIPEYRVPRKWMLWAAKRCSKIITVSNSLKEKLTEIGVAADMIETLRNGVDLEFFRPR
                     PDREKLRSELRMTCPTLLSVGNLIELKGHHVVIEALATLPGVNLVIVGGGPMKDDLAR
                     LAQRHDVQQRVTFAGVLTQQELVKYYCSADALVLASSREGMANVLLEALACGTPVAAT
                     PYGGNPEVVADRRAGILTASRTPDAVAEAVRSLLESPPDRAAVRGYAETFDWARTSDG
                     QFRIFRDAAAWAAESRDLGGRRHRASADEGD"
     gene            2110..>2500
                     /locus_tag="VFY27_07765"
     CDS             2110..>2500
                     /locus_tag="VFY27_07765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5765449.1"
                     /translation="MNKLAERAARGRFAWRALERLILGRSRTPRASVLIFHRVLRERD
                     SLRPNEPIIEEFETLIRFLADNFRVRLLREAVADVADGSDDGHSVAITFDDGYADNVD
                     NALPVLRKYGVPATFFITSDTLETGVMW"
BASE COUNT          468 a          728 c          836 g          468 t
ORIGIN      
        1 gccgatcgtg ccgaagccgg aggacttcga tcgtttgatt ccgcatgttc agcgcttcta
       61 cgattcgatc gcccggatac gggcgcccaa ggtctgcgcg aagacaccga ttttcagaaa
      121 agcgtacctg acgccggctc gctatcgcct gctgcagatc ggcattccag tgtcgcgcgc
      181 tgccgggaaa ctgggcgtgg acctgccggt ggcaggcgcg ccgggtcacc tgaacgacga
      241 gatgcacctg gtgtggaagt ccatcgaatc tatagggagg ctgcgactgg tcatacgcgc
      301 cgtgccagcg gcgaaagtga ttcatctcgt acggcacccc tgcggctaca tcgcatcggt
      361 gcttcgcggc gaatcgcgcc gacagttcga ggacaaccgc ggggccagcg aggactatgg
      421 catcctggag atgctgctcg attcgaccgc cgcgcggcaa cgcgagctgt cgatggcgta
      481 tttccgaggg ctgtcgcccg tcgagcggct cgcctggcgc tgggttctat tcaatgacga
      541 agcgcaagag gcggccgggg agagcgacca ctgcagaacc gtgcgctacg aagacctgtg
      601 tgccgacccc gttcgcaccg gtaaggagct gttcctgttt acgggactcg actggcagcc
      661 acaaacggag cagttcctgc gtaccagcac aacgcaggag gagagcagtt actacagcgt
      721 cttcaagaag ccggacatcg cagcgggcaa gtggcgcgag gagctggaca cggagattgt
      781 gcgccggata aaggctgtgg ttcaggatac cgtctccggc cgggcgtacg ccgatgactg
      841 gtaagggccg cgacaccgaa gtgcgtgagg agttacgcat actgacgttc agtacgctct
      901 acccgaatgc gacgatgccg cagcacggca tcttcgtcga gcagcgcttg cgtcagttgc
      961 tgtcgcggtg ccggatacgt tcgcgcgtgg ttgcgccggt gccgtggttc ccgctcaggt
     1021 cctcgttttt cggagcgtac gcggcgtttg ccgcggttcc gaagtccgaa tgtcggcatg
     1081 acgtcccgat tctgcatccg cgatatccgc tgctgccgaa gttcggcatg agttctgcac
     1141 cgttgctgat ggcatcagcg gcatatcgta cgatgaaagc ctgcctgcag gattcgatcg
     1201 gattcgacct gatcgatgcg cactacttct atcccgacgg cgtcgccgct gcgcttcttg
     1261 gtcagtggct cggtaagccc gtgaccatca cggccagggg cacggatgtc aatcttattc
     1321 ctgagtatcg cgtgccgcgc aaatggatgc tgtgggcagc gaaacggtgt tcgaagatca
     1381 ttacagtcag caattcgctc aaggagaagc tcaccgaaat tggcgtcgcg gccgatatga
     1441 tcgagacgct gcgaaacggc gtcgatctgg agtttttccg gccgcggccg gaccgggaga
     1501 agctcaggag cgagctgagg atgacctgcc cgacattgct ctcggtcggc aatctaatag
     1561 agctcaaggg gcatcacgta gtcatcgagg cgctcgccac gcttcctggc gtgaatctcg
     1621 tcatcgttgg cggcggaccg atgaaagatg atcttgcccg ccttgcgcaa cggcacgacg
     1681 tacagcagcg ggtcacgttc gccggcgtgc tgacgcaaca ggaactcgtg aagtactatt
     1741 gttcggccga tgctctcgtc ctcgcatcca gccgggaagg catggcgaac gtgctgctgg
     1801 aggcgctggc ctgcggaacg cctgtcgcag cgacgccata cggtggcaac ccggaggtgg
     1861 ttgcagatcg gcgcgcgggg attctgacgg cgagccgtac gccggacgcg gtcgcagagg
     1921 cggtgaggag cctgttagag agtccccctg atcgtgctgc cgtgcgtggc tacgccgaga
     1981 cgttcgactg ggctcggacc agcgacggcc agttcaggat atttcgcgac gctgccgcat
     2041 gggcggcaga aagccgcgac ctgggcgggc gccggcaccg ggcgtctgcc gacgagggtg
     2101 actgaacaca tgaacaagct ggcggaaaga gccgcgcgtg ggcgatttgc ctggcgggcg
     2161 ctggagcgcc tgatactggg acgttcccgg acgcccaggg catccgtcct gatcttccac
     2221 cgggtcctgc gcgagcggga ttcgctgcgg ccgaacgaac cgatcatcga ggagttcgaa
     2281 acgctcatca ggtttctcgc cgacaacttc cgggtgcgct tgctgcgcga agcggtcgcg
     2341 gacgtggccg atggcagcga tgacggccac agcgtggcga ttacattcga cgacggttat
     2401 gcggacaacg tggacaatgc gctgccggtg ctgaggaagt acggtgtccc agcaacgttt
     2461 ttcatcacca gcgacacgct ggaaactgga gtgatgtgga
//
LOCUS       DASUOM010000372         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1307182,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000372 DASUOM010000000
VERSION     DASUOM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307182"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFY27_07770"
     CDS             <1..741
                     /locus_tag="VFY27_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765450.1"
                     /translation="GRIDNARMQELNRAALDRDTSIVEVASMLLTDAGLVDREIANPS
                     FLPQLWRNTQRHLKLTFIALALAIFTGVGIAILVHRHKTATNAFLYFAGLMQTIPSIA
                     LLALLIPFVGVGQRPAIIALFLYSLLPIARNTVTAMQTIPAAYRQVAAAMGMTRRQEL
                     RHVLMPLAMPHVLAGIRTAAIISIGTATLAAFIGAGGLGDPIVTGLALNDSSLILRGA
                     IPAACLAILAELMFNALERWLVVPHMRL"
     gene            complement(831..2723)
                     /gene="htpG"
                     /locus_tag="VFY27_07775"
     CDS             complement(831..2723)
                     /gene="htpG"
                     /locus_tag="VFY27_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX5765451.1"
                     /translation="MTTVKGPETLGFQTEVRQLLHLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALAAPDLLGGEPDLGIIVEYDQDNGKISVTDNGIGMSRDEVIENLGTIAR
                     SGTAEFLKQMTGDSRKDASLIGQFGVGFYSAFIVADKVVVETRRAGLAASEAVRWESD
                     GQGEFTIEGIERSERGTRVTLHLKPEEKDFSNELRLTTLIRKYSDHIGFPVTLRKVGA
                     SSDDVKTVNSATALWTRSRSEISDEEYKEFYKHLSHDFADPLTWSHNHVEGKREYVSL
                     LYVPAKAPYDLWNREAPRGLKLYVQRVFIMDQAEQFLPLYLRFIKGVIDSSDLPLNVS
                     RETLQHSSQHEAIRGALTRRVLDMLTRMAADEPDKFATVWEEFGEVLKEGIVEDPQNA
                     DKLVKLLRFATTRSPSEKQDQSLADYVSRAASDQDKIYYLIAENYAAAKASPHLEQLR
                     EKDIEVLLLTDRIDPWIVDHLPQFEGKSLHDVGRGNLKLPDGEGDITQQAKNDEQKPL
                     LKKIKKTLKDRVEAVNVSMRLVESPACVVASEQDLTPQLRRMLESAGQKLPESKPVLE
                     INLEHPLVRKLSAERDEKRFAALCNIILDHALLAEGAQLENPAAYVQRMNQFLLDIGT
                     EAEATA"
     gene            complement(2900..3163)
                     /locus_tag="VFY27_07780"
     CDS             complement(2900..3163)
                     /locus_tag="VFY27_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765452.1"
                     /translation="MECLLQYLDDLDDFVGILALAAERMRQFVRTAILVSVTLCVQLF
                     GIFLALTQPPLALAAVSLLSAGLLYRAAVNNPVNRSLQTTDPV"
     gene            complement(3170..3400)
                     /locus_tag="VFY27_07785"
     CDS             complement(3170..3400)
                     /locus_tag="VFY27_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765453.1"
                     /translation="MKSPAMMDKNLNNDLRFATLKNEATRQRWELFHQAIAFAKQSNA
                     EAMEKEQFLKDSIAPVSDTARHRAEPLLSIAR"
     gene            complement(3581..3832)
                     /locus_tag="VFY27_07790"
     CDS             complement(3581..3832)
                     /locus_tag="VFY27_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765454.1"
                     /translation="MQDTREINPTSGSEAEPDTVSKERDAGLHSGDSTDADGWQQYRK
                     WISTAPAPRTRRGSVDPSLYTWKGYRSWTEQIKRNWSDS"
     gene            complement(4014..>4357)
                     /locus_tag="VFY27_07795"
     CDS             complement(4014..>4357)
                     /locus_tag="VFY27_07795"
                     /inference="COORDINATES: protein motif:HMM:NF023320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine/purine nucleotide monophosphate
                     nucleosidase domain-containing protein"
                     /protein_id="HEX5765455.1"
                     /translation="YFNWRLAIDHDFQKPFRATHETMASLDLNEDLPVHILAANLRRA
                     FSGIVSGNVREDTIAQIEAHGPFDISGSPRIMTLLDKLLAAFVAQGRMKLAAESYTPC
                     YRVRVAGVEHG"
BASE COUNT          806 a         1311 c         1282 g          958 t
ORIGIN      
        1 ggccgcattg ataacgcgcg aatgcaggaa ctgaatcgcg ccgctctcga cagggatacc
       61 agcatcgtcg aagttgcctc aatgctgcta acggacgctg gcctggtcga ccgggaaatc
      121 gccaaccctt cgttcctgcc ccaactctgg cgcaacacac aacgacacct gaagcttacg
      181 ttcatcgcac ttgcgctggc catcttcacg ggcgtgggaa tcgcaatact ggtacatcgt
      241 cacaagacgg cgacgaacgc cttcctgtat tttgctggat tgatgcagac gataccttca
      301 atcgcgctgc ttgccctgct catcccgttc gtcggcgtcg gccaaagacc ggcgatcatt
      361 gcgctgttcc tctactcgct gctgcctatc gcgcgcaata cggtgactgc gatgcagacg
      421 atcccggcgg cttaccggca ggtcgccgcc gccatgggca tgacgcgtcg gcaggagctt
      481 cgccacgtcc tcatgccgct cgccatgccc cacgtgctgg caggcatccg aacggcagcg
      541 attatcagca tcggcactgc cacgctggcc gcattcatcg gcgcgggcgg gctcggcgat
      601 cctatcgtca ccgggcttgc cctgaacgac agctcgctga tactgcgcgg agcgataccg
      661 gcggcttgcc tggcgatact cgcggaacta atgttcaacg cgctggagcg atggctcgtc
      721 gtgccgcaca tgcgtctgta ggagcgcgcg ccaggaacgc gcatgaagct cgcctggcgg
      781 tagttcacgg gcggagacga cgacattcgg cgtcgcccgc atagcggctc ttaagccgtg
      841 gcctctgcct ctgtgccgat gtccaggagg aattggttca tgcgctgcac gtaagcggcc
      901 ggattttcaa gctgagcgcc ctcggccagc agcgcgtggt cgaggatgat gttgcaaaga
      961 gcggcgaagc gtttctcgtc gcgctcggcg gacagcttcc tgaccagtgg atgttccagg
     1021 ttgatctcga gcacgggctt cgactcgggc agcttctgac ccgccgattc cagcatccgc
     1081 cgcagctggg gcgtcaggtc ctgctccgac gctacgacac aggccggcga ctccaccagc
     1141 ctcatgctga cgttcaccgc ctctaccctg tcctttaagg tcttcttgat cttcttgagt
     1201 agtggctttt gctcatcatt ctttgcctgc tgggtgatgt cgccttcgcc gtcgggcagc
     1261 ttcaggttgc cacggccgac gtcgtgcagc gacttgcctt cgaattgcgg caagtgatcc
     1321 acgatccacg gatcgatgcg gtcggtaagc aacagcacct ctatgtcctt ctcccgcagc
     1381 tgctcgagat gcgggctggc cttcgcagct gcataattct ccgcgataag gtaatagatc
     1441 ttgtcctggt cggatgctgc ccgggaaaca tagtcggcaa gcgactggtc ctgtttctcc
     1501 gaaggagaac gcgtcgtggc gaagcgcagc agcttgacga gtttgtccgc gttctgcggg
     1561 tcctctacga tcccttcttt cagcacttcc ccgaattctt cccacaccgt ggcaaacttg
     1621 tccggctcat ccgctgccat cctcgttagc atgtcgagaa cgcgccgcgt caaggcgccg
     1681 cgtatcgcct cgtgttggga actgtgctgc agcgtttccc gcgatacgtt caacggcagg
     1741 tccgaagagt cgatcacacc cttgatgaag cgcaggtaaa ggggcaggaa ctgctccgcc
     1801 tggtccatga tgaagacgcg ctgcacgtaa agcttgagtc ctcttggtgc ttcccggttc
     1861 cacaggtcgt aaggcgcctt cgcggggaca tagagaaggc tgacgtactc cctcttgccc
     1921 tcgacgtgat tgtggctcca ggtcagcgga tcggcaaagt cgtgggagag gtgcttgtag
     1981 aattccttgt attcctcgtc gctgatctcg cttcgcgaac gggtccatag tgcagttgcc
     2041 gagttgaccg ttttgacgtc gtcgctactt gcgccgacct tccgtagcgt caccggaaac
     2101 ccgatgtgat cggaatactt cctgatgagg gtcgtaaggc gcagttcgtt cgagaagtcc
     2161 ttttcttcag gcttcaggtg cagcgtcacg cgggtgcccc gctcgctgcg ctcaataccc
     2221 tcgatcgtga actctccctg tccgtccgac tcccagcgga cggcctcgct cgctgctagg
     2281 ccagcacgtc gcgtctcgac cacgaccttg tcggccacga tgaatgcgga gtaaaacccc
     2341 acaccgaatt gaccgataag gctggcatcc ttccggctgt ccccggtcat ctgcttcagg
     2401 aactccgcgg ttccggacct ggctatggtt cccaggttct cgattacctc gtcacgagac
     2461 atgccgatgc cgttatcggt cacggagatc ttgccgttgt cctggtcgta ttcgacgatg
     2521 atgccgaggt ccggctcgcc gcccagcaag tcgggcgcag ccagcgcctc gaagcgaagc
     2581 ttgtccgctg cgtcggaggc attggaaatc agttcccgga gaaaaatctc cttgttgctg
     2641 tagagggaat ggatcatcag gtgcagcaat tgccgcactt cggtctggaa ccccagggtt
     2701 tccgggcctt tcactgtagt catttgctat cactctcgac ggcaggcatg ccgcattgca
     2761 aatgtctgac accatggtgc cggatgggga attttcaaga gcgagccagc ggcaggagtg
     2821 accggaatcc gactgcggga actcgtcaac cgggtgagtt cgcccaggct gtttcgcact
     2881 cgcccgcgcg gtcgggctcc tacaccgggt cggtggtttg cagcgagcgg tttaccgggt
     2941 tattcacggc agcgcggtac aacaagccgg cggagagcag ggagaccgct gcgagcgcga
     3001 ggggaggttg cgtcaaagcc aggaatatgc cgaagagctg aacgcaaagc gtaacagaca
     3061 ccaggatcgc cgtcctgaca aactgccgca tccgttccgc cgcaagtgcg aggattccga
     3121 cgaaatcgtc gaggtcgtcg agatactgga gcaggcattc cattgccatc tacctggcga
     3181 tcgagaggag cggttccgcg cggtgccgag ccgtatccga caccggcgca atgctgtcct
     3241 tcaggaattg ttccttctcc atcgcctcgg cgttcgattg tttggcgaac gcgatggcct
     3301 ggtgaaacaa ctcccatcgc tgccgcgtgg cttcgttctt caaagtcgcg aatcgcagat
     3361 cgttgttgag gttcttgtcc atcattgcag gcgatttcaa agtgtgcaat tcagtttgag
     3421 cgactataga cccgtcgttc cacaactgcc gtgaggcact tcacagcctt aagtgtgtgc
     3481 gaaaaaacga caggattttt tgtggagttc gtcaaacaaa aaaggggaga aatttatttt
     3541 tttcctccca gggaaaaaaa tctgtcccct ttttccgtcc tcaggagtcg gaccagttgc
     3601 gcttgatttg ctcggtccag cttcgataac ccttccaggt atagagcgac gggtccacgc
     3661 tgccgcgacg ggttcgcgga gcgggcgcgg tagaaatcca cttccgatac tgctgccaac
     3721 cgtccgcatc agtggaatcg ccggagtgca gtcctgcgtc ccgttccttc gagactgtat
     3781 ccggctcggc ttcgctgcca ctggtcgggt tgatttccct ggtgtcctgc atacgagttg
     3841 ctcaagtatc cgacgccgca gatgctatgt cgcaggggtg cgcttgcaaa ggaaccgtgt
     3901 cacaggggcg agcggcactc cttccctgca gatcaggaat tcgagcgtgg ttttacttaa
     3961 cgggatgcgc cggccgcggg gcgcctggcg ttttgactaa acgtcgctcg ctgctatccg
     4021 tgctcgacgc cggcgacgcg gacacgatag cagggagtgt aactttccgc cgccagtttc
     4081 atgcgcccct gtgcaacgaa ggctgcaagc agcttgtcca ggagtgtcat gatccgcggc
     4141 gacccggaga tgtcgaacgg accgtgcgct tcgatctgcg cgatagtgtc ctccctgaca
     4201 tttccggaaa cgattccgga aaatgcccgg cgcaggttcg cggccaggat gtgtaccgga
     4261 aggtcttcgt tcagatcgag ggatgccatc gtctcgtgcg tcgcacggaa cggcttctgg
     4321 aagtcgtggt cgatcgcaag ccgccagttg aaataat
//
LOCUS       DASUOM010000373         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_375994,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000373 DASUOM010000000
VERSION     DASUOM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375994"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VFY27_07800"
     CDS             complement(<1..1634)
                     /locus_tag="VFY27_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5765456.1"
                     /translation="MPAPPSLHAIAAILFTLVAVFLFTREKLRLETTSLFVLITILVW
                     FEFFAIELNGVRVRAPDFLAGFGNEALITISVLMIVTKSLESTGALQPIGHVLAQLWS
                     VRPKLAFLATLLTAAGLSMFLNNTPVVAAVLPLLVAVSLQAGISPSGILMPVGFATII
                     GGMATTIGTSTNLLVVGIASDLGMRDLQMFDFAMPVFAVGGIAILFLWLIAPRMLPDR
                     RPPLTDIEPRVFESRLNIEPGSFADGKPLKDVLARCKGIKVTRIERGEITLSRMPLVV
                     LKEGDKLHVSDTPENLKEYERLLGATLLAADSKESVSSDHPLESDEHVAEVVVTSGSV
                     LERNTLDSTRLLSTYGLIPLALHRPGIPQRTEEALGGVTLEAGDVILVQGAPRALERL
                     HRSGSLLVLDGRIHLPRTTRASLSMIIMVGVVVVAATGLMRISVAASVGLALMILTNC
                     LTWREAVGAMDRRIVMVIVASLALGLALMATGATEYLASLYVAAAGGLSPALILAGFI
                     LLMALLTEIVTNNAIAVLGTPIAISVATQLGVSPEP"
     gene            complement(1792..3612)
                     /locus_tag="VFY27_07805"
     CDS             complement(1792..3612)
                     /locus_tag="VFY27_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5765457.1"
                     /translation="MPAPPSLHAIAAILLAFFAVFLFTRDKIRLESSSLLVLIVILVW
                     FEFFTIEYEGARVRAQNFLAGFGHEALITISVLMMLTKSLERTGALQPITHVLARLWT
                     VRPQLAFLATIVTAAILSMFLNNTPVVAAILPLLVAVSLKAGVSPTGILMPVGFATIV
                     GGMATTIGTSTNLLVVGIATDLGMGELQMFDFALPVFIVGGIAIVFLWLVAPRLLPDR
                     RPPLTDIEPRIFDSRLEIPKGSFADSKTLREILTKCKNMKVNKIERGDLALTKLPLVV
                     IRAGDSLHVSDTPENLKEYERMLGATLLAGDSQHTVSVDHPLESDEHVAEVVVTSGSV
                     LERNTLDGTRLLSTYGLTPLALHRPGVAQGKTTETLGSVTLEAGDVILVQGAPRAIER
                     LHRSGSLLVLDGRIHLPRTTKSTLSMIIMIGVVLVAATGLMRISVAASVGLTLMILTR
                     CLTWQDAVGAIDRRIVMVIVASLALGLALMATGSTEYIAALYIAATTGMSIIFILSGF
                     IFIMAFLTEIVSNNAIAVLGVPIAVSVAAQLSVPAEPFVIGLLYGANMSYIMPVGYQT
                     NLLVMSAGGYRFSDFARVGLPLKLIMWIGLSTGLVLAYGL"
     gene            complement(3758..4648)
                     /locus_tag="VFY27_07810"
     CDS             complement(3758..4648)
                     /locus_tag="VFY27_07810"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5765458.1"
                     /translation="MKTFRAAVRDSDFVITAQLPLDPGTTAGELRQILATLQPVVDAV
                     QIGDNPTTKPHMAPLAAAALSVQAGIDPIIQLTCRDRNRIALQSDVMGAAALGVTSLV
                     LSRGEKMPAMHKQRIKGVFDIGAKRLMATAQAVGANERLVSPPGFFLGSNVTIIDPPE
                     DWAAEGIGNKAGAGSKFVQTQPCLDLAALRKYMAALVARKALERVSVIVQVPLLESIE
                     ALHELQGARRPLLITKGLVERLQRAVDFFSEAEAVCSETLQALPTIPGVAGTNILHQG
                     GPAAVVRVLTAAFGDQGRHV"
     gene            complement(4648..>5757)
                     /locus_tag="VFY27_07815"
     CDS             complement(4648..>5757)
                     /locus_tag="VFY27_07815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5765459.1"
                     /translation="SQAPNLAVGQNGVVALSWLKADESGHRLQYSVLDGNAWSQPRDV
                     ARGENWFVNWADFPSVVPVSDEMWAAHWLVRQPAGGYAYDVHLSVSSDGGATWSESVM
                     PHDDNTATEHGFVTIFPEATGIGLIWLDGRNLAHGTSHHPSSGGMTLRAATYSGTLEA
                     ANEALVDELTCDCCQTDVATTNRGAIAVYRNRTTDEIRDIYAARYVDGEWLPGRAVAD
                     DGWTIGGCPVNGPTVAADGDHLAVAWFTAADDEAKVRLARSNDAGETWSAAVDVTEGE
                     LFGRAGVALLDRGEVAVSWLCGESGRHARLCLRRVAADNALGQIHVVSGEDEVPPLSV
                     PQLARSGDSIVAAWTVWDGDLTAVRSSRIAVASLR"
BASE COUNT         1156 a         1779 c         1769 g         1053 t
ORIGIN      
        1 aacggctcgg gagacacccc cagctgcgtc gccacgctga tagcgatcgg ggtgcccagt
       61 acggcgatgg cattgttcgt gactatctcg gtcaagaggg ccatcaacag gatgaagccg
      121 gccaggatga gggcggggga caggccgcct gccgcggcga catagagcga ggcaaggtat
      181 tccgtcgcgc cggttgccat cagcgcgagc cccagggcca ggctggcgac gatcaccatg
      241 acgatacgcc ggtccatcgc tcccacggct tcccgccagg ttaggcagtt ggtcagtatc
      301 atcagcgcca gccccacgga agccgcgacg gagattcgca tcaacccggt tgccgcgact
      361 acgacgacac ctaccatgat aatcatcgac aatgatgccc tcgtggtgcg cgggagatgg
      421 atgcgtccgt cgagcaccag caggctgccg ctgcgatgca ggcgttcgag agcccgggga
      481 gcgccttgca cgagtatgac gtcgccggcc tcgagcgtga cgccgcccaa cgcttcctca
      541 gtgcgctgtg ggataccagg ccgatgcagc gcaagtggaa tcagcccgta ggtcgacagc
      601 aagcgtgtgc tgtccagcgt attgcgttcc agcaccgatc ccgacgtgac cacgacttcg
      661 gctacgtgct cgtcgctctc gagcggatgg tccgaactca ccgattcctt cgagtccgcg
      721 gcgagaagag tcgcgcccag cagtcgctca tactccttca gattttcagg agtatcgctg
      781 acgtgcagtt tgtcgccttc cttgagaacg accaggggca ttcgcgagag cgtaatctct
      841 ccgcgctcaa tgcgcgtcac cttaatcccc ttgcagcgcg ccagtacgtc cttcagcggt
      901 ttcccgtcag cgaaagaacc cggttcgatg ttcagccgcg attcgaacac tcgcggctcg
      961 atatcggtca gcggcgggcg gcggtccggc agcatgcgtg gtgcgatgag ccacagaaac
     1021 agtatcgcga ttccgcccac agcgaatacc ggcatcgcga agtcgaacat ctgcaggtcc
     1081 cgcatcccga ggtcggaagc aattcccaca accaacaggt tcgtcgaggt cccgatcgtt
     1141 gttgccatac cgccgatgat cgtggcgaaa cctacgggca tcaggatccc cgagggagat
     1201 atacccgcct ggagcgacac tgcgacgagt agcggcagca cggcggcaac gacgggcgta
     1261 ttgttcagga acatgctgag tcccgctgcc gtcagcaggg ttgcaaggaa cgcaagcttc
     1321 ggccggacgg accacagctg cgccagtacg tgtccgatcg gctgcagcgc acccgtgctt
     1381 tccaggctct tggtcacgat catcaacacg gatatggtga tcagggcttc gttgccgaag
     1441 cccgccagga agtcgggcgc ccgcacgcgc accccgttca gttcaatcgc aaaaaattcg
     1501 aaccagacca gaattgtgat cagtacaaag aggctggttg tttccagtcg cagcttctcg
     1561 cgagtgaaca ggaacaccgc gactaaagtg aacaggatgg ctgcgatggc atgcaggctt
     1621 ggcggtgcgg gcaaagagtc gtgtcctcgg tcgggcgcac gccggctggc gctgcgtctg
     1681 gcacgaatgc tatccgactg gatcgctact tacgacgctt tttccgtggc ggttcgggct
     1741 tccccccggt cattcccggc cggacggcga ccagcgacgc gctaacgacg cctacaaacc
     1801 gtacgccagc accagcccgg tggagaggcc gatccacatg atcagtttca gcggcagtcc
     1861 tacccttgca aagtcggaga agcggtagcc gccggcactc atgacgagaa ggttggtctg
     1921 gtagccgacc ggcatgatgt agctcatgtt cgcgccgtaa agaagtccga ttacgaatgg
     1981 ctcggccggc acactgagct gcgcagcaac actaacggca atcggtacgc ccagcacggc
     2041 gatcgcgttg ttcgagacga tttccgtcag gaaggccatg atgaaaatga atccgctgag
     2101 gatgaaaatg atcgacattc ccgtcgtggc ggcaatgtaa agagcagcaa tgtattcagt
     2161 ggagccggtc gccatgagcg caaggcccaa cgcgagactg gcgacgatca ccatgacaat
     2221 tcgccggtca atcgcgccaa cggcgtcctg ccacgtgagg cagcgcgtca gtatcatcaa
     2281 cgtcagtccc acagacgccg ccaccgaaat acgcatgagt cccgtggccg cgacaagaac
     2341 cacgccgatc atgatgatca tcgagagcgt cgatttcgtc gtgcggggca gatggatgcg
     2401 tccatccagc acgagtagac tgccgctccg gtgcagccgc tcgattgcgc gcggcgcgcc
     2461 ctggaccagg atgacatcgc ccgcctcgag ggtcacgctg ccgagggttt ccgtcgtctt
     2521 cccctgcgca actcccggcc gatgcagcgc cagtggcgtg agcccatatg tcgaaagcag
     2581 gcgcgtgccg tcgagcgtat tgcgctccag tacggagccc gaggtaacga cgacttctgc
     2641 aacgtgctcg tcgctctcca gcgggtgatc gacgctgacc gtgtgctgtg agtcaccggc
     2701 cagcagcgtg gcgcccagca tgcgttcgta ttctttgaga ttctccggcg tatcactgac
     2761 gtggaggctg tcgccggccc tgataacgac gagcggcagt ttcgtcaacg caaggtcgcc
     2821 gcgctcgatc ttgttgacct tcatgttctt gcatttcgtc aggatctccc gcagcgtctt
     2881 gctatcagcg aacgaacctt tggggatctc gagccgtgaa tcgaaaatac gcggttcaat
     2941 gtcggtcaac ggcggccgtc ggtcggggag gagcctaggg gccaccagcc acaggaatac
     3001 tattgcaatt cctccaacga tgaagacggg gagggcgaag tcgaacatct gcagttcgcc
     3061 catgcccaaa tcggtcgcta tgccgaccac aaggagatta gtggatgtac ctatcgttgt
     3121 cgccatgccg ccgacgatgg tggcgaagcc tacaggcatc aggataccgg taggggaaac
     3181 tcctgccttc agcgacacag cgaccagtaa cggaagaatt gcggcaacca ccggagtgtt
     3241 gttgagaaac atgctcagga tcgcagcagt gacaatcgtc gccaggaagg cgagttgtgg
     3301 ccgcacagtc catagccgcg ccagcacatg ggtaatcggc tgcagcgcac cagtcctttc
     3361 gagactcttc gtcagcatca tcaggactga aatcgttatc agggcttcat gaccgaaccc
     3421 ggcgagaaag ttctgcgcgc gcacgcgcgc gccctcgtat tcgatggtga agaactcgaa
     3481 ccagacgagg attacgatca ggacgagcag actggacgac tccagccgga tcttgtcccg
     3541 cgtgaacagg aatactgcga agaacgcgag cagaattgcc gcgatggcat gcaggcttgg
     3601 tggcgcgggc aatgtatcgg tcctccgtgc gtcttcgcgc cgcgaggcgc tgcatcaggg
     3661 acggatgcta tcgaaccggg aagtgagttg cgatgccctc aatcgcaata tggtgagtaa
     3721 tatcgtccct gaggcgattc gacgtgatcc cggtagttca cacgtgacgc ccctgatccc
     3781 cgaacgcggc agtgagcacg cgcaccacgg cggctgggcc accctgatgg agtatgttgg
     3841 ttccagcaac gcccgggatg gtcggcagcg cctgcagtgt ttcggaacac actgcttcgg
     3901 cctcggaaaa aaagtcgaca gcgcgttgga gtcgctcgac caggccttta gtaatcagca
     3961 gcggccgccg cgctccctgc agctcatgaa gcgcttctat cgactccagt agcgggactt
     4021 gcactatgac ggagacgcgt tcgagtgctt tgcgtgcgac aagggcagcc atgtatttac
     4081 ggagtgcagc aaggtccagg cagggctgag tctgcacgaa cttgcttccg gcgccggcct
     4141 tgttgccgat gccctctgca gcccagtctt ccggcggatc gataatggtt acgttggagc
     4201 ccaggaaaaa gccggggggc gaaaccaggc gttcgttggc accgacggcc tgcgccgttg
     4261 ccatcagccg cttcgcgccg atgtcgaata ctccttttat tcgctgcttg tgcatggcag
     4321 gcattttctc gccgcggcta agtacaaggc tggtgacgcc gagcgctgcg gcgcccatca
     4381 cgtcgctttg caacgcgatg cgattgcgat cccggcaagt cagctggatg atcgggtcaa
     4441 tgcccgcctg taccgagaga gctgcggctg caaggggggc catgtgcggc ttcgtcgtcg
     4501 gattgtcgcc gatctggacg gcatcaacga ccggctgaag agtcgccagg atttgcctca
     4561 gctccccggc cgtcgtgccg gggtcgagcg gaagttgcgc ggtgatgacg aagtcgctat
     4621 cgcggacagc cgcgcgaaac gtcttcacta gcgcaacgag gcgacggcaa tgcggctact
     4681 gcggacggct gtgaggtcgc catcccacac cgtccacgcg gcgacgatgg agtcgccgct
     4741 tcgtgcaagc tgaggaacgc tcagcggcgg tacctcatcc tcgccgctga cgacatggat
     4801 ctgcccgaga gcgttgtctg cagcgactcg ccgcaggcac aagcgtgcat gcctcccgga
     4861 ttcaccgcac aaccagctga cagcaacctc accccggtcc agcaacgcga cgcccgcgcg
     4921 gccgaacagt tcgccttcag tgacatctac agccgcggac caggtttcac cggcatcgtt
     4981 cgagcgcgcc aggcgcacct tcgcctcgtc atcagccgcc gtgaaccacg cgacagcgag
     5041 atgatcgcca tcggccgcga cagtcggtcc gttcacgggg cagcctccga tggtccagcc
     5101 atcgtcagcg acggcgcggc cgggcagcca ttcaccgtca acatatcgtg cggcgtatat
     5161 gtctcttatt tcatctgtcg tacgattgcg gtagacggca atcgccccgc gattggtcgt
     5221 ggccacgtcg gtctggcagc agtcgcaggt caactcgtcc accagtgcct cgttcgcagc
     5281 ctcgagcgtg ccggagtagg tggcagcgcg cagggtcatt cccccgcttg atggatgatg
     5341 cgatgttcca tgggccaggt ttcgtccgtc gagccagatc agccctatac cggtggcttc
     5401 gggaaatatc gtgacgaagc cgtgttccgt cgccgtgttg tcatcgtggg gcatcaccga
     5461 ttcggaccat gtagcgccac catcggaaga gactgaaaga tgcacgtcgt acgcataacc
     5521 gccggccggc tgccggacca gccagtgcgc cgcccacatc tcgtcggaga cgggcactac
     5581 ggaggggaaa tccgcccagt tgacgaacca gttctcgcct cgtgcaacat cccggggttg
     5641 gctccacgcg ttaccatcaa ggacggagta ctgcagccgg tgcccgctct catcagcttt
     5701 caaccagctg agcgcgacga cgccgttctg gccgactgca agattggggg cctggct
//
LOCUS       DASUOM010000374         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1319741,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000374 DASUOM010000000
VERSION     DASUOM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319741"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /gene="ggt"
                     /locus_tag="VFY27_07820"
     CDS             complement(<1..1542)
                     /gene="ggt"
                     /locus_tag="VFY27_07820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5765460.1"
                     /translation="MSRIRYLLVTTLSAALLLTSMGAAAAGRNAVRGKHGVVASSSAP
                     ASEVGVETLRNGGNAVDAAIATAFALAVTWPTAGNIGGGGFLVYHGADGEATTYDFRE
                     KAPLAATEDMYLGEDGRVRNNSNHEGILAVGVPGTVAGLALAHREYGELEWSDLVAPA
                     VRLARDGIPITWKLHDDFKGREERWERYPSSAKVFLKEDGGFFEPGETWRQTDLANTL
                     ERIQREGRSGFYRGVTAALIADFMSKNGGLITLQDLEQYEAVEREPIRGSYRGYEIVS
                     MPPPSSGGVVLVEMLNILEGFDLTAAGHNSARYLHLLTESMRRAYADRARYLGDPDFN
                     EDMPIERLISKEHAESLRGSISLTGASVSNPALFADKYESDQTTHFSIVDKDGNMVSL
                     TYTLEWGYGSHIVVEGAGFLLNNEMGDFNAVPGITDESGLIGTEANRIRPEKRMLSSM
                     TPTIVARDGVPVFATGTPGGKTIINTTLQTILNIIDHGMTIAESIEAGRIHHQWLPDR
                     TLMEEH"
     gene            1730..3046
                     /locus_tag="VFY27_07825"
     CDS             1730..3046
                     /locus_tag="VFY27_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5765461.1"
                     /translation="MAETGRKSLTLDLYDLLTGDEDARVCREIPEVACREQPRNFFIH
                     IGSLVASKTGDRLASPKLVLSWLFTTLGAPTFMLGLLVPIRESLSLIPQLFVARAIRS
                     VAVRKWFWVAGSIAQGLAVVAMAIVAVMLRGAAAGWSILALLVVFSLARGVCSVAAKD
                     VLGKTISKSRRGTVTGYASSVAGAITILVGAFALAVQPGEHGLVLFVSLLLIAGGLWI
                     FGALVFSRLQEYPGATEGGGNAITEAVRQMRLIRDDRDLRQFLIVRTLLLSTALVAPF
                     YVSIASEHSGAALTQFGAMLIATGAASFLSAPAWGRMSDRSSRRVMAAAALLAAVAGF
                     LTAGLAQTPALPYPAIAFASTYFLLNIAHAGVRVGRKTHLIDIATPENRASYVAVSNT
                     FIGIMLIAGGVFGGIASAAGTTVVVLLLSAISLAAAAAAWRLEEAQ"
BASE COUNT          521 a          945 c          954 g          637 t
ORIGIN      
        1 gtgctcttcc atcaaagtcc gatcgggtag ccactgatgg tgaatgcggc cggcttcgat
       61 cgactcggcg atggtcatgc cgtggtcgat gatgttcagg atcgtctgca gcgtggtatt
      121 gatgatggtt ttcccccccg gcgttcccgt cgcgaacacc ggtacaccat cgcgcgcaac
      181 gatcgtcggg gtcatgcttg acagcatgcg cttctcagga cgaatccggt tcgcttcggt
      241 gccgatgagg ccactctcat cggtgatacc gggcacggcg ttgaagtcac ccatctcgtt
      301 gttcaggaga aaaccggcac cctcgaccac gatgtgcgat ccatagcccc actcgagcgt
      361 atacgtcagc gacaccatat tcccatcctt gtcgacgatg gaaaaatggg tcgtctggtc
      421 gctctcgtac ttgtcggcga agagggcggg gttgctcacg gatgccccag tcagtgaaat
      481 gctcccgcgc aggctttccg cgtgttcctt cgagatcagt cgctcgatcg gcatgtcttc
      541 gttgaagtcg ggatcgccga gatagcgcgc acggtccgca taggcccggc gcatggattc
      601 cgtgagcagg tgcaggtagc gcgccgagtt atgacccgcc gcggtcaggt caaagccttc
      661 aagaatgttg agcatttcca ccagtaccac cccgcccgaa ctcggtggcg gcatgctgac
      721 gatctcgtaa ccccggtagc tgccccgaat cggctcacgc tccaccgctt cgtactgttc
      781 gagatcctga agggtgatga ggccgccgtt ctttgacata aaatccgcga tcagcgccgc
      841 cgttaccccc cgatagaagc cggatcgccc ctcacgctgg atgcgctcca gcgtgttggc
      901 gaggtcggtt tgcctccagg tctcgccagg ttcgaaaaaa ccaccgtcct ctttcaggaa
      961 tacctttgcg gagcttggat acctctccca ccgctcctcg cggcccttga agtcgtcatg
     1021 caacttccag gtaatcggga tgccgtcgcg ggcgagacga accgccggcg ctaccaggtc
     1081 cgaccactcg agctcgccgt actcccggtg cgcaagcgcc agcccggcga cggtaccggg
     1141 aacgccgacc gcaaggatgc cttcgtgatt cgagttattc ctgacccggc cgtcttcgcc
     1201 gaggtacata tcctccgttg ctgcaagggg cgccttctcc cggaagtcgt aggtggtcgc
     1261 ctcgccatcg gccccgtgat agacgaggaa tcctccgcca ccgatatttc cggcagtcgg
     1321 ccaggtgacg gcgagggcaa acgccgtcgc tatcgccgcg tctacggcgt tgccgccatt
     1381 cctcagcgtc tcgacgccga cttcggacgc cggcgcggag gacgatgcaa ccaccccatg
     1441 cttgcctcgg accgcgttgc gtccggctgc ggcagccccc atggaggtga gcagcaaggc
     1501 ggcagacagc gttgttacca gcaaatagcg tattctggac ataagcatcg ttactgatcc
     1561 ggctccgtcg actggcagaa gaattgggac tagctgaacc tgaagtgccg cggattcgtg
     1621 ttgttgagct caaccgctcc tgcaggaaac ggcgcattct acaggatgcg accggctgag
     1681 gactgcagcg aacttcaata tgaaaccgca ccacgagagg cgctagccaa tggcggaaac
     1741 gggccggaaa tctctcacgc tggatctgta cgacctgctg acaggcgatg aggatgcgcg
     1801 cgtctgtcgt gaaataccgg aggttgcctg tcgggaacag ccgcgtaact tttttatcca
     1861 tataggctcg cttgttgcat ccaaaaccgg cgatcgcctt gccagtccga agctggtgct
     1921 gtcctggcta ttcaccactc ttggcgcccc gacgttcatg ctgggactgc tggtgccgat
     1981 acgcgaatcg ctatcgctga ttccccaact gtttgtcgcc cgcgcaatac gaagcgtggc
     2041 ggtgcgcaag tggttctggg tcgctggcag cattgcccag ggacttgccg tcgtggcgat
     2101 ggcgatcgtc gccgtgatgc ttcgcggcgc agcggcgggt tggagcatcc tggcgttgct
     2161 ggtcgtcttc agcctggcac gcggggtgtg ctctgtcgcg gcaaaggatg ttttggggaa
     2221 gaccatcagc aagagtcggc gcggcaccgt gaccggctat gcatcgtcgg ttgcaggcgc
     2281 cattaccatt ctggtcggcg cttttgcgct tgccgtgcag ccgggcgagc acgggctggt
     2341 tctattcgtg tcgctgctgc tgatcgccgg aggactgtgg attttcggcg cgctcgtttt
     2401 cagtcgactg caggaatacc cgggcgccac cgaaggtggc ggcaacgcca ttacggaggc
     2461 ggtgcgacag atgcgcctca ttcgcgacga tcgcgacctg agacagttcc tgatcgtccg
     2521 cacgttgctg ttgtcgacgg cgctggttgc gccgttctat gtgagcatcg cgagcgaaca
     2581 ttcgggcgct gccctgaccc agtttggtgc gatgctgatc gccaccggag cggccagttt
     2641 tctaagcgca ccggcctggg gccgcatgtc ggatcggtcg agtcgccggg tcatggcggc
     2701 ggctgcgctg cttgccgcgg tcgccggctt cctgacggcc ggacttgcgc agacgccggc
     2761 tcttccttat ccggcgatcg cgttcgcatc gacctacttc ctgctgaaca tcgcgcacgc
     2821 gggggtgagg gtgggacgga agacgcacct gatcgacatt gcgacgccgg agaaccgcgc
     2881 gagctacgtc gccgtgagca acacgttcat cggcataatg ctgatcgcag gcggtgtgtt
     2941 tggcggcatt gcctccgctg ccggcacgac ggtggtcgta ttgttgctct ctgcgatctc
     3001 tctcgccgcc gcggcggcgg cctggcgcct ggaggaggcg cagtaatctc cgctgta
//
LOCUS       DASUOM010000375         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1732870,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000375 DASUOM010000000
VERSION     DASUOM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732870"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFY27_07830"
     CDS             complement(<1..742)
                     /locus_tag="VFY27_07830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5765462.1"
                     /translation="MTRPVVKTEWHPASWQRLDAAQQPVYPDPAELERTVAELSRLPP
                     LVTSWEVDALKDLFAKAQRGDAFVLQGGDCAERFDDCTSDSIVAKLKILLQMSLVMLY
                     GMKKPVVRVGRMAGQYAKPRSADTETRDGTSLPSFRGDLVNRNPFSAADRVPNPDLIL
                     RGYERAALTLNFVRSLIDGGFADLHHPEYWDLDWVGHSKTADEYHEIVRSISNSLDFI
                     ESISGRAFYQTQRADIYSSHEGLHLYYEQ"
     gene            934..2103
                     /locus_tag="VFY27_07835"
     CDS             934..2103
                     /locus_tag="VFY27_07835"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765463.1"
                     /translation="MQDILVLGAGKIGALITGLLAECGDYRVQLADAVEGAAAGVAEA
                     HGLDPSCAFTFDAGNEQQLTEHVRKHKPVAVISGLPFYCNVAVAKVARAENLHYFDLT
                     EDVAVTNSVRALAKGATTAFVPQCGLAPGFISIAANELIKHFDKLRSVKLRVGALPQH
                     PNNVLKYSLTWSTDGLINEYGNPCQSIVEGQEINVLPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLGETYGARVNTMNYKTIRYPGHCEQMRLLMNGLKLNRDRATLKRILENSVPQTL
                     QDVVIIYAAVTGMQDGEFREENYVNKVYPEVVSGRLWSAIQITTAAGVCSVMDIVLSS
                     RPPRRGFIAQEEFGLQEILGNRFGEYYEHGGSNQLSADLVARGETGRQRRKRTEH"
     gene            2123..>2803
                     /locus_tag="VFY27_07840"
     CDS             2123..>2803
                     /locus_tag="VFY27_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5765464.1"
                     /translation="MKDILDKLGLGDVNPGTWSGSRSFEAASAPLIESVNPATNEVIA
                     SVRATTQEEYDQVVEAARAAFLEWRQVPAPVRGEAIRLLGDALRRHKNDLGSLVSLEN
                     GKIKAEGDGEVQEMIDIADFAVGQSRMLYGKTMHSERPMHRMYEQWHPLGIVGTISAF
                     NFPVAVWSWNACIAAICGNVNIWKPSPKTALCGVAVQKICNAALADAGFPEIFHLFND
                     GDNSLAQKF"
BASE COUNT          566 a          839 c          904 g          494 t
ORIGIN      
        1 cctgctcgta ataaagatgc aggccctcgt gcgaggaata gatgtcggcg cgctgcgtct
       61 ggtaaaacgc gcggccggag atggattcga tgaagtccag ggaattcgaa atcgagcgga
      121 caatctcgtg gtactcgtcc gcggtcttgg aatggccgac ccagtcgagg tcccagtact
      181 ccgggtgatg caggtcggcg aacccgccgt ctatcagcga gcgaacgaag ttgagcgtca
      241 gcgcggcacg ctcatagccg cgcaggatca ggtcggggtt cggcaccctg tccgccgcgc
      301 tgaaggggtt gcgattgacg agatcgccgc gaaagctcgg cagcgaggtc ccgtcgcggg
      361 tttcggtgtc cgcggagcgt ggcttggcat attggcctgc catgcggccg acgcggacca
      421 ccggcttttt catgccatac agcatcacca ggctcatttg caggaggatc tttaattttg
      481 cgacgatgct gtcggacgtg cagtcatcga aacgctcagc acagtcgccg ccctgcagga
      541 cgaacgcgtc gccgcgttgc gccttcgcga acaggtcttt gagggcgtcg acctcccagg
      601 aggtgaccag cgggggcagg cggctcagct ccgctacggt gcgctcgagc tccgccgggt
      661 ccggatagac gggctgctgg gcggcatcga gccgctgcca gctggcaggg tgccattcgg
      721 ttttcacgac aggtcgggtc acgggcgctc ccttggaagt ggcgcactat gccatgtagc
      781 ggaaaagggg cgcaaaggtt tcgggcgaaa tcaggatgtt gcgtggggaa ctgccgggtt
      841 cccgatcacg gcttgagatg cgtctggaaa gcgccgcgtg acattggcac aatgcgcgcc
      901 cgcggacttc ccgctttctc cccaggggca atcatgcagg acattctggt tctcggcgcc
      961 ggcaagatcg gggcgctgat tacgggtctt ttggccgagt gcggcgacta ccgcgtgcag
     1021 ctcgcggacg cggtggaagg cgctgcggcc ggcgttgccg aggcgcacgg gctggatccg
     1081 tcctgtgcct tcacgttcga cgccggtaac gaacagcaac tgacggaaca cgtgcgaaag
     1141 cacaagcctg tagccgtcat ctccggcctc cccttctatt gcaacgtcgc cgtagcgaaa
     1201 gtggcgcggg cggagaacct gcactatttc gatctcacgg aagacgtggc tgtgaccaat
     1261 tccgtgcgcg cgctggccaa aggggccaca acggcgttcg tcccccaatg cggactggcg
     1321 cccggtttca tcagcatcgc cgcgaacgag ctgatcaagc atttcgacaa gctgcgttcc
     1381 gtcaagctcc gggtcggcgc actcccacag cacccgaaca acgtcctgaa atactccctg
     1441 acctggtcga cggacggcct catcaatgaa tatggcaatc cttgccagag catcgtggaa
     1501 gggcaggaaa taaacgtgct gcccctcgaa gggctggagg aaatagagat cgatggaacg
     1561 ctgtacgaag cgttcaacac gtccggcggt cttggctccc tgggcgagac ctacggcgcc
     1621 cgcgtcaata cgatgaacta caagaccatc cgttaccccg gccactgcga gcagatgcgg
     1681 ctgctgatga acggcctgaa gctcaatcgc gaccgcgcaa cgctcaagcg gatcctcgag
     1741 aactccgtgc cgcagacact gcaggacgtg gtaatcatct acgccgccgt gacgggaatg
     1801 caggatggcg aattccgcga agagaactac gtcaacaagg tgtatccgga agtggtgagc
     1861 ggccggctct ggtccgcaat acagatcacg accgcagcgg gcgtgtgcag cgttatggat
     1921 atcgtgttgt cctcgcgccc gccgcggagg ggcttcatcg cgcaggagga gttcgggctg
     1981 caggaaatcc tcggcaaccg cttcggcgag tattatgaac atggcggcag caaccagctc
     2041 tctgcagacc tcgtcgcgcg aggcgagacc ggtcgccaac gcaggaagag gacagaacat
     2101 tgagtaaaca ggagcgatgc aaatgaagga catactcgac aaactcggac tcggcgacgt
     2161 caatcccggc acctggtccg gttcacgctc gttcgaagcg gcgtcggctc ccctgatcga
     2221 atcggtgaac cccgcaacga acgaggtaat cgccagcgtg cgcgccacga cgcaggaaga
     2281 gtacgaccag gttgtcgaag ctgcgcgtgc ggcatttctc gaatggcgcc aggtcccggc
     2341 cccggttcgc ggcgaggcaa tacgcctgct cggcgacgcg ctgcgcaggc acaagaacga
     2401 ccttggcagc ctcgtgagcc tggagaacgg caagatcaag gccgaaggcg acggcgaagt
     2461 gcaggagatg atcgatatcg ccgacttcgc ggtcggccag tcgcgcatgc tgtatggcaa
     2521 gacgatgcat tcggagcggc ccatgcaccg gatgtacgag cagtggcacc ccctcggtat
     2581 cgtcggcacg atttccgcat tcaacttccc ggtcgcggta tggtcgtgga acgcctgcat
     2641 cgcagcgatc tgcggcaacg tcaatatctg gaagccgtcg cccaagaccg cgttgtgcgg
     2701 cgtggccgtt cagaagatct gcaacgcggc tctcgccgat gcgggtttcc cggaaatctt
     2761 tcacctgttc aatgacggcg acaacagcct cgcgcagaag ttt
//
LOCUS       DASUOM010000376         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1207349,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000376 DASUOM010000000
VERSION     DASUOM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207349"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFY27_07845"
     CDS             complement(<1..1227)
                     /locus_tag="VFY27_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765465.1"
                     /translation="MIRKLLAIFAGLAIALAVVLAALLNWPLPDMPRPGVTGDFLVTN
                     VAIVDVMSSRIVPGRDVVVRDGRVDSIGASHPGSGQSSLVVVDGAGKFLAPGLWDMHV
                     HSLKISPQYIHPLFIATGVTGVREMWGCPSLPDSFVACREEIERWREGLRDRSHLAPR
                     YIQRSSFAINGEQGVPAAAPAFFKARNEDEARALVAHHAGDGVDLLKTYTNLSVAAYE
                     ALAAEAPKHGLFLAGHQPVRVSLQKVLAAGQHSIEHPRIFLFDCYRDVEAFRALPDPM
                     AAYTAGMRARLIDEHDPERCAELMSAMAASDTWWTPTLQVLRMSALAGNQEFREDPRR
                     RYIPFFIRSGIWGPDADRAAENAAHESGRDVDAELYQLALDNVRQAHTAGIRIVAGTD
                     AGDTYVFPGFAIHDELA"
     gene            complement(1224..1445)
                     /locus_tag="VFY27_07850"
     CDS             complement(1224..1445)
                     /locus_tag="VFY27_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765466.1"
                     /translation="MLSTLLNNLVANAFPYTGTGAIDIAWRGNALENRASGPAIVSII
                     KRLCGRCDVVLSIGPLENGGSRAEVKFPA"
     gene            1676..>2548
                     /locus_tag="VFY27_07855"
     CDS             1676..>2548
                     /locus_tag="VFY27_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX5765467.1"
                     /translation="MGLLATIGLALTLSACDRAPDPTAVTPAELRAIIKEAYIYGFPM
                     VDSYRIQHSYFVDEANPEYKGGWNEVHNVARVFTPLDKAIQTPNSDTPYSFLGADLRA
                     EPLVISVPAIHDRYYSLQFIDMFTHNFAYVGSRATGSDAGSYLLAGPGWQGQAPEGIK
                     SVIHSESDLAFVLYRTQLFGVDDLENVKRIQAQYEVQPLSRFLGTRPPPSRAVEFIEP
                     LSAEAQKTSPEFFGILNFLLQFTPTHPSEAALMDRFAKTGIGAGKSFDVSALSPEQRR
                     AVQEGMADAWKSFAQ"
BASE COUNT          496 a          777 c          784 g          491 t
ORIGIN      
        1 ggcaagttcg tcgtggatgg caaacccggg gaagacgtag gtgtcgccgg catcggttcc
       61 ggccactatc ctgatgccag ccgtgtgggc ctggcgcacg ttgtccagcg caagttgata
      121 caactccgcg tcaacgtcgc gaccggattc gtgcgcagca ttctccgccg cgcgatcggc
      181 atccggtccc cagatgcctg acctgatgaa gaacgggatg tagcgccggc gcggatcttc
      241 acggaactcc tggttgccgg cgagcgcgct catgcgtagc acctgcaggg tcggtgtcca
      301 ccaggtatcg gatgctgcca tcgctgacat gagctcggcg cagcgctcgg gatcatgctc
      361 gtcaattagt cgggcccgca tgccggcggt gtaggcagcc atcggatcgg gcaacgcgcg
      421 gaacgcctca acgtcccggt agcaatcgaa caggaaaatc cgcggatgct cgatgctgtg
      481 ctggccggcg gcgagtactt tttgaagcga aacacgcacg ggttgatgcc ccgccagaaa
      541 gagcccgtgc tttggcgctt ccgctgcgag tgcctcgtat gccgccacgg atagattggt
      601 gtaagtcttc agcagatcga caccatcacc ggcatgatgc gccaccagcg cccgggcttc
      661 gtcctcgttt cgcgccttga agaatgcagg tgcggcagcg ggcacgcctt gttcgccgtt
      721 gattgcgaaa ctgctgcgct ggatgtatcg gggcgcgagg tgactgcgat cacgcagccc
      781 ctcccgccag cgctcaattt cttcgcggca ggcaacgaag ctgtccggca gggatgggca
      841 gccccacatt tcacgcacgc cggtaacgcc tgtggcaatg aacagcggat ggatgtactg
      901 cggtgaaatc ttgagcgaat ggacatgcat gtcccacagg cccggggcca ggaacttccc
      961 ggctccatcc acaacgacga gactgctttg cccgcttccg gggtggctag cgcctatgga
     1021 atcaacccga ccgtcgcgga caacgacatc acgcccagga acgatccggc tgctcatgac
     1081 atcgactatg gcgacattcg tgacgaggaa atcgccggtt acgccgggac gcggcatatc
     1141 cggcaacggc cagttcagca atgctgccag cacgaccgca agcgcgatgg ccagccctgc
     1201 gaatatcgcc aataatttcc tgatcatgcc ggaaacttca cttcggcacg gctgccgcca
     1261 ttttcgagcg gaccgatgct gagaacgaca tcgcagcggc cgcacaaccg tttgatgatc
     1321 gacacgatgg ccggaccact ggcacgattc tccaatgcgt tgcctcgcca ggcaatgtcg
     1381 atcgcacccg tcccggtgta tgggaatgcg ttcgcgacaa ggttgttcaa aagggtggac
     1441 agcaccgatt gcggcgctgc cggccgccag atatggcgac tgccatgccg ctggtaaaat
     1501 atatcactca gtcaaaggac ttatgagtta ataacttaca caggcgaatc taacggcgct
     1561 ttgagacggt gtcgcaacgt catcgtttca acgtagccgg accgcatgta agttatcagc
     1621 agttccggcg actgcaggga tccgtaggat gacgacgacg cgtacggagt gtctcgtggg
     1681 tttgttggcg accatcgggt tggccttgac cctctccgcg tgcgacaggg ccccagatcc
     1741 gactgcggtg acgccggccg aactgcgagc gatcatcaag gaggcctaca tctacggctt
     1801 cccgatggtg gacagctacc gcatccagca ttcctatttt gtcgacgagg ccaacccgga
     1861 atataagggc gggtggaacg aggtgcacaa cgtggcccgc gtcttcacgc cgctggacaa
     1921 ggcgatccag acccccaact cggacacgcc ctacagcttt ctcggcgcgg atcttcgcgc
     1981 cgagccgctg gtgatcagcg tgccagcgat ccacgaccgc tattactcgc tgcagttcat
     2041 cgacatgttc acgcacaatt tcgcgtatgt gggtagccgt gcaaccggca gcgatgccgg
     2101 cagctacctg cttgctggcc ccgggtggca gggtcaggcg ccggaaggga tcaagtctgt
     2161 gattcattct gagtcggatc tggcgtttgt gctgtaccgc acccagctgt tcggcgttga
     2221 tgacctggag aacgtgaaga ggatccaggc gcaatacgag gtccagccgc tgtcacggtt
     2281 tctggggacc aggccgcccc cgtcgcgtgc cgtggagttc atcgagccgc tgtcggccga
     2341 ggcgcagaag acctcgccgg agtttttcgg cattctcaac ttcctgctcc agttcactcc
     2401 gacccatccg agcgaagcgg cattgatgga tcgcttcgcc aagaccggca tcggtgcggg
     2461 caagtcattc gatgtttctg cgctttcgcc tgagcagcgc cgcgcggtgc aggaggggat
     2521 ggccgatgcc tggaagtcct ttgcgcag
//
LOCUS       DASUOM010000377         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_280773,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000377 DASUOM010000000
VERSION     DASUOM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280773"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFY27_07860"
     CDS             complement(<1..432)
                     /locus_tag="VFY27_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765468.1"
                     /translation="MGWMRPYEAHNGESELDSRAPFFWRAVVASSLMVATAVLCSNAV
                     GARSDDIIWEGDDQSVILAPQDIPDAAPNEHPVTLQPQDIETMLSGLRFRHADQEAET
                     PPAAVFNKEQVAVLKDALAEGLSRATPSQDVTFSVVGAHRMA"
     gene            complement(447..1715)
                     /locus_tag="VFY27_07865"
     CDS             complement(447..1715)
                     /locus_tag="VFY27_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5765469.1"
                     /translation="MKNEAVGHDHTTTSIAVATLLLLSINGHAFESMQMGDWQVQFTG
                     NIDGFLTNTECDAGAGAVAGGLACGSNGVDRDLGNIRTGLLPSWFSFNAKQETDNIST
                     EIMIGFQPGIDGGNQGGITGSPLDGGLGLNSENLRQVFVQFGSDWGKFKIGRDLGLFG
                     SDAILSDMTLLGVGTVSDLTAGGGNTSLGRIGVGYMYADWKGQIQYSSPDWNGFTFNL
                     ALVDPWGLVNLSGLSLDAGTFAQQGDTYGIEGKVGYEFKGPEAEDLSGKIWASFINQS
                     LDSATFSSEDATGFDIGAKVGIAGFQVVAYYYDGEGIGTADFLFDAVDLAGNARDSDG
                     GYIQATYAIPGPGTKVGVSFGESNLDLGPADLATSSLVKTNESLVFGVYHPLTESLNL
                     VFEDTNTEATAHNGNQAEETSIAIGAIIFY"
     gene            complement(1981..4485)
                     /locus_tag="VFY27_07870"
     CDS             complement(1981..4485)
                     /locus_tag="VFY27_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765470.1"
                     /translation="MVRKILSTSKGAGAILLVAGAAFLPTASHAQQEDEITFDEIIVT
                     VTPVGGATGLRKEQIAYNIQSAFSDDIESMQPLHIGDFLNRTMGSISINDAQNNPLQP
                     DISYRGFTASPLLGLAQGIAVYQNGVRINEPLGDTVNWDLLPESAVHSLQLIGGANPL
                     FGLNTLGGALVVDMKDGFNSKGHHLELKGGSFGRAIVSAETGGNTDHFAWYGNVQYFD
                     EDGWRDASPSDAANFYGSLGWRNDLSSLDLNGQYGTSDLIGNGAIPVELLAIDRHAIF
                     TAPDITANKLYMLSLDGTHRVRDDITLSGNIFYRRNETDSFNGDASEFLVCQLGGTEA
                     LLEGLEGDDLEELDLEQEDVCGGQFANAADLETFLNNAATGMGEDPEFNIDDLTPELS
                     GTGDLSGSAINNSSTRIQRSYGSDVQMAFDRNLFARDNQLVVGAAWFNGESSFNSLLE
                     LANLDPATRSTAGLGTGTFVDEAATRIKTDTSTLSLYLTDTISLTEKLSFTLSGRFNR
                     TSIELADQSGERPELNGKHRYSRFNPAIGLTYQLNEEINLYGGYSESSRAPTPVELAC
                     NDHIFEIAVANAIAEGEDPEDVEFECRLPNAFLADPPLEQVVARSFELGARWNINDVD
                     VNLGLFHTTNNDDIIFQTTGRSTGLFANVDKTRRLGFESSLNGNWRNLDWFLAYSYIE
                     ATFEAPFDALSPNHPFADDEEGTIAVGPGDRIPGVPDHHLKLGGDHLFSDSLRIGIDL
                     LHSSGQRVRGDESGQLEKTNAYTVLNLRGHYRLNDHLAFFATVNNVLDEEYETFGLIG
                     EEPGEVDVPAFAEFDNPRFLGPAAPRAAFVGVKLSL"
     gene            complement(4491..>4917)
                     /locus_tag="VFY27_07875"
     CDS             complement(4491..>4917)
                     /locus_tag="VFY27_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5765471.1"
                     /translation="EWRFREIPFQLHKNIGITLVGLIIILLYVRFRHRPQPRLDQLLS
                     PRMSRLAVIDHVILYGLIIACCVSGYMSSSYSGWGTTLWWAVELPYWGHENEDLNIFW
                     SDIHLWTCWMLLAVVAVHISGALYHAFRNDSVIRRMLHL"
BASE COUNT          951 a         1467 c         1452 g         1047 t
ORIGIN      
        1 agccattcgg tgagcgccga cgaccgagaa cgtcacgtcc tgtgacggcg tcgcgcggga
       61 cagtccttcg gcgagcgcat ccttgagaac cgccacctgt tccttgttga ataccgcggc
      121 cggaggcgtc tccgcttcct ggtcggcatg cctgaagcgc aagcctgaaa gcatggtttc
      181 gatatcctgc ggctgcaggg tgacgggatg ctcgttgggt gcggcgtcgg gtatgtcctg
      241 gggcgccagg atgacggact gatcgtcgcc ctcccatatg atgtcatccg atcgcgcacc
      301 cacggcattg ctgcagagca ccgccgtcgc aaccataagc gaactcgcca cgaccgcgcg
      361 ccagaaaaat ggcgcgcggc tgtcaagttc gctctcgccg ttatgagctt cgtacggcct
      421 catccaaccc attgacgcga tcgatctcaa taaaaaatga tagcgccgat ggcgatgctg
      481 gtttcctcag cctggttgcc gttgtgcgca gtggcttccg tgttggtgtc ctcgaacacg
      541 agattcagcg attccgtcag cggatgatag acgccgaaga cgagcgactc gttggtcttc
      601 accagtgaag aagtagcgag atcggccgga cccaggtcga ggttgctttc gccgaagctc
      661 acgccgacct ttgtaccggg tcccggaatc gcgtacgttg cctggatgta gccgccgtca
      721 gagtcgcgtg cattgcccgc caggtccacg gcgtcgaaca ggaaatcagc ggtgccgatg
      781 ccttcgccgt cgtagtagta cgcaacgacc tggaagcctg cgatgcccac ctttgctccg
      841 atatcgaagc cggtggcgtc ctcgctgctg aaggtcgcgc tgtccagcga ctggttgatg
      901 aagctcgccc agatcttgcc gctcaagtcc tcggcttccg gccctttgaa ctcataccca
      961 accttgcctt cgatgccata ggtgtcgcct tgctgcgcga acgtgccggc gtcgagactc
     1021 aggcctgaca ggttcaccag gccccacgga tcgacaagag cgaggttgaa cgtaaagccg
     1081 ttccagtccg gtgaggaata ctggatctgc cctttccagt ctgcatacat gtagccaacg
     1141 ccgatacggc cgagcgatgt gttgccgccg ccggctgtca ggtcagagac agtaccgacg
     1201 ccaagcagcg tcatgtccga gagaatggcg tcggagccga aaagaccgag atcgcggccg
     1261 atcttgaatt ttccccagtc cgaaccgaac tgcacgaaga cctgccgcag gttctcggaa
     1321 ttcagtccca atccgccatc caggggactg ccggtgatac cgccttgatt gccgccatct
     1381 atgcccggct ggaaaccgat catgatctcg gtagagatat tgtcggtctc ctgtttcgcg
     1441 ttgaagctga accatgacgg caacaagcca gtccgaatgt taccgagatc gcggtctacg
     1501 ccattcgagc cgcaggcaag gccacctgca accgcaccgg cgcccgcatc acactccgtg
     1561 ttggtcagga atccgtcgat gttaccggtg aactggactt gccagtcacc catctgcatc
     1621 gactcgaaag catgtccatt gatcgacagc aacaagagcg tcgcaactgc gatcgatgtc
     1681 gtggtgtgat cgtgcccaac tgcttcgttc ttcatcttga tatcccccgt ttcagccgcc
     1741 agtacctgaa tctgtcaacc ggattactcg ccttcgccga aagggcgggg ctcgttacac
     1801 gcccttgccg cgatatttcg gtatggtccc tcgccaggcc tgcctggtac cgatgcgttg
     1861 acagccgcat cgccctacca tgggaaatca gcagtaacgc aataatgcgc ttcgaccccg
     1921 gcatgtcaat aacctgccgt caaataatta ctttttttac aagcgcttcc ccggtttcga
     1981 ctacaggctc agcttgaccc cgacgaacgc tgcgcgtgga gcggcgggcc ccaggaaacg
     2041 cggattatcg aattccgcga aggccggtac gtccacctca cctggttcct cgccgatcag
     2101 gccaaaggtt tcgtactcct cgtccagcac gttattgacg gtggcaaaaa acgcgaggtg
     2161 gtcgttcagc cggtaatggc cgcgcaggtt gagcacggta tacgcattcg tcttctcgag
     2221 ctggccggat tcatcgccgc gcacgcgctg cccgctgctg tgcagaaggt cgatgccgat
     2281 tcttaaactg tcgctgaaga ggtggtcgcc gccgagtttc aggtgatggt cggggacgcc
     2341 gggtatgcga tcccccgggc caacggcaat ggtgccttcc tcgtcatccg caaacgggtg
     2401 attggggctc agggcatcga acggcgcttc gaacgtggct tctatatagc tgtacgcaag
     2461 gaaccagtcg agattgcgcc agttgccgtt caagctgctc tcgaatccta ggcggcgggt
     2521 cttgtcgacg ttggcgaaca gcccggtcga tcgccccgtg gtctggaaaa taatgtcgtc
     2581 gttgttcgtc gtatggaaca gccccaggtt cacgtccaca tcgttgatgt tccagcgcgc
     2641 gccaagttcg aaactccggg cgacaacctg ctccagcgga ggatcggcca ggaaagcgtt
     2701 gggcaggcgg cactcgaact ccacgtcttc cggatcctca ccctccgcta tcgcgttggc
     2761 cactgcgatt tcgaagatat ggtcgttaca tgcgagctcg acgggcgttg gcgcgcgcga
     2821 ggactcgctg taaccgccat acaaattgat ctcctcgttg agctggtagg tcaggccgat
     2881 ggccgggttg aaccggctgt atcgatgctt gccgttcagc tcgggccgtt cgcccgactg
     2941 gtcggcaagc tcgatgctgg tgcggttgaa gcggccggac agggtaaagc tgagcttctc
     3001 tgtcaagctg atcgtgtcgg tcaggtacag gctcaaggtc gaggtgtcgg tcttgatgcg
     3061 cgttgcggct tcatcgacga aagtgcccgt acccaggccc gcagtgctgc gggttgcggg
     3121 atcgagatta gctagttcca ggagggagtt gaacgacgat tccccgttga accaggcagc
     3181 tcccacgacc agctggttgt ccctcgcgaa gaggttgcgg tcgaacgcca tttgcacgtc
     3241 tgagccatag cttcgctgta tgcgggtgct ggaattgttg atcgcggagc cggagagatc
     3301 accggtaccg gaaagctccg gtgtgaggtc gtcgatgttg aattcgggat cctcgcccat
     3361 gcctgtggcg gcattgttga gaaacgtttc aagatccgct gcattggcga actgcccgcc
     3421 gcacacgtct tcctgctcga ggtccagttc ctcgagatcg tcaccctcca ggccttccag
     3481 cagcgcttca gtccctccca gctggcacac gaggaactcg gacgcgtcgc cattgaacga
     3541 gtcagtttcg ttgcgccggt aaaagatgtt tccggaaaga gtaatgtcat cccgcacacg
     3601 atgagttccg tccaggctaa gcatgtacag cttgttcgcg gtgatgtccg gcgccgtgaa
     3661 gatggcgtgg cgatctattg ccagcagttc aaccgggatt gcgccattgc caatcaagtc
     3721 actcgtgccg tactgcccgt tcaggtccag ggaggacaag tcgttcctcc agccgaggct
     3781 gccgtagaaa tttgcggcgt cggacggcga cgcgtccctc cagccgtctt cgtcgaagta
     3841 ctgcacgttg ccgtaccagg cgaagtggtc ggtgttgccc ccggtttctg ccgagacgat
     3901 cgcccggcca aacgagccgc ccttcagttc caggtggtga ccttttgagt tgaagccgtc
     3961 cttcatgtcg accacgagcg ctccacccag ggtattgagg ccgaacagcg gattcgcgcc
     4021 accgatcagc tgcaggctgt gcacggcgga ttcggggagg aggtcccagt ttaccgtgtc
     4081 tcccaggggc tcgttgattc gtaccccgtt ctggtacacc gcaatgccct gggccagacc
     4141 gaggagcggt gatgcggtaa agccacgata gctgatgtcc ggctgcagag gattgttctg
     4201 cgcgtcgttg atggagatgc tgcccatcgt ccggttgagg aaatccccga tgtgcagggg
     4261 ctgcatgctc tcgatgtcgt cgctgaacgc ggactggatg ttgtaggcga tttgctcctt
     4321 ccgtagcccc gtggcaccgc cgacgggggt cactgtgacg ataatttcgt caaacgtgat
     4381 ctcgtcctcc tgctgtgcgt gcgaagccgt cgggaggaat gctgctcctg cgaccagcaa
     4441 tatcgcaccc gctcctttag atgtggaaag aattttacgc accattgaca ctacaaatgc
     4501 agcatccgcc gtatgacgct gtcgttccgg aaagcgtggt agagggcacc gctgatatgc
     4561 accgccacta cagccagcag catccagcac gtccacaaat gtatgtcgga ccagaagata
     4621 ttcagatcct cgttctcatg tccccaatag ggcagttcca ccgcccacca cagcgttgtg
     4681 ccccagccgc tgtaggatga ggacatgtac ccgctcacgc agcacgcaat gataagccca
     4741 taaagtatga cgtgatcgat gaccgcgagt ctgctcatcc ggggggacag gagctggtcc
     4801 agcctgggtt gtggtcgatg cctgaaccgg acgtagagga ggatgatgat cagtccgacc
     4861 agcgtgattc ctatgttctt gtgcagctgg aatgggattt cgcggaaccg ccattcc
//
LOCUS       DASUOM010000378         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1220351,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000378 DASUOM010000000
VERSION     DASUOM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220351"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1392)
                     /locus_tag="VFY27_07880"
     CDS             complement(97..1392)
                     /locus_tag="VFY27_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5765472.1"
                     /translation="MSVGQIFSSNALQAPTAKVGWYRWRICALLLLATTINYIDRQVL
                     GVLAPHLQSEIGWNEIEYGYIVTAFQAAYAIGLVTSGAIIDKLGTRIGYGLAISVWGI
                     AAMSHSLAATVLGFAVARFALGLGEAGNFPAAIKTVAEWFPRRERAFATGLFNSGSNI
                     GAIVAPLVVPIIAVTWGWRAAFLFTGFLSAAWLVTWLTTYRPPEQHPSLSKEELAYIR
                     SDPEEPTVKIPWKQLLRHRQTWAFAVAKFMTDPIWWFFLFWLPKFLYAEYGLTLTQLG
                     LPLVIIFLMADVGSILGGWLAGWFIRRGATVNRGRKSAMFICALAVVPIIFASQANNL
                     WLAVGLMGLATAGHQGWSANVFTLTSDMFPRRTVASVVGIGGFAGAVGGMMISTVTGF
                     LLETTGSYLPVFLMAGSAYLLALGVVHLIAPRLKPAAIR"
     gene            complement(1559..>2217)
                     /locus_tag="VFY27_07885"
     CDS             complement(1559..>2217)
                     /locus_tag="VFY27_07885"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765473.1"
                     /translation="SREMVLRLAEKLDVPLRERNELLVAAGFAPLFPERSLDDPTLQA
                     ARRAVDLVLKGHEPFPALAVDRHWTLVAANAAVPPMLEAVDSMLLEPPVNVLRLSLHP
                     GGLAPRIANMGEWRAHLFERLRRQIELTADLQLINLLEELQGYPVPGEKSAELPAVDY
                     GSVVVPFRLRTGDRELAFFSTTTVFGTPIDITLSELAIEAFFPADEATASAILASASG
                     "
BASE COUNT          428 a          710 c          712 g          367 t
ORIGIN      
        1 cgcaaggagt acctgcctcg tattcatccc gcttgctcct tgagcctcat gtttcgcggc
       61 ggaattgccc ccggatcgaa cgctgcacgt cgcgggtcac cggatggctg ccggcttcaa
      121 cctcggcgcg atcaggtgca cgacgccgag cgccagcagg taggcagacc ctgccatcag
      181 gaagacgggc aggtagctgc cggtggtctc cagcaggaag ccggtcacag tcgagatcat
      241 cattccaccg acagctcccg cgaaaccgcc gatgccgact actgacgcga ctgtgcgccg
      301 cgggaacata tccgaagtca gcgtgaacac gttggcggac caaccctggt gccccgcggt
      361 ggcaaggccc atgaggccga ccgccagcca taaattgttc gcctgcgagg cgaagatgat
      421 cggtaccacg gccagcgcgc aaatgaacat cgcactcttt cggccgcgat tgacggtggc
      481 gccgcgccga atgaaccacc cggccagcca gccgcctaag atgctcccga catccgccat
      541 gaggaaaatg atgacgagcg gcaagcccag ctgggtcaac gtaagcccgt actccgcgta
      601 caggaacttc ggcagccaga acaggaaaaa ccaccagatg ggatccgtca tgaactttgc
      661 cacggcgaac gcccatgtct ggcgatgccg caacagctgc ttccatggga tcttgaccgt
      721 gggttcttcc gggtcgctgc ggatataggc cagttcttcc ttcgacaacg aaggatgctg
      781 ttccggtggg cgataggtgg tgagccaggt gaccagccag gctgcgctca ggaaacccgt
      841 gaagagaaac gcggctcgcc atccccaggt cactgcgatg atcggcacga ccagcggcgc
      901 cacgatagca ccgatgttgg agccggagtt gaataggccg gtggcaaagg cgcgctcgcg
      961 tcgcgggaac cattcggcga cggtcttgat cgccgcgggg aagttgccgg cttcgcccag
     1021 gcccagcgca aaccgggcta ccgcgaagcc gagcacggtg gccgcgagcg agtggctcat
     1081 cgccgcgatg ccccagacgg aaatcgcaag cccgtaaccg atgcgggtac cgagtttgtc
     1141 gataatggcg cccgaggtaa cgagccctat cgcgtaagcc gcctgaaacg cggtgacgat
     1201 gtagccgtac tcgatctcgt tccagccgat ttccgattgc aggtgcggcg ccagcacgcc
     1261 gaggacctgc cggtcgatat agttgatcgt cgtggcaagc aacagcaggg cacagatgcg
     1321 ccagcggtac cagccgacct tcgcggttgg cgcctgcaag gcattcgagg agaatatctg
     1381 gccgacgctc atcgccgtgg ctgttgctgc atgccggtgc ccctgtcgcc cgttgtctca
     1441 ccgctttcac cggcggatta cgcattctct tgcgcattct ctgttaccgg tatcatgatc
     1501 gcaacggggg cccgccgcgg tcaacgcctg tcgggacccg cgatgctcct ggaagctgct
     1561 atccggaggc ggaggcaagg attgccgaag cggtcgcttc gtccgcggga aagaacgcct
     1621 cgatcgcaag ctccgacagg gtgatgtcga ttggcgtgcc gaacaccgtc gttgtgctga
     1681 agaatgccag ctcgcggtcg ccggtccgga gacggaaagg gaccacgacg ctgccgtagt
     1741 ccactgcggg cagttctgcg gacttctccc caggcaccgg gtaaccctgg agctcctcca
     1801 gcaaattgat cagttgcaaa tccgctgtca actcgatctg gcgccgcagg cgttcaaaaa
     1861 ggtgcgcgcg ccactcgccc atgttcgcga tccggggcgc gagtccaccg gggtgcaggc
     1921 tcaggcgcag cacgttgacg ggcggctcga gcagcatgct gtccacggct tccagcatcg
     1981 ggggtaccgc ggcgttggcg gccaccaggg tccagtgcct gtcgacggcg agcgcgggaa
     2041 acggctcatg gcccttgagc acgaggtcga cagcgcggcg ggcagcctgc agggtcgggt
     2101 cgtcgagcga ccgctccggg aacagcggag cgaacccggc agcgaccagt aattcattcc
     2161 gctcgcgcag cgggacgtcg agcttctctg ccagccgcaa taccatctcg cggctcg
//
LOCUS       DASUOM010000379         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_501615,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000379 DASUOM010000000
VERSION     DASUOM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501615"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1037)
                     /locus_tag="VFY27_07890"
     CDS             complement(153..1037)
                     /locus_tag="VFY27_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765474.1"
                     /translation="MKRKRCAFLAMENTAGWFIDSDLAIPSMQALLWTIDTVPWRGAG
                     TDWHRYDAVYIGTPWDYPEDVDRFMRLLEQIDASGPVLINPLELVRWGLAKTYLRDLE
                     AKGADIVPSAWYEQLSPGQLHSCFDELGSDRIVIKPVVSTNATDTFLLESHEASRLEP
                     LLQQRFENRAFIAQPFIRNVQVEGEFSLFYFGGVYSHAIQKVPRAQDFRVQEEHGASI
                     LAIEPEPALAACAGKILQLVDPLPVYARCDLVRGPDGLFLLMELELIEPSMYLRMNAD
                     APQRFAQAFDRYVREKKP"
     gene            complement(1058..1588)
                     /locus_tag="VFY27_07895"
     CDS             complement(1058..1588)
                     /locus_tag="VFY27_07895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765475.1"
                     /translation="MPEPYSIVAAAKQHLPEIPKLEQAAASVFPEEDLPAALRWLVTD
                     RDTLLAAQLEGRLWVAVNEGKQPVGFALAEVLDGEAFLDEVDVHPRHARRGIGTRLVN
                     TVIGWAENREYSSLSLITFKHLPWNARFYERFGFVPLTRAEMSPQMQQILDEEARAGI
                     DVSKRVGMRRVLQRPH"
     gene            complement(1622..2293)
                     /locus_tag="VFY27_07900"
     CDS             complement(1622..2293)
                     /locus_tag="VFY27_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX5765476.1"
                     /translation="MNVERSIVAGDPSRLMQAAWASVFAAFFVWSAIGPKDYPTWFVE
                     VLPAVVGAGILWFTRKRYPLTSLAYVLILIHCVVLIVGGHYTYAEVPLFDWVRDTFGQ
                     DRNNYDKLGHFLQGFVPAIIARELTIRNAVFSSVAWRNFFIVAACLGISAFYELIEWM
                     AALVSAEASESFLGTQGYVWDTQSDMAWALSGAILALLLLGRLHDRQLSERRWRRPLR
                     PPSLS"
     gene            complement(2323..2529)
                     /locus_tag="VFY27_07905"
     CDS             complement(2323..2529)
                     /locus_tag="VFY27_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX5765477.1"
                     /translation="MNEQRLIDIEVKLAHHEQALEEVNQVLTDQHARLSRLERVCESL
                     VEKFRALSEAPARDERDEPRPPHY"
BASE COUNT          539 a          854 c          820 g          513 t
ORIGIN      
        1 aagacccgca ccgtccggtt cgccggctaa cgccgataag cggaagagaa tgcaacaaaa
       61 tcgcgcgcgc cgcaaagaag agtctgcgat ggcgcgatgg gtcaggggaa tatgcacgcg
      121 atccgcgcgg cagggacccg ccatgggggc tcctacggtt tcttttcccg gacgtagcga
      181 tcgaacgctt gtgcgaagcg ttgcggggcg tcggcgttca tgcgcaggta catcgacggc
      241 tcgatgagct ccagctccat cagcaggaag aggccgtcgg ggccccgcac aaggtcgcag
      301 cgcgcataga cgggcagcgg gtcgacgagc tgcagtatct tgccggcgca agcggccagg
      361 gccggctccg gctcgattgc aaggatgctc gctccgtgct cttcctgcac ccgaaagtcc
      421 tgcgcgcgcg gcactttctg gatcgcgtgg ctgtagacgc cgccgaagta gaacagtgag
      481 aactccccct ccacctgtac gttccggatg aagggctgcg caatgaatgc ccgattctcg
      541 aagcgctgct gaaggagcgg ctcgagccgg gacgcctcgt ggctctcgag caggaacgta
      601 tcggtggcat tggtgcttac cacgggcttg atgacgatgc ggtcacttcc gagctcatcg
      661 aagcatgaat gaagctgccc gggtgacagt tgctcgtacc aggcgcttgg aacgatatct
      721 gcccccttcg cttccaggtc gcgcaagtag gtcttggcga ggccccagcg caccagctcg
      781 agagggttta tgagtaccgg cccggacgcg tcgatctgct cgagaagccg catgaatctg
      841 tccacatcct ccgggtaatc ccagggagtt ccgatgtaga cggcgtcata acggtgccag
      901 tcggtgcccg ctccgcgcca gggaacggta tcgatcgtcc acaaaagcgc ctgcatcgat
      961 ggaatggcaa ggtccgaatc gatgaaccag cctgcggtgt tttccatggc gaggaacgcg
     1021 cagcgcttcc tcttcatgga ttcatgtcag ccgttcgtta atgagggcgc tgcagcacgc
     1081 gccgcatgcc gacccgcttg gagacgtcga tgcccgcccg cgcttcctca tcgaggattt
     1141 gctgcatctg ggggctcatt tcagcccttg tgagcggtac gaagccgaac cgctcataaa
     1201 agcgcgcgtt ccagggaagg tgtttgaacg tgatcagcga caagctgctg tactccctgt
     1261 tctccgccca cccgatgacc gtgttgacga ggcgtgtgcc gatacccctg cgcgcgtggc
     1321 gcgggtgcac gtccacttcg tcgaggaatg cctctccgtc cagcacttcc gcaagtgcga
     1381 atcctaccgg ctgcttacct tcgttcacgg ccacccacag ccgcccttcc agctgcgccg
     1441 ccagcagggt atcgcggtcg gtaacgagcc agcgcaacgc tgccggcaga tcctcttccg
     1501 ggaaaaccga tgcggcagct tgctccagtt tcggtatttc aggcaaatgc tgcttcgcgg
     1561 cggcaacgat agagtaaggt tcaggcacgg gacagaaatg gggaaacgcg cgctcggtgg
     1621 ctcaggaaag tgacggaggc cggaggggcc tgcgccacct cctctcgctt aactggcgat
     1681 cgtgcaggcg accgagcagg agcagggcca atatcgcgcc ggataacgcc catgccatgt
     1741 ccgactgcgt atcccagaca tacccctggg tgccgaggaa cgactccgac gcttccgcgg
     1801 acaccagtgc ggccatccac tcgatcagtt catagaacgc gcttataccc aggcatgccg
     1861 ccacgatgaa gaagttacgc cacgccacgc tggagaagac ggcattcctg atcgtgagtt
     1921 cgcgagcgat gatcgcgggt acgaatccct ggagaaagtg cccgagcttg tcgtagttgt
     1981 tgcggtcctg tccgaacgta tcccgcaccc agtcgaacag cggcacttcg gcatacgtat
     2041 agtgaccgcc cacgatcagc acgacgcagt ggatgagtat gagaacgtag gccagcgatg
     2101 tcagcgggta gcgcttcctg gtaaaccaca ggatgcccgc cccgacgacg gctggcagca
     2161 cttcaacgaa ccaggtcgga taatcctttg gcccaatggc ggaccagaca aagaatgccg
     2221 caaacaccga agcccacgca gcttgcatga ggcgcgacgg gtcccccgcc acgatacttc
     2281 gttcgacgtt catcacgctc tcctggcttc gcatacttca gctcaatagt gaggcggcct
     2341 gggttcgtca cgctcgtccc gcgccggcgc ttcagaaagc gcgcgaaatt tctcgaccag
     2401 cgattcacag acccgctcga gccgcgacaa ccgagcgtgc tggtccgtga ggacctggtt
     2461 gacttcctcc agcgcctgct cgtggtgggc gagcttcacc tcgatgtcga tcagcctctg
     2521 ctcattcatg ccagttccca actcgtataa gcggtagtaa tcagcacatt acgtacgtcg
     2581 gtcggaatct gcaataaagc tgcagtcgtg cctgccggcg ggacttgagc actgctccac
     2641 ggtgtatgct tgcgcgctcg atacctgacg tgccagccac cgtttatgac ccttattcgc
     2701 ttcgaccagg tctccctcga cttcgg
//
LOCUS       DASUOM010000380         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1342869,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000380 DASUOM010000000
VERSION     DASUOM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342869"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1173
                     /gene="ada"
                     /locus_tag="VFY27_07910"
     CDS             109..1173
                     /gene="ada"
                     /locus_tag="VFY27_07910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX5765478.1"
                     /translation="MSTSDLEPATADSRWDAIISRDPAADGSFFYGVQTTGVYCRPTC
                     PSKRPRRENVEFFATGKAAEEAGFRPCKRCSPRQAAQPVRHARKVAEACRLIEQSEQA
                     PDLNTLAKNAGLSPYHFHRIFKAATGLTPKAYATAHRARRVRSELARSSSVTEAIYDS
                     GYNSNARFYEKASDVLGMTPRAYRAGGAAASIRFAVGECSLGSILVAKSERGVCAILL
                     GDDPDMLARELQDQFPKANLIGGDSEFEQLVAKVVGFVESPRIGLDLPLDVRGTAFQQ
                     RVWEALRRIPSGATVSYSEIAREIGAPHAARAVAQACASNRLAVAIPCHRVVRNDGEL
                     SGYRWGVERKRELLAREAAR"
     gene            1170..1916
                     /locus_tag="VFY27_07915"
     CDS             1170..1916
                     /locus_tag="VFY27_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5765479.1"
                     /translation="MSRLASLEPVRPPPGMEARVGALDWDRLNDELYADGACVVGGLL
                     TEDECDHAAALYDDEPLFRSKVIMARHGYGRGEYKYFRYPLPSIVAGLRTHVWPKLAP
                     VANRWNEAMRIDVRYPASHAEFVRRCHDAGQVRPTPLMLNYAAGDYNCLHQDLYGEHV
                     FPLQLAILLSQPGNDFSGGEFVLTEQRPRRQSRPQVLPLVKGDAAIFAVHHRPVHGIR
                     GWYRVNMRHGVSRITAGHRRTLGIIFHDAR"
     gene            complement(2112..3131)
                     /locus_tag="VFY27_07920"
     CDS             complement(2112..3131)
                     /locus_tag="VFY27_07920"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5765480.1"
                     /translation="MAGSCGAPFDVRVAHDLRLCSRRSTNGQNVYTDAFQAFPQVREE
                     RAVVADDILNACCERRPQNAVRTSSFTVSSSPGEPMVQVPETTALLNLSGRVAMVTGA
                     SQGIGACIARRLAEAGASVVVHFRSKAREAASVAASIRDAGGNALCVQADLVDAAAAG
                     DIVQQAVGHFGGLDVLVNNAGKFPNQSLLDMTQDEWRGMYASVVESTMLCTQAAALHM
                     QLAGGAIVNIASIGALHPGPDHSHYNSAKAAVVMFTRSAALELGGYGIRVNAVSPGLI
                     ARPGIETEWPGGVERWKAKALLGRLGQPEDVADACLFLASPAARWITGHNLVVDGGVT
                     ASAIY"
     gene            complement(3145..3453)
                     /locus_tag="VFY27_07925"
     CDS             complement(3145..3453)
                     /locus_tag="VFY27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX5765481.1"
                     /translation="MLELRPTCENCNAPLPPDSLDARICTYECTFCASCVDNLLHNVC
                     PNCGGGFVPRPIRPSTNWKDSNYLGANPGSTMVKHRPVDPEAHGRFAAHIRSIPPHKR
                     "
BASE COUNT          688 a         1212 c         1108 g          648 t
ORIGIN      
        1 tcgtaaaaat aaaatccccc ggattcgacc gcaagaaccg gatattcgcc ctcctccgcc
       61 ttcgatagat tgcgtgcaag tcgaattcag gaggatcagg atatgtacat gagcaccagc
      121 gatctggaac ccgcaacagc cgattcacgc tgggacgcga tcatctcgcg cgaccctgcg
      181 gccgatggca gctttttcta cggcgtccag accaccggcg tctactgccg gcccacctgc
      241 ccgtcgaagc ggccgcgacg cgagaacgtc gagtttttcg ccacgggcaa ggcagccgag
      301 gaagcaggct ttcgcccctg caaacgctgc agcccccggc aggccgcgca acccgtccgt
      361 catgcccgga aagttgctga agcgtgcagg ctgatcgagc agtcggagca ggcgccggac
      421 ctcaacaccc tggcgaagaa cgccgggttg agcccgtacc attttcaccg catcttcaag
      481 gcggcaacgg ggcttacgcc taaagcgtac gcaaccgcgc atcgcgcccg gcgcgtgcgc
      541 agtgagcttg cccgctcgag ttcagtaacc gaggcgatct acgactccgg atacaactcc
      601 aacgcgcgct tctacgaaaa ggccagcgac gtactcggaa tgacacctcg cgcttatcgc
      661 gccggcggcg cggcagccag cattcgcttc gcggtcgggg agtgctcgct gggttccatc
      721 ctggtagcga aaagcgaacg cggcgtttgc gccattcttc tcggcgacga ccccgacatg
      781 ctggcgcgtg agctgcagga ccagtttccg aaggcgaacc tgatcggtgg agacagtgaa
      841 ttcgagcagc tcgtcgctaa agtcgtcggc ttcgtcgaat ccccacggat cggcctggac
      901 ctgccgctgg acgtccgcgg cacggcattc cagcaacgtg tctgggaggc gctgcggcga
      961 atcccgtccg gcgccacggt cagttactcc gagatagcca gggagatcgg cgcaccgcat
     1021 gccgcgcgag cggtcgcgca ggcttgcgca tcgaaccggc tcgccgttgc gatcccctgt
     1081 catcgcgtcg tcaggaatga cggcgaactc tcaggatatc gctggggcgt tgaacggaag
     1141 cgcgaactgc tcgcgcgcga ggcggcacga tgagccgact cgcgagcctc gaaccagtac
     1201 gaccaccgcc gggcatggaa gcccgcgtcg gcgcgctgga ttgggatcgt ctcaacgacg
     1261 agctttacgc ggatggcgcc tgcgttgttg gaggactgtt gactgaggat gagtgcgacc
     1321 atgccgccgc actctacgac gatgagccgc tttttcgcag caaggtcatc atggctcgcc
     1381 atggttacgg ccggggcgaa tacaagtact tccgttatcc gctgccctct atagtggccg
     1441 ggttgcgaac gcatgtgtgg cccaaacttg cgcccgtcgc caaccgctgg aacgaggcga
     1501 tgcgcatcga cgtgcggtat ccggcgtcac acgccgagtt cgtccggcgc tgccacgatg
     1561 ccgggcaggt gcggccgacg ccgctgatgc tcaactacgc cgctggtgac tacaattgcc
     1621 tgcaccagga tttgtacggc gagcatgtgt ttccactgca gctggcaatc ctgctttcgc
     1681 agccggggaa cgacttcagt ggcggcgagt tcgtgctgac cgagcagcgg ccacgccggc
     1741 agtcacgccc gcaggttttg cccctggtga aaggcgatgc ggcgatcttc gcggtgcacc
     1801 accggccggt gcacggcatc cgcggctggt atcgcgtcaa catgcgccac ggcgtcagcc
     1861 gcatcaccgc aggccaccgc cgcaccctcg gcataatttt ccacgacgcc cggtagcgac
     1921 ccgggcgacg aaacgggtgc ttgtgaatcg ggcgctgtcg gagcccgtcc tacggacgac
     1981 aacccgcagc gcaaaccgta acgaaccgga tagcctcgaa tagccgctgt aggagcccgc
     2041 catgcgggcg agttcgctga cgcttatcgc actcgcccgc gtggcgggct cctacgccga
     2101 ctcagccggc atcagtagat cgccgacgcc gtcacaccgc catccaccac caggttgtgc
     2161 ccggtgatcc accgcgcggc cggcgatgcg agaaacaggc acgcgtccgc cacgtcttcc
     2221 ggttgtccaa gccgcccgag cagcgccttc gccttccatc gctccacccc gccgggccac
     2281 tccgtttcga taccgggccg cgcaatcagc cctggggaaa ctgcgttcac cctgattccg
     2341 taaccgccaa gctcgagcgc cgcggagcgg gtgaacatca ccaccgcggc tttggcgctg
     2401 ttgtaatgac tgtgatccgg gcctggatgt aacgccccta tcgaagcaat gttcacaata
     2461 gcgccgcccg ccaattgcat atgaagcgct gcggcctgcg tacagagcat cgtggattcg
     2521 acgactgatg catacatccc gcgccactcg tcctgcgtca tgtcgagcag cgactggttc
     2581 gggaacttgc cggcgttgtt cacgagcaca tcaagcccgc cgaaatgacc gaccgcctgc
     2641 tgcacgatgt ctcctgctgc ggcagcatcg accaggtctg cctggacaca aagggcattc
     2701 cctcccgcat ctcgaatgga cgcagcaacc gaggccgctt ccctcgcctt gctccgaaaa
     2761 tgaactacaa cggaagcccc cgcctccgcc aaacgccgcg caatgcacgc accgatcccc
     2821 tggctcgcgc cggtgaccat tgcaacccgg cccgacagat tcaacaatgc ggtggtttcc
     2881 ggcacctgca ccataggttc tcctggtgaa gacgaaactg tgaacgatga cgtgcgtacc
     2941 gcgttttggg gacgcctctc gcagcaagcg ttgaggatat catcggcgac taccgctcgt
     3001 tcttcgcgga cctgcggaaa cgcctggaag gcgtcggtat agacgttctg gccgttcgtt
     3061 gagcgtctcg aacaaagtcg caagtcgtgc gccacgcgca cgtcgaaagg agcaccgcag
     3121 gagcctgcca tgcaggcgag tgcttcagcg tttgtgtggc gggatcgagc ggatatgggc
     3181 ggcgaagcgc ccgtgagctt caggatccac cggccggtgc ttcaccatgg tactgcccgg
     3241 attcgcgccg aggtagttac tgtctttcca gttcgtcgac ggacgtatag gcctcggcac
     3301 gaagcctccg ccacagttgg ggcacacgtt gtgcagcagg ttatcgacac aggatgcaca
     3361 gaacgtgcac tcgtaggtac agatacgcgc gtcaagcgaa tccggcggca gcggcgcgtt
     3421 gcagttctca caggtgggcc tcagttccag cacttctcct ccttttgttt cctcgcttac
     3481 gtacggtggc tccgacgaca gaacaagcgc cgcaggagcc cgccacgcgg gcgagttcgt
     3541 ccagtcgtat cgcactcgcc cgcatggcgg gctcctacag cgctttattc gggaccgcca
     3601 ttgcgggtcg agttcgctga ggctaatcac actcaaccgc gtagcgggct tccata
//
LOCUS       DASUOM010000381         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_641465,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000381 DASUOM010000000
VERSION     DASUOM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641465"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /locus_tag="VFY27_07930"
     CDS             <1..1945
                     /locus_tag="VFY27_07930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5765482.1"
                     /translation="LTWFAVTALATACSSGDKEPAPMAEAGREAKAPTQYDAEAFFAT
                     TTYSLAGGYAWSPDDGKLLASSDESGIFNAIALAVADGAKQPLTTSTTDSVFAVSWFP
                     DDDRILYTADKGGNEINHLFVREASGETRDLTPGDEVKAQFAGWSRDRKSFYALTNER
                     DPASFDLYRYAIDGYERTLVFQNDEAFDVSAVSPDGSYVALVKPRTSADSDIYLLHTL
                     EPDGKPALISQHTGNVTHSVYDFTRDSEKLVYSTDEHGEFTQAWTYDLNDGTRAPLIA
                     ADWDVMFVTFSESGRYRVSATNEDARTVVQILDSQSGDEISLPGLPAGDLAQIRFSRD
                     ESKVALLLSSDTSPSDVYTVDLASGTSARLTKALNPQIDEADLVETEVVRYKSFDGVE
                     IPGILYRPHTASATEKVPALVWVHGGPGGQSRTGYSATIQHLVNHGYAVLAANNRGSS
                     GYGKTFFHMDDRRHGDVDLQDIVLAKDYLASLEWVDGERIGIIGGSYGGYMVGAALAF
                     EPDVFDVGINIFGVMNWVRTLTSIPPWWESFKEALYDEMGDPATDGERHRRISPLFHA
                     QNIKAPLLVVQGANDPRVLQVESDEIVAAVRENDVPVEYVLFPDEGHGFTKRENRISA
                     SEAYLSFLDTHLKGDAGTPAGSR"
BASE COUNT          421 a          682 c          635 g          383 t
ORIGIN      
        1 tctcacatgg ttcgccgtca ctgcgcttgc caccgcatgc agttccggcg acaaggaacc
       61 cgcaccaatg gccgaagccg gacgcgaagc gaaagctccc acccagtacg acgccgaggc
      121 gtttttcgcc accacgacct actcgctcgc gggcggttac gcgtggtctc cggatgacgg
      181 caagttgctc gccagctcgg acgaaagcgg catcttcaac gccatcgctt tggcggttgc
      241 cgatggcgca aagcaacccc ttaccacgtc gacgactgac tccgtgttcg ccgtctcctg
      301 gtttcccgac gacgaccgga tcctgtatac ggccgacaag ggcggtaacg agatcaacca
      361 cctgttcgtc cgggaggcat cgggcgagac acgggacctc acacccgggg atgaagtaaa
      421 ggcgcagttc gcaggctggt ccagggaccg gaagtcgttc tatgcgctca ccaacgagcg
      481 cgacccggcg agcttcgatc tgtaccggta cgcgatcgac ggctacgaac gcacgctggt
      541 gttccagaac gatgaggcgt tcgacgtttc cgcggtctct ccggacggca gctatgtcgc
      601 gctcgtcaag ccgcgcacca gcgccgattc cgacatttac ctgctccaca cgttggaacc
      661 cgacggcaaa ccggcgctta tcagccagca taccggcaac gtgacgcatt ccgtctacga
      721 ttttacccgc gacagcgaaa agctggtcta ttccactgac gagcatggcg agtttacgca
      781 ggcgtggacc tacgacctca acgacggaac aagagcaccg ctgatcgccg cggactggga
      841 cgtcatgttc gtcacctttt ccgagtcggg ccgctaccgg gtttccgcca cgaacgagga
      901 tgcgcgtacg gttgtgcaaa tcctcgacag ccagagcgga gacgaaatct cgttgccggg
      961 acttccggcc ggagacctgg cacagatcag gttctcgcgc gatgagagca aggttgcctt
     1021 gctgctgagt tccgacacct cccccagcga cgtctatacc gtcgaccttg cctcaggaac
     1081 gagcgcgcgg ctcacgaagg ccctgaaccc gcagatcgac gaagccgacc tcgtcgagac
     1141 ggaggtggtg cgctacaaga gtttcgatgg cgtggagatt ccgggcatcc tgtaccgacc
     1201 gcataccgcg tcggcaacag aaaaggtacc tgcgctggtc tgggtgcacg gcggcccggg
     1261 cggccagagc cgtaccggtt attccgcgac catccagcac ctcgtgaacc acggctacgc
     1321 cgttctcgcc gccaacaacc gcggctcctc cgggtacggc aaaacgttct tccacatgga
     1381 tgaccggcgc cacggcgacg tggatctcca ggacatcgtc ctggcgaaag actatctcgc
     1441 gtcactggaa tgggtggatg gcgagcgtat cggcatcatc ggcggctcgt acggcggcta
     1501 catggtcggc gcagcgctcg cgttcgagcc cgacgtattc gacgtcggca tcaacatctt
     1561 cggcgtaatg aactgggtcc gcacgctgac cagcattccg ccgtggtggg agtccttcaa
     1621 ggaagcgctc tacgacgaga tgggcgaccc ggccaccgac ggcgaacggc accgcaggat
     1681 ctcgccgctg ttccacgcgc agaacatcaa ggcgccgctg ctcgtagtgc agggcgccaa
     1741 cgacccccgc gtgctgcagg tcgaaagcga cgagatcgtc gccgccgttc gcgaaaatga
     1801 cgtgccggtg gaatacgtcc tgttccccga cgaaggccac gggttcacca agcgtgagaa
     1861 caggatctcg gcttcggagg cctacctctc gttcctcgac actcacctga agggcgacgc
     1921 gggaaccccc gcgggttcca ggtagccgct cgacctgtca gaggtgcgat tttatgggca
     1981 cgaggacgtt ttccgcgacc ttctggcgca gcgaacgttt tttccacatc gagtaggtgt
     2041 attcgacagt tggctcgagg tctgcctcga aaacttccgt aagctgttct gcaaaatcct
     2101 tgtcgtagat attcaggctg g
//
LOCUS       DASUOM010000382         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_529604,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000382 DASUOM010000000
VERSION     DASUOM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529604"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="gspL"
                     /locus_tag="VFY27_07935"
     CDS             complement(<1..794)
                     /gene="gspL"
                     /locus_tag="VFY27_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX5765483.1"
                     /translation="MEYLVIRIGESAGDTVRWMAVDASGARRSAPATGMLHEAAADAG
                     ERKVIVLVPGTEVLTTTVDIPVRGAKLQAALPYALEEFLAEDVEKLHFAAGARRSSGR
                     VPVAVVSKEKLAGWLSHLNAAGIWPTSVIAENHGLARIPGTISLLIAENRILINDGGD
                     NELVMQDVSPGDALAAIGALDDGAGGGADTAGDVAPLPRHLLVYCEPGIDERYELDWM
                     AVDPSGARRSAPATGLLHEAAADVGERKVIVLVPGTEVLTTTVDIPV"
     gene            complement(799..1737)
                     /gene="gspK"
                     /locus_tag="VFY27_07940"
     CDS             complement(799..1737)
                     /gene="gspK"
                     /locus_tag="VFY27_07940"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX5765484.1"
                     /translation="MNARMVVRNRRNRGVALITAMLITALAAIVAYNLAWDNTLDVRR
                     TMVLLNHDQAVQVALGAESWVINILHQDMEDSQTDHLAEIWATELPGLPIDGGEVFGH
                     VEDLQGRFNINNLIDEGGAVDEEAVEQFRRLLVALELDPRFAGIASDWLDADLDAQFP
                     DGAEDPIYTGMVPPYRSANQSITSISELAALEGMDKVTFDILAPHITALPGRTTINVN
                     TATPQVLQSLDENISVTAVEALLAEREEAGFADIQTAFSSLVTPDVLNRLDEATNYFQ
                     LRVIVRIDTVRVTMYSVLLRSQRGDVTPILRSFRTI"
     gene            complement(1734..2348)
                     /gene="gspJ"
                     /locus_tag="VFY27_07945"
     CDS             complement(1734..2348)
                     /gene="gspJ"
                     /locus_tag="VFY27_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX5765485.1"
                     /translation="MSRPQQSGFTLVEVLVALAIFGMLAAIAYGTLGQTLANAEILTD
                     RMQRLQSLQRTVRYLSEDFMQLAPRPVREDLGENFGPALHTDVESDFAVELTHGGWSN
                     PATLPRGTQQRVAYRLEEGELIRYHWTVLDRTLSNEPAGRVLLDEVDSILFRFMQDNG
                     DWSEEWPPENRPGLLGLRQRPRAVEIILVLADGTEIRRLLEVSP"
     gene            complement(2345..2788)
                     /gene="gspI"
                     /locus_tag="VFY27_07950"
     CDS             complement(2345..2788)
                     /gene="gspI"
                     /locus_tag="VFY27_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX5765486.1"
                     /translation="MNRRRSDRRRSAGFTLLEVMVALGIAALSLTAVTAAMSQMVDAA
                     SSMRERTYASWIAQNKIAEMRLSNVVPDVSEDSGEVEFAGLEWTWRSTVSETGVENLF
                     RVDVAVSFVDSEAIIRSVTGFIGEPGIPGQSNIAWTTNLPAGEER"
     gene            complement(2769..3338)
                     /gene="gspH"
                     /locus_tag="VFY27_07955"
     CDS             complement(2769..3338)
                     /gene="gspH"
                     /locus_tag="VFY27_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HEX5765487.1"
                     /translation="MRPAVRKRGGFTLIEVLVVVLIMAIISAMLLMSFGILGDDRALQ
                     QQARRISSLIELATDEAIMQGRDFGLEFMQHGYRFVEHDPALDQWLEVVGDELLRPRP
                     LQEGMEFELFLEDRPVSLAEEAADTDVGEDSGDRERNSYLPHVLILSSGEVTPFHLRL
                     VRWADRSEVRMEMTLNGEFEINADESATL"
     gene            complement(3345..3788)
                     /gene="gspG"
                     /locus_tag="VFY27_07960"
     CDS             complement(3345..3788)
                     /gene="gspG"
                     /locus_tag="VFY27_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX5765488.1"
                     /translation="MHKTGVRNRRQRGFTLIEIMVVVIILGILAAIVAPNVIGRIDDA
                     QITRVQSDLRGIESALNFYRMDNFSYPTSEQGLEALVTKPADPNIRNWKAGGYLDRAP
                     KDPWGNPYLYLNPGNHGEIDVYTLGRDGRPGGEGLDADIGNWELE"
BASE COUNT          684 a         1249 c         1190 g          786 t
ORIGIN      
        1 accggtatgt cgacggtggt ggtgagaacc tcggtgccgg ggacgaggac gatcaccttg
       61 cgttcgccga catccgcggc ggcctcgtgc agcaggccgg tcgccggggc actgcggcgt
      121 gcgccgctgg gatccaccgc catccagtcg agctcgtagc gctcgtcaat tcccggctcg
      181 cagtaaacga gaaggtggcg gggcagggga gcaacatccc cggcagtatc tgcgccgccg
      241 ccggcaccgt cgtccagagc gccgatggcg gcgagagcgt cacccgggct gacgtcctgc
      301 atcacgagtt cgttatcgcc gccgtcgttg atcaggatcc tgttctcggc gatcaggagg
      361 cttatggttc cgggtatgcg cgcgaggcca tggttctcgg cgattaccga cgtgggccag
      421 attccggcgg cgttcaggtg cgacaaccag cccgcaagtt tttccttgct gacgaccgca
      481 accggtactc ttccgctgct gcgacgggcg ccagcggcga aatgaagctt ctcgacatcc
      541 tcggccagga actcctcgag tgcatacggc aatgccgcct gcagcttcgc tcctcgtaca
      601 ggaatgtcga cggtggtggt gagaacctca gtgccgggga cgaggacgat caccttgcgt
      661 tcgcctgcat cggcggcggc ctcgtgcagc atgccggtcg ccggcgcact gcggcgcgcg
      721 ccgctggcat ccaccgccat ccagcgcacc gtgtcccccg cgctttctcc gatccggatt
      781 accaggtatt ccatcacact aaatcgtccg gaagcttctg aggatagggg tcacgtcgcc
      841 gcgctggctg cgcaacagga cgctgtacat cgtgactcgt acggtgtcta tgcgcacgat
      901 gacacgtaat tggaagtagt ttgtcgcctc atccagcctg tttagcacat ccggagtcac
      961 cagcgatgag aatgccgtct gaatatcggc aaatccagcc tcttcgcgct ccgcaagcag
     1021 tgcctcgacc gctgttacgg aaatattctc gtccagcgac tgcaggacct ggggcgtcgc
     1081 cgtgttcaca ttgatcgtgg tgcggccggg cagggccgtg atgtggggcg ccaggatgtc
     1141 gaaggtcacc ttgtccatgc cctccagcgc ggccagctcg ctgatgctgg tgatgctctg
     1201 gttcgcgctg cgatacggcg ggaccatgcc ggtgtagata gggtcctcgg cgccatccgg
     1261 gaactgggca tcgagatccg cgtccagcca gtccgaagcg atgccggcga agcgcggatc
     1321 cagttccagg gcaacgagca ggcggcgaaa ctgttcgacg gcttcctcgt cgaccgcgcc
     1381 gccttcgtct atgaggttgt tgatgttgaa ccggccctgg aggtcttcga cgtgtccgaa
     1441 aacctcgccg ccgtcgatcg gcaggcccgg gagctccgtc gcccagattt ccgccaaatg
     1501 atcggtctgg ctgtcctcca tatcctggtg aaggatgttg atcacccagc tttccgcgcc
     1561 cagcgccacc tgtaccgcct ggtcatggtt caggagcacc atcgtgcgcc tgacatcgag
     1621 cgtgttgtcc cacgcaaggt tgtacgcgac aatcgccgcg agagcggtga tgagcatggc
     1681 cgtgatgagg gcaactccgc ggttcctgcg gttgcgcact accatccgtg cgttcacggg
     1741 gaaacctcga gaagccgccg tatttccgtt ccatccgcca ggaccaggat gatttcgacg
     1801 gctcgagggc gctgcctcaa gccgaggagg ccgggccgat tctccggcgg ccattcttcg
     1861 ctccagtctc cgttgtcctg catgaagcga aacaggatgc tgtccacttc gtccagcagc
     1921 acgcgcccgg cgggctcgtt gctcaaggtc cggtccagca cggtccagtg ataacggatg
     1981 agttcacctt cctcgagacg ataggcgacg cgttgctgcg tcccgcgggg cagcgtggcg
     2041 ggattactcc agccgccatg tgtcagttcg acggcaaagt ccgactccac gtcggtatgc
     2101 aaggcgggtc cgaaattctc gccgagatcc tcgcgcaccg gccgcggcgc gagctgcatg
     2161 aaatcctcgc tgagataccg tacggtccgc tgcagcgact ggaggcgctg catccgatcg
     2221 gtcaggatct ctgcattcgc cagtgtctgc ccgagcgttc cgtaggcgat agcggcaagc
     2281 atgccgaaga tcgcaagggc aaccagcacc tcgaccaggg tgaagcctga ctgctgcggc
     2341 ctgctcaacg ttcttccccg gcgggcagat tcgtcgtcca ggcgatgttg ctctggcccg
     2401 ggattcccgg ttcgccgatg aagccggtca cgctgcggat gatcgcttcg gaatcgacga
     2461 aggagactgc aacgtccacg cggaacaggt tttcgacgcc cgtttcggag accgtgctcc
     2521 gccaggtcca ttccaggccg gcgaactcta cctctcccga atcctcgctg acgtcgggga
     2581 cgacattgga cagccgcatt tccgcgatct tgttctgggc tatccagctc gcataggtgc
     2641 gctctctcat gctgctggcg gcatccacca tctggctcat cgcagcggtc accgccgtta
     2701 gcgacagcgc cgcgatgccc agcgccacca tcacctcgag caaggtgaag cccgcggagc
     2761 ggcgccggtc agagcgtcgc cgattcatcc gcgttgatct cgaactcgcc gttcagggtc
     2821 atttccatcc gtacttcgga acggtcggcc cagcgaacga ggcgcaggtg aaaaggcgtc
     2881 acttcgccac tggaaagtat cagcacatgc ggcaggtagc tattacgttc ccggtctccg
     2941 gaatcctccc cgacatcggt atccgcggct tcctcggcaa gcgagaccgg cctgtcctcg
     3001 aggaacaact cgaactccat gccttcctgg agcggacgcg gtcgcagcag ctcgtcgccg
     3061 acaacttcga gccactggtc gagcgccgga tcatgttcta cgaagcggta gccgtgctgc
     3121 atgaactcaa ggccaaagtc gcggccctgc atgattgctt cgtccgttgc gagttcgatc
     3181 agcgacgaga tgcgtcgcgc ctgttgctgc aacgccctgt cgtcgcccag gatgccgaac
     3241 gacatcaaca gcatcgcgct gatgatggcc atgatgagca ccaccacgag gacttcgatc
     3301 agcgtaaagc cgcccctctt tcgaacggca ggtctcatga cggattattc gagttcccag
     3361 ttgccgatgt cagcatccag gccttcgcca ccggggcggc cgtcccggcc aagcgtgtag
     3421 acgtcaattt ctccgtgatt acccggattc aggtaaaggt aaggatttcc ccacggatct
     3481 ttcggtgcac ggtccaggta cccgcccgct ttccagttgc ggatgttggg gtcggccggc
     3541 ttcgtcacca gcgcctcgag gccctgttcg ctcgtgggat agctgaagtt gtccatgcga
     3601 tagaaattga gggcactttc gatgcctctc aggtctgact ggacccgcgt gatctgtgca
     3661 tcgtcgatgc gcccgatcac atttggtgcg acgattgccg ccagtatgcc gaggatgatc
     3721 accacaacca tgatctcgat cagcgtgaac cctcgctgcc tgcgattgcg aaccccggtt
     3781 ttgtgcactg taaatactcc gaatacttcg tctgggacgt tcggcctgcg ttgacggccc
     3841 ctagtgcgct gtctcgcaaa taacgttaca gtttcgctgc ctctttttgc gtctggcggc
     3901 gttgcgaca
//
LOCUS       DASUOM010000383         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_986572,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000383 DASUOM010000000
VERSION     DASUOM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986572"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFY27_07965"
     CDS             complement(<1..824)
                     /locus_tag="VFY27_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX5765489.1"
                     /translation="MSVRSSSDIDVLLVVASIRAHWRAIAAGLVVGMLAAATYLLIAD
                     RIYRVDVVVTSTADDEQSPLSGLAGALGGLSALGGELLGNANLENESIALLHSKTIAM
                     ALISRNDLIPILFEEKWHSENDEWQVSADDIPTMADAVERLHEDVIRVRRDRELGLIT
                     ISAYWRNKELASDWAMQMISLVNEKMRRQAIDEATQSLAYLAEELEKSQNVEARQMIS
                     QLMQSKLNNSAMASAKREYAFRIIDPAILPDDDDYVRPNVILAVVGSTLLGMILGFT"
     gene            complement(846..>2853)
                     /locus_tag="VFY27_07970"
     CDS             complement(846..>2853)
                     /locus_tag="VFY27_07970"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX5765490.1"
                     /translation="NLPEIGPVPVAGITFAEFRADVNERVKQMLIGTQVSVSMGALRT
                     IRVFVLGDVKRPGSYVVGGLATISSALYESGGISEIGSMRSIQLKRQGNVVATLDLYD
                     LLLRGDTSGDARLQPGDVIYIPPIGDTIGVGGAVLRPAIYESRNVTTAGEAVQFAGGL
                     TPDAFPKGARLERFTADGQRIVMSVDLSSETGLEQPMRAGDILMIPEVLPDLEGAVTL
                     VGHVQRPGPYEWRPGMRLTDLISEPEELKQGVDDNYVLVRRERVRGRPIEVLSADLGA
                     ALVNPAGEENIPLEARDTVYVFSLSYGRQRVISPLLDELQLQATHDKPLQQVQVGGFV
                     RAPGAYPLEPGMRVSDLIRAGGRLTEQAYTLEAELSRYSVVNGELRVTDISVIDLDSI
                     LAGDETADAVLAAHDHLSINRVPEWESTWSVTLDGEVTFPGAYRIRRGETLSEVLERA
                     GGLTDEAFPEGAVFLRETLREREQEQIDALARRMESDLISLSLETLDTTGAQALSTGQ
                     QLLQQLRETDAVGRLVIDLERAADPDERRNLVASVELRDGDRLLVPKQSQEVTVIGET
                     QLNTSHLYREGLSRDDYIELSGGLTRRADKRLIYVVRASGAVVSSNRSLWFGRGADEV
                     IRPGDTIVVPLETDRIRPLTFWGSVTQILYQGAIAVAAVKTFDN"
BASE COUNT          525 a          929 c          844 g          555 t
ORIGIN      
        1 gtgaaaccca gaatcattcc gagcagagtc gagccaacga cagccagaat gacattcggt
       61 cgcacatagt cgtcatcgtc cggcaggatc gccggatcga tgattcgaaa ggcgtactcc
      121 cgcttcgcac ttgccatcgc tgaattattg agcttcgatt gcatgagctg cgagatcatt
      181 tgccgtgcct ccacgttctg gctcttctcg agctcttcgg ccaggtatgc gaggctttgc
      241 gtggcctcgt cgatcgcctg gcgtcgcatt ttctcgttta ccagtgaaat catctgcatc
      301 gcccagtcgg aagccagctc cttgttgcgc cagtaggcgc tgatggtgat cagcccgagt
      361 tcgcgatccc gccgaacgcg gatgacgtcc tcgtgcagcc gttcgacggc gtccgccatg
      421 gttggaatat cgtccgcaga tacttgccac tcgtcgtttt cgctgtgcca cttctcctca
      481 aacaatatcg gtatgaggtc gtttcgggat atcaatgcca tcgcgatggt tttcgagtgc
      541 agcagcgcaa tgctttcgtt ctcaagattg gcgttaccca acagctcacc gcccagtgcg
      601 gataatccac ccaatgcgcc ggcaaggcca gacagaggag actgctcatc atcggcagtc
      661 gaagtcacga cgacatcgac cctgtaaatc ctgtccgcga tgaggagata tgtagctgct
      721 gccagcattc cgacgacgag cccggcagca atcgctcgcc agtgagcacg gatgcttgcg
      781 acgacgagca gaacgtctat gtccgaagat gatctcacgc tcatggtcct gcgcccgtag
      841 tcgccctagt tgtcaaacgt cttgaccgct gctaccgcga tcgcaccctg gtacagaatc
      901 tgcgtgacac tgccccagaa tgtgagcggc cggattcgat ccgtttccag cggtaccacg
      961 atcgtgtcgc ccggccggat gacctcgtcg gcgcccctgc cgaaccacag ggatcggttg
     1021 gaactcacga ctgcgccgct cgcccgcacg acgtagatca gccgcttgtc ggcacggcgc
     1081 gttaatccgc cgctgagttc aatgtagtcg tcgcggctca ggccttcacg gtagagatgc
     1141 gatgtattca gctgcgtttc gccgattacc gtgacctcct gcgattgctt cggcaccagg
     1201 agccggtccc cgtcccgcag ttcgacagag gccacgaggt tccgtcgttc gtccggatcg
     1261 gcagctctct ccaggtcaat gacgaggcga ccaacggcat cggtctcgcg caattgctgc
     1321 agcagctgct gtcccgtcga cagcgcctgg gcaccggtcg tgtccagcgt ttcgagcgac
     1381 agggagatga ggtccgactc cattcgtcgc gccagcgcgt cgatctgctc ctgctcccgt
     1441 tccctgagag tttcacgcag gaataccgcg ccctcgggga aggcttcgtc ggtgaggccg
     1501 ccggctcgtt ccagcacctc gctcagcgtc tccccacggc ggatccggta ggcgccggga
     1561 aacgtcactt ccccgtcgag agtcaccgac catgtcgatt cccattccgg cacgcgattg
     1621 atgctcaaat gatcgtgcgc ggcgagcacg gcatccgcgg tttcatcgcc ggcaagaatg
     1681 gagtcgagat cgatgacgga aatatcggta acgcggagtt ccccatttac gaccgagtaa
     1741 cggctcagtt ccgcctcgag agtataggcc tgctcggtca gccggccgcc ggcccggatc
     1801 aggtcgctga cccgcatgcc gggctccagc ggataggcgc cgggagcccg cacgaagccc
     1861 ccgacctgca cctgctgcaa cggcttgtca tgcgtggcct gcagttgcag ttcgtcgagc
     1921 aagggggaaa tcacgcgttg ccgtccgtag gacaggctga acacgtacac cgtatcgcgc
     1981 gcttcgagcg gaatgttctc ttcgccggcc ggattcacca acgcggcccc gagatccgcg
     2041 gacagcacct cgatcgggcg cccgcggacc cgctccctgc ggacgagcac gtagttgtcg
     2101 tcgaccccct gcttgagttc ctccggctct gatatgaggt ccgtcagccg catccccggg
     2161 cgccactcgt acgggccggg gcgctggaca tgaccgacca gcgtcaccgc cccctcgagg
     2221 tcaggaagga cctcggggat catgaggatg tccccggcac gcatgggctg ctcgagtccg
     2281 gtctcgctcg aaaggtccac ggacatcacg atccgttgtc catcggccgt aaaccgttcg
     2341 agccttgcac cttttggaaa ggcatcgggc gtcagcccgc cggcgaactg cacggcctcg
     2401 ccagcggtgg tgacattgcg agactcgtag atggccggtc gcagtacggc gccaccgaca
     2461 ccgatcgtat cgccgatcgg aggaatatag atgacatcgc caggctgcag ccgggcgtca
     2521 cccgatgtat cgccgcgaag cagcaggtcg tagagatcca gagtggccac cacattgccc
     2581 tggcgcttca gctggatgct gcgcatcgag ccgatctcgc tgattccgcc gctctcgtac
     2641 agcgcgctgc tgatggtcgc caggccgccg accacgtaag agcccggtcg cttgacgtcg
     2701 ccgagcacga acacccggat ggtgcgcaat gcacccatgg agacgctgac ctgggtaccg
     2761 ataagcattt gcttgacgcg ctcgttgacg tcggcgcgga actcggcgaa cgtaataccc
     2821 gcgaccggaa ccggcccgat ctccggaagg ttg
//
LOCUS       DASUOM010000384         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_211163,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000384 DASUOM010000000
VERSION     DASUOM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211163"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VFY27_07975"
     CDS             complement(<1..1820)
                     /locus_tag="VFY27_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765491.1"
                     /translation="MHCKHSIKTALWSLVVAACLTQPAALAQHAGEQTGGHIGEPLVV
                     KDARYGEILFYFYQEEYFPAIVRLQAAKERSQLENHDAEAELLLGGLYLSYGHHLRAA
                     EIFERLLAGNTTPAIRDRTWFFLAKIWYQRGYLDESLKALANIEGALDDAMDAEKRML
                     RAQVLIDSGAYDEAIADLAGWEGESGWESYAKFNLGVAMVRSGRVDEAASLLDAVGNI
                     RPDSEELSALRDKANLALGYAYLQGDQPVAAKPLLQRVRLEGPFSNKALLGVGWADAE
                     TADYRQALVPWMELRSRDLLDSAVQESMLAIPYAMARLDSIGQAADYYVSAIDAFHEE
                     SRRIDQVIARIEQGALVDSFVAEDRNAATGWYWKLDALPEGTESRYLYHLLATHKFQE
                     GLKNFRDLRYLSKNLAGWQESVGVFRSMLDTRELAYTERLPGTTSRLAAADIESLVDQ
                     KLEFDARLNIIEENGDSLALATEREFDMWGEITALERSPAFASNTPEAAEARKKVALL
                     KGVLQWDLDRQFNDRLWHIRRRLRQAGEGLVETQRARRGVDAAMREEPAQFAGFGDRI
                     EAIGPRIDSLQRRVDKAVRQHRIFLQDVAVEELQAQKQRL"
     gene            complement(1824..2408)
                     /locus_tag="VFY27_07980"
     CDS             complement(1824..2408)
                     /locus_tag="VFY27_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5765492.1"
                     /translation="MSIKRCFGVAVVCAWFALGHATSFAQQAGSAAASAGATEASADA
                     GEFRSLDEDVQSLKEEVLALNRDLFLLEEELLFPANSQVAFFISMDVGEYFELDSVNL
                     KIDGKEVANYLYTERELGALLRGGVHRVHMGNLKVGDHELVAVFTGHGPNAREFRRGA
                     TMNFNKGIGAKYVELEITDRVSKQQPEFAIKEWE"
     gene            complement(2462..2974)
                     /locus_tag="VFY27_07985"
     CDS             complement(2462..2974)
                     /locus_tag="VFY27_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5765493.1"
                     /translation="MKRMRIAVLGALLVAVPGIFAASSFASSGLAGQEAPDFALKSAS
                     GSNLRLSEYRGDVVMINFWATWCGPCRQEMPLLNDLYARYNRVGFSLLGVNIDDDSSR
                     ALEMAEELGVSFPVLFDERKEVSKLYQVEAMPVTVLVDREGKVRHVHLGYKPGYEEKY
                     LTQIRALLRE"
     gene            complement(3134..4027)
                     /locus_tag="VFY27_07990"
     CDS             complement(3134..4027)
                     /locus_tag="VFY27_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008222139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5765494.1"
                     /translation="MGTEISVQLWHDDSEAADRAVNAVFVEMERINELMSTYIEASRI
                     SLINREAADRPVPAGDELYDLIVRSLDMSILTRGAFDITYDSVGQHYDFRQKQRPDPA
                     TLEQELIHIDYRLIETHPSTRSIRFLQQGVRINLGGIAKGYAVERGVGILRDLGIRHA
                     IVTAGGDSRLLGDRRGQPWMVGVRDPRNDGKVAIRMPLENEAISTSGDYERYFEQDGK
                     RYHHIIQPATGEPANGIHSATIIGPDAVITDALSTSVFVMGVDQGLRLISTLPDYEGI
                     VIDAEGKLFYSDGLQPPEQNL"
     gene            4162..4752
                     /locus_tag="VFY27_07995"
     CDS             4162..4752
                     /locus_tag="VFY27_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5765495.1"
                     /translation="MTSFLVFLAIIVAVIAFGIAIYNNLVNLRNRVKNAFAQIDVQLT
                     RRYDLIPNLVEAVKGYMKHERETLEAVIKARNAAVSSLDAAKADPSNADAIKKLGAAE
                     GLLGGALGRLFALSEAYPDLKANQNMMQFQEELASTENKVAFARQAFNDSVLSYNNAV
                     ENVPNNIIAGMFRFESASFLDIDSEQKREVPEIAFT"
BASE COUNT          922 a         1481 c         1381 g          981 t
ORIGIN      
        1 tccaggcgct gcttctgcgc ctgcaattcc tcgaccgcaa catcctgcag gaagatcctg
       61 tgctggcgga cggccttatc gacgcggcgc tgcaacgagt ctattctcgg gccgatcgcc
      121 tcaattcgat cgccgaaccc cgcgaactgc gcaggctcct cccgcatggc cgcatcgacg
      181 ccgcgccgcg cccgctgcgt ctcaaccaga ccttccccgg cttgccgcag gcgcctgcga
      241 atgtgccaca gccggtcgtt gaattgcctg tcgaggtccc attgcagcac gcccttcagg
      301 agtgcgacct tcttccgcgc ttccgcggct tccggcgtat tcgaggcaaa cgcaggactc
      361 ctctcgaggg cagtaatttc accccacata tcgaactcgc gttcggtagc aagtgccaac
      421 gagtcaccgt tttcttcgat gatattgagc ctcgcgtcga attcgagctt ctggtccaca
      481 aggctttcga tgtccgccgc cgcaagcctc gaggtcgttc cagggaggcg ctcggtgtag
      541 gcaagttcgc gggtgtccag catgctgcgg aaaacgccga cgctttcctg ccaccctgcc
      601 aggttcttgg agagatatcg tagatcgcga aagttcttta gcccttcctg gaatttgtgc
      661 gtcgcaagca ggtggtacag gtaacgagac tcggtccctt ccggaagcgc atcgagcttc
      721 cagtaccatc ccgtcgcagc attgcggtcc tcagccacaa aggaatcgac cagcgctccc
      781 tgctcgatgc gcgcgataac ctggtcgata cgacggctct cttcatggaa ggcgtcgatc
      841 gccgagacgt agtagtcggc agcctggccg atggaatcca gacgcgccat tgcgtaagga
      901 atcgccagca tggattcctg cacggcggag tcaaggaggt cccggctgcg caattccatc
      961 catgggacga gcgcctggcg gtagtctgca gtttcggcat ctgcccagcc gacacccagc
     1021 aatgccttgt tcgagaatgg cccttcgagt cgcactcgct gcaacagcgg ctttgccgcc
     1081 acgggctggt ctccctggag gtaggcgtac ccgagcgcaa ggttcgcctt gtctcgcagc
     1141 gcgctcagct cctcgctgtc cggacgaata ttgcctacgg cgtcaagcag tgatgcggct
     1201 tcgtccacgc gtccgctccg gaccatcgcg acgccgagat tgaactttgc ataactttcc
     1261 cacccggact cgccttccca accagcaaga tccgcgatgg cctcgtcgta cgccccgctg
     1321 tcaatgagga cctgcgcgcg cagcatcctc ttttcagcat ccatcgcatc gtccagcgcg
     1381 ccttcgatgt ttgcgagggc cttcagggac tcatcaaggt acccgcgctg gtaccagatc
     1441 ttggccagga aaaaccaggt cctgtcgcgg atcgcgggag ttgtattgcc ggcgagcagc
     1501 cgttcgaaga tctcggctgc gcgaaggtga tgcccgtatg aaagataaag gccgccgagc
     1561 aggagttccg cctcggcatc gtgattctcg agctgcgagc gttccttcgc cgcctgcagg
     1621 cgcactatcg ccgggaaata ctcctcctgg tagaagtaga acaggatctc gccgtacctg
     1681 gcgtccttga cgacgagcgg ctcaccgata tggccgcctg tttgctcgcc cgcatgctgg
     1741 gcgagagccg ccggttgcgt gaggcacgca gcaacaacga ggctccacag tgcagtcttg
     1801 atggagtgct tgcagtgcaa ggatcactcc cattctttta tggcaaattc aggctgctgt
     1861 ttcgaaaccc tgtctgtgat ctccagttca acgtacttgg cgccaatgcc cttgttgaag
     1921 ttcatcgtgg cgccgcggcg gaattcgcga gcgttcggtc catgcccggt aaacacggca
     1981 accagttcgt gatctccaac tttgaggttt cccatgtgca cgcgatgtac gccgccgcgc
     2041 agcaaggcgc ccaattcgcg ctcggtgtag aggtagttgg caacttcctt gccatcgatc
     2101 ttcaggttca ccgagtcgag ttcgaagtat tcaccgacgt ccattgagat aaagaacgcc
     2161 acctgcgaat ttgccggaaa aagaagctct tcctccagca ggaacaagtc acgattcaag
     2221 gcaaggacct cttccttgag cgactgcacg tcttcgtcga gactccggaa ttccccggcg
     2281 tcagcggatg cttccgtcgc acccgcgctg gccgctgccg agcctgcctg ctgcgcgaac
     2341 gaggttgcat ggccgagtgc gaaccacgca cagactactg caactccgaa gcatcgcttt
     2401 atcgacacga taattctcct gcccgttttc cacgcccgac gaaggccaag ccgttaacgg
     2461 attactcccg cagcagggcg cgaatctgcg tgaggtattt ttcttcgtac ccgggcttgt
     2521 agccgagatg cacgtgccgc accttgccct cccggtcgac cagcaccgtc accggcatcg
     2581 cttccacctg gtaaagcttg ctcacctcct tgcgttcgtc gaacagcacc gggaaggaga
     2641 cacccagttc ctctgccatc tccagggcgc ggctggagtc atcgtcgatg ttcacgccca
     2701 gcaggctgaa tccaacccgg ttgtagcgcg cgtaaagatc gttgagcagc ggcatctcct
     2761 ggcggcacgg accgcaccac gtggcccaga aattgatcat cacgacgtcg ccgcgatatt
     2821 cgctcaatcg aaggttgctg ccgctcgcgc ttttcagcgc gaaatcaggc gcctcctgcc
     2881 cggcaaggcc ggacgacgcg aagctgcttg ctgcaaagat tcccggcaca gccacgagca
     2941 gagcaccgag cactgcgatt ctcatccgtt tcatgaaccc gtccattttc agccgcacgc
     3001 catggtgaca gctgaggtcc ggtagaggcc gtgaccaccg tcacaaatga acgcgcgcac
     3061 cggaccataa cgcgtctgca aacagagacg tacgcggttt tcaacgccgg aagacgcagg
     3121 tccgcggctg ctgctacagg ttctgttcgg ggggttgcag gccgtcggag tagaagagtt
     3181 tgccttcggc gtcgatcacg atgccttcgt agtcgggcag cgtggagatc aggcgaagac
     3241 cctggtcgac gcccatgaca aacacggaag tcgacagcgc gtccgtgatg acggcgtcgg
     3301 gaccgatgat ggtagcgctg tggatgccgt tggcgggttc gccggtggcg ggctggatga
     3361 tgtggtgata gcgcttgccg tcctgctcga agtagcgctc gtagtctccg gatgtggaga
     3421 tggcttcgtt ctcgagcggc atcctgatcg cgaccttgcc gtcgttgcgc gggtcacgga
     3481 caccaaccat ccagggctgt cccctccggt cgcccagcaa gcgcgagtcg ccgcctgcgg
     3541 tgacgatcgc gtgcctgatc cccaggtcgc ggaggatgcc gacgccgcgt tcgacagcgt
     3601 atcccttggc tatgccgccg aggttaatac gaacgccttg ttgcaggaaa cgaatcgacc
     3661 tggtggaggg gtgggtttcg atcaaccgat agtcgatatg tataagctcc tgctccagcg
     3721 tcgcgggatc agggcgttgc ttctgccgaa aatcgtaatg ctggccgacg ctgtcgtagg
     3781 tgatatcaaa cgctccgcgc gtgaggatgg acatgtcgag cgaacgcacg atgagatcgt
     3841 atagttcgtc gcccgccggt accgggcgat cggctgcctc gcggttgata agcgaaatcc
     3901 ggctggcttc gatataggta ctcatcagct cattgatgcg ctccatctcg acgaacaccg
     3961 cgttcacggc tctgtctgcc gcttccgagt cgtcatgcca caactggacg ctgatttccg
     4021 tgcccatgag cggccggctg tcgccatgcc attcggcgtc ggctgcgccg ggccggaggg
     4081 caagcaggct tatggttgct agtgttaggc cggctcgaag gttattcttg cggcgagcag
     4141 aaatcacccg ggaggtggat tgtgacgtca tttctggttt tcctcgcgat aatcgttgcg
     4201 gtcatcgcct ttggaatcgc gatctataat aatctcgtca acctgcgaaa tcgcgtcaag
     4261 aatgcctttg cccagattga cgtccagctc acccgacgct acgacctcat accgaacctc
     4321 gtcgaagccg taaaaggcta catgaaacac gagcgtgaga cactcgaggc cgtcatcaag
     4381 gcgaggaacg cggcggtctc cagtctcgat gccgccaagg cggatccctc gaacgcggac
     4441 gctatcaaaa agctgggagc ggctgaagga ctgctgggcg gtgcgttagg ccggctgttc
     4501 gccctttcgg aagcctatcc ggacctcaag gccaaccaga acatgatgca gttccaggag
     4561 gaactcgcca gcaccgaaaa caaggtcgcc ttcgcacggc aggcgttcaa cgattcggtg
     4621 ctctcctata acaatgcagt ggaaaacgtc ccgaacaaca taatcgccgg catgtttcgc
     4681 ttcgagtctg ccagcttcct cgatatcgat tcggagcaga agcgcgaggt tccggagatc
     4741 gcgtttacct aggaatatcc gtcgt
//
LOCUS       DASUOM010000385         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1576839,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000385 DASUOM010000000
VERSION     DASUOM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576839"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFY27_08000"
     CDS             <1..486
                     /locus_tag="VFY27_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5765496.1"
                     /translation="VVEAAKRSGSLITARLAAEQGREVFAIPGSIHNAMARGCHQLIR
                     QGAKLVESADDIASEFGPLVAHLMQNANRDSRRKVVDAAATRHDADYDRLLAALGHDP
                     ATVDEIARQSGLTIGEVSSMLLILELEGEIEALNSGQYARLGSSSRHERERARRADVS
                     F"
     gene            446..922
                     /locus_tag="VFY27_08005"
     CDS             446..922
                     /locus_tag="VFY27_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX5765497.1"
                     /translation="MKENVLDVLMYLFETYIDAEEEPEPDQNELRDELSRAGFADSEI
                     DRALDWLDGLAENQGGLNYSPQVGHGTRIYNEMEHERLDVGCRGFITYLEQIGILSPP
                     QREILIDRLLALETPDIDVEQIKWVVLMVLFSQPGQELAYARMEDLVFDESGGALH"
     gene            1100..>3270
                     /locus_tag="VFY27_08010"
     CDS             1100..>3270
                     /locus_tag="VFY27_08010"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019570205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX5765498.1"
                     /translation="MPKNLVIVESPAKAKTIGRYLGEDFDVLASYGHVRDLVPKEGAV
                     DPANGFAMKYRLIEKNEKHVNAIVRALKKADALYLATDPDREGEAISWHLTELLKEKN
                     ALNGKDVYRVVFHEITRGAIQEAIAHPRKISDDLVSAQQARRALDYLVGFNLSPLLWK
                     KVQRGLSAGRVQSPALRMIVEREIEIEAFKAREYWTIEADVSKEAQPFVSRLVRYRGE
                     KVEQFSFGNEEQAREVERTILDAADGKLTTVSVDKKKRRRNPAAPFTTSTLQQEASRK
                     LGFNTQRTMRVAQRLYEGIELQGEGNVGLISYMRTDSVTLAATAVDEIRGVIRERYGD
                     TNVPETQPEYKNKSKNAQEAHEAIRPTSAKRTPESLQNKLDPDQLKLYTLIWQRTMAS
                     QMVPAVFDTVALEFAAGPDTENGHRFRANGSVLVEPGFIAVYQEGLDDAGAEEGDRLL
                     PEVNEGDVISLDKLRPEQHFTEPPPRFTEASLVKTLEEYGIGRPSTYASIIATLKNRE
                     YVEMDGKRFIPTDIGRIVNGFLTEHFTQYVDYDFTAKLEDDLDAISLGEKDLVPLLDE
                     FWKPFSELVMDKEQSVSREQVAQARDLGTDPKSGKPMTVRMGRYGPFVQIGTKDDEEK
                     PKFAGLRPGQKMNDITQEEALELFKLPRMLGETPDGLPVMASVGRFGPYVRYGDKFVS
                     MRGEDDPYTIQLPRALELIEEKKKADASRLIQDFEDEGIQVL"
BASE COUNT          760 a          938 c         1007 g          565 t
ORIGIN      
        1 gtggtagaag cggcgaaacg cagcggttca ctcatcacgg cgcggctcgc tgccgagcag
       61 ggccgtgaag tctttgcgat tccgggctcg atacataatg caatggcgcg cggctgccac
      121 cagctcattc gccagggcgc gaaacttgtc gaaagtgcgg acgacattgc gagtgaattc
      181 gggcccctgg ttgcgcacct tatgcaaaat gccaaccggg actcgcgacg gaaagtggtt
      241 gatgcagcag ctacgcgaca cgatgcggat tacgacaggc tgctcgcggc gctcggtcac
      301 gatcctgcaa ccgtcgacga aatcgcccgg caatccggtt tgacgatcgg cgaggtttcc
      361 tccatgcttc ttatcctgga gctcgagggc gaaatcgaag ctctgaacag cggccagtac
      421 gccagattag ggagtagctc caggcatgaa agagaacgtg ctcgacgtgc tgatgtatct
      481 ttttgagaca tacatcgacg ccgaagaaga gcctgaaccg gaccagaacg aacttcggga
      541 cgagctctcc agggccggat tcgcagacag tgaaatcgat cgcgctctcg actggctcga
      601 cgggctcgcc gaaaaccagg gcggcctgaa ctatagcccc caggtcgggc acggcacccg
      661 gatctacaac gaaatggagc acgaacgtct cgacgtgggt tgccgtggtt tcatcaccta
      721 cctcgagcag attggcatcc tgtcgccgcc gcaacgcgaa atcctgatcg acaggctgct
      781 cgccctggaa acgccggata tcgatgtcga acagatcaag tgggtggtgc tgatggtcct
      841 gttcagccag cccgggcagg aactcgccta cgcgcgcatg gaagatctcg tattcgacga
      901 aagcggcggc gcgctgcact gacggaaagc gtcccgtatc gcacgccagt acccgatttc
      961 ttcgctttta ctgcgcttcc gggtcggcca tccgaatggc tcgggcttga cagcctgcag
     1021 gcaagcatgt aattcaaccg ccccacagtg gcgccgttcc ctgacgtaca cgcgccaatt
     1081 caccggatcc ctggaagaaa tgccgaagaa tcttgtcatt gtcgaatcgc cggcaaaggc
     1141 caaaacgata ggcaggtacc ttggcgagga cttcgacgtc ctcgcgtcgt acggccatgt
     1201 tcgcgatctc gtgcccaagg aaggcgcggt cgatcccgcg aacggcttcg cgatgaagta
     1261 ccggctcatc gaaaaaaatg aaaagcacgt caatgctatc gtgcgggcgc tcaagaaggc
     1321 cgacgcactg tacctggcca ctgacccgga tcgcgaaggc gaagcaattt cctggcacct
     1381 gacggaactc ctgaaggaga aaaacgcgct caatggcaag gacgtctacc gtgtggtgtt
     1441 tcatgagatc accaggggcg ccatccagga agctatcgcg catccccgaa aaatatccga
     1501 cgacctggta agtgcccagc aggccaggcg ggcactggat tatctcgtcg gcttcaacct
     1561 gtcgccgctg ctctggaaga aagtgcagcg cggattgtcc gcgggacggg tgcagagtcc
     1621 ggcgctccgg atgatcgtcg agcgcgaaat cgaaatcgaa gcgttcaagg cccgggaata
     1681 ctggacgatc gaagccgacg tcagcaagga ggcccagcct tttgtttcgc ggctcgtgcg
     1741 ctaccgtggc gaaaaggtcg agcagttcag tttcggcaat gaagaacagg ctcgcgaggt
     1801 agagcgaacg atactggacg ccgcggacgg caagcttacg acggtcagcg tcgacaagaa
     1861 gaagcgtcga cgcaatcccg ccgcgccgtt cacaacatcc accttgcagc aggaggcctc
     1921 gcgcaaactc ggctttaaca cgcagcgcac gatgcgcgtg gcacagagac tgtacgaggg
     1981 catagagctt cagggtgaag ggaacgtcgg tctcatcagc tacatgagaa cggactccgt
     2041 cacgctggct gcgacggccg ttgatgagat tcggggcgtg atccgcgagc gttacggaga
     2101 cacgaacgtt ccggaaacgc agcccgagta caagaacaag tccaagaatg cccaggaagc
     2161 gcacgaggcg attcgcccga cttcggccaa acgcacgccg gaatcgctgc agaacaagct
     2221 cgacccggac cagttgaagc tctacacgct gatctggcag cgaacgatgg catcgcagat
     2281 ggtaccggcg gtgttcgaca cggttgcact cgaatttgct gccggcccgg ataccgaaaa
     2341 cggtcaccgc ttccgtgcca atggttcggt gctcgtcgag ccgggcttca tcgcggtgta
     2401 ccaggaagga ctcgatgacg ccggcgccga agagggtgat cgactgttgc cggaggtaaa
     2461 cgaaggcgac gtcatctcgc tcgacaagtt gcgcccggaa cagcatttca ccgagccgcc
     2521 gccgaggttc acggaggcga gcctggtcaa gactctcgaa gagtacggaa tcggccgtcc
     2581 gtccacttac gcgagcatca ttgcgacact gaagaatcgt gaatatgtcg aaatggacgg
     2641 caagcgtttc atcccgaccg acatcggcag gatcgtgaac ggattcctga cggagcactt
     2701 cacgcagtac gtcgattacg attttacggc gaagctcgaa gacgacctcg atgccatctc
     2761 cctcggcgag aaggatcttg ttccgctgct cgacgagttc tggaagcctt tttccgagct
     2821 cgtcatggac aaggagcaaa gcgtttcgcg cgaacaggtc gcgcaagcga gggacttagg
     2881 gacagacccg aaaagcggca agccgatgac ggtgcggatg ggccggtacg gaccgttcgt
     2941 ccagatcggc acgaaggacg acgaggagaa gccgaagttc gccggtttgc ggcccggcca
     3001 gaagatgaac gacatcacgc aggaggaagc gctggaactg ttcaagttgc cacgcatgct
     3061 cggggagacg ccggacgggt tgccggtgat ggcgagcgtc ggacgcttcg gtccctacgt
     3121 acgctacggc gacaagttcg tctcgatgcg cggagaagac gacccataca cgattcagct
     3181 accgcgggcg ctcgaactca tcgaggagaa aaaaaaggcg gatgcaagcc ggctgatcca
     3241 ggattttgaa gacgaaggaa tccaggtcct
//
LOCUS       DASUOM010000386         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1128774,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000386 DASUOM010000000
VERSION     DASUOM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128774"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFY27_08015"
     CDS             <1..196
                     /locus_tag="VFY27_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5765499.1"
                     /translation="KWNNVRAALDIARDARDMLGGAGISAEHVPIRHMLNLESVITYE
                     GTETVHQLVVGKELTGVSAF"
     gene            237..2303
                     /locus_tag="VFY27_08020"
     CDS             237..2303
                     /locus_tag="VFY27_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5765500.1"
                     /translation="MEILIQMESTERGLPGIQRDSSSTVRAAIDAMRANRPLRAEEIC
                     RNYLLLNPGSAQHLRLLGHALMKQSRLEEAADQLRFALSLEPENPHLHEDIGSVLALQ
                     QRFPEAIACFEKAIALEPRLPLAHKKLGQALAALGRGHDADAAFEEYFERDPEKGIVA
                     IGAQHLKAGRKDEAIQCFRNALQENPDNVDAMRCLGNVYLRDKEHLSDAEALLRRATQ
                     VAPDFTAAWILLGGVLHERKRNVEAIDCYRKAVELEPDNAFAWGALGNAFAFAGYPNR
                     GAEAYAKSVELDPKAPTMQMGYAHVLKTLGDQAGSLSAYRAAIKAKPDFGEVYWSMAN
                     LKVFRFDEDEVSAMEQQLKRDDLTASSEIHFRFALGKAYEDRKDYDTAWKYYDSGNKR
                     QRMQVTHDTQEMEIRQNDIIEVFSREFLEEHGGNGCDAPDPIFIVGLPRSGSTLIEQI
                     LASHSQVEGTAELANLGKITSTVGRYRPDNLRYPKALKDMRAKDWRAYGQQYIEETRR
                     HRLTDKPFFTDKLPNNFPHVGFLHLVLPRAKIINARRHPLDSCLGGYKQLFGKGQNFT
                     YDMFELADYYRQYHNTMQHWHRVLPGKVLDVHYEETVMDLEGQVRRILEHCGLPFEEA
                     CVRYYETARAVQTASSEQVRQPIYREGLGKWRRYEQYLDLWKEELADILDELPDTVRN
                     AGLQDS"
     gene            complement(2341..2817)
                     /locus_tag="VFY27_08025"
     CDS             complement(2341..2817)
                     /locus_tag="VFY27_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765501.1"
                     /translation="MLNKLHINNVVCNPEGMYIGGLRTGGMLHFNGKAIHMSAELPPG
                     SHNARPFRDGVLFNDSEANALRYAGRGEGGEDRAFRVPAYPQDLLTHVSEDSARLARQ
                     GFARGLCVLSDAVVAGGSSPSTITVYDLPSNERLLSVNLTMDIRNAIHGLEVWPYA"
     gene            2800..3108
                     /locus_tag="VFY27_08030"
     CDS             2800..3108
                     /locus_tag="VFY27_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765502.1"
                     /translation="MQLVQHERAIGAAGIVAQAAEPELVGRDVQAPLELSTVEVESQD
                     AVEAGRGQVKGAFINRDLVTVLEVAVAPAPDPLEVRRVGEEFIAGRDVERLPVERDAT
                     "
     gene            complement(3261..>3489)
                     /locus_tag="VFY27_08035"
     CDS             complement(3261..>3489)
                     /locus_tag="VFY27_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765503.1"
                     /translation="GIIPSSTTANFQVGVEMENEWDISLMVRNVWDEKNVSWLSNLNY
                     GDSLVFYDLRFNESHSLQKPRTFSLTVRKSF"
BASE COUNT          746 a         1017 c         1098 g          628 t
ORIGIN      
        1 gaagtggaac aacgtccgcg cggcgctcga catcgcccgc gacgcgcgcg acatgctggg
       61 cggcgcggga atcagcgccg agcacgtgcc gatacggcac atgctgaacc tcgaaagtgt
      121 catcacctac gaaggtacgg aaacggtgca ccagctcgtc gtcggcaagg agctgaccgg
      181 agtcagcgcg ttctgaactg gacctggcac cgccggccgg tcaagcggaa cccggattgg
      241 agattttgat ccagatggag tcaacagagc gcggcctccc cgggatacag cgggattcaa
      301 gttcaaccgt ccgtgcagcc atcgacgcca tgcgggcgaa caggccgctg cgagctgaag
      361 aaatatgccg gaactacctg ttgttgaatc cggggtctgc gcagcatctg cgcctgctcg
      421 ggcacgcact gatgaagcag agcaggctcg aggaggccgc agaccaactc cgctttgcgc
      481 tgtcgctcga gccggagaat ccacatcttc atgaagacat cggcagcgtt cttgctttgc
      541 agcagcgctt cccggaagcg atagcctgtt tcgagaaggc aatcgcactg gagccccgac
      601 tgcccctcgc tcacaagaaa ctcggccagg cacttgcggc tctgggccgc ggacatgatg
      661 cggatgcagc tttcgaggaa tatttcgagc gcgacccgga gaagggcata gtggcaatcg
      721 gtgctcagca tctgaaagcc gggcgcaagg atgaggcgat tcagtgcttc cggaatgcgc
      781 tgcaagagaa tccggacaat gtcgacgcca tgcgctgcct cggaaacgtg tacctgcgcg
      841 acaaggagca cctgagcgac gccgaggcgc tgttgcgccg ggccactcag gttgcgccgg
      901 atttcacggc agcgtggatc cttcttggcg gtgtactgca tgaacggaag cggaacgtcg
      961 aggccatcga ctgctatcgg aaggcggtcg aactggagcc ggacaatgct ttcgcgtggg
     1021 gagcactcgg taatgccttt gcgtttgccg ggtacccgaa caggggagcg gaagcctacg
     1081 cgaagtcagt cgagctggat ccgaaagcgc ccaccatgca gatgggctac gcgcatgtgt
     1141 tgaagacact gggcgaccag gccggctcgc tgtccgccta ccgtgccgct atcaaggcga
     1201 agcctgattt cggcgaggtt tactggagca tggccaacct caaggtattc cgtttcgatg
     1261 aagacgaggt ctcggcaatg gagcagcagc ttaagcgcga cgacctgacg gcaagctcgg
     1321 agatccactt tcgtttcgcg ctcggcaagg cgtacgagga caggaaagac tatgacaccg
     1381 cgtggaaata ctacgacagc ggtaacaagc gccagcgcat gcaggtgacg cacgatacac
     1441 aggagatgga aatccggcag aacgacatca tcgaggtttt cagccgggag ttcctcgaag
     1501 agcacggcgg gaacggctgc gacgccccgg atcccatttt catcgtgggc ttgccgcgct
     1561 ccggctcgac gctgatcgaa cagattctcg cgagccacag ccaggtcgag ggtaccgcag
     1621 aactggcgaa cctgggaaag atcacatcga ccgtcgggcg ctaccgcccc gataacctgc
     1681 gatatccaaa agcgttgaag gatatgcgcg ctaaagactg gcgcgcctac ggccagcagt
     1741 acatcgagga aacacggcga catcgcctga cggacaagcc gttctttacg gacaaattac
     1801 cgaacaattt cccgcacgtt ggattcctgc acctggtact gccgcgggcg aaaatcatca
     1861 atgcgcgccg ccacccgctc gacagttgcc tcggtggcta caagcagctg ttcggcaagg
     1921 ggcagaactt cacgtatgac atgttcgagc tcgccgatta ttacaggcaa tatcacaaca
     1981 cgatgcagca ctggcacagg gtgttgccgg gaaaggtcct cgacgtgcac tacgaggaaa
     2041 cggtgatgga tctcgagggc caggtaaggc gaattctgga gcactgcggc ctgccgttcg
     2101 aggaggcttg cgtccgctac tacgaaacgg cacgagccgt gcagaccgcg agctcggagc
     2161 aggtacgcca gccgatctac cgggaaggcc tcggcaagtg gcgccgttac gagcagtacc
     2221 tcgacctttg gaaagaggag cttgcggaca ttctcgacga gcttcccgac acggtgagga
     2281 acgcaggact gcaggattca tgaacgcccg gtttgcttga gcgctgcggc aggaacacct
     2341 tcaagcgtac ggccacacct ccaggccgtg gatggcgttg cggatgtcca tggtcaggtt
     2401 gacggacagc agcctttcgt tggaggggag atcgtatacc gtgatcgtcg agggtgacga
     2461 tccacccgca acgaccgcgt cggacaggac gcaaaggcca cgcgcgaatc cctgccgcgc
     2521 gagccgggcg gagtcctcgc tcacgtgtgt cagcaaatcc tgcggatatg ccgggaccct
     2581 gaaagcgcga tcctcgccgc cttcgccgcg gccggcgtaa cgcagcgcat tcgcctcgct
     2641 gtcgttgaac agtacaccgt cgcggaaagg gcgggcgttg tggctgccgg gcggcagttc
     2701 cgccgacatg tggatagcct tgccgttgaa gtgcaacatg ccgccggttc gcaggccacc
     2761 tatgtacatg ccttcggggt tgcacacgac attgttgatg tgcagcttgt tcaacatgag
     2821 cgggccatcg gtgccgcggg gatcgtagcg caagccgcgg aacctgaact cgttggtcgc
     2881 gatgtgcagg ccccactcga actctcgact gtcgaggttg aatcccagga cgctgtcgaa
     2941 gccggtcgag gtcaggtaaa gggtgcgttt ataaaccgcg atctcgtgac agtgcttgag
     3001 gtagcggttg cgccagctcc cgatcctctc gaagtccggc gtgtaggcga agagttcatc
     3061 gctggccgcg atgtagagcg tctccccgtc gaacgcgatg ccacgtagac cacgatccca
     3121 gccgcggcca tgccagtcta tgtcggcggt gttccaatcg agcgtctggc ggacggtccg
     3181 atcgtcgagg tcgatcagat agacgccgcc gtgactttct ccctgatggc tgccgcggac
     3241 tacggaggtc gtgacgagtt tcagaagctc tttcgcaccg tcaggctgaa ggtccgcggc
     3301 ttctgcaaag aatgcgactc gttgaagcgc agatcgtaga acacaaggga atcgccatag
     3361 ttgaggttac tcaaccagct tacgttcttc tcgtcccaga cattgcgcac catgagagag
     3421 atatcccact cgttctccat ttctacccct acctggaaat tcgcggtggt ggaggacggt
     3481 atgatgccg
//
LOCUS       DASUOM010000387        10318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1282229,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000387 DASUOM010000000
VERSION     DASUOM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282229"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1294
                     /locus_tag="VFY27_08040"
     CDS             233..1294
                     /locus_tag="VFY27_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5765504.1"
                     /translation="MLFRRKSQPLLGLDITTSSVKLIELSQSGKRYRVESYSAEPTPP
                     SSVNEKAIVDAKAVGEAIRRAVKRSGAKATDVAVAISGDAAITKVIQMPNSLSERDLE
                     GQVEIQADQYIPFPMEEVSFDYEIVGPNANDPELMDVLLVATRTDNVEQRQAAVQAAG
                     LHAQIVDVEAFALENACQLLSHQIPDNGIGQSVAIVDIGASSTTFSVLKDLKVIYTRD
                     FAFGGQQLTEEIMRTYGLSMEDAGRAKKQGGLPSNYQPEVLEPFVDDVTQQVSRSLQF
                     YLASGGGREQPDMILVCGGCANIPGIADVISSRVGIPTEVGDPLGQMKISSKAKSQGV
                     HKDSTALLTACGLALRSFD"
     gene            1296..1874
                     /locus_tag="VFY27_08045"
     CDS             1296..1874
                     /locus_tag="VFY27_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5765505.1"
                     /translation="MPRINLLPWREEERKKRQRDFLVATGGAFVAAIAVVGLTMFFYG
                     QMIDGQKARNSRLQAEIAELDKSIKEIDGLEAQKARLLARMEIIEQLQRSRPEIVHLF
                     DEVTRQLPEGVYLTGMKQNGVQVEVTGVAQSSTRVSALMRQIDRSEWLTDPDVVKVET
                     TTEGPARQASFIVTLKQVRHGDDADAEMEDAG"
     gene            1871..2470
                     /locus_tag="VFY27_08050"
     CDS             1871..2470
                     /locus_tag="VFY27_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5765506.1"
                     /translation="MSLVDELRSLDVNDIGRWPLVFRVAVIGIVFAVVLGFGIYWTIV
                     EDRAPQLKRAQDEEQTLRVAFENKQRKAANYDEYKKQLEQMEQSFGTMLRQLPGETEI
                     PSLIVDISQTGLAAGLQEKLFQPQPEVPKDFYAEKPILIHLTGSYHEIANFVSGIAAL
                     PRIVTLHNINVKPDSTESFDRLSIEVTAKTYRYLEDAQL"
     gene            2467..3006
                     /locus_tag="VFY27_08055"
     CDS             2467..3006
                     /locus_tag="VFY27_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX5765507.1"
                     /translation="MRAAALTSIMRKALLPVVFAGAVLAGCSGDMDDLDSYINEVKAR
                     PGGRIEPLPEITPYEVFTYVADTEGLRSPFVPDTQQSAAGPSGGARPDLERSREFLEQ
                     FPLDSLNMVGTLEIGTATFGLVQTRDGLIHRVSPGNYVGQNDGRVTAVTESEIQVLEI
                     VSDGIGGYLERDAAIGLSD"
     gene            3035..5245
                     /gene="pilQ"
                     /locus_tag="VFY27_08060"
     CDS             3035..5245
                     /gene="pilQ"
                     /locus_tag="VFY27_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX5765508.1"
                     /translation="MTLKIKPEVSATAAQGVRNRRLGKQLVLAAQAVVFMLFGLSATA
                     QEGNRLQDIQVQSLPGQRVELRLITSGTAPEPLAFTIEDPARIALDLPETALGMASRR
                     RDINLGPLDTVLTAEASGRTRVVLNLDSMVPYETRRSGNTIYVTLGQAGAVASGTTQF
                     EGAASPDSRSYATSGARSINNIDFRRTGDGGGRVMVTLTDPSTAVDIREEGGRVVAVF
                     KDTTLPSELMRRLDVMDFATPVTTVDALRTNSDTRIVISAQGSYEQLAYQSDNEFTIE
                     INPTAEDEEQQASLFSETKEYTGQRLTLNFQDIETRAVLQLLAETSGRNIVVSDTVQG
                     NVTLRLRNVPWDQALDIVMTTKGLDMRQNGNVIIVAPAEEIAARETADLEAKRAITEL
                     EPLYSEFLQVNYAKAANLAALIASTEGQNSLLSERGTVSIDDRTNTLLVQDTAEKLQD
                     IRRLVRTLDIPIRQVLIESRIVIVNDDLSRDLGVRLGLTAYNQNGDDGVTVISGTGTG
                     TDTMINSALDNLGDPNNGTIFPIQLPDLANRYNVNVPIQNAAGRFALAVLESDYLVDL
                     ELTALEAEGRGEIVSTPRVITANQKEARIEQGVEIPYQESASSGATTTQFKKAVLSLT
                     VTPQITPDNNIIMDLLVSNDTVGDLVASATGGFVPSIDTRAVETQVLVADGQTVVLGG
                     IYETERRETITKVPVLGDIPGVGNLFKSRQNVSNKAELLIFVTPKILTEGSSIY"
     gene            5415..5945
                     /gene="aroK"
                     /locus_tag="VFY27_08065"
     CDS             5415..5945
                     /gene="aroK"
                     /locus_tag="VFY27_08065"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEX5765509.1"
                     /translation="MKNPNNLFLVGPMGAGKSAVGRQLSRMLHLDFVDSDDEIESRTG
                     VDIPFIFEKEGEEGFRAREARVIDDLSQLEGIVLATGGGAVLDANSRNHLGARGFVIY
                     LYTSVRQQLERTNRGRERPLLGGGDKAEVLERLMAERDPLYREIADLVINTDGRRVQT
                     VAREIQQALTENSGAP"
     gene            6123..7211
                     /gene="aroB"
                     /locus_tag="VFY27_08070"
     CDS             6123..7211
                     /gene="aroB"
                     /locus_tag="VFY27_08070"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5765510.1"
                     /translation="MAARIITVDLADRSYPIVIGRNLLDGNYDLAAQLPGSDCLVVSN
                     ENVAPLYLERLEKNLPGCNVEAMLLPDGEAWKTLESAAAILDRLVALKARRDTTVMAL
                     GGGVVGDIAGFAAACYMRGVAFVQVPTTLLAQVDSSVGGKTGVNHAGGKNLIGAFHQP
                     RLVLIDTDTLASLPEREFRAGLAEVIKHGAIADAGFFSWLEQNIRGLLAKDPEALAHA
                     IGRSCEIKASVVSEDETEQGRRALLNFGHTFAHAIEKTLGYGEWLHGEAVAAGMVMAA
                     QLSNIDQGDRDRLQLLLDAAGLPVAPPPRSGDRLRAAMQLDKKARAGQLRFVLLDRLG
                     EARLSSGIPEDRLQDILEGGKPATASTP"
     gene            7208..8362
                     /locus_tag="VFY27_08075"
     CDS             7208..8362
                     /locus_tag="VFY27_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX5765511.1"
                     /translation="MTNLVEEVTLAPYAADETRGKGRRIPEAAARYRGEYQRDRDRII
                     HSTAFRRLVYKTQVFVNHEGDLYRTRLTHSLEVAQIARTVARALRLNEVLVEAICLAH
                     DLGHTPFGHAGQDSLNDCMQDFGGFEHNLQSLRVVDELEHKYADFPGLNLTFETREGI
                     LKHCSARNARELGELGRRFLERHQPGLEAQIANVADAIAYNNHDVDDGFRANLLDLEQ
                     LREQELFGKQYQTVIALYPGLDDRRLIYEIIRRMIDTLVTDLIESTRSELDRLRPKSI
                     EEVRAQPAPVVRFSEEVFEQHTRLKQFLNRNLYRHGKVRLMTDKARVTVRELFDRYMG
                     SPGEMAPEFARKAQTPGADEARRARVVADYVAGMTDRFAIAEHDRLTRLL"
     gene            8483..9193
                     /gene="pyrF"
                     /locus_tag="VFY27_08080"
     CDS             8483..9193
                     /gene="pyrF"
                     /locus_tag="VFY27_08080"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5765512.1"
                     /translation="MNYPTPAPGERLIFAMDVADCDHARRLAEELGESVTFYKLGLEL
                     MMSGGYFDLVEWMLQRDKKVFVDLKFFDIPATVGSAVRQLKDRGVSFVTVHGNQSIME
                     AAAENKGDELKVLAVTVLTSLDRGDLDDLGFDCNVEDLVLSRARRAFEAGCDGVISSG
                     LEVARLRQQVDRRLVVVTPGIRPVENQPEGDQKRVVSVESAFESGADYIVVGRPIRDA
                     ASPREAAEAIQSTIKSLF"
     gene            9200..10270
                     /gene="hemE"
                     /locus_tag="VFY27_08085"
     CDS             9200..10270
                     /gene="hemE"
                     /locus_tag="VFY27_08085"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5765513.1"
                     /translation="MSPPLKNDVLIRALLRQPVPYTPVWIMRQAGRYLPEYREVREKA
                     GDFMSLCRNPELACEVTLQPLRRFDLDAAILFSDILTVPDAMDLGLYFVTGEGPKFER
                     PVTRSSDIRKLRIPDVSKKLRYVFDAASLVRRTLEGRVPLIGFAGSPWTVATYMIEGH
                     STREFAKAKGLMAEDPKSLQQLLDVVATTTTEYLNAQIESGAQAVMIFDTWGGVLERE
                     QYRRFSLHYMQQILDGLKRNHDGAQIPVILFTKGAGKLLEDMAQTGCDALGVDWTTDL
                     AEARQLTSDRVALQGNLDPATMREAPSTIEEGVLRVLESYGEGPGHVFNLGHGITPDI
                     DPDRVAVLVDAVHRLSPRFHTA"
BASE COUNT         2191 a         3191 c         3207 g         1729 t
ORIGIN      
        1 gagagaagaa tcctcaacgg gtcgggcaga ttcgggatat gggggagaac tgtctcttac
       61 cagcgccaac cagttcacgg aatcagggga agtcccggca gtaccatagg cgccgcggat
      121 cgtgaacaaa gtcacgtttt atttaacatt ttgttgatat atagttaaat cgccttgctc
      181 aggtccaacc taaaatagac ccgcaactta ccgagcttac aaggaaaaac acgtgctttt
      241 ccgcagaaaa tctcagccat tgttgggcct cgacatcacc acgtcttcgg taaagctcat
      301 agagctgtcc cagagtggta aacgctaccg ggtcgagtcg tattccgcag agccaacccc
      361 gccgagctcg gtgaacgaga aggcgatcgt cgacgccaag gccgtcggtg aggcgatacg
      421 ccgcgccgtg aagcgctcgg gcgccaaggc cacggacgtt gcggttgcga tcagcggtga
      481 tgccgcgatc accaaggtca tacagatgcc gaacagcctg tccgaacggg atctcgaggg
      541 ccaggtggag atccaggcag accagtacat acccttcccg atggaagaag tcagcttcga
      601 ctacgaaatt gtcggaccga acgcaaacga tccggaactg atggacgtgc tgctggtggc
      661 cacccgcact gataatgtcg aacagcgcca ggcggcggtc caggcggcgg ggctgcatgc
      721 gcagatcgtc gacgtagaag cgttcgcgct ggagaacgcc tgccagctac tgagtcacca
      781 gattccggac aacggcatcg gccagtccgt cgccatcgtc gatatcggcg ccagcagcac
      841 gacattcagc gtgctgaaag accttaaggt catctatacg cgcgacttcg ccttcggcgg
      901 ccagcagcta accgaggaaa tcatgcgtac ctacggcctc agcatggaag atgcgggccg
      961 tgccaagaag cagggcggct tgccgagcaa ctaccagccc gaagtgctgg agccgttcgt
     1021 ggacgacgtc acccagcagg tgagccgttc cctccagttt tacctggcat ccggcggcgg
     1081 acgcgaacag cccgacatga tcctggtctg cggcggatgc gcgaacattc ccggtattgc
     1141 ggacgtcatc tcgagccgcg tgggtatccc gacggaagtc ggcgaccccc tggggcaaat
     1201 gaaaatatct tcgaaggcga aatctcaggg cgtgcacaag gactccactg cgctcctgac
     1261 ggcctgcgga cttgcactga ggagcttcga ctaacatgcc acgcattaac ctcctccctt
     1321 ggcgcgaaga ggaaagaaag aaacgacagc gcgatttcct cgtcgccacg ggtggcgctt
     1381 tcgttgcggc gatcgcggtc gtcggcctga ccatgttttt ctatggccag atgatcgacg
     1441 ggcagaaggc tcgtaactcc aggctgcagg cggagatcgc cgagctcgac aagagcatca
     1501 aggaaatcga tggcctggaa gcgcagaagg cgcgacttct ggcacgcatg gaaatcatcg
     1561 agcagctgca gcgcagccgc ccggagatcg tgcacctctt cgacgaggtg acccgccagc
     1621 tgccggaagg tgtttacctg acggggatga agcagaacgg cgttcaggtc gaagtcaccg
     1681 gcgttgcaca gtccagcacg cgcgtgtccg cgctgatgcg gcagatcgac cgctccgaat
     1741 ggctgacgga tccggatgtg gtgaaagtcg agacaaccac cgagggaccg gcgcgccagg
     1801 caagtttcat cgtgacgctg aagcaagtga ggcatggcga cgacgctgac gccgaaatgg
     1861 aggacgcggg atgagcctgg tcgacgaact ccgaagtctc gacgtcaacg atatcggccg
     1921 ctggccgctg gtattccggg ttgcggtcat cggcatcgtc tttgccgtcg tactgggctt
     1981 cggcatctac tggacgatcg tcgaggatcg cgcgccgcaa ctgaagcgtg cccaggacga
     2041 agagcagaca ctgcgagttg cgttcgagaa caagcagagg aaggcagcga actacgacga
     2101 gtacaagaag cagctcgagc agatggagca atccttcggc acgatgctgc ggcagctgcc
     2161 cggcgaaacg gagataccga gtcttatcgt tgacatttcc cagaccgggt tggccgcggg
     2221 cttgcaggag aaactgttcc agccgcagcc agaggtaccg aaggatttct acgcagagaa
     2281 gccgattctc attcacctca cgggcagtta tcacgaaatt gcgaatttcg tcagcggcat
     2341 cgcagcgctg ccgcgaatcg tcaccttgca caacatcaac gtgaagccgg acagcaccga
     2401 gagctttgat cggctgtcga tcgaggttac ggcgaagacc tatcgttacc tggaggacgc
     2461 gcagttatga gagctgctgc cctgacatcg atcatgagaa aagcactgct gccggtcgtc
     2521 tttgccggcg ccgtcctggc aggctgttcc ggtgacatgg acgacctgga cagctacatc
     2581 aacgaagtga aggcgcgtcc cggcggccgc attgagccgc tgcccgagat cacgccgtat
     2641 gaagtgttca cttatgtcgc ggacacggaa ggcctgcgct cgccgttcgt gcccgacacg
     2701 cagcagagcg cggccggacc ctctggcggc gcacggccgg acctcgagcg cagccgtgag
     2761 ttcctggagc agttcccgct ggattcgctg aacatggtcg gcaccctgga gatcggcact
     2821 gccacgttcg gcctggtaca gacgagggac ggcctgattc accgggtatc gccgggcaac
     2881 tatgtcggcc agaacgacgg ccgggttaca gcggtgacgg aatctgaaat tcaagtgtta
     2941 gagattgtgt cggacggaat cggcggctat ctggaacgcg acgccgctat cggtctttcg
     3001 gactgaggcc tgaatggaac tgggagtatt gggaatgacg ctcaaaataa agccggaagt
     3061 gtcggcgaca gccgcacaag gcgtacgaaa ccggcgtttg gggaagcagc tggtcctggc
     3121 ggcgcaggcc gtagttttca tgttgttcgg cctgagcgct accgcccagg aaggcaaccg
     3181 gctgcaggat atccaggtgc agagcctccc gggccagcgc gtcgaactgc gcctgatcac
     3241 gagcggaacg gctcctgagc cacttgcatt caccatcgaa gacccggcgc gcatcgcgct
     3301 cgacctgccg gaaaccgcac tcggcatggc ctcgcggcgc cgtgacatca atctcggccc
     3361 cctggacacg gtgctgaccg cggaggcaag cggccgcacc cgcgtcgtgc tgaacctcga
     3421 ttcgatggtg ccttatgaga cccgtcgcag cggcaacacg atctacgtga cgctgggaca
     3481 ggctggcgcc gtcgcttccg gcaccacgca gttcgaaggc gctgcatcac ccgattcgcg
     3541 ttcctatgcg accagtggcg cgcgctccat caacaacatc gattttcgcc gcaccggcga
     3601 cggcggcggc cgcgtgatgg tcacgctgac cgatccctcg acggctgtcg acatccgcga
     3661 ggagggcggc cgtgtcgttg ccgtattcaa ggacacgacc ctgccttcgg aactgatgcg
     3721 tcgcctggac gtcatggatt tcgctactcc ggtaacgacc gttgacgccc tgcgcaccaa
     3781 ctcggacacc cgcatcgtga tctcggccca gggcagctac gagcagcttg cctaccagtc
     3841 cgacaacgaa tttacgatcg aaatcaaccc gactgcagag gacgaggagc aacaggcgag
     3901 cctgttctcc gaaaccaagg agtacacggg acagcgcctg acgctgaact tccaggacat
     3961 cgagacccgg gcggtactgc agttgctggc ggaaacgtcg ggccgcaaca tcgtggtctc
     4021 cgacaccgtg cagggtaacg tgaccctgcg cctgaggaac gtaccctggg accaggctct
     4081 cgacatcgtc atgacgacca agggcctgga catgcggcag aacggcaacg tgatcatcgt
     4141 cgcgcccgcc gaagaaatcg ccgctcgtga aaccgccgac ctggaagcga agcgagccat
     4201 caccgagctc gagccgctgt actcggagtt cctgcaggtc aattatgcaa aagcggcgaa
     4261 tcttgcggcg ctcatcgcgt cgacagaagg gcagaattca ctgctgtcgg aacgcggtac
     4321 ggtatcgatt gacgaccgca ccaataccct gctggtacag gacacggccg agaagctcca
     4381 ggacatccgc cgcctcgtgc gcaccctgga cattccgatc cgccaggtcc tgatcgaatc
     4441 gcgcatcgtg atcgtcaacg acgacctgag ccgtgacttg ggcgtccgcc tgggcctgac
     4501 ggcttacaac caaaacggcg atgacggcgt aacggtcatc tccggaaccg gcaccggcac
     4561 cgacacgatg atcaactctg cgctcgacaa cctgggcgac ccgaacaatg gcacgatctt
     4621 cccgatccag ctgccggacc tcgcgaaccg ctacaacgtg aacgtgccga tccagaacgc
     4681 ggcagggcgc ttcgcgctgg ctgttctcga atcggattac ctggtcgacc tcgaactgac
     4741 tgcgctggaa gccgagggac ggggcgagat cgtatcgacg cctcgcgtca tcacggcgaa
     4801 ccagaaggaa gcgcgtatcg agcagggtgt cgagattccg taccaggaat cggcgtcttc
     4861 cggagcgacg accacgcagt tcaagaaggc ggtcttgagc ctcaccgtta cgccgcagat
     4921 cacgccggac aacaacatca tcatggatct gctggtgagt aacgacaccg tgggcgacct
     4981 cgtggcgagc gcaacgggcg gtttcgttcc gagcatcgac acgcgggctg tcgaaacgca
     5041 ggtcctggtc gcagacggcc agaccgtggt actcggcggc atctatgaga ctgaacgccg
     5101 cgaaacgatc accaaggtac cggtgcttgg cgacataccc ggagtaggca acctgttcaa
     5161 atcgcgccag aacgtatcga acaaggcaga actgctgatc ttcgtgacac ccaagatcct
     5221 gacggaaggc tcgagcatct actaagaaag aaagttcgcg cagaacccgg caggatgtag
     5281 gaccccgcca ccatgcgggc gagtgcgatt gacattggcg tcgcccgcat ggcgggctcc
     5341 tgcggtgttc cggtcggggc ttgcgacgag ctcccgggat catcagggca gcccggaatt
     5401 cactgaacag aaaaatgaag aacccaaaca acctgttcct ggtaggcccc atgggtgccg
     5461 gcaagtcggc tgtcggccgg cagctgtccc ggatgctgca tctcgacttt gtcgacagcg
     5521 atgacgagat agaaagtcga accggcgtcg acatcccgtt catcttcgag aaggagggcg
     5581 aggaaggctt tcgcgcccgc gaagcccgcg tcatcgacga cctgtcgcag ctggagggca
     5641 tcgtgcttgc gaccggcggc ggagccgtgc tcgatgcgaa cagccgcaat cacctcggtg
     5701 cacgcggctt cgtgatctac ttgtacacca gcgtgcggca gcaactcgaa cgcaccaacc
     5761 gcggacgcga gcgaccgctc ctgggaggcg gggacaaggc agaggtcctg gaacggctga
     5821 tggcggagcg ggatcccctg taccgcgaga tagccgacct ggtgatcaac accgacggaa
     5881 gacgggtgca gacggtcgcc cgcgagatcc agcaagccct caccgagaac tccggcgcgc
     5941 cgtagagccc gccatgcggg cgagtgcgac agactcagcg agttcgcccg cattcgccgt
     6001 ccccgagacc aacagggttc gatgtcgttt gtagttccct tgggcgttag ctcctgcagt
     6061 tgccccaccg ctctccacga tcgggtaacc tatcccgcct gatctctccc gagtccagcc
     6121 gcttggcagc aagaatcata acggtcgacc tggccgatcg cagttacccg atcgtcatag
     6181 gcaggaacct gctggacggc aattacgacc ttgcggcgca gctgccaggc tcagactgcc
     6241 tcgtcgtcag caatgagaat gttgcgcctc tgtacctgga gcggctggag aaaaacctgc
     6301 cgggctgcaa cgtcgaggcg atgctgctgc cagacggcga ggcctggaag acgctggaaa
     6361 gtgcggctgc catcctggac cggctggtcg cactcaaagc gcggcgcgac acgaccgtga
     6421 tggcactcgg cggtggcgta gtgggtgaca tcgcaggatt cgccgcggcc tgctacatgc
     6481 gcggcgtcgc gttcgtgcaa gtgccgacga ccttgcttgc gcaggtagat tcctccgtcg
     6541 gcggcaagac cggtgtcaat catgccggcg gcaagaacct catcggggca ttccaccagc
     6601 cgcggctggt cctgatagac accgataccc tggccagcct gccggaacgg gaatttcgcg
     6661 cggggctcgc cgaagtgata aagcacggcg caatagcaga cgccggtttt ttttcctggc
     6721 tggaacagaa cattcgcgga ctcctggcca aggaccctga agcactggca catgccatcg
     6781 gccgctcttg tgagataaag gcctcggtcg tttccgagga cgaaacggaa caagggcgcc
     6841 gagcgctgct gaacttcggc catacgttcg cgcacgctat cgagaagacc cttggatacg
     6901 gtgagtggct gcatggcgaa gccgttgccg ccggaatggt aatggctgcg caattgagca
     6961 acatcgacca gggagaccgg gaccgcctgc agctcctcct ggacgcagcg gggctgccgg
     7021 tggcaccgcc gccgcgaagc ggtgaccgct tgcgcgccgc aatgcaactc gacaagaaag
     7081 cgcgcgcggg gcagctgcgc ttcgtcttgc tcgaccgcct gggggaagca cgcctcagca
     7141 gcggaatacc ggaggaccgg ctgcaggaca tcctcgaagg ggggaaaccg gcgacggcaa
     7201 gcacaccatg acgaatctcg tggaagaggt gacgctcgcc ccttatgccg cggacgagac
     7261 gcgcggcaag ggcaggcgca ttccggaagc ggccgcacgc tatcgcggcg agtaccagcg
     7321 cgaccgcgac aggatcattc attccaccgc gttccggcgc ctcgtttaca agacccaggt
     7381 cttcgtcaat cacgagggcg acctctaccg cacgcggctt acgcattccc tggaagtcgc
     7441 ccagatcgca agaacagtcg cgcgcgcctt gcggttgaac gaggtgctgg tcgaggcaat
     7501 ctgcctggcg cacgacctgg ggcacacgcc gttcggccat gccggacagg attcgctgaa
     7561 cgactgcatg caggatttcg gcggtttcga gcacaacctg caatccctgc gggtcgttga
     7621 cgagctggag cacaagtacg cggactttcc cgggctgaac ctcacgttcg agacccgcga
     7681 aggcattctc aagcattgct cggcacgcaa cgcccgcgaa ctcggcgaac tcggcaggcg
     7741 tttcctcgag cgccaccagc cgggcctcga ggcacagatc gcgaacgtgg cggacgcgat
     7801 tgcctacaac aaccatgacg tcgacgacgg cttccgggca aacctcctcg acctcgaaca
     7861 actgcgggaa caggagctct tcggcaaaca ataccagaca gtgattgccc tctatcccgg
     7921 cctcgacgac cgccgcctga tctacgagat catccgccgc atgatcgaca cactggtcac
     7981 cgacctgatc gagagcacgc gttcggaact cgaccgcctg agaccgaagt ccatcgaaga
     8041 agtcagggcc cagcccgccc ctgtcgtccg cttcagtgaa gaggtcttcg agcagcacac
     8101 ccggttgaag caattcctga atcgcaacct ctaccggcac gggaaggtgc ggctcatgac
     8161 ggacaaggca agggtcaccg tgcgcgagct gttcgaccgc tatatgggct cgccggggga
     8221 gatggcgccc gagttcgcgc gaaaggcaca aacaccaggt gccgacgaag cccgcagggc
     8281 tcgcgtggta gccgactacg tcgcgggaat gaccgaccgc ttcgcgatcg ccgagcacga
     8341 ccggctgacc cgcctgctgt aagaacccgg catcgccgga cgagttcgcc aatcccagct
     8401 gttagaatgc gcggctgcaa cttcattccc atggcaagcg tccccaggtt gcctccagga
     8461 tcagcgcagg agacacgata tcgtgaacta cccgactccc gctccgggcg agcgcctgat
     8521 attcgccatg gatgtggcgg attgcgatca cgcgcggcgg ctcgccgaag agcttggcga
     8581 gtcggtgacc ttctacaagc tcggcctcga gctgatgatg agcggcggat atttcgatct
     8641 cgtcgagtgg atgctgcagc gggacaagaa ggttttcgtg gacctcaagt tcttcgatat
     8701 ccccgcgacc gtcggatccg cggtgcggca gttgaaggac aggggcgtaa gtttcgttac
     8761 cgtgcacgga aaccagtcga tcatggaagc cgcggcggag aataagggcg atgaactcaa
     8821 ggtgctggcg gtaacggtcc tgaccagcct cgaccgcggt gacctcgatg accttggttt
     8881 cgactgcaac gtcgaggacc tggtgctgtc gcgcgccagg cgggccttcg aggcgggatg
     8941 cgacggcgtc atctcttccg ggctcgaagt tgcaagacta cgtcaacagg tcgatcgccg
     9001 gctggttgtc gtcactcctg gcatccggcc cgtcgaaaac cagccggaag gcgaccagaa
     9061 gagagtggtg tccgtcgaga gcgcgttcga aagcggcgcc gactatatcg tcgtcggccg
     9121 cccgatccgc gacgcggcat cgccgagaga agccgcagag gctatccagt caacaatcaa
     9181 gtccttgttt tagaagaaca tgtcgccgcc actcaagaac gacgtgctga ttcgcgcact
     9241 gctgcgacaa ccggtgccgt acacgccggt ctggatcatg cgccaggcgg gcaggtacct
     9301 gccggagtat cgcgaggtcc gcgagaaggc cggcgatttc atgagcctct gccgcaaccc
     9361 ggaactggcc tgcgaggtaa cgctgcagcc gctaaggcgc ttcgatctcg acgcggcgat
     9421 cctcttctcc gacatcctta ccgtccccga tgcgatggac ctcggccttt acttcgtgac
     9481 cggtgaaggc cctaaattcg aaaggccggt cacgcgctca tcggacatcc gcaagcttcg
     9541 gattcccgat gtcagcaaga aactgcgcta cgttttcgac gcggcatcgc tggttcgacg
     9601 cacgctggaa ggcagggtgc cgctcatcgg cttcgccggc agtccctgga cggtggcgac
     9661 ctacatgata gaaggccatt caacgcggga gttcgccaag gcaaaggggc tgatggccga
     9721 ggatccgaag tcgctgcagc agttgctcga tgtcgtcgca accaccacca cggagtacct
     9781 gaatgcgcag atcgaatccg gcgcacaagc ggtcatgatc ttcgacacct ggggaggcgt
     9841 gctggagcga gagcagtatc gccgcttctc gctgcactat atgcagcaga tcctcgacgg
     9901 cctgaagcgc aatcacgacg gcgcgcaaat cccggtaata ctgttcacca agggagcggg
     9961 caagctcctc gaagacatgg cgcagaccgg ctgcgacgcg ctcggcgtcg actggaccac
    10021 cgacctcgca gaagcacgcc agctgacaag cgatcgcgtc gccttgcaag gcaacctcga
    10081 cccggcaacg atgcgtgaag cgccgtcaac gatcgaagag ggcgtcctgc gcgtactcga
    10141 gagctatggc gagggccccg gccacgtctt caacctgggc cacggcatca cccccgacat
    10201 cgaccccgac cgcgtcgcag tactggtgga cgcagtccac cgcctgagcc cccgcttcca
    10261 caccgcgtag gcccggaatc acgaaccggc acgactcgga cgagggagtt ctggagag
//
LOCUS       DASUOM010000388         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_613034,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000388 DASUOM010000000
VERSION     DASUOM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613034"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFY27_08090"
     CDS             <1..1232
                     /locus_tag="VFY27_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5765514.1"
                     /translation="HGNIRFIGIDLNEDDYEGYYNGFSNKVLWPLFHYLLSFIEYKRA
                     DFHAYLRVNTMFARKLIPLLEPDDVIWVHDYHLIPLASELRKAGIRQPIGFFLHVPFP
                     DFDVLQVLPDRNFILRAMCAYDVVGFHTHRDLASFRAAVSEPEIGAEVLGDGTIRVGD
                     RLVRAGAFPIGIDVDTLVSFAEDAQNDSEVIRTRHGLEGRRFIIGVDRLDYSKGLEQR
                     FRSYARLLEKYPETRGTVSYTQIAPPTRVGVRTYDDIRTSLEQTSGEINGRFATLDWV
                     PIKYINRGTNRKALMGLFRLANVGLVTPVRDGMNLVAKEFVAAQDPENPGMLVLSTLA
                     GASHELKDAVLVNPYDTDDVADGINRALTMSLDERRYRHAQMMTALRKNDIVAWRDRF
                     IEALCAPKEGNGDEPAS"
     gene            1210..2163
                     /gene="glk"
                     /locus_tag="VFY27_08095"
     CDS             1210..2163
                     /gene="glk"
                     /locus_tag="VFY27_08095"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX5765515.1"
                     /translation="MATNLQADATWLIADIGATSTRCALCRIGDKRHSELQIFLNEKF
                     ESLKALLQDYVSLRNDKPSAGALAIAAPISGDDVQMTNRNWSFNRLEMAEALGLARLD
                     IINDFHAVAYALPQLPKRDVVEIGKATTYRNGTRASLGPGSGLGVGAWIAGNGKGVAM
                     YGEGGHVSLPARSEAEERIIAKIRERYGHASAERVLSGPGIIALHSAMHGENMKTSRE
                     ITSGTVDRKCRATMEQFYRFLATVAADLAMTTGAYGGVFIAGGIVPDNIEQIQASTFR
                     ERFEDKNRYRHYMQAIPTYVVTDPTPGLTGLMAYIVEHQSG"
     gene            complement(2198..2425)
                     /locus_tag="VFY27_08100"
     CDS             complement(2198..2425)
                     /locus_tag="VFY27_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765516.1"
                     /translation="MIKTLYLVTVVLCLAGCNDSREPAVTGTPEEQGVFDPMTDQLEK
                     ARNVEAAAMQHKEDIDKALERSDTTPVDDHE"
     gene            complement(2452..2973)
                     /locus_tag="VFY27_08105"
     CDS             complement(2452..2973)
                     /locus_tag="VFY27_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5765517.1"
                     /translation="MTMPAVFFSHGQESGPWGTKIKAMAETARELGCRVESVDYQGID
                     DPTERVAKLVRTCASVEEPLILVGSSMGGHVATAAASAVGAIGVFVLAPAYYMQGYEH
                     LTPPAPDIPLAIVHGWRDDVVPVENSIRYARGCLATLHIVNGDHRLSANINEIDSYLR
                     QFVVDLAAALAPD"
BASE COUNT          585 a          995 c          905 g          585 t
ORIGIN      
        1 agcatggcaa catccgcttc atcggcatcg acctgaacga agacgactac gagggctact
       61 acaacggctt cagcaacaag gtcctctggc cgttgttcca ttacctgctg agcttcatcg
      121 aatacaagcg ggcggatttc cacgcgtacc tgcgggtcaa caccatgttc gcacggaagc
      181 tgattcccct cctggaaccc gacgacgtga tctgggttca cgactatcac ctgatcccgc
      241 tggcctccga actgagaaag gccgggatcc gccagccgat cggattcttc ctccacgtgc
      301 ccttccccga tttcgacgtc ctgcaggtgc tgccggaccg caacttcatc ctgcgcgcga
      361 tgtgcgcgta cgacgtcgtc gggttccaca cccatcgcga ccttgcctcc ttccgtgccg
      421 ccgtctccga gcccgaaatc ggcgcggagg tgctgggcga cggaaccatt cgcgtcggcg
      481 accgcctcgt gcgcgccggc gccttcccga taggcatcga cgtcgacacg ctggtctcgt
      541 tcgctgaaga tgcgcagaac gactcggagg tgattcgcac ccgccacggg ctcgaagggc
      601 ggcgcttcat catcggggtc gaccgcctcg actacagcaa ggggctggag cagcggtttc
      661 gctcttatgc caggctgctg gagaagtatc cggagacccg cggcaccgtg tcctacacgc
      721 agatagcgcc gccgacccgc gtcggtgtca ggacttacga cgacatccgc accagcctcg
      781 agcagacgtc gggcgagatc aatggcaggt tcgctacgct ggactgggtt cccatcaagt
      841 acatcaatcg cggcaccaat cgcaaagcgc tgatgggtct gttccggctg gccaatgtcg
      901 gtctcgtcac acccgtccgg gacggcatga atctcgtggc gaaggagttc gttgcggcgc
      961 aggatcccga aaatcccggc atgctggtgt tgtccacgct ggccggcgcc tcgcatgaac
     1021 tcaaggatgc ggtgctggta aacccatacg ataccgatga tgtcgccgat ggcatcaaca
     1081 gggcgctgac tatgtcgctc gatgagcgcc ggtaccggca cgcgcagatg atgacggcgc
     1141 tgcgtaaaaa cgatatcgtg gcatggcgcg atcgattcat cgaagcatta tgtgcaccga
     1201 aagaagggaa tggcgacgaa cctgcaagct gacgcaacct ggcttattgc cgatatcggc
     1261 gcgacctcga ctcgctgtgc gctctgtcgc atcggggaca agcgccatag cgaactgcag
     1321 atcttcctga acgaaaagtt cgaatcgctg aaggctctgc tacaggacta cgtctctctg
     1381 aggaacgaca aaccttccgc gggcgcgctc gccattgcgg ctcccatctc aggcgacgac
     1441 gtgcagatga ccaatcgcaa ctggagtttc aaccggctgg agatggctga agctctcggg
     1501 ctcgcgcgcc tcgacatcat caacgatttc catgcggtcg cctatgcgct gccgcagcta
     1561 ccgaaacggg acgtggtgga gattggcaaa gccaccacct atcgcaacgg cacccgcgca
     1621 tctttgggac ccggcagcgg cctcggggtc ggcgcgtgga tcgccggtaa cggcaagggc
     1681 gtcgcgatgt atggcgaagg cggtcatgta tctctgcccg ccaggagcga ggcggaagag
     1741 cgaatcatcg cgaagatccg ggagcgctat ggccacgcgt ctgcggaacg cgtgctgtcg
     1801 gggccgggca tcatcgccct gcacagcgcc atgcatggcg agaacatgaa gacgtcccgc
     1861 gaaatcacga gcggcacggt cgatcgcaag tgccgcgcga cgatggagca gttctatcgc
     1921 ttcctcgcga ccgtagccgc cgatctcgcg atgaccaccg gggcatacgg cggtgtcttc
     1981 atcgcaggcg gcatcgttcc cgacaacatc gagcagatcc aggcatccac gttccgcgag
     2041 cgcttcgagg acaagaatcg ttaccggcac tacatgcagg ctatccccac ctacgtcgtt
     2101 accgacccca cgccagggct aaccggcctg atggcctaca tcgtcgagca tcaaagcggc
     2161 tgacctgttg atcggccacg cggccgactc cggaaattta ctcgtggtcg tcgactggcg
     2221 tggtatccga cctctccaga gccttgtcga tgtcttcctt gtgctgcatc gccgcagctt
     2281 cgacgtttct tgctttttcg agctgatcgg tcatcgggtc gaacacgccc tgttcctctg
     2341 gcgtgcccgt caccgcgggt tccctgctgt cgttgcaacc ggcaaggcac agcacgacgg
     2401 tcaccaggta cagtgtcttt atcattgctt cactccccct tcaaaaggaa ttcagtccgg
     2461 agccaaagcg gcggccagat ccacgacgaa ctgtcgcagg tagctgtcga tctcgttgat
     2521 gtttgccgaa aggcggtggt cgccattgac gatgtgcagc gtggccaggc atccgcgcgc
     2581 gtaacggatg ctgttctcga ctggcacgac atcgtcccgc catccgtgca cgatcgccag
     2641 ggggatatcc ggcgccggcg gagtaaggtg ctcgtaacct tgcatgtagt acgcgggcgc
     2701 gagaacgaat accccgatgg cgccgacggc ggaggccgct gcagtagcca cgtgaccacc
     2761 catgctcgag ccgaccagga tcaagggctc ctccacgctc gcgcaggtcc gcaccagttt
     2821 cgccacacgt tccgtcgggt cgtcgatccc ctggtagtcg acgctttcga cgcggcagcc
     2881 aagctccctg gccgtctccg ccatcgcctt gatcttcgta ccccagggcc cgctttcctg
     2941 gccgtgggaa aaaaagacgg caggcatggt catggcactc gctctccttc atcgtcccac
     3001 gcttcggtcg gcttccacag caattcgtcc aggtccggcg tccaccggta cttcttcatg
     3061 tcgacccttc
//
LOCUS       DASUOM010000389         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_177900,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000389 DASUOM010000000
VERSION     DASUOM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177900"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..543
                     /locus_tag="VFY27_08110"
     CDS             163..543
                     /locus_tag="VFY27_08110"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEX5765518.1"
                     /translation="MRQSVKAIAILLVTNLVTLGFVQSAGAGVISTEAAIEMHEREAR
                     IERISSVLARDSVQSRMIHLGVDPNVAAARIASLSDSELQQLEARMDELPAGADGALT
                     VLGIVLLVLLVLELVGVTDVFKSF"
     gene            636..1478
                     /locus_tag="VFY27_08115"
     CDS             636..1478
                     /locus_tag="VFY27_08115"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEX5765519.1"
                     /translation="MPTAVELVDTPFFPQQEFQCGPAALATVLVDSGVTIHPDELIGR
                     VYLPQRQGSLQPELLAAMRHYDRIPYVIPPEFPALLAEVSQGRPVLVLQNLGVKAFPA
                     WHYAVVIGYARERGEVVLRSGTDERRVTPADTFANTWRRGGNWGIVALQPGELPATDD
                     SDGYLRALAAAESAGRPDLAEPSYRAAAKRWPDSSLAWLGLGNSAYARGEQVAAERLY
                     RHALEIDSGNAAALNNLASTIAEQGRCDEAQQLLTTAMALPDLHVQMSDVLLQSRAEI
                     GTCK"
     gene            1504..1842
                     /locus_tag="VFY27_08120"
     CDS             1504..1842
                     /locus_tag="VFY27_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765520.1"
                     /translation="MMTRKWLTGLLVGVLLVPVLFEVQWNPQFVMPRTVARADEVQEA
                     RYRQCVTEQVDEATRQALAAADNPDVQSLMIRMRQKEVLADCRLQHPARRVDVDEPLR
                     VNLLDFRWRF"
     gene            1899..2642
                     /locus_tag="VFY27_08125"
     CDS             1899..2642
                     /locus_tag="VFY27_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765521.1"
                     /translation="MQLGTVTIARRFRGPPNSGNGGYSCGRLAAFVDGPAEVTLRRPP
                     PLELEMHVRSEEGGVAMYAGEQLIASARPVEPWRPDTAAPALEEALAATGRTFDPSVH
                     KLPMCYVCGPDRAPGDGLRIFCGPLDPTDTGWSGTVAAPWVPEPYMAGADRMVSDEYL
                     WAALDCPTAYASGSPAGFPTILLGRQAVSILNRPAVGEKCIIAARQTCRDGRKYLSEA
                     SLFNEAGTPLAHCRATWIEVPPDVQRGSV"
     gene            complement(3091..>4255)
                     /locus_tag="VFY27_08130"
     CDS             complement(3091..>4255)
                     /locus_tag="VFY27_08130"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5765522.1"
                     /translation="FDAHHAVRTRMRRLPLFASIIALTACGADPGSSAAAQDFPFEAT
                     PVATLDEPWAMTFLPDGRLLVTEKKGRLLVVTQEGDTSQPISGLPDVAYGGQGGLGDV
                     ALHPEFSDNGVIYLSYAEAGEGDVRGAAVMRATLTLTDDGGSLGDQQVIWRQEPKVTG
                     EGHYGHRIAFSGDGYVFITSGDRQKFTPAQDMQQNLGKIVRLNEDGTVPSDNPFASVG
                     GVSAQVWTLGHRNPLGITFDAEGRLWAQEMGPRHGDELNLVVRGKNYGYPVVSNGNHY
                     DGRDIPDHDTRPEFEAPKVFWVPAISPGGLMIYSGDLFPEWKGNAFLGGLSSEALIRV
                     TIDGDSAVEAERFAMGERIREVEQGPDGAIWLLEDEDDDGGEGGRLLRLTPSR"
BASE COUNT          787 a         1347 c         1314 g          807 t
ORIGIN      
        1 cggacgcaag cgtaccacgg aaacaagtcc ctgcattatg caatccggca gtgcagagcg
       61 gcagttgcac ggccttccaa tctggcattc gcctcattct tccccgataa ttcagccgtc
      121 ggctcacgtc gatccttcta aaattaccga gcgggtgatt acatgcgtca gtccgtaaaa
      181 gcgatcgcga tacttcttgt tactaacctc gtcactctcg gattcgtgca atcggccggt
      241 gccggcgtca tcagcaccga ggctgccatc gaaatgcatg agcgtgaggc gcgaatcgag
      301 cgcatcagtt ccgttcttgc aagagacagc gttcaaagcc gaatgatcca tctcggtgtc
      361 gatcccaatg tggccgcagc acggatcgcc agcctgagcg acagtgagct gcagcagctc
      421 gaggcacgca tggacgagtt gccagcggga gctgacgggg cgctgacggt tctagggatc
      481 gtgctcctcg ttctgctggt cctggaactg gtcggcgtta cggacgtgtt caaatcgttc
      541 tagtcgtgtt gctgcgatct gcggcattcc tggcgtttgt cggcgtaatc gctggttgtg
      601 ctgccgatcc gtttggggcg gcggtgcggc ctggcatgcc aaccgcggtc gaactcgtcg
      661 atacgccctt tttcccgcag caggaatttc agtgcggacc ggcggcgctg gcaaccgtcc
      721 tcgtagattc aggagtgaca atccatccgg acgagctgat cgggcgcgtt tatctcccac
      781 aacgccaggg cagcctgcag ccggagttgc tcgctgcaat gcggcactat gaccgcattc
      841 cttacgtcat cccgccggag ttcccggcat tgttggcgga agtgtcgcag ggacgccctg
      901 tcctggtact gcagaatctt ggcgtaaagg cgtttcccgc gtggcactac gccgtggtga
      961 tcggttacgc acgtgagcgg ggcgaggtcg tgctgcgctc tggaaccgat gagcgacggg
     1021 tgacgcccgc ggataccttc gcgaacacgt ggcgccgcgg cggcaactgg ggcatcgtcg
     1081 cgctgcagcc gggagaactt ccggcgaccg atgacagtga cggctacctg cgcgccctcg
     1141 ctgcggcgga atcggcggga cgtccggatc ttgcagaacc gtcgtaccgg gcggcggcga
     1201 agcgctggcc cgacagcagc ctggcctggc tcgggcttgg caattccgcg tatgctcgcg
     1261 gcgagcaggt tgccgcagaa cgcttgtacc ggcacgcact ggagatcgac tcggggaacg
     1321 ccgctgcctt gaataacctc gcgagtacga ttgcggaaca ggggcgttgt gatgaagctc
     1381 aacaattatt gacaacggcc atggcacttc cggacctgca tgtgcagatg tccgatgtac
     1441 tgctacagtc acgagccgag atcggcacct gcaaatgaag ggtgggtcgc gcggctgatt
     1501 gtcatgatga ccaggaagtg gctaaccggc ctgctcgtgg gtgtgctgct ggttccggtg
     1561 ctcttcgaag tgcagtggaa tccacagttt gtgatgccgc ggacggtggc gcgagcggac
     1621 gaagtgcagg aagcgcgcta tcggcagtgc gtcaccgaac aggtagacga ggcgacgagg
     1681 caggctctgg cagcggccga caacccggat gtgcagagcc ttatgattcg catgcggcag
     1741 aaggaggtgc tcgcggattg caggctgcag catcctgcac gccgggtcga tgttgatgag
     1801 ccgctgcgcg tcaacctgct ggattttcgg tggcgcttct gaaccacgag gctcgactga
     1861 tcgattctta caaccttacc ccggtccgag gataaggcat gcagctcggc acagtcacga
     1921 ttgcgcgtcg ctttcgcggc ccgcccaact ccgggaacgg cggctactcc tgcggtcgcc
     1981 ttgcggcttt tgtcgacggt cctgcagaag taaccctgcg caggccgcct ccgctcgagc
     2041 ttgagatgca cgtgaggtct gaggaaggag gggtagcgat gtacgcgggc gagcagctga
     2101 ttgcttccgc gcgccccgtc gagccgtggc gccccgatac agctgctccc gcgctcgaag
     2161 aagcacttgc cgcgaccggg cgaacgttcg acccctccgt acacaagctc ccaatgtgtt
     2221 atgtctgcgg ccccgaccgc gcgcccggcg acggactgcg gattttctgt ggccccctgg
     2281 atcccaccga caccggctgg tccggcaccg tcgctgcacc ctgggtaccg gagccgtaca
     2341 tggcgggcgc ggaccgcatg gtctcagacg aatacctgtg ggccgcgctc gactgcccca
     2401 ccgcgtacgc ctccggatct cccgcaggct tccccaccat actcctcggc cgccaggcgg
     2461 tctcgatcct caaccggcct gctgtgggcg aaaaatgcat cattgctgcc cgccaaacct
     2521 gccgcgacgg ccgcaaatac ctgtccgaag ccagcctgtt caacgaggcc ggcacccctc
     2581 tcgcccattg ccgcgccacc tggatagaag tcccacccga cgtgcagcgc ggctccgtat
     2641 agggcgttac cggattcggg acaccggatc acgggacacc caccaaattg ggaaccggtt
     2701 tatgggacat ccaccccttg gcggggacgg attttgggac acccatcatt tggcagaatc
     2761 acggtgaggg ggattacggg gaggggatca tggaacaacc aatctttatt ggaccgaggg
     2821 tcgaatcacg gcaccaggcg accattaggc aatacctatc aattaactcc ggagccgacc
     2881 ggggaaggta tgagctacct ccgtagcgga atgccaagag actaaccggc gtcctcaaat
     2941 acaggccaag tttcgtgggt gtccccgagt ggtgtccccg aatggccggt tcgtggctgt
     3001 cctcaccttc ctctcacctt cctcaaagcc gcgcatacgc cttccggacg ggcgggtggg
     3061 cgggagaaag gtggctgtcc cgatttgggg tcagcgggag ggggtgagtc tgagcaggcg
     3121 gccgccttcg ccgccgtcgt cgtcctcgtc ttcgagaagc cagatggcgc catcagggcc
     3181 ttgctcgacc tcgcggattc gctcgcccat cgcaaagcgt tcggcttcga cggccgaatc
     3241 gccgtcgatc gtcacgcgaa tcagcgcctc tgaagaaaga ccgccaagga atgcgttgcc
     3301 cttccactcg gggaacaggt cgcccgaata gatcatcagc ccgccgggag aaatcgccgg
     3361 cacccagaac acctttggtg cctcgaattc cgggcgcgta tcgtgatccg ggatgtcgcg
     3421 gccatcgtag tgattgccgt tcgaaaccac cgggtagccg tagttcttcc cgcgaacaac
     3481 gagattcagc tcatcgccgt gccggggtcc catttcctgg gcccacagcc gtccttctgc
     3541 atcgaaggtg ataccgaggg ggttgcgatg gcccagcgtc cagacctgcg cagaaactcc
     3601 gcccacgctc gcgaacggat tgtcagatgg caccgtaccg tcctcgttca ggcgcacgat
     3661 cttgcccagg ttctgctgca tgtcctgagc cggcgtaaat ttttgccggt caccgctagt
     3721 gatgaacacg taaccatcgc cggaaaaggc gatgcggtgc ccgtagtgac cctcccccgt
     3781 cactttcggc tcctggcgcc agatcacctg ctggtcgccc agcgatccgc cgtcatcggt
     3841 gagcgtcaag gtcgcacgca tcacagccgc cccgcggacg tcgccctcac cagcctccgc
     3901 atagctcagg taaatcacgc cgttgtcgct gaactccggg tgtagcgcca cgtcgcccaa
     3961 tcccccctgc ccgccatagg ccacgtcggg cagtccggag attggctgcg acgtatcgcc
     4021 ttcctgcgtc accaccagca gccgtccctt cttctcggta accagtaacc ggccatccgg
     4081 cagaaacgtc atcgcccaag gctcatccaa agtcgcaaca ggagtcgcct cgaacggaaa
     4141 gtcctgtgcc gcggcactgg aaccgggatc ggctccacag gcagtcaagg cgataatgct
     4201 ggcgaaaagg ggcaggcgtc tcatacgtgt cctcactgcg tggtgggcat caaag
//
LOCUS       DASUOM010000390         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1105102,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000390 DASUOM010000000
VERSION     DASUOM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105102"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..804)
                     /locus_tag="VFY27_08135"
                     /pseudo
     CDS             complement(1..804)
                     /locus_tag="VFY27_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611480.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(870..1292)
                     /locus_tag="VFY27_08140"
     CDS             complement(870..1292)
                     /locus_tag="VFY27_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765523.1"
                     /translation="MNIDIPLRELGPVDSTALRDAVLAQVDRAWQEDIYRQEAFEVQR
                     SIQSIVVLFVDIDRWPDIVVEKEPGWPRLADAALPVMNDITTSPAARSYAMAAKLVAG
                     GKITPHVDRHPSFHHGHRIHVPISTSSRVRFMIDGKPY"
     gene            1443..1793
                     /locus_tag="VFY27_08145"
     CDS             1443..1793
                     /locus_tag="VFY27_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765524.1"
                     /translation="MIDDNDLGDTAELDLIRTAEFEHLDAEVRGGADDLQNGSSGKPG
                     LSSVQRDCMNRAELVLALNEWMRKFIEAPREFDEQMHRVAQALKDNGEAPSYGLAAVG
                     FLEMCLDDAKKRMT"
     gene            1810..>2578
                     /gene="mmuM"
                     /locus_tag="VFY27_08150"
     CDS             1810..>2578
                     /gene="mmuM"
                     /locus_tag="VFY27_08150"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEX5765525.1"
                     /translation="MNPPQPNLFENALSRDLPIALDGGLATELEARGCDIGTSLWSAA
                     LLRSNPGAIVDAHRAYLEAGAEIIISASYQASVRGFTSTGLSVQEAEALIASSVELAC
                     IARQQFCDAHPEINHTPIVAASIGPYGAALHDGSEYTGDYDVDESGLRRFHEQRLSLL
                     DQTNADVLACETIPNSLEAGVLCELLENVRLPAWVSFCCRDERLISDKTPLREVCAPF
                     QGHPRVLALGVNCTAPGFVASLVGEIGKAAPDKAVIVY"
BASE COUNT          522 a          783 c          743 g          530 t
ORIGIN      
        1 tgatcggtgg cacggcctgc ggatagccac ggaactgcgc cacacggtcg agtagtttca
       61 gtcacatcat cgccggtgac gctgtgcccc acctcgatca ttacggcttc cttggtaaag
      121 tcatcgacga ccgtcaggat cttcagtcgt cgactgctgc cagagcgtca tgatgaagtc
      181 catggaccac acctggttgg tgcgactcgg caacttcaac ggctgccggg ccacggcaat
      241 gccgcgaagc cgctggcggc gccgaacgct caagtcagct tcccggtaca gccgatagat
      301 tttcttgtgg ttcacaacat gtccttcccg acgcagcagc acatgcagcc gccgataacc
      361 gaaccgcttg cgctcgcctg cgagttcccg taatcgatcc tgaagcgcgc tgtcgctctc
      421 ccgcgtctcc gactgatagc tcaagaccga acgcgataac cccaccagac tgcaggccct
      481 gcgttgcgag atcccggtcc tggcctgcat cgtcgcaatc gcgcagcgct tgtcgtccgc
      541 ggtcagtact tttttgccag cgccgccctc aacgcgtcgt tgtccagtat cgagtccgcc
      601 agcagccgct tgagcttccg gttctcctcc tcgagctgct tgagtcgctt gagatcctcg
      661 gtgtccatct cgatacttct tacgccaggt gtagaaagtc gcatccgaga tgttgtgctt
      721 ccggcagatc tccttcaccg gcactccggc ttccccctgc ttcagaatcg caacgatctg
      781 ctgctcagaa aatcgcttct tcatgtccgt cctctcctcg gaaaacggac tctgctaagc
      841 tcaaactggc ccggctacag gggggcaggt cagtagggtt tgccgtcgat catgaagcga
      901 acgcgggagc tggtcgaaat cggcacatga atgcgatgcc cgtgatgaaa tgacggatgc
      961 cggtcgacgt gcggcgtgat cttcccgccg gcgaccagct tcgccgccat cgcgtatgac
     1021 cgtgccgccg ggctcgtagt gatgtcgttc atcaccggca acgcggcatc cgcgagccgc
     1081 ggccagcccg gctccttctc gacgacaatg tccggccatc ggtcgatgtc gacaaacaac
     1141 acgacgatcg actggatcga gcgctgaact tcgaaggctt cctgccgata gatatcctcc
     1201 tgccatgccc ggtctacctg ggcaagcacc gcatcacgaa gggcggtgct gtcgacgggg
     1261 ccgagttcgc ggagcggaat gtcgatgttc ataggctgga cgtaaaccta gccaagtggg
     1321 aatgatacag ggtatgacgt caccgccgca tgctgtcgga gcctgaggaa cctgaacctg
     1381 catcacggac tcacgattgt cccggcttta ccgtgccagt tcgtaaccgt gagataatcc
     1441 ttatgattga cgacaacgac ctgggtgaca cggccgaact cgacctgata cgcaccgctg
     1501 aattcgaaca ccttgatgct gaggtccgcg gcggggccga tgaccttcag aacggtagta
     1561 gcgggaagcc cggcctctca tccgtgcagc gggattgcat gaacagagcg gaactggttc
     1621 ttgcgctgaa cgagtggatg cggaagttca tcgaagcacc acgagaattc gatgagcaaa
     1681 tgcatcgcgt agcacaggct ttgaaagaca acggagaggc ccccagctat ggcttggctg
     1741 ccgtcggatt cctggagatg tgcctcgatg atgcaaagaa acgcatgact tgaaatcttg
     1801 ccaggtgaca tgaacccgcc acaaccgaac ctgtttgaaa atgcgctgtc cagggatctc
     1861 ccgattgcgc ttgacggcgg ccttgcaacc gaactcgaag cacggggatg tgacatcggt
     1921 accagcttgt ggtcggcagc gcttctccgg tcgaaccccg gggccattgt cgatgcgcac
     1981 cgcgcctacc tcgaagccgg cgcggaaatc atcatcagtg cgagttacca ggctagcgtc
     2041 aggggcttta cgtcgacggg cctgtcggtt caggaagctg aggcactcat tgccagttcg
     2101 gtcgagttgg cttgcatcgc cagacaacag ttctgcgacg cgcatccaga gataaatcac
     2161 acccctatag tggcggcaag cattgggccc tacggcgcag cccttcatga cggctcagaa
     2221 tacaccggtg actacgacgt cgatgaatct ggcctgcgtc ggtttcatga gcagcgcctt
     2281 tcgctgcttg atcagacaaa tgccgatgtc ctggcgtgcg agacgatccc gaacagtctc
     2341 gaagccggcg tactatgcga gctgctggag aacgttcgcc tgccggcctg ggtgagtttt
     2401 tgctgtcgtg acgagcgcct gatcagcgac aagacgccgc tacgggaagt ctgtgccccg
     2461 tttcagggac atcctagggt acttgcactc ggcgtcaatt gcacagcacc cggctttgta
     2521 gcgtcattgg tcggggagat cggaaaggca gcacctgaca aggctgtcat tgtctacc
//
LOCUS       DASUOM010000391         8505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_143610,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000391 DASUOM010000000
VERSION     DASUOM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143610"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..559)
                     /locus_tag="VFY27_08155"
     CDS             complement(257..559)
                     /locus_tag="VFY27_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5765526.1"
                     /translation="MTKSELIEMIARKQKHLPAKDVELAVKHLLDLMSDALARGERIE
                     IRGFGSFSLHFRPPRIGRNPKTGEAVALSGKYVPHFKPGKDLRERVNASREFPIKN"
     gene            710..1012
                     /locus_tag="VFY27_08160"
     CDS             710..1012
                     /locus_tag="VFY27_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5765527.1"
                     /translation="MTKSELIEMIARKQKHLPAKDVELAVKHLLDLMSDALARGERIE
                     IRGFGSFSLHFRPPRIGRNPKTGEAVALSGKYVPHFKPGKDLRERVNASREFPIKN"
     gene            1193..1489
                     /locus_tag="VFY27_08165"
     CDS             1193..1489
                     /locus_tag="VFY27_08165"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX5765528.1"
                     /translation="MLKRIGAVLLVLLILGLMLVFTRLNPGTIDIDLAVAKVESSIPL
                     AFTIAFAAGWLFGLLCMSFVAVKLINERRRLRRALRNTEVEVSSLRNLPLSDAD"
     gene            1479..2690
                     /gene="lapB"
                     /locus_tag="VFY27_08170"
     CDS             1479..2690
                     /gene="lapB"
                     /locus_tag="VFY27_08170"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX5765529.1"
                     /translation="MPTESTFLLAGLFLLLVAAGWALGRFGEREEREGAPPPLNVDYL
                     KGLTFLLNEQTDQALEHFLKMVRVDDKTIETHFALGSLFRRRGEVDRAIRIHQNIIAR
                     PDLASEQRDQALFSLAKDYLRAGLLDRAEKLFVRLSQGSRYQVEALEQLCRIYEQERE
                     WQHAINAGQKLEVLSGKSLALQIAHYYCELAEQAAAKKDFAAARAFVKKAQSGRPRTL
                     RGALTRAEIAQATDDTKTALRLYHRIIDESTYLITEALPKLVEIHAREGSIEELERDL
                     RTLLDKNPQMTTDVAYTAIVNNIGGVPVIDECVENYMLNEPTLTEFIDLQRMNQVGKS
                     ERDAALAKVRSALSRLAASTPRYKCTECGFSSQRLLWQCPSCKNWETQRPFTRVQFDS
                     LLQRSAAIRQE"
     gene            2847..3125
                     /locus_tag="VFY27_08175"
     CDS             2847..3125
                     /locus_tag="VFY27_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5765530.1"
                     /translation="MKNIKYCLSLFVFLLAGSALAGPVDVNTADAATLSAELQGIGMS
                     KAVAIVEYREANGPFKSPDDLILVKGIGERTIELNRDNILVQPQDSPE"
     gene            3275..4147
                     /gene="galU"
                     /locus_tag="VFY27_08180"
     CDS             3275..4147
                     /gene="galU"
                     /locus_tag="VFY27_08180"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5765531.1"
                     /translation="MAKPIRSAVFPVAGRGTRFLPATKASPKEMLPVVDKPLIQYAVE
                     EAIAAGATKLVFVTGSSKRAIEDHFDSDPELEKALSGAGKEDLLQSIQGIVPRGVSCI
                     YIRQGEPLGLGHAVLCARPAIGDEPFFVHLADDLIAGEPSCLSQMAAEYQEHGGSVIA
                     VETVPEEDTSSYGIVAVEKEPGGSQRIRKIVEKPKPADAPSNLAVVGRYLLSADVFDK
                     LERTTRGAGGEIQLTDAIADMLEESPVYAYAFSGERFDCGNKLGYLKASVAYGLAHPD
                     IGEAFRAHLRGVLD"
     gene            complement(4167..6194)
                     /locus_tag="VFY27_08185"
     CDS             complement(4167..6194)
                     /locus_tag="VFY27_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5765532.1"
                     /translation="MAEAEDLITDAARHATVFMQDLWRRHAVAPDAAQPLSFSDIARR
                     LDLLVAAAFGTSYPLRIAQNPAPATLLVKLFERHDRPRVAHAVPATDGAHIWLPARIA
                     AETDAVILLRFRTMALQQAARAARGSAAAGSALPDALARDLYLLLEADAADRELAHLL
                     PGMRGPLNELRRCALSRRPELTEFPTVRRPVEKLVRQVLGTDCASHCPALPVTESPAQ
                     SAILAPKLAAKIAPEGVPGSRPMYKDLWSGDLRPATPARASAAASTGFEQDDEGDVTT
                     PRGARLDRRPDVREAAEDEDKQEPGAWMIQSAQPEQHIEDPVGAQRPTDRDDQTAAED
                     FADSMSELPEARLVSTPGRPKEVLLSDDPPDARTTSEGSVEEEGGSRTSYPEWDFRIN
                     AYKDAAATVRSIRAPCGQQLWVDKTLDEHHAMLSAIRRRFEMLRSQRVRLRKQLEGDE
                     LDLGACIDNYADFRAGLPMADGLYETRRAARRSMAISLLIDTSGSTDGWVSASRRVID
                     VEREALLLVCIALEGMAEPYSVHAFSGEGPHGVTVREIKRFNEHYDNDVALRIAALEP
                     ERYTRAGAALRHVTTMLMQQGAEHRLLLLLSDGKPNDVDHYDGRYGVEDMRQAVNETK
                     LQGISPFCLTIDRQAANYLPAMFGAQHYAMLPRPELLPTVLVDWLRRLVVG"
     gene            complement(6226..7047)
                     /locus_tag="VFY27_08190"
     CDS             complement(6226..7047)
                     /locus_tag="VFY27_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX5765533.1"
                     /translation="MTGGVSTKPAVAVPGHAEPYYVPLGNEIPIFERCHAGCLAVMLK
                     GPTGCGKTRFVEHMAWRLRRPLVTISCHDDLTASDLIGRFLIRHDGTVWQDGPLTRAV
                     REGAICYLDEVVEARQDTVVVLHALTDYRRILPIDKTGEVVEAAPGFQLVISYNPGYQ
                     RMLKDLKPSTRQRFVALDFDFPPLDLEASIVAHEGGTDHATAHALVSLGHSLRKLKDR
                     GLDEVPSTRLLIAAGRLIASGIGVREACYAALVSPLSDDASLVAAMSDLVDATFV"
     gene            complement(7047..8426)
                     /locus_tag="VFY27_08195"
     CDS             complement(7047..8426)
                     /locus_tag="VFY27_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5765534.1"
                     /translation="MQYRSQSVAYWYFAVAMVLFGLQLVFGLLSAAKYLGPDPLLYIL
                     PFDVTKVIHTNLLIVWVLTGFMGATYWMVTSESRTEIYSVKLAYIQLILWVVMGVTAV
                     IGYLFRYGTGNKLLEQPLPHKITIVIVMLIFLYNVGRTIKQSGRFTTTEGVLLLGLAS
                     AAVLYLPALLHYENYTVSIFYRWWTIHLWVEGVWEMIQGGFLAYLLIRLSGADREVMD
                     KWLYVIVGLVFFAGILGTAHHYYWVGVPHYWLPIGGFFSALEPLALVGMAMYAYYAMR
                     RSGFSHPNTLALHWTIGSALFTMFGAGLLGLAHTWPSVNKWTHGTLITAMHGHAAFYG
                     AYAMITMAMITYALPSLTRGRKEGGTMLGYAAFWLQLAGMFGMTLSFATAGIAQVYLE
                     RILGLGYLDTQLKIQVHFLMLIITATIFTVGVIFFIVDFFRHPPRFETVDEERPGLRQ
                     PGETLPQST"
BASE COUNT         1716 a         2549 c         2628 g         1612 t
ORIGIN      
        1 accgaacaac cagcccgcca cgaacgcgat ggtgaatgcg agcggaatgg aagattcgac
       61 tttggcgacg gcgaggtcaa tgtcgatggt gcctgggttc agccgggtga ataccagcat
      121 caggccgagg atgagcagca ccagaagaac cgccccgatt cgtttgaaca ttcgcccgct
      181 ccgtttatgg gcacccatcc tgggcacaag cgccatcgaa tgggaagaat agcaacgctg
      241 gcaccccgaa acggttttag ttcttgatcg ggaactcccg gctggcgttg actctttcgc
      301 gcaggtcttt accgggcttg aaatgcggga cgtacttgcc ggataaggca acggcctctc
      361 ctgtcttcgg attccggccg atgcgcgggg gacggaaatg cagcgagaag ctgccgaaac
      421 cgcgaatttc gatccgttct ccccgcgcga gcgcgtcgct catcaaatcc agcagatgct
      481 tgaccgccag ttctacgtct tttgccggga ggtgcttctg cttccttgca atcatttcga
      541 tgagttcaga ttttgtcata acacgcttca ctttttatat ctgatacctg tccctgtaac
      601 ccggcgatta caaaagaaat atagcccgga ccaggtggtc cgggctatat tccttttgta
      661 atcgccgggt tacacggaca ggtatcagat acaaaaagcg aagcgtgtta tgacaaaatc
      721 tgaactcatc gaaatgatcg caaggaagca gaagcatctc ccggcaaaag acgtagaact
      781 ggcggtcaag catctgctgg acctgatgag tgacgcgctc gcgcggggag agcgaattga
      841 aatccggggg ttcggcagct tctccctgca cttccgtccg ccgcgcatcg gccggaatcc
      901 taaaacgggg gaggccgttg ccttatccgg caagtacgtc ccgcatttca agccgggcaa
      961 agacctgcgc gaacgagtca acgccagccg cgaatttccg ataaagaact gaagcggcgg
     1021 tgcgtcgccg gagaggggcc ttcacagccc gttgcacctg gcccttcagt gagtcaccgc
     1081 gatgatcgca gccaggcaaa gggcgcgggc gtatccaccg gggtaacata gtcttccaac
     1141 cagggcgcag cgggcgatcg ctgccggttt ccggttgtaa gggagcgggc gaatgttaaa
     1201 acgaatcggg gcggttcttc tggtgctgct catcctcggc ctgatgctgg tattcacccg
     1261 gctgaaccca ggcacaatcg atatcgacct cgccgtcgcc aaagtcgagt catctatccc
     1321 gctggcgttc accatcgcgt tcgcggcagg gtggttattc ggcctgctct gcatgtcctt
     1381 cgtcgccgtc aagctgatca acgaaaggcg gcgcctgcgc agggcattac gcaacaccga
     1441 ggttgaggtc agcagcctgc gcaacctgcc cttgtccgat gccgactgag tcgacatttc
     1501 tccttgcagg acttttcctg ctgctggtcg ctgccggctg ggcgctgggc cgcttcggcg
     1561 agcgtgaaga gcgagagggg gcgcctccgc cgttgaacgt cgactacctc aaggggctca
     1621 cgtttctgct gaacgagcag acggaccagg ccctggagca tttcctcaag atggttcgtg
     1681 tcgacgacaa gaccatcgag acgcacttcg cgctcggtag cctgttccgc cgccgtggcg
     1741 aggtcgatcg ggccatccgc atccaccaga acatcatcgc ccggccggac cttgcttcgg
     1801 aacagcgaga ccaggcgctg ttttctctcg caaaggacta cttgcgcgca gggctgctgg
     1861 atcgcgccga gaagctgttc gtccgtctct cccagggctc ccgctaccag gtcgaagcac
     1921 tcgagcagct ttgccgtatc tacgagcagg agcgcgaatg gcagcatgcc atcaatgcag
     1981 gacagaagct ggaagtcctg agcgggaaat cgctcgcact gcaaatcgcg cattactact
     2041 gcgagttggc cgagcaggcg gccgcgaaga aggattttgc cgctgcccgg gcgttcgtca
     2101 agaaagcgca gagcggacgt cccagaacgc tcagaggcgc cctgacgcgc gccgaaatag
     2161 cccaggcgac cgacgacacg aagaccgcgt tacgcctcta ccatcgaatc attgacgaga
     2221 gcacctacct gatcaccgag gcgctgccga aactcgtgga gatccacgca cgggaaggtt
     2281 ccatagaaga actggagcgg gacttgcgta ccctgctcga caagaatccg cagatgacga
     2341 cggacgtcgc ctacaccgca atcgtcaaca acatcggcgg agtgccggtg atcgacgagt
     2401 gcgtcgagaa ctacatgctc aatgaaccga cgttgaccga gttcatcgat ctgcaacgga
     2461 tgaaccaagt cggcaagagc gagcgtgacg ctgcgctcgc caaggtccgc tcagcgctga
     2521 gccggctggc ggcgtccacg ccgcgctaca agtgcaccga gtgcgggttc tccagccaac
     2581 gcctgctatg gcaatgcccg agctgcaaga actgggaaac ccagcgtccg ttcacacgcg
     2641 tccagtttga ctcgctgttg caacgcagcg ccgccatccg gcaggaatag cgcaggagcg
     2701 catttccgct caaacgcctg aaattcaccg gcggcaatgt tcccgcggcg gacctactct
     2761 ccccatcgaa ccacgtcgct ggttctggac tcgggtcgag tccaggaggc gacgacaggg
     2821 aagattcaaa tcgtcaggag cagagtatga aaaacatcaa gtattgcttg tcgctgttcg
     2881 ttttcctgct ggccggttcg gcgctggccg gtcccgtcga cgtcaacacg gcagacgccg
     2941 ccacgctgtc tgcggagctg cagggaatcg gaatgtcgaa ggcagtcgcg atagtcgaat
     3001 accgtgaggc aaacggaccg ttcaagagcc cggacgacct gatcctcgta aaggggattg
     3061 gcgagcgcac gatagagctg aaccgcgaca acatcctggt gcagccgcag gattctccgg
     3121 aataaatgca gcagtcgttg tagtttttac cgcctgtgcc ggttctgccg gctcaggcga
     3181 ttattttttt gcgcgcagag gcctcgcacc cgcactcccg cgtttgggcc agattgttta
     3241 tcatccctcc agttcccaaa cgttggagat tgtgttggcc aaacctatcc gatctgccgt
     3301 gtttcctgtc gccggacggg gtacgcgttt tctgccagcc accaaggcca gtccgaagga
     3361 aatgctgccg gtcgtggaca agccgctgat ccagtatgcg gtcgaggagg cgatcgccgc
     3421 gggtgccacc aagttggttt tcgtcaccgg gagttccaaa cgcgccatcg aggatcattt
     3481 cgactccgac cctgaactcg agaaggctct ttcgggagcc gggaaggagg acctcctgca
     3541 gtccatccag ggtatcgtgc cccggggggt gtcctgtatc tatatccgcc agggcgagcc
     3601 gctgggcctg ggtcatgcgg ttctctgtgc gcgtccggca ataggcgacg aaccattctt
     3661 cgtgcacctc gcggacgacc tgatcgccgg cgagccttcc tgcctgtccc agatggctgc
     3721 ggaataccag gaacacggtg gcagtgtcat cgcagtcgag acggtgcccg aggaagatac
     3781 ctccagctac ggtatcgtag ctgtcgaaaa agagcctgga ggctcccaga gaatccgcaa
     3841 gatcgtcgaa aagccgaaac ccgcggatgc gccgagcaac ctcgccgtgg tgggccgcta
     3901 ccttctctct gccgatgtgt tcgacaaact cgagcgaacg acgcgcggtg caggcggaga
     3961 gatccagctg acggacgcca tcgccgacat gctggaggag tcgccggttt acgcctacgc
     4021 gttcagcgga gagcgcttcg actgcgggaa caagcttggg tacctgaaag cgtcggtagc
     4081 gtacggactc gcgcacccgg atatcggtga ggcgtttcgg gcgcacctgc gcggcgtgct
     4141 ggattgacga ttttcgaaac cactcgctaa cccaccacca ggcggcgcag ccagtcaacc
     4201 agcaccgttg gcaggagttc cgggcgaggc agcatcgcat agtgctgtgc gccgaacatt
     4261 gccggcaggt aattcgcggc ttgccggtcg atggttaggc aaaagggact gatgccctgc
     4321 agcttcgttt cgttcacggc ctggcgcata tcctcgacgc cgtagcggcc gtcgtaatgg
     4381 tcgacgtcgt ttggtttgcc gtccgacagc agcagcagta gcctgtgctc tgcgccctgc
     4441 tgcatgagca tcgtggtcac gtgccgcaag gccgcgccgg cgcgggtata gcgctcgggt
     4501 tcgagcgccg cgatgcgaag ggcaacatcg ttgtcgtaat gttcgttaaa gcgcttgatt
     4561 tcgcgcaccg ttactccgtg cggaccctcg ccggaaaaag cgtgcaccga gtacggttcg
     4621 gccatgcctt cgagagcgat gcacaccagc aggagcgctt cgcgttctac atcgatcacc
     4681 cgcctgctgg cggagaccca gccgtcggtt gagccactgg tgtcaatgag cagactgatc
     4741 gccatgctcc gccgcgccgc gcgacgggtc tcgtaaagac cgtcggccat cggtaagccg
     4801 gcgcggaagt ccgcatagtt gtcgatgcag gcgccgagat ccagttcgtc gccctcgagc
     4861 tgcttgcgca accggacgcg ctgcgatcgc agcatttcga agcgccggcg gattgcggag
     4921 agcatcgcgt gatgctcgtc gagggtcttg tcgacccaca attgctgccc gcagggcgcc
     4981 cggatgcttc gcacagtcgc tgctgcatcc ttgtaggcat tgatgcggaa gtcccattcc
     5041 ggatagctgg tacgtgagcc tccttcctcc tcgacactgc cttcgctcgt tgtcctcgca
     5101 tccggcgggt catccgaaag aaggacttct ttcgggcggc ctggtgttga gaccagccgt
     5161 gcctcgggaa gctcggacat ggagtcggca aaatcctcgg cggcagtctg gtcatcgcga
     5221 tcggtcgggc gctgtgctcc cacaggatct tcgatgtgct gctcgggctg cgcggactga
     5281 atcatccagg cacctggctc ctgcttgtcc tcgtcttccg cggcttcacg cacatcgggc
     5341 cggcgatcca gtcgagcgcc gcgcggcgtc gtgacgtcgc cttcgtcgtc ctgctcaaac
     5401 cccgtactgg ctgcagccga tgccctggcg ggggtcgcgg gacgcagatc gccgctccac
     5461 aggtccttgt acatcggtcg ggatccggga actccttccg gcgcgatttt tgccgcgagt
     5521 ttaggagcca ggattgcgga ttgcgcagga gattccgtaa cgggcaacgc ggggcagtga
     5581 ctggcgcagt ccgtgccgag aacttgtctt acgagttttt caacggggcg gcggacagtg
     5641 gggaattcgg tcagctccgg acgtcgcgac aaggcgcacc ggcgcagctc attcagcggc
     5701 ccgcgcattc cgggaagaag gtgagcgagc tcccggtctg cggcatccgc ttccagcaac
     5761 aggtacaggt cccgtgcgag cgcatccggc aaagcggagc cggccgcagc actgcctcgc
     5821 gcagcgcggg cagcctgctg caacgccatg gtgcgaaacc gcaggagaat aacggcatcg
     5881 gtctctgcag cgatgcgggc aggcagccag atgtgcgctc catccgtcgc gggaaccgca
     5941 tgggcgacgc gcggccggtc gtgacgctcg aacagcttca cgagcaaggt ggcaggcgcc
     6001 ggattttgcg cgatgcgcag cggatagctg gtgccgaacg cggctgcaac caacaagtcg
     6061 aggcgccgcg ctatgtcgga aaacgaaagc ggttgtgcag catcaggggc tactgcatgc
     6121 cggcgccaga ggtcctgcat gaagaccgtt gcgtggcgag ccgcatctgt aatcagatct
     6181 tctgcttcag ccatggctca cacgcgcaga ttaccggccg gttcgttaga caaaggtcgc
     6241 gtcaaccaga tcgctcatcg ctgcaacaag cgaagcgtcg tccgacagcg gtgacacgag
     6301 ggccgcatag caggcttcac ggacgccgat gccgctggcg atcagcctgc ccgcggcaat
     6361 gagcaagcgc gtgctgggga cttcgtccag tccacgatcc ttcagcttgc gcaggctgtg
     6421 cccgagcgag acaagtgcat gcgccgtcgc atgatccgtc ccgccttcgt gggcgacgat
     6481 ggacgcttcc aggtcgagag gcggaaaatc gaagtccaag gcgacgaaac gctgacgcgt
     6541 gctgggtttc aggtccttca acatgcgctg gtagccggga ttgtatgaga ttacgagctg
     6601 gaatccgggc gctgcctcaa cgacttctcc ggttttgtcg atcggcagga tgcgccggta
     6661 gtccgtcagt gcgtgcagca ccaccaccgt atcctggcgc gcctcgacga cctcgtcgag
     6721 gtagcagatc gcgccttcac gaactgcccg ggtcaaggga ccgtcctgcc acaccgtgcc
     6781 gtcgtgccgg atcaggaaac ggccgatcag gtcgctcgcg gtcaaatcat cgtgacagga
     6841 aatcgtgacg agcggacggc ggagccgcca tgccatgtgc tcgacaaagc gcgtcttccc
     6901 gcatccggtg ggccccttga gcataaccgc aaggcatccg gcgtggcagc gttcaaaaat
     6961 cgggatttcg ttccccagcg gaacgtagta aggctcggcg tgcccgggta ccgcgacggc
     7021 aggtttcgtg gagacgccac cggtcatcac gtgctctgcg gtagcgtctc gccgggctgt
     7081 ctcagccccg gcctctcttc atcgactgtt tcgaaacgtg gagggtggcg gaagaaatcc
     7141 acgatgaaga agatcacgcc cacggtgaaa atcgtcgccg tgatgatcag catgaggaag
     7201 tgcacctgga ttttgagctg ggtgtcgaga taacccaggc caagaatacg ttccaggtaa
     7261 acctgggcga taccggccgt agcgaacgac agggtcatgc cgaacatgcc ggcaagctgc
     7321 agccagaagg ccgcgtatcc cagcatcgtc ccgccttctt tccggcctcg ggtcaggctc
     7381 ggcaatgcgt aggtgatcat cgccatggtg atcattgcgt aggctccgta gaaagccgcg
     7441 tggccatgca tggcagtgat caatgtgcca tgggtccatt tattgacgct cggccaggtg
     7501 tgggcgagtc ccaggaggcc ggccccaaac atggtgaaca acgcgctgcc gatcgtccag
     7561 tgcagcgcca gcgtgttggg atgggagaac ccggatcgtc gcatcgcgta gtaggcgtac
     7621 attgccatcc cgaccagcgc gagaggctcg agcgcgctga agaacccgcc gatcggcaac
     7681 cagtagtgcg gcacgccgac ccagtaataa tggtgcgccg taccgagaat ccccgcgaag
     7741 aacaccagcc cgacgatgac gtaaagccat ttgtccatca cttcccgatc tgcgccagaa
     7801 aggcggatca ggagatacgc gaggaacccg ccttgtatca tctcccacac gccttccacc
     7861 cacaggtgaa tcgtccacca gcggtagaaa atagataccg tgtagttttc gtagtgcagc
     7921 agcgcaggca gataaagaac ggcggcgctt gcgagtccga gtaagaggac gccttccgtg
     7981 gtggtaaagc ggccggattg tttgatggtc ctgccgacgt tgtagagaaa tatcagcatc
     8041 acgatgacga tggtgatctt gtggggcagg ggctgctcca gcagtttgtt gccggtgcca
     8101 tagcgaaaca ggtagccgat gaccgccgtc acacccatga ccacccacag gatcagctgt
     8161 atgtaggcga gcttgacgct gtagatctcg gttctcgatt ctgacgtgac catccagtag
     8221 gttgcaccca tgaatcccgt cagcacccag acgatcagca ggttcgtgtg gatcaccttc
     8281 gtaacgtcga aaggcaggat gtaaagcagc ggatccgggc cgagatactt ggcggcagaa
     8341 aggaggccaa acaccagttg cagcccgaag agcaccattg caacggcgaa ataccagtag
     8401 gcaacggact gtgatctata ttgcattgcg gctctccctc tcgtttttct ttggtgctgc
     8461 tttcatcggc tctcggcggg tattagcgaa ggctggcgag gtagg
//
LOCUS       DASUOM010000392         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1345578,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000392 DASUOM010000000
VERSION     DASUOM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345578"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1414
                     /gene="dnaA"
                     /locus_tag="VFY27_08200"
     CDS             86..1414
                     /gene="dnaA"
                     /locus_tag="VFY27_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5765535.1"
                     /translation="MQTLWDACLRRLEQELPAQQFNTWIRPLAADATQAVPDTLVLCA
                     PNRFVLELVRERFAARIERLAAESCGRELGLRLVLAHTEPAAQRAPSNRTAGAAPPAL
                     ERARLNRLFTFDSFVTGKANQLARAAAIQVAENPGISYNPLFIYGGVGLGKTHLLQAI
                     GNHLAEQRPQARLRYTHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSNK
                     GRTQEEFFYAFNALVEAHKQIVISCDTYPKEIAGMEERLISRFGWGLTVAIEPPELEM
                     RVAIVLKKAEAEAAQISEEIAFFIAKNIRSNVRELEGALKKVLAFARFTGREITLEMA
                     KEALRDILNVASRQISVDGIQKTVAEYFKLKISDMHSKKRSRNVARPRQVAMSLAKDL
                     TPMSLPEIGEAFGNRDHTTVLHACRTISMLRKKDSVLNRDYLVLEQVLRG"
     gene            1625..2731
                     /gene="dnaN"
                     /locus_tag="VFY27_08205"
     CDS             1625..2731
                     /gene="dnaN"
                     /locus_tag="VFY27_08205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5765536.1"
                     /translation="MALVKAKRDELLGPLSAVSGIIERRHTLPILSNVLIDRVDDTLS
                     FLATDIEIQITARSTLPAGADAKPVTVGARKLVDILRALPDSTEVTVQMQDKRLQVKS
                     GKSRFTLQTLPAEDFPRLARPSGEMASFALPQKALRRLLGLVQYAMAQQDIRYYLNGL
                     LVLVEDQQLRLVATDGHRLAYASMKLPASLPRLEVIVPRKTVLELSKLLGDGEEEVKI
                     DLSGAQAAFNLGTVELVTKLVDGKFPDYTRVIPTQHKNLVKLEREVLRQALQRAAILS
                     NEKFRGVRWVLTDGSLKIVSSNTEQEEAQEELEVEYSGDAIDIGFNVNYLLDVLNNVS
                     GNEVECHFGDAASSALLQFPAEPDFKYVVMPMRI"
     gene            2743..>3592
                     /locus_tag="VFY27_08210"
     CDS             2743..>3592
                     /locus_tag="VFY27_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5765537.1"
                     /translation="MGESEQQRPDGGDYDSDSIKVLKGLEAVRKRPGMYIGDTSDGTG
                     LHHMVFEVVDNAVDEALAGYCDEIIVTIHTDNSISVLDNGRGIPTGIKEDDELKRSAA
                     EIVMTELHAGGKFDSNTYKVSGGLHGVGVSVVNALSSWLRLTIWRDARVHQLEFADGA
                     PTAPLRVTGTSEKRGTEVHFLASRQIFGNVDYHYDILARRLRELSFLNNGVRILLVDQ
                     RSGKEEDFAFTGGVKGFVEYMNRAKTVLHPNVFSAQGEKDGLSVDVAMQWNDSYQENV
                     ICFTNNI"
BASE COUNT          774 a         1079 c         1090 g          649 t
ORIGIN      
        1 agagtaagat ttcgacctcg ccggcgcgcc aggcgcagcc ccggtcaagc cctcgaacaa
       61 cgcgctgcac gagatcgccg aaaacatgca aaccctatgg gacgcctgcc tgcgccgcct
      121 tgagcaggag cttcccgcgc aacaattcaa cacctggatt cgtccgctcg ccgccgacgc
      181 gacgcaggca gtgccggaca cgctggtgct ttgcgcgccg aatcgcttcg tgctggagct
      241 ggtacgcgag cgctttgccg cccgtatcga gcgtcttgcg gcggaatcct gtggtcgcga
      301 actcgggctg cgcctagtac tggcgcacac cgaaccggcg gcacagcgcg cgccctccaa
      361 tcgcactgcc ggtgcagcgc caccggccct ggagcgggca cgtctcaatc gtctgttcac
      421 gttcgacagt ttcgtcactg gaaaggccaa ccagctggcg cgtgccgccg ccatccaggt
      481 cgccgagaac cccggcatct cgtacaaccc cctgtttatc tacgggggcg tggggctggg
      541 caaaacgcat ttgctgcagg cgatcggcaa tcacctggcg gaacagcggc cgcaggccag
      601 gctacgctac acgcacgccg aacagtacgt gaccgacgtg gtgcgcgcct accagcaaaa
      661 gtcattcgac gaattcaagc gctactacca ctcgctcgac ttgctgctga tcgacgatat
      721 ccagttcttt tccaacaaag ggcgcacgca agaggaattc ttctacgcgt tcaacgcgct
      781 cgtggaggca cacaagcaga tcgtcatctc ctgcgacacc tatcccaagg aaattgcggg
      841 catggaagag cggctgattt cgcgtttcgg ctggggcttg accgtagcca tcgagccccc
      901 ggagctcgag atgcgcgtcg ccatcgtgct caagaaagca gaagccgaag ccgcgcagat
      961 atcggaggaa atagcctttt tcattgccaa aaacatccgc tcgaacgtgc gcgagctgga
     1021 gggcgccctg aagaaggtcc ttgcgttcgc gcggttcacc gggcgcgaga tcacactgga
     1081 aatggccaag gaagcgctgc gcgacatcct caacgttgcc agtcgccaga tcagcgtcga
     1141 cggcattcag aagaccgttg ccgagtattt caagctcaag atatcggaca tgcattcgaa
     1201 aaagcgcagc cgcaacgtgg cgcggccgcg ccaggtcgcc atgtcgctcg ccaaggacct
     1261 gacaccgatg agcctgcccg agatcggcga ggcgttcggc aatcgtgatc acaccactgt
     1321 gctgcatgcc tgccgcacca tctccatgct ccgcaagaag gattccgtgc tcaatcgaga
     1381 ctacctcgtc ctggaacagg tgctacgggg atgacgtttg ggggaataag cctgtgtatt
     1441 tccggtgagc ggattgtgtg caaaccacat aacctggaaa aagtcgtatt ccgtgcactt
     1501 cgttgcacat gtagtgcaca gcttgtacag cgcgatcaag cgccgcaacc tgatgcgtac
     1561 gcacgtaaaa aggcaggact tgaacaagta ggcctgctct attaattact actaggaaat
     1621 atatatggct ttagtcaaag cgaagcgcga tgaacttctc gggccgttgt ccgcggtcag
     1681 cggcatcatt gagcgtcgtc acacgttgcc gatactgtcg aacgtgttga tcgaccgggt
     1741 tgacgacacc ctttcgtttc tcgccacgga catcgagatc cagatcacgg cccgatccac
     1801 cctgccagcc ggtgcggacg ccaaacccgt aacggtcggt gcccgaaagc tggtcgatat
     1861 cctgcgggcc ttgcccgaca gcacggaagt gaccgtgcag atgcaggaca agcgcctaca
     1921 ggtgaagtcc ggcaagagcc gctttaccct gcaaacactg ccggcggagg attttccgcg
     1981 actggcgcgg ccgagcggtg agatggcgag cttcgcgctg ccgcagaaag cgcttcggcg
     2041 cctgctgggt ctggtgcagt acgcgatggc gcagcaggat attcgctact acctcaacgg
     2101 gcttctggtg ctggtggaag accagcagct gcgtctcgta gccacggacg ggcatcgcct
     2161 cgcctacgca tccatgaagc tgccggcgag cctgccccgg cttgaagtga tcgtgccgag
     2221 gaagacggtg ctcgaactct ccaagctgct gggagacggc gaggaagagg tgaaaatcga
     2281 tctctctggc gcccaggcgg ccttcaacct cggcaccgtg gagctggtca ccaagctcgt
     2341 agacggaaag tttcccgatt acacgcgtgt cataccgacg caacacaaga atctggtgaa
     2401 actcgaacga gaggtgctgc gccaagcact gcagcgagcg gcaattctct cgaacgagaa
     2461 attccgcggc gtgcgctggg tcctgacgga cggcagcctg aagatcgtct cgtccaacac
     2521 ggagcaggag gaggcgcagg aggaattgga ggtggaatac agcggtgacg cgatcgacat
     2581 cggcttcaac gtcaactatc tcctagacgt attgaacaac gtttccggta acgaggtcga
     2641 atgccatttc ggcgatgcgg catcgagcgc gttgctgcag tttcccgcgg agcccgactt
     2701 caagtacgtc gtcatgccga tgcggatata gggaggactc gcatgggtga atccgaacag
     2761 cagcggcccg acggcggcga ctacgactcc gacagcatca aggtcctgaa ggggctggag
     2821 gcggtgcgca agcgcccagg catgtacatc ggggatactt ccgatggaac cggcctgcac
     2881 cacatggtgt tcgaagtggt ggataacgcg gtggacgagg cgctggccgg atactgcgac
     2941 gaaatcatcg tcaccatcca cacggacaat tcgatctcgg tgctggacaa cggacgcggc
     3001 atcccgacgg ggatcaagga ggacgacgag ctcaagcgat ctgccgcgga gatcgtgatg
     3061 acggaactcc acgccggcgg caagttcgac tccaacacct acaaggtgtc gggaggtttg
     3121 cacggggtag gcgtctccgt ggtgaatgcg ctctccagct ggctgcgctt gaccatctgg
     3181 cgcgacgcca gggtgcacca gctcgagttt gccgacggcg cgcccaccgc gcccctgcgc
     3241 gtcaccggga ccagcgagaa gcgcggtacg gaagtgcact tcctggcttc gcgccagatt
     3301 ttcggcaatg tcgactacca ctacgacatc cttgcgcgtc gcctgcgcga gctttcgttt
     3361 ctcaacaacg gcgtgcgaat tcttctggtc gaccagcgca gcggcaaaga agaggacttc
     3421 gcgttcaccg gcggcgtgaa gggtttcgtc gagtacatga accgtgcgaa gaccgtattg
     3481 catcccaacg tgttcagcgc gcagggagag aaggacggcc tcagcgtgga tgtggcgatg
     3541 cagtggaacg attcttacca ggaaaacgtg atttgcttta ccaacaacat cc
//
LOCUS       DASUOM010000393         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_134607,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000393 DASUOM010000000
VERSION     DASUOM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134607"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /gene="glgB"
                     /locus_tag="VFY27_08215"
     CDS             <1..2055
                     /gene="glgB"
                     /locus_tag="VFY27_08215"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5765538.1"
                     /translation="LPRTRRVFLESEQRPMQRIGDTDLFEFVGNTDDLPRHYRLLVDD
                     SFGNRRTQYDPYSFPPLTDEADLTSFNAGTHTAAYRFLGANPKAVAGIDGTLFAVWAP
                     NAERVSVVGDFNEWDGRCHPMRIRGQSGVWELFLPQLLAGPYKFELRNRSTGAVALKS
                     DPFAKFTEGRPATASLVVRESDHEWRDRKWLAARSRRNFMTAPMSIYEAHLGSWKRGE
                     ENRFLTYGELASQLPQYVRDLNFTHVELLPVSEHPLDESWGYQTTGYFSPTSRHGTPD
                     EFRAFVDRCHQQNLGVLLDWVPAHFPRDAHALANFDGTALYEYHDPRKAEHRDWGTLV
                     FNYERNEVRSFLISNALYWLREFHIDGLRVDAVASMLYLDFSRQGENWVPNRLGGNQN
                     LEAIDFICELNKAVGRECPQWLMIAEESTDWQGVTSPTYAGGLGFHLKWNMGWMHDTL
                     DYFEKDPIHRKYHQDWLTFSPTYAFNENFVLPLSHDEVVHLKRSLFGKMPGDEWQRFA
                     NLRLLYTYQWSFPGKKLLFMGGELAQPGEWNASESLPWHRLDEDGPRGISALLADLNQ
                     LQGQCPALAAWDCDPRGFEWLDGDNRDMSIIAFLRNAPEQQAVVVLNFTPTVRHGYRV
                     GVPDEGSYREILNSDLARYGGSNLYNPGDIASENIPWHGRAQSIVLTLPPLAGVILVR
                     SA"
     gene            complement(2118..>2360)
                     /locus_tag="VFY27_08220"
     CDS             complement(2118..>2360)
                     /locus_tag="VFY27_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5765539.1"
                     /translation="ELQKFEQERSEIYIYDLAIAQTHRRQGIATALILELKKIAAARG
                     AYVIYVQADLGDDPAIALYTKLGTREDVLHFDIAVD"
BASE COUNT          468 a          728 c          722 g          442 t
ORIGIN      
        1 ctgccgcgca cgcgccgggt gttcctggag tccgaacagc gtcccatgca gcgcatcggg
       61 gacaccgacc tgttcgagtt cgtcggcaac acggacgatc ttccgcgcca ctatcgcttg
      121 ctcgtcgatg acagcttcgg caacaggcgc acacagtacg atccatactc gttcccgccg
      181 ctgacggacg aggccgacct cacgagtttc aacgccggaa cgcacacggc ggcatatcgt
      241 ttcctcggcg ccaacccgaa ggcggtcgcg ggcatagacg ggacgctttt tgcggtgtgg
      301 gcgccgaatg ccgagcgggt aagcgtcgtc ggcgacttca acgagtggga cggccgctgc
      361 catccgatgc gcattcgcgg ccaaagcggc gtatgggagc tgttcctgcc ccagctgctg
      421 gcgggtccct acaagttcga attgaggaat cgttcgacgg gagccgtcgc gctgaaatcg
      481 gatccatttg caaaatttac agagggccgg ccggcgacgg cgagcctggt cgttcgggag
      541 agcgaccacg aatggcgcga ccgaaagtgg ctagccgccc ggtcgcgccg taatttcatg
      601 acggcgccga tgtcaattta cgaggcgcat ctcgggtcct ggaagcgcgg cgaggaaaat
      661 cgcttcctga cctatggaga actggcgtcg caactgccgc agtacgtccg cgaccttaac
      721 ttcacccatg tcgaactgct cccggtgagc gaacacccgc tggacgaatc ctggggttac
      781 cagacaaccg gctatttctc gcccaccagc cggcatggca ccccggatga atttcgcgcg
      841 ttcgtcgacc gctgccacca gcagaacctc ggcgtgctgc tggactgggt cccggcgcat
      901 tttccccgcg atgcgcatgc gctggcaaac ttcgacggca ccgccctgta cgaatatcac
      961 gacccgagaa aggcggaaca ccgcgactgg ggcacgctgg tattcaacta cgagcgcaac
     1021 gaggtgcgct ctttcctgat ctcgaacgcg ctttactggc tgcgcgaatt ccacatcgac
     1081 ggtttgcgcg tcgacgcagt ggcgtcgatg ctgtacctgg atttttcgcg ccagggcgag
     1141 aattgggtac cgaaccggct cggcggcaac cagaacctgg aagcaatcga tttcatctgc
     1201 gaactgaaca aggcggtcgg ccgcgagtgt ccccaatggc tgatgatcgc ggaggaatct
     1261 accgactggc agggcgtcac cagcccgacg tatgcaggcg gtctagggtt tcacctgaag
     1321 tggaacatgg gctggatgca cgacacgctg gattacttcg agaaggaccc gattcaccgc
     1381 aagtaccacc aggactggct cacgttcagc ccgacctacg cattcaacga aaacttcgtg
     1441 ctgcccctgt cgcacgacga ggtggtgcac ctgaaacgtt cgctgttcgg caagatgccg
     1501 ggggacgagt ggcagcggtt cgcgaacctt cgcctgctgt atacctacca gtggagtttt
     1561 ccggggaaga agctgctctt catgggcggc gaactggcac aacccgggga atggaacgcg
     1621 agcgaatcct tgccctggca ccggctcgac gaagatggac cgagaggaat ttcggcgctt
     1681 ttggcggacc tgaaccagct acagggccag tgcccggcgc tggccgcctg ggattgcgat
     1741 ccccgcggat tcgaatggct ggacggcgac aaccgcgata tgtcgatcat cgcgttcctg
     1801 cgcaatgcgc cggaacaaca ggcggtggtc gtgctgaact tcactccgac ggtgcggcat
     1861 ggctatcgcg tcggcgtccc tgacgaaggc agctatcgcg aaatcctgaa ctccgacctg
     1921 gcacgctatg gcggaagcaa cctgtacaac ccgggcgaca ttgcaagcga aaacataccc
     1981 tggcatggac gtgctcaaag catcgtgctg acgctgccgc cgcttgcggg ggtgatcctg
     2041 gtgcgatcgg cgtagcagcc ggcggagacg tcaccgccgt tccaccagca ccgcccactg
     2101 ccagcgtaat cgaatagtca gtcaaccgcg atgtcgaagt gcaggacatc ttctcgcgtt
     2161 ccgagtttcg tataaagcgc tatcgcggga tcgtcgccca ggtccgcctg tacatagatg
     2221 acataagcgc cccgtgctgc cgcgatcttc ttgagttcca ggatcagcgc ggtggcaatt
     2281 ccctgccgcc gatgtgtttg tgcaatagcg aggtcgtaaa tgtagatctc gcttcgctcc
     2341 tgctcgaact tctgcagctc
//
LOCUS       DASUOM010000394         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1047904,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000394 DASUOM010000000
VERSION     DASUOM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047904"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFY27_08225"
     CDS             <1..1198
                     /locus_tag="VFY27_08225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5765540.1"
                     /translation="LVLQRLARMGDGRLLLEDGVGRNIFGHGAKDGHGAVTLTVLDPR
                     FYTAVASTGAVGAADAYIEGYWTCNDLTGLVRLLLKNREVLEGLEGGLARLAQPVRRV
                     YHWLNRNTRRGSRKNISAHYDLGNDFFKLWLDDTMMYSSAIFEREDMSLHEASVAKLD
                     RISRKLELSPADHVLEIGTGWGGFALHAARNYGCRVTTTTISREQYLYAKARVDDLGL
                     SDRVTLLFQDYRELAGRYDKAVSIEMIEAIGHENLDSYFARCSGLLKEEGMMLVQAIT
                     IADQRYAASLKSVDFIQRYIFPGGFLPSTTAILQSVTYSSDMRLYHLEDIGAHYARTL
                     SCWRERFRAKLSTIRALGYPEKFLRMWEYYYCYCEGAFLERATGTAQLLLVKPGCRRE
                     PLITPL"
     gene            complement(1099..>2140)
                     /locus_tag="VFY27_08230"
     CDS             complement(1099..>2140)
                     /locus_tag="VFY27_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5765541.1"
                     /translation="RASIARHIAISRGLEASPDDVTITNGTQQALDLLARLLLAPGDR
                     VAVENPGYHPPARLFRTLGAQLTGVPVDAEGLVVDSLPRNTRVVYVTPSHQYPLGVTM
                     SMRRRQALLEWAGRTNAAIIEDDYDSEFRFGGRPIEPLYTLDTTGRVVYIGSFSKTML
                     PTLRLGFIVTPPSLSAAAHKAKHVMDWHTNLLLQAALARFIDDGFFARHVRKAGSVYR
                     KRHEMILEILRSHFADVLEVVPSAAGLHITATAKGHSAEEISTVARRASDRDVAVHRL
                     SQFGIDGPAQPGFVIGYGAIATGDIEEGLRRLRACFAGHKGVMSGSRRHPGLTSSSCA
                     VPVALSRKAPSQ"
BASE COUNT          410 a          657 c          664 g          409 t
ORIGIN      
        1 gctcgtgctg cagaggctcg cgcgaatggg cgacggccgc ctactcctgg aagatggtgt
       61 cggtcggaac atcttcggcc acggcgcgaa agacggccac ggcgctgtga ctttaacggt
      121 cctcgatccg cgtttttaca ccgctgtcgc ctccaccgga gctgtcggcg cagcagacgc
      181 atacatcgag ggctactgga cctgtaacga cctcacaggt ttggtccggc tgttgctcaa
      241 gaaccgggag gtgctcgaag ggctggaggg cgggctggcg cgcctggcgc agccggtgcg
      301 acgcgtctat cactggctga atcgaaatac gcgacgcggc agccgcaaga acatatccgc
      361 ccattacgat ctcggcaacg acttcttcaa gttatggctc gacgatacga tgatgtactc
      421 ctcggcgatt ttcgagagag aagacatgtc tctgcacgag gcgtcggtcg cgaaactcga
      481 ccgtatcagc cggaagctcg agctctcccc ggctgaccac gtactggaga tcggtacggg
      541 ctggggcggc ttcgcactgc acgccgcccg taactacggt tgccgggtca cgacgacgac
      601 gatttcacgt gagcagtacc tttatgccaa ggcacgtgtt gacgatctcg gcctgagcga
      661 ccgggtaacc ctgttgttcc aggattatcg cgaactcgcc ggcaggtatg acaaagcggt
      721 gtccatcgag atgatcgagg ccatcgggca cgaaaacctc gattcctatt tcgcccgctg
      781 ctcggggctc ctcaaggagg agggcatgat gctggtgcag gccatcacca tcgcggacca
      841 gcgctatgcg gcttcgctta aatcggtcga cttcatacag cgctacattt tcccgggcgg
      901 attcctgccg tcgacgaccg cgatcctgca gtccgtaacg tactcgtcgg acatgcggct
      961 ctatcacctc gaagacatcg gcgctcatta cgcccgaact ctgagttgct ggcgcgagcg
     1021 attccgcgcg aagctttcga cgatccgcgc gctcggctat cccgagaaat tcctgcgcat
     1081 gtgggagtac tactactgct actgcgaagg agccttcctg gagagggcga ccggaacggc
     1141 acagctactg ctggtcaagc ccggatgccg tcgggagcca ctcatcactc ctttgtgacc
     1201 ggcgaaacag gcgcgcaagc ggcgcagccc ctcttcgata tcgcctgtgg cgatagcgcc
     1261 gtagccaatt acgaatccag gttgcgccgg accgtctatt ccgaactgcg acaaccggtg
     1321 cacggcaacg tccctgtccg aagcgcgccg cgcaaccgtg gatatttctt ctgccgaatg
     1381 acccttcgct gtcgcggtta tgtgcaatcc cgcagcggag ggaacgacct ccagcacgtc
     1441 cgcgaagtgg ctccgcagga tctcgaggat catttcgtgt cgcttccggt aaacgctacc
     1501 cgccttgcgg acgtggcgcg caaagaaccc gtcgtcgatg aagcgagcca gcgctgcctg
     1561 caacagcaag tttgtgtgcc agtccatcac gtgtttggcc ttgtgtgctg ctgcgctcag
     1621 cgagggtggc gtcacgatga agccgaggcg aagtgtcggc agcatggttt tggagaacga
     1681 gccgatgtac accacgcggc cggtggtatc gagcgtatag agcggttcga tcggtcggcc
     1741 gccgaagcga aactcgctgt cgtagtcgtc ttcgatgatc gctgcgttcg tgcgtccggc
     1801 ccactccagc aacgcctggc gccggcgcat gctcatcgtc acaccaagtg gatactggtg
     1861 cgatggggtg acgtagacaa cgcgggtatt acgcggcaat gaatcgacga ccagtccctc
     1921 ggcgtcaacg ggcacgccgg tcagctgcgc tccaagtgtg cgaaacaggc gagcgggagg
     1981 atggtagccc gggttctcca cggcaacgcg gtcgcccggc gccagcaaga gccgcgccag
     2041 caggtccagg gcctgttgcg tcccattcgt aatggtgacg tcatccggcg acgcctccaa
     2101 cccgcgtgaa atggcaatgt gtcgcgcgat cgatgcacgg
//
LOCUS       DASUOM010000395         6471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_272503,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000395 DASUOM010000000
VERSION     DASUOM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272503"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFY27_08235"
     CDS             complement(<1..1036)
                     /locus_tag="VFY27_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5765542.1"
                     /translation="MFRATTFMIGLALAAAPAFAEEQPPEPAPGLTGYKAEAAAQIDR
                     MQKLTQEVVDSVFSFAELGFQEFETQTYLTSILEDNGFTVEQGVSGIPTAWWATWGSG
                     KPVIALGSDIDGIPKSSQMPGVAWRQPVIEGAPGHGEGHNSGQAVNITAALAVKEIMR
                     REGLPGTIVVWPGVAEELLASKAWFARDGHFDGVDAVLFTHVSDNMAVSWGRPRGTGL
                     VSVEYTFDGLAAHGAGDPWKGRSALDAVELMNVGWNYRREHLHPLQRSHYVIVDGGDQ
                     PNVVPSRATVWYFIREIEADGIRRNFATLQQIAEGAALMTDTTVTRRIVGAAYPRHFN
                     KPVALAMDRNI"
     gene            complement(1232..2314)
                     /locus_tag="VFY27_08240"
     CDS             complement(1232..2314)
                     /locus_tag="VFY27_08240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5765543.1"
                     /translation="MPLVSACAVPGATSDPRYFTPSGLPLRRVNVARDRIIRTVTGFR
                     PYRNSGFVVRAHKLHDKIVVHNYGHGGGGITLSWGTSQMALELARQTGFRRCAVIGCG
                     AVGLASARLLQQDGWDVTIYARDLPPQTTSNVAGAQWSPASVFEENSVGSAFHARLRT
                     AMEHSYRRFQGMVGDRYGVRWVSNYMLSDRPFRSDDLFARHSDMYPEFRTLAGDQHPF
                     PASYARHFDTMFIEPPVYLQAVMQDYYAAGGSIVVREFRDSSELLSLEEPVLVNCSGL
                     GSRVLFGDDELVPVKGQLTVLMPQVGINYLMIYQGIYMFPRADGVLLGGTFQRNVSTP
                     EPDERESERILCAHRAFFNGMADPWS"
     gene            2539..3264
                     /locus_tag="VFY27_08245"
     CDS             2539..3264
                     /locus_tag="VFY27_08245"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX5765544.1"
                     /translation="MKSVGRIAGVLGGMGPDATIDFMSKVIALTGGRSDQDHIRMIVD
                     HNPKVPDRQAAIFEGAEDPGPALARMAKGLESAGADFLVIVCNSAHAFLDPVRQATTL
                     PLVSIIDVTLEEVSRSCPRAKRAGLLATDGLIASGIYQDALEAAGLEPVLPREEDLKT
                     LMVLIRKIKAGDRSREVAQEMGTLAELLASAGADIIIAGCTEIPLVLNAAVITVPVIS
                     STDALARKTVALARGVEALDPLP"
     gene            3307..4305
                     /locus_tag="VFY27_08250"
     CDS             3307..4305
                     /locus_tag="VFY27_08250"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEX5765545.1"
                     /translation="MLLSRHPRISLAHLPTPLEFLPRLTKHLGGPKIFVKRDDCTGLA
                     TGGNKTRKLEFLLGDALQKGATSVITAGAVQSNHARQTAAAAAKCGLACTIVFEHRLE
                     GASESYLKSGNVLLDKLFGARIRNVAKDTDTMAEMEAVAEELSEDGEVAYIIPGGGSN
                     PIGALGYVNCAFELMMQANQQGLVVDHVVHATGSAGTQAGLAAGLKAVHADVPLLGIG
                     VGAPREEQEQRVFDLAEQTADFIGAPGTVRREDIVCNCDYVGPGYGVPTDAMNSAVLL
                     LAQLEGLLFDPVYTGKGMAGLIDLVGNGWFEGAENIVFIHTGGSAGLFAYADQLRT"
     gene            complement(4405..5688)
                     /locus_tag="VFY27_08255"
     CDS             complement(4405..5688)
                     /locus_tag="VFY27_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008896979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5765546.1"
                     /translation="MRIRGILISCCAFISASASAETLIHAGRVIDGISDRARTEMTIR
                     IDGGQIVAVERGYAAPGQQDLLIDLKEETVLPGLMDAHVHLTGEYSKDDRLNDFIMNE
                     ADYAFDAAKFAKRTLEAGFTVVRNLGDSFNVTIALRAAIESGDIPGPRIITSAKGLSS
                     TGGHGDPTNGWADRIKGDPGPVDGVVNSVEDARKAVRQRYKDGADWIKITATGGVLSV
                     AKSGQNPQFTDEELSAIVDTAADYGMRVAAHAHGTEGMKRAVVAGVASIEHGTYMDAE
                     VMQLMKENGTYYVPTILAGNWVAEQAKIDGFFPELVRPKAAAIGPIIQGTFTKAYEAG
                     VAIVFGTDTGVSAHGDNAQEFALMVEGGMPPMEAIKAATSVAARFLDIGETHGTLEAG
                     KQADIIAIPGNPLENIRLLEDVTFVMKGGKVHKQP"
     gene            5881..>6471
                     /locus_tag="VFY27_08260"
     CDS             5881..>6471
                     /locus_tag="VFY27_08260"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX5765547.1"
                     /translation="MAGPARAAEQWSSKIGFLLATIGFSVGLGNIWRFPYLTGENGGS
                     AFLFVYLACAFGIGLPLLISELAIGRRGRRSASGSIRAVAAESGVSSRWGAVGGLAVL
                     CVFIILSYYSVITGWTLDYFLRAASGKFTNIDAAGSTDMFAGLLSNPLRLLFWNTVVH
                     ILIFLVVRRGVQGGVERANKILMPALFISLVVMVAYA"
BASE COUNT         1128 a         2058 c         1991 g         1294 t
ORIGIN      
        1 ggatgttccg gtccatggcc agcgcgaccg gcttgttgaa atgtctcggg taagcggcac
       61 cgacgatccg ccgggtgacg gtggtgtcgg tcatcaatgc cgcaccctcg gcgatctgct
      121 gcagggttgc gaaattgcgg cgaatcccgt ctgcttcgat ctcgcgaatg aagtaccaga
      181 ccgtcgccct cgaaggcacg acgttcggct ggtcgccgcc atcgacgatg acgtaatgcg
      241 agcgctgcag cggatgcagg tgctcgcgac ggtaattcca gccgacgttc atcagctcga
      301 ctgcgtcgag ggcgctgcgg cctttccatg gatcgcctgc cccgtgggcc gcaaggccgt
      361 cgaacgtgta ctcgaccgat accagtcccg ttccgcgcgg cctgccccag ctgacggcca
      421 tgttgtccga cacatgcgtg aaaagcaccg catcgacgcc gtcgaagtgt ccgtcgcggg
      481 cgaaccatgc tttgctggcg agcagttcct cagcaacacc aggccacacc acgatcgtgc
      541 cgggcaagcc ttcccgccgc atgatctctt ttaccgcgag cgcggcagtg atgttcacgg
      601 cctggccgga gttgtgtccc tcaccgtgac ccggtgcgcc ttcgatgacg ggctggcgcc
      661 acgccacgcc gggcatctgg gaagatttgg gaataccgtc gatgtccgat cccagtgcga
      721 tcacaggctt gccgctgccc caggtcgccc accacgccgt cggtatgccg gacacgccct
      781 gctccacagt gaagccgttg tcctctagga tcgacgtgag gtaggtttgc gtctcgaact
      841 cctggaagcc cagctctgca aaggaaaata cgctgtcgac cacctcctgc gtgagcttct
      901 gcatgcggtc gatctgcgcg gcggcttcgg ctttgtagcc tgtcagcccc ggtgcgggct
      961 ctggcggctg ttcctccgcg aaggccggcg cggctgccag cgccaggcca atcatgaatg
     1021 tcgtagctcg aaacattacg tccccccgtt cgaccgcttc tttgttttcc tggatcgccc
     1081 cctgatgccg cgtggccgcg tgagccgggc cggcgttgtc aaggggcggg cgaccctttg
     1141 ccgcctactg tacccggctc ctactggtgt ggtcacttcc gcggcaacat gtgcaagacg
     1201 tttctagagc ctgctcgcat gggaagtacc gtcagctcca cggatctgcc atgccgttga
     1261 aaaaagcgcg gtgtgcgcat aggatgcgct cgctttcgcg ctcgtcgggc tccggcgtcg
     1321 acacgtttcg ctgaaacgtt ccgccgagca gcacgccgtc cgcgcgtggg aacatgtaga
     1381 tcccctggta gatcatgagg tagttgatgc caacctgtgg catcaacacc gtcagctggc
     1441 ccttcaccgg cacgagctcg tcgtcgccga acagcacacg cgagccgagt ccgctgcagt
     1501 tgacgagcac gggctcttcg agcgagagca gctcgctgct gtccctgaac tcgcgcacga
     1561 caatgctgcc tccggcggca tagtagtcct gcattactgc ctgcaggtat accggcggct
     1621 ctatgaacat cgtgtcgaag tgcctcgcgt acgatgcagg aaagggatgc tggtctccgg
     1681 cgagcgtgcg gaactccggg tacatgtccg agtgacgcgc gaaaaggtcg tcggacctga
     1741 agggacggtc agacagcatg tagttgctca cccagcgcac tccgtaacgg tcgccgacca
     1801 taccctggaa acgccggtac gaatgctcca ttgcggttcg cagtcgggca tgaaaggcgc
     1861 tcccgaccga gttctcctcg aaaacggagg ccggcgacca ctgcgcaccg gcgacgttcg
     1921 acgtcgtctg cggcgggagg tccctggcat agatcgtcac gtcccacccg tcctgctgca
     1981 gcaagcgcgc gctggcgagg ccgacggcgc cgcaaccgat gacggcgcag cgcctgaacc
     2041 cggtctggcg cgcgagctcc agcgccattt gcgaggtgcc ccaggaaagg gtaataccgc
     2101 ctccgccatg cccataattg tgcacgacga ttttgtcgtg gagcttgtgc gcgcggacga
     2161 caaagccgga attgcggtaa ggccggaagc cggtgaccgt tcgaatgatg cgatctctcg
     2221 ctacgttgac gcggcgcaac gggagcccgg acggtgtgaa gtatcgagga tcgctcgtcg
     2281 cccccggtac tgcgcatgca ctgaccagcg gcatcgagat ggcggctgca atgaagttgc
     2341 gccgggagat cggcggtgct tttatcgtta tagtgctgat cgtcgttccc tgcccgaact
     2401 atgtccggct aagacgccat tcgggaattc gtttactctt ggctgcacgg actccccggt
     2461 gctgaaccgg atgttaacca ttttcgtcga cctgtgtccc accctggcca gctacatgaa
     2521 aatccaatac cgtcgcatat gaaatctgta ggacgcatcg ccggcgtgct cggaggcatg
     2581 ggacccgatg cgactatcga cttcatgtcg aaagtaatcg ccctgaccgg cggaagatct
     2641 gaccaggatc acattcgcat gatcgtcgac cacaatccca aggtacctga ccgccaggca
     2701 gccatattcg aaggcgcgga ggatcccggt cctgcccttg cccgcatggc aaaggggttg
     2761 gagtcggcgg gtgccgattt tctggtcatc gtgtgcaatt ccgcgcacgc tttcctggat
     2821 ccggtccggc aggccacgac cctcccgctc gtcagcatca tcgacgtcac gctggaggaa
     2881 gtttcgagaa gttgcccgcg tgcgaagcgt gcaggactgc ttgccaccga cggcctcatc
     2941 gcgtccggca tctaccagga tgcgctggag gcggcgggcc tggaaccggt gttgccgcgg
     3001 gaggaggacc tgaagaccct catggtcctg attcgcaaga tcaaggctgg agacaggagc
     3061 agggaagtgg cgcaggagat gggcacgctc gccgagttgc tggcttcggc aggcgccgac
     3121 atcataatcg ccggctgcac cgagatcccg ctggtcctta atgccgcggt cattaccgtg
     3181 ccggtaatct cgtccaccga tgcgctcgcg cgcaagaccg tcgcgctggc ccgcggtgtc
     3241 gaggcactcg atcctttacc ttgatccttt agtcccggat cagaactgac ctactggaga
     3301 atcctcttgt tgctttcgcg ccatcccagg atctcactgg cccatctccc cacgccgctc
     3361 gaatttcttc cccgcctgac aaagcacctc ggcggcccca aaattttcgt caagcgggac
     3421 gactgcaccg gccttgccac cggcggcaac aaaacccgca agctcgagtt cctgctgggt
     3481 gatgcgctgc agaaaggggc gacatccgtc atcaccgcgg gcgccgtgca gtcgaaccat
     3541 gcgcgacaga ctgctgccgc ggcggcgaag tgcggccttg cgtgcaccat cgttttcgag
     3601 caccgcctcg agggagcaag cgagtcctat ctcaagtccg gtaacgttct cctggacaag
     3661 ctgttcggcg cgcgcatccg caacgtggcc aaagatacgg acacgatggc ggagatggag
     3721 gcagtggccg aagagctcag tgaggacggg gaggtcgctt acatcattcc gggcggtggt
     3781 tccaacccga tcggagcact gggttacgtc aactgcgcct tcgagctgat gatgcaggct
     3841 aaccagcagg gactcgtcgt cgatcatgtc gtgcacgcga ccggaagtgc aggaacgcag
     3901 gcgggactcg cggccgggtt gaaagccgtc cacgcggatg ttccgctgct cggcatcggc
     3961 gtcggtgcac cgcgtgaaga acaggaacag cgggtgttcg acctggcgga acaaaccgcc
     4021 gacttcattg gcgcccccgg cacggtacgg cgtgaggaca tcgtctgcaa ctgcgactat
     4081 gtcggaccgg gttatggcgt ccccactgat gcaatgaatt cggcggtcct cttgcttgcg
     4141 caactggaag gcctgttgtt cgatcccgtc tacaccggca agggcatggc aggcctgatc
     4201 gacctggtcg ggaacggatg gttcgagggt gctgaaaaca tcgtgttcat acacaccggg
     4261 ggctccgccg gcctgttcgc gtacgccgac cagctgcgta cctgaggcgc catctccccc
     4321 ggaatagtag ccgtaggagc ccgccacgcg ggcgattccg cctcggcgaa ctcgcccgca
     4381 tggcgggctc ctgcggtgcc aacttcaagg ctgcttgtgg acttttccgc ccttcatgac
     4441 gaaggtgacg tcctccagca gccggatgtt ctcgagcggg tttcccggta tcgcgatgat
     4501 gtcggcctgc tttccggctt cgagggttcc gtgggtttcg ccgatatcga ggaagcgcgc
     4561 ggcgacgctc gttgcggcct tgatcgcctc cattggaggc ataccgccct caaccatcag
     4621 cgcgaattcc tgcgcattgt cgccgtgagc cgatacgccg gtgtctgtgc cgaaaacaat
     4681 cgctacgccg gcttcgtaag cctttgtgaa cgtcccctgg ataatcggac cgatcgccgc
     4741 ggcttttggc cgtaccagct ccgggaaaaa gccgtcgatc ttcgcctgct cggctaccca
     4801 gttcccggca agaatcgtcg gcacgtagta cgtgccgttc tccttcatga gttgcatcac
     4861 ctcggcgtcc atgtaagtgc cgtgctcgat tgaagcgacg cctgcgacca cggcgcgctt
     4921 catgccctcc gtgccgtgag catgggcggc gacccgcata ccatagtccg cggccgtatc
     4981 gacaatcgcg ctcagctctt catccgtgaa ctgcgggttc tgcccgctct tggcgacgct
     5041 cagtacgccg ccggtcgccg tgatcttgat ccagtccgcg ccatccttgt aacgttggcg
     5101 aacagccttg cgggcgtcct cgacgctgtt cacgactccg tctaccggac caggatctcc
     5161 cttgatgcga tccgcccagc cattggtcgg gtcgccatgg ccgcccgtgg aactcagtcc
     5221 ttttgccgag gtgattatcc tggggccggg gatatccccg ctctcaatcg ctgcacgcag
     5281 cgctatggtc acgttgaagg agtccccgag gtttcgcacg accgtgaagc cggcctccag
     5341 cgtccgcttc gcgaacttcg ctgcatcgaa tgcgtaatcg gcttcattca tgatgaagtc
     5401 gttgagcctg tcgtccttcg agtactcccc ggtcaggtgc acgtgcgcgt ccatcaggcc
     5461 gggcaggacc gtctcctctt tcaggtcgat cagcaggtcc tgctgtcccg gggctgcata
     5521 gcctcgctcc acggcaacga tttgcccgcc gtcgatcctg atcgtcatct ccgtccgggc
     5581 acggtcactg atgccgtcga tcacgcggcc ggcgtgaatg agcgtttccg cacttgcgga
     5641 cgctgaaatg aaggcgcagc aggagataag gatgccccgg attctcataa gcgtcggtcc
     5701 ccgtcggtaa tttatgatcc gcggcatgtt accaccccgt gcaggggttc ctgccgcttg
     5761 gacaaccgcc gctgaagtca tgctggctat cgccgcagaa atcacctttg attgtgcgag
     5821 aatgccgcga ctagcggaag gcaccggaaa gcggccacaa gaaaaccagg ggggagctat
     5881 atggcgggac cggcgcgtgc ggccgagcag tggtcatcca agatcggctt tctcctcgcc
     5941 accatcggct tttcggtcgg tcttggcaat atctggcgct ttccatacct caccggggag
     6001 aacggcggca gcgccttcct gttcgtatac ctggcttgcg cgttcggtat cgggctgccc
     6061 ctgctgatca gcgagctggc catcggcagg cggggccggc gcagcgcctc cgggagcatt
     6121 cgcgcggttg ccgcggaatc cggtgtcagt tcccgctggg gagcggtggg tggcctcgca
     6181 gtactctgcg tgttcatcat cctgtcctac tacagcgtca taaccgggtg gaccctggac
     6241 tatttcctgc gtgctgcttc cggcaagttc acgaacatcg acgcggccgg gtccaccgac
     6301 atgtttgcgg gtctcctgag caatccgctg cgcctcctgt tctggaacac cgtagtgcac
     6361 atcctgattt ttctcgttgt gcggcggggg gtgcagggag gggtggaacg ggcaaacaag
     6421 atcctgatgc cggccttgtt catctcactg gtcgtgatgg ttgcctacgc g
//
LOCUS       DASUOM010000396         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_709413,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000396 DASUOM010000000
VERSION     DASUOM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709413"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFY27_08265"
     CDS             <1..517
                     /locus_tag="VFY27_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765548.1"
                     /translation="PRAALADDGAADHSTLERGEYLVTTSGCNDCHTPFKLGPNGPAP
                     DMDRMLSGHPQDLEMPDPPELPEGPWAIVAAATNTAWAGPWGVSFTANLTPDKETGLG
                     KWTLRNFIETIRTGRHLGRGRAILPPMPIPMYKHMTDEDLEAIFTYLQSIKPIENEVP
                     EPRPPAGWDEM"
     gene            606..1154
                     /gene="mug"
                     /locus_tag="VFY27_08270"
     CDS             606..1154
                     /gene="mug"
                     /locus_tag="VFY27_08270"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX5765549.1"
                     /translation="MRRPTREDLQSATGRTVPDVIAHNLDVLFCGINPGLYSGATGHH
                     FARPGNRFWPALYGAGFTTRLLDPSEEHLLLEAGCGITNLVPRTTATAAELTKAELAD
                     ARHALERKVQRYKPLWLAVLGIGAYRIAFDRRDAHIGKQPERLGSTEVWVLPNPSGLN
                     AHYQLPDLIRLFAELRCVNASS"
     gene            1352..2521
                     /locus_tag="VFY27_08275"
     CDS             1352..2521
                     /locus_tag="VFY27_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765550.1"
                     /translation="MSISLYMALVGFGVMVGIPVISTAWVNLLGFTEVEVGRVAGADL
                     GGLALGAATASLFIATVSRRALVLAAIAIAVTANSLCMVLLEYDQVLWLRLLAGFGSG
                     IYTSVAVATLGATAKPARAFNLMLFGFAFSQALELHMLPQLPMNGIYLVFIACYALTL
                     PFLGWLPPRPVDKHQVVGAAINIDAGNDTSEEPGTVPAYAPWLVLGAIVATYINIGAY
                     WTYIELAAADRTISTADGDWVGQVLVWSSFLSIVGCLFATITSNRFGLARPLLVTLVT
                     HSIVVGMLVGGINNVNILVSVFMFNFLWIFVDVYQMATVANIDYSGRFASLMPAAQGL
                     GQLIGPNAAATVLAYGFGYRGIFILCASATMTAMLLYATMYLRLLRRAPALANAS"
     gene            complement(2516..3169)
                     /locus_tag="VFY27_08280"
     CDS             complement(2516..3169)
                     /locus_tag="VFY27_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5765551.1"
                     /translation="MALIWRGRFCDTLLPGRATTTFDREEVIYELGEKERTLFFIQRG
                     LVKIGTIINSGREIIYDIRKDGDVVGELSCFETSRRDRAVAIERTEAVPVPFTEAMHV
                     LAQNPALLQDFIEACGRALSEAYDQVNRLADGSVERGLVEVLQSLAEKLGHPAGQFVE
                     IGAYLTQEELSQMVVARRERVSTALNSLRRRGIVQYSARGFLLVDTSALESYNSPSL"
     gene            complement(3284..4630)
                     /locus_tag="VFY27_08285"
     CDS             complement(3284..4630)
                     /locus_tag="VFY27_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765552.1"
                     /translation="MKTYRKYVMAFCALVVAFSGYSIARAEPEQQSAQQRHGQSDRHA
                     QRMLDEGQRIFRFDTFGDEAFWGDTLRLHEAIAGRANGGVGPGVSPETALAVGLKVDV
                     EALPHKLRNELRKGRVDLADPATTLALLQLDAVVGVRGFFDDTGQSLQSVGITCALCH
                     SSVDDSLAPGIGHRLDGWAARDLDVGAIIALSPNVQPFIDLLGLVDPTTDAATVRAVL
                     RSWGPGKFDAELLMDGKAFRPDGKSAATLIPPAFGLAGVNLHTWTGWGSITHWNAFVA
                     VLEMQGQGTFFDPRLNDAVKFPIATLAGFGDVRRKPDLVTAKLGPLQFYQLSLPAPEP
                     PRGSFHAAAAKRGEALFDGKADCARCHVPPLYTEPGWNMHRPEEIGIDGFQAERSPDE
                     HYRTAPLKGLWTHTRGFYHDGRFATLNEVVDHYDTHFRLLLSSREKRDLVEFLKSL"
     gene            complement(4807..>5183)
                     /locus_tag="VFY27_08290"
     CDS             complement(4807..>5183)
                     /locus_tag="VFY27_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765553.1"
                     /translation="RAAMPSDRLSAMDGEVAIHPVVDSVTLRVGNTVKLLNIGAGPHA
                     SASLGVLAVEQGLFFQSDLHVPNSESDTPREDRVVTECWFARWAVEHLPPKTIVLNSH
                     TSPRTPVSRLAKYVASPECQAL"
BASE COUNT          965 a         1698 c         1537 g          983 t
ORIGIN      
        1 cccccgcgca gccttggccg acgacggggc cgccgaccac tcaacacttg agcgcggcga
       61 gtacctcgta accacctccg gctgcaacga ctgccacacg cccttcaaat tggggccgaa
      121 cggccccgcc cccgacatgg accgcatgct gtccgggcat ccgcaggacc tcgagatgcc
      181 ggacccgcct gagttgcccg aaggtccgtg ggcaatcgtc gccgctgcca ccaacactgc
      241 ctgggcgggt ccgtggggtg tcagcttcac ggcaaacctc actccggaca aggagaccgg
      301 actcggcaaa tggacgctgc ggaacttcat cgaaacgatt cgtaccggtc ggcatctcgg
      361 ccgcggccgc gcgatcctgc cgccgatgcc gatccccatg tacaagcaca tgacggacga
      421 agacctggag gcgatattca cctacctgca gtcgatcaag ccgatcgaaa acgaggtgcc
      481 ggagccgcgt ccgccggccg gttgggacga aatgtagcca ggatcggccc gcaccgccca
      541 aagaccgaat agaagcgcac tcgcctcatg gcaggctcct gcatcgctgg ctgtatcatc
      601 cgcccatgcg gcgaccaacc cgggaagacc tgcagtcagc caccggacgc acggttcctg
      661 acgtgatcgc ccataacctc gatgtactct tctgcggcat caaccccggc ctctactccg
      721 gcgcgacggg ccaccatttc gcccgtcccg gcaaccgctt ctggccggcg ctctacggtg
      781 caggattcac aacccgcctg ctcgacccct ccgaggagca cctgctgctg gaagcaggct
      841 gcgggatcac gaacctcgtc ccccgcacca ccgccacggc agcggaactg accaaagccg
      901 aactggccga cgcgcggcac gcgctggagc gcaaggtgca acgctataaa cccctctggc
      961 tagctgtcct cgggatcggc gcttaccgaa tcgcgttcga tcgccgcgac gcgcacatag
     1021 gtaaacagcc cgagcggctg ggttcaacgg aagtttgggt cctgccgaac ccgagcggcc
     1081 tcaacgcgca ttaccaactc cccgacttga tccgactctt tgcggagcta cgctgcgtga
     1141 acgcttcgag ctagaaccgt gccacaccac aggctggctg catgcaagcg cttctcattc
     1201 cgtaaatccc tccgcggacg caccaaaggt tgtctagaat agaacgtctt ctcgccgcct
     1261 ttcgggtggc gaagcgctaa aaacaaaagt gctatcggaa ccaggcccca gacacatcca
     1321 gctacacacc gtcttcgacg gctggcgctc gatgtcgatc tcgctttaca tggcgctcgt
     1381 cggcttcggc gtgatggtcg gaataccggt catcagcacg gcatgggtga acctgctcgg
     1441 gttcacggaa gtggaggtcg gccgtgttgc cggggcagat cttggcgggc tggcgctcgg
     1501 ggcggccacg gcttccctgt tcatcgcgac cgtgagcagg cgcgcgctgg tgctcgctgc
     1561 cattgcgata gcagtcactg caaacagtct ctgcatggtg ctgctcgagt acgatcaggt
     1621 gctgtggctc aggctcctgg ccgggttcgg tagcgggatc tatacgtccg tcgcagtcgc
     1681 gacgctcggc gccacggcca agccggcgcg cgcattcaac ctgatgctgt tcggcttcgc
     1741 gttttcgcag gcactcgagc tgcacatgct gccgcagctg ccgatgaacg ggatctacct
     1801 cgtgttcatc gcctgttacg cgctgacgct acccttcctc ggctggttgc ccccgcgacc
     1861 ggtggacaag caccaggtcg tcggagccgc catcaacatt gacgccggga acgacaccag
     1921 tgaggagccg ggcacggtac ctgcttatgc gccctggctc gtcctcggcg cgatcgtcgc
     1981 cacctacatc aatatcggtg cctactggac ctatatagaa ctcgcggccg ccgaccgaac
     2041 gatttcgacg gccgacggcg actgggtcgg gcaagtgctg gtctggtcct cgttcctgag
     2101 catcgtaggg tgcctgttcg caacgatcac cagcaaccgc ttcgggctcg cgcgccccct
     2161 gctggtcacc ctcgtcacgc attcgatcgt cgtcggcatg ctggtcggcg gcatcaacaa
     2221 cgtgaacatc ctggtcagcg tgttcatgtt caacttcctg tggatcttcg tcgacgttta
     2281 ccagatggca acggttgcca acatcgacta ctccggccgt ttcgcatcgc tcatgccggc
     2341 ggcgcagggc ctcgggcagc tgatcggtcc gaatgccgcc gcgaccgtgc ttgcctacgg
     2401 gtttggctat cgcggtattt tcatcctgtg cgcttcggcg acgatgaccg cgatgctgtt
     2461 gtacgcgacg atgtacctgc ggctgctgcg gagagccccc gcgctggcca acgcatcata
     2521 gtgacggtga gttgtaactc tcgagcgccg aggtatctac gagtaaaaaa ccccgtgccg
     2581 agtactgcac tattcccctg cgtcgcaggg agttgagggc cgtcgaaacc cgttcccgtc
     2641 gtgccaccac catctgcgaa agttcttcct gcgtcagata tgcaccaatc tcaacgaatt
     2701 gaccagcggg atgcccgagc ttttcagcga gagactgcaa gacttccacc agcccgcgtt
     2761 cgacgctgcc gtcggccagg cggtttacct gatcgtaagc ttcagaaagc gccctgccgc
     2821 aggcttcgat gaagtcctgt agcagggccg ggttttgtgc caggacgtgc atcgcttcgg
     2881 tgaaaggcac cggaaccgct tccgtccgtt cgatcgccac ggcacggtcc cggcgtgaag
     2941 tctcgaagca ggaaagctca cccacgacat cgccgtcctt tcgaatgtcg tagataattt
     3001 cccgaccgct attaataatc gttccgatct ttactaaccc gcgctggatg aagaacagag
     3061 ttcgctcttt ctcgccgagc tcatagatga cctcttcccg atcgaacgtc gtggttgcac
     3121 ggcctggcaa tagtgtgtcg cagaacctgc cccgccatat gagggccatc gctgatgaca
     3181 aggcggccac acggagaacg gtatcgttga tcggcatggg tgcatggatg cctgatgacg
     3241 gcccggggcc ctgacgacag tgtcaagtat agcggatcgt cggttacaag gacttcagaa
     3301 attcaaccag atcacgcttc tcgcggctgc tcagtagcag acggaagtgt gtgtcgtagt
     3361 gatcgacaac ctcgttgagt gtggcgaacc ggccgtcgtg atagaagccc ctggtgtgcg
     3421 tccacagtcc cttgagcggc gctgtccggt aatgctcgtc cggtgagcgc tctgcctgga
     3481 agccgtcgat gccgatctcc tccggccggt gcatgttcca gccgggctcg gtatagagcg
     3541 gcggcacgtg gcagcgagcg cagtcggcct tgccgtcgaa caatgcttcg ccgcgttttg
     3601 ccgccgccgc gtggaagctc ccgcgtggcg gttctggcgc aggcaggctg agctgataga
     3661 actgcagcgg cccgagcttc gcggtgacca gatccggttt ccggcgaacg tcgccgaaac
     3721 cggcaagcgt ggcgatggga aacttgacgg catcgttcaa gcgggggtcg aagaaggttc
     3781 cctgcccttg catctccaga acagcgacga aggcgttcca gtgcgtaatg gatccccagc
     3841 cggtccaggt atgcaggttg actccggcca ggccaaaggc gggcggtatc agtgtcgccg
     3901 ccgacttgcc gtccggccgg aacgccttgc cgtccatcag cagctccgcg tcgaacttgc
     3961 ccggtcccca gctccgcagc acggcgcgaa cggtcgcggc gtctgtcgtc ggatcgacca
     4021 gcccaagcag gtcaatgaac ggttgcacgt tcggcgacag cgcaatgatc gcgcccacgt
     4081 cgaggtcccg cgccgcccag ccgtccagcc ggtgaccgat gcccggagcc agtgaatcgt
     4141 cgacactcga gtgacacaga gcacaggtga tgccaacgga ctgcagactt tgtccggtgt
     4201 catcgaagaa tccgcgtaca cccaccaccg cgtccagctg caacaacgcc agcgtcgttg
     4261 ccggatcggc aagatcaacc cggcctttgc gcaactcgtt acgcagcttg tgtggaagcg
     4321 cctcgacatc gaccttgagc ccgaccgcga gcgccgtctc cgggcttacg ccagggccga
     4381 cgccaccgtt ggcccttccc gcaatggctt cgtgcaaacg cagcgtatcg ccccagaagg
     4441 cttcatcgcc gaaggtatcg aagcgaaaga tcctctgtcc ttcgtccagc atgcgctgtg
     4501 catgccgatc gctttgtccg tgtcgctgct gcgccgattg ctgctccggt tccgctcggg
     4561 cgatcgagta cccggaaaat gccacgacca atgcacagaa cgccatgacg tactttcgat
     4621 atgtcttcat tacgacctcc gcgcgttgcg gttcgagaca gcgggaattt ccgcaaccgt
     4681 tcaactgttg ttaaaatcct atacacgcgc gttaatcacg tgtcagttac cgcagtaaca
     4741 cacgaaggta aacggaaccg ttattttccc gacagtccgc tgccttcctc cgctaccccg
     4801 tgttcatcac agtgcctggc actccgggct cgcgacgtat ttcgcaagcc gcgacacggg
     4861 tgtgcgcgga gacgtgtggg aattcagcac gatggttttg ggcggcaggt gttcgaccgc
     4921 ccatcgtgcg aaccagcatt cggtgacaac gcggtcttcg cgcggcgtat cggattcgga
     4981 gttcggcacg tgcaggtcgg actggaaaaa caaaccctgc tcgacagcca gcacgccgag
     5041 cgatgcggag gcgtgcggtc cggcgccgat gttgagcagc ttgacggtgt tgccgactcg
     5101 cagcgtgacg ctgtccacca ccggatgtat cgccacttcg ccgtccatgg cgctcaacct
     5161 gtcggacggc atggcagcgc ggt
//
LOCUS       DASUOM010000397         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1414495,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000397 DASUOM010000000
VERSION     DASUOM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414495"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFY27_08295"
     CDS             complement(<1..180)
                     /locus_tag="VFY27_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765554.1"
                     /translation="MAPGIGPVAQAQEGANNEEIEEIITTGTRRAERTVTESSVPIDV
                     ISGEEFENMGTADMDD"
     gene            complement(522..2201)
                     /locus_tag="VFY27_08300"
     CDS             complement(522..2201)
                     /locus_tag="VFY27_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX5765555.1"
                     /translation="MQSVMIVVLGIIGMSFGWFVYSKFIATKIFRLDPDFVTPAHQIN
                     DGVDFVPTNKYVLWGHHFTSVAGAAPIVGPAIAVYWGWVPAMLWVVLGTIFFAGVHDA
                     GALWASNRHRGKSIGALSEAIIGKRTRALFMVVIFLVLLMVNAVFGVVIARAFVGTPG
                     AVLPAWTAIVVALIVGQLIRRKFNLMAVSLVGVVALYASVLAGSAMPIELPEIGAMSV
                     QSSWIIILFIYAAIASMLPVWMLLQPRDFINGMQLFVGLILLYGAVFLFMPDISAPAF
                     NDRLPENAPSLVPLLFVTIACGAVSGFHGIVSSGTSSKQLDKETDARFVGYLGAVGEG
                     SLALITIVAVSGAALAATPDLWHQIYDRFGSAGEGAFIEGGANLLNAGWGLPIGIAQT
                     LLATMLVLFAGTTMDTGVRLQRYIIQEWGVIYNIPALNRGVPATLVAVGLCLALAFGA
                     GGGSGSGGLVIWPLFGSTNQILAGMTLLVISVMLIKLGRPARYTLLPMVFVLVISFWA
                     GAVKLLEFFRDGNYLLVVIDAIVLVTSLLVMLEAASVISRFRKGGAVVAEG"
     gene            complement(2530..>3417)
                     /locus_tag="VFY27_08305"
     CDS             complement(2530..>3417)
                     /locus_tag="VFY27_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5765556.1"
                     /translation="TQALLDILIEVPIQSADSRFSVTPELARLGMQTTTVLQFVPPDG
                     NVRAFEFSGNPGLVQLDPRWHQAFFRFVGSGVHHILQGYDHLLFVLCLIIPFRRMRPL
                     VILVTSFTVAHSITLLAAAFGLVPSSLWFPPFIEMLIAASIVYMALENIVGTRWQRRW
                     IIAFGFGLLHGFGFSFVLGGTLQFAGSHLLTSLLAFNLGVELGQLLVILLAVPVLNIA
                     FRAERAERIGTIILSALLAHSGWHWMSDRASAFVQYDVAWLSPGSLGTAVQWLMMLMA
                     AAAVAVLGYRLYRKSSPAL"
BASE COUNT          766 a         1071 c         1036 g          544 t
ORIGIN      
        1 gtcgtccatg tcggcggtgc ccatattctc gaattcttcg ccgctgatga catcaatcgg
       61 cacggacgat tctgtgaccg tccgttccgc ccgccgggtg ccggtagtaa tgatctcttc
      121 gatctcttcg ttgttggcac cttcctgcgc ttgcgcaacc ggtcctatcc ccggcgccac
      181 gagtaatgca gcagaaaggg gcaagaacct aattagctta ggaaccttac gcatctggtg
      241 tctcccccaa tggattagat atctggttat gttcgcgccg cgcttgctca tcaaacaaat
      301 cgggcggccg caacacgtgg ggattgaacc accgtcgtca atgaaaataa agcactggcc
      361 accatttgcg cggctttttt ttagtttcat ctggaatggt ggtggtgaaa tggcgacatc
      421 ggaccaagtg cggcaggctg cgccaccaaa cgagcggggc cccacaatgt gaaactcgat
      481 gtggctttgt tgcggacgta ccacatggtc atccggcgcg cctatccctc cgcgaccacc
      541 gctccgccct tcctgaacct ggatatcacc gaggcagctt cgagcattac cagcaagctc
      601 gtaaccagca cgatcgcgtc tatgacgacg agcaggtagt tgccgtcgcg aaagaactcc
      661 agcaacttga ctgcgcccgc ccagaacgag atgacgagga caaagaccat cggcaaaagc
      721 gtgtagcgcg ccggccggcc gagcttgatc agcatcacgc tgatgacgag cagcgtcatg
      781 ccggcgagga tctggttggt cgagccaaac aggggccaga tcacgaggcc gccgctgccg
      841 gagccgccgc ccgcgccgaa cgccagcgcc aggcagagac cgacagcaac gagggtggcg
      901 gggacgcccc ggttcaaggc cggaatgtta tagatgaccc cccactcctg gatgatgtag
      961 cgctgcaaac gtacacccgt gtccatggtg gtacccgcga acagcaccag catcgttgcg
     1021 agcaaggtct gcgcgatgcc gatcggcaaa ccccacccgg cgttcaacag attggcaccg
     1081 ccctcgatga aggcgccctc gccggccgaa ccgaacctgt cgtagatctg gtgccagaga
     1141 tccggggtcg cggcaagcgc ggcgccgctt acggcgacga tcgtgatcag cgccagcgag
     1201 ccttcgccga cggcgcccag gtacccgacg aagcgggcgt ccgtctcctt gtccaattgc
     1261 ttggaactgg taccggacga gacgatgcca tgaaagccgg atactgcgcc gcatgcgatg
     1321 gtcacgaaca gcaacggcac cagcgacggt gcgttctccg gcaggcgatc gttgaaagcc
     1381 ggcgcggaaa tgtccggcat gaacaggaac acggctccgt agagcaggat cagcccgacg
     1441 aacagctgca tgccgttgat gaagtcacgc ggctgcagca gcatccacac tggcagcatc
     1501 gacgcgatag cggcatagat gaacagtatg atgatccagc tcgactgcac cgacatcgcg
     1561 ccgatctccg ggagttctat cggcatcgca ctgccggcga gtacggatgc gtagagcgca
     1621 actacgccca cgagggagac ggccatcagg ttgaacttgc ggcgaatcaa ctggccgacg
     1681 atcagcgcga ccacgatcgc ggtccacgcc ggcaggactg cgccgggcgt accgacgaag
     1741 gccctggcaa tgaccacgcc gaacacggca ttcaccatca gcagcaccag gaagatcacc
     1801 accatgaaca gcgcgcgcgt gcgtttgccg atgatggctt cggacagcgc gccgatggat
     1861 tttccccggt gccggttgga cgcccacagc gcgccggcat catgcacgcc ggcgaaaaag
     1921 atcgtgccca gcacaaccca cagcattgcc gggacccagc cccagtaaac ggcaatggcc
     1981 gggcctacga tcggagccgc gccggcaacc gaggtaaagt gatggcccca aaggacgtac
     2041 ttgttggtcg gaacgaagtc gacaccgtcg ttgatctggt gcgccggcgt cacgaagtcc
     2101 gggtcgagcc ggaaaatctt cgtggcgatg aatttcgaat aaacgaacca gccgaaggac
     2161 attccgataa tgcccagcac cactatcatg acggactgca tggattcacc cctagtcgta
     2221 cgaccgaacg cctgcgcgcc agcgcgacgc ctgttagctg cgttgtcccc gccgcttgcg
     2281 cgcgagctta acctgaagtg gcgtagacac gaaagcagtc acccgttgaa cgccgtcacg
     2341 acagatcatt cgtcgcgccg gtttcgattg cacttgtagg agcccgccat gcgggcgaat
     2401 tctttaacgc ttatcgaact cgcccgcatg gcgggctcct acaggtgccg gtcgaagccg
     2461 gtccaacgca tcagcgaaaa cgaagacagg cgagccaccg caacggtgcg acgcctgcat
     2521 ggcgggttcc tacagtgccg gggacgattt gcggtacagg cgatagccga gtacggctac
     2581 agcagcagcc gccatgagca tcatcagcca ctggacggcc gtgccaagcg atccaggact
     2641 gagccacgcc acgtcatatt gcacgaaagc cgacgcgcgg tcgctcatcc agtgccagcc
     2701 gctgtgcgcg agcagcgcgg agaggatgat cgtgccgatg cgttcagcgc gttccgcgcg
     2761 aaaagcgatg ttcaggaccg gaacggccag caggataacc agcaactgcc ccagctcgac
     2821 gcccaagttg aatgccagca gcgaagtcag caggtgcgag cctgcgaact gcagcgtgcc
     2881 gcccagcacg aacgagaagc cgaagccgtg caacagcccg aaaccgaaag cgatgatcca
     2941 gcggcgttgc cacctcgtgc cgacgatgtt ctccagcgcc atatatacga tcgaggcggc
     3001 gatcagcatc tcgatgaacg gcgggaacca gagactcgac ggtacaagcc cgaacgccgc
     3061 cgccagcagc gtgatcgagt gcgccaccgt gaacgacgtg accaggatga ccagcgggcg
     3121 catgcggcga aacgggatga tcaggcacag cacgaacagc aggtggtcgt aaccctgcag
     3181 gatatggtgc acaccggagc cgacgaagcg aaaaaacgcc tggtgccagc gcgggtccag
     3241 ctgcaccagg cccggattcc cggagaactc gaacgcgcgg acgttaccat ccggcgggac
     3301 gaactgcagc acggtggtgg tttgcatgcc gagccgcgcc agttcgggag ttacggagaa
     3361 cctggagtca gcggactgta tcggcacctc aatgagtatg tcgagtaacg cctgcgt
//
LOCUS       DASUOM010000398         8506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_380323,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000398 DASUOM010000000
VERSION     DASUOM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380323"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..543)
                     /locus_tag="VFY27_08310"
     CDS             complement(130..543)
                     /locus_tag="VFY27_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX5765557.1"
                     /translation="MTQSFETSSERAIRASPLTAAAAISGLLALSACATAPEPPTQAL
                     QAAESAIVNAEQDRVADYASAELRQAREKLSAARTEVRNEEMVKARYLAEESRVHAEL
                     AAAQAEEIKAKAVNDDMQKSIDTLKQEMKRSSGAR"
     gene            complement(708..1643)
                     /locus_tag="VFY27_08315"
     CDS             complement(708..1643)
                     /locus_tag="VFY27_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5765558.1"
                     /translation="MDPICHTLTGAALGCTGLQKRSRFGRATLIIAANLPDVDALTYL
                     VDGVDSLGIRRGVTHGVPALVVLPVLLALAMKGLSLTLRGSPVKPDADFRQLWLLSAV
                     GMATHPLLDYLNNYGLRWLMPFVDRWVYGDTLFIVDPFVWAILLAGVILTAAVKGDRT
                     AWPHRPAAIALAATGAYIALNAGGTLLARHATVTALQDNPPSRLMASPVLMRPLERRL
                     VLEYEDVYRVGTVDLFPALSVQIEDLAIAKGDPRDLARAAATGEGRVFLHWARFPFSE
                     SESVHNSRVIRVMDARYVREWEDGFGAIEVIVPVD"
     gene            complement(1666..3300)
                     /locus_tag="VFY27_08320"
     CDS             complement(1666..3300)
                     /locus_tag="VFY27_08320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5765559.1"
                     /translation="MILAFILGACESEQQDTATLVVTDARVWTGEPEMPWAEAVASDG
                     EKIVAVGSNADVAGWIGTGTEVISVAGSMLVPGMIDTHVHFVTGGSGLASVQLRDAAT
                     PEEFVARIGAFAEMLEPGEWILNGNWDHENWGGELPRRDWIDAVTPDNPVWVNRLDGH
                     MALANSLALERAGVDADSPDVAGGEIVRDEAGSPTGVLKDNAMGLVDGVVPDPGEAQL
                     DREVAAAMKYVASNGVTSVHDMAGWDSLATYRRAKENGELITRIYSVMPLKDWQRLRD
                     EVATQGRGDQWLQIGGLKGFMDGSLGSHTAAFFEPFTDAPDDRGLLVSDLDDMSSWVS
                     GADAAGLHVMVHAIGDRAIRDLLDIYLDVAEENGERDRRFRIEHAQHIHPDDIERFPV
                     QNVIASMQPYHAIDDGRWAEKVIGPERAETTYAFRSLIESGARVSFGSDWFVAPPTPL
                     EGIYAAVTRRTLDGANPDGWVPQQKITVEEALKAYTSDAAYASFEENIKGTLKPGMLA
                     DFVLLDRDLTAIPAEEIADAKVLKTVVGGKVVFSAD"
     gene            complement(3403..4113)
                     /gene="mtgA"
                     /locus_tag="VFY27_08325"
     CDS             complement(3403..4113)
                     /gene="mtgA"
                     /locus_tag="VFY27_08325"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX5765560.1"
                     /translation="MPRRSEKRGLGTRVLRGAVVLVLLVMLLPVALVLPLRWFPPPVT
                     AFMLQDQSGRVPVLYEWADWPSLGTNAALAVVAAEDQTFADHFGFDVKSIRESVESYN
                     QGDSLRGASTITQQVAKNLYLWPGRSFARKGVEAWFALLIEVFWTKKRILEVYLNIAE
                     FGPGLYGIPAASRIYFNKAATNLSDDEAALLAAVLPNPRRLDASRPSEYVRERQRWIE
                     RHMQRLHREAWITRISQD"
     gene            4365..6269
                     /locus_tag="VFY27_08330"
     CDS             4365..6269
                     /locus_tag="VFY27_08330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5765561.1"
                     /translation="MSRTLRPRFQPRASIALALLVATFLATPAFAQRTVKPVLHGQHW
                     MAITGKPLAATAGAVTFTRGGNAVDAACAMLAATSTMWDTLGWGGETQALIFNPGTGE
                     VKGVNALGVAPTGATAEFFREQGMVYPPEFGPLAAVTPGTPGGLMVMLAEYGRLSLAE
                     VLQPAMRMAEGYPIEKAQADNIERQRDIIAQWPDSKRILLPHWKNDDGQTRAAPFPGE
                     IFSQSDLLATLRKLVDAEAVALKAGKSREQAIMAAYDRFYRGDIARELVRATQAAGGL
                     ITLADLNRWKVYIEEPVVASYRGIDVYKLTSWVQGPVMLQALNILENADLKSMGYNSS
                     RYIHALYQAMNLSFADRDFYYGDPYFPPEEPIEGLLSKQYAKQRYGQIDWSANNESTK
                     PGDPYPFQDGENPFGKLLEQWTPVPPAADALGEKGFQQAMQMSRDEAFLAGTTSIQTA
                     DAEGWVVSVTPSGGWIPPFIAGKTGVGLSQRMQAFVLDERMNPFNVVEPGKRPRATLT
                     PSLALKDGEPYLAFSVQGGDSQDQNLLQFFLNVVEFDMNVQQAAEAANINSYQMQSSF
                     GAHEAEPGRLLVREDVPPWIQDELRKLGYDVETAPRTSGPITAIWFDREHGTMWGGAS
                     DFGEDYGIAW"
     gene            complement(6604..6897)
                     /locus_tag="VFY27_08335"
     CDS             complement(6604..6897)
                     /locus_tag="VFY27_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX5765562.1"
                     /translation="MAERTLSSLRGIVLDEQVTFTLGELSHACGIRTELVVEMVDHGV
                     IEPASSGNEWQFHGNSIVRAQRALRLVRDLELNWPGAALALDLLEELDRLQKR"
     gene            complement(6902..7849)
                     /locus_tag="VFY27_08340"
     CDS             complement(6902..7849)
                     /locus_tag="VFY27_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5765563.1"
                     /translation="MEFKDYYKILGVERDASQDDIKRAYRRLARKYHPDVSKESDAEV
                     RFKEMKEAYEVLKDPEKRSAYDQFGANWKAGQDFQPPPNWQREYSFGGGAESFGDAGQ
                     FSDFFETLFGQARGGGFGGFRTGGRMKGEDVNASVSIPLEDAYRGASRKITLEVPEAD
                     PDGSVRRRRKTLNVKVPAGITAGKRIRLEKQGGPGIGGSPAGDLYLAIDFEPHPFYRA
                     DGRDIHLELPVTPWEAALGRKVKVPTLGGAVDLQIPAGSSSGKMLRLKGRGLPGTPRG
                     DQYVELKVVVPPASDEKTRELYETLERGHSVNPRAKLGV"
     gene            complement(7924..>8506)
                     /locus_tag="VFY27_08345"
     CDS             complement(7924..>8506)
                     /locus_tag="VFY27_08345"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX5765564.1"
                     /translation="LLILVAGHLVLDLGKSAAIRRNPNSDGALLYIADQLLHVALVAV
                     AAAVAVQALPPAAEVLAFWDEVRNTVLVYAVVIVATVFPAGYLIRYLLRPLSQQLAAS
                     RDYQTQHFDSLEGLSNAGLYLGWLERALLVIAFAMGSFTAVGLIIGAKSVARFPEFKS
                     RAFAEYFLIGTLISVAIAAVGGWALRTILLLVH"
BASE COUNT         1642 a         2636 c         2607 g         1621 t
ORIGIN      
        1 gttgcgtcaa cttgttacgc gcggattcag agccttccgg cgccatcaac ggtgtggcac
       61 atgctccgag cacggcgaag gccgccgtag tgacgatgat tttcgcgtac ttgttcctgt
      121 ggctgtacat tatctggctc ctgaactgcg tttcatttcc tgcttcaacg tgtcgatgct
      181 tttctgcatg tcatcgttga ccgccttcgc ctttatctct tccgcttgcg cggcggcgag
      241 ttcagcatgg acccgcgact cttccgcaag gtacctggcc tttaccattt cctcgttacg
      301 cacttccgtg cgagcggcgg acagtttttc acgcgcctgg cgcagttccg cggatgcgta
      361 gtcggcgacg cggtcctgct cggcgttgac gatggccgac tctgcggcct gcagcgcctg
      421 cgtcggcggt tcgggcgcag ttgcgcatgc agacagcgca agcaagccgg aaatggccgc
      481 agcagcggtc aggggtgacg cacgtatcgc cctttcactg gaagtttcga atgactgggt
      541 catgttgatt gtcctggttg gatgacaaac gctggatagc atttgacttc tcacagtatc
      601 agcgccacag aaccagagat ctgtgcggtg gcgtacttag ggctgtttaa ttgcagaacc
      661 ggcagtaaca gcagcatccg gacttcgcgg cggacatgcg ccgagtttca atctacggga
      721 acgatcacct cgattgcgcc gaaaccgtct tcccactccc gcacataccg tgcgtccatg
      781 acgcggatga cccgcgagtt gtggaccgac tccgactcgc tgaacggaaa gcgcgcccag
      841 tgcaggaaaa cgcggccttc gccggtcgct gccgcccggg caaggtcgcg agggtcaccc
      901 ttcgcgattg caagatcttc gatctgtacc gacagcgccg ggaaaagatc aactgtaccc
      961 acgcggtaga cgtcttcgta ttcgagtacc aggcggcgtt ccagaggccg cataaggacc
     1021 ggtgatgcca tgagccggga tggcgggttg tcctgcaatg cagtgactgt cgcgtggcga
     1081 gccagcagcg tgccgccggc gttcaacgcg atatatgcgc cggttgccgc cagcgcgatg
     1141 gccgcagggc ggtgcggcca agccgtccgg tcgcctttca ccgctgctgt aaggatgact
     1201 ccggccagca gtattgccca cacaaaagga tcaacgatga acaacgtgtc gccgtacacc
     1261 cagcgatcca cgaacggcat tagccagcgc aggccgtaat tgttgagata gtcgagtaaa
     1321 ggatgagtag ccatgccgac cgctgacaag agccacagtt ggcggaagtc tgcatccggc
     1381 tttaccggag agccacgcag ggtcaaggaa agccccttca tggccagcgc cagcagtacc
     1441 ggcaacacga ccagcgcggg gacgccgtga gtcacaccgc gccgaatgcc gagggagtcg
     1501 acaccgtcga cgaggtaagt cagggcgtcg acatcgggga gattggcggc gattatgagc
     1561 gtggcgcggc cgaagcggga gcgcttctgc aaaccggtgc agccaagcgc cgcaccggtc
     1621 agcgtgtggc agatcgggtc caccgttgct gggctgccgg ttgcctcagt cggccgagaa
     1681 aaccaccttg ccgccgacca ccgtcttcaa taccttggca tccgcgattt cttccgcagg
     1741 tattgccgtt aagtcccggt cgagcagcac gaagtcggca agcatgcccg gcttcagcgt
     1801 gcctttgatg ttctcctcga aagatgcgta cgcggcatcg gaggtgtatg ccttgagcgc
     1861 ctcttcgacc gtgatcttct gctgcggtac ccagccgtcc gggttcgcgc cgtccagcgt
     1921 gcgccgggtg acagccgcat agatgccttc cagcggtgtc ggcggtgcga cgaaccagtc
     1981 gctgccgaag gatacgcgcg cgccgctctc gatcagcgac cggaaggcgt aggtcgtttc
     2041 ggcgcgttcc gggccgatga ccttctccgc ccagcgcccg tcgtcgatcg cgtgatacgg
     2101 ttgcatgctg gcgatgacgt tctgcacggg gaaacgttcg atatcgtccg ggtggatgtg
     2161 ttgcgcatgc tcgatgcgga agcggcgatc acgctcgccg ttctcttccg cgacgtccag
     2221 gtagatatcc agcaggtcgc ggatggcgcg atcgccgatc gcgtgcacca tcacgtgcaa
     2281 gccggcggcg tcggcgccgc tgacccatga gctcatatcg tcgaggtcac tcacgagcag
     2341 gccgcgatcg tccggcgcat cggtgaatgg ctcgaagaag gcggcggtat gcgagccgag
     2401 cgagccgtcc atgaatccct tcaatccgcc gatctgcagc cattggtccc cgcgtccctg
     2461 cgtcgccacc tcgtcccgga gacgctgcca gtccttaagc ggcatcaccg agtagatgcg
     2521 ggtgatgagt tcaccgtttt ccttcgcgcg ccggtacgtt gcgaggctgt cccacccggc
     2581 catgtcgtga accgaggtga cgccgttcga agcaacgtat ttcattgccg ccgcaacttc
     2641 ccgatcgagc tgcgcttcgc cggggtcggg cactacgccg tcgaccaggc ccatcgcgtt
     2701 gtccttcagg acgccggtcg gtgacccggc ctcgtcccgc acaatttccc cgcctgcgac
     2761 atccggcgaa tccgcatcaa caccggctct ctccagcgcg agcgagttcg ccagcgccat
     2821 gtggccgtcc agccggttga cccaaacggg attgtccggc gtgaccgcat caatccagtc
     2881 gcgccgcggc agttcgccgc cccagttttc atggtcccag ttcccgttca gtatccattc
     2941 gcccggctcg agcatttcgg cgaaggcgcc aatccgtgcg acgaattcct cgggcgttgc
     3001 ggcatcccgg agctgcaccg atgcaagacc cgagccgccg gtaacgaagt gcacgtgcgt
     3061 gtcgatcatt cccgggacga gcatgctgcc ggcgaccgat atgacttccg tacccgtgcc
     3121 gatccatccg gcgacatcgg cattgctgcc gaccgcgaca attttttcgc cgtcgctcgc
     3181 cacagcttcc gcccatggca tttccggctc gccggtccat actctcgcgt cggtgaccac
     3241 cagggtcgcc gtgtcctgct gttccgattc acaggcgccg agaataaaag cgagaatcaa
     3301 taaagtgcag ctacgaaaag tatcggacat gctgatcccc atttgtttag tcgccgcatt
     3361 gtaagcgatg atgcccgtga gcgccggcag tgcaagttgc agtcagtcct gggatatgcg
     3421 ggtaatccag gcttcgcgat gcaggcgttg catgtgacgc tcgatccagc gctggcgctc
     3481 ccgcacatat tcggacggcc gtgaggcatc gaggcgtcgc ggattcggca gcacggccgc
     3541 cagcagcgcc gcctcgtcgt cgctcaaatt cgtggccgcc ttgttgaagt agatgcggct
     3601 ggcggcggga ataccgtaaa gtccgggacc gaactccgct atgttcagat aaacctccag
     3661 gatccgtttc ttcgtccaga agacctcgat caacagcgcg aaccatgcct cgacgccttt
     3721 cctggcgaaa cttcgcccgg gccacaggta caggttcttc gctacttgct gcgtaatggt
     3781 cgatgcaccg cgcaaggaat cgccctggtt gtagctctcg acagattcgc ggatcgactt
     3841 cacgtcgaat ccgaagtgat cggcaaaggt ctggtcctcc gcggccacga cagcgagggc
     3901 cgcattagtc ccaagtgacg gccagtcggc ccactcgtaa aggaccggca cgcggccgct
     3961 ctggtcctgc agcatgaacg cggtcaccgg cgggggaaac catcgcagcg gaagcaccag
     4021 cgcgacaggc aaaagcataa cgagcaaaac gagtacgaca gcgcccctga gcacgcgcgt
     4081 gccaagaccg cgcttttcgc ttcttctcgg cacgtcaggt gaaacctcta cgcatcacat
     4141 gcgaggatga cgcaggccta cctggagcgt agttcgtctg acattgactg atcctgcgcg
     4201 acgcggccta tatccatggc catacctatg gccgatgttc ggctgatacc atagggtacc
     4261 gaatctataa tatgcgtgcc gccgcgctcg ccgtacgcac tgtacccgta cacggcatgt
     4321 gctcgagcgg acctagctct tcagacccga tacaggtgac gacaatgtca aggacactgc
     4381 ggccgcgctt tcagccccgc gcaagcatcg ccctcgcgct cctcgtggcg acgtttctcg
     4441 cgacacccgc cttcgcacag cgcacggtaa agcccgtgct ccacgggcag cactggatgg
     4501 caatcaccgg caagccgctc gccgccaccg cgggagcggt gacgttcacg cgcgggggca
     4561 acgccgtcga tgcggcctgc gcgatgctgg cggctacatc gacgatgtgg gacacgctcg
     4621 gctggggagg cgaaactcag gcactcattt tcaatccagg gacgggcgag gtgaaaggcg
     4681 tgaacgcact cggtgtcgca ccgaccggag cgaccgctga attctttcgc gagcagggca
     4741 tggtgtatcc gccggagttc ggaccgctcg ccgccgtcac acccggtacg ccgggcggcc
     4801 tgatggtcat gctcgccgag tacgggcgcc tgagtcttgc cgaggtactg caaccggcga
     4861 tgcggatggc ggaaggttac ccgatcgaga aagcgcaggc cgacaatatc gagcgccagc
     4921 gcgacatcat cgcgcagtgg ccggactcga agcgcatcct gctgcctcac tggaagaacg
     4981 acgacgggca aacccgggct gcgccgtttc ccggcgaaat attctcacag tccgatttgc
     5041 tggcgacgct gcgaaagctc gttgacgccg aagcggtcgc attgaaagcg ggaaagagcc
     5101 gcgagcaggc gatcatggcc gcttacgacc gtttctaccg cggcgacatc gcaagggagc
     5161 tggtgcgcgc aacgcaggca gcgggaggcc tgatcacgct cgcagacctc aaccgatgga
     5221 aggtctacat cgaagaaccg gtcgtggcca gctatcgcgg catcgacgtt tacaaactga
     5281 ccagctgggt ccagggcccg gtaatgctgc aggcgctgaa catcctcgag aatgccgacc
     5341 tcaagtccat gggttacaac agcagccgct acattcacgc gctctaccag gctatgaatc
     5401 tctcgttcgc cgaccgcgac ttctactacg gcgacccgta cttcccgccc gaggagccga
     5461 ttgaaggctt gctgtcgaag cagtacgcaa agcagcgata cgggcagatc gactggtcgg
     5521 cgaacaacga gagcacgaaa cccggagacc catacccgtt ccaggacggc gagaatccct
     5581 tcgggaaact gctggagcaa tggacaccgg tacccccggc tgccgatgca ctgggggaaa
     5641 agggttttca gcaggccatg cagatgagca gggacgaggc ttttcttgcg gggaccacat
     5701 cgatccagac ggcggacgcc gaaggttggg tggtttcggt tacgccgagt ggcggctgga
     5761 ttccgccgtt catcgcgggc aagaccggcg tcggcttgag ccagcgcatg caggcattcg
     5821 tgctggatga gcgcatgaat cctttcaacg tggtcgagcc ggggaagcgc ccgcgcgcaa
     5881 cactgacgcc gagtctcgcg ctcaaggacg gcgaaccata cctcgcgttc tcggtgcagg
     5941 gaggcgattc gcaggaccag aacctgctgc agttcttcct taacgtggtc gagttcgata
     6001 tgaacgtgca gcaggcggcc gaagccgcga acatcaacag ctaccagatg cagagttctt
     6061 tcggggcgca cgaagcggag ccgggccggc tactggtgcg tgaggatgtg ccgccgtgga
     6121 tccaggatga gttgcggaaa ctgggttacg atgtcgagac ggcgccgcga acgtctggtc
     6181 cgatcacagc gatctggttc gatcgtgaac acggaaccat gtggggaggt gccagcgact
     6241 tcggcgagga ctacggcatc gcctggtaaa cagcgcgagc acgagctgct gcaacagcat
     6301 catccaagtt aaagagagcg gtgagtacac ggcgttcgtc tggaaccgtg ctttttccgg
     6361 aaaccagttt ttgagcacag atgtatggca tcttccgcga tttgatgtat aaagtacctg
     6421 ctcgcgcact gcaggagtcc gttggagata gagtaagcaa tttttttaac ggtacccaag
     6481 ctatcaacac gggctcctgc agttgccatt ttgcttctgc cagaaaaggc aagttgaaaa
     6541 aagtttgttt cgaaaagacc cggcgaaggg tctttttttt cagcctcgat cgccggtgcg
     6601 gagctatcgt ttctgcaagc gatccagttc ctccagcaaa tccagtgcga gcgcggcgcc
     6661 cggccagttg agttcgaggt cgcggacgag gcgcagggca cgctgcgccc ttacgatcga
     6721 gttgccgtga aactgccact cgttgccgga cgatgcaggt tcgatgacac cgtggtccac
     6781 catctcgaca accagttctg tccgtatccc acaggcgtgg ctgagttccc cgagagtaaa
     6841 cgtaacctgc tcatcgagga cgataccccg cagactgctt aaagttcgtt ccgccatggg
     6901 atcacactcc gagtttggcg cgtgggttca cgctgtggcc gcgctccagg gtttcatata
     6961 actcccgcgt tttttcatcg ctggccggtg gtaccacgac cttcagttcg acgtactggt
     7021 cgccgcgcgg cgttcccgga aggccccggc ctttcagccg caacattttt ccggaactgg
     7081 aacccgcggg tatctgcaaa tccaccgcgc ctcccagggt gggcactttc acctttcgcc
     7141 cgagcgcggc ctcccagggt gtaaccggca attccaggtg aatgtcgcgg ccgtcggcgc
     7201 gatagaacgg gtgaggctcg aagtcgatgg cgaggtacag gtcgccggca ggcgaaccgc
     7261 cgataccggg tccgccctgt ttctcgagcc ggatgcgctt gccggcggtg atgcccgccg
     7321 gaaccttcac gttcagggtc ttgcgccgcc gtcggacact gccgtcaggg tcggcttcgg
     7381 gaacttcgag cgtgatcttg cgtgaagccc cacgataggc atcttcgagg ggaatgctga
     7441 cgctggcatt gacgtcttcg cctttcatcc gccctccggt cctgaaaccg ccgaagccgc
     7501 cgccgcgtgc ctggccgaac aacgtttcga agaagtcgct gaactgtccg gcgtcaccga
     7561 aggattcggc accgccgcca aaggaatact cccgctgcca gttcggtggc ggctggaaat
     7621 cctggcccgc cttccagttg gcgccgaact ggtcgtatgc cgaacgcttc tccggatcct
     7681 tcaggacctc ataggcttcc ttcatttcct tgaagcgcac ttccgcatcc gactctttgc
     7741 tgacgtcggg atggtatttg cgcgccaggc gccggtaagc gcgtttgata tcgtcctgcg
     7801 acgcatcgcg ctcgacgcca agtattttgt agtagtcctt gaattccatg ctgcggttgc
     7861 tccggatttt cgccgcttca aatgtaggca acgggtcagg atttcaacgc ccagtgtgtg
     7921 cgcctagtgt acgagaagca gaatggtgcg cagggcccag cctcccacgg cggcgatcgc
     7981 gacgctgatc aaagtaccga tgaggaagta ttcggcgaaa gccctcgact tgaattcggg
     8041 aaagcgcgcg acggacttgg cgccgatgat caaccctacc gctgtgaagg aacccatggc
     8101 aaacgcaatc acgagcaggg ctcgctcgag ccatcccagg taaagccccg cgttgctcaa
     8161 accctcgaga gaatcgaagt gctgcgtctg gtaatcgcgc gaggcagcaa gctgctggct
     8221 cagcggccgc agcaggtagc gaatgaggta gcctgcggga aagacggtcg caacgatgac
     8281 gacggcgtat accaggaccg tgttccgcac ctcgtcccag aacgcgagca cttcggctgc
     8341 cggcggcaag gcctgaaccg cgacggccgc cgcgaccgca acgagggcta catgcagcag
     8401 ctggtccgcg atgtagagca gcgcgccgtc tgagttcggg ttcctgcgta tggcggctga
     8461 tttgccgaga tcgagcacca ggtgcccggc gaccaggatc aggaga
//
LOCUS       DASUOM010000399         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_84725,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000399 DASUOM010000000
VERSION     DASUOM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84725"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFY27_08350"
     CDS             complement(<1..273)
                     /locus_tag="VFY27_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5765565.1"
                     /translation="MSTESKVIAVGEFFELEPGADVLASSRYNADIAPTKIAERTWNQ
                     WHVAALWVGMSICVPTYTLGGILTAYFGLSVAEALWTILLANIVILI"
     gene            complement(333..1205)
                     /locus_tag="VFY27_08355"
     CDS             complement(333..1205)
                     /locus_tag="VFY27_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5765566.1"
                     /translation="MPKLKCGLIQMNFKADVSMHPDRIRDIMIEAHLPYIEEAGQKGV
                     QVLCFQEVFTQPYFCPSQDRKWYAAAERIPDGHTTRLMQEHAKKHSMVIVVPIYEEAM
                     PGVYYNTCAVIDADGSYLGKYRKTHIPQVDPGFYEKFFFKPGDLGYPVFDTAYVKLGV
                     YICYDRHFPEGWRALALNGAEYIVNPSATVAGLSKYLWELEQPASAAANGVFIGAINR
                     VGREQPWADRMGEFYGSSYIVNPRGTIEAQASYGDDELLVHEIDLEMIREVRNTWQFF
                     RDRRPELYGPLVGG"
     gene            complement(1219..2037)
                     /locus_tag="VFY27_08360"
     CDS             complement(1219..2037)
                     /locus_tag="VFY27_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEX5765567.1"
                     /translation="MYQKRSAYRLLSMFMALSATGCAQQQLVPPTDEEPDLGILWVRD
                     SAEYRALSLQAYGAAAEDLSGLLEDKTWSALPHQADAHDLPPAIIFDVDETLVTNVVF
                     QAELEPPFSNHKLDDWNAAGTATPVPGAPEFVKLARAAGVDVFFITNRPCERKEGIAD
                     PCPQKAVTVDDLNEAGIPAGASDVLMANERPEWTREKKSRRDVIAQDYRVIMLVGDDL
                     GDFIPCTRARPVAPCTEGATKASRDRDVDEHQAYWGNGWYVLPNPMHGSWTTVR"
     gene            complement(2166..>5285)
                     /locus_tag="VFY27_08365"
     CDS             complement(2166..>5285)
                     /locus_tag="VFY27_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765568.1"
                     /translation="TDTRTGNSRTATTSDTGQFSLSGLRIGGPYTIQVEQAGMAPQTI
                     TDVELDLGDTYTFSVALSPEEMEEITVTAQALASVQVALGPSATFSLADLEDAPAINR
                     NINDVISIDPRIYVDEAFNDAVQCGGASARFNSLTVDGVRMNDNFGLNSNGYPTERMP
                     FPYDAIEQVAVELAPFDVNYGGFTACNINAVTKSGTNEFHGSVFYDYTDDSLTGDTLE
                     GDSLSLGTFEEKRYGLTFGGPILEDKLFFFAAYEKLEGANLFSRGPADSSSAVPVLGV
                     TQAQLDRIRQVAQDVYGYDVGPTPSSLPNEDEKYLIKLDWNINDDHRASYTYTFNDGF
                     NINESDDADDEFEFANHYYERGAELNSHVAQLFSSWGQNFTTEMRISYAELDNRQISL
                     GEPGFGEVQIATFNDPDGAGPISAQQATVYIGGDDSRQSNKLYYENNSYKLAGTYQAG
                     DHVITGGWEREEFDVFNLFIQHTEGEFRFDGFDQCSGADPDGCIDDFETGDPDDVFYG
                     NAAPSNNPDDGSASFAYEINSLYLQDEFPVADGAVTLVAGVRYDWYTSSDDPRENPLF
                     FARNGFANTTTFDGEGLIQPRLGFNWDVSDTLSVRGGAGLYSGGNPNVWLSNNYSNDG
                     QTVVQVSMFDLQASPDSLFTIPHAGGGNPLFDIPQQLVDGVTTGTANSGVNALDPNFD
                     IPKEWKFALGATWDFDAGFFGSGYRLMADYLHTEKEDSALIIDGTLTQIGTAPDGRPL
                     YRSIDGSDPDCAVDPTSPDCSSRAFNQDFILTNVPGNDGDSDVWSVSLSKSYDWGLDW
                     TFGYAYTESSEVSPMTSSVAFSNFANISVSDPNDPGVTRSNYNTPNRFTFRANYRHAF
                     FGDYLTKFTLFGRVNEGVPFSAVYLNGGDLFGDTLDFRHLIYVPTGPDDPNVVFDPGF
                     DQAAFFAYLEDTGLNEYSGEIAPRNAMTSSWWHKYDVKIEQELPGFNPEHRFSAFLLV
                     ENIGNLINDDWGVLKEQGFPRNQAVIQLDEGTGTGPYVYQNFFTPAPQGRVADASLWE
                     IRFGVSYDF"
BASE COUNT          921 a         1554 c         1712 g         1098 t
ORIGIN      
        1 aatgaggatg acgatgttgg caagcaggat ggtccacagc gcttcggcaa ctgaaagacc
       61 gaaataggcg gtgagaatgc cgccgagcgt gtaagtgggg acgcagatgg acatcccgac
      121 ccagagcgcc gccacgtgcc actggttcca ggtacgttcg gcgatcttcg tcggcgcgat
      181 gtcggcgttg tagcgggagc tcgcgagaac atcggcgccg ggttcgagct cgaagaactc
      241 gccgacggcg atgaccttgc tttcggttga cagtgttact gcgtctcccc cggtctgccc
      301 gcacaggttc tcatgttgtt ggcgcactcg ccttagccgc ccaccagcgg accgtacagc
      361 tccgggcgcc ggtcgcggaa gaactgccag gtgttgcgca cctcgcggat catctccagg
      421 tcgatctcgt ggaccagcag ttcgtcgtcc ccgtagctcg cctgcgcctc gatagtgccg
      481 cgcgggttca cgatgtagct cgagccgtag aactcgccca tcctgtccgc ccacggttgc
      541 tccctgccaa ccctgttgat cgcgccgatg aagacgccgt tcgcggcggc ggaagccggc
      601 tgctccagtt cccacaggta cttgctcagg ccggcgacgg tggccgacgg gttcacgatg
      661 tactcggcgc cgttcagcgc gagcgcgcgc cagccctccg ggaagtggcg gtcgtagcag
      721 atatagacgc cgagcttgac gtacgccgta tcgaacaccg ggtagccgag gtcgccgggc
      781 ttgaagaaga atttctcgta gaagccgggg tcgacctgcg ggatatgggt tttgcgatac
      841 ttgccgaggt agctgccatc cgcatcgatt accgcgcagg tgttgtagta gacgcctggc
      901 atggcctctt cgtaaatcgg cacgacgatc accatgctgt gcttcttcgc gtgctcctgc
      961 atgagccggg tggtgtggcc gtcggggatt ctttcggcgg cggcgtacca cttccggtcc
     1021 tggctagggc agaagtaggg ctgcgtgaag acttcctgga agcaaagcac ctgcacgcct
     1081 ttctggcccg cttcctcgat gtagggcaga tgcgcttcga tcatgatgtc gcggatccgg
     1141 tccgggtgca tcgagacgtc ggccttgaag ttcatctgga tcaggccgca ttttagtttt
     1201 ggcatggtgg tcctcgcttt atcgtaccgt tgtccatgag ccgtgcatcg ggttggggag
     1261 cacgtaccag ccgttccccc agtatgcctg gtgttcgtcg acgtcccggt cgcggctcgc
     1321 cttggttgcc ccttccgtgc agggtgcgac cggccgcgcg cgggtgcagg ggatgaagtc
     1381 gcccaggtcg tcgccgacca gcatgatcac ccggtagtcc tgcgcgataa cgtcgcggcg
     1441 cgattttttt tcccgcgtcc actccggcct ttcgttcgcc atcagcacgt cagaggcgcc
     1501 ggcgggtatt cccgcttcgt tcaggtcgtc caccgtcacg gctttttgcg gacagggatc
     1561 ggcgattcct tccttgcgtt cgcaggggcg gttggtaatg aaaaagacat caacgccggc
     1621 cgcgcgcgcc agcttcacga actccggcgc ccccggcacc ggcgtcgccg tgcccgcggc
     1681 gttccagtcg tcgagcttgt gattgctgaa tggcggttcc agttcggcct ggaacacgac
     1741 gttggttacc agcgtttcgt ccacgtcgaa gatgatcgct ggcggcaggt cgtgcgcgtc
     1801 ggcctggtgc ggcagcgcgc tccaggtttt gtcttcgagg aggccgctta aatcctcggc
     1861 tgcggcccca taggcctgca gcgaaagtgc gcggtattcg gcagagtcgc gcacccagag
     1921 gatgccgagg tcgggttcct cgtcagtggg tgggaccagc tgttgttgtg cgcatccggt
     1981 agcggatagt gccatgaaca ttgacagaag ccgatacgcc gatcttttct ggtacatccc
     2041 ccgcgaccct gactactgct ttgccaaaga gtaccacgcg gtgggaagcg gttgcgtgcg
     2101 gtcgcccgtg agagaaaaaa agggcggccc tgaaaggccg cccctccgtt tcttgttgtt
     2161 agcgatcaga agtcgtagga aacaccgaag cgaatctccc acagcgatgc atccgcgacc
     2221 cgtccctgcg gtgccggtgt gaagaaattc tggtacacgt acggcccggt tcccgtgccc
     2281 tcatcgagtt gtatcaccgc ctggttgcgc gggaatccct gctccttcag cacgccccag
     2341 tcatcgttga tcaggttgcc gatgttttcg accaggagga acgcggaaaa acggtgctca
     2401 ggattgaagc ccggcagttc ctgctcgatc ttgacgtcgt acttgtgcca ccaggaactt
     2461 gtcatcgcgt tacgcggtgc gatctctccg gagtactcgt tcagtcccgt gtcttccagg
     2521 taggcgaaaa atgccgcctg gtcgaaaccc gggtcgaaga ccacgtttgg atcgtccggg
     2581 cccgtcggca cgtagatcag gtggcggaag tcgagggtgt cgccgaacaa gtcgccgccg
     2641 ttcaggtaga ccgcgctgaa cggcacgcct tcgttcaccc ggccgaagag ggtgaacttg
     2701 gtcaggtagt cgccgaagaa cgcatgccgg taattggcgc ggaacgtaaa gcggttcggg
     2761 gtgttgtaat tcgaacgcgt aacgcccgga tcgttgggat cggataccga gatgttcgcg
     2821 aagttcgaga acgccacaga gctggtcatg gggcttactt ccgacgactc cgtgtaagcg
     2881 tagccgaacg tccagtcgag gccccagtca taggacttgc tcagcgacac ggaccagaca
     2941 tcagagtcac cgtcgttacc gggcacgttc gtcaggatga agtcctggtt gaaggcgcgg
     3001 ctcgaacagt caggagaggt cggatcgacg gcacagtccg gatcgctgcc gtcgatgctg
     3061 cggtacagcg gccgcccatc tggcgcggtg ccgatttgcg taagcgtgcc atcgatgatc
     3121 agcgccgagt cttccttctc ggtgtgcagg tagtcggcca tcagccggta gccgctgccg
     3181 aagaacccgg catcgaaatc ccaggtggcc cccagcgcga atttccactc cttgggaata
     3241 tcgaaattgg gatcgagcgc attcacgccg ctgttcgcgg tgccggtggt aacgccatcg
     3301 accaactgtt gcggtatgtc gaacagggga ttaccgccgc cagcgtgcgg gatcgtaaag
     3361 aggctgtccg gtgacgcctg caggtcgaac atgctgacct gcaccacggt ctggccgtcg
     3421 ttcgagtagt tgttcgaaag ccagacgttg gggttgccgc cggaatagag acctgcgccg
     3481 ccgcgcacgc tcagggtgtc ggaaacatcc cagttgaagc cgaggcgagg ctggatcagc
     3541 ccttccccgt caaaggtggt ggtgttggcg aagccgttac gggcgaagaa cagcgggttt
     3601 tcccgtgggt catcgctgct ggtgtaccag tcgtaacgca cacctgcaac gagggtcacg
     3661 gcgccgtcgg ctaccggaaa ctcgtcctgc aggtacaggg aattgatctc gtaggcgaac
     3721 gaagcgctgc cgtcgtccgg attgttgctc ggcgcagcgt tgccatagaa gacgtcgtcc
     3781 gggtctccgg tttcgaaatc gtctatgcag ccgtcggggt cggcgccaga acactggtcg
     3841 aagccgtcga agcgaaactc gccttcggtg tgctggatga agaggttgaa tacgtcgaac
     3901 tcctcgcgct cccaaccgcc ggtgatcacg tggtcgccgg cctggtaggt gccggcgagc
     3961 ttgtagctgt tgttctcgta atagagcttg ttggactgcc gggaatcgtc gccgccgatg
     4021 tatacggttg cctgctgcgc gctgattggt ccggcgccgt ccgggtcatt gaacgtcgcg
     4081 atctgcactt cgccgaagcc cggttcgccg agcgaaatct ggcggttatc gagttccgcg
     4141 taggatatgc gcatttcggt cgtgaagttt tgcccccagc tggagaacaa ctgggcaacg
     4201 tgcgagttca gttcggcgcc gcgctcgtag tagtggttgg caaactcgaa ctcgtcatct
     4261 gcatcgtccg actcgttgat gttgaagccg tcattgaagg tatacgtata ggacgcgcgg
     4321 tgatcgtcgt tgatgttcca gtcgagcttg atgaggtatt tctcgtcctc gttcggcagg
     4381 ctggagggcg tggggccgac atcgtaaccg tacacgtcct gggcgacctg gcggatccgg
     4441 tcgagctgcg cctgggtcac gccgagcacc ggcaccgcgg agctggaatc ggcgggaccg
     4501 cgggagaaca ggttcgcgcc ttccagtttt tcgtaggcgg cgaaaaagaa cagcttgtcc
     4561 tcgaggatcg ggccaccgaa cgtcaggccg tagcgcttct cctcgaacgt cccgaggctg
     4621 aggctgtcgc cctccagggt gtcgcccgtg agcgagtcgt cggtgtagtc gtagaacacc
     4681 gacccgtgaa attcgttggt gccggacttg gtcacggcgt tgatgttgca ggccgtgaat
     4741 ccgccatagt tgacatcgaa cggtgcaagt tcgactgcaa cctgctcaat ggcatcgtac
     4801 gggaaaggca tgcgctccgt cggatagccg ttgctgttca ggccgaaatt gtcgttcatg
     4861 cggacgccgt cgaccgtcag cgagttgaaa cgggcgctgg cgcctccgca ctgcaccgcg
     4921 tcattgaacg cctcgtcgac gtagatgcgc gggtcgatgg agatgacgtc gttgatgttc
     4981 cggttgatcg ccggcgcatc ctccagatcc gccagcgaga acgtggcaga aggcccgagc
     5041 gcaacctgca cggaggccag cgcctgtgcg gttacggtga tttcttccat ctcctcgggg
     5101 ctcagcgcga cactgaaggt gtaggtgtcg ccaagatcga gctcgacgtc cgtgatcgtc
     5161 tgcggggcca tgccggcttg ctcgacctgg atcgtatatg gtccgccgat acgcagaccg
     5221 ctcagcgaga actgccccgt atcggaggtc gtcgcggtcc ggctgttgcc cgtgcgcgtg
     5281 tcggt
//
LOCUS       DASUOM010000400         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1264202,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000400 DASUOM010000000
VERSION     DASUOM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264202"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="VFY27_08370"
     CDS             <1..1623
                     /locus_tag="VFY27_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765569.1"
                     /translation="ERFALAMRLNNLLAAPGFEAWMHGEVLDPARLFFTESGKPRISI
                     MSIAHLPESERMFFVSMLLNELVSWMRAQQGTTSLRAILYMDEIFGYMPPVANPPSKQ
                     LFLTLLKQARAYGLGLVLATQNPVDLDYKGLSNTGTWFIGRLQTERDKARVMEGLEGA
                     AHGGTFDRPAMERTLAGLGRRQFLLHNVHEDEPVVFSTRWVMSYLAGPMTRDQVRSLM
                     WGTDNVPAAAAAKPAVPAGTSPAGGDAPLLPPAIRQFHLPSSQAKPVYRPRLAGAADI
                     GISSSRHGVEETRQCFYALDLEDGPVPVDWTRAEKLSLAVDDVLQQAPPGAMFIPCPA
                     PLSNAANYARWERQFKTWLRQNECIPLLKSPRFRLTSEPGETEGEFRARLQQTAAERR
                     DEAIEKVRKRYAGKASTLENRLLRARQAIEREASQSNSKMVSTAVSFGTAILGAVLGR
                     KRLSATSASRVGAAIRSASGARKQASDVERARQTEAQVMADIEQLNAELDAEIDGLDT
                     TCDAQSEELSEIIVRPRAADIHVSLFGLLWTP"
     gene            complement(1639..>2130)
                     /locus_tag="VFY27_08375"
     CDS             complement(1639..>2130)
                     /locus_tag="VFY27_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HEX5765570.1"
                     /translation="VCAIDGCPDVFEPVCGMNARTYRNRCEAELERIPVQRQGACDVA
                     NCPEVYVPVCGSDNQTYDNACKAEGAGLQSFTLGACDARPCPGMYVPVCGADARTYGN
                     ACQAERNGVRILHAGLCESQGKNCPRVYQPVCGIDGNTYENQCQLENAGVTLAYAGAC
                     LGQ"
BASE COUNT          393 a          696 c          694 g          347 t
ORIGIN      
        1 gagcgcttcg ccctggcgat gaggctcaac aacctgcttg cggcgccagg tttcgaggcg
       61 tggatgcacg gcgaagtcct ggaccccgcg aggctcttct tcacggaatc cggcaagccg
      121 cgaatctcga tcatgtcgat cgcccacctg ccggagtcgg agcgcatgtt cttcgtctcc
      181 atgctgctga acgaactggt cagctggatg cgcgcgcagc agggaacgac cagcctccgc
      241 gccatcctct acatggacga gatattcggc tacatgccgc ccgtcgccaa cccgccgagc
      301 aagcagctgt tcctgaccct tctcaagcag gcgcgcgcct acggcctggg gctggtgctg
      361 gcgacgcaaa accccgtcga cctggattac aagggactgt ccaacaccgg cacctggttc
      421 atcgggcgcc tgcagaccga acgggacaag gcgcgggtga tggaggggct cgaaggcgcg
      481 gcacacgggg gcacgttcga caggccggcg atggagcgaa ccctcgcggg cctcggcagg
      541 cgccagtttc tcctgcacaa cgtgcatgaa gacgagccgg tggtcttcag cacgcgctgg
      601 gtgatgtcgt accttgccgg cccgatgacg agggaccagg tgcgctcgct catgtggggc
      661 acggataacg tacccgcagc cgccgccgcg aagcccgctg tgccggcagg gaccagcccg
      721 gccggcggcg acgcgccgtt gttgcctccg gcgatccggc aattccacct gccttcgtcg
      781 caggcaaagc ccgtgtacag gccgcgcctg gccggcgccg ccgatatcgg aatctcgtcg
      841 agccggcacg gcgtcgagga gacccggcaa tgtttctacg cgctggatct tgaagacggt
      901 cccgtccccg tcgactggac gagagctgaa aagttatcgc tggcagtcga cgacgttctg
      961 cagcaggcgc cgccgggcgc gatgttcatt ccctgccccg cacctctttc gaacgccgcc
     1021 aactacgcgc gctgggagcg gcagttcaag acctggctgc gacaaaacga atgcatcccg
     1081 ttgctgaaga gccccaggtt ccggctgact tcggaacctg gcgaaaccga gggtgagttc
     1141 cgcgcgcgat tgcagcagac cgcggcggag cggcgcgacg aagctatcga gaaagtgcgg
     1201 aagcgttatg ccggcaaggc gagcacgctc gagaacaggc tgttgcgcgc ccggcaggcg
     1261 atcgaacgcg aggcctcgca gtcgaactcg aagatggtga gtacggcggt atcgttcggc
     1321 acggcaattc ttggcgccgt cctgggacgc aaacgcctgt ccgccacttc cgcaagtcgt
     1381 gtgggtgcgg ccatccggag cgcgagcggc gcgaggaagc aggcatcaga cgtcgagagg
     1441 gcgaggcaga cggaggcgca ggtgatggcg gacatcgagc aactcaatgc ggagcttgat
     1501 gcggaaatcg acggcctcga tacaacctgc gacgcacagt cggaggaact gtcggagatc
     1561 atcgtcaggc ccagggccgc cgatatccac gtttcgctat ttgggctgct ctggactccc
     1621 tgagggaccc gccggcgcct actggccgag acaggcacct gcataggcca acgtgacgcc
     1681 ggcattttcc agctggcact gattttcata tgtattgcca tcgatgccgc agaccggctg
     1741 gtagacgcgc gggcagttct tgccctgcga ctcgcaaagc ccggcatgca ggatgcgcac
     1801 gccgtttcgc tccgcctggc aggcattgcc gtaggtcctg gcatccgccc cgcaaaccgg
     1861 cacatacatg cccggacagg gcctggcgtc gcacgcgccc agggtgaagc tttgcagtcc
     1921 cgcgccctcg gccttgcacg cattgtcgta ggtctggtta tcggaaccgc acaccggcac
     1981 gtagacttcc gggcaattcg caacgtcgca ggcaccctgc cgctgcacgg ggatacgctc
     2041 cagttccgct tcgcagcgat tgcgataggt gcgcgcgttc atgccgcaca ccggctcgaa
     2101 aacgtcaggg caaccatcga tcgcgcacac
//
LOCUS       DASUOM010000401         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_867401,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000401 DASUOM010000000
VERSION     DASUOM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867401"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFY27_08380"
     CDS             complement(<1..284)
                     /locus_tag="VFY27_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765571.1"
                     /translation="MTVTLFLACLVQPVLAAEQSEVPQLSPSQHSGSALARELHEWAV
                     KKEKMDLYLRDAMRRQSIDMWIILSREFNPDPILDLFGGLGISGWYGHRNA"
     gene            complement(368..1366)
                     /gene="ispB"
                     /locus_tag="VFY27_08385"
     CDS             complement(368..1366)
                     /gene="ispB"
                     /locus_tag="VFY27_08385"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEX5765572.1"
                     /translation="MQLAEKTLPSLTFDDVNRLAAADMRAVDELIRESLKSDVALVSQ
                     VSEHIVTSGGKRLRPLIVLLAARALGYTGRHHVHCAAIIEFIHTATLLHDDVVDSSAR
                     RRGRDTANTVFGNQASVLVGDFLYSRAFQMMVSVGRMRVMQILADATNTIAAGEVMQL
                     MNVHDPDVSEDSYRQVIYRKTARLFEAGAQIAAVLANRDAADEAAMVRYGQNLGTAFQ
                     LIDDALDYDAMPDELGKNLGDDLAEGKTTLPLIYAMENGSDSEREMIRSAIETDGLPR
                     LRDIQSVIESTGALRYTSARAQEAADAAIDALSGVPESEYKQAMIAIAEFAVKRRT"
     gene            1628..1936
                     /gene="rplU"
                     /locus_tag="VFY27_08390"
                     /pseudo
     CDS             1628..1936
                     /gene="rplU"
                     /locus_tag="VFY27_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            2253..2510
                     /gene="rpmA"
                     /locus_tag="VFY27_08395"
     CDS             2253..2510
                     /gene="rpmA"
                     /locus_tag="VFY27_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5765573.1"
                     /translation="MAHKKAGGSSKNGRDSESKRLGVKVFGGQTVVAGNIIVRQRGTR
                     FHPGVNVGIGRDHTLFAKSNGEVKFEKKGPKNRQFVSVHAG"
     gene            2588..>3483
                     /gene="obgE"
                     /locus_tag="VFY27_08400"
     CDS             2588..>3483
                     /gene="obgE"
                     /locus_tag="VFY27_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5765574.1"
                     /translation="MKFVDEATISVQAGNGGHGALSFRREKYIERGGPDGGDGGRGGS
                     VYLVARAGTNTLADFRVGRKYRAPSGQPGAGNNKSGKSGEDLDVIVPCGTVVHDVDTG
                     ELIGDLTEAGQRLKVAEGGRGGLGNTHFKSSVNRAPRKTTQGTPGESRHLKLELKLLA
                     DVGLVGLPNAGKSTLIRAMSAARPKVAAYPFTTLHPHLGVVAVGPLQSFVMADIPGLI
                     EGAAEGAGLGMQFLRHLERTRLLLHLVDIAPLDASTNPADQIKAIEEELKKFSADLAA
                     KPRWLVLNKVDLLPAEDLPEAR"
BASE COUNT          728 a         1022 c         1103 g          630 t
ORIGIN      
        1 gcgttgcggt gaccgtacca gccggagatc cctaagccgc cgaacaggtc gagtatcggg
       61 tccggattga actcgcggct caggatgatc cacatgtcga tgctctgccg gcgcatggca
      121 tcacgcaggt agaggtccat cttttccttc ttcaccgccc actcatgaag ttcgcgcgcg
      181 agcgcggatc cgctgtgctg gcttggcgaa agctggggca cctcggattg ctccgcggcc
      241 agaacgggtt gcaccaggca ggcaaggaaa agcgtaacgg tcacggtgag cgttcgccgc
      301 acactgcggc aagcagttga caacttgctg ttcaggggag ccctcgcagt atcaagctag
      361 aaaagtgtca cgtgcggcgt ttgacggcaa attcagcaat cgcaatcatc gcctgtttgt
      421 attccgattc cggcaccccc gagagggcat cgatggcggc atcagcggcc tcctgcgccc
      481 gcgccgatgt gtagcgcaac gcgcccgtcg attcgatcac cgattgaatg tccctgagcc
      541 tgggcaagcc gtccgtctcg attgccgagc gaatcatctc gcgttcgctg tcgctgccgt
      601 tttccatcgc ataaataaga ggcagcgtcg tcttgccctc cgcaagatcg tctcccaggt
      661 tcttgcccag ttcgtcgggc attgcgtcgt agtccagtgc gtcatcgatg agctggaacg
      721 ccgtgcccag gttctggcca tagcgcacca ttgcagcttc gtccgccgcg tcccggtttg
      781 cgagcaccgc cgcaatctgt gcgcccgctt cgaacagccg ggcggtcttc cgatatatga
      841 cttgccgata gctgtcttca ctgacgtccg ggtcgtgcac gttcatcaac tgcatcactt
      901 cgccggctgc gatcgtattg gtcgcatcag ccaggatctg cataacccgc atccggccaa
      961 ccgacaccat catctggaag gcccgcgaat acaggaaatc tcccacaagc acgctcgcct
     1021 ggttgccgaa gacggtgttt gcggtgtcgc gtccacggcg tcttgctgaa gaatcgacga
     1081 catcatcatg gagcagggtg gcggtgtgga tgaactcgat aatcgcagcg caatgcacat
     1141 gatgtcggcc ggtgtacccg agcgcacgcg ccgcaagcag gacgatcagc gggcgaagcc
     1201 ttttgccgcc gctcgtgacg atgtgttccg atacctgcga caccaacgcc acatcgctct
     1261 tcaagctttc ccgaatcagc tcgtcgacag cccgcatgtc ggcggcagcc aaccggttga
     1321 cgtcgtcgaa ggtcagcgat ggaagtgtct tttcggcgag ttgcatgtca tcaggataat
     1381 gcctgaatcg cagttccaag gcgacagcgg ccgccggctg cgtgcgcggc ggcaagcctt
     1441 tgaaatgagt taagattcaa acctgccata cgttgacccg cggctgccga atctataaaa
     1501 ttcccgctct tttgtggcgg caggcttcca ggcggtccag gcttcaagcc ctcttcgtcc
     1561 gcagaaatct ctttattctc agcaggttgt gcggcttcgc gcgacctgct caacggagca
     1621 catatcgatg tacgcggttt ttcgagccgg cggtaagcaa taccgcgcga gcaagggcga
     1681 gcgtctgaag ctggaaaagt tcgcagccga cgaaggcgcg aacgtcgagt tcgacgaagt
     1741 attgctggtg ggcgagggca gcgacgtcaa ggtcggcagt cctttggtcg acggaaccat
     1801 cgtcagcgcg aaagttctca agcagggcaa gagcggcaag gtaaccgtca ccaagtttcg
     1861 ccgtcgacag aactacctgc ggcagcacac gcaccgccag tggtttacgg aagtcgagat
     1921 caccgggata acgggcgaga aggaagagcc ggcgaaggcg cagtcggagg acgcttcggc
     1981 agccggcgag ccggccccga agaagacggc caaaaagtcc tcgaccagga aaaaggcgac
     2041 gaaaaaggca gcgggttcgt cttcgtctgc caagaagacg ggtaagacgt cttcaaagaa
     2101 gaagacggcg aagaaaacgt aactgtagga acgtcccgcg cgggctgcga tttgcatcag
     2161 cgaactcgcc cgcacggcgg gctcctacag acggatcaga gaatggtttc gatgcaccag
     2221 gtgtcggacc cgaacattca aggtgaagca aagtggcaca caagaaagca ggcggtagca
     2281 gcaagaacgg tcgcgattcg gagtcgaagc gcctcggcgt gaaggtcttc ggcggccaga
     2341 cggttgttgc cggcaacatc atcgttcgcc agcgcggcac ccggtttcat ccgggagtga
     2401 acgtgggaat cgggcgcgac cacacgctgt ttgcgaagag caacggcgaa gtgaagttcg
     2461 agaaaaaggg cccgaagaac cggcagttcg tgagcgtcca cgccggctga acgggttcac
     2521 taccagaacg cgcgaagaac cccgctgctg cggggttttt tgtatgcatc cactttagaa
     2581 tttgcccatg aaattcgtag acgaggcgac catcagcgtc caggccggca acggcgggca
     2641 cggtgcgctc agcttccggc gggaaaaata catcgaaagg ggtgggccgg acgggggcga
     2701 tggcggccgc ggcgggagcg tctacctggt ggccagggcc ggtaccaata cgctcgccga
     2761 ttttcgcgtc ggccggaagt accgcgctcc gagcggccag cccggcgccg ggaataacaa
     2821 gagcggtaag tctggcgaag acctggatgt gatcgtgcct tgcggcacgg ttgtgcacga
     2881 cgtcgacacc ggcgagctga tcggtgacct gacggaagcc ggacagcggc tcaaggtggc
     2941 ggagggcggc cgcggcggac tcggcaacac tcacttcaag agcagcgtca atcgcgctcc
     3001 acgcaagacg acccagggta cgccgggcga atcgcgacac ctgaagctcg aactgaagct
     3061 gctggcggac gtggggctgg tcggcttgcc gaacgcgggg aagtccaccc ttatccgggc
     3121 gatgtcggcg gcccgcccga aggttgctgc ctatccgttc acaaccctgc atccgcacct
     3181 gggcgtcgtc gccgtgggtc cgttgcagag tttcgttatg gcggatatcc cgggcctgat
     3241 cgagggtgct gccgaaggtg caggcctagg catgcaattc ctgagacatc tagaacgcac
     3301 gcggctcttg ctgcacttgg ttgacattgc gccgctggac gcaagcacca acccggccga
     3361 tcagatcaag gcgatcgagg aggaactgaa gaagttctcc gccgacctgg cggcgaagcc
     3421 aaggtggctg gtgctgaaca aggtggatct tttgccggca gaggacctgc cggaggctcg
     3481 cga
//
LOCUS       DASUOM010000402         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1172672,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000402 DASUOM010000000
VERSION     DASUOM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172672"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFY27_08405"
     CDS             <1..577
                     /locus_tag="VFY27_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5765575.1"
                     /translation="YVALGAGDVTSSAVANAVQRLRKEEPEAPLPAKRQQRRKGSVNE
                     QVEIRGVGDLLCTLARCCRPVPPESIVGYITKGRGVGIHRSNCRNFRSLYSRHPERVI
                     EVDWLDPADENYAVELVLQAYDRQGLLRDVSGVLADQKVSIDSIRTSTDKQRMQASME
                     LKIYVPGLAALSSIIGKLEQLPNVTSVRRKS"
     gene            778..>4433
                     /locus_tag="VFY27_08410"
     CDS             778..>4433
                     /locus_tag="VFY27_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5765576.1"
                     /translation="MTTGHSPEIDTSTPPPDDDAAGASSCPPMDAFPVVGIGASAGGL
                     EAFLALLKHLPADTGMAFVLIQHLDPKHESSLVDLLAKVTEMPVQLATEGLKVAPDNV
                     YVIPPNTNMRLAERCLRLTPRNEARGQHLPIDYFFRSLSNDKQSRSVGVVLSGTGSDG
                     SLGLSEIKAAGGLTFAQDLESAKYDGMPKSAAHTGAVDLVLTPEKIARELARIGRHPY
                     LGPEDTEEPEPSRSDKGEDEDLIRRILHLLRSSCGVDFSLYRKTTIRRRIMRRLVLHP
                     EDTLADYARKLEGDRAELEALFEDLLINVTSFFRDPEMFRELAESVFPAIVRSKSAGQ
                     AIRIWTAGCSTGQEAYSIAMALSEFLDDKTDTPEIQVFATDLSDAVSLAKARAGHYPD
                     SIEGEVSPERLRRFFRKENGGYRVVKAIRDLCVFAKQNIAQDPPFSRMDLISCRNVLI
                     YLSPALQKRIIPTFHYALNPGGFLLLGASETVGGFSELFEAVDKTHKIYARKATTQRV
                     YPHFSAGRQILRAPANDDAVQPPPTLTEWQKEADRLLVSQYAPVGVLVNDRLEVMQFR
                     GRTSPYLEPAPGDASFNLLKMAREGLFVELRSGIAEARDKDAGVERTGVGVRDNGHTR
                     FVDLKIVPLHLPGTTAASFLVLFEEPRPPDAGERREKDRRAGAQASRRATWPRGMAFR
                     RKLGKLLPGAADRSGKSAASAPPGDDAAQPEQKELEQLRRELAATRDHQQSILEQQEA
                     ANEELRSANEEVLSSNEELQSTNEELETAKEELQSLNEELTTVNEQLQSRNLELNRLN
                     NDLSNFFGSTQIPLVMLGTDLKLRRFTPAAEKAFNLHEADVGRPLGDIRLSADVPDLE
                     ALVLETQETMQLREREVQDRAGRWHLLRVRPYRTTENKIEGAVVALIDIDEIKRSQQR
                     LKEAAEYAEAIVATVRVPLLVLNAGLRVQSANKSFYRTYQVKAEETENRHVYELGDGQ
                     WDIPDLRSLLDGILARGKTVEDYEVEHDFPSIGHRTMLLNARALQRKEGGSDLILLAL
                     EDVTERKRLRRDQRALLAREQAANERLRESEGRYHHLVENLPAAVYTCDAEGRLTFYN
                     ETAAALWGREPEIGKEFWCGSWRLYRSDGTPLPHEDCPMATAIREGRAVSSEVVIERP
                     DGTRTDGYAQATAIFDASGAVTGAINLVTDNTERKLSEKAKADLAAIVESSDDAIVSK
                     DLDGVIRSWNRGAER"
BASE COUNT          950 a         1370 c         1375 g          738 t
ORIGIN      
        1 gtatgtcgcg ctgggagccg gcgatgtcac ttcctccgcg gtggcaaatg ccgtccagcg
       61 cctgcgcaag gaagaacctg aagcgcccct gccggcaaag cggcaacagc ggcgcaaggg
      121 gtcggtcaac gagcaggtcg aaatccgcgg cgtcggcgac ctgctgtgca cgctcgcccg
      181 ctgctgccgt ccggtaccac ccgagagcat cgtcggatac attaccaagg gccgcggggt
      241 cggcatccat cgcagcaact gcaggaattt ccgcagtctc tacagccgtc atccggaacg
      301 cgtcatagaa gtcgactggc tcgatcccgc cgacgagaat tacgccgtcg aactcgtttt
      361 gcaggcctac gaccgccagg gcttgctgcg cgatgtaagc ggcgtgctgg ccgaccagaa
      421 agtcagtatc gacagtatcc ggaccagcac cgacaagcag cgcatgcaag cgagcatgga
      481 gctcaaaatc tacgtaccgg gcctggccgc actaagcagc atcatcggca aactcgagca
      541 attgccgaac gtcacctcgg tgcgacgcaa gagctgataa acggggatga ggggaaaaat
      601 ttacttctgg cggggccgat atggcctaga ataagaagac cgaagaaaat aaatctgtct
      661 cccttgtgcc aattggcgca ccaggcacgt tatgccgctt cagggtcaag ccaccttggc
      721 accgacgcgg gggcatcctg atcgtcctca gcccgcactt ggcacgcaga gatcatcatg
      781 accaccggtc acagcccgga aatcgacaca agcaccccgc cgccggatga cgacgccgcg
      841 ggcgccagct cgtgcccacc gatggatgcg ttcccggtcg tcggcatcgg cgcctctgcg
      901 ggaggactgg aagcctttct tgcgcttctg aaacacctgc cggcggacac cggcatggcg
      961 ttcgtgttga ttcagcacct cgacccgaag catgagagtt cgctggtcga cctgctggcg
     1021 aaagtcactg aaatgcccgt tcaacttgca accgagggat tgaaagtcgc gccggacaac
     1081 gtgtacgtca tcccgcccaa cacgaacatg cggctggcag aacgttgctt gcgcctgaca
     1141 ccgcgcaatg aggcgcgcgg acagcatctg ccgatcgatt acttttttcg ctcgttgtcg
     1201 aatgacaaac aatcaagaag cgtcggcgtg gtgctgtccg gaaccggttc cgacggtagc
     1261 cttggacttt cagaaatcaa agccgccggt ggcctcacgt tcgcgcagga cctggagtca
     1321 gccaagtacg acggcatgcc gaagagcgcc gctcacaccg gcgcggtcga tctcgtcctg
     1381 acaccggaga aaattgcccg ggaactcgcg cgcatcggcc gccatcctta tcttggtccc
     1441 gaggataccg aggagccgga accgtcgcgg tccgacaagg gtgaggatga ggacctgatc
     1501 cgaagaattc ttcacctgct tcgttcatcg tgcggcgtcg atttcagcct gtaccggaag
     1561 accaccatca ggcgacgaat catgcggcgg ttggtcctgc accccgagga cacgcttgcc
     1621 gattacgcgc ggaagctcga aggtgaccgc gccgagctgg aagcgctctt cgaggacctc
     1681 ctgatcaacg tcaccagctt tttccgcgac ccggagatgt tccgggaact cgccgagagc
     1741 gtttttcccg cgatcgttcg gagcaagtca gcaggacaag cgatccgcat ctggacagcc
     1801 ggctgttcca cgggccagga agcctattcg atcgcgatgg cgctatcgga gttcctggat
     1861 gacaaaacgg acacgccgga aatccaggta tttgccacag atctcagcga tgccgtctca
     1921 ctggccaagg cccgcgccgg acattatccg gacagcatcg agggggaagt ctcgcccgaa
     1981 cgcctgcggc gattcttccg caaggaaaat ggcggctacc gggtcgtcaa ggctatccgt
     2041 gacctgtgcg tattcgccaa gcagaacata gcccaggatc cgccattttc ccgcatggac
     2101 ctgatcagtt gccgaaacgt cctgatctat ctgtcaccgg cactgcagaa gcgcatcatc
     2161 ccgacctttc attacgccct gaatcccggg ggcttcctgc tccttggggc gtccgaaacc
     2221 gtcggcggct tctcagagct gtttgaagcc gtcgacaaaa cgcacaagat ctatgcccgg
     2281 aaggcgacca cccagcgcgt ctacccgcat ttcagcgctg gacgccagat cttacgggcg
     2341 cccgctaacg acgatgctgt gcagccgccc cccaccctga cggagtggca aaaagaggcc
     2401 gatcggctgc tggtcagcca gtatgctccc gtcggcgtac tggtgaacga ccgtctcgag
     2461 gtcatgcaat tccgggggcg gaccagtcct tacctcgagc cggcgccggg agacgcgagc
     2521 ttcaatctcc tgaagatggc gcgcgagggc ttgttcgtgg aactcagaag cggcatcgcg
     2581 gaagcgcggg acaaggatgc gggggtcgag aggacgggcg tgggggtgcg cgacaacggt
     2641 cacacccggt tcgtcgacct gaagatcgtt cccctccatc ttccgggcac gacggctgcc
     2701 tccttcctgg tgctattcga ggaaccccgc ccgccggacg ccggcgagcg gcgcgagaaa
     2761 gatcgacgcg cgggcgcgca ggcttcccgc cgcgccacct ggccgagagg aatggctttt
     2821 cgccggaagc tgggcaaact gctgccggga gcggccgacc ggtcaggcaa gtctgctgcg
     2881 agcgcgccgc cgggcgatga cgcggcacag ccggagcaga aggaactcga gcaactgcgg
     2941 cgtgaactgg cggcaacgcg cgaccatcag caatccatcc tggaacagca ggaggccgcc
     3001 aacgaagagt tgcgctcggc caacgaggag gttctgtcct ccaatgagga actccaaagt
     3061 accaacgaag aactggaaac tgcgaaggaa gaactgcagt cgctgaacga agaactgacg
     3121 acggtcaacg aacaactgca gtcccgcaac cttgagttga accggttgaa caatgacctg
     3181 agcaacttct tcggcagcac ccagatcccc ctggtgatgt tgggtaccga cctcaagctg
     3241 cggcggttta cccctgcggc cgagaaagcc ttcaatctgc acgaagcgga cgtcggccgg
     3301 ccgctcggcg atatccggtt gtccgccgac gttcccgacc tggaagcgct cgtgctcgag
     3361 acgcaggaga cgatgcagct tcgcgaacgg gaggtgcagg accgcgcggg acgatggcat
     3421 ttgctgcgcg tccgacctta ccgcaccacc gagaacaaaa tcgagggagc agtcgtcgcg
     3481 ctcatcgata tcgacgaaat caagcgaagc cagcaacgac tgaaggaggc cgccgagtac
     3541 gcggaggcca tcgtcgccac ggtgcgggtt ccgctgctcg tgctgaacgc cggattgcga
     3601 gtccagtcgg caaacaagag cttttaccgg acgtatcagg tcaaggcgga ggagaccgag
     3661 aaccggcatg tgtatgagct gggagacggt cagtgggaca tccccgactt gcgctcgctg
     3721 ctggatggga tccttgcgcg cggcaagacc gtggaggatt acgaggtaga gcacgatttc
     3781 ccgagcatcg ggcataggac catgctgctg aatgcgcgtg cgctgcaacg gaaagaaggc
     3841 ggatcggact tgattctgct cgcattggaa gatgtgaccg aacgcaagcg gctgcgtcgg
     3901 gatcagcgtg cattgctcgc gcgcgaacag gcggccaacg aaagattgcg cgaaagcgag
     3961 ggtcgctacc accacctggt ggagaatctt cccgcagcgg tctatacctg cgatgccgaa
     4021 ggccgcctca ccttctacaa tgagacggcc gccgccttgt ggggccgaga accggaaatc
     4081 ggcaaggaat tctggtgtgg gtcctggcgc ctttaccgct cagacgggac tcccctgccc
     4141 catgaggact gcccgatggc caccgctatc cgggagggac gggccgtcag tagcgaagtc
     4201 gtgatcgaac gccccgatgg cacccgaacc gacggctatg cccaggctac tgccatcttc
     4261 gatgcttcgg gcgcggtgac aggggcgatc aacctggtga cggacaacac cgagcgcaaa
     4321 ttgtccgaga aggcaaaggc ggatctcgcc gccattgtcg agtcctccga cgacgcaata
     4381 gtgagcaaag acctcgacgg cgtcatccgg agctggaacc gcggcgcgga acg
//
LOCUS       DASUOM010000403         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1106515,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000403 DASUOM010000000
VERSION     DASUOM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106515"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="alr"
                     /locus_tag="VFY27_08415"
     CDS             complement(<1..905)
                     /gene="alr"
                     /locus_tag="VFY27_08415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5765577.1"
                     /translation="MTCGARAVIHLDALRFNFKRIREAANGARILAVVKADAYGHGLA
                     TVATNLPDADGFAVARIDEALQLHATGVRKPIVLLEGVTDAEEMQAALQRKFQPVVHC
                     EQQIQLLEEAAAGRTGVWLKIDTGMHRLGVEPARVAELIERLRACRSVLELGMMTHLA
                     TADDPSDGTTLRQLATFRDLVGRFAGDVSIANSAAIFGWSDAITTDLPPGRTWVRAGV
                     CLYGISPFEGSCGRDLGLRPVMEFATSLIAVKAIRAGDRVGYGATWEAAEDTILGIFA
                     AGYGDGYSRFIPSGTPVLVNGRRAKL"
     gene            complement(902..2296)
                     /gene="dnaB"
                     /locus_tag="VFY27_08420"
     CDS             complement(902..2296)
                     /gene="dnaB"
                     /locus_tag="VFY27_08420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5765578.1"
                     /translation="MARAFDDRPPDAAEQRLKVPPNSLEAEQSLLGGLMLDNDAWDKI
                     ADVVIAEDFYRKDHRLIFAAIAELAEDSNPCDVITVSEHLDQRNQLEKAGGLQYLATL
                     ANEAPGASNVRAYANILRERSMLRSLINAGNEISGSAFSTDGRTAAELVDEAERRVFE
                     IADKGSRGRVGFKSLKQILPETVDRIDLLHQSGGDITGLPTGYHEFDKMTAGLQPGEL
                     IIIAGRPSMGKTTLAINIAENVSIGHKVPTAIFSMEMPSQQLAFRMISSLGRVDQTHL
                     RTGRFPDEDWSRINTAVQLMSDAPIFIDDSPALSPTEIRARARRLKREHGLGLIVLDY
                     LQLMQVHGNTENRATEISEISRSLKALAKELALPIIALSQLNRSVEQRTDKRPVMSDL
                     RESGAIEQDADLIVFIFREEVYNSDTPRKGIADINIAKQRNGPIGDFPLTFVGRFTKF
                     ENWVPESYAEEAYS"
     gene            complement(2410..2859)
                     /gene="rplI"
                     /locus_tag="VFY27_08425"
     CDS             complement(2410..2859)
                     /gene="rplI"
                     /locus_tag="VFY27_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5765579.1"
                     /translation="MNVILLDSVENLGRIGDLVTVKAGFGRNYLLPSGKAALATKENI
                     AEFEAKRAELEKAAAEELKAAKARAELIRGMELVISANAGTEGKLFGSIGTHDISEAF
                     EKVGVEVARSEVRLPDGPIHETGEYNIGVHLHSELDVEVTLRVVPEE"
     gene            complement(2925..3842)
                     /locus_tag="VFY27_08430"
     CDS             complement(2925..3842)
                     /locus_tag="VFY27_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765580.1"
                     /translation="MALRTRLADMTLRPIASWLVARPQNAVIGLAGTLLLPFAQVISG
                     TLMALLVFKHGAALAAMQGAVATAILAIISLLVSASASHVLANALVIWAPVILLVVMM
                     RGWRSLTLTLQVTVIAAMVVTLAFYAILGDPNVFWGEILVRLSDSFREMGLTQHADVL
                     IDQRDVIAPQMTILVVLTTWSLYAMVLLLGYAIYRTLPGVTSNFGRFRDLNFGRVLAL
                     TMAVASVIAMVTGAAWLQNFAFVIFAMFWLQGLAIVHWLHAEGRLPLAVVILVYALIP
                     LLNALLVMGLAVVGYIDAWFGFRSRKPAP"
     gene            complement(3882..4106)
                     /gene="rpsR"
                     /locus_tag="VFY27_08435"
     CDS             complement(3882..4106)
                     /gene="rpsR"
                     /locus_tag="VFY27_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5765581.1"
                     /translation="MSRYSRRRKYCRFTAEGIEEIDYKDLSLLKAYVTETGKIVPSRI
                     TGTKAHYQRQLSTAVKRARYLALLPYTDRH"
     gene            complement(4334..4660)
                     /gene="rpsF"
                     /locus_tag="VFY27_08440"
                     /pseudo
     CDS             complement(4334..4660)
                     /gene="rpsF"
                     /locus_tag="VFY27_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
BASE COUNT          915 a         1551 c         1420 g          996 t
ORIGIN      
        1 agtttcgcgc gccgcccgtt aaccagcacc ggcgttcccg acggaatgaa gcgcgagtag
       61 ccgtctccgt aacctgccgc gaagatcccg agtattgtgt cttccgccgc ttcccacgtc
      121 gcgccgtagc cgacccggtc cccggcgcgg atcgccttta ccgcgatcaa gctggtggca
      181 aactccatca ccggtcgaag gcccagatcc ctgccacaac taccctcgaa cggtgagatg
      241 ccgtacaggc agacgccggc gcggacccag gtccgtcccg gcgggaggtc cgtcgtaatg
      301 gcatcggacc agccgaaaat cgcggcagaa ttcgcgatgc tgacgtcgcc tgcgaatcgg
      361 ccgacaaggt cacggaaagt ggccaactgc cgcaaagtcg ttccatcgga cgggtcatcg
      421 gcggtggcca ggtgagtcat cattccaagc tccagcactg agcggcaggc gcgtaacctt
      481 tcgatgagct ccgccacgcg cgccggctcg acccccagcc ggtgcatacc ggtgtcgatc
      541 ttcagccata ctcccgtcct gccggccgca gcctcttcga gcagctggat ctgttgctcg
      601 caatgcacaa ccggctggaa tttacgctgc agcgcagcct gcatctcttc ggcgtcggtt
      661 acgccttcga gcagcacaat cggcttgcgc acgccggtgg catgaagctg aagggcctcg
      721 tcgatgcgag cgaccgcgaa accgtcggcg tcgggcaagt tggttgccac cgtcgcaagc
      781 ccatggccgt atgcatcggc cttcaccacg gcgagaatac gcgcgccgtt cgctgcctcc
      841 cgaattcgct tgaagttaaa tctgagcgcg tcgagatgta tgaccgcccg ggccccgcat
      901 gtcacgaata ggcctcttcc gcgtaacttt ccggcaccca gttttcgaac ttggtgaaac
      961 ggcccacgaa cgtcaaaggg aaatcgccga tcggcccgtt tcgttgcttg gctatgttga
     1021 tgtctgcgat gcctttgcgc ggcgtatcgg agttgtacac ttcctcacga aagatgaaca
     1081 cgatcaggtc cgcgtcctgc tcgatagcgc ctgactcacg caggtccgac atgaccgggc
     1141 gcttgtcggt gcgctgctcg acgctgcgat tgagctgcga cagcgcgatg atgggaagcg
     1201 cgagctcttt cgcaagcgcc ttcagcgaac gcgaaatttc agagatctcc gttgcacggt
     1261 tttcggtatt gccgtgcacc tgcatcaatt gcaggtagtc gagcacgatc aggcccaaac
     1321 cgtgttcccg cttcaggcgc cgtgctcgcg cgcgtatctc ggtcggcgac agggcggggg
     1381 aatcatcgat gaagatcggg gcgtccgaca tcaactggac agcggtattg attcgcgacc
     1441 agtcttcgtc cgggaaccgg cccgtgcgga gatgtgtctg gtcgacgcgc ccgagcgagg
     1501 aaatcatgcg gaacgcgagc tgctgcgacg gcatttccat ggagaaaatc gcggtaggca
     1561 ctttatggcc gatcgacacg ttctcggcaa tgttgatcgc cagggtggtt ttacccatgg
     1621 aaggccggcc ggctatgatg atcagctcgc cgggctgaag ccccgcagtc atcttgtcga
     1681 actcgtgata gccggtgggc aggccggtta tgtctccgcc cgactggtgc aacaggtcga
     1741 tgcgatcgac cgtttccggc aggatctgct tcagcgactt gaaccccact cgcccgcgcg
     1801 agcccttgtc cgcgatctcg aacacccggc gctcggcttc atccacgagc tcggcggccg
     1861 tcctgccgtc cgtggaaaag gcgctgccac tgatttcgtt gccggcgttg atgagcgacc
     1921 gcaacatcga acgttcgcgc aggatgtttg cgtaagcgcg tacgttggac gcgcccgggg
     1981 cttcattcgc gagagtggcg agatactgga ggccgcctgc cttctccagc tggttccgct
     2041 ggtcgagatg ttcggaaacg gtgatgacgt cgcatgggtt gctgtcttcc gccagttccg
     2101 cgatcgctgc gaatatcagg cggtgatcct tgcgataaaa atcttccgcg ataacgacgt
     2161 cggcgatctt gtcccaggcg tcgttatcca gcatcaggcc gccgagcaac gactgctcgg
     2221 cctccaggga gttcggcggg accttcaggc gctgctcggc tgcgtccggt ggtctgtcat
     2281 caaacgctcg agccattaat tgttatccat ccgtatccac ggctgaactg cgtccccggc
     2341 ggcggcgcgt gcctttgttg tgtgtgacgt taaggtacca ctcccgtcac gcgcgaagaa
     2401 agaatatctc tattcttcgg gcacgactct caacgttacc tccacatcca gctccgaatg
     2461 caggtggacg ccgatgttgt actctccggt ctcgtgaatc gggccgtccg gcaggcggac
     2521 ttcgctgcgc gccacttcca cacccacctt ctcaaaggct tcggaaatgt catgcgtacc
     2581 aatcgagccg aacagcttac cttcggtacc cgcgttggcg gatatgacca gttccatgcc
     2641 gcgaatgagt tctgccctcg cctttgccgc cttcaactct tccgcggctg ccttctcgag
     2701 ttccgcacgc ttcgcctcga attcggcgat gttttccttt gtcgccagcg cggctttgcc
     2761 ggagggcagg aggtagttgc ggccgaagcc ggccttcacc gttaccaggt cgccgatcct
     2821 gccgagattt tccacactgt ccaataagat aacgttcatt tttgaaaccc tgtttcgtat
     2881 ttcatcgttt tgacatttat taagaactct gccgatgcgg ctctttaagg tgccggcttc
     2941 cgcgaacgaa aaccgaacca cgcgtcgatg taacccacaa ccgccagtcc cattaccagt
     3001 aatgcattga gcaacggtat cagggcgtac acgagtatta ccaccgccag cggcaatcgt
     3061 ccctccgcgt gcagccaatg gactattgca agcccttgca gccagaacat cgcgaagatg
     3121 acgaacgcga aattctgcag ccatgcggct ccggtgacca tggcgatcac cgacgcgacc
     3181 gccatagtca gcgcgagcac gcggccgaaa ttcaagtcgc gaaaccggcc gaaattgctg
     3241 gtaactccgg gcaacgtacg gtaaatggcg tagcccaaaa gcagcaccat cgcatagagc
     3301 gaccaggtcg tcagcacgac caggatagtc atctgcggcg cgatcacgtc acgctgatcg
     3361 atcaaaacgt ccgcgtgctg ggtaagtccc atctcgcgga acgagtccga cagccggacc
     3421 agtatctcgc cccagaacac gttcgggtcg ccgagtattg cgtaaaacgc cagcgttact
     3481 accatcgcgg caatgaccgt cacctgcaag gtgagcgtca gcgatctcca gccgcgcatc
     3541 atcacgacca gcaggatgac cggcgcccag atgaccagcg catttgccag cacgtgcgac
     3601 gccgaagcgc ttaccaacag cgaaataata gccagtatcg cagtcgcgac agcgccctgc
     3661 attgctgcca gcgccgcacc atgcttgaag accaggagcg ccatcaacgt gccgctgata
     3721 acctgcgcga acggcagaag caacgtgccc gcaaggccga tgactgcgtt ctgcggccgg
     3781 gctaccagcc acgatgctat gggacgcaag gtcatgtcag caagccgcgt acgcagggcc
     3841 actgccccgg ctcgaatgcc ccgggcacct gcacgcggcg ctcagtgccg gtcggtgtac
     3901 ggcagcagtg cgagataacg cgcgcgtttt accgcggtcg acagctggcg ctggtaatgc
     3961 gccttcgtgc ccgtgatccg gctcggcacg atttttccag tctcggtaac gtacgctttg
     4021 agcagtgaga gatccttgta atctatctct tcaatgccct ccgccgtgaa gcggcaatac
     4081 ttccgcctgc gtgaatatcg gctcatcttt gcaatctccc tcaggcgcta cgggcggcag
     4141 cgcgtgaatc ctcgtcatcg gcctgttcac cgtcacgatc ttcggcattt tcatccgaat
     4201 ccgtatcggc ttcgacgtct gcccgctcgc tgcgaccggt gtcgtcgccg tcgtcatcgc
     4261 tgccgtcggt tgccgccgca tccgtggcgg cccttgcgtc acgtcgccgc tgcgattcac
     4321 gttcccgggt cttctcttcc tcgacctgga cggccatcgg agacgcttcg atgattgcct
     4381 tgtcgcgtgc aatgataagg tggcgcagca ccgcgtcatt gaagcggaat gcactgcgga
     4441 tttcctcgat cgcttcgttg ccgcattcca cgttcatcag gacgtaatgc gctttatggg
     4501 ctttgtggat cgggtgtgcc agctggcgcc ggccccagtc ttcgttgcgg tgcactgcac
     4561 cgccggagcc ggtcacgatg ccctggtact tttccaccat ggcaggaacc tgctcgctct
     4621 ggtccgggtg aaccaggaac acgatttcgt aatgtctcat tttatcccct tacggattag
     4681 agcctcccgg ccgtcccggt gaggcaagag ggtcaggcgc ctgattttga gtcgccggtg
     4741 ccggcgagat ggatcgggcc cgtccgccgc gggcggaggg acgcgcatcc tagcgaagca
     4801 cgtggggttc tgcaagccgg ctaagcctcg cgcgacgatc agcagcgtcc atgaagccac
     4861 gacgaacggg cggagtgttg at
//
LOCUS       DASUOM010000404         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_15182,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000404 DASUOM010000000
VERSION     DASUOM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15182"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VFY27_08445"
     CDS             <1..1584
                     /locus_tag="VFY27_08445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5765582.1"
                     /translation="MPWVALKFARAAAGNHAATATYLRQEFLNLSQLNHPNIVSVFDF
                     DSDGGLEFIVMEWLEGQTLADMLSQITSKRIALHKADEIVHAVAGALAHAHERGVVHG
                     DVKPSNIFLTGNRAVKLLDFGSSMQASADADTEGNWATRAYASCEVLRGALPQPHDDV
                     YALGVTAYRLMSGEWPFGDLDASEAQTTDSVVSPLPPDAERSWHAIEHALRFDARDRP
                     AHAGEFLMELRDPPLETAEPRERSQLEHIAYGAVAAALLIALVVLTVGSIGGLPAQQQ
                     DVLERAEAAFADGRLVGDDSALEQYSSVLQLSPDHPVAVAGLQSIAERYLANARQALA
                     EDDPGTARGLLSTARQVMPGHYGIPITEDLIARFGKDLLFRARGAAATDLEGAERLLA
                     DASAFLPADDAGVVAVAAQLEQMRKEATLQELLQGIDQRILAERLSLPSGDSAMDLLR
                     RARELAPGDRQVMLAADRIATALLFQSMFAISSGKLDDAERFIDEAKSLNVEHLALSR
                     AEYELAKARHDDVRTRRSR"
     gene            complement(1607..2098)
                     /locus_tag="VFY27_08450"
     CDS             complement(1607..2098)
                     /locus_tag="VFY27_08450"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX5765583.1"
                     /translation="MFRDNSLKRAGFPLALLASLALGAGCAEQRERGIDESDAGQIIG
                     GVAGAAAGSQVGGGSGRTVAMIAGALIGSMAGERIGARMEEDDMQEAGFALEENRTGE
                     THAWENPDTGNEFRITPTDTYERDGRPCREFDFRVETEHGSDVQERTACRDSDGTWEV
                     IDS"
     gene            complement(2259..2504)
                     /locus_tag="VFY27_08455"
     CDS             complement(2259..2504)
                     /locus_tag="VFY27_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765584.1"
                     /translation="MKKVVAIVGGLALASGIAFAGDGEKKTFNDLDQNQDGVITQSEA
                     ASDEKLVSEFTTADTNQDGYLTPSEFEALEGEMEEAE"
BASE COUNT          522 a          908 c          919 g          587 t
ORIGIN      
        1 atgccttggg tcgcattgaa gtttgcgcgg gcggcggcgg ggaatcatgc ggcgactgcg
       61 acttaccttc gacaggaatt cctgaacctc tcgcagctca accacccgaa tatcgtcagt
      121 gtttttgatt ttgacagcga cggtgggctc gaatttatcg tgatggaatg gctagagggc
      181 cagacgctcg cggacatgct gtcgcagatc accagcaagc gaatcgcgct tcacaaggcc
      241 gatgagatag tgcacgctgt cgccggcgcc ctcgcccatg cgcatgagcg cggtgtcgtg
      301 catggcgacg taaagccttc gaatatcttc ctgaccggca accgcgcagt aaaactgctc
      361 gacttcggct cgtcgatgca ggcttccgcc gatgcagaca ctgaagggaa ctgggcgacg
      421 cgggcgtacg cgagctgcga agtgttgcgg ggagcattgc cgcagccaca cgacgacgtg
      481 tacgctttgg gtgtcacggc atatcggctg atgagcggcg agtggccgtt cggtgaccta
      541 gatgcgtcag aggcgcagac cacggattcc gtcgtaagcc cactgccgcc cgatgcggag
      601 cgcagctggc atgcaataga gcatgccttg cgtttcgatg ctcgcgatcg ccctgcgcac
      661 gcaggagagt tcctgatgga gcttcgcgat cctccactcg aaaccgcgga accgagagag
      721 cgatcgcagc tcgagcacat cgcctacggg gcggtcgccg ccgcgttact gatagcgctg
      781 gtagtcctga cggttggatc gataggcgga ttaccggcgc aacagcagga tgtgctggaa
      841 agggcggagg ccgcgtttgc cgacggccgc ctcgtcggtg acgacagtgc gctcgaacag
      901 tattcgtcgg tgctgcagct gtcgcccgat catcctgtcg cggtcgccgg actgcagagt
      961 attgccgagc gctaccttgc caacgcccgg caggcgctcg ctgaggatga cccgggaacc
     1021 gcccggggac tgctctcaac agcccggcag gtgatgcccg gtcactatgg cattcccatt
     1081 accgaagatc tcatcgcgcg ctttggcaag gacttgttgt tcagggcgag aggagccgct
     1141 gccaccgacc tcgaaggggc cgaacggctg ctggcggatg cctccgcttt tcttcctgcg
     1201 gatgacgccg gcgtggttgc cgtcgccgcc caactggagc aaatgcgcaa ggaggcgacc
     1261 ttgcaggaac ttttgcaggg gatcgaccag cgcatcctgg cggagcggct gtcccttccg
     1321 agcggtgaca gcgccatgga cctgctgcgc cgggcgaggg aactcgcgcc gggcgaccgc
     1381 caggtgatgc ttgcagctga tcgcatcgca acggcgttgc tcttccaatc catgttcgcc
     1441 atctccagcg gtaagctcga cgatgcagag cgcttcatcg acgaagcaaa atcgctcaac
     1501 gtcgaacacc tggcgctttc gcgcgccgag tatgaactgg cgaaagcgcg ccacgacgac
     1561 gtgagaaccc gccgctctcg ttgagaactg tgccagccgg cggtcgctag ctgtcgatga
     1621 cctcccatgt gccatcggag tcgcggcaag ccgtccgctc ctggacgtcc gagccgtgct
     1681 ccgtttccac gcggaaatcg aactcgcgac agggccgccc gtctcgttcg taggtgtcgg
     1741 tcggcgtaat gcggaattca ttgccggtat ccgggttctc ccatgcgtga gtttcgccgg
     1801 tgcgattctc ctccagggcg aagcccgcct cctgcatgtc gtcttcttcc atgcgcgcgc
     1861 cgatgcgctc gccggccatg ctgccgatga gggcgccggc aatcatcgcc acggtgcggc
     1921 cgctgccgcc gccgacctgg ctgccggctg ccgcacccgc gacaccacct atgatctggc
     1981 cggcatcaga ctcgtctatg ccgcgctcgc gctgctctgc gcaaccggcg cccagcgcca
     2041 gggatgccaa caaagccagg ggaaagcctg ctcttttcag ggaattgtct ctgaacatga
     2101 tgtttttact ccgggttaga cacacgaaca aaggctgcaa gagacatgcc ataggtaagg
     2161 tcaagttatt gattgtaaaa gcttctacaa cagaaaaggg ccccgtccct ggggcccttt
     2221 tcctgtcgct actggtaggg tgaggcgggt attgaagctt actccgcttc ttccatctcg
     2281 ccctcgagag cctcgaactc ggacggtgtc aggtagccgt cctggttcgt gtcagccgtg
     2341 gtgaactccg ataccagctt ctcgtcgctg gctgcctcgg actgggtaat gacgccgtcc
     2401 tggttctggt cgaggtcgtt gaaggtcttc ttctcgccgt cgccggcaaa tgcaatgccg
     2461 ctcgccaggg ccaggccgcc tacgattgct acaactttct tcatgccgtc tactccttac
     2521 gttaagaaac ttatcgcgac tgtcggccgc ataggccgct cgggatactc attgcaaggc
     2581 gcatgccatc ggcggaaaaa gggccggcgg catgaagtca gcgggttgcc gggagggctg
     2641 acggctgttg ttttccttcc cgtacagcgc tttacgtcgg caaaacatgc cgcgccatgc
     2701 accggcttgc tggcgtcgtc attaagagac agtgttgcag aagcgaaatg tagattctgc
     2761 aaaagcaggc aatttgtgcc gcttggccgt cgtcgggcgc ccgctagtgc cgggaagcgc
     2821 tgtctggttc ccccggcagg accacccgct gcggcgagtc ctcgccggtg cgtccctcgc
     2881 cggtgcgtcc ctcgccgaag atgttgtcgc gggcgcggcg attgctcgat ttcctg
//
LOCUS       DASUOM010000405         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_527053,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000405 DASUOM010000000
VERSION     DASUOM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527053"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFY27_08460"
     CDS             complement(<1..273)
                     /locus_tag="VFY27_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEX5765585.1"
                     /translation="MKVDIAASIAEIGATDWNQLAGAEFPFLRHEFLHAAESTGCVSQ
                     ASGWSPRHLALRDRNGRLVAAMPLYEKTHSWGEFVFDWSWAQAYHRA"
     gene            complement(305..1327)
                     /gene="trxB"
                     /locus_tag="VFY27_08465"
     CDS             complement(305..1327)
                     /gene="trxB"
                     /locus_tag="VFY27_08465"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5765586.1"
                     /translation="MSKSRHCRVLILGSGPAGYTAAVYAARANLDPVMVTGIEQGGQL
                     MTTTDVDNWPGDVEGLQGPALMERMLRHAERFGTEVVNDHVKRVDLSKRPFLLEGDYG
                     NYTCDALIIATGATAMYLGLPSEEAFKGRGVSACATCDGFFYRGQQVAVVGGGNTAVE
                     EALYLSNIAAKVTLVHRRDALRAEKILQDHLFQKADQGKVEILWDHVVDEVTGDDAGV
                     TGLRIRRTKSPHSTSDLPVTGVFIAIGHSPNTGLFKDQLEMRNGYIVVRSGLAGDATA
                     TSVPGVFAAGDVADQVYRQAVTSAGAGCMAALDAEKYLEAVDARDSAEPSSISLKKRS
                     ANDAVA"
     gene            1629..>2701
                     /locus_tag="VFY27_08470"
     CDS             1629..>2701
                     /locus_tag="VFY27_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5765587.1"
                     /translation="MTPATRLSDQVVRTLREGALWVFGALALILWVALFTYDPADPGF
                     SQASGSGEINNSVGRVGALVADLLFNFFGRPAYLFTVMVFYFGWMIFREQKTSEHLTR
                     ADFALRFAGFLATLVTSCALATLHFSPLGFRETAGGIIGQVVGGGMASLMKLLGASVL
                     LFFLWLASLSLFLGISWLSIMDRVGRWCLTSWEALQVKLGEFRDRAEGRRKLAARAET
                     VEVEKKRTANRVPPRIEPVIAPLEPSERAQKERQVPLFDPPAAGELPPLSLLDDPPPQ
                     KKGYSEDSLEAMSRLVELKLKDFNIDVEVVSVSPGPIITRFELDPAPGVKVSQISNLA
                     KDLARSLSVVSVRVVEVIPGKSFV"
BASE COUNT          473 a          867 c          836 g          525 t
ORIGIN      
        1 ggcccggtga taggcttgcg cccacgacca gtcgaacacg aattcgcccc aggaatgcgt
       61 cttctcgtac agaggcatcg ccgcaaccag cctgccgttg cgatcacgca aggcgaggtg
      121 ccgcggcgac caccccgagg cttgcgaaac gcagccggtg ctctcggccg cgtgcaggaa
      181 ctcgtgcctc aggaagggaa actcggcgcc cgcgagttga ttccagtcag tggcgccgat
      241 ttccgcgata cttgcagcga tatcgacttt catcctcggg ccagggccac ggccggtggc
      301 cgcgtcaggc gacggcgtcg ttggccgaac gcttcttcaa actgatagac gagggctcgg
      361 cagagtcacg ggcatcgacg gcctcgaggt atttttccgc gtccagcgct gccatgcacc
      421 cggcgcctgc cgatgtcact gcctggcggt aaacctggtc ggcaacgtca cccgcggcaa
      481 acacccccgg tacgctcgtc gcggtcgcat cgccggccag cccggagcgc acgacgatgt
      541 aaccgttccg catctcgagc tggtccttga aaagaccggt gttcgggctg tggccgatgg
      601 cgatgaagac gccggtcacc ggcaggtcgc tcgtggagtg cggggacttc gtcctgcgta
      661 tgcggagtcc ggtcacgccc gcatcgtccc ccgtcacttc atcgacgaca tgatcccaca
      721 ggatctcgac ctttccctgg tccgctttct ggaagaggtg gtcctgcagt atcttttctg
      781 cgcggagcgc gtcccgccgg tgcaccagcg ttaccttcgc ggcaatgtta ctgaggtaaa
      841 gtgcttcctc caccgcggta ttgccgccgc ccaccaccgc cacctgttgc ccgcggtaga
      901 aaaacccgtc gcaggtggcg caggccgaca cgccccgtcc cttgaacgct tcttccgaag
      961 gaaggccaag gtacatcgcg gtggcgccgg tcgcgatgat cagggcatcg caggtatagt
     1021 tgccgtagtc gccctcgagc aggaaagggc gtttggagag gtcgacccgc ttgacgtgat
     1081 cgttcacgac ctcggtacca aaacgctctg cgtgccgcag catgcgctcc atgagagcgg
     1141 gaccctgcag tccctcgacg tctcccggcc agttgtcgac gtctgtcgtc gtcatcaact
     1201 gcccgccctg ttcgatgccc gtgaccatca cagggtccag gttagcccgc gccgcgtaca
     1261 ccgctgcggt atagcctgcc ggtccggaac cgaggatcag aacccggcaa tgccttgatt
     1321 tgctcatgta tacttcgcct tcattttgtg cggcctcgct tcgcctcaag gaaccgactt
     1381 gcgctgcaac ttgcgatatg cgccccggcc ctgcgcgtat caagggccaa ctctgccgga
     1441 tcgtgcgggg tacggggcca gtagtgtacc tcgtcgtaaa aaatgaacca tttgcgcggc
     1501 gccctgcctc gcacgacgct cacctggagc gtgttagtcg agcctctgtg ctccaatatg
     1561 tactaaaatc cgcgcattag agataagagc ttccaacgca cattaaaatg gcaagagccc
     1621 agaagaccat gaccccggcc acacggctgt ccgaccaggt cgtgcgcacc ctgcgtgagg
     1681 gcgccctgtg ggtgtttggg gcgctcgcgc tgattctctg ggtggcactg ttcacctacg
     1741 atcccgccga ccccggcttc agccaggcga gcggctccgg cgaaatcaac aacagcgtag
     1801 gccgcgtcgg ggcgctggtc gcagacctgc tgttcaactt tttcggccgt cccgcctacc
     1861 tgttcaccgt gatggtgttc tatttcggct ggatgatatt tcgcgagcag aaaacgagcg
     1921 agcacctgac gcgggccgat ttcgcgctgc gcttcgccgg tttcctggcg accctcgtga
     1981 ccagctgcgc ccttgccaca ttgcatttct cgccgctcgg ctttcgcgaa accgcgggcg
     2041 ggatcatagg acaggtggtc ggcgggggca tggcgtcgct gatgaagttg ctgggggcga
     2101 gcgtgctcct gtttttcctg tggctggcgt ccctgtcgct gttcctcggc atctcctggc
     2161 tcagcatcat ggaccgcgtt ggccgctggt gcctgacctc atgggaggcc ctacaggtaa
     2221 agctcggcga gttcagggat cgagcagagg gcaggcgcaa gctcgcggca cgggccgaga
     2281 cggtcgaggt cgagaagaag cggactgcaa acagggttcc accacgtata gaaccggtga
     2341 tcgcgccgct cgaacccagc gaacgcgcgc agaaagaacg gcaggtaccg ctgttcgacc
     2401 cgcccgcggc aggggaactg ccgccccttt ctctgctcga cgaccctccg ccgcagaaga
     2461 agggctattc cgaagattcg ctggaggcga tgtcgcgcct ggtcgagctg aagctcaagg
     2521 acttcaacat cgacgtcgag gtggtatcgg tgtcgccggg accgattatt acccgcttcg
     2581 aactggatcc ggccccgggt gtgaaggtaa gccagatatc gaaccttgcg aaggatctgg
     2641 cgcgctcgtt gtccgtcgtc agcgtacgcg tggtggaggt cattccggga aagtccttcg
     2701 t
//
LOCUS       DASUOM010000406         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_491799,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000406 DASUOM010000000
VERSION     DASUOM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491799"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /gene="lon"
                     /locus_tag="VFY27_08475"
     CDS             complement(<1..2354)
                     /gene="lon"
                     /locus_tag="VFY27_08475"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5765588.1"
                     /translation="MSENKPEPAPGSGSPDPTGATSDRLHIVPVRDMVLFPGVIHPVT
                     IRRPASIAAAQAAIRENGKLGLLLQRNAETDAPAPSDLYWVGTVVNILRYVTAPDNAH
                     HLVCQGDTRFRVTNFVQTSPHLIGNFAVPEEATGKEDEVEARVRVLKQQAARALELLP
                     QVPGELGASLRAIDSPGMLCDIIAGFLDIPAAEKQRLLETFDIKQRLDAVLELMAKRF
                     EVLELSHEIEQQTRASIDERQREAVLREQMRTIQQQLGEDEGNAAEFEAIEKAIVDAG
                     MPEEIEEHTRKEFGRLKRMPSAAGEYSMLRNYIDSLTELPWSKLDSESIDIAKARQVL
                     DEDHYGLVDIKKRIIEYLAVRKLNPSGRSPILCFAGPPGVGKTSLGQSIARAVGLKFV
                     RASLGGTHDEAEIRGHRRTYIGALPGKIIQGMRKAGTRNPVFMLDEIDKLGASLHGDP
                     ASALLEVLDPEQNTTFQDNYLGVPFDLSKVMFIATANRLDTIAAPLLDRMEVIELRGY
                     TTEEKLQIAKRYLVARQLENNGLSAEQVEIPDDSLLSIITGYTREAGVRNLEKQIGAV
                     LRHVAVTFAEGAAEARQIGTEDVEKILGSPKFENEVALRTGLPGVATGLAWTPVGGDI
                     LFIECTRVPGSGKLILTGQLGDVMRESAQAAMSLVKSRFADAPEESKLLSGHDMHIHV
                     PAGAIPKDGPSAGVAIFVSLASLVTGQTVRHDVAMTGEISLRGLVLPVGGIKEKVIAA
                     HRAGITRVLLPDRNRKDYEDIPESVRSSLEFIWLKTVEDALDAAL"
BASE COUNT          411 a          791 c          781 g          529 t
ORIGIN      
        1 agggccgcgt ccagtgcgtc ttcgaccgtc ttcagccaga tgaactcgag tgatgagcgt
       61 accgactcgg ggatatcctc gtagtccttg cggttcctgt ccggcaacag cactctcgtg
      121 atgcccgctc ggtgtgccgc tatcactttt tccttgatgc cgcccaccgg cagcaccagg
      181 ccgcgcaggc tgatctctcc cgtcatagcc acgtcgtgcc gcacggtctg ccccgtcacc
      241 agcgaagcaa gggaaacgaa aatggcgacg cccgcgcttg gcccgtcttt cgggattgct
      301 cccgccggaa cgtgaatgtg catgtcgtgc ccgctaagca acttcgactc ttccggcgca
      361 tcggcaaaac ggctcttcac gaggctcatg gcggcctgcg cgctttctct catgacatcg
      421 ccgagttggc cggtgagtat gagcttgccg ctgccgggca cccgggtgca ctcgatgaac
      481 aggatgtctc cacctaccgg cgtccaggca aggccggtgg cgacacccgg caagccggta
      541 cgcaaggcga cttcattctc gaacttcggg gagccaagaa ttttctccac atcttccgtg
      601 ccgatttgcc tggcctccgc tgcgccttcc gcgaacgtaa ccgcgacgtg ccgcagtact
      661 gcgccaatct gcttctccag gtttcgcacc cctgcctccc gggtatagcc ggtaatgatc
      721 gagagcagcg aatcatcggg gatctccacc tgctccgccg acagcccgtt gttttcgagc
      781 tgccgcgcga cgaggtaccg cttcgcgatc tgcagtttct cctctgttgt gtaacccctc
      841 agttcgatga cctccatgcg atcgagcaag ggcgctgcga tggtatcgag gcggttggcg
      901 gtggcgatga acatgacctt gctcaggtcg aagggcacgc cgagataatt gtcctggaag
      961 gtggtgttct gctcagggtc cagtacttcc agcagcgccg aggcgggatc accgtgcagg
     1021 ctggccccga gcttgtcgat ctcgtcgagc atgaacacgg ggttcctggt gccggccttg
     1081 cgcattccct ggatgatctt cccgggcagt gcaccgatgt aggtgcgccg gtgcccgcgg
     1141 atctcggctt cgtcgtgggt gccgcctaag cttgcgcgca cgaacttcaa tccgaccgcg
     1201 cgggcgatgc tctggccgag cgacgttttg ccgacgcccg gcgggccggc aaagcagaga
     1261 atcgggctgc gtccggacgg gttaagcttg cgcaccgcga ggtattcgat gattcgcttc
     1321 ttgatgtcga cgagaccgta gtggtcttca tcgaggacct gccgtgcttt cgcgatgtcg
     1381 atgctttcgg aatccagttt cgaccacggc agttccgtga gcgagtcgat gtagtttcgc
     1441 agcatcgagt actcgccggc tgcgctcggc atgcgcttca gccgcccgaa ctccttgcgc
     1501 gtatgctcct cgatttcctc gggcatgccg gcgtccacaa tggccttctc gatcgcctcg
     1561 aattccgcgg cattcccttc gtcctcgccg agctgctgct ggatcgtgcg catctgctcc
     1621 cgaagaaccg cctcgcgctg cctctcatcg atcgaagcgc gggtttgctg ctcgatctcg
     1681 tgggacagct cgagaacctc gaagcgcttc gccatcagtt ccagcaccgc atcgaggcgc
     1741 tgcttgatgt cgaatgtctc gagcagccgt tgcttctctg ccgcggggat gtcgaggaag
     1801 ccggcgatga tgtcacagag cattccgggc gagtcaatgg cacgcagtga tgcgcccagc
     1861 tctcccggaa cctgcggcag gagttccagt gctctcgccg cctgttgttt caggacgcga
     1921 acgcgcgcct ctacttcgtc ttccttgccg gtagcctctt ccggtacggc gaagttgccg
     1981 atcaggtgag ggctggtctg gacgaaattc gtgacgcgaa aacgtgtatc tccctggcag
     2041 accaggtggt gagcgttatc cggtgccgta acgtagcgca ggatgttgac cacggtgcca
     2101 acccaataaa ggtcggaagg cgccggggca tccgtttccg cattccgttg cagtagcagg
     2161 cccagcttgc cgttctcccg gatagctgcc tgcgcggcag caatcgatgc aggtcgccgg
     2221 atcgtgacgg gatgaatgac gccggggaag agcaccatgt cccgcacggg cacgatgtgc
     2281 aggcggtcgg atgttgctcc ggtcgggtcg ggcgatccgg aaccaggagc gggttcaggt
     2341 ttgttttcac tcattcgagg aacctcgctg taaccggatt tcgaaacagc cgtcgtggta
     2401 gcggctggcg gcgacccggt atcggccggg cggaagtgca acgcgccgtt cgaggcggcc
     2461 gtaggggatt tcgagctggc ggattccgcc tcgcaccgcc tccgccggga ga
//
LOCUS       DASUOM010000407         6709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1590442,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000407 DASUOM010000000
VERSION     DASUOM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590442"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFY27_08480"
     CDS             complement(<1..331)
                     /locus_tag="VFY27_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765589.1"
                     /translation="MNLNQPLLLLLPFVLAAIAGCEPPPDNAVDSEDMMISKAMDASI
                     YSAAIASEERPEKDIERDERSKPAKVLQFFEIQPGMRVLDLFSGGGYYSELLSRVVGP
                     EGSVVAHN"
     gene            complement(936..2993)
                     /locus_tag="VFY27_08485"
     CDS             complement(936..2993)
                     /locus_tag="VFY27_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765590.1"
                     /translation="MPDVFRPNADAGATLQGRARHCGQRLFIRCLALSLVCVAPLAKS
                     QVTLTEGTNLAADVAPSHGRIAMDLLGSIWIIPQGGGGAEKVSSGLQPAVRPQWSPQG
                     DRILYQTVSVIGSRMWLHDTATSAATTLSDGTDFDQHPSWHPSGERIVFSSAREDTGF
                     DLWEMHLETGLSWRLSHAEGDETEPVWAANGRDLAYIRHQDDQWRLVLRRHGQREQDL
                     LVSGEPLAAPSWRPDGSLLTVLQRSDGEYSLAMVILSDPPLVRTLVEDKDFFLSPVSW
                     ADRMRFFYTADGVIWSRGFNDWKSSRVRFRAEVQRAAPLPATEVETKRLPVVAPSGEE
                     LVIRAARMFDGTRSGYRRNMDVLIRDGRVAAVEPRREWEDVTVLDLGDATILPGFIDS
                     YAALPDADPRVLGIALLSYGVTTVVAEAPENLDPLVWETEQSPGPRLLRAAPLGFAGS
                     VADEGAAPLALQVIPASGIPAAEGQYRAATRIPGLPLLAESWRVGIAVGADLLLGADT
                     MPASPQGNRYQDVLLTVGSNPQTLVSGLADAGTPALPELLQSRQAEAFARFALGREAP
                     PRRLARPPNLAGASSIVVGSKPNALPPGLGLHAELQALQGAGLSGDVLLRAVGANAAD
                     ALGYRELGRIEPGAMADLVLVSGDPGNQILDALNIVAVVRNGRFFSLVRLLEEATSSG
                     IVE"
     gene            complement(2986..3174)
                     /locus_tag="VFY27_08490"
     CDS             complement(2986..3174)
                     /locus_tag="VFY27_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX5765591.1"
                     /translation="MKPAIVERRISCPYCAEYMEVVLDLSAGSQSYIEDCQVCCQPMQ
                     ISFEADEDELHGLQVDRA"
     gene            complement(3186..3881)
                     /locus_tag="VFY27_08495"
     CDS             complement(3186..3881)
                     /locus_tag="VFY27_08495"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEX5765592.1"
                     /translation="MNKLRFADRLPVDLPQDPMPLADAWLREATANARVRNPNAMTLA
                     TAGPDGQPAARVVLCKLFVPDPGYVVFYTNYKSRKGVDLAANPKVGATFYWDSLGLQI
                     RLEGLAVHSPSAESDDYFATRDSGSQLGAWGSDQSREIASHDALVEQIARRAAQLGVS
                     LNGRSQKPDDAAPPLSRPPHWGGFRIWVSRIELWVEGKDRIHDRAAWRRNLVRADAYS
                     FSVSPWKGTRLQP"
     gene            complement(3983..5503)
                     /locus_tag="VFY27_08500"
     CDS             complement(3983..5503)
                     /locus_tag="VFY27_08500"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5765593.1"
                     /translation="MTRVAVATSSQLAADAASEVSRSGGNAVDCALAASLVTMNTQPG
                     VCALAGGAYVTVWAQGSPPVTIDGNVAVPGQSLAPGAAAAGTPVRLGYGGGVDTVVGA
                     GSVAVPGSLAAVEFAWRRYGAVPWAKLLGPSIRAARDGFPLPSACHYYLCYSGTPVFG
                     RSADGYGALHDDGLLRAVGSTVYVPHLADSLEAIASEGSRVFYEGEIASKIAGHIQQG
                     GGALTLDDLRNYEPIVRDCLCVEMNDWHIATNPPPAVGGAVLAAMLLAFRERRMARWD
                     AAALEYLMEVQRAALSWRRQRLDLSEDVAADAARLLELAGGGRLVGTSVSASTVHTSA
                     VDDSGLGCSITASSGYGSGEMPDGTGLWLNNCLGELELNRRGPGALPVGARLSSNMAP
                     TIARSTEGVLSVGTPGADRITTALHQFLVNFLVIGLRLDNAIAHPRMHVDLASGRGRV
                     ALEPGLAFTPDTDLEVALFPEIGMYFGGVGAALYDRNSGFTTGADPRREGATFISS"
     gene            complement(5758..>6709)
                     /locus_tag="VFY27_08505"
     CDS             complement(5758..>6709)
                     /locus_tag="VFY27_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX5765594.1"
                     /translation="GIVPAVLGHEGGAVVEEIGEGVKSVKVGDHVIPLYTPECGVCKF
                     CTSGKTNLCQAIRATQGKGLMPDGTSRFSKNGKPIFHYMGTSTFSEYTVLPEIAVAKV
                     NREAPLEKVCLLGCGITTGIGAVINTAKVEPGSTVAVFGLGGVGLSAIQGAQMAKAGR
                     IIAIDINPSKFELAKQMGATDTVNPKDHDAPIQQVIVDMTDGGVDYSFECIGNVKLMR
                     AALECCHKGWGTSVIVGVAGAGQEICTRPFQLVTGRVWKGTAFGGTKGRSQLPGMVDQ
                     YMNGEIKVDEFITHTMPLEEINKAFDLMHEGKSIRSVVHF"
BASE COUNT         1246 a         2159 c         2078 g         1226 t
ORIGIN      
        1 tgttgtgcgc cacgaccgag ccctcgggac caacgacccg cgaaagcagt tcgctgtagt
       61 agccaccccc ggagaacagg tccagaacac gcatgccggg ctgtatctcg aaaaactgga
      121 ggaccttggc cggcttgctg cgttcatcac gctcgatgtc cttttcgggg cgctcctcgc
      181 tggcgatcgc cgcggagtaa atggaggcgt ccatggcctt ggaaatcatc atgtcctccg
      241 agtcgacagc gttgtcgggg ggaggctcac agccggcaat ggccgcgagg acgaaaggca
      301 gtagcaagag tagcggctga ttcagattca taagtcgttc attctgcaca ggagtccagg
      361 gcgtagtaga ccgtcgcggg tgctcgaagt gcctgccggt gccggatcgc agatcccctg
      421 aagatgcgcg caataaaaaa ccccgcggct gatgaggcga cggggctaat gttttctttt
      481 tttattatta agacgtaact tgagttcggc agccagatac agacatgtca ttattattat
      541 tggatctgga tctttttgtt tttgtagtta ttgttcttct ggcgtctaaa tcctgtttgt
      601 atcgattttc cggctaccgt ttacccagtt acacttcgct tgggcaaact tacaaaagca
      661 agagctatgc cactagtgac ttcttttttt gatttcaaac acttgcccgg aagcgtgccc
      721 agcctgattt aactttgcac gggtggttgt caatttttcc gacatccggc aatgtcgacg
      781 tgacggctag taaaggccga aacgcttaat attcagtgag ttatgcgagg cagcgcggcc
      841 atcactggcc gctgcagggc aatcagagca gtctacgcac cgctggttcc gtcgccaatc
      901 gcagacagtc ccgattcgca ggctgctttg tcaaattatt cgacgatgcc cgaggaggtc
      961 gcttcctcca gcagccgcac caggctgaag aagcgtccgt tgcggaccac ggcgacgata
     1021 ttgagcgcgt ccaggatttg attgcccggg tccccggaca ccagcaccag gtcggccatc
     1081 gcgccaggct ctatcctgcc gagttcccgg tatcccagcg catcggccgc attcgcgcct
     1141 accgcccgca gcagaacatc gccgctaagg ccggcgccct gaagcgcctg taactccgca
     1201 tgcagcccga ggcccggggg cagggcgttc ggtttgctgc cgaccacgat ggaagaagcc
     1261 cctgcaaggt tcggcggtcg cgccaggcga cggggcggcg cttcccggcc cagcgcaaaa
     1321 cgggcgaagg cttcagcctg tcgtgactga agcagttcgg gcaatgccgg cgtgccggca
     1381 tcggcgaggc cggaaacgag cgtctgcgga ttggacccca cggtcaggag gacatcctgg
     1441 taacggttgc cctgcggaga tgccggcatg gtgtctgcac cgagaagcag gtctgcgccg
     1501 acggctatgc caacccgcca gctctccgcg agcaatggca ggcccgggat cctggttgca
     1561 gcgcgatact gtccttcggc cgcagggata cccgatgccg ggatgacctg cagtgccagg
     1621 ggagcggctc cttcatctgc gaccgatccg gcgaagccca gcggagccgc ccgcaacagc
     1681 cgcggaccgg ggctttgctc tgtttcccac actaatgggt ccaggttttc cggcgcttcg
     1741 gcaacgaccg tcgttacgcc atagctcagg agggcgatgc cgagcacgcg cggatccgcg
     1801 tcggggagag cggcgtagct gtcgatgaag ccgggaagga tcgtggcgtc gccgaggtcg
     1861 agcaccgtca catcctccca ttcgcgccgc ggctcgaccg cggcgactcg cccgtccctt
     1921 atcaggacat ccatgtttcg ccggtagccg gaccgggtgc cgtcgaacat ccgggcggcg
     1981 cggatgacga gctcttcccc ggacggagcg acgacaggca gccgcttcgt ttccacctcg
     2041 gtcgccggga gcggagcggc cctctgcact tcggccctga agcgcaccct gctcgacttc
     2101 cagtcgttga agccacggct ccagatgacg ccgtcggccg tatagaagaa acgcatgcga
     2161 tcggcccagc tcaccggcga gaggaagaag tctttatcct ccaccagggt ccgtacgagg
     2221 ggcgggtcgg aaagaatcac catcgccagg ctgtactcgc cgtcgctgcg ctgaagtacg
     2281 gtcagcaggc tgccgtcagg gcgccacgat ggcgccgcca atggctcgcc cgacaccagc
     2341 aggtcctgtt cccgctgccc gtgtcgcctg aggaccagcc gccactggtc gtcctggtgc
     2401 ctgatgtacg caaggtcgcg cccgtttgca gcccataccg gctcggtctc gtcaccttcg
     2461 gcatgactca ggcgccaact gagcccggtt tcgaggtgca tctcccagag gtcgaaaccc
     2521 gtgtcttcgc gagcggatga aaacacgatg cgctcgccgg acgggtgcca ggaggggtgc
     2581 tggtcgaagt cggtgccgtc gctcagtgtg gtggccgcgg aagttgccgt atcgtgcagc
     2641 cacattcggc tgccgatgac cgaaacggtc tggtaaagga tccggtcgcc ctgcggggac
     2701 cattgcggcc ggaccgcagg ctgcaggccg ctactgactt tctcggcgcc gccgccgcct
     2761 tgaggaatga tccagatact gccaagcagg tccattgcga tgcgcccgtg cgagggcgcg
     2821 acatctgccg ccaggttcgt accttccgtc agcgtgacct ggcttttcgc taacggtgcg
     2881 acgcaaacga gcgagagcgc gagacagcga atgaacaggc gctgcccgca gtgtcgcgca
     2941 cgtccctgca gcgtcgctcc cgcgtcggcg ttcggccgga aaacgtcagg cacggtcgac
     3001 ctgcaacccg tgcaattcat cctcgtccgc ctcgaagctg atctgcatcg gctggcagca
     3061 aacctggcag tcctcgatgt acgactggct gccggccgac aggtccagca cgacttccat
     3121 gtattcggcg cagtaggggc agctgatcct gcgttcgacg attgcgggtt tcatcttgtc
     3181 ggtacttatg gttgcagccg tgtccctttc cacggcgaga ccgagaacga gtatgcatcc
     3241 gcccgtacca ggttgcgccg ccacgccgcg cggtcgtgga tcctgtcctt gccctcgacc
     3301 cagagttcaa tccggcttac ccagatgcgg aagccgcccc aatgaggcgg acgtgaaagt
     3361 ggcggcgccg catcgtccgg tttttgcgat cgtccattca gcgacacacc gagctgtgct
     3421 gcacgcctgg ctatctgctc gaccaacgca tcgtgcgatg caatctccct gctttgatcg
     3481 cttccccacg caccgagctg gctgcccgaa tcgcgcgtag cgaaatagtc atcactttca
     3541 gcggagggcg agtgaacagc cagtccctcg agccggattt gcaggccaag cgagtcccag
     3601 tagaaggtgg ctccgacctt tggattggcg gcgaggtcca cacccttgcg cgacttgtag
     3661 ttggtataga acacaacgta ccccgggtcg ggcacgaaca gcttgcacag gacgacgcgg
     3721 gccgccggct gaccgtccgg ccctgcggtt gcgagagtca tggcattggg attgcgaact
     3781 cttgcgttcg ccgttgcctc cctgagccac gcgtcggcga ggggcatcgg atcctgcggc
     3841 aagtcgacgg gcaggcgatc cgcaaagcgc agcttgttca acgactactc cggtaggaat
     3901 accaatcacg ttttcgcgcg cacttgcgtg gcggcaggtc gggagggtag cagacggcga
     3961 ttgcgcgggc atccgcggtt cctcaggatg agataaaggt cgcgccctcg cgccgcggat
     4021 ccgcgcccgt cgtgaagccg ctgttccggt cgtaaagcgc cgcgccgacg ccgccgaagt
     4081 acatgccgat ctccggaaag agcgcgacct cgaggtctgt atccggcgtg aaagcaagac
     4141 ccggttcgag cgcgacacgg ccgcgcccgg aggcaaggtc gacgtgcatc cgcgggtgcg
     4201 caatcgcgtt gtcgaggcgc aggccgatga ccaggaaatt gaccaggaac tgatgcagcg
     4261 cggtcgtaat gcggtcggca ccgggcgtgc cgacggagag aacgccttct gtcgagcgcg
     4321 cgatggtcgg cgccatgttg gatgacaacc gtgccccgac cggcagtgcg ccaggcccac
     4381 ggcgattcag ttccagctcg ccgaggcaat tgttgagcca gagtcccgtg ccgtccggca
     4441 tttcgcccga gccgtagccg gacgatgccg tgatgctgca gccgagcccg gaatcatcaa
     4501 cggccgaggt gtgcaccgtg gacgcggaca cggacgtgcc gaccagcctg ccgcctcccg
     4561 cgagttcaag aaggcgagcg gcatccgcgg caacatcttc actgagatca agtcgctgcc
     4621 ggcgccacga taacgccgct ctttgcacct ccataaggta ttcgagtgcg gcagcgtccc
     4681 agcgcgccat tctccgttcg cggaatgcca gcaacatcgc ggcaagcact gccccgccga
     4741 ccgccggcgg cggattggtg gcgatatgcc agtcgttcat ctcgacgcaa aggcagtcgc
     4801 ggacaattgg ctcgtagttg cgcaggtcat ccagtgtcag cgcgccgcct ccttgctgta
     4861 tatgcccggc aatcttcgag gcaatttcgc cttcgtagaa aacccggctg ccctcgctcg
     4921 ctatcgcctc gagcgaatcc gcaaggtgag gtacataaac cgtgctgccg actgcgcgca
     4981 agaggccatc gtcgtgcagc gccccgtacc cgtccgcgct ccgcccgaac accggggttc
     5041 cggaatagca aagataatag tggcaggcgg acggcagcgg gaagccatcc cgcgctgcgc
     5101 gtatactcgg ccccagcagc ttcgcccagg gcacagcgcc gtatcgtcgc cacgcaaact
     5161 ccaccgcagc aagagacccc ggcacggcca ccgaacccgc cccgaccacg gtgtcgacac
     5221 cgccgccata cccgagccgt accggcgtac cggcagctgc ggcgccgggc gcgagcgact
     5281 gccccggtac ggccacgttg ccgtctatcg tcaccggcgg ggatccttgc gcccacaccg
     5341 tcacgtacgc gccgccagca agggcgcaaa caccgggctg cgtgttcatc gtcaccaagg
     5401 aagccgcgag cgcgcagtcc actgcgttac cgcccgacct ggaaacctcc gaagccgcat
     5461 cggcggcaag ctgcgaggac gtcgcaaccg cgacacgtgt catgtcacag gtctctcacc
     5521 ggcgccgaat gggctgaatg acaccacaca gcatgttcat gaacgcgacg ttcggaaccg
     5581 aagcagctgg ggaggccgtc cctggggaat cgggatgtcg ctacagacct cggaggcatc
     5641 gctggcgggg aggcccttgg cggaaataaa gcggaccgat gggagccatt ctgccaaggg
     5701 cctccccgcc ggcgatgcct aatcgaataa cgaaggggcc agccccaaaa acacaaccta
     5761 gaagtgcact accgaccgaa tgctcttccc ctcgtgcata aggtcaaacg ccttgttgat
     5821 ttcctcgagc ggcatcgtat gcgtaatgaa ctcgtcgacc ttgatctcgc cgttcatgta
     5881 ctggtcgacc atgccgggca actgcgaccg gcccttggtt ccgccgaacg ccgtgccttt
     5941 ccagacgcgg cccgtgacca gctgaaacgg ccgcgtgcag atttcctggc cagcgcccgc
     6001 gacgccgacg ataacggatg tgccccagcc cttgtggcag cactcgagcg cggcacgcat
     6061 gagtttgacg ttgccgatgc attcgaagga gtagtcgacg ccgccgtcgg tcatgtcgac
     6121 gatcacctgc tggatgggcg cgtcatgatc tttcggattg acggtgtccg tggcgcccat
     6181 ctgtttcgcg agctcgaact tcgacgggtt gatgtcgatg gcgatgatgc ggccggcttt
     6241 cgccatctgt gcgccctgga tggcgctcag tcccacgccg cccagaccga agacggcgac
     6301 cgtcgagccg ggttcgacct tggcagtgtt gatcaccgca ccgataccgg tggttatgcc
     6361 gcagcccagg agacaaacct tttcgagcgg cgcctcccgg ttgaccttgg cgaccgcgat
     6421 ctccggcaac acggtgtatt cgctgaatgt cgaggttccc atgtagtgaa agatcggctt
     6481 gccgttcttg gaaaaccgcg aagtgccgtc gggcatcagg cccttgcctt gcgttgcgcg
     6541 aatcgcctgg cacaggttgg tcttgccgga agtgcagaat ttgcacacgc cgcactcggg
     6601 cgtgtaaagc gggatcacgt gatcgccgac cttgacgctc ttgactcctt ccccgatctc
     6661 ttccacgacc gcgccacctt catggccaag gacggcaggg acaatacct
//
LOCUS       DASUOM010000408         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1337649,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000408 DASUOM010000000
VERSION     DASUOM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337649"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFY27_08510"
     CDS             complement(<1..432)
                     /locus_tag="VFY27_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765595.1"
                     /translation="MSITTAVFSSVLLLSFAAGAAACFFIRNVSDYYVAGGRMPWYLL
                     TGTFLASNVSAGLFLGATNMAGTNGYSIWSSYVPTSIGFLIAIAFVGVMVRRLSRHYE
                     VYDFADILAARYSSRSVHVRTLSAVLLAVVYIPILGAQFIAL"
     gene            complement(974..1615)
                     /locus_tag="VFY27_08515"
     CDS             complement(974..1615)
                     /locus_tag="VFY27_08515"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5765596.1"
                     /translation="MDTTAGTAHIRTRKGTALRRRGLARVRKILASSEKVFVNQGYQN
                     FSLRNVAVETGISLGNLTYYFQSKEDLLRSMIDGILADYESGLDAILKEFPGDPEARL
                     RGYMQYLLADSRHPERHQFFYHFWAISTHDRFVAGCRRRAYAAFRDQVKGLCRAVNPA
                     LSEKTVTQRTYLLMALIEGMHVLFGNRKTPDENLDKIAHEFCKQAMGIVTSSE"
     gene            1764..>3878
                     /locus_tag="VFY27_08520"
     CDS             1764..>3878
                     /locus_tag="VFY27_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765597.1"
                     /translation="MQHRNKTVRSGVTLTVASLLLPVNSIAQVLEEVVVIAQKREENL
                     QDVGISVTAFSGDQLESLGVTSTADITQQVPAMQLFTYTPAFTVFSLRGISQNNFQDN
                     LEAPVAVYIDRVYVATMNAINAQLYDVERVEVLRGPQGTLFGRNATGGLVHYLTRKAT
                     EDELNGYVQATAADFDTYSVEGAVGGAFSEAVRGRLAGRWETSEGYVEAGSAFGVQAT
                     GRTSHGADGYSVRGTLQISAGDDVTIDLTGAYSKDDDAPSGQYVVALAGFDPDTGLGA
                     FTDAFNLDPVDPNNNPPVGPVDYDGAPITGDVHRHWSNESNTFFDRDVASLTAQVTAS
                     IGAAELVSITNYTSMDKEYLEDAGGGFGFFPYNTLNDYDQLSQEFHLSGGSERWRWQV
                     GAYYLDMTWDTFQAVEGALIHGPGFTDAARTETFGKVDSRNWSVFGQAELDFAPRWTG
                     ILGLRWSQDDKELDYTRYFSDAGIGVERTLTFDIANDPTPDIDTIDYGDYALRAQLNW
                     TPADSLLFYGSYSRGIKGGNWSLDPLGGVPGFNLKHGEEVLNSYELGIKSELADGFAR
                     LNAAAFYYDYEDYQAFNILNVVPQVTNSDATASGGEVELVMLPMEGLELMFGASVIDS
                     EVDAVTDVFGGTLTAEFPNAPGFSLNWLARYEWPALGGSLATQIDGFYNDNQYLEGTN
                     SEVSFEGAYAVWNARFFYLTGNE"
BASE COUNT          850 a         1110 c         1152 g          766 t
ORIGIN      
        1 caatgcgata aattgggcgc cgagaatggg aatatatacg accgccagca acacggcgct
       61 caacgtgcgc acgtgtacac tgcggctgct gtaccgcgcc gccaggatat ccgcgaagtc
      121 gtagacctcg tagtggcggg aaagccgtcg aaccatcacg ccgacgaagg caatggcgat
      181 caggaagccg atgctggtgg gaacgtagga actccagatt gagtacccgt tggtaccggc
      241 catgttggtc gcgccaagaa aaagaccggc actgacattg gaagccagaa aggtgcccgt
      301 aagcaggtac cagggcatcc gtccacctgc gacgtagtag tcagatacat tgcggataaa
      361 gaagcacgcc gccgctccgg cagcgaaact cagtagcaaa acggaagaaa agacggctgt
      421 agtgatgctc atcgctggtg acgacggcgc ataagccaag gctttccggc gataaccgct
      481 cgccagtata tccagcagga ggcagcaacg acggtgagcc agaagaccaa cccgatccgc
      541 agaaccatgt caaggcgtga ccagggaaaa taccatccaa caaagtcgag catcagacgg
      601 tcacccagag aggtttccaa tcgctataac tgaaggcgag ttcccgcccg gcgtggcctc
      661 gaaatccgcg cgaaatccgg cccgccacac ccacgcgtga aatgtggcac gtttccagcg
      721 gtaactccgt tccgactgtc gaagctgact caaagcttcg accagcccga ggtggtcacc
      781 cgcgaagccc gcttggccgc cgctcatatt gtgcggtagt tcgctgttag cggacatagg
      841 aaatgaccgt tttcggacaa atgatcgacc cctctttgta taaccagggc cgacaggttg
      901 aaggggaggc ccgacccttg cacagggagg gatcatagag gttcgcatac gcgaggcagc
      961 tgaagccgga gagctattca gaggaagtca cgatccccat agcctgcttg cagaattcat
     1021 gtgcgatctt atcgaggttt tcatcgggtg ttttgcggtt accaaacagg acgtgcatgc
     1081 cctcgatgag ggccatgagg aggtaggtcc gttgggtgac ggttttttcc gatagagccg
     1141 gattaacggc tcggcacaag cctttcacct ggtcgcgaaa agccgcgtac gctcgccgcc
     1201 ggcaaccggc cacgaatcga tcatgcgtag agatagccca gaagtgataa aagaactggt
     1261 gccgttcggg atgccgcgag tccgccagca ggtactgcat atagcctcgg agtctggctt
     1321 caggatcgcc cggaaactcc ttcaggattg cgtccaggcc gctttcatag tcggcgagta
     1381 tgccgtcaat catcgacctg agcaggtcct ccttcgattg gaaatagtag gtcaggttgc
     1441 cgagactaat gccggtctcg actgcaacat tacgcaagga aaagttctga tatccttgat
     1501 ttacaaaaac tttctcagag gatgccaata tttttcgaac gcgtgccagt ccccgccgcc
     1561 gcagcgccgt tcccttgcga gtccgaatgt gggctgtgcc ggcagtcgta tccatcactt
     1621 atccagaatt tggctcggag cggatcgtcc gctccacgac cgagtatgac tcgcacagca
     1681 cggcttgcaa agtaggaacg gttgtcctat aaagtggcgg ataccacatg gcgaggtgcg
     1741 ttgcactttg ggggacatca accatgcagc accgtaataa aaccgtgcgc tccggcgtga
     1801 cgttgacagt cgcgtcgctg cttctgcccg ttaatagtat tgctcaggtc ctcgaagagg
     1861 ttgtcgtcat cgcgcagaag cgtgaggaga acctgcagga cgtcggcatc tcggtgaccg
     1921 ccttttcggg cgatcagctc gagagtctcg gggtgacgag caccgccgat atcacccagc
     1981 aagtcccggc gatgcagtta tttacctaca cgccggcatt taccgttttc agtttgcgcg
     2041 ggatttcgca gaacaacttc caggacaacc tggaggcgcc ggtcgctgtt tacatagacc
     2101 gtgtctacgt agcgaccatg aacgccatca atgcccagct ctacgatgtc gagcgagttg
     2161 aagtgctacg cggaccacaa ggaacactgt tcggccgcaa cgcgaccggt gggctggttc
     2221 actatctgac gcgaaaggcg acggaagacg agttgaacgg ctatgttcag gcgaccgccg
     2281 ccgatttcga cacttacagc gtcgagggcg ccgtgggcgg cgccttcagc gaagccgtcc
     2341 gcggccggct ggcgggcagg tgggagacgt ccgaaggcta tgtggaagct ggatcggcgt
     2401 tcggtgtgca agcgaccggc cgcacgtcgc acggcgcaga cggctattcc gtgaggggga
     2461 cgctgcagat aagcgcaggc gatgacgtta ccatcgattt gaccggcgcg tattcaaagg
     2521 acgacgacgc accgtccggg cagtatgtcg tggccctcgc gggtttcgat ccggacacgg
     2581 gtctcggagc ttttaccgac gctttcaacc tcgatccggt cgacccgaac aacaatccac
     2641 cggtcggtcc cgtcgattac gacggagcac cgattaccgg cgacgtgcac cgccactggt
     2701 cgaacgagag caatacgttt ttcgatcgag acgttgcttc cctgacggcg caggtgacgg
     2761 cttcgattgg cgcagcagag ctcgtttcca tcaccaacta cacctcgatg gacaaggagt
     2821 acctggaaga cgccggcggc ggattcggct ttttcccgta caacacgctc aacgactacg
     2881 accagctgtc acaggaattc catttatccg gcggcagcga gcgctggcgc tggcaggtcg
     2941 gtgcgtatta cctggacatg acctgggaca cctttcaggc tgtggagggc gctctcatcc
     3001 acgggccggg atttacggac gccgcgcgca cggaaacctt cggcaaggtc gactcccgca
     3061 actggtcggt tttcggtcaa gccgagctcg acttcgcgcc tcgatggacc gggatcctcg
     3121 gccttcgctg gtcccaggac gataaggagc tcgactacac gcgttatttt tccgacgcgg
     3181 gtatcggagt cgagcgcacg ttgacgttcg atattgcaaa cgacccgact ccggacatcg
     3241 atacgatcga ctatggcgac tacgcgctgc gtgcccagct caactggacg cccgccgaca
     3301 gcctgctgtt ctatggatcg tatagccgcg ggataaaagg cggtaactgg tcgctcgatc
     3361 cacttggagg agttccgggc ttcaacctga aacatgggga ggaggttttg aactcctacg
     3421 agctggggat caagtcggag ttggccgacg gttttgcgcg cctgaatgct gctgcttttt
     3481 attacgacta cgaagactac caggcattca acatcctcaa tgtcgtgccg caggtcacca
     3541 actccgatgc gaccgcctcc ggcggtgagg tcgagctggt aatgctgccg atggaaggct
     3601 tggagctgat gttcggcgcg tctgtgatcg attcggaagt agatgccgtg accgacgtct
     3661 tcggcggtac tctcacggct gagttcccga atgcacccgg cttcagcctc aattggctgg
     3721 cgcgttacga gtggccggcg ctcggcggct cgctggccac acaaattgac ggcttctaca
     3781 acgacaacca gtatctcgag ggaaccaact ccgaagtctc ctttgagggc gcttacgccg
     3841 tctggaacgc ccggtttttc tatctcaccg gcaacgag
//
LOCUS       DASUOM010000409         4864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_649208,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000409 DASUOM010000000
VERSION     DASUOM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649208"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..538)
                     /locus_tag="VFY27_08525"
     CDS             complement(56..538)
                     /locus_tag="VFY27_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5765598.1"
                     /translation="MKRNKSRTCALPGALLIVGLAMAGAVPASDIYKWTDAEGNVHFG
                     DRPSGASTEERLAINSTPTDPDRVRDIVQARTDSRTERSVAEAAAKAAEPTAKEIRAA
                     EAERKEKCNKYKDRLQKFVTSRRLYRQDENGERVYLDEEETLAARARVQEQVLEYCNP
                     "
     gene            complement(740..2023)
                     /locus_tag="VFY27_08530"
     CDS             complement(740..2023)
                     /locus_tag="VFY27_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5765599.1"
                     /translation="MEDVTQLAGQLAELSYALDTFYFLVMGALVMWMAAGFTMLESGL
                     VRAKNTAEILTKNVGLYAIACIMYMLCGYSIMYPGAYEGGFLQSLSSFGTGLVGTADN
                     EAADVIASGGETYYSSLADFFFQIVFVATAMSIVSGAVAERMKLWSFFLFAVVMTSII
                     YPVQGFWKWGGGFLDTAGFLDFAGSGVVHMTGAAAALAGVLLLGARKGKYGPGRQVNA
                     IPGCNLPLATLGTLILWFGWFGFNGGSELKVSNVAEANAVSLVFVNTNLAAAGGLVFA
                     LILSRLRFGKADLTMALNGALAGLVSITAEPLTPSPIVATLIGAAGGVLVVLSIVTLD
                     KLRIDDPVGAISVHGTAGIWGVLAVPITNDGASVAAQLLGLVSIFAWVFLTSFIVWFI
                     LKKTVGIRVDEQQEYEGVDLSECGLEAYPEFTKAD"
     gene            complement(2038..2376)
                     /locus_tag="VFY27_08535"
     CDS             complement(2038..2376)
                     /locus_tag="VFY27_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5765600.1"
                     /translation="MKLIMAIIKPFKLDDVRQAVSDIGIQGITVTEVKGFGRQRGHTE
                     LYRGAEYVVDFLPKAKIELAVADDVAEQVIEAITNMARTGAIGDGKIFVVDLEQAIRI
                     RTGETGAAAV"
     gene            complement(2401..3021)
                     /locus_tag="VFY27_08540"
     CDS             complement(2401..3021)
                     /locus_tag="VFY27_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX5765601.1"
                     /translation="MKTIKRLLVSAAFLSWSGLALADISADIGWASDYFYRGILQAPT
                     SASAGIDFEKNGFYVGTWAADVKDGLEVDGYFGYSTEIENISYSVGFTGYYYTGDFDD
                     TYQEINLGLGYGYVALDVAIGEHDNFGGGTQDYTYYALTVANKGLYGKFAGFSQDFEG
                     EYLELGYGTTVAELDIGISAIFANSDLAGDPDESLVFTIGKSFELK"
     gene            3282..3581
                     /locus_tag="VFY27_08545"
     CDS             3282..3581
                     /locus_tag="VFY27_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5765602.1"
                     /translation="METIDRLARRLAEAVPEGLRSARDDLEQNFRSVLHSGISRLDLV
                     TREEFDVQQAVLARTREKLEALERRLGTMEAATAAGTAGAAAGNEAAPGTEDTGA"
     gene            3707..>4864
                     /locus_tag="VFY27_08550"
     CDS             3707..>4864
                     /locus_tag="VFY27_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5765603.1"
                     /translation="MLKSRAQVGIDAPEVTVEVFLSGGLPSNSIVGLAETAVRESKER
                     VRGAILNSNFKFPQERISVSLGPADLRKSGGRFDLAIALGILAAQRIVPQHDLDEYEF
                     YGELGMNGELHPIPGALPAAIKAGEAGKAVFVPLRNGLEATLSTARVFVAESLLEVTG
                     HLRGTRMLAPLHRCIPPVVSATAADLADVKGQGVARRALEIAAAGGHNILFSGPPGTG
                     KSMLARRLPGILPPMSDREALETAAVDSVLGRTFEFSRWRQRPFRAPHHTASAAALVG
                     GGSDPRPGEISRAHNGVLFLDELPEFSRHVLEVLREPMESGSTTISRAVRQADFPARF
                     QLVAAMNPCPCGYLGDPKSDCRCSEDRVRNYRAKISGPLLDRIDIQVVVQRL"
BASE COUNT          907 a         1520 c         1507 g          930 t
ORIGIN      
        1 gttgccggct cctgccgacg tgggctccta cacggaagcg cccgaacgct cctccctaag
       61 ggttgcagta ctccaggacc tgttcctgga cacgggctcg ggccgccagt gtttcttcct
      121 cgtcgagata gacgcgctcg ccgttttcgt cctgccggta caggcgccgg gaggtaacga
      181 atttctgcag cctgtcttta tacttgttgc acttttcctt gcgttcagcc tccgcggcgc
      241 ggatctcttt cgccgtgggc tcggctgcct tcgctgccgc ttctgctacg ctgcgctcgg
      301 tcctggagtc ggtccgcgcc tgcacgatgt cgcgcacccg gtcgggatcg gtgggagtcg
      361 agttgatcgc gaggcgctcc tccgtcgagg cgccggacgg gcggtcgccg aaatgcacgt
      421 tgccttcggc atcggtccac ttgtagatgt cgctggccgg gacggcgcct gccatagcca
      481 gtccgactat cagcagtgcg cccggcagtg cgcacgttct tgatttgtta cgcttcatcg
      541 caatcctgga gattcgcgcg ctgcacaaac ttgtaaggca ttaaagcacg ttcacggtag
      601 acgtagtgtg accctgatca cagccctttt tgtcctcgat acgcgcaaaa aaagcgggcc
      661 atgaaatcat ggcccgcttc ggcagggccg ccgagggggc gacaggcccg gcacttgctc
      721 gtttgcgtta atctttctac taatcggcct tggtgaactc ggggtaggct tcgaggccgc
      781 actcgctcag gtctacgcct tcgtattcct gctgctcgtc gacccttatt cccacggttt
      841 tcttcagaat gaaccagacg atgaagctgg tgaggaacac ccaggcgaaa atcgacacca
      901 ggcccaacag ctgtgccgcg acagaggcac cgtcgttggt gatcggtacg gccaacacac
      961 cccaaatgcc cgccgtcccg tgcacggaaa tggcaccgac aggatcgtcg atccggagct
     1021 tgtcgagggt cacgatcgaa agaacgacga ggacaccgcc tgcggcgccg atgagcgtcg
     1081 caacgatcgg cgagggcgtc agcggctccg ccgtgatcga caccagcccg gccagcgcgc
     1141 cgttcagggc catggtgagg tcggccttgc caaagcgcag ccgcgacagg atcagcgcga
     1201 ataccaggcc ccccgctgcg gccaggttgg tattgacgaa tacgagggat acggcattcg
     1261 cttcggccac gttcgatacc ttcagctccg aaccgccgtt gaagccgaac cagccgaacc
     1321 agagtatcag cgtgcctaac gttgcaagtg gcaggttgca tcccggaatt gcgttgacct
     1381 ggcgtcccgg gccgtacttg cctttgcggg cgccgagcag cagcacgccg gcaagcgccg
     1441 ccgccgcgcc cgtcatgtga accacgcccg aacctgcaaa atccaggaaa ccggcggtgt
     1501 cgaggaagcc tccgccccat ttccagaagc cctgcaccgg gtagatgatc gaggtcatca
     1561 ccacagcaaa gaggaagaag gaccagagct tcattcgctc cgcaaccgcg cccgatacaa
     1621 tggacatcgc cgtcgcaacg aacacgatct ggaaaaagaa atccgcaagg ctggagtagt
     1681 aggtctcgcc gccgctggcg atcacgtccg ccgcctcgtt atcggcggta cccacgagcc
     1741 cggtgccgaa actcgagagg ctctgtagga aacctccttc gtatgcgccc gggtacatga
     1801 tcgagtaacc gcacagcatg tacatgatgc acgcgatagc atatagcccg acgttcttcg
     1861 tcagtatttc ggcggtgttc ttggcacgaa cgaggcccga ctctagcatg gtgaagccgg
     1921 cggccatcca catgacgagt gcgcccatga ccaggaagta gaaagtatcc aatgcatagc
     1981 tgagttcggc cagctgcccg gcaagctgcg tgacatcttc cacggctgtt ctccttgtta
     2041 gaccgcggcg gcgccggttt caccggtgcg aatgcgaatg gcctgttcca ggtcgactac
     2101 gaagattttt ccgtcgccga tagcgccggt tcgtgccata ttggtgatcg cttcgatgac
     2161 ctgctccgcg acatcgtcgg cgaccgccag ctcgatcttt gctttcggca ggaagtccac
     2221 gacgtactcc gcgccacggt aaagctcggt atggcctcgc tggcggccga accccttgac
     2281 ctccgtcacc gtgatgcctt gtatgcctat atccgagacg gcctgccgca cgtcgtccag
     2341 cttgaatggc ttgatgatcg ccattatcag tttcatcgtt gtttaccctg cagtgagttt
     2401 ttatttgagt tcgaaggact tgccgatcgt gaaaaccagc gactcgtccg gatccccggc
     2461 gaggtcgctg ttcgcgaata tcgccgagat cccgatatcc agttccgcca cggtcgtgcc
     2521 gtagccgagt tccaggtact caccctcgaa gtcctgtgag aagcccgcga acttgccgta
     2581 gagacccttg ttcgccaccg tcagtgcgta gtacgtgtag tcctgcgtgc caccgccgaa
     2641 gttgtcgtgc tcgccgatcg ccacgtcgag tgctacatag ccgtacccga ggccgaggtt
     2701 tatttcctgg taggtgtcgt cgaagtcgcc ggtgtagtag tagccggtga agccgacgct
     2761 gtagctgatg ttctcaatct cggtcgagta gccgaagtac ccatcgactt caagtccatc
     2821 ttttacgtcg gccgcccagg taccgacgta gaagccgttt ttctcgaagt cgataccggc
     2881 ggatgccgaa gtgggagcct gcaggatacc gcgatagaaa tagtcgctgg cccagccgat
     2941 gtctgcggat atgtccgcca gcgcgaggcc ggaccaggac aagaacgctg cggataccaa
     3001 gagtctcttt atcgttttca ttccactccc catcaagttg taggcggcca ctggtggagc
     3061 agcacgcctg ccccggcgga cccggttttg caacatgcgt gccagtggca gaagtggcgc
     3121 aaaaccggcg ctaaagcgca aagagcgcag gcccatggca ccgatgcagt gcagaggcag
     3181 cggcgattgc tccaatgcgg tgagtgtgtg aagagaatcg ggcagctatg acgcgatttc
     3241 aggactggtc ttttcgccac ggttgcggta tctagagacg catggaaacg atcgacaggc
     3301 ttgctcgcag actcgcggaa gcggtgccgg aaggcctgcg gtccgcgcgc gatgacctcg
     3361 aacagaactt ccgctccgtg ctgcactccg gcatttcgcg actcgacctg gtgacgcgag
     3421 aggaattcga cgtgcaacag gcagtgctcg cgcgcacgcg cgagaagctc gaagcacttg
     3481 aacggcggct cggtacgatg gaggcagcga ccgccgccgg tacggctgga gccgcggcag
     3541 gcaacgaggc ggcgccgggc accgaagaca ccggggcctg atcacaggcg acttctccgg
     3601 aaacggtcat tgccatggag gggcgatgga cgacgggtct gcaaaatccg tgttcgacac
     3661 ggaaggcgag gccggcgggc gcgaggtgag cccggtcagc ctggcgatgc tgaaaagccg
     3721 cgcgcaggtt ggcatagatg ctcccgaggt aaccgtagag gtcttcctgt ccggagggct
     3781 gccctcgaac tcgattgtcg ggctcgccga aacggccgtg cgggaaagca aggagagggt
     3841 gcgcggcgcg atcctgaact caaacttcaa gtttccacag gaacgtatca gtgtcagctt
     3901 agggcctgcc gaccttcgca agagcggcgg ccgcttcgat ctcgcgatag ccttgggcat
     3961 cctcgccgcc cagcgcatcg tcccgcagca cgatctcgac gagtacgagt tctacggcga
     4021 actcggcatg aacggcgagc tgcacccgat acctggcgcg cttcccgcgg ctatcaaggc
     4081 gggcgaggcc ggcaaagcgg tcttcgtgcc gctcaggaac gggttggagg cgacgctctc
     4141 cactgcccgc gtcttcgtcg ccgaatcgct gctggaggtc accggccacc ttcgcggcac
     4201 ccggatgctg gcacccctgc atcgctgcat tccgcccgtc gtttcagcca cggcagccga
     4261 tctcgctgac gtgaagggac agggcgtggc gaggcgcgcg ctggagatcg ccgctgccgg
     4321 cggccataac atccttttca gcggtcctcc cggtaccggc aagagcatgc tggcgcgtcg
     4381 cctacccgga atcctgccgc cgatgagcga tcgcgaggcg ttggaaacgg ccgcggtcga
     4441 ttccgtgctc gggcgcactt tcgaattctc gcgatggcgg cagcgcccgt ttcgcgcgcc
     4501 gcatcacacg gcttcggcgg cggccctcgt cggcggtggt tcggatccgc gacccggcga
     4561 aatctcgagg gcgcacaacg gcgtgctgtt cctggacgaa cttccggaat tcagccgcca
     4621 tgtactggaa gtccttcgcg aaccgatgga gagcggcagc acgacgatct cccgggccgt
     4681 tcggcaggcg gactttcccg cgcgcttcca gctggtggcg gcgatgaacc cgtgtccttg
     4741 tggctatctc ggggatccga agagcgactg ccgctgttcg gaggaccgcg tgcgcaatta
     4801 ccgggcgaag atctccgggc cgctgctgga ccgtatcgac atccaggtgg tggttcagcg
     4861 gcta
//
LOCUS       DASUOM010000410         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1003835,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000410 DASUOM010000000
VERSION     DASUOM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003835"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="kynU"
                     /locus_tag="VFY27_08555"
     CDS             complement(<1..851)
                     /gene="kynU"
                     /locus_tag="VFY27_08555"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX5765604.1"
                     /translation="MTGDDFQPGREFASSLDASDELRAFRERFNFPDSVQGLRPVYLC
                     GHSLGLQPKAAVRYVQEELDDWARYGVEGHFQARRPWLPYHRFATSHLGELAGARDEE
                     VVAMNTLTVNLHVLMTTFYRPTKSRYKILIESTAFPSDRFAAMSQIRLHGFAPPEALV
                     EWRPRETDQALHVEDLQQILDECGDRIALLLLPGVQYLNGQLLDMKDLTARARAKGCC
                     IGFDLAHAMGNVPLELNDWGPDFAAWCSYKYLNGGPGAVGGAFVHSRHLDGDATKQLL
                     GWWGHAE"
     gene            complement(848..1858)
                     /locus_tag="VFY27_08560"
     CDS             complement(848..1858)
                     /locus_tag="VFY27_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5765605.1"
                     /translation="MLKIDIHTHILPRDWPDLAERYGYGGFVQLEHHGPACARMMIDG
                     KEFREIEANCWDPAVRLADCDSTSVNVQVLSTVPVMFSYWAKAEHTADLARILNDHIA
                     SLVDEYPRRFVGLGTLPMQDAAMSITELERCCGPLGLAGVQIGSHVNGWNLDDERIFP
                     VLEAAADLGAAVFVHPWQMLGRERMGLYWLPWLVGMPAETALSICSLIFGGVFERLPK
                     LRVAFAHGGGAFPGIVGRLEHGFRVRPDIVATRNERNPREYLGRFYVDSLVHEPAMLR
                     YMIELMGAECIALGSDYPFPLGEQYPGAMIESMNLDPQTRDTLLAGAALTWLNLERNR
                     FS"
     gene            complement(1883..2401)
                     /locus_tag="VFY27_08565"
     CDS             complement(1883..2401)
                     /locus_tag="VFY27_08565"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX5765606.1"
                     /translation="MQSLTAFNFRKWIDEHRHLLKPPVCNQQVFDDDDFIVMVVGGPN
                     SRKDYHYDEGPEFFYQLEGDMLLKTMQDGSRVDIPIREGEILLLPPKVPHSPQRFADT
                     VGLVVERKRLPEELDGFMWFCDNCGHKLYEEYLHVTDIVGQLPPVFDRFYGSGENRTC
                     GNCGAIMPLPGK"
     gene            complement(2532..2957)
                     /locus_tag="VFY27_08570"
     CDS             complement(2532..2957)
                     /locus_tag="VFY27_08570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5765607.1"
                     /translation="MRGKNSIFASGAASPVGPYPHARRAGDFLFLSGIGPRPADGGSV
                     PGTTFKADGSVLDYDIEMQCHAVFRNVRAVLDSAGATFDDLVDITVFLTNIERDFETF
                     NRVYAEYFPGEGPCRTTVEVNRLPTPIAIELKCIAFVAL"
     gene            complement(3043..>3677)
                     /locus_tag="VFY27_08575"
     CDS             complement(3043..>3677)
                     /locus_tag="VFY27_08575"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX5765608.1"
                     /translation="VLGNHDLHLVAVANGFGAPGERKGSLAKLLRADDCEELIDWLRM
                     RPLAHFSESLNTLMVHAGLHPRWTVKKALARAAEVEHVLRGSEYRQLLPKLYGNSPSR
                     WSGKLTGNKRLRFIVNCLTRIRMIDPDGRLDYEHKGPPANAAKGLVPWFDAPDARWRG
                     TRIVFGHWSALGLVVKPDVIGVDTGCVWNRELTAVRLTKRAKIIQVGCSK"
BASE COUNT          695 a         1171 c         1122 g          689 t
ORIGIN      
        1 tcctccgcat gaccccacca cccgagaagt tgtttcgtgg cgtcgccgtc gagatgccgt
       61 gagtgcacga acgcaccccc aacggcgccg ggccctccgt tcaggtactt gtagctgcac
      121 catgccgcaa aatccggacc ccagtcattg agttcgagcg gcacgtttcc catcgcatgt
      181 gcgagatcga accctatgca gcagcctttt gcacgggcac gggcggtcag gtctttcata
      241 tcgagtagct ggccgttcag gtactgcacg ccgggcagaa gcagcagcgc aatccggtcg
      301 ccgcactcgt cgagaatctg ctgaaggtcc tccacatgca gtgcctggtc cgtttcccgg
      361 ggcctccatt caaccagcgc ctccggcggc gcaaaaccgt gcaggcgaat ctgcgacatc
      421 gcggcgaagc ggtccgacgg gaacgccgtc gattcgatca ggatcttgta ccgggatttc
      481 gtcggccggt agaacgtcgt catcaggacg tgcaggttga ccgtcagtgt gttcatcgcg
      541 acgacttcct cgtcacgagc accggccagt tcacccaggt gtgaagtggc aaaccggtgg
      601 tacggcagcc acggccggcg cgcctggaaa tgtccctcga cgccataccg cgcccaatcg
      661 tccagttctt cctgcacgta acgaaccgca gccttgggct gcaggccgag agagtgcccg
      721 cagaggtaaa ccggccggag tccttgcacc gagtccggga aattgaagcg ctctcgaaat
      781 gcgcgcagtt cgtccgaagc gtccagggat gatgcgaatt cccggcccgg ctggaaatca
      841 tcccccgtca cgagaagcgg ttcctctcga ggttcagcca ggtcagcgcc gcgccagcca
      901 gaagcgtgtc gcgcgtttgc gggtccaggt tcatggactc gatcatggcg cccggatact
      961 gctcgcccag agggaacggg tagtcgctgc caagcgcaat gcactccgcg cccatcagtt
     1021 cgatcatata cctgagcatg gctggttcgt gcaccaggga gtcgacatag aaacggccga
     1081 gatactcgcg agggttgcgt tcgttgcgcg tggcaacgat atccggacgc acgcggaagc
     1141 catgctccag ccgtcctacg ataccgggaa acgcgcctcc gccgtgggca aatgcgacgc
     1201 ggagttttgg cagcctttcg aatactccgc cgaagatcaa tgagcaaatg gacaaggccg
     1261 tctccgccgg catcccgacg agccacggca gccagtaaag tcccatccgt tcgcgtccca
     1321 gcatctgcca gggatgcacg aagacggcgg caccaaggtc cgcggctgcc tcgagaacgg
     1381 ggaatattcg ctcatcgtcg aggttccagc cgttgacgtg cgagcctatc tgcacacccg
     1441 cgagccccag cgggccgcag catctctcga gctcggtaat cgacattgca gcgtcctgca
     1501 tcggcagcgt gccgaggccg acgaaacggc gcgggtactc gtcaaccagg gaggcgatat
     1561 gatcgttcag aattcgcgcg aggtcggcgg tatgctcggc cttcgcccag tagctgaaca
     1621 tcaccggaac ggtcgagagc acctgcacgt tcacgctggt cgagtcgcag tcggccaggc
     1681 gtaccgccgg gtcccagcag tttgcttcga tctcgcgaaa ctccttgccg tcgatcatca
     1741 ttcgcgcaca agcgggacca tggtgctcga gctgcacgaa tccgccgtac ccgtatcgtt
     1801 ccgccaggtc tggccagtcg cgcggaagga tgtgcgtgtg tatgtctatc ttcagcatga
     1861 ccggccgcgg cgtcggtggg acttacttgc caggcagcgg catgatcgcg ccgcagttgc
     1921 cgcaggtgcg gttctccccg gagccgtaga agcgatcgaa aaccggcggc aactggccga
     1981 cgatgtcggt tacgtgaagg tattcctcgt acagtttgtg gccgcagttg tcgcagaacc
     2041 acatgaagcc atcgagttct tccggcaggc gtttgcgctc gactacgagg cctacggtgt
     2101 cggcaaaacg ttgcggtgag tgcgggacct tcggtggcag cagcaggatc tcgccttcgc
     2161 gtatggggat atcaacccgg ctgccgtcct gcatcgtctt cagcagcata tcgccttcga
     2221 gctggtaaaa gaactccggc ccttcatcgt aatggtagtc cttgcgcgaa ttcgggccgc
     2281 cgaccaccat gacgatgaaa tcgtcgtcgt cgaacacctg ctggttgcag accggcggct
     2341 tcaacaggtg gcgatgctcg tcaatccact tccggaaatt gaacgctgtc agcgattgca
     2401 tggtcctgcc tccatccggc gtcgccttcg gctgtggtcg caggaaccct caagggcttt
     2461 gaggccctgc aggagcccga tacgcgggcg agtgcgattc ggtgcagcga accggccggt
     2521 gtggacggct cctacaatgc tacgaaggcg atgcatttca gttcgatggc aatgggcgtc
     2581 ggcaaccggt tgacttcaac cgtcgtgcgg caaggccctt cgccaggaaa gtattctgca
     2641 taaacacgat tgaaggtctc gaagtcccgc tcgatgttcg tcaggaagac cgtgatgtcg
     2701 acgaggtcgt cgaaagtagc gcctgcgctg tcgagtacgg ctcgcacgtt gcggaaaacc
     2761 gcgtggcact gcatctcgat gtcgtaatcg agcaccgatc cgtccgcttt gaacgttgtg
     2821 ccagggacgg agccaccatc tgcaggacgc gggcctatcc ccgacaggaa caggaaatcg
     2881 cctgccctgc gcgcgtgtgg gtaaggccct accggcgaag cggcgccgct cgcgaaaatg
     2941 ctgttcttgc ccctcatcgg cattgtcccg atcgtgagcc gcgcagtgta ccccgctgcg
     3001 ggccaacagg catccgggcg cgccttggca ctattgccgc catcacttcg agcagccaac
     3061 ctggatgatc tttgcgcgtt ttgtcaggcg caccgcggtg agttcccgat tccacacgca
     3121 cccggtgtcc acaccgataa catcgggctt caccaccagc ccgagagccg accagtgccc
     3181 gaacacgatc cgcgtgccgc gccagcgcgc atcgggagcg tcaaaccatg gaaccaggcc
     3241 cttcgctgcg ttggccggcg ggcccttgtg ctcgtagtcg aggcgtccgt ctggatcgat
     3301 catgcggatg cgggtcaggc aattcacgat gaagcgcagg cgcttgttgc ccgtgagctt
     3361 gccggaccag cgcgaggggg aattgccgta gagcttcggc aggagttgcc ggtattcgct
     3421 gcccctcagt acgtgttcga cttcagccgc gcgagccagc gcctttttca cggtccatcg
     3481 cggatgcaaa ccggcatgca ccatcagcgt gttcagcgac tcgctgaagt gcgcgagggg
     3541 ccgcatacgc agccagtcga tcaactcctc gcaatcatcc gctcgtaaca gcttcgccag
     3601 cgatcccttg cgttcccccg gagcgccgaa gccgttcgcc accgccacca ggtgcaggtc
     3661 gtgattgccc agcacgg
//
LOCUS       DASUOM010000411         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_262070,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000411 DASUOM010000000
VERSION     DASUOM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262070"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFY27_08580"
     CDS             complement(<1..357)
                     /locus_tag="VFY27_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5765609.1"
                     /translation="MHTGRREDGMVDVFAEWVENAPVDRCGIPEGEAAGCSDLFKVRW
                     QESFVAEGGRRLICHFRAPDAESVRMTLRCANVVVEAVWSGSVHRWVPSGKTNSSNGV
                     FLPPLPAGTTSTLGTAR"
     gene            complement(360..1661)
                     /locus_tag="VFY27_08585"
     CDS             complement(360..1661)
                     /locus_tag="VFY27_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5765610.1"
                     /translation="MTRTLKTGGRPRTGTTDVVVIGAGHSGLAVSHFLTALSIDHVIL
                     ERGEIANSWRRERWDSLRLLTPNWLSRLPGYSYTGRQPDDYMSTAEVTRFIADYASFT
                     GAPVQTRTNVTRVALTDNGYRVSTDRGSWSCRAVVVASGACNLAAVPAVSAAVPEGVA
                     QLTALDYKNPQQLEPGGVLVVGASATGLQLADEIRRAGHDVTIAVGEHVRLPRFYRGR
                     DIQWWMQATGVLDQKYTEVDDLVRARNVPSPQLVGSNEQLFMDLNSLSDRGVRVTGRL
                     AGIRNGQAQFSGSLSNVCALADLKMNRLLDTIDKWLEQKGCPDFGTPSERFEPTRVPQ
                     TSCLNLDLNNGAIRTVLWATGYRPDYSWLDVPVLDRKGLIRHEGGVGAVPGLYVTGLP
                     FLRRRKSSFIHGAEDDARELTEHLAGWLASSSPKQLSRIAS"
     gene            complement(1658..2218)
                     /locus_tag="VFY27_08590"
     CDS             complement(1658..2218)
                     /locus_tag="VFY27_08590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5765611.1"
                     /translation="METNQATVNNGVNVEALLGAREALSEAPQAAKFTWRATCDWING
                     THSATTVKSFFGLGEEQQHRTAFRFDTDHPEVFASEDKGATPVEVVLAGLAGCLTAGI
                     ASVAQNRNIQLRSVKATLEAGMDLQGILGIDGDVRNGFDGIKVHYDIDADASKEDIAA
                     LVAQSQKRSAVFDIITNPTDVTVTVA"
     gene            complement(2389..>3228)
                     /locus_tag="VFY27_08595"
     CDS             complement(2389..>3228)
                     /locus_tag="VFY27_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765612.1"
                     /translation="WQHGQWAGAREQSLQSERVARGGSDRDQITAMADTAKCLLMLER
                     DLSQADALLMEAEALAARQHIRHHAIPAGRAMLHYYENRLDDAEELFKQARTLCKYAG
                     DRVSEYQANEYLVMINFQQGRYAEAQARCAELVALGRKLREGSEAPFANALQGLCAYA
                     IDDTSAELDSALGLLRIADAKHRLAYVQTRAALLDYEHQRIEQALERGGEALACAEML
                     ERTTEMMLAHLVLGLCYRAGGRHTEAARHFTSVDALEAAGVAEWARQMVRALSGTGEK
                     LQA"
BASE COUNT          538 a         1040 c         1022 g          628 t
ORIGIN      
        1 gcgcgccgta ccaagggtgg acgtcgtgcc agccggaagc gggggcagga agacgccgtt
       61 ggacgagttc gtcttgccgc tcgggaccca cctgtggacg ctgccgctcc agaccgcttc
      121 taccacgacg ttcgcgcatc gaagggtcat gcgaaccgat tccgcgtcgg gagctctgaa
      181 atggcaaatc agccgccgtc cgccttcagc gacgaagctt tcctgccagc gcactttgaa
      241 aaggtctgaa cagccggcgg cctccccctc gggtatcccg cagcggtcga ccggcgcgtt
      301 ttccacccat tcggcaaaca cgtcgaccat cccgtcttcg cgcctgccgg tgtgcatgat
      361 caggacgcta tcctgctaag ctgtttcgga ctgctgctcg caagccagcc ggccaggtgc
      421 tccgtcagct cgcgcgcatc gtcctccgcg ccgtggatga agctcgactt gcggcggcgc
      481 aggaagggga gtcccgtgac gtagagaccg gggacggcgc cgacaccgcc ttcatggcgt
      541 atgagtccct tgcgatccag caccggaacg tcgagccatg aatagtcggg gcgatagccg
      601 gttgcccaaa ggacggtgcg tatcgcaccg ttgttcaaat cgaggttcag gcacgaggtt
      661 tgaggaacgc gtgtcggttc gaagcgttcg gagggtgtcc cgaaatccgg acatcctttt
      721 tgttcgagcc acttgtcgat cgtgtccagc agccggttca tcttcaggtc ggcgagagcg
      781 cagacgttgc tcaacgaacc cgagaattgg gcttggccgt tgcggatgcc ggccagcctg
      841 cccgtgacgc gcacgccgcg gtcggacagc gagttcaggt ccatgaaaag ctgctcgttc
      901 gagccgacaa gctgcggtga cggcacgttt cgcgcgcgca ccaggtcatc gacctcggtg
      961 tacttctggt cgagcacgcc ggttgcctgc atccaccact gtatgtcccg gccccgataa
     1021 aaccgcggca gccgcacgtg ctctcccacc gcgatcgtga cgtcatgccc ggcacgccgg
     1081 atctcgtctg cgagctgcag cccggtggcc gatgcgccga caaccagcac accgcctggc
     1141 tcgagctgtt gcggattctt gtagtccagc gcagtcagct gggctactcc ttcaggcacc
     1201 gcggcactta ctgccggcac ggccgccagg ttgcaggcgc cgctcgcaac gacgacggcg
     1261 cggcatgacc agctcccacg atcggtgctg acgcgatagc cgttgtccgt gagcgcaaca
     1321 cgagtaacgt ttgtccgtgt ttgcacgggc gcgccggtga agcttgcgta atcggcaatg
     1381 aaccgggtca cttcggccgt ggacatgtag tcgtcgggct gccgtccggt atagctgtaa
     1441 cccggcagcc tggagagcca gttcggcgta agcaggcgca gcgagtccca gcgctcgcgc
     1501 cgccatgaat tggcgatctc cccgcgctcc aggatcacat ggtcgatgga aagagcggtc
     1561 aggaaatggc tgaccgccag cccggagtga ccggcaccaa tgacgacgac gtcagtggtg
     1621 ccggtgcggg gacggccgcc tgttttcagc gtccttgtca cgcgacggtt accgttacat
     1681 ccgtagggtt ggtaatgatg tcgaacaccg ccgaccgctt ctgcgactgg gcgaccaggg
     1741 cggcgatatc ctccttggag gcatcggcat cgatgtcgta atgaaccttg atgccatcga
     1801 agccgttacg cacgtcgccg tcgatgccga gaatgccctg caggtccatt ccggcctcaa
     1861 gcgtcgcctt caccgagcgc agctggatgt tgcggttctg cgcaacggaa gcgatcccgg
     1921 cggtcaggca accggccagg cctgcaagca cgacttccac cggcgtcgca cccttgtctt
     1981 cggaggcgaa aacctcggga tggtcggtat cgaacctgaa cgccgtgcgg tgctgctgct
     2041 cctcgccgag gccgaaaaag ctctttacgg tggtcgcgct gtgcgttccg ttgatccagt
     2101 cacaggtcgc gcgccacgtg aatttcgcgg cctggggcgc ttcggacagc gcttcccgcg
     2161 cgccgagcag tgcttcgacg ttgactccgt tgttgacggt agcctggttg gtttccattc
     2221 ggtttgctcc ttgggtcatt gatcagatgg taagcccggc tcgtgccggg caggagcgaa
     2281 caatgcggcc tgcgcgttag attgagcgtg aaagcagccg tggtatttat gcttcggccc
     2341 ggccggctcc gacaggctta ccccgatacg ctacaacgaa tcggtctttc aggcctgcag
     2401 cttctcgccc gttcccgaga gcgccctgac catttgcctc gcccattcag ccacgcccgc
     2461 ggcttccagg gcatcgacgg aggtgaagtg gcgtgctgcc tccgtgtgcc tgccgccggc
     2521 gcggtagcac aatcccagca ccaggtgtgc aagcatcatt tccgttgttc tttcaagcat
     2581 ttctgcgcag gcgagcgcct ccccgccgcg ctcgagggct tgctcgatgc gctggtgttc
     2641 gtagtcgaga agcgccgcgc gtgtctgcac gtaagcaagg cggtgcttcg catccgcaat
     2701 gcggagaagg ccgagcgcgg agtcgagttc cgctgaagta tcgtctatcg cgtaggcgca
     2761 caggccctgc agggcgttcg caaacggagc ctcgctgcct tcgcgcagct tcctgccgag
     2821 cgctaccaac tccgcacagc gggcctgtgc ctcggcgtag cggccctgct ggaagttgat
     2881 catcaccagg tattcatttg cctggtactc ggagacccgg tcgccggcgt acttgcataa
     2941 ggtcctggcc tgcttgaaga gttcctcggc atcatcgagc ctgttttcgt agtagtgcag
     3001 cattgcgcga ccagcgggaa tggcgtggtg ccggatgtgc tggcgcgcgg caagcgcttc
     3061 agcctccatc agcagggcgt ccgcctgcga aaggtcgcgc tccagcatca gcaggcactt
     3121 ggcagtgtcc gccatagcgg tgatctggtc cctgtcgctg ccgccgcgcg cgacgcgttc
     3181 cgattgcagg ctttgctccc gggcacccgc ccattgaccg tgctgcca
//
LOCUS       DASUOM010000412         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1236707,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000412 DASUOM010000000
VERSION     DASUOM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236707"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3782)
                     /locus_tag="VFY27_08600"
     CDS             complement(<1..3782)
                     /locus_tag="VFY27_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5765613.1"
                     /translation="MSPEDHALPPDNFQEDPKPVVAHRTSPTNIGLYLLSTLVARDLG
                     WLGTLEASERLEATLATMSGLELFRGHLYNWYDTRTLHPLDPKYVSSVDSGNLAGHLV
                     VLSNGCRELIQQSTVDVNLFAGIDDAIRLLREALTEIPDQRQTHTVTRKQLSQAVEAL
                     AMLLLSRPLDAIGWTTRFGRLTALSNTVADMAQALAQERSDGPEGELRIWADSVRKCV
                     ESHLRDVELLLPWVRFSAKDLASMFDSPSGPVLEWETIEPFFHSVPTLAVAPERFAAA
                     LRALDRLREDSLSHPLQNRASLARIALLADAIRQSSADATALIRRLAVIAQTAEQMVR
                     AMDFTFLFDPTRKLLSIGYRVAENSLDPNCYDLLASEARLASFIAIAKGDVPASHWFH
                     LGRALTPLGDGSALISWSGSIFEYLMPALVMRFPAGSLLSRTYELIVRRQIQYGAERR
                     VPWGVSESAYNARDLNLTYQYSSFGVPGLGLKRGLSEDIVIAPYAAALAAMIDPAAAT
                     QDFALLAEAGGKGTYGFYEALDYTGTRVPEGKDVAIVRAYMSHHQGMSLVAIANVLND
                     GVMRNRFHAEPIVRATELLLQERTPRDVPVARPRAEEVSAAAQVRDLIPPMSRRFTSP
                     HEATPRTHLLSNGRYAVMLTVAGSGYSRWRDIAVTRWREDTTRDCWGSYIFLRDAQTG
                     SVWSAGYQPSGVEADDYEVSFSEERAEIIRRDGDWSTTFEVVVSSEDDAEVRRISVTN
                     MGTRARDLQVTSYAELSLAPQAVDVAHPAFSNLFVQTEFVPEISALLATRRRRSDDDA
                     AVWAAQVVVVEGETVGELQYETDRARFLGRGHHVRTAVSMIDGRPFSNSVGSVLDPMM
                     SLRRTVRVLPGGTARIVFSTIVGASRDQVLDLADKYSDPRVFERALSLAWTQTQVQLH
                     HLGIGIDEAQLFQRLANAVLYSDAALRPSSETLESATLERASLWAHGISGDLPIVLAC
                     IDKAEDVDIVRQLLRAHEYWRMKQLSADVIILNEKPASYEQDLQGSLEALVRGSRLRL
                     LPDTGGARGSIYLLRSELLSPQDRTLLQHFARVVLLSRRGTLAEQIARLQRRRLAPPP
                     LLPSRRGSTRQDVPLPEREVEFFNGLGGFADDGREYVTILSEGLRTPRPWINVIANPL
                     FGFLVSESGSGFTWSLNSHENQLTPWSNDSVTDVSGEMLYIRDDDSEELWSPTALPIR
                     DDPAPYVACHGQGYSRFHHGSHGILVELLQFVPPEDPVKISRLTLQNLSGRSRRLTV"
BASE COUNT          818 a         1207 c         1205 g          703 t
ORIGIN      
        1 accgtaaggc gacgcgagcg gccggacaaa ttctgtagcg tgagccgaga aatcttgacc
       61 ggatcttcag gcggcacaaa ttgcagcagt tcgaccagga taccgtgcga accatgatgg
      121 aagcggctgt agccctgtcc atgacaggcg acatagggtg ccgggtcatc gcgaataggc
      181 agtgccgtgg gactccagag ttcctctgag tcgtcatcgc ggatatagag catctctccg
      241 gacacatcgg tgaccgaatc gttcgaccag ggcgtcagtt gattctcgtg gctgttcagc
      301 gaccaggtga atccggaacc ggattccgac acgagaaacc cgaacaatgg attcgcaatg
      361 acgttaatcc agggccgcgg cgtccgcagc ccttcgctaa gaatcgtgac gtattcccgg
      421 ccgtcatctg cgaagccgcc aaggccgttg aagaattcga cttcccgttc aggcaacggc
      481 acgtcctgac gtgtgctccc gcgccgtgat ggcaggagcg gcggcggggc aaggcgtctg
      541 cgctgtaatc gagcgatctg ctctgccaac gtgccccgcc gactcaacag gacgacccgg
      601 gcgaaatgct gcaggagcgt tcgatcttgc ggagagagca gttcgcttcg cagaagataa
      661 atgctgccgc gtgcgccgcc ggtgtcgggc agaaggcgca accggctgcc gcgcacgagc
      721 gcctccaggg agccttgcag atcctgctcg taggacgccg gcttttcatt caaaatgatc
      781 acgtcggctg ataattgctt cattcgccag tattcatgtg cgcgcagcaa ttgccggacg
      841 atatcgacat cctctgcctt gtcgatgcag gccagcacga tggggagatc gccggagatg
      901 ccatgtgccc acagcgacgc acgctccagc gtggcggact cgagtgtctc ggaagaggga
      961 cgcaacgcgg catcggaata cagcaccgca ttcgccagcc gctgaaacag ctgcgcttcg
     1021 tcgatcccga taccaaggtg atgcagttgc acttgcgttt gcgtccaggc gagcgacagg
     1081 gcacgctcaa acactctcgg gtcactgtat ttgtcagcga gatccaacac ctggtcgcgc
     1141 gaggcgccga caatagttga aaacacgatg cgtgcggtcc cgccggggag cactcggacc
     1201 gtgcggcgca agctcatcat cggatcgagg acggacccga cggaattcga gaacggccgc
     1261 ccatcgatca tcgagacagc cgtacggaca tggtgcccgc gcccgagaaa cctggcgcga
     1321 tccgtttcat attgcagttc acccacggtt tcgccttcaa cgaccacgac ctgggcagcc
     1381 cacaccgctg cgtcgtcatc cgatcgccga cggcgtgtcg cgagcagcgc gctgatctcg
     1441 ggtacaaatt cggtttgcac aaacaggttc gaaaaggctg gatgggccac atcgacagct
     1501 tgtggagcca gggacagctc ggcatacgag gtgacctgta gatcgcgcgc tcgtgtcccc
     1561 atgttcgtca cggagatgcg ccgcacttcc gcatcgtcct cggacgacac cacgacctcg
     1621 aaggtcgtgc tccagtcgcc atcccgtcgg atgatttcgg cccgttcctc ggaaaaggac
     1681 acttcatagt cgtcggcctc cacgccgctt ggctgatacc cggccgacca cacagacccg
     1741 gtctgcgcgt cgcgcaggaa aatgtaggag ccccagcagt cccgcgtcgt atcctcccgc
     1801 cagcgcgtca ccgcaatgtc gcgccaccgg ctgtaccctg aacctgcgac ggtcagcatg
     1861 acggcgtacc ggccgttgga gagcagatgg gttcgcgggg tcgcctcatg cggagaggtg
     1921 aatcgccgcg acatcggagg aatgaggtcg cggacttgcg ccgcggcaga gacttcttcg
     1981 gcgcgtggtc gcgcgaccgg cacgtcgcgg ggtgtacgtt cctggagcag gagctcggta
     2041 gccctgacaa tcggctcggc gtggaaacgg ttcctcatca ctccatcgtt caggacattc
     2101 gcgattgcga ccaacgacat cccctgatgg tgcgacatat aggcgcgcac gatggcaacg
     2161 tcttttcctt cgggcacccg cgtgccggta taatccaatg cctcatagaa cccataggtg
     2221 cccttgccac cggcctcggc aagtagggca aaatcttgcg tcgccgcggc cggatcgatc
     2281 atggccgcga gtgcagcggc gtatggcgca atgacgatat cttcactgag gccgcgcttg
     2341 agtcctagcc ccggcacccc aaagctggaa tactgatacg tcaggttgag atcgcgcgca
     2401 ttataggcgg actcggagac accccagggc acgcggcgct ctgcaccata ttgaatctgt
     2461 cgacggacaa tcagctcata cgtccggctc agcaaactgc ctgccggaaa acgcatcacc
     2521 aacgccggca tgagatattc gaagatggag cctgaccagg aaatcagcgc ggaaccgtct
     2581 ccaagtggcg tcagggcacg gcccaggtga aaccagtgcg atgccggcac gtcgcctttg
     2641 gcgatggcga taaagctggc cagccgggcc tccgacgcaa gcaagtcgta gcagtttgga
     2701 tccaagctgt tttcggctac gcgatagccg atggacaaca gcttgcgtgt cggatcgaag
     2761 aggaatgtga aatccatggc gcgcaccatc tgctccgcag tctgcgcgat gaccgcgagt
     2821 cggcggatga gagcagtggc atcggcggac gattgccgta tggcatcagc cagcagagcg
     2881 atccttgcca ggctggccct gttctgcaac ggatggctga gcgagtcttc tcgcaaacgg
     2941 tcgagcgcac ggagcgcagc cgcgaaacgt tcaggggcga ccgccagcgt cggaacggaa
     3001 tgaaagaacg gctcgatggt ctcccactcc agtaccggcc cggacggact gtcgaacatg
     3061 gacgcaagat ctttcgcact gaagcggacc cacggaagca acagttccac gtcccggaga
     3121 tgactctcta cacacttcct gaccgaatcc gcccagatgc gcaactcgcc ctcgggcccg
     3181 tccgagcgct cctgtgccag ggcctgcgcc atatctgcga cggtattgga caatgcggtc
     3241 agtcggccaa accgcgtggt ccagccgatg gcatcgagcg gccgcgacag cagcaacatg
     3301 gctagcgctt caacggcttg gctcagctgt ttccgtgtga cggtgtgcgt ctgtcgctgg
     3361 tcgggaatct cggtgagcgc ctcacgtaat aaccggatgg catcgtctat tccagcaaat
     3421 agattgacgt cgacagtaga ttgttgaatc aactcacggc atccgttgct caagaccacc
     3481 agatgtcccg cgagattgcc gctatccacg gaggacacat acttcggatc gagcggatgg
     3541 agggtgcgtg tgtcgtacca gttgtagagg tgcccgcgaa atagctcgag gccgctcatg
     3601 gtcgccagcg tggcctccag tcgttcgctc gcttccagag tgcccaacca accaaggtcg
     3661 cgggcgacca gtgtcgagag caaatagagc cctatattcg ttggagacgt ccggtgggcc
     3721 acgacgggct tcggatcttc ctgaaagtta tccggtggca gcgcatggtc ttccggtgac
     3781 acaaatgtct cgaagaacct ccaggtccgg cgcgcaatca accggagcgt ccgggcatcg
     3841 gcgggcgtaa ccggctcagc gccggtgaac cgcggaggca ggctgaccca gcgcgccacc
     3901 gccggagccg ccatccatag gaggataaac ggg
//
LOCUS       DASUOM010000413         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1711934,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000413 DASUOM010000000
VERSION     DASUOM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711934"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VFY27_08605"
     CDS             <1..1665
                     /locus_tag="VFY27_08605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5765614.1"
                     /translation="DVVRLELGHAAKARRFPKKDTCLAIYSRCVNAGTPLAAALAGTW
                     PWCAEWEQELRTIFRRYVEVKQESHALDYDDLLLYWSHLVADPELAAEIGSWFDHLLV
                     DEYQDTNLLQAGILRALKPHGSGLTVVGDDAQSIYSFRAADIENILGFANGFMPPAEV
                     VSLEENYRSTQPILDSANCLIAQSDRLYRKTLVSHSTGGGAKPQLVTVEDLEAEAEYV
                     VEAVLANRECGTELRKQAVLFRSAHHSDRLELELVRRSIPYVKFGGLKFLEAAHVKDL
                     LSILKWAENPRNKMAAFRVLKLLPGMGPALTSRCIDCLALGNHSFSALAGFQAPAAAR
                     ADWPAFSSLMQALSAAEAEDGAWQAQLAGARRWYQPHLERLYDSVDTREADLEQLEQI
                     SGRYPTRERFLSDLTLDPPAAAGDLAGHPLLDEDYLILSTVHSAKGQEWDSVFLLNFS
                     DGNFPSEFATGKPEMIEEERRLLYVAMTRAKQSLHLVAPLRYHVTRQRRDGDRHVYGA
                     RSRFMTDALMRTMDAIFRGRADGPEGHMSPRSDRVLDVAARLKEMW"
     gene            complement(1674..2090)
                     /locus_tag="VFY27_08610"
     CDS             complement(1674..2090)
                     /locus_tag="VFY27_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX5765615.1"
                     /translation="MDLGNAIAGLNWLAVFLAAGTAFFIGAVWYSRKVFGNAWMMEVG
                     LTEETISRGNLALTFGGTFALQVVAATALAFFLGSGSDWLIGLQAGLLVAVCWIGTAY
                     GITYFFEQRSLRLYLINAGYYLVLFAAMGAIIGAFN"
BASE COUNT          490 a          728 c          700 g          444 t
ORIGIN      
        1 gacgtcgttc ggctcgaact cgggcatgca gcaaaggcaa ggaggtttcc gaagaaggat
       61 acctgccttg caatctattc ccgatgcgtt aacgccggta caccgctcgc cgctgcgctc
      121 gccgggacgt ggccgtggtg cgctgaatgg gagcaggaac tgcggacgat tttccgccgc
      181 tacgtcgaag taaaacagga gagccacgcg ctggactacg acgacctgtt gctttactgg
      241 agccatctcg tcgcagatcc agaactcgcc gccgagatcg gctcgtggtt cgatcacctg
      301 ctcgtcgacg aataccagga tacgaacctg ctgcaggcgg gcatcctgcg ggcgttgaag
      361 cctcacggta gtggacttac cgtggtcggc gacgacgcgc agtccattta ttcctttcgc
      421 gccgccgaca tcgagaacat tcttggattc gcgaacggct tcatgcctcc ggccgaagtc
      481 gtcagcctgg aggaaaacta tcgctccacg cagccgattc tcgacagcgc caactgcctc
      541 atcgcgcaga gcgaccggct gtaccggaag acgctcgtat cgcattcgac aggagggggt
      601 gcgaagccgc aactcgtgac ggtagaagat ctcgaggctg aagccgagta cgtcgtcgag
      661 gcggtgctgg cgaaccgcga atgcggcacc gaactccgta agcaggcggt actgttccgc
      721 agcgcgcacc acagcgacag gctcgaactc gaactcgtcc ggcgcagcat tccgtacgtc
      781 aagttcggtg gcctcaagtt tctcgaggcg gcgcacgtca aggacctgct gtcgatcctc
      841 aagtgggccg agaacccgcg caacaagatg gcggcgttcc gcgtgttgaa gctcctgccc
      901 ggcatggggc cggctcttac ttcgcgctgc atcgactgcc tcgcactcgg caatcacagc
      961 ttttccgccc tcgcaggatt tcaagcgcct gccgctgcgc gcgcggactg gcctgccttc
     1021 tcgagcctga tgcaggcgct ctcggccgca gaggcggagg acggtgcctg gcaagcgcag
     1081 ctcgctggcg cccggcggtg gtatcagccg cacctcgaac ggctctacga cagcgtcgac
     1141 acgcgcgagg ccgatctgga gcaactcgag cagatttccg gccgctatcc gactcgcgaa
     1201 cgttttctct ctgacctcac gctggacccg ccggcagctg ccggggatct cgcgggccat
     1261 ccgttactcg atgaggacta cctcatcctg tcaacggtgc actcagcgaa aggccaggaa
     1321 tgggactcgg tgttcctcct gaacttttcc gacggcaatt tcccgtcgga gttcgcgaca
     1381 ggcaagcctg agatgatcga ggaagaacgc cgactgcttt acgtggcgat gacgcgcgcg
     1441 aagcagtcgc ttcatctcgt tgcaccactc cgctatcacg ttacccggca acgacgtgac
     1501 ggcgaccggc acgtctacgg tgcgcgcagc cggttcatga ccgatgcgct gatgcgaacc
     1561 atggacgcga tatttcgcgg gcgagcagac gggcccgagg gtcacatgtc accgcggagc
     1621 gatcgcgtcc tcgacgtagc cgcccgcctg aaagagatgt ggtagcaggg actctaattg
     1681 aacgcgccga taatcgcacc catcgctgcg aacagcacca ggtaatatcc tgcattgatc
     1741 aggtatagcc ggagcgaacg ctgctcgaag aagtaagtaa taccgtatgc agtgcctatc
     1801 cagcagactg ccaccagcaa gccggcttgc aggccgatga gccagtcact gccggacccg
     1861 aggaagaacg cgagcgccgt cgcagcaacc acctgtagcg caaatgtgcc gccgaaggtg
     1921 agagcgaggt tcccgcggct gatagtttcc tcggtgaggc caacttccat catccatgca
     1981 ttgccgaaaa ctttcctgga ataccagacg gcgccgataa agaatgcggt gccggccgca
     2041 aggaaaaccg ccagccagtt caagcccgcg atggcattgc ccagatccat gactatctcc
     2101 tgttgttgtt gttcgttgtg ccaggtttca cggagcgcaa gcggccagtc taccaaacct
     2161 gcagtacaca gcgcgagcag caggtcttcg acaccaagta gtaaagagcg atcgatgttg
     2221 cagaatgcgc atcagccgca tcctgccgta accaatcgtg aaccttgtca caatgcctac
     2281 gctctaattc ctatatatgt agttgcgcct ggttcgattt tttggcggaa ttgagcaaag
     2341 gcgctgtagt gcggtgtact ca
//
LOCUS       DASUOM010000414         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1226194,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000414 DASUOM010000000
VERSION     DASUOM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226194"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..717)
                     /locus_tag="VFY27_08615"
     CDS             complement(97..717)
                     /locus_tag="VFY27_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019563116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5765616.1"
                     /translation="MRLKHALLALSILFAATARSEPLVEGSAEAGQARSVTCAACHGP
                     DGNSVNPAWPSIAGQNAPYILKQLQAFKSGSRTDPLMTAQAMPLSEQEMRDLAVYYAG
                     QTAAPKTVAAPESVNKGEALYQGGDAENSVAACLACHGPTGRGNPAAAYPMLKGQHAA
                     YTATQLRNYATGQRKSDGPTRVMRDIAARLEEDDILAVASYVQGLQ"
     gene            967..1581
                     /gene="yihA"
                     /locus_tag="VFY27_08620"
     CDS             967..1581
                     /gene="yihA"
                     /locus_tag="VFY27_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5765617.1"
                     /translation="MSQYPDAQFLLSADAPAQFPPDTGVEVAVAGRSNAGKSSAINVI
                     VNRRQFARTSKTPGRTRLVNFFGLREGQRLVDLPGYGFARVSDEQRDHWGDLLQHYFE
                     ERKSLSGLILVVDVRRQLMDFDRQMLAFSSFVDLPVHILLTKADKLRRGQASRALLEV
                     RQAAGADVSVQLFSATTRQGLDEARQVLARFLDRPNPPDDARQS"
     gene            complement(1846..>2885)
                     /locus_tag="VFY27_08625"
     CDS             complement(1846..>2885)
                     /locus_tag="VFY27_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX5765618.1"
                     /translation="RQLQQILFEEQGLPVIRRTPTKQPSTGEDVLQELALRYPLPRVI
                     LDYRSVSKLKSTYTDKLPLQISSRTGRVHTCYQQAVAATGRLSSTDPNLQNIPIRTAE
                     GRRIRQAFIAPPGQLLMAADYSQIELRIMAHLSGDPGLVSAFAEDEDIHRATAAEVFG
                     VPLDEVTPDNRRAAKVINFGLMYGMSAFGLARQLGVARGEAQEYVDLYFERYPGVRQY
                     MDNTREQARRRGYVETLFGRRLYLPEINDRNAQRRQYAERSAINAPMQGTAADIIKKA
                     MVAVHAWLADEAVPARMIMQVHDELVLEVADDAIDRVRESVVSLMSAAADLSVPLKVD
                     AGVGVNWDEAH"
BASE COUNT          466 a          905 c          949 g          565 t
ORIGIN      
        1 ttatcgaatc gtttcagtac gcgagccggg ggtattcgat gcttgtccct ggcgcttagt
       61 tcctggtctt gcctgcaggc cgccttcttc tgcccgctat tgcaagccct ggacatagga
      121 tgcgacggcg agaatgtcgt cttcctcgag gcgtgccgcg atgtcccgca ttacccgggt
      181 cgggccatcg gacttgcgct ggccggttgc gtagttcctg agctgggtgg cggtataggc
      241 tgcgtgctgg cctttgagca tgggatacgc ggccgcggga ttcccgcgcc cggtggggcc
      301 gtggcaggcg aggcatgccg cgacactgtt ttccgcgtcc cccccctggt agagcgcctc
      361 tcccttgttg accgattccg gagcggcaac tgtcttcggc gcggcagttt gtccggcata
      421 gtagacggca agatcacgca tctcctgctc ggacaacggc attgcctgtg cggtcatcag
      481 gggatcggtg cggctgcccg acttgaacgc ctgcaactgc ttcagtatgt aaggggcgtt
      541 ctgcccggcg atgctgggcc acgcgggatt cacgctgttg ccgtccgggc cgtgacaggc
      601 ggcacaggtc acggatctcg cttgtcccgc ttcggcggaa ccctctacga gcggctcgga
      661 gcgggcggtc gcagcaaaca atatcgacaa ggcgaggagc gcgtgtttca gtctcataag
      721 catccagtca gcaatgcagg ccgcagtcgt aaagcagctg gcggcgctgc cgtcatagca
      781 gtggcaggat cggcacaaaa gagtccccgg agctggcggt tgttgtccgc gtcaccgcgg
      841 cctttgcttg cggctccagc ctcgggcagt atatcggccg cctttggtta gtcgaaggcc
      901 atgtccggca acgctcaagc cattaatgcc aggctccaaa ttatacacag atcggggccg
      961 tatcccatgt cacagtaccc ggacgcgcaa ttcctgctga gtgccgatgc gccggcgcag
     1021 ttcccgcccg ataccggcgt cgaagttgcc gtggccggcc gttcgaacgc cggcaagtcc
     1081 agtgcgatca acgtcatcgt caatcgcagg cagtttgccc gtaccagcaa gacgcccggc
     1141 cgcacgcggc tcgtcaactt cttcggtttg cgggaaggcc agcggctggt cgaccttccc
     1201 ggctacggct ttgcgcgggt ttccgacgag cagcgtgacc actggggcga cctgttacag
     1261 cactatttcg aagagcggaa gtccctgtcc gggcttattc tcgtggtaga cgtgcggcgg
     1321 caactgatgg atttcgaccg ccagatgctc gccttctcct cgttcgtcga cttgccggta
     1381 cacatactcc tgaccaaggc ggacaagctc aggcggggac aggcgagccg ggcactcctc
     1441 gaggtgcggc aggcagccgg ggcggacgtt tctgtgcagt tattctcggc gacgacgcgc
     1501 cagggcctgg acgaggcgcg gcaggtgctg gcgcgctttc tcgatcggcc gaacccgccg
     1561 gacgacgcac gtcagagctg acaccaggag aaacgaaaaa aaaaccaccg ggcgccgcag
     1621 acgggcgccc ggggtcagta acgcaaccgg cttggggtga ccgtgttgca acacctgcct
     1681 agggaggtaa gcaggcgagt gccttggcac tagcggtttt gaccggccgg gggcggagag
     1741 ttcccggccg cacgattttt tggcttcgtc acttgcgatc agctccccgc ccgcgggcac
     1801 gtatcgttca cgaagcgctc tggtgtgggc ctctacagca ctctgctagt gcgcctcgtc
     1861 ccagttcacc cctacgccgg cgtccacttt cagcggcacg gacaggtccg ccgccgcgct
     1921 catcagcgag acgacactct cgcgcacgcg gtcgatggcg tcgtcggcga cttcgagtac
     1981 cagttcgtca tgcacctgca tgatcatgcg ggccggtacg gcttcgtcgg cgagccacgc
     2041 gtgaaccgca accatggcct tcttgatgat gtcggccgca gtaccctgca tgggcgcatt
     2101 gatcgcgctg cgctccgcgt actgcctgcg ctgcgcattg cggtcgttga tctccgggag
     2161 gtagagccgc cgtccgaaca gggtttccac gtaacccctc cggcgcgcct gctcccgggt
     2221 attgtccatg tactgcctga cgccggggta gcgctcgaaa tacaggtcca cgtactcctg
     2281 cgcttcgccg cgagccacgc cgagttgccg ggccagtccg aaagcggaca tgccgtacat
     2341 aaggccgaag ttgatcacct tggcagcgcg gcggttgtcc ggcgtaacct cgtcgagcgg
     2401 cacgccgaag acttccgcag ccgtcgcccg gtggatgtcc tcgtcttcgg cgaatgccga
     2461 cacgagtcct gggtcgccgg aaaggtgcgc catgatgcgc agctcgatct gtgaataatc
     2521 cgccgccatg agcagctggc cgggcggggc aatgaaggcc tgacggatgc gccggccttc
     2581 ggcggtacgg atcgggatgt tctgcaggtt cggatccgtc gaggacagcc gcccggtggc
     2641 ggcgaccgcc tgctggtagc aggtatgcac gcgtcccgtg cgcgagctga tctgcagcgg
     2701 cagcttgtcg gtgtaggtgc ttttgagctt gctgacactg cggtagtcga ggatgacccg
     2761 cggcagcggg tagcgcaggg cgagctcctg caggacgtcc tcgccggtcg atggctgttt
     2821 tgtgggtgtc ctgcggataa ccgggaggcc ctgttcctcg aacaggatct gttgaagctg
     2881 cctcg
//
LOCUS       DASUOM010000415         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1398294,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000415 DASUOM010000000
VERSION     DASUOM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398294"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFY27_08630"
     CDS             complement(<1..396)
                     /locus_tag="VFY27_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765619.1"
                     /translation="MEQERAEQKTGNERWPLIRDVLVFQCKLVVDGLRDLILLPVSLV
                     AGLVSIIGKGKGSPPGREFYDLLHAARRSERWINLYGAAEKRGQGHKPTAQDPPATTP
                     DLDALVARVETFLVEEYRKGDITAQTKARM"
     gene            618..983
                     /locus_tag="VFY27_08635"
     CDS             618..983
                     /locus_tag="VFY27_08635"
                     /inference="COORDINATES: protein motif:HMM:NF023903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HEX5765620.1"
                     /translation="MNPVRRTAFNTEIALAKERISEGDLDTGLVYLERAHIIGQAFVK
                     PHASSHWLMLKLELRRRRPAAAFGQAVRIVLGVLGSAVGVVPVGNTGSSNVSMFKRMP
                     IPPELQKIIDGEAPPDAPA"
     gene            complement(1032..1892)
                     /locus_tag="VFY27_08640"
     CDS             complement(1032..1892)
                     /locus_tag="VFY27_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX5765621.1"
                     /translation="MSYVRFFLMILTSTVVMFILMYLNTYAWEHVFFSETRTYMAIVM
                     GAAMAVIMLTYMLGMYSNKPLNIAIFAGAVIVFALSLWLVRSQVTVSGPSYMRAMIPH
                     HSIAVMTSERAQIRDPRVRKLADEIIDAQRREIAEMRYLIADVSSGNVIESIYHDPPA
                     RPGTVEDALANTLISQLDLAPMPKAEADRVLSADVRCTFNRSPEADPILWAARDGTAA
                     AVKLNGILVQLDVDDALQGGTARFSARGSRISVQPLGESADWRGNADLVFELDRGLTV
                     GYRGFYDCET"
     gene            complement(2022..>2490)
                     /locus_tag="VFY27_08645"
     CDS             complement(2022..>2490)
                     /locus_tag="VFY27_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX5765622.1"
                     /translation="EFQGLYSRAIAPFFDIQTGVRQDFGPEEDRTHVVLGLQGLVPYA
                     FELDVAAFLSHEGELTGRVELEYDLQIGQRLLLQPRIEASLAAQEMPELGVGSGLGSV
                     ETGIRLRYEIRREFAPYVGIAWQHKYGGTADLARAAGEDSSTWQLLAGVRSWF"
BASE COUNT          512 a          767 c          736 g          475 t
ORIGIN      
        1 catgcgtgct ttcgtttgcg cggtaatgtc ccccttgcgg tactcctcga cgaggaatgt
       61 ctcgacgcgc gcaaccaggg catcaaggtc aggcgtcgtg gcgggcggat cctgggcggt
      121 aggcttgtgg ccttgccctc gcttttcggc agctccgtag aggttgatcc agcgctcgct
      181 ccggcgcgcc gcgtgcaaca gatcgtaaaa ctccctcccc ggcggcgatc ccttgccctt
      241 gccaatgatg ctgaccaggc cggcgaccag cgacaccggc aggaggatga gatcgcgaag
      301 gccatcgacc accagcttgc actggaacac gagcacgtcc cgtatgagcg gccagcgctc
      361 gttgcctgtc ttctgttcgg ctcgttcttg ttccatcgga gactccgcga ctggtgacag
      421 ctggctcgag catcttaata cgcggcgact gacctcgcgc tgccgcggag ggatttgttg
      481 aatgagaagc attcataact cgcaccataa cgcccaggcg cgtataaggt gatcacgcaa
      541 acgtcccgca gcgcactcgc gccggtcaga tcgcgcaaca tggctacata tccatggtca
      601 gtcacatcgg agggttcatg aatcctgttc gccgcacggc tttcaatacc gagatagcgc
      661 tggcgaagga acgcatttca gaaggcgatc tggacacagg ccttgtgtac ctggagcgcg
      721 ctcatatcat cggccaggca ttcgtaaagc cccatgccag ctcgcactgg ctgatgctca
      781 aactggagct tcggcgacgg cgacccgcag ccgccttcgg acaggccgtg cgcatcgtcc
      841 tgggtgtctt gggttccgcg gtcggtgtcg tccctgtcgg gaacaccggc agcagtaacg
      901 tcagtatgtt caaacgaatg cccatcccgc ctgagttaca aaagattatc gatggcgagg
      961 caccgcccga cgcgcctgcg tagcctgtct ggcaacgatt agccatgcat gcaaacagcg
     1021 gtaacgctca ctcaggtttc gcaatcgtaa aagccacggt aacccaccgt caggcctcgg
     1081 tcgagttcga agacgagatc ggcattcccc cgccaatctg ccgactcgcc gagcggttgg
     1141 accgagatcc tgctgccgcg cgccgaaaac cgggcggtac cgccttgtag cgcatcatcg
     1201 acgtcgagtt gcaccaaaat cccgttgagc ttgacggccg cggcagtgcc gtccctggca
     1261 gcccaaagaa tgggatccgc ttcgggactc cgattgaaag tgcagcggac gtccgccgaa
     1321 agcacacgat cagcttctgc tttcggcatc ggcgcaaggt cgagctggct gatcagcgtg
     1381 ttcgcgagag catcttccac ggtgccggga cgggccggtg ggtcatgata aatactctcg
     1441 atgacatttc cggacgagac gtcggcgatc agatagcgca tttcggcgat ttcgcgccgc
     1501 tgcgcgtcga taatttcgtc ggcgagcttg cggacgcgcg ggtcgcggat ttgtgcacgc
     1561 tcggaggtca tgacggcgat agagtgatgc gggatcatcg cccgcatata gcttggacca
     1621 gagactgtga cctggctgcg aacgagccac agggaaagcg cgaagacaat tactgcgcct
     1681 gcaaatatgg caatgttcag cggcttgttc gaatacattc ccaacatata tgtcagcatg
     1741 atgaccgcca tggccgcacc catcacgatc gccatgtaag tgcgcgtctc cgaaaagaac
     1801 acgtgctccc aggcataggt gttgaggtac atcaggatga acatgaccac cgtagaggtg
     1861 aggatcatca agaaaaaccg cacgtaggac attcggctgt ctcctgttgt tatcctgtct
     1921 gcggaccgga caccgctagt cttcggcggt aaaggcccgg aagcgcggcg acaaaagcag
     1981 tcagggccat gaacgccccc gagtggtaaa ggatcagttt tttaaaacca gctccttact
     2041 cccgcgagaa gctgccaggt gctgctgtct tcaccggcgg cacgggccag gtcggccgta
     2101 ccgccatact tgtgttgcca ggcgatcccg acgtaaggcg cgaactcccg ccggatttcg
     2161 tagcgcaggc gaatgcctgt ctcgacagaa ccgagaccgg aaccgacccc gagttccggc
     2221 atttcctgcg ccgcaaggct tgcctcgatc cgcggctgca ggagcagccg ctggccgatc
     2281 tgcaggtcat actcaagttc cacacgaccg gtcagctcgc cctcgtgcga gagaaacgcc
     2341 gccacatcca gttcaaaggc gtacggcacc aggccctgca gccccagtac cacgtgagtc
     2401 cgatcctcct ccgggccaaa gtcctggcgg acgccggtct ggatatcgaa gaacggcgct
     2461 atggcgcggc tgtagagtcc ctggaactcg
//
LOCUS       DASUOM010000416         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_254457,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000416 DASUOM010000000
VERSION     DASUOM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254457"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFY27_08650"
     CDS             <1..263
                     /locus_tag="VFY27_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765623.1"
                     /translation="FEQVDIYLLPPGRDIADTPPNLPRVATGNSSNYVHLEPGSYAIT
                     VTETGEKSVLAGPLALALSSGDIVEAAILDTADPNVLTLSTY"
     gene            complement(391..2310)
                     /locus_tag="VFY27_08655"
     CDS             complement(391..2310)
                     /locus_tag="VFY27_08655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5765624.1"
                     /translation="MIGPLDLIMTAAMGSSALLYMWLAVRVAGSSSPTESKSVSYFLF
                     LLGTLIAGTAFAYATSNPALYSAGFVLRFFSGGFLPVVLYLIYREYTVGRPPPLLLGV
                     LCIVPLATTLLAITNPFHQMIWSTVSSEPSGRFLASSHVWFTKVHAPFAYALTGYSVL
                     ALAARLPTIAMAHRRNVTLLLVCGALPSAVGVGARLLGIGAVDFPATSLTLTLAFPFY
                     YWVALRARAFQFSPLAYQTLFDRVRDPIIVLDNAQRIISANQPAQLLLAKSELELVGR
                     ELWKDLLAAQIIVDPEKGLDATQTLRMPSDRYYEVTSTALTGPSGQKQGAVVVCRDVT
                     ERMQALRALADSEHLIRSLIEHSSNGMLRFARDQRDPEQKFRCVFANRAAEGYLGSVS
                     GTLVGMPLEKLDTLEPGRLARQFGAGATADPGVSYETAVETADGEVWLRIVAEPVGGD
                     FSVTLIDITQRKRNEAKILADALRDPLTGVLNRRGFEQEAIAGIAACSQGAVLYLDLN
                     QFKSINDRFGHQAGDALLKAFGHRLEFCLRPEDILARLGGDEFAIVLPGVSIEDAKHV
                     AERLVETASEAYIIQGQEIECAASVGIALMPAHGNELWQLVSIADQAMYNAKSISQDE
                     AANDRAAYVEAAVAS"
BASE COUNT          478 a          834 c          835 g          462 t
ORIGIN      
        1 gtttcgagca ggtcgatatc tatctgctgc cgcccggaag agacatcgcc gacaccccac
       61 cgaacctgcc acgggtggca accggcaaca gcagcaacta cgtccacctg gagccgggca
      121 gttacgcaat caccgtgacg gaaaccggcg agaagagcgt tcttgcggga ccgctggcgc
      181 tggcgctctc gtcaggcgac atcgtggaag ccgcgatact ggataccgcg gacccgaacg
      241 tactgacgct ttcgacctac tgaccttaag gagcccgttc cggaagggac cggcagcgtt
      301 ctcgttgtag ttttgccacg gacgggttcg gttaagacaa cctgcgaacg cgcccgcagg
      361 atcaccggtt tggcggcaac tcaatttacg tcaggaagcg actgctgcct cgacgtaggc
      421 ggcgcggtcg ttggcggctt cgtcctggct gatcgatttc gcgttgtaca tggcctggtc
      481 ggcaatgctg acgagctgcc aaagctcgtt gccgtgcgcc ggcatcagtg cgataccgac
      541 actcgccgcg cattcgatct cctggccctg gatgatgtac gcctcggagg cggtctccac
      601 cagccgttcc gcaacgtgct tcgcgtcctc gatcgagact cccggcaata cgatggcaaa
      661 ttcgtcgccg cccaggcgtg ccaggatgtc ctcggggcgc aggcagaact cgagccggtg
      721 accgaaggct ttgagcagtg catcgcccgc ctggtggccg aagcggtcgt tgatggactt
      781 gaactggttg aggtcgaggt agagcacggc tccctgtgag catgcggcaa taccggcaat
      841 cgcttcctgc tcgaagccgc ggcggttaag gacgccggtc agcgggtcgc gcaaagcatc
      901 tgcgagtatc ttggcctcgt tgcgtttgcg ctgagtgata tcgatcagtg tgacggagaa
      961 gtcgccgccg accggctcgg cgactatccg cagccagact tcgccgtccg ctgtttcgac
     1021 ggcggtttcg tagctcacgc cgggatctgc cgtcgcaccc gcgccgaact gccgtgccag
     1081 cctgccaggc tccaaagtat cgagcttttc gagcggcatc cccaccagcg taccgctgac
     1141 gctgcccagg tacccctcgg ccgcgcggtt tgcgaagacg cagcggaatt tctgctccgg
     1201 gtcgcgctgg tcgcgcgcga aacgcagcat cccgtttgac gagtgctcga tgagcgagcg
     1261 aatgaggtgc tcgctgtccg cgagcgcccg cagcgcctgc atccgctccg tcacgtcgcg
     1321 gcatacgacc acagcgccct gcttctgacc ggatggcccg gtcagggcgg tgctcgtcac
     1381 ttcgtaataa cgatcggatg gcatgcggag agtttgcgtg gcatccagtc ctttctcggg
     1441 gtcgacgatg atctgcgccg ccagcaggtc cttccacagt tcgcgcccca ccagctcaag
     1501 ctcgctcttt gccagcaaca gctgcgccgg ttgatttgcg ctgatgatgc gctgcgcatt
     1561 atcgagaacg atgatagggt cccgcacgcg gtcgaagagc gtctggtaag cgagcgggct
     1621 gaactggaat gcccgggccc tgagggctac ccagtagtag aaggggaaag ccagcgtcag
     1681 cgtcagcgag gttgccggaa aatccacggc gccgatgccg agcagcctcg cgcccacgcc
     1741 gacagcggaa ggcagcgccc cgcacaccag caacagcgtc acgttgcgcc ggtgcgccat
     1801 cgcgatcgtc ggcagccttg ccgcgagggc cagtaccgaa tagccggtga gtgcgtaggc
     1861 gaatggcgca tgcactttcg taaaccagac atggctggac gcaaggaacc ggccggatgg
     1921 ctcgctgctg acggtgctcc agatcatctg gtgaaacggg ttcgtgatcg cgagcagcgt
     1981 ggtcgccagg ggcacaatgc agagcacgcc aagcaacagc ggcggcggcc gaccaaccgt
     2041 gtattcgcgg tagatcagat acaggacgac cggcaggaaa ccgccactga aaaacctcag
     2101 gacgaaccct gcgctgtaca gggcgggatt gctggtcgcg taggcgaacg cggtaccggc
     2161 aatcagcgtg ccgagcagga acaggaaata gctgacggac ttcgattccg tcggcgaact
     2221 gctacctgcg acgcggaccg cgagccacat gtaaagcagc gcgctcgagc ccatcgccgc
     2281 ggtcataatg aggtccagcg gtccgatcat cgaaactctc tggacacctt ggccgggccc
     2341 cgcgggtccc ttacgcggcc aggtgccttt cagttgtcat ccgggaaatg ctaacggcgc
     2401 cgcgcccggc atgctgcgac ctgtgccgca ttccgccgct tgatcacagg ggggctcact
     2461 ggcacctgca ggaactcgcc atgcgggcga gtccggttag gccgccgcac tcgcccgcgt
     2521 ggcgggctcc tgcagcggtc tgttcaggtc gcgggagctt cgaggtcttt cgccgtgtct
     2581 gctaccagcc tgtccaccac cgccttcag
//
LOCUS       DASUOM010000417         9334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1621563,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000417 DASUOM010000000
VERSION     DASUOM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621563"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="cysS"
                     /locus_tag="VFY27_08660"
     CDS             complement(<1..1200)
                     /gene="cysS"
                     /locus_tag="VFY27_08660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5765625.1"
                     /translation="MAIHLHDTLTGAKIPFEPLVPGRVTMYLCGPTVYNYAHIGNARP
                     AVVFDVLARLLRRNFTLIFARNLTDVDDKIIAAAKETGEPIGAITERYAEAYRSDMAA
                     LGVKPPDIEPRATEHIAEMIDMIATLLDSGCAYAAEGHVLFDVSSSADYGKLSKRDLR
                     EMIAGSRVEVAPYKKAPQDFVLWKPSAEDQPGWQSPWGRGRPGWHIECSAMAAKHLGQ
                     TVDIHAGGQDLVFPHHENELAQSTCAHDGRTLARYWLHNGYLSMDKEKMSKSLGNVLL
                     VHDLITIFPGEVLRLALLGAHYRQPLDWTDEAVDGARSRLDRLYGAVRGIELSDEDRL
                     RAEPPAAVVAALEDDINTPLALAEVASIARRLNKETDSGVRRELALQVYAACDLLGLM
                     QVGAEEWF"
     gene            1347..1823
                     /gene="hisI"
                     /locus_tag="VFY27_08665"
     CDS             1347..1823
                     /gene="hisI"
                     /locus_tag="VFY27_08665"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX5765626.1"
                     /translation="MSDEHIQFHARRSVDQVEEGTELAPKFDDRGLIAAVTTDAASGE
                     VLMQGYMNAEALRLTISTGEAHYFSRSRQSIWHKGASSGLFQEVQELLIDDDQDCVWL
                     RVVVKGGASCHVGYRSCFYRRIPLGDDHQGELVFTESAKVFDPEEVYGDAPNPTIL"
     gene            complement(1891..2589)
                     /locus_tag="VFY27_08670"
     CDS             complement(1891..2589)
                     /locus_tag="VFY27_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765627.1"
                     /translation="MALVRYISYFVGIALVTWLLTRMEINAPGNLRLQEFTYTGDTLG
                     TSEFSPLELLQPFMLIICGLLYAWVAWHYPSQRPIAFGFGGLALAFTIRELDFFLDRY
                     IVDNFWQVIVALVSALIITYTYRHRKRFRIAWLRLWPSPGLTLLFAGAVIQFAFVRLV
                     GHEPLWMAILGDDYLRIIKLAVEEFIELIGYFLWTIGTVEYVFQVRVIATREPLHVAA
                     KRRAGRQPKSKGRY"
     gene            complement(2726..3232)
                     /gene="lptE"
                     /locus_tag="VFY27_08675"
     CDS             complement(2726..3232)
                     /gene="lptE"
                     /locus_tag="VFY27_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5765628.1"
                     /translation="MSSHPGTLTVKVLLLIALLAASAGCGFQLRSNPVVPPEMARTYI
                     ATNDRYSLFYRTFRTQLEDAGAELVDSPVDATAEFAILTDDTGQRVLSVSARNVPTEF
                     EVYYTVHYTVRSGQKTLLEPQLQTLTRDYTYDETRVLGKAREEELLRTAIVHDLVRLV
                     LVQVSSIQ"
     gene            complement(3225..5675)
                     /gene="leuS"
                     /locus_tag="VFY27_08680"
     CDS             complement(3225..5675)
                     /gene="leuS"
                     /locus_tag="VFY27_08680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5765629.1"
                     /translation="MSRPYHPESVEKEARRYWEETRCFEVREDPRKEKFYSLTMFPYP
                     SGQLHMGHVRVFTINDVIARYQRMQGKHVLQPMGWDAFGMPAENAAIQRGIPPAKWTY
                     RNIDYMRGQLKRLGFGYDWSRELTTCRPEYYRWEQWLFTKLFEKGLVYRKNSVVNWDP
                     VDQTVLANEQVIDGRGWRSDALVERREIPQWFMKITAYADELLDNLDRMDGWPESVKT
                     MQRNWIGRSEGVELSFDVPGSEEPLPVFTTRPDTLMGVTYMAVAAEHPLAEKAAAQDE
                     KLAAFLKECKQTQAAEASLETMEKKGMPLGISAIHPLSGEEVPLWVANFVLMGYGTGA
                     VMAVPGHDERDWEFARKHGLRVVQVVRPADGSAVDVQENAFTDYGILVNSGEYDGMDY
                     RQAFDAIAERFEQSGHGRRSVHYRLRDWGVSRQRYWGTPIPIIYCDKCGAVPVPEEEL
                     PVVLPEDVEFTGVGSPLRQMPSFVNTRCPSCGAAARRETDTFDTFVESSWYFARYACP
                     DSHDAMLDEREGYWMPVDQYTGGVEHAILHLLYARFFQKLMRDFGLSSVDEPFTNLLT
                     QGMVLKDGAKMSKAKGNTVDPQQLIETFGADTVRLFMMFAAPPEQALEWSDEGVQGAF
                     RFLKRFWRAVQEHVSGGAGAIVDPAALNDAQKALRRKIHQTIAKVSDDVGRRYTFNTA
                     IAAVMELLNALNRLKDSSAAGRAVIHEGLEAVVLLLSPIVPHICHELWHALGHEEAIV
                     NVPWPKADASALVREMVEIVVQVNGKLRGRISVAAGSDTDTISAAALADENVKRFVAD
                     KPIRKTIVVPGRLVNVVV"
     gene            complement(5741..7243)
                     /gene="lnt"
                     /locus_tag="VFY27_08685"
     CDS             complement(5741..7243)
                     /gene="lnt"
                     /locus_tag="VFY27_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5765630.1"
                     /translation="MRPALLRLAFFAAGAMLTIAFAPLGFNALAPLVLLPLLSACVQA
                     RPRDAARLAFWFGAGLFLFGTYWLYTSIHVFGQAPLWVAILLMVGLVVIMGWYFAAIG
                     WLVTVLTGGNSWRLVLAAPAGWVAVEWFRGWFLTGFPWMSIGYSQVNSPLAGWLPVLG
                     VYGASFLVVLSASAVLLVLERAASRQRAGQWAAVAIAVSPWIVGAGLQWVQWTEPAGA
                     ARTTTIVQGGVSQDRKWLPEQFPVTLDLYRDSIARHPGSDLILWPEVAIPAAVDEVEG
                     YLELLQLDLEERGQILLLGILERPERDTIYNSVVMIDGGERQAYRKRHLVPFGEYFPV
                     PDWVRERMRLMSLPHTDMSRGEATQPLLETSGGDRLAAVICYEDAYGAEQLYALPEAT
                     ILINVSNDAWFGDSIAPHQHLEIARVRAAEAGRYVVRATNNGISAFIGPRGELLQTAP
                     QFQYAALTADVLPMRGATPYSVVGNWPVIGLTLLIVAGFGWSSYTRRNHR"
     gene            complement(7342..8202)
                     /locus_tag="VFY27_08690"
     CDS             complement(7342..8202)
                     /locus_tag="VFY27_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX5765631.1"
                     /translation="MNDKPPSTGGSGSTGVVKRIFRAIKGDPWSREEIHDLLQQAESV
                     FDPEEQNMLQGVLEVAETQVREVMIPRSQMVVIERSQKLDEMLKVIIESGHSRFPVIG
                     EDRDEVLGVLLAKDLLRYFNDEQRGELRIEDYLRPAAVIPESKRLNALLKEFRDSHNH
                     MAIVVDEYGGVSGLLTIEDVLEEIVGEIDDEHDREEAACIQPETDAEGRSTYLVRALT
                     RIEDFNEYFDTNLPDDDYDTTGGLVMHELGRLPRRGERLTFGGFEFRVLKADRHRIDT
                     LQVQREIQPE"
     gene            complement(8285..8755)
                     /gene="ybeY"
                     /locus_tag="VFY27_08695"
     CDS             complement(8285..8755)
                     /gene="ybeY"
                     /locus_tag="VFY27_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5765632.1"
                     /translation="MELQQACDVASVPGAAEIRRWIEAAIHAATSDRGDIGDCEIVVR
                     IVGEEESRALNSRYRHKDSATNVLAFPANEAAIPGLPAGPLPALGDLAICGPVVEREA
                     LEQGKPVASHWGHLLVHGTLHLLGYDHQSKAEAAEMERLEVRIMADCGFGDPYR"
     gene            complement(8822..>9334)
                     /locus_tag="VFY27_08700"
     CDS             complement(8822..>9334)
                     /locus_tag="VFY27_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019627165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5765633.1"
                     /translation="EVRRIILVRPAVEAGERLGFLPGDMSQKVDPYLRPMYDALFDMI
                     GPEQVARLIERSVIEIAPLAFMRGRSLNESFVILDVAQNTSVAQMKMFLTRIGFGSHA
                     VVTGDITQIDLPKGSESGLKNAIKILADVEGIAFTYFTPGDVVRHELVQRIVEAYEAD
                     GSGSKKPSNG"
BASE COUNT         1773 a         2904 c         2826 g         1831 t
ORIGIN      
        1 gaaccattct tcggcaccca cctgcatgag gccgagcagg tcgcaggcgg cgtacacctg
       61 cagcgcaagt tcccttcgga cgccgctgtc ggtttccttg ttcagccgcc gcgcgatgct
      121 tgcgacttcg gcgagggcaa gtggtgtatt gatgtcgtct tccagcgcgg caacgaccgc
      181 cgccggaggt tccgcgcgca acctgtcttc atcgctcaac tcgatgccgc ggacggcgcc
      241 gtacaggcgg tcgagcctcg agcgggcacc gtcgacggcc tcgtcggtcc agtcgagcgg
      301 ctgccggtaa tgggcaccca gtagcgcgag tcgcagtact tcgccgggga agatggtaat
      361 gaggtcgtgc accagcagga cgtttcccag cgacttcgac atcttctcct tgtccatgct
      421 gagatagccg ttgtgcagcc agtaacgcgc aagcgtccgg ccgtcatgcg cgcaggtgct
      481 ctgcgcgagc tcattttcgt ggtgggggaa gacgagatcc tgcccgccgg cgtggatgtc
      541 gacggtctgt cccaggtgtt tcgccgccat cgcggaacat tcgatatgcc agcccggcct
      601 tccgcgcccc cacggcgact gccagccagg ctggtcctcc gcggagggct tccacagcac
      661 gaagtcctgc ggcgccttct tgtatggtgc aacctctacg cgcgagccag caatcatttc
      721 ccgcaagtcg cgcttcgaca gtttcccgta gtcggcagat gaggagacat cgaacagcac
      781 gtgtccttcg gccgcgtacg cgcaaccgct atcgagcaac gtggcgatca tgtctatcat
      841 ctccgcgatg tgctcggtgg cgcgtggttc gatgtcgggg ggtttcacac caagcgctgc
      901 catgtcgctt ctgtaagcct cggcataccg ctcggttatg gctccgattg gctcgccggt
      961 ctctttcgcg gccgcgatga tcttatcgtc cacatcggtc aggttccgcg cgaagataag
     1021 cgtgaagttc cggcgcaaca gcctggcaag cacatcgaag accacggcgg ggcgtgcgtt
     1081 gcctatgtgt gcgtagttgt agacggttgg cccgcacagg tacatcgtca cgcggccggg
     1141 gaccaggggc tcgaacggga tcttcgctcc cgtcagggtg tcgtgtaagt gaatggccat
     1201 ggcaccctgt cgtcaggctg gtcgcaaatc gatccggaat ggttggtaat gatagcaaca
     1261 acgcccatgc catgaatccg ggcggcgtac ccgatgacct ataatggcgg ctcgcactaa
     1321 agccgggaag tcagcaggag cgaagaatgt ccgacgagca catccagttc cacgcccgcc
     1381 gctctgtcga ccaggtcgag gaaggtacgg aactggcgcc gaaattcgac gaccgcggcc
     1441 ttatcgcggc cgtaacgaca gacgccgcct ccggcgaggt gctgatgcag ggctatatga
     1501 acgcagaggc cctgaggctc accatttcga ccggtgaggc tcactacttc agccgcagcc
     1561 ggcaaagcat ctggcacaag ggcgcgtcca gcggactctt ccaggaggtg caggaactgc
     1621 tcatcgacga cgaccaggac tgtgtctggc tccgggtggt cgtaaaaggc ggggcgagtt
     1681 gccatgtcgg ttaccggtcc tgcttttacc ggcgcattcc cttgggcgac gatcaccagg
     1741 gcgagctggt tttcaccgaa agcgcgaagg ttttcgatcc cgaagaagtt tacggggatg
     1801 cgccgaaccc gacgatcctc tagccggtta gttcacaggc taaagacgag gccggaaagg
     1861 accctgcagg agcccgccat cgtttcggtg tcagtagcgg cctttcgatt ttggctgcct
     1921 tcccgcgcgg cgcttggccg cgacgtgcag tggttcgcgg gtggcgatga cacgcacctg
     1981 gaacacgtat tcgacagtgc caatggtcca gaggaaataa ccgatcagct cgataaactc
     2041 ctcgacggcg agcttgatga tgcgaaggta gtcgtcgccg aggatcgcca tccacaacgg
     2101 ctcatggccg acgaggcgga caaaggcaaa ctggattacg gcgccggcga agagcagggt
     2161 gagtccgggc gacggccaaa ggcgcagcca ggcgatacga aaacgttttc gatgccgata
     2221 cgtataggtg atgatcagag cggagaccag ggcgactatc acctgccaga agttgtcgac
     2281 tatgtatcgg tcgaggaaaa agtcgagctc gcgaatcgtg aatgcgagcg ccaggccgcc
     2341 gaaaccgaac gcgattggtc gctgcgacgg gtagtgccat gcgacccacg catacagcaa
     2401 gccgcagata ataagcatga aaggctggag gagctccagc ggcgagaact cgctcgtgcc
     2461 gagcgtgtcg cccgtatagg tgaattcctg caagcggagg ttgcctggcg cattgatctc
     2521 catccgcgtc agcagccagg taacgagtgc tatgccgacg aagtaactga tgtagcgaac
     2581 gagtgccatg cgccgccctt tgcttcacgg attggtacta tagcggtatt cccggtccag
     2641 ttcgcgtcag gagaggtatg caacctgcgg cgcgtgacga actctgcgtg gaaatagccg
     2701 aggtgtgcga ccgggccggc gaacgttact ggattgacga cacctgtacc agtaccaggc
     2761 gaaccaggtc gtggacgatt gccgtgcgca gcagttcttc ctcccgggct tttccgagca
     2821 cccttgtctc atcgtaagtg tagtcgcgtg taagcgtctg cagctgcggc tcgagcaagg
     2881 ttttctggcc gctcctcacg gtgtagtgaa ccgtgtaata cacctcgaac tctgtcggca
     2941 cgttgcgtgc ggacactgac agtacgcgct ggccggtgtc gtcggtgagt atggcaaact
     3001 cggcggtagc gtctacggga gagtcgacca gttcagcgcc cgcatcttcc agctgcgtgc
     3061 ggaacgtgcg gtagaaaagg ctgtacctgt cgttggtggc aatgtatgtg cgggccattt
     3121 cgggcggcac gacaggattg ctgcggagct ggaaaccgca gcctgcagat gccgcgagca
     3181 aggcaatcaa cagcaggact ttcactgtga gcgtacccgg gtggctagac aacgacgttt
     3241 accagtcgtc ccggcaccac gatggttttc cggatcggct tatcggcgac gaaacgcttc
     3301 acgttctcat cggcgagcgc tgctgcggaa atcgtatcgg tatctgaacc cgcggccact
     3361 gagatccggc cgcgtaactt gccgttcacc tgcacgacaa tttcaaccat ttcgcgtacg
     3421 agggccgatg cgtcggcttt cggccacggc acgtttacga ttgcctcctc gtgccccagc
     3481 gcgtgccaca gctcgtggca gatatgcggt acgatcggtg acagcagcag caccaccgct
     3541 tccagccctt cgtgtatgac cgcacggccc gcagcggaac tatccttcag ccggttgagc
     3601 gcgttgagca attccattac cgcagcgatc gccgtgttga aagtgtagcg ccgcccgacg
     3661 tcgtcgctga ctttcgcgat tgtttggtga atcttgcgcc ggagcgcttt ctgtgcgtcg
     3721 tttagcgcgg ccggatcgac tatcgcgccc gcaccgccgg agacgtgttc ctggacggcg
     3781 cgccagaagc gcttcaggaa acggaacgcg ccctgcactc cttcgtccga ccactcgagc
     3841 gcctgttccg gcggcgcggc gaacatcatg aacaggcgaa cagtatccgc accgaaagtt
     3901 tcgatgagct gttgcggatc gacagtgttg ccctttgcct tggacatttt cgcgccgtct
     3961 ttcagcacca tgccctgcgt cagcaggttt gtaaatggtt catccacaga cgatagcccg
     4021 aaatcgcgca tcagcttctg gaagaagcgc gcgtacagga gatgcaagat tgcgtgctcg
     4081 acgccgccgg tgtactggtc gaccggcatc cagtagccct cgcgctcgtc cagcattgca
     4141 tcgtgactat ctggacaagc gtagcgcgcg aagtaccacg acgattccac gaaggtatcg
     4201 aaggtatccg tttcgcgcct tgccgctgcc ccgcagcttg ggcaacgcgt gttcacgaac
     4261 gagggcatct gccgtagcgg cgagccgacg ccggtaaact ccacgtcctc cggcagcacg
     4321 accggaagct cctcctccgg caccggcacg gcgccgcact tgtcgcaata gatgatcgga
     4381 atgggcgtgc cccagtagcg ctggcgcgaa acgccccagt cgcgcaggcg gtagtgcacg
     4441 ctgcggcggc cgtggccgga ctgctcgaaa cgttcggcga tcgcgtcgaa cgcctggcgg
     4501 taatccatgc cgtcatactc gccggagttg accaggattc cgtaatcggt aaacgcattc
     4561 tcctggacat cgacagcact gccatcagcc ggccgcacca cctgcacgac acgcaagccg
     4621 tgtttcctcg caaattccca gtcacgctcg tcgtgtcccg gaaccgccat gacggcgccg
     4681 gtgccgtatc ccatcaacac gaagttcgcg acccagagcg gaacctcttc accgctgagc
     4741 ggatggatcg cggagatgcc cagcggcatt ccctttttct ccattgtctc gagactcgcc
     4801 tcggcagcct gcgtctgctt gcattccttc aggaatgccg ccagcttctc gtcctgcgcc
     4861 gctgcttttt ccgccagtgg gtgttcggcg gctacggcca tgtacgtcac gcccatgagc
     4921 gtgtcggggc gcgtcgtgaa gaccgggagc ggttcttcgc tgcccggcac gtcgaacgaa
     4981 agctccacgc cttccgagcg gccgatccag tttcgctgca tcgtcttcac ggactctggc
     5041 cagccatcca tacgatccag gttgtccagc agctcgtccg cgtaagccgt gatcttcatg
     5101 aaccactgcg ggatctcccg ccgctccacg agtgcgtcgg aacgccagcc acgcccgtcg
     5161 atcacctgct cgttcgccag taccgtctgg tcgaccggat cccagttgac caccgagttc
     5221 ttccggtaca ccagtccctt ttcgaacagt ttcgtaaaca gccactgttc ccagcgataa
     5281 tattcaggcc tgcaggttgt gagttcgcgg gaccagtcgt aaccgaagcc aagccgcttc
     5341 agctgtcccc gcatgtagtc gatattccgg taggtccatt tcgccggtgg aatgccgcgc
     5401 tggatggcag cattctcggc cggcataccg aacgcatccc aacccatcgg ctgcagcacg
     5461 tgctttccct gcatgcgctg gtagcgcgcg atcacgtcat tgatcgtgaa gacccggaca
     5521 tgccccatgt gaagctggcc gctcgggtaa gggaacatcg tcagggagta aaacttctcc
     5581 ttgcgcggat cctcgcgaac ttcgaagcag cgcgtttctt cccagtatct gcgggcttct
     5641 ttttcaacgc tctcggggtg atacggtctg gacatctggt acggtgttca atgcagggca
     5701 aaggcgggaa ggatatctgt ttcgccgtcg gcacgcagcc tcagcgatgg tttcgcctcg
     5761 tataagaaga ccagccgaag ccggcgacga tgagaagcgt aaggccgatc accggccagt
     5821 taccgacgac gctgtaggga gttgcgccgc gcatcggtag cacatccgcg gtcagtgcgg
     5881 cgtactggaa ctgcggcgcc gtctgcagca actcgccacg ggggcctatg aatgcgctga
     5941 tgccgttatt ggtcgcccgg acgacgtagc ggccggcttc ggcggcgcgt acccgggcga
     6001 tttccaggtg ctggtgcggc gcgatcgaat cgccaaacca ggcatcgtta ctgacgttga
     6061 tcaggatcgt ggcttccggc agcgcgtaga gctgctccgc tccgtaggcg tcttcgtagc
     6121 agatgactgc cgccaggcga tctcctccgg atgtttccag caacggctgc gtggcttcgc
     6181 ctctcgacat gtccgtatgt ggcaggctca tgagccgcat ccgttcgcgg acccagtccg
     6241 gaacgggaaa gtactcgccg aacggtacca ggtgacgctt gcggtatgcc tgccgctcac
     6301 ctccgtcgat cattacgaca ctgttgtaga tcgtgtcgcg ctccggacgc tccaggatcc
     6361 cgagcagcag tatctgccct cgctcctcga ggtccagctg cagcaattcc agatagcctt
     6421 cgacctcgtc gactgctgcc gggatcgcga cttcgggcca caggatcaaa tcgctgccgg
     6481 gatggcgcgc aatggaatcg cggtaaagat cgagcgtgac cggaaactgt tccggcagcc
     6541 acttcctgtc ctgggagacg cctccctgca cgatggtggt ggtgcgcgcc gctccggccg
     6601 gctccgtcca ctgcacccac tgcaaacccg ctccgacaat ccagggagac acggcaattg
     6661 caacggcagc ccattgcccg gcccgttgcc ggcttgccgc gcgctccagc acgagcaaca
     6721 cggcgctggc gctcaacacg acgagaaacg atgcgccgta gacgcccagc accggcagcc
     6781 agcccgccag cggcgaattt acctggctgt agccgatgct catccaggga aacccggtga
     6841 ggaaccagcc acggaaccat tcgacggcaa cccaaccggc cggcgcggcc aggacgagcc
     6901 gccatgagtt gccgccggtc agcacggtca ccagccagcc gatggcggca aagtaccagc
     6961 ccatgatgac cacgagcccc accatgagga gtatcgcgac ccacaggggt gcctgcccga
     7021 agacatgaat ggaggtgtag agccagtagg taccgaacag gaacaaccct gcgccaaacc
     7081 agaatgccag gcgtgcagca tcgcgcgggc gtgcctgcac gcatgccgac agaagcggca
     7141 ggagtacgag cggcgccagc gcgttgaacc cgagcggcgc gaaagcgatg gtcaacatgg
     7201 cgccggcagc gaagaatgcc agccggagca gggccggccg cacatccaag agaccctgga
     7261 gcgggtatgc gttgttagtg acgggaaaca ccagttcgtt gttcgtgcct gatgtcgcgt
     7321 tgcgatatca ggatcggaag gctactccgg ctggatctca cgctgcacct gcagcgtgtc
     7381 aatccgatgg cggtctgcct tgagcacgcg aaattcgaaa ccgccgaacg tcaatctctc
     7441 gccgcgcctc ggcaggcggc ccagttcgtg catcaccagc ccgccggtcg tatcgtaatc
     7501 gtcatccggc aggttcgtgt cgaaatattc gttgaagtcc tcgatcctcg tcagtgcccg
     7561 cacgaggtac gtggagcggc cctccgcatc ggtctcgggt tgtatgcacg ccgcttcctc
     7621 gcgatcgtgc tcatcgtcga tctcaccgac aatttcttcc agcacgtcct cgattgtcag
     7681 caggcctgag accccgccgt actcgtcgac gacgatcgcc atgtgattat ggctgtcgcg
     7741 aaactccttg agaagggcat tgagccgctt cgattccgga atgaccgcgg caggccgcag
     7801 gtaatcctcg atgcgcagct cgccgcgctg ctcatcattg aagtagcgca agaggtcctt
     7861 ggcgagaagt acgccaagta cttcatcgcg atcttcgccg atgaccggga agcgggagtg
     7921 accggattcg atgatgacct tgagcatctc gtcgagcttc tggctgcgtt ctatgacaac
     7981 catctgcgaa cgcgggatca tcacctcccg cacctgcgtt tcggcaacct cgaggacacc
     8041 ctgcagcatg ttctgctctt cggggtcgaa aacgctctcg gcctgctgta gtaaatcgtg
     8101 tatttcttcg cggctccagg ggtctccttt gatggcgcga aaaatgcgct ttacaactcc
     8161 ggtcgaccct gagccgccgg tacttggcgg tttgtcgttc attgaaaagg tgtggtctcc
     8221 tgacccgttt tggcggtcat tgtatcaggt gggaccggtg tcctgcgccg cgaagctcgc
     8281 ggggctaacg gtatgggtcg ccgaagccgc agtccgccat gattcgcact tccaggcgtt
     8341 ccatttcggc ggcctcggcc tttgactggt ggtcgtaccc caacaggtgc agtgttccgt
     8401 ggaccagcag gtgaccccag tggctggcta ccggcttccc ttgttccagg gcctcgcgtt
     8461 cgactaccgg cccgcagatt gcgaggtcgc caagcgccgg cagcggcccg gcgggaagcc
     8521 cgggaatggc ggcctcgttt gccgggaatg cgagaacgtt ggtggcactg tccttgtgcc
     8581 gatagcgact gttcagggcg cggctttcct cttcgcccac gattcttacg acgatctcgc
     8641 agtcgccgat atcgccacga tcgctggtgg cagcatggat cgctgcctcg atccagcgac
     8701 gaatttcagc ggctccggga acgcttgcga cgtcgcaggc ttgctgcagt tccacgttta
     8761 ccgttgcatt caaagcggtt gcaggtgtcg atgttgcgcg gaagaccggg gcgcgggcaa
     8821 cctaaccgtt tgagggcttt ttggagccgc taccgtcggc ctcgtacgcc tctacaattc
     8881 tctgcacgag ttcgtgtcga acgacatcgc cgggcgtgaa ataagtaaag gcgatcccct
     8941 ccacgtcggc aagaatcttg attgcattct tcaaaccgga ctccgaacct tttggcaggt
     9001 ctatctgggt gatgtcaccc gtcactaccg catgcgaacc gaaaccgatg cgggtcagga
     9061 acattttcat ctgcgcgaca ctggtgttct gcgccacgtc cagtatgaca aaagactcgt
     9121 tcagcgaacg gccccgcata aaggcaagcg gcgctatctc gataacactg cgctcgataa
     9181 gccttgcaac ctgctccggc ccgatcatgt cgaacaacgc gtcgtacatc gggcgaagat
     9241 acggatctac cttctgcgac atgtcgccgg gtaggaagcc caggcgctcg cccgcctcta
     9301 ccgccgggcg aacgagaatg atgcgccgca cttc
//
LOCUS       DASUOM010000418         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1276961,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000418 DASUOM010000000
VERSION     DASUOM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276961"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFY27_08705"
     CDS             complement(<1..425)
                     /locus_tag="VFY27_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5765634.1"
                     /translation="MEWFTALNVARLQFAFTVSFHIVFPAFTIGLASYLAVLEGLWLA
                     TGRNVYQRLFRFWVRIFAVAFGMGVVSGVVMSYQFGTNWSRFSDTTGPILGPLLGYEV
                     LSAFFLEAGFLGVMLFGMNRVGRGLHFFATVMVALGTLMS"
     gene            complement(464..769)
                     /locus_tag="VFY27_08710"
     CDS             complement(464..769)
                     /locus_tag="VFY27_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765635.1"
                     /translation="MSRADPPASRFVCSNEPFKIVEFARADPPSGQPESEWYRYVISQ
                     GRAAMAGMRQGSRESVIAAVEDIVSRMNERRLGRSGRVHLKMGSNPKAEQASGAKSG"
     gene            complement(866..1099)
                     /locus_tag="VFY27_08715"
     CDS             complement(866..1099)
                     /locus_tag="VFY27_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765636.1"
                     /translation="MTKEVIQDWTDSTVLLKFGKHRDVRYQVYRDGQRLLQEIRAEDD
                     TPIHTLELPTGLVMERQSYEVLLRYVLLDVVSN"
     gene            1380..2348
                     /locus_tag="VFY27_08720"
     CDS             1380..2348
                     /locus_tag="VFY27_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5765637.1"
                     /translation="MPDKPEGRTACCIGAMTMDRTLKLFEPGVLGTSNPVVSRRTRGG
                     VARNVAENLARLSVSCHLVSIVGDDEAGREVLKATAQEGVDTGLVQKSLSEPTGSCTR
                     VIQPDGALFASFADIGISKLMDRDFIHNRWLQISGATLVFAETNLPAETLSYLVTGCR
                     EHELTLVLDAVSVSKARRLPLNLNGVDMLFSNADEARAMLGDDIARDANGDGDTSSPE
                     ELQNMAQALCQRGARSAVVTGGTAGICFASDGRCILLPASPAAVSDVSGAGDALIAGT
                     LYGRLMDYDAVTSLTLGLNAAAMTVASHDNNAPDLSAEAITAGLDL"
     gene            2348..3274
                     /locus_tag="VFY27_08725"
     CDS             2348..3274
                     /locus_tag="VFY27_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX5765638.1"
                     /translation="MHLDIAADVADALAGGVPVIALETTVITHGMAFPHNVETALAME
                     DAILAERGCPATLGIVSGRVVVGMSKSQIEAFGRAEPGAIPKCSRRDIPAVVAAKLNG
                     SLTVAGTIAVADAAAIRMFATGGIGGVHRGHPHDVSADIYQLARTPVAGVCAGAKSIL
                     DLPLTLEVLESQGVAVLGLGTSNLPAFFTRDSGLPLPYSVETVDEAADVLRAWRDLEG
                     DSALLVTVPVPEDSALGPAEAERAVSQAVRDADERGISGNRLTPYILQRVVELTEGRS
                     LRANKALLLNNARIATLLARKAGLGRPDRDSR"
     gene            complement(3317..>4113)
                     /locus_tag="VFY27_08730"
     CDS             complement(3317..>4113)
                     /locus_tag="VFY27_08730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX5765639.1"
                     /translation="FTPKIGYPDIWKDYSTLDIDKDDLVGNMQRSALVVYQQQLDKQK
                     GPVQKHEWQMTPQTVNAYYNPPLNEIVFPAAILQPPFFDLHAEDAVNYGAIGAVIGHE
                     IGHGFDDSGSTFDGDGVLRDWWTKADKDEFKKRTARLVEQYSAFKPFDDINVNGEFTL
                     GENIGDLGGLSIALQAYKLSLGGREAPVMDGFTGEQRVFLGYAQAWRHKYREESLRTQ
                     IATDPHSPRMYRVNGVVRNVPEFYRAFDVREGDALYLAPSERVKIW"
BASE COUNT          750 a         1288 c         1284 g          791 t
ORIGIN      
        1 gacatcaggg tgccgagcgc caccatgacc gttgcgaaga aatggagccc gcgcccgacg
       61 cggttcatgc cgaacagcat cacgcccagg aagccggctt cgagaaagaa cgccgacagc
      121 acctcgtaac cgagcagcgg ccccaggatt ggaccggtcg tgtccgagaa gcggctccag
      181 ttcgtgccga actggtagct catgacgacg ccggaaacga cgcccatgcc gaaggctacg
      241 gcgaaaatcc gcacccagaa cctgaacagg cgctggtaga catttcgccc ggtcgccagc
      301 cacagccctt cgagtaccgc aaggtagctg gcgaggccga tggtgaacgc ggggaacacg
      361 atgtgaaaag acaccgtgaa ggcaaactgc aggcgggcga cgttaagggc ggtaaaccat
      421 tccatgtcgc cggttcaagc aggtgccgcc aatttttttg gcgttagccg gacttcgcac
      481 cggaagcctg ttccgccttt ggattgctgc ccatcttgag atgcacgcgg ccggaccgcc
      541 cgagcctgcg ctcgttcatg cggctcacta tatcttcgac cgcggcaatc acgctctcgc
      601 ggctgccctg gcgcatgccg gccatggcag cgcggccctg gctgatgaca taccggtacc
      661 attcggattc gggctgcccg gagggaggat cggccctagc gaattcgacg atcttgaagg
      721 gttcgttact gcaaacgaat ctgcttgcgg gtgggtctgc tctgctcatg ggggtccagc
      781 atacgctaat ccccctgcct ctgtgcgcaa atctggccag cgggacctgg catggcttgc
      841 cctcccgctc gttgcgtggt gccggtcagt tgcttacgac gtcgagcagg acatagcgca
      901 gcagaacctc gtaactctgg cgctccatca ccaggcccgt gggcagttca agggtgtgaa
      961 tgggcgtgtc gtcttcagcg cggatttcct gaaggagccg ctgaccgtcg cggtatacct
     1021 ggtaacgcac gtcccggtgc ttgccgaact tcagcagtac ggtgctgtcc gtccagtcct
     1081 ggatcacttc ctttgtcata gctgtcccct tcactgttcg cttgtttgcc gcggggcact
     1141 ccctccggtg ccgcctgcgc gcatgttgtc gacgatattg caagcgctgt gccatgttga
     1201 aactgttcga ggaaggatcg ataacctact gacatcgctc gttttacgat agtcctgttt
     1261 tcgccggcca gttccccgcg cttacgctcg ttgtcagctt ctgactgaag ttcatgcaat
     1321 tggcgacatt cgcttaacat aaacccatgg cgcacccgcc ctcctggaac ccgttcccta
     1381 tgcccgacaa acctgaaggc aggaccgcct gctgtatagg cgcgatgaca atggaccgca
     1441 ccctgaagct tttcgaaccg ggcgttctgg gtacctcgaa cccggtagtc agccgccgga
     1501 cacgcggcgg ggtcgcgcgc aacgtggcgg aaaacctggc gcgcctgtcg gtcagctgcc
     1561 accttgtgtc gattgtcggc gacgacgagg cagggcgaga agtcctgaaa gcgaccgcgc
     1621 aggaaggcgt cgacaccggg ctggtgcaga aaagcctgag cgaacccacc ggctcctgta
     1681 ccagggtgat tcagccggac ggcgcgctgt ttgcaagttt cgcggacatc ggcatctcga
     1741 agttgatgga ccgcgacttc attcacaatc gatggctgca gatatccggc gcgacgctgg
     1801 tgtttgccga aacaaacctc ccggcagaga cgctctccta cctggtcacg ggttgccgcg
     1861 agcacgagct gacgctggtg ctcgacgccg tatcggtgtc gaaagcgagg cggctgcctt
     1921 tgaacctgaa cggggtcgat atgctgttct ccaatgccga cgaggcgcgt gcgatgctgg
     1981 gagacgacat cgcgagagac gcaaacgggg atggcgatac gtcgagcccc gaagaactgc
     2041 agaacatggc gcaggcactt tgccagcggg gagcgagaag cgccgtcgtg acgggcggaa
     2101 ccgccggcat ctgtttcgcg agcgacggcc gatgtatcct gctgcccgcc tcgcctgctg
     2161 cggtaagtga cgtgtccggt gcgggcgatg ctcttatcgc cggtaccctc tacggccgcc
     2221 tgatggatta cgatgccgtg accagcctga ccctcggact gaacgccgcc gccatgacgg
     2281 tagcgagtca cgacaacaat gctccggacc tgtcggcaga ggcaatcacc gccggccttg
     2341 acctttgatg catctggata tcgcagccga tgtagcggac gcactggctg gcggcgtgcc
     2401 ggtaattgcc ctggagacca ccgtgatcac ccacggcatg gccttcccgc acaacgtgga
     2461 gactgccctt gccatggagg acgcgatcct cgcggagcgc ggttgtccgg cgacgctcgg
     2521 aatcgtcagc ggccgggtgg tggtcgggat gagcaagagc cagatcgagg cttttggcag
     2581 ggctgaaccc ggcgccatcc cgaagtgcag ccgccgcgat atccctgccg tggttgccgc
     2641 gaaactgaac ggttcgctga cggtagccgg caccatcgcc gtcgccgatg ccgccgcgat
     2701 ccgcatgttc gccaccggag ggatcggcgg cgtgcacagg ggccatccgc acgacgtatc
     2761 cgccgacatc taccagctcg cgcgcactcc tgttgcaggc gtgtgtgcgg gggccaagtc
     2821 gatcctggac ctgcccctga cgctcgaagt gctggagtcc cagggcgtcg ccgtgctggg
     2881 tctcggcaca tccaacctgc ccgcgttttt cacgcgtgac agcggcttgc ctctgcctta
     2941 ttccgtggaa accgtggacg aagccgccga tgtcctgcgc gcctggaggg acctggaggg
     3001 cgatagcgct cttctcgtca cggttccggt gccggaggat agcgcgcttg gcccggcaga
     3061 agccgaacgt gcggtcagcc aggctgtgcg ggatgccgat gaacgcggta ttagcggcaa
     3121 caggctgact ccttacatac tgcagcgcgt ggttgagttg accgagggcc gctcgctcag
     3181 ggcgaacaag gcactgctgc tgaacaacgc gaggattgcg acgctgctcg cgcgaaaggc
     3241 cggcctgggg cgtccggacc gggactctag gtaaaagtgg cgcccgtctc tgcgacggac
     3301 gccgcctcca ccggtcttac cagatcttca cgcgctccga cggggccagg taaagcgcgt
     3361 cgccctcccg cacgtcgaac gcccggtaga actccggcac gtttcgcacc acgccgttga
     3421 cacggtacat gcgcggggaa tgcggatcgg tggcgatctg cgtgcgcagc gattcctcgc
     3481 ggtacttgtg acgccaggcc tgtgcgtagc cgaggaaaac acgctgctca ccggtaaagc
     3541 cgtccatcac cggcgcttcc ctgccgccaa gggaaagctt gtacgcctgc aacgcgatgc
     3601 tgagtccacc gagatccccg atgttctcgc caagcgtaaa ttcgccgttc acgttgatgt
     3661 catcgaatgg cttgaatgct gagtactgct cgacgagcct tgcggtcctt ttcttgaact
     3721 catccttgtc ggctttggtc caccagtcgc gcaacacgcc atcgccatcg aaggtgctgc
     3781 ctgagtcatc gaagccgtgc ccgatttcat ggccgatcac cgcgccgatt gctccatagt
     3841 tgaccgcatc ttcggcgtgc aggtcgaaga acggcggctg caatatcgcg gcaggaaaca
     3901 cgatctcgtt gagcggcggg ttgtaatagg cgttgaccgt ttgcggcgtc atctgccact
     3961 catgcttctg caccggcccc ttctgcttgt cgagttgctg ctggtagacc accagcgcgg
     4021 aacgctgcat gttgccgacc aggtcgtcct tgtcgatgtc gagcgtcgag tagtctttcc
     4081 agatgtcggg atatccgatc ttcggcgtga act
//
LOCUS       DASUOM010000419         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1382922,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000419 DASUOM010000000
VERSION     DASUOM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382922"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..982
                     /gene="rrtA"
                     /locus_tag="VFY27_08735"
     CDS             359..982
                     /gene="rrtA"
                     /locus_tag="VFY27_08735"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEX5765640.1"
                     /translation="MGLHQASEVRNGSILRRSTAWVVPLLMTAISLLVSFAGTEGQSR
                     LRYERQDIAEGELWRLVTGHFAHLGLSHMLLNIAGLALVWLLVGGAWMLWQWAFITFI
                     SLAFIDLGFWFLDPGLNWYVGLSGLLHSMLVAGIVGRIRDAPGESLALGAIVLAKIAW
                     EQLAGPLPGSESSAGGAVVVNAHLYGAMAGIIAAGALRLRPAPRAPI"
     gene            1020..1952
                     /gene="fabD"
                     /locus_tag="VFY27_08740"
     CDS             1020..1952
                     /gene="fabD"
                     /locus_tag="VFY27_08740"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5765641.1"
                     /translation="MIFPGQGSQSVGMLAALAEQYPEVQATFAQASEILGFDLWQIAQ
                     EGPTKRLDETVITQPVMLAAGVATWRAWRSGGGATPSNMAGHSLGEYTALVCSGSLEF
                     ADAIRLVTRRAELMQAAVPPGEGAMAAIIGLDDDAVIAACDAAAQGQVVSAVNFNSPG
                     QVVIAGERAAVERATQHAKAAGAKRALLLNVSVPSHCELMRPAAEQLAQALAGTALAA
                     SSVPVTGNADVRTYEDAEQIREGLAKQLYSPVRWTETIRELVGRGSSSVVECGPGKVL
                     TGLVKRIDRSIPAVCIDTRETMTTALAEHAAPPN"
     gene            2022..2777
                     /gene="fabG"
                     /locus_tag="VFY27_08745"
     CDS             2022..2777
                     /gene="fabG"
                     /locus_tag="VFY27_08745"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5765642.1"
                     /translation="MTVTFNQDILNGRVALVTGASRGIGAAIAAALADSGAKVTGTAT
                     SADGARRISETLGDRGRGAVLDIADEESVQALFKELLDKEGAPTIVVNNAGITRDNLL
                     LRMKTEEWDDVLATNLSGAYRVCKASLRGMMKARYGRIINIASVIGVMGNPGQANYAA
                     AKAGIIGFSKSLAREVGSRMITVNVVAPGFIDTDMTRVLPEDQRQALLTQIPLGRLGQ
                     TSDIANAVTFLASDAAGYITGQTLHVNGGMLMA"
BASE COUNT          572 a          913 c          949 g          541 t
ORIGIN      
        1 ggagactacc ggcctgagga tatcgcgggc ggaagtgatg tccttgcaag cggcggcgag
       61 gtccgcgtac tgccgtttcg cgagggctac tcgtccacca gaataataga caagctgcgt
      121 tcgtaacggc ccatagtgcc ctggcccgca aataactacg cgatttcgct gcgtcttttc
      181 gcgtccggct cgggcatcct gcttcgctct accgcgtcca tccctggacg cgtgcgactc
      241 ctcgcaatag gcctgctatt ccccgtcgtc gcgccttgcc agacacaaaa atccgcgacc
      301 gaaactgtaa cgttatttgc gagacagcac accagcgccg cgcgtcagta taacaaacat
      361 gggcttacat caggcttccg aggtcaggaa tggttccatt ctgagacgca gtaccgcatg
      421 ggtggttccg ctcctgatga cggcaatttc gctcctggtg tcgttcgcgg gcacggaagg
      481 acaaagccgc ctgaggtacg aacgccagga cattgcggag ggcgaactgt ggcgtctcgt
      541 tacgggtcac ttcgcgcacc tcggcctctc gcacatgctg ctgaacatcg ccggactcgc
      601 gctggtctgg ctgctcgtag gcggtgcctg gatgctgtgg cagtgggctt tcattacctt
      661 catatcgctc gcgtttatcg acctcggctt ctggtttctc gaccctggac tgaactggta
      721 cgtcggcctc tccggcctgc tgcactcgat gctggtggcc ggcatcgtcg gccggatacg
      781 cgatgcgccc ggcgagtcgc tcgcgcttgg cgcgatcgta ctggcgaaga tagcctggga
      841 acagcttgcg ggaccgctgc ctggctccga aagctccgcc gggggtgcgg tggtcgtgaa
      901 tgcacacctc tatggcgcga tggcaggcat aatcgccgcc ggtgccttgc gcctccgtcc
      961 cgcgccgcgc gcgccgatat aatgttcttt tcccgtggag ccacgccgtg tcgcttgcca
     1021 tgattttccc gggccaggga tcgcagtctg tcggtatgct tgccgcgctg gcggagcagt
     1081 atccggaagt gcaggcaacg ttcgcgcaag catccgaaat cctgggcttc gacctgtggc
     1141 agatcgcgca ggagggcccg acgaagcggc tcgacgagac agtcatcacg caacccgtaa
     1201 tgctcgcggc aggggtggca acctggcggg cgtggcggag cggaggcgga gcaacaccct
     1261 ctaacatggc cggtcacagc cttggcgaat acaccgcact ggtctgctcg gggagcctcg
     1321 aattcgccga tgcgataaga ctggtcacgc gccgcgctga gctgatgcag gcggccgtgc
     1381 cgccgggaga gggggcgatg gccgcgatca tcggcctcga cgacgatgcc gtgattgcag
     1441 catgcgacgc cgctgcacag ggccaggtag tgagtgccgt aaatttcaat tcgcccggcc
     1501 aggttgtcat cgcaggcgaa cgcgctgcgg tcgaacgcgc gacgcaacac gcaaaagcgg
     1561 ccggagcaaa acgcgcactg ctcctgaacg taagcgttcc atcgcactgc gaactgatgc
     1621 gtcccgctgc cgaacaactg gcgcaagcac ttgccggcac cgcgctggct gcatcctcgg
     1681 tacccgtgac aggcaacgcg gacgtgcgga cttacgaaga tgcagagcaa atacgggaag
     1741 gcctcgcgaa gcagctctac agcccggtgc gctggacaga aactatccgc gaactcgtcg
     1801 gccgcggcag ttcctccgtc gtcgaatgtg gaccgggcaa ggttctcacc ggtctcgtga
     1861 agcgcatcga tcgaagcatc cctgccgttt gcatagatac ccgcgagacc atgaccaccg
     1921 cgcttgcgga acacgccgca cccccgaact aaccggcagc ggccatgccg ctgcgctcga
     1981 ttcaccaata tggattcagg aacgacaaag gagcccgacc gatgacggtt acgttcaacc
     2041 aggacatcct gaacgggcga gtggcgctcg taacaggagc atcgcgaggc ataggcgccg
     2101 cgattgccgc ggcgctggcg gactcgggtg cgaaggtgac aggcactgcc accagcgccg
     2161 acggcgcacg ccggatctcg gagacgctgg gcgaccgtgg ccgcggtgcg gtactcgata
     2221 tcgctgacga ggaatccgtg caggcgttgt tcaaagagct gctggataag gaaggcgcac
     2281 cgacgattgt cgtcaacaat gcgggcatta cccgcgacaa cctgctgctg cggatgaaga
     2341 cggaggagtg ggacgacgtg ctggcgacga atctttcggg ggcctacagg gtgtgcaagg
     2401 cgagcctgcg cggcatgatg aaggcgcgct acggaaggat catcaacatc gcgtcggtga
     2461 taggggtaat gggcaatccg ggccaggcga attacgcggc ggccaaggcc ggcatcatcg
     2521 gcttttcaaa atctcttgcg cgcgaggtcg gctcccggat gatcaccgtc aatgtggtcg
     2581 cgccgggttt catcgacacc gacatgaccc gcgtgttgcc ggaagaccag cgacaggcct
     2641 tgctgacgca gataccgctc ggcaggctgg gccaaacctc ggacattgcc aatgcagtga
     2701 cctttctcgc gtcggatgcc gcgggatata tcactggcca aaccctgcac gtaaacggtg
     2761 gcatgttgat ggcctgagac cagcggatgg tccgtttgcg aagcctgtcg gagtccttcc
     2821 ccgactcccc gaggcactcc gaacaggcgc aaacgcccgt cgttacgggg attgtagatt
     2881 gccacagggg tttcacatac aataccgtcc cgcggcgtga ctcgggactt gtgatatcaa
     2941 ggacttacat ctccggtgga gcgtcgcggc acgat
//
LOCUS       DASUOM010000420         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_437463,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000420 DASUOM010000000
VERSION     DASUOM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437463"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFY27_08750"
     CDS             <1..799
                     /locus_tag="VFY27_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5765643.1"
                     /translation="SPTILDNEARAKAEFGADVPMSEHPKIRSATACLASSSMAVDLA
                     RRHNARLHVLHLTTALEMGLFSRASRSEKSITAEVCVHHLWFDESRYEDLGTRIKCNP
                     AIKTARDREALVSAVNDGRIDIIATDHAPHTIAEKKRSYFKAPAGLPLVQHALQTLFD
                     LAKDGQISVETIVERTSHAVADIFGIVDRGYVREGYFADLVLIDSNKPCTVEASNLLY
                     KCHWSPFEGHTFSSTIDTTIVNGTVVFEDGHLAADIVGQRLQFTRGR"
     gene            1064..2878
                     /locus_tag="VFY27_08755"
     CDS             1064..2878
                     /locus_tag="VFY27_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5765644.1"
                     /translation="MKPTDTSKPDYFHKVVDCQWACPAHTDVPAYIRLIAQGRFSDAY
                     MLNRESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADGRGDITDRLPKA
                     PPVGNGKKVALIGAGCASLTVANDLLPIGYDVTIFEALGKTGGLMRTNIPQFRLPPKV
                     LDEEINYIVDMGADLKLNHRIDSMKALLDSREFDAVFVGTGAPRGKELELPGRYDTDR
                     IHIGIDWLESIAFEHTESIGKRVLIIGVGNTAMDCCRSALRLGAEDVKVMARKPRGFF
                     KASDWELEDAEEENVEILVNHSPKEFVVESGKLVGMKFDRMEYKVDESGRIDRGTPVG
                     EVLIPCDDVILAIGQDNAFPWIERDIGIEFNEWDSPVVDEKTMMCTREGVFFGGDAAF
                     GPKNIIWAVAHGHEAAISINKYCFAQDLEARLPYGLNLSSQKMGMHEWSFSNYYDPAE
                     RRLMPHVDLRERFKKIDIEVELGFTVEQTIKEVERCLNCDIQTVFEAKLCIECDACVD
                     ICPVDCLTITHNGEEDDLRTRLSAPAENRSQELYVSEPLPQTSRVMVKDEDICVHCSL
                     CAERCPTGAWDMQKSTILIPYAGDEAPQPVPRKIKATA"
     gene            2942..4744
                     /locus_tag="VFY27_08760"
     CDS             2942..4744
                     /locus_tag="VFY27_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5765645.1"
                     /translation="MSTVNDVVVKIATVNGTGSASANGLLMKSVFRMGVPVVGKNYFP
                     SNIQGLPTWYEVRVTKDGYRARADRVDVMVAMNAQTYAQDMAEVVSGGWLVYDSTWPR
                     SRLLDREDITVIGVPLSRMCNEHFEGARARILMKNIAYVGVLAALLEIDLDVITQLLE
                     ETFAKKKHLAKSNLEAIRLGYDYTMEHFPCPLPARVAPMDKTKGHVIIDGNTAAGLGC
                     VYAGATVGAWYPITPSTSLMDAYKAFCKQFRMDEASGGKKFCIVQAEDELAAIGVVLG
                     ASWNGARAFTPTSGPGISLMNEFIGFAYYAEVPAVIFDVQRTGPSTGMPTRTQQCDLM
                     SCAYASHGDTKHVLLFPANPEECFYQSLTAFDLADRLQTPVLVLADLDIGMNDWMCPE
                     LEWDENYRPDRGKLLTAEQLEGMEKFYRYLDSDGDGITYRTLPGEHPKGSFFTRGSGH
                     NKFGAYTEDSAQYKEVVDRLLVKWETARNLVPVPEVEYSKFNKRAILTLGSCDAAVKE
                     AMDRLKEEGAPLNYCRVKAFPFSDAVKEFIDRHERVYVVEQNRDAQLRSLLMLDIEAD
                     GRKLVPVLHYDGMPLAANFIVQKVQEDLAKGRAA"
     gene            4741..5796
                     /locus_tag="VFY27_08765"
     CDS             4741..5796
                     /locus_tag="VFY27_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX5765646.1"
                     /translation="MSYIPKPKVSHPKLPRNKLGLTSRDYEGTVSTLCAGCGHDSITA
                     AIIQAVFELGIEPHMLGKMSGIGCSSKTPAYFVSQSHGFNSVHGRMPSVTTGANAANK
                     DLIYIGVSGDGDSLSIGMGQFAHVIRRNLNMCYIIENNGVYGLTKGQYSASADIGTVA
                     KKGPANRQMPIDPVSTAIGLGATYIARSFSGDKQQMVPLIKGALTHKGFALLDIISPC
                     VTFNDHEGSTKSYAHTRQHYHHVIDTDFVPPAEEIRAEYEEGGAMPVQLHDGSKIVFR
                     KVDNNYDPTSRAAAFSYLRSSIRAGEIITGLLYIDEEMGDMHDLSGSVDRPLSKIPYE
                     ELNPGPEKLAKVMSRYR"
     gene            6043..>7115
                     /locus_tag="VFY27_08770"
     CDS             6043..>7115
                     /locus_tag="VFY27_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5765647.1"
                     /translation="MNKQRRRLLQASLLGSTAMIANGLPQASAAAQPRGRKSKPLRIL
                     TLGGTGFIGPHMVREALRRGHEVSLFNRGRTDDDMFPDLQTFLGDRDNNLDALKGHEW
                     DAVIDNSGYVPRHVADSARLLSGAASHYLFVSSISAYASFRVANHEDSPLATMPDESV
                     EEVTNETYGPMKALCEKMAANEFGEDRLTILRPTYICGPGDRTDRYTYWPVRTMRGGQ
                     MLWPGRPSDPIQIIDVRDLANFTIDCIEQRTTGTYNTVTPPGEFTMGDLLEDCVAVTA
                     AEMVPQWVSADFLSEHDALQYMPIWSSTEGEESKAALVSGARAFAKGMKNRPTRETAR
                     DTIEWWRTLPADRTATLRAGLSP"
BASE COUNT         1478 a         2240 c         2165 g         1232 t
ORIGIN      
        1 ttccccgaca attctcgaca acgaagccag ggcaaaggcg gagtttggcg ccgatgtgcc
       61 gatgtcggag cacccgaaga tccgatccgc caccgcctgc cttgcgtcat ccagcatggc
      121 cgtggatctc gcgcgccggc acaacgcgcg cctgcacgtt ctgcacctga ctaccgcgct
      181 ggagatgggt cttttctccc gcgccagtcg ttcggaaaaa agcatcaccg ccgaggtatg
      241 cgtccatcac ctctggttcg acgaaagtcg ctacgaagac ctgggcacgc gcatcaagtg
      301 caaccccgcg atcaagaccg cgagggaccg ggaagccctg gtgagcgccg tcaacgacgg
      361 tcgcatcgac atcatcgcaa ccgatcacgc gccgcacacg atcgccgaga agaaacgcag
      421 ctacttcaag gctcccgccg ggctgccgct ggtgcagcac gccctgcaga ccctgttcga
      481 tctcgccaaa gacggccaga tcagcgtcga aaccatcgtc gagcgcacca gccatgccgt
      541 cgccgacatc ttcggcattg tcgaccgcgg ctacgtccgt gaaggctact tcgccgacct
      601 cgtcctcatc gacagcaaca agccctgcac cgtcgaggct tccaacctgc tctacaagtg
      661 ccactggtcg cccttcgaag ggcacacctt ctcctccacg atcgacacca ccatcgtcaa
      721 cggcaccgtc gtcttcgagg acggccatct tgccgccgac atcgtggggc aacggctgca
      781 gttcacccgc ggccggtgag gcttgcgctc tcggaagggt cgtacctgct tttgaggcgt
      841 cggcatggcc ggcggggttg cctttgacgg aatggctccc tctccgtccc ctttacttcc
      901 gccaaagctg aaccgcggat gaacgaaagc agccgccgga ggggcgtccg ccatacagcg
      961 atatagctgt ttactttgcg gacgataccg ttatccttgc cgcctattgg gcgctagcgt
     1021 gcaccgcgga cgaaacgcac tgaccatgca gtgcgtctat cgaatgaagc ctacagacac
     1081 atccaagccg gattattttc acaaggtagt tgactgtcag tgggcgtgtc ccgcccacac
     1141 ggacgtgcct gcctatatcc gcctgatcgc gcagggccgg ttcagcgacg cttacatgct
     1201 gaaccgggaa tccaacgtgt tcccgggcat cctggggcgc gtttgcgacc gcccgtgcga
     1261 accggcgtgc cggcgcggac gcgtcgagga aaagccggtg gcaatctgcc gcctgaagcg
     1321 ggttgcggcg gacgggcggg gcgacataac ggaccgcctg ccgaaggcgc cgccggtcgg
     1381 caacggcaag aaggtggcgc tgatcggcgc cggttgcgcg tcgctgaccg tggcgaacga
     1441 cctgctgccg atcggttacg acgtcaccat cttcgaagcc ctgggcaaga ccggcgggct
     1501 gatgcgcacc aacatcccgc agttccggct gccgccgaag gtcctcgacg aggagatcaa
     1561 ctacatcgtc gacatgggcg cggatttgaa gctcaaccac cgcatcgaca gcatgaaggc
     1621 gctgctggac agccgggagt tcgacgcggt gttcgtcggc accggcgcgc cgcgcggcaa
     1681 ggaactcgag ttgccgggcc gctacgacac cgaccgcatt cacatcggca tcgactggct
     1741 ggagtccatc gcgttcgagc acacggagtc gatcggcaag cgagtcctga tcatcggcgt
     1801 cggcaatact gcgatggact gctgccgcag tgcattgagg ctcggcgccg aggacgtgaa
     1861 agtcatggcg cggaaaccgc gtggcttttt caaggcttcg gactgggaac tggaagacgc
     1921 ggaagaggag aacgtcgaga tcctcgtcaa ccattcgccg aaggagtttg tcgtcgaaag
     1981 cggcaagctc gtcggcatga agttcgacag gatggagtac aaggtcgacg agagcggccg
     2041 catcgaccgc ggcacgccgg tcggcgaggt gctgattccc tgcgacgacg tcatcctcgc
     2101 gatcgggcag gacaatgctt tcccgtggat cgaacgcgac atcggcatcg agttcaacga
     2161 gtgggattcg ccggtcgtgg acgagaaaac catgatgtgc acgcgcgagg gcgtgttctt
     2221 cggcggcgac gctgcgttcg gtccgaagaa catcatctgg gcagtagcgc acggtcacga
     2281 ggctgcgatc tccatcaaca agtactgctt cgcacaagat ctcgaggcac ggctgccgta
     2341 tggcctgaac ctgtccagcc agaaaatggg catgcacgag tggagcttct cgaactatta
     2401 cgatccagcg gaacggcgac tgatgccgca cgtcgacctg cgggagcgct tcaagaaaat
     2461 cgatatcgag gtggaactcg gatttaccgt tgagcagacc atcaaggaag tcgagcgctg
     2521 cctgaactgt gatatccaga cggtctttga agcgaaactc tgcatcgaat gcgatgcctg
     2581 cgtcgatatc tgcccggttg attgcctgac gatcacgcat aacggcgagg aagacgacct
     2641 gcggacgcgg ctgtcggcgc ccgccgagaa tcgaagccag gaactctacg tatcggagcc
     2701 cctgccgcag acaagtcgcg taatggtcaa ggacgaggac atctgcgtcc attgcagctt
     2761 gtgtgccgaa cgttgcccga cgggcgcctg ggacatgcag aagtcgacca tcctcattcc
     2821 ctatgccggg gacgaggcgc ctcagcccgt accgcggaag atcaaggcga ccgcctgaag
     2881 gccatgattt tttgccccga tagctgtcta aagcttcgcc ttgaaccagg aaagatgctt
     2941 cgtgtccacg gttaacgacg tcgttgtaaa aattgccacg gtgaacggca ccgggtcggc
     3001 gagcgcgaac ggcttgctga tgaaatcggt tttcaggatg ggcgtgcccg tcgtcggcaa
     3061 gaattatttc ccgtcgaaca tccagggact gccgacctgg tacgaggtcc gcgtgacgaa
     3121 ggacggctac cgcgcccggg ccgaccgcgt ggacgtcatg gtggcgatga acgcacagac
     3181 ctacgcgcag gacatggcgg aggtggtgtc cggcggctgg ctggtatacg actcgacctg
     3241 gccgcgcagc cgcctgctcg accgcgaaga catcaccgtg atcggcgttc ccctttccag
     3301 gatgtgcaac gagcacttcg aaggcgcccg cgcccgcatc ctgatgaaga acatcgccta
     3361 cgtcggcgtg ctggcggcat tgctcgagat cgacctcgac gtcataacgc agttgctcga
     3421 agaaacgttt gcgaagaaga aacacctggc gaaatcgaac ctggaggcga ttcgcctcgg
     3481 gtacgactac acgatggaac attttccctg tccgctgccg gccagggtcg cgccgatgga
     3541 caagacgaag ggccacgtca ttatcgacgg caataccgcc gcgggccttg gctgcgtcta
     3601 tgccggcgcg acggtcgggg cgtggtaccc gattaccccg tctacttcgc tgatggatgc
     3661 ctacaaggct ttctgcaaac aatttcgcat ggacgaggcg agcggcggca agaaattctg
     3721 catcgtccag gccgaagatg aactggcggc tatcggtgtc gtgctgggag cttcgtggaa
     3781 cggcgcgagg gcgttcacac ctaccagcgg ccctggcatc tccctgatga acgagttcat
     3841 cgggtttgcc tactacgccg aggtaccggc cgtgattttc gacgtgcagc ggaccgggcc
     3901 atctaccggt atgccgacgc gaacgcagca gtgcgacctg atgtcctgcg cctacgcatc
     3961 acatggcgac accaagcacg tgctgctgtt ccccgcgaac ccggaggaat gtttctacca
     4021 gtcgttgacc gcgttcgacc tggcggaccg gctgcagacg ccggtgctgg tgctggccga
     4081 tctcgacatc ggcatgaacg actggatgtg cccggagctc gagtgggacg agaattaccg
     4141 gccagaccgg ggcaagctcc tgacggcgga gcaactggag ggcatggaga agttctaccg
     4201 ctatctcgac agcgatggcg acggcattac gtaccgtacc ctgcccggcg agcatccgaa
     4261 aggatcgttt ttcacccgcg gctccgggca caacaagttc ggcgcctata ccgaagattc
     4321 cgctcagtac aaggaggtcg tggaccgact cctggtgaag tgggagacgg cgcggaacct
     4381 ggtgccggtg cccgaggtcg aatactcgaa gttcaacaag cgcgccatcc tgaccctcgg
     4441 cagctgtgat gccgccgtga aagaagccat ggaccggctg aaggaggaag gcgcgccgtt
     4501 gaactactgt cgcgtgaaag ccttcccctt cagtgatgcg gtcaaggaat tcatcgaccg
     4561 gcacgagcgc gtgtatgtcg tggagcagaa ccgcgacgca cagctccgct cgttgctgat
     4621 gctcgacata gaggcggacg gacgcaaact ggtgccggta ctgcactacg acggcatgcc
     4681 gctggcggcg aatttcatcg tgcagaaggt gcaggaagac cttgccaagg gacgcgcagc
     4741 atgagctata tcccgaaacc gaaggtttca cacccgaagc tgcctcgcaa caagctgggg
     4801 ctgacgtcgc gggactacga gggcactgtc tccacgctgt gcgcaggatg cgggcatgac
     4861 tccatcacgg cggccatcat ccaggcagtg ttcgaacttg gcatcgagcc gcacatgctc
     4921 gggaaaatga gcggcatcgg ctgttcgtcg aagaccccgg cgtatttcgt cagccagtcg
     4981 cacggtttca attccgtgca cgggcgcatg ccgtcggtca cgaccggggc caacgcggcg
     5041 aacaaggacc tgatttacat tggcgtttcc ggcgacggcg actcgctttc gatcggcatg
     5101 ggccagtttg cacatgtcat tcgccgcaac ctcaacatgt gctacatcat cgagaacaac
     5161 ggcgtgtacg ggctgaccaa gggccagtat tccgcctcgg cggatatcgg tacggtggcg
     5221 aagaagggcc cggcgaaccg ccagatgccg atcgatcccg tcagcaccgc gatcggtctc
     5281 ggcgctacct acattgcgcg cagtttctcc ggcgacaagc agcagatggt gccgctgatc
     5341 aagggggctc tcacgcacaa gggatttgcg ctgctcgaca tcatcagccc ctgcgtgaca
     5401 ttcaacgatc acgaaggctc gaccaagagc tacgcgcaca cgcgacagca ttaccaccac
     5461 gtgatagata cggatttcgt gccgccggct gaagagatcc gcgccgaata cgaggagggc
     5521 ggcgccatgc cggtgcaact ccacgacggc agcaagatcg tgttccgcaa ggtcgacaac
     5581 aactacgacc cgaccagccg ggccgctgca ttttcgtacc tgcggagcag cataagagca
     5641 ggcgaaatca tcacgggcct gttgtacatc gacgaggaga tgggcgatat gcacgatctc
     5701 tccggcagcg tcgaccgccc cttgtcgaag atcccctacg aggaactgaa tcccggcccc
     5761 gagaagctcg caaaggtaat gagccgctac cggtaggtgg tgcgccgact gcgccctctg
     5821 aacacaccgc tgtaggagcc cgccgtgcgg gcgagttagc aaccctgtga gcaaccccga
     5881 tctcaaaata gagcactgca ggagcccgtc ccacgggcga ccctgcatcc ttaaagctaa
     5941 ggatcgcccc gccgccatat cacaattgca tctttccccc cgcctgccgt ccgtcgcctt
     6001 ctccgctaga atcgatgctc gaccaccgct tcggaccaca caatgaacaa acaaagacga
     6061 agactcctcc aggcaagcct cctaggctcc accgcaatga tcgccaacgg cctgccccag
     6121 gcatccgcag ctgcgcagcc tcgcgggcgc aaaagcaaac cgctaaggat cctcaccctc
     6181 ggcggcaccg gctttatcgg gccgcacatg gtacgcgagg cgctgcggcg cggtcacgag
     6241 gtgagcctgt tcaatcgcgg ccgcaccgac gatgacatgt tcccggacct gcagaccttc
     6301 ctgggcgacc gcgacaacaa cctcgatgcg ctgaagggcc acgagtggga cgcggtaatc
     6361 gacaactccg gctatgtgcc tcgccacgtc gcggattccg cacgcttgct gtcgggcgcg
     6421 gcgtcgcact acctgttcgt gtcttctatc tcggcttacg caagcttccg cgtcgcaaac
     6481 cacgaggact cgccgctcgc aaccatgccg gatgaatccg tcgaggaagt caccaacgag
     6541 acctacggtc cgatgaaggc attgtgcgag aaaatggccg cgaacgagtt tggcgaggat
     6601 cgcctgacga tcctgcgtcc cacctacatc tgcggcccgg gcgatcgcac cgaccgttat
     6661 acctattggc ccgtacgcac catgcgcggc ggccaaatgc tgtggcccgg acgaccgtcc
     6721 gacccgatac agatcattga cgtccgggat ctcgcaaatt tcacgattga ctgtatcgaa
     6781 cagcgcacca ccggaaccta caacacggtg acgccgccgg gcgaattcac aatgggcgat
     6841 cttctggaag attgcgtcgc ggttaccgcg gcggagatgg tacctcagtg ggtgagcgca
     6901 gacttcctga gcgaacatga cgccctgcaa tacatgccga tctggtcctc gaccgaaggc
     6961 gaggaatcga aagcggcgct ggtcagcggc gcgcgcgcgt tcgccaaggg catgaagaac
     7021 cgtccgaccc gcgagactgc tcgcgacacc atcgagtggt ggagaaccct gcccgccgac
     7081 cgcaccgcca ccctccgcgc cggcctgagc ccgga
//
LOCUS       DASUOM010000421         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_621940,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000421 DASUOM010000000
VERSION     DASUOM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621940"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFY27_08775"
     CDS             complement(<1..470)
                     /locus_tag="VFY27_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5765648.1"
                     /translation="MTKESYTLLSPDGASSLELPVRKGTLGPDVLDISHLYREQGVFT
                     YDPGFVATGSCESAITYIDGDEGVLLYRGYPVVQLAERSNFIEVAFLLLYGELPTSEE
                     LSKFDTSIRTHTMLNEGMLRFFNGFRYDAHPMAMLSAVVGSMSAYYHDTMDVNYP"
     gene            complement(700..906)
                     /gene="rpmE"
                     /locus_tag="VFY27_08780"
     CDS             complement(700..906)
                     /gene="rpmE"
                     /locus_tag="VFY27_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5765649.1"
                     /translation="MKAEIHPAYREIKVTCGCGNEFTTRSTLDHDLHVEVCSACHPFY
                     TGKQKIVDSGGRVDKFRRKYGISE"
     gene            complement(1353..>2512)
                     /gene="hemW"
                     /locus_tag="VFY27_08785"
     CDS             complement(1353..>2512)
                     /gene="hemW"
                     /locus_tag="VFY27_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5765650.1"
                     /translation="ASEAADKKRYVNALVKDIQLEGARAAGRVVESVFFGGGTPSYFD
                     AGEIGRVVEAIAGSCTLSDDAEVTMEANPGTVERDRLGDYRRAGVNRLSLGAQSFNDE
                     SLRRLGRIHGSEEVRNAWDDAVRAGFESINIDLMYALPGQTPEMARSDIEQVIALAPQ
                     HVSWYQLTLEPNTVFHARPPADLPDEDLASAIEEQGYELLGSAGYEHYETSAFAQPGH
                     CCRHNLNYWQFGDYLAAGAGAHGKFTDDRGGVWRYRKPAHPASYMQRVESGAPYDPMQ
                     RLLPSDLAFEFMLNALRLARGFASTQFTSRTGLPWDVVIPGVARAESLDLLCCGSGTD
                     GGYLIVPTARGRRFLNDLQAIFLPEAIPEGRAGSADAAKRTSGSIAAVPND"
BASE COUNT          481 a          758 c          775 g          498 t
ORIGIN      
        1 ggataattca catccatcgt atcgtggtaa taagcggaca tcgatccgac caccgcggat
       61 agcatcgcca tcggatgggc gtcgtagcgg aagccgttga agaaccgcag catcccctcg
      121 ttcagcatcg tgtgcgtgcg aatcgacgta tcgaacttcg acagctcttc gctggtcggg
      181 agctcgccgt ataaaaggag aaaggcgact tctatgaagt tggagcgctc cgcgagctgt
      241 accacggggt agccgcgata cagaagcaca ccctcgtccc cgtcgatata ggtaatcgcg
      301 ctttcgcagc tgccggtcgc aacgaagccg gggtcgtagg taaacacgcc ctgctcgcga
      361 tacaggtggc tgatatcgag cacgtccggc cccaaggtgc ccttgcgaac gggaagctcc
      421 agggacgagg cgccgtcggg gcttaggagc gtgtacgatt ctttggtcat agatttcccg
      481 ggacagtgag ccgcagcgat gtccggcgaa cggtgaaccc gcacgccgaa catcgtccgt
      541 atacgacgag ttagaagttt cgctcaagtt tggcggcgga gccggcgagt ccggcgtgcc
      601 gtcagtccgc catgttgccg gaagcgggaa acgcgagcaa atccacgcga gtaaaacgca
      661 ccgccgcagc ggctgcagtc gtcctgctgc ccggaaggcc tactcgctga tgccgtactt
      721 gcggcggaat ttgtctacgc ggccgccgga atccactatt ttctgcttgc cggtataaaa
      781 aggatggcat gccgaacaga cctcgacgtg gaggtcgtgg tccagggtcg agcgggtcgt
      841 gaattcgtta ccgcagccac aagtgacttt gatttccctg taggcggggt gtatctcggc
      901 cttcattagg tttgatctcc ggaaatttgg ccggtgtcat ttgcgtcgcc ccggccctgg
      961 caccgcctga agaacgcctc cgcgagaagg cggtgcggaa aaggcggcgt aggatatcgg
     1021 gatcacccgg cctccgcaag ggcgctcccg ccggacttcc gacgcgtggg aaagtatcca
     1081 caaaagctgt ggataactct gtggagtaaa attgagtgaa tgagctaagt cgggattttt
     1141 aaagcctcat tgttagaatg tctattttat gtgcaaaatg taaattctag gcctttcaat
     1201 atgttgctgg ctgttgtcga cagcgcaact gaattagctg gcttattctg cggaaaatac
     1261 agactaaggc ctttgttgtg aataaaatca ccttcccgtg gcctctcaac tgccggccat
     1321 ggtcgcgcca ccccaggagg gcctcccgca gatcagtcat tcgggacggc ggcgatgctc
     1381 ccggaggtcc ttttggcggc atcggccgag ccggcgcggc cttcgggaat ggcctccggg
     1441 agaaaaatgg cctgtagatc gttcaggaat ctccgcccgc gggccgtggg cacgatgagg
     1501 taaccgccgt cggtcccgga gccgcaacag agcaggtcca gcgactcggc acgggcaact
     1561 cccgggatta ccacgtccca tggcagcccc gtacggctgg tgaattgtgt gctggcgaag
     1621 ccgcgcgcga ggcgcaaagc gttgagcatg aactcgaagg ccagatcgct gggaagaagc
     1681 ctctgcatgg ggtcgtaagg ggcgcccgac tcgacccgct gcatgtaaga ggcagggtgg
     1741 gccggcttcc ggtagcgcca cacgccgccc cggtcgtcgg tgaacttgcc gtgcgcgccg
     1801 gcgccggccg ccaggtaatc gccgaactgc cagtaattga ggttgtggcg gcaacaatgt
     1861 cccggttgcg cgaacgccga cgtttcatag tgttcgtatc ccgcggagcc cagcagttcg
     1921 taaccctgtt cttctattgc tgaggcgaga tcctcatccg gcaggtccgc gggcggcctg
     1981 gcatggaaca cggtattggg ctccagcgtg agctggtacc acgagacatg ctgcggcgcg
     2041 agtgcgatga cctgctcgat gtcagagcgc gccatctctg gcgtctgccc tggcaacgcg
     2101 tacatcaggt caatattgat gctttcgaaa cccgccctta ccgcgtcgtc ccacgcgttc
     2161 ctgacttctt ccgagccgtg gatgcgcccc aggcggcgta gcgactcgtc gttgaagctc
     2221 tgggccccca gcgacaacct gttgacgccg gcacgccggt agtctcccag gcgatcccgc
     2281 tcgaccgtgc ccgggtttgc ctccatggtg acctcggcgt cgtccgatag cgtgcaggac
     2341 ccggcgatcg cttcgaccac ccggccgatc tcgccggcgt cgaagtaact cggcgtcccg
     2401 ccgccaaaga acacgctctc gaccacccgc ccggctgctc gcgcaccttc cagttggatg
     2461 tcctttacca gggcgttcac gtaacgcttt ttatcagccg cctcgctcgc cg
//
LOCUS       DASUOM010000422         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_671736,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000422 DASUOM010000000
VERSION     DASUOM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671736"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFY27_08790"
     CDS             <1..356
                     /locus_tag="VFY27_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5765651.1"
                     /translation="HGLDTMVGDRGVLLSGGQRQRIAIGRALLKDAPVLILDEATSAL
                     DSQAERHIQEALHALMRDRTTLVIAHRLSTVETADRIIVLDRGRIVESGTHKELLDQD
                     GRYAHLYRMQFNEEA"
     gene            379..1407
                     /gene="lpxK"
                     /locus_tag="VFY27_08795"
     CDS             379..1407
                     /gene="lpxK"
                     /locus_tag="VFY27_08795"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5765652.1"
                     /translation="MSGFINRVWYGDSPWYRVLLPLSWLFARLASLRRRLYRSGVLRS
                     FDVGKPVIVIGNIASGGTGKTPVTMWLASFLKHRGFRPGIVSRGYGGSVGRFPLQVSD
                     DSAAGVVGDEPLLMARRVICPVVVHPDRVAAASMLIEMGCDVVVADDGLQHYRLRRQF
                     EIAVVDGARGIGNGRLFPAGPLREPVSRLESVDLVMVQGADVDLAPILPRGVPVSTFE
                     LAVVAVHNLRGKRTVAIEEFRGKTVHGVAAIGNPQRFFRMLESYGMTVLPHGFPDHAL
                     LDDEDLDFGDGLDVLMTEKDAVKIGRVVPPHWWYIAVDLKLAHASGPHWLDELESALK
                     KIEGEQKA"
     gene            1404..2156
                     /gene="kdsB"
                     /locus_tag="VFY27_08800"
     CDS             1404..2156
                     /gene="kdsB"
                     /locus_tag="VFY27_08800"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5765653.1"
                     /translation="MSNFAVVIPARYASERLPGKPLRKIAGKDLLQHVFERATESDAD
                     EVVVATDDARIEQAARKFGADVCMTDPAHRSGTERLAEVARLRGWADDLVVVNLQGDE
                     PMMPPALINQCASLLQSRDVVMATLASPVASAEDFDNPNVVKVVLDANQDAIYFSRAA
                     IPHPRSSSMKDTAIKAALHHHGIYAYRVATLQQIVAAAPSQLERIEQLEQLRALEMGI
                     RIRVGRPASRPGPGVDTEADLLRVEQLILAGA"
     gene            2287..>3559
                     /gene="zwf"
                     /locus_tag="VFY27_08805"
     CDS             2287..>3559
                     /gene="zwf"
                     /locus_tag="VFY27_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5765654.1"
                     /translation="MEKPESDAVVIFGITGDLAYKKIIPALANLKRRGRLGMPFIGVA
                     REGWTLERLRERITASLRENGDERCESTGQELAESLQYVAGDYREAGTFRRLREALGD
                     AQTPLHYLAIPPSLFGEVIGSLDESGCSRGARVVVEKPLGRDLRSARELNRTLANFFD
                     ESSIFRIDHYLGKEPVQNLLYFRFANSFLEPLWNRVHVSSVQFTMAESFGIEGRGKLY
                     EELGAIRDVVQNHLLQVVSLLAMEPPVRYASDSLRDEKVKVLQAVRPLKPANVVRGQY
                     EGYRDEADVAAGSCVETYAALRVDIDSARWAGVPFFIRAGKCMAATATEVMVRFHPPA
                     HRLFSTRTGTPNYVRFRLGPDRMAIGLGVHIKEPGEAMSGRASELLMCDEEAGRMGAY
                     ERLIGDALRGDPTLFARQDSIEEAWRIVDEVI"
BASE COUNT          663 a         1053 c         1172 g          671 t
ORIGIN      
        1 ctcatggtct cgataccatg gtgggtgatc gcggagtact tctgtcggga gggcagaggc
       61 agcgcatcgc gattggtcgc gccctgctga aggatgcccc cgtgctgatt ctcgacgaag
      121 ccacctcggc actggattca caggccgagc gccacataca ggaggcgctg catgcactga
      181 tgcgagaccg gacgacgctg gtcatagcgc accggctgtc gaccgtggag accgcggaca
      241 ggatcatcgt gctggaccgt ggccgcatcg tggaatccgg cacgcacaag gaactcctcg
      301 atcaggatgg tcgctatgcg cacctttatc gcatgcagtt caacgaggag gcctagtccg
      361 aactaggaac ttgcaccagt gagtggcttc atcaaccggg tctggtacgg cgattcgcca
      421 tggtaccggg tgttgctgcc gctgagctgg ctgttcgcgc gactggcgtc gttgcggcgc
      481 cgcctgtacc gttcgggagt gctgcgctcg ttcgatgtcg gcaaaccggt aatcgtcatc
      541 ggcaacatcg cgagcggcgg caccggcaag acgccggtca cgatgtggct tgcttccttc
      601 ctgaagcaca ggggattccg gcccggcatt gtgagccgcg gctacggcgg gtcggtcggc
      661 aggttcccgt tgcaggtctc ggacgactcc gctgcaggag tcgtcggtga cgaaccgctc
      721 ctgatggccc gccgggtgat ttgtcctgtc gtcgttcacc ctgaccgtgt tgccgctgca
      781 tcgatgctca tcgagatggg ctgcgacgtt gtcgttgcgg acgacggcct gcagcattac
      841 cgcctgagaa ggcagttcga gatcgccgtt gtcgatggtg cgaggggtat cggcaacggc
      901 cgcctttttc ctgcggggcc cctccgggaa cccgtgtcgc ggctcgaatc ggtcgacctg
      961 gtcatggtgc agggcgccga tgttgacctg gcgccaatcc tgccgcgcgg agtgcctgtt
     1021 tccacattcg agctggcggt cgtggcggtg cacaacctgc gggggaagag gacggtcgcg
     1081 atcgaggagt ttcgcggtaa aaccgtgcat ggcgtcgctg caatcgggaa cccgcagcgg
     1141 ttcttcagga tgcttgaatc gtacggcatg actgtcttgc cgcatggatt tccggatcac
     1201 gcgctccttg acgatgaaga cctggacttc ggcgacgggc tcgatgtgct aatgacggag
     1261 aaagacgctg tcaaaatcgg cagggtggtt cctccgcatt ggtggtacat cgccgttgac
     1321 cttaaactcg cccacgcgag cggaccgcac tggctggatg aactcgagtc ggcattgaag
     1381 aaaatagaag gcgaacagaa ggcatgagca atttcgcggt ggtgatacct gcgcgctatg
     1441 catctgaaag gctccctggc aagcctctgc gaaagatcgc cggaaaggac ctgctgcagc
     1501 acgtcttcga gcgcgcgacg gaaagcgacg ccgacgaggt cgtggtcgcg acagatgacg
     1561 ctcgcattga acaggcggcg aggaaatttg gcgccgatgt ctgcatgacc gacccggccc
     1621 accgatccgg gacggagagg ctggcggagg tggctcgatt gcgcggctgg gccgacgacc
     1681 tggtggtggt aaacctgcag ggggacgagc cgatgatgcc tcctgccctg atcaaccagt
     1741 gcgcgtcact gctgcaatca cgcgacgtcg tcatggctac actggcgtcg ccggtcgcct
     1801 cggcggagga cttcgacaac cccaacgtcg tcaaggtcgt ccttgatgca aaccaggatg
     1861 cgatctattt cagccgcgct gcgataccgc atccgagaag ctcttccatg aaggacacag
     1921 ccattaaagc ggcgctgcat catcacggga tctatgcata ccgcgtggcg accctgcagc
     1981 agattgttgc tgccgcacct tcgcaactcg aacggatcga gcaactcgag cagctgcgcg
     2041 ccctcgaaat gggcatacgg atccgggtcg ggaggccggc cagtcgtccg ggtcctggcg
     2101 tcgacacgga agcggacctc ttgcgcgtcg agcagctcat tctcgccggc gcttgaaggc
     2161 agcaggcaaa agagggaaag tcaggctaca ggggcaacta gggagcccct gcacaagtca
     2221 tggacggcgt ttttttgccg tccagacttg tgcagagact ccccaggccc gcaaccggat
     2281 ccacgcgtgg agaagcccga atccgacgcc gtggtgatct tcggcatcac cggcgatctc
     2341 gcctacaaga aaatcattcc cgcgctggcg aacctgaaac ggcgcggccg cctcggcatg
     2401 ccctttatcg gtgtggcccg cgaaggctgg acgctggaac gattgcggga gcgaatcact
     2461 gcaagcctca gggaaaatgg cgatgagcgc tgcgagtcga cggggcagga gctcgctgaa
     2521 agccttcagt acgtcgccgg ggactaccgc gaagcgggca ccttccggcg attgcgcgag
     2581 gcattgggcg acgcacagac gccgttgcat tacctcgcca tcccgcccag cctttttggc
     2641 gaagtgatcg gtagtctcga cgagtcgggt tgcagccgcg gcgcccgcgt tgtcgtggag
     2701 aagcccttgg gccgtgacct gcgctccgct cgcgaactca accggacgct cgccaacttt
     2761 ttcgacgagt cgagtatctt tcgcatcgat cactaccttg gcaaggagcc ggtgcaaaac
     2821 ctgctgtatt tccgcttcgc caacagtttc ctggagcccc tgtggaaccg ggtgcacgtg
     2881 tccagcgtgc agttcacgat ggccgagtct ttcggcattg aaggccgcgg caagttgtac
     2941 gaggaacttg gcgcaattcg cgacgtggtg cagaatcacc tcctgcaggt ggtgagcctg
     3001 ctcgcgatgg aaccgccggt gcgttatgcg agcgactcgc tgcgcgacga gaaagtcaag
     3061 gttctgcagg ccgtgcgccc cttgaaaccc gcgaatgtgg tacggggcca gtacgagggg
     3121 taccgtgacg aagcggacgt cgccgccggc tcctgcgtcg aaacctacgc cgccctgcgc
     3181 gtggacatcg attctgcgcg ctgggcagga gtgcctttct tcatccgtgc aggcaaatgt
     3241 atggctgcga ccgcgacgga ggttatggtc aggttccacc ctccggcgca ccggttgttt
     3301 tcgaccagga ccggcacgcc gaattacgtg cgatttcgac tcggcccgga ccggatggcc
     3361 atcggactcg gcgtgcacat caaggagccc ggcgaagcaa tgagcggccg cgcgagtgaa
     3421 ctgctgatgt gcgacgagga ggctggcagg atgggcgcat acgaacgttt gattggcgat
     3481 gcgctgcgcg gcgatccgac tttgttcgcg cggcaggatt cgatcgagga agcctggcgc
     3541 atcgtcgatg aggtaatcc
//
LOCUS       DASUOM010000423         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1493639,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000423 DASUOM010000000
VERSION     DASUOM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493639"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFY27_08810"
     CDS             <1..1118
                     /locus_tag="VFY27_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765655.1"
                     /translation="FRIAPIFLQRYRDIFASLYKEDRALTVRRETWGLFLGLVGTAAF
                     YGVYGWIAIATMQQVVSLGEMTMYVMVFKQGQSAVSASLSAIGGMYEDNLYLSNLYEY
                     LEQRGAEDSGTVTRGDRPGDGVRFEEVGFRYPGSPRPALTGIDLHLLPGQSLALVGEN
                     GAGKTTLIKLLTGLYRPDTGRILLDGTPLAEWDTEVLRRRIGVIFQDFARYQLSVGEN
                     IGVGDVAHFHDTRRWQEAAGQGMADAFIAELPARYETQLGSWFKDGQELSAGQWQKIA
                     LSRAFMDRDADILVLDEPTASMDAAAEAALFEHFRKLTEGKITILISHRFSTVRRADH
                     IVVIENGRIIERGTHEELMRMQGRYATLFSLQAEGYR"
     gene            1324..2319
                     /locus_tag="VFY27_08815"
     CDS             1324..2319
                     /locus_tag="VFY27_08815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5765656.1"
                     /translation="MRKVPVEAILPLALSTVAVVGVLPFAFIRIANGDWMMAFVDSII
                     IMGFAFLGFSVLRPRRVRFASLSISILCVVGVLTTVYIRGEQQIFWAYPALLATFYLA
                     KPGEAVLISTLTIIALVPALVPKMGGIMLTTTFVTLIATSVFAYAFAFLSRGQRDQLL
                     QLARKDPLTGAGNRRALDEKLMEVCAAQARANTPASLVLIDIDNFKEINDEFGHAVGD
                     QILVRLTEIIELRIRVTDSLYRIGGEEFVVVIEGQTKDKARRMAEQLRTLVEANELAP
                     EGSVTISLGVAELAIGESPDDWIRRADLALYESKRCGRNQTSLAATGKILQAERA"
BASE COUNT          485 a          739 c          770 g          512 t
ORIGIN      
        1 tgttccgcat cgcgccgatt ttcctgcagc ggtatcgaga catctttgca tcgctttaca
       61 aagaggaccg ggcgctcaca gttcggaggg aaacatgggg cctgttcctc ggactcgtgg
      121 gaaccgccgc gttctatggc gtttacgggt ggatcgccat tgccacgatg cagcaggttg
      181 tttccctcgg cgaaatgacg atgtacgtga tggtattcaa gcaggggcaa tcggcggtat
      241 cggcaagcct ttccgcgatc ggcgggatgt acgaggacaa tctttacctg tcgaacctct
      301 atgagtatct cgagcagcgc ggcgcggaag acagcggcac cgtaacccgg ggagaccggc
      361 ccggcgacgg tgtgcgcttc gaggaggtcg gtttccggta tcccggcagc ccccggcctg
      421 cgcttaccgg catagacctc cacctgctgc ccgggcagag tttggcgctg gtgggcgaga
      481 acggcgccgg caagacgacc ctgataaaac tgcttaccgg gctgtaccgc ccggacactg
      541 gccgaatcct gctggacggg acgccgcttg ctgaatggga taccgaagtc ctgcggcggc
      601 gaatcggcgt catcttccag gatttcgccc gctaccagtt gagtgtcggg gagaacatcg
      661 gcgtagggga cgttgcgcat ttccacgata cccgccgttg gcaggaggca gcgggccagg
      721 gcatggctga cgcctttatc gcggaattgc ccgcccgcta tgaaacccag ctcggcagct
      781 ggttcaagga cgggcaggaa ctgtctgccg ggcagtggca gaaaatcgcc ttgtcacgcg
      841 cgttcatgga ccgggacgcg gatatcctcg tgctcgacga gcccaccgcg tctatggatg
      901 ctgctgccga ggccgcgctc ttcgaacact tccgcaagct gaccgaaggc aagatcacga
      961 tactgatttc ccatcgtttc tccacagtgc ggcgggcgga tcacatcgtg gtcatcgaga
     1021 acggccgcat catcgagcgc ggcacccacg aggagctgat gcgaatgcag gggcgctacg
     1081 cgaccctctt cagcctgcag gccgaaggtt accggtagga tcccaaggag gacacgaccg
     1141 gcccgaacgg gccatatgca aaaactgtga caaactatcc tgaatggcta ttcgtcccgt
     1201 ctatagaatg tgctcgacat ctcgtccgga gacgtgagaa aaacgacgtt ttcgcctttt
     1261 ttcggatcga ttatggtcgt tgacgaacat gcgtaggttt gccctcgccg aaaggccttg
     1321 aaaatgcgca aggtcccggt tgaagccatt ctgcctcttg ccctcagcac ggttgccgtc
     1381 gtgggggtgc tgccgtttgc cttcatccgc atcgccaatg gcgactggat gatggcgttc
     1441 gtcgattcaa tcatcatcat gggttttgcg tttctcggct tttcggttct acgtccccgc
     1501 cgggtgcgct tcgccagcct ctcgatttcg atcctgtgcg tcgtgggcgt gttgaccacg
     1561 gtctatatcc ggggcgaaca gcagattttc tgggcctacc cggcgctact cgcgacgttc
     1621 tacctggcga agccgggtga agccgtgctg atctcgacgc tgaccatcat cgcgctcgtt
     1681 cccgcgctgg tcccgaaaat gggcggcatc atgttgacca ctacgttcgt cacgttgata
     1741 gctaccagtg ttttcgcgta cgcgtttgcc ttcctgtcgc gcggtcagcg cgaccagcta
     1801 ctgcagctgg cacgcaagga tccattgacc ggtgccggta accggcgggc gctggacgag
     1861 aagctgatgg aagtatgtgc cgcccaggcg cgcgccaata cgcctgcctc gctggtgctc
     1921 atcgacatcg ataacttcaa ggagatcaac gacgagttcg gccatgcggt tggcgaccag
     1981 atactcgtgc ggctcactga aatcatcgaa cttcggatac gcgtcacgga cagtctctac
     2041 cgcatcggcg gcgaggaatt cgtcgtggtt atcgaggggc agacgaaaga caaggcacgg
     2101 cgcatggcgg agcaactccg cacgctggtg gaagccaacg aactggcgcc cgaaggctct
     2161 gtcaccatct cactcggtgt cgccgaactt gccatcggcg agtctccgga tgactggatt
     2221 cgccgcgccg acctcgctct ctatgaatcc aaacgctgcg gccgcaacca gaccagcctc
     2281 gctgccaccg ggaagatcct gcaggccgaa cgggcctgat aagcacctgc cgacaaagtt
     2341 gcgaacacgc gaatatgtgc tgtcacgcat ggtcggaacc gttgcgaggt ggagatgtga
     2401 ttgggaactt tccccgggga ctggctgcct taattaggca gaactgaccg gtattgcccg
     2461 gaagggcgct acggaggtag ctgcgctgca tgaagaagct gaattt
//
LOCUS       DASUOM010000424         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1244250,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000424 DASUOM010000000
VERSION     DASUOM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244250"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VFY27_08820"
     CDS             complement(<1..1111)
                     /locus_tag="VFY27_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5765657.1"
                     /translation="MSELPRFATLDPFSGMTAENPGTVRNLLHGKWMPAHAARDDVVD
                     PMNGAPFLQVPDTREHHDFIESVRRCPKTGLHNPLKNPQRYVMLGRVCAAAAALLAQH
                     AVQDYFTKLIQRVMPKDWQQCQGEVLITRVFLENFSGDGVRFLARGFSNPGDHLGQES
                     HGYRWPFGPVAVIAPFNFPLEIPALQVMGALFMGNKPLVKVDSKVSAVFEQFLRLLIH
                     CGLPAEDVDLIHCRGSEMGKLLDAARGDLKLVQFTGSSEVAERIAKQFNGAVRLEDAG
                     FDWKVLGPDYVPRWLEYVAWQCDQDAYNATGQKCSAQSILFMHENWSPHLLPKLKELA
                     ARRKLDDLTLGPVLSWTTDSMLAHIDAVKSIDGSRL"
     gene            1312..1408
                     /gene="ffs"
                     /locus_tag="VFY27_08825"
     ncRNA           1312..1408
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFY27_08825"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1501..>2279
                     /gene="dnaX"
                     /locus_tag="VFY27_08830"
     CDS             1501..>2279
                     /gene="dnaX"
                     /locus_tag="VFY27_08830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5765658.1"
                     /translation="MSYLVLARKWRPKNFRETSGQEHVLKALTNALETERLHHAYLFT
                     GTRGVGKTTIARILAKSLNCEANGITADPCGQCGACREIDEGRFVDLIEVDAASKTKV
                     DDTRDLLDNVQYAPARGRFKIYLIDEVHMLSRSSFNALLKTLEEPPPHVKFLLATTDP
                     QKLPATVLSRCLQFNLKRLTPRLIRERLEYICGEEGIASDVGSLAMIARAADGSLRDA
                     LSLLDQAIAYCGGELQQEAVATMLGTIDREHVSAVLGLLADG"
BASE COUNT          420 a          699 c          695 g          465 t
ORIGIN      
        1 ggagcctgga accgtctatc gacttcaccg cgtctatgtg ggccagcatg ctatcggtgg
       61 tccagctgag taccggtccc agcgtcaggt cgtccagctt gcgccgcgct gcaagttcct
      121 tgagctttgg cagcaggtgc ggcgaccagt tctcgtgcat gaacaggatg ctctgcgcgg
      181 agcatttctg cccggtggcg ttgtacgcgt cctgatcgca ctgccaggcc acgtactcca
      241 gccaccgcgg gacgtagtcg ggaccgagga ccttccagtc gaagcctgca tcctcgagcc
      301 tgacggcgcc attgaactgt tttgcgatgc gttccgcaac ttcactcgaa cccgtgaact
      361 gaacgagttt caggtcgccg cgcgccgcat cgagcagttt gcccatctcg ctgccccggc
      421 agtgaatcag gtcgacgtcc tccgccggca atccgcagtg aatcagtaac cggaggaact
      481 gctcgaatac ggcgcttact ttggagtcaa ccttcacgag cggcttgttg cccatgaaaa
      541 gcgcgcccat gacctgcagc gcggggattt ccagcggaaa gttgaacggg gcgatgactg
      601 caacggggcc gaacggccag cggtagccgt gggattcctg ccccaggtgg tctccgggat
      661 tggaaaagcc gcgggcgagg aacctgacgc cgtctccgga aaaattttcg agaaagaccc
      721 gcgttatcag cacttcgccc tggcactgct gccagtcctt aggcatgacg cgctggatga
      781 gtttcgtaaa gtagtcctgc accgcgtgct gcgccagcag cgccgctgca gcagcgcaca
      841 ccctgccgag cattacgtat cgctgtggat tcttcaatgg attgtgcagt ccggttttgg
      901 ggcagcgtcg tacgctctct atgaagtcgt ggtgttcgcg cgtatccggt acctggagga
      961 acggtgcgcc gttcattgga tccaccacat cgtcccgcgc cgcgtgggct ggcatccatt
     1021 taccgtgcag gaggttccgc accgtacccg gattttccgc ggtcatcccc gaaaacgggt
     1081 cgagcgtcgc gaatcgtgga agctcgctca tcgatcactc cagttgcgcc gcgggcgcct
     1141 tttccggcgg ctttagggac agaggcgaag aataacaagc tccgcgcgcc ttttctcatg
     1201 gccttggttc tcggtacgaa ctttgagcgc cgccaagtgc ttgtttcggt gcgggtcgcc
     1261 gcggacgcat tgagtatgcg aaccgctaag gcgataatta ctcccggtcc gcgtcggttt
     1321 ccccgcgacg gtcagccgtg aaccccgtca ggcccggaag ggagcagcgg cagcggtcat
     1381 accgggtgcc ggggaggtgc tggcgcgggc caacttgata ttctttttga acttcccccg
     1441 cttgtgcagt gtggccggcc attggttaca ctctgatctc ttttcagtgc cgctgcccct
     1501 atgagctacc tcgttctcgc ccgcaaatgg cgaccgaaga attttcgcga aacgtcagga
     1561 caggaacatg tcctcaaggc gctgacgaac gcacttgaaa cggaacggct gcatcacgcg
     1621 taccttttta ccggcacgcg tggcgtcggg aagacgacga tcgcgcgcat cctcgcaaag
     1681 tcactgaatt gcgaggccaa cggcattact gccgatccct gcggccagtg cggtgcctgc
     1741 cgggaaatcg acgaaggtcg ctttgtcgat cttatcgagg tagacgctgc atcgaagacc
     1801 aaggtagacg atacccgcga tttgctggac aacgtgcagt acgcgccggc gcgggggcgt
     1861 ttcaaaatct acctgatcga cgaagtgcac atgctgtcgc gaagttcctt caacgcgctg
     1921 ctgaagactc ttgaagagcc gccgccacac gtcaaatttt tgctggcaac gactgacccg
     1981 cagaaactgc ccgctacggt cctctcgcgc tgcctgcagt tcaacctgaa gcggttgacg
     2041 ccgaggctga tccgcgagcg cctcgagtac atttgcggcg aggaaggcat tgcgtccgat
     2101 gtcggctcgc tcgcgatgat cgcccgcgcc gcggacggca gcctccgtga cgcgttgagc
     2161 ttgctcgacc aggctatcgc ctactgcggc ggtgagttgc agcaggaagc agtcgcgacc
     2221 atgctcggga caatcgatcg cgagcacgtg agcgccgttc tcggcctcct ggcggacgg
//
LOCUS       DASUOM010000425         4996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_574470,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000425 DASUOM010000000
VERSION     DASUOM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574470"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..732)
                     /locus_tag="VFY27_08835"
     CDS             complement(43..732)
                     /locus_tag="VFY27_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5765659.1"
                     /translation="MADGRNLSQGGVFFDLDGTLVDTAPDMVAVLVAMQISNGSIPVP
                     YELARASVSHGAMGLINLAFPSVGEVQRSQLHQEFLDRYEQAVCVDSALFPGMDELLH
                     LLEGRGRPWGVVTNKPERMTDPLLEKLELKQRMSCAVSGDTLPQRKPHPAPLLLACEL
                     AGVAPERSIFVGDDARDIVAGRAAGMATIAAAYGYITEEDDPVLWEADQVAADTMELT
                     RMICDAMKLYS"
     gene            complement(735..1469)
                     /gene="ubiG"
                     /locus_tag="VFY27_08840"
     CDS             complement(735..1469)
                     /gene="ubiG"
                     /locus_tag="VFY27_08840"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5765660.1"
                     /translation="MKQTTQHDAARSDQNVDPAEIAKFDSLASRWWDPTGDFRPLHEI
                     NPLRLGYIRQSTELRDKRVLDIGCGGGILTESMAAAGARVTGIDMAESPLAVARLHQA
                     ETGSAIEYLQVPAEDHAAERAAEYDVVTCLEMLEHVPRPSTVVSACAALVRPGGDVFF
                     STINRNPKAFLFAIVGAEYLLRLLPAGTHEYSKLIRPSELEAWGRHAGLKLEDSIGMH
                     YNPILRQYSLGPNIDVNYLMHFQRPE"
     gene            complement(1492..2820)
                     /locus_tag="VFY27_08845"
     CDS             complement(1492..2820)
                     /locus_tag="VFY27_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX5765661.1"
                     /translation="MQSCDTLVMPRWCVPVVPADATLTETAVAISEGRIIDILPTADA
                     REKYQAREIVERPDHVLIPGLINAHTHAAMTLFRGIADDMPLERWLTDGICPSEKRWA
                     SAEMVRDGTRHAIAEMLRGGITCFSDQYFFPEIVAATASELHMRAVIGTPVIDFATAW
                     AENASQCISKGSELVHDRYADDPLITSCFAPHSTYVVPDEAFHELRIVADQLDARVQI
                     HLHETAREVEDAERATGKRPLQRLADLGLVNSSLMAVHAVHMTQKEVQLLADSGVAVA
                     HCPRSNLKLASGIADVPAMRAAGVVVALGTDGAASNNVLDMLGEMRTAALLAKVKAAN
                     AAVLTAKEVLRMATLDSASALGVADSTGSIERGKWADLTCISLQSVNSQPVYDPLSQL
                     VYTSHPDQVSDVWVAGRRQVDSGRLVAIDEADVLRRSAEWQERIAARIGT"
     gene            3098..>4996
                     /gene="gyrA"
                     /locus_tag="VFY27_08850"
     CDS             3098..>4996
                     /gene="gyrA"
                     /locus_tag="VFY27_08850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5765662.1"
                     /translation="MAGIAQEILSVSLEDEMQQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLHAMRELGNDYNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYML
                     VDGQGNFGSVDGDMPAAMRYTEVRMSRIAHEILSDLDKETVDFTENYDGTESEPSVMP
                     TRIPNLLVNGSSGIAVGMATNIPPHNLTEIVNAALALIDDPAIETAALMEHVPGPDFP
                     TAGIINGAQGIYSAYRTGRGRVHIRARTNMETIDKRGREAIIVTELPYQVNKARLIEK
                     IADLVREKRIEGISELRDESDKDGMRVVIELRRGENSDVVLNNLFKQTPLQSVFGINM
                     VALHEGQPKLMNLKQVLEAFLSHRREVVTRRTIFDLRKARDRAHVLEGQSVALANIDE
                     VIALIKASPSPVEAKQGLMQRNWSPGAVTGMLERAGATDTRPDGLDPGFGLADGQYRL
                     SEVQAQAILDLRLHRLTGLEQDKIFKEYQDILLRIKELSRVLEDPDELLRVIRAELLE
                     VRESYGDARRTEIIKDHTDLSLEDLILPEEVVVTLSHGGYAKAQPIDVYQAQRRGGRG
                     RSAAKVKDEDFIDKLFVANTHDTLLCFSSRGKMYWLKVYQVPQASRGSRGKPIVNLLP
                     LEAGERINA"
BASE COUNT          992 a         1496 c         1545 g          963 t
ORIGIN      
        1 ggaaagggga ggcgaatgga agctcgagcg gcagggacgg catcaggaat agagcttcat
       61 ggcgtcgcag atcatcctgg tcagttccat cgtatcggcg gcgacctggt ccgcttccca
      121 gagcaccggg tcatcctcct ccgtgatata gccgtaggct gcggcgatcg tcgccatgcc
      181 cgcggcgcgg ccggcaacga tgtccctggc atcgtccccc acgaaaatgc ttcgctccgg
      241 cgcgacccct gcaagttcgc aggcgagcag caaaggcgcc ggatgcggct ttcgctgtgg
      301 cagcgtgtcg ccgcttacgg cgcaggacat cctttgctta agttcaagct tctcgagcag
      361 cgggtcggtc atgcgctccg gcttgttagt gacaacgccc cacggacggc ccctgccctc
      421 gagcagatgc agcaactcgt ccatcccggg aaaaagcgca gagtcgacgc acacggcctg
      481 ttcatagcga tccaggaatt cctggtgcag ttggctcctt tgtacctcgc cgacggacgg
      541 gaaggccagg ttgatcaggc ccattgcacc gtgtgagacg cttgcgcgag ccagctcgta
      601 tggcacggga atgctgccgt tgcttatttg catggcgacc aggacggcaa ccatgtcggg
      661 ggcggtatcg acgagcgttc cgtcgaggtc aaagaaaact ccgccctggc tcaggttcct
      721 gccatcagcc atcgtcattc gggacgctgg aagtgcatca ggtaattcac gtcaatattc
      781 gggccgagcg agtattggcg gagaatcggg ttgtaatgca tgccgatgct gtcctcgagt
      841 ttcaggccgg cgtgccgtcc ccacgcctcc agctccgagg gccggatgag cttgctgtac
      901 tcgtgcgttc ccgccggcag aaggcgcagc aggtattcgg cacctacaat agcaaacagg
      961 aacgctttgg ggttgcgatt gatcgtggaa aagaaaacat cgccgccggg acgaaccagc
     1021 gcagcgcagg cagagacaac ggtggacggc cgtggcacgt gctcgagcat ttcgaggcag
     1081 gtaacgacat cgtattccgc cgcgcgttcc gcagcgtgat cctctgcagg gacctgcagg
     1141 tactcgatag cgcttccggt ctccgcctgg tggaggcggg caacggccag gggactctca
     1201 gccatgtcga tacccgtcac ccgggcaccc gccgcggcca ttgattccgt gaggatgccg
     1261 ccaccgcagc ctatatccag cacgcgcttg tcgcgcagct cggtactctg ccttatgtag
     1321 ccgagccgca gtggattgat ctcgtgcaag gggcgaaagt cgcccgtggg atcccaccac
     1381 ctggaagcaa gcgaatcgaa tttcgcaatc tcggccgggt ccacattctg gtcagaacgg
     1441 gcggcatcgt gttgcgttgt ttgtttcact tcgagaaggt gcactagtga ttcaagttcc
     1501 gattctcgcg gcgatgcgtt cctgccattc cgcgcttctc ctgaggacgt cggcctcgtc
     1561 gattgcgacc agccgtccgc tgtcgacctg gcgtctgcct gcgacccaga cgtcgctgac
     1621 ctgatccgga tggctagtgt atacgagttg cgacagcgga tcgtacacgg gttggctgtt
     1681 gaccgactgc aggctaatac aggtcaggtc cgcccattta ccccgctcga tcgatcccgt
     1741 gctatcggct actccgagcg cgctcgcgga atcgagcgtc gccatccgca agacctcctt
     1801 cgcggtcagc accgcggcat ttgcggcttt gactttcgcg agtaacgctg cagtccgcat
     1861 ttcgcccagc atatccaata cgttgttgct ggcggcaccg tcggtgccga gtgcgacgac
     1921 gactccggca gcgcgcatgg ccgggacatc ggcgatgccg ctcgcgagct tcaggttcga
     1981 gcgagggcaa tgagcaaccg cgacgccgga gtccgccagc agctgaactt ccttctgcgt
     2041 catgtgtacg gcatgtaccg ccatcagcga ggagttgacg agcccgagat cggcgagtct
     2101 ctgcaacggc cgcttgccgg tagcgcgttc tgcatcctcc acttcccgtg cggtctcatg
     2161 caaatgtatc tggacgcgtg catcaagctg gtcggcaacg atcctgagtt catgaaacgc
     2221 ctcgtccgga accacgtatg tcgaatgcgg ggcgaagcag ctggtgatta atgggtcatc
     2281 ggcatagcgg tcatgcacga gttcactacc cttgctgatg cactgcgacg cgttctccgc
     2341 ccaggcggtt gcgaaatcga tgacgggagt tccgataacc gcgcgcatgt gcagttcgga
     2401 agccgtggct gcgacaatct cgggaaaaaa gtattggtca ctgaaacacg ttatgccccc
     2461 tcgaagcatc tctgcgatgg cgtgccgggt accatcgcgg accatctctg cgctggccca
     2521 gcgtttttca ctcgggcaga tgccgtctgt cagccagcgt tccagcggca tatcgtcggc
     2581 gataccgcgg aacagggtca tggcggcatg ggtgtgggca ttgatcagcc ccggaatgag
     2641 cacgtggtct ggtcgctcca cgatctcgcg cgcttgatac ttttcccggg cgtccgctgt
     2701 cggcaggatg tcgatgatgc ggccttcgct tatggccacg gccgtttccg tcaatgtggc
     2761 atcggccggt accaccggaa cgcaccatcg cggcatgacc aatgtatcgc aggactgcat
     2821 cggccgaata gtacggttcg cgccgtacag cggcaatgca cgagcgcccg gaccagtggc
     2881 tgaacgctgt tgcggcacaa gccggatgcg ctgcgatgcc tgcagccaaa ggcttccttg
     2941 tggtatgatt acgcaccgga tttgggccgg atttggcaag gcaaatgcct cgggtgatcg
     3001 ccccggcgaa acaagaaact tccctgaaac cgaacccgtt ttttgtatat ccgtatcccg
     3061 tttatcggac ggggtcgctc cgactttgtg agtatttatg gctggaattg cgcaagagat
     3121 attgtctgta agtctcgaag acgagatgca gcagtcctat ctcgactatg cgatgagcgt
     3181 gatagtcggc cgggccctgc ccgatgtccg ggacgggctg aagcccgtgc atcgccgtgt
     3241 cctgcacgcc atgcgcgagc tgggcaacga ttacaacaag ccgtacaaga aatcggctcg
     3301 cgtcgtcggc gatgtcatcg gtaagtatca cccacatggc gataccgcgg tgtacgacac
     3361 tatcgtgcgc atggcgcaac cgttctcgat gcgctacatg ctggtggacg ggcagggcaa
     3421 cttcgggtcg gtggacggcg acatgccggc cgccatgcgt tacaccgagg tccggatgtc
     3481 gcgcatcgcg catgaaatcc tgtccgacct ggacaaggaa acggtcgact ttaccgagaa
     3541 ctacgacggc acggaatcgg agccttcggt tatgccgacg cggattccga acctgctcgt
     3601 caatggttct tccggtatcg ccgtcggcat ggcgacgaac ataccgccgc acaacctcac
     3661 cgagatagta aatgcggcgc tcgcactcat agacgatcct gctatcgaga cggcggctct
     3721 gatggaacac gtgccgggcc ccgacttccc caccgcgggt atcatcaacg gggcgcaagg
     3781 catctattcg gcttaccgca ccggccgggg acgggtgcac atccgggcac ggacgaacat
     3841 ggaaacgatc gacaagcgcg ggcgtgaagc gatcatcgtg accgagttgc cgtaccaggt
     3901 gaacaaggcg cggctgatcg aaaagatcgc cgacctcgtc cgggaaaagc gtatcgaagg
     3961 catcagcgag ctgcgtgacg agtccgacaa ggacggcatg cgcgtcgtta tcgaattgcg
     4021 gcgcggcgag aacagcgacg tcgtcctcaa caacctgttc aagcagacgc cactgcaaag
     4081 cgtcttcggc atcaacatgg tcgcgctgca cgaaggccag ccgaagctga tgaacctgaa
     4141 gcaggttctg gaagcgttcc tgtcgcaccg gcgggaggtt gtaacgcggc gcacgatctt
     4201 tgacctgcgt aaggcgcgtg accgggccca tgtcctcgaa gggcaatcgg tggcgctggc
     4261 gaacattgac gaggtaatcg cactgatcaa agcttcgcca tctccggtag aggcaaagca
     4321 gggcctcatg cagcgaaact ggtcgcccgg ggcggtcacc ggcatgctgg aacgggccgg
     4381 tgcaacggat acgcgccccg acgggctgga tccgggcttc ggtctcgcgg acggccagta
     4441 ccggctctcc gaagtccagg cgcaggccat cctcgacctt cgcctgcacc gcttaacggg
     4501 ccttgagcaa gacaagatat tcaaggaata ccaagacata ctgttgagaa ttaaggagct
     4561 gtcgagggta ctggaagacc cggacgagtt gctgcgagtg atccgggcgg agcttctcga
     4621 agtgcgagag tcttacggtg atgctcgtcg caccgagatc atcaaggacc ataccgacct
     4681 cagtctcgaa gacctgatcc tgccggaaga agtcgtggtg acactgtcgc acggcggcta
     4741 tgccaaggcc cagccgatag atgtctacca ggcgcagcgc cgcggtggtc gcggccgttc
     4801 ggccgcgaag gtcaaggacg aagacttcat cgacaagttg ttcgtcgcca atactcacga
     4861 caccctgttg tgcttttcga gtcgcggcaa gatgtattgg ctcaaggtat accaggtacc
     4921 gcaggccagc cgcggttctc gcggcaaacc catagtgaac ctcctgccac tggaggcggg
     4981 cgagcgcata aatgca
//
LOCUS       DASUOM010000426         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1133495,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000426 DASUOM010000000
VERSION     DASUOM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133495"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFY27_08855"
     CDS             complement(<1..620)
                     /locus_tag="VFY27_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HEX5765663.1"
                     /translation="MDLIVGFLLSILGTALDVLPVAFFMLFFYRVVLRQRLANRRDIL
                     IGLAFVVVGLAMFLQGLEKALFPVGRMMVEQLTSNTLATAEIPHWSSYYPVYAFAFCI
                     AFGAAIAEPALLAVAHRVNEISGGAIHSWGLRVAAALGVAFGVTLGCVRIVVGVPLHW
                     CLAAGLMIIAVQTFTSPRSILALAYDTGGVSTTAVTVPVVTALGLAL"
     gene            complement(611..1342)
                     /locus_tag="VFY27_08860"
     CDS             complement(611..1342)
                     /locus_tag="VFY27_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX5765664.1"
                     /translation="MGLLWRMTLGSIRDLTPVVVVILFFQIVVLQQPASQLLGLIEGA
                     LLVLFGLILFVYGLELALFPIGDALANALAERGSFILLLVFAFLLGFGTTFAEPALTA
                     VAAQAADAAAAGGAIASTDVAKDGYAFGLRLTVALSVGVALLLGVTRIVLGWSLPLMI
                     ICGYILVIAIATIAPPETIGIAFDAGGVTTSVITVPLVTALGIGLASSLAERNPMTDG
                     FGMIVLVLLTPMIFVMAYGTLLAWI"
     gene            complement(1508..2329)
                     /locus_tag="VFY27_08865"
     CDS             complement(1508..2329)
                     /locus_tag="VFY27_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5765665.1"
                     /translation="MGIGEVKDGGPSFDLVDIGANLAHDSFDSDRDEVIERAANCGVR
                     RLIITGSSAGSSEDAAKLAASRPGLLYATAGVHPHHASEYTPEVHDRLLALAHSDPIV
                     AVGECGLDYFRDFSPRQAQQDAFAAQLALAASTGLPLFLHQRDAHADFVSILGPMIDR
                     VAGGVAHCFTGDAAALRAYLDMGLYIGITGWICDERRGSELRELASEIPLDRLMIESD
                     APYLLPRTLQQKPRTRRNEPMYLREVLRVFAEASGVPERQIADATTRNAERLFAL"
     gene            complement(2329..3348)
                     /locus_tag="VFY27_08870"
     CDS             complement(2329..3348)
                     /locus_tag="VFY27_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5765666.1"
                     /translation="MLKRVYAIFRARNLEFVRDRSSLSWNIILPVALMFGLSFIFSGG
                     TPPAYTVGVLQGDEEVDPATHPFLETRHIQFVPVQDPEDAMSKVARHQLDMLVSFDGA
                     TRYWVNPESSKGYFVEFALLQTAGNEEIAKEQIRGEAVRYIDWVLPGILGMNMMFSCL
                     FGVGYVVVRYRKNGFLKRLRATPLRAAEFVVAQVASRLMLIMLITGFVYAGTSWFLET
                     RMEGSYAALILVTVIGAISLIAMGLVVAARVTSEELAGGLLNLVTWPMMMLSGVWFSL
                     EGSAAWVQQSAKIFPLTHVLDAARGIMIDGASLADVLPQVASLCLMSLFFLTVAAAIF
                     RWGPR"
     gene            complement(3359..>4016)
                     /locus_tag="VFY27_08875"
     CDS             complement(3359..>4016)
                     /locus_tag="VFY27_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765667.1"
                     /translation="GIQFQNTALQDFLSVRETLELFHSLYDRKADVEHIIDECSLRDL
                     LDRDNRKLSGGQRQRLLLAVALVNRPRLVFLDEPTTGLDPQARRNFWELVKRIRSEGT
                     TLVLTTHYMDEAHVLCDEIAIMDTGRIIAHGNPDILLQRQYGDVVIELPLDDVSGALD
                     SIEHRVLQTRGIIEISTKDVNATLQELASRVTGLNRLKIRQPNLEDLFLDLTGHSLRT
                     "
BASE COUNT          856 a         1224 c         1214 g          722 t
ORIGIN      
        1 agagcgagcc cgagggcggt caccacagga acggtgacgg cggtcgtgct gacgccgccg
       61 gtatcgtaag cgagggcgag tatggagcga ggtgaggtga aggtttgaac ggcaatgatc
      121 ataaggcctg cggcgaggca ccagtgcagt ggaacgccaa ccacaatccg aacacagccg
      181 agcgtgacgc caaaagcgac gccgagggcg gccgccacac gcaggcccca ggagtggatg
      241 gcgccgcccg agatctcgtt gactctgtgc gccacggcaa gtagcgccgg ttctgcgata
      301 gcggcgccga aagcaatgca aaaagcaaag gcataaaccg gatagtagct cgaccagtgg
      361 ggaatttctg ccgtggcgag ggtgttcgag gtcagttgct ccaccatcat gcggccgact
      421 ggaaacagtg ccttttcgag accttgcagg aacatcgcga gacccaccac cacgaatgcc
      481 agcccgatga ggatatccct gcggttggca agcctctgtc gcaggacaac gcggtagaaa
      541 aacagcatga agaaggcgac cggcagtacg tccagcgcag tgcccaggat gctgagaagg
      601 aatcccacta tcagatccat gcgagcaggg ttccgtacgc catcacgaag atcatcggtg
      661 tcagcagcac cagcacgatc atgccaaagc catcggtcat cggattcctc tccgccagcg
      721 aggaggcaag cccgatgccg agcgcggtta caagcggcac ggtgatgacc gacgtggtga
      781 cgccgcctgc atcgaacgcg atgccgatcg tctcaggcgg agcgatcgtg gcgatagcga
      841 tgacgaggat gtatccgcag atgatcatca ggggcaggga ccaacctaag acgatgcggg
      901 tcacgccgag caagagtgcg acaccaacag acagcgcgac cgtaaggcgc aggccaaagg
      961 cataaccgtc cttcgcaacg tcggtgctgg caatggcccc gcccgcggct gcggcatccg
     1021 cagcctgggc cgcaaccgcg gtcagggccg gttctgcgaa agtagtgccg aacccgagga
     1081 gaaaggcaaa cacgaggagc aggatgaaac tgcctcgttc ggccagtgca ttagccagtg
     1141 cgtctccgat cgggaacagg gcgagctcga gcccgtagac gaacaggatg agcccgaaga
     1201 ggaccagtaa ggcgccttcg atgagtccca acaactggct ggctggttgc tgtaagacga
     1261 cgatctggaa aaaaagtatt acaacgacga cgggggtcaa atcccggatg ctgccaagcg
     1321 tcattcgcca taggaggccc atgacgttct tattgaagtt ctgtcatacg ggcctgcaat
     1381 tcatttcccc atcgatgctc aacctccagt cgtagctctg aatactccgc gcccttggat
     1441 ccgttcatgg cagcatgcct gtcggcagta tatatcaccg cccgcgtccc tcgcagccga
     1501 gacgtcgtca caacgcaaaa aggcgttcag catttctcgt ggtagcgtcc gcgatctgtc
     1561 gctcgggaac accactcgct tcggcgaata cacgaaggac ctcgcgcagg tacatcggtt
     1621 cgttgcggcg cgtcctgggt ttctgctgca gcgtccgcgg taacaggtag ggtgcgtcgg
     1681 attcgatcat aagccggtcg agagggatct cgctcgcaag ctcgcgaagc tctgagcctc
     1741 ttcgctcatc gcaaatccac ccggtgattc cgatgtatag gcccatgtcg aggtaagcac
     1801 gaagcgccgc cgcatcgccc gtaaagcaat gagcgacccc gcccgctacg cggtcgatca
     1861 tcggcccgag aatcgagacg aaatcggcgt gtgcatcccg ctggtgcagg aatagcggca
     1921 agcccgtgct tgccgccagc gcaagctgcg ccgcgaaagc gtcctgctgg gcttgcctcg
     1981 gagagaaatc gcggaagtag tccaggccgc attccccaac cgcgacgatc gggtcgctat
     2041 gcgccaacgc cagcagccgg tcgtgtactt ccggtgtgta ctcgctggca tgatgcggat
     2101 gaacgcccgc cgttgcatac aacaggccgg gccggctcgc ggcaagtttc gccgcgtctt
     2161 cactgctccc ggcactgctg ccggtgatga tgagacgccg tacaccgcag ttcgccgcac
     2221 gttcgatcac ctcgtcgcgg tcgctgtcga agctgtcgtg tgccaggttt gcaccgatgt
     2281 cgaccaggtc gaagctcggg cctccgtcct tcacttcgcc aattcccact aacgcggacc
     2341 ccagcggaag atcgccgcgg cgacggtcag gaaaaacaag ctcatcaggc agagactggc
     2401 gacctgcggc aggacgtccg ccagggatgc tccgtcgatc atgatgccgc gagccgcatc
     2461 cagtacgtgc gtcaagggaa agatctttgc ggattgctga acccatgcag cacttccttc
     2521 gagggagaac cagactcccg acagcatcat catcggccag gttaccaggt tcagcaggcc
     2581 gccggcgagc tcctcactgg taacccgggc tgcgaccacc aggcccatcg caatcaggct
     2641 gatcgcgccg atgacggtga ccaggatgag ggctgcgtag ctgccctcca tgcgggtctc
     2701 caggaaccag ctggtacccg cgtaaacgaa gcctgtgatg agcataatta gcatgagtcg
     2761 tgatgccacc tgtgccacga caaactcggc agccctcaac ggcgtcgccc gcaggcgttt
     2821 caggaatccg ttcttgcggt agcgaaccac cacgtaaccg acgccgaaca ggcagctgaa
     2881 catcatgttc atgccgagta ttcctggcag cacccagtcg atgtagcgta ccgcctcgcc
     2941 gcggatctgc tctttcgcta tctcttcgtt gccggccgtt tgcaacaatg cgaactcgac
     3001 gaaatagccc ttcgacgatt ccgggtttac ccagtagcgg gtcgctccgt caaaactgac
     3061 cagcatgtcc agctggtgcc tcgcaacttt cgacattgca tcctcagggt cctgtaccgg
     3121 aacgaactgg atgtgccgcg tctcgaggaa aggatgcgtt gccgggtcca cttcttcatc
     3181 cccctgcaac acacctaccg tgtaggcggg tggcgttccg ccactgaaga tgaaggagag
     3241 gccgaacatc aacgcgaccg gaaggatgat gttccagctc agcgagctgc ggtcgcgcac
     3301 gaactcgagg ttgcgcgcgc ggaagatggc gtagacccgt ttcagcatgc gcggagcact
     3361 atgtccgcaa cgaatggccg gtcaggtcga ggaacaggtc ctccaggttc ggctgccgta
     3421 tcttcagcct gttgaggccg gtgacgcgcg aggccagctc ctgcagcgtg gcatttacgt
     3481 ccttcgtcga aatttcgatg atccccctgg tctgcagcac gcgatgctcg atgctgtcga
     3541 gagcgccgct cacatcgtcg agcggcaact cgatcaccac gtcgccatac tggcgctgca
     3601 gcaggatgtc cgggtttccg tgggcaatga tacggcccgt atccatgatt gcgatttcat
     3661 cgcacagcac gtgagcttcg tccatgtagt gcgtggtgag gaccagtgtc gttccttccg
     3721 acctgattcg cttcaccagt tcccagaagt tccgccttgc ctgtggatcg agccccgtcg
     3781 tcggttcatc gaggaagacg agcctggggc gattcacgag cgctacggcg agcagcaggc
     3841 gctggcgctg tccgcccgag agcttgcgat tgtcacggtc cagcaggtcc cgaagcgaac
     3901 attcgtcgat gatatgctcc acatcagcct tgcggtcgta aaggctgtgg aaaagttcca
     3961 gtgtctcgcg aacgctgagg aaatcctgca acgccgtgtt ctggaactgt atgccg
//
LOCUS       DASUOM010000427         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_288989,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000427 DASUOM010000000
VERSION     DASUOM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288989"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFY27_08880"
     CDS             complement(<1..769)
                     /locus_tag="VFY27_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5765668.1"
                     /translation="MQARISNKPVATSTQRDGRDAIRTGLGADAIAQALIDNLHYLQA
                     KLPQHATRNDWYMALAYTVRDRMLDRFIKTVETISAPESSAKIVAYLSAEFLTGPHLG
                     NSLINLGIWQAANEAVSRFGRNLFELLEQEEEPGLGNGGLGRLAACYMDSLATLSVPA
                     IGYGIRYEFGIFDQAIRDGWQVELTDKWLRFGNPWEIVSSEVNFNVSFGGRTEHYYDE
                     QGRYRMRWHPEKVVKGVAYDTPVPGYRGPTTDLLRLWK"
     gene            complement(1138..>2540)
                     /locus_tag="VFY27_08885"
     CDS             complement(1138..>2540)
                     /locus_tag="VFY27_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765669.1"
                     /translation="IVTLLTDVARLDTVYRDVHLRRAGQLFSSVLDEASYRAIGSAEK
                     EIEDLTRRSRSAVLQRNWAEAAELAVQIDSKRQRVTQTGNLATLAKEIYDPETIVFDP
                     FSPGKHLGPNAQAAQPGQRAQVMDALATLTKLDSAAGAFYEKRRSYFSGLELQTGAVS
                     KASAQRDRAQTEKQAMEAAERGDGAALQRLAKELRDWKESGTASVTDNAPVMLSRFEC
                     SVDLSAPFPPAVVERARELGLVEVRTAPVAELAKVREVIYTHVDQPIPSNPDMEREGV
                     LRARALVENELPTELDTEEPRVLVGQFIQQIFVNSGGARYVPPLSAETVLLEDFTENE
                     SADAPSKLLAAMGLPKRSGRSRAEIESALMRSGSEILQDALGLDPVEFRLICIPYELY
                     ARVGRERGFGKWAHWTHFDGYQVMGGNRLRALVGGDGRFGGLFDLVSISQSDSRDGVY
                     ARFAVVRRARMTMRWR"
BASE COUNT          495 a          788 c          723 g          534 t
ORIGIN      
        1 ctttccacaa gcgcagcagg tcggtagtcg gtccgcggta accgggcacg ggggtatcat
       61 aggcaacacc cttcacgacc ttctccgggt gccagcgcat ccggtaacgg ccctgctcat
      121 cgtagtagtg ctccgtgcgg ccgccgaagc tcacgttgaa attcacctcc gaactgacaa
      181 tctcccaggg attgccgaag cgcagccact tgtcggtaag ctcgacctgc cagccgtcgc
      241 gaatcgcttg atcaaaaatg ccgaactcgt agcggatgcc gtagccgatg gcaggaacgc
      301 tgagggtcgc cagcgaatcc atgtagcagg cggccaaccg tccgaggccg ccattgccga
      361 ggcccggctc ctcttcctgt tcgagcagct caaagagatt tcgcccgaat ctggataccg
      421 cttcgttcgc cgcctgccag atgccgagat tgatgaggct gttacccaga tgcggcccgg
      481 tcaagaattc agccgagaga taagcgacga tcttggcgga gctttccggg gcactgatcg
      541 tttctacagt cttgatgaag cggtcaagca tgcgatcgcg cactgtatag gcaagggcca
      601 tgtaccagtc gttgcgcgtg gcatgctgcg gcagcttggc ttgaagatag tgcaggttat
      661 cgatgagggc ttgtgcgatc gcatcggctc cgagcccggt gcgaatcgca tcacggccgt
      721 cgcgctgcgt tgaagttgcg accggtttat tgctaatcct ggcttgcatg ggtcctccga
      781 atgtcgagta ccgagtgtcg ggtgtcagat ccagatcagt gctacatcac agcctgaaac
      841 ctgacacctg gccatccctc ggttcgacgc ttatcctcga ccgttatcta accagacggc
      901 tccaaaacgg cgctattgga ccttggtcca acagcgctca tgtcgattgt tggtgggcta
      961 atgtttcttt ttagttttcg cccggcggac gagagcgagc acgtgtcagg cgtcaggaac
     1021 atcctgaaac ctgaacaccg aacctgaaac cttaacactg atggattgcg aggttttcac
     1081 gtgtcgggtg tcaggtgtca gtcccctcga cactcgaccc tcatccctcg ctcctcgcta
     1141 tcgccagcgc atggtcatgc gcgcgcgcct caccactgcg aaacgcgcat atacgccgtc
     1201 gcgcgaatcg gactggctga tgcttactag atcgaagagc ccgccgaagc gtccatctcc
     1261 accgactaga gcgcgcagcc gattgccacc catcacctga tagccgtcga aatgagtcca
     1321 atgcgcccat tttccgaaac cgcgttcgcg accgacgcgg gcgtataatt cgtagggaat
     1381 gcaaataagg cgaaactcga ccgggtccag accgagggca tcttgcaaga tctccgaccc
     1441 ggaacgcatg agcgcggact cgatctccgc acgggagcgg ccgctgcgct tgggcaatcc
     1501 cattgcggcg agcagttttg aaggggcgtc ggcagattca ttctccgtga aatcttccaa
     1561 cagcaccgtt tcggcggaca gcgggggaac gtagcgcgct ccgccggaat taacaaagat
     1621 ctgctgaatg aactgtccca ccagcactcg aggctcctcg gtgtcgagct cagtcggaag
     1681 ttcgttctcc accagtgccc gagcgcgcag aacgccttca cgttccatat ccgggttcga
     1741 cggtatcggt tggtcgacgt gcgtgtaaat aacttctcga accttggcta attcggccac
     1801 aggggccgta cgcacttcca ctagtcccaa ctctcgagcc cgctcaacga ctgcgggggg
     1861 aaacggtgca gagagatcga cgctacattc aaaacggctg agcatcaccg gagcgttatc
     1921 cgtgactgac gcggttccgc tctctttcca gtcccgcaac tcttttgcca gacgctgcag
     1981 cgctgcgccg tctccccgtt ccgcggcttc catcgcctgt ttctctgtct gcgcccggtc
     2041 gcgctgcgca ctcgctttag aaactgctcc ggtctgtagc tcaagcccgg aaaaataact
     2101 gcggcgcttt tcgtaaaagg cgccggcagc ggaatccagt tttgtcagcg tggcgagcgc
     2161 atccatcacc tgcgctcgct gccccggttg cgcggcttgc gcattgggtc ctaagtgttt
     2221 gcccggcgag aacgggtcga aaacgatcgt ctcgggatcg tagatctcct tcgcgagcgt
     2281 cgcgagattg ccggtttggg tgacccgttg gcgcttgctg tcgatctgca ccgccaactc
     2341 ggccgcctcc gcccagttgc gctggagcac cgcgctacgg ctgcggcgcg tcaaatcctc
     2401 gatttccttt tccgcggagc cgatcgctcg gtaggacgct tcgtcgagca ccgagctgaa
     2461 taactgcccg gcacgtcgca aatggacgtc gcggtagact gtgtccagcc tggccacatc
     2521 cgtcaacaga gtaacgattt
//
LOCUS       DASUOM010000428         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_537699,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000428 DASUOM010000000
VERSION     DASUOM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537699"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFY27_08890"
     CDS             complement(<1..571)
                     /locus_tag="VFY27_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5765670.1"
                     /translation="MARDLHWLLEGIVDAPAVPVSGITTDSRSVSPGDVFLAHQGASS
                     HGLDFVAQAVRAGAVAVVWDSATGAGRESQAGVTTVAVPGLSYRLGQIANRWFNAPSE
                     RMSVSGVTGTNGKTTVAFLIAQSMLLLDRSCAYMGTLGAGIGTLDRDIGLTTPGCIDL
                     HRKLSGFLAEGAAIAAMEVSSHALHQKRID"
     gene            complement(611..2413)
                     /locus_tag="VFY27_08895"
     CDS             complement(611..2413)
                     /locus_tag="VFY27_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5765671.1"
                     /translation="MKRKAVDRQQNAGRFVVRSILVLSFFVLVGSSLLARAVHLQVFN
                     TEFLNQQADSRHLRTEKITAHRGAITDRNGEPLAISTPVDSVWANPKELAAAVDSVPR
                     LASALGVDSPQLMRRITRSMDKDFLYLRRHLSPDHAARVMALKLPGVNVQREYRRFYP
                     AGEVTGHLVGFTSVDDDGQEGLELAFNHWLAGESGAKRVLKDRLGRSIENVESIRPPH
                     PGKDLKASIDLRVQYLAYRSLKAAIQRHRADAGSIVVLDVDSGEVLAVVNQPTYNPND
                     RAQYTAERYRNRAITDIFEPGSTVKPLVIAAALESGQYEADSMVDTAPGFLMVGPKRI
                     EDRRNLGRVSLTTVLARSSNVGATKIAMSLEPDLLWRTMTKFGLGSLTASGFPGESAG
                     LLTHYSHWQEISQATLAYGYGLSVTPLQLAHAYAGLGNGGYMRPVSLVANDQSIELKE
                     GTQVIDEDIAQAVREMLEEVVRPGSTGAKAGVTGYRVAGKTGTAWKFAAGGYSKDKYL
                     SIFAGLAPASDPKLAAVVIIDEPHGDLYYGSDVAAPVFAEVMTEALRLLAVPPDALPA
                     RDPGSVMQAMSKSAITKSTMSQSTISKSAMSTSN"
     gene            complement(2410..2700)
                     /gene="ftsL"
                     /locus_tag="VFY27_08900"
     CDS             complement(2410..2700)
                     /gene="ftsL"
                     /locus_tag="VFY27_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5765672.1"
                     /translation="MEVANAPRQPTLLALVFAVICVISAMALVYTRHESRKLFIELEQ
                     LTTERDELNIEWGQLQIEQSTWATHARIERLATEELSLTRPVATEIFVIERK"
     gene            complement(2700..3644)
                     /gene="rsmH"
                     /locus_tag="VFY27_08905"
     CDS             complement(2700..3644)
                     /gene="rsmH"
                     /locus_tag="VFY27_08905"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5765673.1"
                     /translation="MSAAGTNENRHTPVLLRAVIAALNIRPDGCYVDGTFGRGGHSSE
                     ILKRLSSGGRLIAIDRDPAAVAAVSPALKRDPRFEIVKNEIADIRQVAAERNLLGKVD
                     GFLLDLGVSSPQLDDPERGFSFRGDGLLDMRMDPDAGISAAAWLATVGEKELKRVLQR
                     FGEERSAGRIAAAIVAARAKKPISRTSELAEIVATAAPARGQKRHPATKTFQAIRIYL
                     NDELKQLEQALDASVELLAPRGRLCVISFHSLEDRRVKRFMRNAAREPEAYRGLPSIP
                     ESQRPPFRLVGKVQVASDEEVQANPRARSARLRVAERV"
     gene            complement(3654..4103)
                     /gene="mraZ"
                     /locus_tag="VFY27_08910"
     CDS             complement(3654..4103)
                     /gene="mraZ"
                     /locus_tag="VFY27_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5765674.1"
                     /translation="MFRGATKVTLDAKGRLAIPTRHRERLAARCEGQLVATVDRDYCL
                     LIYPFPDWEEIERKLVRLPSLNRQARRLQRLMVGYATELEIDAHGRILLPKELREFAG
                     LGRQAILIGQGNKFELWDEESWNEKRDAWLGEDDEVDLPAELESLSL"
     gene            complement(4517..4894)
                     /gene="rnpB"
                     /locus_tag="VFY27_08915"
     ncRNA           complement(4517..4894)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFY27_08915"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5124..>5352)
                     /locus_tag="VFY27_08920"
     CDS             complement(5124..>5352)
                     /locus_tag="VFY27_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765675.1"
                     /translation="IDDGRIPVKGEFVVAVEGSEGRATAPGIEVDRLLTELLAVVPGR
                     KAVEIAAAVSGESRNTLYRKMLRIANRGDSD"
BASE COUNT          933 a         1741 c         1577 g         1101 t
ORIGIN      
        1 cgtcgatgcg cttctggtgc aaggcatggg acgaaacttc catcgccgcg attgccgctc
       61 cttcggcaag gaaccccgac agcttgcggt gcaagtcgat gcaacccggc gtcgtcaggc
      121 cgatatcgcg gtccagggta ccgatgcccg cgcccagcgt tcccatgtaa gcgcaggaac
      181 ggtccagcaa cagcatggac tgggcaatga ggaacgccac tgtcgttttg ccgttcgtgc
      241 cggtaacgcc actgacgctc atgcgctccg acggcgcgtt gaaccatctg ttggcgatct
      301 ggcccagcct atagctaagg ccaggcacgg ctaccgtggt gacaccggct tggctctcgc
      361 ggccggctcc cgtcgcactg tcccacacca ccgctacggc gcctgctctc accgcctgcg
      421 cgacaaagtc gagcccgtga ctgctcgcac cctggtgagc caggaacacg tcccccgggg
      481 acaccgacct gctgtccgtg gtgatcccgc tgactggcac ggcgggcgcg tcgacgattc
      541 cctcgagcaa ccagtgcagg tcccgcgcca tcgtccctgt tttagcttgt gacattgcag
      601 ccaggctcat ctaattgctc gtcgacattg cggatttgct tatggttgac tggctcatgg
      661 ttgatttggt tattgctgac ttactcattg cctgcatgac gctgccggga tcgcgtgcgg
      721 gcagcgcgtc gggaggaacc gcaagcagcc gcaaagcttc cgtcatgact tcggcaaaca
      781 ccggcgcggc gacgtcgctg ccgtagtaaa gatcgccatg cggctcgtcg atgatcacga
      841 cagcagcgag tttcgggtcg ctggccggcg caaggcccgc gaaaatcgag aggtacttgt
      901 ccttcgagta tccgccggcg gcgaacttcc acgcggtacc cgtcttcccg gccacgcggt
      961 agccggtgac gccggccttg gcgccggtac tgccgggacg cacgacctct tccagcatct
     1021 cgcgcacggc ttgcgcgata tcctcatcga tgacttgcgt cccttccttc agctcaatcg
     1081 actggtcgtt cgcgaccagg gacacggggc gcatgtagcc gccattaccg agcccggcat
     1141 aagcatgcgc cagctgtaaa ggcgtaaccg acagcccata cccatacgcg agcgtcgcct
     1201 ggctgatttc ctgccagtgg ctgtagtgcg taagcaagcc cgcggactcg cccgggaagc
     1261 cgctggcggt gagggaaccc aggccgaact tggtcatcgt tcgccacagc aggtcgggct
     1321 cgagggacat ggcgatcttc gttgcaccga cgttcgacga acgcgccagc acggtcgtca
     1381 ggctgacgcg gcccaggttc ctgcggtcct cgatacgctt cggtccgacc atcaggaacc
     1441 ctggtgcggt atcgaccatg ctgtcggcct cgtactggcc gctctcgagg gctgcagcta
     1501 tcaccagggg cttcaccgtg gatcccggct cgaagatgtc ggtgatggcg cgattgcggt
     1561 aacgctccgc cgtgtattgc gcgcgatcgt tcgggttgta tgtcggctgg ttcaccaccg
     1621 ccagcacttc gcccgaatcg acgtccagca cgacgataga tccggcgtcc gcgcggtgcc
     1681 gctgtatcgc tgccttcagt gaccggtacg ccaggtactg cacacgcagg tcgatgctcg
     1741 ccttgaggtc cttacccgga tgcggcggcc ggatgctctc gacgttttcg atcgagcgtc
     1801 cgaggcggtc cttcagcact cgcttggcgc cggattcgcc ggcaagccag tgattgaagg
     1861 cgagttcgag tccctcctga ccgtcgtcgt cgacactcgt gaagcccacc aggtggccgg
     1921 tgacctcgcc ggccggatag aagcggcgat attcgcgctg cacgttcact cccggcagct
     1981 tcaaagccat cacccgcgcc gcgtggtccg ggctcaagtg ccgtcgcagg tagaggaaat
     2041 ccttgtccat gctgcgggtg atccgccgca tcagttgtgg cgagtcgacg ccgagcgccg
     2101 atgcgagccg tggcacactg tcgacagcgg cagcgagttc cttcgggttc gcccacacgc
     2161 tgtcgacggg cgtgctgatg gcgagcggct ctccgttgcg atcggtgatg gcgccgcggt
     2221 gcgccgtgat cttttccgtg cgcaggtgac gcgaatccgc ctgctggttc aggaactccg
     2281 tgttgaacac ctgcaggtga accgcccgag ccagcagcga cgatccaacc agcacgaaaa
     2341 acgaaagcac caggatgctc ctgacgacga agcgtcccgc gttctgttgc ctgtctaccg
     2401 ctttgcgctt catttgcgtt caatcacgaa aatttctgtt gccaccggac gggtcagcga
     2461 caactcttcg gtcgccaggc gctcgatccg cgcgtgggtt gcccaggtgc tctgctcgat
     2521 ctgcaactgc ccccactcga tgttgagttc atcccgctcc gtcgtcagct gctccagttc
     2581 gatgaacagc tttctcgatt cgtgccgggt gtaaacgagc gccatcgcgg agatgacgca
     2641 tatgaccgca aatacgagtg ccaggagggt tggctgcctt ggtgcgttcg cgacttccat
     2701 cagacgcgct ctgcaacccg cagccgcgcg cttctcgcgc gggggttcgc ctgcacttcc
     2761 tcatcgctcg ctacctggac cttgcccacg agccggaagg gcggccgctg ggactccggg
     2821 atggacggca ggccgcgata cgcttcgggc tcccgcgctg cgttccgcat gaaacgcttc
     2881 acgcgccgat cctcgagcga atggaagctg atgacgcaaa gccgcccgcg cggagcgagc
     2941 aattcgacgg aagcatcgag tgcctgctcc agctgcttaa gctcgtcgtt caggtagatg
     3001 cgaatcgcct ggaaagtctt ggtggcagga tgccgcttct gtccgcgcgc cggcgcagcc
     3061 gtcgcgacga tttccgccag ctcgctggtg cggctgattg gcttctttgc gcgcgcagcg
     3121 acgatggccg cggcgattct cccggccgat cgttcttccc cgaaacgctg caacaccctc
     3181 ttcaattctt tttcacccac cgtagccagc caggccgcgg cgctgatgcc tgcgtcgggg
     3241 tccattcgca tatccagcaa cccgtctccg cgaaaactga atccgcgttc cgggtcgtcc
     3301 agctgcggtg aggagactcc gaggtcgagc aggaagccat ccactttccc gaggagattt
     3361 cgctcggcag cgacttgtct gatatccgcg atttcatttt tgactatctc gaatcttgga
     3421 tcacgtttga gcgcaggact cactgctgcg actgccgccg gatcacgatc tattgcaatc
     3481 aatcgtcccc cggatgaaag ccttttcagt atttcgctgc tgtgacctcc gcgcccaaac
     3541 gttccgtcga cgtaacaacc atccggccgg atgttgagag ccgcgatcac cgcacgcaga
     3601 agaaccggcg tatggcggtt ttcgttcgtc cctgcggcgc tcactccacc gggctacaga
     3661 gatagcgatt cgagctcagc gggcagatcg acttcgtcgt cttccccgag ccatgcatcg
     3721 cgtttctcgt tccagctttc ttcgtcccaa agctcgaatt tgttgccctg accgatcaga
     3781 attgcctgac gtccgagacc tgcaaattcg cgtaattctt tcggcaagag gattcggcca
     3841 tgagcgtcga tttcgagttc tgtcgcgtag ccgaccatca agcgctgcag ccgccgcgcc
     3901 tgccggttga gactcggcag tcgcaccagc tttcgttcga tttcttccca gtccggaaac
     3961 ggataaatga gcaggcagta gtcgcggtcc acggtcgcca ccagctggcc ttcgcagcgc
     4021 gcggcaagcc gctcgcgatg gcgcgtcgga atcgccaggc ggcctttggc atccaatgtg
     4081 actttggtag cccctcgaaa cacggtcttg ttgatgggaa atcaggcggc gggatgcccc
     4141 cttaataccc atcccctccc actttttacc acttttcagc actatattcg gggcatccgg
     4201 cagcgtcaac gtgccgcgga aataattctt gttgtacagg tacttagagg tcattttgtg
     4261 accaaatcag ggccaattat ggcaatcatt tgcaaccagt aatcaagtac ttaacccagt
     4321 ttcctagaca gtcgctgaag gcggggtcgc caaaccgcga cgaggctcga tccatgcccc
     4381 tggtgaggtg tttccgggcc ggattgcgca gccggtcgcg acgcagaatt gaaaagacct
     4441 gcaatgcgtt gaaaatacag aaattatcga tccagaagca ctgccggagg ttttgcctgc
     4501 gtggatggat cgagaaagag agagccggcc ataagccggg ttctgtcagg ggcaatcatt
     4561 catctgggac agccgtcacc gactgcctct agcaacctac ccgggagtcc gctcggaacc
     4621 ggcggtgcgg aagatccgca tactccccta tttggtcttg ctccaggcgg ggtttaccgt
     4681 gccgcggtat gttgccaccc gcgcggtgcg ctcttaccgc accttttcac ccttgccggc
     4741 gcgctccagg gagcgcgctt gggcggtata ttttctgtgg cactttccat ggactcgcgt
     4801 cctccaggcg ttacctggcg cctcgtccga gggagcccgg actttcctcc ggcgcgcgcc
     4861 ttacggcgac accggcgatt gtctggccga ctctcacgtt ctcgcggccc gcatgataca
     4921 ggattccggc ggatgcggag gtcgcccgca atgcggactc ctacagcggc ttcgtccgga
     4981 cttcccgtga cttcggaatc ttgcgcttca ggagcccgta tcaggcgacc ctggcgccgt
     5041 cgcatggatg ccagctcgca cgcaatgcgg gttccaattg cccggaagtc cccatgcgct
     5101 gacgatctgg caccgctgaa ccctcaatcg ctgtcgccac ggttcgcgat gcgcagcatc
     5161 ttccgataca gagtgttcct gctttcaccg ctaacggcgg cggcgatttc aacggccttc
     5221 ctgcccggta cgaccgcaag cagttccgtg agcaggcgat ccacttcgat gccgggggct
     5281 gtcgccctgc cctcagagcc ctcgaccgca accacgaatt cgcccttcac gggaatacgc
     5341 ccgtcgtcga tc
//
LOCUS       DASUOM010000429         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_481698,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000429 DASUOM010000000
VERSION     DASUOM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481698"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..603
                     /gene="rsxA"
                     /locus_tag="VFY27_08925"
     CDS             22..603
                     /gene="rsxA"
                     /locus_tag="VFY27_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HEX5765676.1"
                     /translation="MTDLLLILVATVLVNNFLLVRFLGLCPFLGVSRNLEAAFGMSLA
                     TAFVLTLSSAVSYLLHEYLLVPLDLAYLRTLSFILVIAASVQFTEIMVRRLSPLLHEV
                     LGIYIPLIATNCAVLGVALLNVQEAKGFAQSVFFGFGASMGFALVLVMFAAVREKLIS
                     ADIPLPFRGTAIALVTAGIMSLAFMGFAGMARP"
     gene            640..1140
                     /gene="rsxB"
                     /locus_tag="VFY27_08930"
     CDS             640..1140
                     /gene="rsxB"
                     /locus_tag="VFY27_08930"
                     /inference="COORDINATES: protein motif:HMM:NF003475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEX5765677.1"
                     /translation="MWISIATAVSTITGIALASGLLLGYVSRRLPGNSDGLVERVNEL
                     LPQTQCAQCGYPGCRPYAAAIVDGGAAINLCPPGGDDTVRRLAAMLDREALPVTGATS
                     AASVHGVAIIDESLCIGCAWCRDACPVDAITGARNFMHTVIASECTGCELCVARCPVN
                     CISMVS"
     gene            1140..2687
                     /gene="rsxC"
                     /locus_tag="VFY27_08935"
     CDS             1140..2687
                     /gene="rsxC"
                     /locus_tag="VFY27_08935"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HEX5765678.1"
                     /translation="MRTAPTYDLGRLHGGLRLPGEKARSTSLPIQVMPVPDQLVLPLA
                     QHSGNAAQPVIGIGEKVLKGQLIADSDGVLSAPVHASSSGKVIAIEPWPVSRRYGDPA
                     PCIIIECDGRDEAVAVAEPVVPYESLPPESLLLKILQGGIVGLGGAVFPTAQKLMQAS
                     TLPVHTLLLNGVECEPYISCDDMLMREHAADVLKGAQVLMYALGIGKCYVAIESDKPE
                     AIHRIEAALAELNDARIVLKLVPTIYPSGGEDQLVQLIANTEVPSGGLPSDVGCLVQN
                     VGTAAAVHHWICQGEPLIARVTTVTGDGVKTPMNVLARLGTTVAEVVAFAGGYTDRAK
                     QLIIGGQMMGKSVTTDDVPVVKATNCILVQSELTPPLDHRPCIRCGECAVVCPVVLQP
                     QELYWFANADDEVKLREHGLVDCIECGCCDLVCPSHIPLMASFRQAKARIRELEDEKA
                     RAERARRRFEARTMRREIAEQKREDELAEQKRAAVTAGPREIQDMVKRVKEKKRPLER
                     TRKGPED"
     gene            2766..>3137
                     /locus_tag="VFY27_08940"
     CDS             2766..>3137
                     /locus_tag="VFY27_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX5765679.1"
                     /translation="MEFERREAPYVRPPTSVGVFMLQVLLALAPAALAYVWFFGFGFI
                     LNLLVAALFCAAGEAFMTHLRGRPPEVALSDFTAMVTAALIAFSLPALCPWWVTATAA
                     LFAIVVAKHLYGGMGYNIFNPA"
BASE COUNT          536 a          988 c          993 g          620 t
ORIGIN      
        1 ccttgcgatg cgggcgatgc aatgactgac ctcctcctca tccttgttgc gactgtactc
       61 gtcaacaatt tcctgttggt ccggttcctc ggcctgtgcc cgttcctcgg agtgtcccga
      121 aacctcgagg cagcattcgg catgtcgctc gctacggcgt tcgtgctgac actgtcgtcg
      181 gcggtgagtt acctgctgca cgaatacctg ctcgtgccac ttgacctcgc ctatctccgt
      241 accctgagct tcatcctcgt gatcgcggca agcgtgcaat tcaccgagat catggtgcgg
      301 cgactgagcc cgctgctgca cgaggtgctg ggcatctaca ttccccttat cgcgaccaat
      361 tgcgcggtgc tgggtgtcgc gctcctgaac gtccaggaag cgaaaggctt tgcgcaatcg
      421 gtgtttttcg gcttcggcgc gtcgatgggg ttcgcgctgg tcctggtgat gttcgccgct
      481 gtgcgcgaaa agctgatctc agccgacatc cccctgccgt ttcgcggcac cgcgatagcg
      541 ctggtcactg ccggtatcat gtccctggca ttcatgggat ttgcggggat ggcgcggcca
      601 tgaattccat cgcttccaat ttcttcctga cccactgaca tgtggatcag cattgccacc
      661 gccgtttcca ccattaccgg catcgcactc gcgtcggggc tcttgctcgg gtacgtgtcg
      721 cgtcggctgc ccggcaacag cgatggcctt gtcgagcggg taaacgaact gctgccccag
      781 acgcaatgcg cgcagtgcgg ttatcccggc tgccgcccgt acgccgcggc gatcgtcgat
      841 ggcggtgccg ccatcaacct gtgtccaccc gggggcgacg ataccgtgcg tcgtctcgct
      901 gccatgctcg accgggaagc gttaccggtc accggggcca cctcggcagc gtctgtccac
      961 ggtgtcgcga tcatcgacga atcgctttgc atcggctgcg catggtgtcg cgacgcctgt
     1021 ccggtcgatg ccataaccgg cgcccggaac ttcatgcata cggtgatcgc cagcgagtgc
     1081 accggatgcg agctgtgtgt tgcgcgttgc cccgtcaact gcatcagcat ggtgtcctga
     1141 tgaggacggc gcccacatac gacctcggcc ggcttcacgg cggactgaga ctgcctggcg
     1201 agaaagcgag atcgacgtct ttgcccatcc aggtcatgcc ggtacccgac cagctcgtcc
     1261 tgcccctggc gcagcattcg ggcaatgccg cccaaccggt gattggcatc ggcgaaaagg
     1321 tattgaaagg ccagctgatc gccgattccg acggcgtgct gagcgcgccg gtgcatgctt
     1381 catcgtccgg gaaggtgata gccatagagc cgtggcctgt gtcgcgccgg tacggcgacc
     1441 cggcgccctg catcatcatc gaatgcgacg ggcgcgacga ggctgtcgcg gttgcggagc
     1501 cggttgttcc gtatgagtcg ctgccgcctg aatcgctcct gctgaagatc ctgcagggag
     1561 gaatcgtcgg tctgggcggt gcggtatttc ccaccgcaca aaagctcatg caggcgagca
     1621 cgctgccggt gcacacgctg cttttgaacg gcgtggagtg cgaaccatat atcagttgcg
     1681 acgatatgct gatgcgcgag catgccgcag atgtgctgaa aggggcacag gtactgatgt
     1741 acgcgctggg aatcgggaag tgctacgtcg ccatcgaaag tgacaagcct gaggcgatcc
     1801 accggatcga ggcggcactc gccgagctga acgacgcgcg aatcgtcctg aagctggtgc
     1861 cgacgatcta tccgtcgggt ggcgaggacc agctcgtcca gctgatcgcc aatacggaag
     1921 tcccgagcgg cgggctgccg tcggatgtcg gatgcctcgt gcagaacgtg ggcacggctg
     1981 cggccgttca tcactggatc tgccagggcg aacctttgat cgcgcgcgtt accaccgtga
     2041 cgggcgacgg cgtgaaaacg ccgatgaacg ttcttgcgcg gctcgggacc acggtcgccg
     2101 aagttgtcgc ctttgccgga ggctacaccg acagggcgaa acagctgatc atcggcggcc
     2161 agatgatggg caagtccgta accaccgacg acgttcccgt ggtgaaggca acgaactgca
     2221 tactcgtcca gtccgaactc acaccgcccc tggatcaccg cccgtgcatc cgctgtggag
     2281 agtgcgctgt tgtctgtccc gtggtgctgc aaccgcagga actgtactgg ttcgccaatg
     2341 cggatgacga ggtgaagctt cgcgagcacg gcctggtcga ttgcatcgaa tgcggttgct
     2401 gcgatctcgt gtgtcccagt catatcccgc tgatggccag cttccggcag gcgaaagcca
     2461 ggattcgtga actcgaagac gagaaggccc gcgccgagcg ggcgcggcgg cgattcgagg
     2521 cgaggacgat gcggcgcgaa atcgcggaac agaagcgcga ggacgagctt gcagaacaga
     2581 aaagagcggc agttacggcc ggcccgaggg aaatccagga catggtgaag cgtgtgaagg
     2641 agaagaagcg ccctctcgaa cgcaccagga aaggcccgga ggactgatga agcgcttttc
     2701 gtcccggcgc ccgcagacat gttgcacggc gtttgttgcc gcctgcttcg ataccggccg
     2761 ctaaggtgga attcgaacgc cgtgaagcgc cctacgtgcg cccgccgacg agcgtcggtg
     2821 tgttcatgct ccaggtgctg ctggcgctcg cgcccgccgc cctcgcctac gtgtggttct
     2881 tcggcttcgg cttcatcctg aacctgctcg tcgccgcgct gttttgcgcg gccggggaag
     2941 cgttcatgac ccacctgcgc ggaaggccgc cggaggtggc cctctccgac ttcaccgcca
     3001 tggtcacggc cgctctcatt gccttctcgc tacccgccct ctgcccctgg tgggttaccg
     3061 ccaccgccgc gctcttcgcc atcgtggttg cgaagcacct gtacggcggc atgggctata
     3121 acatcttcaa tcccgcc
//
LOCUS       DASUOM010000430         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1077304,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000430 DASUOM010000000
VERSION     DASUOM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077304"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="VFY27_08945"
     CDS             <1..1961
                     /locus_tag="VFY27_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX5765680.1"
                     /translation="VLTVPLLWSMLGELWPAINPMELFGHEPVAWAQLALATPVVLWA
                     GWPFFVRGWQSVVSRNLNMFTLIALGTGAAWLFSVFATILPDLLPESFREESGAPPLY
                     FEAAAVIVTLVLMGQVLELRARAKTSSAIRALLKLAPKVAHRLDERGIESDIPLEEVE
                     AGDRLRVRPGESVPVDGFVLEGASHVDESMLTGEPDPVRKESEAPVSAGTTNGSGSFV
                     MRAERVGGDTLLSQIVNMVAQAQRSRAPVQRLVDKVAAWFVPAVVIIAIIAAIAWGAI
                     GPPPVYAHALLVAVSVLIIACPCALGLATPMSIMVGVGRGAQEGVLIRDAAALERLED
                     VTTIVLDKTGTLTEGKPSLQRVIAVSGSTEEEVLKLAASVESASEHPLARAIVSGAAD
                     RAVEKVDVTGFDSDPGLGVWGSVTERQVMVGNRRLMERHNVAVDELARLAEGERSQGA
                     TAIYVAIGGSPAGLLVIADAVKETTGAAIEALRRAGIRIIILTGDDSRTAQAVARKLG
                     VDEVIADVLPQDKAGIVKRLQDEGEVVAMAGDGVNDAPALAQADVGIAMGTGTDVAME
                     SAGITLVKGDLRGIARAVGLSRRTMANIRQNLVFAFGYNALGVPIAAGVLYPVFGLLL
                     SPVIASLAMSLSSVSVITNSLRLRTVDL"
     gene            complement(1992..2522)
                     /locus_tag="VFY27_08950"
     CDS             complement(1992..2522)
                     /locus_tag="VFY27_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX5765681.1"
                     /translation="MLKRCHPSSTRAHDEPDRLRRGVALSLLVGVLGTAFNGRLLAAP
                     ADKLSANKPIEVWTGPSCGCCKEWVKHLQENGFDVVSHEGGNSEARKRLGMPAKLGSC
                     HTAEIGGYAIEGHVPAREIHRLVEESPEAIGLSVPAMPRGSPGMDGPAYNNVRDPYDV
                     LLVARDGSASVFHSYR"
     gene            complement(2656..>3053)
                     /locus_tag="VFY27_08955"
     CDS             complement(2656..>3053)
                     /locus_tag="VFY27_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX5765682.1"
                     /translation="HAQEMIRKHPHVQGNMNDTLVRCINECYDCAQTCTSCADACLGE
                     EMADQLKQCIRLNLDCADVCSATGAVATRRTGSNEQLIQEMLGACALACRLCGDECER
                     HADEHDHCRICAEACRSCEQACHDARQTF"
BASE COUNT          508 a          925 c         1016 g          604 t
ORIGIN      
        1 tagtgctgac ggtgcccctg ctgtggtcga tgctcgggga gctgtggccg gcgatcaacc
       61 cgatggagct gttcggccat gagccggtgg catgggccca gttggctctc gcgacgcctg
      121 tcgtgctttg ggctggctgg ccgttcttcg tgcgcggctg gcaatccgtc gtctcgcgaa
      181 atctcaacat gttcacgctc attgcactcg gcaccggcgc cgcgtggctg ttctccgtat
      241 tcgcaacgat attgccggac ctgctgccgg agtcatttcg cgaagagtcc ggtgcgccgc
      301 cactttattt cgaagcagca gcggtcatcg tcacgctggt tctcatggga caggtactgg
      361 aactccgcgc cagggcgaag acctccagcg ccattcgcgc gttgctgaaa cttgcaccaa
      421 aggtggcgca tcgcctggac gagcgcggga tcgagagcga catacccctc gaggaggtcg
      481 aagcaggcga ccgccttcgc gttcggcccg gcgaaagtgt tcccgttgat ggcttcgtgc
      541 tggaaggagc gagtcacgta gacgagtcga tgctgacggg cgaaccggac cccgtgcgca
      601 aggaaagcga ggcacccgtg tccgcgggga ccaccaacgg tagcggcagt ttcgtgatgc
      661 gtgccgaaag ggtcggtggc gatacattgc tctcgcagat tgtgaacatg gtggcgcagg
      721 cccagcgttc gcgcgcgccg gtgcagcggc tggtagacaa ggtcgccgcg tggttcgtcc
      781 cggcggtcgt catcatcgcg atcatcgcgg cgattgcctg gggcgcgatt ggcccgccgc
      841 cggtatatgc acacgcgctg ctcgtcgcgg tatcggtgct gatcatcgcc tgtccgtgcg
      901 cacttggact cgccacaccc atgtcgatca tggttggtgt gggacgcgga gcacaggaag
      961 gcgtcctgat aagggacgcg gctgcgctgg agcggctgga agacgtcacc accattgttc
     1021 tcgacaagac cggcacgctc acggaaggca aaccgagcct gcagcgtgtc atcgcggtga
     1081 gcggctccac ggaggaggag gtgcttaagc ttgcagcgtc tgtagagtcg gccagcgagc
     1141 atcctcttgc gcgagccatt gtgagcggcg ctgcagacag ggcagtggag aaggtggatg
     1201 tcaccgggtt cgattcggat cccggccttg gtgtctgggg ctcagtcacc gaacgacagg
     1261 taatggtggg taaccgccgg ctcatggagc ggcacaacgt tgccgtcgat gagcttgcaa
     1321 ggctggccga aggcgagcgc agccagggcg cgaccgcgat ctacgttgcg ataggcggca
     1381 gcccggctgg cttactcgtc attgcggatg cagtcaagga gaccaccgga gcagcgatcg
     1441 aggcgctgcg tcgcgcaggc atccgcatca tcattctgac aggcgacgac tctcgcacgg
     1501 cccaggcggt ggcacgaaaa ctcggggtcg acgaagtcat tgcggacgtg ctgccgcagg
     1561 acaaggccgg catcgtcaaa cgcctgcagg atgagggcga agtcgttgcg atggcgggcg
     1621 atggcgtcaa cgatgctccg gcccttgcgc aggcggatgt cggtatcgcc atggggacgg
     1681 gaacggatgt ggcgatggaa tcggcaggta tcacgctcgt caagggcgac ctgcgaggaa
     1741 ttgctcgtgc cgttggcttg tcgcgccgaa cgatggctaa tatccgccag aacctggtgt
     1801 ttgcgttcgg ctacaacgcg ctcggcgtgc cgatcgccgc cggagtgctc tatcccgttt
     1861 tcgggctcct gctgtccccg gtcatcgcga gcctggcgat gagcctgtct tccgtatcgg
     1921 tcatcaccaa ctccctgcgg ttacggacgg tggacctgta gccacggggt gccggtttcg
     1981 atgccggccg gttacctgta agaatggaag acgctcgcgc tgccatccct ggctacgagc
     2041 agaacgtcgt acggatcccg cacgttgttg taggcaggtc catccatgcc gggagaccct
     2101 cgcggcatcg cgggcactga cagcccgatt gcttccgggc tttcctcgac caggcgatgg
     2161 atctcccgcg cgggcacgtg gccttcgatt gcgtaaccgc caatttcggc ggtatggcat
     2221 gacccgagtt ttgcaggcat gcctaggcgt ttccgcgcct cgctgttgcc accctcgtgg
     2281 cttacaacat cgaaaccgtt ctcctgcagg tgcttcaccc attccttgca gcatccgcag
     2341 gaagggcctg tccagacttc gattggcttg ttcgcagaca gtttgtctgc gggcgccgcg
     2401 agcaggcgtc cgttgaacgc cgtcccgagg actcccacga gtagtgacag ggcgacccct
     2461 cgccgtagcc tgtctggttc atcgtgcgca cgcgtgctcg aggggtggca tcgtttcagc
     2521 atctttttcg tctccgcggt agtaccggcc atccacccac tttatgcctt ttttgcggcg
     2581 cgatcgccac tcctcgctga agaagtcccg gcggacccgc cagcgaagag tggtaacagg
     2641 atcgcgcccg gatgatcaga acgtctgccg cgcgtcgtgg caagcctgtt cgcagctgcg
     2701 gcaggcctcg gcgcaaatgc ggcagtggtc gtgctcgtcc gcgtgacgct cgcactcatc
     2761 tccgcacagg cggcaggcca gggcgcaggc gcccagcatc tcctggatca actgttcgtt
     2821 cgaaccggtg cgccgggtgg cgaccgcgcc cgttgcactg cagacatcgg cacagtcaag
     2881 gttcaaccgg atgcattgct tgagctggtc cgccatttcc tcgccgaggc aggcgtcggc
     2941 gcaagaggtg caggtctgcg cgcagtcata gcattcgttg atgcatcgaa cgagcgtgtc
     3001 gttcatgttg ccttgtacgt gcgggtgctt gcggatcatt tcctgtgcgt gca
//
LOCUS       DASUOM010000431         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_179715,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000431 DASUOM010000000
VERSION     DASUOM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179715"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1248)
                     /locus_tag="VFY27_08960"
     CDS             complement(49..1248)
                     /locus_tag="VFY27_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HEX5765683.1"
                     /translation="MEDVWISSPGGVLAVLCAVAAFWFLVDQVTQWKLFQYVPPLLFI
                     YVTPVFLNNFDVIPSSGTPVYAGLSDFVLPAFIVLMLIKVDVPAAVRIMGKGVLVMLM
                     GTAGVVVGAVVSYFIVHRWLAPDAWKGFGALAGSWIGGTGNMAAVSEMLQTPPEQFGL
                     AVIADNVVYLVWLPILLASKNVAEQFNRWTGVADDRLRMMDSAAAVNLEDARVPEMRD
                     YLYLAAVAIGVTWLSTLMASMIFPAMQASFPGLQVILSESTWRILLLTTISLVLSTTR
                     ISTLPNATALGTAMIYVFVAGMGARASIEGLAQAPAFLLGAFIWIFIHGAFCVLGAKI
                     FRVDVHSAAIASAANIGAAASAPIVAAFHRPSLVPVSVLMALIGYALGNYVAPIAAHL
                     ARLAAGQ"
     gene            complement(1332..1964)
                     /locus_tag="VFY27_08965"
     CDS             complement(1332..1964)
                     /locus_tag="VFY27_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5765684.1"
                     /translation="MTTGLSNFGISAAIIAIACVLLLVLLFRGSASRITRIITETRGK
                     RRLRRTLRRRSRDVLEDVILPGAYGGLTRIDYAILTSGGILCIRAKHCNGTVFGSDDD
                     PQWKNVDGIYQQKFLNPAIQNAGRTRALQNIVPDVPVANLVVFTGSVQLAAPKVRNCI
                     HVRDLDSYIAKFVFGPCKIKDWDAVWLTVRSALLTDEGSRKDFRAQLSFS"
     gene            complement(2125..>2352)
                     /locus_tag="VFY27_08970"
     CDS             complement(2125..>2352)
                     /locus_tag="VFY27_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765685.1"
                     /translation="GRSIVLVTHLLNEIPPEVERVILIRAGEIVADGPKEQVLTVENL
                     EESYGTRLRISNVDGYWLAYPAASRHRDGGI"
BASE COUNT          494 a          712 c          714 g          432 t
ORIGIN      
        1 ccacagccac gtttttctca cggtgcggcc gcagtcatgg caccggcgtc actgccccgc
       61 ggctaagcgc gcaagatgcg cggcaatggg agcaacatag ttgcccagcg catagccgat
      121 cagcgccatc agtaccgaga caggtaccag gctggggcga tggaaagctg cgacgatggg
      181 cgcagatgcc gctgcgccga tgttcgcggc cgatgcgatc gccgcactat gcacgtccac
      241 cctgaaaatt ttggcgccga gtacgcagaa cgcgccgtga atgaatatcc agatgaaagc
      301 gccgaggagg aatgccggcg cctgcgccag cccttcgatc gaagctcttg cgcccatgcc
      361 cgcgacgaac acgtaaatca tcgcagtacc cagcgccgtc gcgttcggca acgtggagat
      421 gcgggtggtc gacagcacga gcgagatcgt ggtcagcagc aggatgcgcc aggtggattc
      481 gctgaggatt acctgtaagc cgggaaaact tgcctgcatg gccgggaata tcatggacgc
      541 catcagcgtc gagagccatg tcaccccgat ggcgacagcc gcgaggtaga ggtagtcccg
      601 catctccggg actcgcgcgt cctcgagatt tacggcggca gccgagtcca tcatcctgag
      661 ccggtcgtcg gcgacgcctg tccacctgtt gaattgctcg gccacatttt tcgaagccag
      721 gaggatcggc agccacacca ggtagacaac attatcggca atgacggcga gtccgaactg
      781 ttccggcggg gtctgcagca tttcgctcac cgcggccatg ttcccggttc ctccgatcca
      841 gctcccggca agcgcgccga aacccttcca cgcgtccggt gcaagccaac gatgcacgat
      901 gaagtaggag acgacggccc cgaccaccac cccggcagtg cccatcagca tcaccagaac
      961 gcctttcccc atgatgcgga ccgccgcggg cacgtcgacc ttgatcagca tcagcacgat
     1021 gaaggccggt agcacgaaat cgctgagtcc cgcgtacacc ggggtaccac tgctgggaat
     1081 aacatcgaag ttgttgagga agaccggcgt tacgtaaatg aacagcagcg gcggcacgta
     1141 ctgaaacagc ttccactgcg tcacctggtc gacgaggaac cagaatgcgg caacggcgca
     1201 gagtacggca agcactcccc cgggagagct gatccagacg tcttccatac ttgttttacc
     1261 gagcctcaga tcgagcgcat tataaagtat gcgagcagct tgagttcggc agggaacacc
     1321 cgggccgccg gtcaggaaaa gctgagttgc gcgcggaagt ccttgcgtga gccttcgtcg
     1381 gtaaggagag ctgagcggac agttagccaa accgcatccc agtccttgat cttgcagggg
     1441 ccaaaaacga acttcgcgat gtagctgtcg aggtcgcgta cgtggatgca gtttcggact
     1501 ttcggtgccg caagctgcac ggagccggtg aagacgacaa ggtttgccac gggaacgtca
     1561 ggaacgatgt tctgcagggc gcgcgtccga ccggcgttct ggatggccgg attcaggaat
     1621 ttctgctgat agatgccgtc gacattcttc cactgcggat cgtcgtcgct gccgaagaca
     1681 gtgccgttgc agtgcttcgc gcgtatgcac agtatgcccc cggatgtgag tatcgcgtag
     1741 tcgatgcgcg tcaggcctcc gtaagcgcca ggcaggatta catcttccag cacgtcgcgg
     1801 ctgcggcgcc gcaacgtgcg cctgagtctt cgtttgcccc gcgtttcggt gattatccgc
     1861 gttatcctgg acgcggagcc gcggaacagc agcactagca ggagaacgca ggctatcgcg
     1921 atgatggccg ccgatattcc gaagttggaa aggcctgttg tcacttccac tgttccagga
     1981 gcagccgcaa acgcttcgct ggcgagcgtt cgagacgccg ttgcaagcgt acccgcacat
     2041 gcggcaaggc tgatgcggaa ccggtggatc atcgcatcag gttatgtcta ttcaacatgt
     2101 tgcgcatcct agtccgtcac gtttttaaat gccaccgtca cggtggcggg atgcggcggg
     2161 gtatgccagc cagtaaccat ccacgttcga aatccgcagg cgcgtgccat aggactcctc
     2221 caggttttcc acggtcagga cctgttcctt cggaccatcg gcgacgattt cgcccgcgcg
     2281 aatcagtatg acccgctcga cttccggcgg aatctcgttc agcaggtgcg tgacgagcac
     2341 gatcgaacga cc
//
LOCUS       DASUOM010000432         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_290081,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000432 DASUOM010000000
VERSION     DASUOM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290081"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFY27_08975"
     CDS             <1..313
                     /locus_tag="VFY27_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="among the AAA+ ATPases, the YifB protease family
                     belongs to the Helix 2 insert clade; unknown function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HEX5765686.1"
                     /translation="GGKPGESSAEVRKRVCAARERQVRRSGDCNARLGNDLIEETCAL
                     TPEGWDLLEQATERFGLSARAHQRVRRVARTIADLDGEDDIMLPHLAEALSLRQFDKE
                     T"
     gene            complement(392..907)
                     /locus_tag="VFY27_08980"
     CDS             complement(392..907)
                     /locus_tag="VFY27_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5765687.1"
                     /translation="MNRDQKFFDVYSLVIGGLAAVCLGFFLIAMSVSENTQDEVLGNA
                     DEYLAVVSERLEPFGKVYLPGDDLTAAGPQAAEAVAPEPVAEVRTGPQVYNEACITCH
                     GSGIGGAPMITDAGAWEPRIAQGMDTLRQHAIEGYTGSAGYMPPKGGRMDLLDEEVYA
                     AVEFMLSQAEQ"
     gene            1245..>2893
                     /locus_tag="VFY27_08985"
     CDS             1245..>2893
                     /locus_tag="VFY27_08985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5765688.1"
                     /translation="MQYSKVLPALCAALFISACSGEPEGDADAPVERAQAAADLVLRG
                     GTIATVDASLGQQQAIAVSGYEIAAVGSNEEIEAWIGPETEVVELQGRFVMPGFIEGH
                     GHFMGLGRAKQILDLSTVRNWEEIVSMVAAAADKAQPGEWIFGRGWHQDKWDSVPEDA
                     VDGVPRNDSLSEASPDNPVLLGHASGHAAFANDAALAEAFIDNETPDPPGGTIVRTPE
                     GEATGLLRETAQRLLNDPVDEYNSRLTLEEQEQLDRERVRLAGEEALRNGVTSFHDAG
                     ASFEAIDFFKKLEEEGALPIRLYVMVRRQSNEEMDELLPHYKMVAEGNDFLTVRSIKR
                     QIDGALGAHGAWLLQPYEDLPESAGLMLEPVEEIERTAELAVKHGFQVNTHAIGDRAN
                     REVLDLYERVWQAADVSGKALRWRVEHAQHLDPADVPRFAQMGVIAAMQGIHCTSDGP
                     WIPTRLGEERAGITSYPWRDLIESGAIIGNGTDVPVEPINPIASFYASVSRMTVENER
                     FFPEHVMTRDEALTSYTINNAIAAFEEDIKGTLTPGKLADIV"
BASE COUNT          566 a          888 c          943 g          496 t
ORIGIN      
        1 gggcggcaag cccggcgagt cgagcgccga ggtacggaaa cgggtttgtg cggccaggga
       61 acgccaggtg cgccgcagcg gcgactgcaa cgcccggctt gggaacgatc tcatcgagga
      121 aacctgcgca ctgaccccgg agggatggga cttgctcgaa caggcgacgg aacggttcgg
      181 cctgtcggcg cgggcgcacc agcgcgtgcg gcgggtggcg cgcaccatcg ccgatctcga
      241 cggcgaggac gacatcatgc tgccgcacct cgcggaggct ctgagcctgc gccagttcga
      301 caaggaaact tgagcctcgt aggagcccgc catgcgggcg agtgcgctga agctggtcgc
      361 acccgcccgc acggccggct cctccctgct tctactgttc agcctgcgaa agcatgaact
      421 cgaccgccgc gtacacctct tcgtccagaa ggtccatgcg gccgcccttc ggcggcatgt
      481 agcctgccga gccggtgtag ccctcgatag cgtgctgacg cagagtgtcc atgccctggg
      541 ctatgcgggg ctcccaggct cccgcgtctg tgatcatcgg cgcaccgccg atgccgctgc
      601 cgtgacaggt aatgcaggct tcgttgtaca cctggggtcc ggtgcgtacc tccgccacgg
      661 gttcaggggc cactgcttcc gccgcctgcg gccccgccgc cgtgaggtcg tcgcccggca
      721 ggtacacctt gccgaagggc tcgagccgtt cggataccac cgccagatat tcatccgcat
      781 tcccgagaac ctcatcctgt gtattttcgg agacgctcat cgcgatcagg aaaaaaccca
      841 ggcagaccgc cgcgagtccg ccgatcacca gtgaataaac gtcgaaaaac ttctggtccc
      901 gattcaattc atcgctccgg aaaaatgggg aaatgcagcc agtgccgtcc gcgcacgggg
      961 gcggaagccg cgaattatat aacaagcgcc gggttactgc ggacgtgggc ttttttcaag
     1021 gaggcagaat cgttgtattg tgaaataccg cggagcggcc aaataacatc gcgtgtcgtg
     1081 taccatcggg gcgctggctc aaccggctgc ggaacccgat cctgtgcggc gctttgctgt
     1141 cgtttgtgct cgtgcgactg gacgatgcga gtgtcgagcg aattggctga cccgcggtgc
     1201 ggccggtgcg atgaaatcca taaccagact gccgggggcg tcacatgcaa tattcgaaag
     1261 ttttgccagc gctgtgcgcg gctctcttta tttcagcctg cagcggcgaa ccggaaggcg
     1321 atgccgacgc tcccgtggag cgcgcgcagg ccgccgcgga cctggtattg cgcggcggca
     1381 cgatcgcgac ggtggatgcg tcgctgggcc agcagcaggc cattgccgtc tctggctatg
     1441 agatcgccgc ggtcggcagc aatgaagaaa tcgaagcctg gatagggcct gaaaccgaag
     1501 tcgtcgaact ccaggggcgt ttcgtgatgc cgggattcat cgagggccac gggcacttca
     1561 tgggacttgg ccgagcgaag cagatcctgg acctttcgac cgtgcgtaac tgggaggaga
     1621 tcgtcagcat ggttgcagcg gcggccgaca aggcgcagcc gggcgaatgg atcttcggtc
     1681 gcggctggca ccaggacaag tgggactccg tgccggaaga tgccgtggac ggcgtgccgc
     1741 ggaacgactc cttgagcgaa gcatcaccgg ataacccggt cctgctggga cacgccagcg
     1801 ggcacgcagc cttcgcgaat gatgccgcgc tcgccgaagc cttcatcgac aacgaaacgc
     1861 cggatccgcc gggcggcacc atcgtccgca cgccggaggg cgaggcaacc gggctgttgc
     1921 gcgaaaccgc acagcgtttg ctgaacgatc ctgtcgacga gtacaacagc cgcctgacgc
     1981 tcgaggaaca ggaacagctg gaccgcgagc gcgtgcggct tgccggtgag gaagcgctgc
     2041 gcaatggcgt tacctcgttc cacgacgcgg gcgcatcgtt cgaggcgatc gatttcttca
     2101 agaagctcga agaggaaggc gctcttccca ttcgccttta cgtcatggtg cgccgccagt
     2161 ccaatgaaga gatggacgag cttctgccgc actacaagat ggttgcggag ggcaatgatt
     2221 tcctcaccgt gcgttccatc aagcggcaga tcgacggtgc gctcggcgct cacggtgcct
     2281 ggctgctcca accttacgag gacttgccgg agagcgcagg attgatgctc gaaccggtgg
     2341 aagaaattga acgaaccgcc gaactcgcag tgaagcacgg cttccaggtg aacacgcacg
     2401 cgatcggcga ccgcgccaat cgcgaggtgc tcgatctcta cgagcgcgtg tggcaggcgg
     2461 cggacgtgag cggcaaagct cttcgctggc gagtggagca tgcacagcac ctcgatcccg
     2521 ccgacgtgcc ccgtttcgcg cagatgggag tgatagccgc gatgcagggc atccactgca
     2581 cgtccgatgg accgtggatc ccgactagac ttggcgaaga acgtgccggg atcacatcct
     2641 acccgtggcg cgacctgatc gagtcgggag cgatcatcgg gaacggcacg gacgtaccgg
     2701 ttgaaccgat aaacccgata gcatccttct atgcgtccgt ctcacgcatg accgtggaga
     2761 acgagcgttt tttccccgag cacgtaatga cgcgcgacga agcgttaacc agctacacca
     2821 tcaacaacgc gatcgctgcg ttcgaggagg atataaaggg cacgttgact cccgggaagc
     2881 tcgctgacat cgt
//
LOCUS       DASUOM010000433         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_48195,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000433 DASUOM010000000
VERSION     DASUOM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48195"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VFY27_08990"
     CDS             complement(<1..1959)
                     /locus_tag="VFY27_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX5765689.1"
                     /translation="MSNQTMRVLNEGTRLADRYTLVRRLGSGGMSEVWLARDERAQQR
                     VALKFLSPGLVAQAEARSRFHREWQAANRLMHAHIARVFEYHDDPDGPFYSQQFVDGP
                     DISAVTGQPPDECLRPLALVADALRYAHAKGFVHRDIKASNILLDNRGAPYLIDFGVA
                     QVEGIPTAGGSPIGMSPQQRAGEGPVPADDIWALGVLMHELLSGEPPPADADVAAVLT
                     AADGSRIPEELQRIVRDALALDASERPTAEEVVERLRDAGVTPGPASIKRRAAYAGDT
                     EISAEAVTSIRPVRPEVVATGKTGQAETRGVSPKIAYGGLAVLLVVFVTVIFWLPDAI
                     QDGAEPAGDRPAASAGAADTPPGDVAPTVDEGERDVDPAAEAELKSATDEALGDLLSR
                     LQRLQYRGIEHWGGQTYLNALGVYEQGDRAYLNKDYATAGDLYREAAEMLEPFFDRID
                     GVFNETMQAARDAFAAGDHAEAVRLYDLAVAITPGHREAEEGLARARNLSSVLELVDR
                     GRTFENELDLQAAKLAFEKALELDPAWEPALSGLERVNAAIHERSFQQRMTEGLDALA
                     AGDLLTARAAFDAAKALKPDSPEPADGLLQVDQEMRLANIGRLEQEAVTLEDNEEWQA
                     AIAKYQEIGEIDSDLQFVREGLARARERA"
     gene            complement(1963..3150)
                     /locus_tag="VFY27_08995"
     CDS             complement(1963..3150)
                     /locus_tag="VFY27_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765690.1"
                     /translation="MITAEKTELEVASLDIAENMLLDVGIVNFDDGLPEDNDPDETGI
                     YPEVREAETRYLPYHLKTTLQGTGYWGAVRVIPSRYVFTDVTLSGVIEDSDGEYVTLR
                     VTAEDSLGRHWYEKEYEMQTGITSYSESRDRTQDPYQKVFNDIANDLRSHAATLPPEE
                     IKHIRQVSELRFFGDMAPDVYGEYLATNEEGVTTPVRLPANNDPMVNRLRQIRERDRL
                     VIDTLNEHYANFYYGVAIPYESWRKAAREQAVSYRQVKRSAALRALLGVVVVAGSMSI
                     DTGSSSSSSARAKQAAQYVGINRGINTIISSWQLRSSANIHRENIKELSESFGAEAAP
                     MVVQVEGESRRLTGTAEAQYESWRKLLKEIYQVETGFSEAIDVGVPARSAQQQNNAPQ
                     QNQ"
     gene            complement(3242..>3996)
                     /locus_tag="VFY27_09000"
     CDS             complement(3242..>3996)
                     /locus_tag="VFY27_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765691.1"
                     /translation="GGGASGGSMPGGMPGGDGGGGASGGDMPGGMSGGGGAAGGGAGG
                     GMDGGAGGGGGGAGGGIRGLGGQMPGTGGGSGGAGGAPGGNCEQGPPGGVGGMEGTGE
                     CAGGVGEIPGETEAERKARLEGELDKSVGGFDEVLAEEQREISTVGRDTEGFGGGAGG
                     GGGVGLGKQATGGIEGTGEQVARAESPGSGSTPSIDSMSEEELAKRTPEDIPDLVSED
                     IVAKQLREAALAEDDPVLRERLWEEYRNYNNL"
BASE COUNT          643 a         1395 c         1146 g          812 t
ORIGIN      
        1 tgcccgctcc ctcgccctcg cgaggccctc ccgcacgaac tggagatcgg aatcgatttc
       61 gccgatttcc tggtatttcg cgatcgccgc ctgccattcc tcattgtcct cgagcgtcac
      121 cgcctcctgc tccagcctgc caatgtttgc aagccgcatt tcctggtcca cctgcagcaa
      181 accgtctgcc ggctccggcg agtcgggttt cagtgccttg gcagcatcga atgcggcgcg
      241 cgccgtcagc aggtcgcccg ccgccagcgc gtccaggccc tcggtcatcc gctgctggaa
      301 agaccgctcg tgaatcgcgg cgttgacacg ttcgagtccc gagagcgccg gctcccatgc
      361 cgggtcgagt tccagtgcct tctcgaaagc aagtttcgct gcctgcaggt cgagttcgtt
      421 ctcgaaagtc cgcccccgat ccacgagttc gagcaccgat gacaggtttc gggcgcgggc
      481 gaggccttct tcggcttccc tgtggcccgg cgttatggcg acggcaagat catacaaccg
      541 gaccgcttca gcgtgatcgc ctgcagcaaa cgcgtcgcga gcggcctgca tggtctcatt
      601 gaatacccca tcgatgcgat cgaaaaaggg ctccagcatt tccgcggctt cgcgatagag
      661 gtcaccggcg gttgcgtagt ccttgttcag gtaggcgcgg tctccctgtt cgtagacgcc
      721 cagcgcattc aggtaggtct ggccgcccca atgctctatg ccacggtact gcaggcgctg
      781 caaacgggac agcagatcgc ccagggcttc atcggtggcc gatttgagtt ccgcttccgc
      841 ggcagggtcg acgtcacgct cgccctcgtc caccgtcggt gctacatcac ccggcggagt
      901 gtcagccgcc ccggcggacg ccgcaggcct gtcgcccgcg ggctcagctc catcctggat
      961 tgcgtccggt aaccagaaaa ttaccgtgac gaaaacgacg agcagcacgg cgagcccgcc
     1021 ataggcgatt ttgggtgaaa cgcccctggt ttccgcctgc cctgtctttc cggtggccac
     1081 tacctccggt ctcaccggtc gtatcgaggt cacggcttct gcggaaatct ctgtatcgcc
     1141 tgcgtacgcc gctcgccgct ttatcgaagc gggacctggt gtcacgcccg catcgcgcag
     1201 gcgctccacg acctcttcgg ccgtcggccg ctcgctggca tcaagggcaa gcgcgtcgcg
     1261 aacgatcctc tgcagttctt cggggatgcg tgacccgtcc gccgccgtaa gtaccgcggc
     1321 cacgtccgca tctgcaggcg gcggctctcc ggaaagcagt tcgtgcatca gcacgcccag
     1381 cgcccatata tcgtcggctg gaaccggacc ttcgcccgcg cgctgctgcg ggctcatccc
     1441 gatggggctg ccgcctgcag tcgggatgcc ctcgacctgc gcgacaccga aatcgatcag
     1501 gtacggcgcc ccgcggttat cgagcaaaat gttgctcgcc ttgatgtcgc ggtgaacgaa
     1561 accctttgcg tgagcgtaac gcaacgcgtc ggcaaccagc gccaacggtc gcaggcactc
     1621 atccggaggc tgcccggtca ctgcacttat gtcgggcccg tcgacgaatt gctggctgta
     1681 gaaagggccg tcgggatcgt cgtggtattc gaacacgcgt gcgatgtgcg cgtgcatgag
     1741 gcggttcgcg gcctgccact cgcgatggaa acgcgatctc gcttccgcct gggccaccaa
     1801 acccggtgaa aggaacttca acgccactcg ctgttgcgcg cgttcgtccc tggccagcca
     1861 gacctccgac atgccccctg acccgagacg gcggacgagc gtgtatctgt cggccagccg
     1921 tgtaccttcg ttcagtaccc gcatcgtctg gttggacaac tgctattgat tttgttgcgg
     1981 cgcgttattt tgctgctgcg cggacctggc gggaacgcca acgtcaatcg cttccgagaa
     2041 cccggtttcg acctggtaaa tttccttgag cagcttgcgc cagctttcgt actgcgcttc
     2101 ggcggtaccg gtaagccggc gagactcgcc ctcgacctgg accaccatag gcgctgcttc
     2161 cgcaccgaag gactccgaca gttccttgat gttttcgcga tggatgttcg cggaagagcg
     2221 aagctgccag gagctgatga tcgtgttgat tccccggttg atgccgacgt attgcgccgc
     2281 ctgcttggct cgcgcgctgg aactgctgga gctgccggtg tctatgctca tcgaacctgc
     2341 gaccacgacc acgccgagca gcgccctcaa tgccgcggag cgcttgacct gccggtagct
     2401 gaccgcctgc tccctcgcgg ccttacgcca tgactcgtaa ggaatcgcca ctccgtagta
     2461 gaagttcgcg tagtgctcgt tcagcgtgtc tatgacgagc cggtcacgtt cccggatctg
     2521 ccgcagccga ttcaccatcg ggtcgttgtt ggccggcagg cgtaccggag tcgtcacgcc
     2581 ctcctcgttg gtcgcgagat attctccata cacatcgggc gccatgtccc cgaaaaagcg
     2641 cagctctgag acctgccgaa tatgtttgat ctcttccggc ggcagtgtcg ccgcgtgact
     2701 gcgcaggtcg ttcgcaatat cgttgaatac tttctggtaa gggtcctgtg tgcgatcgcg
     2761 cgactcggag tacgaggtga taccggtctg catctcgtat tctttttcat accagtgccg
     2821 ccccagggag tcttccgctg tcacccgcaa cgtgacgtat tcgccgtcgg aatcctcgat
     2881 gactccgctc aatgtgacgt cggtgaacac gtagcgcgac gggatcacgc ggactgcgcc
     2941 ccagtacccc gttccctgca gcgtcgtttt caggtggtac ggcaagtacc tggtttcggc
     3001 ttcgcggacc tccggataaa tgccggtctc atccgggtca ttgtcctccg gaaggccatc
     3061 atcgaaattg acgatgccaa cgtccagcaa catgttttcg gcaatgtcca gcgaggcaac
     3121 ttccagctcc gttttttccg cagtaatcac ttcggatact gtgcagccgg tacagagcaa
     3181 tacccatatc agaaaaagcg gggcattttt cataacgtat ccgagcaccc ctttacctta
     3241 ctcagagatt gttgtagttc cggtactctt cccagaggcg ctcccgaaga acaggatcgt
     3301 cttccgcgag cgcagcttcg cgcagctgct tcgcaacgat gtcctcgctc acgaggtccg
     3361 gaatgtcctc cggcgtgcgt ttcgcgagct cttcttccga catgctgtcg atcgagggcg
     3421 tcgacccgct gcctggagac tctgcgcgag caacctgttc gccggttccc tcaatgccgc
     3481 cagtggcctg cttgcccagg ccgacgcctc cgccgcctcc ggcgccgccg ccgaaacctt
     3541 cggtatcacg ccctaccgtg gagatctcac gttgctcctc ggccagtacc tcgtcgaaac
     3601 cgccgaccga cttgtccagc tctccttcga gccgcgcctt gcgctcggcc tcggtttcac
     3661 ccgggatttc gccaacgccg cctgcgcact cgccggttcc ttccatgccg ccgacgccgc
     3721 cagggggccc ctgctcacag ttcccaccgg gtgccccacc ggcaccgccg gatccgcccc
     3781 cggtgccggg catctgtccg ccaaggccac gaataccgcc cccggcgccg ccgccgccgc
     3841 ctcccgcgcc gccgtccatt ccgccgcctg ctccgccacc cgcggcgccg cccccgccgg
     3901 acatgccacc cggcatatcg ccaccggatg cgccgccgcc tccgtcaccg ccaggcatgc
     3961 caccaggcat actgccaccg gatgcgccgc cgcctc
//
LOCUS       DASUOM010000434         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_290803,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000434 DASUOM010000000
VERSION     DASUOM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290803"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFY27_09005"
     CDS             complement(<1..579)
                     /locus_tag="VFY27_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765692.1"
                     /translation="MMIKIAALSAAMLGAALTGCDGGGHGAHEAPDTLPADSFMERIA
                     RHCGQAYAGRVAVNEPPTDDDPFAGRDLVMHVRDCSDDEIRIPFHVGDDRSRTWIISR
                     TYDGIRLKHDHRHQDGSEDPVTMYGGETAADGTESRQEFPVDKQSARLFEDRGMAASM
                     NNVWALEIEPDERFVYELARPGTDRLFRVEFDL"
     gene            complement(569..1123)
                     /locus_tag="VFY27_09010"
     CDS             complement(569..1123)
                     /locus_tag="VFY27_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765693.1"
                     /translation="MNTLTDTNRKQVSSGAPEAEPRSDERAGAKEKRRKKQKRPFLQG
                     TRDYLATLYGLGRTLVQEPHSFPRELKLHLLKWARATWNARGGGFYACGFVVTFLFLE
                     VKLLLTELFTSAGSADFLIGQVFQLVLRFTVDSLVNTLHALIWPAMVLSVSPVNGVVF
                     LVVGYLIFKRFLKEPLTRVLFDDD"
     gene            complement(1212..1763)
                     /locus_tag="VFY27_09015"
     CDS             complement(1212..1763)
                     /locus_tag="VFY27_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX5765694.1"
                     /translation="MNRIFSRSCVMLAGAFLLAPATSLAASAGEIDASVSEALDLFAE
                     QVDGGRNFLDKAAGYLVFPRVIKVGIGLGGETGEGALLIKGKTAGYYRTASGSIGFQL
                     GAQAKSIVIAFMTPESLEKFRNSAGWKVGVDGSVALIDIGAGKAIDTTSINDPVVGFV
                     FGAKGLMYNLTLEGSKITRIDKS"
     gene            complement(1827..2162)
                     /locus_tag="VFY27_09020"
     CDS             complement(1827..2162)
                     /locus_tag="VFY27_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765695.1"
                     /translation="MKRCGALLLLVMLGPVALAEEPVDEATGLRVAPGWELVRAHCGG
                     CHSHKLVTSQRADRQTWLDIMRWMQATQNLWQFDAATENSILDYLAANYPPRQNRRRA
                     PIPPTLMPE"
     gene            complement(2164..>2989)
                     /locus_tag="VFY27_09025"
     CDS             complement(2164..>2989)
                     /locus_tag="VFY27_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX5765696.1"
                     /translation="GGNGRSEFYPPASGNQWTTGAVGCPEWTGARLADVLDWCGVRGD
                     AVYVAYEGADLHLSGDPTKRPISRGVPLAKAREKESLLAWAMNGEPLPLMHGQPLRML
                     CAGWPASVSGKWVNRILIRDRVHDGEKMTGKSYRVPCEPVAPGAEVPDDNMCIIGSMP
                     VRSLVTFPRSGATQPLGEALALRGHAWAGDSRVRSMYTSIDFGTTWQKAKLERPANRL
                     AWQRFRQSIEFPGRGYYEVWARAEDSEGRSQPMVLPGWNPQGYLNNACHRIAVSVV"
BASE COUNT          529 a          989 c          901 g          570 t
ORIGIN      
        1 gagatcgaac tccaccctga aaaggcgatc cgtgccgggg cgggcgagtt cgtacacgaa
       61 acgttcatcc ggctcgatct ccagggccca gacgttgttc atcgaggcgg ccatgccccg
      121 gtcctcgaac agcctggccg actgcttgtc gactgggaac tcctgccgtg actccgtgcc
      181 gtcggcggcc gtttctccac cgtacatggt caccgggtcc tcgctgccat cctggtgacg
      241 atgatcgtgt ttcaggcgaa tgccatcgta cgtccggctg atgatccagg tgcggctgcg
      301 gtcatcgccg acgtggaagg gaatcctgat ctcgtcgtca ctgcagtcac gcacgtgcat
      361 cacaaggtct ctgccggcga aaggatcgtc gtccgtcggc ggctcgttca cggccacgcg
      421 gcctgcgtac gcctggccgc aatgccgcgc aatgcgctcc atgaagctgt ccgcgggaag
      481 agtgtccggc gcttcgtggg cgccgtggcc accgccgtcg catcccgtga gtgctgcccc
      541 gagcattgcc gcggacaatg ctgcaatctt aatcatcatc gaatagtacc cgggttaacg
      601 gctctttcag gaaccttttg aagatcaggt agccgacgac gagaaacacc accccgttga
      661 ctggcgacac gctcaggacc atcgcaggcc agatcagcgc atgcaaggta ttcaccagcg
      721 aatctaccgt gaagcgaaga acgagctgaa acacctgccc gatgaggaaa tcggccgacc
      781 ccgcgctggt aaagagctcg gtgaggagca gcttcacttc gaggaacagg aacgttacga
      841 cgaaaccgca ggcatagaag ccgccgccgc gcgcgttcca ggtggctcgc gcccatttca
      901 ggagatgcag tttcaactcg cggggaaagg agtgcggttc ctgtacgagc gtgcgtccga
      961 ggccgtacaa agtcgccagg taatcccgcg tgccttgcag gaaaggccgc ttctgcttct
     1021 tgcggcgttt ctctttcgcg ccggcccgct cgtcgctgcg gggttccgct tcgggtgcgc
     1081 ctgatgagac ttgtttccgg ttcgtgtcgg tcaatgtgtt caatcgccgc cggcttgcgc
     1141 ctgtgcgtca tccgatttct cgtcggcatc ggtcccgaaa ccgccggatc gtctgctccg
     1201 gtttgacagc ttcagctctt gtcgatcctg gtgatcttcg agccttcgag cgtcaggttg
     1261 tacatcagcc ccttggcgcc gaagacaaag cccacgacag ggtcattgat gctggtggtg
     1321 tcgatcgcct tgccggcccc gatgtcgatc agcgccacgg aaccgtccac gccgactttc
     1381 catcctgcgc tgttcctgaa tttctcgagc gactcgggcg tcatgaacgc gatcacgatc
     1441 gacttcgcct gcgcgcccag ctgaaaaccg atggagccgc tcgccgtgcg atagtagccg
     1501 gcggttttac cctttatcag gagtgcgccc tcgcccgttt ctccccctaa cccgatcccc
     1561 accttgatga cgcgggggaa cacgagatag cccgccgcct tgtcgagaaa gttgcggccc
     1621 ccatcgacct gctccgcgaa caggtccagc gcctcgctga cggaagcatc gatctcgccg
     1681 gcgcttgcgg ccagcgacgt cgccggcgcc agcaggaatg cgcccgcgag catcacgcat
     1741 gacctcgaga aaatcctgtt cattattaaa ttaacacctt cggtgatgac ggcaacgcgc
     1801 atcttaacgc agatttgata cagcgcttat tcaggcataa gcgtcggggg gatcggagcg
     1861 cggcggcggt tttgccgggg cgggtaattc gccgccaggt aatcgaggat ggagttttcc
     1921 gtcgcggcgt cgaactgcca caggttttgc gtggcctgca tccagcgcat gatgtcgagc
     1981 caggtctgcc ggtcggcgcg ctggctcgta accagtttgt gggagtggca tcccccgcag
     2041 tgcgcccgca ccagttccca gccgggagcg acgcgaagcc cggtcgcttc atcgaccggt
     2101 tcttccgcca gcgcgacagg gccgagcatg accagcaaca gcaatgctcc gcagcgtttc
     2161 atttcaaacg acgcttaccg cgatgcgatg acaggcgttg ttcaggtagc cttgcgggtt
     2221 ccatcccgga agcaccatcg gctgcgagcg tccttcgctg tcttcagctc gcgcccacac
     2281 ttcgtaatag ccgcgcccgg gaaattcgat cgactgcctg aagcgctgcc atgccagccg
     2341 gtttgccggc cgctcgagct tcgccttctg ccacgtagtg ccgaagtcga tcgaggtata
     2401 catgctcctg acccggctgt cgcccgccca ggcgtggccg cgcagcgcca gcgcctcgcc
     2461 gagcggctgc gtcgcgccgg aacgtggaaa cgtcacgagc gaacgcaccg gcatcgaccc
     2521 gatgatgcac atgttgtcgt ccgggacctc ggcgccgggc gcaaccggtt cgcacggcac
     2581 gcgataggac ttgcccgtca ttttctcgcc gtcgtgcacg cgatcgcgaa tgagtatccg
     2641 gttcacccac ttgccgctga ccgaagccgg ccagccggcg cacagcatgc gaaggggctg
     2701 cccgtgcatg agcggcagcg gctcgccgtt catggcccac gccagcagcg attccttctc
     2761 gcgagccttt gcaagcggta cgccgcgcga aatcggccgc ttcgtcgggt cgccggacaa
     2821 atgcaggtcc gcgccttcgt acgcgacata aacggcatcg ccgcgaaccc cgcaccagtc
     2881 cagcacgtcg gcaagccgcg cacccgtcca ctccgggcaa ccgactgcgc cggtcgtcca
     2941 ctggttgccg ctcgcagggg gataaaattc ggaacgaccg ttgccgccg
//
LOCUS       DASUOM010000435         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_652826,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000435 DASUOM010000000
VERSION     DASUOM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652826"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFY27_09030"
     CDS             complement(<1..406)
                     /locus_tag="VFY27_09030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5765697.1"
                     /translation="MKQLFLLPVLVASLVLLSGWAALLLAALGLFIVLGVVTVHVSSV
                     FIAHRPQRTDHAPKREAPDKSAGQPRVSIHVPCCKEPAKIVIGTLDALARLDWDNYEV
                     ICLDNNTPDPADWQPMEEHCRRLGSTFRFYHFD"
     gene            complement(1218..1847)
                     /locus_tag="VFY27_09035"
     CDS             complement(1218..1847)
                     /locus_tag="VFY27_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEX5765698.1"
                     /translation="MELKVAGASLLVLLALNGCASMNADECVMSDWQTIGFEDGARGY
                     TMDRLGQHRKACARHGVAPDLRAYQSGRDEGLQQFCQPSRGFNLGAGGGQYNGVCGDY
                     SEPQFLDAYRSGYHLYNLRSSVDSATYQINAKTQEIEDTREEIRSKEAALIRKDTPTE
                     ERILLLADLKDLSERTGQLESEIYLLVDERARHEEQLAAYQAVLADAGY"
     gene            complement(1949..>2763)
                     /locus_tag="VFY27_09040"
     CDS             complement(1949..>2763)
                     /locus_tag="VFY27_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765699.1"
                     /translation="ALNRFEEAYIEQDYSARPTAAEITAKSREMLATVLGDGGARDIV
                     SHPYVRLHILAVRCGRLTSSESRPLLASGMLAAAFANAVSRRTLGLFFKRVLFYDARD
                     LPPFYNAGGFPLERINLTQKNIVDAIVASGSIPMVLSGVRDIDGAPPGIYRDGGVIDY
                     HLDLPLAEPDRLTLYPHFFEQLIPGWFDKKLRWRRMNPLHTSRTILVCPSPHFISRLP
                     QGKVPDRTDFVRMAPSARMRTWRQVIAACGALADDLNEVLEKGSLAERLQPL"
BASE COUNT          506 a          864 c          864 g          529 t
ORIGIN      
        1 cgtcgaagtg atagaagcgg aacgtgctcc cgagtcggcg gcagtgttcc tccatgggct
       61 gccagtccgc cgggtccggc gtgttgttgt cgaggcagat tacctcgtag ttgtcccaat
      121 ccagccgtgc aagagcatcc agggtgccga tcacgatctt cgcgggttcc ttgcagcatg
      181 gcacgtgaat cgagacccga ggttgcccag ccgacttgtc cggagcctcc cgcttcggtg
      241 catgatcggt gcgctgtggc cggtgagcta tgaacacgga gctcacgtgg acggtaacca
      301 cgccgagaac gatgaacagg ccgagggcag ccaggagcag tgcggcccac ccggagagca
      361 ggaccaggga cgctaccagc accggcagca ggaataactg tttcacctca gggccgcgcc
      421 cggtgcccca ggtaatggtt gcacaaaaat tctccttgcc attgattttg tccgccccct
      481 ccgttgcatc tatcgtgcca atcgctgcgc gctggcaatt cctctttcac cggagcgcag
      541 gcatcaggct tatgtagtat ctaccgtcgg caaattgagg catgtagaag ttacaaccct
      601 gacggtgcga ccgttgccgc tgcaaacctg ccccggatga ccggaggacc gatgcagcgg
      661 caggagcctg tgcgcgacgc tggcggagtt gcggtatgct ttgccgctgg accggccacg
      721 ggcattagcc aagtgaaaac tgaccaagtg aaagttacca ggtgaaattg atagtcggcg
      781 ttgcggtgtt gctgatcgcc gtgttgattt tcttgcggat gcgaagtcgc gcgcccataa
      841 cgcatgagcc taaaacgcct gatcctgctg ctacggcgcg gctcaaaggc gacttccacg
      901 cggtttcaat ccgtcctgga accttcgcct gcaaggctgc gaggaatttg gagggtcagc
      961 gtttcctctc tggctcggcg ccccgtatcc ctctccccga ttgcgatgca tccgactgca
     1021 gctgccggtt cgcccaccac gcggaccggc gcggcagtga tgatcggcgc acgccctacc
     1081 gctcgcctat tggactggat gcaggagctg tcggcgagga gcagcgcctg gaagacgatc
     1141 ggcggggcag tcgctccgac ctggaatagc agcgcagtgc gctgctcacc gcgagagctc
     1201 gagcgaatcc cgtctgatca gtaaccggcg tcggcaagga ccgcctggta ggccgccagc
     1261 tgctcttcat gacgcgcgcg ttcgtcgacc agcaggtaga tttccgactc cagctgcccg
     1321 gtccgttccg acaggtcctt gagatcggcc agcaacagga tacgttcctc cgtcggcgtg
     1381 tccttgcgga tcagcgctgc ttccttgctg cggatttctt ccctggtgtc ctcgatctcc
     1441 tgcgtcttgg catttatctg gtaggtggcc gagtccacgc tcgagcgaag attgtacagg
     1501 tggtagccgg agcggtaggc gtccaggaac tggggctccg agtaatcgcc gcagacgcca
     1561 ttgtactggc cgccgccggc gccgaggttg aagccgcgag acggttggca gaactgctgc
     1621 aacccctcgt cacgtcccga ctggtaggcg cgcaagtcgg gtgcaacacc gtgcctggcg
     1681 cacgccttgc gatgctggcc aagccggtcc atcgtgtagc cccgggcacc gtcttcaaac
     1741 ccgatggttt gccagtcgct catgacgcat tcgtcagcgt tcatcgaggc gcatccattc
     1801 aacgcgagca acacgagaag gcttgctccg gccactttga gttccatgtc ccgtctcccg
     1861 tatgctcgag gcgcagccga tccgcgcaag ctcgccattc tttggggtca cgttagcagc
     1921 aggccggggc accgctccgt gatcgcgctc acaatggctg aagtcgctcc gccaggcttc
     1981 ctttctcgag gacttcgttc aggtcgtcgg ccagcgcgcc gcaggcggca attacctggc
     2041 gccaggtgcg catcctcgcc gagggcgcca tccgcacgaa atccgtccgg tccggaacct
     2101 tgccctgcgg aaggcgggag atgaaatggg gagatgggca aaccaggatc gtgcggctgg
     2161 tatgaagagg attcatgcgg cgccagcgca gcttcttgtc gaaccagccg ggaatcaact
     2221 gctcgaaaaa gtgcggatag agcgtcaggc gatcgggttc cgccagcggc aggtcgaggt
     2281 ggtaatcgat gacgccgccg tccctgtaaa tgccgggcgg agcgccgtcg atatcccgca
     2341 ccccggaaag caccatcggg atcgacccgg acgcgacgat tgcatcgacg atattcttct
     2401 gtgtgagatt gatgcgttcc agcgggaatc cccccgcgtt gtagaacggc ggcaggtccc
     2461 tggcgtcgta gaacaagacc cgcttgaaaa aaagcccgag tgtccgccgg ctcaccgcgt
     2521 ttgcgaacgc cgcggcgagc atccccgaag cgagaagcgg acgcgactcg gatgacgtga
     2581 ggcgcccgca gcgaaccgcc agtatgtgca ggcgaacgta cgggtgggac acgatatccc
     2641 gcgctccgcc atcgccgagt accgttgcca gcatctccct gctcttcgcc gtgatctcgg
     2701 cggcagtcgg cctggcgctg taatcctgct ctatgtaggc ctcctcgaac cggttcagcg
     2761 cat
//
LOCUS       DASUOM010000436         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1738237,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000436 DASUOM010000000
VERSION     DASUOM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738237"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1173)
                     /gene="rodA"
                     /locus_tag="VFY27_09045"
     CDS             complement(91..1173)
                     /gene="rodA"
                     /locus_tag="VFY27_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5765700.1"
                     /translation="MARFLRQLHIDGPLLGGLLLICCLGLVVLYSAVGENMDLWLQQC
                     IRLGIGLVVLFIVAQVPPGVMRRWTAWGYALGLALLALVLLRGDVGQGAQRWLDLGIR
                     FQPSEIMKLAVPMVAAWYLHDRQLPPRAGDLLLIALLIAVPTVMIAVQPDLGTSILIA
                     MAGMLVVVLAGLQFRVIIILGLLSVPGAWLLWRFMHEYQQNRVLMLLNPESDPLGAGY
                     NIIQSKIAIGSGGLFGKGWANGSQAQLEFLPERDTDFIFAVMGEELGLFGVLSLLGLY
                     MFVVGRGLFIAIQARETFARLLAGSISLTFFVYVFVNTAMVTGLVPVVGIPLPLVSFG
                     GTSMVTLMAGFGILMSIHTHRKLVPQ"
     gene            complement(1220..>2693)
                     /gene="mrdA"
                     /locus_tag="VFY27_09050"
     CDS             complement(1220..>2693)
                     /gene="mrdA"
                     /locus_tag="VFY27_09050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5765701.1"
                     /translation="EISRFAIQRPRFPGVDFQPRLVRHYPYGELAAHVIGYVGALSAV
                     DLERLDPAAYAGTAHTGKTGVEFYNEGKLHGETGYRQIVTNARGRQVAADSRELTHTL
                     LESESPEPGDNLYLSLDLDLQRVATEALQGQRGSVVAIDPTNGEILALVSAPSFDPNA
                     FALGMSASEYNALQFDLNQPLFNRAVRGNYPPGSLIKPMLALAALELGATNLTRRTFC
                     RGFYTLPGSTHRYRDWRPQGHGEIDLHDAIAQSCDVYFYEISREIGIEGMHTYLTQFG
                     LGSETGVDIAGEKTGLVPSREWKRQAFSDRPNQVWFPGETVIASIGQGYMLTTPLQLA
                     HATATLAMRGKRYRPHLVAATENPISGERDLAVPEELPGVMIENEFYWDSVISAMNAV
                     MQSETGTARASGLGAPYAMAGKSGTAQVFSVAQEDEYNESEVAERLRDHALFISFAPL
                     DDPQIAVAVVVENGSSGSRVAAPIARSVMDEYLGFGDDAI"
BASE COUNT          518 a          848 c          837 g          490 t
ORIGIN      
        1 cgggctgggc cgacaagcac aggacagaca ggaatacggc agatttcagt tttataagca
       61 ttgtccccgg acgcttcctg attcgcttgt ttactgtggc accagtttgc ggtgcgtgtg
      121 aatggacatg agaataccga atcccgccat gagggtcacc atggacgttc cgccgaaact
      181 caccagcggc aatggtatac cgactaccgg caccagaccg gtcaccatcg cggtattcac
      241 gaaaacatag acgaaaaacg tgaggcttat gctgcctgca agcaaacggg caaacgtctc
      301 gcgcgcctgt atcgcgatga acaagccgcg gccgacgacg aacatgtaga gcccgagcag
      361 gctcagtacg ccgaaaagcc ccagttcctc tcccatgacg gcgaaaatga agtcggtatc
      421 ccgctccgga agaaattcga gctgtgcctg gctgccgttc gcccacccct tgccgaacag
      481 gccacccgag ccgattgcga tctttgactg gatgatgttg tagccggctc ccagcgggtc
      541 gctctccgga ttcagcagca tcagcacacg gttttgctgg tactcgtgca tgaaacgcca
      601 caacagccac gcgcccggca ctgacaggag cccgaggatg atgatcaccc ggaactgcag
      661 gcccgcgagc accacgacca gcatgcccgc catggcgatg aggatcgatg tcccgaggtc
      721 gggctggacg gcgatcatta cggtgggtac tgcaattaac aacgcaatga gcaggaggtc
      781 tcccgcgcgc ggcggcaact ggcggtcgtg caggtaccac gcggcgacca tcggcaccgc
      841 tagcttcatg atttcggatg gctggaagcg gattcccagg tccagccagc gctgcgcgcc
      901 ctggcccacg tcgccgcgca gcaggacgag cgcgagaagc gcgagtccca gcgcatagcc
      961 ccacgccgtc cagcgtcgca tcacccccgg cggcacctgg gcaacgatga acagcaccac
     1021 caggccgatg ccgagccgga tgcattgctg cagccagagg tccatattct cgccaactgc
     1081 actgtagagc acgaccagtc ccaggcagca gatcagcaga aggccgccga ggagcgggcc
     1141 gtcgatgtgc aactggcgca aaaagcgcgc catgttgccg aggggcagcg aacggcgctc
     1201 gaaaagttct tcggtgccgc taaattgcat cgtcgccaaa tcccaggtac tcgtccatca
     1261 cgcttcgcgc gatgggggcg gcgacgcggc tgccgctgct cccgttttcg actaccactg
     1321 cgacggcgat ctgcggatcg tcgagcggtg caaaggaaat gaaaagcgcg tgatcgcgca
     1381 ggcgttccgc gacttcgctc tcgttgtatt cgtcttcctg cgcgaccgag aatacctgtg
     1441 cagtgccgct cttgcccgcc atggcgtaag gcgcgccaag gccgctcgcg cgcgcggtgc
     1501 cggtctcgct ttgcatcaca gcgttcatgg cgctgatgac gctgtcccag taaaactcgt
     1561 tttcgatcat gactcccgga agttcctccg gcaccgcgag gtcccgttcg ccggaaatcg
     1621 ggttttcggt agcggcgacg aggtgcgggc ggtagcgttt gccgcgcatc gcgagcgtgg
     1681 ccgtcgcgtg ggcgagttgc agaggagtcg tcagcatgta gccctggcca atggaggcga
     1741 tcaccgtctc gccgggaaac cacacctggt tcggccggtc gctgaaggcc tggcgcttcc
     1801 attcgcgtga cggtacgagc cctgttttct ctcccgcgat gtcgactccg gtttcgctgc
     1861 cgagcccgaa ctgggtgaga taggtatgca tgccctcgat gcctatctcc cggctgatct
     1921 cgtagaagta cacgtcgcag gattgcgcga tggcatcgtg caggtcgatc tcgccatgcc
     1981 cttgcggccg ccagtcgcgg taacggtggg tgctgcctgg aagcgtgtag aagccgcggc
     2041 aaaaggtgcg ccgggtaagg ttcgtggcgc cgagttccag cgcggcaagc gcgagcatcg
     2101 gcttgatcag ggatccaggt ggatagttgc cgcgcactgc ccggttgaac agcggctggt
     2161 tcaggtcgaa ctgcagcgcg ttgtactcgc tcgcactcat ccccagggca aacgcattgg
     2221 ggtcgaacga cggcgcgctc accagcgcca ggatttcccc gttcgtcggg tctattgcga
     2281 ccaccgatcc gcgctgcccc tgcagggctt ccgtcgcgac tcgctgcaag tcgaggtcga
     2341 gactgaggta gaggttatcg cccggttccg gcgattcgct ctccagaagc gtatgtgtaa
     2401 gctcgcggga atccgcagcc acctggcgtc cacgggcatt cgttacgatc tggcggtaac
     2461 ccgtctcgcc gtgcagtttc ccttcgttgt aaaattccac acccgtcttt ccggtatggg
     2521 ctgtgcctgc gtaagcggcg ggatcgagac gctcgaggtc gaccgcgctc agcgcgccga
     2581 cgtagccgat gacatgagcc gccagttccc cgtaaggata gtggcgcacc agcctcggct
     2641 ggaaatccac cccgggaaac ctgggccgct ggatcgcaaa gcgcgagatc tca
//
LOCUS       DASUOM010000437         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_291517,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000437 DASUOM010000000
VERSION     DASUOM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291517"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..463
                     /gene="arfB"
                     /locus_tag="VFY27_09055"
     CDS             47..463
                     /gene="arfB"
                     /locus_tag="VFY27_09055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5765702.1"
                     /translation="MLRISPDIDIPEHEIEIRAVRAQGAGGQNVNKVATAVHLRFDSQ
                     ASEVLTEEIKRRLLQSPDRRITDDGVVIIKAQRFRSQAKNRDDALSRLAEVIRAATRT
                     QRKRVPTKPSEAARRKRIDEKVRRGRLKESRGRIEE"
     gene            539..793
                     /locus_tag="VFY27_09060"
     CDS             539..793
                     /locus_tag="VFY27_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765703.1"
                     /translation="MTPKAVERIQTGVRLEKSLLKVLKGLAEYCDMSLGDLLEGIVLH
                     SFEGQPPFSDATLKTIRELKKVYNCSLTAADSHTLREDAS"
     gene            790..1221
                     /locus_tag="VFY27_09065"
     CDS             790..1221
                     /locus_tag="VFY27_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765704.1"
                     /translation="MSATQKRYSEGWECDPAKEVRRFEAGNIDPRKFDHEAHVRAAWS
                     YLQQYPAAVAIAKFTSALRSLTIRLGASDKYHETISWFFMIVIAERRAACPHADWQRF
                     RNTNRDLFEGSALLKRYYSKGRLDSAEARRHFLLPDLAPKN"
     gene            complement(1239..2915)
                     /locus_tag="VFY27_09070"
     CDS             complement(1239..2915)
                     /locus_tag="VFY27_09070"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX5765705.1"
                     /translation="MTAKTDLEIAQEARLLPIEEIGKKLGLSSDLLIPYGRDKAKIDY
                     PVLDNFEGRADGKLILVTAVSPTPAGEGKTTTTVGLGDALHAIGKNAAICLREPSLGP
                     CFGMKGGAAGGGYAQVVPMTDINLHFTGDFHAIGAAHNLLAALVDNHIHWENEPRIDP
                     RRVTWNRVVDMNDRALRNIVIGLGGRPHGVPRESGFDITVASEVMAIFCLASDLADLE
                     RRLANIVIGYTFDLQPVTVRDLDAQGAMMALLRDAFRPNLVQTMENTPALMHGGPFAN
                     IAHGCNSVVATRTALKLADYVVTEAGFGADLGAEKFFDIKCRKAHLKPSAVVLVATVR
                     ALKMHGGVAKENLTAADAKAVRKGCENLGQHLRNLGHFGVPIAVAINHFTADTEAELG
                     VIRNYCEEYGVEVHLCRHWAEGGKGASGLARHVVELVDGHPPQFHTLYDDEMPLWEKA
                     RHVARTMYGADDLIADKKVRDQFAQFQKDGYGKYPVCMAKTQYSFSTDPNLLGAPKGH
                     VVPVREIRLAAGAEFVVVVCGAIMTMPGLPRKPAAAGMRVNSSGQIEGLF"
     gene            complement(3103..>3891)
                     /locus_tag="VFY27_09075"
     CDS             complement(3103..>3891)
                     /locus_tag="VFY27_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5765706.1"
                     /translation="RFVIDPTTGALDESSEEVLLTVPQSAGNHNGGNIAFGPDGFLYA
                     GFGDGGGSGDPQGNGQDTTNLLATISRIDVDSATPYAIPAENPFAGNTECPRGAGTAD
                     CPEIFAWGFRNPWRFSFDRETGELWTGDVGENSWEEVDRVLVSENYGWSQREGAHCFD
                     PPTGCSTDNVDPIAEYPHDLGDAVTGGYVYRGSDIPDLEGFYLFGDFGSGRIWAVAAD
                     SEQGVEPGELVDTNLSIASFAESNEGELYALDIAAGTIHRIVAP"
BASE COUNT          736 a         1228 c         1194 g          733 t
ORIGIN      
        1 acgacgacgg gcacgcattt tcacacagtc atcgccggtt cacccaatgc tgcgcatctc
       61 ccccgacata gacatccccg agcacgagat cgagatccgc gcggtgcgcg cgcagggggc
      121 cggcggccag aacgtcaaca aggtggccac cgcggtgcac ctcaggttcg acagccaggc
      181 atcggaagtc ctgacggaag agatcaagcg ccgcctgctt cagtcgccgg atcgccgcat
      241 caccgatgat ggggtggtga tcatcaaggc gcagaggttc cgcagccagg cgaagaaccg
      301 tgatgacgcg ctctcccgtc tcgcagaagt cattcgcgca gccacgagaa cgcagaggaa
      361 acgggtgccg acgaagccga gcgaagcggc ccgcaggaag cgcatcgacg agaaggtgcg
      421 acgggggcgg ctgaaggagt cccggggacg aatcgaagag tgaatcccgc ttccggagag
      481 tagacatcgc gtggtctttg tactaatata ccttggtaac aaggtaacga gaaccgtcat
      541 gacaccgaaa gccgtggaac gcatccagac gggcgtccgt ctcgagaagt cccttttgaa
      601 ggtcctgaag ggactggccg aatattgcga catgtcgctc ggcgacctcc tggagggcat
      661 cgtgctgcac agcttcgaag ggcagccgcc attttccgac gccaccctga aaaccatcag
      721 ggaactgaag aaggtctaca actgctcgct caccgccgca gacagccaca cgctgcgcga
      781 ggacgcgtca tgagtgctac gcagaagcgg tactccgagg gatgggagtg cgatcctgca
      841 aaggaggtac gacggtttga agccgggaac atcgatccgc ggaagttcga tcacgaggca
      901 cacgttcgcg cggcctggtc atacctccag cagtatcccg ccgccgtcgc aatcgcaaaa
      961 ttcacctccg cgctgcgttc actgacgatc cggcttgggg cgagcgacaa gtaccacgaa
     1021 acgatcagct ggttcttcat gatcgtgatt gcggaacggc gggcggcctg tccgcacgcc
     1081 gactggcagc gcttcaggaa caccaatcgc gacctgttcg agggttcggc cctgctaaag
     1141 cgctattact ccaaaggccg cctcgattcc gcggaggcgc gccggcattt tctgcttccg
     1201 gaccttgcgc cgaaaaactg aggtacgctt ttccctgcct agaacaagcc ttctatctga
     1261 ccgctggagt tgacccgcat cccggccgct gcgggctttc gcggcagtcc cggcatggtc
     1321 atgatcgctc cacacacgac gacaacgaat tctgcgccgg ccgcaaggcg tatctcccgc
     1381 accggtacca cgtgtccctt gggagcaccg agcaaattcg gatcggtaga gaagctgtac
     1441 tgcgttttcg ccatgcagac gggatacttc ccgtaaccgt ctttctggaa ttgcgcgaac
     1501 tggtcgcgca ctttcttgtc ggcaatcagg tcatcagcac catacatcgt acgcgctaca
     1561 tgccgggcct tttcccataa cggcatctcg tcatcgtaca gggtgtggaa ctggggtggg
     1621 tgcccgtcga ccaactccac cacgtgacgg gcaagcccgc tggcgccctt cccgccttcc
     1681 gcccagtggc ggcaaaggtg tacctctact ccgtattctt cgcagtagtt cctgatgacg
     1741 ccgagttccg cctcggtgtc ggcggtgaag tggttgatcg caaccgcaat cggcacgccg
     1801 aaatgaccga ggttgcgcag gtgctgtccc aggttttcgc aacctttacg cacagccttc
     1861 gcgtccgcgg cggtgagatt ttccttcgcg acaccaccgt gcattttcaa tgctcttacg
     1921 gtcgcgacca gcaccaccgc cgatggcttc aggtgcgcct tcctgcattt gatgtcgaag
     1981 aatttctcgg cgccaagatc ggcgccgaaa ccggcttccg ttactacata gtccgcgagc
     2041 ttcagggcag tccgggtcgc gaccaccgag ttgcagccgt gggcgatgtt ggcgaatggt
     2101 ccgccgtgca tgagcgccgg ggtattttcc atcgtctgca ccaggttcgg gcggaaggca
     2161 tcccgcaaca gggccatcat tgcaccctgt gcgtcgagat cgcgtacggt gacgggctgc
     2221 aggtcgaaag tgtatccaat gacgatgttg gccaggcgcc gctccaggtc ggcgaggtcg
     2281 gacgccaggc aaaaaatggc catcacctcg gacgcgacgg tgatgtcgaa gccgctctct
     2341 cgcggaacac cgtgaggcct gccgccaagg ccaatgacaa tgttgcgcag cgcccggtca
     2401 ttcatgtcca cgacgcggtt ccaggtgacg cgccgcgggt cgatgcgtgg ctcgttctcc
     2461 cagtggatgt ggttgtccac cagggccgcc agcaggttat gagccgcgcc gattgcgtga
     2521 aaatcgcccg tgaagtgcag gttgatgtcc gtcatgggaa cgacctgcgc gtagccgccg
     2581 ccggccgcgc cgcccttcat gccgaaacag ggaccgaggc tcggttcgcg gaggcagatt
     2641 gcagcgttct tgccgattgc atgaagcgca tcgccgaggc cgaccgtcgt agtcgtcttt
     2701 ccctccccgg ccggcgtcgg cgacaccgcg gtcacgagga tcagcttgcc gtcggcgcga
     2761 ccttcgaaat tatcgaggac gggatagtca atcttcgcct tgtcgcggcc gtacgggatg
     2821 aggagatccg aggaaagtcc gagcttcttg ccgatctcct cgatgggcag caaccttgct
     2881 tcctgcgcaa tctcgaggtc cgttttcgct gtcattgttc aggctccatc ccggatgcgg
     2941 tgcggctgga agataccggg cgccgcgcgc gacggggacc cggtttcatg gttggcgggg
     3001 attctagcga aatcgccggg gaatacgcac ggattggcac tgcagtgccc catcacgtga
     3061 aacgagcgat ctgcatgagc agtggcgccg cactggtgcg ttttacggcg caacgatacg
     3121 gtgaatcgtc ccggcggcga tgtcgagcgc gtaaagttct ccctcgttgc tttccgcaaa
     3181 cgaggcgatg ctcaggttgg tatcgacgag ctccccgggc tcaacaccct gctcgctgtc
     3241 ggccgcgacg gcccagatcc ggcccgaacc gaagtcgccg aacaggtaga aaccctcgag
     3301 gtccgggatg tcgctgccgc gatagacgta gccaccggta acggcgtcgc ccaggtcgtg
     3361 cggatactcg gcaatgggat cgacattgtc cgtggagcag cccgtcggcg gatcgaagca
     3421 gtgcgcgcct tcgcgctggc tccatccata gttctcgctc accagcacgc ggtcgacttc
     3481 ctcccagctg ttttccccga cgtcgcccgt ccacagctcg cccgtctccc gatcgaagct
     3541 gaagcgccag gggttacgga atccccacgc gaaaatctcc gggcagtcgg ccgtccctgc
     3601 gccacgtggg cattccgtgt ttcctgcaaa ggggttctcc gccggtattg cgtacggcgt
     3661 cgcgctgtcg acatcgattc tcgagatcgt cgccagcagg ttggtcgtgt cctggccgtt
     3721 gccctgcggg tctccgcttc ctccgccatc gccgaagcct gcatagagga agccgtcggg
     3781 gccgaaagcg atgttgccgc cgttgtggtt gcccgcagac tgcggcacgg tcagcaggac
     3841 ctcctccgac gattcgtcga gcgcacccgt cgtgggatcg atcacaaaac g
//
LOCUS       DASUOM010000438         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_205787,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000438 DASUOM010000000
VERSION     DASUOM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205787"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFY27_09080"
     CDS             <1..852
                     /locus_tag="VFY27_09080"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5765707.1"
                     /translation="GRRRVVRFRAMFTYIDPTVRERLLAQGKLVRIDANGKVIGPDEE
                     LAAGEKAMSILGPIPMPIARSGRQFAVDWYASVRHTELSKVEELAEELRQRDGQQSFA
                     ILASSMAVNSVLVIGDPARWSNPLVRVHSSCLTGDVFGSARCECGPQFTAALERIADD
                     PEGGLLVYMSGHEGRGIGLWAKAATYVLQDAGEDTYQANRSLGLPDDSRDFSDAAALL
                     RYFLNDRPFRLLTNNPKKITDLNNYGVRNITPVKHVIGVSDANRRYLAAKRDWGHQLD
                     TDDLVVK"
     gene            complement(858..1679)
                     /gene="queF"
                     /locus_tag="VFY27_09085"
     CDS             complement(858..1679)
                     /gene="queF"
                     /locus_tag="VFY27_09085"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03138.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEX5765708.1"
                     /translation="MPDTLLGKRTTYPRRYARDVLRPIARADNRELLGVRSPLPFHGK
                     DIWNAYELTWLEPSGKPVVAVAAIEVDAASQAIVESKSLKLYLNSLAMERYGSAGDVA
                     ALIQRDLAETAQGDVLVTLATPSSIADAGIGDLPGIRIDDAAAYCDVFEVDPSLLERA
                     DAGTGGAVSEELHSHLLRSLCPVTDQPDFGSVLVRYRGAPIDRVSLLRYLVSYRNHND
                     FHESCVERIFVDLKERCDPEALTVHGFFNRRGGIDINPFRSDFEEAASKLRLWRQ"
     gene            complement(1719..>3054)
                     /locus_tag="VFY27_09090"
     CDS             complement(1719..>3054)
                     /locus_tag="VFY27_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765709.1"
                     /translation="VVMSDEDRIAAHSAIQKAVALKDGVTEPERDYIDALATRYNGDP
                     STPREPLDEAYAEAMRELYQKYPDDDDAASLFAESLMNTMPWDYWLDADHPKPRTVEV
                     LDALEAVLARTPDHPMAIHLYIHAVEASSRPERAEGPADRLADLVPGAGHLVHMPAHI
                     YWRVGRYHDASEANVKAAAVDESYIAQCNAQGFYPAMYYPHNIHFLWAASSMEGRSKV
                     AVDAARRVAKNVNLEMIDQFPGAEFFHTIPLLALTQFGLWDEVLAEPQPRADLEFSNG
                     IWHYVRATAFARKNDLAAARAEHAKLVPLKDATDVHFLDTIYYPATSLLEIADALVLG
                     EIALAEGNMDAAVEHFRTAVDTQDQLPYTEPPFWYYPTRHSLGRALLMKGDAAEAEEV
                     YRKDLEDYPHNGWAMYGLIQSLTAQGKDASEMQQMFDHAWSQADVTLTASRF"
BASE COUNT          576 a          927 c          971 g          580 t
ORIGIN      
        1 ggcaggcgtc gagtggtacg ctttcgcgcc atgtttactt acatcgatcc gacggtacgg
       61 gagcggctgc tggcgcaggg gaagctggtg cgcattgacg ccaacggcaa ggtcattggg
      121 ccggacgagg agctggctgc aggggagaag gcgatgagca tcctcgggcc gatcccgatg
      181 cctatagcgc gcagcgggcg gcagttcgcg gtggactggt atgcgagcgt gcggcatacg
      241 gagttgagca aggtggagga gctggccgag gagctgcggc agcgtgacgg gcagcagtcg
      301 ttcgcgattc ttgcgtcatc catggctgtc aatagtgtgc tggtcatcgg ggatcccgcc
      361 cgatggtcaa acccgctcgt acgcgtccat tcgagctgcc tgaccggcga tgtattcggg
      421 tctgcgcgct gcgagtgcgg tccccagttc actgccgcac tggaacgcat cgcagacgat
      481 cctgaaggag gcctgctggt ctacatgtca gggcacgagg gcaggggcat cggactgtgg
      541 gccaaggcgg ccacctacgt actgcaggat gccggcgagg acacttacca ggccaaccgt
      601 tccctgggat tgccggacga ctcgagagac ttcagcgacg cggcagcctt gctcaggtac
      661 ttcctgaacg accggccgtt ccgcctcctg accaacaatc cgaaaaagat caccgacctc
      721 aacaactacg gggtccgcaa cataaccccg gtaaagcacg tgatcggtgt cagcgacgcg
      781 aatcgccgct acctggctgc aaagcgtgac tggggccacc agctggatac ggatgacctc
      841 gtggtcaagt agccctgcta ctggcgccag agtcgcagct tgctggcggc ttcttcgaag
      901 tcggaacgaa agggattgat gtcgattcct ccacggcggt tgaaaaaacc gtgcaccgtg
      961 agtgcttcgg gatcgcatcg ctccttcagg tcgacaaaaa tgcgttccac gcagctttcg
     1021 tggaagtcgt tatggtttcg gtaggaaacc aggtagcgaa gcagggatac gcggtcgatc
     1081 ggcgctccac gatagcggac taagacgctg ccgaagtcag gctggtcggt caccgggcag
     1141 aggctgcgca gcaggtggga gtgaagctct tccgacacgg ccccgccggt gcccgcatcc
     1201 gctctctcca ggagggacgg gtcgacttca aaaacgtcgc agtaagcggc cgcatcgtcg
     1261 atgcggatgc cgggaagatc accaataccg gcgtccgcga tggaagaggg cgtcgccaag
     1321 gtaaccagta cgtccccctg cgctgtttcc gcaagatccc gctggatcag cgccgcgacg
     1381 tccccggcgg atccatagcg ctccatcgcg agcgaattca ggtacagctt gagtgacttc
     1441 gactcaacga tagcttggga tgccgcatcc acttcgatcg ccgcgaccgc cacgaccggt
     1501 tttcccgaag gctcgagcca cgtgagctcg taggcgttcc agatgtcctt gccatgaaac
     1561 ggcaaaggcg aacgtacgcc gagcagttcc cggttgtcgg cacgagcgat gggccggagc
     1621 acgtcccgcg cataccgacg cgggtacgtc gtgcgtttcc ccagcagcgt gtctggcatc
     1681 cgatgtgcta gccggggcta ttcatgaacg gtcccgagct agaacctgga tgcagtcagt
     1741 gtcacgtccg cctgtgacca ggcatggtcg aacatctgct gcatctccga cgcatccttt
     1801 ccctgcgccg tcaggctctg gatcaggccg tacatcgccc agccgttgtg cggataatcc
     1861 tcgaggtcct tgcgatagac ctcttctgcc tccgcggcgt ctcccttcat cagcagtgcc
     1921 ctgccgagag agtggcgcgt ggggtaatac cagaagggcg gctccgtata cggcaactgg
     1981 tcctgcgtat cgacggccgt gcgaaaatgt tcgaccgcag catccatgtt tccttcagcc
     2041 agtgcaatct cacccagcac cagtgcatcc gcaatctcaa ggagtgatgt agccgggtag
     2101 taaatcgtgt ccaggaagtg cacgtccgtc gcatccttca gcggcaccag cttcgcatgc
     2161 tcggcacgtg ccgcggcgag gtcgttcttt cgcgcgaaag cggtagcccg cacgtagtgc
     2221 cagatgccat tcgaaaactc gaggtccgca cgcggttgcg gctctgcaag aacctcgtcc
     2281 cacagaccga attgcgtcag ggcgagcagc ggtatcgtgt ggaaaaattc cgcacccgga
     2341 aactggtcga tcatttcaag attgacgttc ttggccaccc gccgtgctgc gtcgacagcc
     2401 actttgctgc ggccctccat gctggaggcg gcccacagga agtgtatgtt gtgcgggtaa
     2461 tacatcgcgg gataaaagcc ctgcgcgttg cactgcgcga tgtaggattc gtctaccgcg
     2521 gcggccttga cgttggcctc ggaggcgtcg tgataacgcc ctacgcgcca gtagatgtgc
     2581 gcgggcatat gaacgaggtg acctgccccc ggcacgaggt cggccagcct gtccgcggga
     2641 ccctcggcac gctccggacg cgacgaggcc tccacggcat gtatgtagag gtgaatcgcc
     2701 atcgggtgat cgggggtgcg cgccagcacc gcttcaagtg cgtccagcac ctcgaccgtc
     2761 ctgggcttgg gatggtcggc atcgagccag tagtcccacg gcatcgtgtt catcagggac
     2821 tcggcgaaca gtgacgcggc gtcgtcgtca tccggatact tctggtacag ctcgcgcatg
     2881 gcttctgcat acgcttcatc cagcggctcg cggggtgtcg atgggtcgcc gttgtaacgg
     2941 gtcgcgagcg cgtcgatgta gtcgcgctca ggctccgtga ccccgtcctt caaagcaact
     3001 gccttctgga tggcggaatg tgcggcgatc ctgtcctcgt cggacatcac gacc
//
LOCUS       DASUOM010000439         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_199733,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000439 DASUOM010000000
VERSION     DASUOM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199733"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="fabG"
                     /locus_tag="VFY27_09095"
     CDS             <1..691
                     /gene="fabG"
                     /locus_tag="VFY27_09095"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5765710.1"
                     /translation="GIGAAIAEALACAGARVIGTATSADGASRISAGLGERGRGAVLD
                     IADEESVQGLFADLQKLEGAPTIVVNNAGITRDNLLLRMKAEEWDDVLATNLSGVYRV
                     CKSSLRGMMKARYGRIVNIASVIGVMGNAGQANYAAAKAGIIGFSKSLAREVGSRAIT
                     VNVVAPGFIDTDMTRVLPEDQRQALLAQIPLGRLGETSDIADAVTFLVSDAGAYITGE
                     TLHVNGGMLMA"
     gene            1031..1273
                     /gene="acpP"
                     /locus_tag="VFY27_09100"
     CDS             1031..1273
                     /gene="acpP"
                     /locus_tag="VFY27_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5765711.1"
                     /translation="MSSIEEQVKSIVAEQLGVKEEEVTNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDFVTARRSES"
     gene            1455..>2380
                     /locus_tag="VFY27_09105"
     CDS             1455..>2380
                     /locus_tag="VFY27_09105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5765712.1"
                     /translation="MSKRRVVVTGMGIVSPVGSQLETAWENVREGRSGVAPIASFDAS
                     QFTTRIAGEVKDFDVEKYLQPRDARKIDKFMHYGIAASSDALADSGLEITAENSERIG
                     VAMGAGIGGLHTIESNHRKLLDFGPRKISPFFIPGSIINMVSGHVSIMHGITGPNIAI
                     VTACTTSTHCIGIAARTVAHGDADVMLAGGAEYATTPLSLGGFCAARAMTTRNDDPET
                     ASRPFDAGRDGFVLSDGAGCLVLEEYEHAQARGARIYAELIGFGMSGDAYHITLPPAD
                     GEGARKCMVAAMKDAAIDPSDVDYLNAHGTSTP"
BASE COUNT          505 a          673 c          776 g          426 t
ORIGIN      
        1 gggcataggc gccgcgatag ccgaggcgct cgcctgtgcg ggcgcgaggg taataggaac
       61 ggctacgagt gccgatggcg catcgaggat atcagcgggc ctcggagagc gaggccgcgg
      121 cgccgtcctc gacattgcag acgaggaatc ggttcaagga ctgtttgccg atttgcagaa
      181 gctggaaggc gcgccgacca tcgttgtcaa caatgccggc attacccgcg acaatctgct
      241 gctgcggatg aaggcggagg agtgggacga cgtacttgcg acgaatcttt cgggcgtcta
      301 cagggtctgc aaatcgagct tgcgcggcat gatgaaagcg cgatacgggc ggatcgtcaa
      361 cattgcgtcg gtgatcggag tgatgggcaa tgcgggccag gcgaattacg cggcggcaaa
      421 ggcaggcatc atcggctttt ccaagtcgct tgcccgcgag gtagggtccc gcgcgattac
      481 ggtaaatgtg gttgcaccgg ggttcattga taccgacatg acacgtgtcc tgccggaaga
      541 ccagcgacag gcactcctgg cacagatacc gctgggcagg cttggagaga cgtcggatat
      601 cgccgacgcg gtcactttcc tggtatccga tgcgggcgcg tacattaccg gcgagacctt
      661 gcatgtgaac ggcgggatgc tgatggcctg atggcggatg ccgggttggt cgacggcggt
      721 gtaattggcc gacgaagggc tctcggcgcc gccgcgcgtc cggcgccccg gtcacggtgc
      781 cgtgcgaacc cggcgtttgc cgggattgta gattgccaca ggggattcac atacaatacc
      841 gtcccgcggc gtggccgggg actttggata tcaagaactt acacctccgg caaggcgtcg
      901 cacccgatcc ggaagttgcc gaacgtcgct gtagcagcga tcgaaaggtg agctggaaac
      961 gcggcacccg aacaccggtt cagctggccc ggtttttttc gaagtcactt cctgaggaga
     1021 agcgatcact atgagcagta ttgaagaaca agttaaaagc atcgttgccg agcagttggg
     1081 ggtgaaagag gaagaagtca cgaatgacgc atccttcgtg gacgaccttg gcgccgattc
     1141 tttggacacg gttgaactgg tgatggctct cgaagaggaa ttcgaaaccg agattcccga
     1201 tgaagaagcg gagaagatca ccacggtcca gcaggctatc gacttcgtaa cggctcgtcg
     1261 cagcgaatcc tgaaaagcct ttttccaaca tgggcccagg gagcctctgc gcaagtctgg
     1321 acgaaaagct gtcgcacatg gcttgtgcac aggctcccta ggggcgcgac ggcaggacgc
     1381 gcccggatcc agtatggcaa tgcagtctcc ggcagacctg ccgggacaat cgtcacagtt
     1441 cccgggataa gtacttgagc aagcgacgcg tcgttgtgac cggcatgggt atcgtctctc
     1501 cggttggcag ccagctggaa acagcgtggg aaaacgtccg tgaagggcgc tccggcgtag
     1561 cgccgatcgc ctctttcgac gcaagccagt tcacgacccg gatcgccggc gaagtgaaag
     1621 attttgacgt agaaaaatac ctgcagcctc gcgatgcccg caagatcgac aaattcatgc
     1681 actacggcat cgccgccagc agtgatgcgc tcgccgattc cggcctcgag ataaccgcag
     1741 agaacagcga gcggatcggc gtggcgatgg gcgccggcat cggcggcctg cataccatcg
     1801 aatcgaatca tcgcaagctg ctcgacttcg ggccgaggaa aatctcgccg ttcttcattc
     1861 cgggcagcat catcaacatg gtgtccggtc acgtgagcat tatgcacggc attaccgggc
     1921 cgaacatcgc aatcgtcacc gcgtgcacca cgtcgacgca ttgcatcggc atcgccgcac
     1981 ggactgtcgc gcatggggac gcagacgtga tgctggccgg cggggccgag tacgcaacca
     2041 cgccgctgtc gctcggggga ttctgcgccg ctcgcgcgat gaccacgcgt aatgatgatc
     2101 cggagacggc aagccgtccc ttcgacgccg ggagggacgg cttcgtcctg agcgacggcg
     2161 cgggatgcct cgtcctggaa gaatacgagc acgcgcaagc gcggggggca cgcatttacg
     2221 ccgaactcat cggcttcggc atgagcggcg atgcctatca cattacgctg ccacccgccg
     2281 atggtgaggg cgcaagaaaa tgcatggtgg cagcgatgaa ggatgccgca attgatcctt
     2341 cggacgtcga ctacctgaat gcgcatggta cctcgacgcc
//
LOCUS       DASUOM010000440         4910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1378832,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000440 DASUOM010000000
VERSION     DASUOM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378832"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFY27_09110"
     CDS             complement(<1..1078)
                     /locus_tag="VFY27_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5765713.1"
                     /translation="MIAFSILLLLLAVLGAPLFTVIAASAMLGYQQEGIDLMAIAIEI
                     LGIAEMPFLSAIPLFTFAGYLLSESKAPRRLVRLTNAMLGWVPGGLALVALAACAFFT
                     AFTGASGVTIIALGAILYPALQQDGYPDNFNLGLVTSGGSLGLLFAPSLPLILYGVIA
                     EVPIDGLFRAGVLPGLLMLVMLAGWSFWVNRHSRKPPGSFSWREVGGALRESLWELPL
                     PFVVLGGIYSGFFAVSEAAAVTALYVLVVEVFILREIKVTDLPLIMRDSMLLVGGILI
                     ILGVSLASTNYMIDAGVPQKLLAVVAELVTSPASFLVLLLIFLLILGAILDIFSAIVL
                     VVPLILPIADQYGIDPFHLGIIFLA"
     gene            complement(1075..1593)
                     /locus_tag="VFY27_09115"
     CDS             complement(1075..1593)
                     /locus_tag="VFY27_09115"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5765714.1"
                     /translation="MNDKGSRPLERLRQFGTSVENALLVLLLSAMMLLAVGQIVLRMF
                     FNSGLLWADELLKILVLWIALVASVAAARSRRHLRIDILTRYVPQRYAQVPELVVDLF
                     AAAICGFIAWHSLQFILLSMGDTVLVNVPSWVAQGIVPAAFLLMCYRFTLHFLSGVLG
                     LARGNRARTPAP"
     gene            complement(1617..2645)
                     /gene="dctP"
                     /locus_tag="VFY27_09120"
     CDS             complement(1617..2645)
                     /gene="dctP"
                     /locus_tag="VFY27_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5765715.1"
                     /translation="MRHPRLVTAVMLLVALVTAQAGAADLKIATVLPENTQWMKDMRA
                     GAAEIRERTGGRVNFKFYSGGVQGSANKVLRKIKIGQLHGGAFTPTDLQQVYPDLNIY
                     GLPFLFESEEEVNYVRERVDPILMEGLEQAGFVSFGFSSCGFAIIMSNVPVRTHDDLK
                     GRKIWVPEGDLISYETMKGLQLSPVTLPVTDVLTGLQTGLIDIVAMSPAGALVLQWHT
                     KVEYLTKLPILYSMGLLAIDGRAFKRIDETDQAVVRDVMGRIYEEWDAENQQDAENAL
                     QALIKSGIEAVEPAPGEQEKLQDLMQRQNRIMADKGLFSEALLEQVLTHISTYRSEFD
                     SDESVAAH"
     gene            complement(2745..3656)
                     /locus_tag="VFY27_09125"
     CDS             complement(2745..3656)
                     /locus_tag="VFY27_09125"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX5765716.1"
                     /translation="MSVFAGMRTLPPRQLLRGSGRVRVTLLAAAAMLAAGGCASLMSS
                     AATGVAENLSVAVLNQTDPETVRDGAPAYMLLLDSFLEESPDDPELLSAAANLYASYG
                     SVFADDPDRARRLTERARQYASRAVCVSYSESCDWQDMSFDEFESTLEGLEPRHAGAV
                     YSYSIASLAWIRAHSEDWNALAELPQMNALLSRYLDIGGGEMRGSVYTYLGILATLRP
                     PSLGGEPEKGREYFERAIELTNGRDLSVKVEYARGYARLLYERELHDRLLHEVMEADP
                     DVPGYTLTNVLAQRDAAELLAGADEYF"
     gene            complement(3740..4084)
                     /gene="rplS"
                     /locus_tag="VFY27_09130"
     CDS             complement(3740..4084)
                     /gene="rplS"
                     /locus_tag="VFY27_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5765717.1"
                     /translation="MSKIIEAIEQEQMTKEVPDFSPGDTVVVQVKVTEGERERLQAFE
                     GIVIAKRNRGLNSSFTVRKVSHGEGVERVFQTYSPTVSAVEVKRRGDVRRAKLYYLRD
                     RTGKAARIKEKI"
     gene            complement(4195..4908)
                     /gene="trmD"
                     /locus_tag="VFY27_09135"
     CDS             complement(4195..4908)
                     /gene="trmD"
                     /locus_tag="VFY27_09135"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5765718.1"
                     /translation="MVPKIAGYGVVGRAVTRRLVTLGCQNPRDFAKDAHRTVDDRPYG
                     GGPGMVMKFEPTAAALEAAKRSAPAGSLVVCLSPQGPVFDQAVARRYAELPGLVLLAG
                     RYEGIDERLIETQVDEELSIGDFVLSGGELAAMVVIDAVTRLLPGVLGDEDSAEQDSF
                     TDGLLDHPHYTRPEVVEGRKVPEVLLSGDHARIARWRYKQALGRSFLRRPDLVRKLHL
                     NDEQQRLLHEFLRDQESVQ"
BASE COUNT          995 a         1587 c         1416 g          912 t
ORIGIN      
        1 ctgcgaggaa gattattccg aggtggaacg ggtcgatccc gtactgatcg gctatcggca
       61 ggataagcgg cactaccagt acgatcgccg aaaagatgtc gaggatagcg cccagaatca
      121 gcaggaatat cagcaacagg accaggaaac tcgccggact ggtgacgagt tccgccacga
      181 cggcaagcag tttctgcggg acaccggcat cgatcatgta gttggtggat gcgagcgata
      241 cgccgaggat gatcaggatg ccgcctacca ggagcatcga gtcccgcatg atgagcggca
      301 ggtccgtcac cttgatctcg cgcagtatga atacctcgac caccagcacg tagagcgcag
      361 tcactgccgc ggcttccgag acggcgaaga agccggaata aatgccaccc agcaccacga
      421 aaggcagtgg caattcccaa agcgactctc gaagcgcacc gcccacttca cgccaggaga
      481 aactgcccgg cggtttgcgg ctgtgccggt taacccagaa gctccaaccc gccagcatga
      541 ccagcatcag gaggcccggc agaacaccgg cgcggaacag gccgtcaatc ggcacttccg
      601 ctatgacgcc gtaaagtatc aatggcagcg acggggcgaa cagcaggccc aggctgccgc
      661 cgctcgtcac cagccccagg ttgaagttat ccgggtaacc gtcctgctgt aacgccggat
      721 agaggatcgc gccgagcgca ataatggtga caccggaagc gccggtaaag gccgtgaaga
      781 aggcacaggc ggcaagggca acgagggcaa ggccacccgg cacccagccg agcatggcgt
      841 tcgtcaggcg aacaaggcgc cgcggcgcct tgctctcgct cagcaggtac ccggcgaacg
      901 taaagagcgg aatggccgag aggaagggca tctcggcgat tcccaggatc tcgatggcga
      961 tggccatcag gtcgatacct tcctgctgat agcccagcat cgcgctggcg gcaatcacgg
     1021 tgaatagcgg cgcaccaaga accgcgagaa gcagcagcaa tatgctgaac gcgatcacgg
     1081 cgcgggtgtc cgcgcacggt ttccacgggc gagaccgagg acccctgaca ggaagtgcag
     1141 cgtgaaccgg tagcacatca gcaggaacgc ggccggcacg atcccctgcg ccacccatga
     1201 cgggacgttg accaggaccg tatcgcccat ggaaagcagg atgaactgca gcgagtgcca
     1261 ggcaatgaaa ccgcagatgg cggccgcaaa caggtcaacc acgagttccg gaacttgtgc
     1321 atatcgttgc ggcacgtacc ttgtcaggat atcgatgcgc aagtggcgcc ggctgcgtgc
     1381 cgccgcaacg gaagcaacga gcgcaatcca cagtacgaga atcttgagaa gctcgtccgc
     1441 ccagagcaga ccggaattga agaacatcct gagaacgatc tggcccacgg ccagcaacat
     1501 catggcgctg agcaacaaca ccagcagggc gttttcgacc gaggtgccga actgcctcag
     1561 gcgttcgagc gggcgcgacc ctttgtcatt caccacggga acccgtggcc agccccttaa
     1621 tgcgccgcga ccgactcgtc cgagtcgaat tcacttcgat aggtgctgat gtgcgtcagt
     1681 acttgctcga gcagggcttc ggaaaaaagc cccttgtcgg ccatgatgcg attctgccgt
     1741 tgcatcaggt cctgcagttt ctcctgctcg ccgggcgccg gctcgaccgc ctcaatcccc
     1801 gacttgatga gtgcctgcaa cgcgttctcc gcatcctgct ggttttcggc atcccactcc
     1861 tcgtaaattc gccccatcac gtcccgcacc accgcctggt cggtctcgtc gatgcgcttg
     1921 aaggcgcggc cgtcgatcgc gagtaagccc atggagtaca gaatcggcag ttttgtcagg
     1981 tactcgacct ttgtatgcca ttgcaagacc agcgcgcccg ccggcgacat cgcaacgatg
     2041 tcgatgagac cggtctgcag tcccgtgagc acgtccgtga caggcagcgt aaccggtgac
     2101 agctgcaggc ccttcatcgt ctcgtagctg atcagatcgc cttccggtac ccagattttc
     2161 cgtcccttca ggtcgtcgtg ggtgcgtacc ggcacattcg acatgatgat ggcgaatccg
     2221 cagctcgaaa agccgaagct gacgaacccg gcctgctcca ggccctccat caatatcggg
     2281 tcaacgcgtt cccgcacgta gttgacctcc tcttccgact cgaagaggaa cggcaggccg
     2341 tagatgttca ggtcagggta aacctgctgc aaatccgtcg gtgtgaatgc cccgccgtgc
     2401 aactggccga tcttgatctt gcgcagcacc ttgttggcgc tgccctgtac gccgccggaa
     2461 tagaatttga aattgacccg tccgcccgtt cgctcgcgga tctccgcggc accggcacgc
     2521 atgtccttca tccattgggt gttctccggc aggaccgtcg cgatcttgag atccgccgca
     2581 cctgcctgtg ccgtgacaag tgccacgagc agcatcactg cggttaccag tctgggatgt
     2641 cgcatactcg ttctccgaat atatccatca agcataacaa gagttcgttt tcgtcactgc
     2701 gccagtcgcg atgcggaaaa ggtcgcctgt gcaagatacc gccgtcagaa atattcgtct
     2761 gcgccggcga gaagttcggc cgcatctcgt tgtgcgagca cattggtcag cgtgtaaccg
     2821 ggcacgtcgg gatctgcctc catcacctcg tgcaacaggc ggtcgtgcag ttctctttcg
     2881 tacagcagcc gcgcgtatcc ccgcgcatac tccaccttta cgctcaggtc ccgtccattg
     2941 gtcagttcta tcgcgcgttc gaagtattcc cggccttttt ccggctcgcc tccaagcgac
     3001 ggcggcctga gcgtagcaag gatgccgagg taagtataaa cactgcctct catttcaccg
     3061 ccgccgatgt cgaggtagcg gctcaacagg gcgttcatct gcggcagctc cgccagcgcg
     3121 ttccagtctt cgctgtgagc gcgaatccac gcaagcgagg cgatggaata gctgtagacg
     3181 gcaccggcgt gtcgaggttc gagtccctcg agcgtcgact cgaactcgtc gaagctcatg
     3241 tcttgccagt cgcaggattc cgagtagctc acgcacaccg ccctgctcgc gtattgccgc
     3301 gcccgctccg tcagccgccg cgcccgatca ggatcgtctg cgaagaccga accgtaggat
     3361 gcgtagaggt tcgccgccgc agacagcaac tccggatcgt cgggactttc ttcgaggaag
     3421 ctgtcgagga gcagcatgta agccggcgcc ccgtcgcgga cggtctccgg atccgtctgg
     3481 ttcagaacgg caaccgagag gttctcggct actccggtcg cggcagatga catgagcgat
     3541 gcgcaaccgc cggctgccag catagcagca gccgcaagca aggtgacgcg tacccgcccc
     3601 gagccacgca ggagttgacg gggcggaagc gtcctcatgc cggcaaagac actcatgctc
     3661 tgatttggtt caggcttccg cgcagccgca cggccggtcc agctgctggc cggcgagcac
     3721 gcattcatgg cttgccgaac tagatcttct ctttgattct cgcagcttta ccggtgcggt
     3781 cacggaggta gtaaagcttg gcgcggcgca catcaccgcg gcgcttgacc tcgacggcac
     3841 tgaccgttgg gctgtatgtc tggaataccc gctcgacgcc ttcaccgtgc gacaccttgc
     3901 gcaccgtgaa cgaagaattg agtccccgat tgcgcttggc aatgacgatg ccttcgaaag
     3961 cctgcagccg ttcacgctcg ccttcggtaa ccttcacctg tacgacgacg gtatcgccag
     4021 gcgagaaatc cggcacttcc ttggtcattt gttcctgttc gatcgcttcg attattttgc
     4081 tcatgtttct ctacctgcca actttgttgg actcaagaat tcaaccggta accgttacgt
     4141 tacccgtgcc ggcgccgcct gctgtcggca gagtctgcgc agcaggaagt gttcctactg
     4201 gacagactcc tgatcccgta aaaactcatg gagcagccgt tgctgctcat cgtttaggtg
     4261 caacttgcgc accaggtcag gccggcgcag gaaactgcgc cccagcgcct gcttgtatcg
     4321 ccatcgcgct atccgcgcat gatcgccgga cagcagtacc tccggcactt tccggccctc
     4381 taccacttcg ggccgcgtgt aatgcggatg atccagcaga ccgtccgtaa acgaatcctg
     4441 ctccgcggaa tcctcgtcgc ccagcacccc ggggagcagc ctcgtcaccg cgtcgattac
     4501 caccatcgca gccaactcgc cgccgctcag gacgaagtcc ccgatcgaga gttcctcgtc
     4561 cacctgcgtc tcgatcaatc gctcgtcgat gccctcatac cggcctgcga gcagtaccag
     4621 tccgggcaat tccgcatacc ttctcgccac ggcctgatcg aacaccggtc cctgcgggga
     4681 caggcacact accaggctcc cggcgggtgc ggaccgcttc gccgcttcta atgcagccgc
     4741 ggtcggctcg aacttcatca ccatgcccgg gccgccgccg taggggcggt cgtctaccgt
     4801 gcgatgcgca tccttcgcga aatcgcgtgg attctggcat ccgagcgtca ccagtcgccg
     4861 ggtcaccgca cggccaacga cgccgtaccc ggcaatcttt ggcaccatct
//
LOCUS       DASUOM010000441         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_772993,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000441 DASUOM010000000
VERSION     DASUOM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772993"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFY27_09140"
     CDS             <1..1063
                     /locus_tag="VFY27_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEX5765719.1"
                     /translation="EIALRVSAIAAGSARLYASLGVWDAVAAVRASPFREMRVWDASH
                     SADGAEALRFDAAEFALPELGFIVENVLLQDALLRLVEKAGVTVEFGTQIEALRYPDN
                     STEVVCGNETLHADLVIGADGVSSAVRQQARIGVTSWRYGQSALVTHVRPERDHGECA
                     WQRFLPEGPIALLPLLDGRSSVVWSTSPEQAQEAQAADDRTLGLMLTEASGRVLGDLS
                     VAGDRAIFPLKAQYAVRYVRRGTALIGDAAHSIHPLAGQGANLGIADASVLARTLKAA
                     IEAGEYPGDLPVLRRYERARKGANATMLYFVDALNRLFGAPSAPARAVRSAGMRAFNL
                     SGPIRRRAVAVALGLQPGP"
     gene            1246..2766
                     /locus_tag="VFY27_09145"
     CDS             1246..2766
                     /locus_tag="VFY27_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HEX5765720.1"
                     /translation="MTTRYVLVLAGILTLVGLATFIYKWQVLGLPLDASQETPTWTVE
                     TTIRFDSGPGSISVNLQIPTLTPGFATLDEFSVSRNYAFGLNYVGADREAQWTVRRAD
                     GEQTLYYRIVVYEDPESDQSDLTPPLPIPPVINEPFRTAVDMVVADVSEHSADAASFT
                     SVLLRMFNDPSPNPNIELLLADASSRADFVQTLTTVLASAAIPARVIRGIPLQEQQRQ
                     ADPVPWLEIHDGDRWRYFDPDTGAEGLPERFLIWWRGSEPLVRVEGGSNVEVHFAVQR
                     NFIDSLSIAEHRAEVRQSRVIDFSLFSLPIETQSVYGVLLMIPIGAFIMVLMRNVIGI
                     DAFGTFMPVLIALAFRETQLLWGVILFSVLVALGLSIRFLLERLRLLLVPRLSAVLII
                     VVLLMLVISIVSHQLGMETGLSVALFPMVIIAMTIERMSVVWEERGAGDALRAGAGSM
                     LVATIAYVVMGLPWLEHMVITVPELLLVVLAAVLLLGRYTGYRLSELTRFKAIIGK"
BASE COUNT          509 a          857 c          921 g          542 t
ORIGIN      
        1 cgaaatagcg ctgcgcgtgt ctgcaatcgc tgcaggctcg gcgcggctat acgcatcact
       61 gggtgtctgg gatgcagtcg ccgccgtgcg tgccagcccg ttccgggaga tgagagtctg
      121 ggacgccagc cacagcgcgg atggtgccga agcgctgcgc ttcgacgccg cggaattcgc
      181 attgccggaa ctcggcttca tcgtcgagaa cgtgctgttg caggatgcgc tcctcaggct
      241 cgtggagaaa gccggcgtga ctgtcgaatt cggaacgcaa atcgaggcgc tgcgatatcc
      301 cgacaacagc accgaagtcg tttgcggcaa cgagaccctg cacgcggatc tcgtcatcgg
      361 cgccgatggc gtttcgtcgg ccgtacggca acaggccaga atcggcgtaa cctcctggcg
      421 ttatggccag tcggcactgg ttacccacgt ccgtcccgag agggatcacg gcgaatgcgc
      481 atggcagcgg ttcctgcccg aaggcccaat cgcactgctg ccgcttctgg atggccgcag
      541 ctcggttgta tggtcgacgt cgccggagca ggcacaggaa gctcaggctg cggacgaccg
      601 gacgctcggc ctgatgctga cggaggcaag cggccgtgta ctgggcgatc tgtcggtggc
      661 gggggatcgg gccatctttc cgctcaaggc gcagtacgcg gtgaggtatg tcaggcgagg
      721 tacggcactc atcggcgacg ccgcccacag catccatccg cttgcggggc agggggccaa
      781 cctggggata gcagacgctt ccgtgcttgc gaggactctt aaagcagcca ttgaagcagg
      841 ggaatatccc ggagacctgc cggtcctgcg ccgctacgaa agggcgcgca agggggccaa
      901 tgcgacgatg ctgtatttcg tggatgccct gaaccgcctg ttcggggctc cctcggcgcc
      961 ggcgcgtgcc gtgcgttccg cgggtatgcg cgccttcaac ctgagcggac cgatacgtcg
     1021 ccgtgctgtc gcagtcgccc tcgggctaca gccgggtccg tgaccgagga tgggcggact
     1081 ccaataagcg ttaaaggccg acgcattccc gtgtagaatc tgcgtccccc gggggatgcc
     1141 tgccgggata tcgaagaaaa agaaccaggc ggcacgatct gtcccggaac cagaaaagca
     1201 aaagagcaag ccattgaaat cccgaaccgc gcttaaggca acgcagtgac cacccgttac
     1261 gtactggttc tcgcgggcat cctcacgctc gtcgggttgg ctaccttcat atacaagtgg
     1321 caggtactgg ggttgccgct ggacgcatcc caggaaacgc cgacatggac ggtagaaacg
     1381 acgatccgct tcgacagcgg tcccggctcg ataagcgtca atctgcagat ccctacactg
     1441 acgcccggct tcgccaccct ggatgaattt tcggtgtccc gaaattacgc attcggtttg
     1501 aattacgtgg gcgcggatcg cgaggcgcag tggacagtgc gccgggccga tggcgaacag
     1561 acgctttact accgaatcgt cgtctatgaa gacccggaga gcgaccagtc ggacctgacc
     1621 ccgccgctac cgattccgcc ggtaatcaac gagccgttca ggacggctgt ggacatggtg
     1681 gtggccgacg tcagcgaaca ttctgccgat gccgcatcgt tcacttccgt gctgctcagg
     1741 atgttcaacg atccctcgcc gaatccgaac atcgagctct tgctcgcgga cgccagctcg
     1801 cgcgccgatt tcgtccagac cctgacgacg gtgcttgcgt cggccgccat cccggcgcgg
     1861 gtgatccggg gcatcccgct gcaggaacag cagcggcagg cggacccggt gccttggctg
     1921 gaaatccacg acggcgaccg ctggcgatac ttcgatcccg acaccggcgc ggaagggctg
     1981 ccggagagat tcctgatctg gtggcgcggc agtgaaccgc tggtcagggt agagggcggc
     2041 agtaacgtcg aagtacattt cgccgtgcag agaaacttta tcgactccct ctcgatcgcg
     2101 gagcatcgtg ccgaggtgag gcagtcccgc gtgatcgact tttcattgtt cagcctgccg
     2161 atcgagacgc agtcggtcta tggtgtgctg ctgatgatcc ccatcggcgc cttcatcatg
     2221 gtgctcatgc gcaacgtaat aggcatcgat gccttcggta ccttcatgcc cgtgctgatt
     2281 gcgctggcgt ttcgcgaaac tcaactgctg tggggcgtca tactgttctc ggtcctcgtg
     2341 gcgctgggtc tcagtattcg gttcctgctg gagcgcctgc ggcttctgct ggtgccgcgg
     2401 ctgagcgccg tcctgatcat cgtggtgctc ctcatgctgg tcatcagcat cgtctcgcac
     2461 cagcttggaa tggagaccgg gttgtccgtg gcactgttcc caatggtcat catagcgatg
     2521 acgatcgagc ggatgtcggt cgtatgggag gagcgaggag cgggcgacgc attgcgcgcc
     2581 ggcgccggca gcatgctggt cgcgacgatt gcgtatgtgg tgatggggct gccatggctc
     2641 gagcacatgg tcatcacggt cccggaactg ctgctcgtcg ttcttgcggc cgtcctgctg
     2701 ctcgggcgtt acaccggcta ccgattgtcg gaactgaccc gcttcaaggc gatcatcggc
     2761 aaatgaaggg gccgcactga catgctcggc aaccgcaaac tgctgcggga agccggcgtg
     2821 ctcggcctg
//
LOCUS       DASUOM010000442         4622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1660201,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000442 DASUOM010000000
VERSION     DASUOM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660201"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFY27_09150"
     CDS             <1..432
                     /locus_tag="VFY27_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765721.1"
                     /translation="RVVPAGAEAADLGVDALIEPSIDAFEFSVPNQSQTDAFAVWIRY
                     RIKIFDGEGNQIANWPISAYGKSMSEGIGNDDALRRAAVLAMRDAAALVILQMDKATG
                     ISALAEARRSSVAAQPPATLESAPAENLHTITQEDPRDDTG"
     gene            419..1114
                     /locus_tag="VFY27_09155"
     CDS             419..1114
                     /locus_tag="VFY27_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765722.1"
                     /translation="MIPANQSPTGWNLFRGTTLAAIGLTMSACVTSRVEDMRTSDTGL
                     REGEAVVIMAKSYHLGNETEADFISCVGDSIGRGSDGLRVVPNSEFVDALFPWFEPRT
                     APAETKSLPDLMQRPGVPEMIAEKGIRYVVWLDGATDRVAGGGSMSCAAGPGGGGCFG
                     FAWWQNDSKYEASVWDLKGLEDAGTVSADFSGTSFLPAIVVPIPLLARTQSKACKGLA
                     DQLRVFIVSDENV"
     gene            1367..2641
                     /locus_tag="VFY27_09160"
     CDS             1367..2641
                     /locus_tag="VFY27_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765723.1"
                     /translation="MRSIQNSALAFGLAMVLSACSVSTSEAEAQEPASERLYRLDYVV
                     RPDYAAKGVHVELTVKQAGYWLREMDMPLRDGQISRVSGDGNISTEGDRAVWSIPKRG
                     GKLRWFALINNKRNGDSYDAYIDRDWALFRGMDAIPSARTKALRRAESRTSLRFDLPD
                     GWSSATPYYGRDDGYEINNPDRSFDTPTGWIVLGEIGVRNERIGHVLAKVAGPTGHSI
                     RRMDMLALMRWNLPELLRVLPDFPERLTFMSAGDPMWRGALSAPDSFYIHADRPLISE
                     NATSTMLHETVHVGLGLSAEAGADWIVEGLAEYYSLEMLRRSGTIGERRYRRAHAGLQ
                     AWGRDVKELCTRRSSGSNTARAVTIISEVAGEISKLSGGKSSIDDVLRALASYEEKIT
                     VEKFREIVADIAGEPVEALKTKTLPGCRGTPQ"
     gene            2688..4178
                     /locus_tag="VFY27_09165"
     CDS             2688..4178
                     /locus_tag="VFY27_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5765724.1"
                     /translation="MDRQRDLELILKSSMPLVVIETRDESGMLGMLQNISLSRSKDDY
                     LPLFRWTITDGLQRLDISLEPQLHNAKPTEVLRHIRAVTKPGVYVLLDFHPYLQDPVN
                     VRLLKDICLRYAEVPRQLVLVSHQIGIPDDLRGYSARFEMALPTEKEREAIVRRAATQ
                     WASDNPGSRMSIDPKAYRLLIRNLAGLTDADTERLARNAIWSDGAISKSDLPAVMQAK
                     YELLNSGGILSYEYDTAKFADVGGLQNLKKWLMQRKAAFLSASGAPHLDAPKGVLLIG
                     VQGCGKSLAAKASAGVFGVPLLRLDFGALYNKYHGETERNLRETLKTADVMAPCALWV
                     DEIEKGIAGRDSEAGTSQRVLATFLTWLAEKKNRVFVVATANDISALPPELVRKGRFD
                     EIFFVDLPSLPIRAEIAGIHLVNRGQQRQRFDLKQIAEATEGFSGAEIEQAIVSALYE
                     AHAEKEALATRHVLEAALTTRPLSVVMAENIAALRQWSAGRTVSSD"
     gene            4267..>4622
                     /locus_tag="VFY27_09170"
     CDS             4267..>4622
                     /locus_tag="VFY27_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765725.1"
                     /translation="MANDNKKINDLVSENDDTSELEVLTATVVFDRSQDDDYESESDA
                     ATHSFESPARATGSDDASIAALKSDLQKREENIHNLQYDIAELRARWTGLEKELRARE
                     ELTDNVTSELEQSNRKL"
BASE COUNT          995 a         1404 c         1427 g          796 t
ORIGIN      
        1 cgggtcgtcc cggcgggcgc agaagccgct gatctcggcg tcgacgcact gatcgagccg
       61 agcatcgacg cgttcgaatt ttccgtaccc aaccaaagcc agacagatgc tttcgcggta
      121 tggatacgct accgcataaa gattttcgac ggcgagggca accagatcgc taactggccg
      181 atcagtgcct acggcaagag catgagcgaa ggaatcggta acgacgatgc gctgagacgg
      241 gctgccgtgc tggctatgcg cgacgcggct gcgctcgtga tcctgcagat ggacaaggcc
      301 accggcataa gcgcgctggc cgaagcgcgc cgctcaagcg ttgcggcgca accaccggcg
      361 acgctcgagt cagcgcccgc cgaaaacctg cacacgatta cccaggagga tccgcgagat
      421 gataccggct aatcaatctc ccaccggatg gaacctcttc cgtggtacca cgcttgccgc
      481 cataggcctg accatgagcg cctgcgtgac ctcacgcgtc gaggacatgc gaaccagcga
      541 caccggctta cgcgaaggtg aagccgtcgt catcatggcg aagagttatc acctcggtaa
      601 tgaaacagag gcggacttca tatcttgtgt gggtgattcg atcgggcgcg ggtcagacgg
      661 cctgcgggtc gttcccaaca gcgaatttgt cgacgcgctg tttccctggt tcgaaccgcg
      721 aaccgccccc gctgaaacca agagtctgcc agacctgatg cagcgccccg gggtgccgga
      781 gatgatcgcc gagaaaggga tccgttacgt tgtctggctt gacggggcga ccgatcgggt
      841 cgccggtggc ggcagcatgt cctgcgcggc aggccccggc ggcggcggct gcttcggctt
      901 tgcctggtgg cagaatgatt cgaagtacga agcgtccgtc tgggacctca aaggactgga
      961 agacgccgga acggtcagcg ctgatttcag cggcacctcg ttccttcccg caatcgtcgt
     1021 gccgattccc ctgctggccc ggacgcaatc gaaagcctgc aaggggctgg ccgaccagct
     1081 tcgggtcttt atcgtcagcg acgaaaacgt ctgagacgtt ttatataaga agtccaagca
     1141 atccttagcc gggccgcccc tccgcggacg aacgtcctgc agtagaactc gcctgtcgtc
     1201 gggccgggcc gcgccacccg cgcgggcccg cggcggccaa ggtgcttcct tgatccgctg
     1261 gatgctttcg gcgacctgtg gaacactgcc cgctttttat gccgctccac ttgatactcc
     1321 cagggcttcg ccgatcatga accacctccc gtcaaggaaa agactgttgc gttcaataca
     1381 aaactccgcc ctggccttcg gtctggcaat ggtgctgtca gcttgcagcg tgtcgacctc
     1441 cgaggcagaa gctcaggaac ctgcctctga acgcctctat cgcctggact acgtcgtccg
     1501 accggactac gcagcgaaag gggtgcacgt ggaactgaca gtgaagcagg cgggatactg
     1561 gctcagggaa atggacatgc cgctgcggga cggccagatc agccgtgtca gcggcgatgg
     1621 aaacatatcc acggagggcg atcgagccgt atggtcgata ccaaagcgcg gcggcaagct
     1681 gcgctggttc gcgctcatca acaacaagcg gaacggcgac agctacgacg cctatatcga
     1741 tcgcgactgg gccctgtttc gcggcatgga cgccattcct tccgcaagaa cgaaagcttt
     1801 gcggcgggcc gaaagccgca ccagcctgag attcgacctg cctgacggct ggtcgtcggc
     1861 aacaccttat tacggtcggg atgacggcta cgaaatcaac aatccggacc gcagtttcga
     1921 tacgcccacc ggttggatcg tcctgggcga aatcggggta cgaaatgaga ggatcggcca
     1981 cgtactggcc aaagtcgccg gccctaccgg tcattcgatc aggcgcatgg acatgctcgc
     2041 gctgatgcgc tggaacctcc ccgaactcct gcgagtgttg cccgactttc ccgagcggct
     2101 gaccttcatg agcgccggcg acccgatgtg gcggggggcg ctttctgcgc cggattcgtt
     2161 ttacatccat gccgaccggc cgctgatcag cgagaacgca acgagcacca tgctacacga
     2221 gaccgtgcac gtcggcctgg ggctctcggc cgaagctggc gcggattgga tcgtcgaggg
     2281 gctcgctgag tactacagcc tggagatgct gcggcgaagc ggcactatcg gcgagcggag
     2341 ataccggagg gctcatgcgg ggctgcaggc gtggggtcgg gacgtgaagg agctttgcac
     2401 acgccggtcg agcggcagca acacggcccg ggccgtcacc atcatctcgg aggtagccgg
     2461 cgagatcagc aaactgagcg gcggcaagtc gagcatcgac gatgtgctgc gcgcacttgc
     2521 aagttacgag gaaaagatca cagtggaaaa gttccgcgaa atcgttgcgg acattgccgg
     2581 ggaaccggtc gaggcgctga agacgaagac actgcccggc tgccggggaa cacctcagta
     2641 ggagcccgcg ccggggggat ccggtaacgc ccctcaaaaa agcgagaatg gacagacaac
     2701 gcgacctcga actgatcctc aagtcgagca tgccactcgt ggtcatcgag acccgtgatg
     2761 agtccggcat gctaggcatg ctgcagaaca taagcctctc ccgttcgaaa gacgactacc
     2821 tgccgctgtt tcgctggacg atcaccgacg ggctgcaaag gctggacatt tccctcgagc
     2881 cacagctgca caatgccaag ccgacggagg tgctgcggca cattcgggcg gtaacaaaac
     2941 cgggcgttta cgtgctgctg gactttcatc cctacctcca ggatccggtg aacgtgcgtc
     3001 tgttgaagga catctgcctg cgctacgcgg aagtacccag gcaactggtg ctggtgagcc
     3061 accagatcgg tataccggac gacctgcgcg ggtatagcgc gcgcttcgag atggcactgc
     3121 cgacggaaaa agaacgtgaa gcgattgtcc gacgcgcggc tacgcaatgg gcctccgata
     3181 accccggttc gcgtatgtct atcgatccga aagcctaccg actcctgatc cggaacctgg
     3241 ccggacttac tgatgccgat acggagcgtc tcgcacgaaa cgccatctgg tccgacggtg
     3301 cgatcagcaa gagcgacctt cccgcagtga tgcaggcaaa gtacgagttg ctgaacagcg
     3361 gcggcattct cagttatgaa tacgacacgg cgaagtttgc cgacgtcggg gggctgcaga
     3421 acctgaagaa atggctgatg cagcgaaaag cagcgttcct ttcggcgtcc ggcgcgccgc
     3481 acctcgacgc accgaaaggc gtcctgctga tcggagtcca gggctgcggg aaaagccttg
     3541 ccgcaaaggc gagcgcaggc gtcttcggag tgcccttgct gcgcctcgac ttcggcgcgc
     3601 tgtacaacaa gtaccatggc gaaaccgagc ggaatctccg cgaaacgttg aaaacagccg
     3661 acgtcatggc tccgtgcgcc ttgtgggtcg acgagataga gaaaggaata gccggccgcg
     3721 acagtgaagc cggtacatcg cagcgcgtcc tggcgacatt cctgacatgg ctcgcggaaa
     3781 agaagaaccg ggtattcgtg gtcgcgacgg cgaacgacat cagcgctctg ccgccggagc
     3841 tggtgcgcaa gggacgcttc gacgagatct ttttcgtcga cctgccctcc ttgcccatcc
     3901 gtgccgagat cgcaggtatc cacctcgtca accgcgggca gcagcgccag cgatttgact
     3961 taaagcagat agccgaggcc actgaaggct tctccggtgc cgagatcgag caggctatcg
     4021 tctccgccct gtacgaagcc cacgcggaaa aagaggccct tgcgaccagg cacgtgctgg
     4081 aggctgccct gacgacccga ccgttgtcgg tggtcatggc cgagaacatt gccgcactta
     4141 ggcagtggtc tgccggccgc acggtctcct ccgactgatc cgccactaaa gtcatccgca
     4201 catgtcctct ggcccccttc cttaaggggt ctggacttgc gatccgcttt gggacaccat
     4261 accgaaatgg ctaatgacaa caaaaaaatc aatgatttag tctccgaaaa cgacgacacg
     4321 tcggaactcg aggtacttac cgcgacggta gttttcgacc gcagccagga cgacgactac
     4381 gagtcggaat cagacgcagc aacccattcc ttcgaaagcc cggcccgggc gacaggttca
     4441 gacgatgcgt ccatcgcggc gctgaagtcg gacctgcaga aacgcgagga aaacatacac
     4501 aatctgcagt acgacatcgc ggaattgcgg gcgcgctgga caggcctcga gaaagagctg
     4561 agagcgcgcg aggaactcac cgacaacgtc acgtcggaac tggagcagtc aaaccgcaag
     4621 ct
//
LOCUS       DASUOM010000443         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_410982,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000443 DASUOM010000000
VERSION     DASUOM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410982"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFY27_09175"
     CDS             complement(<1..397)
                     /locus_tag="VFY27_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765726.1"
                     /translation="MTLQSRLNLGILGIAIVLVAPLVLSLRALQDIQTESTLLLDREF
                     AATLLLGRMRVALDEIELNATYIPVYNSDSSQKSFLDQLTTLARRTDTLTVLTESAIV
                     GQIRSSTARVFEQAPAAFELARANRVARRD"
     gene            504..1802
                     /gene="asnS"
                     /locus_tag="VFY27_09180"
     CDS             504..1802
                     /gene="asnS"
                     /locus_tag="VFY27_09180"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX5765727.1"
                     /translation="MTTFSSISNLAQHAGSTVTVRGWVSHLRSSGKIAFIVLRDGSGL
                     LQAVLVKSQVAPEVWERFPQLTQETSLAVTGEVRAEARAPGGYEMGVTGVEVLGSSPT
                     DYPIQPKEHGIDFLLDNRHLWLRSPRQVAILRVRHEIEQAIHDFFYQRGFIHVDTPVL
                     TAAIGERSGLFQTEYFDEGFAYLAQTGQLYGEAAAAALGCIYTFGPTFRAEKSKTRRH
                     LTEFWMIEPEMAFYDTHDNMRLQEDMLLYIVRRVLERRQAELAVVERDVSKLERITGP
                     FPRIEYTDAVEILQKKGSGIKWGDDLGAEDESLLVEDHETPVFVCNYPKEAKAFYMKE
                     NPADPRTVLCDDCLAPEGYGEIIGGSQREDDHDKLVERIKEEGLPMDAYGWYLDLRKY
                     GTFVHSGYGLGLERTVAWICGTPHIRECAAFPRMMNRLRP"
     gene            1891..>2186
                     /locus_tag="VFY27_09185"
     CDS             1891..>2186
                     /locus_tag="VFY27_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX5765728.1"
                     /translation="MSIAASFIERSRYYLCREYPVKIRLAVSSMPGDRLWWRPNESAN
                     SVGNLLLHLSGNVRQWIVSGVGGQGDVRKRNMEFAARGGATASEMLDGLDVTIR"
BASE COUNT          425 a          637 c          711 g          413 t
ORIGIN      
        1 agtcgcgtcg ggcgacgcga ttggcgcgcg cgagttcaaa tgcggccggt gcctgctcga
       61 ataccctggc ggtcgatgac cggatctgcc ccacaatcgc cgactcagtg agcaccgtga
      121 gcgtatcggt tcttcgggcg agcgtggtca gttggtccag gaaggacttc tggctgctgt
      181 cgctgttgta gaccgggatg tatgtcgcgt tcaactcgat ttcatccagt gcgacgcgca
      241 tgcgaccgag gaggagcgtt gcggcgaact cccggtcgag aagtaacgtg gattcggtct
      301 ggatatcctg caaagctcgc aatgacaaaa ccagcggcgc gaccagaacg atcgcgatgc
      361 cgaggatgcc gaggtttaac cgagactgaa gggtcacacg ggcgctcgct ctggttacga
      421 gttactggtt actggtattg ctactgtccc gatttccatc ctcacgctct acctttccgt
      481 ctcactcttc cgctcgataa cgcgtgacaa ccttttcgtc gatctcgaat ctagcccagc
      541 acgctggttc caccgtgacc gtgcgcggct gggtgtcgca cctccgatct tcaggcaaga
      601 tcgccttcat tgtcctgcgt gacggttcgg gtttgctgca ggcagtcctg gtcaagtccc
      661 aggtggcgcc ggaggtctgg gaacggttcc cccaattgac gcaggagacc tcccttgcgg
      721 taacggggga agtacgcgcg gaggcccggg cgcctggcgg ctatgagatg ggcgtcacag
      781 gggtggaggt gctgggaagc agcccgaccg actacccgat ccagccaaag gaacacggca
      841 tcgacttcct gctcgataac cggcatctct ggctccgcag tccacgccag gtcgccattc
      901 tccgcgtcag gcacgaaatc gagcaggcca tacacgactt cttctaccag cgcggcttca
      961 tccacgtgga tacgcccgtc ctgacagccg cgatcggcga acgaagcggg ttgttccaga
     1021 cggagtattt cgacgaagga ttcgcctacc tggcgcaaac ggggcagttg tacggcgagg
     1081 cagcggctgc cgcgctcggc tgcatctaca ccttcgggcc gacgttccgg gcggagaagt
     1141 caaagacgcg ccggcatctt accgagttct ggatgatcga gccggagatg gcgttttatg
     1201 acacgcatga caacatgcgg ctgcaggaag acatgctgct gtacatcgtc cggcgggtcc
     1261 tcgagcgtcg tcaggcagag ctggcggtcg tggagcgcga tgtgtcaaag ctcgaacgca
     1321 ttacggggcc gttcccacgc atcgagtaca ccgacgccgt cgagatcctc cagaagaagg
     1381 gcagcgggat caagtgggga gacgacctcg gcgccgaaga cgagtcgctg ctggtcgagg
     1441 accacgaaac gccggttttt gtctgcaact atccgaagga agccaaggcg ttctacatga
     1501 aggagaatcc ggcggatcct cgcaccgtgt tatgtgacga ttgcctggcg ccagaaggat
     1561 acggtgaaat catcggcggg tcgcagcgcg aagacgacca cgacaagctc gtggagcgca
     1621 tcaaggagga ggggctgccg atggacgctt acggctggta cctcgacctc cggaagtacg
     1681 ggacgttcgt tcactcaggg tacggcctcg gcctcgagcg aaccgtggcg tggatttgtg
     1741 gcaccccgca catccgcgaa tgcgcggcgt ttccgagaat gatgaatcgg cttcggccgt
     1801 aagggggtac ttcagaccgg cgctgacttc caaacggcgc tgacttccaa acggcgctga
     1861 cttagacggc gcgggccggt ggggaacatc atgtcgattg cagcttcatt catcgagcgt
     1921 tcacgctact acctttgccg cgaatacccg gtgaagatcc gactggctgt ctcctcgatg
     1981 ccgggcgacc ggctgtggtg gaggcctaac gagagtgcca acagcgtcgg caacctgctc
     2041 ctgcacctgt cgggcaacgt gcgacagtgg atcgtgagcg gcgtcggggg gcagggggac
     2101 gtgcgcaaac ggaacatgga gtttgccgcc cgcggcgggg cgaccgcgtc ggagatgctc
     2161 gatggtctgg acgtcacgat acgcga
//
LOCUS       DASUOM010000444         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1419413,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000444 DASUOM010000000
VERSION     DASUOM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419413"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..>3177
                     /locus_tag="VFY27_09190"
     CDS             569..>3177
                     /locus_tag="VFY27_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5765729.1"
                     /translation="MLKADKDNSRRAAPYTRRYARWLVLMTGFGLTLVLGLTAAVIGA
                     YYYVAPGLPQAETIREIPLQIPLRIYSRDGRLIDEIGERRRIVVGYDDLPQYVVDAFV
                     AAEDGRFFEHPGVDYRGIVRAAVSFLRHGDVSEGGGGSTITQQLARDYFLTRDVLFTR
                     KLREAFLAYKIEKEFSKQEIMALFVNKMFFGQRAYGVAAAAQVYYGKGLQNMNIAEAA
                     TIAGVLPSPSYYNPVTSPTNALMRRNYVLRRMHDLNFISEVELEEAMNYPLESRLYGT
                     KNELSAPWVAEMARREMLERYGEDAYSTGYKVVTTLDSRLQAAANYSLRNGLLEFSRR
                     RGYRGPITNVSGSVDLQVLDEPYAEWPDELKLMLTEYGNPGGLAVGMVTALDEQDRSA
                     TVVLQNGRREKLPWRGISWAKPFIDREAFGPAPKTVAEVLAPGDVVYLMPTTAGTYAL
                     AQVPEAQAAVVSVDPVDGAITSLAGGFDFSASKFNRVTQAARQPGSSFKPFIYSAALE
                     QGNTPATVILDAPVVINSSELEAVWRPHNYTGRFYGEQRMREALVRSMNLASVRLLLN
                     NTGIGNAVRHIAKFGFGDAALPRNGSLALGGGNATPLDMAQGFAVFANGGYAVKPYII
                     DTISDADGDILYRAEPAVVCRECEGGLDDIEGMRAALTPDQFAATTAERYEEGYDEDA
                     EEPMTLDQMAAVGESYRPDATVAPELFRGIKLAPRVIPEENAYLVSDMMRDVIRRGTG
                     RRALALGRSDLSGKTGTSNDRRDAWFGGFNADLSSVVWVGYDDDLPLGPGEEGSRTAL
                     PVWVEFMRVGLKGARENALPMPEGIVSVRISKSTGCPATAAVPLEDVMFEIFDQDDVP
                     VCDTPDNQSDIFNT"
BASE COUNT          609 a         1000 c          984 g          584 t
ORIGIN      
        1 caaagcgatt taactatata tcaacaaaat gttaaataaa acgtgacttt gtccacgatc
       61 cggcggcgcc tatggtactt ccggaagttc tccggattcc gtgaactggt tggcgctggt
      121 aagagacagt tctcccccct ccccaaatct gcctgttccc cggatgatct ttttgaccgg
      181 tccgccggcg tggtcgtctt tacagggtct ggatgccccg gttttttgcc cctgccaccc
      241 gtcgcagagc cctcgcggcg gccgggcatc atcacagctt accccgctgt agccgagcag
      301 cgacagtagc tccgtcacat tccctcacca tgcaggaact ggttcgcaac ctgtgcgtcc
      361 ggcccttgag gacctgacag aagccggtat gctggctatc atcccggcga acgagtcgct
      421 gtcgctcaac aacgaggcta tcccggaacg ctgccggccg cgctgatgca atgaacgctt
      481 taacgcgcag taagtccatc cctgcgcccc aactcgtcct ctgacttatt cctaacttca
      541 ggacctctca cccgacacag accccgatat gctgaaagcc gacaaggaca attcgcggcg
      601 ggccgcgccc tacacacgtc gctacgccag gtggctcgtg ctgatgaccg gcttcgggct
      661 gaccctggtg ctggggctga ccgcggccgt gatcggggct tactactatg ttgccccggg
      721 actgccgcag gccgagacga tccgcgagat cccgttgcaa attccgctcc ggatctacag
      781 tcgtgacggg cgattgatcg acgagatagg cgagcggcgt cgcatcgtcg tcggctatga
      841 cgacctgccg caatacgtcg tcgacgcgtt cgtggctgcc gaggacgggc gtttcttcga
      901 acatcccggc gtcgactatc gcggcatcgt gcgcgccgcg gtcagtttcc tgcgccacgg
      961 cgacgtgagc gaaggcggtg gcggcagcac gatcacccag cagctcgcgc gggactattt
     1021 cctgacccgc gacgtgctgt tcacgcgcaa gttgcgggaa gcgtttctcg cttacaagat
     1081 cgagaaggag ttcagcaagc aggaaatcat ggccctgttc gtgaacaaga tgttcttcgg
     1141 ccagcgagcc tacggggtag cggctgcggc gcaggtctac tacgggaagg gcctgcagaa
     1201 catgaatatc gccgaagcgg cgaccatcgc cggcgtgttg ccttcgccgt cgtattacaa
     1261 cccggtaacg agcccaacga atgcattgat gcggcgcaac tacgtcctcc gacgcatgca
     1321 cgacctgaat ttcatcagcg aggtggaact ggaagaggcc atgaactacc cgctggagtc
     1381 gcggctctac gggaccaaga acgagttgtc ggcaccgtgg gttgcggaaa tggcccgtcg
     1441 cgagatgctc gagcgctacg gcgaggacgc gtactccacc ggctacaagg tagtcaccac
     1501 gctcgactca aggctgcagg ccgctgccaa ttattcgctg cgcaacgggt tgctggaatt
     1561 cagccgtcgc cgcggctatc gcgggcctat caccaatgtc tccgggagcg tcgacctcca
     1621 ggtgctcgat gagccgtatg ccgagtggcc ggatgaactg aagctcatgc tgacggagta
     1681 cggcaatccc ggcggcctcg ccgtcggcat ggtgaccgca ctcgatgagc aggacaggag
     1741 cgctaccgtg gtcctgcaga acggccgccg ggagaaactg ccgtggcgcg gcatttcctg
     1801 ggcgaagccc ttcatcgacc gggaagcctt cgggcccgcg ccgaagacgg ttgcggaggt
     1861 gctggcaccg ggagatgtcg tgtacctgat gccgaccacc gccggcacct atgcgctcgc
     1921 ccaggttccg gaggcacagg ccgcggtggt ttcggtggac ccggtggatg gcgccatcac
     1981 aagcctcgcc ggcggtttcg atttctccgc cagcaagttc aaccgtgtga cgcaggcggc
     2041 acggcaaccc ggctcttcgt tcaagccttt tatatattcg gctgcgctgg agcagggaaa
     2101 cacgccggcg acggtcatcc tcgatgcccc ggtggtcatc aattcgtccg aactcgaagc
     2161 cgtatggcgt ccgcacaact acacagggcg cttttacgga gagcaacgca tgcgcgaagc
     2221 gctggtgcgt tcgatgaacc tggcgtctgt gcgactgctg ctgaacaaca ccggcatcgg
     2281 taacgcggtc cgccatatcg cgaagttcgg cttcggcgat gccgccttgc cccgaaacgg
     2341 ttcgctcgcg ctgggcggcg gcaacgccac acctcttgac atggcgcaag gatttgccgt
     2401 gttcgcgaac ggcggctatg cggtgaagcc ttacatcatc gacaccatca gcgatgcgga
     2461 cggtgacata ctgtaccgag ccgaacccgc ggtcgtttgc cgcgagtgcg aaggcgggct
     2521 tgatgatata gagggaatgc gcgcggcatt gacgcccgac caattcgccg caacgacggc
     2581 tgagcgatat gaagaaggct atgatgagga cgccgaagag ccgatgacgc tggaccagat
     2641 ggccgcggtc ggcgaatcct accggccgga cgccaccgtc gcgccggaac tgttccgcgg
     2701 catcaagctc gcgccgcgcg tcatcccgga agagaacgcc tacctggtct cggacatgat
     2761 gcgggacgtc atccgtcgcg gtaccggcag gagggcactt gcactggggc gcagcgacct
     2821 gtccggcaag acgggcacct cgaacgaccg gcgcgacgcc tggttcggcg gcttcaacgc
     2881 cgacctctcc tccgtcgtct gggtgggtta cgacgacgac ctgccgctcg ggcccggaga
     2941 ggaaggctcc cgaaccgcgc tgccggtctg ggtagaattc atgcgcgtgg gcctgaaagg
     3001 cgcgcgtgaa aacgcgttgc ccatgcccga gggcatcgtc agcgtacgca tttcgaagtc
     3061 gaccggttgt ccggcaactg cggccgtgcc actcgaagac gtcatgttcg aaattttcga
     3121 ccaggatgac gttcccgtct gcgacacgcc ggacaaccag agcgatatat tcaacac
//
LOCUS       DASUOM010000445         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1015822,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000445 DASUOM010000000
VERSION     DASUOM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015822"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFY27_09195"
     CDS             complement(<1..214)
                     /locus_tag="VFY27_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5765730.1"
                     /translation="MVDVSDKRATDRVAHARAIVEFPEAVAERFVDGDIATAKGPVFA
                     TAIIAGVMGAKRTHDLIPFCHPLGLDN"
     gene            complement(300..1358)
                     /gene="moaA"
                     /locus_tag="VFY27_09200"
     CDS             complement(300..1358)
                     /gene="moaA"
                     /locus_tag="VFY27_09200"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5765731.1"
                     /translation="MTATVLPDPAHAKTTAPRDALGRPMRDLRISLLDRCNFRCPYCM
                     PEAEFNADYQFLRKPELLTHAEILRIAKVAVSLGVTKLRLTGGEPLLDKTVPALVESL
                     SKLHGVEDLALTTNGTLLAPMAAQLARAGLHRVTVSLDSLDEKIFREMSGNRGDLQKV
                     LQGIDAADNAGLSPVKINTVVQRGVNDHTVMDLLEHFRGSGHIVRLIEFMDVGNRNDW
                     KMEQVVPSRELLDRIQERWPVRAIGRSYPGEVASRYEYEDGAGQIGFISSVTEPFCGA
                     CSRARLSADGVLYTCLFATTGTSLRDPLRSGADDAGLAEILGNAWLRRKDRYSELRRP
                     DATREHVMKKVEMYRMGG"
     gene            complement(1502..2260)
                     /locus_tag="VFY27_09205"
     CDS             complement(1502..2260)
                     /locus_tag="VFY27_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5765732.1"
                     /translation="MVYLALAFFFVAFLYASVGFGGGSTYTALLIESGLDWTLVPPVS
                     LMCNIVVVAGGVYHFAKAGHIDLKLATPLIIASIPAAFFGGYLRLSETSFLLILGAAL
                     LIAGGLLVFDRSFRDETSAMTMSPAGSGLFLGALLGGLAGVTGIGGGIYLAPVMHLFR
                     IAPARIVAATCSLFILVNSVSGLAGQVMKLGAAAEPLMRAQYLALPVAVFVGGQLGSR
                     AAAKWLPVSPIRRLTGAVIVLVALRILWQATTGD"
     gene            2464..3666
                     /locus_tag="VFY27_09210"
     CDS             2464..3666
                     /locus_tag="VFY27_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5765733.1"
                     /translation="MNVNYSEDELAFRDQVRTFLQEKYPADLREKQSKGIALGKDDIV
                     RWQKILYEQGWAAVNWPVEYGGTGWTAVEKHIFANELAAANAPEMIPFGVKMVAPVIY
                     TFGNDAQKSRFLPDILQSNVWWCQGYSEPGAGSDLASLKTRADRHGAHYVVNGTKTWT
                     TLAHFADWIFCLVRTSSDVARRQEGISFLLVDMSTPGVTVKPIITIEGEREVNEVHFE
                     NVEVPVGNLVGEEGKGWTYGKVLLQHERTNIAGVARSKYRMRRLRQKAAQSVHGGAPL
                     LDDRNFARRLAAIEIELKALEYTELRTLAAVATGKAPGPESSILKVKGTELQQAIDAL
                     SVDAAGYYALPYVPEQYQADYPVGEMVGSGTETQTSLRYFNNRKASIYGGSNEIQKNI
                     ISKHVLGL"
BASE COUNT          778 a         1139 c         1172 g          695 t
ORIGIN      
        1 agttgtcgag cccgagggga tgacaaaagg ggatcaggtc atgcgtccgc tttgcgccca
       61 tcacgccggc aataattgca gtagcgaaga ccggcccttt tgcggtcgcg atatcaccat
      121 ccacgaaccg ctctgcgacg gcctcgggga attccacgat ggcacgcgcg tgcgcgacgc
      181 ggtccgttgc gcgcttgtcg ctgacgtcga ccatcgtcgg ccgattccgg ctgtctacgt
      241 ggctcagctt gctcaaatgc ggttcctgtg gcgcggaatc gtcatagata ccggtggcgt
      301 cagccgccca tccggtacat ctcgaccttc ttcatcacgt gctcacgagt cgcgtccggg
      361 cgtctcagct cgctgtagcg gtctttgcgc cgcagccaag cgttgccgag gatctccgcg
      421 agccctgcgt cgtcggcgcc gctgcgcagc ggatcccgga gactcgttcc ggtcgtagcg
      481 aaaagacagg tatagagaac gccatcggcc gagagccggg cgcggctgca tgcgccgcag
      541 aacggttccg tcaccgagga tataaagccg atctgccccg cgccgtcctc gtattcgtag
      601 cggctagcca cttccccggg ataacttcgg ccgatggcgc gaaccggcca gcgctcctgg
      661 atgcgatcga gaagctcccg cgacgggacg acctgttcca tcttccagtc attgcggttg
      721 ccgacgtcca tgaattcaat gagacgcacg atgtgtccgc tgccgcggaa atgttcgagc
      781 aggtccatta ccgtgtgatc gttgacgccc cgctgcacca cggtgttgat cttcactggc
      841 gagaggcccg cattgtcggc agcgtcgata ccctgcagaa ccttctgcag gtcgccgcga
      901 ttgccgctca tttcgcgaaa gattttttca tccagggaat cgaggctcac cgtcacgcgg
      961 tgtaatccgg cgcgcgccag ctgcgccgcc atcggcgcca gcagcgttcc attcgtggtc
     1021 agcgcgagat cctcgacacc gtgaagtttg ctcaacgatt ccacgagtgc cggcaccgtc
     1081 ttgtcgagca gcggttcgcc gccggtcagg cgaagtttcg tgactccgag gctcaccgcc
     1141 accttcgcta tcctgaggat ctcggcgtgc gtcagcagct ccggcttgcg caggaactgg
     1201 tagtccgcat tgaactccgc ttccggcatg cagtaaggac agcggaaatt gcagcgatcg
     1261 agcagggaaa tgcgcagatc tcgcatgggg cggcccaggg catcgcgcgg agcggtcgtt
     1321 ttcgcgtgag cgggatctgg aagtacggtc gcggtcatat gatgcatttt tgcatgaaat
     1381 gaggatggct gcgaaggcgc ctggggcctg gatcggcctg cgcaatcgtt ctgaccgaac
     1441 gcaggtggtt agttccgcat tccccgccgg gcccgcccag gctgtcgctt gattccggtt
     1501 ctcaatcccc ggtcgttgcc tgccacagga tgcgcaacgc caccaggact atgacggcgc
     1561 cggtgagccg cctgatcggc gataccggca gccatttagc cgctgcgcgg cttcccagct
     1621 ggccgccgac gaataccgca accggcaagg ccaggtactg agccctcatc agcggttccg
     1681 ccgcggcgcc gagcttcatg acctggcccg ccagcccgga gacggaattc accaggatga
     1741 acagcgagca ggtggcggcg acgattctcg cgggggcaat cctgaacagg tgcatcaccg
     1801 gcgcgaggta gatgccgccg ccgatgccgg tcactccagc caggccaccc agcaacgcac
     1861 caagaaacaa cccggagccg gcagggctca tcgtcatcgc gctcgtttca tcgcgaaaac
     1921 tgcgatcgaa cacgagcagc ccacccgcaa tgagaagagc cgcgccaagg atcaggagaa
     1981 aagaagtttc actgagcctc aggtagccgc cgaagaacgc tgcgggaatg gaggcgataa
     2041 tgagcggcgt cgcaagcttg aggtcgatat gcccggcctt cgcgaaatgg tagacaccgc
     2101 ctgcaaccac gacaatgttg cacatcagcg atacgggtgg cacgagcgtc cagtcgagcc
     2161 ccgattcgat caaaagtgcc gtataggtcg atccgccgcc gaaaccgacg gatgcataga
     2221 ggaaggcaac gaagaaaaat gcaagcgcaa ggtagaccat tgcggaacct caggtacttg
     2281 ttgttgtttg cataacgccg ccccgttgcg cgcgctgcga agtgcgacgg cacatcggat
     2341 atactgcgac gacggtccgc gcaactacgc aaaatgccgg cgggccaata ttagcgcgga
     2401 ttcccgtcgc tcgatgactg agaatgctgc tggtcggcag cgacgcgata ccagggtttg
     2461 atcatgaacg taaattattc tgaagatgaa ctggcatttc gtgatcaggt acgcaccttc
     2521 ctgcaggaga aatatcctgc ggacctgcgt gagaaacagt caaagggcat cgcgcttggc
     2581 aaggatgata tcgtccgctg gcagaagatc ctgtacgagc aaggttgggc cgcggtgaac
     2641 tggccggtcg agtatggcgg cactggctgg actgcggtcg agaagcacat attcgcgaat
     2701 gaactcgcag cggcgaatgc gccggagatg atcccgttcg gggtcaagat ggtcgcgccg
     2761 gtgatatata ccttcggtaa cgatgcccag aagtcgcgat ttctccccga cattctgcag
     2821 agcaacgtgt ggtggtgcca gggttattcc gagcccggcg ccggctcgga cctagcttcg
     2881 ctcaagacgc gggcagatcg tcatggcgcc cactatgtcg taaacggcac caagacctgg
     2941 accacgctcg ctcactttgc cgactggatt ttttgcctcg tccgcaccag ttcggatgtc
     3001 gcgcggcgcc aggaagggat cagtttcctg ctggtcgaca tgagcacacc cggagtcacg
     3061 gtgaagccca tcatcaccat cgagggcgag cgcgaggtca acgaggtgca tttcgaaaac
     3121 gtcgaagtgc cggtcgggaa cctggtcggc gaggaaggga aaggctggac gtacggcaag
     3181 gtactgctgc aacacgagcg taccaacatt gccggtgtcg ctcgctcgaa ataccgcatg
     3241 cgccggctgc ggcagaaagc tgcgcaatca gtgcatggcg gggctccctt gctcgacgac
     3301 cgcaattttg ctcgcaggct cgccgcgatc gagatcgagc tcaaggcgct tgagtacacc
     3361 gaacttcgta ccctggcggc agtcgccacc ggcaaggccc cgggaccgga atcgtcgatc
     3421 ctgaaggtga aggggaccga actgcagcag gcgatcgatg cgctgtccgt cgacgcggcg
     3481 ggctattacg cgctgccata cgtgccggag caataccagg cggattaccc ggtgggcgag
     3541 atggtcggtt caggcaccga gacgcaaacg tcgcttcgtt acttcaataa ccgcaaggcc
     3601 tcgatctacg gcgggtcgaa cgagatccag aagaacatca tttccaaaca cgtgctcggg
     3661 ctctagttgt gccccgttgt aaataatgga acgttggcgc gagcgatttt tcgtgactga
     3721 caaggcgcga cgacgagtaa tagcaccgct attgtgagga gtcgcaactc cgccagacgc
     3781 gaaa
//
LOCUS       DASUOM010000446         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1304222,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000446 DASUOM010000000
VERSION     DASUOM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304222"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFY27_09215"
     CDS             <1..425
                     /locus_tag="VFY27_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765734.1"
                     /translation="LNYLTKWKSQQVPGSPVVDYKGSGAEAGLYDYLPFTTFSYRNDR
                     LSVGLNWRYLPAIEHSSLPDNPTSTQQGAGSYSYFSLNGGWRFNETMRLRAGIDNLFD
                     QEPEIYGRTDENNAGITTLTGRYDPLGRRGFVALEVNF"
     gene            520..1452
                     /locus_tag="VFY27_09220"
     CDS             520..1452
                     /locus_tag="VFY27_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5765735.1"
                     /translation="MPSRRSVLTAAVGGAAGVSLGLLPGFRSFVAQAQSGANRTLDLG
                     GGLHVLTVGQTNVVARTGAEGVMLIDGGAAAQSAALSALISALPATGKVHTLFNTHWH
                     PEQTGLNEALGRAGAQIIAQENTRLWLTTDVTWPWNGDTVQPLPAVARPNKTFFGRDE
                     LVVDGKRVQYGHIPHAPHTDGDMYVFFADANVLAVGGAMTAEGWQVIDWWTGGWIGGV
                     VGGLELLLSLTDAQTRIVTARGPVLTRADLEEQFQMYSTVYERLSRLLNSGRSPREAV
                     ESSVTVEFDAKMGNSDEFVTRAFESMWAYLSPDA"
     gene            1608..4010
                     /locus_tag="VFY27_09225"
     CDS             1608..4010
                     /locus_tag="VFY27_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEX5765736.1"
                     /translation="MAAVALTTGCEQFGFGSSPPIAQVGERWGMVDKYCTDCHNGLEL
                     AGNVAFDEFGPQDVAEKPELFERVVRKLRGRLMPPPTQQQPDDDELYAFVAAVERTLD
                     EAAAANPRSEVMGLHRLNRREYANAVRDLLALEIDPVGLLPQDEKVEGYDVIASALQV
                     SPSFIEQYVISARLVALQAVGRPNARAGSQTYNAGPGRQLTHVAGLPLGTRGGILAEH
                     MFPSDGEYEVNVADVIGGLWGSDMEFENTLVVTLDGKLVHQVVVGGEEDMKRFDQIHN
                     GALDAINVRLKNIRFKATAGPHKLGVAFRHRTFAESDDRIQMHVPGGGQDRVFRISSF
                     QVSGPFEPSGLSPTPSREKIFTCYPTRADEQQLCAEEIISSIGSRAFRRPLTETDVTE
                     LLQYYQDGLQVGGFEEGIRSALTGILASPYFLYRGERVPQNLSAGTQYKIDDLELASK
                     LSFFFWNTIPDQELLDLAAGGKLKNEAVLREQIKRMLTDPRAATLGSNFVYQWLDMQR
                     LDEVEPDRAVFPYASGSADPRKDFITELALFAQSIFDEDRSVVDLLTADHTYVNERLA
                     LHYGIRSVKGERFQRVKLEESPRWGLLGKGAVLMAAAYPNRTSPVLRGAFILKHISGA
                     PLPAPPAEVPLLVEQETTSATFVTVRAQMEAHRTDPVCASCHSVMDPLGFALENFDAV
                     GAWRDRDRFAAGTAPLDTSAQLPDGTEIQGPDDLREALVKRPEQFVQTFAEGLLTYAL
                     GRTLDYHDMPTVRKIVREAARDDYRFSSVVWAIVTSEPFQLRQVPVTNAAEVTAQNVS
                     GQ"
     gene            4083..5369
                     /locus_tag="VFY27_09230"
     CDS             4083..5369
                     /locus_tag="VFY27_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEX5765737.1"
                     /translation="MFISKKHLSRRTVLQGAGATIALPLLDAMNPAGVAWAATAAGQA
                     PHRMAFVGFPMGAILSKWSPEKAGKDFEISPILEPLEKYREHLTIVSGLRNKPAESPE
                     PHGLVERTWLSCVSPAQAGVLSPDAGVTADQMAVRHIGQETRLPSLELSTALRGAQLS
                     WRTPKQSLPQEPNPRAVFYRLFGQGDTDAERQAIVQETGSILDRVKGQAGRLQAGLGG
                     HDRASVSDYLDSVREIERRVQMSEAQDMGSLTLPDAPIGVPNDIDSHMKLMFDLMGLA
                     FQADLTRIITFSLDREASMRTYSNLGISEAFHPLSHHGNDAGKQARLVTIQNYHTKLF
                     AGFVGALAATKEGDGTVLDHSTILYGSNMSDSNRHNNDPLPAAVLGRAHGRIKGGQHL
                     RYPQDSRFADLLVTLFDRAQIPVESIGDSGGLLSEV"
     gene            5371..>6116
                     /locus_tag="VFY27_09235"
     CDS             5371..>6116
                     /locus_tag="VFY27_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5765738.1"
                     /translation="MKAHLAGLIAVLTSTAVIAADAALLDAVESGNRATALQLLEQGA
                     DVQVRGADGTTALMWAAYHGDVELARRLIRAGAEVNAANDFGASALSEAATIGSTPII
                     KALLDAGADANSSNPEGERPLMVVARTGNVEAAALLLKAGADVNHKEEWGGQSALMWA
                     AAQRQPEMVKLLVASGADVNARGVVRQWERKVIAEPRPKDMNRGGFTPLLYAAREGCI
                     DCAKHLLAGGADPDLPDPERVTALNLALTNL"
BASE COUNT         1143 a         1798 c         2036 g         1139 t
ORIGIN      
        1 cgctcaacta cctcaccaaa tggaagtcac agcaagttcc gggctcaccg gtggtggatt
       61 acaagggctc gggcgctgag gccggcctct acgattacct gccgtttaca acgttctcct
      121 accggaacga tcgtttgagc gtgggactca actggcgcta tctgccggcg atcgagcatt
      181 cgtcccttcc ggacaacccg acgtcgaccc agcagggggc gggctcgtac agctacttca
      241 gcctgaacgg cggctggcgc ttcaacgaga cgatgagact ccgggccggc atcgacaatc
      301 tgttcgacca agagccggaa atctacggcc gaacggacga gaacaacgcc ggcatcacca
      361 cgctcaccgg ccgttacgat ccgctgggtc gcaggggctt cgtagcgctg gaggtgaatt
      421 tctagcgtcg accctgaaat tgttaagggt tcgtcggcga atggcgtgcg atgacgcacg
      481 ccgttcgcca cgtgtcgtta ggaggttggt gtcgtgtcgt tgccttcccg tagaagcgtc
      541 ctgacggccg ccgtgggcgg cgccgcgggg gtctcgctcg gcctgttgcc aggcttccgc
      601 tctttcgtgg cgcaggccca atcgggcgct aaccggacgc tcgacctcgg cggcggcttg
      661 catgtgctga ccgtcgggca gaccaacgtc gtcgcgcgga cgggcgccga gggcgtcatg
      721 ctcatagacg gcggtgctgc tgctcagtcg gccgcgcttt cggcgctgat ctcggcgctt
      781 ccggcgaccg gaaaagttca cacgctgttc aatacgcact ggcatcccga gcagacgggg
      841 ttgaacgaag cgctcggcag agcgggtgcc caaatcatcg cgcaagagaa cacgaggttg
      901 tggctcacga ccgacgttac ctggccgtgg aacggcgata ccgttcagcc gctgccggcc
      961 gttgcgcgcc cgaacaagac gttcttcggt cgagacgagc tcgttgtgga cggcaagcgc
     1021 gttcagtacg ggcacatccc gcacgctcca cataccgacg gcgacatgta cgtcttcttc
     1081 gccgacgcga acgtgctggc cgtcggcggc gcgatgacgg cggaaggttg gcaagtcatc
     1141 gactggtgga cgggcggctg gatcgggggt gtcgtgggcg ggctcgagct gctgctctcg
     1201 ctgaccgacg cgcaaactcg aatcgtaacc gctcgtgggc cggttctgac ccgggcggac
     1261 ctcgaggaac agtttcagat gtacagcacg gtatatgagc gcctgtcgcg gctgttgaac
     1321 agcgggcgga gcccgcggga agctgtggag tcgagcgtga ccgtggaatt cgatgcgaaa
     1381 atgggaaatt ccgacgaatt cgtgacccgg gcgttcgaga gcatgtgggc gtacttatcg
     1441 cccgacgcct gagcaaaccc cggacggctc aataaacgtg aagtgctcgc tggagggctg
     1501 gtgttagata taacatgttt gggctccctc gtgcgcatcc tcgtgcgcat gacgtaaggc
     1561 caagtgagca acatatgtcg atgcgattga ctgcccagat tgggctggtg gccgcagtgg
     1621 cgctaactac cggctgcgag cagttcggtt tcgggtcgtc accgccgatc gctcaggtgg
     1681 gcgaacgctg ggggatggtc gacaaatatt gtaccgactg ccacaacggt ctcgagctag
     1741 ccggcaacgt cgcattcgat gagttcgggc cgcaggacgt cgccgagaag cccgagttgt
     1801 tcgagcgggt ggtgcgcaaa ttgcgcgggc gtctcatgcc tccgccgact cagcaacagc
     1861 ccgatgatga cgagttgtac gctttcgtgg cggccgtcga gcgaacgttg gatgaagcag
     1921 ctgctgcgaa tccgcgttcc gaggtcatgg gattgcatcg cctcaatcgc cgggaatatg
     1981 cgaacgcggt ccgcgatttg ttggccctcg agatcgatcc tgtggggctg ctgcctcagg
     2041 acgaaaaagt cgaagggtat gacgttatcg cgagcgccct acaggtgtcc ccgtcgttca
     2101 tcgagcagta tgtgatctcg gcgcgtctcg tcgcgttgca ggcggtcggc cgcccgaatg
     2161 cgcgagcggg cagccagacg tacaacgcgg ggccgggaag gcagctcacg catgtggcag
     2221 gtcttccgct cggcactcgc ggcggcatct tggccgaaca catgtttccg tccgatggtg
     2281 agtatgaagt caacgtcgcg gacgtgatcg gcggattgtg gggcagcgac atggagttcg
     2341 agaacactct agtcgttacg ctcgacggca agctcgtcca ccaggtcgtg gtgggcggcg
     2401 aagaggacat gaagcgcttc gatcagattc ataacggcgc gctggatgcc atcaacgtgc
     2461 gcctcaagaa catccgcttc aaagccacgg ccgggccgca caagctgggc gttgcctttc
     2521 ggcaccgaac gttcgcggaa tcggatgaca gaattcagat gcacgtgccg ggcggtggtc
     2581 aggatcgcgt gtttcgcatc agctccttcc aggttagcgg tcccttcgag ccctcggggc
     2641 tgagccccac gccgagtcga gagaagatct ttacctgcta tccgacgagg gccgatgagc
     2701 agcagctgtg cgccgaagag atcatctctt cgatcggctc gcgcgcgttt cgccgtccgc
     2761 ttacggagac ggacgtgacc gagctgttgc agtactacca ggacgggctt caggtcggcg
     2821 gattcgagga aggcattcgg agtgcgctca cgggtattct cgccagcccg tatttcttgt
     2881 accgcgggga gcgcgtacct cagaacttga gtgcgggcac gcagtacaag atcgatgatt
     2941 tggagctggc gtcgaagctg tccttctttt tctggaacac catccccgac caagagctgc
     3001 tggacttggc ggccggcggc aagctgaaaa acgaggccgt gctgcgcgag cagatcaagc
     3061 gcatgttgac ggacccgcgc gccgcgacgt tggggagtaa cttcgtctac cagtggctgg
     3121 atatgcagcg tttggacgaa gtggagcccg acagggcagt gttcccgtat gcctcgggaa
     3181 gcgccgaccc gcggaaggac ttcattacgg agcttgcgct gttcgcgcag agcatcttcg
     3241 acgaagaccg tagtgtggtc gacctgctca cagcggacca cacctacgtc aacgagcgcc
     3301 tagcgttgca ctacggtatc agaagcgtga aaggcgagcg ttttcagcgc gtcaagcttg
     3361 aggagtcgcc gcgttggggg ctgctcggaa aaggcgccgt gttgatggcg gccgcctatc
     3421 cgaaccgcac gtccccggtg ttgcgcggcg cgttcatcct caagcacatc agcggggcgc
     3481 cgctcccggc accgccggcc gaggtgccgc tcctcgtcga gcaggaaacg accagtgcga
     3541 ccttcgtaac ggttcgagcg cagatggaag cccaccgcac ggatcccgtc tgcgcgtcct
     3601 gccattcggt gatggatccg ctcggcttcg cgctggagaa tttcgatgcc gtaggcgcgt
     3661 ggcgtgatcg cgatcgcttt gcggcaggca ccgcgcctct cgacacctcg gctcagctgc
     3721 cagatggcac ggagattcag ggtccggacg atttgcgtga ggcgctggtg aagcgtcccg
     3781 agcagttcgt gcagactttt gcggaggggt tgctgaccta tgcgctggga cgcacgctgg
     3841 actaccacga tatgcctacc gttcgaaaga tcgtgcggga agcagcgcgt gacgactatc
     3901 ggttctcgtc ggtcgtgtgg gcgatcgtga ccagcgaacc gttccagctc aggcaagtac
     3961 cggtaacgaa tgcagcggaa gtgacagcgc agaacgtgtc gggccagtag cctaatctcg
     4021 ctgcgagttc cgagagttga aagatgattt gggattagtc gagtaaatcg aggagagagc
     4081 agatgttcat ttcgaagaag catctttcgc gccgaaccgt actgcaaggt gcgggggcta
     4141 ccatcgcgct gccgctcctc gatgccatga atccggcggg ggtcgcatgg gccgctaccg
     4201 cggcagggca agcgcctcac cgcatggcct ttgtcggctt cccgatgggc gcgatcttga
     4261 gcaaatggtc gccggaaaag gcggggaagg acttcgagat ctccccgatt ctggagccgt
     4321 tggagaaata ccgcgagcac ctgaccatcg tgtcgggact gcgcaataaa cccgccgaaa
     4381 gccccgagcc tcatgggctc gtcgagcgca cgtggctgag ttgcgtttcg ccagcgcagg
     4441 ccggtgtgct tagcccagat gcgggcgtta ccgccgacca gatggcagtg cgacatatcg
     4501 gtcaagaaac gcgcctgccg tcgctcgagc tttcaactgc ccttcgcggt gcgcagctgt
     4561 catggcggac gccgaagcag tcgttgccgc aggagccgaa cccacgcgcg gtcttctacc
     4621 gtctgttcgg acagggtgac acggacgcgg agcgtcaagc gatcgtccag gaaaccggca
     4681 gcattctaga ccgcgtcaag ggtcaagcgg gccgactgca agccggctta ggcggccacg
     4741 accgcgcatc cgtcagcgac tatctggatt ccgttcggga gatcgagcgg cgcgtgcaga
     4801 tgagcgaggc acaggacatg ggctcgctca cgcttcccga tgcgccgatc ggagtgccga
     4861 acgacatcga ttctcacatg aagctcatgt tcgacctgat gggtctcgcg tttcaggcgg
     4921 acttgacgcg catcatcacc ttctcgctgg atcgcgaggc gagcatgcgc acctacagca
     4981 atctcggcat ttcggaagcg tttcatcctc tgtcgcacca cggtaacgat gcgggcaagc
     5041 aggcgaggtt ggtcacgatc cagaactacc acaccaagct gttcgcaggc tttgtcggtg
     5101 cactagcggc caccaaggaa ggcgatggaa cggtgctcga tcattccacg atcctctacg
     5161 gcagcaacat gagcgacagc aaccggcaca ataacgatcc gctgccggcg gccgtgctgg
     5221 gtcgcgctca cggtcggatc aagggaggac agcatctgag gtacccgcag gattcgcgtt
     5281 tcgcggactt gctggtcacg ctgttcgacc gtgcgcagat tccagtggaa tcgatcgggg
     5341 acagcggcgg cttgctctcg gaggtctagt atgaaagccc atctggccgg tctcatcgct
     5401 gtgctgacga gcacggccgt tatcgccgcc gacgcggcgc tgctcgacgc ggtcgaatcc
     5461 ggcaatcggg caaccgccct gcagttgctg gagcagggag ccgacgtgca agtgcggggg
     5521 gcggacggca cgacggcctt gatgtgggcg gcatatcacg gtgacgtgga gttggcgcgg
     5581 cggctgatcc gcgccggtgc cgaagtcaac gcagccaacg atttcggagc ttctgctttg
     5641 tcggaggctg ccacgatcgg ctcgacaccc atcatcaaag cgttgctcga tgccggggcg
     5701 gatgcgaatt cctcgaaccc cgagggcgag cgacccctca tggtggttgc acggacgggc
     5761 aacgtagagg ctgccgcgct actgctgaag gccggggcgg acgtgaacca taaggaagag
     5821 tggggcggtc agtcggcgtt gatgtgggcc gcggctcagc gtcagccgga aatggtgaaa
     5881 ctcctcgtcg ccagtggcgc tgacgtcaac gcccggggcg tggtgcgcca gtgggaacgc
     5941 aaagtgattg cggagccgcg tcccaaggat atgaaccggg gcggtttcac gccgttgctc
     6001 tatgcggctc gcgagggttg catcgactgc gcgaagcatc tgctggccgg cggcgcggat
     6061 cccgatctgc cggaccccga acgcgtcacc gcgttgaatt tggcgctgac caatct
//
LOCUS       DASUOM010000447         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_534863,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000447 DASUOM010000000
VERSION     DASUOM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534863"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFY27_09240"
     CDS             complement(<1..792)
                     /locus_tag="VFY27_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765739.1"
                     /translation="MKHDPRNCRNRGLLCAVFLLLLIACPPLSRSQAILQSDIVDPRA
                     FGYTVGDKIRREMVLTLNSGYVLDEESLPEAGRLDRWLEVAAPEIRAEAEGDATRYRI
                     VLTYQIFNAPLALETVTVPQQDIRILTNGEEEQTAFTTLIPALRVTVAPVTSAVDAGR
                     LSDASLQADRAPAAIPVEGRQRRIAWVSFALLVMLLYAAWRGGIAVFLSRGKLPFTRA
                     LRELRKLQPARGTPAPYASGLKIVHDAINSTAGRATFAHNLDDFLA"
     gene            complement(835..1746)
                     /locus_tag="VFY27_09245"
     CDS             complement(835..1746)
                     /locus_tag="VFY27_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765740.1"
                     /translation="MNAGLAPGEVRPGRTGGSAGTATSQSVERFHYRLPGRARGAEPG
                     AHPGSQRGGGMEFKGHASLLTAQDPRRFDVAASLRDPFNQVIVRVYTQRSAIPVRVIA
                     DVSASMGFTGRCSKIDLLADFTESLALSASRAGDPVSFMGCDSTIREDLLLPLTRSRA
                     AGQLLAARLRELKPSGRNALGLFAAVEQTPPSRSLVFLVSDFYFPLSMLVELLGGLAL
                     HEVVPVVLRDSAEGQAPRYGIARIVDPETGVERMLLLRPSLARRLQDCVAARGEALKR
                     CCARFGLKPLFIEDRFEADAVTRYFYG"
     gene            complement(1743..>2143)
                     /locus_tag="VFY27_09250"
     CDS             complement(1743..>2143)
                     /locus_tag="VFY27_09250"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765741.1"
                     /translation="RLSEGRVPFDQLNDVAERVQDHIQTSAVLQRYALDLCRATSDPH
                     AYGVRMDEVDVPQLVQSGVSPRGTSMLLRAARVAAWLNGRDSVTPEDIHEVFRVTVGH
                     RVFLRPVYELRRSELIGQFLDRVLDRVAAP"
BASE COUNT          389 a          711 c          688 g          355 t
ORIGIN      
        1 ggcgaggaag tcatcgaggt tgtgcgcaaa agtggcgcgc ccggcagtgc tgttgatagc
       61 gtcgtggacg atcttgaggc cggaggcata tggtgctggc gtacccctcg ccggctgcaa
      121 ctttcgcagc tcgcgcaagg cgcgggtgaa aggcaacttt ccacgtgaca ggaaaactgc
      181 gatcccgccc cgccatgccg cgtaaagcag catcacgagc aatgcgaagc ttacccaggc
      241 gatgcgcctt tgccgccctt cgaccggtat cgccgcaggc gcgcggtctg cctgcagcga
      301 agcatccgac aaccttcccg catcgacggc tgatgtcacg ggcgccaccg tgacacgcaa
      361 ggccgggatc agcgtcgtaa acgccgtttg ttcctcctcg ccgttggtga ggatacggat
      421 gtcctgctgc ggaacggtga cggtttccag cgcaaggggt gcgttgaata tctggtacgt
      481 caggacgatc cggtaccggg tggcgtcacc ttcggcttcg gcgcggattt ccggtgcagc
      541 gacctcgagc caccggtcca gccgacctgc ttcaggcagg ctttcttcgt ccagcacata
      601 gcccgagttc agggtcagga ccatctccct gcgtattttg tcgcccacgg tgtagccgaa
      661 tgcacgcggg tcgacaatgt cgctctgaag aatcgcctgc gaccgggaga gcggcgggca
      721 ggcaatgagc agcagcagga acacagcgca aagcaggccg cggttacggc aattccgggg
      781 atcgtgcttc attggctcgg tatgcttttc gtacacctgc ggatgccgat ggcctcagcc
      841 gtagaaatac cgggtcaccg cgtctgcctc gaaccgatct tcaatgaaga gcggcttcaa
      901 tccgaagcgc gcgcagcagc gcttcagcgc ctcgccccgt gcggcgacac agtcctgcag
      961 gcgccgcgcg agtgacggcc gcagcaacag catgcgctcg accccggttt ccggatcgac
     1021 gatgcgcgcg atgccgtaac gaggcgcctg gccctccgcg ctgtcgcgca agacgaccgg
     1081 caccacttcg tgcagcgcca gcccgcccag cagttcgacc agcatcgaca gcggaaagta
     1141 aaagtccgat acgagaaaca ccagcgagcg cgagggcggc gtctgctcga ccgccgcaaa
     1201 gaggccgagg gcgttcctgc cggagggctt cagctctcgc aagcgtgctg ccagcaactg
     1261 ccctgccgcg cggcttctcg tgagtggcag cagcaggtcc tcgcggatgg tgctgtcgca
     1321 gcccatgaat gaaacgggat cgccggcccg ggaggcggaa agcgccagcg attcggtgaa
     1381 gtccgccagg agatcgatct tactgcagcg gccggtgaag cccatcgacg cggagacgtc
     1441 cgcaattacc cgaacgggaa tcgcactgcg ctgcgtgtag accctgacaa tcacctggtt
     1501 gaacgggtcc cgcaggctcg cggcgacgtc gaagcggcgc ggatcctgcg ccgtcagaag
     1561 gctggcatgg cccttgaatt ccataccccc gccgcgctgg ctgccgggat gggcgcccgg
     1621 ctcagcgccc cgggcgcgcc ccggcagcct gtagtgaaat ctctcgacgc tctggctcgt
     1681 tgccgttccg gcggatccgc cagtcctgcc gggccgcacc tcccccggcg ccagaccggc
     1741 attcacggcg ctgcaacccg atccagcacc cggtccagga attgcccgat cagctccgag
     1801 cgccgcagtt cgtagaccgg cctcaggaac acgcggtgac ctacggtcac gcggaatacc
     1861 tcgtggatat cctccggggt gacggaatcg cggccattga gccaggcggc caccctcgcc
     1921 gcccggagaa gcatgctggt tccccgcggg cttaccccgc tctgtacgag ctgcggaacg
     1981 tcaacctcgt ccatgcggac gccgtacgcg tgcggatccg acgtggcgcg acaaaggtcc
     2041 agtgcgtaac gctgcaggac agccgaggtt tggatatggt cctgcacgcg ttcggcaacg
     2101 tcgttcagct ggtcgaaagg cactctccct tcggatagcc gct
//
LOCUS       DASUOM010000448         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1436231,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000448 DASUOM010000000
VERSION     DASUOM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436231"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFY27_09255"
     CDS             <1..720
                     /locus_tag="VFY27_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765742.1"
                     /translation="NFPSAFPGGPPATADAGDEAEEDPDAPAEAAPPEESEDADGAPG
                     SETTPAHIAESTEAASVIVVADVDLLSDRMWVQVQNFFGQQIANAFADNGTFVINALE
                     SLSGSNDLIDVRSRASYSRPFTVVADLRARAEAQFQETEQSLQQELEETERRLSELQS
                     SRDDSGDILMTEEQQAEIDRFIDRRAEIRQELRAVQRGLDRDIERLGTVLKIVNIGLV
                     PLLLTIFVLFAVWRRSRRGRT"
     gene            717..1928
                     /locus_tag="VFY27_09260"
     CDS             717..1928
                     /locus_tag="VFY27_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX5765743.1"
                     /translation="MSSRNVRILAVVVLALFGVLFALNSTDRSDSQTGAQMLFPEIKS
                     RLNDLNVVTITDADGTITLEREAEDDSAAARWISSAHDGYPADTGKLRQLLLAIADAR
                     KLEQKTADPELYERLGVADPREDGGNGVLVSARGNDAAVSVILGDTAQQEFRYARLPD
                     EEQSWLIDQNPDLPGDSTGWLLPEIVDIAATRIQSVIIRHADGEEVRIGKENADAANF
                     ELENIPEDEELRYPSVANGIAGVLGNLALQDVRKGDLAGEDASTAEFRTFDGLELLVR
                     IGSRAGEANAGDGDDGANTDEEEHWITLEASADDAVGDSPDADEGEEPVEDADSSAGE
                     APQSAEANGEDGAPGGQEASEGKRDPADEAAEINDRVSGWSYRIAGYKADQLTRRLAD
                     LLSEEEDPPAE"
     gene            2015..2641
                     /locus_tag="VFY27_09265"
     CDS             2015..2641
                     /locus_tag="VFY27_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5765744.1"
                     /translation="MSVRDVEAEDINPDNIKVVVFDLGGVLLQLRDPVETFEVQMPIT
                     LFLERWLKSPSVREFERGAADAETFAKAIVQEMELAMDWQEFLRRFDSWPERIFPETT
                     ALLDSLPGGVAKVLLSNTNAAHWGRDDIGPSLSGRFDTTFLSFETGLLKPDRDAFLQV
                     IAAYGMPAHAFLFFDDNPLNVAAAAETGMQAVLCKGAAEALRVTRNLQ"
     gene            complement(2633..>3175)
                     /locus_tag="VFY27_09270"
     CDS             complement(2633..>3175)
                     /locus_tag="VFY27_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5765745.1"
                     /translation="YKQFFHDRSPRAKDLQIATYEHEFAYDWQDDECKLWFSEDGLPG
                     YAWYVPKARGYVNVGLGGKADAMKSKTLRLQDHWDRFVRNLEKRGLVTGANYDPSGYS
                     YYLRGSVDTVRLDNIFIVGDSVGLASKDLGEGIGPAVRSAILAAEAIVDNAEYSLRNV
                     DGYSVPGFLQQRILRRAASL"
BASE COUNT          633 a          953 c         1049 g          540 t
ORIGIN      
        1 aacttcccca gcgcgttccc gggcggcccg ccggcaactg ccgatgccgg ggacgaagcg
       61 gaggaggacc cggacgcacc agccgaagcc gctccgccgg aagaaagcga ggacgcggac
      121 ggagcgcccg ggagcgaaac gacgccggcg catattgccg agtcgacgga agctgcaagc
      181 gtcattgtcg ttgcagacgt ggatttgctc agcgaccgca tgtgggtaca ggtccagaac
      241 ttctttggcc agcaaatcgc aaatgccttc gccgacaacg gcaccttcgt gatcaatgcg
      301 ctcgagagcc tctccggcag caacgacctc atcgatgtga gaagccgcgc cagttactcg
      361 cggccgttca cggtcgtcgc agacctgcgg gcgcgggcgg aagcgcaatt ccaggaaacc
      421 gagcagagcc tgcagcagga actggaggaa accgagcggc gcctgtcgga actgcagtcg
      481 tcgcgcgatg acagcggcga catcctgatg acggaggagc agcaggcaga gatcgaccgc
      541 tttatcgacc gccgcgccga aatccgccag gaactgcgcg ccgtgcaacg tggcctcgac
      601 cgcgatatcg aacggctggg cacggtactg aagatcgtca acatcggcct ggtcccgttg
      661 ctgctcacga tcttcgtcct gttcgcggtg tggcgccgaa gcaggagggg caggacgtga
      721 gctcacgcaa cgtcaggatt cttgccgtcg tagtgctggc cctgttcggg gttctgttcg
      781 cgctgaactc gacggaccgg agcgattcgc aaacgggcgc gcaaatgctg ttcccggaga
      841 tcaagtcgcg gctgaacgac ctcaacgtcg tcacgatcac ggatgccgat ggaacgatca
      901 ccctggagcg tgaagcggaa gacgactctg cagccgcccg ctggatttcg tccgcgcatg
      961 acgggtatcc cgccgatacg ggaaagctcc ggcagctgtt gctggcaata gcggatgcgc
     1021 ggaagctcga acagaagacg gcggatccgg agctatatga gcggcttggt gttgccgacc
     1081 cgcgggaaga tggcgggaat ggcgtgctgg tttcagcgag aggtaacgat gcggcggtct
     1141 ccgtgatact cggggacacg gctcagcagg agttccgcta cgcgcggctg ccggatgaag
     1201 agcagagctg gcttatcgac cagaaccccg acctgcccgg cgacagcacc ggctggttgc
     1261 tgccggagat cgtggacata gccgcaaccc gaatccagtc cgtcatcatc cggcatgcgg
     1321 atggcgaaga agttcgcatt ggcaaggaga acgcggacgc agctaacttc gagctcgaga
     1381 acattcccga ggacgaagag cttcgctacc cgtcggttgc caatggaatc gccggcgtac
     1441 tgggcaacct ggcgctgcag gacgtgcgta agggagatct ggccggtgaa gacgcatcga
     1501 ctgcggagtt caggaccttc gatgggctcg agctactggt gcggattggc agccgggccg
     1561 gcgaagccaa cgccggggac ggtgatgacg gtgccaacac cgatgaggag gagcactgga
     1621 taacactgga ggcttccgca gacgatgcag tcggcgattc gccggacgcg gacgaaggcg
     1681 aggagccggt cgaagatgca gactcttccg caggggaagc accgcaatcc gccgaggcca
     1741 atggcgagga cggtgcaccg ggaggccagg aagcgagtga aggcaagcgg gatccggccg
     1801 atgaagcagc cgaaatcaat gaccgcgtca gcggctggtc gtacaggatc gccggctaca
     1861 aagccgacca gctgactcgc cgccttgcgg atctcctgag cgaagaggaa gacccgcccg
     1921 ctgaatgaag cgagcggcaa cccgcgcctg cgtcctcaac ggagaagcca ggactgccgg
     1981 tgcagttcac ttccggcgga gcacagccag ctgcatgagc gtacgtgacg tcgaggccga
     2041 agacatcaac cccgacaaca tcaaggtcgt cgttttcgat ctgggcggtg tcctgctgca
     2101 gctccgcgac ccggtggaaa ccttcgaggt tcagatgccc atcacgctgt tcctggagcg
     2161 ctggctaaag tcgccgtcgg tgcgcgagtt cgaacgcggc gcagcggatg ccgagacctt
     2221 tgcgaaagcc atcgtgcagg agatggaact ggcgatggac tggcaggagt tcctgcgcag
     2281 gtttgattca tggccggagc gcatttttcc cgagacgacg gcgctactcg acagtttgcc
     2341 cgggggagtt gcaaaggtgc ttctgtcgaa tacgaatgca gcgcactggg gccgcgacga
     2401 tatcgggcca tcactctccg ggcggttcga cacaacgttc ctgtccttcg agacagggct
     2461 tttaaagccg gaccgcgacg cgtttctcca ggtcatcgca gcgtacggca tgcctgcgca
     2521 cgcctttctc ttcttcgacg ataacccctt gaacgttgcg gcggccgccg aaacgggcat
     2581 gcaggcggtg ctgtgcaagg gagcggcgga ggctctgcgg gtaacgcgga atctacagtg
     2641 acgccgccct tcgcaggatc cgctgttgca ggaagcccgg gacgctgtat ccgtcgacgt
     2701 tgcgcaggct gtattccgcg ttatcgacta ttgcctccgc ggcaaggatc gcgctgcgca
     2761 cggcaggccc gattccctcg cccaggtctt ttgaagccag gcccacggag tcgccgacga
     2821 tgaaaatgtt gtcgagccgc accgtgtcca cgctgccgcg caggtaataa ctgtagccgc
     2881 tggggtcgta gttcgcgccg gttaccagcc cgcgcttctc caggttgcgt acgaaccggt
     2941 cccagtgatc ctggaggcgc agtgttttcg acttcatcgc gtccgccttg cctccaagcc
     3001 ctacgttcac gtatcccctg gccttcggca cgtaccaggc atatccggga aggccgtcct
     3061 cggagaacca gagcttgcac tcgtcgtctt gccagtcgta agcgaactcg tgttcgtagg
     3121 tcgctatctg caggtccttc gcgcgcgggc tgcgatcgtg gaagaactgc ttgta
//
LOCUS       DASUOM010000449         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1622294,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000449 DASUOM010000000
VERSION     DASUOM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622294"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..901)
                     /locus_tag="VFY27_09275"
     CDS             complement(116..901)
                     /locus_tag="VFY27_09275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5765746.1"
                     /translation="MTAKDNSWVLVTGASSGFGEEFARQYAAQGRPLVLVARRLEKLQ
                     ALARELHERHNVDVIVEQVDLSAITAVVELHKRLRERGVTIDVLINNAGHGWQGPFLE
                     EPLDGAMAMLDLDVASMTAVTRLFAEDMRARGHGQILMVASLLAYQGVQNFAIYSAAK
                     AYVLRLAEALHRELKRDGIIVTALCPGMSDTGFAAAAQQKITPALKLLMMQPAPVVRA
                     GIRALQAGRTSVVPGWGNKAFVFFTWATPRWLHQAITARVMGA"
     gene            complement(981..1268)
                     /locus_tag="VFY27_09280"
     CDS             complement(981..1268)
                     /locus_tag="VFY27_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765747.1"
                     /translation="MNIKTLRSLAAALMVTSLPLTAIAESLGEHPAVLAARTWNSRDV
                     DPNTLGLIPNTFVFLHPAGPVWVDRSPTVGEDDVVRDPPPRMVSAENVSSQ"
     gene            1378..2289
                     /locus_tag="VFY27_09285"
     CDS             1378..2289
                     /locus_tag="VFY27_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5765748.1"
                     /translation="MDRLMALQVFARVVELGGFTKAANSLQLSKTTVSDLVQGLEKHL
                     GVQLLQRTTRRVTVTPDGAAFYERSAHILADLDEAEASVMQARVAPKGRLRVDMPSAL
                     ARLFILPQLPTFLARYPELRLELGMGLRPVHLVEEGIDCVVRIGMQPDSSLIARRVGT
                     MTFVCCASPAYLREHGTPRRPEELSTHRCVNYLSNRTGRVIDWQFSRGREKVDLTLDG
                     VLAVNDFDAYVAAGLMSFGIVKVGSYVARPYLESGQLTQVLADWTAEQLPIWVMYPQS
                     RHLSAKVRTFVDWVSELIQQDPIFQTR"
BASE COUNT          599 a          880 c          879 g          590 t
ORIGIN      
        1 tcactggtgt gctgccttcg aatcgaagag cacggtccct tgcccgccct ctacttcgcc
       61 ggaccggaga tggccgacag cagatcggtg cggcggccgc atcgccgtga tcacttcagg
      121 cgcccatgac gcgagcggta atggcttggt gcagccagcg cggtgtggcc caggtgaaga
      181 acacgaaagc cttgttgccc caacctggca cgacgctggt gcgtccggct tgcagcgcac
      241 gaatccccgc gcgcacgacg ggcgcaggct gcatcatcag cagcttcaac gcgggcgtga
      301 tcttctgttg cgcagctgcc gcaaatccgg tgtccgacat gcccggacag agtgcggtca
      361 cgataatgcc atcgcgcttg agttcacgat gaagcgcctc ggcgaggcga agcacataag
      421 ccttggcagc ggaatagatc gcaaagttct gcacgccctg ataagcgagc agactggcaa
      481 ccataagaat ttgtccatgc ccccgagccc gcatgtcctc agcgaagagg cgtgtcaccg
      541 cggtcatgct ggcgacatcc agatccagca tcgccatcgc tccatccagc ggctcctcca
      601 ggaaaggccc ctgccatcca tggccagcgt tgttgatcag gacgtcgatc gttacaccgc
      661 gctcgcgcag tcgcttgtgc agttcgacga ccgccgtgat cgcggaaaga tcgacctgct
      721 ccacgatcac atcgacgtta tgacgctcgt gcagctctct cgccagcgct tgcagcttct
      781 ccagccgccg tgccacaagg accagcggcc gaccttgtgc tgcgtattgc cgggcgaatt
      841 cctcaccgaa accactcgat gcgcccgtga caagaaccca cgaattgtct ttcgctgtca
      901 ttctgtccac ctggtttgcc gtccgcacac agaatcgggc accggcggca gttctttccc
      961 tgtaccggca gcttctcgcg tcactgactc gacacatttt ccgccgacac catccgcggt
     1021 ggcggatcgc gcacaacgtc gtcttccccg accgtcgggg atcggtcgac ccagacggga
     1081 cccgccggat gcaggaagac gaacgtattg ggaatgagtc ccaatgtatt ggggtcgaca
     1141 tcgcggctat tccacgtccg cgcggcgagc acggcaggat gttcaccaag ggattccgcg
     1201 attgcggtga gcggtagcga agtgaccatt aaggctgcgg ctagcgagcg tagggttttg
     1261 atgttcattt gcatgactcc ttgaagcgtt ttcattgatc aggtgcctgc actataattg
     1321 gccgccagaa agggataaat cggctaaatc cggaatcact gtccgggagt gcggacaatg
     1381 gatcgactga tggccttgca ggtattcgct cgcgtcgtcg aactgggcgg cttcactaaa
     1441 gcggcaaatt ccctgcaatt gtcgaagacg accgtgagcg acctcgtgca agggctcgag
     1501 aagcaccttg gtgtgcaact gctccagcgc accacgcggc gtgtgaccgt cactccggac
     1561 ggtgccgcgt tctacgagcg ctccgcgcac atccttgcgg atctcgacga ggcggaggcg
     1621 tccgtcatgc aggcacgcgt cgcgccgaag gggagactgc gcgtcgatat gcccagtgcg
     1681 ctcgcgaggc ttttcatcct tccgcaactg ccgacgttcc ttgcgcgtta tcccgaactg
     1741 cgattggaat tggggatggg gcttcgcccg gtacatctgg tggaggaggg catcgattgc
     1801 gtcgtgcgca tcggcatgca gccggactcg agcctgatcg cacgacgagt cggtacgatg
     1861 accttcgtct gctgtgcgag ccctgcgtac ctacgggagc atggaacccc gcgcaggccc
     1921 gaggagctat ccacccatcg gtgcgtcaac tacctttcga atcgcaccgg acgagtcatc
     1981 gactggcaat tctcgcgggg ccgagagaag gtggacctca cactcgacgg tgtcctcgcg
     2041 gtgaacgatt tcgacgcgta tgtggcagcg ggtctcatga gttttggaat cgtgaaggtt
     2101 ggcagctacg tggcgcggcc gtacctggag tcggggcagc tcacgcaggt gctcgctgac
     2161 tggacagcgg aacagttacc catctgggta atgtatccgc aaagccgtca cctctcggca
     2221 aaggtgcgca ccttcgtcga ctgggtcagc gagctgattc agcaggatcc catcttccag
     2281 acgcgctgaa tatcgcggca ccgcgaaggc gttggctgga acgcgttgtt gggcaggagg
     2341 ttctcccgac gaccttcaaa aagtcagtcc atcttcgatg accgttcggt tgctccattt
     2401 tcaagcacct tggctatcgc gtgatggtat tccagttacg cctcgtaact ggtacgccga
     2461 agagccggtc caacgtgccg agatagctga tggcgttcat gcgccgtcgg tgtagcccga
     2521 cgacatacat ccgggtaccg cctgcgcgtt gctggcgtca acttcgagga tcgtcaggat
     2581 ttgctgggac acaagtccga acggatcacg acgcattact cagcgccgga tatcgcaaga
     2641 ctggtggaag cggcggagaa agtgtgtgaa cgacgaccga ataccatatt acggatcgca
     2701 gctcacacga atctcgcaca atcgctcgca caaaagggca gcagtcgggt ggaacacgca
     2761 cgaggagttg agggataagg acttattggt cggggtgaaa ggttttgaac ctccggcccc
     2821 tgcctcccga agacattcag gcgttttaag taaatattat ttatagagaa actggagacg
     2881 cccgttgaca agttgacaca acaagtcata acgattccgg accgattccg gcaattctcc
     2941 ggcacttg
//
LOCUS       DASUOM010000450         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1659244,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000450 DASUOM010000000
VERSION     DASUOM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659244"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFY27_09290"
     CDS             complement(<1..577)
                     /locus_tag="VFY27_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5765749.1"
                     /translation="MADNAARQLAPQLPDLNAYWMPFTGNRAFKSSPRMISRAEGMHC
                     YTHDGKKLLDAVAGLWCCNAGHCHPKIVEAIQKQAETLDYSMAFQLGHPKVIELANRL
                     TEITPSGIDYAFFVNSGSEAVDTALKMALAYHRLRGEGERTRLIGRARGYHGVGFGGI
                     SVGGISPNRKMFGAQLPGVDHLPHTHNLEHNA"
     gene            complement(609..1889)
                     /locus_tag="VFY27_09295"
     CDS             complement(609..1889)
                     /locus_tag="VFY27_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5765750.1"
                     /translation="MLAASDHANSYYSATLTERTDYPVLTGEVRADVAVVGAGFTGVS
                     AAITLAERGYDVVVVEANRVGWGASGRNGGQLIDGFVDLEKVESRLDAGALDIAYRMG
                     LECRDLVLERIGKYGIECDLKFGFVDLALRQRDLDYFLSEIERKARLDYPHRMQFVPK
                     EELPSIIGSTRYIGALVNHGNGHLHPLNLCIGEARAAAGLGVRFFERSPVTRIRHGSA
                     PAVETANGRVVAKKVVLAGNAYLGRTEPKLYGAVIPAGSFMIATEQLDPELARELLPQ
                     DMAACDQRVALDYFRLSADKRLLFGGLCNYSGRNPKDISASLRPNMLRVFPQLEDARI
                     EYEWGGYIAISMKRIPQLGRIDGNSYYAQGYSGHGLAPSHLAGKVIADAIGGDFERFD
                     VFERIGHWKLPGGKWFANPALALGMLYYRLKELI"
     gene            2021..2563
                     /locus_tag="VFY27_09300"
     CDS             2021..2563
                     /locus_tag="VFY27_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5765751.1"
                     /translation="MEDVASRLKRLRVRHGLSQRKLAALSGVSNATISLIEHGLTDPS
                     LGLLKKVLDAMGVSFAGFFSEGGVEEKFFYSSDELSEIGSGLISYRQVGGDLLDSKLQ
                     ILHERYRPGADTGQSMLSHEAEEGGIVLQGRLEVTVGSQVRTLSPGDAYRFNSRLSHR
                     FRNTGSEDCIVVSACTPPSF"
     gene            2659..>2845
                     /locus_tag="VFY27_09305"
     CDS             2659..>2845
                     /locus_tag="VFY27_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765752.1"
                     /translation="MAEIKRTEGSHSVKGNTAGHASTRRASAITTTAIRSPCKCHFYF
                     DLAAYKQTISIATVAPAN"
BASE COUNT          571 a          895 c          840 g          539 t
ORIGIN      
        1 gggcgttgtg ctccagattg tgcgtgtgcg gcaggtggtc gacgcccggc aactgcgcgc
       61 cgaacatttt ccggttcggc gaaatgccgc ctacggagat accgccgaaa ccgacgccgt
      121 gatagccgcg cgcacgaccg atgagccgcg ttcgctcgcc ttcgcctcgc agccggtggt
      181 aggccagcgc catcttcaat gccgtgtcga cggcttccga acccgagttg acgaagaacg
      241 cgtagtcgat cccgctcggg gtgatttcgg tcaggcggtt tgccagttcg atcaccttcg
      301 gatgaccaag ctggaacgcc atcgaataat caagcgtttc cgcctgcttc tggatcgcct
      361 ccacaatttt cgggtgacag tgcccggcat tgcagcacca gaggccagcc actgcgtcca
      421 ggagcttttt tccgtcgtgc gtatagcagt gcatcccttc ggcgcgggaa atcatccgcg
      481 ggctgctctt gaacgcgcga ttgccggtaa atggcatcca gtaggcgttg aggtcaggca
      541 attggggtgc cagttgccgt gctgcgttgt cggccatcag ttcatctccg aaacttgcga
      601 tacgtccgtc aaatcaattc tttcaaacga taatagagca tgccgagcgc gagtgccggg
      661 ttggcaaacc acttgccgcc gggcaatttc cagtgcccta tccgctcgaa tacgtcgaac
      721 ctttcgaaat ccccgccaat ggcatcggcg atgactttgc ctgccaggtg tgaaggcgcc
      781 agcccatgcc cggaatagcc ttgcgcgtag tagctgttgc cgtcgatgcg accgagctga
      841 ggaattcgct tcatgctgat ggcaatatac ccgccccact cgtattcgat ccgcgcatcc
      901 tcgagttgcg ggaacactct cagcatgttc gggcgcaggc tcgcgctgat gtccttcggg
      961 ttgcggccgg aatagttgca caggccgccg aacaggagcc gcttgtcggc ggacaggcga
     1021 aagtaatcga gggccacgcg ctggtcgcag gccgccatgt cctgcggcaa aagttccctc
     1081 gccagctcgg ggtcgagctg ctcggtcgcg atcatgaagc tgccggccgg aattactgct
     1141 ccatacagct tcggttccgt ccgacccagg taggcgttgc ctgccagcac gactttcttt
     1201 gctaccactc ggccgttcgc cgtctccacg gccggagcac tgccgtgacg aatgcgcgtc
     1261 accggcgagc gctcgaaaaa tctcacaccc agcccggcgg cagcacgcgc ctcgccgatg
     1321 cacaggttca gcggatgcaa atggccgttg ccgtggttta ccagcgcacc gatgtagcgc
     1381 gtggagccga taatggaagg aagctcctcc ttcggcacga actgcatccg gtgcggatag
     1441 tctaggcgcg ccttgcgttc gatctcgctt aagaaatagt cgaggtcacg ctgcctcagg
     1501 gcgagatcga caaagccgaa cttaaggtcg cactcgatgc cgtatttccc gatccgttcg
     1561 agcacgagat cgcggcattc aagtcccata cggtaagcga tgtcgagggc accggcatcg
     1621 aggcgcgatt cgaccttctc gagatcgacg aagccgtcga tcaactgccc gccgttcctg
     1681 ccggacgcac cccagcccac gcggtttgcc tcgacgacca cgacgtcgta gccgcgctcg
     1741 gcaagagtta tggccgcgga gacgccggta aacccggcgc cgaccacggc gacgtctgca
     1801 cgcacctccc cggtgagcac cgggtaatcg gtgcgttcag tgagggtcgc cgaataatag
     1861 gaattggcgt ggtcggaggc agcaagcatg ggcgaacagg gtactccagc ccctgggggt
     1921 taacaatatt taaacgagtg attataactc gctgaattac tggagacgaa cagctttttt
     1981 gtttagcata ttcaacaatt caacaagacc ggggaaagct atggaggatg tcgcctcgag
     2041 actcaaacgg ctgcgggttc gccacggcct ttcgcaacga aaactggctg cactgtccgg
     2101 cgtgtccaac gccacgatct cgctgatcga gcacggcctc acggacccgt cgctcggctt
     2161 gctgaaaaag gtactggatg ccatgggtgt gtcgttcgcc gggttcttct cggaaggcgg
     2221 cgttgaggaa aaattcttct attcgtccga tgaactgagc gaaatcggca gcggcctgat
     2281 ctcctacagg caggtaggcg gcgatctcct ggacagcaaa ttgcagatcc tgcacgagcg
     2341 ctaccggccc ggcgccgaca ccggccagtc gatgctgagc cacgaagccg aggaaggcgg
     2401 catcgtgctt cagggccgcc tcgaggtcac cgtcggcagc caggtccgca ccctgtcccc
     2461 cggcgacgcc taccgcttca acagccgcct ctcgcaccgc ttccgcaaca ccggcagcga
     2521 agactgcatc gtcgtctccg cctgcacccc gccgagcttc tagcgccggt gcgaacgcag
     2581 gacaccggca acaatcagac gacggcaggg ccagaacgaa gggtccgacg acaggaggct
     2641 tggttggcgg tgttgccttt ggcggaaata aagcggaccg aagggagcca ttccgtcaaa
     2701 ggcaacaccg ccggccatgc ctctacgcgg cgagcaagtg cgataacgac gaccgctata
     2761 aggtcacctt gcaaatgcca cttctacttc gacctcgccg cgtacaaaca gaccatctcc
     2821 atcgccaccg tagcccccgc caacg
//
LOCUS       DASUOM010000451         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_52129,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000451 DASUOM010000000
VERSION     DASUOM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52129"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFY27_09310"
     CDS             complement(<1..466)
                     /locus_tag="VFY27_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353701.1"
                     /note="involved in methylation of ribosomal protein L3;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5765753.1"
                     /translation="MTRHGSSRPTVGEFIERAAERFDAAGLVFGHGTDNALDEAAWLV
                     FARLQLDHNDAEFAYRRLLTSQEVAELEQLIRRRIHERIPVAYLVQEAWFAGLPFFVD
                     ERVLIPRSPIAELVVNKFRPWLRDVRRALDIGTGSGCIAIAVARAFPGARVDA"
     gene            571..1182
                     /locus_tag="VFY27_09315"
     CDS             571..1182
                     /locus_tag="VFY27_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5765754.1"
                     /translation="MAALAAVALGVALLFLWNVDDNGPEPQGAGPRFATVLPEPLPLP
                     DFALRDQEGEPFTLDSLRGRTSLLFFGFTHCPDICPATLQQLSLARKQIAATTPGTAL
                     PAIVLISVDPERDTPEKLEKYVSAFGEDIAGLTGTRESLLPLTSAMGIYFERQPLEGG
                     DYTVSHSTAVLVVDADANLRALFSAPHKTEYFAHDIPLLMAAQ"
     gene            1179..1634
                     /locus_tag="VFY27_09320"
     CDS             1179..1634
                     /locus_tag="VFY27_09320"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5765755.1"
                     /translation="MSHRARLAGAILVLLGACSAEESPPLGVSEVTAYAALPGQPAGV
                     AYFSLQNSTPGAVTLRQVSSPEYSSVEMHMTLMDDGVARMTPLDLLTVAGRSAVIFAP
                     GGPHLMLMEPIEGLATGDEVTLVFHFDYDGDLDDDGPGLLTVRTPLLSR"
     gene            1651..2526
                     /gene="asd"
                     /locus_tag="VFY27_09325"
     CDS             1651..2526
                     /gene="asd"
                     /locus_tag="VFY27_09325"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX5765756.1"
                     /translation="MMRLKSQAFLLMQRLLPQHLLMALVGRVAGIRIRVIKDFLIRTF
                     VSFYNVDTAECARAVPEGFRDFNDFFTRELVEGARPVDGAPASLVSPVDGILSAAGRI
                     DGSSLLQAKGIHYTLEDLLATDLDQAARFREGSFATMYLAPYNYHRVHSPLSGRLIAA
                     RYVPGHLYSVNQATVDLLPRLFTRNERLICHFDTAAGPVALIFVGALNVGSVSTPWTG
                     RIRVRKKGVVDDLALAGAGISDDIEKGQMIGWFNMGSTVIVLLPPGTCSWREHLVNGT
                     RLRMGEAIGTLNLEH"
     gene            2516..>2974
                     /locus_tag="VFY27_09330"
     CDS             2516..>2974
                     /locus_tag="VFY27_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5765757.1"
                     /translation="MSTERWRPSADVATARRRARMMAQARSFFAERNVLETDTPLLSP
                     TTVSDPHVESVQAVLASRPGKRYFLQTSPEYFMKRLLCSGYPDIYQVCKVLREGEAGR
                     RHSPEFTMVEWYRHSFNLARMMQETAAFISALVDPQHLAAPPEFIDYRRAF"
BASE COUNT          521 a          968 c          947 g          538 t
ORIGIN      
        1 cggcgtctac gcgcgccccg ggaaatgccc gcgcgactgc aatggcgatg cagccgctgc
       61 ccgtcccgat atcgagagca cgccgcacat ctcgaagcca cgggcggaac ttgttgacga
      121 ccagttccgc gatgggggat cggggtatga gcacgcgttc gtccacgaag aacggcagtc
      181 cggcgaacca cgcttcctgc acgaggtagg cgaccgggat acgctcgtgg atgcgccggc
      241 gaatcaactg ctcaagctcc gcaacctctt ggctcgtcag tagccggcgg tacgcaaatt
      301 ccgcatcgtt atgatctagc tgtaacctgg cgaacaccag ccacgcggcc tcgtcgagcg
      361 cattatccgt gccgtgcccg aaaacgagac cggctgcgtc gaagcgttcg gcggcccgtt
      421 cgatgaattc tcccactgtg gggcgggagg agccgtgcct ggtcatatgc cggaggttct
      481 cgttcaactt tgaaagcggg ttaaaatcag cggttcatgg cgagcccagg acgaatttcg
      541 aacccaggac aaattccacg acacctgtgg atggctgcgc tcgccgctgt cgcccttggg
      601 gttgcgcttc tgttcctgtg gaacgtcgac gacaacggac cggagccgca gggagcgggg
      661 ccccggtttg ccaccgtgct gccggaacca ctgccgctgc cggacttcgc cctgcgtgac
      721 caggagggcg agccgtttac cctggacagt ctgcgcgggc gaacaagctt gctgtttttc
      781 ggcttcactc actgcccgga catctgcccg gcgacgttgc agcaattgtc gctcgcgcgc
      841 aagcagatcg ctgcaacgac gccggggact gcgttgcccg caatcgtact aataagtgtg
      901 gacccggaac gggacacgcc ggagaagctc gagaagtacg tgtcggcgtt cggcgaggat
      961 atcgcagggc ttaccggcac tcgcgagtcg ctcttgcccc ttacttcggc aatgggcata
     1021 tatttcgagc gccagccgct cgaaggcggc gactacaccg tcagccactc gacggcggtg
     1081 ctcgtcgtcg acgccgatgc aaatctccgg gccctgttca gcgctcccca caagacggaa
     1141 tacttcgctc atgatatccc gctcctgatg gcggcacagt gagccaccgt gcacggctcg
     1201 cgggggcgat cctggtcctg ctcggcgcct gcagtgcgga agagtcgccg ccgctggggg
     1261 tgtccgaggt gacggcgtac gccgccctgc cgggccagcc ggcgggcgtg gcctactttt
     1321 cgttgcagaa cagcacccct ggggcagtga ccctgcgaca ggtcagcagc cccgaatact
     1381 cgagcgtcga gatgcacatg acgctgatgg atgacggggt cgcgcgaatg actccgctcg
     1441 acctgctgac cgttgccggg cggtcggcgg tcattttcgc cccgggcggt ccgcacctga
     1501 tgctcatgga gccgatcgaa ggccttgcga ccggcgacga ggtgacgctc gtttttcact
     1561 ttgactacga cggcgacctg gacgacgatg gtccggggct cctcaccgtg cgtaccccgc
     1621 tgctgtcccg ctgacatgca gcgcagcttc atgatgcgat tgaagtcgca ggcgttcctg
     1681 ctgatgcagc gcctgctgcc gcaacacctg ctgatggcgc tggtcggcag ggtggcaggc
     1741 atccgcattc gcgtcatcaa ggatttcctg atccgcacct tcgtgagctt ctataacgtc
     1801 gacacggcgg agtgcgctcg cgcggtgccg gaaggcttcc gggacttcaa cgacttcttc
     1861 acccgcgagc tggtcgaagg cgcaaggcct gtcgacggcg cacccgcgtc gctcgtgtcg
     1921 ccggtggacg gcatcctgag cgccgccgga cggatcgatg gcagctcgct cctgcaggcc
     1981 aagggaattc actacactct cgaggacctg ctggcgacgg acctggacca ggccgcgcgg
     2041 ttccgcgagg gcagtttcgc cacgatgtac ctggccccct acaactacca tcgtgtccac
     2101 agccccctga gcggccggct cattgccgcg cgctacgtac cgggccacct ctacagcgtg
     2161 aaccaggcga ctgtcgactt gctgccgcgc ctgttcacgc gaaacgagcg gctaatttgc
     2221 catttcgaca cggccgcagg ccctgtcgcg ctcatcttcg tcggcgcgct caatgtcggc
     2281 agtgtgagca caccctggac cgggcgcatc cgggtgcgga agaaaggcgt ggttgacgat
     2341 cttgcgcttg caggcgccgg catttccgac gatatcgaga agggccagat gatcggctgg
     2401 ttcaatatgg gttcgactgt catcgtgctc ctgccacccg gcacctgctc atggcgggaa
     2461 cacctggtta acggcacccg ccttcgcatg ggcgaggcga tcggcacgtt gaaccttgag
     2521 cactgagcgg tggcgtccct cggcggacgt ggcgactgcg agacggcggg cgcggatgat
     2581 ggcgcaggcg cgaagcttct tcgccgagag gaacgtcctc gaaacagaca cgccgctcct
     2641 ctctcccacc acggtgagcg acccgcacgt cgaaagcgtg caggcggtgc tcgccagccg
     2701 gccaggcaaa cgttacttcc tgcagacgtc accggagtat ttcatgaagc ggctgctctg
     2761 cagcggctat ccggacatct accaggtatg caaggtgctc cgcgaaggag aagccgggcg
     2821 ccggcacagc cctgagttca cgatggtgga atggtatcga cacagcttca acctggcccg
     2881 gatgatgcag gaaaccgccg ccttcatctc ggcgctggtc gatccgcagc acctggcggc
     2941 accgccggaa ttcatcgact accgccgtgc cttc
//
LOCUS       DASUOM010000452         6544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_535270,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000452 DASUOM010000000
VERSION     DASUOM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535270"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..735)
                     /locus_tag="VFY27_09335"
     CDS             complement(112..735)
                     /locus_tag="VFY27_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5765758.1"
                     /translation="MSGPRRSSGSWRDRQERDPFVRKARVEGWRSRAVFKLEQIAAKE
                     PILFPGMVCVDLGSAPGGWSQYVATRLNNRVRIVAMDLLPMDSLPCVEFLQADFSTDE
                     GLALLLRQLDGTPVDLVMSDLAPNISGNRAIDQPRAMHLAELALDTARKVLRYKGNFV
                     CKLFQGEGTDDFVALARESFEKVRIMKPKASRDGSSEVYLVARNYRL"
     gene            763..1062
                     /gene="yhbY"
                     /locus_tag="VFY27_09340"
     CDS             763..1062
                     /gene="yhbY"
                     /locus_tag="VFY27_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEX5765759.1"
                     /translation="MNLTEAQKRYLRGLGHKLKPVITVGGAGVSDSLVKEFDSTIDHH
                     ELVKVRFRVTDRQQRDEMINQLCKKGNAALVSRTGHTAILYRRTHDAPKIQLPPG"
     gene            complement(1166..1642)
                     /gene="greA"
                     /locus_tag="VFY27_09345"
     CDS             complement(1166..1642)
                     /gene="greA"
                     /locus_tag="VFY27_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5765760.1"
                     /translation="MNKVPMTVRGAQLLREELRKLKSEDRPRIIQAIAEARAHGDLKE
                     NAEYHAAKDQQGFVEARIKEIEAKLSNTQVIDVTTVDAKGKVVFGATVELLDEQTGKE
                     IVYRIVGEDEADIKDGLISFTSPIARALIGKNEGDDVSFSAPGGEKRYEVIQVRYE"
     gene            complement(1639..4863)
                     /gene="carB"
                     /locus_tag="VFY27_09350"
     CDS             complement(1639..4863)
                     /gene="carB"
                     /locus_tag="VFY27_09350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5765761.1"
                     /translation="MPKRTDIESVLIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPETADAVYIEPVNWAAVERVIAKERPDALLPTMGGQTALNCALDL
                     VREGVLARYNVELIAASHAAIDMAEDREQFKEAMTEIGLEVARAEIAHTLEDAFEKQK
                     AIGFPVIVRPSFTLGGTGGGIAYNREEFEEIVAHGLEMSPTTEVLLEESVIGWKEYEM
                     EVVRDKNDNCIIVCSIENVDPMGVHTGDSITVAPAQTLTDKEYQRMRDASIEVLRKIG
                     IETGGSNVQFAVNPEDGRMLVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LQNDITGGATPASFEPTIDYVVTKIPRFTFEKFPGSNTRLTTQMKSVGEVMAIGRNFQ
                     ESLQKALRGLETDIDGLEERCTGARGVLDRGGLSQELRHPGPERILYVADAMRSGFSV
                     GEINELTGIDPWFLVQIEDLVSEEMALQKEGMASLDYTRMRRLKRKGFSDARLARVLK
                     QKEMLVRQRRLKLKLRPVYKRVDTCAAEFPTSTAYLYSTYEDECEADPSDRPKIVILG
                     GGPNRIGQGIEFDYCCVHASFALKKAGYETIMVNCNPETVSTDFDTSDRLYFEPLTFE
                     DVMEIIEKENPQGVIVQYGGQTPLKLARGLEAAGAPIIGTSPDSIDLAEDRERFQKLV
                     DKLGLRQPPNRTARSHEQALKMAAEVGYPLVVRPSYVLGGRAMEIVHGEDELSSYMAA
                     AINVSNESPVLLDRFLDVAIEVDVDAICDGERVLIGGIMEHIEQAGVHSGDSGCSLPP
                     FSLKPAVLEILVEQVTRLAKGLNVIGLMNTQFAIQNGVVYLLEVNPRASRTVPFVSKS
                     IGIPLASIAARVMVGESLEQQGFVNQRIPPYYSVKESVFPFLRFAGADPILGPEMKST
                     GEVMGTGRTFGEAYAKAQLASGVVLPTQGVALLSVRDRDKRSAVELAQILAARGFDIV
                     ATHGTANELAKAGVSCRRANKVREGRPHIVDMIKNGEISLIVNTTEGKQAIYESRSIR
                     AEAVRKNVTYYTTIGAAVATCKALEHLEDSDVNRLQDLHKEVAA"
     gene            complement(4955..6118)
                     /gene="carA"
                     /locus_tag="VFY27_09355"
     CDS             complement(4955..6118)
                     /gene="carA"
                     /locus_tag="VFY27_09355"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5765762.1"
                     /translation="MLSTPAVLALQDGTIFHGVSVGDDGKTIGEVVFNTAMTGYQEIL
                     TDPSYCRQIVTLTYPHIGNTGTNSEDMESGKIHASGLIIRDSSMVSSSWRSERSFGEF
                     LRQGKTVAIADIDTRKLTRLLREKGAQSGCIMTGDVDPQVAVQHARKFPGLKGMDLAK
                     FVSTDRPYQWCYGTEFGRRNRIMSRRVAPYHVVAYDFGIKRNSLRILSDLDCRMTVVP
                     AMTPPEEVMALRPDGVFLSNGPGDPEPCSYAIKATREFLKVSMPLFGICLGHQLLALA
                     TGARTVKMKFGHHGANHPVKDLQTGRVMITCQNHGFAVDESTLPDNVLVTHRSLFDGT
                     LQGIALKDRPALSFQGHPEASPGPHDLRPLFDRFIADMDRAKRNAPAAAVGAF"
     gene            complement(6341..>6544)
                     /locus_tag="VFY27_09360"
     CDS             complement(6341..>6544)
                     /locus_tag="VFY27_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765763.1"
                     /translation="TRRGEVAGEHTVIFRSEAESLELTHRVSDRRVFAQGALRAAGWL
                     VGQPPGFYRMSDMMGGSQERKRN"
BASE COUNT         1199 a         2081 c         1883 g         1381 t
ORIGIN      
        1 tcgaagcggc ctcgcttagc catacattgc tcgctgcgct cggcttcaaa gtctccttgg
       61 cgggacgttc ttggcaggcg cgttagactg cgtctaagag tttagacagt actatagtcg
      121 atagtttctc gctacaaggt acacctcact gctgccatcc cgtgaggctt tgggcttcat
      181 gattcgtacc ttttcgaaag attcccgggc gagcgcgacg aaatcgtcag taccctcgcc
      241 ctgaaacagt ttacaaacaa aattgccttt atatcgcagt acctttctcg ccgtatcgag
      301 ggccagctca gcgagatgca tggctcgcgg ctggtcgatg gcacggttgc cgctgatatt
      361 gggagcgagg tcgctcatta caaggtcgac cggagtcccg tccagttgcc gcagcagcag
      421 ggcaagcccc tcgtcggtgc taaagtccgc ttgtaagaac tcgacgcaag gcaaggaatc
      481 cattggtaaa agatccattg caacgatgcg gaccctgttg ttcagccggg tcgctacgta
      541 ctggctccag ccccccggtg cggagcctag atccacgcac accattccgg gaaacaggat
      601 cggctccttg gcagcgatct gttccagctt gaagacggcg cgcgagcgcc agccctcgac
      661 gcgcgccttc cgcacgaacg gatcgcgctc ctggcgatct cgccacgagc cgctgctgcg
      721 cctgggaccg ctcatccgga gccactgtta caattcaatc gcatgaacct gacggaagca
      781 caaaagcgtt atcttcgtgg attgggtcac aaactcaagc ccgtgatcac cgtcggcggc
      841 gccggcgttt cggactccct ggtaaaggag ttcgactcca cgatcgacca ccatgaactg
      901 gtaaaggtca ggttccgcgt cacggaccgg caacagcgcg acgagatgat caaccagctg
      961 tgcaagaagg ggaacgcggc cctcgtgtcc cggacggggc atacggcgat cctctatcgg
     1021 cgcactcacg acgctccaaa aatccagctg ccgccggggt gaccgggctc gcggccttga
     1081 ctcggacacc tgtaggagcc cgccgtgcgg gcgagcgcaa tcggccatgg cgaactcgcc
     1141 cgcatggcgg gctcctacat cgcccttact cgtagcgaac ctggatgacc tcgtaacgct
     1201 tttcgccgcc gggagctgaa aagctgacgt cgtcgccttc gttcttgccg atcagcgccc
     1261 gggcgatcgg cgacgtgaac gagatcagcc cgtccttgat gtcggcttcg tcctcgccga
     1321 cgatccggta aacgatctcc ttgccggtct gttcgtcgag aagctcgacg gtcgcaccga
     1381 agacgacctt tcccttcgca tcgaccgtcg tgacatcgat gacctgggtg tttgacagct
     1441 tcgcctcgat ttccttgatg cgcgcttcca cgaagccctg ctggtctttc gcggcatggt
     1501 attccgcatt ctccttcaga tcaccgtgcg cccgggcttc cgcgatcgcc tggatgatcc
     1561 gcggacgatc ctcggatttc agcttccgca attcctcgcg caggagctgg gcaccgcgca
     1621 ccgtcatcgg taccttgttc acgcggccac ctccttgtgc aggtcctgca gacgattgac
     1681 gtcgctgtcc tccaggtgtt cgagcgcctt gcaggttgca accgccgcgc cgatcgtcgt
     1741 gtagtacgtg acgttcttgc gcactgcttc cgcccggatc gaccgggatt cgtaaatagc
     1801 ctgtttgccc tcggttgtat tcacaatcaa cgaaatctca ccgtttttga tcatgtcgac
     1861 gatgtgaggt cgaccttccc gtaccttgtt cgcccggcgg caggataccc ccgccttcgc
     1921 cagctcgttt gccgtaccgt gcgttgctac gatatcgaag ccccgcgctg ccaggatctg
     1981 cgcaagctcc acggcacttc gcttgtcccg gtccctgacg ctaaggagcg ccacgccctg
     2041 tgtcggcaaa acgacgccgg acgccagttg cgccttggca taggcttcac cgaatgtgcg
     2101 ccctgtcccc atgacctcgc cggtcgattt catctccggc ccgaggatgg gatcggcgcc
     2161 ggcgaaacga aggaacggaa agaccgactc cttgaccgaa tagtacggtg ggatgcgctg
     2221 gtttacgaag ccctgttgct cgagactttc gcccaccata acacgggcgg caatgcttgc
     2281 cagcggtatc ccgatcgatt tggagacgaa cggcaccgta cgcgatgccc ggggattgac
     2341 ctccagcagg tagaccacgc cattctggat ggcgaactgg gtgttcatca gcccgatgac
     2401 gttcaatccc ttcgccagcc gcgtcacctg ctcgaccagg atttccagca ctgcaggctt
     2461 caggctgaat ggcggtaacg agcagccgga atccccggag tgcacgcctg cctgctcgat
     2521 gtgttccatg atgccgccga tcagcacgcg ttcaccatcg caaattgcgt ccacgtcgac
     2581 ttcgatggcg acgtcgagaa agcggtccag gagcacgggg ctttcgttcg agacgttgat
     2641 ggcggcggcc atgtaggacg acaattcatc ctcgccgtgc acaatctcca tggctcgccc
     2701 gccgaggaca taggagggtc gtaccaccag cgggtaccct acctccgccg ccatcttcag
     2761 cgcctgctcg tggcttctgg cagtgcggtt cggaggctgc ctgaggccga gcttgtcgac
     2821 cagcttctgg aagcgttccc ggtcttctgc caggtctatc gaatcgggcg atgtgccgat
     2881 gatgggtgct ccggccgctt ccaggccgcg cgccagcttg agaggagtct ggccgccata
     2941 ctgcacgatg acgccctgcg gattctcttt ctcgataatt tccatgacgt cttcgaacgt
     3001 cagcggttcg aagtacaatc ggtcggatgt gtcgaagtcg gtcgagaccg tctcggggtt
     3061 gcaattaacc atgatcgtct cgtaaccggc tttcttcagc gcgaacgacg catgcacgca
     3121 gcaatagtcg aactctatcc cttggccgat acggttggga cctccgccca ggatcacgat
     3181 ctttggccgg tcgctgggat ctgcttcgca ctcgtcttcg taagtggaat agaggtacgc
     3241 cgtcgatgtc gggaattccg cggcgcaggt gtcgacacgc ttgtaaaccg gccgcagctt
     3301 gagctttaga cggcgctgcc ggaccagcat ctccttctgc ttcaggacac gtgcgagacg
     3361 ggcatccgag aaccccttgc gtttcagtcg ccgcatccgc gtgtaatcaa ggctggccat
     3421 gccttccttc tgcagcgcca tctcctcgct cacgaggtct tcaatctgta cgaggaacca
     3481 cggatcgatg ccggtaagtt cgttgatttc gccgacgctg aagccgctgc gcatggcatc
     3541 cgccacgtac agtatccgct ccggacccgg gtgccgaagt tcctggctga ggccgccccg
     3601 atcaaggacg cctcgcgcgc cggtgcacct ctcctcgagc ccgtcgatat cggtttccag
     3661 tccccgcagc gccttctgca gcgactcctg gaagttgcgc ccgatcgcca tgacttcgcc
     3721 caccgacttc atctgcgtcg tcaggcgggt attggatccc gggaacttct cgaacgtaaa
     3781 ccggggtatc ttcgtgacga cgtagtcgat cgtcggttcg aacgaagccg gcgtggcgcc
     3841 gccggtaata tcattctgca gctcgtccag cgtatagccc accgcaagct tcgccgcgat
     3901 cttcgcaatg ggaaagccgg tagccttcga cgccagcgcc gatgacctcg agacgcgcgg
     3961 gttcatttcg atcaccagca tccgaccgtc ttccggattg acggcgaact gaacgttcga
     4021 gccgccagtc tcgatgccaa ttttccgcag cacttcgatc gatgcgtcgc gcatgcgctg
     4081 gtattccttg tccgtcagcg tctgcgccgg ggcaaccgtt atggaatccc cggtatgaac
     4141 gcccatcggg tcgacgtttt caatggagca gacaatgatg cagttgtcgt tcttgtcccg
     4201 cacgacttcc atttcatact ctttccaccc gatgaccgat tcctccagca atacctcggt
     4261 cgtgggtgac atctccagtc cgtgcgcgac gatctcctcg aactcttcgc ggttgtacgc
     4321 aatgccgcca ccggtgccac cgagggtgaa cgaaggccgg acgatgaccg gaaaacctat
     4381 tgccttctgc ttttcgaacg cgtcctccag ggtatgcgcg atctctgccc tcgccacctc
     4441 gaggccgatc tccgtcattg cctccttgaa ctgctcacgg tcctccgcca tgtcgatggc
     4501 ggcgtgagag gccgctatca gttcgacgtt gtagcgcgcg agcacccctt cccgaacgag
     4561 atccagcgcg cagttgagcg cggtttgccc acccatcgtc ggcaacaggg cgtccggccg
     4621 ttccttcgcg atcacccgtt ccaccgcggc ccagttcacc ggctcgatgt agacggcatc
     4681 cgccgtctcc gggtccgtca tgatggtcgc agggtttgaa ttgacgagga tgacccggta
     4741 gccctcctct cgcagggcct tgcaggcctg tgcgccggag tagtcgaact cgcaggcctg
     4801 cccgataacg atcgggcccg ccccgatgat caggacgctt tctatgtcgg tgcgttttgg
     4861 catggctttg ctgtgcggaa cttgaagaga ccgcgcgacg gaggaaaaga aacctgctga
     4921 agaaaaaggt ataggtaact gtttaatata aagatcagaa cgctcccact gcggctgccg
     4981 gtgcgttacg cttggcgcgg tccatgtcgg cgatgaatcg gtcgaacagc ggacggaggt
     5041 cgtgtggtcc tggacttgcc tccgggtgcc cctggaagct gagcgccggc ctgtctttga
     5101 gcgcaatccc ctgcagagtc ccgtcgaaca gcgaccggtg cgtcaccagc acattgtcgg
     5161 gcagcgtcga ttcgtcgacg gcgaagccat ggttctggca ggtgatcatg acccggcccg
     5221 tctgcaggtc cttcacggga tgatttgcgc cgtgatgacc gaacttcatc ttcactgtgc
     5281 gggcgccggt cgccagcgcg aggagctggt gtcccaaaca gattccgaac agcggcatcg
     5341 aaaccttcag gaactcccgc gttgccttga tcgcgtaaga gcagggctca ggatctcccg
     5401 gaccgttgga caggaacacg ccgtccgggc gcaacgccat cacttcttcc ggaggcgtca
     5461 tcgccggtac taccgtcatg cggcagtcga ggtcggacag aatgcgcagg ctgttccgct
     5521 tgatgccgaa atcgtatgcg acgacgtgat agggggctac gcgccggctc atgatgcgat
     5581 tgcgcctgcc gaactccgtg ccgtaacacc actggtacgg gcgatccgtc gagacgaact
     5641 tggcgaggtc catgccctta agcccgggga acttgcgtgc atgctggacc gcgacctggg
     5701 gatccacatc gccagtcatg atgcagccgg attgggcgcc cttctcccgc agcagccggg
     5761 tcagcttgcg cgtatcgata tccgcaatgg caacggtctt gccctggcgc agaaactcgc
     5821 caaagctgcg ctcggagcgc cagctgctgg agaccataga gctgtcgcgg atgatgagtc
     5881 ctgacgcgtg gatcttgccg gactccatgt cttcggagtt ggtgccggtg ttgccgatgt
     5941 gcgggtaggt cagcgtgact atttgccggc aataggaagg atcggtcaga atttcctggt
     6001 agccggtcat tgccgtattg aataccacct cacctattgt ctttccgtcg tctccgacgg
     6061 aaacaccgtg aaagatcgtt ccgtcctgca acgccagaac agcgggagta ctcaacagca
     6121 atcctcagct atcaacactt tatttcgacg attacccagg cgatcctcgt ttcggctatt
     6181 cgtttgagac acatgcgttc gacgcacagg ttcagcccgc cagatgcaca aaagcggaag
     6241 actttagcct tccgctcgag gtcgcgccgg tcctgcacga ttacggtatt ttactgaacg
     6301 ccgagcagtc cttccaccgt caggcaaagt ttattccgga ttagtttcgc ttgcgttcct
     6361 gagagccgcc catcatgtcg ctcatccggt agaaccccgg cggctgtccc accagccagc
     6421 ctgccgcccg cagcgctccc tgcgcgaaca cgcggcggtc ggacaccctg tgcgtcagct
     6481 ccagagactc ggcttcggag cggaagatga ccgtgtgctc cccggcgacc tcgccgcggc
     6541 gcgt
//
LOCUS       DASUOM010000453         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_412542,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000453 DASUOM010000000
VERSION     DASUOM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412542"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFY27_09365"
     CDS             <1..312
                     /locus_tag="VFY27_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX5765764.1"
                     /translation="LHGRYGPYITDKSKNARVPKDRDPATLTLGECRELLAAAPQRGR
                     RGKKKAAGNGAAASETANKASTEKKAGKKAKKKKKTAGKKTAKKSSKKAGLKDADNRP
                     A"
     gene            338..895
                     /locus_tag="VFY27_09370"
     CDS             338..895
                     /locus_tag="VFY27_09370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5765765.1"
                     /translation="MALSIPLRKAARILNEGGVIAYPTEGVFGLGCLPLLEDAVQRIL
                     GIKGRDPSLGLILIASSPSQLEPYALLPADEPELAGSLEQPVTWILPAKPATPLWIRG
                     RHPGVAVRICAHPVAAALCDAAGSALVSTSANVAGQPPARNSIVLRRRFGHLVDYIVP
                     GDCGPAKGASEIRDYASGTVLRPPV"
     gene            917..1840
                     /gene="hemF"
                     /locus_tag="VFY27_09375"
     CDS             917..1840
                     /gene="hemF"
                     /locus_tag="VFY27_09375"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5765766.1"
                     /translation="MNESDSAISSVGDYLQDLQARIVAALENADGRKTFMSDRWKREA
                     GGGGTSRVLVDGGVFEQAGVGFSHVFGDTLPESATRLRPELAGRRFQALGVSLVLHPK
                     NPHVPTTHANFRFFLAGDDDAAPVWWFGGGFDLTPFYPVHEDVLHWHRQAKDLCMPFG
                     DSLYDRYKKWCDDYFFLKHRGETRGVGGIFFDDLNEFGFQRSFDFLRAAGDRFLPAYL
                     PIVEARKSVSFGDREREFQLYRRGRYVEFNLLYDRGTLFGLQSGGRTESILMSLPPRV
                     RWEYNWQPESGSPEARLVDYLRPRDWLHEGG"
     gene            1981..>4361
                     /locus_tag="VFY27_09380"
     CDS             1981..>4361
                     /locus_tag="VFY27_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5765767.1"
                     /translation="MFLSRLILVSCCLASAAAWAQADSRLRDGGLAYGIRLAGSDDPG
                     GAQKVYIVQLKSPAAAENFAASYPRQSGKVSTTLKGPPQAFSKSAAGVESYAQRLSQE
                     QDKVLARVGAEKIYSYRYSLNGFAARMTPAEAHKLEHQPEVLRVWEDEVRPLGTSYSP
                     EFLGLFEEHVGLRGTPGLDGEGVVIAIIDSGITPAHPALADTREANRPRACRSLWAEN
                     TFLGKWLCRRYDKLEDTLVFDPPADWNGICQEGPEFPVDSCNNKLIGARWFSDGAEAG
                     GPIDEGEIFSARDVDGHGTHTATTAAGNRVGASIFGTFLGRVEGIAPRARVAAYKACW
                     LRPGDLRASCNTSDLANAIDAAVADGVHIINYSVGSSLLTTTAPDDVALLAATKAGVL
                     AIVAAGNEGPNFNTIGSPAGGPWVITSAASSRDGEHSVEALEVTAPSSVAGRYAVKEA
                     GFTPPLIDEGPLEGRLVLVDDDDDELEDGSAGTTFDGCEPLVNDSELSGNIAFIQRGG
                     CDFDVKIAHADEAGAIAAVVFSIAGNPIVMTGVADLSDIPALMIGQADGNLLLDEIDA
                     GLAVEVVLDKGLFLAEEDTGNVMGAFSARGPGPSEDILKPDVTAPGINILAGFTPDAA
                     NSVTGENFAFLTGTSMAAPHVAGVAALLRQAHPDWSPAAIKSALLTTAHQDVNQQDGV
                     TAAIPFDFGSGHIDPNPANNPGLVYDATADDYDAFACGTFSPAVDEARCDQLAAAGFS
                     FGPEDMNQPSIALSRLTGTQTVTRRVTNVSVETGSYTAEIEAPPGISVLVAPST"
BASE COUNT          829 a         1382 c         1414 g          736 t
ORIGIN      
        1 ttgcacggcc gctatgggcc ctacatcacg gacaagtcga aaaacgcacg ggtgcccaag
       61 gatcgggatc cggctacgct gaccctgggc gaatgccggg aactgctggc ggcggcgccg
      121 cagcgcggaa ggaggggcaa gaagaaagcg gcgggtaacg gcgcagctgc gagcgaaacg
      181 gcgaacaagg caagcacgga gaagaaggcg gggaaaaaag cgaaaaagaa gaagaaaacg
      241 gcaggcaaaa agactgcgaa aaaaagcagc aagaaagcag gtttgaagga cgcggataac
      301 cgtccagcct gagcagatcg ccgcaggaga cccggttttg gcgctgtcga tccccctccg
      361 aaaagctgcc cgcatcctga acgaaggcgg cgtcatcgcg tacccgaccg aaggagtgtt
      421 cgggctcggc tgcctgccgc tgctcgaaga tgcagtgcaa cgtatcctgg gcatcaaggg
      481 acgcgaccct tccctggggc tgatcctgat tgcatcttcg ccttcgcagc tcgagcctta
      541 cgccctcctg ccggcggacg aaccggagct cgccggcagc ctcgagcagc cggtgacgtg
      601 gatactgcct gcaaagcccg ctacgccgct gtggatacgc ggccggcacc ccggggtggc
      661 cgtgaggatc tgtgcacatc ccgtcgcagc cgcgttgtgc gatgcggcgg ggtccgcgct
      721 ggtatccacc agcgcgaacg tcgccgggca accaccggcg cgaaacagca tcgtcctgcg
      781 ccgccggttc ggccacctag tagactatat cgttcccgga gactgcggcc ccgcgaaggg
      841 cgcttcggaa attcgagact atgcgagtgg cacagtcctg cgtccgccag tttgacacga
      901 ggccgccaaa gcaagaatga acgaaagcga cagcgcgatt tccagtgtcg gggattacct
      961 gcaggatctg caggcacgca ttgttgccgc gctcgagaac gcagacggca ggaagacttt
     1021 catgtcggac cgctggaagc gcgaggcggg gggcggcggc acgagccggg tactggtaga
     1081 cggcggggtc ttcgaacagg ccggcgtcgg attctcgcac gtgttcggcg acacgttacc
     1141 ggaaagcgcc acccgcttgc gacccgagct cgccgggcgc cgcttccagg ctctcggcgt
     1201 atcgctggta ctgcacccga agaacccgca tgttccgacg acgcatgcca acttccggtt
     1261 cttcctggcc ggcgacgacg atgcagcccc ggtgtggtgg ttcggcggcg gcttcgacct
     1321 tacccctttt tatcccgtgc acgaggacgt gctccactgg cacaggcagg cgaaggacct
     1381 ctgcatgcca ttcggcgatt ctctttacga tcgatacaag aagtggtgcg acgactattt
     1441 tttcctgaag caccggggcg aaacccgcgg ggtgggcggc atttttttcg acgacctgaa
     1501 cgagttcggc ttccagcgct cgttcgactt cctccgcgct gcgggggatc gtttccttcc
     1561 ggcctatctt cccatcgtgg aagcgcgcaa gtcggtttcc ttcggtgacc gggagcggga
     1621 attccagctg tatcgccgcg ggcgttacgt cgagttcaac ctgctgtacg accgcggcac
     1681 gctgttcggg ctgcagtcgg ggggccggac cgaatcgatc ctgatgtccc tgccgccacg
     1741 ggtacgctgg gagtacaact ggcagccgga gagcggttcc cccgaagcac gtcttgtgga
     1801 ctatctccgt ccacgggact ggcttcacga gggcggctga cataagtggc gtggcgcgag
     1861 ccgtatatgc taagttcttt gcaaaaccgc gatttacacg gtgcttaaag acgacgactg
     1921 aacaataacg accgaactta gcaggggagc gtccggcagg cgggtgccgg gtattttttt
     1981 gtgttcttat caagactcat cttagtgtcc tgctgcctcg ccagcgcggc cgcatgggcg
     2041 caggcagaca gccgcttgcg cgatggcggt ctcgcctacg gcatccggct ggccggctct
     2101 gacgaccccg gcggtgccca aaaggtctac atcgtccagc tgaaatcacc cgccgcggcc
     2161 gagaacttcg cagccagcta cccacgccag tccggaaagg tctccacaac cctgaagggc
     2221 ccgccgcaag ctttcagcaa gagtgccgcc ggtgtggaga gttacgcgca gcgcctttca
     2281 caagagcagg acaaggtcct ggctcgcgtg ggtgcggaga aaatctacag ctaccggtac
     2341 agcctgaacg gtttcgctgc tcgcatgact cccgccgagg cacacaagct cgagcaccag
     2401 ccggaagtcc tgcgtgtctg ggaggacgaa gtgcggcccc tcggcacgag ttacagcccg
     2461 gaatttcttg gcctgttcga agagcacgtg ggactccgtg gaactcccgg tctggatggc
     2521 gagggcgtcg tgatcgcgat catcgacagc ggcattacgc ctgcgcatcc cgcgctcgcc
     2581 gacaccaggg aggccaatcg tccgcgtgcc tgccggagtc tctgggcaga gaacacgttc
     2641 ctcggcaaat ggctttgccg gcgctacgac aagctcgaag atacgctcgt gttcgatccg
     2701 ccggcggact ggaacggcat ctgccaggaa ggaccggagt tccccgtcga cagctgtaac
     2761 aacaagctca tcggcgcgcg ctggttttca gacggcgcgg aagcgggcgg cccgatagac
     2821 gaaggcgaga tcttctccgc ccgggacgtc gacgggcacg ggacccatac tgcaacgacc
     2881 gccgccggca accgcgtggg ggcttcgata ttcggcacgt tcctcgggcg cgtcgaaggc
     2941 atagcgcccc gagcgcgcgt cgccgcctac aaggcctgct ggctgcgtcc cggcgacctg
     3001 cgcgccagct gcaatacctc cgacctggcg aacgccattg atgctgccgt tgccgatggc
     3061 gtgcacatca tcaattattc cgtcggcagc tctttgctga cgaccacggc gccagatgac
     3121 gttgccttgc ttgcggcaac gaaagcaggc gtcctcgcca tcgtcgccgc gggcaacgaa
     3181 gggccgaact tcaacacgat tggttcaccg gccggcggac cgtgggtgat cacgtcggcg
     3241 gcctcgagcc gcgacggcga gcattccgtt gaagcactgg aagtaacggc accctcctcc
     3301 gtcgcgggcc gctatgccgt gaaagaagcc ggtttcacgc cgccgctcat cgacgagggc
     3361 ccgctggaag gccgcctggt gctggtcgac gatgacgacg atgagctgga ggatggcagc
     3421 gcaggcacta cgttcgacgg ctgcgagccg ctggtcaatg acagtgaact ctccgggaac
     3481 atcgccttca tacagcgcgg cggctgcgat ttcgacgtca agatcgccca cgcggacgaa
     3541 gctggggcaa ttgccgccgt ggtcttcagc atcgccggca acccgattgt catgaccggc
     3601 gttgcggacc tctccgacat tccggcgctg atgatcgggc aggcggacgg caacctgctg
     3661 ctcgacgaga tcgacgcggg gctcgcggtg gaggtcgtgc tggacaaggg gctgttctta
     3721 gcggaagagg ataccggcaa cgtgatgggc gcgttttccg cgcgcggtcc gggaccttcc
     3781 gaagacatcc tgaagcccga cgtcacggcg ccagggatca acatcctggc aggtttcacg
     3841 ccggacgcgg ccaactccgt gaccggggag aactttgcgt tccttacggg cacatcgatg
     3901 gctgcgcccc acgtggccgg cgtcgccgcg ctcctgcggc aagcgcatcc tgactggagt
     3961 ccagccgcca tcaagtcggc gcttttgacg actgcacacc aggacgtcaa ccagcaggac
     4021 ggtgtgaccg cggcgatacc tttcgatttc ggcagcggtc acatcgaccc caatcccgcg
     4081 aacaacccgg gcctcgttta cgacgcaacg gccgacgatt acgacgcgtt cgcctgcggc
     4141 acgttttcgc cggccgtgga cgaggcgcgg tgcgaccagc tcgcggcggc cggcttttcg
     4201 ttcgggcccg aggacatgaa ccagccttcg attgccttgt cacggctgac cggaacgcag
     4261 acggtgaccc gccgcgtcac caacgtcagc gtagagaccg gcagctatac ggcggagatc
     4321 gaagcaccgc cgggcatcag cgtgctggtc gcgccgtcca c
//
LOCUS       DASUOM010000454         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_656449,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000454 DASUOM010000000
VERSION     DASUOM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656449"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..530
                     /locus_tag="VFY27_09385"
     CDS             93..530
                     /locus_tag="VFY27_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5765768.1"
                     /translation="MQDLPHHYRAAATLDPENNVDLASPGLQNLESASPAEYGGPGDK
                     WSPETLLVAAVADCFVLTFKAIARASKFEWSSVKCDVEGLLDREDKVTRFVSFDIRAV
                     LDVPAGTDEARAKRLLEKAEQNCLITRSLTAHADLETEVRVTG"
     gene            complement(654..1499)
                     /locus_tag="VFY27_09390"
     CDS             complement(654..1499)
                     /locus_tag="VFY27_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765769.1"
                     /translation="MRIAASASALMVVMGSFALAADRPVFQARALAEFDAAEARQGVA
                     IDAGYFYAASNFAITKHDRRTGEQVGEWRGRSAGEPLIHLDSLVAHEGKLYASHSNYP
                     EWPMTSSVEIWNAETMEHIDTHSFGVYRGSFTWLDRHHGSWWGAFANYDKVQDGQAAA
                     YGETDRTQVVKMNDEFAVIESWIIPREILDRFRPMSNSGGSWGPDGFLYLTGHDLGEA
                     YVMRLPQAGSVLEWVATVQIPVMEGQGIAWDRSGNARHLWGIVKKDRKVLKIEMPPIN
                     LPASL"
     gene            1661..2398
                     /locus_tag="VFY27_09395"
     CDS             1661..2398
                     /locus_tag="VFY27_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5765770.1"
                     /translation="MKHRRYKQIADILAEKIYSGEYKVGQRLPPERDLAEQFSIGRPV
                     LREALIALELSGLVAVRQGAGIYVQPWQNQPLRVNIFDRGVSPFELIDARCLIEAEIA
                     AEAATIITDRELLGLQHAIQQMKDSFTNLEQYEKHDREFHILITKAARNTALTAVIDS
                     LWALHTRGALWNQLHRFTPPEKLHGDRLDEHLAIYDALKRRDANGAREAMRVHLKRAK
                     QTLMSAADALKEQGEREASGITGSGAG"
     gene            complement(2376..>3551)
                     /locus_tag="VFY27_09400"
     CDS             complement(2376..>3551)
                     /locus_tag="VFY27_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEX5765771.1"
                     /translation="GIVILFGAIIGVFLERSGGALRLAESILKRTGEKHIPAAMGGVG
                     YIVSMPVFCDTAFIILSSLNRALSRKAGVGIAAGAIALSLGLYTTHTMVPPTPGPVAA
                     AGILEADLGLVILWGMIVSLVALLAGWIFAVKVAAKVQVRDTPIAAAEVAAKSAESAE
                     AEHGSPPGVFAAIVPILLPIVLILLRSVAQLPGAPLGSGTLYTFLDFVGHPVIALLAG
                     VAFAFTLPPRFSRQMLSAGGWVGEAVAAAAPIIIITAAGGAFGRVLQGAEIGTVLQPY
                     VETLQMGVAIPFLAAAILKTAQGSSTVAIITSASLMAPFMAALGLDTDVGRALTVVAI
                     GAGSMVVSHANDSYFWVVTQFSGMDVKTGYKLQTLGTLVAGTAAGFSVWLLSLLLIQ"
BASE COUNT          728 a         1142 c         1064 g          617 t
ORIGIN      
        1 tgaactctcc ctgaccccgc ttgcattccc tcgtttgccc ccccatttct cgaaaacgcc
       61 agctacatgc cagccaagcc acggagaacc ccatgcagga ccttccccac cactaccgcg
      121 ccgcagccac tctcgacccc gaaaacaacg tcgacctcgc gagccccgga ctccagaacc
      181 tcgagtccgc gtcacccgca gaatacggcg gccccggcga caaatggtcg ccggaaacgc
      241 tgctggtcgc tgcggtagcg gattgcttcg tactcacctt caaggcaatc gccagggcgt
      301 cgaaattcga gtggtcgtcc gtcaagtgcg acgtcgaagg cctgctcgac cgcgaagaca
      361 aggttacgcg ctttgtgagc ttcgatatcc gtgcggtgct agacgtaccg gcaggcacgg
      421 acgaagcgcg cgcgaagcga ctgctcgaga aggcggagca gaactgcctg atcacgaggt
      481 cgctaacggc ccatgccgac ctcgaaaccg aggttcgcgt aacaggctag ggaatgaacg
      541 caggagccgg cgctgaacgg aatcggcaac aaattcgaac gctgattgcc gggcgggcga
      601 tggatgcggg cgagttcgcg aacgccatcg cgcccgcctg catggcagtc ttcctacaaa
      661 gacgccggca agtttatcgg cggcatctcg atcttcagga ccttccggtc cttcttcacg
      721 attccccaca agtgccgggc gttgccggag cggtcccagg ctattccctg cccctccatc
      781 accgggatct gcaccgtggc cacccactcc agtaccgagc cggcctgcgg tagtctcatc
      841 acgtaggcct cgccgaggtc gtggcccgtc aggtacagga aaccgtcggg cccccatgaa
      901 ccgccggagt tgctcatcgg ccggaagcga tcgagaattt cgcgagggat gatccaggac
      961 tcgatgacgg cgaactcgtc gttcatcttc acgacctgcg tgcgatcggt ctcgccatag
     1021 gcagcagcct gcccgtcctg caccttgtcg tagttggcga aagcccccca ccaggagccg
     1081 tggtggcggt ccagccaggt aaacgaaccc cggtagacgc cgaagctgtg ggtgtcgatg
     1141 tgttccatcg tctcggcgtt ccagatctcc accgaactgg tcatcggcca ttcgggatag
     1201 ttggaatgcg aggcgtaaag tttgccttcg tgggcaacca ggctgtccag gtggattaac
     1261 ggctcgcctg ccgagcgacc gcgccattcg ccaacctgtt caccggttct ccggtcgtgt
     1321 ttggtgatcg caaaattgct ggccgcgtaa aagtaaccgg cgtcgatggc gacgccctgc
     1381 cttgcctcgg cggcgtcgaa ctccgcgagc gcccgcgcct ggaagacagg ccgatcggcg
     1441 gcgagtgcaa acgagcccat cacgaccatg agcgcggacg cggatgctgc tatccgcatt
     1501 atcgaacatc ttttcatacg atcacctgcc gacgctgaca ccctgcccgg ggtgtgtcaa
     1561 tattggtcct acccatccgc atgtgccatc ggcaactcac gctacgtcgg ctcatcaata
     1621 acttcttggt gttattgatc atggtgaggg gagccagcaa atgaaacaca ggcgttacaa
     1681 gcagatcgcg gacatccttg ccgagaaaat ttactccggc gagtacaagg tcggccagcg
     1741 cctgccgccc gagcgcgatc tcgcggaaca gttttccatc ggccgcccgg tacttcgcga
     1801 agcgttgatt gcgctcgagc tctcgggcct cgttgcggtg cgccagggtg cgggcatcta
     1861 cgtgcagccg tggcagaacc agccgcttcg cgtaaacatc ttcgatcgcg gcgtcagccc
     1921 tttcgaattg atcgatgcac gttgcctgat cgaagcggag atcgccgccg aagcggcaac
     1981 catcattacc gatcgtgaat tgctcggctt gcaacacgcc atacagcaga tgaaggatag
     2041 tttcacgaac ctggagcagt acgaaaagca tgatcgcgag tttcacatcc tcataacgaa
     2101 ggccgccagg aacacggcgt tgactgcagt catcgacagc ctgtgggcac tgcacacccg
     2161 cggcgcgctg tggaaccagt tgcacaggtt cacgccgccg gagaagctgc acggcgatcg
     2221 gctggatgag cacctggcca tctacgatgc cttgaagcgc cgcgatgcga acggcgcacg
     2281 cgaggctatg cgcgtgcacc tcaagcgggc gaagcaaacg ctcatgtccg ccgccgacgc
     2341 gctgaaggaa cagggagagc gcgaagcatc cggaattact ggatcaggag caggctgagc
     2401 aaccagacgc tgaagcccgc ggccgtgcct gccaccaggg tgccaagggt ctgcagcttg
     2461 tatcccgtct tcacatccat gccggaaaac tgcgttacga cccagaagta gctgtcgttg
     2521 gcgtgactga cgaccatcga accggcgcct atcgcaacga ccgtcagcgc gcgtcccacg
     2581 tccgtatcca gtccgagtgc ggccatgaaa ggtgccatca ggctggccga ggtaatgatc
     2641 gcgacggtgg acgagccttg cgccgttttc agtattgctg cggccaggaa cggaatggca
     2701 acgcccatct gcagcgtctc gacgtacggc tgcagcacgg tgccgatctc tgcgccttgc
     2761 agcacgcgtc cgaaagcgcc acccgccgca gtgatgatga tgatgggtgc agcggccgcc
     2821 acggcttcgc cgacccagcc gccggcggac agcatctgcc ggctgaacct gggcggtaat
     2881 gtgaacgcga acgcaacgcc tgcgagtagc gcgattaccg ggtgcccgac gaaatcgaga
     2941 aacgtgtaaa gagttccgct gcctaagggt gcgcccggca gttgcgcgac tgaacgcaag
     3001 aggatcaaca cgatcggcag cagaatcgga acgatcgcgg cgaatacgcc cgggggcgag
     3061 ccgtgctccg cttcggccga ttctgcgctc tttgcggcaa cctccgcagc ggcgatgggc
     3121 gtgtcccgca cttgtacttt cgccgcaacc ttcaccgcga agatccagcc ggccagcaaa
     3181 gcgacgagcg agacgatcat cccccacagg atgacgaggc ccaggtcggc ttcgagaatc
     3241 cctgccgccg cgaccggccc gggcgtcggg ggcaccatcg tgtgcgtggt gtagagaccc
     3301 aggctcagcg caatggctcc cgcagcgatc cctacgcctg ctttcctgga cagcgccctg
     3361 ttcagggacg agaggatgat aaaggcggta tcgcagaaca cgggcatcga aacgatgtaa
     3421 ccgacgccgc ccatcgctgc cggtatatgc ttctcgccgg tccttttcaa aatgctttcg
     3481 gcaagacgca gcgcaccgcc ggaacgttcc aggaaaacgc cgatgatggc gccgaacagg
     3541 atcacgatgc c
//
LOCUS       DASUOM010000455         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1255130,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000455 DASUOM010000000
VERSION     DASUOM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255130"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VFY27_09405"
     CDS             complement(<1..1706)
                     /locus_tag="VFY27_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEX5765772.1"
                     /translation="MADTNDCYHCGEPVPSGSDLRARVGDTSEPVCCVGCKAVSELIH
                     ANGLSAYYDYRSRPDPEAGLRPQPSAWAHYDGGDMQSRYVHREGNSAETTVEIGGMYC
                     SACVWLLENALKRHGHTESVTVSPATRRAVIRWDAAAMPFSELLETLAAVGFRPRPLA
                     AGHAADPAHDEYRRALKRLLVAAAAGMQVMMFAVALYAGDFFGIDGSIEKFLRTVSLL
                     ISLPIVFYSARPFFSGAWRGLRARSPGMDLPVAIAIAAAFVASVHATLLDQGEIYFDS
                     VAMFVLFLSASRFLEMRARHRSGDQAYALASLLPDAAIRVCDDGSEQAVSIDSLRVGE
                     VVRIRAGDVMPVDGEVQSGCLLVDESMLSGESLPVRRTSGMQVLAGSINRDGIGNVRV
                     IRTGAGTSLAEVGRLLERAKADRPPVAQLADRIASYFVAGVLMLVALTGAMWSQIDPS
                     RAFEVILATLVVTCPCALALATPTALAAAATKLASTGFLLVRSRLLEVLGHRAVLIFD
                     KTGTLTEGRPTVQRTECYRDDWNAPRCLALAAAIETASTHVLARAFATHERAGEFTIG
                     EIV"
     gene            complement(1708..1911)
                     /locus_tag="VFY27_09410"
     CDS             complement(1708..1911)
                     /locus_tag="VFY27_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765773.1"
                     /translation="MAGKNAVDSQEPWQRNPWVWLIIGIPALTVAGCLLTIYLALTRP
                     DYLVSDYADAPPGVERAPGEPAS"
     gene            complement(1913..3304)
                     /gene="ccoG"
                     /locus_tag="VFY27_09415"
     CDS             complement(1913..3304)
                     /gene="ccoG"
                     /locus_tag="VFY27_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX5765774.1"
                     /translation="MNDNATAGYEYGKLYRSEGKIYPREITGRFATLRRLAMFVLLGV
                     FYLGPWLRWEGRQAVLFDLPARKFYIFGLTLWPQDFIYLALLLVIAAISLFFFTALAG
                     RIWCGYACPQTVWTEAFSWIESAIEGNRSRRMKLDKGPWNLEKAWRKTAKQACWIVLA
                     LFTGYTFVGYFSAIDELTVRLATFSAGPWEVFWVLFYGFATYGNAGFMREQVCKYMCP
                     YARFQSSMFDNDTLIVTYDVERGEPRGARRRGSDHKSQGQGDCIDCTLCVQVCPTGID
                     IRKGLQYECIACAACVDACDSVMDKMNYPRGLIRYSTERAMEHKNYRLLRPRTVVYGA
                     ILATLTLALVSSLALTKPVILDVIRDRNALYRDVGRAGIQNTYTVRVVNKHSTPHDYV
                     LKVGGIEGIRLESNASFSVAAESVFTLPVSVTAPHEYAAGGHAVVFYLESTDGSDIRI
                     SEESRFRGPLENL"
     gene            complement(3312..3620)
                     /locus_tag="VFY27_09420"
     CDS             complement(3312..3620)
                     /locus_tag="VFY27_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765775.1"
                     /translation="MADPEDSVQPEWSRDTQKMAVLSWMSFLTASACSVLFFAFVDPL
                     VLVEAINVHAIESRHAGYAIGFFFFWANGWISGWFTMRLIRRKRRGPAAGQYPGIGTR
                     "
     gene            complement(3623..4504)
                     /gene="ccoP"
                     /locus_tag="VFY27_09425"
     CDS             complement(3623..4504)
                     /gene="ccoP"
                     /locus_tag="VFY27_09425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX5765776.1"
                     /translation="MSSAWSWYVAIGTIVSMLACFWLVFWTNRQRSSPEEIKASEAHV
                     WDENIRELNNPLPMWWLYLFILTLVWGALYFLLYPGLGGFAGLKSWTQGGQYDAEMAL
                     AEERYGPIFAGFGAMPLIDVANEPEALSIGRSLFANYCAQCHGSGALGAPGFPNLTSG
                     AWQWGGTPEQIKETILKGRSGVMPALGAVFPDEESVDAMVSYVQTIPDGMDTASPAHS
                     RYMTLCIACHGADGGGMQVLGAPSLKDDVWLYGSSPQEIRKSIVEGRQGNMPPHANLI
                     GSDRAHILAAYIYSLSQ"
BASE COUNT          929 a         1485 c         1409 g          808 t
ORIGIN      
        1 acgatttcgc cgatcgtgaa ctcgccggct cgttcgtgcg tcgcgaacgc ccgcgccagt
       61 acgtgcgtcg acgcggtctc gattgccgcc gcaagcgcca ggcatcgggg agcgttccag
      121 tcgtcgcggt agcactccgt ccgctgcacg gtcgggcggc cttccgtgag cgttcctgtc
      181 ttgtcgaata taagcaccgc acggtgcccc agcacctcca gcaagcgcga ccgcaccagc
      241 aggaacccgg tggaagcaag cttcgtagcg gccgccgcga gcgcggtggg cgtagccagc
      301 gccagggcac agggacaggt gacgaccagg gttgccagga tgacttcaaa tgcgcgcgaa
      361 gggtcgatct gcgaccacat tgcaccggtg agcgctacca gcatcagcac gccggcgacg
      421 aagtagctcg cgatgcgatc cgcgagctgc gcaacaggcg gcctgtccgc tttggcgcgt
      481 tcgagcaggc gacccacctc cgcgagactg gtgccggcac cggtccggat tacccgcacg
      541 ttgccgatgc cgtcccggtt gatgctaccc gccagcacct gcattccgga ggtccgccgg
      601 accggcagcg actcgccgct aagcatagac tcgtctacca ggagacatcc ggattgcact
      661 tccccgtcca ctggcatgac gtcgcctgcc cggatacgga caacctcgcc cacccgcagg
      721 ctgtcgatgg aaaccgcctg ctcgctcccg tcgtcgcata cacggatcgc tgcatccggc
      781 agcaggctcg ccagcgcgta cgcctggtct cccgagcggt gccgtgcgcg catttcgaga
      841 aagcgcgacg cggagaggaa caggacgaac atcgcaacgg aatcgaaata gatctcgccc
      901 tggtccagca gggttgcatg gaccgaggcg acgaacgcgg cggcaatcgc gatcgccacc
      961 ggcaggtcca tccccggcga gcgtgcgcga agaccgcgcc atgcgccgct gaagaacggc
     1021 cgtgccgaat agaagacgat cgggagagag atcagcaggc tgaccgtgcg caggaacttt
     1081 tcaattgacc cgtcgatgcc gaagaaatca ccggcataaa gcgcaacggc gaacatcatg
     1141 acctgcatgc cggctgcggc ggctaccagc aatctcttca gtgcacgtcg atactcgtcg
     1201 tgcgccggat ccgcggcgtg accagccgcg agcgggcgtg gacggaagcc gacagcagcc
     1261 agtgtctcga gcagctcgct gaagggcatc gccgcggcat cccaacgaat cacggcgcgc
     1321 cgcgtggcgg ggcttaccgt gacggactcc gtatgcccat ggcgcttcag tgcattctcg
     1381 agaagccata cgcaagccga acagtacatg ccgccgatct cgacggtcgt ctcggcagaa
     1441 ttgccttcac gatgcacata gcgcgactgc atgtcaccgc catcgtagtg tgcccacgcc
     1501 gacggctgcg gacgcaagcc tgcctccgga tcggggcggc tgcggtaatc gtagtacgcg
     1561 cttaagccat tggcatggat cagctcggaa accgccttgc aacccacgca gcagaccggc
     1621 tccgacgtgt cgccgacacg cgcgcggagg tccgagccgg atggcacggg ctctccgcag
     1681 tggtaacaat cgtttgtgtc ggccatgtca ggacgccggc tcccccggcg cccgctccac
     1741 gccgggcggc gcatccgcat agtcgcttac caggtaatcc ggacgggtga gcgcgagata
     1801 gatcgtcaag aggcaccctg caacggtgag cgcgggtatg ccgatgatca gccataccca
     1861 cggattgcgt tgccagggtt cctgcgagtc gactgcattt ttacccgcca tgtcagaggt
     1921 tctccagcgg gccgcggaag cggctctcct cgcttatgcg gatatcgcta ccgtcggtgg
     1981 actcgagata aaagacgacc gcgtgtccgc ccgcggcata ctcgtgcggc gccgtgaccg
     2041 ataccggcaa ggtgaaaacg gactccgcag caacggaaaa gcttgcgttg gattcgagcc
     2101 tgatgccttc gatgccgcca accttcagga cgtaatcgtg cggtgtgctg tgcttgttca
     2161 cgacccggac tgtataggta ttctggattc cggcgcggcc tacatcgcga tacagcgcgt
     2221 tacggtcgcg aatgacgtcc aggattaccg gcttcgtcag cgcgagggac gacaccagtg
     2281 cgagggtcag cgtggccagg atagcgccgt acaccacggt gcgcggacgt agcaaccgat
     2341 agttcttgtg ctccatagcg cgttccgtgc tgtaacggat caggccgcgc ggataattca
     2401 tcttgtccat caccgaatcg cacgcgtcga cacaggcggc gcaggcaatg cactcgtact
     2461 gcaagccctt gcgtatgtcg atcccggtgg ggcaaacctg gacgcaaagc gtgcaatcga
     2521 tgcagtcgcc ctggccctgc gacttgtgat cgctgccgcg ccgtcgcgcg ccccggggct
     2581 ctccacgctc gacatcgtaa gtaacgatca gcgtgtcgtt gtcgaacatc gaactctgga
     2641 aacgagcgta cgggcacatg tacttgcaca cctgctcgcg catgaaacct gcattgccgt
     2701 aggtcgcgaa gccgtagaac aagacccaga aaacttccca cggtcccgcg ctgaacgttg
     2761 ccagacgcac ggtcagttca tctatggccg agaagtagcc aacgaaggtg tatccggtga
     2821 aaagcgcaag cacgatccag caagcctgtt tcgcggtttt cctccaggcc ttctcgagat
     2881 tccacggccc tttgtccagc ttcatccgtc ttgaccggtt gccctcgata gcggattcga
     2941 tccaggaaaa tgcctcggtc catacggttt gcgggcaggc gtagccgcac catatcctgc
     3001 cggcgagtgc cgtaaagaag aacaggctga tcgcggcgat cacgagcaac agcgcaagat
     3061 agatgaagtc ctgcggccac aacgtaaggc cgaagatata aaacttgcgc gcgggaagat
     3121 cgaagagaac tgcctgccgg ccttcccagc gcagccacgg ccccaggtag aaaaccccga
     3181 gcagcacgaa cattgccagc cgccgcagag tggcgaagcg acccgtgatt tctctcggat
     3241 agattttgcc ttcgctacgg tacagcttgc cgtactcgta gccggcggta gcgttgtcgt
     3301 tcatgcggtg ctcagcgcgt gccgatgccg ggatactgtc cggcagcggg tccgcggcgc
     3361 ttgcgcctga tgaggcgcat ggtaaaccag ccggatatcc agccgttggc ccagaagaag
     3421 aaaaaaccta tcgcataccc ggcatgacga ctctcgatgg catgcacatt gatggcttcg
     3481 accagcacca acgggtcgac gaacgcgaag aacagcacgg aacatgcgga tgccgtcagg
     3541 aacgacatcc aggagaggac cgccatcttc tgcgtatcac gggaccactc cggttggacg
     3601 ctgtcttcag ggtcggccat ggctactgcg aaaggctgta tatgtaggcg gcaagtatgt
     3661 gagcgcgatc cgagccgatc aggttggcgt gcggcggcat gttgccctgt cgtccctcga
     3721 cgatgctctt gcggatctcc tgcggtgagc tgccgtacag ccagacgtcg tctttcaggc
     3781 tgggagcgcc cagcacttgc atcccgccgc cgtctgcgcc gtgacaggcg atacagagcg
     3841 tcatgtaacg cgagtgagcg ggcgacgcgg tatccatacc gtcaggaatc gtttgtacgt
     3901 agctcaccat ggcgtcgaca gactcttcgt ccggaaacac cgcgccaagc gccggcatga
     3961 cgccgctgcg acctttcagg atagtttcct tgatctgttc cggcgtcccg ccccactgcc
     4021 aggcaccgga ggtcaggttc ggaaagccgg gtgcgcccag tgctcccgag ccatggcatt
     4081 gggcgcagta gttcgcaaaa aggctcctgc cgatggaaag cgcttccggt tcgttcgcca
     4141 catcgataag cggcatagcg ccgaagcccg caaagatggg accgtaccgc tcctccgcga
     4201 gcgccatctc cgcgtcatac tgcccgccct gcgtccagga tttcaggccg gcgaagccac
     4261 cgaggccggg gtacaggagg aagtacagcg caccccatac cagcgtgaga atgaacaggt
     4321 aaagccacca catcggcagc ggattgttca actcgcgaat attctcgtcc cagacgtgtg
     4381 cttcggacgc cttgatctct tccggactcg accgctggcg attggtccag aacaccagcc
     4441 agaaacaggc cagcatgctg acgatagtgc cgatagcaac gtaccagctc caggcgctgc
     4501 tcatttcatt cgctccgtgt ccttctgcgg cggtgcctcg tcgtcttcca gcggcatgcg
     4561 cgcggcctca tcgaattgtg acttgcgttt tcggctccac gtccagaccc agacggcgac
     4621 gaacagaacc a
//
LOCUS       DASUOM010000456         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1139634,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000456 DASUOM010000000
VERSION     DASUOM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139634"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(717..3494)
                     /locus_tag="VFY27_09430"
     CDS             complement(717..3494)
                     /locus_tag="VFY27_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX5765777.1"
                     /translation="MQVSKATRSVSHCLGILGILLTLGGCLSGGGEEEAAAPQLQRQQ
                     ITLTGSVGDGPVVGAAMAATTPTAEVIAEFESDTSAGYSVVLEVVSSDFPLILSATGG
                     TDLVTNLAPDFDLSGAALASDTAATANVNPFTTFAVEVANRLEGGPTSSNIAEAEDLV
                     VAALDTGLTTMLVQGPLASPVDETNIAEIVRASEALAEIVRRTRDHLAIPVNDVIAAL
                     GADLTDGIVDGVGPMPANPRIAAVWSLITAQVLLETLTNTLYVNGSNAMSAMEAAITR
                     VSTATPQPSLHDLTATTAMLSQTRVGLSAAWAITGDPDIADLAASIDRIRAGSSASLA
                     RIALPNDFQSRLNTAIGMVVSGDNSVVDTVNTVVRQGGTTITPNNNPPAISGNPPATV
                     EVGNPYDFAPTASDADGDALTFSIAGKPAWATFAASSGRLSGMPGINDVGTYADIRIS
                     VSDGSNSNSLAAFSIEVTNGNAAPTISGSPPLVVDVDAMYSFLPTANDADGDTLTFSI
                     SNSPAWAVFDSSTGGLSGTPGSSDVGSYLDIVITVSDGQQSASLGPFTVNVVADNTPP
                     SIAGSGAATLAAGTAYDFQPTASDPDGDPLSFTITNPPSWTSFDATTGRLSGTPAVAD
                     EGTFANIIIRVSDGTSTSSLAAFSITVTVPNTPPTIGGTPETQVNAGTTYSFTPTAND
                     ADGDDLSFSISGRPAWASFSQSTGQLSGTPGTGAAGTYSNIRISVTDGTATATLPAFS
                     ITVVAPNSAPTISGTPAAAVNVNTAYSFTPTANDPDGDTLTFSITGMPAWATFDTSSG
                     RLAGTPGDADVGVYGDIRITVSDGTGSATLGPFSITVNAVSNGSATLSWTAPTQNTDG
                     STLTDLAGYKLYWRIGSGAYQSPVTISNPSVTTYIVENLAPGTYEFVATAFNSQNVES
                     DHSASATITVP"
     regulatory      complement(3603..3687)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          726 a         1128 c         1210 g          826 t
ORIGIN      
        1 gtattggggt tactcgaccg cgtgtatgcg tcggcgactg ccgagaacgt ggcagcttgt
       61 gcggaatatc cgggtgtgaa gccggcagca accaccaccg cactcagtag agccttaata
      121 caagtccctc taagccgttg aaccaatggc ataaatatcg ctccttcaaa gctgcccaag
      181 cgcccgcatc gcggagcgcg aacaaacggt tcccgtgggc gagatagcgc gtgctatcgc
      241 ctgcaattaa tcgctgcttg tgcccaagca aatgtaacgg cgagctgcga gccattttca
      301 ggctgcgaca aactgcctaa accgtccgtt acgagttcct tcagatacga ttcggaatga
      361 aggctcgacc cggattatgt ttatagcggg aggctaccat cgtgcgacag aaaaaaaacc
      421 tgtctcaaat tgcagacgca gcaacgttac aaagctgtag aaacggtgcc gattcactcg
      481 acccgctgac gccaggtggc gtcgaatgcg ataaatcgcg agttggcact atcgatactg
      541 actgacgtac ctcgctctcg agccggcaga agactcaatc gatatatttg cgcgccgctt
      601 ttggtctagc gggccgatgc gaccgaatac gggtcgccaa tggagattgg cggtcatctg
      661 cggcgggacc gacggacggg gactggcttc catgcctcgc gtttttggct cggcgtttac
      721 ggcacagtga tggttgcact cgccgaatga tcgctctcga cgttctgcga attgaatgcc
      781 gttgccacga attcataggt gcctggtgcc aggttctcta cgatgtaggt ggtgacactc
      841 gggttgctga tcgttacggg actctggtac gcccccgagc cgatgcgcca gtacagcttg
      901 tatccggcga ggtcagtgag tgtcgacccg tcagtattct gtgttggcgc agtccatgac
      961 agcgtcgccg acccgttgct gactgcattt acggtgatcg agaacggtcc gagcgtcgca
     1021 gaccctgtgc catccgacac ggtgatgcga atgtcgccat agactccgac gtccgcgtcc
     1081 ccgggcgtgc cggccaggcg gccgctgctg gtgtcgaaag tcgcccaggc cggcatgccg
     1141 gtaatcgaga aggtcagggt gtcgccatca gggtcgttcg ctgtcggtgt gaagctgtag
     1201 gccgtgttga cattgacggc tgcagccggt gtaccgctga tcgtgggcgc gctattgggc
     1261 gccaccacgg tgatcgaaaa tgcaggcagc gtcgcggtag ccgtgccgtc ggtaacactg
     1321 atgcggatgt tcgaataggt accggcagcg ccggtgcccg gcgtacccga gagctggccg
     1381 gtcgactggc tgaaactcgc ccacgccggc cggccgctga tcgaaaagga caggtcgtcg
     1441 ccatcggcgt cgttagcagt cggggtaaag ctgtacgttg tcccggcatt tacctgtgtt
     1501 tccggcgtgc caccgatggt cggcggcgtg ttcggcaccg tcaccgtgat cgagaacgct
     1561 gccaggctgc tggtcgacgt gccgtcgctc acccggatga tgatgttcgc gaacgtgcct
     1621 tcgtcagcca ccgccggcgt accgctgagc cgcccggttg tggcatcgaa gctggtccac
     1681 gacggcgggt tcgtgatcgt aaagctcaat ggatcgccgt cggggtcgct ggcggtcggc
     1741 tgaaagtcgt acgccgtgcc ggctgcaagc gttgccgcac ccgaacccgc aatgctcggt
     1801 ggggtgttat ccgccaccac atttacggtg aacggcccga gactcgcgct ttgttgtccg
     1861 tccgatacgg taatgacgat atccagatag gacccgacat cgctgctgcc gggcgtcccg
     1921 gaaagcccgc cggtcgacga atcgaagact gcccaagcgg gcgaattcga gatgctgaat
     1981 gtcagggtgt cgccgtccgc gtcgttcgcg gtcgggagga acgaatacat tgcgtcgacg
     2041 tcaacgacca agggcggcga gccgcttatc gtcggcgcgg cattgccgtt cgtgacctct
     2101 atggagaacg ctgccaggct gttcgagttg ctgccgtcgg acacgctgat gcggatatcg
     2161 gcgtaggtgc cgacgtcgtt gatcccaggc atgccggaaa gacggccgct cgacgccgcg
     2221 aacgtcgccc aggccggttt gccggcgatc gagaatgtca gtgcatcacc atcggcatca
     2281 ctcgcggtcg gggcaaaatc gtacgggtta ccgacttcga cggttgcggg cggattgccc
     2341 gagatcgccg gtggattgtt gttcggcgtg atcgtcgtcc cgccctggcg aacgacggtg
     2401 ttcaccgtat cgacgacgct gttgtcgcca ctcaccacca tccctatggc ggtattcaag
     2461 cgggactgga aatcgttcgg tagcgctatt ctggccagcg atgccgaact acctgctcgt
     2521 atccgatcga tggacgctgc gagatccgcg atgtcgggat cgccggtgat tgcccaggcg
     2581 gcgctgagtc cgactcgagt ctggcttagc attgcggtcg tggcggtcag gtcgtgcagg
     2641 gacggttgcg gcgtagccgt cgataccctt gttatggccg cctccattgc cgacattgcg
     2701 ttggagccat tcacatacaa ggtgttggtg agtgtttcga gcagtacctg ggcggtgatg
     2761 agcgaccata ccgcggcaat tcgcgggttg gctggcatcg gaccaacgcc gtcgacaatg
     2821 ccatcggtaa gatcggcacc cagcgccgca atcacatcgt tcacgggaat cgccaggtgg
     2881 tcacgcgtcc ggcgaacgat ttccgcaagt gcctccgagg cgcgcacgat ttccgcgatg
     2941 ttggtttcgt cgacaggaga cgcaagcggg ccttgtacga gcatcgtcgt caggccggta
     3001 tcgagggcgg cgacgacgag atcctctgct tccgcgatgt tcgaggaggt gggtccacct
     3061 tcgagacgat tcgccacttc gacagcaaag gtcgtgaaag gattgacgtt ggctgtggcg
     3121 gcggtgtccg acgccagggc tgcaccgctc aggtcaaagt ccggtgcaag atttgtaacc
     3181 aggtccgtgc cgcccgtggc ggacaggatg agtggaaagt cggagctcac gacttccagg
     3241 acgacgctat atccggcact cgtgtcgctc tcgaattcgg cgatgacctc ggccgtagga
     3301 gtcgtagccg ccattgcggc accgaccacg ggaccatcgc cgacactgcc cgtcagagta
     3361 atctgctgtc gttgcaattg cggcgccgca gcttcctctt cgccgccgcc gctcaggcac
     3421 ccgccgagag tgaggagaat tcccagaatg cccaggcaat gacttactga cctggttgcc
     3481 ttcgatacct gcattgcgtg ctcctgcact gtgcagggca atccaagcat cggcgctagc
     3541 gctagtatgt gaaaccggtg ttggcaagcc ttccggcgcg ccgcgacaca agctagctca
     3601 atactggcag ccccgctgtc ataggcattt gcccttagac cggttgcttt gcgtgccagt
     3661 ctttcgactg gattgccctt ttcagtttga gtttccttcc tcgtcgagct tgaccttcgc
     3721 tggtcgacgt ttgtatcccg catccaagtt agcacgttca tcgtgcgatt gaacctgtcg
     3781 caaaaaatcg acagtaggaa tttggcgcgt catttgtcgc tttgcaatat cttgtttttt
     3841 ctaaggatta tctttgttca cagcagggga ccggtgcctt cgacaaacta
//
LOCUS       DASUOM010000457         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1328022,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000457 DASUOM010000000
VERSION     DASUOM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328022"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFY27_09435"
     CDS             <1..279
                     /locus_tag="VFY27_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5765778.1"
                     /translation="MGGMAGNGSGDIFIAFSTSVPEEQEGKGTMVRMHANDTISPLFD
                     ATVLATEEAIVNAMVAAKDMTGDRGHVVKAIDHDELRNVLKQYNRLAD"
     gene            295..1725
                     /locus_tag="VFY27_09440"
     CDS             295..1725
                     /locus_tag="VFY27_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HEX5765779.1"
                     /translation="MSDTAVVLVTLVLYNAILVGVGLWARKRNESIEDFYLGGRRLGP
                     WVAAISASASSSSAWTLLGVSGAAYAWGLPALWLFPATVGGFLVNWVWVAPRLKSLSA
                     EEGALTLSAVVAPAGLGEARGAILKVAAVIIVFSFTFYIASQFEAAGKAFESAFQLSK
                     QASILVGAAVVLSYTLLGGFWAVSVTDLVQGLLMATAAVLLPLAALIAVGGVGPLLDG
                     LAAAGGPSIPAGQFSGLTGVFFVLGMFGITIGYPGQPHVVNRYMALRDEKSLRQGRVI
                     AMGWAVIVYTGMLLLGLSARVLFADIGDAEQVFFQTAQRLLPAVLAGVMLAAVLSAIM
                     STADSQVLVTASSISHDWNLAGGRSGSGLLRSRLTVVVVLTMATALALLWRADIFSRV
                     LFAWVALGAAFGPLLVLRLAGRSVSARGTLAAMLAGFTSTLVLSWFPNAPGDVAERIL
                     PFVFAFAIAIPASRRVTDRQQVQEGT"
     gene            1848..>2240
                     /locus_tag="VFY27_09445"
     CDS             1848..>2240
                     /locus_tag="VFY27_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765780.1"
                     /translation="MKLLISCSAAALLFLSGCDRGEETPTPQPAEPVAAQQQEEDASA
                     ELNALVEEFFERQLELNPLYATAIGDLRFNDRMANQIGPEHREATAALNREYLERVLA
                     LDPEILQGQDRLTYETFRYDREMELEELE"
BASE COUNT          362 a          690 c          736 g          452 t
ORIGIN      
        1 atgggcggca tggcaggcaa cggttcgggc gacattttca tcgcgttctc gacgtcagtt
       61 cccgaagaac aggaaggaaa gggcacgatg gtgcgcatgc acgccaacga cacgatcagc
      121 ccgctgttcg atgcgacggt actcgcaacc gaggaagcga tcgttaacgc gatggttgcc
      181 gcgaaggaca tgacaggtga tcgcgggcat gtggtcaaag cgatcgacca cgacgagttg
      241 cgcaacgtcc tgaagcaata caaccgcctg gccgattagc gggcaacact cgaaatgagc
      301 gatacggcgg tcgtccttgt cacgcttgtc ctgtacaacg cgatccttgt aggtgtcggc
      361 ctctgggcgc gcaagcgcaa cgagagcatc gaggatttct atctcggggg gcgccggctg
      421 ggaccgtggg tagcggcgat cagcgcatcc gcaagttcgt cttccgcctg gacgctgctc
      481 ggcgtcagcg gcgcagcata cgcctggggg ctgccggcat tgtggttgtt ccctgcgacc
      541 gtcggcggct tcctggtcaa ctgggtgtgg gtggcgccgc gactgaagtc gctctcggcc
      601 gaggagggcg cgcttacgct cagtgccgtg gtcgcgccgg ctgggcttgg cgaggcgcgc
      661 ggcgcgatcc tcaaagtcgc tgcggtaatc atcgtgttca gtttcacttt ctacatcgcg
      721 tcgcagttcg aggcggccgg caaggcattc gaatcggcat ttcaactgtc gaagcaggca
      781 agcatcctgg tcggcgccgc agtcgttctc tcctacacgc tgctgggcgg cttctgggca
      841 gtgagtgtga ccgacctcgt gcagggtctg ctgatggcca ctgcggcagt tcttttgccg
      901 ctggccgcgc tcatcgccgt cggcggtgtg ggaccgcttc tcgacggtct tgcggcggct
      961 ggcggtccgt cgattcctgc gggacagttc agcggactga caggcgtttt cttcgtgctc
     1021 ggcatgttcg gcataacgat cggttatccc gggcaaccgc acgtcgtgaa ccgctacatg
     1081 gcgctgcgcg atgagaagag cctgcggcag ggccgcgtca tcgccatggg ctgggcggtg
     1141 attgtctaca ccggaatgct gttactgggg ctgtcggcgc gggtgctgtt cgcggacatc
     1201 ggcgatgccg agcaggtgtt cttccagacc gcgcagcggc tgctgcctgc cgtactcgca
     1261 ggtgtcatgc tcgcggcggt gctctccgcc atcatgtcca cggccgatag ccaggtactt
     1321 gtcaccgcat catcgatctc gcatgactgg aacctcgccg gcggacgttc cggcagcggg
     1381 ctcttgcggt cgcgcctgac ggtcgtcgtc gtattgacca tggcgaccgc gctcgcgctc
     1441 ctgtggcggg cagacatctt ctcgcgcgtg ctttttgcct gggtggcgct cggtgcggca
     1501 ttcggtcctc tgctcgtcct taggcttgcc gggcgctcag tctctgcccg cggcacgcta
     1561 gccgcgatgc tggccggttt cacttcaacg ctggtattgt cgtggttccc gaacgcccct
     1621 ggcgatgtcg ccgagcgcat ccttccgttc gtgttcgctt tcgcaatcgc gattccggcg
     1681 agccgccgcg tcaccgaccg gcagcaggtt caggaaggca cctgaggaac tttgcaccga
     1741 ctgaggcgat cgcactggtc tgcgacttta ctggagcggc cgatgccgcc atcatgaacg
     1801 ccttttggcg cgggccataa cctgcgacga actccgggaa gtcctgaatg aagctactga
     1861 tatcctgctc tgctgcggcg ttactgttcc tgtcaggctg cgatcgcggc gaagagacgc
     1921 cgactccgca accggccgag cctgttgctg cccagcagca ggaggaggac gcctccgcgg
     1981 aactcaacgc cctggtcgag gagttcttcg agcggcagct cgagctgaat ccgctttatg
     2041 cgactgccat cggcgatctc cgcttcaacg accggatggc gaaccagatc gggccagagc
     2101 accgcgaagc caccgcagcc ctgaaccggg agtatctcga acgagtgctc gcgctggacc
     2161 cggaaatact ccagggccag gaccgcctga cctacgagac cttccgttac gaccgtgaga
     2221 tggagctcga ggagttagag
//
LOCUS       DASUOM010000458         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_53113,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000458 DASUOM010000000
VERSION     DASUOM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53113"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFY27_09450"
     CDS             complement(<1..263)
                     /locus_tag="VFY27_09450"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX5765781.1"
                     /translation="MEKFSKNPDAIAKLTPAQYRVTQQNGTEPPGTGEYLYNKEPGIY
                     VDIVSGEPLFASSDKFESGCGWPSFTKPIEPVNINEIRDTSHGT"
     gene            complement(328..403)
                     /locus_tag="VFY27_09455"
     tRNA            complement(328..403)
                     /locus_tag="VFY27_09455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Gly,seq:gcc)
     gene            complement(503..1063)
                     /gene="pgsA"
                     /locus_tag="VFY27_09460"
     CDS             complement(503..1063)
                     /gene="pgsA"
                     /locus_tag="VFY27_09460"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5765782.1"
                     /translation="MKANLANTLTWIRIAAIPVVAYCFLSTFQYSRPIAFVVFAVAAI
                     TDLLDGYVARRLGQTSKFGEFLDPVADKLMVAVVLVLLVQGDPRVVVALTAAVIIGRE
                     ITVSGLREWMASIGERAHVAVSAGGKVKTTLQMVGIALMVYQQPAFGVDIYDAGFLFL
                     LIAAGMTLWSMIIYLRAAWPSMNSKS"
     gene            complement(1094..2935)
                     /gene="uvrC"
                     /locus_tag="VFY27_09465"
     CDS             complement(1094..2935)
                     /gene="uvrC"
                     /locus_tag="VFY27_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5765783.1"
                     /translation="MTKDQPAEFDSKTFLRSLSHRPGVYCMLNAKGKVIYVGKARDLR
                     KRVSSYFQRAHADAKTTSLMQHVANVDVTITNTEAEALLLEYNMIKRHKPRFNVVLRD
                     DKSYPYIHVATDHEFPRIEFHRGARTGKGRYFGPYPNARAVRKTVNELQKLFLIRSCQ
                     DSYFSNRNRPCLQYQIKRCTAPCVHYIDSETYREDVNAAILFLEGKNRSVIEALVARM
                     ERAASQQDYEQAARYRDQIKRIKSVEAEQLVSRNYGKDIDVIASASKHGVYCVTVLFI
                     RNGAVLGSRNHFPKIAGDPDKEEVMSGFLSQYYLGREAPGEIIVDAVIEDEELLEQGM
                     SQQSNHKVEIRHRVRGDRLSWVQMARTNAEQGLSLQIASNATIRNQLETLAEALDLDG
                     VPERIECFDVSHTSGEATVASCIVFNQGGPLTSDYRRFNLTPEAAGDDYAAMREALQR
                     RYARVKKGEVPIPDLLLIDGGKGQLAEAMTVLDELELDRVRVVGVAKGRSRRPGQEQL
                     FLAGRSTPTILPPDSAALLLIQRIRDEAHRFAITGHRKRRTKARTTSRLERIPGLGPK
                     RRRELLRQFGGLQGVRSAGVGDLAKVHGISRNLAQRIYDDLHLDGNS"
     gene            complement(2932..3579)
                     /locus_tag="VFY27_09470"
     CDS             complement(2932..3579)
                     /locus_tag="VFY27_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5765784.1"
                     /translation="MDERIFNKDCRLCPRLSTFLDSVQDRYPDYYCRPVGPFGDIDAR
                     LLIVGLAPGMHGANRTGRPFTGDHAGILLYETLYKYGFSNQPQSISADDPLALIDCRI
                     TNCVKCLPPDNKPVGSEINTCNRYLVRELELLPPPKVVLALGGIGHRAFIRALGLRQA
                     DYKFAHAAEHRIQGRFLLLDSYHCSRYNTNTGRLTTAMFEAVFARAKELISRDET"
     gene            complement(3609..>4520)
                     /locus_tag="VFY27_09475"
     CDS             complement(3609..>4520)
                     /locus_tag="VFY27_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5765785.1"
                     /translation="GLVDMVVFRENTEDIYAGIEYPSGSQEVQKLIHFLQQELKVKEI
                     RFPASSGIGIKPVSREGSQRLVRKAIQHCIDRDFTSVTLVHKGNIMKFTEGAFCQWGY
                     ALAREEFGAMPKDGGPWLTFNNPVTGNEIIIKDVIADNFLQQILMRPEDYDVIATLNL
                     NGDYISDALAAQVGGIGIAPGANIGEGVAVFEATHGTAPGFAGKDRVNPGSLILSAEM
                     MLRYIGWREAADFIVKGVANTIANKELTFDLARLREAIHRPKRKERPHGKKDVEHELD
                     QLIPGATLVGTRGFGEAVIRHMDDPED"
BASE COUNT          786 a         1381 c         1391 g          962 t
ORIGIN      
        1 gtgccgtgcg aggtatcccg gatttcattg atgttcaccg gttcgatcgg cttggtgaaa
       61 ctcggccagc cgcagcccga ctcgaacttg tcggatgagg cgaagagcgg ctcgcccgaa
      121 acgatgtcca cgtagatccc cggctccttg ttatacaggt attcgccggt cccgggaggc
      181 tcggtgccgt tctgctgcgt cacgcgatat tgcgcggggg tgagcttcgc aatcgcgtca
      241 gggttcttgc tgaatttctc cattaccgtc tcctcgaggc gcagtaactc atatggaggc
      301 tgcgggccgc tgattcaacg caggaactgg agcgggtgaa cgggctcgaa ccgtcgacct
      361 gtaccttggc aaggtaccgc tctaccaact gagctacacc cgctaaatcg gaggaaattc
      421 cgttctggcg tgtccaattc cgcccgccgg caggcggagg gctggaaagt ttagcctgtg
      481 gccgggtagt gtcaagaccc catcaggact tgctgttcat cgagggccag gcggcgcgca
      541 ggtaaatgat catcgaccag agggtcatgc cggcggcaat caggagaaac aggaatccgg
      601 cgtcgtagat atcgaccccg aaagcgggtt gctggtagac catcagggcg atgcccacca
      661 tctgcagcgt ggtcttgacc ttgccgccgg ccgataccgc cacgtgagcc cgctcgccga
      721 tgctcgccat ccactcgcgc agtccggaga cggttatctc gcgcccgatg attacggccg
      781 cggtcagcgc cacgaccacc cgcgggtcgc cctgcaccag cagcacgagc acgacggcga
      841 ccatcagctt gtccgccacc gggtcgagaa actcaccgaa cttcgaggtc tggccgagcc
      901 gtcgcgccac gtacccgtcc agcaggtccg taatagcggc aacggcgaaa acgacaaacg
      961 cgatcggccg cgaatactgg aacgtgctga ggaagcagta cgccaccacc ggaatcgccg
     1021 cgatgcggat ccatgtaagc gtgttggcaa ggttcgcttt cactgcgaca tctgcgttac
     1081 gggatgcacc ccgtcagcta ttgccgtcga ggtgcaggtc gtcgtaaatc cgctgcgcga
     1141 gattgcggct gattccatgg accttggcga ggtcgccgac gccggcgctg cgaactccct
     1201 gcaagccacc gaactggcga agcagttccc ttcgccgttt gggaccgagg ccgggaattc
     1261 gttccagtct cgatgtcgtt cgcgctttcg tgcggcgctt gcggtgccct gtgatggcaa
     1321 atcgatgggc ttcgtctcgg attctctgta tcaggaggag cgcggcggaa tcaggcggca
     1381 gtatagtggg cgtgctgcgg cctgccaaga acagctgttc ctgtccagga cgtctcgacc
     1441 ggcctttcgc cacgcccacg acgcgtaccc ggtccagctc cagttcgtcc agcaccgtca
     1501 tcgcttccgc aagctgacct ttgcccccgt cgatgagcaa caggtccggg atgggcactt
     1561 cgcctttctt tacccgcgca taacgccgct gaagcgcttc acgcatggcg gcataatcgt
     1621 cgcccgccgc ctccggagtc aggttgaagc gccggtagtc gctggtcagc ggcccgccct
     1681 ggttgaacac gatgcaggac gcaacggtcg cttctcccga ggtgtggctt acgtcgaagc
     1741 attcgattcg ctccggtacc ccatcgagat cgagggcctc ggcgagcgtc tccagctggt
     1801 tgcgtatcgt cgcattgctc gcgatctgca ggctcaggcc ctgctccgcg ttcgtccgcg
     1861 ccatctgtac ccagctcagc cggtcgccgc gcacccggtg cctgatctcc accttgtgat
     1921 ttgattgctg cgacatgccc tgctccagca gttcttcatc ctcgatcact gcatcgacga
     1981 tgatctcgcc cggagcttcg cgacccaggt agtactgcga caggaagccg ctcatgacct
     2041 cttccttgtc cgggtccccg gctatcttcg ggaagtgatt gcggctgccg agcaccgctc
     2101 cattgcggat gaacagtacg gtgacgcaat agacgccatg cttcgaggcc gaagcgatga
     2161 cgtcgatgtc cttgccgtag ttgcgcgaca ccagctgctc ggcttccacc gacttgatac
     2221 gctttatctg gtcgcggtaa cgcgccgcct gctcgtaatc ctgctggctc gcagcacgtt
     2281 ccatgcgcgc gaccagcgct tcgatcacgc tccggttttt gccttccagg aacaggatgg
     2341 ccgcgttgac gtcctcgcga tacgtctcgc tgtctatata atggacacag ggcgcggtac
     2401 agcgctttat ctggtactgc aggcagggac ggttgcggtt gctgaaatag ctgtcttggc
     2461 aactgcggat caggaacagt ttctgcagct cgttcaccgt ctttctcacc gcgcgtgcat
     2521 tcggatacgg gccgaaatag cgtcctttcc cggtccgcgc accccgatgg aactcgatgc
     2581 gggggaattc atggtctgtc gcgacgtgga tgtagggata gctcttgtcg tcacgcaata
     2641 caacgttgaa gcgcggcttg tggcgcttga tcatgttgta ttcgaggagc agggcttctg
     2701 cttccgtgtt cgtaatggtc acgtcgacgt tggcaacgtg ctgcatcagc gacgtcgtct
     2761 tcgcgtccgc atgcgctcgc tggaagtagc tcgagacgcg cttccgcaag tctcgtgcct
     2821 tgccgacgta gatgaccttg cccttcgcgt tgagcatgca gtagacgcct ggcctgtggc
     2881 tcaggctgcg gaggaatgtc ttcgagtcga actctgcggg ctggtctttg gtcacgtttc
     2941 gtcccgggaa atgagctcct ttgcgcgggc aaatactgcc tcgaacatgg cggtcgtcaa
     3001 tcgtccggta ttggtgttgt aacggctgca gtggtaggag tcgagcagca gaaatcggcc
     3061 ctggatgcga tgctccgcgg cgtgggcgaa cttgtagtcc gcctgcctca ggcccagtgc
     3121 cctgatgaac gcgcgatgac cgatgccgcc gagcgccagt acgacctttg gcgggggcaa
     3181 aagttcgagc tcgcggacca ggtacctgtt gcaggtattg atctcgctgc cgaccggttt
     3241 gttgtccggc ggcaggcatt tgacgcaatt cgtaatgcgg caatcgatga gtgcgagcgg
     3301 atcgtcggcg gaaatcgact gcggctggtt gctgaaaccg tacttgtaca gggtctcata
     3361 caggagaatg ccggcgtggt caccggtgaa ggggcggccg gttcggttgg ccccatgcat
     3421 accgggcgcg aggcccacga tgagcaggcg cgcatcgata tcgccgaagg gccctacggg
     3481 acggcagtag tagtcgggat acctgtcctg cacgctgtcc aggaaggtcg agagccgcgg
     3541 gcacaagcgg cagtccttgt tgaagatcct ctcgtccatc cggtatttcg caggtcacgc
     3601 gccgcgagtc aatcctccgg gtcgtccatg tgccggatta ccgcctcgcc gaagccgcgc
     3661 gttccgacca gcgtcgcccc gggaatcagc tggtcgagtt cgtgctcgac atccttcttg
     3721 ccgtgcggac gttccttgcg cttgggccgg tgtatggctt cacgaagccg cgcgaggtcg
     3781 aatgtgagtt ccttgttcgc gatggtgttg gcgacaccct tgacgatgaa gtctgccgct
     3841 tcgcgccagc cgatgtaacg cagcatcatc tctgcggaga gaatcagcga acccgggttg
     3901 acgcggtcct tgccggcgaa gcccggcgcg gtgccgtgcg tggcttcgaa caccgccaca
     3961 ccctcgccaa tgttcgcgcc gggcgcgatg ccgatgccgc ccacttgcgc tgcgagggcg
     4021 tcggagatgt agtcgccgtt caggttcagc gtcgcgatca catcgtaatc ctcggggcgc
     4081 atcaggatct gctgcaggaa gttgtcggcg atgacgtcct tgatgatgat ctcgttacct
     4141 gtcaccgggt tgttgaacgt gagccacggc ccaccgtcct tcggcatcgc gccgaactcc
     4201 tcgcgcgcca gcgcataacc ccactggcaa aaggctcctt ccgtgaactt catgatgttg
     4261 cccttgtgta cgagggtcac ggaggtgaag tcacgatcga tacaatgctg gatggctttt
     4321 cgcaccaggc gttgcgaccc ttcgcgcgat accggtttta tgccgatgcc ggaactggcc
     4381 gggaaccgga tctccttcac cttgagttcc tgctgcagga agtggatcag tttctgtact
     4441 tcctgcgatc cgctgggata ttcgatgcct gcatagatgt cttcggtgtt ctcgcggaag
     4501 acgaccatgt cgacaaggcc
//
LOCUS       DASUOM010000459         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1141008,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000459 DASUOM010000000
VERSION     DASUOM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141008"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFY27_09480"
     CDS             <1..1062
                     /locus_tag="VFY27_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765786.1"
                     /translation="GNVGLRHVRTDTKSVGIQLVGDVDGDGIPDGIPITDDLGVTAND
                     YAPVKYGPEYIDNLPSLNLSFHLTDNDQIRFAAAKVMARPPVFQLRGGAGSWTDTAND
                     GVSPRYNVWAKGNPNLDPFRADQFDLSYEHYFEDGGAFVAAVFWKDIESLVENITYTE
                     SDFGGDFTWEDIGLQAPPGFVEGQYETTRNNDNGGYIRGIELAYTTTFDGLPGVFAGL
                     GINANYAYTESETTINGGGNFPDQELPLPGLSKNVWSTTVFWDIDRFSTYVNARYRDE
                     YVFSGTSPGGASLQWADEYLVMDWQAAYLFDNGIGLVAQVNNLTDEVNSTNFGSQWAV
                     GEAKSFGRQFYLGINYKTN"
     gene            1171..2763
                     /locus_tag="VFY27_09485"
     CDS             1171..2763
                     /locus_tag="VFY27_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX5765787.1"
                     /translation="MRSGDDIRRSIVILGGGTAGWMAANLMAARWADRGFRITLIEAP
                     DIGIIGVGEGSTPQLKGFMDSIGVDEADWMPACNATYKAGIDFRGWSSRAGFASYFHP
                     FPAQPDDYTAPAFFYNSYVRRQGVDVEGHPDHFFLATYLAKHKLAPIAPEHFPFEINY
                     GYHFDSGLLGQFLGQVAQARGVEHMRGKVTDVRLGETGNIDAVLTDDGRLLEADVFVD
                     ATGFRGLLIQQSLKVPFASFSGNLFNDAAVVMPTPVEEDLECQTISTAMKYGWAWRIP
                     LSHRIGNGYVYSSDFVSSDEAEKELREHLGMLESDVQARHLKMRVGRVEQHWAQNCLA
                     VGLSQGFIEPLEATALHLVQETVVGFIENYEAGDFTNRYQQRFNDSINERFENVRNYI
                     VCHYRVNSRTDTEFWIENGRNEQLSQSLRSVLETWVGSKNLSDELARQKIDDYYPSIS
                     WHCLLAGYGLYPTDAQLKPGNELAHKYRLPALHDFIRRCAMNFRPHKEVIAAMQAEHP
                     TRPLHEPASRGPDVDRLYRRVR"
     gene            2804..3250
                     /locus_tag="VFY27_09490"
     CDS             2804..3250
                     /locus_tag="VFY27_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5765788.1"
                     /translation="MRGFPLPLAAVLSFWLPLTAHAEDSVDRAAIDEANARLIALQKQ
                     GNAEGMGDMYTDDAILLPSGAERADGREAIQAFWAKNLGSGVEEVELKTETLESLGDD
                     LVYEIGSYKTTPKDAEPVTGYYLVLWKRVDGRWKLHVDIFNENGGQ"
     gene            3343..3801
                     /locus_tag="VFY27_09495"
     CDS             3343..3801
                     /locus_tag="VFY27_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5765789.1"
                     /translation="MAASVKAIPDGYEAVIPYLVVADGAGAIDFYKKVFGATEIMRYE
                     DNGKVGHAELRIGRGILMVADEYPEMGYRSPKKGELPPAGMMIYVEDVDKTVRRALDE
                     GATEERPVADQFYGDRAGGIVDPYGHRWYIATHIENVSPEEMEKRAAANR"
     gene            complement(3919..>4278)
                     /locus_tag="VFY27_09500"
     CDS             complement(3919..>4278)
                     /locus_tag="VFY27_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5765790.1"
                     /translation="RTDSTAAMSFWFIAMIAVGAGALALPDWQPVRSSDALWLVGMGV
                     TGALGQLLITEAFKTAPVSVVAPFEYSTLFWGVLLDLAIWGDLPGPLVFAGAAVIVGS
                     GLYLIHRERKPTLLVPP"
BASE COUNT          930 a         1259 c         1326 g          763 t
ORIGIN      
        1 ggcaacgtcg ggctgcggca cgtccgcacc gacacgaagt cggtcggcat ccaactcgtc
       61 ggcgacgtag acggcgatgg cataccggac ggaattccga ttaccgacga cctcggcgta
      121 acagccaacg attatgcgcc ggtcaagtac ggacccgaat atatcgacaa cctgccgtcg
      181 ctgaacctct cgttccacct tacggacaac gaccagatcc gtttcgcggc ggcaaaggtc
      241 atggcccgtc cgccggtgtt ccagttgcgc ggcggcgccg gttcatggac agatacggca
      301 aacgacggcg tcagcccgcg ctacaacgtg tgggcgaagg gtaacccgaa cctggatccg
      361 ttccgcgccg accagttcga cctgtcctat gagcactatt tcgaagacgg cggtgcattc
      421 gtcgctgcag tgttctggaa ggacatagag tcactggtcg agaacattac ctataccgaa
      481 agcgacttcg gcggcgactt cacgtgggag gatatcggcc tccaggcacc tccgggtttc
      541 gtggaagggc agtacgagac gacccggaac aacgacaacg gcggctacat ccgtgggatc
      601 gaacttgctt acaccacgac cttcgacggg ctgccgggcg ttttcgcagg cctcggaatc
      661 aacgcgaact acgcctacac ggaaagcgaa acgacgatca atggcggcgg gaacttccct
      721 gaccaggagc tgccgttacc gggcttatcg aaaaacgtct ggagcacgac tgtgttctgg
      781 gatatcgatc gtttcagcac gtatgtgaac gcgcgttacc gcgacgagta cgtattctcc
      841 ggcacgtcgc cgggcggcgc gtccttgcaa tgggcagacg agtacctggt catggactgg
      901 caggcggcat acctgttcga caacggcatc gggctggtcg cacaggtcaa taacctgacc
      961 gacgaggtca acagcacgaa cttcggttct cagtgggccg tcggcgaagc gaagtccttt
     1021 ggccgccagt tctacctggg catcaactat aagaccaact agacgaccaa ctagccggct
     1081 gagtgcccct ccccccgggg ccccgccgcg actggaggcc gcccgcaatc aaatattgcg
     1141 ggcggctttt tctttgcgga gaatgacctg gtgcgatccg gggacgacat caggcgcagc
     1201 atcgtaattc tcggcggcgg caccgccggg tggatggcgg ccaacctgat ggctgcgcga
     1261 tgggcggacc gcggcttccg catcactctt attgaagctc cggacatcgg tatcataggc
     1321 gttggagaag gttcgacgcc gcagctcaag ggcttcatgg attccatcgg cgtcgatgaa
     1381 gccgactgga tgccggcctg caatgcaact tacaaagccg gtatcgattt tcgcggctgg
     1441 tcctcacggg ccggttttgc aagctacttc cacccgttcc cggcacagcc ggacgattac
     1501 acggcgccgg cgttcttcta taacagctac gtccgccggc agggcgtgga cgtggagggt
     1561 caccccgatc acttcttcct ggcgacgtat ctcgcaaagc acaagctcgc tccgatcgca
     1621 ccggagcatt tcccgttcga gatcaactac ggctatcact tcgactcggg gctgctcggg
     1681 cagttcctgg gccaggtagc acaggcccgc ggagtagagc acatgcgcgg gaaggtgacg
     1741 gatgtgcgac tcggcgagac cggcaatatc gacgccgtac tgaccgacga cggtcgcctg
     1801 ttggaggcgg atgtgttcgt cgacgcgacg ggcttccggg gacttctgat ccaacagtcg
     1861 ctcaaggtgc cgttcgcaag tttttccggg aacctgttca acgatgccgc cgtcgtcatg
     1921 ccaacaccag tggaggaaga cctggagtgc cagacgattt ccaccgcgat gaagtatggt
     1981 tgggcctggc gaatccccct ttcgcaccgc atcgggaacg gctatgtcta cagctcggat
     2041 ttcgtgagtt cggacgaggc cgagaaggag cttcgcgagc atctcggcat gctggaatcg
     2101 gacgttcagg cgcggcacct gaagatgcgc gtcggccggg tcgaacaaca ttgggcgcaa
     2161 aattgtcttg cagtcggact ttcgcagggc ttcatcgagc cgctcgaagc gactgcactg
     2221 cacctcgtgc aggaaactgt cgtaggtttc atcgaaaatt atgaggcggg tgattttacg
     2281 aaccggtacc agcagcggtt taacgacagt atcaacgagc gcttcgagaa cgtgcgcaac
     2341 tacatcgtct gtcactaccg cgtgaactca cggaccgaca cggagttctg gatcgagaat
     2401 gggcgtaacg agcagttgtc ccagtcgctc cggagcgtgc tggagacctg ggtcggcagt
     2461 aaaaacctga gcgacgaact cgcccggcag aaaattgacg actactaccc gtccatatcc
     2521 tggcattgcc tgctcgcggg ctacgggctg tacccgacgg atgcgcaact gaagccggga
     2581 aacgagctcg cgcacaagta caggctgccc gcacttcatg actttatccg ccgctgcgcc
     2641 atgaacttcc ggccgcacaa ggaggtcatt gccgcaatgc aggctgagca tccgacgcga
     2701 cctctacacg aacctgccag tcgcggcccc gatgtcgacc gcctctatcg cagggttcgc
     2761 taatctgcat agatggatgt gttatagcga ttggagagag cacatgagag gattcccgtt
     2821 gccgcttgcc gcggtacttt cgttctggtt gcccttgacg gcgcacgcgg aggacagcgt
     2881 ggacagagca gcaattgacg aggccaatgc gcggctcatc gctttgcaga agcagggcaa
     2941 cgccgaaggc atgggcgaca tgtacaccga tgacgcgata ctgcttccgt ccggagctga
     3001 acgggcagac ggacgcgaag ccattcaggc tttctgggcg aagaacctcg gctcgggcgt
     3061 cgaagaagtg gaactgaaga ccgaaacgct cgagtcgctg ggcgacgatc tcgtctacga
     3121 gatcggcagc tataaaacta cgcccaagga tgccgaaccc gttaccggct attaccttgt
     3181 gctgtggaag cgcgtcgacg gcaggtggaa gttgcacgta gacattttca acgagaacgg
     3241 ggggcagtag cggaagcttg cccggacaaa gtttcaagac caggatactg acaaattgac
     3301 agtactagcg aagcccgagt caacaacgtt gaggagacgc aaatggctgc atccgtcaaa
     3361 gcgatcccgg acggctacga agccgtcatc ccctacctcg tcgtcgccga cggcgcaggc
     3421 gccatcgatt tctacaagaa ggtttttggc gccaccgaga tcatgagata cgaggacaac
     3481 ggcaaggtcg gccacgcgga gctcaggatc ggccggggaa tcctgatggt ggccgacgag
     3541 taccccgaga tgggctaccg aagtccaaag aagggtgaat tgcctccggc cgggatgatg
     3601 atttatgtcg aggacgtgga caaaacggtc aggcgggccc tcgatgaggg cgccaccgaa
     3661 gagcggccag tcgcggacca gttctacggc gaccgcgctg gcggaatcgt cgatccctac
     3721 ggccaccgct ggtacatcgc gacgcacatc gagaatgtat cgcccgaaga gatggagaag
     3781 cgggcggctg ccaaccgcta gaacgcgagc gtcgatggtg caggagctgc aaatgcacct
     3841 gtactgctcc gggagcgcgg ctgacagact cacgcctccg cggcagcatg caggtttatg
     3901 gaagaacccg cagcgacttc aaggtggtac gagaagcgtc ggctttctct ccctgtgaat
     3961 caggtacagc ccgcttccga cgatcaccgc cgcgcctgcg aaaaccaaag ggccgggcaa
     4021 gtcgccccag atcgcgaggt cgaggagcac gccccagaac aacgtcgagt attcgaaggg
     4081 cgcgacgacg gagacgggcg cagtcttgaa agcttcggtg atcagcagtt ggccgagcgc
     4141 gcctgttacg cccatcccga ccagccacag ggcatcgcta ctgcggacgg gctgccagtc
     4201 cggcagagca agcgcaccgg cgccgactgc gatcatggcg atgaaccaga aagacatggc
     4261 agcggtggag tcggtgcg
//
LOCUS       DASUOM010000460         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1255727,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000460 DASUOM010000000
VERSION     DASUOM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255727"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFY27_09505"
     CDS             complement(<1..208)
                     /locus_tag="VFY27_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765791.1"
                     /translation="MHTMRKQLFVAAACAALAGVALAQVEASSSTSYILDIDPARYSG
                     PAGQRLMLRVGDAPRHVSIGAQDQL"
     gene            complement(252..668)
                     /gene="panD"
                     /locus_tag="VFY27_09510"
     CDS             complement(252..668)
                     /gene="panD"
                     /locus_tag="VFY27_09510"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5765792.1"
                     /translation="MLRSMMIGKLHGLRVTGAELHYEGSIALDPDHTEAAGILPHEFV
                     EIWNKTTGARLSTYVIPGERGSRCCVLNGAAARTCQVGDELIIAARGLVAESGLCDFV
                     SRTVVFCAENRVLHQIEHRMTNNSDGSWKLSAIADS"
     gene            complement(748..861)
                     /locus_tag="VFY27_09515"
     CDS             complement(748..861)
                     /locus_tag="VFY27_09515"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX5765793.1"
                     /translation="MISARELLAGGHRVWIEHATQRYLLQVTRNGKLILTK"
     gene            complement(1041..1472)
                     /locus_tag="VFY27_09520"
     CDS             complement(1041..1472)
                     /locus_tag="VFY27_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5765794.1"
                     /translation="MMWLKAFHIIGVVSWFAGLLYLPRLYVYHAQIQRGVIDDPAGNA
                     RFKVMERKLFLIMTIGAILAAGFGVAMLIVAVDYLLMRWLQVKLALVLVLIGYHGACF
                     ALLQQFADDRNVRTERWYRVFNEVPALLLVAIVILVTVKPF"
     gene            complement(1469..>2155)
                     /gene="hemH"
                     /locus_tag="VFY27_09525"
     CDS             complement(1469..>2155)
                     /gene="hemH"
                     /locus_tag="VFY27_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5765795.1"
                     /translation="TVRESIDSLRAAGVSEIVVVPMFPQYSGATTGAVFDQVASVLRR
                     SRHVPSLRFVSDYHDQPLYIDALAASVREYWREHGDTGHLLMSFHGIPERFVSKGDPY
                     RSQCERTASLLAGRLNLPSQAWSVTFQSRFGRDRWLQPYTIEALAAMPKRGIRSVSVV
                     CPGFAADCLETLEEIGMENRDAFLAAGGETYQYVPALNARTDHVAAILAVLGQRSGER
                     PAGPAPGADP"
BASE COUNT          433 a          668 c          664 g          390 t
ORIGIN      
        1 agagctgatc ctgcgcgccg atgctcacat gtcgcggagc gtcgccgacc cggagcatca
       61 gacgctgacc cgcgggtcct gagtagcgcg cggggtcgat gtcgaggatg tacgaggtgc
      121 tgctgctcgc ttcaacctgg gcgagtgcaa caccggccaa cgcggcacag gcggccgcca
      181 cgaatagttg tttgcgcatg gtgtgcatgg agcactgatt ctataagaag ttccctcagc
      241 atctgttccg actagctgtc ggcgatggct gacaacttcc agcttccatc ggagttgtta
      301 gtcatccgat gttcaatctg gtgcaacacg cgattttctg cacagaaaac gacggtccgc
      361 gacacaaaat cacaaagtcc gctttcggcg acaagaccgc gcgcggcgat gatgagttcg
      421 tcacccacct ggcaggtgcg cgccgcggcg ccattgagca cgcagcaacg cgaaccgcgt
      481 tctcccggaa tcacgtaagt gctcaggcgc gctccggtgg tcttgttcca gatctcgacg
      541 aattcgtgag gcaggatgcc agcggcttcc gtgtggtcgg gatccagtgc gatggatcct
      601 tcatagtgca gttccgcgcc tgtgacacgg agcccgtgca acttgccgat catcatcgaa
      661 cgcaacatcc aagctctccc gcattgagtc gggcgcatgg taatggatgt cgggggcgcg
      721 agtggccgcg cggaggccgg gtgactacta cttggtaagg atcagtttgc cgttgcgcgt
      781 gacctggaga aggtatcgct gcgtcgcatg ttcgatccag acccggtgtc cgccggcgag
      841 caattcacga gcggaaatca cgggctcctg aatgagacct tgggcctctg gcctggcgga
      901 ggttcgcggc agttcgcctg cctggacctt gggacacgcg tcggacatgg gcacctcaaa
      961 caagaacgct tctcatttgc gcccgaagga tatgatgcag cctcgagcaa tgcaagcgcc
     1021 aatgaaatca atggcttact tcaaaacggc ttcacggtca caaggatgac gatggccacc
     1081 agcagcagcg ccggcacttc gttgaacacc cggtaccacc gctcggtgcg cacgttcctg
     1141 tcgtcggcga actgctgcag caacgcgaag caggcaccgt gataaccgat aaggaccagc
     1201 acgagcgcca gcttcacctg cagccagcgc atgagtagat agtcgacggc cacgatcaac
     1261 atggcgacgc cgaatcctgc cgccaggatc gcgccgatgg tcatgatcag gaacagcttg
     1321 cgttccatga ccttgaagcg cgcattgccc gccgggtcgt cgatcacgcc gcgctggatc
     1381 tgtgcgtggt agacgtacag gcgaggcaga tacagcagtc cggcgaacca gctcaccacg
     1441 ccgatgatgt ggaaagcctt gagccacatc atggatccgc tcccggcgcc gggcctgcgg
     1501 gtcgttcgcc gcttcgttgg ccaagcactg ccaatatcgc cgcgacgtga tcggtacgtg
     1561 cattgagcgc cgggacgtac tgataggtct ccccaccggc ggccaggaag gcatccctat
     1621 tctccatgcc tatctcttcg agcgtctcga ggcaatccgc tgcaaagccc ggacacacga
     1681 cactgaccga gcggatacct cgtttcggca tcgccgcgag cgcctcgatg gtgtatggct
     1741 gcagccaacg gtcgcgtccg aatcgcgact ggaaggtgac gctccacgcc tggctcggga
     1801 ggttgagccg gcccgcgagc agtgacgccg tccgttcgca ttgcgagcgg taaggatcgc
     1861 ccttgctcac gaatcgctca ggaattccat ggaacgacat gagcagatgt ccggtgtcgc
     1921 cgtgctcgcg ccagtattcc cgcacgctgg cggcgagcgc atcgatgtac agcggctggt
     1981 cgtggtagtc ggagacgaag cgcagcgacg gcacatgccg cgagcgccgc aacaccgacg
     2041 ccacctggtc gaagaccgca cccgtggtcg cgccggaata ctgcggaaac atcggcacca
     2101 cgacgatttc cgagacgccg gccgcgcgca gcgaatcgat ggattcccta acggt
//
LOCUS       DASUOM010000461         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_375381,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000461 DASUOM010000000
VERSION     DASUOM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375381"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFY27_09530"
     CDS             complement(<1..383)
                     /locus_tag="VFY27_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765796.1"
                     /translation="MNRPAHLLACAFLFGCAAQPATGPTNATDSDAVSILLFGDTGYD
                     YDWLEEDDYEDAFTGREFVINELDDWVEDHRPIEEFAIPPMHFAEQTGGYVMASGMWP
                     VSKAVHTWCAADDKCDFGVMLGDNI"
     gene            complement(380..1297)
                     /locus_tag="VFY27_09535"
     CDS             complement(380..1297)
                     /locus_tag="VFY27_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5765797.1"
                     /translation="MRTYDLVTIGNYTKDTIVSAAGTRHVDGGGYSYAAHAAHLKGIS
                     IAAVTRLAAGDRASTDALRAKGIDVHIIESPRSTLMRLEYPTHNVDERTLTVAAVADP
                     FTPELLRDFQARAFVVNGSARGEASLEVMQALKAKGGLLCADLQGFIRVVSDTGQLEY
                     RPWPEQAEILGLVDILKTDAVEAEFITGKDDIHKAAAELATFGPEDIVLTHRDGLLVL
                     SAGKHYEAGFHPEELRGRSGRGDTCMGSYVSSRLDASAEESTVWAAAVTSLKLEREGP
                     VNRSRADVEALIAAKYGSGKARTAAGSIA"
     gene            complement(1321..2571)
                     /locus_tag="VFY27_09540"
     CDS             complement(1321..2571)
                     /locus_tag="VFY27_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5765798.1"
                     /translation="MRVLVAITLGYGLVYTCRLALSVVKKPLIDEGIFTPVELGFIGA
                     GLFYGYAVGKLVNGFLADHSNMKVFFAFGVLLSAIANLGMGFSTVLAVSVLLWAFNGW
                     FQGFGAPAGIVSLANWYSNSERGRFYGIWSTAHSIGEGLTFVAVAALVAHFGWRYGFW
                     GPGLLCVAVAFGLYYFMQDRPRTMGLPEVNDWHNDHWEKDELHEKPSLLKVQFSILAL
                     PAIWILALASATNYIVRYAINSWGILYLQEARGYSLVEAGSLLMANTIAGIVGCIAFG
                     YLSDKVFNARRPPANLLFAILEIVALALIFFGPQNTPMLLFAFILYGIGLNGLVTSLG
                     GLFGVDIAPKRAAGAIMGFVGIFSYIGAGIQENVSGYLIQKGTTMVGDTRIYDFSDVI
                     YFWLGASVVSMLLAATLWRVKLRD"
BASE COUNT          573 a          870 c          848 g          526 t
ORIGIN      
        1 tagatgttgt cgccgagcat taccccgaaa tcgcacttgt catccgctgc acaccacgtg
       61 tgcaccgcct tcgataccgg ccacattcca ctcgccatca catagccgcc ggtctgctcc
      121 gcaaaatgca ttggcgggat cgcgaattcc tcgatcggcc ggtggtcctc gacccagtcg
      181 tcgagttcgt tgatgacgaa ttcgcgcccc gtgaaggcgt cttcataatc gtcctcctcg
      241 agccagtcgt agtcgtaacc ggtatcgcca aaaaggagaa tgctgacagc gtcgctatcc
      301 gtggcattcg tcggacccgt cgcgggctgt gcggcacagc cgaacaggaa cgcgcaggcg
      361 agcaagtgtg caggacgatt catgcgatgc tcccggccgc cgtgcgcgct ttgccgctac
      421 catacttcgc ggcgatcagc gcctcgacgt ctgcccggga gcggttcacc ggcccttcgc
      481 gttcgagctt gaggctcgtc acggccgcgg cccagacggt ggattcctct gccgatgcat
      541 cgagacggct ggaaacgtag gaccccatgc aggtgtcgcc acggccactg cgtccgcgaa
      601 gttcttccgg atggaagccg gcctcgtagt gttttccagc cgacagcacg agcagaccgt
      661 cgcggtgcgt caacacgata tcttcgggac cgaaggtcgc aagttccgcg gcggccttgt
      721 gaatatcatc cttgccggtg atgaattccg cttcgactgc gtcggttttc aggatgtcga
      781 caaggccgag gatttccgcc tgctccggcc acggccgata ttcgagttgc ccggtgtcgc
      841 tcacgaccct gatgaaccct tgcaggtccg cgcacaacag cccgcctttc gccttgaggg
      901 cctgcatgac ttcgagcgac gcttccccgc gggcggagcc gttcaccacg aatgcacgag
      961 cctggaaatc gcgaagcaat tccggcgtga aagggtcggc gacggcggcg accgtcaatg
     1021 ttcgttcgtc cacgttgtgt gtagggtact ccaggcgcat cagggtggag cggggcgatt
     1081 cgatgatgtg cacatcgata cccttcgccc gcagcgcatc ggtagaggcg cgatccccgg
     1141 ccgccagccg cgtcaccgct gcgattgaga taccttttaa atgggccgca tgcgcggcgt
     1201 agctgtagcc gccgccgtcc acgtggcgcg tgcccgcggc cgacacgatg gtgtccttgg
     1261 tatagttgcc gatggtgaca aggtcgtaag ttctcaaagc ggactcccgt tgaaggttga
     1321 tcagtcgcgc agcttgacgc gccagagtgt tgcggccagc agcatcgaga cgacggatgc
     1381 gccgagccag aagtagatga catcggaaaa gtcatagatg cgcgtgtcgc caaccatcgt
     1441 cgtacctttc tggatgagat aaccgctgac attctcctgg atgcccgcgc cgatatagct
     1501 gaaaatcccc acgaatccca tgatcgcgcc ggcagcacgc ttcggtgcga tgtccacgcc
     1561 gaacagcccg ccgagcgacg tcaccaggcc gttcagcccg atgccgtaga gaatgaaggc
     1621 gaacagcagc atcggcgtgt tctgcggtcc gaagaaaatc agtgcgagtg cgacgatttc
     1681 gaggatggca aacaggaggt tcgccggcgg ccggcgcgcg ttgaacacct tgtcggagag
     1741 gtagccgaag gcgatgcagc cgacgatgcc ggcaatcgtg tttgccatca gcaaactccc
     1801 ggcctcgacc agcgagtagc ctcgcgcttc ctgcaggtaa aggatgcccc agctgttgat
     1861 ggcgtaacga acgatgtagt tcgtggcgct cgccagcgcg agaatccaga tggccggcag
     1921 tgcgaggatc gagaactgca ccttgagcag gcttggtttc tcgtgaagtt cgtctttctc
     1981 ccaatggtcg ttgtgccagt cgttgacttc cggcaggccc atcgttcgtg ggcggtcctg
     2041 catgaagtag taaagcccga aagcaaccgc gacgcacagg agtcccggac cccagaaccc
     2101 gtagcgccag ccgaaatgcg cgaccagcgc ggcgacggcg acgaacgtga gcccttcgcc
     2161 gatcgaatgc gcagtactcc agattccata gaagcgcccg cgctcgctgt tgctgtacca
     2221 gttcgcgagc gagacgatgc ccgccggcgc gccgaagccc tggaaccagc cgttgaatgc
     2281 ccacagcaac accgagacag cgagtaccgt ggagaatccc atgccgagat tcgcgatcgc
     2341 cgagagcagc acgccgaacg caaagaacac tttcatgttc gagtggtcgg cgaggaagcc
     2401 gtttaccagc ttgccgacgg cataaccgta gaacagcccc gcaccgatga agccgagttc
     2461 gacaggggtg aagataccct cgtcgatcag cggctttttc acgaccgaca aggcgagcct
     2521 gcaggtgtac acgagaccgt aacccagggt gatcgcgacc agcacgcgca ttcggtgccg
     2581 ccggtactgg cgatcgattg cgtcgcggtc ggaaagaaga ggccggtccg cgccagttcg
     2641 aaagtaattc aggaatgtct gcaaaacgta acgagccccc tttcctgtta acgtcggccg
     2701 ttcaacgtaa cagtcatcgc ggctttcccc gccgcgccca cgcattgaaa ttttcgttcg
     2761 attataatag aaaatagaat agacgttcca gcttggttcc tgccgcccgg caggaga
//
LOCUS       DASUOM010000462         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_536244,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000462 DASUOM010000000
VERSION     DASUOM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536244"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFY27_09545"
     CDS             <1..974
                     /locus_tag="VFY27_09545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5765799.1"
                     /translation="EAEVDKIFAAYQGEETPGAVVRVIKDGKPVLTRSFGFANVEEKI
                     PVTPETNFRLASVTKQFTAMCILMLVDRGELHLTDTLTDVLPDFPEYGSGITITHLLQ
                     HTSGLKDYEPFVPEDSPVQVVDAGVLDLMRQQVSVDFEPGSEHRYSNSGYAVLAMVVE
                     QVSGMPFPQFLEQNIFEPLGMVNTVAHQDGISTVSSRAFGYHVTEGGVEYSDQSPWSA
                     VLGDGGIYTSVVDLTLWDAALYGDELVSAEFMQQAFTPHLEDYGFGWRIDEYNGHRRM
                     HHSGSTVGFRNFIQRFPDERLTVIALTNRRDPEVDPLAERVADLFLE"
     gene            1146..1811
                     /locus_tag="VFY27_09550"
     CDS             1146..1811
                     /locus_tag="VFY27_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5765800.1"
                     /translation="MKASIAASKALTVAAAVAGLLLASVVHAQDRDTGELPRFALTPY
                     GGYTFGGEFQDDEENLAVEVDDAPHFGLIFNIRETANTQWEIFYARQGAEADTSAISP
                     SQPEVDIDVQYLHLGGTYVADGELARPFLAAGIGGTRFDPGPLTFDSENFLSFGVGAG
                     WQLQPTDRLGVRLEGRVLGTFLNSDTALFCETGVDENVCAIAASGDLYWQFQTSLGVV
                     LQF"
     gene            complement(1967..2977)
                     /locus_tag="VFY27_09555"
     CDS             complement(1967..2977)
                     /locus_tag="VFY27_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX5765801.1"
                     /translation="MQIATPYLVFLGAVETPRDAKTGQGLVDWRRESCLAQLRYPGCA
                     ADLGLPDMTPAEAYAEGARSLVIGVASFGGRFEPSWIDTFDQAMRAGLDIVSGMHTRL
                     ASVGRLAAVAAETGRRLVDVRVPPAAIPIGSGRRRSGNRVLMVGTDCCVGKKYTALAM
                     HRELLSRNCKATFRATGQTGIMIAGGGIPMDAVVSDFLAGAAELLSPDNDEDHWDVIE
                     GQGSLFHPAYAAVTLGLLHGSQPDVLILCHDASRTHIDGYSDYAIPALADCIDGYLPV
                     ARLTNKRVRFAGVSLNTAGLRESARRAAVAHTEKETGLPVADPLAAGAGALVDAVLAA
                     AR"
     gene            complement(2983..4548)
                     /locus_tag="VFY27_09560"
     CDS             complement(2983..4548)
                     /locus_tag="VFY27_09560"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5765802.1"
                     /translation="MGLLSRTVTAVALLAMSACAPEVPEAGNNTPALLVHNVIIVDGS
                     GAPARAGSVRIADGTITAVGDLQADKGETLFDGHGMVLAPGFIDTHSHADRDLLEQPD
                     ALPAVSQGITTVVVGQDGGSELPLSGFFADVDATPLAVNIASYAGHNTLRSAVLGKDY
                     RRHATTDEVDAMAALLEQELASGALGLSSGLEYDPGIWSEPSEVLALAKVAAAHGGRY
                     ISHVRSEDRWFEKALDEIINIGRVTGMPVQVSHIKLAMKRLWGETSRIIATLDAARAE
                     GVQISADLYPYEYWQSNLMVLLPERDYTDRAAIEEALDQIAPPDGLWMTRFDPQPEYV
                     GKTLTEIAALRNTDPAAAFMHLAEASAQMEEGTGEGADAIIGTSMREDDIRKMLLWAE
                     TNICTDGAFEDLHPRARGAFTRVLGRYVREEGLLSLEEAVHKMTGLAAAHMDFRNRGI
                     IREGMRADLVLFDPETVIDRATSQEPELLSVGIEAVWVGGELVFEDGRPTGALPGQIL
                     RRAQRPEQEPLPQ"
BASE COUNT          850 a         1514 c         1516 g          906 t
ORIGIN      
        1 acgaagcgga agtagacaag atcttcgccg cgtaccaggg ggaggagact ccgggcgccg
       61 tggtgcgagt gataaaggac ggcaagcccg tgctgacgcg aagctttggg ttcgcaaatg
      121 tcgaggagaa gatcccggtg acgccggaga cgaatttccg gctggcgtcg gtgacgaagc
      181 agttcacggc gatgtgcata ttgatgctgg tggatcgcgg ggaactgcac cttaccgata
      241 cgcttacgga cgtgctcccg gactttccgg agtacggcag cggcatcacg atcacgcacc
      301 tgttgcaaca cacttccggg ctcaaggact acgaaccgtt cgtgcccgag gattcgccgg
      361 tgcaggtggt ggatgcgggg gtgctggacc tgatgcggca acaggtgagc gtcgacttcg
      421 agccgggcag cgaacatcgc tacagcaatt ccgggtatgc ggtgctggcg atggtcgttg
      481 aacaggtgtc cggtatgcca ttcccgcagt tcctcgagca gaacatcttc gagccgctgg
      541 gaatggtcaa tacagtggcg caccaggacg gaatatcgac ggtttccagc cgtgctttcg
      601 gttatcacgt gaccgagggc ggcgtggagt atagcgacca gagcccgtgg agcgcggtgc
      661 ttggagacgg cgggatctac acgtccgtcg tcgacctgac attgtgggac gcggcgcttt
      721 acggcgatga gctggtcagc gcagagttca tgcagcaggc gttcacgccg catctcgaag
      781 actacggctt tggctggcgc atcgatgagt acaacgggca caggcgcatg caccacagcg
      841 gcagcaccgt cggcttccgc aatttcatcc agcgctttcc cgatgagcgc cttaccgtga
      901 ttgccttgac caaccggcgc gatccggagg tcgatccgct ggcggaaagg gttgccgatc
      961 tcttcctcga gtaagaatgc gtcgttccag ctccgggctt ggctcaacgg ggatcgatag
     1021 cattttgatt tatgcggctg cgctgtgcgt ggtctagaat ggaaacacgg gattcgcgga
     1081 tcggcaggcg ccggcttata gccgccgcga atcgcttcac gttccggcaa ggaggcagga
     1141 ccagaatgaa agcatcaatc gctgcatcga aagctctaac ggtagctgcg gccgtcgcgg
     1201 gtctcctgct ggcgagcgtg gtgcacgccc aggatcgtga caccggtgaa cttccccgct
     1261 tcgcgctgac accctacggc ggatacacct tcggcggcga gttccaggac gacgaagaga
     1321 atttggccgt ggaagtggac gatgcgccgc atttcggcct gatcttcaac attcgcgaga
     1381 cggccaacac ccagtgggag attttctacg cccgccaggg ggcggaggcg gatacgtccg
     1441 cgatatcgcc atcccagccg gaggtggaca tcgatgtgca gtacctgcat ctcggcggca
     1501 cgtacgtcgc ggacggagag ttggcgaggc cctttttggc ggcgggcatc ggcggcaccc
     1561 gcttcgaccc cggtcccctg acgttcgata gcgagaattt cttgtccttc ggcgtgggtg
     1621 cgggctggca actgcagccg acggaccggc tgggggtgcg gctggaaggc cgggtgctgg
     1681 gaactttcct caattcggac accgcgctgt tctgcgaaac gggcgtcgac gagaacgtct
     1741 gcgccatcgc cgccagcggc gatctctact ggcaattcca gacctcgcta ggcgtggttc
     1801 tgcagttttg accaatttgt cgcccgtgag actggctcct gtagcgctcg aattgcccag
     1861 ctcaagacac ggctggaaca gaccgctgca ggagcccgcc acgcgggcga gcgcgataac
     1921 ccggagtcac tcgccgccat gagctggccc cggcgactcc gacactctac cgtgccgccg
     1981 caagaaccgc gtcgaccagc gcacctgccc ccgccgccag cgggtccgcg accggcaagc
     2041 ctgtttcctt ctccgtatgc gcaaccgcag cgcgacgcgc ggactcgcgc agtcccgccg
     2101 tattcaggct gacgccggca aaccgtaccc ttttattcgt caggcgtgcg accggcaggt
     2161 agccatcgat acagtccgcc agggccggga tcgcataatc ggaatagccg tcgatgtgtg
     2221 tccggctcgc gtcgtggcac aggatcagta catccggttg cgatccgtgt agaagcccga
     2281 gcgtcaccgc tgcatacgcc gggtggaaaa gcgacccctg accttcgatg acgtcccagt
     2341 gatcctcgtc attgtcgggt gacaagagct cggcggcgcc tgcgaggaaa tcggagacga
     2401 ccgcatccat cggaatgcca ccgccggcaa tcatgatccc ggtctgcccc gttgcacgga
     2461 acgtggcttt gcaattccgc gacagcagct cacggtgcat tgcaagcgcc gtgtatttct
     2521 tgccgacgca gcagtcggta cccaccatca gcacgcggtt tccgctgcgg cgcctgccgc
     2581 tgccgatggg gattgcggcg ggtggcaccc gcacgtcgac cagcctgcgc cccgtttccg
     2641 ccgcgaccgc ggcgagcctg cccacagagg cgaggcgcgt gtgcatgccg ctcacgatgt
     2701 cgaggcccgc gcgcatcgct tgatcgaacg tatcgatcca cgacggctcg aagcgtccgc
     2761 cgaaggacgc gactccgatc acgaggcttc gcgctccttc cgcgtaagcc tcggcgggcg
     2821 tcatgtccgg caagcccaga tcggcggcgc aaccgggata tcgcaactgc gcaaggcagg
     2881 actcgcgccg ccagtccacc agcccctggc cggtctttgc gtcacgcggg gtctcgacgg
     2941 cgccgaggaa aacaaggtaa ggggtggcga tttgcatgat gctcactgcg gcaggggttc
     3001 ttgctcaggt cgctgcgcgc gtcgcaggat ttggccaggc aatgcgcctg tcggacgacc
     3061 atcttcgaaa accagttcgc cgcccaccca gacagcttcg atgccgactg atagcaactc
     3121 cggctcctgc gaggttgcac ggtcgatgac ggtttccgga tcgaacagca cgaggtccgc
     3181 ccgcatcccc tcgcggatga ttcccctgtt gcggaagtcc atgtgagcgg ccgcgagccc
     3241 ggtcattttg tgcacggctt cctccagcga cagcagcccc tcctcacgca cgtagcggcc
     3301 cagcacccgg gtgaaggcgc cccgtgcgcg cggatgcagg tcctcgaacg ccccgtcggt
     3361 gcagatattc gtttcggccc acagcaacat cttgcgtatg tcatcctcgc gcatgctggt
     3421 ccctatgatg gcatcggcgc cttccccggt cccttcttcc atctgcgccg aagcttccgc
     3481 gaggtgcatg aacgccgcgg ccgggtcggt attccggagc gctgcaatct ccgtcagcgt
     3541 cttgccgaca tactccggct ggggatcgaa acgcgtcatc cagaggccat ccggcggggc
     3601 tatctggtcc agtgcttcct cgatggctgc acggtcggtg tagtcgcgtt ccggcagcag
     3661 caccatgagg ttcgactgcc agtattcgta gggatagagg tccgccgaga tctgcacacc
     3721 ttcggcccgc gctgcatcga gcgtcgcgat gatgcgcgac gtttctcccc acagccgctt
     3781 catggccagc ttgatgtgcg atacctggac cggcatgccg gtaacacgtc cgatgttgat
     3841 gatttcatcc agtgctttct cgaaccaccg gtcctcgctg cgcacatggc tgatgtaacg
     3901 tccgccgtgc gccgccgcca ctttagcgag cgccagcacc tcggaaggct ccgaccagat
     3961 gcccgggtcg tactcgagcc cgctggagag cccgagtgcg ccgctcgcca gttcctgctc
     4021 gaggagggcc gccatcgcgt cgacttcatc cgtggttgcg tggcgtctgt agtctttgcc
     4081 gagtaccgca gagcgcagcg tattgtgacc cgcgtaggag gcgatattga cggcgagcgg
     4141 ggtggcgtcc acgtcggcga agaatcctga aagcggcaac tccgagccac cgtcctggcc
     4201 gacaacgacg gtggtgatgc cctggctgac tgctgggagt gcgtccggtt gttcgagcag
     4261 gtcccggtcg gcatgactgt gggtgtcgat gaaccctggc gccagtacca ttccgtgccc
     4321 atcgaaaagt gtctcgccct tgtccgcctg caggtcaccg accgcagtga tggtaccgtc
     4381 ggcgatgcgc acgctgcccg cacgtgctgg cgcacctgag ccatccacta tgatcacgtt
     4441 gtgaacgagc agggccgggg tattgttgcc tgcttccgga acttcgggag cgcacgcgct
     4501 catcgccagc aaggcgactg ctgtaacggt cctgctgagt agtcccactg ctctcttcct
     4561 tcttacggct gctgaccggt tcgatgtgcc gcgaccggga aaacaacata gccctccacc
     4621 ggtggcattg gagcacgcga tccgaggcag ggagcctgaa attcacaata tgcaaatccg
     4681 ctgttccggc acatgcatcg acggtacggt cgagcgcgga ggacctacaa tgtcaagaat
     4741 cctgcgtgct gccctgctcg tttccttcgc aatgtccttt cccgtg
//
LOCUS       DASUOM010000463         8298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_898015,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000463 DASUOM010000000
VERSION     DASUOM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898015"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="paaX"
                     /locus_tag="VFY27_09565"
     CDS             <1..1041
                     /gene="paaX"
                     /locus_tag="VFY27_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HEX5765803.1"
                     /translation="SITFPSTPFDPGAKETMSLESVTRSLIEEFRSRPTLRAGSLIVT
                     VFGDAIAPRGGSVWIGSLIRVMADFGISERLVRTSVFRLARDGWLDVEQLGRRSYYRL
                     TEEGARRFAQATHRIYGEPRQSWDGNWILVLTACLPAEKRELVRRELSWLGFAGIGND
                     VLAHPSPDPVELDAQLKQIDAGELVVMHGRGAGPAAQNGAIRALVRKSWNLEDIEERY
                     KAFLGRFRPVFAASRRSKRVPPRIAFQVRTLMLQEYRKILLRDPLLPAELLPQTWHGV
                     PAYQLCRNLYRKVCNAADEYLGEAMETAEGPLPPPSPEFFRRFGGLDAANGKILKQAT
                     QARSTSEMRARE"
     gene            1038..1478
                     /gene="paaI"
                     /locus_tag="VFY27_09570"
     CDS             1038..1478
                     /gene="paaI"
                     /locus_tag="VFY27_09570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX5765804.1"
                     /translation="MSSPEVARRCADEMFKKDRASRELGIVVEVGTAGSASARMEVTE
                     RMINGLGVCHGGYIFTLADTAFAFACNGYDQVTFAAGASIQFIRSARLGDALLAEARE
                     QHRGRSSGIYDVAVSNQEGKLVALFRGQSRATGAPPVPAAPDHL"
     gene            1547..2575
                     /gene="paaA"
                     /locus_tag="VFY27_09575"
     CDS             1547..2575
                     /gene="paaA"
                     /locus_tag="VFY27_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX5765805.1"
                     /translation="MYTQGLDQPGASEAAAETEPQLKRFQSRIDAEEKIEPKDWMPEA
                     YRKTLVRQISQHAHSEIIGMQPEGNWLTRAPTLRRKAILLAKVQDEAGHGLYLYSAAE
                     TLGTSRDEMVDGLLSGKAKYSSIFNYPTLTWADVGAIGWLVDGAAIMNQIPICRCSYG
                     PYARAMVRICKEESFHQRQGFEIMLELCRGNSEQKDMAQDALNRWWWPSVMMFGPSDS
                     ESKHSAQSMAWKIKRFTNDELRQKFIDITAPQAEFLGLIIPDDKLEYNAATGHYRFGE
                     IDWNEFYEVLAGNGPCNRQRLRHRRKAQEDGAWVRAAAHAYAKKHHDVAGRPLAEARS
                     AAGGQGNG"
     gene            2568..2855
                     /gene="paaB"
                     /locus_tag="VFY27_09580"
     CDS             2568..2855
                     /gene="paaB"
                     /locus_tag="VFY27_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX5765806.1"
                     /translation="MADETWPLYEVFIRARSGLSHRHAGSVHAADDQMALDHARDVYT
                     RRNEGVSIWVVRSSEIVASDPAESAAFFDPAADKIYRHPTFYEIPDGVENL"
     gene            2873..3631
                     /gene="paaC"
                     /locus_tag="VFY27_09585"
     CDS             2873..3631
                     /gene="paaC"
                     /locus_tag="VFY27_09585"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX5765807.1"
                     /translation="MDSNGARFNYVLRLGDNALVLGQRLSELVTRAPELEEELATANF
                     ALDYIGQARLFYSYAGELEARGRSEDDFAYLRDERDFRNLLLVEQPNGHFGDTIVRQF
                     LFESFYVLQLGALAGCSEPRVAEIAARAVKEVRYHLRHVSQWLVRLGDGTAESHAHVQ
                     QSLDDQWRFTGEMFAGDEVDETFRQEWNGPHLEQLEADWNSRVDAVLAEATLARPPAQ
                     WMDGGGRQGRHSEHLGYLVAEMQFLQRAYPGARW"
     gene            3650..4153
                     /gene="paaD"
                     /locus_tag="VFY27_09590"
     CDS             3650..4153
                     /gene="paaD"
                     /locus_tag="VFY27_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX5765808.1"
                     /translation="MSMSQPVESLTLEDIRTLLHAVADPEIPVLSIMDLGIVRDVKLD
                     ADGVEVQVSPTYTGCPATEVIEQMIVAKLKGNGVERVTIKRVLSPPWTTEWLSETGRQ
                     KLRKYGIAPPERGAGKRSLFGAAIVHCPRCSTERTTRVSEFGSTPCKASYKCDECLEP
                     FEYFKCI"
     gene            4189..5280
                     /locus_tag="VFY27_09595"
     CDS             4189..5280
                     /locus_tag="VFY27_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5765809.1"
                     /translation="MRRFHSLTVQSVTPEAEDAMRISLQVPEELREQFRFLPGQHLPL
                     QVVLDGRKIRRTYSICSVPGEWPLEIGVRVQPGGLFSGFVTSGLSAGSTLEVMPPFGQ
                     FHVTCDPVAVTAARGKFRVAFAAGSGITPILSIMRTTLQNEPGSRFLLFYGNRRQRTT
                     MFIEELYALKNCYPDRLQLNFVFSREEQEFPLMSGRLDGPKVRELWKAFVRQNPDESF
                     VCGPDTMIDTVSEALVELGMESSAVHVERYGVPRRPTAQPPPAPAVARGADHLASVTV
                     VMDGHEKTFDMSADSSNIVDAAAEKGVELPYSCKAGVCATCRTWLREGKVRMHANYGL
                     EPWEVKKGFVLACQSVPMTDTVVLDYDKV"
     gene            5597..6163
                     /gene="fabA"
                     /locus_tag="VFY27_09600"
     CDS             5597..6163
                     /gene="fabA"
                     /locus_tag="VFY27_09600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX5765810.1"
                     /translation="MSETGKKSAVTGLADLPPDRKNSYSREELLQCARGELFDPDSAK
                     LPLPDMLMTDRVPLIASSGGQYGKGEIIAELDIDPDMWFFKCHFAGDPVMPGCLGLDA
                     LWQLVGFFLVWHGFKGRGRALGVGNVKFSGQILPTSRLVTYNLQMKRLITRKLILAIA
                     DGTVSVDGRQIYTAEDLRVGLFTSTDNF"
     gene            6252..7472
                     /gene="fabB"
                     /locus_tag="VFY27_09605"
     CDS             6252..7472
                     /gene="fabB"
                     /locus_tag="VFY27_09605"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX5765811.1"
                     /translation="MRRVAITGLGVVSCLGNNQEEVLESLKAGRSGITFNEKFHEMGL
                     RSQVCGSVALDISGHIDRKVRRFMGDAAAYSYIAMQQAIEDASLEDTDVSNPRTGLIA
                     ASGGASSSNIVLSADTLRQKGVRKIGPYMVPRTMGSTVSACLATPFKIKGVNYSITSA
                     CATSAHCIGAAMEQIQFGKQDIVFAGGGEEEDWTLASLFDAMGALSSNYNDAPGTASR
                     PYDQNRDGFVIAAGAGMVVVEELEHARARGAKIYAEIVGYGANSDGFDMVAPSGEGAV
                     RCMQLARSTVNAPVDYINTHGTSTPVGDTRELQALREVFGNEIPRFASSKSLGGHSLG
                     ATGVQESIHCLLMLQDDFIAPSINVFDIDPAAEGMPLVTQRVDSAGLRTVMTNSFGFG
                     GTNSSLIFQRLDEK"
     gene            7484..>8298
                     /gene="fghA"
                     /locus_tag="VFY27_09610"
     CDS             7484..>8298
                     /gene="fghA"
                     /locus_tag="VFY27_09610"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX5765812.1"
                     /translation="MEKLSEHRAFEGTVGFYRHRSDVNNCDMQFSVFVPPGAANAPAP
                     SLTWLSGLTCTEETFMIKAGAQRIAAELGLMLIAPDTSPRGKNVPDDPDGAYDFGLGA
                     GFYVNATQPPWNRHYHMYDYVTKELPDLVFAEFPGDRSRQGIFGHSMGGHGALTIGLK
                     NPATYTSISAFAPIASPVRCPWGEKALGNYLGSSVEHRKSWLEYDATELVKSIGQAGG
                     ISKPVNEILVDQGLADQFLQEQLHPHLLEAACREQGVALKMARHEGYDHGYYF"
BASE COUNT         1621 a         2505 c         2688 g         1484 t
ORIGIN      
        1 agtatcacat tcccgtcaac gccgttcgat cctggcgcaa aggaaaccat gtctctcgag
       61 tcagtcacgc gctcgctcat cgaggagttt cgctccaggc cgacgctgcg ggcgggatcg
      121 ctgatcgtga ccgtcttcgg cgatgcgatc gcaccgcgcg gcggcagcgt gtggatcggc
      181 agcctgatcc gcgtcatggc ggacttcggc atcagtgagc gactggtccg gacgtcggtg
      241 ttccggctgg cgcgtgacgg ctggctcgac gtcgagcagc tcggccggcg aagctattac
      301 aggctgacgg aggagggggc gcggcgtttc gcgcaggcga cgcaccggat ctacggcgag
      361 ccgcggcaga gctgggatgg caactggatc ctggtgctga ccgcctgcct gcctgcggaa
      421 aagcgggaac tggtccggcg cgagctttcc tggctcggtt ttgccggtat cggtaacgac
      481 gttctcgcgc acccgtcgcc ggatccagtg gagctcgatg cccaattgaa gcagatcgat
      541 gccggcgagc tggtcgtcat gcacgggcgt ggcgctggtc ccgcagcgca gaacggtgcc
      601 atacgggcac tcgttcgcaa aagttggaat ctcgaggaca tagaggagcg gtacaaggct
      661 ttcctcggcc ggttccggcc ggtgttcgcg gcgagcaggc ggagcaaacg ggtgccgccg
      721 cggatcgcat tccaggtccg cacgctgatg ctgcaggaat accgcaagat cctcctgcgc
      781 gacccgctgt tgcccgcgga actgctgccg caaacgtggc acggcgtgcc ggcttaccag
      841 ctatgccgca acctctaccg gaaggtctgc aatgcggcgg acgaatacct gggtgaggcg
      901 atggagaccg cggagggacc gctacccccg ccctcgcctg agttcttcag aaggttcggc
      961 ggactggatg ctgcgaacgg gaaaattctg aaacaggcca cgcaggccag gagcacaagt
     1021 gaaatgagag caagagagtg agtagccccg aggtggcgcg tcgttgcgcg gacgaaatgt
     1081 tcaagaaaga ccgcgcatcg cgcgaacttg gcatcgtggt cgaggtaggg acggcgggta
     1141 gcgcaagtgc ccgtatggaa gtgacggaga ggatgatcaa cggcttgggc gtctgtcacg
     1201 gcggctatat cttcacactg gcggacaccg cgttcgcctt cgcctgcaac ggctatgacc
     1261 aggtgacgtt cgccgccggc gccagcatcc agttcattcg ctccgcgcgg ctgggagatg
     1321 cgctcctggc ggaggcccgc gagcagcacc gcgggcgcag cagcggaatc tacgacgtgg
     1381 cggtcagcaa ccaggaggga aagctcgtgg cgctgtttcg cggccagtcc cgcgccaccg
     1441 gggcccctcc ggttcccgcg gcaccggacc atttatgaca cagaaaacgt tgaatttgtc
     1501 cgaaaagcgt gacacattgg acgcttgaaa accggagctt caggacatgt acacccaggg
     1561 cctcgaccag ccgggagcga gtgaggcggc ggcggaaacg gaaccgcagc tcaagcgttt
     1621 ccagtcgcgt attgacgcgg aagagaaaat agagccaaag gactggatgc ccgaggcgta
     1681 ccgcaagacg ctggtgaggc agatctccca gcacgcgcat tcggaaatca tcggcatgca
     1741 gccggagggc aactggctga cgcgcgcgcc cacgctgcgc cgcaaggcca ttttgctcgc
     1801 gaaagtgcag gatgaagcag ggcacggcct ctatctttac agtgcagcgg agacgctcgg
     1861 cacttcgcgg gacgaaatgg tcgatggact gctgagcggc aaggcgaagt actcgagcat
     1921 cttcaactat ccgacgctga cgtgggcgga cgtcggcgcc atcggctggc tggtagacgg
     1981 cgcagccatc atgaaccaga taccgatctg ccgctgttcc tacggtccct atgcgcgcgc
     2041 catggtgcgg atctgcaagg aagagagttt tcaccagcgc cagggcttcg agatcatgct
     2101 cgaactgtgc cggggcaaca gcgagcagaa ggacatggcg caggatgccc tcaaccggtg
     2161 gtggtggcct tccgtcatga tgttcggccc gagcgacagc gaatcgaaac attcggcaca
     2221 gtcgatggcc tggaagatca agcggttcac gaatgacgaa ctccggcaga agttcatcga
     2281 tatcaccgcg ccccaggcgg agttcctcgg gctgataatt ccggacgaca agctcgagta
     2341 caacgcggca acgggtcact accgattcgg cgagatcgac tggaacgagt tctacgaagt
     2401 gcttgccggc aacggcccct gcaatcgcca gcgcctgcgc caccggcgga aggcccagga
     2461 ggacggagcc tgggtacgtg ccgcggcgca cgcctatgcg aagaagcacc atgatgtcgc
     2521 gggccgaccg cttgccgaag cacgctcagc agccggaggg cagggcaatg gctgacgaaa
     2581 cctggccgct ctacgaagtt ttcatccggg cgcgctccgg tctgagccac cggcacgctg
     2641 gcagcgtgca tgcggcggac gaccagatgg ctctggacca tgcgcgcgat gtctatacgc
     2701 ggcgcaacga gggcgtcagc atctgggtcg tgcgctcgag cgaaatcgtc gcgtccgacc
     2761 ctgccgagtc tgccgcgttc ttcgatcctg cggcggacaa aatttaccgg cacccgactt
     2821 tttacgaaat tcccgatggg gtagagaacc tttagttgcc atggacgatg ccatggacag
     2881 taacggggca cgcttcaact acgtcctgcg cctgggcgac aacgcgctgg tgctgggaca
     2941 gcggttatcg gagctggtga cacgcgctcc cgaactggaa gaggaactcg ccaccgcgaa
     3001 cttcgcgctc gactacatcg gccaggcacg gcttttctac agctacgccg gcgaactgga
     3061 ggccaggggc cggagcgaag acgatttcgc gtacctgcgg gatgagcgcg atttccgcaa
     3121 cctgctgctg gtcgagcagc cgaacgggca tttcggcgac acgatcgtcc ggcagttcct
     3181 gttcgagtct ttctacgtgt tgcagctggg agcactggcc ggctgcagcg aacctcgcgt
     3241 ggccgagatc gcggcacgtg cagtcaagga agtccgctac cacctgcgcc acgtttcgca
     3301 gtggctggtg cgcctcggcg acggcacggc tgaaagtcat gcccatgtac agcaatcgct
     3361 ggacgaccag tggcgcttca ccggggaaat gttcgctggt gacgaggtcg acgaaacctt
     3421 caggcaggag tggaacgggc cgcacctgga gcagctcgaa gccgactgga acagcagggt
     3481 cgatgccgta ctcgccgagg ccacgctcgc tcgtccgccc gcgcagtgga tggatggcgg
     3541 cgggcgccag ggcaggcaca gcgagcacct ggggtatctc gtggccgaga tgcagttcct
     3601 gcagcgagcg tatcccggtg cccgttggta acagggccgg ggtgagaaca tgagcatgtc
     3661 gcagccggtc gaatccctga cgctcgagga catccgtacg cttctgcatg cggtggccga
     3721 ccccgagatc ccggtactca gcatcatgga cctgggcata gtgcgggacg taaagcttga
     3781 cgccgacggt gtcgaggtcc aggtgtcgcc gacctatacc ggttgtccgg cgacagaggt
     3841 gatcgagcag atgattgtcg cgaagctcaa aggcaatggc gttgagcgcg tgacgatcaa
     3901 gcgcgtgctg tcgcctccct ggaccaccga atggttgagc gagacgggcc ggcagaagct
     3961 gcgcaagtac gggattgcgc ctccggagcg cggcgctggc aaacgcagcc tgttcggggc
     4021 ggccattgtc cactgtcccc gttgttcgac ggagcggaca acgcgagtca gcgagttcgg
     4081 ctccacgccc tgcaaggcct cgtacaagtg cgacgagtgc ctggaaccct tcgaatactt
     4141 caagtgcatc tagctctgcg aataacttgt gcagaggctc cctgatccat gcgccgcttc
     4201 cacagtctca ccgtgcagag cgttacaccc gaagccgagg atgcgatgcg catcagtctg
     4261 caggtgccgg aggaactgcg cgagcagttc cggttcctgc cgggccagca cctgccgctg
     4321 caagtcgtcc tcgacggcag gaaaatacgc cgtacctaca gcatctgttc cgttcccgga
     4381 gagtggccgc tggagatcgg tgtgcgcgtg cagcccggcg gcctgttctc cgggtttgtc
     4441 acgtccgggt tgtcggccgg cagtacgctc gaggtcatgc cgccgttcgg gcagtttcac
     4501 gtcacctgcg atcccgtcgc agtcaccgct gcacgaggca aattccgggt cgcttttgcc
     4561 gctggcagcg gcattacgcc gattctctct atcatgcgca cgacgctgca gaacgagccg
     4621 ggcagtcgct tcctgctctt ttacggcaat cggcgccagc gcacgacgat gtttatagaa
     4681 gagctgtatg ccttgaaaaa ctgctacccg gaccggttgc agctcaactt tgtcttcagc
     4741 cgcgaggagc aggaattccc gctcatgagc ggccgcctgg acggcccgaa ggtgcgcgag
     4801 ttgtggaaag ccttcgtccg gcagaatccc gacgagtcct tcgtgtgcgg gccggacacg
     4861 atgatagata ccgtcagcga agcgctcgtg gagctaggca tggagagttc cgctgttcac
     4921 gttgagcgtt acggggtgcc gcgccgaccg actgcacagc caccgccggc acccgccgtg
     4981 gctcgcggtg cggatcacct ggcgagcgtg acggtggtaa tggacgggca tgagaagacc
     5041 ttcgatatgt ccgcggacag tagcaacatc gtggatgccg ccgcggaaaa aggcgtggag
     5101 cttccatatt cctgcaaggc cggcgtctgc gcgacgtgca ggacctggct tcgcgagggc
     5161 aaggtccgga tgcacgcgaa ctacggcctc gagccgtggg aagtcaaaaa aggcttcgtc
     5221 ctcgcatgcc agtcagttcc gatgaccgac accgtcgtgc tggactacga caaggtatga
     5281 gggtctgcgg cagccgaccc accaggctct cccgggcgcc gtaggagccc gccctgcggg
     5341 cgcgtgcgtg catgcagcga actggccggc atggccggtc cctgcgagcg attcgaacgc
     5401 tgataagtct gttcggcgcc gtaaggtttc aatggcatca ttgggctcag ttgttctgta
     5461 agatggctcg ccaccaaacg agggaaccgg tggcgctacg cagccggctc atttcacagt
     5521 ccggaactgc gttttcagga actgacgcgg cgggtctcgt ggccgaagcg tcatttatcg
     5581 atgcgccgga gcgcacatga gcgaaacagg taagaaatct gcagtaaccg gtttggccga
     5641 cctgccgccg gaccggaaga attcctacag ccgcgaggaa ctcctgcagt gtgctcgggg
     5701 tgagctgttc gacccggata gcgccaagct gcccttgccg gatatgctga tgaccgaccg
     5761 ggtaccgctg atcgcgtcaa gcggcgggca atacggcaag ggcgagatta tcgcggaact
     5821 cgatatcgac cccgacatgt ggtttttcaa atgccacttc gccggcgacc cggtcatgcc
     5881 cggctgcctc gggttggatg cgctgtggca gctggtgggc tttttcctgg tgtggcatgg
     5941 cttcaagggg cgcggcaggg cactcggcgt cggcaatgtc aagttcagcg gccagatttt
     6001 gccgacctca cgcctggtca catacaattt gcagatgaaa cggctgatca cgcgcaagct
     6061 gatcctcgcg atcgccgatg gaaccgtttc ggtggacgga cggcagatct acacggccga
     6121 ggatctgcgg gtcgggctat tcacctccac cgacaacttt tagcgcgccc tgttgattat
     6181 tcgtgacggg tagggaagtg cagggctcct ttccagtttt atttcgtccg gcagttcatt
     6241 taggagaata gatgcggcgt gtcgcaataa ccggtctagg tgtcgtttcc tgcctcggta
     6301 acaaccagga ggaagtgctg gagtcgctga aggcaggccg ttccggtatc acgttcaacg
     6361 agaagtttca cgagatgggc cttcgcagcc aggtctgcgg cagcgtggcg ctggacatct
     6421 ccgggcacat cgaccgcaag gtccgccgct tcatgggcga tgccgcggcg tattcctaca
     6481 tcgcaatgca gcaggcaata gaagatgcca gcctcgaaga cacggacgtc tcgaacccgc
     6541 gcaccggcct cattgccgcc tccggcggcg cgtcgagctc caacattgtc ctgagcgccg
     6601 acacgctgcg gcaaaaggga gttcgcaaga tcggccctta catggttccc cgcaccatgg
     6661 gcagcaccgt ttctgcatgc ctggcgacgc cgttcaagat caagggcgtc aactattcga
     6721 ttacctcggc ctgcgcgacc agtgcgcatt gcatcggcgc ggcgatggaa cagatacaat
     6781 tcggcaagca ggacatcgtt tttgccggcg gcggggaaga ggaagactgg accctggcga
     6841 gcctcttcga cgcgatgggc gccctgtcct cgaactacaa cgacgcaccc ggcactgcat
     6901 cgcgtccgta tgaccagaat cgtgacggct tcgtcatcgc cgccggcgcg ggaatggttg
     6961 tcgtcgagga actcgagcac gcccgcgcac gcggcgcaaa aatctacgcc gagatcgtgg
     7021 gttacggagc aaactcggat ggattcgaca tggtggcgcc ttccggcgaa ggtgcggtcc
     7081 gctgcatgca gctcgcgcgc tctaccgtga acgcgcccgt cgactacatc aacacgcacg
     7141 gcaccagcac gccggtcgga gatacccgcg agctgcaggc gcttcgggag gttttcggca
     7201 acgagatccc gcgattcgcc tccagcaagt ctctcggcgg gcacagcctc ggcgcgaccg
     7261 gggtgcagga gtcaatacat tgcctgctca tgctgcaaga cgatttcatc gcgccgtcca
     7321 tcaacgtctt cgatatcgat cctgcggccg aaggtatgcc tctcgttacg cagcgcgtcg
     7381 acagcgcagg tttgcgaact gtgatgacga acagcttcgg cttcggcggc actaattcct
     7441 cgctgatctt tcagcggctg gacgaaaaat agggtacgtg tccatggaga aactgagcga
     7501 gcaccgcgca ttcgagggaa cggtagggtt ttatcgccat cgctccgatg tcaacaactg
     7561 cgacatgcaa ttttccgtgt tcgtgccgcc aggcgcggcg aacgctcccg ccccgtccct
     7621 tacctggctt tcagggctga cctgcacgga agaaacgttc atgatcaagg ccggcgcgca
     7681 aaggatcgcc gccgaactgg gcctgatgct gatcgcaccg gacaccagcc cgcgcggcaa
     7741 aaacgtaccg gacgaccccg acggcgcata cgacttcggc ctgggcgcgg gcttctatgt
     7801 caacgccacg cagccgccat ggaaccggca ctatcatatg tacgactacg tgacgaagga
     7861 gttgcctgac ctggtgttcg ccgagtttcc gggcgaccgc tcgcggcaag ggatcttcgg
     7921 ccattcgatg ggagggcacg gtgcactcac catcggcctc aagaatcccg caacgtacac
     7981 gagcatctcg gcgtttgcgc cgattgcaag cccggtcagg tgcccgtggg gcgagaaggc
     8041 tctcggcaat tacctcggct cctctgtgga gcacaggaag agctggctcg agtacgatgc
     8101 gaccgagctg gtcaagtcca tcggccaggc tggcgggata tccaaacccg ttaacgagat
     8161 tctcgtcgac cagggattgg ccgaccagtt cctgcaagag caattgcacc cacacctcct
     8221 ggaagccgcc tgccgcgagc agggcgttgc gctgaagatg gcccgccatg aaggttacga
     8281 ccatgggtat tacttcat
//
LOCUS       DASUOM010000464         7431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1388380,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000464 DASUOM010000000
VERSION     DASUOM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388380"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..342)
                     /locus_tag="VFY27_09615"
     CDS             complement(76..342)
                     /locus_tag="VFY27_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765813.1"
                     /translation="MLTVTDTVLEQFDKAIANASDADNEDRCLRIVRSENAGGLSLAL
                     QSPAPNDTTYEYEGRTVLAVPETYAEFCEDKTLDIDEEGRLTLA"
     gene            complement(405..1838)
                     /locus_tag="VFY27_09620"
     CDS             complement(405..1838)
                     /locus_tag="VFY27_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HEX5765814.1"
                     /translation="MIEASFLVILLAFVAIGLASAMRARKSRTDYYLASRAISPWLCG
                     LSAVATNNSGYMFIGVIGFTYATGLSAAWLMLGWILGDFVGSLFIHKKLRTSTERTDE
                     ASFAAVVARWHGEEFRVWRRIAAVVMVAFLGAYAAAQISAGGKALQGVLGWEPAAGAL
                     VVAAMILAYSVAGGIRASIWTDAAQSIVMLAAMMLLFAVALRGQGGFPETIEKLSEIP
                     GYLDLFPATLLFPGAPGMGLFVLGWMFAGFSVVGQAHVMVRFMALDESGNMMRARAWY
                     YSFFTAFYALATGVGLLSRLYLPELGGMDPELALPTMALELLPPVLVGVILAGIFAAT
                     MSTADSLVLSCSAAITHDLLPGRFASTWQAKIATAGVTLLALGIAMSETRSVFQLVIL
                     SWSTLASAFAPLLTIYAAGRRVSEAGAIVAMAGGVAGALLWRAAELHTMVYEGLPGIM
                     IGLAIAFALSHASEPRPLPVSSYANEA"
     gene            complement(1893..3395)
                     /locus_tag="VFY27_09625"
     CDS             complement(1893..3395)
                     /locus_tag="VFY27_09625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5765815.1"
                     /translation="MTREVERQDVQQRATHTVEKIGGTSMSDARSVLGNIFLGRRSGA
                     ELYNRIFIVSAYSGITDKLLEDKKTGEPGVHALFAGSESEWAWGDAISEVSSDMRAIN
                     ARIFADHPDRQVADRFVRERIEGVRSCLIDLHRLCSYGHFSLSEHLDTVREMLAAVGE
                     AHSAHNSTLLLKQHDVNAVFVDLTGWREEANWSFDNRIARALSDIDLSNTLPVLTGYA
                     HSRAGTIQSYGRGYSEVTLSRVAVLTRAREAVIHKEFHLSSGDPKLIGRERVRKIERT
                     NYDVADQLSNMGMEAIHPRAAKGLRQANIPLRIRNTFDPNDNGSQITGDYVSGIPQAE
                     IITGMRSVIALEFFEQDMVGVKGYDAAILETLKRHSVRVVTKTSNANTIVHYLAGSLK
                     AVHRVVNDLRESFGSARVTTRKVAIVSAIGSDLDVTGLTALAVGALAKAGVEVLGLHQ
                     LIRNVDILFVLAEEDYDEAMIALHGALIERAGQQSKQASGSSASRPQRAA"
     gene            complement(3392..4339)
                     /gene="thpD"
                     /locus_tag="VFY27_09630"
     CDS             complement(3392..4339)
                     /gene="thpD"
                     /locus_tag="VFY27_09630"
                     /EC_number="1.14.11.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydroxylase"
                     /protein_id="HEX5765816.1"
                     /translation="MNGEVAAKPREDAYPSRQSTEAQVLPRLDDVVHGSWTPDAPLYE
                     EDARAFQRDGFLVMRDIFDAGEVETLCSAATRLRGTGRSALEPETLISEPGSGHVRSV
                     FRIHEQDPTFARLAVDARLAEVARFLLNDEIYIHQSRMNYKPGFRGKEFYWHSDFETW
                     HVEDGMPRMRALSMSVLLTENTPHNGPLMLIPGSHRHYVTCVGETPENHYRSSLKQQE
                     YGVPDDESLDWLAAGGGIAAPIGSAGTVIIFDCNTMHGSASNITPLPRSNAFIVYNAL
                     CNRLEAPFGPATPRPEFIATRMSLAPVQAVTGPLVRSSA"
     gene            complement(4336..4728)
                     /locus_tag="VFY27_09635"
     CDS             complement(4336..4728)
                     /locus_tag="VFY27_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX5765817.1"
                     /translation="MIVRNLNDAKKSRRLVETAGWNSVRLLLKEDAMGFSFHITTIYE
                     GAELPMHYRNHLESVYCVGGEGSVENQETGETFPIRPGVMYALDKHDPHILRATSEMT
                     MACVFNPPLNGLEVHDESGAYPLQAEAV"
     gene            complement(4742..6046)
                     /gene="ectB"
                     /locus_tag="VFY27_09640"
     CDS             complement(4742..6046)
                     /gene="ectB"
                     /locus_tag="VFY27_09640"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX5765818.1"
                     /translation="MSEQNKDSDPAVETFELLESQVRSYARNFPRIFHRASGSWLYTR
                     DGAKYLDFLMGCSTLNYGHNHPDLKRALIEYISSDGITHSLDMYSESKEKFLGEFHET
                     ILEPRKLDYVVQFPGPTGANAVEAALKLARKVTGRQNVIAFTNGFHGVTLGALATTAN
                     GFHRNGAGVALSGVTRMPFDGYFGPSVDTATYLDRMLEDGSSGVDAPAAVIVETVQGE
                     GGLNVADYDWLRNIAAIARKHGALFIVDDIQAGAGRTGTFFSFEPAKVKPDIVTLAKS
                     ISGYGLPLALVLIDRTFDEWLPGEHNGTFRGNCHAFVTARAALEHFWRDSAFEQSIYD
                     KGQLLKTKLEDVARANGFARTAVKGRGMMRGLEMPSGELAGLVVKKAFADGLVIETSG
                     SDGEIVKCLAALTISDEDLIRGLDIMARSVRVALDRADRAAA"
     gene            complement(6069..6635)
                     /gene="ectA"
                     /locus_tag="VFY27_09645"
     CDS             complement(6069..6635)
                     /gene="ectA"
                     /locus_tag="VFY27_09645"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HEX5765819.1"
                     /translation="MHVPNEDNGAGGIRDEAASKQVLLRKPSSTDGSRIWRLVAACPP
                     LDQNSMYCNLLQCRDFAETSVLAEVHGEAVGWISGYRRPDDPSTLFVWQVAVHERARG
                     LGLARKMLFGLLDRQELRDVARMQTTITPDNHASHALFRSFAERANAVLEATDCFDEK
                     RHFEGRHASERLITIGPLNSNRNARSAA"
     gene            6858..7343
                     /locus_tag="VFY27_09650"
     CDS             6858..7343
                     /locus_tag="VFY27_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5765820.1"
                     /translation="MENRSDTALIALRRILRATELNSRLLASQTGLTTSQFIILQIVA
                     RENRVLPSAVAKSAGLTQATVTSLVDKLARHGLVNRRRDTEDRRRIWIEVTAAGRKAL
                     AASPDLLQDRFQTAFLRLADWEQSMIIAALERVSTMLDAATLDASPILDIGELDRLLN
                     P"
BASE COUNT         1399 a         2350 c         2218 g         1464 t
ORIGIN      
        1 gatgctgagc tggactacaa ggacggcgtc ctggacgaaa tcgtcatttc cggccgagac
       61 tagacgcaca cagcgtcagg ccagcgtgag ccttccttcc tcatcgatgt ccagcgtctt
      121 gtcctcgcag aattccgcgt aggtctcggg aacggcgagg accgtgcgac cttcatactc
      181 gtaggtcgtg tcattgggcg ccggggactg cagggccagc gagaggccgc cggcgttttc
      241 actccgaaca atgcgcaagc accggtcctc gttgtcggcg tcagacgcgt tggcaattgc
      301 cttgtcgaac tgttccagaa ctgtatcggt tacggttagc atgtttcctc cttctgcctt
      361 ctgaaaactg ccgcctgccg aaacaggcag caccgaaaaa tctctcaagc ctcgttcgca
      421 taactgctga cgggcagtgg gcgcggttcc gatgcatgcg aaagtgcgaa cgcgatagcc
      481 aggccgatca tgatccccgg caatccctcg tagaccatgg tgtgcagttc cgctgcccgc
      541 cacagcaacg ccccggcaac gccgcccgcc atggccacaa tggcaccggc ttcgctcacg
      601 cgtctccctg ccgcgtaaat cgtcagcaag ggcgcgaacg ctgatgccag tgtggaccag
      661 gacaagatga ccagctggaa gacactgcgg gtctcgctca tcgcgatccc gagagcgagc
      721 agggtgaccc ccgccgtcgc aatcttcgcc tgccaggtgc tggcgaaccg cccgggcaac
      781 aaatcgtgtg taatcgcggc cgagcagcta agtaccagtg agtctgccgt tgacatcgtc
      841 gccgcgaaga ttcctgcaag aatcacgccg accagcaccg gcggaagaag ctcgagcgcc
      901 atcgtcggca gcgcaagttc cggatccatg ccgcccaatt ccggcaggta aagccgcgac
      961 agcaggccga cgcccgtcgc gagcgcataa aacgccgtga aaaagctgta gtaccaggcg
     1021 cgagcgcgca tcatgttccc gctttcgtcc agcgccatga acctgaccat gacatgcgcc
     1081 tgcccgacga cggaaaatcc tgcaaacatc cacccgagca cgaacagccc catacccggt
     1141 gcgccgggaa acagcagggt ggcgggaaac aggtccaggt aaccggggat ctcagagagt
     1201 ttctctatgg tttccggaaa cccaccctgg ccccgcagag cgaccgcgaa cagcagcatc
     1261 atcgccgcca gcatcacgat ggattgtgct gcatccgtcc agatggatgc ccggatgccg
     1321 ccggcgacgc tgtaggcgag gatcatcgcg gcaacaacga gcgcgcctgc cgcgggttcc
     1381 catcccagca cgccctgcag cgccttgccg ccggcactga tttgcgcagc cgcgtaggcc
     1441 cccaggaagg ccaccatgac cacggcggcg atcctccgcc acacgcggaa ctcctcgccg
     1501 tgccagcgcg cgacgaccgc ggcgaaggac gcttcgtcgg tcctttccgt ggatgtcctc
     1561 aatttcttgt ggatgaagag cgagccgaca aagtcgccga ggatccagcc aagcatcagc
     1621 caggccgcgg aaaggccggt agcgtacgta aaaccgataa cgccgatgaa catgtagccg
     1681 gaattattgg tcgccaccgc cgacaggccg cacagccacg gcgaaatggc gcgactggcg
     1741 aggtagtagt cggttcttga tttccgggcc cgcatcgccg acgcgagccc gattgccacg
     1801 aaggccagca gaatcaccag gaacgatgcc tcgatcatct ttcgatcatc tttcgatctt
     1861 ccggcggtaa tcctatgggc gcaggcttct cgtcacgcag cccgctgcgg cctgcttgca
     1921 gacgatccgg aagcctgctt cgactgctgc cctgcccgtt cgatcagggc gccgtgcagc
     1981 gcgatcatcg cctcatcgta atcttcttcc gcgaggacga agagtatgtc gacgttgcga
     2041 atcaactggt gcaggccgag cacctcgaca cctgcctttg ccagcgcccc caccgccagg
     2101 gccgtaagac ctgtcacgtc gaggtcgctg ccgattgccg agacgatagc tactttgcgc
     2161 gtcgtcaccc gagcggagcc gaagctttca cgcaggtcgt tcaccacccg gtgcacggcc
     2221 ttcaatgagc cggcgagata gtggacgatc gtgttggcat tcgacgtttt cgttacaacc
     2281 ctcacgctgt gccgcttgag cgtctccagg atcgccgcgt cataaccctt gactcccacc
     2341 atatcctgct cgaagaattc caacgcgatg acgctgcgca tgccggtgat aatctcagcc
     2401 tggggaatac cggagacgta atcgccggtt atctgggagc cgttgtcgtt cgggtcgaag
     2461 gtattgcgga tgcgaagggg tatgtttgcc tgccgcagtc ccttggcggc gcgagggtga
     2521 attgcctcca ttcccatatt ggaaagctgg tctgcgacgt catagttggt ccgttcgatc
     2581 ttgcgcacgc gttcccgccc gatcagtttc ggatcgccgc tcgacaggtg aaattccttg
     2641 tggatcacag cttcccgcgc gcgggtgagt accgccaccc gcgaaagcgt gacctcgctg
     2701 tagccgcggc cgtagctctg gatcgtgccg gcgcgactat gggcatagcc cgtgaggaca
     2761 ggcagcgtgt tcgacaggtc aatatcgctc agcgccctgg cgatgcggtt gtcgaaactc
     2821 cagttcgcct cttcccgcca gccggtaagg tcgacaaaca ccgcgttcac gtcatgctgt
     2881 ttcagcagta gcgtcgagtt atgcgcgcta tgtgcttctc cgaccgccgc cagcatttcc
     2941 cgaaccgtat cgaggtgctc actcaggctg aaatgaccgt acgagcacag gcgatgcagg
     3001 tcgatcaggc aactcctgac gccctcgatg cgctcccgca cgaaacggtc cgcaacttga
     3061 cggtcgggat ggtcggcgaa gatccgggca ttgatggcgc gcatgtcgct gctcacctcg
     3121 ctgatcgcat cgccccaagc ccattccgat tccgatcccg caaacagcgc atgtaccccg
     3181 ggctcgcccg tcttcttgtc ctccagcagc ttgtcggtga tgccgctgta ggccgacacg
     3241 atgaagatcc ggttataaag ttcggcacca ctgcgccgac cgaggaatat gttgccgagc
     3301 acggaccggg catccgacat cgaggtgccg ccgatcttct cgaccgtgtg cgtcgcgcgc
     3361 tgctgcacgt cttgccgttc gacttcccgg gtcacgctga gctccgtacc agcgggcccg
     3421 ttaccgcctg caccggcgcc aggctcattc gcgttgcgat gaactcggga cgaggggtcg
     3481 cggggccgaa gggcgcttcg aggcggttgc acaacgcgtt gtagacgata aatgcattac
     3541 tccgcggaag cggcgtgatg ttgctggccg aaccgtgcat ggtattgcag tcgaaaataa
     3601 tgaccgtacc cgcggagccg ataggcgcgg cgatgcctcc cccggcggcc agccagtcca
     3661 gcgactcgtc gtccggcacg ccgtactcct gctgtttgag ggaactcctg tagtggtttt
     3721 ccggcgtctc gccgacgcag gtcacgtagt gccggtgtga acccggtatg agcatgaggg
     3781 gcccgttgtg cggcgtattc tcggtcagca gcaccgacat cgacaaagcg cgcatccgcg
     3841 gcatcccgtc ctcgacatgc catgtttcaa agtcggagtg ccagtagaat tcctttccgc
     3901 ggaaaccagg cttgtagttc atgcgtgact ggtgaatata aatctcgtcg ttcagcagga
     3961 accgcgcaac ctccgcgagc cgggcgtcaa cggcaagcct cgcgaacgtc gggtcctgtt
     4021 cgtgtatgcg aaagaccgaa cgcacatggc cggagccggg ttcgctgatc agcgtttccg
     4081 gctccagcgc gctgcgtcct gtgccgcgca acctggttgc cgcgctgcaa agggtctcca
     4141 cttcaccggc gtcgaaaata tcccgcatta cgaggaaacc gtctcgctgg aatgccctgg
     4201 catcctcctc gtagagcggc gcgtcagggg tccagcttcc atgtacgacg tcgtcgagcc
     4261 gcggaagcac ctgtgcctcc gtcgactgcc gggaggggta tgcgtcttcc cggggtttcg
     4321 cggcgacctc cccgttcaca ccgcttccgc ctgcagtggg taagcgccgc tttcgtcgtg
     4381 gacctcgagc ccgttgaggg gcgggttgaa gacgcaggcc atcgtcatct ccgaggtggc
     4441 ccgaagtatg tgtgggtcgt gcttgtccaa cgcatacatg acgccgggac gtatcggaaa
     4501 cgtctcgccc gtttcctggt tctcgacaga gccctcgcct ccgacgcagt aaacggactc
     4561 caggtggttc cggtagtgca tcggcagctc ggcgccctcg taaatggttg taatgtggaa
     4621 agagaagccc atcgcgtcct ccttcaacaa caggcgcacg ctgttccagc ccgctgtctc
     4681 gaccagtcgc ctgctcttct tcgcatcatt aagatttcgc acgatcatgt gtatacctcg
     4741 tttacgccgc tgcgcggtcc gccctgtcca gcgcgaccct taccgaacgc gccatgatgt
     4801 cgagaccgcg tatcaggtct tcgtcggaga tcgtcaacgc cgccaggcac ttgacgattt
     4861 cgccatcact accgctcgtt tcgataacca gtccgtcagc gaatgctttc ttgaccacca
     4921 ggcctgcgag ttcgccggaa ggcatctcga gcccgcgcat cattccacga ccctttacgg
     4981 cggtccgggc aaagccattc gcgcgagcca catcttcgag cttcgttttc agcagctggc
     5041 ccttgtcgta tatcgactgc tcgaaggcgc tgtcgcgcca gaagtgctcg agcgccgcgc
     5101 gagctgtgac gaatgcgtgg caattgccgc ggaaggtgcc gttatgctcg cccggcagcc
     5161 attcgtcgaa cgtccggtcg atcaacacga gcgctagcgg caggccatac ccggaaatgg
     5221 acttggccag cgtgacgatg tcgggcttta ccttcgccgg ttcgaagctg aagaacgtgc
     5281 cggtccgacc tgcgcccgcc tggatgtcgt cgacgataaa cagcgcaccg tgtttgcgcg
     5341 ctattgccgc gatgttcctg agccagtcgt aatcggcaac gttcaagcca ccctcgccct
     5401 gtaccgtttc gacgattact gcggcgggag cgtcgacgcc gctggatccg tcctcgagca
     5461 tcctgtccag gtatgtggca gtgtcgaccg atggtccgaa gtagccgtcg aagggcattc
     5521 gcgtgacgcc gctcaaagct acaccggcgc cgttacgatg gaatccatta gcggtggtcg
     5581 caagggcgcc gagggtgacg ccgtgaaaac cattggtgaa cgcaatgacg ttttggcgtc
     5641 cggtaacttt gcgcgcgagt ttcagtgccg cttcgacggc gtttgcgccg gtgggaccgg
     5701 ggaactgcac gacgtagtcg agcttgcgag gctcgaggat cgtctcgtgg aactcgccaa
     5761 ggaatttttc cttactctcg ctgtacatat ctaggctatg cgtgatgccg tcgctgctga
     5821 tgtactcgat cagtgctctc ttgagatcgg gatggttgtg cccgtagttg agtgtcgagc
     5881 aacccatcag gaagtccagg tatttggcac cgtctcgggt atacagccac gaaccgctgg
     5941 cgcggtgaaa aattcgcgga aagtttcgcg cgtagcttcg cacctgcgat tcgagcagtt
     6001 cgaaagtctc gacagcaggg tcgctgtcct tgttctgttc agacatggtg ttcctctggt
     6061 gtgagtggtt acgcggcgct gcgcgcgttt ctgtttgaat tcagcgggcc aatcgttatg
     6121 agccgttcgc tcgcatgcct gccctcgaag tgccgcttct cgtcgaagca gtcggtagcc
     6181 tcaaggacag cattcgcccg ctcggcaaat gagcggaaca atgcatgcga cgcgtgattg
     6241 tcaggggtta tcgtggtctg cattcgagcg acgtcccgca gttcctggcg gtcgagcaga
     6301 ccgaacagca tcttgcgcgc cagtcccagc ccgcgagcgc gttcgtgaac cgctacctgc
     6361 catacaaaaa gggtcgacgg atcgtcaggg cggcggtagc cggaaatcca gccgacagcc
     6421 tcgccatgca cttccgcaag cactgacgtt tcggcgaaat cgcggcactg caggaggttg
     6481 cagtacatcg agttctggtc cagcggcggg caggcggcaa cgagtcgcca gatgcgcgag
     6541 ccgtccgtgg aggaaggctt cctgagcaat acctgcttcg atgccgcttc gtcgcggatc
     6601 ccgcccgctc cgttatcttc attgggcacg tgcataatta gttcgatagt ctaactaagt
     6661 gtacggcgta tttaaaccgc tcgatggccg ctcttccgta aaacgccaca tagttgctta
     6721 gtacagcgaa gtaactcggg agctgcccgc ggacactgct ggcgagcagc ggcggaatct
     6781 gcgaaaaaga aatcctcagg agtttgcgcg gtagacagta ttcctctcct tagcctgcag
     6841 catcgaagga ttcgccgatg gaaaaccgtt ccgacaccgc gctgatcgcg ctgcgccgca
     6901 tcctgcgagc gacagagttg aactcgcggc tcctcgccag ccagacgggg ctgacaacgt
     6961 cgcagttcat catcctgcag atcgtcgctc gcgagaacag ggtgctaccg agcgccgtcg
     7021 cgaagtcggc ggggctgacg caggcgacgg tgacgtcgct ggtagacaag cttgcgcgtc
     7081 acgggctcgt gaaccgccgc cgcgacacgg aggaccggcg gcgcatctgg atagaggtaa
     7141 ctgcagccgg gcgcaaggcg cttgccgctt cgccggacct tctccaggac cgtttccaga
     7201 ccgcatttct caggctggca gactgggagc agtcgatgat catcgcggcg ctggagcgcg
     7261 tatccacaat gctggacgcg gctacactcg atgcttcgcc catcctcgac atcggtgagc
     7321 tggatcgttt gctgaaccca tgattccttc cgccgcgggc ggcttccgga aggctttgag
     7381 aaccctgcca ctcttcagtg gccaggcctg ccatcctccg ccgcccgcgg c
//
LOCUS       DASUOM010000465         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_898133,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000465 DASUOM010000000
VERSION     DASUOM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898133"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /gene="bamD"
                     /locus_tag="VFY27_09655"
     CDS             <1..2052
                     /gene="bamD"
                     /locus_tag="VFY27_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5765821.1"
                     /translation="AASVYQSFIALNPLHQKSPHFSMRVIEIYEEGGFPKLVVDSKKE
                     FATNYALDAEYWNHFNAEDSAAVLEFLKTNLTDLANHYHALYQNEELVDERPASFAEA
                     SRWYREFLQSFPEDPASPPINYQLADLLLENEDFGEAALEYERTAYGYSPHDQASAAG
                     YAAIYAHRENLKLATAARQLALKQATVASSLRFADTFPDHEQAPVVLGAAADDLYDMQ
                     EFATAIESARKLIERYPGADPELTRSAWVVVAHSSIDTAEYQDAEHAYMQVLGLTADD
                     DESRTAIIDGLAASIYKQGEQANLLEDYRSAADHFLRIRELAPTSSIRTAAEYDAASA
                     LVKLEDWSGAAAVLEDFRSAFPGHELNPDATKQLAFIYRKDGQIDRSAAEHERIAAES
                     EDPELRREALLLAGELYEEAGSVDSALRVYLGYAEQFPRPLDIALETRSKVAEIFRTK
                     NDLPRYYQELDEIVALDKTAGADRTDRSKYLAARAALVLTEQLFERFVKLKLHQPFEE
                     RLAEKQQLMDAAMAAFEDLVQYEVAEVTAAATFYIGETFYEFSDALLESERPANLTAA
                     ELADYELAIEEAAYPFEEQAIDVHEKNFELLAVGVFNPWVQKSLDKLTVLMPGRYAKN
                     EISTGFMGSIDTYAYRMPVAPSLGVDETDGLDVSHEAVPAPTGTAVQAALIDSSVVPS
                     Q"
     gene            2068..2718
                     /locus_tag="VFY27_09660"
     CDS             2068..2718
                     /locus_tag="VFY27_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5765822.1"
                     /translation="MCAPAEHSAPAASRAGLVMLAAALLTAGCGAPGASKKPARIEIQ
                     EEVGFVITEEARINSEVRADYDEALRLLGQGSTAEGIALLVKAAEEAPQLSAPRIDLG
                     IAYQQAGDLVAAEEQLQKALAINPEHPVVHNELGIVYRKSGRFAEARTSYEAALAIYP
                     SFHFARRNLAILCDLYLADMDCALEQYLAYVETAPEDEEAAMWLADIRNRLNQQVQ"
     gene            2721..3050
                     /locus_tag="VFY27_09665"
     CDS             2721..3050
                     /locus_tag="VFY27_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765823.1"
                     /translation="MRSIACGAVMLGFAFCAMAEEPEQEAADDKTEIEEESSVKSGDG
                     QPTTMSGMSILGNEEAPKSLVIVPWKSSELGAELGLTNTLDDRAQPIDRDVFLRELSY
                     YELRSAK"
     gene            3194..3850
                     /locus_tag="VFY27_09670"
     CDS             3194..3850
                     /locus_tag="VFY27_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5765824.1"
                     /translation="MDFFYSMVGFFTSGGLFMFPILLVFAVGAAIAVERYVTLTLVTN
                     KNQNVWDKVQPLLSKGDFEAARKVTGDDDSTISQVLNMGLSLQGAVRRREDIEIAMEE
                     SMMEIVPRLEKRTPYVALASSIATLLGLLGTIMGLIQAFTAVANANPAEKADLLSASI
                     SVAMNTTAFGLMVAIPLLVTHVVLTSKTGDIVDSLEMASVKALNVFAKRAKRQLDMAA
                     "
     gene            3863..>4145
                     /locus_tag="VFY27_09675"
     CDS             3863..>4145
                     /locus_tag="VFY27_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5765825.1"
                     /translation="MARRHHYKRRSKDATEIDVTTFLNLMVVLVPFLLITAVFSRLSI
                     VELNLPSSAGGPSSSDVPFTVEVIVREAGIEITNGTSVIAAIPNVDGEYD"
BASE COUNT          886 a         1203 c         1296 g          760 t
ORIGIN      
        1 gcggcgtcgg tttaccagtc gttcatcgcg ctgaaccctc tccaccagaa gtcgccgcac
       61 ttcagcatgc gcgtgatcga gatctatgaa gaaggcggtt ttccgaagct cgtagtcgac
      121 tcgaagaagg aatttgccac caactatgca ctggatgccg agtactggaa ccacttcaac
      181 gccgaggatt cagccgctgt tcttgaattc ctgaagacga acctgaccga cctggcgaac
      241 cactatcatg cgctgtacca gaacgaagaa ctggtcgacg agcggccggc cagtttcgcc
      301 gaggcttccc gatggtaccg ggagtttctc cagtcgttcc cggaggaccc ggcatcgccc
      361 ccgatcaact accagctcgc cgacctgctc ctcgagaacg aggacttcgg cgaagcagcc
      421 ctggagtacg agcgaacggc atacggctat tcgccgcacg accaggcatc cgccgccggc
      481 tatgccgcga tctacgcaca ccgcgagaat ctgaagcttg ccacggccgc gcggcagctg
      541 gcgctgaagc aggcgacggt agccagctca ttgagattcg cggacacctt cccggatcac
      601 gagcaggcac cggtggtgct gggcgccgca gccgacgatc tctacgacat gcaggaattc
      661 gcgacagcca tcgaatcggc gcgcaagctg atcgaacgct acccgggtgc ggacccggaa
      721 ctgacgcggt ccgcgtgggt ggtggtggcg cactcctcga tcgatacggc ggagtatcag
      781 gatgccgagc acgcgtacat gcaggtgctc ggcctgacgg cggacgacga cgagtcgcgc
      841 acggcgataa tcgacggttt ggcagcgtcg atttacaagc agggcgagca ggccaacctg
      901 ctggaggact accgcagcgc tgcggaccac ttcctcagga tcagggaact ggctcccacc
      961 tcgagcatcc gtaccgcggc ggagtacgac gcggcgtcgg cgctggtcaa gctcgaggac
     1021 tggtccggtg ccgcggccgt actggaggac tttcgcagcg ccttccccgg gcatgagctg
     1081 aatccggatg caacaaagca gctcgcgttc atctatcgga aggacgggca gatcgatcgc
     1141 tcggccgcgg aacacgagcg catcgcggcg gaatccgagg atcctgaact gaggcgcgaa
     1201 gcgctgctcc tggccggcga gctgtacgag gaagcgggca gcgtcgacag cgcgttgaga
     1261 gtctacctgg gctacgcgga gcagtttccg cggccgctgg acatcgcgct ggaaacccgt
     1321 tccaaggtag cggagatctt caggacgaag aacgatctcc cgcgctatta ccaggagttg
     1381 gatgagatcg tcgccctcga caaaaccgcc ggagcagaca ggacggatcg cagcaagtat
     1441 ctcgccgcca gggccgcact cgtactgacc gagcagctct tcgagcgctt cgtgaagctg
     1501 aaactgcatc aaccattcga ggagcggcta gccgagaagc agcagctgat ggacgccgca
     1561 atggcggcgt tcgaagacct ggtgcagtac gaagtggcgg aggtgacggc cgcggcgacg
     1621 ttctacatcg gcgagacgtt ctacgaattc agcgacgcgt tgctcgaatc ggagcgtcca
     1681 gccaacctga cggctgccga gttggcggat tatgagctgg cgatcgaaga agcagcttac
     1741 cccttcgagg aacaggcgat cgatgttcac gagaagaatt tcgaattgct ggcagtcggg
     1801 gtattcaacc cctgggtgca gaagagcctc gacaagctca cggtgctcat gccgggccgc
     1861 tacgcgaaga atgagataag cacgggcttc atgggttcga tcgataccta cgcgtatcgg
     1921 atgccggtgg cgcccagcct cggcgttgac gaaacagacg ggctcgacgt ctcgcacgag
     1981 gctgtgccgg cgccgaccgg gactgccgtg caggccgctt tgatcgacag cagcgtggtt
     2041 ccgtcgcagt gatatccggg gtgagtcatg tgtgcaccag ctgaacacag cgcaccggcg
     2101 gcctccaggg ccggccttgt gatgcttgcg gcagcgctgc tgactgcagg ttgcggcgcc
     2161 cccggcgcat cgaagaaacc tgcgcgcatc gaaatccagg aagaggtcgg cttcgtgatc
     2221 accgaagaag cgcgaatcaa cagtgaagtt cgcgcggact acgacgaagc cttgcgtctg
     2281 ctggggcagg gaagcactgc ggaaggaata gcgttgctgg tgaaggcagc ggaagaggcg
     2341 ccacaactca gtgcgcctcg catcgatctt ggaatcgcct accagcaggc gggcgatctc
     2401 gtggcggccg aagagcagct gcagaaggcc ctggcgatca atccggagca tcctgtcgta
     2461 cacaacgagc tagggattgt ttaccgcaag tccgggcgtt tcgctgaagc ccgcacgagt
     2521 tatgaggcag cactggccat ctacccgagc tttcatttcg cgagacgcaa cctggcgatt
     2581 ctgtgcgatt tgtacctcgc cgatatggac tgcgcgctcg agcagtacct ggcctatgtc
     2641 gagacggcgc cggaagacga agaggccgca atgtggctcg cagacattcg taaccggctg
     2701 aaccagcagg tgcaatgatg atgagatcaa tagcgtgtgg cgcggtgatg ctcggttttg
     2761 cgttctgtgc gatggcggag gagccggagc aggaagcagc cgacgacaag actgagatcg
     2821 aggaagaaag ctccgtgaaa tccggcgacg gacagccgac gacgatgtcg ggcatgtcca
     2881 tcctgggtaa cgaagaagca cccaagtcgc tcgtcatcgt tccctggaaa agctccgagc
     2941 tcggcgcaga actcggcctt accaacacgc tcgatgatcg tgcgcagccc atagacaggg
     3001 atgtgttcct gagagaactc agctactacg aattgcgatc cgccaagtag cagcgaaacc
     3061 gaatttccat aatcccgttt tttcattggc gtatccatac gcgaacgtgc gcttggtcac
     3121 actcctctct gtgacgtcgt tctaagcttg tcgaaagtgc agtccgaaaa ttctgttaaa
     3181 tgaggatctg gtaatggact tcttttattc gatggttggt tttttcacgt cgggcggcct
     3241 gttcatgttt ccgatactgc tggtcttcgc cgtcggagct gcgatcgcag tagagcgcta
     3301 cgtgacactc acccttgtga cgaacaaaaa ccagaacgtg tgggacaagg tccagccctt
     3361 gctctcgaaa ggagacttcg aagcggcgcg caaagtaacc ggcgacgacg actccacaat
     3421 ctcccaggtt ctgaacatgg gtttgtcgct ccagggcgcg gtgcggcgcc gggaggatat
     3481 cgagatcgcg atggaagaaa gcatgatgga aatcgtgccg cggctggaaa aacgtacgcc
     3541 gtacgtcgcg cttgcctcca gcattgccac gctgctggga ctgctgggca cgatcatggg
     3601 gctaatccag gcgtttaccg ctgtggcaaa tgcaaaccct gccgagaagg ccgatttgct
     3661 gtccgcgagc atctccgtcg cgatgaatac caccgccttc gggctcatgg ttgccattcc
     3721 actgctggtg acccacgtcg tgttgaccag caagacgggc gatatcgtgg acagcctgga
     3781 gatggcgtcg gtaaaggcgt tgaacgtctt tgccaaaagg gccaagcgcc agctcgacat
     3841 ggcggcctga ggcgcgacca gcatggccag gcggcaccat tacaaacggc gttccaagga
     3901 cgccaccgag atcgacgtca ccaccttcct gaacctgatg gtggtcctgg tgccgttctt
     3961 gctgattacg gcggtctttt ccaggttgtc gatagtcgaa ctcaatcttc cctcgagcgc
     4021 cggtggcccg tcgtccagtg acgtaccttt tacggtcgag gtaatcgtgc gcgaagccgg
     4081 cattgaaatt acgaacggca catcggtgat cgcggcgata cccaacgtgg acggcgagta
     4141 tgacc
//
LOCUS       DASUOM010000466         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1733533,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000466 DASUOM010000000
VERSION     DASUOM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733533"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFY27_09680"
     CDS             <1..739
                     /locus_tag="VFY27_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5765826.1"
                     /translation="NKKGFSGDVQAYEELRESARAGSSHCFYETTVGAALPIIRTMRD
                     LIDTGDRIHSVQGILSGTLAYLFNVYDGSRPFSDIVLAARESGYTEPDPRDDLSGMDV
                     ARKLTILARELGATVGIGDFPVQNLVPEPLRDVSLEEFLARLPEHDEDMHALFHDAKA
                     QNRILRYIAKLTANGSASVGLEMVEASSPFGNINLTDNLVQFVTDRYSANPLVVQGPG
                     AGPEVTASGVFSDLLRLAAFLGDGITV"
     gene            736..1698
                     /locus_tag="VFY27_09685"
     CDS             736..1698
                     /locus_tag="VFY27_09685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5765827.1"
                     /translation="MREESWTSAFAPASIGNVAVGFDMLGLALEGVGDTVSVRRRSEE
                     GVAVVAALDPDGRPHSELSVDPKKNTASIAAAALWLAAAAPGGLELVVRKGIPLQSGM
                     GSSAASAAAAVVAANALLDSPFEIQKLLPYALEGEKFASGGLHADNVAPSLLGGLVFC
                     PAALLPNAVPLKAPPGLSSVLVHPDLKIDTAMARRSLSNTCTMEQWLTQQGYLAMFML
                     ACERDDHEMVARSLRDVIIEPQRAASVPPFHYVKDAALDAGALGCSLSGSGPSIFALC
                     ENRHAAAVAEAMTKACSGTGVDCRSWISSMNSPGAEVLARIDHG"
     gene            1714..>2955
                     /gene="thrC"
                     /locus_tag="VFY27_09690"
     CDS             1714..>2955
                     /gene="thrC"
                     /locus_tag="VFY27_09690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5765828.1"
                     /translation="MKYVSTRGGDPVSLDQAIAAGIAKDGGLFLPDALPEFAVKDFED
                     AVSIHEVAQVLLRPFFEESALAPHLEEIISETFSFPIPLTELSVATGDLHMLELFHGP
                     TAAFKDIGAGFLAACLGRLQHDQSMPLTILVATSGDTGGAVAAAFNERPGMRVVVLFP
                     EGRVSERQARQLTGWGGNVLSLAVQGSFDDCQTLVKEAMAKPDLNARYRFSSANSINI
                     GRLLPQCIYYAHASLTRYRESGQKPSFIVPTGNLGNGLALLLCREMGLPVGDVILATN
                     ENRVIGDYLEGGEWLPTESKATLASAMDVGNPSNMERLLALIGDAGSLHRLVRAVSVS
                     DPQIEEEIREGNSDFGFAICPHTATACHVYRHLSDEERRMKDWIVVATAHPAKFEQVV
                     EPLTGQILVLPDSLRAILDRPS"
BASE COUNT          556 a          895 c          946 g          558 t
ORIGIN      
        1 gaacaagaaa gggttcagcg gcgacgtcca agcatacgag gaactacggg agagcgcgcg
       61 cgccggaagc tctcactgtt tctacgaaac cactgtaggc gccgcgctgc cgatcatccg
      121 gacgatgcgc gacctcatcg atacgggcga ccgtattcac tcggtccagg gaatcctgtc
      181 gggtacgctg gcctacctat tcaatgtgta tgacggaagt cgtcctttct ccgacatcgt
      241 gctggcggcg cgtgaatcgg gttatacgga gcccgacccg cgtgatgacc tctccggcat
      301 ggacgtcgcg cgcaaactga caattctcgc gcgcgaactg ggtgcaacgg tcggcatcgg
      361 ggatttcccg gtgcagaacc tggtcccgga gccgctgcgt gacgtgagcc tggaagagtt
      421 cctggcgcgc cttccggagc acgacgagga catgcatgcg ctgttccacg atgcgaaggc
      481 gcagaaccgc atccttcgat atatcgccaa gttgacagcc aatggcagcg cttcggtggg
      541 cctggaaatg gtggaagcat caagtccgtt cggtaacatc aacctgacgg acaatctggt
      601 gcagttcgtc acggatcgct actccgcgaa cccgctggtc gtccagggac ccggagccgg
      661 tcccgaggta accgcgagcg gcgtattcag cgatttgttg cgcctggcag cgttcctcgg
      721 cgacggcata acggtatgag agaagaaagc tggaccagcg cattcgcgcc tgcgtcgatc
      781 ggtaacgtgg cagtcggctt cgacatgctc gggctcgcgc tggagggcgt gggtgacact
      841 gtctccgttc gcaggcggag cgaggagggt gtcgctgtcg tggcggcact ggatcccgac
      901 ggccggcccc actcggaact gtccgtcgac ccgaaaaaga acaccgcttc gatcgccgct
      961 gccgcgctgt ggctcgccgc cgcggccccg ggcggtttgg aactggtcgt acgaaaaggc
     1021 attccgctgc aatccgggat gggaagctcg gcggcctcgg cggccgcggc ggtcgttgcc
     1081 gcgaatgcgc tgctcgactc gccgttcgag atccagaagc tgctgccgta tgccctggag
     1141 ggagagaagt tcgccagcgg cgggctgcac gcggacaacg tggcgcccag tctcctgggc
     1201 ggcctcgtgt tctgtcctgc ggcgctgctg ccgaatgcgg tacctttgaa ggctccgccg
     1261 gggttgagca gcgtgctggt gcacccggac ctgaaaatag acaccgcgat ggcgaggcga
     1321 agtctctcca atacctgcac aatggagcaa tggctgacgc aacagggata cctcgctatg
     1381 ttcatgctgg cctgtgaacg cgacgaccac gaaatggtcg cccgatcgct gcgcgacgtg
     1441 atcatagagc cgcagcgcgc tgcctccgtg ccgccatttc attatgtgaa ggacgccgcg
     1501 ctggatgcgg gcgcgctcgg ttgttccctg tcgggcagtg gtccgagcat tttcgccttg
     1561 tgcgaaaatc gtcacgctgc ggccgtcgct gaagccatga cgaaagcctg ttcaggaacg
     1621 ggagtcgact gcagatcctg gatcagttcc atgaattcac caggcgcaga ggtgctcgcc
     1681 aggattgacc atgggtagtt cgccggacac cacatgaagt atgtgagcac acgaggcggc
     1741 gatccggtct cgctcgacca ggcgattgcc gcaggcatag cgaaagacgg cggcttgttc
     1801 ctgccggacg cgttgccgga gtttgcagtc aaggacttcg aagatgcggt ttcaatccat
     1861 gaggtcgcgc aggtcctgtt gcgccctttc ttcgaagaat ccgcgctcgc cccgcatctc
     1921 gaggaaatta tcagcgagac cttctcgttc ccgattccgc tcacggagct ttccgtcgcc
     1981 accggcgacc tgcacatgct cgagctgttt catggtccca ccgccgcctt caaggacatc
     2041 ggtgcgggct tcctggcggc ctgcctgggc cgtttgcagc atgaccagtc gatgcccctg
     2101 acgattctcg tagccacatc gggcgatact ggcggcgccg tcgccgctgc gttcaatgag
     2161 cggccgggaa tgcgggttgt ggtcctcttt ccggaaggca gggtatccga gcgacaggcg
     2221 cggcaactca ccggttgggg cggcaacgtg ctctcgctgg cggtacaggg aagcttcgat
     2281 gactgccaaa cgctggtcaa ggaagccatg gcgaagcctg acctcaacgc gcgttaccgc
     2341 ttcagctcgg ccaacagcat caacatcgga cggctgctgc cacagtgcat ttactatgcc
     2401 cacgcgagtc ttacgcgtta ccgcgagagc ggccagaagc cttcctttat cgtaccgacc
     2461 ggaaatctgg gtaacgggct tgcgctgctg ctgtgcaggg agatgggact gccagtaggc
     2521 gacgtcatac tggcgacgaa tgaaaaccgc gtgatcggcg actacctgga agggggcgag
     2581 tggctgccga cggagagcaa ggcgactctt gcctccgcga tggatgtcgg taaccccagc
     2641 aacatggaaa ggctccttgc gctgatcggc gatgcgggat ctcttcacag gcttgtcagg
     2701 gccgtttccg tatctgaccc acagatcgag gaagaaatac gtgaaggcaa ctccgacttc
     2761 ggattcgcca tctgcccgca cacggcgacc gcctgccatg tctatcgcca cctgagcgac
     2821 gaggaacggc ggatgaagga ctggatcgtg gtggcgacgg cgcatcccgc gaagttcgag
     2881 caggtagtcg agccgctgac gggacaaata ctggttttac ccgattcgct tcgcgccatc
     2941 ctcgaccgcc cctcc
//
LOCUS       DASUOM010000467         5456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1382528,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000467 DASUOM010000000
VERSION     DASUOM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382528"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VFY27_09695"
     CDS             <1..1766
                     /locus_tag="VFY27_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5765829.1"
                     /translation="DQPEYYPPTRTGMRGSHPGSFEDAHAVRDNPNIVADAVDTGERF
                     DLIVAGAGISGLAAAHFYRKAAGPRAKILLLDNHDDFGGHAKRNEFHVNGKVLIMNGG
                     TAGISSPTPYSPVADGLLKHLGIHPTELAEATYDHDFYSKNNLGSAVFFDKETFGDDK
                     LVQQSGGRQSTEETAAFLARAPLSEAVRKDILAIETGRNDYMPGMSADAKKDRLSRMS
                     YADYLLKVVKADPAVISYYLHDTDGLGGCGIDAVSALDCWGVGFPGFQGLNLPRTATA
                     RMGYTPGKELETGGSYRFAFPDGNASVARLLVRDLIPDAVPGTTVEDSIAAEVKYGKL
                     DSNDNSVHIRLSSTVAHAVNARDGGVKVTYLRDGKAYCVRGKHSVLACWNMMIPFICP
                     ELPEEQKRALHYLVKTPLVYTSVALTNWRAFANLGVSRVRCPGSYHTSFSLDSVMDIG
                     DYHSAKSPDEPVMVHMTRAPARPGLGEREQHKAGRAELLTTPFSTFERHIRSQMNRAL
                     GSGGFDAANDIAGIAVNRWPHGYAPEYNSLVDPDEGLPLLTARARAGAIAIANADSGG
                     GAYTDVAIDQAHRAVMELLTA"
     gene            1807..3687
                     /locus_tag="VFY27_09700"
     CDS             1807..3687
                     /locus_tag="VFY27_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5765830.1"
                     /translation="MSSGLNISRRDFLNGVAMATAAGSMLSPMDLLAAQVSADYYPPL
                     RTGMRGSHPGSFEVAHGVSWAGHKYPTPKQQTDDVYDMIVVGGGISGLSSALFYHQRA
                     GVDKNVLVLDNHDDFGGHAKRNEFNVNGQTLIGYGGSQSIDGPGNFSAASSRMLKDVG
                     IVTERFYDYFDQDYMRNRNLRRAIYFSSEVYGREVTAPNILRGDFGDDPKQLDAIVAQ
                     YPISDESKAALLRLWRDETDHRPDLSRSEKIDFLGSIAYTKYLREYVGMPRQVTDLLR
                     DTILGLWGVGWDSLSALEAYRSGMPALQHMGLGELAEQGHERDEPYIFHFPDGNAGLA
                     RSLVRSLLPNAVPGSTMEDLVMSRIDYSLLDRRKSPTRIRLNSTAVNLHHTPDQKHVD
                     VTYVTDGDAYRVRAKHVVFAGYNAMLPYICGEVPPAQKEAIAYAEKVPLVYMSIAVRN
                     WHALAEMGYSSVFIPRSKYIYSFGLDFPVSMGDYRFTQDPQQPTVLHGSWVPNVPEQG
                     LSAREQHVAGRRQLYELSFDDFEQDIVEKMSGSLAPGGFDAERDIAAITVNRWPHGYA
                     YEYNDLSDPEGFTPQNGPHVAGRAQIGRISIANSDASAYAYVNGAIDAADRAVNEQMQ
                     TA"
     gene            complement(3847..4479)
                     /locus_tag="VFY27_09705"
     CDS             complement(3847..4479)
                     /locus_tag="VFY27_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5765831.1"
                     /translation="MSKEKPTGVARTELTSKGSRRVNEILAAASGILVEHGFLALNKR
                     SVASSLGISDGNVSYYFPTKESLWLAVIDYELAEYYRRHHPESHIADDDPQAAFDDYV
                     SRWIDEYQDPTVRIFFSQIITVAESNDRIATKRDEIYEAFVTTMLGLARRLKLKVHPR
                     KLERRVLTAIAVLEGLHAVSAFRPTLFADDSRYKLHIVSLVNAIVRGVAP"
     gene            complement(4527..>5456)
                     /locus_tag="VFY27_09710"
     CDS             complement(4527..>5456)
                     /locus_tag="VFY27_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5765832.1"
                     /translation="LDYTGWFQHEKDLRSLQATVSSLNTDGTDLVAVTESGERIRSRF
                     VVMATGFGSFTNVPLELASLFPRDRYDHTCDLVDFEKLKDKRVLILGGRQSAFEWAAL
                     MRESGVGAVDLCYRHDTPSFTTSDWSWVNALVGRMADDPGWYRRLGPDEKQQLNERFW
                     AEGRLKLEPWLARRIDYPNVRLHPHTEVRGADADSSGIVVSLDDGDVLTVDHVVFATG
                     YDVDLARVPFLANGLLSRLDTQDGFPLLDTHFQTSVPGLYITSLPATRDFGAFLGFTV
                     SVRAQATIIGRHITEEYRSAVAFHSPFRTAAAP"
BASE COUNT         1135 a         1586 c         1602 g         1133 t
ORIGIN      
        1 aggatcaacc ggagtattat cctccaactc gaaccggcat gcgtggcagc catccgggat
       61 cgttcgagga tgcccacgcg gtcagggaca atccgaatat tgttgctgat gccgtggata
      121 ccggtgagcg ctttgacctg attgttgctg gcgccggcat cagcgggctt gctgcagctc
      181 acttttatcg caaagcggcg ggcccccgcg caaagatcct cctgctggat aatcatgatg
      241 attttggcgg ccacgccaaa cgcaatgaat tccatgtgaa cggcaaagtc ctgatcatga
      301 acggcggaac cgccgggatt tccagtccaa ccccatacag tcctgttgcc gacggcctgc
      361 tcaagcatct cggcattcat ccgactgagc tcgcggaggc gacgtacgat cacgatttct
      421 acagcaaaaa caacctgggt tccgcagtct tctttgacaa ggaaaccttc ggcgacgata
      481 agctcgttca acaatccggc ggccgtcaat cgacagaaga gacagcagca ttcctggcgc
      541 gagcgccgct aagtgaggcc gtccgcaaag acatcctcgc aattgaaacc ggcaggaacg
      601 actacatgcc cggcatgtcg gcggatgcga agaaggatcg cttgtcgcgc atgagctacg
      661 ccgattacct gctgaaggtt gtaaaagccg atccggccgt gatttcctat tatcttcacg
      721 acaccgacgg gctcgggggt tgcggaatcg atgctgtttc ggcgctcgat tgctgggggg
      781 tcggattccc cggcttccaa ggcctgaacc tgccgaggac agccacagcc cgcatgggct
      841 atacgccggg aaaagagctc gaaactggcg gttcctaccg tttcgctttt ccagatggca
      901 acgcgtcggt tgcacgcctg ctggtgcgcg atctaattcc cgacgccgtg ccgggcacga
      961 ctgtcgagga ttccatcgcc gctgaagtga aatacggaaa gctggacagc aacgacaact
     1021 ctgtgcacat ccggctttca agcacagtgg cgcatgcggt caatgccagg gatggtggtg
     1081 tgaaggtcac gtacctgcgt gacggcaagg catattgtgt cagaggtaaa cacagcgtac
     1141 tggcctgctg gaatatgatg attccgttta tctgtcccga actgccagag gagcagaaga
     1201 gggcgttgca ctatctcgtt aagacgccgc ttgtctatac gtcagtggct ctgaccaact
     1261 ggcgcgcatt tgcaaactta ggtgtgtcgc gcgttcgttg ccccggcagc taccatacga
     1321 gcttcagcct ggattccgtc atggacatcg gcgactacca tagtgcgaag tcgccggacg
     1381 aaccggttat ggttcatatg actcgcgcgc cggcacgccc cggccttggt gaacgggaac
     1441 aacataaggc aggtcgtgcg gaacttctga ccacaccatt ctcaaccttc gagcgccata
     1501 tccgcagcca gatgaatcgg gccctcggct ctggtggctt tgacgcggca aacgacatcg
     1561 ccggcatcgc tgtgaaccgt tggccgcacg gatatgctcc tgaatacaat tcgcttgttg
     1621 atccggacga agggctgcca ttgttgacgg cacgcgcacg cgcgggagcc attgctatcg
     1681 cgaatgcgga cagcggcggt ggtgcctaca ccgacgtcgc gatcgaccag gcacatcgcg
     1741 ccgtgatgga acttctaacg gcatagaagt tggaggacct tcgttgcact cgcacagaga
     1801 aatgatatgt cttccggact gaatatttca cgtcgggatt tcctgaacgg ggtcgccatg
     1861 gcgactgcgg ccggcagcat gctgtcaccc atggacctgc tcgccgcgca ggtttccgcc
     1921 gattattacc caccgctcag gacgggtatg cgtggcagcc atcctggttc gttcgaggtg
     1981 gcgcacgggg tttcctgggc cgggcacaag tacccaacgc cgaaacaaca gactgacgac
     2041 gtgtacgaca tgatcgttgt cggtggcggc atcagcggcc tgtcctcggc gcttttttat
     2101 caccagcgcg cgggtgtgga caaaaatgtg ctggtgctcg acaaccacga cgacttcggc
     2161 gggcacgcca agcgcaacga gttcaatgtt aatggccaga cgctgattgg gtatggtggc
     2221 agccagtcga tcgacggccc gggaaacttc tccgcggcat ccagccgcat gttgaaagac
     2281 gtcggcatcg tcaccgagcg cttctacgac tatttcgacc aggattacat gagaaaccga
     2341 aatcttcggc gcgccattta cttcagttcc gaagtatatg ggcgcgaggt cactgcgccg
     2401 aacatacttc gtggcgattt cggggatgat ccaaaacaac tcgacgcaat tgtcgcgcag
     2461 tacccgatat cggacgagtc caaggccgca ctgctgcgcc tgtggcgcga cgagaccgat
     2521 catcggccgg acctcagccg cagcgagaag atcgattttc ttggcagcat cgcctacacc
     2581 aaatatctgc gtgagtacgt cggcatgccg cgccaggtga ccgatctcct ccgtgacacg
     2641 attctggggc tatggggtgt cggctgggat tcgctgtccg cgctcgaagc ttaccgctcc
     2701 ggaatgccgg ctttgcagca catgggcctc ggcgagctcg ctgagcaagg ccacgaaaga
     2761 gacgagccgt acattttcca tttcccggac ggcaatgccg gcctggcgcg ctcactcgtt
     2821 cgcagcttgc tgcccaatgc cgtgccaggc agcacgatgg aggacctcgt gatgtcgcgc
     2881 atcgactaca gccttctgga tcgtaggaag agcccgacgc gaatccgcct gaatagcacg
     2941 gcagtgaact tacatcacac gcccgaccag aagcatgtcg atgtcaccta cgtaaccgat
     3001 ggcgatgctt atcgtgttcg cgcgaagcac gtggtattcg ccggttacaa cgcgatgctt
     3061 ccatatatat gcggcgaggt acccccggcg cagaaggaag ctatcgcgta cgccgaaaag
     3121 gtgcccctgg tgtacatgtc catcgcggtg cgtaactggc atgcgctcgc agagatggga
     3181 tacagcagcg tcttcattcc gcggtcaaaa tacatatact cgtttggcct ggattttccg
     3241 gtcagcatgg gcgattaccg gttcacacag gatccccaac aacccacggt tttgcacggt
     3301 tcctgggtgc cgaacgtgcc cgagcagggt ctctctgcgc gggagcaaca tgtggccggt
     3361 cgtcgccagc tctacgagtt gagcttcgat gacttcgaac aggatattgt cgaaaagatg
     3421 agcggttccc tggctccggg tggtttcgat gcggaacgtg acatcgccgc gatcaccgtc
     3481 aatcgatggc cgcacggcta tgcctatgaa tacaacgatc tgagtgatcc ggagggcttc
     3541 actccgcaga acggtcccca cgtagctggc cgcgcccaaa tcggccgcat ttcgattgcc
     3601 aactcggatg cgtcggctta cgcatatgtg aatggtgcga tcgacgccgc cgaccgggcg
     3661 gtcaacgagc agatgcagac agcgtaatca taggctttat ttgcaaacgc ccgcgtttgc
     3721 atgtcgcgcc gccatctggt tctgatatta gcgaggagat tgacgttctc gtcgatcgaa
     3781 ctgggggacg cacccaaccc tgctcgcgac gaattcagcg caaggagccg agatgttcgc
     3841 gacggttcac ggcgctacac cacgcacaat cgcatttacc agcgacacaa tgtgtaattt
     3901 gtagcgcgaa tcatcggcaa acaatgtcgg ccggaacgcc gacacagcgt gcaggccttc
     3961 gaggacagca atagcggtca atacgcgccg ttccagcttt cgtggatgaa cttttagttt
     4021 caggcggcgt gcgagaccga gcatggttgt cacaaacgcc tcgtaaatct catctcgctt
     4081 cgtcgcgatg cgatcgttgg attccgctac ggtaatgatt tgcgaaaaga atatacgcac
     4141 tgttggatcc tggtactcgt caatccagcg gctgacgtaa tcgtcaaatg cggcctgtgg
     4201 atcgtcgtcg gcgatatgcg attctggatg atgtcgccgg tagtactcgg cgagttcata
     4261 atcgattacc gccagccaca gtgactcctt ggtgggaaaa tagtagctga cattaccgtc
     4321 acttatcccc agcgagcttg ctacgctgcg cttgttcaga gccaggaagc cgtgttcgac
     4381 gagtatgcca gacgccgccg cgaggatttc gttgacgcgt cggctccctt tgctggtgag
     4441 ctcggttcga gcgacgccgg taggtttttc cttgctcatc tggttttgcc cggtgtttcg
     4501 gaccgtttgg agctggtcgt ctgctctcag ggggcggcag ccgtcctgaa aggcgaatga
     4561 aacgcgacgg cggaacgata ttcttctgtt atatgccggc cgataatcgt cgcctgggcg
     4621 cgaaccgaga cggtaaagcc gagaaacgct ccgaagtccc gggttgcagg gagactggtg
     4681 atgtacaggc cgggtacgct tgtctggaag tgggtgtcaa gcaacggaaa gccgtcctgc
     4741 gtgtccagtc tcgacagcaa tccgttggcc aggaacggca cccgtgccag gtcgacgtcg
     4801 tagcccgtcg caaacaccac atggtcgacc gtcaatacgt cgccgtcgtc cagtgacacg
     4861 acaatcccgg agctgtcggc gtcggctccc ctcacctctg tgtgggggtg gagcctgacg
     4921 ttcgggtagt cgatacgcct ggcgagccac ggttcgagct tgaggcggcc ttcggcccaa
     4981 aatcgctcgt tgagttgctg cttttcgtcc gggccaagcc ggcggtacca gcccgggtcg
     5041 tcggccatgc ggccgacgag cgcattcacc cagctccagt cggaagtggt gaacgacggc
     5101 gtatcatgcc gatagcagag atcgacagca cccacgccgg attcccgcat cagtgccgcc
     5161 cactcgaaag cgctctgccg gccgccaagg atgaggaccc gcttatcctt tagtttttcg
     5221 aagtcgacca ggtcgcacgt atgatcgtac cggtcgcgcg gaaacaaact cgccagttcc
     5281 agtggcacgt tggtaaagct tccaaagccg gttgccatca cgacgaagcg gctgcggata
     5341 cgctcgccgc tttcggtgac cgccaccaga tccgttccgt ccgtgttaag ggaactcacc
     5401 gtcgcttgca ggctgcgcag gtccttttca tgctggaacc agcccgtata gtcgag
//
LOCUS       DASUOM010000468         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1382677,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000468 DASUOM010000000
VERSION     DASUOM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382677"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VFY27_09715"
     CDS             complement(<1..2053)
                     /locus_tag="VFY27_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX5765833.1"
                     /translation="MITSAAGKFRALALLSATVLLHACAPDDGGDLSAANAQATSDTA
                     VHQAEVAVGQLDEIMARGEEVYLANCGTCHQPSGEGLGNAFPPLAASDYLKQHERKEL
                     LKTALFGLSGPIVVNGKPYNGVMPSMGYLTDQQLADALTYVFNSWGNDLAAVSADEVA
                     SLRLEEGELARAGEQPHLGTTEAEIRYRGTPSPIDAGATREVQTSEGPQLTETEYAMA
                     TQIYFERCAGCHGVLRKGATGKPLTPDVTRPKGHEYLEALITYGSPAGMPNWGSSGEL
                     TEEQIDVMARFLQQEPPEPPEYGMAEMKDSWKVMVQPEDRPDAPRHERNIENFFAVTL
                     RDAGQVAIIDGDTKEVISILPTGYAVHISRPSASGRYVYTIGRDAKVDMIDLWMDPPA
                     IVAEIKIGLEARSVETSKFKGFEDQLAIAGAYWPPQFVLMRGDTLEPLKIVSTRGMTV
                     DTQEYHPEPRVAAIVASHEHPEFIINVKETGHILLVNYRDIDNLTVTDIEAARFLHDG
                     GWDRSKRYFMTAANQSDKIAVVDSRDRKLAALVDVEKIPHPGRGANIDDPAHGPAWIT
                     SALGNANVTFLGTDPAGNPDNAWKVVRVLQGQGGGSLFVKSHPRSNNLWVDSPLNPDE
                     AISQSVAVFAIDNLDAGYDVLPIAEWAELGEGPKRVVQPEYNQAGDEVWFSVWSGKEE
                     ES"
     gene            complement(2083..>3379)
                     /locus_tag="VFY27_09720"
     CDS             complement(2083..>3379)
                     /locus_tag="VFY27_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765834.1"
                     /translation="GAPGGLVDAGVWRASFGRTRVDQHTHDERALAARPVAVERRFLF
                     EQSVRSLELNLQKNLQFAGGSHGIGAGLEHRRKRTEELRDGTEKGLEDGIVTSTILGE
                     TFPLRDFPVSETTEWGAWIEDAITFGDVTVIAALRADRYELSVRDDAIFAQANPAIEP
                     VALTEADLSPKLGLVYRLTDATDVYVQYARGFRAPPFEDANIGLDIPLFNIRAIPNPD
                     LQSETSDGLDIGLRWTAQDSSLHLGVFSTRYSDFIETRVRLGLDPLSGRILFQSRNLS
                     EARIHGLEAGWYSRLPGTLRAFTIEGSLYKARGENRDNGEPLNSVGPAQAVLGVSWDG
                     GERAPQVRLKATLSEGWSERDETAGELFEPAGYAIFDLYVTQRVGVHTTLRAGLMNLT
                     DRTWWNWSGVRGLAPDDPVLAAIAQPGRSLTIGVNMKWQ"
BASE COUNT          579 a         1089 c         1009 g          702 t
ORIGIN      
        1 cagactcctc ttccttgccg ctccagaccg agaaccacac ttcatcgccg gcctggttgt
       61 attcgggctg aaccacacgc ttcgggcctt cacccagttc ggcccattcg gcgatcggca
      121 ggacgtcgta acctgcatcg aggttgtcga ttgcgaaaac tgcaacgctt tggctgatgg
      181 cttcatcggg attgagcggc gagtcgaccc acaggttatt cgacctgggg tgcgatttca
      241 cgaacagcga accgcctccc tggccctgca gcacgcgcac cactttccat gcgttatccg
      301 ggttgcccgc cggatcggtg ccgaggaacg tgacattcgc gttgccgagg gcgctggtga
      361 tccaggccgg gccgtgggcc ggatcgtcaa tgttggcgcc gcgtccgggg tgcgggatct
      421 tttcgacgtc gaccagcgcc gccagcttgc gatccctgga gtcaacaacg gcgatcttgt
      481 ctgactgatt ggctgcagtc atgaagtagc gcttgctcct gtcccaaccg ccatcgtgca
      541 ggaacctcgc cgcttcaatg tcggttacag tcagattgtc gatatcccgg taattgacca
      601 gcaggatatg acctgtctcc ttcacgttga tgatgaactc cggatgctcg tgtgaggcca
      661 cgatggccgc gacacgcggt tcggggtgat actcctgcgt gtcgacggtc atgccgcgag
      721 tagacacgat cttcagcggc tcgagagtgt cgccgcgcat gagtacgaac tggggcggcc
      781 aatacgctcc ggcgatcgca agctggtctt cgaaaccctt gaacttcgag gtttcgaccg
      841 accgggcttc caggccgatc ttgatttccg caacgatcgc cggtggatcc atccagagat
      901 cgatcatgtc gaccttcgcg tcgcggccaa tggtgtaaac atagcgtccg gatgctgatg
      961 gccgcgaaat gtgaacggca tagcccgtcg ggaggatgct gatcacctcc tttgtgtcgc
     1021 catcgatgat agcgacctgt cccgcgtctc ttaacgttac cgcgaagaag ttttcaatgt
     1081 tcctttcgtg acgaggcgcg tcgggcctgt cttcgggctg aaccatcacc ttccagctat
     1141 ctttcatttc cgccatgcca tattccggcg gctctggcgg ttcctgctgc agaaaacgcg
     1201 ccatcacgtc aatctgttcc tcggtcagct cgccggaact gccccagtta ggcataccgg
     1261 cgggtgaccc gtaggtaatc agtgcttcaa ggtattcatg ccccttcggt cgtgtgacat
     1321 ccggcgtcag cggcttgccg gtagcgcctt ttcgcagcac accgtggcaa cccgcgcacc
     1381 gctcgaaata aatctgcgtc gccatggcgt actcggtttc agtaagctgc ggcccttcgg
     1441 aggtctgcac ctcccgcgtc gcccctgcgt cgatcggcga tggtgttcca cggtagcgaa
     1501 tttccgcctc ggtcgttccc aggtgcggtt gttccccggc gcgagcgagc tcgccttctt
     1561 ccagtcgcag tgacgccact tcgtcggcac tgacggcagc gaggtcgttg ccccatgagt
     1621 tgaagacgta ggtgagcgcg tccgccagct gctggtcggt gagataaccc atgctcggca
     1681 tcacgccgtt gtacggcttg ccgttgacca cgatagggcc cgacaatccg aacagcgccg
     1741 tcttcagcag ttctttccgc tcgtgctgtt tcaggtaatc ggaggctgcg agcggcggga
     1801 acgcgttgcc gagtccttca ccggaaggct ggtgacaggt accgcaattc gccaggtaaa
     1861 cctcctcgcc gcgcgccatg atttcatcca gctgcccgac tgccacctcc gcctgatgga
     1921 cggcggtgtc gctggtggct tgcgcgttcg ccgctgaaag gtcgccgccg tcgtcggggg
     1981 cgcaggcgtg cagcaacacg gttgcagaga gcagtgccag tgcacgaaac ttgccggctg
     2041 cggatgtgat catctcgttt actcctctcg aaaattgcgg cttcattgcc atttcatgtt
     2101 cacaccgatg gtaaggctgc ggcccggctg cgcaatcgcg gcaagcacgg gatcgtcagg
     2161 tgccagtccg cgtactccgg accagttcca ccaggtacgg tcagtgagat tcatgagccc
     2221 tgcgcgaagc gtcgtgtgta cgccgacgcg ctgcgtcacg tacaaatcga aaatcgcgta
     2281 gcctgccggc tcaaacagtt cccctgcggt ctcgtcacgc tcggaccagc cttccgacag
     2341 cgtggctttg agccgcacct gcggcgcgcg ctcccctccg tcccagctca ctcccagcac
     2401 agcctgcgcc gggccgaccg agttcaatgg ctcgccgttg tcccggttct cgccacgtgc
     2461 cttgtacagc gagccctcga tcgtgaatgc tcgcagtgtg ccgggcaggc gtgagtacca
     2521 gccggcctcc aggccgtgga tgcgcgcctc gctcaggttc cgcgactgga aaagaatgcg
     2581 accgctcagc gggtcgagcc ccagtcttac cctggtctcg atgaaatcgc tgtagcgggt
     2641 gctgaatacg cccaggtgca gactcgaatc ttgcgccgtc cagcgcagac cgatatccaa
     2701 cccgtccgac gtctcggatt gaaggtccgg gtttgggatg gccctgatgt tgaacagggg
     2761 tatgtcgagc ccgatgttgg catcctcgaa cggtggagcg cggaagccac gcgcatactg
     2821 cacgtagaca tctgtcgcgt cggtgaggcg atacacgagg cccagcttcg gcgacaggtc
     2881 ggcctccgtc aacgccacgg gttcgatcgc ggggttggcc tgcgcgaaga tcgcatcatc
     2941 acgcaccgac aattcatagc gatccgcgcg cagcgcggcg ataacggtca cgtcgccgaa
     3001 cgtgattgcg tcttctatcc aggcgcccca ttcggtcgtc tcgctgaccg gaaaatcccg
     3061 cagtgggaag gtctcgccaa ggatggtgct ggtcacgatg ccgtcctcga gacccttctc
     3121 cgtaccatcg cgcagctctt cggttcgctt gcggcgatgc tccaggcctg cgccgatccc
     3181 gtgacttccg ccagcaaact gcagattctt ctgcaggttg agctcgaggc tgcgcacgct
     3241 ttgctcgaac aggaagcggc gctcgactgc caccggccgc gccgcgagtg cccgctcgtc
     3301 gtgagtatgc tgatccaccc gcgttctgcc gaaagacgcg cgccacaccc cggcgtcgac
     3361 caggccaccg ggcgcaccg
//
LOCUS       DASUOM010000469         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_693240,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000469 DASUOM010000000
VERSION     DASUOM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693240"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFY27_09725"
     CDS             <1..1324
                     /locus_tag="VFY27_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765835.1"
                     /translation="ESRVTFSTEGSIDREFSTLELATFAMTQGSAMDLSGNLRGGMRP
                     GNAGPALSGAVELRESSVSAIYTGLLQVFLAGTAAGDLATDGTVAGIVTLDNNELSGL
                     DLSLGQVNIDDNQGRFAVYGLNGRLHWPGPGGDAADAEPSSLRWDSASAWNIPLEAAA
                     IEAKLGGDDFHLSMPLRVPTMGGALLINRLVLNDFGTEGASGVLDAELEPIELGQLTG
                     AFGWPAFSGSLAGELPLLQYESGVVTLGGALSARAFDGDIEFENLRLEEPFGLVPRLY
                     GDLRLRQLDLERLTDTFSFGLIQGRLSGEVAGLEMIGWEPVAMDLHLYTPPNDKSRRR
                     ISQRAVENLASVGGGGAAAALSSGFMKFFEEFSYDRIGIRCVLADGTCRMSGAGPAGQ
                     SDLGNGYYIVKGSGLPRIDVVGYRHQVSFSALVRQLEAITASGAPVVQ"
     gene            1490..2092
                     /locus_tag="VFY27_09730"
     CDS             1490..2092
                     /locus_tag="VFY27_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HEX5765836.1"
                     /translation="MKKVALAFAGLLAVFAVACVTINVYFPEAAAEQAADRIIDKVRG
                     EPTDESGAYLVAPVTPVEPLLVAAARQLLSFVIADANAQGTVDFDKPSPKKTALENSL
                     ANRFPQLEPFFDSGAIGVNETGTIEIRDRNLVPLKDRNSLLQLVNAQNSDWDALYAEI
                     ANLNGHPEWVDSIRRTFASRWVAKADAGWWYKEGGSWQQK"
     gene            complement(2117..2548)
                     /locus_tag="VFY27_09735"
     CDS             complement(2117..2548)
                     /locus_tag="VFY27_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765837.1"
                     /translation="MSERLKGRICLFALCFLAGMAAAQQEEALEFKGSSSTTTPKFTV
                     EAPWILDWRVFSDFPQSVSIEIALLDGTTGMHDGLVLQTKEMGNGVKLFNEGGTYQLR
                     IDSDLARWQILVKELTKEEAEQYTPKETGFQRDSPFRRRDN"
BASE COUNT          513 a          794 c          844 g          510 t
ORIGIN      
        1 tgaaagccgg gtgacgttca gcacagaggg cagcattgac cgggagttct caaccctgga
       61 acttgctacg ttcgccatga cacagggctc tgccatggat ttgagcggca acctgcgtgg
      121 cggcatgaga ccggggaatg ccggacctgc gctcagtggc gcggtcgaac tgcgggaatc
      181 ttcggtgagc gccatttata ccggcttgtt gcaggtgttc ctggccggaa ccgccgccgg
      241 ggatttggcg accgatggta cggttgccgg tatcgtcact cttgacaaca acgagctgtc
      301 cggactcgat ctttcgctcg ggcaagtgaa catcgacgac aaccagggac gctttgcggt
      361 gtacggcctc aacgggcggc tccactggcc gggtcccggc ggcgacgcag ccgacgccga
      421 acccagcagc ctgcgctggg acagcgccag tgcatggaac atccccctcg aagctgccgc
      481 gatcgaagcg aagctcggcg gtgacgactt tcacctttcg atgccgttgc gcgtgccgac
      541 gatggggggc gcgctcctca tcaatcgcct ggtgctcaac gacttcggca cggaaggcgc
      601 cagcggcgta ctcgacgccg agctggaacc catcgaactt ggacagttga cgggtgcgtt
      661 cgggtggcct gcattctccg gttcgctcgc cggcgagctg cccctcctcc agtacgagag
      721 cggcgtggta acactgggcg gcgccctttc cgcacgggcc ttcgatggcg acatcgaatt
      781 cgagaacctg cggctggagg agcccttcgg gctggttccg cgcctttacg gcgacctgcg
      841 gctgcggcaa ctcgacctcg aacggctgac cgacacattc tctttcggcc tcattcaggg
      901 acggttaagc ggcgaagttg caggcttgga gatgattgga tgggagcccg ttgccatgga
      961 cctgcacctc tacaccccgc cgaacgacaa gtcgcgccgg cggataagcc agcgagcggt
     1021 cgagaacctc gctagcgtcg gcggcggcgg tgccgcggct gcgctatcgt cgggcttcat
     1081 gaaatttttc gaggagttct cctacgacag gataggcatc cgctgcgttc tcgcagatgg
     1141 cacctgcagg atgagcggag ccggcccggc gggacaaagc gacctgggca acggctacta
     1201 catcgtgaaa ggcagcggac tgccgcgcat cgatgtagtt ggctaccggc atcaggtaag
     1261 ctttagtgcg ctcgtaaggc agctggaagc gatcaccgca agcggtgcgc cggtggtgca
     1321 gtgaaatcgc agccgtagga ggccacgtta aaggtaagct cgacgaatcc aattcgtacc
     1381 gtcgcggcgg acggcgactg cgggcgagtt cgctaacgct catggaaccc ggccgcacgc
     1441 ggctccgcag gttcaaaaac acttcaataa cgaacgggga gtttcaacga tgaagaaagt
     1501 ggcgctggcc ttcgcaggtt tgctggcggt ttttgcagtg gcgtgcgtaa ccatcaacgt
     1561 ttatttcccg gaagccgccg ccgagcaggc agccgaccgc atcatcgaca aggtgcgggg
     1621 cgagccgacg gatgaaagcg gcgcgtatct cgttgctccg gtcacaccag tggaaccact
     1681 gctggttgcc gctgccaggc aattgttgag cttcgtgatt gcggatgcaa atgcgcaggg
     1741 cacggtcgac ttcgacaagc catcgcctaa aaagacggca ctcgagaact cgctggcgaa
     1801 ccgtttcccg cagctggagc cattcttcga ttccggcgcg ataggcgtga acgaaaccgg
     1861 gaccatcgaa attcgcgatc gcaacctggt gccgctcaag gaccggaaca gcctgctaca
     1921 actggtgaac gcccagaaca gcgactggga tgcgctgtat gcggaaattg cgaaccttaa
     1981 cggtcatccc gaatgggtcg acagcatacg ccgcacgttc gccagccgct gggtagccaa
     2041 ggcggacgcc ggatggtggt acaaggaagg cggcagctgg cagcagaagt agccgcggcc
     2101 ggcgcctgca gtttgatcag ttatcccgcc gccggaaggg gctgtctcgc tggaagccgg
     2161 tttccttcgg cgtatactgc tcggcttctt ctttcgtcag ttccttcacc aggatctgcc
     2221 agcgcgcgag gtcggagtcg atgcgcaact ggtaggtgcc gccctcgttg aacaacttga
     2281 cgccgttgcc catttctttc gtctgcagca cgaggccgtc atgcatgccg gtagtgccgt
     2341 ccagcagcgc gatctcgatc gataccgact ggggaaaatc gctgaacacg cgccagtcga
     2401 gtatccatgg tgcctccacg gtgaacttcg gcgtcgtggt gctgctcgat cctttgaatt
     2461 caagcgcttc ttcctgctgc gccgctgcca tgcccgccag gaaacagagc gcgaacaggc
     2521 agatcctgcc tttcagacgt tcagacatga ctgactccag gtgtcgatac gtttgagttt
     2581 cactataccc aatgtggaac gatatcgtat tagtcgagcc aggtttccgc ggagttccct
     2641 gccttcgatc gtcgccgggg g
//
LOCUS       DASUOM010000470         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_658171,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000470 DASUOM010000000
VERSION     DASUOM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658171"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="waaF"
                     /locus_tag="VFY27_09740"
     CDS             <1..846
                     /gene="waaF"
                     /locus_tag="VFY27_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX5765838.1"
                     /translation="QLGWTRRRALGKTLRDRRYSQAFVLPNAMKSALVPWFANVPLRT
                     GYRGEWRYGLLNDLREKDPRKTIPPARRWLALGYRADAELPPVTPPPRLHPDAGNRDR
                     IVQFLGLRSSAPVVALVPGGEHGSSKRWPVEYFGELARALAKQGIAVWVLGSSREMPL
                     GNSIATEGGKAVVNLCGRTMLPDVIDLLGSAAGVVCNDSGLMHVAAALGVFVVALYGP
                     TTPTFTPPMTDNCRVLYLDLPCSPCYSGACPLEHHNCLRQISPGAVLEALLPAIPSGP
                     GAVSA"
     gene            complement(906..>2607)
                     /locus_tag="VFY27_09745"
     CDS             complement(906..>2607)
                     /locus_tag="VFY27_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765839.1"
                     /translation="DRYALNPDNKPESELLRGALVWLLIAAGIALAARLAHTFQDYVL
                     AMVVQRFGMQIFNDGLRHTLRLSFQEFEDRSSGETMAMLQKVRTDTERFIFAFVNILF
                     SSVVGFGFLLWYGITTHWLLIPVFLVGVLLLGGLTSVLSQKMKAIQRMVVRETAGMAG
                     SITESLRNIELVKSLGLTYPEIRRLQVHTRKIFELEMDKSKKVRTLSFFQGTLINTLR
                     QSILFILLWLIFRETLTTGELIATQFILNMVFVPLQNLGRIILNYREAQASMQLFDEL
                     MQKPVEKRPEDPVELGQLSDLRFDDVVFKHRTATENAVDHLSFDVKLGQTIAFVGPSG
                     SGKSTLVKLLVGLYTPISGTIYINGVPVTELRYNPVRRQIGFVTQDPQLFSGTIRENL
                     LLVKPDATDEQMHEALRSASATELMARSGKGLDTRLGESGLRVSGGERQRLSIARALI
                     RGPRLFIFDEATSALDSITEQEITTAVRGVSGSREHMVILIAHRLSTILHADTIYVLE
                     KGRIVESGTHGSLLEQKGLYYAMWRQQIGERDARLFRSPGGETRFKEEEEPELVDTPD
                     "
BASE COUNT          489 a          830 c          769 g          519 t
ORIGIN      
        1 caactgggtt ggacacggcg ccgggcgctg gggaagactt tgcgcgaccg gcgctattcg
       61 caggcattcg tattgcccaa tgcaatgaag agcgcgcttg tgccgtggtt tgcgaacgtg
      121 ccgctccgta ccggttaccg cggcgaatgg cgctatggct tgctgaacga cttgcgggag
      181 aaagacccgc ggaagacgat accgccagcg cgcaggtggc tggcgctggg atatcgcgcc
      241 gatgcagagc tacccccggt cacgccgcct cccaggttgc acccggacgc cggcaaccgc
      301 gaccgcatcg tgcaattttt gggactgcgg agttccgcgc cggtggtggc actcgtgccg
      361 ggaggcgagc acggatcctc caagcgctgg cccgttgagt acttcggcga acttgcccgg
      421 gccctggcga aacagggaat tgccgtgtgg gtcctgggct catcccgcga aatgccgctc
      481 gggaacagca tcgcgaccga aggcggaaaa gcggtagtca acctgtgcgg ccgcacgatg
      541 ttgcccgacg tcatcgacct gctcggatcg gctgcgggcg tcgtgtgcaa cgactccggc
      601 ctgatgcatg tggccgctgc gctcggcgtg ttcgttgttg ccttgtacgg acctacaacg
      661 ccaaccttca cgccaccgat gaccgacaac tgtcgtgtcc tgtacctcga tctgccctgc
      721 agtccgtgtt actccggggc atgtccgctc gagcatcaca actgcctgcg acaaatcagt
      781 cccggcgccg ttctcgaggc cttgttgccc gctattccat ccggacccgg tgcggtcagc
      841 gcctgacgtc tgcccggctg gaagaaatcg ctgcaggagc ccgtcccacc ggcgacacgc
      901 gtcaactaat caggagtatc gacaagttcg ggttcttctt cttccttgaa acgcgtctcc
      961 ccacccggcg accgaaacag ccgcgcatcg cgttcgccga tctgctgccg ccacatcgcg
     1021 taatacaagc ccttctgctc aagcagcgac ccgtgggtgc cggactccac aatccttccc
     1081 ttctcgagta catagatggt atccgcgtgc aggatggtac tgagccggtg cgcgatcagg
     1141 atcaccatgt gctccctgct gccggatacg cctcgcaccg cggtcgttat ctcttgctcc
     1201 gtaatggaat cgagcgccga cgttgcctca tcgaagatga agagccgcgg cccgcgtatc
     1261 aatgcgcggg ctatcgacag gcgctggcgc tcgcctcccg aaacgcgcag tccgctttcg
     1321 ccgagccgcg tgtccagccc tttgcccgac cgagccatca attccgtcgc cgaagcacta
     1381 cgaagcgcct cgtgcatttg ctcgtccgtg gcgtccggct tgaccagcaa caggttctcg
     1441 cgaatcgtcc ccgaaaacag ctgcggatcc tgggttacaa aaccgatctg ccgacgcacc
     1501 gggttgtacc gcagctcggt taccggcacc ccgtttatgt agatcgtgcc ggagatgggc
     1561 gtatacagtc ccaccagcag tttcaccaac gtcgactttc cggatccgga cggtcccacg
     1621 aatgcgatcg tttggcccag tttcacgtcg aagctcagat gatcgacggc gttttctgtc
     1681 gcggtgcggt gcttgaacac cacgtcatcg aagcgaaggt cggagagctg gcccagctcc
     1741 acgggatcct ccggccgctt ctcgaccggt ttttgcatca gttcgtcgaa caactgcatc
     1801 gaagcctgcg cctcccggta attcaggatg attctcccga ggttttgcag cggcacgaat
     1861 accatgttga gtatgaactg tgtcgcgatg agctctcccg ttgtaagggt ctcccggaag
     1921 atcagccaca acaggatgaa gaggatcgac tgcctgagcg tgttgatcaa agtgccctgg
     1981 aagaacgaca gtgtgcgcac tttcttcgac ttgtccattt cgagctcgaa aatcttccgc
     2041 gtgtgaacct gcagccggcg gatctccggg taagtgagcc ccaggctttt aacgagttct
     2101 atgttgcgca gtgattcggt gatcgagccc gccatgcccg ccgtttcccg cacgaccatg
     2161 cgctggatcg ccttcatctt ctggctcagc acgctcgtga gcccgccaag cagcaacacg
     2221 ccgacgagaa acaccggaat cagcagccag tgcgtggtta ttccgtacca cagcaggaag
     2281 ccgaacccga cgacggaaga aaagaggatg ttgacgaacg cgaagatgaa cctttccgta
     2341 tccgtcctga ccttttgcag cattgccatg gtctcgccgc tgctgcgatc ttcgaactcc
     2401 tggaacgaca ggcgaagtgt gtggcgcagg ccgtcgttga agatctgcat gccgaaccgc
     2461 tgcacaacca ttgccaggac gtagtcctgg aacgtgtgcg cgagtcgtgc cgcgagcgcg
     2521 atgccggcgg caatcagcag ccagaccagg gcgccgcgaa gaagctcgct ctccggcttg
     2581 ttgtccgggt tcagcgcata gcggtcg
//
LOCUS       DASUOM010000471         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_614998,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000471 DASUOM010000000
VERSION     DASUOM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614998"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFY27_09750"
     CDS             <1..188
                     /locus_tag="VFY27_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765840.1"
                     /translation="ASVLGRVLTGTTAGGLIDTMGYVNFYLLTTVLALPGILLFWFMI
                     RKGLIDTALGTAGKVTR"
     gene            complement(185..1012)
                     /locus_tag="VFY27_09755"
     CDS             complement(185..1012)
                     /locus_tag="VFY27_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5765841.1"
                     /translation="MKLFRVITVNVNGIRAAARKGFFDWLSTQTPDVVCIQETKAQHH
                     QLGDPCFAPDGYHCYYEDAIRKGYSGVAIYSRHEPDAIVRGFGVPEFDDEGRYLEIRL
                     GQLSIVSVYLPSGSAGELRQASKFRFLDAFVPHLSGLRNSGREYILCGDWNIAHKEID
                     LKNWRSNRNNSGFLPEERQWLDELFGTHEFVDAFRTINNAAEQYTWWSNRGRAWDKNV
                     GWRLDYQIVTPGLRDKILSAHIYKEQRFSDHAPLIMDYRLDLTRTGFASAVAARQAG"
     gene            1195..1857
                     /gene="pyrE"
                     /locus_tag="VFY27_09760"
     CDS             1195..1857
                     /gene="pyrE"
                     /locus_tag="VFY27_09760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5765842.1"
                     /translation="MRAYKQEFLDLALELGVLRFGEFTLKSGRVSPYFFNTGLFNTGY
                     AAAKLGRYYAAAIAASRVEYDMLFGPAYKGIPLVALAAAALAEHHDVDVPYTFNRKEA
                     KKHGEGGVVVGAPLSGKVLIVDDVITAGTAAREAQQIITGAGAEVAGLVISLDREEVG
                     QDSRSAVQELEQTLKVPVISIVRLEDLIDLLEESDEFGEYLEPVVRYRDTYGINAGLV
                     ST"
     gene            2007..>2332
                     /locus_tag="VFY27_09765"
     CDS             2007..>2332
                     /locus_tag="VFY27_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5765843.1"
                     /translation="MRNLLLTAASLLLLSQGALGAQDQKLYRWVDQEGNVHFGDSIPA
                     EYAEVEKQVVNEHGVTLDVLRGKKTAEEIAAEKKAEELKAERELRRRQDQALLATYLS
                     VDEIVM"
BASE COUNT          494 a          660 c          709 g          469 t
ORIGIN      
        1 cggcgagcgt cctcggccgc gtgctgaccg gtacgaccgc cggcggcctc attgacacca
       61 tgggctacgt gaacttttat ctgctcacca cggtgctggc gttgccgggc atcctgctgt
      121 tctggttcat gatccgaaaa ggcctcattg ataccgcgct cggaacagca gggaaagtaa
      181 cgcgttagcc cgcctgcctg gcggcaacgg ccgacgcgaa acccgtgcgg gtaaggtcga
      241 gacggtaatc catgatcaat ggcgcgtggt cggagaatcg ctgctccttg taaatgtgcg
      301 cggacagtat tttgtcacgc aggcccggcg tcacgatctg gtagtcgaga cgccacccga
      361 cgttcttgtc ccatgcgcgg ccacggttcg accaccaggt gtattgctct gctgcattgt
      421 tgatggtccg gaacgcgtcc acgaattcat gggtgccgaa aagttcgtcc agccattgac
      481 gctcctccgg caggaagccg gagttgttcc ggttggagcg ccagttcttg aggtcgattt
      541 ccttgtgagc gatattccag tcgccgcaga gaatgtattc gcgcccgctg ttccgaaggc
      601 cggagagatg cggcacgaac gcgtcgagaa aacggaattt tgacgcctgc cgcaattcgc
      661 ctgccgatcc tgacggcagg tacaccgaga cgatgctgag ttggcccaag cgaatctcga
      721 ggtagcggcc ctcgtcgtcg aactcgggta cgccgaagcc acgaacgatg gcgtcgggtt
      781 cgtgacgcga gtagatggca acgccgctat aaccttttcg aatagcgtcc tcgtagtagc
      841 aatggtaacc atccggggca aaacagggat cacccagctg atggtgttga gcttttgttt
      901 cctggatgca gacgacatcc ggcgtttgcg tcgagagcca gtcgaaaaag cctttgcgcg
      961 cggcggcgcg aataccgttc acattaacgg tgattacacg aaacaacttc atacctccgt
     1021 aatgaggtgc gccctgcaga tgtctgcatg aagcgacagc tgtgcaggac aggaattccg
     1081 gttgcaatgg gtgctactcg cgattcggaa aatccgaatg cggtatgtca tactcgccgg
     1141 cacgctgcgc gttgatcaac gggcaataat acagaagctg catccgagtc caacatgcgt
     1201 gcatacaaac aggaatttct cgatcttgca ctggagctgg gcgtactccg cttcggcgag
     1261 ttcacgctga aatccggacg tgtgagccct tacttcttca acaccggcct gttcaacacg
     1321 ggctatgcag cggcaaagct cgggcgctac tacgcggcgg caattgcagc atcgcgtgtc
     1381 gagtacgaca tgctcttcgg ccccgcgtac aaggggattc cactcgtcgc gctggcggcc
     1441 gcagctctcg ccgaacacca tgatgtggac gtgccgtaca ctttcaatcg caaggaagcg
     1501 aagaagcatg gcgagggcgg tgttgttgtg ggcgcgcctt tgagcggcaa agtcctgatc
     1561 gtcgatgacg tcatcactgc aggcacggcc gctcgcgagg cacagcagat catcaccggt
     1621 gcgggcgcgg aagtcgcggg cctggtgata tcgctggatc gtgaggaagt cggacaggat
     1681 tccagatcgg ctgtgcagga actggagcag acgcttaagg ttcctgtcat cagcatcgtt
     1741 cgcctcgagg acctcatcga tcttctcgag gaatcggacg aattcggcga gtacctggag
     1801 ccggtagtga ggtaccgcga cacctacggg atcaacgcgg ggctcgtaag cacctgactt
     1861 acgccttttt ggccgttcgc atccaactgc aagccactga cactcctgcg gaaaccgagg
     1921 tttacagcgg ctccacgcgc cactaagctg cattttcgcg aaccagtgcc tacttcgtcg
     1981 tctggcatgt gtgtgatagt cacccgatga gaaatttgct ccttacagcc gcatccctgc
     2041 tcctcctgtc acagggagct ctcggtgcgc aggaccagaa gctgtaccgc tgggtcgacc
     2101 aggaagggaa tgtccatttc ggcgacagca tcccggccga gtacgcagag gtcgaaaagc
     2161 aggtcgtaaa tgaacacggt gtcaccctcg acgtgctgag gggcaagaaa accgcggaag
     2221 aaatcgcggc ggaaaagaag gccgaggagc tgaaggcgga gcgcgagttg cgtcggcgcc
     2281 aggaccaggc actgctcgcc acttaccttt cggtggacga gatcgtgatg ca
//
LOCUS       DASUOM010000472         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_777664,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000472 DASUOM010000000
VERSION     DASUOM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777664"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2785)
                     /locus_tag="VFY27_09770"
     CDS             complement(<1..2785)
                     /locus_tag="VFY27_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5765844.1"
                     /translation="MPEQLRQGTFCRTKVSSAIRASLSLLALGVAAYHGPLLAQEEEP
                     ADQTSTDVADDDSRPIEEIITRGVRSSLESAQALKRNSSVIVDSVTAEDIGALPDRSV
                     TETLQRIPGVAINRFAAGRDPDHFSVEGSGVVVRGLTYVRSEVNGRDSFTANNGRGLS
                     FADVPPELLIGVDVFKSPSADRIEGGIAGTVNLRTRRPFDSQDPMFAFSLESNYGDFI
                     EKSSPTGSALGSWRWNTDAGEFGILGSVVYSQVRSRADRFQISNFAERTLYSNGDVVD
                     PMDGVTTPVDEVIFPRGAVMGSQEFDKERYGYSAALQWRSPDDDFEATFQFLRSDSRE
                     AWSERVVEIATDVVRDQGDSRAVPGTTFDFDSDNVFDRGTITGPSGWRDDQFSADPRT
                     PSFGLQSNNIRRDVKQKFVTDDFGMNFIWQATEDWAFSFDYQHVDSTVDNLDAGIWGS
                     TYQNAFLDLDGTDIPTVEFGPPILCSDTPSNTCATYMSAGHESFTDPYNSFYRAAMDH
                     VEESEGTSDAYRVDAERMLPGSGWLESVRFGYRFAERDQTARFSRYNWGSLSEIWGGG
                     GPVWFDDPIASNGGQPLMGYEAFHFENFFDGDTANPVGGNGRLFYAENIVDNYEAYAQ
                     YASQVAAEWPGGASWVPLADRAGTTGWFLPGEENPVKETNNAVYLMANFDTQLDNGWN
                     LTGNVGVRYTDTKREASGFQTFNAQIFTTDEVCDAPLPPGESPSPFCGLPADVRAEAR
                     AYSDGTIAPFTSTLDYDYWLPSFNVRLEVAEGVQFRAAYFRGVAPPDFGLTRAYYDIN
                     LETEADDIAAGNGRPIGRFNAGNPLLKPAESDNYDLTFEWYFSEVGQLSFALFHKEIS
                     NIRTNDVQRLSFTNNGATFDAIVTTAVNSREEGEIQGFEVAYQQTYDFLPGWLNGFGL
                     STNYTYVDSSNVPQ"
     gene            2957..>3630
                     /locus_tag="VFY27_09775"
     CDS             2957..>3630
                     /locus_tag="VFY27_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX5765845.1"
                     /translation="MPIAPEKPVRVLIVGGGTAGWMTAAALSRLLPPRLADVRLLESS
                     EIGTVGVGEATVPHIRFFNAKLGLDEADFMARTRATFKFGIEFRNWSRIGDSYIHPFG
                     AFGRDLAGVAFHHHFVRARQLRLARSIEPYSLPILAARRGKFAHPSEDFASALSTSSY
                     AYQFDAGLYAAYLRQYAEARRVRRTEGKIVDVRLNGESGFVESVLLESGEAIAADLFI
                     DCSGFRG"
BASE COUNT          663 a         1118 c         1115 g          734 t
ORIGIN      
        1 tctgcggcac gttggaggag tcgacgtagg tgtagttggt cgataatccg aagccgttga
       61 gccatcccgg caggaagtcg taggtctgct ggtacgcgac ctcgaagccc tggatctcgc
      121 cctcctcgcg cgagttcacg gcggtcgtca cgatcgcgtc gaaggttgcg ccgttgttcg
      181 taaacgagag gcgctgcacg tcgtttgtgc ggatattcga aatctccttg tggaacaacg
      241 cgaagctaag ctgcccgact tcggagaaat accattcgaa cgtcagatcg taattgtccg
      301 actctgccgg tttcagtagc gggttgccgg cattgaaacg gccgatcggc cgcccgttgc
      361 cggcggcgat gtcgtcggct tcggtttcca ggttgatgtc gtagtatgcg cgcgtcaggc
      421 cgaaatccgg aggcgcgaca cccctgaagt atgccgcccg gaactgcacg ccttcggcca
      481 cttcgagtct cacgttgaag ctcggcagcc agtaatcgta atccagcgtg ctggtaaatg
      541 gcgcgatcgt gccgtccgag taagcacgcg cctcggcgcg gacatccgca ggaagcccgc
      601 agaacgggct cggcgattcg cccgggggca gcggtgcgtc gcacacctca tcagtcgtga
      661 atatctgcgc gttgaacgtc tggaacccgc tcgcctcgcg tttcgtgtcg gtgtaccgga
      721 cgccgacgtt accggtcagg ttccaaccgt tgtccagctg ggtgtcgaaa ttcgccatca
      781 ggtaaaccgc gttattggtt tcctttaccg ggttctcttc gcccggcagg aaccagcccg
      841 tcgtgcccgc gcggtcagcc agcggcaccc acgaggcgcc acccggccat tcggcggcga
      901 cctgcgaggc gtattgggca taagcttcat aattatcgac gatgttttcg gcatagaaga
      961 ggcggccgtt gccgcccacc ggattggcag tgtcgccgtc gaagaagttt tcgaagtgga
     1021 aagcttcata gcccatgagc ggctggccgc cgttcgatgc aatgggatcg tcgaaccaga
     1081 ccgggccgcc gccgccccag atttcgctga gactgcccca gttatagcgc gagaagcgcg
     1141 cggtctggtc ccgctccgcg aagcggtaac cgaagcgtac ggactcgagc cagccgctcc
     1201 cggggagcat gcgctccgca tctacacggt acgcgtcgga cgttccctcg ctctcctcga
     1261 cgtgatccat cgcggcgcgg tagaaggagt tgtacgggtc ggtaaagctt tcgtgaccgg
     1321 cgctcatgta tgtcgcgcag gtgttggaag gagtatccga gcacaggatc ggcgggccga
     1381 actcgactgt cgggatgtcc gtcccatcca ggtcgagaaa agcgttctgg taggtcgaac
     1441 cccatatgcc ggcatccagg ttgtcgacgg tggaatccac atgctggtaa tcgaagctga
     1501 acgcccagtc ttcggtggct tgccagataa aattcatgcc gaagtcatcg gtgacgaact
     1561 tctgcttcac gtcgcgacgg atattgttac tttgcaggcc gaacgatggc gtacgcgggt
     1621 cggcgctgaa ctggtcgtcg cgccagcccg aagggccggt gatcgtgccc cggtcgaaca
     1681 cgttatccga atcaaagtcg aaggtggtgc cgggcacagc gcgcgagtcg ccctggtccc
     1741 tgaccacgtc cgtcgcaatt tctacaactc tctcggacca tgcttcgcgt gaatccgaac
     1801 gcaggaactg gaacgtcgct tcgaaatcgt cgtcggggct gcgccactgc agagcggcgg
     1861 agtagccgta acgttccttg tcgaattcct gcgagcccat gacggcgccg cgcgggaaga
     1921 tgacctcatc gaccggcgtc gtcaccccgt ccatcgggtc aacgacgtcg ccgttcgaat
     1981 acagcgtgcg ttccgcgaag ttggatatct ggaagcggtc cgctcgcgag cgcacctgcg
     2041 agtaaacgac gctacccagg attccgaact cgccggcgtc agtgttccag cgccagctac
     2101 cgagcgccga gcccgtgggg gaggatttct cgatgaaatc gccgtaattg ctttccagcg
     2161 agaatgcgaa catcgggtcc tggctgtcga acggcctgcg cgtgcgcagg ttgacggtac
     2221 cggcgatgcc tccctctatg cggtcggcag acggactctt gaatacgtcc acgccgatca
     2281 ggagctcggg cggcacatcg gcgaagctca accccctgcc gttgtttgcc gtaaacgaat
     2341 cgcgcccatt cacctcgctg cgaacgtaag tgaggccgcg aaccactacg ccgctgcctt
     2401 ctaccgagaa gtgatccgga tcgcggccgg cggcgaaacg gttgatggcg acgcccggga
     2461 tgcgctgcag tgtctcggtg acggagcggt ctggcagtgc gccgatgtcc tcggcggtaa
     2521 cggagtcgac gattaccgat gagtttcttt tgagcgcctg cgcggattca aggctgcttc
     2581 ggacgccgcg agtgatgatt tcctcgatcg gtctcgagtc gtcatccgca acgtcagtgc
     2641 ttgtctggtc ggcgggttcc tcctcctggg cgagtagcgg gccgtggtag gcggcgaccc
     2701 ccagggccag gagcgacagg cttgcgcgaa tcgcggatga gaccttcgtg cgacaaaagg
     2761 taccctgacg taattgctcc ggcatgtctc ccccttcaca gccaaaacgt tagcgttagc
     2821 gctaacatct tgtataatct tcttcgacaa taaagtcaac aaatagccga tttttcaacg
     2881 ttcagggccg agttggttat attgccgggg tcgcacctgt cccgatcccg cccgacactg
     2941 cgagggggaa agcagtttgc caatcgctcc ggaaaaaccc gtacgtgtcc tgatcgtcgg
     3001 cggcggcacc gccggctgga tgacggccgc ggcgctctcc cggttgctgc ctccgcgcct
     3061 ggcggatgtc cggctgctgg aatcctcaga aatcggcacc gtcggtgtcg gcgaagccac
     3121 cgtcccgcac atccgttttt ttaacgcaaa gctcgggctg gacgaggccg atttcatggc
     3181 ccgtacccgc gcgaccttca agttcggcat cgaattccgc aactggtcac gcatcggcga
     3241 cagctatatt catccattcg gggcttttgg ccgggacctt gccggtgtcg ccttccatca
     3301 ccacttcgtg cgagcgcggc aattaaggct cgcaaggtcg atcgagccgt attcgctgcc
     3361 gatactcgca gcgcggcgcg gcaaatttgc ccatccttcc gaagatttcg cctcggcact
     3421 ctccacctcc tcctatgcgt accagttcga cgccggtctc tacgccgcct accttcggca
     3481 atacgccgaa gcccgccgcg tgaggcggac cgaagggaaa atcgtggacg tcaggctcaa
     3541 cggtgaaagc ggctttgtcg agtcggtcct tctcgagagc ggtgaggcca tcgccgccga
     3601 cctgttcatc gactgctcag gcttccgcgg
//
LOCUS       DASUOM010000473         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_55502,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000473 DASUOM010000000
VERSION     DASUOM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55502"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFY27_09780"
     CDS             <1..815
                     /locus_tag="VFY27_09780"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5765846.1"
                     /translation="RPNTCQGCLNLAAIVLIAFFAGVSGCAAQTEDPAPAQTIPVPDA
                     EGTAIENDQAGENTITIDPNVVSYDEYHDPLMPLNRFVFRFNDVLARYALVPLGKGYA
                     RVVPEAVDHRVDNFFSNVALPIYAVNNLLQAEPRLSGRNLARFGINTTVGLLGLFDPA
                     EAWFGLEPAEADFATTLAHHDVGYGVYLVLPFFGPSDGRNAAARVVDYFLHPIPWVLD
                     QPEAFVLMSYGLFHEFAQDAEDYERLLEQSEDPYIFMRNLHLQSVQRDAAYR"
     gene            828..>2021
                     /locus_tag="VFY27_09785"
     CDS             828..>2021
                     /locus_tag="VFY27_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5765847.1"
                     /translation="MRRLNVTGAWTVLLLAAVITAALGWQARKFEIDASADTLLTRDN
                     EKYIQTKLAGQEFSPEEFLFVAYQPKDRPLLSQETFDELEAIAGEIGELERVASVRSI
                     LNVPLPTLGDTGDLTSFDPSTLTIERAGFSTDAVSADLRDHPIYEDLLIDAEQKVTAL
                     QVLFRPHARLDELHGRITALEAIGLNRTLQEDEEAELERLRLSADPLEKELTETRNQE
                     VQAIRGIASKYEDNANIYLGGVHVLGYQLVQIIRNDLVVFGAAIAALVALLLLVLFRE
                     LRAVVIAFACCLVSITCTIGLFGLLGLKATVISANFVALQLILTLALVVHLMVQYREY
                     ARANAEWDQARLVRETMRTKAPPSVYAGITTGIGFASLLASGIQPVISFGWMMVIAIA
                     ISLAVT"
BASE COUNT          407 a          614 c          603 g          397 t
ORIGIN      
        1 accgaccgaa tacttgccag gggtgcttaa accttgccgc aatagtcctt attgccttct
       61 tcgcaggcgt tagcggctgt gctgcgcaga cggaggaccc ggcgccagcg caaacaatcc
      121 cggttccgga tgccgaaggc acggcgatcg agaatgacca ggcgggcgag aacaccataa
      181 cgatagaccc caatgtcgtc agctacgacg aataccacga ccccctgatg ccgctgaacc
      241 gcttcgtctt ccgcttcaat gacgtcctgg cacgctatgc cctcgttccg ctcggaaagg
      301 gctacgcgag ggtcgtaccg gaagcggtcg accaccgcgt cgataatttc ttctccaatg
      361 ttgccttacc catatacgcc gtgaacaacc tgctgcaggc ggagccccgc ttgagcgggc
      421 gcaaccttgc gcgtttcggc atcaacacaa ccgttgggct gttgggactt ttcgatccgg
      481 cagaagcgtg gttcgggtta gagccggccg aagcggattt cgccaccacg ctggcgcacc
      541 acgatgtcgg ctacggcgtc tacctggtgt tgcctttttt cgggccatcc gatggacgta
      601 atgcggcggc gcgggttgtg gactacttcc ttcacccgat tccatgggta ctcgaccagc
      661 ccgaggcgtt cgtcctgatg agctacggct tgttccacga gttcgcgcaa gacgccgagg
      721 actacgagcg attgctggag caaagcgaag acccttacat tttcatgcgc aacctgcacc
      781 tgcagagtgt ccagcgcgat gccgcatacc ggtagcagac gccgcgcgtg cgaaggctga
      841 acgtcaccgg agcatggacg gtcctgctgc tggctgcggt aatcacggca gcattggggt
      901 ggcaggcaag aaagttcgag attgacgcat cggccgacac cctgctgacg cgcgacaacg
      961 aaaagtacat tcaaaccaag ctggcaggcc aggagttttc gccggaagaa ttcctgtttg
     1021 tcgcgtacca gccgaaagac cggccactgc tatcgcagga aacgttcgac gaactggagg
     1081 ctatcgccgg tgagattggc gagctcgagc gggtagcatc ggtgcgctct atcctgaacg
     1141 tgccactgcc gacacttggg gatacaggag atctcacgag cttcgatcct tccactttga
     1201 cgatcgaacg cgccggcttt tccaccgacg ccgtttccgc ggacttgcgc gaccatccaa
     1261 tctacgagga cctgctgatc gatgcggaac agaaggtcac cgcgctgcag gtgctgtttc
     1321 gtccccacgc caggcttgat gaactgcatg gcaggataac ggctctggag gcaatcggcc
     1381 tgaaccggac gctgcaagag gacgaggaag ctgaactcga gcggttacgg ctttcggccg
     1441 acccgttgga aaaggaactg accgagactc gcaaccagga ggtccaggcc atccgtggta
     1501 tcgcgagcaa gtacgaagac aacgcgaaca tctacctcgg cggcgtacat gtgctcggtt
     1561 accagctcgt gcagatcatc cgcaacgacc tggtcgtctt cggcgcggcc atcgccgccc
     1621 ttgtcgccct cctcctgctc gtgctgtttc gtgagctgcg cgcggttgtc atcgcatttg
     1681 catgttgcct ggtgagcatc acctgcacga tagggctctt cggcctgctc gggctcaaag
     1741 ccacggtgat atcggcaaat ttcgttgccc tgcagctcat actgaccctc gcgctggtcg
     1801 tgcacctcat ggtgcagtac cgcgagtacg cgcgggcgaa cgcggagtgg gaccaggcaa
     1861 ggctggttcg cgaaaccatg cgcaccaagg cgccgccgag cgtgtacgcg gggattacga
     1921 caggcatcgg cttcgcttca ttgctggcaa gcggcataca gccggtcatc agcttcggct
     1981 ggatgatggt catcgcaata gccatatccc tggcagtgac g
//
LOCUS       DASUOM010000474         8650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_470146,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000474 DASUOM010000000
VERSION     DASUOM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470146"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VFY27_09790"
     CDS             <1..2066
                     /locus_tag="VFY27_09790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5765848.1"
                     /translation="SESSDLYQKLVLEDQSVDQLVAYFPNRKDPNLLMIYARLTDEKH
                     AADVESAINGTLAEARTEMVTRKRLEETKSRLRYLFTSQLDSAGGIGSVLAAFVQFDR
                     TPETINEVYQGYDALTPEDIRDAANRYFVDSGRVTVSLSSNESMAGIGGKSSIDALVE
                     TLAADAGSASDAAPANEPEAETEQEPVRDAAATPVAQAGQPEEASPVSVVAQPSASSP
                     LVDVSFVVHAGAAMDPDGKKGLASLTAAMIAEGGSTSHTIQQINDAMYPIAASFDAQV
                     DKEMTRLAGQVHKDNLDTWYGLISGQLLSPGWREQDFRRVKTQLINSIRTSLVANNDE
                     ELGKEVLYSEIYGAGHPYGSLNLGHVRDIEAITLADVQKFYADYYTVRNIRLGLGGGY
                     GDDFASRVASDLQRLPAGSRARPEVPPAPGVETNRAVIVEKETPAVAVSLGFPIELTR
                     GDPDWLALWLARSYLGEHRNSNARLYQRIREERGMNYGDYAYIEYFPRGMFQFHPDTN
                     LARQQQIFQIWIRPLRNNNDAHFATRSAVYELQKLIDEGMSESDFEATRSFLSKFVSL
                     LTDGQSRQLGYEIDSRYYEIDRFSDYVRDGLASLTLDDVNRVIRENLDTEKMQYVFVA
                     KDANDLRERLVEDSPSPISYDSEKPAELLEEDREIAALPLNFEPEMVRILPASEVFDG
                     NSRGS"
     gene            complement(2252..4051)
                     /locus_tag="VFY27_09795"
     CDS             complement(2252..4051)
                     /locus_tag="VFY27_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5765849.1"
                     /translation="MDQAQIIFLIILAAALVLLVTEWVRLDLTGVFIIIALAASGLLT
                     SAEALSGFSSEPAILVASMFVLSGGLSQTGLTERLGLWIGQLSGDSIWRAVLVIMPSV
                     ALMAAFSHHLMVTAMMLPVVLNLNRSRNLPASRLMIPMAFAASLGTTITVIAAPSFLV
                     ARDLLRRSGGHDIGIFDITPVGIVLCLVGLAYVLLLGRWLLPTHEGAAAEDDRFRLER
                     YYTELVVLEDSPHSGKTMQEFREQHKDRFQVVDWLRHGHSRGHPWSRKELAAGDLLLV
                     RASPDELATLEKQQGLALHAVVQYGEVIPDEKRKELRGSERLVQAVVAPDSELVGRNI
                     GSVDFLSRYGVVVVGLWRKRGFMRKELSKVQLRAGDLLVLWGEQKALDQLAGNRSFLL
                     LVPFSARERTLGKGGLAASIMAASVIAAATGLLPVTIAFLAGAAAMVASGCLSLNQAY
                     ESVEIRIFVFIAGAIPLGLAMEQTGMATLLAEWLSQAATSWQTMWVLLALFISAAVLT
                     QVLSDTATTVLLAPVALGMAPLLGISAPATVFSVAMGAVVAFLTPIGHHGNLLIYEPG
                     GYRFTDFVRAGTPLTLLLGFAVAFLAPIVWPPA"
     gene            complement(4543..6894)
                     /locus_tag="VFY27_09800"
     CDS             complement(4543..6894)
                     /locus_tag="VFY27_09800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5765850.1"
                     /translation="MGKYGTGQAIRRVEDQRFITGTGRYTDDISFDGQTSLYFFRSPY
                     AHGIVRSLDVTAARAAPGVIAVYTSEDLVAAGVRDVPGAALPASAIGGAKPALQQPPL
                     ARDRVRYVGEPVVAIQANSLPEAKDASELIELEVEELAAAVNPADAVLDTAQTIQELA
                     PGNVYGHMTYGDSNRADAAFDGAAHVVSIDLVNNRLAPTAMEPRGCNVTFDPSNEKIT
                     VHQGCQGAHSLRERILETVDMDPEKLQVISPDVGGAFGLKFFLQCETVVAVLACRQSR
                     VPVKWIADRSESFLSDLHGRDHVTHAELALDADGRFLAVRAAITGNIGAYCSQAGPII
                     PWFGACMTTGVYAIPAAWVDVRTVVTNTVPVDAYRGAGRPEAAYLIERLVDKAARQIG
                     IERTELRRRNLIQPAQFPYRTATGRTYDSGEYEAVMDAALTRASFEHFEQRRAKSLEA
                     GRLRGIGLACYVEICSIMGGENAYIEFTTQGRVSVRTGTQSTGQGHETSYGQMVAARL
                     GVGIDSIDFIQGDTDRVPTGEGTAGSRSMAIGGSAICLTTDRVIDTGRRMAAELLEAA
                     DADVEFEQGTFRIAGTDRGLSLTDVALASFDDGKRPDDVQPGLASSERFEPAEGTFPN
                     GCHVCEVEVDPETGTIEILRYTIEDDVGNVINPLILEGQIVGGVAQGLGQALGEHAVY
                     DPDGGQLVTASFMDYPMPRADWIPVIDFRYREVPSPRNPLGVKGAGEAGTVGAAPALV
                     NAVLDALAPRGVEHLDMPLTPLKIWTALNAQIRESVQTHEETP"
     gene            7182..7439
                     /locus_tag="VFY27_09805"
     CDS             7182..7439
                     /locus_tag="VFY27_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX5765851.1"
                     /translation="MTPANRTAYWRTNLRLVAACMAVWFTCSYVFGILLVEQLNRITI
                     GGFKLGFWFAQQGSIYIFVLLIFFYAWRMNRIDEQHDVHED"
     gene            7447..>8650
                     /locus_tag="VFY27_09810"
     CDS             7447..>8650
                     /locus_tag="VFY27_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX5765852.1"
                     /translation="MELKTLTYLVVGATFALYVGIAVWSRARNTGDFYIAGKGVSPVA
                     NGMATAADWMSAASFISMAGIIAFSGYDGSVYLMGWTGGYVLLALFLAPYLRKFGKFT
                     VPEFIGERYYSKTARIVAVICLIFISFTYVAGQMRGVGIVFSRFLEVEVTVGLMVGMG
                     VVFVYAVLGGMKGITYTQVAQYCVLIFAYTVPAVFIAIQITGNPVPQLAFGSDIAGTG
                     TSLLEKLDDIVTSLGFNEYTSGTKSKIDVFCITLALMVGTAGLPHVIVRFFTVPKVRD
                     ARISAGYALLFIAVLYTTAPAVGAMARYNLANTILPGEAGEATGNIRYEDRPGWMKTW
                     EQTGLITFEDRNGDGRIQYYNDANPEFAETAQRYGWRGSELTVDNDIVVLANPEISRL
                     PNWIIALVA"
BASE COUNT         1654 a         2728 c         2670 g         1598 t
ORIGIN      
        1 tctccgagtc ttccgacctg taccagaagc tggtgctgga ggaccagtcg gtagaccagc
       61 tcgtggcgta tttcccgaac cggaaggacc cgaacctgct gatgatctac gcgcgcctga
      121 cggacgagaa gcacgcggcg gacgtcgagt ccgcgatcaa cggaacgctc gccgaggcgc
      181 ggacggaaat ggttacccgg aagcggctgg aggagaccaa gtcgaggctc cgctacctct
      241 ttacctcgca actcgacagc gccggcggga tcggcagcgt cctggccgca ttcgtgcagt
      301 tcgatcgcac gccggagacg atcaacgagg tttaccaggg ctacgacgca ctcacgcccg
      361 aagacatccg ggatgcggcg aaccgttact ttgtcgactc cggccgggtc acggtaagct
      421 tgtcgagcaa tgaaagtatg gccggcatcg gcggcaaatc atcgatcgat gccctcgtag
      481 agaccctggc ggccgatgcc ggcagcgcaa gtgatgcggc gcccgccaat gaacccgaag
      541 ccgaaactga gcaggaaccg gtgcgggatg cagctgccac tccggtcgcg caggcagggc
      601 agccggagga agcgtcgccc gtgtccgttg ttgcacagcc ttccgcgtcc tcgccgttgg
      661 tcgacgtctc cttcgtggtg catgcaggcg ctgcgatgga tcccgacggc aagaagggac
      721 ttgcatcact gaccgcggcc atgatcgcgg aaggcggctc gacctcccac acgatccagc
      781 aaatcaacga cgcgatgtac ccgatcgcgg cgagcttcga tgcccaggtc gacaaggaga
      841 tgacccggct cgcgggacag gttcacaagg acaacctgga cacttggtac gggctgatca
      901 gcggccagct gctgagcccc ggctggcgcg agcaggactt caggcgcgtg aagacgcagc
      961 tcatcaactc catccgcacc agcctcgttg ccaacaacga cgaggaactc ggcaaggagg
     1021 tgctgtacag cgagatctac ggcgccgggc acccgtacgg gtcgctcaac ctcggtcacg
     1081 tgcgtgacat cgaggctatc acgctggcgg acgtacagaa attctacgcg gactattaca
     1141 ccgtccggaa catcaggctg ggtctcggcg gcgggtatgg cgatgatttt gcgtcgcggg
     1201 tcgccagcga cctgcagcgc cttccggccg gcagccgggc gcgcccggaa gttccaccgg
     1261 cgcccggggt ggaaacgaac cgagccgtga tcgtcgagaa ggaaacgccg gccgtcgccg
     1321 tttccctcgg cttcccgatc gaactgacgc gcggcgatcc ggattggctc gcgctgtggc
     1381 tcgcccgctc gtacctcggt gagcatcgca acagcaacgc gcgtctttac cagcgaatcc
     1441 gggaagaacg cggcatgaac tacggggatt acgcgtacat cgaatacttt ccgcgcggca
     1501 tgttccagtt tcatcccgac accaacctgg ctcggcaaca gcagatcttc cagatctgga
     1561 tacggccgtt acgcaacaac aacgacgccc atttcgccac gcgcagcgcg gtatacgaat
     1621 tgcaaaagct catcgacgag ggcatgtccg aatcggactt cgaggcgacg cgaagcttcc
     1681 tctcgaagtt cgtcagcctg ctgacggacg ggcaatcgcg ccagctcggc tacgaaatcg
     1741 acagccgcta ctacgaaatc gatcgtttct ccgactacgt gcgcgacggc ctcgccagcc
     1801 tcacgctcga cgatgtcaat cgcgtgatcc gcgaaaacct cgacacggag aaaatgcagt
     1861 acgtcttcgt ggcgaaagac gcgaacgacc tgcgcgaacg cctggtcgaa gattcgccat
     1921 cgccgatctc ttacgactcc gagaagccgg cggaactcct ggaggaagac agggaaatcg
     1981 cagcgctgcc actcaacttc gaacctgaga tggtacgcat actcccggca tccgaggtct
     2041 tcgacggcaa tagccggggc tcgtagctcg catactcagg aacgaatttc acgatcatcg
     2101 ttaaggcatg gccggcgggg cggcctttgg cggaatggct cccttcggtc cctttatttc
     2161 cgccaaaggc taccccaccg accatgcctc ccgtcgtttt cgcaccctcc gttatcctta
     2221 gcttcagcgc cagtgaacga actgatcgcg ctcaggcggg tggccacacg atcggtgcga
     2281 ggaaggctac ggcgaagccg agcaacagcg tcaacggagt gcccgcgcgt acgaagtcgg
     2341 tgaagcggta accgcccggt tcgtagatca gcaggtttcc gtgatggcct atcggcgtga
     2401 ggaatgcgac gacggctccc atcgccacgc tgaagaccgt cgcgggcgcg gagatgccga
     2461 gcagcggcgc catgccgagc gctacaggtg cgagcagcac cgtcgtcgcc gtatccgaga
     2521 gtacctgggt cagcacggca gcgcttatga agagcgccag cagcacccac atcgtctgcc
     2581 atgaagttgc cgcctgcgag agccactcgg cgagcagcgt cgccatgccg gtctgctcca
     2641 tcgcgaggcc gagcgggatc gcgccggcta tgaatacgaa gatgcggatc tccaccgact
     2701 cgtacgcctg gttcagcgag aggcatcccg acgcgaccat tgcggcggcg ccggccagga
     2761 atgcgatcgt caccggaaga aggccggtcg ctgcggcaat gacgctggct gccatgatcg
     2821 atgcagcgag cccgcctttc ccgagcgtcc gttcgcgcgc ggagaagggc accagcaaga
     2881 ggaaggaacg gttgcctgcc agctggtcga gcgccttctg ttccccccac agcaccagca
     2941 ggtcgcctgc gcgaagctgc actttggaga gttccttgcg catgaatccg cgtttgcgcc
     3001 ataagcccac gacgacgacg ccgtagcgcg agaggaaatc cacgctgccg atgttgcgcc
     3061 ccaccagttc ggagtccggc gcgaccaccg cctgtacgag tcgttcgctg cctctcagct
     3121 ccttgcgctt ctcgtccggg attacctcgc cgtactgcac tactgcgtgg agcgcgagtc
     3181 cctgctgttt ctcgagcgta gcgagctcat ccgggctggc gcggacgagc agcaggtcgc
     3241 cggctgcgag ctccttgcgg ctccaggggt gcccgcgtga atggccatgc cgcagccagt
     3301 cgactacctg gaaacggtcc ttatgctgct cgcgaaactc ctgcatcgtc ttcccgctgt
     3361 ggggcgagtc ctcgaggacg accagctcgg tgtaatagcg ctccagccgg aaacggtcat
     3421 cctctgcggc ggctccttcg tgcgtcggca gcagccagcg gccgaggagg aggacgtagg
     3481 cgagaccgac caggcacagc acgatgccta caggcgtaat gtcgaaaatg ccgatgtcgt
     3541 gcccgcccga gcgcctgagc aggtcgcgcg ccaccaggaa cgacggggcc gcgatcaccg
     3601 tgatggtggt gcccaaggat gcagcgaacg ccatcgggat catcagccgg gatgcaggga
     3661 gattcctgct gcgattcagg ttcaggacca cgggaagcat cattgccgtc accatcaggt
     3721 gatggctgaa cgccgccatg agcgcgacgg acggcatgat caccagcacc gcccgccaga
     3781 tgctgtcgcc ggaaagctgg ccgatccaca ggcccagccg ttcggtgagt cctgtctggc
     3841 tcagtccgcc ggacaggacg aacatggatg cgaccaggat cgcgggctca ctggagaaac
     3901 cggagagtgc ttctgcagac gtcagcagcc cgcttgcggc cagcgcaatg atgatgaaga
     3961 cgccggtgag atcgagccgt acccactccg tgaccagcag cacgagcgcc gccgcgagga
     4021 tgatcaggaa aatgatctgg gcctggtcca tggggaatcc gtcgacggag cggacatgtt
     4081 agtgggtcgg ggtgcggaag agaaagcgag gcgggggccg gtgcccccgc cgtgcctgtt
     4141 ggtaaagaac cacaaccggt cgtgaagcaa aatgtgtgcc cgatgcccgg ctataaagaa
     4201 ttgacattga gccggaattc tgcgaagttc tggacccatg cctcgtagct tttgcacaac
     4261 agcgttgttg caagaagccg caagatcgtt tcgacaacac cttcaggtac gacttggcgg
     4321 ggggatttta agtcaaggct cttgtactga tgatggtaag gcggctcctt cgggagccgc
     4381 ttttttgtgg tgcgcccggc gaggcgacgt ggcttaggga tgccaatgta ggagcccgcc
     4441 atgcgggcga gttcgctgag cgcagatcgc actcgcccgc attcgccgtc cgccaagcga
     4501 cgcggattcg gtaaggccgc aggcttcgct cgacgggggc tcctacggtg tttcttcgtg
     4561 cgtctgcaca gactccctga tctgcgcatt cagggcggtc cagattttca aaggcgtgag
     4621 cggcatgtcg agatgctcga cgccgcgcgg cgccagcgca tcgagaacgg cgttgaccag
     4681 cgcaggtgcc gcgcccactg ttcccgcctc gccggctcct ttcaccccca gcggatttct
     4741 gggtgacggc acctcgcgat agcggaagtc gatcaccggg atccagtccg cgcggggcat
     4801 cgggtagtcc atgaaggatg ccgtcaccag ttgcccgccg tcggggtcgt aaaccgcgtg
     4861 ctcaccgagt gcctggccca gtccctgggc gacaccgccc acgatctgtc cttccaggat
     4921 gagcgggttg atgacgttgc cgacatcgtc ttcgatcgtg tagcgcaata tttctatcgt
     4981 gccggtttca ggatcgactt ccacctcgca gacatggcaa ccattgggaa aggtaccctc
     5041 cgcgggttcg aagcgctcgc tgctggcaag tcccggctgc acgtcatccg gtctcttgcc
     5101 gtcatcgaag gaggcgagag ccacgtcggt gagcgacagg ccgcggtccg tgcccgcgat
     5161 gcggaacgta ccctgttcga actcgacgtc cgcgtccgcc gcctcgagca attcggccgc
     5221 catgcgccgg ccggtgtcga tgacgcgatc ggtcgtcagg cagatggccg aaccgccgat
     5281 cgccatcgag cgggagccgg cggtgccttc tcccgtcggc accctgtccg tgtcaccctg
     5341 gatgaagtcg atgctgtcga tacccacacc cagtcttgcg gcgaccatct ggccatagct
     5401 ggtctcgtga ccctgcccgg ttgactgggt gccagtgcgc acggaaaccc tgccctgggt
     5461 cgtgaactcg atatacgcgt tctcgccacc catgatcgag caaatttcga cgtagcaggc
     5521 gaggccgatc ccgcgcagcc gtcccgcttc caggctcttc gcgcggcgct gctcgaaatg
     5581 ctcgaacgat gcgcgcgtca acgcggcgtc catgacggcc tcgtattcgc ctgagtcgta
     5641 agtgcgcccg gttgccgtgc ggtaagggaa ttgcgccggc tggatgagat tgcggcgcct
     5701 gagctctgtg cgttcgatgc cgatttgccg cgccgccttg tccacgagtc gctcgatcag
     5761 gtatgcagct tccggtcgcc ccgcgccgcg gtacgcatcg accgggaccg tgttcgtgac
     5821 gaccgtgcgg acgtcgaccc acgcagccgg tatcgcatag acacctgtcg tcatgcaggc
     5881 gccgaaccac gggatgatgg gtcccgcctg cgagcaatat gcgccgatgt tgcctgtaat
     5941 cgccgcgcga accgcgagaa atcgcccatc ggcatccagc gcgagttccg catgagtgac
     6001 atgatcccgc ccatgcaggt cggataaaaa gctctccgaa cgatcggcga tccacttgac
     6061 gggcacacgg ctttgcctgc aggccaacac cgcgacgacc gtttcgcact gcaggaagaa
     6121 cttgaggcca aaggcacccc cgacgtcggg cgagattacc tgcagctttt ccgggtccat
     6181 gtcgaccgtt tccagtatgc gctctcgcag agagtgcgca ccctggcagc cctggtgcac
     6241 ggtgatcttt tcgttagacg ggtcgaacgt aacattgcat cctctcggct ccatcgccgt
     6301 aggcgcaagc ctgttgttga cgaggtcgat cgacacgacg tgcgctgcgc cgtcgaaggc
     6361 cgcatccgca cggttactgt caccataggt catgtgccca tacacgtttc ccggcgcaag
     6421 ctcctgaatc gtctgtgcgg tatccagcac tgcatccgcg ggattcacgg ctgccgcaag
     6481 ctcctccacc tccagttcga tcagttccga cgcgtccttc gcttcgggaa gcgagttcgc
     6541 ctggatcgct acgacaggct ccccgacgta tctcacgcgg tcgcgcgcca aaggtggctg
     6601 ctgcagcgcc ggcttcgcgc cgccgatggc acttgccggc agtgcagcac cgggaacgtc
     6661 gcgcacaccg gcagcaacga gatcttcgga cgtataaact gcgatgacgc caggcgcggc
     6721 acgagcggcc gtgacatcga gcgaacggac gatcccgtgc gcgtaaggcg agcggaagaa
     6781 gtaaagcgag gtctgcccgt caaaagagat gtcgtccgta taacggccgg tgccggtaat
     6841 gaacctctgg tcttctaccc ggcggatggc ctggccggtg ccgtacttgc ccacttggtg
     6901 cttctctccc tgtagagaac ctgcccacat tctgtggatg cggttggtag gagcccgcca
     6961 tgcgggcgag tctggtgatg ttatcgcact cgcccgcgtt cgccgcccgc aagcgtaaat
     7021 taaatgcgat atcgttcctg attccttcta acgtgggctc ctacggcgct tgacaatgcg
     7081 attcgaaact accacagcaa tgaatatttt tgccggctct gctagattgt ccgctgcctc
     7141 acggcgccac caaacaagat ctgcagaaaa taaaagccac gatgactccc gcgaaccgta
     7201 ccgcctactg gcgcaccaac ctgcgactcg tcgccgcctg catggctgtc tggttcacct
     7261 gctcctatgt cttcggcatc ctcctcgtgg aacaactaaa tcgcatcacg attggtggtt
     7321 tcaagctcgg gttctggttc gcacaacagg gttcgatcta tatcttcgtg ctcctcatat
     7381 tcttctatgc ctggcgcatg aacaggattg acgaacagca cgacgtgcac gaagactgat
     7441 tccatcatgg agctgaagac cctgacgtac ctggtggttg gcgcgacatt tgcgctgtac
     7501 gtcgggatag cggtctggtc ccgcgcccgt aataccggcg acttctatat tgcgggcaag
     7561 ggcgtatcgc cggttgcgaa cgggatggcc actgccgccg actggatgag cgccgcctcg
     7621 ttcatttcga tggccggcat catcgcgttt tcggggtacg acggctcggt ttacctgatg
     7681 ggctggacag ggggctacgt gcttctcgcg ctgttcctcg cgccttacct gaggaagttc
     7741 ggcaagttca cggtgccgga attcatcggg gagcgctatt actcgaagac ggcgcgcatc
     7801 gtcgcggtca tctgcctgat cttcatctcg ttcacgtacg ttgccggcca gatgcgcggg
     7861 gttggaattg ttttctcgcg cttcctggaa gtggaggtca ccgtcggcct gatggtcggc
     7921 atgggagtcg tcttcgtcta tgccgtgctc ggcgggatga aaggcatcac ctacacccag
     7981 gtggcgcagt actgcgtgct gatctttgcc tacaccgtgc ccgcagtgtt catcgcaatc
     8041 cagatcaccg gcaatcccgt tccccagttg gccttcggca gcgatatcgc cggtacgggt
     8101 acgagcttgc tggaaaagct ggacgacatc gttacctcgc tcggcttcaa cgagtatacg
     8161 agcgggacca agagcaagat cgacgtgttt tgcatcacgc tcgcgctgat ggtcggaacc
     8221 gcgggccttc cgcatgtcat cgtgcgcttc ttcacggtgc caaaggtgcg ggacgcgcgt
     8281 atctcggcag gctacgcgct gctgttcatt gccgtcctgt acacgactgc acccgccgtc
     8341 ggcgccatgg cccgctacaa cctggcgaac accatccttc cgggcgaagc tggcgaggca
     8401 accgggaaca tccggtacga ggataggccc ggctggatga aaacctggga acagacgggg
     8461 ttgataacct tcgaagacag gaacggcgac ggccgtatcc agtattacaa cgacgccaat
     8521 ccggaattcg cggaaacggc acagcgttac gggtggcgtg gcagtgaact gaccgtcgac
     8581 aacgatatcg tggtgctcgc caaccccgaa atctcgcggc taccgaactg gatcatcgca
     8641 ctggtcgctg
//
LOCUS       DASUOM010000475         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_553527,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000475 DASUOM010000000
VERSION     DASUOM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553527"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1699)
                     /locus_tag="VFY27_09815"
     CDS             complement(140..1699)
                     /locus_tag="VFY27_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765853.1"
                     /translation="MSASPAGTAHAAMSMDESGQALDAPIDLLPEALGPYSWPISTES
                     GQAQAYFDQGMQLRYAFNVDEAARTMLAAQRLDPQCAMCYWGEAFALGSYLNGAMTVE
                     KSQRAYAAIVRAAELAPSNTSEVERDLIAAASVRYPEDYDPANRRAVDTAFAEAMAQV
                     YEKYPDDHNVATVYAVALFMLEERRGYRDIDDPDVVRLHGVLTGVLEEDPRHPGACHL
                     YIHATESTRKPELALPCAEYLSSAVPVASHMQHMPSHTWNEVGLWGKSVEANTLAWHS
                     DLKAGEGRGFSYGPSHNLHMLLYAASFDGQGAVATQAGKDYRKITGNSMYEVLTLLRF
                     GRFDEILENERRPADEVGAALWDFARGYASLRQGDLRTAKAMREKVLEFSSTSDAMFR
                     FHPAERLAGAVARILEGEILLDAGDSEAAISAFEKAVALEDALGYDEPEPLPFAARHW
                     LGAALLEAGRHSDAERVYREELADHPNNGWSLYGLKLALEMQDRQDADVDRQFEDSWA
                     RSDTWITSSRF"
     gene            1829..>2094
                     /locus_tag="VFY27_09820"
     CDS             1829..>2094
                     /locus_tag="VFY27_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5765854.1"
                     /translation="MTFDHTFYEIAALVLLAAAGGLLGLMLRQPLVVSFIGVGILAGP
                     DVLGLATSTEYISLMAEISIAVLLFLVGLKLDIKLVSTLGPVALA"
BASE COUNT          369 a          677 c          632 g          416 t
ORIGIN      
        1 gggcgatggc gactagattc ctcaaacgaa cgccgtagga gcccgccatg cgggcgagtt
       61 agttgagctc ggcaaactcg cccgcgtggc aagctcctac agtctgctgg tgagcctcca
      121 attcatacag tcggacccgt cagaatcgcg aagacgtaat ccacgtatcg gatctcgccc
      181 agctgtcctc gaactgcctg tccacgtcgg cgtcctgccg gtcctgcatt tcgagcgcaa
      241 gtttgaggcc gtagagcgac cagccattgt tcggatggtc ggcgagttcc tcgcggtaaa
      301 cacgctcggc atcggagtga cggccagcct ccagcaacgc agcacccagc cagtgtctcg
      361 ccgcgaacgg cagcggctcg ggttcgtcgt accccagcgc gtcttccagc gcgacggcct
      421 tctcaaacgc ggagatcgcc gcctcgctat cgcccgcgtc gagcagtatc tccccctcca
      481 ggatccgggc tacggcgcct gcaagccgct cggccgggtg gaagcggaac atcgcgtcgc
      541 tggtcgagga gaattcaagt accttctctc gcatcgcttt cgcggttcgc aggtcgccct
      601 ggcgaaggct tgcgtagccc ctggcgaaat cccacagcgc agcgccgact tcgtctgccg
      661 ggcgccgctc gttttccagg atctcgtcga agcggccgaa gcggagcagc gtcagcactt
      721 cgtacatcga attgccggtg atcttgcggt agtccttgcc ggcctgggtg gcgactgcgc
      781 cctgcccgtc gaacgacgcc gcgtacagca gcatatgcag gttgtgcgac ggtccatacg
      841 aaaagccgcg tccctcgccg gctttcaggt cggagtgcca cgccagcgta ttcgcctcga
      901 ccgacttgcc ccacaggccg acttcgttcc aggtgtgcga cggcatgtgc tgcatatggc
      961 ttgccaccgg aaccgcggac gacaggtact ccgcgcacgg cagcgcgagt tccggcttcc
     1021 gggtcgattc cgtggcatgt atgtaaaggt gacaggcacc gggatgcctg ggatcttcct
     1081 cgagcacgcc ggtcaggacg ccgtgcaggc ggaccacgtc gggatcgtcg atatcccggt
     1141 agcctcgacg ctcctcgagc atgaacagcg caaccgcata cacggtggcg acgttatgat
     1201 cgtcgggata cttctcgtag acctgggcca tggcttccgc gaacgccgta tccacggctc
     1261 gcctgttggc cggatcgtag tcttccgggt aacggacgga tgccgccgca atgaggtccc
     1321 gttccacttc gctggtatta gacggagcca actccgccgc gcgcacgata gccgcgtaag
     1381 cgcgttgcga cttctccacc gtcatcgcgc cgttcaggta cgaaccgagc gcgaaggctt
     1441 cgccccagta acacattgca cattgtggat cgagccgctg cgccgcaagc atcgtgcgcg
     1501 ccgcctcatc gacgttgaac gcgtagcgca gctgcattcc ctggtcgaaa tacgcctgcg
     1561 cctggccgct ttcggtgctg atcggccacg agtagggccc gagcgcctca ggtaaaagat
     1621 cgatcggcgc gtccagggct tgcccgcttt catccatcga catcgcggca tgagcggtgc
     1681 ccgcgggcga tgcgctcatc tcattgctcg aagtcgcgca agcggccacg atgcttaagc
     1741 agagcaaaat cgtaaaggta cggttcattc aattctcttg agttgttgac ctgacgtatg
     1801 ctgtttgcag aaacagccgg acgcctcaat gacattcgac cacacatttt atgagattgc
     1861 agcgctggtc ctgctcgctg cggcaggcgg cttgctcgga ttgatgttgc gtcaaccgct
     1921 ggtggtatcg ttcatagggg tcggtatcct cgccggcccg gacgtcctgg gcctcgcgac
     1981 gtcgaccgaa tatatctcgc tgatggccga aatcagcatc gcggtgctgc tgtttctcgt
     2041 tggcctcaag ctcgacatca agctcgttag caccctggga ccggtggcgc ttgc
//
LOCUS       DASUOM010000476         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_211933,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000476 DASUOM010000000
VERSION     DASUOM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211933"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFY27_09825"
     CDS             <1..366
                     /locus_tag="VFY27_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765855.1"
                     /translation="GVEVVRNISAGNSFTETPELSLVVDTPEGQVLLVGCSHPGIERI
                     LESVHRKANSVRLIMGGLHWLTLSDSEVERLARNLEHEWSVHSIAPGHCTGEVGFSVL
                     RRVFGRRYVYAGLGSAIEL"
     gene            617..1300
                     /locus_tag="VFY27_09830"
     CDS             617..1300
                     /locus_tag="VFY27_09830"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX5765856.1"
                     /translation="MRHIDRVLLVGAFGLFCSPAFAEMPKIDWSLEDAIRQIDQQADN
                     FESAMARVQIVRKGADGTVAESVGGDGFINEDGDMRFSQDGGNRVVVVDRKTVSDYDK
                     AAGTVNEYSLSKHKNRLEPYYRFGFSVPVRDMQDRYLFTILGEESVGEMRTLVFELTP
                     TKDDERAVIAKIRLWIDQGSWMPRRQEFSLADRSITTVDYTAVARNLRLNPELFSDSW
                     PRGTKKVKN"
     gene            1324..1992
                     /locus_tag="VFY27_09835"
     CDS             1324..1992
                     /locus_tag="VFY27_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765857.1"
                     /translation="MIPRILLGTASIPGTDKQLQLWQSGDLFSIRIPGRGELMNSRVH
                     GSEKALADLACGKLANEKPRLLIGGLGMGFTLAAALKATGPAAEIVVAELVPEVVTWN
                     RTLIGAPAGNPLADKRCRVHVGDVAAVIRQEAAGFDAILMDVDNGPEALVRQENDWLY
                     SAAGLKATRAALLPRGVLAVWSSGPDRPFGRRLGQAGFDVSEHVVRPHRAGKGPRHYI
                     WIGS"
     gene            2069..2587
                     /locus_tag="VFY27_09840"
     CDS             2069..2587
                     /locus_tag="VFY27_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5765858.1"
                     /translation="MFDNPEIPLEDLPTAEGLQWQPLHDRYVRQLQVMHSFYMLVLIV
                     ALGIFGMTPLAEQLPTGWIWALLTIIAVISLAWPGVDVPRRGWVLREKDIVYRTGVLW
                     QTVTAVPFNRIQHAETSSDPLGRRFGTAALELFTAGGSGGDLRIRGLPAGTAERLREK
                     VMAKIGAAIEED"
     gene            2574..4097
                     /locus_tag="VFY27_09845"
     CDS             2574..4097
                     /locus_tag="VFY27_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5765859.1"
                     /translation="MKRTDAECWQRTSPYSVLFFFGGLVKRLARHATQVIAPIAAFFI
                     AWQGDLQSKIVLAAVALTFMATASALLAYLTFYYRVDEEAIRIRYGVFQKRQLDIRFD
                     RVQSVTTEQNPVYRALGLVSLTFDTAGSSKDEGVLPAIPVAEAEVLRNRIGRVDLVVA
                     DTATPAAAAAPGELLLALGGWDMVRIGLADWRALVLLAVLGPLFERAGNGVTDRIGSR
                     VADSMADVAKGFQEFGVAPGLVVLIALISVLVTLFGLLSIFAAFLRYHRFRLSRDGQR
                     LRSVAGLLTRHENVTHVAKVQILSLRQGLVLRLFRRLRIVAKQARSGSGDRDSRHFQV
                     PLVTPGFAGRFTRLVFDREAQGLDADPWHSAFARVSPHYLRARLLPVALAPAAVACLV
                     IVPGAGWAAAWALLWIPIGALLAFQAWRRTAFRLDRDGIVRRSGLLGVRLDALLFRKV
                     QRVSIRQSWFQRRRRLANLSMFTASGAITLPHIDHATACRLRDYILYRVESSRQAWH"
     gene            complement(4061..5209)
                     /gene="arsB"
                     /locus_tag="VFY27_09850"
     CDS             complement(4061..5209)
                     /gene="arsB"
                     /locus_tag="VFY27_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5765860.1"
                     /translation="MTEPIRRELPFLDRYLTLWIFIAMTIGVAIGYFVDGAPRFVQRF
                     DVGTTNVPIAIGLILMMYPPLAKVRYELLPKVFADTRVLGLSLAQNWLVGPALMFVLA
                     VVFLHGYPEYMVGLILVGIARCIAMVLVWNQLARGNSEYAAALVALNSLFQVVTFGFY
                     AWLFITVLPPLLGLEGSVVDVSVAEIFTSVIIYLGIPFAAGFLSRLVLVRAKGEEWYQ
                     SVFLPRISPLTLVALLFTIVVMFTLQGQAIIDSPLDVVRIAVPLTVYFALMFLLTFYA
                     AWRLGADYSRSTSVAFTAAGNNFELAIAVAIAVFGIESKVAFATVIGPLVEVPVLIGL
                     VHVALYFQRRFHQGAIATIANGPGAAEHASAGPGAPCQCQACRLLSTR"
     gene            complement(5206..5652)
                     /locus_tag="VFY27_09855"
     CDS             complement(5206..5652)
                     /locus_tag="VFY27_09855"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5765861.1"
                     /translation="MEKLKVLFLCTGNACRSQMSEGWARHLRGEHFDTYSAGVSPHGL
                     DPRAVKVMAEAGVDISGHASKHLDTLLGVPFDYVITVCDNAAESCPVFPGTVRKVHRG
                     FEDPPALARTAQTEEEALDHYRRVRDEIRAFVETLPGALTRQDPAT"
     gene            complement(5824..6144)
                     /locus_tag="VFY27_09860"
     CDS             complement(5824..6144)
                     /locus_tag="VFY27_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5765862.1"
                     /translation="MSALAALAHEHRLAIYRLRDEKGPEGLAAGEIAERVGLVPSSLT
                     FHLQHLARAGLLTQRRVGRQLFYATDFGVMNELVGYLTENCCGGAGCAPASGKPRRKP
                     TRVA"
BASE COUNT         1100 a         1928 c         2030 g         1087 t
ORIGIN      
        1 ggcgtcgaag tggttcgcaa tatttctgcc ggcaatagct ttaccgaaac acccgagctg
       61 tcgctggttg tcgacacgcc tgaaggacag gtgctcctcg tgggctgttc gcacccggga
      121 attgagcgga ttctggagtc agtgcatcgc aaggcgaatt ccgttcgtct gatcatggga
      181 gggttgcatt ggctgactct gtccgactcc gaggtggagc ggctggcaag gaatctggaa
      241 catgaatgga gcgttcacag catcgcgccc ggccattgta ccggcgaagt cgggttctcc
      301 gttctacgtc gagtgttcgg acgccggtac gtctacgccg ggttgggcag cgccatcgag
      361 ttgtgagggc agcgcggtgg acgtgaacaa cctgccgcaa gtactgaaca accctgcctg
      421 cgccggttgc ggcccggcaa ggaggcgggg catgctatag tcgtgcgcac ccgccctcac
      481 cctggaactg caggatcact gcagccggga tcccggcatt caggctttcc ccggccgtcc
      541 atgtgaatga cctcgacaag aacaatgcgg tccgcttgga aagacaacca acagacaaga
      601 aaagagggac aaaggcatga gacacataga ccgggtatta ctcgtcggcg cattcggatt
      661 gttctgctcg ccggcatttg ccgaaatgcc gaaaatcgac tggagtctcg aagacgccat
      721 ccggcagatc gaccagcagg cggataactt cgaatccgcc atggcgagag tccagatcgt
      781 ccgtaaaggc gcggacggca cggtcgccga atccgtcggc ggcgacgggt tcatcaatga
      841 ggatggcgac atgcgcttca gccaggacgg cggcaaccgt gtggtcgtcg tcgaccggaa
      901 gactgtctcg gactacgaca aggcagccgg caccgtcaac gaatattcgc tgtcgaaaca
      961 caagaaccgg ctggagcctt actatcgctt cggtttcagc gtgccggtac gcgacatgca
     1021 ggatcgctac ctgttcacga tcctcggcga ggagtcagtg ggcgagatgc gcacgctggt
     1081 attcgagctg acgccaacga aggacgacga acgtgccgtg attgcaaaga tccggctctg
     1141 gatcgaccag ggttcctgga tgccgcggcg ccaggaattc agcctggccg atcgctcgat
     1201 tacgacggtg gattacacgg cagtggcgcg caacctgcga ctcaatccgg aactgttcag
     1261 cgactcctgg ccgcgtggca cgaaaaaagt caaaaactga gcggcggggg agtctcaggt
     1321 ccgatgattc cccgcatcct gctcggcact gcgtcgattc ccggcactga caagcaactg
     1381 cagctgtggc aatccgggga tcttttctcg atcaggattc ccggccgggg cgaactgatg
     1441 aacagccggg tgcacggctc cgaaaaggcc ctcgccgatc tcgcctgcgg aaagctcgcc
     1501 aacgagaaac caagactcct gattggcggt ctcggcatgg gttttaccct ggcggcggcc
     1561 ctgaaagcga ccggacccgc tgccgagatc gtcgtcgcgg agctcgtgcc ggaagtggtg
     1621 acgtggaatc gaaccctgat cggggcgccg gccggcaatc cgctcgcgga caagcgctgc
     1681 cgcgttcatg tcggtgatgt cgctgctgtg attcgccagg aggccgcggg cttcgacgcc
     1741 atcctgatgg acgtggacaa cggaccggaa gcactggtcc gccaggagaa cgactggctc
     1801 tacagcgccg ccgggctcaa ggccactcgc gccgcgctcc tgccccgcgg cgtgctggcc
     1861 gtctggtcct cgggaccgga tcggcccttc ggcaggcggc taggacaggc cggattcgac
     1921 gtgagcgagc atgtcgtgcg gccgcaccgt gccggcaagg ggccccgcca ttacatctgg
     1981 atcggcagct agccgcacag gcgtatgcgc cgctggtcgc gagcggacac agcaggaata
     2041 taatgcgcgc tggtcccgcg gagcgctgat gttcgacaac ccggagatcc cgctcgagga
     2101 tctacccact gccgaaggac tgcaatggca gccactccac gatcgctacg tgcggcagct
     2161 ccaggtaatg cattccttct acatgcttgt tctcatcgtg gcgcttggga tcttcggtat
     2221 gacgccactt gcggagcagt tgccgacagg ctggatttgg gcattgctca ctatcattgc
     2281 cgtcatttcc ctggcctggc cgggtgtcga cgtgccgcgt cgcggctggg tgctgcggga
     2341 gaaggatatc gtctaccgga ccggcgttct ctggcagaca gtcacggccg tgccgttcaa
     2401 tcgcatccag catgccgaga cgtcgagcga cccgctcggc cggcgctttg gtactgcggc
     2461 gctggagctc ttcacggccg ggggctccgg tggcgatctc cgtatccgcg gactgccggc
     2521 cgggacggcc gagcgcctgc gcgagaaggt gatggcgaag atcggagccg cgattgaaga
     2581 ggactgacgc ggagtgctgg cagcgcacgt cgccctattc ggttctgttc ttcttcggcg
     2641 ggctcgtcaa gcggctggcc cggcacgcga cccaggtgat cgctccgatc gccgcgtttt
     2701 tcattgcctg gcagggcgat ctccagagca aaatcgtgct tgcagcggtt gcgctcacgt
     2761 tcatggccac cgcctccgcg ctgctggcat acctcacgtt ctattaccgc gtggacgagg
     2821 aggcgatccg gatccgctat ggagtctttc agaaacggca gctcgatatc cgtttcgatc
     2881 gggttcagag cgttacgacg gagcaaaacc cggtctaccg tgcgctggga ctcgtttcgc
     2941 tgaccttcga tacggccggc tcgagcaaag acgaaggcgt gctgcccgcc atcccggtag
     3001 ccgaggcgga ggttctgcgc aaccggatcg gccgcgtgga tctcgtcgtc gccgacacgg
     3061 cgacaccggc agccgcggcg gcgccgggag aactcctgct ggccctcggt ggttgggaca
     3121 tggtgcggat cgggcttgcc gactggcgcg cgctcgtgtt gctggccgtt cttgggccgc
     3181 tcttcgagcg cgcgggcaat ggcgtaacgg atcgtatcgg ctcgcgcgtc gcagacagca
     3241 tggccgatgt tgcgaagggt ttccaggagt tcggtgtggc gccgggcctc gtcgtcctga
     3301 ttgccctcat cagcgtgctc gtgacgctgt tcgggctcct ctcgatattc gccgcgttcc
     3361 tgcgctacca ccgtttccgg ctgtcacgcg acgggcagcg gctgcgttcc gtcgccggcc
     3421 tcctgacccg gcacgaaaac gtgactcatg tggccaaggt acagatcctg agcctccggc
     3481 aaggcctcgt cctgcggctg ttccggcgtc tgcggatcgt tgcgaagcag gcgcggagcg
     3541 gaagtggcga ccgggacagc cggcatttcc aggtgccgct ggtcacgcca ggttttgccg
     3601 gccgctttac gcgcctcgtg ttcgaccggg aagcgcaggg actggacgcc gacccctggc
     3661 actcagcgtt tgcgcgcgtg tcaccccact acctgcgtgc gcggctgctg ccggtggcac
     3721 tcgccccggc ggccgtcgcc tgcctcgtca ttgttcccgg cgccgggtgg gcagctgcct
     3781 gggcactgct gtggatcccg atcggcgctc tcctggcttt ccaggcgtgg cgacgcaccg
     3841 cgtttcgcct ggaccgtgac ggcatcgtgc ggcgctcggg actcttgggc gtgcggctcg
     3901 acgcgcttct gtttcgcaag gtgcagcgcg tcagcatccg gcaatcatgg ttccagcgcc
     3961 ggcgccgcct cgcgaacctg agtatgttca ctgcgtccgg cgccatcacg ctgccgcaca
     4021 tcgatcacgc gacggcctgc agattgcgcg actacatcct ctaccgggtg gagagcagcc
     4081 ggcaagcctg gcactgacag ggcgctcccg ggccggcact cgcgtgctca gcggcgcccg
     4141 gaccgttcgc gattgtcgcg atcgcgcctt ggtggaagcg gcgctggaag tacagggcga
     4201 cgtgcacgag cccgatcagc accggcactt ccacgagcgg accgatgacg gttgcgaacg
     4261 ccactttcga ctcgatgccg aacacggcga tcgccaccgc gatggccagt tcgaagttgt
     4321 tgccggcggc cgtgaaggcg acgctcgtcg agcgggagta atccgcgccg agccgccagg
     4381 cggcgtagaa agtcaacagg aacatcagcg cgaagtacac tgtcagcggt accgcgatcc
     4441 gcaccacgtc gagcggggaa tcgatgatcg cctgcccctg gagcgtgaac atcacgacga
     4501 tcgtaaagag cagagctacc agggtaagtg gagagatgcg cggcagaaag acgctttggt
     4561 accactcctc gcccttcgcg cggacgagaa ccagcctcga gaggaagccg gcggcaaaag
     4621 ggatgccgag atagattatc acgctcgtga atatttcagc gaccgatacg tccaccacgg
     4681 atccctcgag cccgagaagc ggcggcagca ccgtgatgaa gagccaggca tagaagccga
     4741 acgtaacgac ctggaacagg ctgttgagtg cgacgagcgc ggcggcgtac tcgctgttgc
     4801 ctcgcgccag ctggttccac acgagtacca tggcgatgca gcgggcgata cccacgagga
     4861 tcagtccgac catgtactcc gggtagccgt gcaggaagac gacggcgaga acgaacatca
     4921 gcgccggccc gaccagccag ttctgcgcga gcgacaggcc gagcactctc gtgtcggcga
     4981 aaaccttcgg caggagttcg taacgcacct tcgccagcgg cggatacatc atcaggatga
     5041 ggccgatggc gatcggcacg ttggtggtgc cgacgtcgaa gcgctgaacg aaccggggag
     5101 ccccgtccac gaagtacccg atcgccacgc cgatggtcat ggcgatgaag atccacaggg
     5161 tcaggtagcg atccaggaaa ggcagctcgc ggcggatcgg ttcggtcacg tggccggatc
     5221 ctgtcgggtc agggctccag gaagcgtctc cacgaaggca cggatctcgt cgcgcaccct
     5281 gcgatagtgg tcaagcgcct cctcttcggt ttgcgccgtt cgagccaggg cgggcgggtc
     5341 ctcgaagccg cggtgaacct ttctcaccgt gcccggaaag acgggacagc tctcggccgc
     5401 gttgtcacag acggtgatga cgtagtcgaa cggcacgccg agcagggtat cgaggtgttt
     5461 cgacgcatgg ccggagatgt ccacgcccgc ctcggccatc accttcacgg cgcgcggatc
     5521 gagtccgtgc ggcgaaacgc cggccgagta ggtgtcgaaa tgctcccccc tgaggtgacg
     5581 cgcccagcct tcgctcatct ggcttcggca ggcgttcccc gtacacagga agagcacctt
     5641 tagtttttcc acgccggaaa gctccgcccg atcagccgca gcagatggcg ctgctctcgt
     5701 tggccagttt ggctggtgcg cagcaggagc cttcgccctg cgaggccggc ttgcagcatg
     5761 ccgagggcgg ctcgatgctc ggaggtgtcg atgcacatac ggtccgttcc acaagatcgg
     5821 tcgtcaagcg acgcgcgtgg gcttgcggcg gggtttgccg ctcgcgggcg cacatcccgc
     5881 accaccgcaa cagttctccg tgagatagcc gacgagctcg ttcatgacgc cgaagtccgt
     5941 ggcgtaaaag agctggcggc ccacccgtcg ctgagtgagc aacccggccc gcgccaggtg
     6001 ctgcaggtgg aacgtgagcg aggacggcac gaggccgacg cgctctgcaa tttccccggc
     6061 cgccagtcct tccgggccct tctcgtcccg gagacggtag atggccagcc gatgctcgtg
     6121 ggccaatgcg gcaagcgccg aaatg
//
LOCUS       DASUOM010000477         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_574504,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000477 DASUOM010000000
VERSION     DASUOM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574504"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFY27_09865"
     CDS             complement(<1..705)
                     /locus_tag="VFY27_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765863.1"
                     /translation="MGTRLPTALAAGIATILCVPGAPADQDENPTEFCLHGEFDLGAR
                     LQGLHPAAAEFSAAEWCVITEDESDRVHFRAAGNSNPDLSGEFAVTYLPPHTVRIVNR
                     DAPPDLDFTDAVISAEASRSRRIDPRRVLQELAANPEWVESTSEDGWRTVRYLGESTQ
                     VRLQVLNGRLQALQTWADMPLRGRVPVHWDWDWSDATEPRLTFEVDGEPMFKARGKWR
                     PLDVEQAAAVWKPSGDQ"
     gene            complement(796..2103)
                     /locus_tag="VFY27_09870"
     CDS             complement(796..2103)
                     /locus_tag="VFY27_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5765864.1"
                     /translation="MSTAAAEAVPYSAWRNKRAIWSWSLYDFANSSFTTLIVTFVYAT
                     YFTQAIADDPVHGTTLWSRGVTITALIVAFCSPVLGAIADRGGYRKLFVVVSTLICIA
                     ATAALYTVLPGQVIAALVLVVIANIAYEFATVFYNAFLPDLAPPGRIGTVSGLAWGLG
                     YIGGLLALAFALVALVQPDTPWFGFSKEDGENIRATNLVVAAWFLVFSVPFLLWVPED
                     KSRVSVSGRVIKEAFAQIRHTFVEIRKYRQMVRFLVARLIYNDGLVTVFAFGGIYAAG
                     TFGFSFEEVLLFGIVINVAAGIGAAAMGLLDDKIGGKRTIVISLLGLIGASLLAVLAT
                     SATWFWIAGILLGIFVGPNQAASRSLMGRFVPHEMENEFFGFFAFSGKLTAFIGPLLL
                     GVLTDVSGSQRLGISIVIVLFVLGLFLLLPVDERAGSQFRAPQ"
     gene            complement(2326..3054)
                     /locus_tag="VFY27_09875"
     CDS             complement(2326..3054)
                     /locus_tag="VFY27_09875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5765865.1"
                     /translation="MSSVQEHYDEHLGPVYAWMVGGIGPALERGAAELDAIGAKPSGS
                     GLAVDLGAGFGMHAIPLARRGFDVVAIDSSAVMLEDLSRHAGDVRVQAICGDLLSFPQ
                     HLPAKPELVLCMGDTLTHLPDEEYVERLIGTVRESLAEDGRFVMTLRDYTTPLTGNRR
                     FIPVRSDEQRIFVCFLEYSTARVRVSDILHEREGAQWKMRVSSYDKLRILPEWLEEGL
                     RRAGFAVTREHTASGMLCFVARPA"
     gene            complement(3071..3565)
                     /locus_tag="VFY27_09880"
     CDS             complement(3071..3565)
                     /locus_tag="VFY27_09880"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HEX5765866.1"
                     /translation="MESVLRALLTGAGATAVLDLWSLLLKRFFGVPAPSWNMVGRWVG
                     HFPRGRFKHTSIAAAEPVQGELALGWVTHYVTGIVYGGLLVALWGLDWARQPTLLPAL
                     AVGVATVVAPFFIMQPGMGAGIAGSKMPNPAQVRLRSLMNHTVFGFGLYLAAVLTAML
                     VPAG"
     gene            complement(3620..4204)
                     /locus_tag="VFY27_09885"
     CDS             complement(3620..4204)
                     /locus_tag="VFY27_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5765867.1"
                     /translation="MSARNLAILMLLCFTAPVGVLADELTQMIQQDLVTLGYDPGNTD
                     GEATVQTAVAISKFQAEHDLEVTGEATPQLAGVIKASIDQRHAPAAAIAAAPQATAQP
                     KDPAAQQAAQQACIQEKIEAREASNRKKRGFGSLMRVASRTAARSGGDTATEIASTTH
                     DVYDVSATVSDLESAARDLGITESELEECRNAAQ"
     gene            complement(4292..4612)
                     /locus_tag="VFY27_09890"
     CDS             complement(4292..4612)
                     /locus_tag="VFY27_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX5765868.1"
                     /translation="MIKVSVMYENKPNARFDHAYYKDKHMPLVKKRMGDTCNFYTVDK
                     GVAGGAPGEPATFVAMCHIYCDSVDAFQRGFGPHADEILGDIKNYTDLSPIVQISEVV
                     VGQS"
BASE COUNT          932 a         1546 c         1495 g          873 t
ORIGIN      
        1 ctgatcgcca ctcggcttcc acacagccgc tgcttgttcc acgtcgagcg ggcgccattt
       61 gccgcgtgct ttgaacatcg gttcgccgtc cacttcaaac gtcagccggg gttccgttgc
      121 gtcggaccag tcccagtccc agtgtacggg caccctgccg cgcagcggca tatccgccca
      181 ggtctgtagc gcctggagac ggccgttcag gacttgcagc ctcacttgtg tagattcacc
      241 caaatagcga accgttcgcc agccatcttc tgatgtcgat tccacccact cgggattggc
      301 tgcgagttcc tgcaatacac gcctcggatc aatgcgcctg cttctcgatg cttccgcgga
      361 gatcaccgca tcggtgaagt cgagatcggg cggcgcatca cgattgacga tacgcacggt
      421 gtgcggcggg agataagtga cggcgaactc gccagagagg tccggattgg aattccccgc
      481 tgcacgaaaa tgtacacggt cggattcgtc ctcggtgatt acgcaccact cggcggcgga
      541 gaattcggcc gcagccggat gcaggccttg caggcgggcg ccgagatcga actcgccgtg
      601 gagacagaat tcggtggggt tttcgtcctg atcggcagga gcacccggca cgcacaggat
      661 cgttgcgatg ccggcagcca atgccgtagg tagtcgtgtc cccattcttt ttctcccggt
      721 cgaccgactc cgggtgggac tcgtgtgcga agccgcggtt ccgccaggat gaccgggact
      781 tgcgcttaag ctcctctact gcggcgccct gaactggctg ccggcacgct cgtcaaccgg
      841 caataacagg aagaggccga ggacgaacag cactatcacg atcgaaatcc cgaggcgctg
      901 tgagccggaa acgtcggtca gcacaccgag caacagcggg ccgataaagg cggtgagctt
      961 gccggagaac gcgaagaagc cgaagaattc gttttccatc tcgtgcggta cgaaccgtcc
     1021 catgagcgag cggcttgccg cctggttcgg cccgacgaat atgccgagca atatgccagc
     1081 aatccagaac cacgtcgcgc tggtcgcaag gacagcgagc aagctggcgc cgatcaggcc
     1141 gagcagcgag atcacgatgg tacgcttgcc gccgatcttg tcgtcgagaa gacccatggc
     1201 ggccgcacca attccggcgg cgacattgat gacgatgccg aacagcagca cctcttcgaa
     1261 gctgaagccg aacgtgcccg ccgcgtagat gccgccgaag gcgaacacgg tgacgaggcc
     1321 gtcgttgtag atgaggcgcg cgaccaggaa gcgcaccatc tggcgatact tccgtatctc
     1381 gacgaacgta tgcctgatct gcgcaaacgc ttccttgatc acgcggccgg aaaccgatac
     1441 ccgcgacttg tcttccggca cccacagcag gaacggcacg ctgaacacca gaaaccaggc
     1501 cgcgacgacc aggttcgtcg cacgaatgtt ctcgccgtct tccttcgaga aaccgaacca
     1561 cggcgtgtcc ggctgcacca gcgctaccag tgcgaacgcg agcgcaagca agccgccgat
     1621 gtagccgagt ccccaggcaa ggccggagac ggttccgatc ctgcccggtg gcgccaggtc
     1681 cggcaggaac gcgttgtaga agacggtggc aaactcgtag gcgatgttcg cgatcacgac
     1741 gagtacgaga gcggcgatga cctgccccgg caataccgtg tacagcgccg ccgttgcggc
     1801 gatgcaaatg agcgtcgaca cgacgacgaa cagcttgcga tagccgccgc gatccgcaat
     1861 cgcgccgagc acgggcgaac agaacgcgac gatgagcgcc gttatcgtca cgcctcgcga
     1921 ccagagggtc gtcccgtgca ccggatcgtc cgcaatcgcc tgcgtgaaat aggtcgcgta
     1981 gacgaaggtg acgatcagcg tggtgaacga ggagttcgcg aagtcgtaca gcgaccagga
     2041 ccagatggca cgtttgttcc gccacgcgct gtacggcacg gcctcggccg ccgccgtact
     2101 cacagtgtgc gcatccgtgg cagagcaaag tgactcgaag tcaggcgtcg cgcgatcatt
     2161 catcgatccc tttttctaat ggacgcgaaa atagcagctc tacctggaga ctacgacgga
     2221 atccgacgcc ggccgtcctt cggagcgaca gccatgcatc ttcgcgaata cttctcattc
     2281 aggaaatccc tccgcggacg cgaccgtagc gatgccgtgg cgcgttcagg ccggccgtgc
     2341 gacgaagcac agcatgccgg atgcagtgtg ctcacgcgtc accgcaaagc cggcccggcg
     2401 caggccttcc tccagccact ccgggaggat gcgcagcttg tcgtaactgc tgactctcat
     2461 cttccactgc gcaccttcgc gttcgtgcag gatgtcgctg acacgcacgc gggcggtgga
     2521 gtactcgagg aagcagacga agattcgctg ctcgtcgctg cgcaccggga tgaagcgccg
     2581 gttcccggtc aagggcgtgg tgtagtcgcg caacgtcatg acgaagcggc catcttcggc
     2641 gagcgactcg cggacggtgc caatgagccg ctcgacatat tcctcatccg gcaggtgcgt
     2701 cagcgtgtcg cccatgcaaa gcacgagctc cggcttcgcc ggcaggtgtt gcggaaacga
     2761 cagcaggtcg ccgcagatgg cctgcacgcg cacatcaccc gcgtgccgcg acaagtcctc
     2821 gagcatcacg gcggaagaat cgatggcgac gacgtcgaaa ccgcggcgcg ccagcgggat
     2881 agcgtgcatg ccgaagcccg cgccgagatc caccgcgaga ccgctaccgg atggcttcgc
     2941 gccgatcgca tcgagttccg ccgcaccacg ctcgagcgca ggtccgatgc cgcccaccat
     3001 ccacgcatac accggcccca ggtgttcgtc gtaatgttcc tgaaccgagg acatgctcct
     3061 acagcgggta tcagccagcc ggaaccagca tcgcggtaag gaccgcggcc aggtagaggc
     3121 cgaaaccgaa gaccgtgtgg ttcatcaggc tgcgcaggcg cacctgtgcc gggttcggca
     3181 ttttcgagcc tgcaatgccc gcccccatgc cgggctgcat gatgaagaag ggagccacca
     3241 ccgtggcgac gcccacggcc agggccggaa gcagcgtcgg ctggcgcgcc cagtcgagtc
     3301 cccacagcgc caccagcagc ccgccgtaca ctatgcccgt tacgtagtgg gtgacccagc
     3361 cgagagcgag ttcgccttga accggctccg ccgccgcgat actcgtgtgt ttgaagcgcc
     3421 cgcgtggaaa atggccgacc cagcgcccga ccatgttcca gctgggcgcc ggaacaccga
     3481 agaagcgctt gagaagcagg ctccagagat cgagaacagc cgtggcgcca gcgccggtca
     3541 gcaaggcgcg cagcacgctt tccacacctt ccgccatgcg ctttttgggt acggcgtcag
     3601 ctgcaggtcg cgattaccgc tactgcgcag cgttccggca ctcttccagt tcgctttccg
     3661 tgatgccgag gtcgcgggct gccgattcga gatcggatac ggtggcgctg acgtcgtaaa
     3721 cgtcgtgtgt ggtgctggca atctcggtgg cggtatcacc gcccgagcgc gctgccgtgc
     3781 ggcttgccac cctcatcagg ctgccgaagc cgcgcttctt ccggttcgac gcttcccgcg
     3841 cttctatctt ctcctggatg caggcttgct gcgcagcctg ctgcgccgcc ggatccttcg
     3901 gctgggccgt tgcctgcggg gccgcagcaa tcgcggcggc aggcgcatgc cgttgatcga
     3961 tagaggcctt gattacgccg gcgagctgcg gcgtggcctc tcccgtcacc tccaggtcgt
     4021 gttccgcctg gaatttggag atggcgacgg ccgtctggac ggtcgcttcg ccatccgtat
     4081 tcccggggtc atagcccagg gtaaccaggt cctgctggat catctgtgtc agttcatcgg
     4141 cgagaacgcc gaccggcgca gtgaagcaga gcagcatcag tatcgccaag tttcttgcag
     4201 acataggtta ggtctccggt gaaacccgct ttgttgttgt atctcatgag aatagcagac
     4261 atatcgacgc cagcatcggg taaaccaggg gttagctttg gccgacgacg acctcgctga
     4321 tctgcacgat cggcgaaagg tccgtgtagt tcttgatgtc gcccaggatt tcgtccgcat
     4381 gcgggccgaa accgcgctgg aacgcgtcga cggagtcgca gtagatgtgg cacatggcga
     4441 cgaaagtcgc aggctcaccc ggagcgccac ccgccacgcc cttgtcgacc gtgtagaagt
     4501 tgcaggtatc gcccattcgc ttcttgacga gcggcatgtg cttgtccttg tagtacgcat
     4561 ggtcgaaccg cgcgttgggc ttgttttcgt acatcacact taccttgatc atgcgttttt
     4621 cccctggtta cgagtatggg caacgaacgg ctgccacttc ggtcctgcag cccggtgatt
     4681 ataaacgggt ccggtgagta gcgggcttag cgcagcttcg cgacaagcaa gcgcgtcagt
     4741 tcgaggacat ttggcttcac ggcctcaacg tcgtcgtccg cgtccgcctg caacgtcaat
     4801 agctgctcgc cgactttcac gtagacctct gcgtattgat ctgcgt
//
LOCUS       DASUOM010000478        11104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1554061,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000478 DASUOM010000000
VERSION     DASUOM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554061"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFY27_09895"
     CDS             complement(<1..617)
                     /locus_tag="VFY27_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX5765869.1"
                     /translation="MDLKRRQFLGGMAAGSAILTMPAFLSGCGVSPAVTPATPAPENP
                     FLTWFGVDEAAIAQVMAELAANGADAADLYFQHTRVNFLGMEDGIVSRADSEISQGVG
                     LRAVVGDQTGYAFTEDLTMPSMLAAARTAAAIASGSRVVPPQAYNPKNSGDLYRTQVA
                     WADVGVESKLPLLQRVDRLARAADPYVDKVSVYWADSDERVLIAT"
     gene            complement(768..1403)
                     /locus_tag="VFY27_09900"
     CDS             complement(768..1403)
                     /locus_tag="VFY27_09900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5765870.1"
                     /translation="MNQYSHQRRGPADTSAIPNVIFVLLITNGLIYAAQQLAPDFMLT
                     HFPLWPLASANPPFRPWQLVTYGFLHDMFGLSHILFNMFGLWMFGRDLERLMGARRFL
                     TYYLTCVVGAGVVQLVVAAMQGGNYPTLGASGGVFGILLAYGMAFPNRTVLLLIPPIP
                     MKAKYFVLAYGLLELFLGVSGLSPGVANFAHLGGMLFGFILLQYWRGQRRR"
     gene            complement(1481..3175)
                     /locus_tag="VFY27_09905"
     CDS             complement(1481..3175)
                     /locus_tag="VFY27_09905"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX5765871.1"
                     /translation="MSRVAGIFLLLVCTVAVGDERILNYHSEIVVRQDGWIEVTETIS
                     VRAEGKEIRRGIYRDYPTRYRDDFNNRLEVRYEPRSLLRDGQAESFHTEERANGVRAY
                     FGSADRRLSPGEYTYTWHYDAGRMLGFFEGHDELYWNVTGLGWQFPIDRASATVSFDF
                     DLPPRAVSAEAYTGTFQSRGEDYTASIDSGRAQVETTRPLGAGEGLTIAVAWPKGYVV
                     EPSRMQRAKWLLTDNANLLVALGGLLAILAYYIPVWRRFGRDPEEGLIVTRYKPPEGF
                     SPASLRYIYRMGYDNKAMTAAVVNLAVKGYLRIEKSGKTHVLHRLDEGPGAAPLAAGE
                     KALLSQLFRDGSRLELDAGNHKLLGKAMEKHRTSLKRDYANRYFRTNGAMNLPPILIA
                     FASSVIAFAVGGPTPFVIGLVILTAAGIVVFAILMKRVTGLGRKVLDQAAGFREYLEV
                     AEKEEMNLKNPPEKTPELFERYLPFALAMDVEQLWAERFAEVFANLRRAGGAPYHPGW
                     YNGAWDVNNFSRATSSMTSGLSSAISSSVTAPGSSSGSGGGGFSGGGGGGGGGGGW"
     gene            complement(3180..3746)
                     /locus_tag="VFY27_09910"
     CDS             complement(3180..3746)
                     /locus_tag="VFY27_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5765872.1"
                     /translation="MLYSVGGILLVLALCIAVIYNRLVNDRNRVNGAWSDIDVQLQRR
                     HDLIPQLVKAVDQYARYERATIEAVTALRAEAMRTTDIEKLGKAEEELSSGVQRLLAV
                     AEQYPDLKANENFLSLQAELVETEDYLQFARRYYNGSVREFNTRMETVPNNIVAGWFG
                     FTSRSFFQKSSDDVAAVPLVEMGRIERG"
     gene            complement(3753..4193)
                     /locus_tag="VFY27_09915"
     CDS             complement(3753..4193)
                     /locus_tag="VFY27_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5765873.1"
                     /translation="MGRLAGIARRERKRAPMQTLERAEISTESGVAHDFRGRPGPRQV
                     TLISAAAWRAACAELKDDLPWTTRRANLLVEDFELPKTIGAVLRVGAVRLCITGEVDP
                     CSRMEEQHPGLKAALQPDWRGGVSCTVITGGPVSLGDEVELEAG"
     gene            complement(4282..5445)
                     /locus_tag="VFY27_09920"
     CDS             complement(4282..5445)
                     /locus_tag="VFY27_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5765874.1"
                     /translation="MPVRLQTFLDYGFRPFFLLNGIFAVVVVVLWTLLLHGAWTSGPG
                     NFALWHAHEMLVGFAMATIAGFLLTAIPNWTGRERLNGHLLGWLVLAWLCGRAAMAAS
                     GMLPAWFVAPVDLAFPLLLFLFVLQEIVSAGNRRNYPIIAITAVLLLLNLLYHLGAAG
                     RVPGGGRAAIYLMTHTILLLITVIGGRVIPNFTANWMRNHNIARVPANDTTVDRLTIL
                     VTVLVGLASSFSQAGTLTGSLALAAAVLHGVRLAAWRGLATRTEPLLLVLHVAYAWLP
                     LGYALMACAAFGFLVPPTAALHALTVGAIGTMILAMMTRVPLGHTGRPLHASRLTVMA
                     YVVLTLAVIIRVAGPLAGPAYPRMIDASAAAWVLAFAIFSFQYWPILTRPRVS"
     gene            5594..6007
                     /gene="rnk"
                     /locus_tag="VFY27_09925"
     CDS             5594..6007
                     /gene="rnk"
                     /locus_tag="VFY27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX5765875.1"
                     /translation="MGTAPKVVISTRDAERLEALLQSMPGKAFPGKAELEAELDRADV
                     VEPQEIPPTVVTMNSTVKFRVHSSQEEFQLTLVYPKDVDQSGGTISVLAPVGSALLGL
                     SQGDEIEWPKPGGGTIQVRIEEILYQPERAGEFHR"
     gene            complement(6050..7189)
                     /locus_tag="VFY27_09930"
     CDS             complement(6050..7189)
                     /locus_tag="VFY27_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX5765876.1"
                     /translation="MPATSTSYKLIAVCLVAAAQGVVAGVLPEDRTDVLYHLYDGGGV
                     QIDGPSLLMRKQLGKNVSVVGNYYVDMVSSASIDVITTASPYTEEREQYSLGMDYLHG
                     NTSMSLGYTSSVESDFEATTYNLSISQDMFGDLTTLTLSYALGDDVVGRSDDEAFSRD
                     NTRQHYGIGLTQILTRNLITTLNFETITDEGFLNNPYRSVRYADPGSALGYSYEAELY
                     PHTRTSNAVGLRARYHLPYRAALQGEYRFFTDTWDIESHTAAISYVHPMDDWTFTVKY
                     RWHDQTGAHFFSDLFPRSEATNFRGRDKELSPLTSQTLKLAASYEILSDGWRFIDKGS
                     INFSIDFLTVDYHEFRDLTAVALPGEEPFYGLEADVVQIFFSFWY"
     gene            complement(7207..7443)
                     /locus_tag="VFY27_09935"
     CDS             complement(7207..7443)
                     /locus_tag="VFY27_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019674987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEX5765877.1"
                     /translation="MNANRICRLLAPLVIAAGVSACGSIEPWVKPYERDRLADPIMFL
                     DADPVSSSYIQHVYEAREGARGGEGAAGGGCGCN"
     gene            complement(7508..8194)
                     /locus_tag="VFY27_09940"
     CDS             complement(7508..8194)
                     /locus_tag="VFY27_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX5765878.1"
                     /translation="MESWLRVLFLALVVSGMSGCAATKNLFGMGEDQRASPPAEAPTP
                     EQVIDPQVERRDIREPDIDSENFEIGAFAGVMSIEDFGSDVVYGARLSYHITEGLFVE
                     GTVGQSEAGLTSFEELSGGAPILSDTDREFTYYNLSLGYNLFPGEVFLGEGRAFNTSL
                     YLIGGLGSTRFAGDDRFTANVGAGYRFLISDWMALHLDFRDHMFDTDLLGTEKTAHNF
                     EAHLGMTVFF"
     gene            complement(8164..8889)
                     /locus_tag="VFY27_09945"
     CDS             complement(8164..8889)
                     /locus_tag="VFY27_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5765879.1"
                     /translation="MAAVLCQTFFAGLAWADGDYRTVSVADPYLEMHTGPGRGYPKFH
                     VVDRGEVVEVIKRRTDWFLVRDAGGDEGWVDRAQMELTLQPDGQEIKFSEAGQEDFTN
                     ARWEGGVLGGDFGGANIISVYTGYSLNPNVAVEVWGSQILGNFSNGWMASVNVVHETW
                     PEWRVSPFFTLGAGVLHTAPKSTIVQGEDRTDQVAHVGGGLRIYATRRFMVRAEYKSY
                     VVFTSRDDNEEVEEWKVGFAFFF"
     gene            complement(8903..>11104)
                     /locus_tag="VFY27_09950"
     CDS             complement(8903..>11104)
                     /locus_tag="VFY27_09950"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765880.1"
                     /translation="GELRRVIASDLTAEIGHDVAPHFLPDGRVIFSSTRQLRSSAVLL
                     DEGKPQFPALDEDANEMAFVLHVMDADGSNIEQVSYNQSHDFDPSVMSNGQIVFSRWD
                     HMGVNDSVNLYRMNPDGSALELLYGQNSHDSGTDGATVQFMQPRELEDGRIMALVRPY
                     SDTDAGGDIMVIDTPAYLEITQPNKDNAALTGPAQEPATVLEVSTLPNALSSGGRFRS
                     VFPIQDGTGRLLTSWNQCRVTEEMLNLPCTADRLDDPAAEAAPPIYGIWIYDPRDNTQ
                     LPVVPPEEGFIFTEAVSADPRAAPPVILDNENTFTPDPDLVGEGAGVISIRSVYDIDG
                     GATANIEALADPAQTMADQRPARFLRVVKAVSIPDEDIVDLDGTAFGRSTQQGMKEIV
                     GYAPIEPDGSVMMKVPANVALAVSVLDADGRRISGRHQNWLQVRPGQLLECNGCHMPQ
                     SGVSHGRSDAFESVWAGAPTAGTTFPNTDPALFVGEVGETMAEVRARITCGASNCSSL
                     EPSVDLVYEDVWTDEVAAGRPKDASFDYSYADLTTPVPTSLDCMQQWSANCRIVINYE
                     THIHPLWAAPRPVLDDMGNPVLDGSGFPLSNDCTNCHTPIDDQGGVRVPAGQLDLSDG
                     LSPDEPVHFNSYRELLFPDDAQEGGMGALQDVLVQVGIDPDTGDPLFATIPVGPSMNV
                     AGANASEQFFDRFDAGQSHDGYLSPAEKRLIAEWLDVGAQYYNNPFDVPQN"
BASE COUNT         2172 a         3456 c         3357 g         2119 t
ORIGIN      
        1 aaagtcgcga tcagtacccg ctcgtccgag tcggcccaat acaccgaaac cttgtctacg
       61 taaggatcgg cggccctcgc caagcgatcg acccgctgca acagcggcag cttgctttcc
      121 acgccaacat cggcccacgc aacctgcgtc cggtaaaggt cgcccgagtt cttcgggttg
      181 tatgcctgcg gcgggacgac cctcgacccg gaagcgatcg ctgccgcggt cctggcggcg
      241 gccagcatcg acggcatcgt caggtcctcg gtgaatgcgt agcccgtctg gtcacccacc
      301 acggcgcgga ggccgactcc ctgggagatc tccgagtctg cgcggctgac gatgccatct
      361 tccatgccga ggaagttgac ccgcgtatgc tggaagtaga ggtctgcggc atccgcgccg
      421 ttcgccgcaa gttcggccat cacttgcgca atcgccgcct cgtccacgcc gaaccaggtg
      481 agaaaaggat tctccggtgc cggtgtcgct ggcgtcaccg ccgggctcac gccacagccg
      541 gagaggaatg ccggcatcgt caggatggca ctgccggcag ccatgccgcc cagaaactgc
      601 ctgcgcttca aatccattat cgctcccgtt gttcttcgac ttcatgttcc gccagggaac
      661 ccctattaaa cccgagatgc gactcgacgg aaaacggcgg caggtcaaat cccgaccgcg
      721 atcacagaaa taatcccggg acgcgccaca tcctcccgcg agtgcggtca gcgccgccgc
      781 tggccgcgcc agtactgcaa gagaatgaag ccgaacagca tgccgcccag gtgggcgaag
      841 ttggcgacgc cgggtgaaag gccgctgacg ccgaggaaca gttcgagcag cccgtatgcc
      901 aggacgaagt acttggcttt catcgggatc gggggaatga gcagcaggac agtgcgattc
      961 gggaatgcca tgccgtaggc aagcaggatg ccgaatacgc cacccgaggc accgagcgtg
     1021 gggtagtttc ctccctgcat ggctgcgacc accagttgca ccacgccggc gccgaccacg
     1081 caagtcaggt agtaggtgag aaagcgccgc gctcccatga gccgctcgag atccctgccg
     1141 aacatccaca gcccgaacat gttgaagaga atgtggctca ggccgaacat gtcgtgcagg
     1201 aagccatagg tgacgagttg ccacggcctg aagggcggat tcgcggaggc gagcggccac
     1261 agcggaaagt gcgtcagcat gaagtcgggc gccagttgct gcgccgcata gataaggccg
     1321 ttcgttatga gcagcacgaa aatgacgttc gggatcgcgc tggtgtcggc cggacctcgt
     1381 cgttgatggg aatactggtt caaaccgtgt ccttgtgctt cggccgatgc ggccgtagat
     1441 gttaaatgac tgtgcggcgc gctggctgaa tcggtctgaa ctaccagccg ccgcctccgc
     1501 cgccgccacc tccgccaccg gagaaaccgc caccgccgct gcctgaggaa gatcctggcg
     1561 ccgtgaccga ggagctgatc gcgctactca agccgctggt catgctcgat gtcgcgcgac
     1621 tgaagttgtt gacgtcccag gcgccgttgt accagccggg atgatacgga gcgccgcccg
     1681 cccggcgcaa atttgcgaag acttcagcaa agcgttcggc ccagagttgc tccacatcca
     1741 tggcaagggc gaaaggcagg taacgctcga agagttctgg tgtcttttcc ggcggattct
     1801 taaggttcat ctcttccttc tcggcgactt ccaggtactc ccggaaacct gctgcctggt
     1861 ccaggacttt tcggccgagt cccgtgacgc gcttcatcag gatcgcgaaa acgacgattc
     1921 cggcagccgt caggatgacg agcccgatga cgaacggtgt tggcccgccc actgcgaagg
     1981 caataacgga agaagcaaac gcgatgagga ttggtggcag gttcatggcg ccgttggtcc
     2041 tgaaatagcg gtttgcataa tcccgtttca acgacgtccg gtgcttctcc atggctttcc
     2101 ccaaaagctt gtggttgccg gcatccagtt cgagacggct tccgtccctg aacaactgcg
     2161 agagaagcgc cttttccccc gcagccagcg gcgctgctcc ggggccttca tcaaggcgat
     2221 gcagcacgtg ggtcttgccg gacttttcga tgcgcaggta acccttgact gcgagattga
     2281 ccaccgccgc ggtcatcgcc ttgttgtcgt agcccatgcg atagatgtat cggagcgacg
     2341 cgggcgagaa cccctccggc ggcttgtacc gggtcacgat cagtccttct tccggatcgc
     2401 gcccaaaccg ccgccatacg ggtatgtagt aggccagaat cgcaagcagc ccgccgagcg
     2461 cgaccaacag attggcatta tcagtgagca accattttgc ccgctgcatt cgggaaggct
     2521 cgacgacgta ccccttgggc caggcaactg cgatcgtcaa tccctctccc gcaccgagcg
     2581 gccttgtcgt ttctacctgc gcgcggccgg aatcgatgga agcagtgtag tcctctcctc
     2641 tcgattggaa ggtgccggta taggcctccg ccgaaaccgc gcggggcggc aagtcgaagt
     2701 cgaagctgac cgtcgccgag gcacgatcaa tggggaactg ccatcccagt ccggtgacat
     2761 tccagtacag ttcgtcgtgc ccttcgaaga acccgagcat cctcccggcg tcgtagtgcc
     2821 aggtataggt gtattcgccg gggctcagcc gccggtctgc cgagccgaag taggcccgaa
     2881 cgccgtttgc gcgctcctcg gtgtgaaagc tttcggcctg gccgtctcgc aggagcgagc
     2941 gtggttcgta gcggacctcg agcctgttat tgaaatcgtc cctgtagcgg gtcggatagt
     3001 cgcgatagat cccgcgccga atctcctttc cctccgccct gaccgaaatc gtttcggtca
     3061 cctcgatcca gccgtcctgc cgcaccacga tttcgctgtg atagttgagg attctctcgt
     3121 cgcccaccgc gacggtgcaa acgagcagaa gaaaaatacc cgcgaccctg ctcacccggt
     3181 caccctcgtt ctatccggcc catctccacc agcgggaccg cggccacgtc gtcggaactc
     3241 ttctgaaaaa agctgcgcga cgtaaagccg aaccagcccg ctacaatatt gttcggcacg
     3301 gtttccatgc gtgtgttgaa ctcacgcacc gagccgttgt agtagcggcg cgcgaactgc
     3361 aggtaatctt ccgtttctac gagctcggcc tggaggctca ggaagttctc attcgccttt
     3421 agatccggat actgctcggc gaccgcgagc aggcgctgga caccactgct gagttcctct
     3481 tccgctttcc cgagtttctc gatatcggtc gtgcgcattg cctcggcgcg caaggcggtg
     3541 actgcttcga tcgtggcccg ttcgtagcgg gcatactggt cgacagcctt caccagttgc
     3601 ggaatgagat cgtgccggcg ctgcagttga acgtcgatat cgctccatgc gccgtttacg
     3661 cgatttcgat cgttcacgag gcggttgtat atgaccgcta tgcaaagagc gagaacaagc
     3721 aggattccgc cgaccgagta gagcatgctg attcagcctg cctccagctc gacttcatcg
     3781 cccagcgaca ccgggccgcc cgtaatgacg gtgcaactga cgccgccgcg ccaatcgggt
     3841 tgtaaggctg ccttcagacc cggatgttgt tcctccatgc gggagcaggg atcgacctcg
     3901 ccggttatgc aaagacgcac tgcgcccacc ctcagtacgg ctccaatggt ttttggcagc
     3961 tcgaaatcct cgacaagcag gttggctcgc cgggtggtcc acggaaggtc gtccttcagt
     4021 tcggcacagg ccgctcgcca cgccgcggca gaaatcagcg ttacctggcg cggtcccggt
     4081 ctgccccgga aatcgtgggc gacgccggat tcggtgctga tctccgctcg ctccagtgtt
     4141 tgcattggcg cccgcttcct ttcccggcgc gcgatccccg ccagacgacc catgtcactt
     4201 cctccgcaat ggacggtgcg cccgcccggg atgcgggatt gtaccgcaga ggccgtagcg
     4261 actattcctg gattgtccgg cctatgacac tcgaggccgg gtcaggatgg gccagtactg
     4321 aaagctgaag atggcgaagg cgagaaccca cgccgcggcc gaggcatcga tcattcgcgg
     4381 gtacgccggg cccgcgagcg gtcctgcgac ccggatgatg acagcgaggg taagcacgac
     4441 ataggccatc actgtcagtc gcgacgcgtg cagcggcctg ccggtatggc ccagcgggac
     4501 gcgcgtcatc atggccagga tcatcgtgcc gatcgcaccg accgttaacg cgtgcagtgc
     4561 agcggtcggt ggaaccagga agccgaatgc agcgcacgcc atcagcgcgt agccgagcgg
     4621 aagccacgca tatgcgacgt gcaggaccag gagcaggggc tcggtgcggg tcgccaatcc
     4681 tcgccacgca gccaggcgca cgccgtggag gacggcggcg gccaatgcga gcgatcccgt
     4741 tagcgtgccg gcttgcgaaa aactcgacgc gagaccgacg agcacggtta cgaggatggt
     4801 cagcctgtcg acggtcgtat cgttggcagg aacgcgtgca atgttgtgat tgcgcatcca
     4861 gttcgccgtg aagttcggaa tgacgcgacc cccgatgacc gtaatgagca gcagtatcgt
     4921 gtgcgtcatg agatagatcg ctgcgcggcc gccgccgggc acgcgtccgg cggcacccag
     4981 gtgatacagg aggttcaaca acagcagaac cgccgttatc gcgatgatcg ggtaattgcg
     5041 ccggttgccg gcgctcacga tctcctgcag gacgaaaagg aaaagcaaca gcggaaaggc
     5101 caggtcgacc ggcgcaacga accacgccgg cagcatgccg gacgctgcca tcgcggcgcg
     5161 accgcagagc caggcgagaa cgagccagcc aaggaggtgc ccgttaaggc gttcacgacc
     5221 ggtccagttg ggaatcgcgg tgagaagaaa ccccgctatc gtcgccatcg cgaacccgac
     5281 cagcatctca tgcgcgtgcc atagcgcaaa atttccaggg cctgatgtcc atgcgccgtg
     5341 cagcaacagg gtccacagga ccacgacgac gacggcgaaa atgccgttca acaggaaaaa
     5401 cggccggaat ccatagtcga gaaaagtttg caggcgaaca ggcatgatct gagatcctgc
     5461 gttgccaggg gcaacggtgc agtgaatatc cctcgcaagg atgccttacg cgggaacacg
     5521 cgtcgagctg cctatccggc tggtcaatcc tctatactaa gcattggatg cgcaaaaccg
     5581 accggggaaa gcagtgggca cggcaccgaa agtagtgata tcgacacgtg acgcagagag
     5641 gttggaggcc ctcctgcaat caatgccggg gaaggcattc cccggcaagg ctgaactcga
     5701 ggccgagctg gatcgagccg atgtggtcga gccgcaggaa ataccgccga ccgtcgtcac
     5761 gatgaattcc accgtgaagt ttagggttca ttcgtcacag gaagaatttc agctgacgct
     5821 ggtgtacccg aaggatgtcg accagagcgg cggaaccatt tccgtcctgg ctccggttgg
     5881 cagcgcgctg ctcggactct cccagggcga cgagatcgaa tggccgaagc cgggcggcgg
     5941 cacaatccag gtccggatag aggaaatcct gtaccagccg gagcgcgccg gcgaattcca
     6001 ccgctagcga gccgggggac agttcaatca aatctgtccc cttactacgc tagtaccaga
     6061 acgagaagaa gatctgtacg acgtcggctt cgagtccgta gaacggctcc tcgccaggca
     6121 gcgcgactgc cgtcaaatcg cgaaactcgt ggtaatccac cgtcaggaag tcgatggaga
     6181 agttgatcga acccttgtcg atgaaccgcc atccgtcact tagaatttcg tagcttgcgg
     6241 ctagtttcaa tgtctggctc gtcagcgggc tcagttcctt gtcgcggccg cggaagttcg
     6301 ttgcctcgga gcgtggaaaa agatcgctga agaagtgagc gccggtctgg tcgtgccagc
     6361 gatactttac cgtgaaagtc cagtcatcca tcggatggac gtacgaaatc gctgcggtat
     6421 gcgattcgat atcccaggtg tcggtgaaga acctgtattc cccctgcaac gccgcccgat
     6481 agggaaggtg atatcgcgct cgcaagccca ccgcatttga ggtgcgcgta tgaggataca
     6541 gctcggcttc gtagctatag cccaatgcgc tgcctgggtc tgcatagcgg acggagcgat
     6601 agggattgtt gaggaaacct tcgtccgtga tggtctcgaa gttcagggtc gtgatgaggt
     6661 ttcgtgtcag gatctgcgtg aggccgatgc cgtaatgctg ccgcgtgttg tcccttgaga
     6721 acgcctcgtc gtcggacctg ccgacgacgt cgtcaccgag agcatagctt aaggtgaggg
     6781 tcgtcaagtc gccaaacata tcctggctga tcgacaggtt gtaagtcgtg gcctcgaaat
     6841 cgctctcgac actgcttgtg tacccgagac tcatgctcgt attgccgtga aggtagtcca
     6901 tgcccagcga atattgctcc cgctcctcgg tgtaggggct tgccgtggta atgacatcga
     6961 tcgacgccga actgaccata tcgacgtagt agttaccgac gaccgacacg ttcttcccta
     7021 gctgcttgcg catcaggagg gagggcccgt cgatctgcac gccgccgcca tcgtacaggt
     7081 ggtaaagaac gtcagtgcga tcttcgggaa gcacaccggc aacaaccccc tgtgccgcgg
     7141 ccacgaggca gactgcgatc agtttgtatg aagtgcttgt ggcaggcatc gcgatacgcc
     7201 cgagcgtcag ttgcaaccgc agccgccgcc ggcggcgcct tcgccgcccc tggcgccttc
     7261 gcgcgcctcg tacacgtgct gtatataaga ggaggaaacg ggatcggcgt ccaggaacat
     7321 gatcgggtcc gccagccggt cacgctcgta aggtttgacc cagggctcga tgctgccaca
     7381 ggccgataca cctgccgcta taacaagcgg cgcgagtaga cggcaaatcc gatttgcatt
     7441 catatccctg ttcctgtctc tatccaaaaa ttagcgatac gccgcgggat cgaccgtgac
     7501 gcctgcctca gaagaacact gtcatgccga ggtgcgcttc gaagttgtgc gccgtctttt
     7561 ccgtgcccag caagtccgta tcgaacatgt ggtcacggaa atcgaggtgc agcgccatcc
     7621 agtcgctgat caggaaacga tagccggcgc cgacgtttgc agtgaacctg tcatcgcccg
     7681 caaagcgagt cgagcccagc ccgccgatga ggtaaaggct cgtattgaac gcgcgcccct
     7741 cgcccagaaa cacctcaccc ggaaacaggt tgtagccgag actcaggttg tagtaggtaa
     7801 attcccgatc ggtatcggaa aggatcggag cgccgccgct cagctcctcg aagctggtca
     7861 agcctgcctc ggactgtccc acggttccct cgacgaacag gccttcggta atgtgatagg
     7921 aaagccttgc cccgtagacc acgtcggagc cgaagtcctc gatgctcata accccggcaa
     7981 aagcgccgat ctcgaagttc tcggaatcga tatccggttc gcggatatcg cgccgctcga
     8041 cctgcgggtc gatgacctgc tcgggcgttg gcgcctccgc cgggggcgat gcccgctggt
     8101 cttcgcccat gccgaacagg ttcttggtcg cagcgcagcc gctcatgccg gacacgacca
     8161 gtgctaaaaa aagaacgcga agccaacttt ccattcttct acttcctcgt tgtcgtcacg
     8221 gctcgtgaac accacgtagc tcttgtattc cgcccgcacc atgaaccgcc tcgttgcgta
     8281 gatgcgcagg ccccctccca cgtgcgcgac ctggtcggtg cgatcttctc cctggacgat
     8341 cgttgatttc ggcgccgtgt gtagaacgcc ggcgccgagc gtgaagaacg gggataccct
     8401 ccattccggc caggtttcgt ggacgacgtt aacactggcc atccagccgt tcgaaaaatt
     8461 tcccagaatc tgtgaacccc atacctccac ggccacgttc gggttgagtg aatagccggt
     8521 atagacggaa atgatgttcg cgcctccgaa gtcgcctccc agcacaccgc cttcccagcg
     8581 ggcattcgtg aaatcctcct gccccgcctc cgaaaacttg atctcctgtc cgtccggctg
     8641 caacgtcagc tccatctggg cgcgatctac ccaaccctcg tcgccgcctg catcgcgaac
     8701 gagaaaccag tccgtgcgcc gcttgatcac ctcgaccact tcgccacgat cgacgacgtg
     8761 gaatttcgga tatccgcggc ccggtcccgt gtgcatctcg agatacgggt ccgcgaccga
     8821 tacggttcgg tagtcgccgt ccgcccaggc aagacccgca aagaaggttt ggcataagac
     8881 agcagccaca accgccaggg cactagttct gggggacgtc gaatgggttg ttgtagtatt
     8941 gcgcgccgac atccagccat tccgctatta atcgtttctc tgcaggggac aggtacccgt
     9001 cgtggctttg accggcatcg aagcggtcga agaattgttc cgatgcgttc gcccctgcca
     9061 cgttcatcga gggaccgacg ggtatggttg cgaagagcgg atcgcccgtg tccgggtcga
     9121 tgccgacctg gaccagcacg tcctgcaacg ctcccatgcc gccttcctgt gcgtcgtccg
     9181 gaaacaggag ttcccggtac gagttgaaat gcaccggttc atcgggagac aggccgtccg
     9241 aaaggtcgag ctgtcctgcc ggaacgcgta cgcctccctg gtcgtcgatg ggagtgtggc
     9301 aattcgtgca gtcgttgctg agcgggaagc cgcttccatc cagtacgggg ttccccatgt
     9361 catcaagtac cggccgcggc gcagcccaca gcggatgtat atgcgtttcg taattgatga
     9421 cgatgcggca gttcgcgctc cactgctgca tgcaatcgag gcttgtcggt accggcgtcg
     9481 tcaggtcggc atacgagtag tcgaagcttg catctttcgg gcgccccgct gccacttcat
     9541 cggtccatac atcctcgtag accaggtcaa cggacggctc gagcgatgaa cagttcgatg
     9601 cgccgcaggt tatgcgtgcc cttacctccg ccatcgtctc cccgacttcg ccgacgaaca
     9661 gcgcagggtc ggtgttcgga aacgtggtgc cggcagtcgg cgccccggcc cagacgctct
     9721 cgaaggcatc actccggccg tgcgaaacac cgctttgcgg catgtggcag ccgttgcatt
     9781 cgagcagttg cccgggtcgc acctgcagcc agttctggtg ccgtcctgaa atccggcgtc
     9841 cgtcggcgtc cagcacgctg acggccaggg cgacgttcgc cggcaccttc atcattaccg
     9901 aaccgtcggg ctcgattggg gcgtatccga cgatttcctt catgccctgc tgcgtgcttc
     9961 gtccgaaagc agtgccgtcg aggtcgacta tgtcctcatc ggggatagat accgctttca
    10021 cgacgcgaag aaagcgcgca gggcgctggt ccgccatggt ctgcgccggg tccgccaggg
    10081 cctcgatgtt cgcggtcgcg ccgccgtcga tgtcgtagac gctgcgtatg ctgatgacac
    10141 ccgcgccttc cccgaccagg tccggatcgg gagtgaaagt gttttcgttg tcgagaatca
    10201 ccgggggcgc tgccctgggg tcggccgaaa cggcctcggt gaatatgaag ccctcctccg
    10261 gtggcacgac cggcagctgc gtgttatccc ggggatcgta gatccagatt ccgtagatcg
    10321 ggggtgcggc ctcggcagcc gggtcgtcga gccggtctgc cgtgcatggc aggttgagca
    10381 tctcttccgt cacgcggcac tggttccagc tcgtgagaag ccgcccggtc ccatcctgga
    10441 tcgggaatac ggagcggaat ctcccgccgc tggacaatgc attcggcaac gtcgaaacct
    10501 cgagcaccgt cgcgggctcc tgcgcaggtc ccgtgagcgc cgcgttatcc ttgttcggtt
    10561 gtgtgatctc caggtacgca ggtgtatcga tcaccatgat gtcgccgccg gcgtcggtgt
    10621 cggagtacgg ccgtaccagc gccatgatcc gcccgtcttc gagttccctc ggctgcatga
    10681 actgcacggt cgcgccgtcc gtgccactgt cgtggctgtt ttgcccgtaa agaagctcga
    10741 gcgccgatcc gtcggggttc atccggtaca ggttcaccga gtcgtttacc cccatgtgat
    10801 cccagcggct gaacacgatc tgtccgttcg acatgaccga tggatcgaaa tcgtgactct
    10861 ggttgtacga cacctgctcg atgttgctgc cgtcggcgtc catgacgtgc agtacgaatg
    10921 ccatctcgtt cgcatcttca tccagcgccg ggaactgtgg ctttccctcg tccagcagaa
    10981 cggcagagga acgcaactgc cgcgtcgagg agaaaatgac gcggccatcc ggcaggaaat
    11041 gaggggccac gtcgtgaccg atctctgccg taaggtccga cgctatgacg cggcgcagtt
    11101 cgcc
//
LOCUS       DASUOM010000479        10178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1628701,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000479 DASUOM010000000
VERSION     DASUOM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628701"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..840)
                     /locus_tag="VFY27_09955"
     CDS             complement(40..840)
                     /locus_tag="VFY27_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5765881.1"
                     /translation="MRRHRSVFTLSMLAFGYAFLYIPLVSVIIYSFNDSRLATVWGGF
                     STRWYGELLRNEQVLDATFLSLRIAVVTATFATVLGTMAGMALARFGRFRGRTLFSGM
                     ITSPLVMPEVITGLSLLLLFVSLQQLIGWPAQRGFSTITIAHTTFALAYVTVIIQSRM
                     AAMDVSLEEAAMDLGGRPLRVVFDITLPLIAPAMVAGWLLAFTLSLDDLVIASFVSGP
                     GGSTLPMVIFSKIRLGVTPDINALATLIIVVVTVGVLIAWWAGQRSRP"
     gene            complement(837..1724)
                     /locus_tag="VFY27_09960"
     CDS             complement(837..1724)
                     /locus_tag="VFY27_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5765882.1"
                     /translation="MKAGPDMLWQRVATALPYLWLLVFFLAPFAIILKISFADPVIAQ
                     PPFTPLLDDTGAPQVTIDNYVFLLTDKLYIVTYLKSVFMAAVATLLCLIVGFPMAYGI
                     ARAERSVRNILLLLIILPFWISFLLRVYAWMGLLNNYGVINNLLLWMGVIERPLQLIY
                     TDMAIYIGLVYSYLPFMVLPLYATLERMEPDLVEASQDLGASPSQAFRDITWPLARPG
                     VIAGCLLVFIPAMGEYVIPYLLGGPESLMIGRVLFDEFFVNRDWPLASSVAIVLLTLL
                     VVPIVFLQRHRRDEVEEAG"
     gene            complement(1735..2859)
                     /locus_tag="VFY27_09965"
     CDS             complement(1735..2859)
                     /locus_tag="VFY27_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5765883.1"
                     /translation="MTAAAQNDLQQGAPVIRIRDVSKHFGDFVAVDSVNLDIHRGEIF
                     SILGASGCGKTTLLRMIAGFEVPTAGRIFIDGVDLTDQPPYRRPVNMMFQSYAIFPHM
                     TVEQNVSYGLQKERVPKNEIRSRVAEMLSLVQLEQFAARKPDQLSGGQRQRVALARAL
                     VKRPKVLLLDEPLAALDKKLREHTQFELMGIQDRLGITFVVVTHDQEEAMILSSRIAV
                     MNNGRLDQVGTPKQVYEYPNSRFVADFIGTINLFEGTVENVSEGAITVRCESAGAAMR
                     ALTDTPPAVGSKICLAIRPEKIFISQEEPESEWNTSLRGVVDDLGYFGNLSLYRIRLE
                     NGKIVQVSAQNRRRSAERFLEWDDQVFISWRAQSVVVLGS"
     gene            complement(2885..4012)
                     /locus_tag="VFY27_09970"
     CDS             complement(2885..4012)
                     /locus_tag="VFY27_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5765884.1"
                     /translation="MKLTLKPLLRLLVLAVVPMLVVSCTADSAPETAPGARVVNIYNW
                     SDYIAPDTIRNFEKEYGIKVNYDVYDSSEVVDAKLLAGSSGYDVVFHSDQFSVRLAPL
                     GVYQELDYSRFENLHHLDPEILGKTKLYENLEGYTLPYMWGSTGYAYNEEMVRRRLPT
                     AKMDSAAVLFDPAIVSKLADCGVSLLDSPTDVFPMVLSYLGRDPNAIDPESVAAAEAQ
                     LQLVRPYIRYFSSTKMISDLPNREVCVAMSWSGDYAQASARAEEAGIDIDLRYTMPKE
                     GSALWVDSMLIPTDAPHVDNAYLFMDYMLRPEVIASISNYIFYANANKSARPLLDAAV
                     VGDPGIYPDEESWALLFPVLPAEPKQERLRTRAWARVKSGI"
     gene            complement(4105..5265)
                     /locus_tag="VFY27_09975"
     CDS             complement(4105..5265)
                     /locus_tag="VFY27_09975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5765885.1"
                     /translation="MKPELDRAALEWQQKAREFAVSMLQPHEVEAEMNGGELPPDIRK
                     KHKATAIELGFSSMDVPKSLGGLELRIVEQVAVWEQLGRVTNALAWCFAEPQRWMFEA
                     CSELQVEKYILPLMRGEKHDCYAITEAGPGSDVAALEATARRSGGGYILNGEKWFVTS
                     ANLAHYFWFQAVLPEEQEDALFFVDQGQDGIEVIASPAFSHTYAAHHPTYRFSDVEVP
                     AENRVGRSGDGMAYTKSWFRRERLMIAARCCGAAARLIEEASEFAKGRTSGGIPLSER
                     QAIQFMLADSVTELWAARLMTFEAAAAHDRGEDVKSLHNRCSVAKLYASEMANRVADR
                     AVQIFGGRGYMRENVAERFFRELRVDRIWEGTSEIQRLIIARGLLKRGLEMM"
     gene            complement(5297..6049)
                     /locus_tag="VFY27_09980"
     CDS             complement(5297..6049)
                     /locus_tag="VFY27_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765886.1"
                     /translation="MAQPLQEARKEPASIVSPRVGLKRSSMITDEENTMAVAAVRTAA
                     KGAKSSRRTATREERRQQLIAATIKCISKKGIGSTTLGDVAQEAGLSQGIVNLHFASK
                     ENLFNETLQFLAEDYKAQFDGTLEKAGAEAAEKLLALMEMDLKPSVCDRQKLAVWFAF
                     WGEVKSVPTYQKICEAYDRGYDDAINALCQSIIDDGKYTNITAQTVTDALSSMTDGLW
                     LSYLVSPKGFDRDVAMDAVYSYLRAVFPEHYE"
     gene            6259..7530
                     /locus_tag="VFY27_09985"
     CDS             6259..7530
                     /locus_tag="VFY27_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5765887.1"
                     /translation="MSSNFRYWGDDKTIYAKRGKGGRLWDIDGNEYIDYRLAYGPAIL
                     GYADPRVDEAAREGMEIGGVFALATELEYEVASRISKMVPAAELVRFSNSGTEAVMAA
                     MRIARAHTGKDAHIVVEGGYHGVFNEVLWYADVEEWTPGKGEPVIRPFGEGIPAVLKP
                     LFHTVPLNDANAVEDVLRRHHQDIGAFLIEPIMGNCCGLNATREYLHDVRSLCDRYDV
                     VLIIDEVKTGFRVAKGGVQELLGVKADLCTFAKAMANGYPISVVAGREDLMRRVGDGV
                     VHGGTFTGHSVSLSAANRTLQILDETDALADIERYGKALQAGLKRILSARGIPHCFAG
                     HPSMTGLFLDDHAPKDYRDWINTDYEFYDALAPELHELGVLVEPDSREPWFICEAHDV
                     KCLEETLDKFERAVDITLKKLPSDSSRVHTA"
     gene            7617..9233
                     /locus_tag="VFY27_09990"
     CDS             7617..9233
                     /locus_tag="VFY27_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5765888.1"
                     /translation="MKTYDAIVVGAGHNGLTNAAFLAKAGLNVLCVEKNEYIGGATVS
                     RNLYKDWIYSNCSYVCSLLRPEIFRALELHRHGLQITPYGGGVTFMQDGGYFGTYWDA
                     EVAHREYSRFSKHDADAYERYSADVMRQTRFIRDSLLRTPPDPTSFRPRDLKELAHLG
                     RRFYDMGEDRMLETIRFWTMSVAEYLDEYFETDVIKTSLSGSGIIGTALGIHSPGTAY
                     VLLHHYMGEVDGNVGSWGYARGGMGAVSKAIAGAFQSYGGELRTDAGVEQIVVRNGSV
                     KGVALTNGEEIDAEIVVSGMDVKRTFLKCMDEKDLPPEFYRRVKNFKIRGSSGKLNIA
                     LDGMPTFPALPKNNPLLDGDMHFIDSMERMERAYDDWKEGTWSKDPYVDMVIPTVTDP
                     TMAPPGKHFMSCFIQYCPYKVEGRDWTQEEREAFARTVIDQIGNYSTDFKDLILHVEI
                     RTPADIEREAGLSEGNIFHGELTMDQLLFNRPVPGYAQYRGPVGGLYMCGSSAHPGGG
                     VMAAPGANAAREILLDLKRDNTVPGNFGDD"
     gene            9226..>10178
                     /locus_tag="VFY27_09995"
     CDS             9226..>10178
                     /locus_tag="VFY27_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5765889.1"
                     /translation="MTERYDAIVIGAGHNGLVCAALLAKAGKSVLVLEADASVGGAAR
                     TREFAGGYSVSSCAHLLYQLQPDVIKDLGLKIDLAARSIGTIALAAHGKHVRYSGGSV
                     EGVSNADRENLREFHRRMARFADLLQTYINKPPPRLANGGSRDMTTLARLGFDLRRLG
                     KTEMREFLRLIGMNIFDEVEERFESPLLRGALSLDAVLGTHLGPRSPNTILTYLYRLC
                     GSHGRLSAPAGGMGAVAEALAQAARDRGAVIRTGAPVKRVIVENGRAAGVETENGETF
                     SSWTVVSNADPKATILSLVGARHVETGFVRRVHNIRMRGNAA"
BASE COUNT         2043 a         3136 c         3077 g         1922 t
ORIGIN      
        1 agggcacggc gcggcgacgg caggaggcgt ggccagcgct cacggccgtg acctctgccc
       61 ggcccaccac gcaatcagta cgcccaccgt aaccacgacg atgatcaagg tcgccagcgc
      121 attgatatcc ggggtaaccc ccagcctgat cttcgagaaa atcaccatcg gcaaggtgct
      181 cccccccggc cccgacacga aacttgcgat aacgagatcg tcgagcgaaa gggtaaacgc
      241 cagcagccac ccggcaacca ttgccggtgc gatgagcggc aaggttatgt cgaagacgac
      301 gcgcaggggc cggcccccca agtccatcgc ggcctcttcg agcgacacgt ccattgcggc
      361 catgcgtgac tggatgatga cggtgacgta ggccagggcg aacgtggtgt gcgcgatggt
      421 gatcgtggaa aagccacgtt gcgccggcca gccgatgagc tgctggagac tgacgaacag
      481 caacagcagt gagaggccgg tgatgacctc cggcatgacc agcggcgatg taatcatgcc
      541 ggagaaaagg gtacggccgc gaaagcggcc gaagcgggcc aatgccatgc ccgccatggt
      601 gccgagaacg gtggcgaacg tggccgttac gactgctatt cgcaggctca ggaaggtcgc
      661 atccagcacc tgctcgttcc ggagaagctc gccgtaccag cgggtggaga aaccgcccca
      721 gacggtggcg agccgcgagt cgttgaacga gtagatgatg actgaaacca gcgggatgta
      781 gagaaacgcg tagccgaacg cgagcatcga taaggtgaag acggagcggt gccgcctcat
      841 cccgcttcct ccacttcatc gcggcggtgc cgctgcagga agacgattgg cactaccagc
      901 aacgtcagca gcacgatggc gacggacgat gcaagcggcc agtcgcggtt gacgaagaac
      961 tcgtcgaaca gcacacgacc tatcatcagc gattcgggtc caccgaggag gtaaggaata
     1021 acgtattcac ccatcgccgg gatgaacacc agcaggcacc ctgcaatgac gcccggccgc
     1081 gccagtggcc acgtgatatc gcgaaacgcc tgtgacggag aagcgccgag gtcctgcgat
     1141 gcttccacaa gatccggctc catacgctcc agcgtcgcat aaagcggcag gaccatgaat
     1201 ggcaggtagg agtagacgag gccgatgtag atggccatgt cggtgtagat gagttgcagc
     1261 ggccgctcga tgacgcccat ccagagcagc aggttattga tcacgccgta gttgttgagc
     1321 aggcccatcc acgcgtagac ccgtaacagg aacgatatcc agaacggcag gatgatgagg
     1381 agcagcagga tgttgcgcac ggagcgctcg gcccgggcaa tgccataggc catcgggaat
     1441 ccgacaatga gacacaggag cgtcgcgacg gcggccatga agacggactt caggtacgtg
     1501 acgatgtaca gcttgtccgt cagcaggaaa acatagttgt cgatcgttac ctgcggtgca
     1561 ccggtgtcgt ccagcagcgg cgtgaacgga ggctgcgcga tgaccggatc ggcaaaactg
     1621 attttcagga tgatcgcgaa cggtgcgaga aaaaaaacca ggagccagag gtacggcagc
     1681 gcggtcgcga cgcgctgcca gagcatatcc ggaccggctt tcacgtgtac gccactagct
     1741 gcctagtacc acgacgcttt gcgctctcca ggaaatgaat acctggtcat cccattccag
     1801 gaaacgctcg gcggagcggc ggcggttctg tgcactcacc tgtacgattt tgccgttctc
     1861 gaggcgaatc ctgtacagcg acaggttgcc gaaatagccg aggtcatcta cgactccgcg
     1921 caggctggtg ttccactcgc tctccggttc ttcctggctg atgaaaatct tctccgggcg
     1981 aatggcgagg caaatcttcg aacctacggc cggcggtgtg tccgtcaatg cccgcatggc
     2041 cgcgccggcc gactcgcagc gcaccgtgat ggctccttcc gacacgttct cgaccgtccc
     2101 ctcgaacagg ttgatggtgc cgatgaagtc cgcgacgaag cggctgttcg ggtattcgta
     2161 gacctgcttc ggcgtgccga cctggtcgag gcgcccgttg ttcatcaccg cgattcggct
     2221 cgacaggatc attgcttctt cctggtcgtg cgtgaccacg acgaacgtaa tgcctaagcg
     2281 atcctggata cccatcagtt cgaactgcgt gtgctcgcgc agtttcttgt ccagtgccgc
     2341 gagcggctcg tccagcagca acacttttgg ccgtttcacc agcgcccgcg caagcgccac
     2401 tctctgccgc tgccccccgg acagctggtc aggcttccgg gcagcgaact gctccagctg
     2461 caccagactg agcatctccg ccacacgcga gcgaatctcg ttcttcggca cccgttcctt
     2521 ctgcaggccg taagacacgt tctgttcgac ggtcatgtgc gggaaaatcg cataggactg
     2581 gaacatcatg tttaccgggc gccggtaggg cggctggtcg gtcaggtcga caccgtcgat
     2641 gaagatgcgg cccgcagtcg gcacctcgaa acccgcaatc attcgcagca aagtcgtttt
     2701 gccacacccg gacgcgccga gaatcgagaa tatctccccg cgatggatgt caaggttgac
     2761 cgaatcgacc gcgacgaagt cgccaaagtg cttgctcaca tccctgatcc ggatgacggg
     2821 agcaccttgc tgcaggtcgt tctgcgccgc agccgtcatt taccgtttgg ccttcgcaac
     2881 gacgtcagat gccgctcttg acgcgcgccc aggcgcgcgt acgcagacgc tcctgcttcg
     2941 gctcggcagg caacaccggg aacagcagtg cccacgattc ctcgtccgga taaatgcccg
     3001 gatccccgac caccgccgcg tccagcagcg gcctggcaga tttattggcg tttgcgtaaa
     3061 aaatgtaatt ggagatggag gcgatcactt ccgggcgcag catgtaatcc atgaacaggt
     3121 aggcgttgtc gacgtgcggc gcatcggtcg ggatcagcat gctgtcgacc cagagtgccg
     3181 agccctcttt cggcatcgtg tagcgcaggt ctatgtcgat acctgcctcc tcggcgcgtg
     3241 cgctggcttg cgcatagtcc ccggaccagc tcatcgcgac gcatacttcc ctgttcggca
     3301 ggtcgctgat catcttggtc gagctgaagt agcggatata cggccggacc agctgcagtt
     3361 gcgcctcggc cgcggcgacg ctctcgggat cgatggcgtt cggatcgcgg ccgaggtagg
     3421 acagcaccat cgggaacaca tcggtcgggc tgtcgagcag gctgacgccg cagtcggcaa
     3481 gtttcgacac gatcgccggg tcgaaaagca ctgcggcgct gtccatcttt gctgtcggca
     3541 accgccttct caccatctcc tcgttgtagg catatcccgt cgagccccac atgtacggca
     3601 aggtatagcc ttcgaggttt tcataaagct tcgtcttgcc gagaatttcc gggtccagat
     3661 ggtgcaggtt ttcgaacctc gaatagtcga gttcctggta gacaccgagc ggtgccaggc
     3721 ggacggagaa ctggtcgctg tgaaagacca cgtcgtaccc gctggatccc gccagcagct
     3781 tcgcgtcgac tacctcggag gaatcgtaaa cgtcgtagtt gaccttgatg ccgtattctt
     3841 tttcgaagtt gcggatggtg tccggcgcga tgtagtcgga ccagttatag atatttacga
     3901 cgcgagcgcc cggagctgtt tcaggtgcgc tgtccgccgt gcaggacaca accagcatcg
     3961 gcacgaccgc cagcaccagg aggcgcaata gaggctttaa ggtcagtttc atccgtcttc
     4021 gatttcacca cttgcccgga aacaagacaa tcgtcacccg aactgctgct cgatgaccag
     4081 cgaggcccgg ccatgtcgag caagttacat catttcaagg ccgcgcttca gcaagccgcg
     4141 ggcgatgatc aggcgctgga tttcgctggt gccttcccag atgcggtcca cccggagttc
     4201 acggaaaaat cgctccgcaa cgttttcgcg catgtaaccg cggccgccga agatctgcac
     4261 ggcacggtcc gctacccggt tcgccatctc cgacgcgtac agcttggcca cggaacaacg
     4321 attatgcagg cttttcacgt cctcaccccg atcgtgcgcg gcggccgcct caaaggtcat
     4381 cagccgggcc gcccataact ccgtgacgct gtccgcaagc atgaactgga tagcctgccg
     4441 ttcgcttaag gggatcccgc cggacgtgcg ccctttcgcg aattcgctcg cctcctcgat
     4501 gagcctcgcc gcggcgccgc agcaacgggc cgcgatcatc agccgttcgc ggcgaaacca
     4561 gcttttcgtg taagccatgc cgtcgccgga gcggccgacc cggttttccg caggaacctc
     4621 cacatcggaa aaacggtacg tcgggtgatg tgccgcatac gtatggctga atgcaggcga
     4681 tgcgatgacc tcgatcccgt cctggccctg gtcgacgaag aaaagcgcgt cctcctgctc
     4741 ctccggcagc accgcctgga accagaagta gtgggccagg ttcgcactgg tgacgaacca
     4801 cttctcgccg ttcaggatgt agccgccgcc cgaccttcgg gcagtcgctt ccagcgcggc
     4861 gacatcggag cccggacccg cctccgtgat tgcataacaa tcgtgcttct cgccgcgcat
     4921 aaggggaagt atgtacttct caacctgcag ttcgctgcag gcctcgaaca tccagcgttg
     4981 cggctccgcg aaacaccagg caagcgcgtt ggtaaccctg ccgagctgct cccacaccgc
     5041 gacctgctcc acgattcgaa gctcgagccc ccccagggac ttcggcacgt ccatcgaact
     5101 gaagcccagt tctatcgcgg tggccttgtg tttcttgcga atgtccggcg gcagttcgcc
     5161 gccgttcatt tcggcttcga cttcgtgcgg ctgcagcatg cttacggcga actcgcgcgc
     5221 cttctgctgc cattcgagcg cggcccggtc cagttccggt ttcaagttcc attcctcccg
     5281 accagtacgc ccgtcatcat tcgtaatgct ccgggaaaac agcccgaagg tagctgtata
     5341 ccgcgtccat cgccacgtcg cgatcgaagc ccttcggact gacgaggtag gacagccaca
     5401 ggccatccgt catggatgac aaagcgtccg ttacggtctg cgcagtgatg ttcgtatact
     5461 tgccgtcgtc gatgatcgac tggcagagcg cgttgatcgc gtcatcgtaa ccgcggtcat
     5521 aggcttcgca gatcttctgg taggtcggca ccgacttcac ttcgccccag aacgcaaacc
     5581 agaccgcgag tttctgtcgg tcgcacacag aaggcttgag gtccatttcc atcagcgcga
     5641 ggagtttttc cgccgcttcg gctccggcct tctccagcgt accgtcgaac tgcgccttgt
     5701 agtcctccgc gaggaactgc agcgtttcgt tgaacaggtt ctccttgctg gcaaagtgca
     5761 ggttcacgat gccttgcgag agccccgctt cctgcgccac gtcgcccagc gtcgtgctgc
     5821 cgattccttt cttggagata catttgatcg tggcggcgat cagctgctgt cgacgctcct
     5881 cacgggtagc ggtccgacgg ctgcttttcg cgcctttcgc tgcggttctg actgcggcaa
     5941 ctgccatcgt gttctcctcg tcggtaatca tgctgctacg cttcaggccg accctgggtg
     6001 aaactatcga cgccggttcc ttccgcgcct cctgcagcgg ctgcgccatg gagataatcc
     6061 tgcgcaaatc cgctcccttt tccccctcgg cgacccgcat cctgccacaa aatgaatgag
     6121 tattcattca tatttctgga cttcggacac cgtccgtgag aggctgggct ccaggaaaag
     6181 tcacatggag gcaatatgag gtgtctgcac aaatctaatc aacaattccg gaaagccaac
     6241 caaaaactcc cgctgggcgt gtcatcgaac ttccgctact ggggcgacga caagaccatc
     6301 tatgccaagc gcggcaaggg cggccggctc tgggacatag atggtaacga atacatcgat
     6361 tatcgcctgg cctacgggcc ggctattctg ggctatgccg acccccgcgt cgatgaggct
     6421 gcccgcgaag gcatggaaat aggcggggta ttcgcgctcg ccaccgaact cgaatacgaa
     6481 gtcgccagtc gcatcagcaa aatggtgccc gcagccgaac tggtgcgctt ctccaattca
     6541 ggcaccgagg ccgtcatggc tgcgatgcgc atcgcgcggg cacataccgg gaaggacgct
     6601 catatcgtgg tcgaaggcgg ttaccatgga gtcttcaatg aagtgctctg gtacgccgac
     6661 gtcgaagaat ggacgccggg aaagggcgag ccggtcattc gcccctttgg cgagggcatt
     6721 ccggctgttt tgaagcccct gttccacacc gtcccgctga acgatgcgaa cgcggtggaa
     6781 gacgtgctcc gccggcacca ccaggacatc ggcgcattcc tgatcgaacc gatcatgggc
     6841 aactgctgcg gattgaatgc cactcgcgag tacctgcacg acgtccgttc cctttgcgac
     6901 aggtatgacg tggtccttat catcgatgag gtcaagacag gatttcgcgt ggccaaaggc
     6961 ggcgtccagg aactgctcgg cgtgaaggcg gacctttgca cctttgccaa ggcaatggca
     7021 aatggctacc cgatatcggt ggtggccggg cgggaagacc tgatgcgcag ggtcggcgac
     7081 ggcgtcgttc atggcggcac cttcaccggg cattcggtgt cgctgtcagc ggccaacagg
     7141 acgttgcaga tcctggacga aacggacgcg ctcgccgaca tcgaacgata cggcaaggca
     7201 ttgcaggcag gactgaagcg catcctgtcg gcacgcggca ttccgcactg ttttgccggg
     7261 cacccgtcga tgacgggatt gttcctggac gatcacgctc ctaaagacta ccgcgactgg
     7321 ataaacaccg attacgagtt ttacgatgcg ctggcgcccg agctgcacga actgggtgtc
     7381 ctggtagagc cggactcacg tgaaccgtgg ttcatctgcg aggcgcatga tgtgaaatgt
     7441 ctggaggaaa ctctggacaa attcgagcgc gcggtggaca ttacattgaa gaagctgccc
     7501 tccgacagca gccgcgtcca cactgcgtag aagaaccaag aaactgcagc ggccctcaag
     7561 ggccgctgcg gttgtttcgt tgcctgttgc aggcactttc gagcatcctt atttcaatga
     7621 aaacctatga tgccatcgtc gtcggcgccg gtcacaacgg actgacaaac gccgcttttc
     7681 tcgccaaagc aggcctgaac gttctctgcg tcgaaaaaaa cgaatatatc ggcggcgcca
     7741 cggtcagccg caacctgtac aaggactgga tctattcgaa ctgttcctac gtctgttcgc
     7801 tgctgcgccc ggagatattc agggcgctgg aacttcaccg gcatggcctg cagataaccc
     7861 cgtacggggg cggtgtgacg ttcatgcagg acggtggcta cttcggcacc tactgggatg
     7921 cggaagtggc gcatcgtgaa tattcgcgct tctcgaaaca cgacgcagac gcctatgagc
     7981 gctactcggc ggacgtcatg cgccagacgc gcttcatccg cgactcgctg ctgcgtaccc
     8041 cgccggaccc gacgtcgttc agaccccgcg acctcaagga gctggcgcac ctgggccggc
     8101 gcttttacga catgggcgag gacaggatgc tggagacgat tcgcttctgg accatgagcg
     8161 tcgcggagta cctggacgaa tattttgaaa ccgacgtcat caagacttcg ctgtccggca
     8221 gcggcatcat cggcacggca ctcggcattc attccccggg tactgcctac gtgctgctgc
     8281 accactacat gggtgaagtc gacggcaacg tcggatcctg gggttacgcg cgcggaggca
     8341 tgggagcggt gtcgaaagcg atcgccggcg ctttccagtc ttacggaggc gaactgagga
     8401 ccgacgccgg ggtcgagcag atcgtggtgc gaaacggttc cgtcaaaggg gttgcattga
     8461 cgaacggcga ggagatcgat gcggagatcg tcgtgtccgg gatggacgta aagcgcacgt
     8521 tcctcaagtg catggacgag aaagacctgc cgcccgagtt ctaccggcgc gtcaagaact
     8581 tcaagatccg cggctcttcc ggaaagctga acattgccct ggacgggatg cccacgttcc
     8641 ccgccctgcc gaagaacaac ccgctgctcg acggcgacat gcatttcatc gattccatgg
     8701 aacgcatgga gcgtgcctac gacgactgga aggaaggtac gtggtcgaag gacccgtacg
     8761 tagacatggt catcccgacg gttaccgacc cgacgatggc gcctcccgga aagcacttca
     8821 tgagctgctt catccagtac tgcccgtaca aggtcgaagg tcgcgactgg acgcaggaag
     8881 agcgtgaagc gttcgccagg acggtgatcg accagatcgg taactacagc acggatttca
     8941 aggacctgat cctgcacgtc gagatacgga cgccggcgga tatcgagcgc gaagcgggtt
     9001 tgtccgaggg caacatcttt cacggcgagc tgacaatgga ccagcttctg ttcaaccggc
     9061 ctgtgccggg ctacgcacag taccgcggcc ccgtgggggg gctgtacatg tgcggctcga
     9121 gcgcccatcc cgggggcggc gtcatggcgg ctcccggcgc caacgcggcc agggagatcc
     9181 tgctggacct caaacgtgat aacaccgtcc ccgggaattt cggcgatgac tgagcgctac
     9241 gatgccattg tgatcggcgc gggccataac ggcctggtat gcgccgcgtt gcttgccaaa
     9301 gccggcaaat cggtcctggt gctcgaggcg gacgcgagtg tcggtggtgc cgcgcgcaca
     9361 cgcgaattcg ccggcggcta ttcggtgtct tcctgcgcgc acctcctcta ccagctgcag
     9421 cccgacgtca tcaaggacct cggcctgaag atagatctcg ccgccaggag tatcggcacg
     9481 atcgccctgg ctgcccatgg caagcatgtt cgctacagcg gcggaagcgt cgaaggagtg
     9541 agcaacgccg accgcgagaa cttgcgggaa ttccaccgcc gtatggcccg cttcgcggac
     9601 ctgctgcaga cgtatatcaa caagccgccg ccgcgacttg caaacggcgg atcgcgggac
     9661 atgacaacct tggcgcgact cggcttcgac ttgcggcgcc tcggcaagac cgagatgcgt
     9721 gaattcctgc gcctcatcgg tatgaacatt ttcgacgagg tggaggagcg tttcgaaagc
     9781 ccgctgctgc gcggcgcgct gagtctcgat gcggtcctcg ggacgcatct tggtcctcgt
     9841 tctcccaata cgatactgac ctatctttat cgcctgtgcg gcagccacgg cagactatcc
     9901 gcgccggcgg gcggcatggg tgccgttgca gaggcgcttg cgcaagccgc acgcgaccgc
     9961 ggcgcggtga tacgcacagg ggctccggtc aagcgggtaa tcgtcgagaa cggccgcgcg
    10021 gcgggcgtcg agacggagaa cggggagacg ttctccagct ggaccgtcgt ttcgaacgcg
    10081 gaccccaaag cgaccatcct gtctctcgtt ggcgcgcgtc acgtcgagac cggcttcgtg
    10141 cgccgcgtgc acaacatccg catgaggggc aacgcggc
//
LOCUS       DASUOM010000480         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1745736,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000480 DASUOM010000000
VERSION     DASUOM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745736"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFY27_10000"
     CDS             complement(<1..269)
                     /locus_tag="VFY27_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765890.1"
                     /translation="MRRRRRSAEVFSLSFLDCICCGFGAIILLLVLSKIYEPVIMEKS
                     EQDLRELIAMLQEELFEIQGETTVLNRELVSAQTITTDTKMTLAEL"
     gene            complement(292..1389)
                     /locus_tag="VFY27_10005"
     CDS             complement(292..1389)
                     /locus_tag="VFY27_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765891.1"
                     /translation="MARKRRDIEVFSMSFLDCMSCGFGAVILFFMIINAQTTRDVDAP
                     PERLMAETNRLEYEILDKRKNLVLARNTMEQLEDERAEAENEIAQITALIEKIKIELA
                     KHDNETLAKIERVEKLQSDIESLEEQRKRLLAMEKEKLEEGTKVRSFTGDGDRQYLTG
                     LKVGGERILVLVDASASMLDRRIINIIRRRNMPEAERLRSAKWRQAVASVDWLSAQFD
                     PESKFQIYTYNTEAKPVLKGSEGVWLEVGDGEQLSEAIRILRRTVPQNGTNMMSAYDV
                     INQLNPPPDNVIVLADGLPTMNAPQTERGMVTGQERLRYHYEAEALVPSGIPVNILLY
                     PMEGDYNAAIAYWLLAYRTGGSFMSVSEDWP"
     gene            complement(1379..>2226)
                     /locus_tag="VFY27_10010"
     CDS             complement(1379..>2226)
                     /locus_tag="VFY27_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5765892.1"
                     /translation="CGIYPLYDIWLNTLAEAFVQQSRFDPLHTAETEQMLLDNLATWL
                     ALSAQRDRVELELTYDGITHKAEVESLALVGAAAPVYQPIASKLRALYRAEETPAIQV
                     TDRIACLPGLPDMLKARVGGELFVLEPGATARGALARIRDSTVRGSAISLRRHLPWDQ
                     SGLEVERDETARVHAGIPTHLLYGSKAYAIAASPPLVVGSQAMDGERVIGLGTDMPGI
                     SRRHCSISLRNGQCVLEDHSRYGTFLNGHRIDGSTVLQVGDTVRVGSPGYEFRMITTD
                     ETYGA"
BASE COUNT          370 a          712 c          671 g          473 t
ORIGIN      
        1 agctccgcaa gcgtcatctt cgtgtcggtc gtaatggttt gcgcggagac gagttcccgg
       61 ttgagcacgg tggtctcgcc ctggatctcg aacagttcct cctgcaacat cgcaatgagt
      121 tcgcgcaggt cctgttcgct cttctccatg atcaccggct catagatctt gctgagcacg
      181 agtagcagaa taatggctcc gaaaccgcag catatgcagt cgaggaaaga gagactgaac
      241 acctccgcgc ttcttctgcg acgtctcacg ccggtttcct cccgaccgcc gtcacggcca
      301 gtcctcgctc acactcatga acgagccccc ggttcgatac gcgaggagcc agtatgcgat
      361 agccgcgttg tagtcgccct ccatcgggta cagcaggata ttgacgggta tcccgctcgg
      421 taccagcgct tcggcctcgt agtggtagcg tagccgttcc tggcccgtca ccatgccgcg
      481 ttcggtttgc ggcgcattca tggtcggcag gccgtctgcc aggacgatca cattgtccgg
      541 cggcgggttc aactggttga tgacatcgta ggccgacatc atgttggtgc cgttttgcgg
      601 taccgtgcga cgcaggatgc gaatggcctc gctcagttgc tcgccgtctc ccacctccag
      661 ccagacgccc tcgctgcctt tcaataccgg tttcgcttcc gtgttgtacg tgtagatctg
      721 aaacttgctt tcgggatcga attgcgccga cagccagtcc acgctcgcga cagcctggcg
      781 ccacttcgcg gaacgaaggc gctccgcctc gggcatgttg cggcgccgga tgatgttgat
      841 gatgcgccgg tcgagcatgc tggcggacgc atccaccagc accaggatac gttcgccgcc
      901 caccttgagg ccggtcaggt actgccggtc accatcgccg gtgaaggatc gcaccttcgt
      961 tccttcttcc agcttctcct tctccatggc aagcagccgc tttcgctgct cttccagcga
     1021 ctcgatatcc gattgcagtt tctcaacccg ctcgattttc gccagcgttt cgttgtcgtg
     1081 cttcgccagc tcgatcttta tcttctcgat cagggccgtg atctgggcga tttcgttctc
     1141 ggcctcggcg cgttcgtcct cgagctgctc catcgtgttg cgcgccagca cgaggttctt
     1201 gcgtttgtcg agaatctcgt attccagccg gttcgtctct gccatcagcc gctcgggcgg
     1261 cgcatcgacg tcccgcgtcg tctgcgcgtt gatgatcatg aaaaacagga tgactgcccc
     1321 gaaaccgcaa ctcatgcagt cgaggaacga catgctgaag acttcgatgt cacggcgttt
     1381 acgcgccata cgtctcgtcc gttgtgatca tcctgaactc atagcccggc gagccgactc
     1441 tcacggtgtc gccgacctgc aacactgtag acccgtcgat ccggtgaccg ttcaaaaacg
     1501 tgccatagcg gctgtgatcc tccaacacgc actggccgtt acgcaggctg atcgaacaat
     1561 ggcggcgcga gatgcccggc atgtcggtgc ccagtccgat gacacgttcc ccgtccatgg
     1621 cctgggaccc cactacgagc ggcggcgacg cggcaatggc gtaagccttg ctgccgtaca
     1681 gcaggtgcgt cgggatcccc gcgtgcaccc gcgcggtttc gtcgcgctcg acctcgaggc
     1741 ctgactggtc ccaggggagg tgccgacgca ggctgatggc cgagccgcga acggtggaat
     1801 cgcggatgcg ggcaagagcg ccgcgggcgg tcgcgcccgg ctccagtacg aacagctcgc
     1861 ctcctacgcg tgccttgagc atgtccggca ggccaggcag gcaggcgatg cggtccgtca
     1921 cctggatggc gggcgtctct tccgcgcggt agagcgcgcg cagtttgctg gcaatcggct
     1981 ggtaaaccgg ggccgcggcg ccgacgagcg ccagtgactc cacttccgcc ttgtgggtga
     2041 ttccgtcata cgtcaattcc agttccaccc ggtcgcgctg cgccgagagc gcgagccagg
     2101 tggcgaggtt gtccagaagc atctgttcgg tttcggcggt gtgcagcggg tcgaaacgcg
     2161 actgctggac gaaggcctcc gcgagagtgt tgagccagat gtcgtacagg ggatagatgc
     2221 cgcagt
//
LOCUS       DASUOM010000481         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1020783,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000481 DASUOM010000000
VERSION     DASUOM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020783"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="rsgA"
                     /locus_tag="VFY27_10015"
     CDS             <1..506
                     /gene="rsgA"
                     /locus_tag="VFY27_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5765893.1"
                     /translation="AGYASIGYPTQRCSAKNNVNLDPLRLFLANRTAIIVGQSGVGKS
                     SLINVLVGNSARPVAPVSSSSGEGRHTTVNSVMLRLPSGGAVIDSPGVRDYAPAIDNP
                     ADVAQGFREIRDAAAHCKFSNCRHLQEPACNVKKQVNDGLVSERRYESYRRLLFSAER
                     LAERFTR"
     gene            complement(571..924)
                     /locus_tag="VFY27_10020"
     CDS             complement(571..924)
                     /locus_tag="VFY27_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX5765894.1"
                     /translation="MTSSPDCLFCKIVAGKINADIVHENDSVVAFRDINPQAPSHVLI
                     IPRNHVATINDLTESQASDVGQIFLAAKEVAKMEGLEERGYRVVMNCMEGAGQSVFHI
                     HLHLLGGRSLRWPPG"
     gene            complement(1004..1603)
                     /gene="recR"
                     /locus_tag="VFY27_10025"
     CDS             complement(1004..1603)
                     /gene="recR"
                     /locus_tag="VFY27_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5765895.1"
                     /translation="MSFSPLLVRLIEALRCMPGVGRKSAQRIAFHLLERDTEGASQLS
                     QALAAAVEGIGHCSRCRMFSEQELCDICSSPGRDETLLCVVESPADVMAVEDATGYKG
                     HYFVLMGHLSPLDGIGPEELGLQKLEERLVRENFHELIIATNPTVEGDATAHYLADMA
                     SRHAVPASRIAHGVPLGGELEYVDGGTLSHAFFGRRAVD"
     gene            complement(1717..2040)
                     /locus_tag="VFY27_10030"
     CDS             complement(1717..2040)
                     /locus_tag="VFY27_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5765896.1"
                     /translation="MKGGIGQLMKQAQQMQAEMQKAQEEMARMSVTGESGGGMVRITM
                     TCKHDVQSLDIDSSLLGDDKEMLEDLIVAAFNDALRRVDTTVKDRFSGMAAGLNLPPG
                     MKLPF"
     gene            complement(2062..>2981)
                     /locus_tag="VFY27_10035"
     CDS             complement(2062..>2981)
                     /locus_tag="VFY27_10035"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765897.1"
                     /translation="HVSAVLGFLADGDAPGLIKAIATIDEQFPDYARLLDDLTRLLQR
                     VAVYQAVGATDSDDDLDEAEIAGFASRIAAEDVQLFYQTALIGRRDLDLAPDPRSGVE
                     MTLLRMLTFRPQQNIDAAGPQASAAPPRPRSPTPAPGASRTGKLKIVEPPQQAKDDFA
                     EVQQPADWGKVIQVLGLGGASKLLAANCAWLRRDADTVYLGLDRRSESLLTRSRQQAI
                     ADALSAKFGQPLKVDISVAASSGESETPIQEQARRSDEKLVAARESLEADPNVKALRD
                     MFGAELNPDSIEPIDKPGGRPIDRERVGN"
BASE COUNT          526 a          967 c          882 g          606 t
ORIGIN      
        1 tcgccggcta cgcaagcatt ggctatccca cgcagcggtg cagcgcgaaa aacaatgtca
       61 acctcgaccc gctgcgccta ttcctggcca accgcactgc aatcatcgtc ggccagtcgg
      121 gtgtcgggaa gtcgagcctc attaacgtcc tggtcggcaa cagcgctcgt ccggtagccc
      181 cggtgtcctc atccagcggc gaaggccgcc acaccaccgt caactcggta atgctgaggc
      241 taccgtctgg cggcgccgtc atagactcac ctggcgtgcg ggactatgca ccggcgatcg
      301 ataaccccgc cgatgtggcg cagggcttcc gcgagatccg ggacgctgcg gcgcactgca
      361 agttcagcaa ctgccgccat cttcaggagc cggcttgcaa cgtgaagaag caggtcaacg
      421 acgggctcgt cagtgagaga cgctacgaaa gctatcgccg gctgctgttc agcgccgagc
      481 ggcttgcgga gcgtttcacc cgttagtgtc ctgaatcagt agttcattgc ctaagttgcg
      541 cgagcttccc tggttacgat tgcctggcgg ttatcctggt ggccaccgca gcgaacgtcc
      601 gccgagcagg tgcaggtgga tgtgaaacac ggactgccct gccccttcca tgcagttcat
      661 caccacgcga tatccgcgct cctccaggcc ttccatcttt gccacctcct tcgcggccag
      721 gaatatctgg ccaacgtcgc tcgcctgact ttcggtaagg tcgttgatcg ttgcgacgtg
      781 gttgcgcgga atgatcagga cgtggctcgg cgcctgcggg ttgatgtcgc gaaacgcaac
      841 cacgctgtca ttctcatgaa cgatgtcggc gttaatcttg cccgccacga ttttgcagaa
      901 caggcagtca gggctgcttg tcatggtgct ttacctcgct tcggttacga gtttgcattt
      961 acaggcgcgg atcgaaccgc ttgttgcagg ccacgcggcc gcatcagtca acggcgcgcc
     1021 gcccgaagaa cgcatgcgac agggttccgc catccacgta ttcgagttcg ccaccgagcg
     1081 gcacaccgtg cgcgatcctg cttgcaggca cagcgtgcct gctggccatg tcggcgaggt
     1141 agtgagccgt agcatcgccc tcaacggtgg gattggtcgc aatgatgagc tcgtggaaat
     1201 tttcgcgaac tagtcgctcc tccagtttct gcagtcccag ttcctccggc ccgatgccgt
     1261 ccagggggga caggtgtccc atcagcacga agtagtgccc tttgtaaccc gtcgcatcct
     1321 ctaccgccat gacgtccgcg ggtgattcga cgacgcaaag aagtgtctcg tcccggccgg
     1381 gggagctgca gatgtcgcaa agctcctgtt ctgaaaacat ccggcagcga ctgcaatggc
     1441 cgataccttc tactgcggct gcgagagcct gcgacagttg cgacgcgccc tccgtatccc
     1501 gctccagcag gtggaaggcg atccgctgcg ccgatttgcg acctaccccc ggcatgcagc
     1561 gcaacgcctc gatcaggcga acgagcaacg gtgagaatga cacttaagaa attgccctgg
     1621 gcctatgtag gagcccgcca tgcgggcgag cgcgatggag attggcgaac tcgcccgcat
     1681 ggcaggctcc tatagctatc cgaaacatga ccatcgctag aacggcagct tcatgcccgg
     1741 aggcaagttg agcccggcgg ccataccgga aaaccggtcc ttcacggtcg tgtccaccct
     1801 gcgcaacgcg tcgttgaatg cggcgacgat caggtcttcg agcatttcct tatcgtcacc
     1861 cagcagcgac gagtctatgt ccagcgactg cacatcgtgc ttgcaggtca tcgtgatcct
     1921 caccatcccg ccgcccgact caccggtgac cgacatgcgc gccatctctt cctgcgcctt
     1981 ctgcatttcc gcctgcatct gctgggcctg tttcatcagc tgcccgatgc cgcctttcat
     2041 cgctctactc cccggggtga actagttacc gacgcgttcc ctgtcaatgg gtctgccgcc
     2101 aggtttgtca atgggctcga tcgaatccgg gttcagctcc gcgccgaaca tgtcccgaag
     2161 cgcctttacg tttgggtccg cctcgaggga ctcgcgcgcc gctaccagct tttcgtctga
     2221 acggcgggcc tgctcctgta ttggcgtctc gctttccccg gaggatgcag ccacgctgat
     2281 atccaccttc agcggctgcc cgaatttcgc ggaaagcgca tccgcaatgg cctgctgtcg
     2341 cgatcgtgtg agcagggact cagaacggcg atcaagcccc aggtaaaccg tatcggcgtc
     2401 gcggcgcagc caggcgcagt ttgccgcgag gagcttgctc gccccgccaa gacccagcac
     2461 ctggattacc tttccccagt ccgccggctg ttgcacctcg gcgaagtcgt cctttgcctg
     2521 ctgtggcggc tccactatct tgagtttgcc cgtccgggag gcaccgggtg ctggagttgg
     2581 tgaccgtggc cgcggtggcg ccgccgacgc ttgcggtcca gcagcgtcga tgttttgttg
     2641 cggcctgaag gtaagcatcc ggagcagtgt catctcgacc ccgctcctgg gatccggggc
     2701 gagatccagg tcccgccggc cgatcagcgc cgtctggtag aagagttgca cgtcctcggc
     2761 agcaatcctg gaggcaaacc ccgcaatttc ggcctcgtcg aggtcgtcgt cgctgtccgt
     2821 ggctccgaca gcctggtata ccgcgacacg ttgcaacagg cgggtaaggt cgtccagcag
     2881 ccgggcgtaa tccgggaact gttcatcaat ggttgcgatc gccttgatga gtccgggtgc
     2941 gtccccgtct gccaggaaac cgagaacggc gctcacgtgc t
//
LOCUS       DASUOM010000482         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_779585,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000482 DASUOM010000000
VERSION     DASUOM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779585"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFY27_10040"
     CDS             complement(<1..381)
                     /locus_tag="VFY27_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5765898.1"
                     /translation="MIELLLCSVLTILPDFLYRRFVQGKRIGREITLYSIWFELRYGI
                     TACIALTITLLTLILYYHPSSSHAISFYRTVPVLPEGSGRVDEVLVKVRDKVKAGQPI
                     FKLDSSEEKAALETARRRLAELDAQ"
     gene            complement(378..902)
                     /locus_tag="VFY27_10045"
     CDS             complement(378..902)
                     /locus_tag="VFY27_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765899.1"
                     /translation="MFITAPDESDTFLIACAIFLVLAVLGLGSLYFRLHSLPERLAHG
                     NTHKLQFEVVAVLALLALFTHNNALWVAALLLALVPIPDLYTPLAGMAASLAKMAGWR
                     RPRTDLPSAATPAENVRKTPPATGDARQSDLMETMEDRLNIETCSAGGDGDPAQGGSA
                     GKRQHAASKERQRS"
     gene            complement(1210..1596)
                     /locus_tag="VFY27_10050"
     CDS             complement(1210..1596)
                     /locus_tag="VFY27_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765900.1"
                     /translation="MQSGKLIYVVDDDVEYLRGIELLLSAHGLGTRTFTSAEALQADA
                     DVAEAECLILDIHLGGISGIELLYWLSGAGIGVPVVLVTANDTETTRKAAAAAGCGAF
                     LVKPFSAKTLLDAMNRATRPDDIRSS"
     gene            complement(1707..>2075)
                     /locus_tag="VFY27_10055"
     CDS             complement(1707..>2075)
                     /locus_tag="VFY27_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5765901.1"
                     /translation="EAEKAQREWLGSAQLSLQGLTPREREVFDYVVRGELNKQTAHAL
                     GITERTVKAHRQHICEKLHARSVIDLVSLAVRLGIVSGAPTVGVPQDAGTVVRSRIQR
                     EARLKGETLGTGEDCYRRDT"
BASE COUNT          429 a          640 c          599 g          407 t
ORIGIN      
        1 ctgggcgtcg agctcagcaa ggcgccggcg tgccgtctcg agagcggcct tctcctcaga
       61 gctgtcgagc ttgaatatgg gctggccggc tttgaccttg tcacgcacct taaccaggac
      121 ctcgtcgacg cggcccgacc cttcgggaag gaccggaaca gtgcgataga acgaaatcgc
      181 gtgcgacgac gaaggatgat aataaagaat gagcgtcaat agtgtgatcg tgagagcgat
      241 acacgccgtt atcccgtatc tcagctcgaa ccatatggaa tacaacgtga tctcgcggcc
      301 aatcctcttc ccctgaacga accgccgata caggaaatcc gggaggattg ttaataccga
      361 gcagagaagc aattcaatca tgacctctgc ctctccttgg atgccgcatg ctgccgtttg
      421 ccggcgctcc cgccctgcgc cggatctccg tcgccgccgg cggaacaggt ctcgatgttg
      481 aggcgatctt ccatcgtttc cataaggtca gactgccgag cgtccccggt cgcaggcggc
      541 gttttccgga cgttttcggc tggcgtggcg gccgatggca ggtcggttct aggtcggcgc
      601 cagccggcca tcttggccag tgaagcggcc attccggcca gcggcgtata aagatcggga
      661 atgggaacaa gtgcgagcag caaagccgcg acccacaacg cgttgttgtg cgtgaacagc
      721 gccagcagcg ccaggaccgc cacaacttcg aactgcagtt tgtgcgtgtt accgtgcgca
      781 agacgttccg gcagtgagtg aagcctgaaa tacaggctcc ccagcccgag caccgccagg
      841 acaaggaaaa tcgcgcacgc gatcaggaac gtgtccgact cgtccggtgc ggtgataaac
      901 atgggaagat ggtgaggggc cgctgggtgt atggcgttcg tcatctcagc ctggtctcga
      961 aaagcccgca acatcggcga tacgacccaa ggcgcaatac gccgcaccca cgcgccttcg
     1021 ggttcgcagc aaattcgtgc atcgggtcag atcatggagc cgcgtctcga aaggacgcaa
     1081 gtaagttact gtaatcggaa ttgccctgcc ggcgccccgg aaactacagg ccggcagctc
     1141 atatgctgcg atctcgcgaa cggcgcggag gagaaatttt gcaaacagtc cggatggccg
     1201 tcgcctgatt caacttgacc gaatgtcatc cggccttgtt gcacggttca tcgcgtccag
     1261 cagcgttttt gccgaaaacg gcttcacgag aaaagcgccg cagccggctg cggcagcggc
     1321 cttgcgcgtg gtttcagtgt cattcgcggt cacgagcacg accgggaccc ctattcctgc
     1381 gccggacaac caatacagca gctcgattcc cgaaatgcca cccaggtgga tgtccaaaat
     1441 cagacattcg gcttcggcga catctgcatc cgcctgcaac gcttccgccg aggtaaaggt
     1501 cctggtgcca agaccgtgcg cactgagcag gagttcgatt cctctaaggt attcgacgtc
     1561 gtcgtcaacg acgtagatga gcttaccgct ttgcatgctg gttcggcgtc tccgacctcc
     1621 cgccgcacaa tgcaatgatc gtttgtgtgc cgtctcggat aaaatacgaa aacgatgtcg
     1681 attatccagt tgccctcgcg gacaagctag gtatcacgcc ggtaacagtc ctcgcctgtg
     1741 ccgagtgtct cccccttgag ccgggcttcc cgctgaattc gcgaacgcac aaccgtaccc
     1801 gcgtcctgcg gtacaccgac agtgggcgct ccggacacga tcccgagccg caccgccaga
     1861 gacacaaggt cgatgaccga ccgcgcgtgg agcttttcac agatgtgctg tcggtgcgcc
     1921 ttgaccgtgc gctccgttat gccaagtgca tgtgccgtct gcttgttaag ctcgcctcga
     1981 accacgtagt cgaacacctc gcgttcccgc ggcgtcagac cctgcagcga taattgcgcg
     2041 ctcccaagcc attcacgctg tgctttttcg gcctc
//
LOCUS       DASUOM010000483         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_883728,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000483 DASUOM010000000
VERSION     DASUOM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883728"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFY27_10060"
     CDS             complement(<1..300)
                     /locus_tag="VFY27_10060"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HEX5765902.1"
                     /translation="MTSAIVSVTLFASPARGQLNEVIDAQVEADQAAAQSQQRIDETR
                     DRTQDAAANYAQSLAEAESLEKYNKQLAAQVLSQEAEIASIEKQLLDIETTNREIQ"
     gene            766..1479
                     /locus_tag="VFY27_10065"
     CDS             766..1479
                     /locus_tag="VFY27_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5765903.1"
                     /translation="MMDPYMGMVLIIEDDAAFRRFLRNTLAVQRYGVREAENGREGVA
                     AALDTKLGLIMLDLGLPDMDGMDVIRLIRSVSEVPILVVSNRGDEVAKVTALDLGADD
                     YISKPCTAEELTTRIRSLCRNRPTDDEQKTTKVFSNAGLTVDVALGVVTVEGRKIELS
                     DQEFGILREFVLHAGRALTHRRLLQAAWGREEAGDVLLLRACIRRIREKIELSPAQPR
                     HIVTEAGIGYRLEILNEST"
     gene            1481..1714
                     /locus_tag="VFY27_10070"
     CDS             1481..1714
                     /locus_tag="VFY27_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765904.1"
                     /translation="MASFAVSTPWRPITYKAVSPCQRPLDECVVYDAGNGRADVPDPR
                     GRRLLVDTSLPVMNNVAGERKLDGAPAIVRRSF"
     gene            1711..2304
                     /locus_tag="VFY27_10075"
     CDS             1711..2304
                     /locus_tag="VFY27_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX5765905.1"
                     /translation="MSLLVHRRSAASGVTDTLGAVGVCLALLALASGVALAAERFLHV
                     QNILLVFLPVILFAAVRYGFWMALSCSGLSIASSSYFLADPRFSFAVRDAENIWALLF
                     FLLVSGFTSTLAAQLRHRTEALQRNSRVTEELFAFSSMLAAISETEDLLRASARRLAH
                     TLGADVRIFIPEDGNLTTRGSPSGKANAARDELDAAR"
     gene            2291..3259
                     /locus_tag="VFY27_10080"
     CDS             2291..3259
                     /locus_tag="VFY27_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5765906.1"
                     /translation="MRHADAFEHGLPSGHGTGAPSNVAGLYLPLRTGRGTVGVIGLFP
                     HGGMEIRTSPETTLIDSLTNQVAINLERVQLGEKMQETRILAEADKLRTALLTSISHD
                     LKTPLASILGNVSSLAQYGKLYDEATRIEMLEMAEAETVRLSRFVDNLLHMTRIDAGG
                     LRPTLEMLDVSDLVGSALTRSEKLLHRHRLCTDLPLDLPMIDADFVLAEHVLVNLLDN
                     AAKYSPPGSEILVAAREADDRVDIYVQDEGPGIVAEDLDRIFQRFFRVVAADHRPAGT
                     GLGLAICKGFVEAMGGTICAINRTGKPGARFTITLPRSAYAEGSNR"
BASE COUNT          604 a          977 c         1016 g          718 t
ORIGIN      
        1 ctgaatctcg cggttggtcg tttcgatgtc gagaagctgc ttttcgattg acgctatctc
       61 ggcctcctgc gagagcacct gggccgccag ctgtttgttg tatttttcaa gactttccgc
      121 ctcagccaga gattgggcat agtttgcggc cgcatcctgt gtccgatccc tggtttcgtc
      181 gatgcgttgc tgggattgcg cggcagcttg gtctgcctcc acctgtgcat cgatgacttc
      241 gttgagctgt ccgcgagcag ggctggcaaa cagtgtgacg cttacgatcg ccgacgtcat
      301 agcggcagag agccccggga ttcgattcgg gcagctgctt gcagaacgtt ggacttgctg
      361 tccgcacacg gcgctcccgc gccagtgctg gcttgatgag cgtcgcattg tctgaactcc
      421 gtacataccg tttgtttaag gaacgtgcat aaatctgcac gccgcgggcg taggatttca
      481 attagccgga tagcgcctgt tcatatgaaa tgcataaagc cgaacctggc ctgatggagc
      541 gtgaggttca tatatggctt tcctatgcgc aattgtctgc atgggaattg aattcctatg
      601 cgttcctcct tcaatctgcc gaatcagcag cgtgtaattc atttccttta ttgcacacgg
      661 gttattgcac actgggtgta agtcctcgag ggctggcgga cactgaccgt ttgcccgccg
      721 agtccggcag gctgtcaacg tcgactttga gaggcgacgc gcacgatgat ggacccttac
      781 atgggcatgg tcctcattat cgaagacgac gcggcgtttc gccgttttct gcgcaacacg
      841 ctggcggttc agcgttacgg agttcgggaa gccgagaacg gtcgggaggg agtcgccgcg
      901 gcgttggaca ctaagctcgg gctgatcatg ctcgatctcg gtctgccaga catggacggg
      961 atggacgtga ttcgtctcat ccgctcggtg tccgaagtgc cgattctggt tgtctccaat
     1021 cgcggcgacg aagtagcgaa ggtcacggct ctggacttgg gggcggacga ttatatcagc
     1081 aaaccctgca ccgcggagga gcttacgacg cgtattcgca gcctttgccg gaatcgacct
     1141 accgacgatg agcagaagac aacgaaagtt ttctcgaacg cagggcttac ggtggatgtg
     1201 gcgctcggcg tcgtgacggt ggagggccgt aaaatcgaac tttcggacca ggagttcggc
     1261 atcttgcgcg aattcgtgct gcacgccgga agagccctga cacaccgccg tttgttacag
     1321 gcagcatggg gcagggaaga ggctggcgat gtgcttcttc ttcgtgcttg catacggcgg
     1381 attcgcgaga agatcgagct gtcgcccgct caaccgcggc acatcgtaac ggaagcggga
     1441 atcgggtaca gactggagat cctgaacgag tcgacatgac atggcgtcct tcgcagtgtc
     1501 gactccgtgg cggcccatta cctacaaggc ggtctcgccg tgtcagcgtc cgctcgacga
     1561 atgcgtggtg tatgatgccg gcaacgggcg cgctgacgtc cccgatcccc ggggacgacg
     1621 gttgctcgtc gacaccagcc ttccggtcat gaacaacgtc gcgggggagc gaaagctcga
     1681 cggcgcgccc gcgatcgtcc gtcgttcatt ttgagtctgc tcgtgcaccg tcgttccgcg
     1741 gcgagcggcg tgacggacac cctcggtgcc gtcggcgtct gcctggcgct tcttgcactc
     1801 gcctcgggcg tcgcgcttgc cgccgagcgc tttctgcacg tccagaacat tctgctcgta
     1861 tttctgccgg tcatcctctt cgccgccgtg cgctacggct tctggatggc actgagctgt
     1921 tcagggctga gcatcgccag cagcagctat tttctcgccg atccccgttt ttccttcgcc
     1981 gtaagggacg ccgagaacat ctgggcgttg ctgttctttc tgctcgtctc cggtttcacg
     2041 agcacgcttg cggcgcagct gcgccatcgc accgaggcgc tgcaacgaaa cagccgtgtg
     2101 acggaggaac tctttgcctt cagctccatg ctggcggcga tatccgagac tgaggatctg
     2161 ctgcgcgcgt cagcccggcg cctcgcgcac acgctcggag cggatgtccg gattttcatt
     2221 ccggaagacg ggaatctcac aactcgcggt agcccatccg gcaaagcaaa tgccgcacgc
     2281 gatgaactcg atgcggcacg ctgatgcttt tgagcacggc ctcccgtccg gtcacggaac
     2341 cggcgctcca agcaacgttg cgggcctgta tctgcctctc cgcacgggcc gcggcaccgt
     2401 cggcgtcatc ggccttttcc ctcacggtgg gatggagata cgcacgtcgc ctgagaccac
     2461 actgatcgat tccctgacca accaggtggc gataaacctg gagcgagtgc agctcggcga
     2521 gaagatgcag gaaacgcgca tactcgccga ggcggacaag ctgcgaacgg ctttgctgac
     2581 ttccatatcg cacgacctca agactccgct ggcgtccatt ctcggcaacg tcagtagcct
     2641 cgctcagtac ggcaagctgt acgatgaggc aactcgcatc gaaatgcttg aaatggcgga
     2701 agctgaaacg gtacggctga gccgctttgt cgataacctg ctgcacatga cgcgcatcga
     2761 cgcgggagga ttacgcccga cgctcgagat gctcgatgtc agcgacctcg tcggctcggc
     2821 gcttacgcga tcggaaaagc ttttgcaccg ccatcgatta tgcaccgacc ttcctctgga
     2881 tctgccgatg atcgacgccg atttcgttct cgcggagcac gtactggtca atctcttgga
     2941 taacgcggca aagtattcgc cgcccgggtc tgaaatcctc gtcgcagcgc gcgaggctga
     3001 cgatcgcgtg gacatctacg tgcaggatga agggcccgga atcgtcgcgg aggatctgga
     3061 ccggattttc cagcgtttct tccgcgtcgt ggcggccgat catagaccgg cagggaccgg
     3121 tctgggattg gcgatctgca agggattcgt cgaggccatg ggcggaacga tctgcgccat
     3181 caaccgcacc ggcaagccgg gtgcgcgctt cacgatcacc ctgccaaggt ctgcctatgc
     3241 ggaggggtcg aaccgatgag cgcgggcggc attcgggtcc tgctcgtcga ggacgatccg
     3301 gcgattcttc acttc
//
LOCUS       DASUOM010000484         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_611918,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000484 DASUOM010000000
VERSION     DASUOM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611918"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1336)
                     /locus_tag="VFY27_10085"
     CDS             complement(74..1336)
                     /locus_tag="VFY27_10085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5765907.1"
                     /translation="MTLKETGHSSGSSDTTGPLKGLRVLDLTRVLAGPWATQLLGDYG
                     ADVIKVERPGSGDDTRHWGPPWLSGRDGSRTGESAYFLSANRNKRSITIDIAHPSGQR
                     IVRELAAMSDVLIENFRAGSLARYKLDQDSLRDTFPRLIYCSITAYGQCGTRSSEPGY
                     DAMMQASAGLMSITGAPDDEGGSPQKVGVAISDIMAGMYATTAILAALHAREKTGQGQ
                     YIDVPLYDSQVAWLANQGMNYLVSGEVPRRLGTGHPNLVPYQAFKTSDGFLMLAVGND
                     RQFRSCAQCLGCPELADTEKFSTNASRVEHRGELIAILADAFRRRSTAEWLTELRRQR
                     VPAGPINDLATVFNGAEVAEAQLVRRLEHPLSESVPTIANPVRFATTPVDYRTAPPLL
                     GEHTRELLREELGYTESVIDELRGSGVI"
     gene            complement(1461..1934)
                     /locus_tag="VFY27_10090"
     CDS             complement(1461..1934)
                     /locus_tag="VFY27_10090"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX5765908.1"
                     /translation="MKNLSRQVLATLTSCIIAACATTGEPDAGSSGDVDFRGSDCILI
                     STIRDYTPLDDSNLLIYGPGKRAYFVTLVHSTFELRSSFRLQFSSRDGQLCPFGGDEI
                     VVGSFGHEAVGIRAISRVTEEQAEQLLIRYGRKEPAEQQAPAPEDVKGAEVEELG"
     gene            complement(2023..>2459)
                     /locus_tag="VFY27_10095"
     CDS             complement(2023..>2459)
                     /locus_tag="VFY27_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5765909.1"
                     /translation="TTADVEKALAAGSPLPLVDAREPARFEGRTEPIDAVAGHIPGAR
                     NLPFSSNLTAEGNWRSPAELREAWARVLGPPPEVPGDDNDSLAVMCGSGVTACHLALS
                     AGLAGLPLPRLYAGSWSEWIRERRPVATGPAGKGKEPGAEGS"
BASE COUNT          449 a          776 c          759 g          475 t
ORIGIN      
        1 tacctcgggc agccatggat aatggccggt catggccgca tgtggcggaa ccccggatgc
       61 tccgattcgc gactcatatg acgccggacc cccgcaactc gtcgataacc gattcggtgt
      121 agccgagttc ttcgcgaagc agctcgcgcg tatgttcgcc cagcaacggg ggcgccgtgc
      181 ggtagtcgac cggcgttgtc gcgaaacgca ccgggttcgc gatggtcggt accgattccg
      241 acagcggatg ctccaggcgc cgaaccagct gtgcctccgc tacctccgcg ccattgaaca
      301 ccgtcgcgag atcgttgatg ggtccggcgg gaacgcgttg ccggcgcagt tccgtcagcc
      361 actccgcggt actacggcgg cgaaatgcat cggcaagaat cgcgatgagt tcgccgcgat
      421 gctcgacccg ggacgcattg gtggaaaatt tttcggtgtc cgcgagttcc gggcatccaa
      481 ggcactgggc acaggagcgg aactggcggt cattgcccac cgccagcatg aggaaaccat
      541 cgctggtctt gaaagcctgg tacggcacga ggttcgggtg cccggtgccg agtcgccgcg
      601 gtacctcgcc cgacaccagg tagttcatgc cctggttggc gagccacgcc acctggctgt
      661 cgtaaagcgg cacatcgatg tactgcccct gtcctgtttt ctcccgcgcg tgcagggcgg
      721 caagtatggc ggtcgtcgcg tacatcccgg ccatgatatc gctgatggcg acaccgactt
      781 tctgcggcga gcctccctcg tcatcgggcg cgccggtaat gctcatcaat ccggcggaag
      841 cctgcatcat cgcatcgtaa cccggttcgg acgaacgcgt gccgcattgg ccgtaagcgg
      901 taatggaaca atagatgagc ctggggaacg tgtcgcgcag gctgtcctgg tccagcttat
      961 acctcgcgag agaaccggcg cgaaaattct cgatcagcac atcgctcatt gccgccagtt
     1021 cacggacgat ccgctgtccg gaaggatgcg cgatgtcgat ggtgatggag cgcttgttgc
     1081 gattcgcgga gaggaagtag gcggattccc ccgtccggct gccgtcgcgc ccggagagcc
     1141 agggaggccc ccaatggcga gtgtcgtcac cgctgccggg acgctctacc ttgatgacgt
     1201 cagcgccgta gtcgccgagc agttgtgtcg cccacggtcc tgcaagaacg cgggtgaggt
     1261 cgagcactcg tagtcccttg agcggtccgg tagtgtccga cgaacccgag gagtgtccgg
     1321 tttctttcag cgtcatctaa tgcctaaaaa taaaaacccg gccaaccctt acgggatggc
     1381 cgggtgggtc cgcacggaga ttgcggattg cgcggctaaa tcgactgaga caggatcttg
     1441 cgaccttgta ccaaaccgac tcagccaagc tcctcgacct cggcaccttt tacgtcttcc
     1501 ggtgccggag cctgttgttc agcaggttcc ttacgaccat accgaatcag taactgctct
     1561 gcctgctctt cggtcacgcg actgatggcg cggatcccga ctgcttcgtg accgaacgat
     1621 ccgaccacga tctcgtcccc gccgaaagga cagagctgcc cgtcccgtga cgaaaattgc
     1681 aagcgaaaag aggatcttag ctcaaatgtc gagtgaacca gggtgacaaa ataggcgcgt
     1741 ttcccgggtc cgtagatcaa cagattgcta tcgtccagcg gcgtatagtc tcggatagtc
     1801 gagatcagga tgcaatcgct gccgcggaaa tcgacgtcgc cgctgctgcc ggcatccggc
     1861 tcgccagtag tcgcacatgc tgcgataata catgaagtga gtgtcgcaag aacctgtcgg
     1921 gaaaggtttt tcattggatc cgtttttcac ctattccgtt agattgatga cgctgaaccc
     1981 gggcggaatg gcccgtttgg actgaaaggt cttgtattaa gttcagctac cctccgcgcc
     2041 cggctccttc cctttccccg ccggcccggt cgccacgggg cggcgctcgc ggatccactc
     2101 gctccaggac ccggcgtaca gtcgtggcaa cggcaggccg gcgaggccgg ctgagagcgc
     2161 caggtggcag gcggtaacgc cagacccgca catcaccgcc aaactgtcgt tgtcgtcccc
     2221 cggaacctcc ggtggaggcc cgaggaccct tgcccaggcc tcgcgcagtt cggccgggga
     2281 gcgccaattc ccctccgccg tcaggttgct gctgaagggg aggtttcggg cgcccggaat
     2341 gtgtcccgcc accgcgtcga tcggctctgt cctgccctcg aagcgcgccg gctcgcgcgc
     2401 atcgaccagc ggcagcgggc tgcccgccgc gagtgccttt tccacatctg ccgttgtta
//
LOCUS       DASUOM010000485         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1746486,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000485 DASUOM010000000
VERSION     DASUOM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746486"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFY27_10100"
     CDS             <1..465
                     /locus_tag="VFY27_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5765910.1"
                     /translation="RYAGTAAACGTILERIVAAPVADRPLKALEEVVACRQARQQVPG
                     FGHPVHHETDPRVERLLGIAREAGVDGHFIGALDLLAESVRKELAKPLVTNISAAIAA
                     VLGEAEIPARMMRGIVLTARCAGLVGHLLEEMDNPSADEMWLAARAAVESPD"
     gene            477..1289
                     /locus_tag="VFY27_10105"
     CDS             477..1289
                     /locus_tag="VFY27_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5765911.1"
                     /translation="MWVDVDGGRLRLHTSGNGPPILLLHGWPLDHRVFAPQVRFLERS
                     FRVTAFDRRGFGRSEAPPDLDRELDDMDRILDALRIETVHLLGMSQGARVALRFAVTR
                     PGSIRSLILQAPAVDGLVLDQPESERIPLDEFAALARAGQLDEVRRRWLAHPMMTLGP
                     DEDQLRRDILRIIGDYQGKDLLDYSPASQTFAHDVLAELPRLDRPCLILTGGHDTASR
                     REHARRLRESLPRAREVVFRRSGHLSNLQEADAYNREVADFCASVDAGAVLE"
     gene            complement(1313..2134)
                     /locus_tag="VFY27_10110"
     CDS             complement(1313..2134)
                     /locus_tag="VFY27_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765912.1"
                     /translation="MVAADDKSRKNGEYLTTLARGLSVLRSFTKERPEMTLSEVAVVT
                     GLSPAVARRCLNTLLELGYVGRKGKLFLLTPEVMGFASAFLESMNLEEVVRPYLQEVR
                     DSTGDSSSLAVLSHLDILYLVHVSTNRMVRLVAGFGTRFPAYPTSLGRVLLAHQPEPQ
                     IERYLAEVKMNKLTDKTVTSVTKLRRILNRCRRDGYASIQDELDYGIVSVAVPVFGAD
                     GHILAAINCSTATTRVNQEHMVETRLPQLRAAAKSIEIELRRYPILVHSIKSASS"
     gene            complement(2333..2779)
                     /locus_tag="VFY27_10115"
     CDS             complement(2333..2779)
                     /locus_tag="VFY27_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEX5765913.1"
                     /translation="MAAFPLETLFSAAGTLALAGWLVLGLAPLRYRAPRYVAIAVAVV
                     IAMLYAGLIGAFWAQGEGGFGSLAEVARLFGHPGLLLAGWVHYLAFDLLVGVWERDEA
                     RRIGLPQWALVPCLVLTFLLGPLGWLVFLAVRFFRLKTASPAAAPA"
     gene            2858..3769
                     /locus_tag="VFY27_10120"
     CDS             2858..3769
                     /locus_tag="VFY27_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX5765914.1"
                     /translation="MECTTRNTAEIGAPDHTIQQTTRSSPGIPVCVLMTAHTAPDGHG
                     SIDDLDRAIVNLAAIINAASHELLVLIRRFDERGGWLRWGFENCAEWLHWRCDLSLSA
                     AREKVRVAHALKALPAVAMSFARGELSYSKVRALTRVATCHNENHLLDFALHTTAARV
                     EERCRELRCGTADSTDEAVRAFARRSLTMRRDPSRGTMTIIVDLPLETGELIDKALDR
                     ARDAAASSDPVSAESSWAAQQADALVAIAQIYLGGNCDASAGAANNYMVTVHVDHAAL
                     THGKGRFRLSPPRRCTPIPPRRGGLAR"
     gene            complement(4050..>4636)
                     /gene="rlmJ"
                     /locus_tag="VFY27_10125"
     CDS             complement(4050..>4636)
                     /gene="rlmJ"
                     /locus_tag="VFY27_10125"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX5765915.1"
                     /translation="RVRAANPTGELTGYPGSPALAATLLREHDRLVCLELHPADHRLL
                     KAHFAADRRVQVHRRDAHEGLSALLPPAEKRGLVLLDPPYERSEEYTEVPASLRRAHA
                     RWPNGSYLVWYPLLAGNPHERLVANLAAGDIHRVLVQELRLHPEARGLRGSGILWINP
                     PWQVDAALKEAGTWLVEHLGALGASTRLEWWVSE"
BASE COUNT          890 a         1463 c         1479 g          804 t
ORIGIN      
        1 cgttacgccg gcaccgcggc ggcttgcggg acgatactgg agcgtatcgt tgcggcgccc
       61 gttgcggacc gtccgctaaa agcgcttgaa gaagtggtcg cctgcaggca ggcccgccaa
      121 caggtgccgg gtttcgggca ccccgttcac cacgagacgg atccgcgggt tgagcggctc
      181 ctcggcatcg cgagggaagc cggggttgac ggccatttca tcggtgcgct cgatctgctg
      241 gccgaaagcg ttcgcaagga actcgcaaag ccgctggtga cgaacatcag cgcagccatc
      301 gcagcggtgc ttggcgaggc cgaaattcct gctcgcatga tgcgaggcat cgtcctgacc
      361 gcgcgttgcg cagggctggt tggtcacctg ctcgaggaaa tggacaaccc gagcgcggat
      421 gagatgtggc tcgcggccag ggcggccgtc gagtctcctg actgacgcat ccggtaatgt
      481 gggtcgatgt cgacggcggc cggctgcggc tgcatacgtc gggcaacggt cctcccatcc
      541 tgctgctgca tggctggcca ctggaccacc gggtgtttgc cccccaggtc cggtttctcg
      601 agcgatcctt tcgcgtcact gcattcgacc gccgcggctt tggccggtcg gaggcgcctc
      661 cggatctcga ccgggaactg gatgacatgg acaggatcct agacgcttta cggatagaga
      721 cagtgcacct gctcggcatg tcgcaaggcg cccgcgttgc cttgcgcttc gccgtcacgc
      781 gaccgggcag catccgctcg ctgatcctgc aggcaccggc ggtcgatggc ctcgttctcg
      841 accagcccga gagcgaacgt atacccctcg atgagtttgc agcgctagcc cgtgccggcc
      901 agctggacga ggttcgcagg cgctggctcg cacacccgat gatgacgctt ggtcccgatg
      961 aggatcagtt gaggcgcgat atcctgcgga tcatcggcga ttaccaggga aaggaccttc
     1021 tcgactactc gccggcaagc cagacttttg cgcatgacgt gctcgccgaa ctaccgaggc
     1081 tggacaggcc ctgtctgatc ctgaccggcg ggcacgatac cgcctcgcgc cgcgaacatg
     1141 cgcgaagact gcgggaatcg ctgccccgag cccgcgaagt ggtgttcagg cgaagcggtc
     1201 acctgagcaa cctgcaggaa gcggacgctt acaaccgcga ggtcgcagac ttctgcgcga
     1261 gcgtcgatgc tggcgcagtc ctggagtagt cggagcggca ccggccggtc ggtcaggaac
     1321 tggcgctctt tatggagtga acgagaatcg ggtaacgccg gagctcgatt tctatgcttt
     1381 ttgcagcggc ccgcaattgc ggcagccggg tttccaccat gtgttcctgg ttgacgcggg
     1441 tcgttgcggt cgagcagttg atggccgcca ggatatgacc gtccgcgccg aacacgggaa
     1501 cggcgacgga tacgatgccg tagtcgagtt cgtcctgtat cgatgcgtag ccgtcgcgcc
     1561 tgcagcggtt caggatccgg cgaagcttcg ttacggaagt gacggtcttg tcggtgagct
     1621 tgttcatttt gacttcggca agataccgtt cgatctgcgg ctcgggctgg tgcgcgagca
     1681 gcactcgtcc cagtgacgtc ggatatgcag gaaagcgggt gccgaacccg gcgacgagcc
     1741 gcaccatgcg attggtcgac acgtgaacca ggtacaggat atcgagatgt gaaagcacgg
     1801 cgagcgagga actgtcgccc gtgctgtccc gaacctcctg caggtaaggg cgtaccactt
     1861 cctccaggtt catcgactcc aggaacgccg aagcgaatcc cattacttcg ggcgtcagca
     1921 ggaacagctt gcccttcctg ccgacgtagc ccagctccag caaggtattg aggcagcgcc
     1981 tggcaacggc cggactgaga ccggtcacga ccgccacctc gctcaaggtc atctccggcc
     2041 gttccttcgt gaaagaccgg aggaccgaca gtccgcgggc aagcgtcgtc aggtactcgc
     2101 cgttcttcct agatttatcg tcagcggcaa ccaaatgagg acgtctcttt gcatccggcg
     2161 cgtcgttatc gctgatgacg ggagacaaat caaccgaagt ttggcaggaa gggctctccg
     2221 tgaagggcct gcacgaaggt aaacgcgatc agtccgacct aagccagcga cccaagcgcg
     2281 accccggctc tcgcgtaagg caagcgggag tactgccgcg ctcagcctgt tactaggccg
     2341 gggcagctgc gggcgaagcc gtcttcaggc ggaagaagcg caccgccaga aagaccagcc
     2401 agccgagcgg cccgagcagg aacgtcaaca cgagacacgg caccagtgcc cattgcggaa
     2461 ggccgatacg gcgcgcctcg tcccgctccc acacgccgac cagcaagtcg aacgcgaggt
     2521 aatgcaccca gccggcaagc agcagcccgg ggtgcccgaa caggcgcgcg acctccgcaa
     2581 ggctgccgaa gccgccctcc ccctgcgccc agaacgcgcc gatcagccct gcgtaaagca
     2641 tggcgatgac gacggccacc gcgatcgcca cgtagcgcgg cgcgcgatag cggagcggag
     2701 ccagaccgag aaccagccag cctgccaagg caagcgtgcc ggcggcagaa aacaaagttt
     2761 ccagtggaaa tgcagccacg cgtatctcct cctcttgtag acactgtcta gttcacaagg
     2821 agcgacaata gcgggtgcaa gggcatctgg gcatctcgtg gaatgcacca cccgcaacac
     2881 cgccgaaatc ggcgcacctg atcacacgat ccagcagaca acgcgaagtt cgcccggaat
     2941 tcccgtctgc gtgcttatga ctgcccacac cgcacccgac ggccatggtt ccatagacga
     3001 cctcgaccgc gccatcgtca acctcgccgc tataatcaac gctgcgagcc acgaacttct
     3061 cgtcctgatc cgccggttcg acgagcgggg cggctggctc cgctggggct tcgagaactg
     3121 cgccgagtgg ctgcactggc gttgtgatct ctctttgagc gctgcccgtg agaaggtcag
     3181 ggtggcgcat gcgctgaaag cactgcctgc ggtcgcaatg tcatttgcca ggggcgagct
     3241 gtcgtattcg aaggtacgcg cgctgacgcg ggtagcaact tgccacaacg aaaatcactt
     3301 gctcgacttc gcattgcaca ccactgctgc gcgggtcgag gagcgctgcc gggaactccg
     3361 ctgcggtacc gcggattcca ctgacgaggc cgttcgtgct tttgcgcgcc gatcgcttac
     3421 gatgcggcgc gatccttcgc gtggaactat gacaatcata gtggacctgc ccctggagac
     3481 gggggagctg atcgataaag cactcgatcg tgcgagagat gcggcagcct cgtcggatcc
     3541 cgtgtctgcg gagtcgagtt gggcggcgca gcaagccgat gcgctcgtgg ctatcgcaca
     3601 aatatatcta ggcgggaact gtgatgcatc ggcgggtgca gcaaataact acatggtcac
     3661 cgtgcacgtc gatcacgcag cgctcactca tggtaaaggt cgcttcaggc tgagccctcc
     3721 gcggaggtgt acgccgatcc ctccgcggag gggtggttta gcacgctgac gaactaccag
     3781 cagggcgtga atccgtccga tatgacggcc gccagcagtc cgtgaacttc gcgtcgagcg
     3841 gagcgacgct ttacgtttga ataatccctc cgcggaggtg tcagtagtac ctcctgcgtt
     3901 caggtattac cccactcgat caggaacagc cctccacata gtccacctgc ggtggcggga
     3961 tcagcaggcg acttgatacc cggcgcaggc tcctggcatg cggacagcgt gaggctgagc
     4021 acggcaagga acgggatcaa tggacaagct cattccgaaa cccaccattc gagtcgggtg
     4081 cttgcgccaa gcgcacccag gtgttctacc agccatgtgc ctgcctcctt gagtgcggcg
     4141 tccacctgcc agggcggatt gatccagagg attccggaac cacgaaggcc gcgcgcctcg
     4201 ggatggaggc gcagctcctg caccagtacc cggtggatgt ccccggcggc aagattcgcc
     4261 accaggcgct catgcggatt ccccgcaagc aatggatacc acaccaggta gcttccattc
     4321 ggccaccgcg catgagcgcg gcgcagcgac gcgggcacct cggtgtactc ctcgctgcgc
     4381 tcatagggtg gatcaagcaa cacgagaccg cgcttttccg cgggcggaag cagcgcggaa
     4441 aggccctcgt gcgcgtcccg ccgatgcacc tgcaccctcc tgtcggctgc gaagtgcgct
     4501 ttgagaagcc ggtggtcggc cggatgcagt tccaggcaga ccaggcggtc gtgttcgcgc
     4561 agcagggtcg ccgcgagcgc aggtgagccg ggataaccag taagctcccc tgtcggattc
     4621 gcggcgcgca cacgtt
//
LOCUS       DASUOM010000486         5037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_417882,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000486 DASUOM010000000
VERSION     DASUOM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417882"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFY27_10130"
     CDS             <1..793
                     /locus_tag="VFY27_10130"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HEX5765916.1"
                     /translation="GILGRYPLSADSSGQWRLPDKLNEISGLALTDDARLLAVTDESA
                     IVYELDYSEGRLVKAFALGEPTERDDFEGIAWFDGRVWLVTSNGKIYESGEGEDGERV
                     TFEAFETGIGKLCEIEGLAYRKSDSTLLVLCKNIRKKSDLESLAIFAWSTITRELVPG
                     KTIAMPDREIAAALRMARLNPSGIAIDGRSGNMLIVAARQHALIEVDADGGFVAARVL
                     PLTARHPQAEGIEILPSGGLLIADEGGGHRARLALYRPADRSNDE"
     gene            790..1791
                     /locus_tag="VFY27_10135"
     CDS             790..1791
                     /locus_tag="VFY27_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765917.1"
                     /translation="MNSDALRFSPADSGRSIIFVHGRDFKPAADELLDLTLAAVAAGL
                     ERDCPEMLVQFHSVEKRLAYYGDISNEWLRKSGRHYDEKLDIGDRRQALQELGALKKR
                     KHFGLNRYERIPGKTSLREFAADLAAPVLGSIGLGGAVISRIAADVNEYWDAATDYGL
                     RIRERVRDELSAALEQGRNVMLVSHGTGCIVCYDVLWQMSHHPDYAPQYEWMKIDQWL
                     TLGAPLGDTTVRKRIFGAQSKGRGKYPANVLAWHNVSAEDDYLCHDNTLSDDYGEMLR
                     LRLVSSIRDYHIYNLAVRYGKSNPHSSIGYLIHPRVTKIVADWLRRDNDARLTKGIF"
     gene            complement(1772..2830)
                     /locus_tag="VFY27_10140"
     CDS             complement(1772..2830)
                     /locus_tag="VFY27_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765918.1"
                     /translation="MTRPILLLALAFALVPAPALAQDAGEAARWPLFQNQDTLRVRIE
                     APLSTLMNKRSDTEYLDGTFAYTDAAGQEVELDLKLRTRGRYRRQATTCDLPPIRLNF
                     PKKQVEGTEFAGQDKLKLVTHCQENRDRYEQYVLKEYLAYRILNTVTDSSFRARLLSI
                     DYVDTDRNNEIDTRYGFVIEDDELLAERIGMTVLKVPNIRYGQVDEAQGALVAVFQYL
                     IGNTDFSMYAGAKDDDCCHNIVLFVKETKGYLPIPYDFDFSGLVNATYAEPNPKLPIK
                     KVTRRLYRGMCEHNAHVDAALATFREKEAAIRELVTSMEALDEGMRKEALKFIDRFYK
                     DTATPESVDRSLIRRCLS"
     gene            3062..4909
                     /locus_tag="VFY27_10145"
     CDS             3062..4909
                     /locus_tag="VFY27_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5765919.1"
                     /translation="MQIVIGIPRRLAAAVAALLLTASLAPWAAPAEDWRFTGVDRVVA
                     VSDVHGAYASMVRTLQNAGVLEGEAWDGGATHLVVTGDILDRGPDSRKVMDLLMRLEN
                     EALESGGRVHLLLGNHEVMNLTGDLRYVAAAEYEAFAGDENPADRERAFEIFRAARAA
                     EPDVEVLRREFEQRAPPGYFGHRKAFASDGEYGKWLLEKPLVVVINDTAFVHGGLSPM
                     VAELGLDGLNRQLKSEVSQYATRFEAVAAAGLIDVTETFHLRGPLLESLPDDTQRPAE
                     LKQAIADVISLEDASVHDSASPVWYRGNVGCSPLIENDKLDAALEAIGAKRVVIGHTP
                     TQSRQVLERLDGRVVEIDTGMLTAAYGGSGHALIIESNPGGDSLAVASEMEGRRAAPA
                     PHPRLSALEAKSMTVDELEALLRDGDIGSPAEDEAGRATVKVKSGSAELDALFIRNPR
                     KGFVPELAAYRLDRLLGLEMVPVTVAREVDGDAGVLQLLAANAKHEQSRRETGDGYQA
                     WCPLPEQWNAMYIFDALMHNAPRAPQNIRYSPGTWQLMLTGHDESFGTRSSRPAWLKE
                     APLQVGNAWKEALSALNDEVLESQLADVLDKRRLSALKKRRDALLEGTL"
BASE COUNT          941 a         1537 c         1666 g          893 t
ORIGIN      
        1 cggcatcctc gggcgctacc cgctatccgc agacagctcc gggcagtgga gactgccgga
       61 caagctgaac gaaatttccg gcctcgccct gaccgacgat gcgcggctgc tcgccgtcac
      121 ggacgagagc gcgatcgtct acgaactcga ttactcggaa ggccggctcg taaaggcctt
      181 tgccctgggc gagccgaccg aacgcgatga tttcgagggc atcgcctggt tcgacgggcg
      241 ggtatggctg gtcacgagca atggcaagat ctacgaatcc ggcgaagggg aggacggcga
      301 gcgcgtgacc ttcgaagcgt tcgagactgg cattggcaaa ttgtgtgaga tcgagggcct
      361 cgcctaccgg aagagcgaca gtacgctgct ggtgctgtgc aagaatatcc gcaagaagtc
      421 ggacctcgag agcctggcga tattcgcctg gtccaccatc acccgcgagc ttgtccccgg
      481 gaagacgatc gcaatgccgg atcgcgaaat tgcggccgcc ctgcgcatgg cgcggctcaa
      541 cccctccggc atcgccatcg acgggagaag cggaaacatg ctgatcgtcg cggcacggca
      601 gcacgccctt attgaagtgg acgcggacgg cggtttcgtc gcggcacggg tgctgccgct
      661 tacggcgcgc cacccgcagg cggagggcat cgagatccta ccctccggag gccttttgat
      721 tgcggacgag ggcggagggc accgcgcccg ccttgccctt taccggcccg cagacaggag
      781 taacgacgag tgaacagcga tgcgctgcga ttcagccccg ccgattccgg ccgcagcatc
      841 attttcgtcc acgggcgcga cttcaagccc gcggcggacg agcttctgga cctgaccctc
      901 gccgcggttg cagcgggact ggagcgcgat tgcccggaga tgctggtcca gtttcactcg
      961 gtcgaaaagc ggcttgccta ctacggtgac atcagcaacg agtggctgcg caaatccggc
     1021 aggcactacg acgagaagct ggacatcggc gaccggcggc aagctctgca ggaactgggc
     1081 gccctgaaga aacgcaagca cttcggcctc aaccgctacg agcgcattcc cggcaagacg
     1141 tcgctacgcg agttcgccgc cgacctcgcc gcgccggtgc tggggtcgat aggcctgggc
     1201 ggcgcggtca tcagcaggat cgcagcggac gtgaacgagt actgggacgc cgctaccgat
     1261 tacgggctgc gcatccgcga acgcgtaagg gacgagcttt ccgcggcgct ggagcaaggg
     1321 cggaacgtaa tgctggtttc gcacggcacc ggttgcatcg tctgctacga cgtgctgtgg
     1381 cagatgtcac atcacccgga ctacgcgccg cagtacgaat ggatgaagat cgaccagtgg
     1441 ttgacactcg gcgcgccgct gggggatacg actgtgcgca agcgcatttt cggggcgcag
     1501 tcgaagggca gggggaagta cccggccaac gttctcgcct ggcacaacgt gtcagcggag
     1561 gacgactacc tctgtcacga caacacgctg tccgacgact acggggagat gctgcggttg
     1621 cgtctcgtca gcagtatccg cgattaccac atttacaacc tcgcggtgcg ctatggaaaa
     1681 tcgaacccgc acagctccat cggctatctc attcatcctc gcgtgacgaa aatcgtcgcc
     1741 gattggctgc gccgcgacaa cgacgccagg ctcacgaaag gcatcttctg atcagggacc
     1801 ggtcgacgga ttccggggtt gcggtgtcct tatagaagcg gtcgataaat ttgagcgcct
     1861 ccttgcgcat cccctcgtcc agcgcctcca tcgaagtcac gagctccctg atggcggctt
     1921 ccttctcgcg aaaagtggcc agcgccgcat ccacatgggc gttgtgttcg cacatgccgc
     1981 ggtacagtct ccgggtcacc ttcttgatcg gcagcttcgg gttcggctct gcgtaggtgg
     2041 cattgacgag cccggaaaaa tcgaagtcgt acgggatcgg caggtagcct ttcgtctctt
     2101 tcacgaacag cacgatgtta tggcagcagt cgtcgtcctt ggccccggcg tacatagaga
     2161 aatcggtgtt gccgatgaga tattggaaaa cagcgaccag cgccccctgc gcttcatcga
     2221 cctggccgta ccggatgttc ggcaccttga gcaccgtcat gccgattcgc tccgccagca
     2281 gttcgtcatc ctcgatgacg aagccgtaac gggtgtcgat ctcgttattg cgatccgtat
     2341 cgacgtagtc gatgctgagc agcctcgcgc ggaagctcga gtccgtcacc gtgttaagga
     2401 tcctgtaggc aaggtattcc ttgagcacgt actgctcgta ccggtcgcgg ttctcctggc
     2461 aatgcgtcac cagtttcagt ttgtcctgcc cggcgaactc ggtaccctcc acctgcttct
     2521 tcgggaagtt gagcctgatg ggcggcaaat cgcaggtagt tgcttgcctt cggtacctgc
     2581 cgcgcgtccg cagtttgaga tcgagctcta cctcctgtcc ggcagcgtcg gtataggcga
     2641 acgtgccgtc gaggtattcg gtgtcgcttc gcttgttcat gagcgtggag agtggtgctt
     2701 cgatgcgcac ccgcagcgtg tcctggttct ggaacagcgg ccagcgcgcc gcttccccgg
     2761 catcctgcgc aagcgcaggc gcaggaacga gcgcgaatgc aagagccagc aacaagattg
     2821 gtctggtcac gattattgtc ctcagcaaaa gcgatcctgg ttcgcccatc cggagtctgt
     2881 ttctttcgtg gtgcggtcgc ggcgcacacc ccctatggca caaggccagg gtgtgccgcg
     2941 aatactacca aagcaggctt cgtgaatgag gatgtcattc ggggaaggcc cggctattgc
     3001 ccggggatcg cagcgggact ataaaccgca cacgccctag ccttcgggag cgcttgaaaa
     3061 gatgcagatc gtgatcggga ttccccgccg cctggcggcc gcagtcgcag cgttgctgtt
     3121 aacggcgtcg cttgcgccat gggccgcgcc ggcggaagac tggcgcttta cgggcgtcga
     3181 tcgcgtcgtt gccgtcagcg acgtccacgg cgcctatgcg tccatggtcc ggaccctgca
     3241 gaacgcagga gtgctggagg gcgaagcgtg ggacggcggc gcgactcacc tggtcgtcac
     3301 gggcgacatc ctcgatcgtg gcccggattc gcgcaaggtg atggacctgc tgatgcggct
     3361 ggagaacgag gcgcttgaat ccggcgggcg cgttcacctg ctgctcggta accacgaggt
     3421 aatgaacctg accggtgacc ttcgctacgt cgccgcggcg gaatacgagg cgttcgccgg
     3481 tgatgagaac ccggccgatc gcgagcgggc attcgagatc ttccgtgccg cgcgtgccgc
     3541 cgaaccggac gtggaggtcc tccggcgcga gttcgagcag cgtgcgccgc cggggtactt
     3601 cgggcatcgc aaggccttcg cgagcgatgg agaatacggc aagtggctgc tggagaagcc
     3661 gctggtcgtt gtgatcaacg acaccgcgtt cgtgcacggc gggttgtcgc caatggttgc
     3721 ggagctgggc ctcgatggcc tcaatcgtca actgaagtcg gaagtctcac agtatgccac
     3781 ccgcttcgaa gcggtggccg cggccgggct catcgacgtg acggaaacct ttcacctgcg
     3841 cgggccgttg ctcgagtcgt tgcccgacga cacgcaacgc ccggcggaac tgaagcaggc
     3901 gattgccgac gtcatcagcc tggaggacgc cagcgtgcac gacagtgcga gcccggtctg
     3961 gtatcgcggc aacgtcggct gcagcccctt gatcgaaaac gacaagctgg atgcggcact
     4021 ggaagcgatc ggtgcgaagc gcgtggtaat cggccacacg ccgacgcagt cgcggcaggt
     4081 gctggagcgg ctggatggac gggtcgtcga gatcgatacg gggatgttga cggccgcgta
     4141 cggcggtagc gggcacgcgc tgatcatcga gtcgaacccc ggcggcgaca gcctggcagt
     4201 ggcaagcgag atggaggggc gcagggccgc gcctgcgccg catccgaggt tatcggcgct
     4261 cgaagcgaaa tccatgacgg tggacgaact ggaggccttg ctgcgggatg gggatatcgg
     4321 gagtcccgcc gaggacgagg cgggccgggc gaccgtcaaa gtgaaaagcg gcagtgcgga
     4381 actggacgcg ctgttcatac gcaatccccg caaagggttt gttccggaac tcgcggctta
     4441 ccgtctcgac cggttgctcg ggctcgagat ggtgcctgtc accgttgccc gcgaggtgga
     4501 cggagacgcg ggagtactgc agctgcttgc cgcgaacgcg aaacacgagc agtcgcgcag
     4561 ggagacgggc gacggctacc aggcgtggtg cccgctgccc gaacaatgga acgcgatgta
     4621 catcttcgac gcgctgatgc acaacgcgcc gcgagctccg cagaatatcc gctacagccc
     4681 cggtacctgg cagctgatgc tgacggggca cgacgagtcc ttcggcacca ggagcagccg
     4741 cccggcgtgg ctcaaggaag cgcctttgca ggtcggcaac gcgtggaagg aagcgctatc
     4801 ggcgctgaac gacgaggtgc tcgagtcaca gctcgccgac gtcctcgaca aacgccgcct
     4861 gagtgccctg aaaaagcgcc gcgacgcgct cctggaagga accctgtagg agctcgccat
     4921 gcgagcgagt tccatgcggt ttcttcggca gccgcgaact cgcccgctgc ggcgggctcc
     4981 tacagcgctc gatttcggcc gtagcgaaag tcccggactc gtcttacggg cgacaat
//
LOCUS       DASUOM010000487         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_300454,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000487 DASUOM010000000
VERSION     DASUOM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300454"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFY27_10150"
     CDS             complement(<1..553)
                     /locus_tag="VFY27_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEX5765920.1"
                     /translation="MAWNDSGNGKDPWKKDDKEPHDLDRIVQNWQRRLSAIFGGRGGG
                     TASRTPGGSAGAWALGVLLLVAWALTGLYRVDEAERGIVQRFGAYTVTAMPGLHWHLP
                     YPIETVDIVNTEAVANYSFRTEILTADESYVFIQMVVQYRRSDPVKFSFEVVDPEMTV
                     QDVTESALREVVGTSSLQVLVTER"
     gene            complement(562..1881)
                     /gene="hflX"
                     /locus_tag="VFY27_10155"
     CDS             complement(562..1881)
                     /gene="hflX"
                     /locus_tag="VFY27_10155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HEX5765921.1"
                     /translation="MFERPRSGERAILVHATPDGAPDRLEREEFAELATSAGARVVDE
                     LVSSRKTPDSRFFIGKGKVEELQQRIAQHEAELVISSAPLSPSQERNLEKALECRVLD
                     RSGLILDIFATRARSFEGKLQVELAQLRHLSTRLVRGWSHLERQKGGIGLRGPGETQL
                     ETDRRLIGKRIRQLNSRLETVDSRREMNRQNRLRAEIPTVAIVGYTNAGKSTLFNALT
                     EAGVYVEDQLFATLDPTMRRLELPDRSRIILADTVGFVRDLPHELIAAFRSTLQETRE
                     ADLILHLIDASDENRWQRVRQVNAVLKQLDADRVPQIRVYNKIDKLDRPPRRANKRYG
                     KGRAVWLSAVTGEGIPLLLQAISERLQREKVRGIVHLHPNQGRQRALLFDMGAVSQEI
                     PTDDGGWTLELNMGERDFHRFVKRENLPADILERRGADQPPGAATQR"
     gene            complement(1914..2162)
                     /gene="hfq"
                     /locus_tag="VFY27_10160"
     CDS             complement(1914..2162)
                     /gene="hfq"
                     /locus_tag="VFY27_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020192568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5765922.1"
                     /translation="MAKGQSLQDPFLNILRKEKVPVSIYLVNGIKLQGQVDSFDQFVV
                     LLKNSVNQMVYKHAISTVVPARNVRLPLPEDEAGDSDD"
     gene            complement(2491..3411)
                     /gene="miaA"
                     /locus_tag="VFY27_10165"
     CDS             complement(2491..3411)
                     /gene="miaA"
                     /locus_tag="VFY27_10165"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5765923.1"
                     /translation="MGPTASGKTELALRLSDTLPVDIISVDSALVYRGMDIGTAKPDE
                     ETLRRYPHRLLDIREPEDSYSAGDFVRDATAAITDIQDAGRVPLLVGGTMMYFRALTR
                     GMARLPPADPRIREAIDAEAASDGWPALHRKLAAVDTTAAARISANDSQRIQRALEVY
                     QTSGRTITEWHRIGQPEGVRERFLKFGLIPRIRDVLHERIEQRLSTMLRNGFLDEVRR
                     LRGRPGLSARSPSMRAVGYRQLWSHLDGECELDTAVARALAATRQLAKRQLTWLRSES
                     GLYALDPLEPAAIATIVSTVEVQTGRETNQ"
     gene            complement(3446..>4428)
                     /gene="mutL"
                     /locus_tag="VFY27_10170"
     CDS             complement(3446..>4428)
                     /gene="mutL"
                     /locus_tag="VFY27_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717213.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX5765924.1"
                     /translation="TLSHAVRHAYRDVMFHGRYPAYVLHLSIDPAVVDANAHPAKLEV
                     RFRDGRAVHGAVSQAIEYALSGTRAGSRETTPVASVLVDQAFFRQTAMPLATRRGGNG
                     VAEALQGYEILTNTSTARAISAPANSARRDAPPLGFAIAQLAGIYILAQNDSGLVVVD
                     MHAAHERILYEQLKHAYDEKQFVRQPLLVPVTVAVSEREAELAEQAGGVFERLGLKLD
                     RVGPASVIVREIPALLKQSDVEALVRDILSDLQEAGASNRIEDRCHEFLATMACHHAV
                     RANRTLSIAEMNALLRAMESTEKSDQCNHGRPTWTTVSLADLDRLFLRGQ"
BASE COUNT          796 a         1388 c         1330 g          914 t
ORIGIN      
        1 gtcgctcggt aaccagcacc tgcaacgaac tcgtgccgac gacctcgcga agcgcgcttt
       61 cggtgacgtc ctgcacggtc atttccgggt caactacttc gaaactgaat ttgaccggat
      121 ccgacctgcg gtactgcacc accatctgga tgaacacgta ggattcgtcg gcggtcagga
      181 tctcggtgcg gaaagagtag ttggcgacgg cctcggtgtt tacgatgtcg acggtttcga
      241 tcgggtacgg aaggtgccag tgaagtcccg gcatcgcggt gacggtgtag gcaccgaagc
      301 gctgcacgat gccccgctcg gcttcatcga cccggtagag ccctgtgagc gcccacgcaa
      361 ccagcaacaa aacgccgagt gcccaggcgc cggccgagcc gccgggtgtc cgcgaagccg
      421 tgccgccgcc ccggccgccg aaaatcgcac tcagccggcg ctgccagttc tgtacgatcc
      481 ggtcgagatc gtgcggctcc ttatcgtctt tcttccaggg gtcttttccg ttaccagaat
      541 cgttccaagc catgctcttc tctatctttg tgttgcggcg ccgggcggct ggtccgctcc
      601 cctgcgctcc agaatgtcag caggcaggtt ctcacgtttc acaaaccggt ggaaatcccg
      661 ctcacccatg ttcagctcca gtgtccagcc gccatcgtcg gtcgggattt cctgggaaac
      721 ggcacccatg tcgaacagca gtgcgcgttg gcggccctgg ttggggtgaa ggtgaacgat
      781 gccccgcact ttctcccgct gcaggcgttc ggatatcgcc tgcagcaata gaggaatgcc
      841 ttctccggtc acggcggata gccacaccgc acggcccttg ccgtaacgct tgttcgcccg
      901 ccgtggcggc cggtccagct tgtcgatctt gttgtataca cgaatttgcg gcacccggtc
      961 ggcatccagc tgtttcagga ccgcgttcac ctggcgcact cgctgccagc gattctcgtc
     1021 gctcgcgtcg atcaggtgca ggatgaggtc cgcttcgcgc gtctcctgca gggtcgagcg
     1081 gaacgcggcg atcaactcgt gcggcaggtc gcgcacgaaa cccacggtat ccgcaaggat
     1141 tatccggctg cggtccggta gttccagccg ccgcatggta gggtccagcg tcgcgaacaa
     1201 ctggtcctcg acgtaaacgc ctgcctctgt gagcgcattg aacaatgtcg acttgccggc
     1261 gttcgtgtaa cccacgatcg caacggtcgg tatttccgcg cgaagccggt tctgccggtt
     1321 catttcccgc cggctgtcga ccgtttcgag gcgcgagttg agctggcgaa tgcgcttgcc
     1381 gatgagccgc cggtctgtct cgagctgggt ttcacccggc cccctgagac caatgccgcc
     1441 cttctggcgc tcgaggtggc tccagccgcg taccagccgc gtcgacaagt ggcgaagctg
     1501 cgccagttcg acttgcagct tgccttcgaa actgcgtgcg cgggtagcga aaatgtcgag
     1561 tatcagcccg ctgcgatcga gcacgcggca ctccagcgcc ttctcgaggt tgcgttcctg
     1621 gctcgggctc aacggcgcac tggaaatgac cagttccgct tcgtgctgcg cgatgcgctg
     1681 ttgcagttcc tcgactttgc ccttgcctat gaagaagcgt gagtcgggag tcttgcgtga
     1741 gcttacgagt tcgtcgacca cgcgagcacc tgccgatgtt gcaagctcgg caaattcctc
     1801 gcgttccaat ctatcggggg ccccatcggg tgtcgcatga accagaatgg cacgttcgcc
     1861 gcttcgcggt cgctcaaaca atcaggacct ccaggtccgg cgacaggttg atctcagtcg
     1921 tcactgtctc ctgcttcatc ctccggcagc ggtaagcgca cgttgcgcgc cggtacgaca
     1981 gtggatattg cgtgcttgta caccatctgg tttacgctgt tcttcagcaa cacaacgaac
     2041 tggtcgaaag aatcgacctg tccctgcagc ttgatcccgt tcaccaggta gatggaaaca
     2101 ggtaccttct ccttgcgtag aatattgagg aacgggtcct gtaacgattg cccttttgcc
     2161 atattgggtc tccttattct tgttgtagct caaatccagg caatcacatt catgggacac
     2221 ggcgatccga tggcgtcgga tgccaatatg ccatgcccgg aagcctccgc ggcagactta
     2281 ccggcagcga acaggcacgt cgacgaacct cgtccttcga tggatgcata actgtgattc
     2341 atcatcatcc aggtcacatt catacatgaa tccggagcgg gaaacggtac gttaatggcg
     2401 aattcgcggc aggtatctta tctatcttgc aggctccttg cccagagcga atagaaattc
     2461 tagcacagct tcagtatctg caacccggcc ttattggttc gtttcccggc cggtttgcac
     2521 ttccaccgtg gagacaatgg tggctatcgc tgccggttca agagggtcga gagcataaag
     2581 cccggattcg ctgcgcagcc aggtcaactg gcgcttggca agttgccggg tagcggccag
     2641 cgcccgcgcc actgccgtgt ccagctcgca ttcgccatcg aggtgtgacc aaagctggcg
     2701 ataacccaca gcgcgcatcg agggactgcg ggcggacaag ccggggcgcc cgcgaaggcg
     2761 ccggacttca tccaggaagc cgttccgcag catcgtcgaa aggcgctgct cgatgcgttc
     2821 gtggaggacg tcccggatac gcggaataag accgaacttc aaaaaacgct cgcgaacccc
     2881 ctccggctgc ccgatgcggt gccattccgt aattgtccgg ccgctggttt ggtatacctc
     2941 cagcgcgcgc tgaattcgct gactatcgtt cgcactgatc ctcgccgcgg cagtcgtgtc
     3001 gactgccgcg agtttgcgat gtaacgccgg ccagccatcg gacgcggctt cggcatcgat
     3061 ggcctcccgg atgcgcgggt cggccggagg cagtcgcgcc attccgcggg ttagcgcgcg
     3121 gaagtacatc atcgtgccac cgacgagcag cggtacacgc ccggcgtcct ggatgtccgt
     3181 tatcgcggcg gttgcatccc gcacgaagtc cccggcggag taactgtctt ctggctcccg
     3241 gatatccagg agtcgatgcg ggtagcgccg cagcgtttcc tcgtcgggtt tggccgtacc
     3301 gatgtccatc ccccggtaaa ccagcgcgga atcgacgctg atgatgtcga cgggtagtgt
     3361 gtcggacagc cggagcgcca gctcggtctt gcctgacgcg gttgggccca tgaggcaaac
     3421 ggcaacgcga tttgcgggca ctgttttatt gtccgcggag gaacagcctg tctagatccg
     3481 ccaggctgac cgtggtccag gtcggcctgc catggttgca ctggtccgat ttctccgtcg
     3541 attccattgc acgcaacagt gcgttcattt cggcaatgga aagtgtcctg ttagcacgaa
     3601 cggcatgatg gcacgccatc gtcgcaagaa actcgtggca tcggtcctca atgcgattgc
     3661 tggcgccagc ctcctgcagg tctgaaagaa tgtcccgcac gagcgcctcc acgtccgact
     3721 gtttcagcaa ggccggtatc tcgcgaacaa tgacgctcgc cggtccgacg cggtcaagtt
     3781 tcagccccag cctctcgaat accccgcccg cctgttcagc aagttcagct tcgcgctccg
     3841 ataccgcgac ggtgaccggg accagcaacg gctgccggac aaactgcttt tcgtcatacg
     3901 cgtgcttcag ttgctcataa aggatgcgtt cgtgcgctgc atgcatgtcg accacgacga
     3961 gcccgctgtc gttctgggcg aggatgtata tgcctgcgag ctgtgcgatc gcgaagccga
     4021 gcggtggcgc atctcgccgt gcggaatttg caggtgcgga aatggcacgc gcagtgctcg
     4081 tgttggtgag aatttcataa ccctgcagcg cctcggccac gccgttgcct cccctgcgcg
     4141 tggcgagagg catcgcggtc tgccggaaga acgcctgatc cacgagaact gacgccaccg
     4201 gcgtcgtctc gcggctcccg gcgcgcgtcc cggaaagagc gtactcgata gcctgcgata
     4261 cggcgccgtg caccgcgcgt ccatcgcgga aacgtacttc gagtttcgcg ggatgcgcat
     4321 tcgcgtcgac caccgcgggg tcaatggaca ggtgcaacac gtacgcggga tagcggccgt
     4381 gaaacatcac gtcacggtag gcgtgcctca cggcatggct gagcgtcc
//
LOCUS       DASUOM010000488         7398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_937674,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000488 DASUOM010000000
VERSION     DASUOM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937674"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFY27_10175"
     CDS             complement(<1..231)
                     /locus_tag="VFY27_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEX5765925.1"
                     /translation="MDTPPVEIRLRKNSRVLSLEFEDGSRFDLPFEYLRVNSPSAEVR
                     GHGSGEEVLQTGKENVVVTGVEPIGHYAIRLHF"
     gene            complement(264..1601)
                     /gene="hslU"
                     /locus_tag="VFY27_10180"
     CDS             complement(264..1601)
                     /gene="hslU"
                     /locus_tag="VFY27_10180"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5765926.1"
                     /translation="MSTMTPREIVQELDKHIIGQDEAKRAVAIALRNRWRRVQVDEPL
                     RSEITPKNILMIGPTGVGKTEISRRLARLARAPFIKVEATKFTEIGYVGREVDSIIRD
                     LMDVAVKMIREEAMGKVRHRAEDAAEERVLDALLPPPRNRVPEDDNRLLRGESDTRQK
                     FRKMLREGQLDDREIEVALQAMPMGVEIMAPPGMEEMTSQLQGMFQNLSGNRQKTRKL
                     KVKEAMKLLTDEEAAKMVDEEELKAQALEAVEQQGIVFLDELDKVARRSETMGADVSR
                     EGVQRDLLPLVEGSTINTKYGMVRTDHILFIASGAFHVSKPSDLIPELQGRFPIRVEL
                     KSLKTEDFVRILTEPDASLTAQYSALLGTEDVRLEFTDGGVRRLAEVAYHVNENTENI
                     GARRLHTVMERLLETVSFEASDKSGTTVRIDDDYVDDHLAELSRDEDLSRYIL"
     gene            complement(1605..2159)
                     /gene="hslV"
                     /locus_tag="VFY27_10185"
     CDS             complement(1605..2159)
                     /gene="hslV"
                     /locus_tag="VFY27_10185"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5765927.1"
                     /translation="MEQFRGTTILSVRRDGRVAIGGDGQVSMGNTVMKGNARKVRTLA
                     EGRVIAGFAGGTADAFTLFELFESKLERYGNLTRAAIELAKDWRTDRRLRRLEALLCV
                     ADAESSFVVSGNGDVIEPEHDVMAIGSGGLFAQAAALALVRNTELEARAIVEKALGIA
                     GDICVYTNHNIIVEELPPAEVKQD"
     gene            complement(2280..3188)
                     /gene="xerC"
                     /locus_tag="VFY27_10190"
     CDS             complement(2280..3188)
                     /gene="xerC"
                     /locus_tag="VFY27_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5765928.1"
                     /translation="MPDSEKEWIERFIRHLDYERRLSKLTCEHYGRDLEAFRLWCASQ
                     DVEAWGVVDNEHVRAFSAACYRRGLGARSIQRQLSSIRTFFRFMRREKHVKGNPAEGV
                     RAPKAGKRLPGNLDADRMARLLDLAGDGPIVARDRAILELLYSSGLRLSELTNLDVDH
                     IDVADATVRVTGKGGKERIVPVGKHALRALDEWRKERSMLAGPEERALFVSKRGGRIA
                     PRSVQARVDYWAKRQGIDTNVYPHLFRHSFATHLLESSHDLRGVQELLGHANISTTQV
                     YTHLDFQHLAQIYDKTHPRARRKSED"
     gene            complement(3324..4028)
                     /locus_tag="VFY27_10195"
     CDS             complement(3324..4028)
                     /locus_tag="VFY27_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX5765929.1"
                     /translation="MSTQRKAEFTEDVLSEADVHSYLQRHPDFFERHASLLSALRLPH
                     ATGAAVSLVERQVSVLRQKDLKLERKLKELLDVARANDALADKIHKTALDLLEARDPR
                     QTLGSVEAALRTEFGADHAVLVVFGEPASFGNSSPSRFFRPLERNDPALQPFATFMGS
                     SNPRCGQVRDAQRDFLFGEQTNEVGSAALVPLGDKCRVGFLAIGSVDADHFHPGMSFD
                     FLKRLGELIAVALKRH"
     gene            4354..4995
                     /locus_tag="VFY27_10200"
     CDS             4354..4995
                     /locus_tag="VFY27_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5765930.1"
                     /translation="MRHIMVMNAKGGCGKSTLATNIASYFADEGYSVALADYDPQRSG
                     LDWLERRPDSRPKIAGVSGFEDGMRRAPRNVDYMIIDAPARSHGTELTELVRHAETII
                     VPVLPSTIDIQASDRFIRELKSVGKVDRKEAKIGVVANRVRENTLIFEELDEYLTSAR
                     IPYLASLREAQNYVRAYTRGLGIFELPEYLAWPDWEQWEPMTKWLRSVRSQPS"
     gene            complement(5065..6261)
                     /locus_tag="VFY27_10205"
     CDS             complement(5065..6261)
                     /locus_tag="VFY27_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX5765931.1"
                     /translation="MKFGLLFVLALVASAFAAHFLLQDPGYVIINFRSYIVEMSVPVL
                     LAALLLLGFTAWLVVKIFKAPRKLGEAAGRYRSGRAGHRLTRGMIEVAEGNFARGEKL
                     LASAASGSDAPLLNYLQAARAAHLLGEDERRDRWLKAAYENTPEAASAVLLTQAELQL
                     DQGQYEQALATLRQLEEKAPNHSYALNLLGRLYYRLQDWTHLEALLPRLKKHGRVDDE
                     LLGKWAIRVHKEHLETAKDADAVLAAWNSVPKNLRNDGSVLEAYFATLIRTGQHDKAE
                     KEVAAELKREWRPPLVRLFGLINGSDPAKQLSRAEGWLRTHPDDADLLLAGARLCLKN
                     ELWGKARSYLETAISIRPTPEAYEEYGRLLNQLGESDAAADAYKAGLNLVASPPRVAI
                     PDLRTT"
     gene            complement(6258..>7398)
                     /locus_tag="VFY27_10210"
     CDS             complement(6258..>7398)
                     /locus_tag="VFY27_10210"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5765932.1"
                     /translation="PAPPPRRGVSSAVAWLALLLALLALAGSGYLAWQDRASAADEAG
                     DEAAIAALSGELDDAVTSLGSRQQAALAELQDAARQRTARINSLEREIQELNEQNESV
                     YPRLRNLEDAISSLQGVSAGVRETWMLAEAEYYMQIANAQLQLAGKPHIAALALEFAD
                     ERVRQMANPALADVRRALSQELQALRSMETADIEGMTLALANLAERTASLPLRQDVDV
                     PDEETGAIDPELTGFSRAVASVKSVFSDIVSVRRSDEPLTPLLAPEAAYFLRANLALT
                     LQAARLALLQGEQRVYEQSLDDAAKWLDDYYDAGSGAVASALETIEQLRGEGVDVTPP
                     DISESLRLLRQYRTLQGAASPAGDDPDEESDSETGDEAGTDEPAQ"
BASE COUNT         1261 a         2391 c         2272 g         1474 t
ORIGIN      
        1 gaaatgcagg cggatggcgt agtggccgat gggctcaacg ccggtcacca ctacgttctc
       61 cttgcccgtc tgcagcactt cctccccgct cccgtggcca cggacttcgg cggacggcga
      121 attcactcgc aggtactcga acggcaggtc gaaacggctg ccgtcttcga attccagcga
      181 aagtaccctc gagttctttc gcagccggat ttcaactggc ggtgtatcca tgttcaggct
      241 ccggtttccg cacgagtctt cctttaaagg atgtagcggc tcaggtcctc gtccctggaa
      301 agctcagcca ggtgatcgtc gacgtaatcg tcatcgatgc gcaccgtcgt accgcttttg
      361 tcggacgcct cgaaggacac ggtctccagc aggcgctcca tgaccgtgtg caggcgcctg
      421 gctccgatat tctcggtgtt ctcgttgacg tgatacgcca cttcggcaag gcggcgcaca
      481 ccgccgtcgg tgaactcgag ccgtacgtcc tccgtcccca gcagggcgct gtattgcgcg
      541 gtcagcgagg catccggctc ggtcaggata cgcacgaaat cctcggtctt tagcgatttg
      601 agctcgaccc ggatcgggaa acgtccctga agttccggaa tcaggtccga gggttttgac
      661 acgtgaaacg ctcctgatgc aatgaagagg atgtgatccg tacgcaccat cccgtacttc
      721 gtattgatcg tggagccttc caccagcggg agcaggtcgc gctgcacgcc ctcgcgcgag
      781 acgtccgcgc ccatggtctc cgatcgcctg gcgaccttgt ccagttcgtc caggaacacg
      841 attccctgct gttcgaccgc ctccagcgcc tgtgccttca gttcctcctc gtcgaccatc
      901 ttcgccgctt cctcgtccgt cagcagcttc atcgcttcct tcactttcag cttgcgagtc
      961 ttctggcggt tgccggagag attctggaac atgccctgca gctggctcgt catctcctcc
     1021 atgccggggg gcgccatgat ctcgaccccc atcggcatcg cctggagcgc aacctcgatc
     1081 tccctgtcgt ccagctggcc ctcgcgcagc atcttgcgga atttctggcg ggtatcgctt
     1141 tcgccgcgga gcaggcggtt gtcatcctcc ggcacgcggt tgcggggcgg tggcagcagg
     1201 gcatccagca cgcgctcctc cgccgcatct tcggcgcggt gccgtacttt ccccatagcc
     1261 tcctcgcgaa tcatcttgac ggcgacatcc atgagatccc ggatgatgct gtcgacctct
     1321 cgcccaacgt agccgatctc ggtgaacttg gttgcctcga ccttgatgaa aggagcgcgg
     1381 gcgagacgcg cgaggcgacg cgagatctcg gtcttgccga cgccggtcgg cccgatcatc
     1441 aggatgttct tcggcgtgat ttcgctgcgc aagggttcat ccacctgtac gcggcgccag
     1501 cggttccgca gcgcgatcgc cacggctcgc ttcgcttcat cctggccaat gatgtgcttg
     1561 tccagttcct ggacgatttc ccttggtgtc atcgttgaca ttggttaatc ctgtttcacc
     1621 tccgccgggg gcagttcctc gacgatgatg ttgtgatttg tatagacgca gatatctccc
     1681 gcaattccca aggccttctc gacgattgcc cgggcttcga gttcggtatt cctgacgagc
     1741 gcgagcgcgg ccgcctgggc gaagagcccg ccggagccga tcgccatgac gtcatgctcg
     1801 ggttctatga cgtcgccgtt cccgcttacg acaaaggaag actcggcatc cgcgacgcac
     1861 agcagcgctt ccaggcgccg cagccgccgg tccgttcgcc agtccttcgc cagctcgatt
     1921 gccgcgcgcg tcaggtttcc atagcgttcg agcttgcttt caaaaagctc gaacagtgtg
     1981 aacgcgtccg cggtgccacc ggcgaaaccg gctatgacgc ggccttccgc cagcgtccgc
     2041 accttgcggg cattgccctt catcaccgtg ttgcccatgc tcacctgccc gtcaccgccg
     2101 atggcgacac ggccatcgcg gcggacggag aggatcgtgg tcccgcgaaa ctgctccatt
     2161 cctgattcct taagccatgg attatcgaaa gtgtggtcgc cgcgactgtg aaaagcggcc
     2221 gccaccgtgc cggcagtcgc cggcgctccg ccctgtcagg cggtcacgaa cggatccggt
     2281 cagtcctcgg acttgcggcg cgcgcgcgga tgggtcttgt cgtatatctg ggccaggtgc
     2341 tggaagtcaa gatgggtata gacctgggtc gtgctgatgt tggcgtggcc gaggagttcc
     2401 tggacgccgc gaaggtcgtg gctggattcg aggaggtggg tggcgaagga atggcggaac
     2461 aggtggggat acacattcgt atcgataccc tgtctttttg cccagtaatc cacgcgcgcc
     2521 tgtaccgagc gtggcgctat ccttcctccg cgcttgctga cgaacaaggc gcgctcttcc
     2581 gggccggcga gcatgctgcg ctcttttcgc cactcgtcca gggcgcggag ggcgtgcttg
     2641 ccgactggga cgatgcgttc cttgccgccc ttgcccgtca cccggacggt tgcgtcggca
     2701 acatcgatgt ggtccacatc gagattcgtc agctccgaaa gacgcaagcc cgatgaatag
     2761 aggagttcca ggattgcgcg gtccctggcg acgatcggac catcgccggc gagatcgagg
     2821 agccgcgcca tgcgatccgc gtccaggttt ccgggcaggc gtttccccgc cttgggagcc
     2881 ctgacgccct cggccggatt tccctttacg tgcttctcgc ggcgcatgaa gcggaagaag
     2941 gtccggatgg atgacagctg tcgctgtatg cttcgcgccc ccagccctcg ccggtaacag
     3001 gctgcgctga aggcgcggac gtgctcgttg tcgactacgc cccaggcctc gacatcctgc
     3061 gaggcgcacc agaggcgaaa ggcttcgagg tcgcggccgt agtgctcgca cgtcagcttc
     3121 gagagccggc gctcgtagtc gaggtgccgg atgaatcgct cgatccactc tttttcgctg
     3181 tcaggcatgt cggcgtccga cgctttctct cccggtagga gcccgccacg cgggcgagtg
     3241 cggttcaggt tatcgaattc ccccgcatgg cgggctcctg cagtctctcc aagaccactt
     3301 caggtccccg cggtgagggt cccttagtgc cgcttgagcg ccaccgcgat aagttccccc
     3361 aggcgcttca ggaaatcgaa gctcattccc ggatgaaaat ggtcggcatc gacactgccg
     3421 atcgcgagaa agccgacccg gcacttgtct cccaaaggca cgagcgcggc agatcccact
     3481 tcattggttt gctcgccgaa caggaagtcc cgctgcgcat cgcgaacctg gccgcagcga
     3541 ggattgctgc tccccatgaa ggtggcaaac ggctgcaagg cgggatcgtt ccgttcaagt
     3601 ggccggaaga agcggctggg gctcgagttg ccgaaggagg ccggctcgcc gaacaccacg
     3661 agtacggcgt gatcggcccc gaactctgta cgcaacgccg cttcgacgga tcccagcgtc
     3721 tggcgagggt cgcgtgcttc gagcaggtcg agcgccgtct tgtggatctt gtccgcaagc
     3781 gcgtcgttgg cacgggcaac gtcgagcagt tccttcagtt tccgctcgag cttcaggtcc
     3841 ttttgccgca ggacggaaac ctgccgctcg accagcgaaa ccgccgcgcc agtagcgtgt
     3901 ggcaggcgca aagcgctgag gagcgaagcg tgacgctcga agaaatcggg atgtctttgc
     3961 aggtacgaat gcacgtcggc ttccgacaat acatcttcag tgaattcggc ttttcgctgc
     4021 gtgctcatgg atcgtggctc ccctcgtgcg caaatgtcac ttttgttctt tgttcgtttg
     4081 tatggcggcc cgccgtcctt ttccgtcatc agcagcctgt cggagcttac catgagtcgg
     4141 cctcggcact ccgacgaaaa gcacccccgg gactcgcgat aaacgttcga aactgcatcg
     4201 atggtgaatg ctgcacccca catgggcgat gcgcatgtat ggcgaattta ccgaacacta
     4261 tccgcgaatg gacttttggc gtccgcgacg gtagtttaca taaacggata acgctacaaa
     4321 caacgaaatc aataaacgct ttccggggga ataatgcggc acatcatggt tatgaacgcc
     4381 aagggaggct gcggcaagag cactcttgcg acgaatatcg cttcctattt cgcggacgag
     4441 ggatattcgg tcgcactggc cgattacgac ccgcaacgct cgggactgga ctggctggag
     4501 cggcggcctg acagtcgccc gaagatcgcc ggcgtatccg gcttcgaaga cggcatgagg
     4561 cgtgctccgc gcaacgtcga ttacatgatt atcgacgcgc cggcgcgatc gcacggcacg
     4621 gagctgaccg aactggttcg gcatgccgaa accatcatag tgccggtact cccgtcgaca
     4681 atcgacatcc aggcctccga ccggttcatc agggaactga agtcggtcgg caaagtggac
     4741 cggaaggagg ccaagatcgg cgtggtcgcc aaccgcgtgc gcgagaatac cctcattttc
     4801 gaggagctgg acgaatacct gacctctgcg cgaataccgt acctggcgtc gttgcgagag
     4861 gcccagaact acgtcagggc ctacacccgc gggctcggca tattcgagct ccccgagtac
     4921 ctggcatggc cggactggga gcagtgggag ccgatgacca agtggttgcg cagcgtccgc
     4981 agccaaccaa gctgacaaga gcgcaggagc cggccatgcg gcgagtgggc tgaacggcgg
     5041 cgcactcgcc cgcaaggcgg gctcctacgt agtgcgcagg tccgggatgg ctactcttgg
     5101 cgggctcgcg accaggttga ggcccgcctt gtaggcgtcc gctgctgcat cgctttcgcc
     5161 cagctggttc aacagccggc cgtattcttc gtaggcttcc ggagtcggcc ggatcgagat
     5221 ggccgtctcc aggtagcttc gcgccttacc ccatagttcg ttcttcaggc acaggcgggc
     5281 cccggccagc aacagatcgg cgtcgtcagg atgcgtgcga agccagcctt ctgcccggct
     5341 caactgcttt gccgggtcgc tgccgttaat gagcccgaag aggcgaacga gcggcgggcg
     5401 ccattcgcgt ttcagctccg cggcaacctc cttctccgcc ttgtcgtgct gccccgtcct
     5461 tatgagcgtg gcaaaatacg cttcgagaac ggaaccgtcg tttcgcaggt tcttcggcac
     5521 actgttccac gctgccagaa ccgcatcagc gtccttcgca gtttccaggt gctccttgtg
     5581 aacgcggatc gcccatttac caagcagttc gtcgtccacc cggccgtgct ttttcaggcg
     5641 tggcaacagg gcctcgagat gcgtccagtc ctgcaggcgg taataaaggc ggccgaggag
     5701 gttcagcgca taactgtggt tgggcgcctt ctcctccagc tggcgcaagg ttgcgagggc
     5761 ctgttcgtac tggccctggt cgagctgcag ttccgcctgg gtgagcagga ccgcactcgc
     5821 cgcctcgggc gtattctcat atgcggcttt gagccagcgg tcacgccgct catcctcgcc
     5881 gagcagatgc gccgctcgcg cggcctgcag gtagttgagc agtggcgcat cgctgccgct
     5941 ggccgcgctg gccagcaact tctcgccgcg tgcgaagttc ccttctgcaa cttcgatcat
     6001 gcccctggtc agcctatgcc cggcacggcc gctccggtag cggcctgcgg cttcgcccag
     6061 tttcctcggc gccttgaaga tcttcactac cagccacgcc gtaaaaccga gcagcaggag
     6121 cgcggccagc agcacgggca ccgacatttc cacgatgtaa ctgcgaaagt tgataatgac
     6181 gtaaccggga tcctgcaaca ggaaatgcgc ggcgaacgcg cttgctacca gcgccagcac
     6241 gaagagcagg ccgaatttca ttgcgcgggc tcgtccgtgc cggcctcgtc gccggtctcg
     6301 ctatcgcttt cctcgtcagg gtcatcgccg gcaggcgacg cggcgccctg cagcgtccgg
     6361 tactggcgca gcaagcgcag cgattccgat atatccggag gcgtcacgtc gacgccttcc
     6421 ccccgcaatt gctcgatggt ctcgagggca ctggcgaccg ctccgctgcc ggcatcgtag
     6481 taatcgtcca gccacttcgc cgcgtcgtcc agcgactgct cgtatacccg ctgctctccc
     6541 tgtagcaggg cgagtcgcgc cgcctgcagc gtaagtgcaa ggttagcccg gaggaagtag
     6601 gctgcctcag gggccagcag gggcgtcagc ggttcgtccg accttcgtac cgaaacgata
     6661 tcgctgaaca cgctctttac agaggcgacc gcacgcgaaa agccggtaag ctccgggtct
     6721 atcgctcctg tttcttcgtc cggcacatcc acatcctgcc tgaggggcag ggaagcagtg
     6781 cgctcggcga gatttgccag cgcaagcgtc atcccctcga tgtcggcggt ttccatggag
     6841 cgcagcgcct gcagttcctg cgacagggca cggcggacgt cggcaagcgc cgggttcgcc
     6901 atctggcgga cgcgctcgtc tgcgaactcg agggccagcg ctgcgatatg cggcttgccc
     6961 gcgagttgca actgggcgtt ggcgatttgc atgtagtact cggcctcggc gagcatccag
     7021 gtctcccgga caccggcaga gactccctgc aaggacgaga tcgcgtcttc gaggtttcgg
     7081 agccgcgggt acaccgactc gttctgctcg ttgagttcct ggatttcccg ctcgagcgag
     7141 ttgatcctcg cggttcgctg ccgcgccgcg tcctgcagtt cggcgagcgc cgcctgctgc
     7201 cgcgacccga gtgaggtcac cgcgtcgtcc agttcacctg acagcgcggc gatcgctgcc
     7261 tcgtcaccgg cctcatccgc ggcgctcgcc cgatcctgcc acgcgaggta accgctcccc
     7321 gcgagcgcca gcagtgcgag caaaagcgcg agccacgcga cggcggagga aacgcctcgc
     7381 cgcggcggtg gggcaggc
//
LOCUS       DASUOM010000489         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1260739,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000489 DASUOM010000000
VERSION     DASUOM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260739"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..>2942)
                     /locus_tag="VFY27_10215"
     CDS             complement(9..>2942)
                     /locus_tag="VFY27_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765933.1"
                     /translation="LSVGAVLSWHFLSKAEKGMIGELRNRAESLTTNLARSSKWGLLS
                     EDEVILDEIVSDILREDGVLYVAISNSLGEILASRVRSGERSDPLDIAILHAEAMAKT
                     NDSAAIHFHEIDGTGTYHTYMPVESTPAFRSQTDEEISRALSLFGDTPAAAGSDSGDR
                     YGNVQLLVSSKKMFDEIQNSFITGIGLTLLIVLLALAVSYLFCSRVLGPVKAMAAAAR
                     SISSGDLSQRIKVRSHDEIGVLAETFNHMSSSLSRMTRIQAERLDALSALHEAGLLIN
                     STLDVEKVIELTLDIVVRRLGYSRALLFLTDWDRGVLTRGRIVGASEDIKRRFRDLEI
                     PLDERGGFCARAAASGQSVLVSDINKLQGKANATILSLLGPNAFLAVPLMLKDRSLGV
                     MAIECRPGHELGSADTALMETLANQMAIAIANAISFQEIEQLNINLEAKVRERTGELR
                     QQQARLEEVNEELVKATRHKSEFLANMSHELRTPLNAVIGYSELLQEEMEEQNLSEYI
                     ADLGKINSAGKHLLALINDVLDLSKIEAGKMDVYMEDGDIAEIVDEVRTTIKPFVESY
                     GNRFTVTCPPGRIRTDITKLRQILFNLLSNAAKFTENGAVSLDVTKTQRDGVDEWQFR
                     IADTGIGIAPDKMDRLFEEFSQLDAEVARKHGGTGLGLAISQRFCRMLGGEITVTSQP
                     GHGSTFTVALPAPEGKPAAQKVQAAAGKAVPAKSHKAGSEHCDVLVVDDDPAVRDLMA
                     RYLVKEGFRVRTASTGEEGLELARRLHPDAVTLDLLMPGLDGWSVLTQLKADDKLADI
                     PVVIVTILDNKDMGYTLGAADYLTKPVDPQRLVSIMRQHCSKQTNAPIMIIEDDADSR
                     RLLSSMLVKQGWTVIEAEDAEAGLAGLARQEPALILLDLILPGMDGFEFIEAVHKNKG
                     WSKIPIVVVTAKDLTPKEHEFLNRSVQTIVRKGVYSRGELLDVVHSTMLDAVSQKEDT
                     AENLS"
BASE COUNT          524 a          910 c          866 g          642 t
ORIGIN      
        1 ctcgccggtc aggaaaggtt ttctgccgta tcttcctttt gtgagaccgc atccagcatc
       61 gtggaatgga cgacgtcgag cagttcgcca cggctgtaaa cacctttcct gacgatcgtc
      121 tggacgcttc gattcaggaa ctcgtgttct ttcggagtca ggtccttcgc cgtaaccaca
      181 acgattggaa tcttgctcca gcccttgttc ttgtgcacgg cctcgatgaa ttcaaagccg
      241 tccatcccgg gcagaatcag gtcaagcagg atgagcgcgg gctcctgccg ggcaaggccc
      301 gcaaggccgg cctccgcgtc ttccgcttcg atcacggtcc atccctgttt caccagcatg
      361 ctgctcagca ggcgccggct gtcggcatca tcctctatta tcatgatcgg cgcgttcgtc
      421 tgcttgctgc agtgttgccg catgatcgat acgagccgtt gcggatcgac gggcttggtg
      481 aggtagtctg ccgcacccag ggtgtagccc atgtccttgt tgtccagtat ggtgacgatg
      541 acaacgggaa tgtcggccag cttgtcgtct gctttcaact gcgtcagtac cgaccagcca
      601 tcgagaccgg gcatcagcag gtcgagagtg acagcgtcgg gatgtaaacg ccgcgccagc
      661 tcgagccctt cctctccggt cgatgccgtt cgaacgcgga agccttcctt aaccaggtag
      721 cgcgccatca ggtcacgcac tgccggatcg tcatcgacaa ccagcacatc gcaatgctcg
      781 ctgccggcct tgtgcgattt cgccggcact gccttccctg ccgcggcctg gaccttctgc
      841 gcggccggct ttccctcggg cgccggaagt gcaacggtaa acgtggaccc gtgcccgggc
      901 tggctggtga ccgtgatctc gccgccgagc atccggcaga atcgctggct tatcgcaagg
      961 cccagcccgg tgccgccgtg cttccgcgcc acttccgcat cgagctgtga gaattcctcg
     1021 aagagcctgt ccatcttgtc gggcgcgatg cctatgccgg tatcggcaat gcggaactgc
     1081 cactcatcga caccgtctct ctgcgtcttc gtgacgtcca gcgataccgc accgttctcc
     1141 gtaaacttgg ctgcgttact gaggagattg aagaggatct gccgcagctt ggtgatgtcg
     1201 gtgcggatcc ggcccggtgg acaggtcacg gtgaagcgat tcccgtacga ctcgacaaaa
     1261 ggcttgatcg tcgtgcgcac ctcgtccacg atttccgcga tgtccccatc ttccatgtat
     1321 acgtccatct tgcccgcttc gatcttcgag aggtcgagca catcgttgat cagggcgagc
     1381 agatgcttgc cggccgagtt gatcttgccg aggtcggcta tgtattcaga taggttctgc
     1441 tcctccattt cttcctgcag caattcgctg tagccgataa cggcgttcag gggcgttcgc
     1501 agttcgtggg acatgttggc gaggaattcg gacttgtgcc gggtcgcctt caccagttcc
     1561 tcgttaacct cctccaggcg ggcctgctgc tgccgtagtt caccggtcct ctccctgacc
     1621 ttcgcctcga ggttgatatt cagctgctcg atctcctgga agctgatggc gttggcaatg
     1681 gcgatcgcca tctggttggc aagggtttcc atcagtgcag tgtcggcgga tccgagttca
     1741 tggccggggc ggcactctat tgccatcacg cccagcgacc ggtccttcaa catcaggggc
     1801 acagcaagga acgcgttcgg cccgaggaga ctcaggatcg tggcgttcgc cttcccctga
     1861 agcttgttga tatcgctaac gagcacggat tgaccgctgg ccgcggcgcg tgcgcaaaac
     1921 ccgccacgct cgtcgagcgg gatttcaagg tcgcggaagc gccttttgat gtcttcgctc
     1981 gcgcctacga tcctgccgcg ggtaaggacg ccgcgatccc agtcggtaag gaacagcaaa
     2041 gcccggctgt agccgagcct cctgaccacg atgtccaggg taagctcgat taccttttcc
     2101 acgtcgagtg tggaattgat cagcaagccc gcctcgtgca gcgccgacaa cgcatccagg
     2161 cgctccgcct ggatgcgggt cattcgcgac agcgaactgc tcatgtggtt gaaggtctcg
     2221 gcgagaacgc cgatctcgtc gtgtgagcgg accttgatcc gttgcgacag gtcgcccgac
     2281 gagatactcc gggccgcggc ggccatggcc ttgacgggtc cgaggacgcg cgaacagaag
     2341 aggtaggaga cggcgagcgc cagcagcacg atcagcagcg tcaggccgat accggtgatg
     2401 aacgagttct ggatttcgtc gaacatcttt ttcgatgaaa ccagcaactg cacattgccg
     2461 taacgatcgc ccgagtcgga acctgccgcg gcaggcgtat ccccgaacag cgacagcgcc
     2521 cgggagattt cttcgtcggt ctggctgcgg aaagcaggag tggattcgac gggcatgtac
     2581 gtatggtagg tgcctgtccc gtctatctcg tgaaagtgaa tggctgccga atcgttcgtc
     2641 ttggccatcg cttcggcatg caggatggca atgtccagcg gatccgatct ctcgccgctc
     2701 cgcaccctcg atgcgagtat ttcgccgagg ctattgctga tcgcgacata cagaacgccg
     2761 tcttcgcgaa ggatgtcgct cacgatctcg tcgaggatca cctcgtcctc gctgagaagg
     2821 ccccatttgc tgctgcgggc gaggttcgtc gtcagcgatt cagcgcggtt gcgcaactcg
     2881 ccgatcatcc ccttctccgc tttgctcaaa aaatgccagc tcagcactgc acccacggag
     2941 ag
//
LOCUS       DASUOM010000490         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_422839,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000490 DASUOM010000000
VERSION     DASUOM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422839"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFY27_10220"
     CDS             <1..258
                     /locus_tag="VFY27_10220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5765934.1"
                     /translation="LHDDGTTGEYYHLQQNGALVEPGDRVEAGQMIGLSGNTGHTTVP
                     HLHFAVYRAAEWGTTQSIPVRFASADGIIEKPRRGARYPAT"
     gene            328..933
                     /gene="folE"
                     /locus_tag="VFY27_10225"
     CDS             328..933
                     /gene="folE"
                     /locus_tag="VFY27_10225"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5765935.1"
                     /translation="MTSKNKNGKQGARPTRKEAEEAVKTLLLWAGEDPAREGLVDTPR
                     RVAKAYEDWFGGYDLDPISFLERTFEEVDGYDEMIVLRDITFESHCEHHMAPIIGKAH
                     VGYLPNNKIVGISKLARVVDAFARRFQVQEKLTAQIAQCIQSVLKPKGVGVVVDAVHQ
                     CMTTRGIHKAGVSMVTSQMLGSFRNDARTRAEFLGVIGKGS"
     gene            complement(953..>2098)
                     /locus_tag="VFY27_10230"
     CDS             complement(953..>2098)
                     /locus_tag="VFY27_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5765936.1"
                     /translation="AYADELGRAASLLRDAADLAEHEGFANYLRLRAEALVTDNYQPS
                     DLAWMDVKTNPIELVIGAIETYEDRLFGYKAAYESYVLLKDQEWSERLARFAGFLPEL
                     QEGLPVPPEFKAETPGLDSDVNAYDVLYYAGHSNAGSKTIAINLPNDEQVQLQKGTRR
                     LQLKNAMQAKFEKILVPIAGVLISASQRQHISFDSFFTNTMFHEVAHGLGIKNTINDK
                     GTVREALKDLASSMEEGKADVLGLYMITSLHEAGELGEVDLRDNYVTFMAGMLRSMRF
                     GASSAHGKANMVRFNFFAERGAFVRDPETETYSVDFERMEEAMNALSGLLLTLQGTGD
                     YEGTAKLMNEKGSIDAALQSDLDKLMRADIPVDIEFSQGLNELGLAE"
BASE COUNT          417 a          643 c          627 g          411 t
ORIGIN      
        1 ctgcacgacg acggcacgac cggcgagtac tatcatctgc agcaaaacgg cgcgctggtg
       61 gaacccggcg accgcgtcga ggcaggacag atgatcggcc tgtcgggcaa tacgggccat
      121 acgaccgtgc ctcacctgca ctttgccgtg tatcgtgcgg cggaatgggg cactacccag
      181 tcgatacccg tgcgcttcgc aagcgctgac gggataatag agaagccgcg acgcggcgcc
      241 cgctaccctg cgacctgatc aggtcgcagc gcaaccggtg cggtaccgtt cgatcagtca
      301 accgagcgac agcaacaggg agtacctgtg accagcaaga acaagaacgg caaacagggg
      361 gcaagaccga cccggaagga agccgaggaa gccgtcaaga cgctgctcct ctgggcaggt
      421 gaggatccgg cccgcgaagg gctggtcgac acgccacgga gagtcgcgaa ggcgtacgag
      481 gactggttcg gcggctatga cctcgacccg atctcgttcc tcgagcgtac gttcgaggag
      541 gtagacggtt acgacgagat gatcgtgctg cgggacatca ctttcgagtc acactgcgag
      601 caccatatgg cgcccatcat cggcaaggca catgttggct acctgccgaa caacaagatc
      661 gtcggcatca gcaagctggc acgcgtggtc gacgcattcg cgcggcgttt ccaggtccag
      721 gaaaaactga cggcgcagat tgcacaatgc atccagagcg tgctgaagcc gaaaggtgtc
      781 ggcgtcgttg tcgacgccgt gcaccagtgc atgaccacac ggggaattca caaggcggga
      841 gtgtccatgg taacgagcca gatgctgggg tcattccgca acgatgcccg cacgcgtgcc
      901 gagttcctcg gcgtgattgg caaagggagc tgacgcccga tcaggctcac gcttattcgg
      961 ccagtcctag ctcgttcaat ccctggctga attcgatatc gacaggaata tcggcccgca
     1021 tcagcttgtc gaggtcgctt tgcagcgcgg cgtcgatcga gcccttctcg ttcatcagct
     1081 tcgccgtgcc ctcgtagtcg ccggtcccct gcagcgttag caagagtccg gacagcgcgt
     1141 tcatggcttc ttccatgcgt tcgaagtcga cactgtaggt ttccgtttcc ggatcgcgga
     1201 cgaaagcacc ccgctcggcg aaaaagttga aacgcaccat gtttgccttg ccatgcgcgc
     1261 tgctggcgcc aaaacgcatg gaccgcagca tgccggccat gaaggtcacg taattgtcgc
     1321 gcaaatcgac ctctcccagt tcgccggctt catgcaggct tgtgatcatg tagaggccga
     1381 gaacgtccgc tttgccttct tccatgctcg acgcgagatc cttgagcgcc tctcgcaccg
     1441 tgcccttgtc attgatcgtg ttcttgatac caaggccgtg ggccacttcg tggaacatcg
     1501 tgttcgtaaa gaaggaatcg aacgagatat gctgtctctg cgacgcggag atcagcaccc
     1561 cggcgatagg caccagtatt ttctcgaact tcgcctgcat cgcattcttc agctgcagcc
     1621 tccgggtgcc cttctgcagc tgcacctgct catcgttcgg caggtttatg gcgatggtct
     1681 tcgagccggc gttgctatga cctgcatagt agaggacgtc gtaagcattg acatccgaat
     1741 ccagtcccgg cgtctccgct ttgaactccg gcggcaccgg caggccttct tgcagctccg
     1801 gcaggaagcc tgcaaagcgg gccagccgct cgctccattc ctggtccttc aacagcacgt
     1861 aggattcata cgcggccttg tagccgaaca accggtcctc atacgtttcg atggcgccga
     1921 tgaccagttc gatcgggttg gtcttgacgt ccatccacgc caggtcgctc ggctggtagt
     1981 tgtcggtcac cagggcttcg gcccgtagtc tcaggtagtt ggcgaagccc tcgtgctccg
     2041 ccaggtccgc cgcgtcacgt agcagcgacg ctgcgcgacc gagttcatcg gcataggc
//
LOCUS       DASUOM010000491         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1022940,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000491 DASUOM010000000
VERSION     DASUOM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022940"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFY27_10235"
     CDS             <1..748
                     /locus_tag="VFY27_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5765937.1"
                     /translation="ARYGAKGLAYIKVNDASTGRDGLQSPILKFLPDSAVSGIMERTQ
                     AATGDLVFFGADKARVVNDALGALRVRLGEDRGMVAQGWRPLWVVEFPMFERDRQAQR
                     WTSLHHPFTAPSVDDPSALRESPAEVLSRAYDMVLNDSELGGGSIRIHSKTMQSAVFD
                     LLEITRDEAEEKFGFLLRALEFGCPPHGGIAFGLDRLVMLMAGAESIRDVIAFPKTQT
                     ASCPLTNAPGEVGAAQLREVGIRVRKPAAE"
     gene            754..1224
                     /gene="nudB"
                     /locus_tag="VFY27_10240"
     CDS             754..1224
                     /gene="nudB"
                     /locus_tag="VFY27_10240"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF006961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX5765938.1"
                     /translation="MATVPGARPSLAYRRPVSVLVLVYTPDGDVLLLRRSHPADIWQS
                     VTGSLQRDETHAEAARRELAEETGFLNGSGGSLTFGGNSRVFTIDPRWRDRYAPGITE
                     NVEFEWHFRLPAKTKIVMDPSEHTEYQWFPLDRAIEAVWSWTNREALVELRDRL"
     gene            1221..1868
                     /locus_tag="VFY27_10245"
     CDS             1221..1868
                     /locus_tag="VFY27_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5765939.1"
                     /translation="MTRDVVFVHGIWMPGAVMARLKQYLEHDHGFRGHLFSYASIRGT
                     LDENAELLADFIRDLGIDEAHLVGHSLGGVVSLRMLALNDDAPPGRVVCLGSPLCGCR
                     AATTISQHSWGSSVLGKALVAGVLDATASEWAASLTSKREIGIIAGTRPLGLGRLVTT
                     FDEDSDGTVTVAETRLPGAKAHLCMPVTHMGMTLSHEVANQVAAFLQRGEFPLRG"
     gene            complement(1942..2475)
                     /locus_tag="VFY27_10250"
     CDS             complement(1942..2475)
                     /locus_tag="VFY27_10250"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5765940.1"
                     /translation="MHLKRFRVLTMIPGVLVAILLLCPEPAVSFDERRLSFYHTHTAK
                     KLNVVYARGGEYVESALDDIESFLSDFRTGDARTIDPALLDLIYEIRESLGSNGTFEV
                     ISAYRSPQTNEMLRDRSSGVAKNSQHTIGKAIDIRLQGIDTAKLRDAAIARQGGGVGY
                     YQESNFVHVDTGRVRRW"
BASE COUNT          474 a          771 c          810 g          493 t
ORIGIN      
        1 cgcgcgctac ggcgccaagg gactcgcata catcaaggtg aacgacgcta gtaccggccg
       61 cgatggcttg caatcgccga tcctgaagtt tcttcctgat tccgccgttt ccggaatcat
      121 ggagcgcacg caggccgcca cgggagacct ggttttcttc ggcgccgata aggctcgcgt
      181 cgtcaacgac gccctgggcg cgttgcgggt caggctgggc gaagatcgcg gcatggtggc
      241 gcaaggctgg cgtcccctgt gggttgtcga attcccgatg ttcgagcggg atcgccaggc
      301 acagcgctgg acatcgttgc accacccgtt taccgcacct tcggttgacg acccgtcagc
      361 actgcgggaa tcacccgcgg aggtgctatc gcgagcttat gacatggtgc tgaacgattc
      421 ggaactcggc ggaggctcta ttcgtattca cagcaagacg atgcagtcgg cggtgttcga
      481 tctgctcgaa atcaccaggg acgaggctga agaaaagttc ggcttcctgc tgcgggctct
      541 cgagttcggc tgcccgccgc acggtggcat cgcgttcggc ctggaccgcc tggtaatgct
      601 gatggcgggt gccgaaagca ttcgcgacgt catcgccttt cccaagacgc aaacggcgag
      661 ttgtccgctc acgaacgctc ctggagaagt aggcgcggcg cagctacgcg aagtcggcat
      721 tcgcgtacgg aagcccgcgg cggagtaaag cctgtggcca ccgttccggg cgctcgtcct
      781 tccctcgcat accggcggcc ggtgtcggtg ctggtgctgg tgtacacgcc tgacggagat
      841 gtcctgttgc tgcggcgatc ccacccggct gatatttggc agtccgttac cggcagcctg
      901 cagcgcgacg aaacgcatgc ggaagccgct cggcgtgagc tcgccgaaga aacgggattc
      961 ttgaacgggt cgggcggaag tctcacgttc ggcggcaaca gccgtgtatt cacgatcgac
     1021 ccgcggtggc gcgaccgcta tgcgcccgga atcaccgaga acgtcgaatt cgaatggcat
     1081 ttccggctgc cggcaaagac gaagatcgtg atggacccgt ccgaacatac ggagtaccaa
     1141 tggtttcccc tcgaccgcgc aatcgaggcg gtgtggtcgt ggaccaaccg cgaagcgctg
     1201 gtggagctga gggatcgcct ttgaccaggg acgtggtgtt cgtgcacggc atctggatgc
     1261 cgggggcggt catggcgcgg ctgaaacagt acctggaaca cgaccacggt tttcgcgggc
     1321 acctgttcag ctacgcttcc attcgcggca cgctcgatga gaacgccgag cttctcgccg
     1381 atttcatccg cgacctcggc attgacgaag cgcatctcgt cgggcatagc ctcggcggag
     1441 tcgtaagcct tcggatgctt gcgttgaacg atgatgcgcc gccggggcgg gtcgtgtgtc
     1501 tcgggtcgcc gctgtgcggc tgccgcgccg cgacaactat cagccagcac agctggggca
     1561 gctccgtcct gggcaaggcc ctcgttgccg gcgtgctgga cgcaacggcc agcgagtggg
     1621 cggcttccct gacatcgaag cgggagatcg ggatcatcgc cggcacgcga cctttgggcc
     1681 ttggcaggct cgtgactacg ttcgacgagg acagcgacgg taccgtgaca gtcgcggaga
     1741 cccggctgcc aggtgcgaag gctcacctgt gcatgccagt gactcacatg ggaatgaccc
     1801 tgtcgcacga agtcgcgaac caggtcgcgg cgtttctgca gcgcggggag ttccctttac
     1861 gcggttgaga tgtttcgctc agctcgtcct gaaagcgcct aaaccggcgt cacgaatccg
     1921 gcgccaaccg gacctccaaa gctaccagcg acgcaccctt ccggtatcaa cgtggacgaa
     1981 attcgattcc tggtaataac cgacgccgcc gccttgcctg gcgatcgcag catcccgcaa
     2041 ttttgccgtg tcgattccct gcaggcgaat atcgattgcc ttcccgatcg tgtgctggct
     2101 gttcttcgcg acgccgctgc tgcggtcgcg gagcatctcg ttggtttgcg gtgagcggta
     2161 tgccgaaatg acctcaaacg tcccgttgct gccgagggat tcccggatct cgtagatgag
     2221 gtcgagcaat gcgggatcaa tcgtcctggc gtctcccgta cggaagtccg aaaggaaact
     2281 ttcgatgtcg tccagcgccg actcgacgta ctcaccccca cgcgcataca cgacattcaa
     2341 cttcttcgca gtgtgcgtgt gataaaagga aagcctcctc tcgtcgaagc tgacagcggg
     2401 ctccggacat aacagcagga tggcgaccag aacgccgggt atcatcgtca ggacgcggaa
     2461 ccgtttcaaa tgcatcagtt tgtccacagg ccggaggata gcttgattag tacaccggaa
     2521 acaggagtgg atcaagtttc ccgtcgca
//
LOCUS       DASUOM010000492         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_59049,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000492 DASUOM010000000
VERSION     DASUOM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59049"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFY27_10255"
     CDS             complement(<1..302)
                     /locus_tag="VFY27_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765941.1"
                     /translation="MLHEFLTLHRIELMERCKLKAALRFAPRKAAIPPDGVPLILDQL
                     IETLRAEYVTAMPNNMQIGESAPSKPRPLRTGQGLPAGDAEEMFRPGTQKGHDKIGL"
     gene            complement(471..546)
                     /locus_tag="VFY27_10260"
     tRNA            complement(471..546)
                     /locus_tag="VFY27_10260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Thr,seq:cgt)
     gene            complement(602..1012)
                     /locus_tag="VFY27_10265"
     CDS             complement(602..1012)
                     /locus_tag="VFY27_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5765942.1"
                     /translation="MSRADILKKLEQHRVTATPQRLEVAEVLLERPQHLSADQILERL
                     RVAGSRVSKATVYNTLKLFSERGLVRELTVDPDRRYYDSTTHAHHHFFHVGTGELSDI
                     PEDQVEILKLPALPAGTEQESVEVLIRVRDRQAP"
     gene            1194..1733
                     /locus_tag="VFY27_10270"
     CDS             1194..1733
                     /locus_tag="VFY27_10270"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5765943.1"
                     /translation="MRRLPGAGGYSLYELLMTLALAGLMLTLGLPSFGSLIADKRLRV
                     ESDALFHAVHLARKGSIVRRRVVSLCPSVDGESCSPGYDWSSGWIVFANVDRDEPPQV
                     DENEPIISRHSVDGSVQLLANRRGFTLRSTELRATNGTFVICDRAARATPRALVVSYT
                     GRPRVARSDSRGNAYKCTD"
     gene            1833..2423
                     /locus_tag="VFY27_10275"
     CDS             1833..2423
                     /locus_tag="VFY27_10275"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5765944.1"
                     /translation="MNRQKQHGFTLYELLITLLVIGIVLVIGVPNLGDFTRNSRITAT
                     ANELHSSFMVARSEAARAKSNITICASASPLSAAADCDGASFNEGWIIFVDLNGDITR
                     AGVDENLLRAHEPVDDNIDILTGNGATYFSFAANGLGRGDVNGPSFATATICDERGHQ
                     VAAGGNSAARRLVVTPVGRSIVIRDFDLIDAAGGCP"
     gene            2461..>2892
                     /gene="pilV"
                     /locus_tag="VFY27_10280"
     CDS             2461..>2892
                     /gene="pilV"
                     /locus_tag="VFY27_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEX5765945.1"
                     /translation="MHTKNMKPTFRVACLWGFSLIEVLIALIIMSVGMLGIAGLYVHS
                     MQAGRTSLFRHHAVTLAGDVADRIRANPRAGVAYEDGGPGAGTEEYNCVAGGGDCSPA
                     EMAAHDMSLWDQQAVATLPAGDVLVDYNNGAVPSVYTVTVTW"
BASE COUNT          525 a          874 c          888 g          605 t
ORIGIN      
        1 agtccaattt tgtcgtggcc tttttgagtg cctggccgga acatctcctc cgcgtctcct
       61 gccggcagtc cttggccggt tcttagcggt cgtggtttgc tcggggcgga ctcaccgatt
      121 tgcatgttgt tgggcatcgc cgtcacatac tcagcgcgca gcgtttcgat cagttggtcg
      181 aggatgaggg gtacaccgtc cggtgggatc gctgctttcc gaggcgcgaa tctgagagcg
      241 gccttcaact tgcagcgctc catcaattca atccggtgca gtgtaagaaa ttcgtgcagc
      301 atcgccgttt ccctttgccg gctccgcgcc agtccataca gtacgctttc tgttggctct
      361 cgcggcgcct cgtgcgccgt cgctcgagaa ctatgggaat gttgccaccg tccgcgggca
      421 agatcggtgc ggtggcgaac agaaggtgtc cgggatggac cgacggttgt tggtgccgaa
      481 gagaggattc gaacctccga cccacgcatt acgaatgcgt tgctctacca actgagctac
      541 ttcggcagcg gcgcgagtat agccttgcgg ccgcgacgcg gccagcgcag gactgtccga
      601 tttaaggggc ttgcctgtcc cgtacgcgaa tgaggacttc gacgctttcc tgctccgtcc
      661 ctgccggcaa cgcgggcagc ttcaggatct ccacctggtc ctccggaatg tccgaaagtt
      721 cgcccgtacc gacatggaag aagtggtggt gagcgtgagt ggttgaatcg taatacctgc
      781 ggtccgggtc caccgtcagc tccctgacca gtccccgttc gctgaaaagc ttcagcgtgt
      841 tgtacacggt cgccttggac acacgactgc cggccacgcg caggcgttcg aggatctggt
      901 cggccgacag atgctgcggc cgctcgagca acacttccgc cacttcgagg cgttgcggcg
      961 tcgcggtaac ccggtgttgc tccagctttt tgaggatgtc cgctcgggac atggcgcaaa
     1021 tcctgaaatt tggaaagtat cagaaggttg cgagtataga ccaatcgagc tcgggaaatc
     1081 tccacccggc agcccggatt tcgcagtaaa tcgggacaaa cagctgggaa atcgcgctgg
     1141 tctccgttcg cgccgccgac ctaacctgct cccgtcgaac aaggaggttt gatatgcggc
     1201 gactgccagg agcggggggt tacagcctgt acgaactgct catgacgctt gcgctggcag
     1261 ggctgatgct gacgctcggc ctgccctcct tcggctccct gatcgccgac aagcgcctgc
     1321 gggtggaatc ggacgcgctt ttccacgcgg tgcacctggc gagaaaagga tcgattgtcc
     1381 gccggcgcgt ggtgtctcta tgtcccagcg tcgatggtga atcctgcagc cccggttatg
     1441 actggtcgag cggctggatc gtcttcgcca acgtggatcg tgacgagccg ccgcaggtcg
     1501 acgaaaacga gccgatcata agccgtcatt ccgtagacgg ctccgtgcag ctcctcgcca
     1561 atcgccgcgg gttcacactc cgctccaccg aattgcgggc gacgaacggc acctttgtca
     1621 tctgcgaccg cgccgcgcgg gcgacgccgc gggcactggt cgtcagctat accggcaggc
     1681 cccgcgttgc ccgttccgac agccgtggca acgcctataa atgcacagat taagttaaac
     1741 cattatgtgg aagttcgacc gcttgtcggg gcacggctgc cgctcgtcgg aaataaacag
     1801 gtgtggagaa agccggtgcg tatattccgc gcatgaaccg acaaaagcag cacggcttta
     1861 cgctctacga actgctcatt acattgctgg tcatcggcat cgtcctggtg atcggcgtgc
     1921 ctaacctggg cgacttcacg cgcaacagtc gtatcacggc gaccgccaac gagctgcatt
     1981 cgagcttcat ggtggccagg tcggaagcag cacgggccaa gagcaacatt acgatttgtg
     2041 ccagtgccag tccattgtcc gccgctgccg attgcgacgg cgcttccttc aacgaaggct
     2101 ggattatctt cgtcgacctg aacggagata ttacccgcgc gggcgttgac gagaacctgt
     2161 tgcgcgcaca cgaaccggtt gacgataaca tcgacatcct caccggcaat ggcgcgacct
     2221 atttcagctt tgcagccaac ggtcttggtc gtggtgacgt caatggcccg tctttcgcga
     2281 cggccacgat ctgcgacgag cgtgggcacc aggtcgcggc cggtggtaac tcggccgcgc
     2341 gacgcctggt cgttaccccg gttggcaggt caatcgtcat ccgcgacttc gatctcatag
     2401 atgccgcagg cggctgcccc tgacggccgc ctgggattat atttcaggaa gcttgagtac
     2461 atgcacacga aaaacatgaa gcccacgttc cgggttgcct gcttgtgggg cttttcactt
     2521 attgaagtgc tgattgccct gatcatcatg tccgtcggca tgctcggtat cgcgggtctc
     2581 tacgtgcaca gcatgcaggc cggtcgcacg tccctcttcc gtcaccacgc ggtcacgctg
     2641 gctggcgacg ttgccgatcg tatccgggcc aatccccggg cgggggtggc gtatgaggat
     2701 ggaggtcccg gtgcgggtac cgaggaatac aactgtgtgg cgggcggcgg cgactgctca
     2761 ccggcggaga tggcagcaca cgacatgtcg ctgtgggacc agcaggcggt tgcaacgctg
     2821 ccggccggag atgtcctcgt cgattacaac aacggcgccg taccctctgt gtacaccgtt
     2881 accgtcacgt gg
//
LOCUS       DASUOM010000493         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_799713,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000493 DASUOM010000000
VERSION     DASUOM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799713"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..469
                     /gene="hisE"
                     /locus_tag="VFY27_10285"
     CDS             125..469
                     /gene="hisE"
                     /locus_tag="VFY27_10285"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX5765946.1"
                     /translation="MTTHSDSKPDSIRFLASLDELIRERMAARPADSYVASLVASGDK
                     RLAQKLGEEAVELAIAVTAGDRDEQLGEAADLLFHMLVALNAKGVRLEHVSAVLEQRH
                     AKMALQKSEETQ"
     gene            complement(599..3238)
                     /locus_tag="VFY27_10290"
     CDS             complement(599..3238)
                     /locus_tag="VFY27_10290"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HEX5765947.1"
                     /translation="MLHSQVEEAPFSDALGASEAIRKSDWVVMKFGGTSVSTADNWRT
                     IAALIQNRRAQNLRILIVHSALRGISNELADALDAAVSGDPAANIAAIREQHYQLADD
                     LGLDRHALLDPTLQELDQLLAGVRLIQEVSSRVRARVMAMGELMSTTLGAAWLNRCGI
                     DCELRDAREMLSTEDGAGRSEAQNYLSATCSYSADEGLQASLGTSGKVVLTQGFIASN
                     SRGATVLLGREGSDTSAAYFAAMLQARRLEIWTDVPGMYTADPKLVPSARLLVELHFD
                     EAQELASMGSKVLHPRCLSPLRAHGIPLFIRCTTAPHIPGTVVSAVTLEVEPQVKGIS
                     TRSGTVLISMEGSAMWHEVGFLAEAFAVFRRHGVSVDLVSTSQTNVTVSIDTADEMFS
                     EEIRMALMRDLESLCQARLIPDCAVVSLVGRKIRTILPRLAPALSSFEEEKIHLVSQA
                     SNDLNFSFVIDQVQVGRLVSKLHSSMIGQSSSSTAFGPTWEELFRERPAPLPREDAWW
                     HRKRSRLLEIAEAEANAYVYDLESVTAAATDLLQMKSLDRVLYAMKANFNPHILQTLA
                     GLGVDFDCVSPGEVEHLRKVIPGIEGRRILLTLNFAPRNEYEWAAENDLLLTLDNLHP
                     LREWPEIFRNREIFIRLDPGHGRGHHAHVRTAGVHSKFGIPRFELDELIRLVDEAGAR
                     VVGIHAHSGSGILDPENWRTVAGELIKVARRFPHVRALDLGGGLGIPERPGDRQFDLR
                     QLDAALIEIRKACPDYELWLEPGRFLVARAGVLLTHVTQTKGKGDMHYIGVGTGMNSL
                     IRPALYGAYHEIVNLSRLDEPATDTVTVVGPICETGDRLGIDRLLPASREGDVILVLN
                     TGAYGYVMGSNYNMRGIAKEVLI"
     gene            complement(3246..>3874)
                     /locus_tag="VFY27_10295"
     CDS             complement(3246..>3874)
                     /locus_tag="VFY27_10295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5765948.1"
                     /translation="GQEVVHSFDRPIKADSHLVILYGNLAPEGAVAKITGKEGLRFSG
                     RARVFHSEELALRAILDDVVKAGDVVVIRYEGPKGGPGMREMLSPTGAIIGKGLGADV
                     ALITDGRFSGGSHGLVVGHVSPEAAVGGPLALVEDGDTITIDAAARRVDLDVTDEMLV
                     ERRRHWRRPPSSVTGGVLAKYRAEVTSASQGAITIAPPWSENDRAVGD"
BASE COUNT          743 a         1222 c         1191 g          718 t
ORIGIN      
        1 cgaagtgctt cagtcgacgg cgcgctcgcc gcgagcgttt tccacagcgg cgagatcgat
       61 ttgggcggac tgaagcagta cctggcgtcc aacggcgtag acatccgggt gtgaggacac
      121 tgccatgacg acgcatagcg acagcaaacc tgacagcatt cgctttttgg cgagcctcga
      181 tgagctgatc cgggaacgga tggccgcgag accggcagac agctatgtcg cgtctctcgt
      241 ggcctccggt gacaagcgac tggcacagaa gctcggcgaa gaagccgtcg aactcgccat
      301 cgcagtcacg gccggagacc gcgacgaaca gctcggggaa gccgcggacc tgctatttca
      361 catgctcgtg gcactgaacg cgaaaggcgt tcggctggaa cacgtgagcg cggtactgga
      421 acaacgccat gcgaagatgg ctctgcaaaa gtccgaagaa acgcagtagg atcccgccac
      481 gcgggcgagc gcgatgaacg ttggcgaact cggccgcatt cgccgccccc gaggcaatac
      541 ggcggttcgc gttaattcgc ttccgctata tgtggctcct gcagttgcaa caaatgcctt
      601 agatgagtac ttctttcgcg atcccgcgca tgttgtagtt cgatcccatc acgtagccgt
      661 aagcgccggt gttcagcacc aggatgacgt caccttccct ggatgcgggg agcaggcggt
      721 caatgcccaa gcgatcgccc gtttcacaga tggggccgac gacggtgacg gtatccgttg
      781 ccggttcgtc gagcctcgag aggtttacga tctcatgata ggcgccgtat agcgcggggc
      841 gaatgagcga gttcatgccg gtgccgacgc cgatgtagtg catgtccccc ttgcccttcg
      901 tctgggttac gtgcgtcagg agcacgccgg cgcgcgctac gaggaagcgc cccggctcca
      961 gccacagctc gtaatcgggg caagccttgc ggatctcgat cagcgccgca tcgagctggc
     1021 gcagatcgaa ctgacggtcg cccggccgtt cgggaatccc gaggccgccg ccaagatcaa
     1081 gggcgcgaac atgcggaaaa cgccgcgcca ccttgatcag ttctcccgcg acggtgcgcc
     1141 agttctccgg gtccagaatg ccgctgccgc tgtgcgcgtg aattccgacg actctcgcgc
     1201 cagcttcatc gaccagcctg atcagttcgt ccagctcgaa gcgcggaatg ccgaacttcg
     1261 agtgaacccc tgcagtacgg acgtgggcgt ggtgaccgcg accgtgcccc ggatcgagac
     1321 ggataaagat ctcccggttc ctgaaaattt ccggccactc gcgaagcggg tggaggttgt
     1381 ccagggtcag cagcaagtca ttctctgccg cccactcgta ttcgttgcga ggagcgaagt
     1441 tcagggtgag gagtatgcgc cggccctcga tgcccggtat tacttttcgc aggtgctcca
     1501 cttcaccggg agagacgcaa tcaaaatcca cgccgaggcc tgcgagtgtt tgcaggatat
     1561 gtggattgaa gtttgctttc atcgcgtaca ggacgcggtc aagcgacttc atctgcaaca
     1621 ggtccgttgc ggcagcggtg accgattcca ggtcgtagac gtaggcgttt gcctcggcct
     1681 cggcaatctc gagcaaacgg gatcttttcc tgtgccacca tgcatcctcg cgcggcagcg
     1741 gcgccggacg ctcccggaac agttcctccc aggtcgggcc gaatgcggtg ctgctgctgg
     1801 actggccgat catcgacgag tgcagtttcg agacgagcct gccgacctgg acctggtcga
     1861 tgacaaagct gaaattgaga tcgttcgacg cctgcgacac caggtgaatc ttctcctcct
     1921 cgaacgacga cagtgccggg gcgagccgcg gcaatatcgt gcgaatcttt ctcccgacaa
     1981 ggctgacgac ggcgcagtcg ggaatgaggc gcgcctggca cagcgactcc aggtcgcgca
     2041 tcagcgccat gcgtatttcc tcggagaaca tctcatcggc tgtgtcgatg gacacggtca
     2101 cgttggtttg cgacgtcgaa acgagatcca cggacacacc atgcctgcga aaaactgcga
     2161 aggcttccgc caggaacccg acctcgtgcc acatcgcact gccttccatg gagatcagca
     2221 cggttccgga acgggtcgag atgcccttca cctgcggctc cacttcgagg gtgacggcgg
     2281 atacgacggt gcccgggatg tgcggagccg tcgtgcagcg aatgaaaagc ggaatgccgt
     2341 gcgcccgcag gggcgacagg caacgaggat gcaatacctt gctgcccatc gaagccagtt
     2401 cctgcgcctc gtcgaaatgc agttcgacca gcagccgcgc cgatggcacc aacttcgggt
     2461 cggcggtata catgcccggt acgtctgtcc agatttccag acggcgcgcc tgcagcatcg
     2521 ccgcgaagta cgcggctgag gtgtcagaac cttccctgcc gagcagcacg gttgcgccgc
     2581 gcgagttgct ggcaatgaag ccctgcgtaa gtactacctt gccggaggtg ccgagcgaag
     2641 cctgcaatcc ttcatcggcg gaataactgc aggtcgcgga aagataattc tgcgcctccg
     2701 accggccggc gccgtcctcg gtggagagca tttcgcgggc atcccgcagc tcgcagtcga
     2761 taccgcagcg attgagccac gcagcgccaa gcgttgtcga catcagctcc cccatcgcca
     2821 tcacgcgcgc acgtactcta gagctgactt cctggatcag ccgcacgccg gcaagaagct
     2881 gatccagctc ctgcagggtc ggatcgagca acgcgtgccg gtcgagcccg aggtcgtccg
     2941 caagctggta gtgctgctcc ctgatcgcgg cgatatttgc tgccgggtct ccagaaacgg
     3001 cggcatcgag tgcgtctgca agttcgttgg agataccgcg caatgcggaa tgcacgatca
     3061 gtatcctgag gttttgcgca cggcggttct ggatgagcgc tgcgatcgtg cgccagttgt
     3121 ccgcggtgga cacgctggta ccgccgaact tcatgactac ccagtcggat tttcggatcg
     3181 cctcgctcgc gccgagagcg tcggaaaacg gggcctcttc cacttgcgag tgcagcatcg
     3241 gcggctcagt ccccgacggc acgatcgttc tcgctccatg gcggggcgat cgttatggcg
     3301 ccctgcgatg cggaagtgac ttccgcccgg tacttggcca gcacaccgcc ggttaccgac
     3361 gatggaggcc ttcgccagtg tctgcgccgc tcgacaagca tttcgtcagt tacgtccagg
     3421 tcgacgcgcc tggcagcagc gtcgatcgtg atcgtgtctc cgtcctcgac cagcgcgagc
     3481 ggcccgccga ccgcagcttc cggtgataca tgacctacaa ccaacccatg gctccccccg
     3541 gagaagcggc cgtcggtaat cagtgctacg tcggcgccga gccctttacc tatgatcgct
     3601 ccggtagggc tcagcatctc ccgcatcccc ggcccgccct tcggaccttc atagcggatc
     3661 accacaacgt cgccggcctt gaccacatcg tcgagaatgg cccgcaaggc aagctcttcc
     3721 gaatgaaaaa cgcgcgcgcg acccgagaag cgaagtcctt ccttcccggt gatcttcgcg
     3781 acggcacctt ccggagcgag gttgccgtaa aggatcacga ggtgactgtc ggcctttatc
     3841 ggcctgtcga acgagtgcac gacctcctgg cctt
//
LOCUS       DASUOM010000494         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1388115,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000494 DASUOM010000000
VERSION     DASUOM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388115"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="leuD"
                     /locus_tag="VFY27_10300"
     CDS             <1..562
                     /gene="leuD"
                     /locus_tag="VFY27_10300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5765949.1"
                     /translation="SCTVVMAIDDVDTDQIIPARFLTTTTREGLGRHLFHDWRYDQDG
                     QPTGDFVLNSDASSGCRVLVSGRNFGCGSSREHAPWALLDYGFLAVISSEVADIFRSN
                     ALKNGLLPIIVDDESHRWLLDHPGTVVKVDVAASTLALANGKTVEYPIDAFARYCLLE
                     GLDQLGFLLRHEDAIRSFEESRSWTP"
     gene            562..1611
                     /gene="leuB"
                     /locus_tag="VFY27_10305"
     CDS             562..1611
                     /gene="leuB"
                     /locus_tag="VFY27_10305"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5765950.1"
                     /translation="MASLAGDGIGPEVSEAGQRVLKHIASIRGHHFEVRERLIGGAAI
                     DGVGDPLPPDTIAACLESDAVLLGAVGGPAWSDPNAKKRPEQGLLALRAVLGVFANLR
                     PVQIYNSLSRASPIKPDRLAGVDLVVVRELTGGIYFGEKTRDAQSASDLCRYSTAEIE
                     RIVRVAGSLASQRSGRLCSVDKANVLETSRLWRDVTNRVVADEFPELELEHMLVDAAA
                     MHLINKPRYFDVIVTENMFGDILTDEASMLAGSLGMLPSASLGETRRGLYEPIHGSAP
                     DIAGQGCANPCGMIASIALMFRHSLDLPEEAAMIEQALAKVIEGGARTADIAVAGDTV
                     LSTRDMTAAIIRQLR"
     gene            complement(1592..>2806)
                     /gene="metX"
                     /locus_tag="VFY27_10310"
     CDS             complement(1592..>2806)
                     /gene="metX"
                     /locus_tag="VFY27_10310"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5765951.1"
                     /translation="TSRSPAWRAPTELLPAVCGVLLMVTDASVKGRELLPQYLREHVS
                     AATQYFVLPQPLLLECGRTLERITVAYRTWGNPRNAADNAILICHALTGSADVDAWWP
                     GIISPDGAFDPETDFVVCSNILGSCYGTTGPASLKNGGGERWRADFPRITVRDMVNVQ
                     RALIDHLGIERLALITGPSLGGMQALEWALMYPERVDSIVPIGVGGRHSAWCIAMSEA
                     QRHAIYADPDWNDGNYSEERMPEKGLAAARMMAICTYRSWHSFDGRFCRNEQDSGEFQ
                     VQSYLRYQGEKINRRFDANTYVRLTQAMNDFDISRGRGNYLSVIRSMRQPALVVSVSS
                     DILYPPHEQQLLSQNMPNARHALLQSPDGHDGFLIECRELGSLISKFRERRSAGQAVR
                     PERRSIVSAIAE"
BASE COUNT          523 a          893 c          863 g          527 t
ORIGIN      
        1 atcgtgcacc gtcgtgatgg cgatcgacga cgtcgatact gaccagatca tccccgcgcg
       61 attcttaacg acgacgacac gcgagggcct ggggcgacac ctgtttcacg attggcgcta
      121 cgaccaggac gggcagccga ccggcgactt cgttctgaat tcggatgcca gcagcggctg
      181 ccgggtgctc gtcagcggcc gcaacttcgg ctgcggctcg tcgcgcgagc acgcaccctg
      241 ggccttgctc gattatggtt tcctcgccgt catcagcagc gaggtcgccg acatctttcg
      301 cagcaacgcg ctgaaaaacg ggttgctgcc tatcatagtg gacgacgaaa gccaccggtg
      361 gctcctcgat cacccgggga cagtggtcaa ggttgacgtt gccgcgtcca cgctggccct
      421 cgccaacggc aaaaccgtcg aatacccgat cgatgcattt gcccgatact gcctgctcga
      481 gggtctcgac cagctcggct tcctgctcag gcatgaagac gcgattcgaa gcttcgaaga
      541 gagccgttcc tggacgccgt aatcgcaagc ctcgccgggg acggtatcgg tccggaagtt
      601 tccgaggccg gacagcgggt gttgaagcac atcgcgtcca tacgcggtca tcattttgaa
      661 gtccgcgaac gcctgatcgg cggcgccgcg atcgacggag ttggcgaccc gctgccgccc
      721 gatactatcg ctgcctgcct cgaaagcgac gcggtcctgc tcggtgccgt gggcggaccg
      781 gcatggtcag accccaatgc gaagaagcgc ccggaacagg ggctgctggc gctgcgggcc
      841 gtccttggcg tcttcgcaaa cttgcgaccg gtgcagatct acaactccct gtcgcgcgcc
      901 tccccgataa aaccggaccg gcttgcaggc gtcgatcttg tcgttgtgcg ggagttgacc
      961 gggggaatct acttcggtga gaagacgcgg gatgcacaat ccgcgagcga tctttgccgc
     1021 tacagtaccg ccgagataga acgtatcgtg cgcgttgccg gatcgttggc gtcgcaacga
     1081 agcggcaggc tctgttccgt cgacaaggca aacgtgctgg aaacgtcacg actgtggcgc
     1141 gacgtgacga accgcgtagt ggccgatgag tttcccgagc tggaactcga gcacatgctg
     1201 gtggacgccg ccgcgatgca cctgatcaac aagccccgtt acttcgacgt catcgttacc
     1261 gagaacatgt tcggggacat attgacggac gaagcctcga tgcttgccgg gtccctgggg
     1321 atgctgccgt cggcctcgct gggcgaaacc cgccgcgggc tgtatgaacc cattcatggt
     1381 tccgcgcccg acattgccgg ccagggatgc gccaacccct gcggcatgat tgccagcatc
     1441 gcgctcatgt tccgccacag cctggatctt cccgaggagg ctgcgatgat cgagcaggcg
     1501 ctggcaaagg tcatagaagg tggcgccagg accgccgaca tcgccgttgc cggcgacacg
     1561 gtcctctcga cgcgcgatat gacggctgcg attattcggc aattgcgctg acgattgacc
     1621 ggcgctccgg gcgtaccgct tgtccggcgc ttcgccgttc cctgaacttc gagatgaggc
     1681 ttccgagttc ccggcattcg atgagaaagc cgtcgtgacc gtcgggagac tgcaacaggg
     1741 catgccgcgc gttcggcatg ttctgcgaga gcagctgctg ctcgtggggt ggatacagga
     1801 tgtccgaact gaccgagacc accagcgccg gttgccgcat actgcgaatc accgaaaggt
     1861 aattgccgcg cccgcgcgaa atatcgaagt cgttcatcgc ctgcgtcagg cggacatagg
     1921 tatttgcatc gaagcgcctg ttgatctttt ccccctggta gcgcaggtac gactgcacct
     1981 ggaactcacc gctgtcttgc tcgtttctgc agaatcggcc gtcgaagctg tgccagctgc
     2041 gatacgtgca aatggccatc atccgcgccg ctgccagccc tttttccggc atacgctctt
     2101 ccgaataatt gccgtcattc cagtccggat ccgcataaat cgcatgccgt tgcgcctcgc
     2161 tcatggcgat gcaccaggcg gaatgccggc cgccgacgcc tatcggtacg atgctgtcga
     2221 ccctctccgg gtacatcaat gcccattcga gcgcctgcat cccgccgagc gacggtccgg
     2281 taatgagtgc gagccgctcg atcccgagat ggtcgatcag cgcccgctgg acgttcacca
     2341 tgtcccgcac ggtgatgcgc gggaagtcag cgcgccatct ttccccgccg ccattcttca
     2401 atgaagccgg cccggtggtg ccgtagcagc tacccaggat attgctgcag acaacgaaat
     2461 cggtttcagg gtcgaaagcg ccatccggcg aaatgattcc cggccaccat gcgtccacgt
     2521 ccgccgaacc ggtcagcgca tggcaaatga ggattgcgtt gtcggcggcg ttccgcgggt
     2581 ttccccaggt ccggtaggcg acggtaatcc gctccagggt gcgtccgcac tcgagcaaca
     2641 agggctgggg cagcacgaaa tactgcgtgg ccgccgacac gtgctcccga agatactgcg
     2701 gcagcaattc cctacccttt acggacgcgt ccgtaaccat cagcaggact ccgcaaacgg
     2761 ccggaagaag ctcggtagga gcccgccacg cgggcgagcg cgatgt
//
LOCUS       DASUOM010000495         6411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_787864,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000495 DASUOM010000000
VERSION     DASUOM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787864"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2136
                     /locus_tag="VFY27_10315"
     CDS             <1..2136
                     /locus_tag="VFY27_10315"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5765952.1"
                     /translation="KLAQTIGLLRKRDSRIAALESSLELEAAKLSEAGLAIEQARGEV
                     DSMRAEMEAVKSSQDAAQETIASLNSELAAEKAARKWAEDAEQTQGTRLEQLEAEHHE
                     STSLVATLQQYIDGRKSSWERQEADLKSRARSLKEQRKENARLAREARVTAGRLRNES
                     AARQRLQTERDSLQTGLQQARAEAVTLAESLAAAEKSCRAQQEEVAALAQQVQEGAPA
                     LAHEQGERRRLEAGFAEQQRQRDLLQAELAALQQSYSSKDSLLAEQQDTIEELRQAGE
                     TAARQLAEEQTRSSELEQQLAQLQVETGRLRREQGAFSSEAAERETLLEEQAQEMLRL
                     SAELEAVRISLATQRADHRALLLQHDESTEEITALRSELGPLRGAASKLADLEQANAE
                     LSGLVSSTEDVIRELKGQLAKTEDYADSLRMKLQQQQAETEALCSERDNSAVALREAL
                     ERVDELAGRLEAEQELSARLQLENAEAGMHLEEEVRSVRSELNAAQKTLAENQALSEQ
                     LTADLVESNGKVRMLEKQLAEADKARDSDIRNLSKQVAQFRKQIDDYERKISNKDAAI
                     NALLTELAHKPETPEDNAAADDVVHRLADRRPQPVDDRAILHHERDRVTRLLIGSIDG
                     QELRFPLFKDKLTIGRTVHNDIQLKAQYVSRRHAVIVTEDDHTRIVDWGSKNGIYVNG
                     KRVGEHMLRNGDRVSVGTAEFKYEERPKR"
     gene            2314..2637
                     /locus_tag="VFY27_10320"
     CDS             2314..2637
                     /locus_tag="VFY27_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765953.1"
                     /translation="MATRAAPKQVDPGATQNLTRSLEKLATKKIWLPRLLYDSLPYFY
                     LTAGITAFFAALYISEWFWVVPQYILFSAACLHLAFAVFRRRRKVDAQALDETIVFAP
                     SEGRR"
     gene            2749..3030
                     /locus_tag="VFY27_10325"
     CDS             2749..3030
                     /locus_tag="VFY27_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765954.1"
                     /translation="MKNTLAREAAWFAGLLFGGLVLLPLSIYLVGKAVFGEYGAGSLG
                     DFYADLLGKFIGGEPAVWFLLLSPYLLWQLCRLTIRAFRRSGRPPAPSH"
     gene            3331..3558
                     /locus_tag="VFY27_10330"
     CDS             3331..3558
                     /locus_tag="VFY27_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765955.1"
                     /translation="MVKFRAELNGKIGDGGPGKNVLIRNKFVREESGTHDTLKILDGS
                     LLVEEEEPGGIDPYNTGRFDRAKSWEFRSRK"
     gene            3691..4269
                     /locus_tag="VFY27_10335"
     CDS             3691..4269
                     /locus_tag="VFY27_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX5765956.1"
                     /translation="MGLRVSFDLEEDDLIHFRLIMREARKAAAQSSPEDIIEGATGLL
                     RQVGTVSVPQFIRDRLDKLELMIKMVSDTEWRLPQQESLRVLNALAYFSEPEDLIPDH
                     IPGLGFLDDAIMIELVVRELRHEIEAYSDFCEYRRQRGDRAGTAADQGKAADPTREEW
                     LAARRKELQSRMRRRRKKRSDQANHARPPRLL"
     gene            complement(4306..4584)
                     /locus_tag="VFY27_10340"
     CDS             complement(4306..4584)
                     /locus_tag="VFY27_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765957.1"
                     /translation="MTVITGIVLGSCVSISVSLGAVLFIFLVLGDEYPRLDDEFGALI
                     NSLLIFTAMTAISGMSFYSMLIRHQWRVAAQALMWLGVAATGTYYWPR"
     gene            4772..>6411
                     /locus_tag="VFY27_10345"
     CDS             4772..>6411
                     /locus_tag="VFY27_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5765958.1"
                     /translation="MKNIGIDVRARIVTTLLGLLAFSAFAATPTRVGDAQLAPSSKHE
                     KIGELVTEFVQKSHYEHSSVNDELSAAVLERYIESLDGNRMYLLQSDVDAFQKYRHQL
                     DDMVRSAPMDPVFDMFEVYRTRARERLNFALTQLETEPDFSVDEEFQFDRADAPWAKT
                     ESELNAIWRQRVKNDALGLALTDQEWPEIQETLSKRYSRFLKRMDQIKSDDVFESFMN
                     SFAHTLDPHSSYLSPRSSEEYRIQMSLSYFGIGASLQIEDDYVKVANIIPGGPADIDG
                     ALQPEDRITAVAQGEDGELVDVVGWRLDDVVQLIRGPANSVVQLQILSGNALPGAPQK
                     TISLVRNQVKLEEQAAKSEIVTIPRDGRDWSVGVITVPSFYRDYRALSNGDKDYTSTT
                     KDVKKLIEELEEKGIDGLIVDLRDNGGGHLTEATALSGLFIDNGPVVQLRSSNGRISR
                     LDDPDPVARVAYNGPLAVLVNRYSASASEIFAAAIQDYARGVIIGQQTFGKGTVQNLY
                     SLDQYVRQAEDEEGLGQLTLTIGKYYRVTGESTQHRGV"
BASE COUNT         1455 a         1875 c         1973 g         1108 t
ORIGIN      
        1 aagctggcgc agacgatcgg cctgctccgg aaacgcgaca gcaggatcgc cgccctcgag
       61 tcttctctgg aactcgaggc cgcgaagctc tcggaagcgg gattggcgat cgaacaagct
      121 cgcggcgagg tcgactccat gcgagcggaa atggaggcgg taaagagcag ccaggatgcc
      181 gcgcaggaga cgatcgcgtc cctgaattct gaacttgctg cggagaaggc tgcgcgcaaa
      241 tgggccgagg atgcggagca gacgcagggt acgcgtctgg aacagctgga ggcggagcac
      301 cacgagtcca cgtcgctggt agcaacgctg caacaataca tagatggccg gaagagcagt
      361 tgggagcgac aggaggctga cctgaagtcc agggccagat ctctcaaaga gcagcgaaag
      421 gaaaatgccc gcctcgcgcg ggaagctcgg gtcactgcgg ggcgattgcg caatgagtcg
      481 gccgcacgac agagactgca aacggaacgg gacagcctgc aaacagggct tcagcaggct
      541 agagccgaag cggtcacgct ggcggagtct cttgccgcgg ccgagaaatc gtgccgggcg
      601 cagcaagaag aggtcgcagc acttgcccaa caggttcagg aaggtgcgcc cgccctggct
      661 cacgagcagg gcgaacggcg caggctggag gcaggctttg cggaacagca gcgccagcga
      721 gatttgttgc aggccgagct ggccgcgctg cagcagtcgt actcctcgaa agactccctg
      781 cttgccgagc agcaggacac tatcgaagag ctgaggcagg caggtgagac tgccgccagg
      841 cagcttgccg aagagcagac ccgaagcagc gaactcgaac aacagctcgc gcagttgcag
      901 gtggaaaccg ggcgattgcg ccgggaacag ggtgcattca gctccgaggc agcggaaagg
      961 gaaacgctgc tggaggaaca ggcacaggaa atgttgcgtc tctcggccga actggaggcg
     1021 gtgcggattt ccctggcaac gcaacgggcg gatcaccggg cgctgctgtt gcagcacgac
     1081 gaatcaaccg aggagattac cgcgcttcgc agcgaactcg ggccacttcg tggtgcggcg
     1141 agcaagctcg ctgatctgga gcaggcaaac gcggaacttt ccggcctcgt gtccagcact
     1201 gaggatgtca tccgcgaact gaagggccag ctggccaaaa ccgaagatta tgcggattcg
     1261 ctgcggatga aactgcagca acagcaagct gaaacggagg cactttgcag cgaaagggac
     1321 aactccgccg tcgcgctcag ggaagcgctc gagcgtgtcg atgagcttgc cggacggctc
     1381 gaagcggaac aggagctctc ggcacggctc cagctcgaga acgcggaagc cggcatgcac
     1441 cttgaagagg aagtccggag tgtccgctcc gaactgaacg cggcgcagaa gacgctcgcg
     1501 gagaaccagg cactgagcga gcagctaacc gcagatctcg tcgagagcaa cgggaaggtc
     1561 cgcatgctcg aaaaacagct cgcggaagcg gacaaggcac gcgacagcga catccggaat
     1621 ctgagcaaac aggtcgccca gttccgcaag cagatcgacg attacgaacg caagatcagc
     1681 aacaaggacg cggcaatcaa tgcgcttctg acggagctgg cccacaagcc ggaaacaccg
     1741 gaggacaacg cggcggctga tgacgtggtg caccgcctgg cggaccggcg cccccagcct
     1801 gtggacgatc gcgcaatact gcaccatgag agggaccgag tgacgcgtct cctcatcggc
     1861 agcatcgacg gccaggaact gcgtttcccg ctgttcaagg acaagctgac gatcggacgc
     1921 acggtgcata acgatataca gctcaaggcc caatacgtga gcaggcggca cgctgtcatc
     1981 gtcacggaag atgatcacac gcgcatagtc gactggggaa gcaagaacgg tatctacgta
     2041 aacggcaaac gcgtcggcga gcacatgctg agaaacggcg accgggtgag cgtaggcact
     2101 gctgaattca agtacgaaga acgtccgaag cgctgacatc gttagaccgc tgacggtcca
     2161 cacatttcct cccgcgtgcc gttcaggtca ctttctattt gaaataatgc tgcggcctca
     2221 ccgcgtagcg gtatgtgtga gggtaatcac ggcaattttc acaggagaca gttaccgttg
     2281 ccgcacagca aatcacgata aagaatccgg ccaatggcaa cgagggctgc acccaagcaa
     2341 gttgatccag gtgccacgca gaacctgacc cggagtctcg aaaaactcgc gacgaagaaa
     2401 atctggctgc ccaggctcct gtatgacagc ttgccgtact tttacctgac ggccgggata
     2461 acggcattct ttgccgctct gtatatcagc gagtggttct gggtcgtccc gcagtacatc
     2521 ctgttctcgg ctgcatgcct tcacctcgcg tttgcagtct ttcgcaggcg gcggaaagtc
     2581 gacgcacaag cactcgacga aaccatcgtg ttcgcaccat ccgaaggacg gagatgagcc
     2641 ggcgattcaa cctggggtgt gtccctggtt ggttcgtctt gacctcgacc ggcccatggt
     2701 caaggattgc gctctgctgg tttatttcgg gacacgagtg caggtgatat gaagaatacg
     2761 ctcgcgagag aagcagcgtg gttcgcggga ctgctcttcg gaggactcgt gctcctccct
     2821 ctctccattt acctcgttgg caaggcggtt ttcggcgaat acggtgcagg gagtttagga
     2881 gacttttacg ccgacttgct cggcaagttc atcggcgggg agcccgccgt ctggttcctg
     2941 ttgctctccc cgtaccttct ctggcaactc tgccgcctga caatccgggc gtttcgccgg
     3001 tccggacgcc cgccggctcc gagtcactga caccggcaag tcttgggaat tgtgacaacg
     3061 gtcacatttt ttcgtttgaa tacagtaaat tgccctaaat aagctagaaa ttatgtcaat
     3121 tgtgcgtcat ccttcggcct cgtcaggact atattcgcca ctacaaagaa cgatctcatg
     3181 agcacgtctg ccgcgatgaa atttcaatgc tgtctgaaat ggctgggaac aaagcttttt
     3241 cccagggaag ctgcggtcga gccatcgctg cagcaaacca gcgtcagggt gcggccgcct
     3301 cgcgcacgcc cagccgagac ccgacccgag ttggtaaaat tccgcgccga actcaacggc
     3361 aaaatcggcg acggcggtcc cgggaagaac gttctgatcc gcaacaagtt cgtccgcgag
     3421 gaaagcggga cgcacgacac gctgaaaatc ctggacggtt ccttgctggt tgaagaggaa
     3481 gaacccggcg ggatcgatcc ctacaacacc gggcgcttcg accgggcgaa gtcctgggaa
     3541 ttccgctccc gcaagtaagg cttaacagct tcccggcgcg agctggctgt ttgctccccc
     3601 gcgccgcggc gcccccctgc tctctcaagt ttgacggtct cgcggacgcc gcgtagtctc
     3661 ccatgtccga ttttcgcggg acggcaacat atggggctca gggtttcgtt tgacctcgaa
     3721 gaggatgacc tgattcactt ccggttgatc atgcgggaag cgaggaaagc ggcagcgcaa
     3781 tcttcgcctg aagacattat cgaaggcgcc accggcttgc tccggcaggt cggcactgtc
     3841 agcgtgccgc agttcatccg ggaccgcctc gacaaactcg agctgatgat aaaaatggtt
     3901 tccgataccg aatggcgcct gccacagcag gaatcgctgc gagtgctgaa tgccctcgct
     3961 tatttctccg agcccgaaga cctgatcccg gaccacatcc cggggctcgg tttcctggac
     4021 gatgcgatca tgatcgaact ggtcgtacgg gagctgcggc acgaaataga ggcctactcc
     4081 gacttctgcg agtaccggag acaacgcggc gatcgagcgg gaaccgccgc ggaccagggc
     4141 aaggcggccg accctaccag ggaggaatgg ctggccgccc gccgaaagga actgcagtcg
     4201 cgaatgcgga ggcggcgcaa gaagcgcagc gaccaggcga atcacgcccg gccgccccga
     4261 ttgctgtaga ggtcccggta gcctgcgcgg ccagcgtgtg gtgtcctagc gcggccagta
     4321 gtaggtgccg gtcgcagcca cccccagcca catcagggcc tgtgcggcaa ctctccattg
     4381 gtgccggatc agcatgctgt agaaactcat tcccgaaatc gctgtcatag cagtgaagat
     4441 gagcaaactg ttgatcaggg ccccaaactc gtcatcaagc cgcggatact cgtctccaag
     4501 gacaaggaaa atgaacagga ccgcgcccag gctgacgctg atggaaacgc aggacccgag
     4561 aacgattcct gtaataacgg tcaatggacg catagcttca aatcccggca tgccccaggt
     4621 tcgtggtacc gggacttgcc aataatccgg tttaccaatg ccaatgggcc tcgaaaatgc
     4681 cgatgacgcc cttgattggg acggcgctcg gaactagcct agccactgta agcagatccc
     4741 aaaaggtctc ccgcaccata tggagaaagt gttgaagaat atcggaatcg acgtccgcgc
     4801 aaggattgta accacccttt tgggccttct ggcgttttcc gcgtttgcag cgacgccaac
     4861 tcgcgtcggc gatgcgcagc tggcgccctc gtcgaagcat gaaaaaatcg gggagctggt
     4921 gacggagttc gtgcagaagt ctcattatga gcactcttcc gtaaatgacg aactgtctgc
     4981 cgctgtactc gagcgttaca tcgagtcgct ggatggcaac cggatgtacc tgctgcagag
     5041 cgatgtcgac gccttccaga aataccgaca tcagctcgac gacatggtgc gttccgcgcc
     5101 catggatccc gtgttcgaca tgtttgaggt ctaccggacg cgggcgcggg aacgcctgaa
     5161 tttcgcgctg acccagctgg agaccgagcc ggatttcagc gtcgatgagg aattccagtt
     5221 cgaccgcgcg gatgcgccgt gggcaaagac ggaatccgag ctgaatgcga tatggcgaca
     5281 gcgtgtcaag aacgatgccc tgggcctcgc cctcacggac caggagtggc cggaaataca
     5341 ggaaacgctc agcaagcggt acagccgctt cctgaagcgc atggaccaga tcaagagcga
     5401 cgacgtcttc gagtccttca tgaactcgtt cgcgcatacc ctcgatcctc actcgagcta
     5461 cctgtcgccg cggagctccg aggaataccg gatccagatg agcttgtcgt acttcggcat
     5521 aggcgcctcg ctgcagatcg aggacgacta tgtcaaggtg gcgaacatca tccccggcgg
     5581 tcctgccgac attgacggcg cgctgcagcc cgaagaccgc atcaccgcgg tcgcccaggg
     5641 cgaggacggc gagttggtgg atgtggtcgg gtggcggctg gacgacgtcg ttcaactgat
     5701 tcgcggcccc gccaacagcg tggtgcaatt gcagattctt tccggcaatg ctctaccggg
     5761 cgctccgcaa aaaaccatca gcctcgtcag gaaccaggta aagctggaag aacaggccgc
     5821 caagagcgag atcgtgacga ttccccgcga cggccgtgac tggtcggtcg gcgtcatcac
     5881 cgtcccgagc ttctaccgcg actatcgcgc cctgagtaac ggcgacaagg attacaccag
     5941 cacgaccaag gacgtcaaaa aactgatcga ggaactcgaa gaaaaaggaa tagacggttt
     6001 gatcgtcgat ctgcgcgaca acggcggcgg tcacctgact gaagctacgg cgctctcggg
     6061 actcttcatc gacaacgggc cggtagtgca actgcgcagc tcgaacgggc gcatcagccg
     6121 cctcgatgat cctgatcccg tggcgcgcgt tgcgtacaac ggtccccttg cggtgctggt
     6181 caaccgctac agcgcttcgg cgtcggagat attcgctgcg gcgatccagg actatgcacg
     6241 cggcgtcatc ataggccagc agaccttcgg caagggtaca gtgcagaatc tctactcgct
     6301 cgaccagtac gtgcgccagg cggaggacga ggagggctta ggccaactca cgctgacgat
     6361 cggcaagtac taccgggtga cgggtgaaag cacccagcac cgcggcgtca a
//
LOCUS       DASUOM010000496         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_903676,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000496 DASUOM010000000
VERSION     DASUOM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903676"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VFY27_10350"
     CDS             <1..2035
                     /locus_tag="VFY27_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765959.1"
                     /translation="ALDNMIQGLCMFDEQSNLILCNQRYLDMYSLSADIVRPGCSLRR
                     IIEERKRTNTFEGDTDAYLQEISAIVARGKQAQRFFETPDGRVIRLVHQPMPDGAWVA
                     THEDITIQRRAEEERDRNREFLNLVVDNIPVTTLVKDAKELRYVFVNRAGERYYGTER
                     EKMIGKTPFDLLPGPSAQLIIDLDRQLLAANRGPIDDEHQIRMADGAMRVAKSTRLPI
                     LDEKGEPQYLLTVIEDITERKRAEERIAHLAHHDALTNLPNRVLFRSQLEIAMSQLRE
                     GEHLALLYLDVDHFKSINDTLGHPIGDELLRAIAGRLRRCAQMSDTVARLGGDEFALI
                     HMAIRHPQDCIPLLNCIYEAVRAPYELGGHQVVADVSIGIAIAPGDGTEPDELLKNAD
                     LALYGAKAEGRGTYRFFELDMDARMKERRTLELDLRKAITEGEFELYYQPIRNIAEGH
                     VVGCEALLRWNHPERGIVSPAEFIPVAEETGLIIPIGEWVLRRACADAVTWPDGMKVA
                     VNLSPAQFRSRSLVQTIFNAFATAGLNPDRLELEITETVLMQHTDTTIEMLNQLRKIG
                     VRIAIDDFGTGFSSLGYLRSFPISKLKIDRSFIRDLPDDADALAIVRAVVGLANSLGI
                     VSTAEGVETEEQYEALLELGCSEMQGFLLSRPCPINEVRRFFPAIPHRAVTAA"
     gene            2151..>2355
                     /locus_tag="VFY27_10355"
     CDS             2151..>2355
                     /locus_tag="VFY27_10355"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 family protein"
                     /protein_id="HEX5765960.1"
                     /translation="MSILGKIIDAIFGSSEAKASSTAEGATAGAAAPMQKVDVEAILT
                     KMAAENKEKLDWRRSIVDLMKLLK"
BASE COUNT          507 a          681 c          719 g          448 t
ORIGIN      
        1 ggcgctcgac aatatgatcc agggtctttg catgttcgac gagcaatcga acctgatcct
       61 ctgcaaccag cgctatctcg acatgtatag cttgtcggcc gacatcgtaa ggccgggctg
      121 ttcgcttcgc cggatcattg aggaacgaaa gcgcacgaac acgttcgagg gcgatacaga
      181 tgcttacctg caggaaataa gcgcgatcgt cgctcgcggc aagcaggcgc aacgcttctt
      241 tgaaacaccc gacgggcggg tgatccgtct tgtgcatcaa ccgatgcctg acggggcctg
      301 ggtcgcgacc cacgaagata ttacgatcca gcggcgggcc gaagaggagc gcgatcgcaa
      361 tcgcgaattc ctcaatctgg tcgtggacaa cattcccgtg acgacgctcg taaaggacgc
      421 caaggagctt cgctacgtct tcgtcaatcg cgctggtgag cgctattacg gcaccgagcg
      481 agagaagatg atcggcaaaa cgccgtttga tctgctgccc gggccgagcg cgcaactgat
      541 catcgatctt gatcgccagt tgctggcagc caaccggggc ccgatcgacg acgagcatca
      601 gatccgaatg gctgatggcg ccatgcgggt tgccaagtcg acccgcctcc caattcttga
      661 tgagaaagga gagccacagt atctgcttac ggtgatcgag gatatcacgg agcgcaaacg
      721 cgccgaggaa cgaattgctc acctcgcgca tcacgatgcg ctcacaaatt tgccgaaccg
      781 cgtgctgttc cggtcgcagc tggaaattgc gatgagccaa ttgcgcgagg gcgaacacct
      841 ggcgctgctt tatctcgacg ttgatcattt caaaagcatc aatgacacgc tgggacaccc
      901 gatcggcgac gagttactgc gagcgattgc cggccggctc cgccgctgtg cgcagatgtc
      961 cgataccgtt gcacggttgg gaggcgacga gttcgcgctc atccacatgg caattcgcca
     1021 tccgcaggac tgcattccat tgctcaactg catttacgag gccgttcgcg cgccgtacga
     1081 attgggggga caccaggtcg tggcggacgt cagcatcggc attgccatcg caccgggcga
     1141 cggcacggag ccggacgagt tgctgaagaa tgccgacctc gcactctatg gcgccaaagc
     1201 cgaggggcgc gggacgtacc ggttcttcga gctcgacatg gacgcgcgca tgaaggagcg
     1261 gcggacgctc gaactcgatc ttcgcaaggc aatcaccgag ggtgaattcg aattgtacta
     1321 ccagccgatc cgcaacatcg ccgaagggca cgtagtgggc tgtgaagcgt tgctgcggtg
     1381 gaatcatccg gagcgcggga ttgtttctcc cgcggaattc atcccggtgg cggaagagac
     1441 gggactcatc attcctattg gcgaatgggt gttgcggcgc gcctgcgccg acgcagtcac
     1501 gtggcctgac gggatgaagg tcgccgtcaa tctgtcgccg gcgcagttcc gcagccgcag
     1561 ccttgtccag acgatcttca atgccttcgc gaccgctggc ctcaatccgg accggctgga
     1621 gcttgagatc accgaaacgg tcctgatgca gcacacggac acgacaatcg aaatgctgaa
     1681 tcagttgcgc aagatcgggg tgcgtatcgc gatcgacgat ttcgggaccg gattttcatc
     1741 gctcggctat ctgcgcagtt ttccgattag caagctcaag atcgatcgca gctttatccg
     1801 cgaccttccc gacgacgcgg atgcgctggc gatcgttcgt gcggttgtgg gtcttgccaa
     1861 cagccttggc atcgtttcca ccgcggaggg ggtcgaaacg gaagagcaat acgaagcgct
     1921 cctcgaactc ggctgctccg agatgcaggg atttctgcta agccgtccgt gcccgatcaa
     1981 tgaagttaga cgcttctttc ccgcgatacc ccaccgcgca gtcactgcgg cgtgaacgga
     2041 atgggctcgg tctacataat tccagcgacg agcccgttcg cctgacggaa gttgccgcct
     2101 gccccgaccg gagtaggatt gccacggctt ggggacaacg ggagcggcga atgagcattc
     2161 tcggaaaaat tatcgacgca attttcggaa gcagcgaggc caaggccagc tcgactgccg
     2221 aaggagcgac tgccggcgcg gccgcgccga tgcagaaagt cgacgtggag gcgatcctca
     2281 ccaagatggc tgccgaaaat aaggaaaagc tcgactggcg ccggtcgatt gtcgatctga
     2341 tgaaactgtt gaaac
//
LOCUS       DASUOM010000497         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_176288,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000497 DASUOM010000000
VERSION     DASUOM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176288"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..956
                     /gene="cysW"
                     /locus_tag="VFY27_10360"
     CDS             144..956
                     /gene="cysW"
                     /locus_tag="VFY27_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX5765961.1"
                     /translation="MDRLIPSLLIGLSITFLTLILVVPVASVVGYAFSRGLSVYLAAL
                     QTPDTVAAIRLTLVTAAIVVPLNTIFGVAAAWAIAKFDFRGKQFLISLIDLPFSVSPV
                     VAGLVFVLLFGAQGWFGTWLGERDISIVFAFPGIVLATLLVTFPYVAREVIPVMQLQG
                     REAEEAAVSLGAGGFRTFFRVTLPGIRWALLYGVILCNARAMGEFGAVAVVSGHIRGM
                     TTTMPLHVEILYNEYDLVGAFAVASLLMLLALVTLLVKAVIEALGARRRRSG"
     gene            970..>2430
                     /locus_tag="VFY27_10365"
     CDS             970..>2430
                     /locus_tag="VFY27_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5765962.1"
                     /translation="MRVHAPGLAGWLAIVTALLVTLAVTAVSIAGVRLLRDLAEAEAL
                     TRVELGASTALEALRQSSEDLQNAARVLGERPTLLRMLRGSNLDALDPYLIRYCEGAA
                     LDGCAVVRDGSLLASVGNDLDWQRILDDATEQGQQFLATGAVPGTALVGAQAEVTDLA
                     AVNVLVIRRMDQRFAARLSERVGLEVRVIDFSSFQATGDDFAVINSDALAASAPVADR
                     IRILGVFAASLPVAASTGETIALLQVVLPEAQVMRPVAKLERRMLLIAIIVALLATTC
                     GILIGRHWIDGVKRLTLAARRIGAGDLGASIPAEGGKELGILATTMEEMRRSLVDLTG
                     EIRRREADAQAVLGGIVEGVYAVDQERKIRFLNPQAEKLLKVSATDAIGRFCGDVLKP
                     EADAMGRRPCEHACPILTARRTGSAESREQIQPIPNRVRRVVIASAAPSDGMQVQVLR
                     DETELEAVRRTRDTMLANISHEFRTPLAAQLASIELL"
BASE COUNT          419 a          700 c          765 g          546 t
ORIGIN      
        1 ctcggactgg tgatgctgtg tgtttctttt ctcatgctat tggcaattaa cgcgttgaat
       61 ggctggcgca tgaagcgtct tgtgggaagg cgttgatgat cgggccagcg catgcgatta
      121 cacaaagggc cggctcggat cgcgtggacc gtctgattcc ctcgttgctg atcggcctgt
      181 cgatcacgtt cctgacgctg atcctcgtgg tgcccgtggc ttccgtggtc ggctatgctt
      241 tttcgcgcgg gctctcggta tatctcgcgg cccttcagac acctgacacg gtcgccgcca
      301 ttcgcctgac gctcgtgaca gccgcaatcg tggtcccgct caacacgatt tttggtgtcg
      361 ctgcagcatg ggcaatcgcg aagttcgact tccggggaaa gcagtttctg atctccctga
      421 tcgatctgcc gttctccgtc tctcccgtcg tcgccggcct cgtctttgtg ctccttttcg
      481 gcgcccaggg ctggttcggc acgtggttgg gagagcgcga catctccatc gtgtttgcgt
      541 ttcccggcat cgtgctggcg actctactgg ttacattccc gtacgtggca cgggaagtga
      601 tcccggtcat gcagttgcaa ggccgtgagg cggaagaagc agccgtcagc ctcggcgccg
      661 gaggctttcg aacgtttttc cgggtaacct tgccgggaat tcgctgggct cttctatacg
      721 gtgtcattct gtgcaatgca cgtgcgatgg gcgagttcgg cgcagtcgca gttgtttccg
      781 gacatatccg cggaatgacg accactatgc cactgcatgt cgaaatcctt tataacgagt
      841 atgatctggt cggtgctttc gctgttgcat ccttgctgat gctgctggcc cttgtgacgc
      901 tcctggtgaa ggccgttatc gaggcactcg gggcgcggcg gcgccggtcc ggctgagagt
      961 gtaggaactt tgagagtaca cgcgcccgga ttggctggct ggctcgcgat cgtcacagct
     1021 ttgctcgtga cgcttgctgt tactgccgta tccattgccg gtgtgcgatt gttgcgagat
     1081 ctcgcggaag ccgaagcact gacacgggtg gaactcggcg cttcgaccgc gctcgaagcg
     1141 ctgcgccaaa gcagtgaaga tctgcaaaac gctgcccggg tgcttggcga acgcccgacg
     1201 ctgctgcgga tgctgcgcgg ctcgaatctc gatgctctgg atccctacct gattcgctat
     1261 tgcgaaggtg cagctctcga cggttgcgcg gtcgtgcgcg acggctcgct attggcatcg
     1321 gtcggcaacg atttagattg gcagaggatt ctcgacgacg cgacggagca gggtcagcaa
     1381 ttcctcgcta ccggcgccgt gccgggaacc gcactggtgg gagcacaggc ggaagttacc
     1441 gatctcgcag ctgtcaacgt tctggttata cggcgcatgg accagcgatt cgcggcccga
     1501 ttgagcgagc gagtcgggct cgaggtccgc gtcatcgatt tttcatcgtt ccaggcgacc
     1561 ggcgatgact tcgccgtaat taattcggac gccctggccg cttccgctcc ggtcgcggat
     1621 cgtataagga tccttggcgt gttcgccgcc agcttgccgg ttgccgcgtc aaccggcgaa
     1681 acaatcgcgc tgctccaggt cgtgttacca gaagcccagg tgatgcgtcc tgtggcgaag
     1741 ctggaacgcc ggatgctatt gattgccatc atcgtcgcgc tactcgccac gacttgcgga
     1801 atcctgatcg gacggcactg gatcgacggc gtaaagcggt tgacactggc agcacgccgc
     1861 atcggcgcag gggaccttgg cgcatcgatc cctgccgaag gtggcaagga gctcggcata
     1921 ctggcaacca cgatggagga aatgcggcgc agtctagtgg atcttacagg cgagatccgg
     1981 cgacgtgagg ctgatgcgca agcagttcta ggcggcattg tggaaggagt ttatgccgtc
     2041 gatcaggagc gcaagattcg tttcctgaac ccacaggcgg aaaagctgct taaggtctca
     2101 gcgacggacg caatcggccg cttttgcggt gacgtgttaa aaccggaggc cgatgccatg
     2161 ggcagacgcc cctgtgagca tgcctgtcct atcctgacgg cgcggcgtac cggctcggcc
     2221 gagtccaggg aacagatcca gccgatacct aaccgcgtgc ggcgcgtcgt gattgccagt
     2281 gctgcaccga gcgatggcat gcaggtgcag gtacttcgcg acgaaacaga gcttgaagcc
     2341 gttcgcagga cccgggatac catgctcgcc aatatttccc acgaatttcg cacgccgctc
     2401 gccgctcaac tggcatcgat tgagttactg
//
LOCUS       DASUOM010000498         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_302064,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000498 DASUOM010000000
VERSION     DASUOM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302064"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="mazG"
                     /locus_tag="VFY27_10370"
     CDS             complement(<1..664)
                     /gene="mazG"
                     /locus_tag="VFY27_10370"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX5765963.1"
                     /translation="MEPIEKLREVMARLRDPENGCPWDLEQTFASLAPYTIEEAYEVV
                     DAIRRDDVAGLRDELGDLLLQVVFHSQVAEEAGQFTFDDVAQGIADKMLRRHPHVFGN
                     EEQRKRGAVEGSWERIKAGERAASTPAEDSVLDGIAIALPAMIRAGKLGKRAARVGFD
                     WPDREGPLAKVAEELAELTEAVREGNAAHEAEELGDLLFAVANVARHLDLDPEEALAS
                     ANR"
     gene            complement(680..1312)
                     /locus_tag="VFY27_10375"
     CDS             complement(680..1312)
                     /locus_tag="VFY27_10375"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5765964.1"
                     /translation="MNRKLSARDIRERLADTSQPKDPLRIVMPERLRTWPPEVRENLT
                     TGLKPAGVLIPIIEHEESLTVLLTRRSSALRYHASQVSFPGGRMEAGDADIRATALRE
                     TWEEVGIQPADVDIAGYLEPTPTITGYAVTAVVGIVRSGVELRIDPNEVERAFEVPLA
                     YLLDVANERASEREWNGLRLPIVEFHFASERSWGATANILLELRQRLLKA"
     gene            complement(1321..>2163)
                     /locus_tag="VFY27_10380"
     CDS             complement(1321..>2163)
                     /locus_tag="VFY27_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5765965.1"
                     /translation="THLMDAMPVTMGQELDGWAAQIASSSDRLRDTMKRLTEIAQGGT
                     AVGTGINAHPRFGKKVAVLLTEQTGIEFRQATSLFAALSSPDTAVETSGQLRTLAVAL
                     MKIVNDLRWMNSGPLAGIGEIELPALQPGSSIMPGKVNPVIPEAVAMVCAQVIGNDTT
                     ITIAGQSGNFQLNVMLPLVAHNLLQSIEILTSSVGCLAGTTVADFRVNEENIRRAVGR
                     NPILVTALNPVIGYEKGAAIAKKAYAEGREIREVAREMTDLTESELDRLLDPAALTEG
                     GIKQ"
BASE COUNT          353 a          736 c          632 g          442 t
ORIGIN      
        1 tgcggttcgc ggatgccagc gcttcctcag ggtccaggtc caggtgcctt gctacgttcg
       61 ccacggcaaa caggaggtcg ccgagttcct ctgcctcatg cgctgcgtta ccctcgcgaa
      121 ccgcctccgt cagctcggcc agttcttccg caacctttgc cagcggccct tcccggtccg
      181 gccagtcgaa gcccacccgg gcggcgcgct ttcccagctt cccggcgcgt atcatcgccg
      241 gcagtgcgat cgcgatcccg tcaagcacgc tatcttccgc tggcgtggat gccgctcgct
      301 cgccggcctt gatccgttcc cagctgccct cgacggcacc gcgcttccgt tgttcctcgt
      361 tgccgaacac atgcgggtgg cgccgcagca tcttgtcggc aatgccttgc gccacgtcgt
      421 cgaacgtgaa ctgcccggcc tcctccgcga cctgcgaatg gaacaccacc tgcaacagga
      481 ggtcgcctag ctcatcgcga agacctgcga catcgtcccg ccggatggca tcgacgacct
      541 cgtaggcttc ctcgatcgtg tagggtgcca gcgaagcgaa ggtctgctcc agatcccatg
      601 gacatccgtt ctcgggatcc cgcaggcgcg ccatcacttc acgcagcttc tctataggtt
      661 ccataactta cgtattttat taagctttta agagcctctg acgcaactcg agtaggatat
      721 tcgccgtagc gccccagctc ctctccgagg cgaagtgaaa ctcgacgatc ggcaaacgca
      781 ggccattcca ctcccgctcc gaggcacgct cgtttgccac gtccagcagg taggcgagcg
      841 gcacttcgaa cgcgcgttcc acttcgttgg ggtcgattcg caactcgacc ccggagcgta
      901 cgataccgac gaccgcggtg acggcataac cggtaatggt cggcgtcggc tcgaggtacc
      961 cggcgatgtc tacgtctgcg ggctggatac cgacctcttc ccacgtctcc cgcaatgcgg
     1021 tcgcccttat gtcggcgtcc ccggcttcca tgcgtccgcc ggggaagctg acctggcttg
     1081 cgtggtagcg caatgccgac gagcggcgcg tcagcaacac cgtcagcgac tcttcatgct
     1141 cgatgatcgg gatgagtacg cccgccggtt tcaggccggt ggtcagattc tcgcgcacct
     1201 ccggcggcca cgtgcgcagg cgctccggca tcacgatacg caaaggatcc tttggctggc
     1261 tcgtgtcagc aagccgctcg cgaatatcac gagcggacag tttccggttc acgcctgaca
     1321 ctattgtttg atgccgcctt ccgtcagcgc cgcaggatcg agcaggcgat ccagctcgga
     1381 ctccgtgagg tccgtcatct cgcgtgcaac ctccctgatc tccctgcctt cggcgtaggc
     1441 cttcttggca atggcagcgc ccttctcgta cccgatgacg ggattcagtg cggtgaccag
     1501 gatcgggttc ctgccgaccg cgcggcgaat attctcctcg ttgacccgga agtcggcgac
     1561 cgtggtgccg gcgaggcaac caaccgagct cgtgagtatc tcgatgctct gcagcaggtt
     1621 gtgcgcgacg agtggcagca tgacgttcaa ctggaagtta cccgactggc cggcgatggt
     1681 aatggttgta tcgttcccga tgacctgggc gcataccatc gccaccgctt ccgggatcac
     1741 gggattcacc tttcccggca tgatactgga gccgggttgc agtgcgggca gctcgatttc
     1801 accgattccc gcgagcgggc cggagttcat ccagcgcagg tcgttcacga ttttcatgag
     1861 cgcgaccgcg agcgttcgca gctggcccga cgtttccacc gcggtgtccg ggctgctcaa
     1921 ggccgcgaac agcgacgtcg cctggcgaaa ttcgatgccg gtttgctcag tcagcagaac
     1981 cgccaccttt ttgccgaacc tcggatgagc gttgatgccg gtaccgaccg ccgtcccgcc
     2041 ctgggcgatc tcggtcaggc gcttcatcgt atcgcgcaac ctgtcgctgc tcgaggcgat
     2101 ttgtgcagcc cagccgtcga gctcctgccc catcgtcacc ggcatcgcat ccataagatg
     2161 ggt
//
LOCUS       DASUOM010000499         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1710270,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000499 DASUOM010000000
VERSION     DASUOM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710270"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFY27_10385"
     CDS             complement(<1..697)
                     /locus_tag="VFY27_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5765966.1"
                     /translation="MLTSLLPWAVLLLATLAAGHALLYKRDTRAVIAWVGLIMLVPLA
                     GAILYWMLGVNRVRRRAVALRPQGTLPERVAPEAPAELPDRLAPLARVGDQVGPFLLV
                     PGNSVEPLENGYEAFPAMLDAIASAKLSIALSTYIFDNDAVGAQFADALAEAHSRGVE
                     VRVIIDAVGQHYSWRSMVRRLRSAGIPAEAYMPFLVPGNMSALNLRNHRKLLVIDGKD
                     AFTGGMNIRAGHVL"
     gene            830..1915
                     /locus_tag="VFY27_10390"
     CDS             830..1915
                     /locus_tag="VFY27_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5765967.1"
                     /translation="MSGWLVTLSCRAMLALSAALPATAGDSDAYTVELVALHPGVYLI
                     RRPDPLREPVEANAIFVVNEDGVLVFEGGGAPLVAERTIALIQSVTDKPVTHVVNSHW
                     HGDHNLGNQVYKTMFPDARFIGHPETRGAMTGSPMSYVQRFALSLGDLIADWKAQQRR
                     GELSPRRAAVLPDLDLVREQMLSVTVVAPDLLVSDELVLRPGGREIRVIHLGRGNTPG
                     DLVLWLPGERILASGDLVVHPIPYGFGSFPEEWIKTLDRLATFDFELLVPGHGEPQRD
                     TAYVRRLQAMLTEVRRQVAASVAAGHDLETTRKELDLSMFEREFAGDDEDAKLKFDNW
                     WRQPIARSAWLEARGEEIRQGASDETG"
     gene            complement(1989..>2387)
                     /locus_tag="VFY27_10395"
     CDS             complement(1989..>2387)
                     /locus_tag="VFY27_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5765968.1"
                     /translation="GFDMLYAGPREAVLHAIFRQNMGATHLIVGRDHAGVGSYYGPFD
                     AQAIFEEQVPKGALEIQIFGADNTAYSKKLKRVVMMREAPDHAPEDYVFLSGTKVREM
                     LGQGIAPPPEFSRPEVAKILMDYYQLEMAS"
BASE COUNT          437 a          754 c          769 g          427 t
ORIGIN      
        1 ccagcacgtg cccggcgcgg atgttcatgc cgccggtaaa ggcgtccttg ccgtcgatga
       61 ccagcagttt cctgtggttc ctcaggttca gcgccgacat gtttcccggc accaggaagg
      121 gcatgtacgc ctcggccggt atcccggcag agcgcaggcg ccgcaccatg ctgcgccagc
      181 tgtagtgctg gccgaccgca tcgatgatga cccgtacttc gacgccccgg ctgtgcgctt
      241 cagccagggc atctgcgaat tgagcaccga ccgcatcgtt gtcgaagatg tacgtggaga
      301 gggcgatact gagcttcgct gacgcgatgg cgtcgagcat cgcgggaaac gcttcgtagc
      361 cgttttccag cggctcgacg gaattgcccg gcacgagcag gaagggcccg acctggtctc
      421 caacccgcgc caacggcgcc agcctgtccg gcagctcggc gggcgcctcc ggtgccactc
      481 gttcgggcag cgttccctgc ggtcgcaacg ccacggctcg cctgcgcacc cggtttacgc
      541 caagcatcca gtagaggatc gccccggcga gcggcacgag catgatgagc ccgacccacg
      601 caatcacggc ccgcgtgtcg cgcttgtaaa gcaacgcatg accagcggcg agcgtggcca
      661 acagcagcac ggcccacggc agcaggctag tcagcatatc tctcatcggt ggacagcatc
      721 atagcccgcg ggcgcccgac gcttgtaacc tgatcctatg aactcgcttc cggagacacg
      781 gtcgcatgct gtctgctcca aggtgcgggc gaggataacc ggaggttata tgagcggatg
      841 gctggtgaca ttgtcctgtc gtgcgatgct ggcgctaagt gcagcgcttc ccgcgactgc
      901 gggggatagc gatgcgtaca cggtggagct cgtagcacta cacccgggcg tctacctgat
      961 ccgacggccc gatcctctgc gcgagcctgt agaagcaaac gcgatcttcg tcgtgaacga
     1021 ggacggcgtg ctggtgttcg agggaggagg cgcaccactc gttgccgagc gcaccatcgc
     1081 attgatacag agcgtcacgg acaagccggt tacccacgtc gtcaacagtc actggcacgg
     1141 cgaccataac ctcgggaacc aggtctacaa gacgatgttc cccgatgccc ggtttattgg
     1201 gcacccggaa acgcgagggg ccatgacggg ttcgccgatg tcctacgtgc agcggttcgc
     1261 cctgtccctc ggagacctga tagcagactg gaaagcgcag cagcggcgtg gcgagctatc
     1321 gccacgccgg gctgcggtac tgccggacct ggacctcgtg cgcgaacaga tgctgtcggt
     1381 aaccgtcgtt gccccggatc tcctggtcag cgacgaactg gtgctccgcc ccggcggccg
     1441 cgagatccgg gtaattcacc tgggacgcgg caatacgccg ggcgacctgg tgctctggtt
     1501 gccgggagag cgcatccttg cgagcggcga cctcgtcgtg catccgatac cgtacggttt
     1561 cggcagtttt ccggaggaat ggatcaagac cctcgatcgg ctcgccacgt tcgatttcga
     1621 actgctcgtt cccgggcacg gcgagccgca gagagatacg gcatacgtca ggcgcctcca
     1681 ggcaatgctt acggaggttc gccggcaggt tgcggcgtcc gttgctgcgg gtcacgacct
     1741 cgaaacgacc cgcaaggagc tcgacctgtc aatgttcgaa cgggagttcg ccggcgacga
     1801 tgaggatgcc aaactgaagt tcgacaactg gtggcggcag cctattgccc gttccgcgtg
     1861 gctggaagca agaggagagg agatacggca gggcgcctct gacgaaaccg gctaaccgct
     1921 tccggacctg cgaagaaaaa gaagccccgc ctctcgagcg gggcttctgt tcgatcggct
     1981 gaccggagtc agcttgccat ttccagctgg tagtaatcca tcaggatctt ggccacctcg
     2041 ggtcgcgaga actccggggg tggagcaatg ccctgtccca gcatctcgcg aaccttggta
     2101 ccggacagga agacatagtc ttccggcgcg tgatccgggg cctcgcgcat catgacgacg
     2161 cgtttcagct tcttcgaata ggcggtgttg tcggcgccga agatctggat ttccagtgcg
     2221 cccttcggca cctgttcctc gaatatggcc tgggcatcga acgggccgta gtagctgccg
     2281 acgcccgcgt ggtcgcggcc gacgatcagg tgcgtggcac ccatgttctg cctgaagatc
     2341 gcgtgcagca ccgcctcgcg gggccctgcg taaagcatgt cgaagcc
//
LOCUS       DASUOM010000500         5537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1638435,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000500 DASUOM010000000
VERSION     DASUOM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638435"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFY27_10400"
     CDS             <1..233
                     /locus_tag="VFY27_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX5765969.1"
                     /translation="IIDKFDGGPVGIESLAAALGEERGTIEDVLEPYLIQQGFMMRTA
                     RGRVATRNAYLHFGLAPPAREAMPELPLFPEP"
     gene            244..663
                     /locus_tag="VFY27_10405"
     CDS             244..663
                     /locus_tag="VFY27_10405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX5765970.1"
                     /translation="MENRAEPFAWPVRVYYEDSDAQGLVYFANYFKFMERARTEWLRS
                     LGVEQDELLYEKRRFFVVVDTQADFLIPARFNDELIVTAGLISLARASFLIEQKIHRN
                     TIDGELLCRGTTRAAYLNADTLKPARLPETLFKDSRS"
     gene            660..1349
                     /gene="tolQ"
                     /locus_tag="VFY27_10410"
     CDS             660..1349
                     /gene="tolQ"
                     /locus_tag="VFY27_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5765971.1"
                     /translation="MNTDMSILHLIMDASIPVKLVMLILLAASFVSWSIIFSKRSLIS
                     RTKDASDKFEGNFWSGGDLQTLYRSATREKGGSIGMASIFESGFREFNRGISQENVQP
                     DKLVEECRRAMRVSMMREVDRLEQGLATLATIGSTSPYVGLFGTVWGIMNSFIGLAGQ
                     HTATLAQVAPGISEALIATAMGLFAAIPAVVAYNRYADKVVRLETRYDGFLEEFSSIL
                     QRHSVSKRKGL"
     gene            1352..1795
                     /gene="tolR"
                     /locus_tag="VFY27_10415"
     CDS             1352..1795
                     /gene="tolR"
                     /locus_tag="VFY27_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX5765972.1"
                     /translation="MATPMQKRKLMGEINVVPYIDVMLVLLIIFMVTAPLLTQGVKVE
                     LPKAAAEAITDAPDLKPIVVSVDAAGNLYINLGDDEDKPSSSQDIVARASAVLRRTPD
                     TPILVKADRAVPYGNVVGAMVLLQQAGAENVGFVTDPLDTYPVPE"
     gene            1810..2694
                     /locus_tag="VFY27_10420"
     CDS             1810..2694
                     /locus_tag="VFY27_10420"
                     /inference="COORDINATES: protein motif:HMM:NF018252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5765973.1"
                     /translation="MIRNGYNVVPVLLAVLLHALVLAVMVVGLDLTRRARPVVPLAIQ
                     ATLVSETDMQQAPPVSEPPAAEEPEPEPEPEPPQPDPAEQQRIEAERLERQQELEQEQ
                     ERIRQQQVADQRRLEQEAEERRQREEAERERQQREAAERRQREEAELERQRQEAERQR
                     LEDIERQRLENERRRREAEEAEQARQFQAELAEEQRRLDAMNSGELDRYIFAITRRIQ
                     QAWVPPASAAPGLKCVVQVRQVPGGQVVGVSITSCNGDAAVQRSIEAAVYRASPLPEP
                     DNPALFDRNLNIIFEPEQ"
     gene            2753..4075
                     /gene="tolB"
                     /locus_tag="VFY27_10425"
     CDS             2753..4075
                     /gene="tolB"
                     /locus_tag="VFY27_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX5765974.1"
                     /translation="MTTNFTRRISLFLLAALSLNLASVNALAELEIRITRGAGNQAPI
                     AIVPFGWDGNAPAPIDIAGVVAADLQRSGRFAPVDEADMLQKPTDGVNIDFDDWSILG
                     VEAVVVGKLTQTGENAFSVQFQLFDVFGGEQLVGYRMPATRSTLRRAAHRVADMVYEE
                     LTGIQGVFDTQIAYVTARQGGDQRIYALVVSDADGENEHTIMESPDPIMSPAWSPDSR
                     RLAYVSFEGNASSIFVQTLRTGNRIQVSNRPGINGSPAFSPDGRKLALTLGGTDGNLD
                     IHVLDLASRDVRRLTNNRAIDTEATWSPDGRQVYFTSDRSGGPQVYRVGIDGGTPERV
                     TFEGSYNARPRLSPDGKKLATVHNDRGNFRIAVMTLGQNGVLVLSTGAQDESPSFAPN
                     SDTLIYATRQGGNGVLETVSADGLVRQRLASGEGDVREPVWSPFSLER"
     gene            4167..4700
                     /gene="pal"
                     /locus_tag="VFY27_10430"
     CDS             4167..4700
                     /gene="pal"
                     /locus_tag="VFY27_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX5765975.1"
                     /translation="MTLYKFVGVGLLGFWLASCGSTPVPDPEPIDNDTTDAAARDGSS
                     SGMDSDQLGRGEEFGEDFDDGRGELGMLIYFEFDQADLSPEYADLLARHAARLSNSSR
                     TKVRLEGHADERGSREYNIGLGERRAQAVRRTLLIQGATAEQIATVSFGEERPAAMGS
                     DEESYALNRRVEIKYTN"
     gene            4724..5482
                     /gene="ybgF"
                     /locus_tag="VFY27_10435"
     CDS             4724..5482
                     /gene="ybgF"
                     /locus_tag="VFY27_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX5765976.1"
                     /translation="MKLSAALLPVILLSGCAVTDPADDPVVLKLNDLERRLMSVERIL
                     ASGSLVDIAMQVDNLQRQAAELQGRTETLEYQADEDGERQRDLYEDLDQRIQALEQRL
                     QGVGGSVVDGGTLEPGQLPVPGGSDRDNYQAAFELLKAQRYEPAANAFKQFLASYPDS
                     QLADNAQYWLAESYYVTDQFEEALRHFAVVIDNFPRSRKVPDALLKIGYANYELQRWD
                     EARAALSRVQREHPDTTAARLAEQRLNRMGEAGQ"
BASE COUNT         1139 a         1663 c         1774 g          961 t
ORIGIN      
        1 cgatcatcga caagttcgac ggcgggcccg tcggcatcga gagcctggcc gccgctctcg
       61 gcgaagagcg cggcacgatc gaggatgtac tggagccgta cctgatccag cagggtttca
      121 tgatgcgcac cgcgcgtggt cgtgttgcca cgcgaaatgc ctacctgcat ttcgggctcg
      181 cgccgccggc acgggaagcc atgcccgaac tgccgttgtt ccccgaacct taagtggcac
      241 cttttggaga atagagctga accatttgca tggcccgtac gtgtttatta tgaggactcg
      301 gatgcgcaag gactggtgta cttcgccaac tacttcaagt tcatggagcg agcgcgcacg
      361 gagtggctgc gctcgctcgg tgtggaacag gatgagctgc tgtacgaaaa gcgacgcttc
      421 ttcgtggtcg tcgataccca ggcagacttc ctaattccgg cgcgcttcaa cgatgaactc
      481 attgttacag cgggcctgat cagccttgcg cgagcgagtt tcctgatcga acagaaaatc
      541 catcgcaaca ctatcgacgg cgaattgctt tgccggggca cgacgcgggc cgcatacctg
      601 aacgccgaca ctctgaagcc cgcgcgactg cctgaaactc tattcaagga ctcccgatca
      661 tgaacaccga tatgtcgatc ctgcacctga tcatggatgc cagtatcccg gtcaagctgg
      721 tgatgctgat cctcctggct gcctctttcg tttcctggag catcatcttc tccaagagaa
      781 gcctgatcag ccgcaccaag gatgcgtccg acaagttcga aggcaatttc tggtccggag
      841 gagacctgca gacgctttat cgcagcgcca cccgcgaaaa aggcggcagt atcggcatgg
      901 caagcatctt cgaatccggt ttccgcgaat tcaaccgcgg aatttcgcag gagaacgtgc
      961 aaccggacaa gctcgtcgag gaatgccgcc gcgccatgcg tgtttcgatg atgcgcgagg
     1021 tcgaccggct ggagcagggg ctcgctactc tcgcgaccat cggctccacc agcccttacg
     1081 tcggcctgtt cggtaccgtg tggggcatta tgaactcctt catcggactg gccggccagc
     1141 acacggccac gctcgcccag gtcgcacccg gcatctcgga agctctcatc gcgactgcaa
     1201 tgggcctgtt cgccgctatc ccggcggtcg tcgcgtacaa ccgctacgcg gacaaggtcg
     1261 tgcgcctcga gacccgctac gacggcttcc tcgaagagtt ctccagcatt ctgcaacggc
     1321 attccgtgtc gaagcgcaag gggctgtaga catggcaacg ccgatgcaga agcgcaaact
     1381 gatgggcgag atcaacgtcg tcccgtacat cgacgtcatg ctggtgctgc tgatcatctt
     1441 catggtcacg gcgccgctgc tgacccaagg ggtgaaagta gagttgccta aagccgcagc
     1501 cgaggcgatc acggatgcgc ctgacttgaa gccgatcgtc gtgagcgtcg acgcggccgg
     1561 caacctgtac atcaacctcg gcgatgacga agacaaaccg tcgagcagcc aggacatcgt
     1621 cgcaagagcc tccgcagtgc tgcgcagaac tcccgatacc ccgatcctgg tgaaggccga
     1681 ccgcgcggtg ccctacggca atgtcgtcgg tgccatggtc ttgctgcaac aagccggcgc
     1741 cgagaatgtc ggattcgtga cggacccgct ggatacctac ccggttcctg aatgaccacg
     1801 gcagactgag tgattcgcaa cggttacaac gtcgttccgg tcttgctggc cgtactgctg
     1861 cacgccctgg tcctggcggt gatggtggtc gggctggacc tgacgcggag ggcgcgcccg
     1921 gtggtgccgc ttgccataca ggcaactctc gtcagcgaga cggatatgca gcaggcgccg
     1981 ccggtcagcg agccgccggc ggcagaggag ccggagccgg aaccggaacc tgaacctccg
     2041 caaccggacc ctgccgagca acagcgcatc gaggccgagc gtctcgagcg gcagcaggag
     2101 ctcgaacagg agcaggaacg catccgccag cagcaggttg ccgaccaacg ccggctcgag
     2161 caggaggcgg aagaacggcg ccagcgcgag gaagcagagc gtgagcgaca acaacgcgaa
     2221 gcggcggagc ggcgccaacg ggaggaggct gagctcgagc gacagaggca ggaagccgaa
     2281 cggcagcgcc tggaagacat cgagcggcag cggctggaga acgagcgcag gcggcgcgaa
     2341 gccgaagagg cggaacaggc acgccagttc caggccgaac tcgccgagga gcaacggcga
     2401 ctggacgcga tgaactccgg agaactggac cggtatattt tcgcaatcac ccggcgcata
     2461 cagcaggcgt gggtaccgcc ggcgagcgct gcgccgggac tcaagtgtgt cgtgcaggtg
     2521 cgccaggttc ccggcggaca ggtggtcgga gtgtcgatta cgtcttgcaa cggcgacgcc
     2581 gccgtgcaac gttccatcga ggctgcggtc tacagggcat caccgttgcc ggaacccgac
     2641 aatcctgcgc tgttcgacag aaacttgaac attatcttcg aacctgaaca atagtgccgg
     2701 gaccagctaa tcgatttaca aggtatcgcc ggagatgcca gggataacaa ctatgacaac
     2761 aaactttacg cgccgcattt ccctgttcct gctggcggcc ctttcgctga atctggcaag
     2821 cgtcaatgcg ttggcggagc tggagatccg cattacccgc ggcgctggaa accaggcgcc
     2881 aattgccatt gtcccgttcg gctgggacgg caacgcgcca gcgccgatcg acatagcggg
     2941 agtcgtcgcc gcggacctgc agcgaagcgg aaggtttgcg cctgtcgacg aggccgacat
     3001 gctgcagaaa cccactgacg gcgtgaatat cgacttcgac gactggtcga tactcggcgt
     3061 cgaagccgtt gtagtcggca agctcacgca gacgggagag aacgcgttca gcgtgcagtt
     3121 ccagctgttc gatgtcttcg gcggcgagca gctcgtcggc taccgcatgc cggccacacg
     3181 cagcacgctg cgccgcgccg cgcatcgcgt cgccgatatg gtttacgagg aactcaccgg
     3241 tattcagggc gtcttcgaca cgcagatcgc ctatgtcacc gcaaggcaag gcggcgacca
     3301 acggatttac gcgctggtcg tatccgatgc agatggcgag aacgagcaca ccatcatgga
     3361 atcgccggac ccgatcatgt cgccggcgtg gtcgcccgac agccgtcgtc ttgcttacgt
     3421 atcgttcgag ggcaatgcgt cgtcgatctt cgtgcagacg ctcaggaccg gtaatcgcat
     3481 acaggtttca aaccgtccag gcatcaacgg atcgcccgcg ttctcgcctg acgggcgaaa
     3541 gctcgcactg acgctcggcg gcacggatgg caatctcgat atccatgtgc tcgatctcgc
     3601 aagccgcgat gtgaggcggc tgacgaacaa tcgcgccatt gacacggaag ccacctggtc
     3661 accggacggt cgccaggtct atttcacctc cgaccggagc ggcggcccgc aggtataccg
     3721 cgtcggtatc gacggcggaa cgccggagcg cgttaccttc gaaggcagct ataatgcgcg
     3781 gccaaggctg tcgccggatg gtaaaaagct ggcgacggta cacaacgatc ggggcaactt
     3841 ccgcatcgcg gtgatgacgc tgggccagaa tggcgtactg gtgttgtcga caggcgcgca
     3901 ggacgaatcg ccgagcttcg cgccgaacag cgacacgctg atctacgcca ccaggcaagg
     3961 gggtaacggg gttctcgaga cggtgtcggc ggacggcctg gtacgacagc ggctcgcctc
     4021 gggcgagggt gacgtacgtg agccggtctg gtcaccgttc tcattggaac gctgaagttt
     4081 ccggtttatt taagttcagc aagtaagtat ctgaattacg gggaggcatg cagcccgtat
     4141 tcgcacacat ccacaaggac atcgacatga cactttacaa atttgttggc gtcggcctgc
     4201 tcggcttctg gcttgcgtcc tgcggctcga caccggttcc ggacccggag ccaatcgaca
     4261 acgacaccac tgacgcggca gctcgggacg gttcttccag cggcatggac agcgatcagt
     4321 tagggcgcgg cgaagagttc ggcgaggact tcgacgacgg ccgcggcgag ctgggcatgt
     4381 tgatttactt cgagttcgac caagcggatc tcagcccgga atatgccgac ctgcttgcgc
     4441 ggcacgcggc gcgcctgtcc aacagctcga ggaccaaggt gcggctggaa gggcatgccg
     4501 acgagagggg atcgcgggag tacaatatcg gcttgggcga gcgacgggcg caggccgtgc
     4561 ggcgcacgct gctgatccag ggcgcgacgg cggagcagat cgctacggtc agcttcggcg
     4621 aggagcgccc ggcggcaatg ggcagtgacg aagagtctta tgcgctgaat cgacgcgtgg
     4681 aaatcaaata caccaactga gcgagagtcg cgtgttgaaa gcaatgaaac tgtcggcggc
     4741 actgctgcct gtgatcttac tgtcaggctg tgctgtaacg gatcctgccg acgaccccgt
     4801 tgtgctgaag ctcaatgacc tggagcggcg cctcatgtcc gtcgagcgaa tactggcgag
     4861 cggcagcctg gtcgatatcg ccatgcaggt ggataacctc caacgccagg cggcggagct
     4921 gcagggacgg acggagacac tggagtacca ggcggacgag gacggcgagc ggcaacggga
     4981 tctttacgag gacctcgacc agcggataca ggctctcgag cagagactcc agggcgtcgg
     5041 cggttccgta gtagacggcg gtacgctgga gccggggcag cttccggttc ccggcggatc
     5101 cgacagggac aactaccagg cggcgttcga acttctgaag gcgcagcgct acgagccggc
     5161 cgcgaatgca ttcaagcagt tcctggcgag ctatccggac agccagctcg cggacaatgc
     5221 gcaatactgg ctcgccgagt cctactacgt aacagaccag ttcgaagaag ccctgaggca
     5281 tttcgcggtg gtgatcgata atttcccccg ctctcgcaag gtgccggatg cgctgctcaa
     5341 gatcggttac gcgaattatg aactgcaacg ctgggacgaa gctcgcgccg ctctgtcgag
     5401 ggtccagcgc gagcacccgg acactaccgc tgcgcggctc gccgagcagc gcctgaaccg
     5461 gatgggggaa gcggggcagt aagccgtccg cttgggtttt ttgttgtcgc tccggtatca
     5521 tgccgcgcgg cgacagg
//
LOCUS       DASUOM010000501         8941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1390185,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000501 DASUOM010000000
VERSION     DASUOM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390185"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFY27_10440"
     CDS             <1..308
                     /locus_tag="VFY27_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5765977.1"
                     /translation="VPAVTAFPDYPSIARRDRIEGEAVVCFHIDARGKVVRPSIRSST
                     HRIFEKPAMRAIRRSSFTPLADGEKPAVAKTCRTYRFRLDPINRAALANPSAVRVRA"
     gene            complement(314..1321)
                     /locus_tag="VFY27_10445"
     CDS             complement(314..1321)
                     /locus_tag="VFY27_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX5765978.1"
                     /translation="MKILNLFLSTLAVLVVATDAGHAQSMPTNNRLDIREWLVPWEDS
                     RPRDPYVAGDGRVWFVGQKGDYVANLVPESNQFNRYDLAPDSGPHNLLVDDNNDIWYA
                     ANRARHIGLLDPDTGSITKIEMPDRAARDPHTLTFDSRGDIWFTVQQGNFIGKLTAAD
                     RRVQLIEVPTGRAKPYGIVVDSRDIPWAVAFGTNKLLRVDPQSMTLSEIPLPSKDSRP
                     RRLAVTSDDRVWFADFARGYLGRYNPAADEFAEWPMPGGADSRPYGMAVDRNDKVWIV
                     ETGSTPNRFVGFDAATEEFLSVTEIPSGGGSVRHMDYFAPAGEIWFGTDTNYIGRAKV
                     H"
     gene            complement(1363..2010)
                     /locus_tag="VFY27_10450"
     CDS             complement(1363..2010)
                     /locus_tag="VFY27_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5765979.1"
                     /translation="MAELEIDEGYTKFSVDWSDRSPLLGPAIAELERWRRPLYQAGLV
                     GHYPELNIGYGNISVRAPGSGLFIISGTQTGHLAHTDQRHYALVTRADIAENSIRCTG
                     ATQASSESLTHAAIYELDASVNAVVHVHSESLWEQLKDSVPTTHADVAYGTPEMAGEF
                     KRLYRETRFRDSGVAVMAGHAAGLVSIGGSLEEAARRILAIHAEHKRHSPGGSAS"
     gene            complement(2017..3132)
                     /gene="mtnA"
                     /locus_tag="VFY27_10455"
     CDS             complement(2017..3132)
                     /gene="mtnA"
                     /locus_tag="VFY27_10455"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5765980.1"
                     /translation="MQASGEDFRTIWLKRDDPSVVQIIDQRLLPHEFVVRDLRAWQEA
                     AHAISEMLVRGAPLIGATAAWGLYLAALATPGTADPAESMRRAAEGLGRTRPTAVNLR
                     WAIDRVMACARRDAGSGDLVASLRGEAQAICDEDIEISRGIGEHGLRLIEEIAARKNG
                     GTVNVLTHCNAGSLATINWGTATAPIYFARQRGIDVHVWVDETRPRNQGSQLTAWELA
                     QNGVPHTLVVDNAGGHLMQRGKVDLCITGTDRTTRTGDVANKIGTYLKALAAADNGIP
                     FYVALPSTTIDWTIADGGAIPIEERDPSEVSLIRGKSGGRWLDVQTVTEGTPISNFAF
                     DVTPARLVTGLITERGICDASEEGLRQLFPELAKQAS"
     gene            3291..4445
                     /locus_tag="VFY27_10460"
     CDS             3291..4445
                     /locus_tag="VFY27_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5765981.1"
                     /translation="MGPLSGIKVIEVAGIGPAPFCGMLLSDMGAEVVLIERKAAPLMG
                     FAERSGEAGKRSIVHRGKKSLAMDLKKPAAAAALLRLVADADLLIEGFRPGVMEKLGL
                     GPAECTAVNRRLVYGRMTGWGQHGPLSHCAGHDINYIALAGALYHGGHRESPPAAPPT
                     LVGDVGGGAMLLAVGLLAALLEARQSGEGQVIDAAVSDGAALMTSLLLGLQQQGFWTD
                     QRQANMLDGGSHWYDCYECADGGYVSVGALEPSFYRQLLEKCGLSEDAGLRSQFEPSK
                     WPSQKQRMTELFKSRTRDEWCEILEGTDACFAPVLNMSEAPRHPHNMARRTFVEIDGV
                     TQAAPAPRFSRTPGRIRGAAPASGEHSQAVLHGAGFSEQEIARLRAQDVI"
     gene            4459..6207
                     /locus_tag="VFY27_10465"
     CDS             4459..6207
                     /locus_tag="VFY27_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765982.1"
                     /translation="MRAHLMLPRPVQQNRRLRHRAFRIALPILLLQLAFASQVGAQEQ
                     RAAQEVRTGSGEQTFVADPDMPSGKQLEESGARIGSIILDKQNVFDTSRQDEDKWLYR
                     LANRWHILTRDSVIRQQLLFREGDLFSERLLAESGRILRRNEYLYSAVVEPLSYANGT
                     VDIVVRTRDLWTLMPGLSVSRSGGENKARVTLSESNLLGRGARIRLSYMDDVDRESTN
                     FEFSDEHLGHSWLSFYLGLSDSSDGGRARLLAERPFFALNTSWSAGFEFIDDSREDRL
                     YELGEEIAEYKHESDYYTAFGGWSAGLRNGWVTRWTAGVVYDNQDFSEVPEPEPGLLS
                     VVPEDRRLLYPYIGLEFLEDRYTTTANREQIGRTEDFNLGQQFRATLGFASDRLGSDR
                     NALVYSLEASRGYGDINRKALILSADASGRIDDGSSANTEIGITARWYNQITSKRLFF
                     MTLDARKGHNLDLDNPLEIGGDTGLRGYPLRYQSGDASMLFTIEQRYFTDWYPFRLAR
                     VGGAIFADVGRTWGDNPVQGSPPQGWLRDVGFGLRLGPTRGGAHEVLHIDIAFPLDGD
                     PSIENVQILLESKGSF"
     gene            complement(6284..7195)
                     /gene="folD"
                     /locus_tag="VFY27_10470"
     CDS             complement(6284..7195)
                     /gene="folD"
                     /locus_tag="VFY27_10470"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5765983.1"
                     /translation="MKRATLIDGKAKAAELEKSVTAGAAALLRDSNIKPGLAVILAGA
                     DPASRVYVRNKTRMAESCGFHSIQHTLDAGTPQHEVLALIEQLNEDPSIHGILVQLPL
                     PDHLDAERVTQSVAPEKDVDGFHFVNIGKLSSGSSDDGFVPCTPAGCMLMIEDLHGPD
                     LSGLRAVVVGRSNIVGKPMASLLLRANATVTVCHSRTRDLPEIARTADILIAAAGRPA
                     LVRGDWIKPGATVIDVGINRTEVIENGESKTRLVGDVNFEEACKVAGAITPVPGGVGP
                     MTIIMLMANTLKSAQAAAAVRQGRRYS"
     gene            complement(7221..7706)
                     /locus_tag="VFY27_10475"
     CDS             complement(7221..7706)
                     /locus_tag="VFY27_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX5765984.1"
                     /translation="MKPRLLAFFLLCVSPVPASADDVAVAGMSWMTGCWSYVGAEPGS
                     GEQWMTPAGGSMLGMSRTVKDGETVAFEFLRIAEDDEGVPALFALPSGQAATTFTLLH
                     QTDNEVAFENRGHDFPQRVIYRLTPGPTLIGRIEGTVNGEARSVDFPMKKVDCETADS
                     S"
     gene            complement(7739..>8941)
                     /locus_tag="VFY27_10480"
     CDS             complement(7739..>8941)
                     /locus_tag="VFY27_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5765985.1"
                     /translation="NRHGYMPGKHKGGRAMSVSRLKNISRIGVEQMGNLADSLNDPDL
                     LRLENLDTDMRPPPAAVAFTRRAIDDDDANSYLPFIGLNALREAATRLVSGQSGQEYN
                     WRTECVISAGGLSGILNVLLATLEPGDEVLMTDPTYVGLVNRVYLAGGVPRYVPLVPS
                     AEGWRLDLTALSGIDPAPVRAMLLMSPSMPTGAVFDSDEWQALTGFCRRAGCWLINDS
                     AMERILFDGRTVLHPASFPGMRDRVITVGSASKEYRMIGWRVGWVVGPEKLVADVARV
                     SISNVVCQTGIAMGAVAAAISAEDDGIVDCTRELQARRDLLMTELADFRPILPHGGWS
                     LLIDMSPLDMDGSTASQRLLEKGRVAATSMVNWGSRAAARYLRLVYANESLHRLSGIG
                     QRFRNALL"
BASE COUNT         1603 a         2853 c         2794 g         1691 t
ORIGIN      
        1 gggtgccggc ggttaccgcg ttcccggatt atccttccat cgcccgacgc gacaggatcg
       61 aaggagaagc cgtagtctgc ttccatattg atgcgcgcgg taaggttgtc cgcccatcca
      121 tacgcagcag cacccacagg atcttcgaaa agcctgcgat gagagcgatt cgtcgctcca
      181 gcttcacgcc cctggccgat ggtgagaagc cggctgtcgc aaagacctgc agaacctacc
      241 ggtttcgtct cgaccccatc aaccgggcgg cgctggccaa tccatcggca gtacgcgttc
      301 gagcgtaaat gcgtcaatgc actttggcgc ggccgatgta gttcgtgtcc gtgccgaacc
      361 agatctcgcc ggccggcgcg aagtaatcca tgtgccgcac cgagccgccg ccgctgggaa
      421 tttcggtgac gctcaggaat tcttccgtag ctgcgtcgaa gcctacgaac ctgttcgggg
      481 tgcttcctgt ttcgacgatc cagaccttgt cgttccggtc cacggccatg ccgtaagggc
      541 ggctgtccgc acctcccggc attggccatt ccgcgaactc gtcggcggcc ggattgtagc
      601 gcccgaggta gccgcgggcg aaatcggcga accagacacg atcatccgac gtgacggcga
      661 gccgccgcgg ccggctgtcc ttgctcggaa gggggatctc actgagcgtc atgctctgcg
      721 ggtccaccct cagcaacttg ttcgtcccga aagccacggc ccagggaata tcccgggaat
      781 cgacgacgat tccgtacggt tttgcccgcc ccgttggcac ctcgatcagt tgcacccgcc
      841 ggtctgcggc agtcagcttc ccgataaagt ttccctgctg gaccgtaaac cagatgtccc
      901 cgcgggagtc gaaggtcaga gtgtgcggat cgcgggccgc ccggtccggc atctcgattt
      961 tggtgatgct cccggtgtcc gggtccagca ggccgatgtg ccgggcacgg ttggcggcat
     1021 accagatgtc gttgttgtcg tcgacgagca agttgtgcgg ccccgagtcc ggcgccaggt
     1081 cgtaacggtt gaactggttc gactccggta ccaggttggc gacatagtcg cccttctgtc
     1141 cgacaaacca cacccgtccg tcgccggcaa cgtagggatc cctgggccgg gaatcctccc
     1201 acggcaccag ccattcgcga atatccagcc ggttgttcgt cggcatcgac tgggcgtgac
     1261 cggcatcggt tgcaacaacc agtacggcca gggtgctcaa gaagagattc agaattttca
     1321 attgctctac ctccccgatc gatggtgtcc ttccgcaatc gtctagctcg ccgaacctcc
     1381 gggggaatga cgtttgtgtt cggcatgaat ggcaaggatg cgtcgcgccg cctcctcgag
     1441 gctgccgccg atgctgacga ggccagcggc atgcccggcc atgacggcaa cgcctgaatc
     1501 gcggaagcga gtctcacggt acagtcgttt gaattccccg gccatttccg gcgtcccgta
     1561 ggcgacatcg gcgtgtgttg tcggcaccga atccttcagt tgctcccaaa gactctcgct
     1621 gtgcacatga acgaccgcat tgacggatgc gtccagctcg tagatggcgg cgtgggtaag
     1681 ggattccgac gacgcctgcg tggccccggt gcaacgaatt gaattttccg ctatgtcggc
     1741 cctcgttacc agcgcgtaat gccgctggtc ggtgtgcgcc agatgtcccg tctgggtacc
     1801 gcttatgatg aaaagcccgc tgcccggcgc gcgaacgctg atatttccgt agccgatatt
     1861 gagctccggg tagtgcccga cgagaccggc ctggtagagc ggccggcgcc agcgctcgag
     1921 ttccgcgatg gcggggccga ggagggggga acgatcgctc cagtcgaccg aaaatttcgt
     1981 gtagccctca tcgatctcaa gttccgccat aaatgatcat gaggcctgct ttgccagctc
     2041 ggggaagagc tgcctgagtc cttcttcact ggcgtcgcaa atgccgcgct ccgtgatcag
     2101 tccggtaacc agccgcgcgg gcgtcacgtc gaatgcaaag ttgctgatcg gtgtgccttc
     2161 ggtaaccgtc tgcacatcca gccaccggcc gcccgacttg ccgcggatga gcgagacctc
     2221 cgatggatcc ctttcctcga tgggtatggc cccgccgtcc gcgatcgtcc agtcgatcgt
     2281 cgtcgagggc agggcaacgt agaagggaat accgttgtcg gcagcagcga gagccttcag
     2341 gtaggtcccg atcttgttcg cgacatcgcc tgtacgggtg gtgcgatcgg taccggtaat
     2401 gcaaaggtct actttcccgc gctgcatcag gtgcccgccg gcgttgtcga caacgagcgt
     2461 atgcggcaca ccgttctgcg cgagctccca tgcggtcagt tgcgaaccct ggtttcgcgg
     2521 ccgcgtctcg tcgacccaga cgtgcacgtc gatgcctctc tgcctggcaa agtagatcgg
     2581 cgcggtcgcc gtgccccaat tgatggtcgc gagcgagccg gcgttgcaat gcgtcagcac
     2641 gttgaccgtc ccgccgttct tccttgcagc gatctcctcg atcaggcgga ggccgtgctc
     2701 tccgatcccg cgtgagatct cgatgtcttc atcgcaaata gcctgcgctt ccccgcgcaa
     2761 cgaggctacg aggtcgccgg agcctgcatc ccgcctggca caggccatga ccctgtcgat
     2821 ggcccacctc agattgacgg ctgtggggcg ggttcggcca agaccctccg ccgcccgccg
     2881 catggattct gccgggtcgg cggtgccggg agtcgcgagc gcggcgaggt aaaggcccca
     2941 ggccgcagtt gcgccgatga gcggagcgcc gcgaacgagc atctcgctga tggcgtgcgc
     3001 agcttcctgc cacgccctca ggtcccgaac cacgaattcg tgcggcagga ggcgctgatc
     3061 gatgatctgc accaccgacg gatcatcgcg cttgagccag atcgtcctga aatcttcgcc
     3121 tgatgcctgc aagacctgct tcccggttgc caacccgaaa gttaaacata ccctggcgcg
     3181 ggtgcgccag ccgggcaccg ggggagctgt tgccggcttt ccgggcacag acgacccgtc
     3241 ctgcaagctg ctaccctgtg gatccgcgag gccgcaaatt tgccgaatgc atgggtccac
     3301 tttccggtat caaagtcatc gaagtcgctg ggatcgggcc cgcgccgttc tgcggtatgc
     3361 tgctgtcgga tatgggcgct gaagtcgtcc ttatcgagcg caaggccgcg cctctcatgg
     3421 gcttcgccga gagatcgggc gaggcaggga aacggtcgat cgtgcatcgc ggcaaaaagt
     3481 cgctggcgat ggacctgaaa aagccggccg ccgccgcagc cttgcttcgc ctggtcgccg
     3541 atgccgattt gctgatcgag ggcttcaggc ccggcgtcat ggaaaagctc gggctcggcc
     3601 cggccgaatg cacggctgtg aaccggcgcc tcgtctatgg ccgcatgacc ggctggggcc
     3661 agcatggtcc cctgtcgcac tgtgcgggcc acgacatcaa ttacattgcg ctcgcaggcg
     3721 cgctctacca tggtgggcat cgcgaatcgc cgccggctgc gccgcccacg ctggtcgggg
     3781 acgtcggcgg cggcgccatg ctcctggcgg tgggactgct ggccgcactt ctggaagcga
     3841 ggcaaagcgg cgaggggcag gtcatcgacg ccgcggtcag cgatggcgct gcgctcatga
     3901 cctcgctgct ccttggcttg cagcagcagg gtttctggac cgaccagcgc caggcgaaca
     3961 tgctggacgg tggctcgcac tggtacgact gctacgaatg cgccgacggg ggatacgtgt
     4021 ccgtgggggc gctggagccg tctttttacc gccagctgct cgaaaagtgc ggcctttcgg
     4081 aggacgcagg attgcgctcg cagttcgagc cgtcaaagtg gccgtcgcaa aagcagagaa
     4141 tgactgaact cttcaaaagc aggacgcggg atgaatggtg tgaaatcctg gaaggcaccg
     4201 acgcctgctt tgcgccagtg cttaatatga gcgaggcgcc gcgccatccg cacaacatgg
     4261 cgcgccgtac cttcgtcgag atcgacgggg tcacacaggc cgctcccgcg ccacgattca
     4321 gccggacgcc aggcaggatc cgcggagccg cgccggcaag cggcgagcat agccaggcag
     4381 tgcttcatgg cgcgggcttc tccgaacagg agatcgcccg gttgcgcgcg caggacgtca
     4441 tctgagccag ggacgacaat gcgggcacac ctgatgctac cgcggccggt gcagcaaaac
     4501 cgccgcctgc gccaccgcgc attccgcatc gccctcccga tactgctcct gcagctggcc
     4561 tttgcctcac aggtcggcgc gcaggaacag cgcgccgcgc aggaagttcg cacaggttcc
     4621 ggcgagcaaa cattcgtcgc ggatccggac atgccgagcg gcaaacagct ggaggagtcc
     4681 ggcgcccgta tcggcagcat catcctcgac aagcagaacg ttttcgatac ctccagacag
     4741 gatgaagaca agtggcttta ccggctcgcg aaccgctggc acatcctgac tcgcgactcc
     4801 gtcatccggc aacagctcct gtttcgcgag ggcgacctgt tttcggaaag gctccttgct
     4861 gaatccggcc gcatcctgcg acgcaatgaa tacctctact cagcggtcgt ggaacccttg
     4921 agctatgcca acggcaccgt cgatatcgtt gtgcgcacga gggacctgtg gaccctgatg
     4981 ccgggcctgt ccgtgtcccg gtccgggggc gaaaacaagg cgcgcgtgac tctttcggaa
     5041 tcgaacctgc tcgggcgcgg agcacgcatt cggctctcat acatggacga tgtcgatcgt
     5101 gaatcgacca atttcgaatt ctcggacgag cacctgggcc acagctggct gtcattttac
     5161 ctgggcctct cggacagttc ggacggcggc agggcgcgcc tgctcgcgga gcgaccattc
     5221 tttgccctga acacgagctg gagcgccggc ttcgagttca tcgacgattc cagggaagac
     5281 cggctttacg aactcggcga agagattgcc gaatacaagc acgaatccga ttactacact
     5341 gccttcggcg ggtggtccgc cggcttgcgg aacggctggg tgactcgctg gaccgccgga
     5401 gtggtgtatg acaaccagga tttctcggaa gtgcccgaac cggagccggg cctgttgtcc
     5461 gtcgtgcccg aagaccggcg cctgctgtat ccctatatcg ggctggagtt cctcgaggac
     5521 cggtacacga caaccgccaa ccgcgaacag atcgggcgca cggaagattt caacctcggc
     5581 cagcagttcc gggccacgct gggcttcgcg agcgacaggc tcggttccga ccggaacgcg
     5641 ctcgtatact cactcgaagc gagccgcggc tacggcgaca taaaccgcaa ggcactgatc
     5701 ctttccgcgg acgcgagcgg ccgcatcgac gacgggtctt ccgcaaatac ggaaatcggc
     5761 atcaccgcgc gctggtacaa ccagattacg tcgaagcggc tgtttttcat gaccctggat
     5821 gcccggaagg ggcacaacct ggatcttgac aacccgctcg agatcggcgg agacaccggc
     5881 ctgcggggct atcccttacg ctaccagtcg ggcgacgcca gcatgctctt taccatcgag
     5941 cagcgatact tcaccgactg gtacccgttc cgcctcgcgc gggtcggcgg cgcaatcttc
     6001 gcggacgtcg gcaggacctg gggcgacaat ccggtacagg gtagcccgcc gcagggatgg
     6061 ctcagggatg tcggcttcgg gcttcgtctc ggtcccacgc gcggcggcgc gcacgaagtc
     6121 ctgcatatcg atatcgcgtt tcccctggac ggcgatccgt ccattgagaa cgtgcagatc
     6181 ctgctcgagt ccaagggcag cttctaagca cccacggccg cgcgggtgcg tatacacgaa
     6241 tggtttgggt ggcgcgagac tccgccacct gacggttgcg cgttcatgaa taacgccggc
     6301 cttgcctcac tgctgctgcg gcttgcgccg acttcagggt gttggccatc agcatgatga
     6361 tggtcatcgg gccgacgccg cccggaaccg gagtgatcgc gccggcgacc ttgcaggcct
     6421 cctcgaagtt cacgtccccc accagcctcg ttttcgactc tccgttttca atgacttccg
     6481 tgcgattgat gcctacgtcg atgacggtgg cgcccggctt gatccagtcg ccgcgaacaa
     6541 gcgcggggcg tcccgccgct gcgatcagga tatctgccgt gcgcgcgatt tccggcaggt
     6601 cccgggtgcg gctgtgacag accgtcacgg ttgcgttagc cctgaggagc aggctggcca
     6661 tgggcttgcc gacgatgttg gagcggccga ccacgacggc acgaagaccg gaaaggtctg
     6721 gcccatgcag gtcttcgatc atcagcatgc acccggctgg cgtacagggg acgaagccgt
     6781 catccgaact gccggaggag agtttgccga tattgacgaa gtgaaacccg tcgacatcct
     6841 tttccggcgc gaccgattgc gtaactcttt ccgcgtccag gtgatccggc agcggcagct
     6901 gtacgagaat accgtggatg gacgggtcct cgttgagctg ttcgatcaac gccagaactt
     6961 cgtgctgcgg cgttccggca tcgagcgtgt gctggattga gtggaagccg caggactccg
     7021 ccatccgcgt cttgttccgg acatacaccc ggcttgcagg atccgcgccc gcgaggatga
     7081 ctgcaaggcc tggctttatg ttgctgtccc gcaggagtgc cgcagcgccg gcggtaaccg
     7141 acttttcaag ttcggcggcc ttcgccttgc catcgatgag cgtcgcacgt ttcacaggaa
     7201 cctcgatctg gttccaggtg tcacgagctg tcagccgttt cgcagtcgac tttcttcata
     7261 gggaaatcga cgctcctggc ttccccgttg acagtaccct cgatcctgcc gatcagcgtc
     7321 ggtcccggcg tgagccggta aatgacgcgc tgtggaaaat cgtgcccccg attttcgaac
     7381 gccacttcgt tgtcggtctg gtggagcagg gtaaaggtcg tggcggcctg cccggacggt
     7441 aatgcaaaca gagcgggtac tccttcatcg tcttcagcga ttcgcaggaa ctcgaatgcg
     7501 actgtctcgc catctttgac cgtgcggctc atgccgagca tggatccgcc cgccggcgtc
     7561 atccactgtt caccggagcc cggttctgcg ccaacgtagc tccagcaccc ggtcatccag
     7621 ctcataccgg cgaccgcaac atcgtccgcg gatgccggga ccggcgacac gcacagcagg
     7681 aaaaatgcaa gtaagcgtgg tttcatgcgt gcatctccgg ttatcgggca gtgttcatct
     7741 acaacagcgc gttgcgaaag cgctgaccga tgcctgaaag acgatgcagc gattcgttgg
     7801 cataaaccag gcgcaggtag cgtgcggcag cgcggctccc ccagttaacc atcgacgttg
     7861 cggcaacgcg tcccttttcg agcaggcgtt gcgacgccgt ggagccgtcc atgtcgagcg
     7921 gcgacatgtc gatcaacagc gaccagccgc cgtgagggag tatcggccgg aaatcggcaa
     7981 gctcggtcat cagcaagtcc cgtcgcgcct gtagttcgcg cgtgcagtcg acgatgccgt
     8041 cgtcctctgc ggagatcgcc gcggctaccg cgcccattgc gataccggtc tggcacacca
     8101 cgttactgat gctgacgcga gccacatccg caacgagttt ctccggaccg accacccaac
     8161 cgacacgcca tccaatcatc cgatactcct tcgatgccga acccacggtg atgacccggt
     8221 ctctcatccc ggggaaggac gccggatgca ggacggtacg accatcgaag agtatgcgct
     8281 ccattgcgga atcgttgatc agccagcagc ccgcacgacg gcagaacccg gtcaaggcct
     8341 gccactcgtc gctgtcgaag accgcgccgg tcggcatcga cgggctcatc agcagcatag
     8401 cccggacggg tgccggatcg attccggaca gtgccgtaag gtcgagccgc cagccttctg
     8461 cggagggcac gagaggaacg tagcgcggca ctcccccggc aaggtatacg cgatttacca
     8521 ggccgacgta ggtcgggtcc gtcatgagca cttcgtcgcc aggttccagc gtcgccagca
     8581 ggacattgag gataccggac agccctccgg cggatatgac gcactcggtt ctccagttgt
     8641 actcttgccc cgactggccc gataccaggc gcgtggcggc ttcccggaga gcgttcaggc
     8701 cgatgaaggg caggtagctg ttggcgtcgt cgtcatcgat tgcgcgcctc gtgaaagcga
     8761 ccgcggcagg cgggggccgc atatccgtgt ccaggttttc caggcgaagc aagtccggat
     8821 cgttcaggct gtcggcgagg ttgcccatct gttccacgcc gatcctgctg atgtttttga
     8881 ggcgtgaaac cgacattgcc cgtccccctt tgtgtttgcc cggcatgtac ccgtgacgat
     8941 t
//
LOCUS       DASUOM010000502         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_334145,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000502 DASUOM010000000
VERSION     DASUOM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334145"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="queG"
                     /locus_tag="VFY27_10485"
     CDS             <1..428
                     /gene="queG"
                     /locus_tag="VFY27_10485"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5765986.1"
                     /translation="PYRLDARRCISYLTIELKGSIPLEFRSMIGNRIYGCDDCQLMCP
                     WNKFAVRTDEQDFAPRNNLDSADLTELFCWDEETWEARTEGSAMRRIGFEQWLRNIAV
                     ALGNATGTETVIAALKSREDSASPIVREHVEWALRQHEQ"
     gene            484..1365
                     /locus_tag="VFY27_10490"
     CDS             484..1365
                     /locus_tag="VFY27_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX5765987.1"
                     /translation="MATRPLFTAPHSAFLVPFDGSFRAESSGTTPPDEDRRRPKKRLK
                     AAERQLDDLQRLLLANKTWSVLLVFQGMDAAGIDSTIRAVMSGISPTGCQVFNFKRPT
                     RTELAHDFLWRTTRSLPERGRIGIFNRSQYEEVLVVRVHPEILQNQRLPTAADSAAFW
                     NARLQSIRDQEQHLARNGVAILKFWLNISKNEQKSRFLSRLDEPHKTWKFNPDDVAER
                     QYWDRYMHAYEEALNATSRPWAPWYAIPADSKPYMRAAIAEIIVRSLASLDLQYPEPD
                     ATAMVGFDEARAKLQEE"
     gene            1455..2426
                     /locus_tag="VFY27_10495"
     CDS             1455..2426
                     /locus_tag="VFY27_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5765988.1"
                     /translation="MTGQVLSINSDYKRSLLAGLDLFRGVRPEDVQELLQKCDRRDVQ
                     TGELLLSPGSKNEFVFVVLSGSLNVHVGSPDAPILVTMEAGSCAGEMSIIEDRDPSAY
                     VVAAEPTHLLVIHQSILWDMVDASHEFSKNLLVVLSERVRSHNHFIAASFGNLQKFER
                     NATTDALTGLSNRHTMEETFPREVTRCLQNDQPVSLIMIDVDRFKSFNDKFGHVAGDR
                     ALSAVAQVLQKQFRPRDHLVRFGGDEFAVLLPGIEEAEALVVAERVRDAVSGSTTGSA
                     DSLIQIPVQISMGVAQLTKDGTFDSLLRAADEALYRAKGAGRNTVSR"
BASE COUNT          539 a          747 c          755 g          466 t
ORIGIN      
        1 gtccctacag gctcgatgcg cgccgctgta tttcctatct aaccatcgag ttgaaagggt
       61 ccatcccgct cgagttcagg agcatgatcg gcaaccggat ctacggctgt gacgactgcc
      121 agctcatgtg cccctggaac aagttcgcgg tgcgaactga cgagcaggac ttcgcgccgc
      181 gcaacaatct cgactccgcc gatctcaccg agttgttttg ttgggacgaa gagacatggg
      241 aagcgaggac cgaaggcagc gcgatgcgcc gcatcggatt tgagcaatgg ctgcgaaaca
      301 ttgcagtggc gctgggcaat gctactggaa ccgaaaccgt gattgcggcc ttgaaatcgc
      361 gagaggacag cgcttcgcca attgtgcgag agcatgtaga gtgggcactt cgacaacacg
      421 aacaataatg atggcagcgc tccggcccgc gccacgtacg tataacccgg gaaaagacag
      481 gtaatggcaa cgaggccctt attcacggcg ccgcattcgg cgttcctggt gccttttgac
      541 ggtagctttc gcgccgaaag ttcaggcacc accccgccgg atgaggatcg gcgtcgcccg
      601 aaaaaacggc tgaaagcggc ggagcggcaa ctcgacgacc tgcaacgcct gttgctggcc
      661 aacaagacct ggtcggtgct cctggtattc cagggtatgg acgcggccgg gatagacagc
      721 acgatccgtg cggtgatgag cggtatcagc ccgaccggct gccaggtgtt caatttcaag
      781 cgccccaccc gtaccgaact cgctcatgac ttcctgtggc gaacgacgag gtcgcttccc
      841 gaacgtggtc gtatcgggat cttcaaccgc agccagtatg aagaagtcct ggtggtgcga
      901 gtacatccgg agatcctgca gaaccagcgg ctgccaactg ccgcggattc cgcagcgttc
      961 tggaacgcgc gcctccaatc cattcgggac caggagcagc acctggcgcg caatggcgtc
     1021 gccatcctga agttctggct gaacatctcg aagaacgaac agaagagccg cttcctgagc
     1081 cggcttgacg agcctcacaa gacctggaag ttcaatcccg acgacgtcgc cgaacgacaa
     1141 tactgggacc gttacatgca cgcttacgaa gaagcattga atgcgacgtc gcgaccgtgg
     1201 gcgccgtggt atgcgatacc ggcggacagc aagccttaca tgcgcgctgc catcgccgaa
     1261 attatcgtcc ggtcgctggc gtcgctggac cttcagtacc ccgaacccga cgcgacggcc
     1321 atggtcgggt tcgacgaggc ccgcgcgaaa ctgcaggaag agtgacggga aacagtttca
     1381 tcagaaagtt gtgccgcagt tcgttgtcat tggtccggca ataacgtaaa cagaccctgc
     1441 tgaaaggact actcgtgaca ggccaagtac ttagtatcaa ttcggattac aagcggtctc
     1501 tcctcgccgg gctggacctg ttccgcggcg tgcggccgga ggatgtgcaa gagctgctgc
     1561 agaagtgcga ccgccgggac gtccagaccg gggaactgct gctttctccg ggttcgaaga
     1621 acgagttcgt tttcgtggtg ctttcgggaa gcctcaacgt gcatgtcggt tcgccagatg
     1681 caccgatact cgtcaccatg gaagccggct cctgtgccgg ggagatgtcg attatcgaag
     1741 atcgcgaccc gtcggcttat gtcgtagctg cagagccgac ccacctcctc gtcatccacc
     1801 agtcgatcct gtgggacatg gttgatgcct cgcacgagtt ttcaaagaac ctgctggtcg
     1861 ttttgtcgga acgggttcgc agtcacaacc atttcattgc tgccagcttc ggcaacctgc
     1921 agaagttcga gcgcaatgcg accaccgacg cgcttacggg gctcagcaat cggcacacga
     1981 tggaagagac attccctcga gaagtgaccc gatgcctgca gaacgatcag ccggtatcgc
     2041 tgatcatgat agacgtcgat cgcttcaagt ctttcaacga caagttcggg cacgttgcgg
     2101 gggaccgcgc gctgtcggcc gtcgcgcagg tgctgcaaaa gcagtttcgt ccgcgggatc
     2161 acctggtgcg cttcggcggc gacgagttcg cggttctgct gccaggaatc gaagaggctg
     2221 aagcactggt tgtcgcagaa cgcgtccgcg acgcagtatc gggcagcacc accggcagcg
     2281 ccgattcgct gatccagatc ccggtgcaga tatcgatggg cgtcgcgcaa ctgacgaagg
     2341 acggaacctt cgactccctg ttgcgcgcgg ccgacgaagc gctctaccgc gccaagggcg
     2401 ccggcaggaa caccgtctcc aggtaacgag tcgcccggtc ctcaaacgtc caccacgagg
     2461 ttgtcgatca accgcgcact gccaaggcgt gcagccgcca gaaccac
//
LOCUS       DASUOM010000503         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1148457,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000503 DASUOM010000000
VERSION     DASUOM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148457"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFY27_10500"
     CDS             <1..1071
                     /locus_tag="VFY27_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765989.1"
                     /translation="DSFGRTSVLDLRNDVQVNKVNMYFRGWVFRPEFHYNLWSWTTNT
                     SQGEPAQVVIAGDLGYKFSDKLDLRLGVLPLPGTRTLRGQHPYWNKVDHRTIGNEFFR
                     PSFTTGIIANGQLAKGLYYRAAIANNMSALGVSAAQLDDEFNTVSAGIWWMPTTGEYG
                     TATGYGDFDHHEEVATQFGISATRSRESRQNQPGTEDPENSQLRLSDGTVIFRPDAFG
                     TGGTIEEATYFMTAVDAGVKYRGFALEGEYYFRWIDDFKVAGTIPVTELYDHGFELQA
                     SAMLIPQKLSAYVAGSKIFGEYGDPWDLGLGLNWYPFGERLMRVNTEFLNLQNSPVGY
                     SSVPFAVGGNGWVFQTNLEARF"
     gene            1083..2183
                     /locus_tag="VFY27_10505"
     CDS             1083..2183
                     /locus_tag="VFY27_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5765990.1"
                     /translation="MHSTRNRFARGNAIATRSRCFIFGTLLGGFVVCASTNAAEPVLH
                     DSHFHLTNYVQEGLELSDFLDIMGDRVGRVALFGIPLQQTWAYSNTGDAAPTYYLQSD
                     APLYYYSFVDAAIANAYLSLPETERQRFDPMITGFNPADMYAADHIRRVLETYPGVFT
                     GIGEFTIHKEFVSAKVAGGPPSLTDPALDRIFSFAGEVGLVVLIHNDVDMPFPKAGQD
                     PYSARQLGTMLRRHPNTTVIWAHMGLGRIVHPVPDMAGIMARVLSDPDLAHVSIDLSW
                     SETAKYLVANPEAISVAADLINRYPDRFLFGTDEVAPPDQESYLTTYALYAPLLERLD
                     VTAREKFLKGNYERLFDAARRKVRAWEKENLQ"
BASE COUNT          457 a          729 c          686 g          435 t
ORIGIN      
        1 gattccttcg gcaggacgag cgtgctcgac ctgcgcaacg acgtgcaggt caacaaggtc
       61 aacatgtatt tcagaggctg ggtctttcgt cccgaattcc actacaacct ctggagctgg
      121 accaccaaca cgagccaggg ggagcctgca caggtcgtca tcgccggtga tctcggctac
      181 aagttcagtg acaaactgga cctgcgtctc ggcgtcctgc ccttgccggg aacgcgcacg
      241 ctgcggggcc agcaccctta ctggaacaag gtcgatcacc gcacgatcgg caatgaattc
      301 ttccggccga gcttcacgac cggcatcatc gccaacggcc agctcgcgaa agggttgtac
      361 taccgggccg cgattgccaa caacatgagc gccctgggcg tcagtgcggc gcaactcgac
      421 gacgaattca acaccgtctc ggcagggatc tggtggatgc cgaccacggg ggagtacggc
      481 acggcgaccg gctacggcga tttcgaccat cacgaggaag tcgccacgca attcggtatc
      541 agcgctacgc gcagccgtga gtcccgccaa aatcagcccg ggacggagga ccccgagaac
      601 tcgcaactgc ggctctcgga cggcaccgtc atctttcgac cggatgcgtt cggaaccggc
      661 ggcacgatcg aggaagcaac ctacttcatg acggccgtcg acgcgggcgt caagtaccgc
      721 ggcttcgcgc tggaaggcga gtactacttc cgctggatcg acgatttcaa ggtcgcaggg
      781 acgatcccgg ttaccgagct ttacgaccac gggttcgagc tgcaggcgtc cgccatgctg
      841 atcccgcaga agctcagtgc ctatgtggcc ggttccaaga tcttcggtga gtacggcgat
      901 ccctgggacc tggggctggg tctcaactgg tacccgttcg gcgagcggct gatgcgggtg
      961 aacaccgaat tcctgaacct ccagaactcc ccggtcggct attcgagcgt gccgttcgca
     1021 gtgggcggta acggctgggt tttccagact aacctggaag cgcgattttg agtactacaa
     1081 cgatgcatag caccagaaac cggttcgcgc gcggaaacgc catcgccact cgctcccgct
     1141 gcttcatttt cgggaccttg ctcggcggct ttgtggtctg tgcatcgacg aatgccgcag
     1201 agcctgtgtt gcacgactct cactttcacc tcacgaatta cgtgcaagaa ggtctcgagc
     1261 tatccgactt cctcgacatc atgggagaca gggtagggcg ggtagccttg ttcggcatcc
     1321 cgttgcagca aacctgggct tattccaaca ccggcgatgc cgcgccgacc tattacctgc
     1381 agagcgacgc gccgctctac tactattctt tcgtcgacgc ggcgattgcg aatgcatacc
     1441 tgtcgctccc ggaaaccgag cgacagcgtt tcgatccgat gatcacggga ttcaacccgg
     1501 ccgacatgta tgcagcagac cacatccgcc gcgtgctgga gacgtacccg ggtgtgttca
     1561 ccggcatcgg cgagttcacc atccacaagg agttcgtgtc ggcgaaggtc gccggcggac
     1621 ccccgagcct cacggatccc gcgctcgacc gaattttttc ctttgctggt gaagtgggtc
     1681 tcgtcgtgct gatccacaac gatgtcgaca tgccgttccc caaggcaggc caggaccctt
     1741 actcggcccg gcagctcggc acgatgttgc ggcggcaccc gaacacgacg gtgatctggg
     1801 cgcacatggg actcggtcgc atcgtgcacc cggtcccgga catggccggc atcatggcac
     1861 gggtgctgag cgaccccgat cttgcacacg tcagcatcga tctctcatgg agcgaaaccg
     1921 ccaagtacct ggtcgcgaac cccgaggcga tctccgtcgc ggctgacctc atcaaccgtt
     1981 acccggaccg cttcctgttc ggcaccgacg aggtcgcgcc accggaccag gagagctacc
     2041 tcacgacgta cgcactctat gcgccgctgc tcgagcgcct tgatgtgaca gctcgcgaga
     2101 agttcctgaa gggtaactac gaaaggttgt tcgatgcggc ccgccgcaag gtgcgggcgt
     2161 gggaaaagga gaacttgcag tgaactctcg tcaacctgct gaaccattcc gtcgagaaca
     2221 tcatgtgggg cggtgagctt caggggggcg agcgcgagaa caactccgac ggcttcacgt
     2281 cggacgacct gcgtcttcaa ttctctt
//
LOCUS       DASUOM010000504         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1227067,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000504 DASUOM010000000
VERSION     DASUOM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227067"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..216)
                     /locus_tag="VFY27_10510"
     tRNA            complement(142..216)
                     /locus_tag="VFY27_10510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Thr,seq:ggt)
     gene            complement(252..325)
                     /locus_tag="VFY27_10515"
     tRNA            complement(252..325)
                     /locus_tag="VFY27_10515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Gly,seq:tcc)
     gene            complement(610..694)
                     /locus_tag="VFY27_10520"
     tRNA            complement(610..694)
                     /locus_tag="VFY27_10520"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(658..660),aa:Tyr,seq:gta)
     gene            complement(830..1342)
                     /locus_tag="VFY27_10525"
     CDS             complement(830..1342)
                     /locus_tag="VFY27_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5765991.1"
                     /translation="MLTESYQKFHRDLGIPSDYASSRKLPHYAEARDLVDVGPNLVGR
                     MQRLTPEAAGRWQRMASAAADDGIRLLIVSGFRSFDYQAQLIRKKLEAGQAIGEILRV
                     NAAPGFSQHHTGTAVDVASPGSRPLTEDFENSEAFRWLGENAGRFGFSMTYPRANRWG
                     FIYEPWHWSL"
     gene            1564..2304
                     /gene="cmoA"
                     /locus_tag="VFY27_10530"
     CDS             1564..2304
                     /gene="cmoA"
                     /locus_tag="VFY27_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HEX5765992.1"
                     /translation="MMSKDEIYASDDADSRPFEFNENVARVFPDMLRRSIPGYDASIE
                     AIGWLASRYVRTNTRCYDLGCSLGAAAEAIRDNVAETGVQIVAVDSAPAMVERCRQRL
                     AARGREDDPRPKVEVVAADVREVAIDNASMVVMNYTLQFLPPGAREPMMRTIARGLNE
                     GGVFFLSEKVVHEDEVIETLLVDLHHQYKRRNAYSELEISRKRAALENVLIPERIETH
                     RRRLAGAGFRHVGVLLQYLNFISLVATR"
     gene            2307..3287
                     /gene="cmoB"
                     /locus_tag="VFY27_10535"
     CDS             2307..3287
                     /gene="cmoB"
                     /locus_tag="VFY27_10535"
                     /inference="COORDINATES: protein motif:HMM:NF011650.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HEX5765993.1"
                     /translation="MLALASLVEELAAHGLPEWREELRPLLEAKLADGAHGDLPKWRE
                     ALAALPRCRAEPAELDRPVIAVCPTGLRPADREVVRELLQKLSPWRKGPFQIGDILID
                     AEWRSDLKWARIENRISPLAGRLVLDVGCGNGYYALRMRGMGARAVVGIEPMLLYVAQ
                     HVAIRHFMQPEPVYVMPLRLQELPPAEVFDTAFSMGVLYHQREPRQHLEALLRMLKDD
                     GELVLETLILPGELREARTPQRYARMKNVWLLPTRPLLLEWLQEAGFDEARIESVSAT
                     TNLEQRRTEWMRFDSLAEALDPQDPSTTVEGWPAPQRALVIARKPARKPP"
BASE COUNT          666 a         1082 c         1042 g          674 t
ORIGIN      
        1 ttcgttctct caaattttcc tttcaacata actccgttcc tgggtggtca gggaacacat
       61 ttcacatctg ttcccggttt gctgtccggt cgccaaggga cagatttaaa tctgtcccct
      121 tttttctgaa tctgtccctt ctggagccca cacccggatt tgaaccgggg acctcttcct
      181 taccaaggaa gtgctctacc cctgagctat gtgggcaata gctatcttga gtctcgcact
      241 gccaccaaga ctggagcggg tgatggggat cgaacccaca tcatcagctt ggaaggctga
      301 ggttctacca ttgaactaca cccgcccggt attcattagc cacgtcagcg ccgcgcagga
      361 aactccgaca gttccgacat cagactgtcc ggattttcaa catcgcttgc gtgtcttcct
      421 cagagagccg ccgacaccgt cgcccgatca atgaacgtgt gtactgcggt tattgctgct
      481 cgcataaaag tgccgggtga aatagttgct atacttgcga cgtacacaac caccaccggc
      541 acgctcaagg ccctgacctg gttttctggc tgtcgcgtcg cctccacctt ggaccgggat
      601 tccgggacct ggtggagggg gtaggattcg aacctacgta ggctaagcca gcagatttac
      661 agtctgccct cgttgaccgc ttgagtaccc ctccgcggac acaagccggc tattgtctaa
      721 ccctcccacc tgactgtcaa cacgggaaat catctaaatg gccggcgcca ggcggctctt
      781 aacacggact ttaccctcag gatcccggtc gaattaccgc gaccgccgct cacagtgacc
      841 agtgccaagg ttcatagatg aacccccagc gattggcgcg tgggtaggtc atcgaaaagc
      901 cgaatcggcc ggcgttttcg ccgagccagc gaaaggcttc tgaattttcg aaatcctccg
      961 tcagtggtct gctgccgggg ctggcaacgt ctactgctgt gccggtgtgg tgttggctga
     1021 acccgggcgc cgcattgacc cgcagaattt ctccgatggc ctggcccgcc tccagcttct
     1081 tgcggatgag ctgcgcctgg taatcgaagc tcctgaagcc cgacacgatc agcagccgga
     1141 tgccgtcatc agccgcggca gatgccatcc gctgccagcg accggcagct tccggcgtca
     1201 gccgctgcat gcgtcccacc aggttcgggc cgacgtccac gaggtcccgc gcctccgcat
     1261 agtgtggcag cttccgcgag ctcgcgtaat cggacggaat tccgaggtcg cgatgaaact
     1321 tctggtaact ctccgtgagc atgtgcagcc cctgacgatc gccctcgttg attcacgaac
     1381 gggcggcgaa gattgtcaca cacacatgag gaacggcaag ccggggggac agatttactg
     1441 aatctgtccc ataaccctgc agaccaccgt aagcgacgca gatataaata aatctgtccc
     1501 ctcttgcacc tttgcgcgtt ttgcaccttg tagaatggcc cgctacacca ggcatgagca
     1561 tcgatgatgt ccaaggatga gatctatgcg tccgacgacg cggattcccg cccgttcgag
     1621 ttcaacgaaa acgtcgcaag ggtcttcccc gacatgctgc ggcgttccat cccgggctac
     1681 gacgcatcga tagaggcgat aggctggctg gcgtcgcgct acgtcaggac gaacacccgc
     1741 tgttatgacc tcggctgctc gctgggcgcg gccgccgagg cgattcgtga caacgtggca
     1801 gagacaggcg tgcagatcgt ggcggtagac agcgcgccgg caatggtgga gcgctgccgg
     1861 caacgactgg cggcccgggg ccgcgaggac gacccccgcc cgaaagtcga agtcgtggcg
     1921 gcggacgtga gggaggttgc catcgataat gcttccatgg tggtcatgaa ctacaccctg
     1981 caattcctgc cgcccggggc acgcgaaccg atgatgcgga ccatcgcccg gggcctgaac
     2041 gagggcggcg tcttcttcct gtcggagaag gtggtgcacg aggacgaggt gatcgagacg
     2101 ctgctcgtgg acctgcacca ccagtacaag cgcaggaatg cctatagcga actcgaaatc
     2161 agtcgcaagc gcgcggcgct cgagaacgtc ctgatcccgg agcgcatcga aacccaccgc
     2221 cgtcgtctcg ccggggccgg ttttcggcac gtgggcgtgc tattgcagta cctcaatttc
     2281 atatccctgg tcgccaccag gtaaggatgc tggcgctcgc gtccctggtc gaggaactcg
     2341 ccgcgcacgg tcttccggag tggcgcgaag aattgcggcc gctcctcgag gcgaagctgg
     2401 ccgacggtgc gcacggagac ctgccgaaat ggcgcgaagc gctggcggcg cttccccggt
     2461 gcagggccga acccgccgag ctcgacaggc cggtaattgc cgtctgcccc accggtctcc
     2521 ggcctgccga tcgcgaggtc gtccgggagt tgctgcagaa gctgtcacca tggcgcaaag
     2581 ggcctttcca gatcggagac atcctgatcg acgcggagtg gcgctccgac ctcaagtggg
     2641 cacgcatcga aaacaggatc tcgccgcttg ccggccggct ggtactggac gtcggctgcg
     2701 ggaatggtta ctacgcgctg cgcatgcgcg gcatgggcgc gcgggctgtt gtcggcattg
     2761 agccgatgct gctgtatgtc gcgcagcatg tcgcaattcg ccacttcatg cagcccgagc
     2821 cggtttacgt catgcctctt cgcctgcagg aactgccgcc ggcggaggtt ttcgacactg
     2881 cgttttccat gggtgtcctc tatcaccagc gggagccgcg gcaacacctc gaggcgctgc
     2941 tccggatgct gaaggacgac ggggaactcg tgctggagac gcttatactg ccgggagagc
     3001 tgcgcgaagc caggacgccg cagcgctacg cacggatgaa aaatgtctgg ctgcttccaa
     3061 cccggccgct gctcctcgaa tggctgcagg aagcggggtt tgatgaggcg agaatcgaga
     3121 gcgtctcggc gaccacgaac ctcgagcagc gccgcaccga atggatgcgt ttcgattcgc
     3181 tggcggaggc gctcgacccg caggacccga gcacgactgt cgagggctgg ccggcgccgc
     3241 agcgcgcact ggtcatcgcc cggaaacctg ctcgcaagcc accttgacga gcggctgttc
     3301 ctgatcttta atttctgtcc taacagaaat ggtcgaaact catgaacctg tcgccggcgg
     3361 tagaaaagta cgtcctgcac tggggcgaga tgggcacccg ctggggcacc aaccgcaccg
     3421 tggcgcagat ccaggcgctt ctttacttgt cccccaagcc gctc
//
LOCUS       DASUOM010000505         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1148537,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000505 DASUOM010000000
VERSION     DASUOM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148537"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFY27_10540"
     CDS             complement(<1..343)
                     /locus_tag="VFY27_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5765994.1"
                     /translation="MSARLTDAKLAGDRRGFKKAMRRLSGTAYKQCDPLGDDQEVVTL
                     EVDAKQQPVESEYDRKFAEWCLSLAGNPRISIKLWDGTEYYSGKQAPIAGMEIRDRAS
                     LLALLRSLSMGF"
     gene            complement(495..914)
                     /gene="mscL"
                     /locus_tag="VFY27_10545"
     CDS             complement(495..914)
                     /gene="mscL"
                     /locus_tag="VFY27_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5765995.1"
                     /translation="MLKEFKEFAMRGNVVDMAVGIIIGAAFGKIVSSLVADVVMPPIG
                     LLMGNMDFSQLYLNLSGTEYPSLAAAQEAGAATINYGLFINAVLDFLIVAFAIFMVIR
                     GMNKLKRKAEEAPAEPPKASTEVQLLTEIRDSLRARS"
     gene            complement(1057..2970)
                     /locus_tag="VFY27_10550"
     CDS             complement(1057..2970)
                     /locus_tag="VFY27_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5765996.1"
                     /translation="MFPANPATNRILVVDDDKEIVSEYLHCLGSNFEPDLATTTLSDL
                     EKVLFGEQTDEKGSARFEVDSRDQGVAGVAAVEHAVQANKPYAIVFLDIRMPPGINGV
                     EAATRIRRVDPNVNIVLVSGSLLPEPEKLGTQIPPADRIFFFKKPFHAIECRQLAAAL
                     CGKWHADLALRRANEVLEQRVIDRTADLHRLAYFDPVTKRPNRLKLTDELQVMIGAGT
                     DGRTALVLLDIERFSFLNETMGYEAGTAVLRAIASRLERALECRLGARRSVVGRFGAD
                     EFACLMEDVRDETELQELVDYIRQAVMEPFVVNGRDLFLKASMGVAWHPEHGNDPRTI
                     FRCAESALHRSMRRPDGYVTFYHSEMQFRARHQFNMEAELRQAIDNGEITAFFQPQVE
                     LKDGRLAGVEALARWIRPNGTIVRPADFIPLSEEMGISDVLFECIMRRVCKTVRSWRD
                     SYGWDVPVSVNLSAHQLRNQQLVQLVGSILEQFSPGKKLINLELTETALLEDLTVARP
                     VLTDLAALGVGIHIDDFGTGYSSLSYLAELPAQTLKIDQTFVQRISESPNNERVVQAI
                     VALGKAMNLEVIAEGVETPQQLELVTRFGCDLAQGFLIAKPMPEKDFLAWWEAHGACG
                     KTSLRVSSAVATR"
     gene            complement(2981..3472)
                     /locus_tag="VFY27_10555"
     CDS             complement(2981..3472)
                     /locus_tag="VFY27_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5765997.1"
                     /translation="MNTQPDNSIRVLIADDDEHILECYRSAFGSSNATSFVDTLSCLE
                     AELFDQQPANEAAPRFDVVACSQGRDAISLAVEARQSGRPFDVVILDVRMPPGIDGVE
                     AGSRIRQFDPEVEIVFVTGFSDLTMSELASRVPPPVKLHYFNKPLSFMQLAESVADMV
                     RTG"
     gene            complement(3453..>5447)
                     /locus_tag="VFY27_10560"
     CDS             complement(3453..>5447)
                     /locus_tag="VFY27_10560"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX5765998.1"
                     /translation="VLPMRGRLASLSLQHKVSVTLLVVMASLVLLSYLVLTTTVAPAF
                     ANLEQATAEKNIVRTERAIQNDLQNLSATVGDWAPWDDAHAFARGENPGFPRSNLDLP
                     TMVNLNVDLLVIYNARGDVLWCHLLQDGKSVDYDSLGILGSDSPESARLIHHRELGSR
                     VEGLVQTDLGPMQLSSQPIVMSDKSGPIAGTMLMGRFFDEATREALAQRTEVALNFHL
                     LDEKPLGDADLQAKILAAGPQAFTYAATESDIQAYSVLTDLFGKPLMILQADTPRQIS
                     ALGNKTVNGALLFLLAAGVVVAIVTWFLLRRIIVLPLERLASHISGIRETGDLSLRLN
                     ETRSDEIGELAVEFDKMTQEVNEARRQLLDQSFKAGKADTAAEVLHNIRNAMTPLING
                     IDRLSKNFRFTGGLRIREAADELGDPGCQPERRSKLLQYIVSAFGHIQKSSEDAISDL
                     QVASRQARQVEAILSDQERYANVAPVIESLDLTSVIEEASLVIPDQSKPGVRLVVPPG
                     LDTLRVRAHRVGLVQVLGNLILNACESIQRGQTAAGSISMAAVHEVLDGQPMVRLTIT
                     DSGCGFTEDVKHSIFQRGFSSKEGHQRGLGLHWCANALSNMGGRISAESDGKDRGASF
                     HVLLPAATGQKSTARELQSAPSRTANSDDSQRLTGEYATG"
BASE COUNT         1033 a         1654 c         1609 g         1151 t
ORIGIN      
        1 cgaagcccat tgatagcgaa cgaagcaggg ccaggagtga tgccctgtca cggatttcca
       61 tcccggcaat cggcgcttgc ttgccggaat agtactcggt gccatcccac agcttgatgg
      121 aaatgcgcgg gttgcctgcc agggacaggc accattcggc aaattttcga tcgtactcgc
      181 tctcgaccgg ttgttgtttg gcatcgactt ccaaagtaac cacctcttga tcgtcgccta
      241 agggatcaca ttgtttgtac gccgtgccac tgagccggcg catcgctttt ttgaaccccc
      301 tcctgtcgcc cgccagtttc gcatcagtga gccgcgcgga caagcgcccc ggacggtgta
      361 cccgaagcac gccttcagta ttccaggctt ctggtgttca agcgttattc gattctcctc
      421 atgccgcggc gggagagtgg aggcatcgca aattccgtgg ccggtgcggg aagcagcaac
      481 ggaaaacgtc gacgtcaact tctggcgcgc agggaatccc ttatttctgt cagaagctga
      541 acctcggttg acgccttcgg cggctctgcc ggtgcttctt ctgcctttct cttgagcttg
      601 ttcatgccgc ggatgaccat gaaaattgcg aaggcgacga tgagaaaatc gagtacggca
      661 ttgatgaaga gtccgtagtt gatggtcgca gcacctgcct cctgcgcggc agcgagggac
      721 gggtattcgg tgccggacag gttcaggtac agctggctga aatccatgtt tcccatgagc
      781 aacccgatgg gaggcatgac gacgtctgcc accagtgaac tcacgatctt gccgaatgcc
      841 gcgccgatga tgatgccgac cgccatgtcc accacgtttc cgcgcatcgc aaattctttg
      901 aattctttaa gcatgacttt tccccttagc cagtgattat ttgatgccac ctcggactgc
      961 agcgacctgc gtagcagcgg cgtgatcaat tctaggcagg accctgctct cgccgcaaca
     1021 aaacggcaga ctacgagggc ctggaaagcc aggaaactag cgggttgcta cggcagagga
     1081 aactcgcaga ctggttttgc cacaggcgcc gtgggcttcc caccacgcaa ggaaatcctt
     1141 ttccggcatc ggcttcgcga tgagaaaccc ttgcgcaaga tcgcacccga aacgcgtcac
     1201 gagctcgagc tgttgtggag tctcgacacc ctcggctatc acctcgaggt tcattgcctt
     1261 gcccagtgcc acgatggcct ggacgacgcg ttcattgttt ggcgactccg aaattcgctg
     1321 gacaaacgtt tggtcgatct tcagggtctg cgcaggcagc tctgccaggt agctgagcga
     1381 ggaataaccg gtgccgaaat cgtcgatgtg gattccgacg ccaagcgccg cgagatcagt
     1441 gagcaccggc cgggcaaccg tgagatcctc gagcagtgcc gtctccgtca attcgaggtt
     1501 gatcagcttc ttccctggtg agaactgttc caggatggag ccgacgagct gcacgagctg
     1561 ctggtttcgg agctggtgcg cggacaggtt gacgctgacc gggacatccc agccgtagct
     1621 gtcgcgccag ctgcgaactg tcttgcagac cctccgcatg atgcattcga acaggacgtc
     1681 cgatatgccc atctcctcgc tcagcgggat gaaatcagcc ggtctgacga tcgtaccgtt
     1741 cggcctgatc catcgagcaa gtgcctcgac acctgccaat cgtccgtctt tcagctctac
     1801 ctgcggctgg aagaaggccg tgatctcacc gttatcgatc gcctgccgca gttcggcttc
     1861 catgttgaac tggtgccgcg cccggaattg catctcgctg tggtagaacg ttacatatcc
     1921 atccggccgc cgcatggacc tgtgcagcgc ggactccgca cagcgaaaaa tggtcctggg
     1981 gtcgttgccg tgctccggat gccaggcgac tcccatgctg gctttcagga acaggtcgcg
     2041 tccgttgacg acgaacggct ccatcacggc ctgcctgatg tagtccacca gctcctgcaa
     2101 ttcagtttcg tccctgacat cctccatgag gcatgcgaat tcatccgcac cgaacctgcc
     2161 aaccacactg cgccgcgccc ccagccggca ttctagcgcc cgctccaggc ggcttgcgat
     2221 ggcgcgcagc acggcggtac ccgcttcgta tcccatggtc tcgttcagga aactgaagcg
     2281 ctcgatatca agcagcacga gcgcagtgcg tccatccgta ccggcgccta tcatcacctg
     2341 cagttcgtcg gtcaatttca gtcgattggg acgcttcgtc acaggatcga agtaagcaag
     2401 ccgatggagg tcggccgtcc ggtctatcac ccgctgctcc agtacttcgt ttgcgcggcg
     2461 caaggcgagg tccgcgtgcc acttgccgca aagcgcagcg gccagctggc ggcattcaat
     2521 cgcatggaac ggctttttga agaaaaaaat gcggtcggcg ggaggaatct gcgtgccgag
     2581 tttttccggt tccggaagca gcgagccgct cacgagcacg atgtttacgt ttggatcgac
     2641 cctgcgaata cgggtcgcag cttcgacgcc gttgatcccg ggaggcatac ggatatccag
     2701 gaagacgatg gcgtatggct tgttcgcctg caccgcatgc tcgacagcgg cgacccccgc
     2761 caccccctgg tcccgagagt cgacctcgaa cctcgccgac cctttctcgt cagtctgttc
     2821 tccgaaaaga accttttcga gatcgcttaa ggtggtcgtc gcaagatccg gctcgaaatt
     2881 gctgcccagg cagtgcaggt actcacttac aatttccttg tcgtcatcga cgaccaggat
     2941 gcgattggtg gcgggattgg ctggaaacat gccgtctcgc tcagccggtc cgaaccatgt
     3001 cagcgacgct ttcggccagc tgcatgaagg acaggggctt gttgaagtag tgcagtttca
     3061 ccggcggtgg tacacggctg gcgagttcgg acatcgtaag gtcggagaat ccggtgacga
     3121 acacgatttc cacttccggg tcgaactggc gtatccggct gcctgcttcg acaccatcga
     3181 ttcccggtgg catgcgcacg tccaggatga cgacgtcgaa gggcctgccg ctttgtcgcg
     3241 cctcgacggc gagcgaaatc gcatccctgc cctggctgca ggcgacgacg tcgaacctgg
     3301 gcgcggcttc gttcgctggc tgttggtcga acaattccgc ttccaggcag ctgagagtgt
     3361 ctacaaagct ggttgcgttg ctactgccga acgccgaacg gtagcactcc aggatgtgct
     3421 cgtcgtcgtc agcgataaga acacgaatgc tgttatccgg ttgcgtattc acctgtcaac
     3481 ctctgtgagt cgtcgctgtt ggctgtacgg gatggcgcag actgcaactc gcgggccgtg
     3541 ctcttctgcc cggtcgcggc cggcagcagc acgtggaacg acgcgccacg atccttaccg
     3601 tcactttcgg ccgaaatgcg gccacccatg ttcgaaagcg cgtttgcgca ccagtgcagg
     3661 ccgagtccgc gctggtgtcc ttccttggac gagaaacctc tttggaaaat gctgtgcttg
     3721 acgtcttcgg taaacccgca accggaatcc gtgatggtca gccgcaccat cggctggccg
     3781 tcgagtacct catggaccgc cgccatcgat atgcttcccg ccgcggtttg ccctcgctgg
     3841 atagattcac aggcattgag gatcaggttt cccagcacct ggacgagccc gacgcgatga
     3901 gcccgaaccc gaagcgtgtc cagcccggga gggaccacga ggcggacgcc gggtttgctc
     3961 tggtcgggaa tgacgagcga cgcttcctcg attaccgagg tcaggtccag gctctcgatc
     4021 accggagcga cgttggcgta ccgttcctgg tcgctcagga tcgcctccac ttgcctggcc
     4081 tgccgcgacg ccacctgcag gtcgctgatc gcatcctcgc tcgacttctg gatgtggccg
     4141 aatgcggaca caatgtattg cagcagcttg ctgcggcgct ccggctggca gccgggatcg
     4201 ccaagctcgt cagccgcttc gcgaatgcgc agcccgcccg taaacctgaa gttcttcgac
     4261 agccggtcta tgccgtttat caaaggcgtc atcgcattgc ggatgttgtg cagcacttcg
     4321 gcggcggtat ccgccttgcc tgctttgaat gactggtcga ggagctggcg cctggcttca
     4381 ttgacttcct gggtcatctt gtcgaattcc accgccagtt caccgatctc atcgctgcgc
     4441 gtttcattca gccgcagcga aagatccccg gtttcacgta ttcccgatat gtgactggcc
     4501 agccgctcga gcggcaggac gataatccga cggagcagga accacgtgac gattgcaacg
     4561 acgactcccg cggcaaggag aaacagcaat gcgccgttaa cggtcttgtt acccagcgcc
     4621 gagatctgac ggggcgtgtc cgcctggaga atcatcaggg gcttgccgaa caggtccgtc
     4681 agtacgctgt acgcctggat gtcgctttcg gtagcggcat aggtgaaggc ctgcgggccg
     4741 gcagccagga tttttgcctg gaggtctgca tcgccaagcg gcttctcgtc cagcaggtga
     4801 aagttgaggg caacttccgt tcgttgcgcg agcgcttccc gtgtcgcttc gtcgaagaac
     4861 cgccccatga gcatggtgcc cgcgatcggc ccgcttttgt cgctcatgac gatcggctgc
     4921 gaactcagct gcattggtcc aaggtctgtt tgcaccaggc cctctacacg gctcccgagt
     4981 tcccggtgat ggatgagcct ggcggattcc gggctgtcgc tcccgagaat gcccagcgag
     5041 tcgtaatcca cgctctttcc gtcttgcaac aggtggcacc acaggacgtc gcccctggcg
     5101 ttgtagatga ccagcaggtc tacattgagg ttgaccatcg tgggcagatc gaggttggag
     5161 cggggaaagc cgggattctc tccgcgggcg aaagcgtgcg cgtcatccca gggcgcccag
     5221 tcgccgacgg tcgcagaaag gttctgcagg tcgttctgga tggcacgttc cgtacgcacg
     5281 atgttcttct cggcagtggc ctgctccagg ttcgcgaacg cgggagcgac ggtcgtggtg
     5341 agtacgagat aactcagcag caccagggac gccattacca caagaagcgt cacagacact
     5401 ttgtgctgca gggaaagtga tgcaagtctt ccgcgcatgg gcaggac
//
LOCUS       DASUOM010000506         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_721452,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000506 DASUOM010000000
VERSION     DASUOM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721452"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..851)
                     /locus_tag="VFY27_10565"
     CDS             complement(135..851)
                     /locus_tag="VFY27_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5765999.1"
                     /translation="MTITEALCLFAAGAANILGALAVVSHKHWSKSAIEMLIALAAGF
                     MLAVSLLDIIPESMSVHGETALLVVLAGYFLVHLTQHTLTEHFHFGEETHAVSRLVGQ
                     TALVGLLLHTFVDGVAIVSAFESDEQLGLLVFMAITLHKFPEGLAVASICIAAGMGRK
                     LALMAAMILGLATLLGGILTGPIAVLRDFGLPLSGGVTLYVAASNLVPEFQAKRGWRL
                     PLFFLLGCVLYFVSRSLLLR"
     gene            complement(851..>2131)
                     /locus_tag="VFY27_10570"
     CDS             complement(851..>2131)
                     /locus_tag="VFY27_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX5766000.1"
                     /translation="GGYEAWCVGGAVRDALLGQHHLDWDIATSATPDQVRRTFRRTIP
                     IGVEFGTVGVLDRTNVMHEVTTFRRDVETDGRHAVVKFGVSLEDDLARRDFTINAIAW
                     SPTRGQLFDPFDGAGDLERGLLRTVGEAGMRMAEDRLRALRAFRFAARFGFSIDPETW
                     SAVAASAKYLSRLSAERVKQEIEKTMDQVSRPGTAFRMWMRSGALRELVPAISRASDL
                     ELGFADFLAQPRLPNRPARRINRITALLLPAGKHAHDTLRALRFSNSDVAWVSGQVER
                     WQSTLPDLEASLGRTGTVPPAALRRWISSVGRMRAPALLRVAVAVWCARQDAGDEVIA
                     LRHARALYGRVVRSSFRDPLELGDLKVDGDDLREAGVPPGPRLGKLLNTLLGHVLEDP
                     SLNDRDKLMALSRQLSSETNDGGRRKSGSGDGKR"
BASE COUNT          483 a          661 c          617 g          370 t
ORIGIN      
        1 gcaagtcgtc ctgaccaacg aactcgtcga gggttgatgg gcgcatccgc gccgcgagcg
       61 gacgcgccgg tgcgcttgac tcaaacagtg aagctgcagc tcgatcgccg gagcgcggaa
      121 tcatgatcca gccattaccg aagaagcagc gaccgtgaaa cgaagtagag aacgcagccg
      181 agcaggaaaa aaagaggaag gcgccagccg cgcttggcct ggaactcagg taccagattt
      241 gatgccgcca cgtagagcgt tacaccgccc gacaacggca ggccaaaatc ccgcagtact
      301 gctatgggac cggtaagaat tccgcccagc aacgttgcaa gtccaaggat catagccgcc
      361 atgagcgcaa gttttcttcc catccccgca gcaatgcaga tgctggcgac cgccagcccc
      421 tccggaaact tgtgaagcgt aatggccatg aatacaagca atcccagctg ctcgtcgctc
      481 tcgaatgcgc ttacaatcgc aactccatca acaaaggtgt gtagcaggag tccaaccagt
      541 gctgtttgtc ccaccaaccg actgaccgcg tgcgtttcct cgccgaagtg aaagtgctcg
      601 gtcagcgtgt gttgtgtgag atgcaccagg aaatacccgg caagaacgac aagcaatgcc
      661 gtttctccat gcacgctcat cgactcgggg ataatgtcga gcaacgaaac tgcgagcatg
      721 aagccggcgg caagtgcgat gagcatctca atggcgctct tgctccaatg tttgtgcgaa
      781 acgacagcga gcgcgccaag gatatttgcc gctccggcag caaaaaggca caaggcctcg
      841 gtaattgtca tcagcgtttt ccgtcgccgg agccggactt cctgcgaccc ccgtcgttcg
      901 tttcagacga gagctgacgc gaaagggcca tgagcttgtc tctgtcgttc agcgagggat
      961 cctcgagtac gtggccgagg agggtattca ggagcttccc caaacgagga cccggaggaa
     1021 ctccagcctc gcgaagatcg tctccgtcca ccttgaggtc gccgagttcc agcggatcgc
     1081 ggaaagaact tcggacgacc cggccgtaaa gagcgcgggc atggcgaagc gctataacct
     1141 catcacccgc atcctggcga gcgcaccaca ctgcgacagc gacgcgcaaa agggctggtg
     1201 ctctcatgcg ccccacgctc gaaatccagc ggcgcagagc agccggcgga actgtgcctg
     1261 tcctgccgag cgaagcttcg agatcaggca gcgtcgactg ccagcgttca acctggcccg
     1321 aaacccaggc aacgtcggaa ttggagaaac ggagagcacg tagagtgtca tgcgcgtgct
     1381 ttccggccgg caggagcagt gcggtgattc gattgatgcg acgggcggga cgattgggca
     1441 gccgcggctg cgcgagaaaa tccgcgaagc caagctccag gtcggacgct cgggaaatag
     1501 ccggaaccaa ttcacgcaat gcaccgctcc gcatccacat cctgaacgca gtgcccggac
     1561 ggctcacctg atccatcgtt ttttcgattt cctgcttgac acgttccgcc gacagtcgcg
     1621 agagatattt tgcgctggca gctacggcac tccacgtttc cggatcaata ctgaaaccga
     1681 aacgcgccgc gaatcggaag gctcgaagcg cacggagccg gtcttcggcc atccgcatgc
     1741 ccgcttcacc taccgtacga agcagacccc gttccagatc accggcgccg tcgaagggat
     1801 cgaatagctg gccccgggtt ggcgaccacg ctatcgcatt aatggtgaag tcacggcgtg
     1861 ccagatcgtc ctcgagcgag acgccgaatt tcactacggc gtgccggcca tcggtctcga
     1921 catctctgcg gaacgtggtc acctcatgca tcacgttcgt ccggtccagc acaccgaccg
     1981 tcccaaactc tacaccgatc ggtatcgttc tgcggaaggt gcgacgaacc tggtccggcg
     2041 tggccgatgt ggcgatgtcc cagtccagat gatgttgtcc aagtagagca tcccgcacgg
     2101 cgccgccgac acaccaggct tcgtatccgc c
//
LOCUS       DASUOM010000507         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_665627,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000507 DASUOM010000000
VERSION     DASUOM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665627"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFY27_10575"
     CDS             <1..792
                     /locus_tag="VFY27_10575"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5766001.1"
                     /translation="HLNSIAVRDIPRIAELLHGAQGRGLRITTEAYPYGAGSTVVGAE
                     LFRGNWRERLGGASAADIELAGVPFNDATLAEAQEKTPGEWIVAHYLRPDRSVVDQKH
                     LDQSVLFPGGAIASDGMRWTLNGEVVTGDVWPLPPEAFAHPRSAGTFTRFLRDYVLER
                     KRETLREGLRRMSLIPAQILETSVPQMTKKGRVQVGADADLVVFDLATVSDRGTYTHP
                     NQTAVGMRYVIVNGVPVIRDGTLVRDALPGRSIRRPVASHQAIAR"
     gene            complement(1013..1459)
                     /locus_tag="VFY27_10580"
     CDS             complement(1013..1459)
                     /locus_tag="VFY27_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766002.1"
                     /translation="MTSVKDKLELIGIVAVMVSLLILVYEIRQNTDTSAAQAVFELNE
                     SARQAQFQEATDPNLASLIIKAEKDFEALSERERYMYSRWVFAHVNLFESAWRYNRRG
                     VVSDAEIRGWKTAFCGYMARASFRQVVESLEIFTPEFAEESAQWCE"
     gene            complement(1575..2012)
                     /locus_tag="VFY27_10585"
     CDS             complement(1575..2012)
                     /locus_tag="VFY27_10585"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5766003.1"
                     /translation="MSWALAASPATTALADEATSAFINVQPDEVSYEPDPEAPELGYA
                     VLSGDPAKVGVYVIRLRIPPGLAFPPHYHDQDRHITVISGVWAFGKGRSGSCDDTVPM
                     TAGAYVMHPKGGIHYDGACGDEPVEVQIIGEGPVKTTWIDEPE"
     gene            complement(2117..2869)
                     /locus_tag="VFY27_10590"
     CDS             complement(2117..2869)
                     /locus_tag="VFY27_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766004.1"
                     /translation="MSTRFNWRFLLGEFVVIVAGVLMALWVDQLREARVNAQLEVEYL
                     ESLVIDLDSDLAQFDETEAWMRRQEAAAATVLALYEGSPPTDNVADLVAAVETAGWQY
                     APSITRNTLEDLRSTGNLRLIRDPELRRAIAAYYTTVEEISIPLADIRDRIWAQYDAR
                     VANVLGPSVRLGVLQGPESFGHGITSDAIEAAEAPDLEELMAALRAFPELEIAAGEVL
                     YQTINSRAGLDGMRRAALELKTRLEQWLDSRE"
     gene            complement(2939..>4128)
                     /locus_tag="VFY27_10595"
     CDS             complement(2939..>4128)
                     /locus_tag="VFY27_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5766005.1"
                     /translation="RALKLGEPTADTLAAAGLVEELRVITASDTAAKERALRYYENAV
                     AKDPNNILALQWLASIYLDINQADKSREMFERVVELDPLNTLALTGLSNALFGLGRYD
                     EGRVHLYRMRALFPELGMIYRYLSGMEDELGRADKSTYWMERAIELDPNPFEITFALI
                     SYTAFGWADEALDAAARYNQSSGGVDISRLVQAQLDRQFDAIAAEARILFERNGATRF
                     AVLAAWADAIAGRCDAAVPSLERQFPSLQGETLQYLDATDLIDAVLLAHCYAENGGQR
                     ESGRLTRLLLTSEMLSDDAVRYRPRLRLVRVAAHAVAGDIDTALTELQLIEAGKAPLA
                     ISAIALPVDELPVFEALYGEEPFRQYARQERYQIAQQARMLASGQTEQEIKAQIESAG
                     FVF"
BASE COUNT          809 a         1290 c         1205 g          824 t
ORIGIN      
        1 cacctcaaca gcatcgccgt gcgtgatatc ccacgcattg ccgagctgtt gcatggcgca
       61 caggggcgcg gcctcaggat cacgaccgag gcatatccgt acggcgcagg gtcgaccgtc
      121 gtcggcgcgg aactgttccg cggcaactgg cgcgaacgcc tgggtggcgc aagcgcggct
      181 gacatcgagc tcgcgggcgt cccattcaat gacgcaacgc tcgccgaagc gcaggaaaag
      241 acgccgggag agtggatcgt tgctcactat ctgcgtcccg accgcagcgt agtggatcag
      301 aaacatctgg accaatcagt tctctttccc ggcggtgcga tcgcatctga tggcatgcga
      361 tggacgttga acggcgaagt cgttacgggc gacgtctggc cgctgccccc ggaggccttc
      421 gcgcatccac gcagcgccgg aacgttcacc cggttcctgc gggactacgt cctggagcgc
      481 aagcgggaaa cgctgcgcga aggattgcgg cggatgtcgc tcatcccggc gcagattctc
      541 gagacgagcg tgccacagat gaccaagaag gggcgtgtgc aggtgggtgc cgacgcggac
      601 ctggtggtgt tcgatctggc caccgtttcg gatcgcggga cctatacgca cccaaaccag
      661 accgccgtag ggatgcgcta cgtcatcgtc aacggcgtgc ccgtgatccg cgacggaacg
      721 cttgttcgag acgcgctgcc gggccggtca atccggcgtc cggttgcttc gcaccaggcc
      781 atcgcccgct aaaccgcgtt ggcggatact gactgacggc gcggccagag acgccaggaa
      841 agccgtcgcc gacatcatcg tggaaaggca cagacgttga ttctcgtcga taccggcccg
      901 ctggttgcct tgttcgaccc cgcggacgac tcccatgcgc attgcgttcg tgtgttgcag
      961 tcggtcgaag agccaccgcc acgacgatcc ccgttctcag atgtttcaca cttcattcac
     1021 accattgagc ggattcttcg gcaaactccg gcgtaaatat ttcgagagac tcgactacct
     1081 gccgaaaaga cgctcgcgcc atatacccgc agaaggctgt cttccagcct cttatctcag
     1141 catcactgac aacgccgcgg cgattgtacc tccatgcaga ctcaaacaaa ttcacatgtg
     1201 cgaagaccca gcggctgtac atgtagcgtt cgcgttcact cagagcctca aaatccttct
     1261 cagccttgat gattaacgaa gccaaattcg gatcagttgc ctcctgaaat tgcgcctgtc
     1321 gggcagattc attgagctca aataccgctt gagcggcgga ggtgtcagtg ttctggcgaa
     1381 tttcataaac gagaatcaag agcgatacca taaccgcgac gatgccaatt agctccaact
     1441 tgtctttgac tgaagtcatc cggctcccgg tccaatctat ggtgcgttag tgtctcggct
     1501 ttgggttgaa cccgcagtca aaagcagcca ggtaacaacc tcaaatgcca ccgtcacatg
     1561 tcggctggaa tcaactattc aggctcatca atccaagtgg tctttaccgg cccttcccca
     1621 ataatctgga cctcgacagg ctcgtcaccg catgctccgt cataatggat gcctcctttc
     1681 ggatgcataa catacgctcc ggctgtcatt ggcaccgtat cgtcgcagct acctgaacgt
     1741 ccttttccga acgcccatac tccggaaatc acagtgatgt gcctgtcttg atcatgataa
     1801 tgcggcggaa acgccagacc aggaggtatc ctgagacgaa tgacatacac gcccactttg
     1861 gcgggatctc ccgacaaaac cgcgtagcct agttccggag cctcgggatc cggctcatac
     1921 gacacctcgt ctggctggac gttgataaac gcggatgttg cttcgtccgc tagcgccgtt
     1981 gtcgccgggg aagccgcaag tgcccaggac atcagaaaca aaattctcgt ccgcacgttg
     2041 tctccaggaa taggaatggt gagtgtccgc tatggctcac tagcggaatt atgcctcagg
     2101 gtgcgggagg tcccggctac tcacgggaat ccagccattg ttcgagcctg gttttcagtt
     2161 ccagagccgc acgccgcatc ccgtcgagcc ccgcccggct gttgatggtc tggtacaaga
     2221 cttcgcctgc cgctatctcg agttccggga acgcccgcag ggctgccatc agctcctcca
     2281 ggtccggggc ctcggcggct tcgatcgcgt cggaggtgat gccgtgtccg aagctttcgg
     2341 ggccctgcag cacgcccagt cgtacgctgg gccccagcac attcgcgacg cgcgcgtcgt
     2401 actgagccca aatgcgatcg cgtatatccg cgagcggtat gctgatctcc tccaccgtcg
     2461 tgtagtaggc ggcgatcgcc ctccgcagtt ccgggtccct gatcaggcga aggttcccgg
     2521 tcgatcggag atcctcgagc gtgtttcgag tgatggacgg ggcatactgc cagccggcgg
     2581 tctccaccgc cgcaaccagg tcggcgacgt tgtccgtcgg cggcgatccc tcgtacaacg
     2641 cgagcacggt cgcagccgcg gcctcctgtc tgcgcatcca cgcttcggtc tcgtcgaatt
     2701 gagccagatc ggagtcaaga tcgatcacga gcgactccag gtactcgact tccagctggg
     2761 cgttgacgcg agcttcccgt agctgatcta cccagagcgc catcaggaca cccgccacga
     2821 tgacgacgaa ttcgccgagc agaaaacgcc aattaaaacg ggtcgacatc ttggtccgat
     2881 cctcagctgt catagttgga tagcaccgcc gcgcttattc caaggttttt ctgctggatc
     2941 agaagacgaa acccgcactt tcgatctgcg ccttgatctc ttgctccgtc tggccggagg
     3001 cgagcatgcg tgcctgttgc gctatctggt agcgttcctg ccgcgcatat tggcggaaag
     3061 gttcctcgcc atacaacgct tcgaataccg gcagttcgtc caccggcaag gcaatcgccg
     3121 agatggccaa tggcgccttg cctgcttcga tcaactgaag ttccgtcagc gcggtatcga
     3181 tgtcaccggc aaccgcatgt gccgcgaccc ggaccaggcg caaccggggc cggtagcgga
     3241 ccgcatcgtc ggacagcatc tctgacgtga gcaacaagcg ggtcaatcgg cccgactcgc
     3301 gctgcccgcc gttctccgca tagcagtgtg cgagcaagac ggcgtcgatg aggtcagtgg
     3361 cgtccaggta ttggagggtt tcgccctgca aggacgggaa ctgtcgttcg agcgacggta
     3421 cggcagcgtc acagcgaccg gcgatggcat cggcccaggc cgcgagcact gcgaaacggg
     3481 tggcaccgtt tcgttcgaac aatatccggg cttctgcggc tatcgcatcg aactgcctgt
     3541 cgagctgcgc ctgcacaagg cgtgaaatat ccacgccgcc gctggattgg ttgtagcgcg
     3601 cagcggcatc cagggcctcg tccgcccagc cgaacgccgt ataggagata agcgcgaacg
     3661 tgatctcgaa cggattcgga tcgagctcga tcgcccgttc catccagtac gtactcttgt
     3721 cggcacgtcc cagctcgtct tccatgccgg acaggtagcg atagatcatg ccgagctcgg
     3781 ggaacagcgc gcgcatcctg tacaggtgca cgcgtccctc gtcgtaacgt ccgagcccga
     3841 acagggcgtt cgacagacct gtcagcgcca gtgtattgag cggatcgagc tccaccaccc
     3901 gctcgaacat ttcgcgtgat ttgtccgcct ggttgatatc gagatagatg gacgcgagcc
     3961 attgaagggc caggatgtta ttgggatcct tcgcgacggc attctcgtaa tagcgcagcg
     4021 cccgttcctt ggcagcggtg tcgctggccg tgattacgcg gagttcttcg accagcccgg
     4081 ccgccgcgag tgtgtccgcg gtgggctccc cgagcttcag ggcccgat
//
LOCUS       DASUOM010000508         5291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_62285,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000508 DASUOM010000000
VERSION     DASUOM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62285"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VFY27_10600"
     CDS             complement(<1..1486)
                     /locus_tag="VFY27_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX5766006.1"
                     /translation="MNDNEERHDVAKDFIRQIISQDVASGKHGGAVVTRFPPEPNGYL
                     HIGHAKSICLNFGVAEEHGGCTYLRFDDTNPGKEKEEYVTAIKDDVRWLGYQWGDRLT
                     HASDYFDRLYESAERLIEMDKAYVDSLSAEEIREYRGTLTSPGRNSPFRDRRIDENLD
                     MFRRMRDGEFPDGAHVLRAKIDMASPNINMRDPTLYRIRHVPHQRTGDEWRIYPMYDF
                     AHGLSDAFEGITHSLCTLEFEDHRPLYNWFLEQLKPPHRPRQIEFSRLRLAFTVTSKR
                     RLNQLVEEGVVTGWNDPRMPTLAGMRRRGYPAAALRDFCTRIGVTKKDNVIEMSLLEN
                     CVRENLDNGALRRMAVLRPLKVVLTNYPEEVSEDVTAVNHPNNPELGTRQMSFGRELY
                     IEKSDFMEDPPKKFFRLRPGGEVRLRFAYIIRCDEVVRDDDGNVVELRCTYDPDTRSG
                     SGSSDKKVKGTIHWASAADAVAAEVRLYDRLFTHPEPGSSDAFLS"
     gene            complement(1559..2053)
                     /locus_tag="VFY27_10605"
     CDS             complement(1559..2053)
                     /locus_tag="VFY27_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5766007.1"
                     /translation="MNRTSAATAFPPAHHDHENCVEAALERAEVVCRERSLRLTGLRR
                     RVLELVWGSHKPAGAYEILDSLNREGRKAAPPTVYRALEFLIEADLVHRLDSLNAFVG
                     CSDPSSSHTGQFLICRQCRSVAELDDPEIDALVGEKAAVLGFTAVRQMVEIQGICRDC
                     RHSQ"
     gene            2293..4215
                     /gene="thrS"
                     /locus_tag="VFY27_10610"
     CDS             2293..4215
                     /gene="thrS"
                     /locus_tag="VFY27_10610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5766008.1"
                     /translation="MPNIKLPDGSVRQFDGAVSGLEIAARIGKGLARDAIAIRVNGAL
                     WDLTREIEDDASVAIITRDSEEGLELLRHDAAHVLAEAVKELWPETQVTIGPAIENGF
                     YYDFAREEPFTPEDLVAIEERMQQVVDRDEPIQREVWKRDEAVAFFRAQGEEYKAQII
                     EDIPVDETLTLYRQGNFVDLCRGPHLPSTGRLGKAFHLTRVSGAYWRGDARNPMLQRI
                     YGTAWATDKQLRQYLEQLEEAEKRDHRRLGKTMDLFHFQEEAPGAVFWHPKGWALFQK
                     LVKYMRQRQTEAGYQEVNTPELMDRSLWEASGHWESFGENMFTTDTVDGRSYAIKPMN
                     CPGHVQLFKQGLTSYRELPMRLAEFGKVHRYEPSGALHGMLRVRAFTQDDAHIFCTAE
                     QITDETTAVCDLILRIYRDLGFDDVKIKFADRPAIRVGADEVWDAAEAALLAALEKSG
                     LDYAHNPGEGAFYGPKLEFVLRDAIGRDWQCGTHQVDLNLPGRLGASYIGEDGQKHTP
                     VLLHRAIFGSLERFIGILLEHHAGNLPLWLSPTQAKVLTITSDADGYAMDVVSKLRSA
                     GIDAEADLRNEKISYKVREHSVAKVPVLMAVGQREVEEQTVALRRLGSRDQQVVKLDE
                     AIGRLRDEIRLRGRAA"
     gene            4470..>5291
                     /locus_tag="VFY27_10615"
     CDS             4470..>5291
                     /locus_tag="VFY27_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766009.1"
                     /translation="MALEHFKTQVLLLHSQQSTLDALSAGFGDRYAVHLATTGTEALS
                     TLGATPIHVIVSAQDLPGMSGLEALREAKKRSPDTIGILLAGNDPEDGLEALVGDKEV
                     FQIVRGSVTTDTLQNLIDTATKRVRMLTISESANDQAANVDEPVEDPASEHIVMETSE
                     NGTAIISDGTGRMPALRPRHVHIAAHAGGRDVDVLILTKDEDFLETIRDSSHESHTVH
                     HANTPAQAEAIVRDHNVGVLVTDAAMVGSNIELVTERLRKDRPRLVAVVAGRRDDG"
BASE COUNT         1101 a         1590 c         1587 g         1013 t
ORIGIN      
        1 gcgagaggaa ggcgtcggac gagcctggct cgggatgggt gaacaacctg tcatatagcc
       61 gcacttcggc agcgacggcg tccgcggctg atgcccaatg gatcgtaccc ttcacttttt
      121 tgtcgctgct gccgctcccg ctcctggtgt ctggatcgta ggtgcaccgg agctcgacca
      181 cgttcccatc gtcgtccctg actacttcat cgcagcgaat gatgtacgca aaacgaagcc
      241 gcacttcgcc accaggcctc aagcggaaga acttcttcgg aggatcttcc atgaagtcgg
      301 atttctcgat gtaaagctct ctgccaaagc tcatctgccg tgtccccagt tcgggattat
      361 ttgggtggtt gacggctgtt acatcctcac taacttcctc ggggtaattg gtcagcacga
      421 ccttcagcgg ccgaagaacc gccatccgtc gcaatgcccc gttgtcgaga ttctcgcgca
      481 cgcagttctc gagcagcgac atttcgatga cattgtcttt cttcgttacg ccaattcgcg
      541 tgcaaaaatc tctcaacgca gcagcgggat aaccgcgccg tcgcattcct gcaagcgtcg
      601 gcatgcgagg atcgttccag cctgtgacaa cgccctcctc caccagctgg ttcagtctcc
      661 gcttgctggt aactgtgaat gccaggcgaa gtctcgaaaa ctctatctgc cggggacgat
      721 gcgggggctt cagctgctcg agaaaccagt tgtacagcgg ccgatgatct tcaaactcga
      781 gggtgcagag tgaatgcgtg attccttcaa acgcatccga caaaccatga gcgaaatcgt
      841 acatcggata gatgcgccat tcgtcgccgg tacgttgatg aggtacatgc cgaatgcggt
      901 agagcgtcgg atcacgcatg ttgatgttcg gcgaggccat atcaatcttt gcgcgtagca
      961 cgtgtgcccc atccggaaac tccccgtcgc gcatgcggcg gaacatatcc agattctcgt
     1021 cgatgcggcg gtcacggaac ggactgttgc ggccgggcga cgttagcgtg cctcgatatt
     1081 cccttatttc ttcggcagaa aggctgtcta cataggcctt gtccatctca atcaagcgtt
     1141 ctgctgactc gtagaggcga tcgaaatagt cggacgcatg agtgagacgg tcaccccact
     1201 gataacccag ccatctcacg tcatctttta ttgcggttac gtattcttcc ttctctttgc
     1261 cggggttcgt atcatcgaat cgtagatagg tacaaccacc atgctcctct gcaaccccga
     1321 aattgagaca aatcgacttg gcgtggccga tgtgcaagta cccgttaggc tcgggtggaa
     1381 atcgcgtcac gacagctcca ccgtgcttgc cgctggcgac atcctgactg attatctggc
     1441 ggatgaagtc cttggcgaca tcgtgccttt cttcattgtc gttcatagat tccatttgat
     1501 gcgcaaaaag cgcgattcca cgtgatttag tgatggttct tggtgcccag cgttttgctt
     1561 attgtgaatg gcggcagtcg cggcaaatcc cctggatctc taccatctgg cggactgccg
     1621 taaagccaag cactgctgcc ttttcgccga ccaaagcatc gatctcagga tcatccagct
     1681 ctgccacgga gcggcactgg cgacaaatca ggaattgccc ggtatgggag ctactgggat
     1741 cactacagcc tacgaatgca ttcagtgagt ccagccggtg cactaggtca gcctcgatca
     1801 ggaattccag ggccctgtac accgtcggtg gcgcggcctt cctgccctcg cgattaaggc
     1861 tgtccagaat ctcgtaggca cctgccggct tgtgactgcc ccagaccagc tcaagcactc
     1921 gccgccgcag cccggtcagg cgcaagcttc tttcgcggca gactacttca gcacgctcga
     1981 gcgcagcctc gacgcagttc tcgtggtcat gatgtgcagg agggaatgca gttgcggcag
     2041 atgtacggtt catggcggca cgcggaggtt ctggctgcaa ttgtacacga ggcggcttgg
     2101 catcagatca gtaggttcgg gacatccatc aatcggcttt gtcctgagct tcctcaaggc
     2161 tagacgtctc cgtcgatcgg ggttagtggg ctgatcaagg tccggttaaa cgatggtgct
     2221 tccatttggt ggatgtcccg ttaaggagta attagtacaa tccgcaggtt ttcagcagct
     2281 gaagctcgga aaatgccgaa catcaagtta ccggacggga gcgtgcgtca gtttgacggc
     2341 gctgtgagtg gactggagat tgccgcgagg atcggcaagg gcctggcgcg ggacgcgata
     2401 gccatacgtg tgaacggtgc gctgtgggac ctgacgcggg agatcgagga tgacgccagc
     2461 gtcgccatca tcacgcgtga ttccgaggag ggactcgagc tgttgcggca cgatgcggcg
     2521 cacgtgctgg cggaagcggt caaggagctg tggccggaga cgcaggtgac gatcgggccc
     2581 gcgatagaaa atggcttcta ctacgatttc gcgcgcgagg aaccgttcac gccggaagac
     2641 ctggtggcca tcgaagaacg gatgcagcag gtcgtggacc gcgacgagcc catccaacgt
     2701 gaagtctgga agagggacga ggcggtggcg ttcttccgcg cccagggcga ggagtacaag
     2761 gcccaaatca tcgaagacat tccggtggac gagaccctga cgctctaccg tcaaggaaac
     2821 ttcgtcgacc tgtgccgcgg cccgcatctt ccctctaccg gcagactggg caaggcgttc
     2881 cacctgacgc gggtgtccgg cgcgtactgg cgcggcgacg cgcgcaaccc gatgctgcag
     2941 cgcatctacg gaaccgcctg ggcaaccgac aagcagctgc ggcagtacct cgagcagctg
     3001 gaagaagcgg aaaagcggga ccaccgtcgt ctcggcaaaa cgatggacct cttccacttc
     3061 caggaagagg ctcctggcgc ggtgttctgg cacccgaaag gatgggccct cttccagaaa
     3121 ctcgtaaagt acatgcggca acggcagaca gaagccggct accaggaggt caacacgcct
     3181 gaattgatgg accgctcgct gtgggaggcg tcgggccact gggagtcctt cggcgaaaac
     3241 atgttcacca ccgacaccgt cgacggcagg agctacgcga tcaagccgat gaactgccct
     3301 ggccacgtgc agctcttcaa acaaggcctg accagctacc gggagttgcc catgcgactc
     3361 gcggaattcg gcaaggtgca ccgctacgaa ccgtcgggag cgctccacgg catgctgcgc
     3421 gtgcgcgcct tcacccagga cgacgcgcac atcttctgca ccgcggagca gatcaccgac
     3481 gagacgaccg ccgtctgcga cctcatcctg cgcatttacc gggacttggg ctttgacgac
     3541 gtaaagatca agttcgcgga ccgccccgcc atccgcgtcg gcgccgacga ggtctgggat
     3601 gccgccgaag cagccttgct ggcggcactc gagaaatccg gcctcgacta tgcgcataac
     3661 cccggggaag gcgcgttcta cggccccaag ctggagttcg tcctgcgcga tgccatcggc
     3721 cgcgactggc aatgcggcac gcaccaggtg gacctgaacc tccccggtcg cctcggcgca
     3781 agctatatcg gcgaggacgg gcagaaacac acaccggtgc tgctgcatcg cgccatcttc
     3841 ggctcgctgg agcgcttcat cggcatcctg ctggaacatc acgcgggcaa cctgcctttg
     3901 tggctgtccc caacccaggc gaaggtgctg accataacct ccgatgcgga tggctacgcc
     3961 atggacgtcg tttcgaagct gcggagcgcc gggattgacg cggaagccga cctgcgcaac
     4021 gagaagatct cgtacaaggt gcgcgagcac agtgtcgcca aagtgccggt gctgatggcg
     4081 gtcgggcagc gcgaggtgga ggaacagacg gtcgcgctac gtcggctcgg ttcgcgcgac
     4141 cagcaggtcg tcaagctcga tgaagcgatc ggacgtttga gggacgagat ccgtttgcgc
     4201 ggccgcgcgg cctgaacgga aacagagtga cgaaaaaatg catcgccggg acatttacgt
     4261 tccgtatcgc tcctgcctat acttcccgtg gtccaacatc cgggacgtaa cgtgacgcct
     4321 ccgtcattcc aactgtgcag cccgtcacag attcaccatc ttgtcgggcc gcagcgacat
     4381 ttcacaatct gacgaggact atactcaccg ggcccaaggc tgtagccaga cggaaagcca
     4441 attgaacata tcccgccagg aggacagacg tggcgctgga gcactttaaa actcaagtcc
     4501 tgttgttgca cagccagcag agcacgctgg atgccctgag cgctggtttc ggcgacaggt
     4561 acgccgtgca tctcgcgacc accggcaccg aagcgctcag cacgcttggc gcaacgccta
     4621 tccacgtgat cgtttcggcg caggacctgc ctggcatgag cggcctggag gcgcttcgcg
     4681 aagcgaagaa acgctcgccc gacacgatcg gcatattgct ggcaggcaac gatcccgaag
     4741 acggcctcga ggcgctggtg ggcgacaagg aagtcttcca gatcgtccgc ggctccgtta
     4801 ccactgacac gctgcagaat ttgatagaca ccgcaaccaa gcgggtccgg atgctgacta
     4861 tctcggaatc cgccaacgac caggctgcaa acgtcgacga accggtcgaa gatccggcca
     4921 gcgaacacat cgtcatggag acgtccgaga acggcaccgc gatcatcagc gacggcaccg
     4981 gcaggatgcc ggctttgcgg ccgcgccacg tgcacatcgc ggcgcatgcc ggcgggcgag
     5041 acgtcgacgt actgatcctg accaaggacg aagattttct tgaaaccatc cgcgactctt
     5101 cgcacgagtc ccacacggtg caccacgcga acactccggc gcaggccgag gcaatcgtcc
     5161 gcgaccacaa cgtaggggta ctggtgacgg atgccgcgat ggtcggctcc aatatcgaac
     5221 tggtgacgga gcgcctgcgg aaggaccggc cgcggctggt ggcagtcgtc gcaggccggc
     5281 gcgacgacgg c
//
LOCUS       DASUOM010000509         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_752605,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000509 DASUOM010000000
VERSION     DASUOM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752605"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFY27_10620"
     CDS             <1..772
                     /locus_tag="VFY27_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5766010.1"
                     /translation="LLFSISIAGAFLRFHRFELVADDDVFRSTGGLLTRHEHSVNRSK
                     LQSLQASQHVALRIFRRFRLKARQASSGRAGKSSDFVVPICTGRELAELGRAFYQQEF
                     SGLTLDPLSSSFEGVSRHYVWSRVRMAGIAPAVVATAALWPSVGAAALIVLLWIPACA
                     FVVWQKYRHFGLMHGKDGLAFRSGLIGYRVVAWLHRKVQRVDITQSAFQRRKDVANLR
                     IFLAAGGGIRIPYLQYAKAKALRDYVLYRVESSREAWH"
     gene            complement(854..1633)
                     /locus_tag="VFY27_10625"
     CDS             complement(854..1633)
                     /locus_tag="VFY27_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766011.1"
                     /translation="MVRICGGIPVALLATSITLAAQGQEVSNLFESRLPWSGARQLVV
                     VTTPDWDSTRGEMRRFTRTGEGWEQVAGEVPVVIGREGAAWGVGMHPMQPGPEKQEGD
                     GRSPAGVFALGIAFGYAESANTRLSYVPMSASDWCIDVVDSPLYNQIVDARDVGLDAV
                     AGSTEPMRRDVHVDGDQRYKLGFVIEHNPANKPDAGSCIFAHLWSAADSPTAGCTAMD
                     EPHMRETLAWLELDANPVFILLPQQEYDRLRSAWHLPNLSE"
     gene            complement(1668..2924)
                     /gene="murA"
                     /locus_tag="VFY27_10630"
     CDS             complement(1668..2924)
                     /gene="murA"
                     /locus_tag="VFY27_10630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5766012.1"
                     /translation="MESITVTGGVKLNGRVTTSGSKNAALPVLFSALLADGVHRFDNV
                     PQLRDIDSTEQLLGALNCRTVRDGHSLEVHVVTPQDPLAPYDLVRKMRASILCLGPLL
                     ARYGEARVSLPGGCAIGSRPIDLHVDTMQRLGADINIDAGYVVARAKKLIGAHILFER
                     VTVGGTENALMAATLARGTTLIENAAKEPEVVDLARYLKAMGAKIEGEGTSVISIEGV
                     DELSPGRHRIVPDRIEAGTLLLAGAITGGQVTVDNCEPSHLLALFDKLQESGFSIRTE
                     GGSVTVEAADQWTGSDVATAPYPGFPTDLQAQFMALMTQAQGTSVITEGIFENRFMHV
                     QELVRLGANITPMTQVSVVRGRRGGLSGASVMATDLRASASLVLAGLAAKGETTVNRI
                     YHLDRGYERLERKLAELGANIQRVAQ"
     gene            complement(2998..>3913)
                     /locus_tag="VFY27_10635"
     CDS             complement(2998..>3913)
                     /locus_tag="VFY27_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766013.1"
                     /translation="GLNEHQQAWFVGIRSTFYRVAMITGQGVLVMVAGYLETATSSIS
                     ASWGITFFLLAAVFVLLFAWHRFSLPRPPGDIARDVDSLGTLLRESVATFASFFRRER
                     IGRILAFLLLYRFAEAQLVKLAAPFMLDGQEIGGLEMSTGAVGVAYGTVGVLMLTSGG
                     ILGGFLAARNGLRFWLAWMVAAINLPNAVYIFLAFVQPESLLLISIAVGIEQFGYGFG
                     FTAYLLYMIYIARGPHSTAYYAICTGFMSLGMMLPGMFSGWLQELLGYQQFFIWALAA
                     TIPSFVVVMLIPLDPEFGKRDTTPSATT"
BASE COUNT          756 a         1201 c         1205 g          751 t
ORIGIN      
        1 gctgctgttc tcgatctcga tcgccggtgc gttcctgcgc ttccatcgct tcgaactcgt
       61 tgccgacgac gatgtctttc gttcgacggg agggctgctg acgcgccacg aacattcggt
      121 gaaccgctcg aagctccagt cgttacaggc gagccagcat gtggcgctga gaatcttcag
      181 gcgttttcgg ctgaaggcca ggcaagcatc gagcggcagg gccggaaaaa gttccgattt
      241 cgtcgtgccg atctgcacgg gcagagaact ggcggaactc gggcgcgcgt tttaccagca
      301 ggaattcagc ggcctgacac tcgatccgct gtcgtcttcc tttgaaggcg tatcccgaca
      361 ttacgtctgg tcgcgcgtac ggatggccgg catcgccccg gccgtggtgg cgactgcggc
      421 gctgtggccg tcggtcggtg cggccgcgct catcgtcctg ctctggatac ctgcatgcgc
      481 cttcgtcgtg tggcagaaat accggcactt cggcctgatg cacgggaagg acggcctggc
      541 gtttcgcagc ggtttaatcg gctatcgtgt ggtggcgtgg ctgcatcgca aggtacaacg
      601 cgtggacatt acgcagtcgg cattccagag gcgcaaggac gtggcgaatt tgaggatatt
      661 cctggcggcg ggtggcggga tcaggattcc ctacctgcag tatgcgaagg caaaggcgtt
      721 gcgggattac gtcctgtacc gggtggagtc gagccgggag gcctggcatt agatgagctg
      781 tggtagcccg ccatggcgag tgcgagaggt gtccaagcac agcaagctcg gtcgcatgcc
      841 aggctcctac ggactattcg gagaggttag ggaggtgcca tgcgcttcgc aggcggtcgt
      901 attcctgttg cggcaggagg atgaagacgg gattggcgtc cagctccagc cacgcaagcg
      961 tttcccgcat gtgaggctcg tccatcgcgg tgcagccggc tgtcggagaa tcggcggcgc
     1021 tccacaagtg cgcgaagatg cagctgccgg catctggttt gttggctgga ttgtgctcga
     1081 ttacgaagcc gagcttgtag cgctggtcgc catcgacgtg cacgtcgcgg cgcatcggct
     1141 cggtcgagcc tgcgaccgcg tcaaggccga cgtcacgcgc atcgacgatc tggttgtaca
     1201 gcggcgaatc gacgacgtcg atgcaccagt cgctcgcact catcggaaca taggaaaggc
     1261 gcgtgttcgc ggattccgca taaccgaatg cgatgcccag cgcgaagact ccggcagggc
     1321 tgcgtccatc cccttcctgc ttttccggac ccggctgcat cggatgcatt ccaactcccc
     1381 atgcggcccc ctcccgacca atgacgacag gtacttcccc cgccacctgt tcccagccct
     1441 cgcccgttcg cgtaaacctg cgcatctcgc cgcgagtgga atcccagtca ggagtcgtga
     1501 caacgacaag ctgacgcgca ccggaccatg gcaggcggga ttcgaacagg ttggatacct
     1561 cctgcccttg tgctgcgagg gttatcgacg tggcgagcag cgccaccgga atccccccgc
     1621 atatgcgaac catcagcgtc atccatcgat catcggataa cccaatccta ctgagctacc
     1681 cgttgaatgt tcgcgcccag ctcggccagc ttccgctcga gcctctcgta accgcgatcg
     1741 aggtgataaa tgcggttcac cgtggtttcc cctttcgcgg cgagaccagc cagcacgaga
     1801 ctggcgcttg cccgaaggtc ggtcgccatc acgctggctc cactcagtcc cccgcgcctt
     1861 ccgcgaacga cagagacctg cgtcatcggc gtgatattcg cgccgaggcg caccagttcc
     1921 tgcacatgca tgaagcgatt ttcgaaaatg ccttccgtga tgaccgacgt tccctgcgcc
     1981 tgcgtcatca gcgccatgaa ctgtgcctgc aggtcggtcg gaaagccggg atatggcgcc
     2041 gttgcgacat cggagccggt ccactggtcc gccgcctcca ctgtcaccga tcctccttcg
     2101 gtgcggatcg aaaagccact ttcctgcagt ttgtcgaaaa gtgccagaag gtgcgagggt
     2161 tcgcaattat cgaccgtcac ctggccaccc gtgattgcgc ccgcaagcag caacgtgcca
     2221 gcctcgatgc ggtccgggac aatcctgtgg cgcccgggtg agagttcatc gactccttcg
     2281 atcgagatta cgctggtgcc ttcgccttcg atcttcgcgc ccattgcctt caggtatcgc
     2341 gcgagatcga cgacctcggg ctcctttgct gcgttctcta tcaacgtcgt gccgcgcgcg
     2401 agcgtagctg ccatcagcgc gttttccgtg cctccgacgg tgacgcgctc gaacaggatg
     2461 tgtgcgccga tgagtttttt cgcccgggcg acaacgtagc cggcatcgat gtttatgtcg
     2521 gcgccgagcc gctgcatcgt gtcgacgtgc aggtcgatgg gccgtgagcc tatggcgcaa
     2581 ccgccgggca ggctgacgcg agcttcgccg taacgtgcaa gcagcggccc gaggcacagg
     2641 atgctggcac gcatcttgcg aacaaggtcg tagggagcca gcggatcctg cggggtgacg
     2701 acatgtactt ccagcgagtg cccgtcgcgc accgtacggc agttgagggc gccaaggagc
     2761 tgttccgtcg agtcgatgtc gcgcagttgc ggcacattgt cgaaccggtg aacgccatcg
     2821 gccagcaggg cagaaaacag tacgggtaat gccgcgttct tggaaccgct ggtagtgacc
     2881 cggccattta gttttacgcc acctgttacc gttatcgatt ccaagctgtg atctcagttg
     2941 caggcgcctc gtaagcacct tcggcaattt tgcggtgcag gtgtggcgcc agtgcagtca
     3001 tgttgtggca gatggtgtcg tgtcgcgctt gccgaactcc ggatccaggg ggatcagcat
     3061 taccacaacg aacgaaggaa tggtcgcagc aagcgcccag atgaagaact gctggtatcc
     3121 caggagttcc tgcagccagc cgctgaacat acccggcaac atcatgccga gcgacatgaa
     3181 gccggtgcag attgcataat acgccgtgct gtgcggaccg cgcgcgatgt aaatcatata
     3241 gagcaggtaa gccgtgaagc cgaagccgta accgaattgc tcgatgccaa ctgcgatgct
     3301 tatcagcaag agactttccg gctgcacgaa tgccaggaaa atgtagacgg cattgggcag
     3361 gttgatcgct gcgaccatcc atgccagcca gaaccgcagg ccgttacgcg ccgccaggaa
     3421 tccacccagg atccccccgc tcgtaagcat cagcacgccg acggtaccgt aagcgacacc
     3481 aaccgcgcca gtgctcattt caagcccgcc gatctcctgc ccgtcaagca tgaacggagc
     3541 agcaagcttg acgagctgcg cttcggcgaa gcggtagagc aacaggaagg ccagtatccg
     3601 gccgatgcgt tccctgcgga agaatgaagc gaacgtcgcg accgattccc gcagcaaagt
     3661 ccccagcgaa tcgacatcac gcgctatgtc tcccggcggg cgcgggagac tgaatcggtg
     3721 ccacgcgaac aggaggacaa acacagctgc cagcaggaag aacgtgatgc cccacgaagc
     3781 gctgatactt gacgtggcgg tctcgagata acccgcgacc atgacgagga cgccctggcc
     3841 agttatcatc gccacccgat agaaggtgct gcggatgccg acgaaccagg cctgctgatg
     3901 ctcgttcagg cca
//
LOCUS       DASUOM010000510         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1633082,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000510 DASUOM010000000
VERSION     DASUOM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633082"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFY27_10640"
     CDS             complement(<1..1620)
                     /locus_tag="VFY27_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766014.1"
                     /translation="MASGDTISARTAGSRIIRLVAAVLLLGAGVSAQAQDCSDYPNGL
                     LDGFAGTIAPSQLQIDRNCSIRNYPASNPLGTNFSFLTQPGQTDERWIIIFDNVVHVG
                     QMACNAVAGHKIWFTNGSSSTIQENCQNLLIPVEKIDKQNPAGQATAAIGVPFTYRLT
                     MPVLFDPATGSVINSLGSLNDLHGVTVWDDLNATGVDLSYVSHAVYWEDSGAAVPHTF
                     SNVGGLLTFDNFPIIPAGDQIVLEITVVLDETPANAPGTQFVNTAKWDFGRLIDGEFF
                     EPLPGEWGITPPMTIVGPDLVLAKSGPATMNLGESGQFTLDIHNIGNGDAWNTTLLDR
                     LPDGPSGGMCDMAPEVQSARVLQADGVTPVPGKGAMVQGSDFSLAYNGAPACQLTLTV
                     LTPAGTIGANERLIVTYQTQLDANTQNGATLTNVAGATQWFNADAGSSNRQTYTRTLT
                     NGTVGTPDHEDAHTVTTALTGYFFEKSVANLTSGANPAATAAPGDTLRYTLRLRTTDT
                     PLADFRFYDDLGVLNSSAVFVPGSLTLVPGAV"
     gene            complement(1658..>2913)
                     /locus_tag="VFY27_10645"
     CDS             complement(1658..>2913)
                     /locus_tag="VFY27_10645"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5766015.1"
                     /translation="RVCGSLEELEQLGAGKLFWGQSGDLGNSAEHVRKVRQRVQKFAS
                     HVAGIEQRRQAIVGQIEVQNDSLGLLGDDLLDALEEEESRRAEWIIEREEGTVPHRVQ
                     VMPWMRGVEEDWRFRRSLTASMAFTMLLAFVIQLIDLPIPEREELIEVPERVAKLIEK
                     RQIPVPPQPVVEEAPREEAPPPEPEPLLAEEEPPKPSPEPVEEPAMQETKQASAKEQV
                     QTKGILAFRETFASRAQSRPSAQLGSQARLGNAGDAAIGRPERSMVATTGAGSSGGIN
                     LAQISRNVGGGGGQDIAGVEVTQVASAIGSGGGADRPLSGGQFAGRTDEEIQIVFDRY
                     KSSLYRLYNRELRNDPTIRGQIVIRLTIEPDGSVSLCQLESSNMGAPTLAEQVVDRIR
                     GFDFGAKEGIVAMTIIYPIDFLPAA"
BASE COUNT          449 a          908 c          967 g          589 t
ORIGIN      
        1 gacggcgccg ggtaccagcg tgagtgagcc cgggacaaaa acggcggagc tgttgagcac
       61 accgaggtcg tcgtagaacc tgaaatcggc gagcggcgtg tcggtggtcc gcaggcgcaa
      121 cgtgtagcgc aatgtgtcgc ccggagccgc cgttgccgcg gggttcgccc cgctagtgag
      181 attcgcgacg cttttttcga agaaatagcc agtgagcgcg gtcgtgacgg tgtgcgcgtc
      241 ttcgtgatcg ggcgtgccta ccgtgccgtt cgtcagggtg cgcgtgtagg tctggcgatt
      301 gctgctgccc gcgtcggcat tgaaccattg ggtggcgccg gcgacgttgg tgagtgtcgc
      361 gccgttctgt gtgttcgcat cgagctgtgt ctggtacgtg acgatcaggc gctcgttcgc
      421 accgatggta ccggcaggcg tcagcaccgt cagcgtgagc tggcaggccg gtgcgccgtt
      481 gtaggcgagc gagaagtcgg agccctggac catcgcgccc ttgcccggca ccggcgtcac
      541 gccatcggcc tggaggactc gcgcgctttg cacctccggc gccatatcgc acatgccgcc
      601 actcggaccg tccggaagcc gatctaggag cgtcgtgttc caggcatcgc cgttgccgat
      661 gttgtgaatg tcgagggtaa actggcccga ttcgccgaga ttcatcgtcg ccggaccact
      721 tttcgccagc acgaggtcgg gcccgacgat ggtcatgggc ggcgtgattc cccactcgcc
      781 agggagaggc tcgaaaaact cgccgtcgat gagccgcccg aagtcccact tggcggtgtt
      841 gacgaactgc gtcccaggtg cgttcgccgg cgtctcatcc agcacgaccg tgatttcgag
      901 cacgatctgg tcgccggccg ggatgatggg aaagttatcg aaggtgagca gtccgccgac
      961 gttggagaag gtatgcggta ccgcggctcc gctgtcctcc cagtacacgg catgactcac
     1021 gtaggaaaga tccacccccg tcgcgttcag atcgtcccag acggtgacgc cgtgcaggtc
     1081 gttcagcgaa ccgagtgagt tgatgacgct gcccgtcgcg gggtcgaaca ggaccggcat
     1141 ggtgagcctg tatgtgaacg gcaccccgat agcggccgtg gcctggccgg ccgggttctg
     1201 cttgtcgatt ttttctaccg gtatcaggag attctggcag ttctcctgga tcgtgctcga
     1261 ggagccgttg gtaaaccaga tcttgtgccc cgcaacggcg ttgcaggcca tctggccgac
     1321 gtgcacgacg ttgtcgaaga tgattatcca tcgttcgtcg gtctggccgg gctgggtcag
     1381 gaaactgaag ttcgtgccga gcgggttgga cgccgggtag ttcctgatcg agcagttcct
     1441 gtcgatctgg agttgcgagg gagcgatggt gccggcaaac ccgtccagca ggccgttcgg
     1501 gtaatcggag cagtcctgtg cctgcgccga gacgcctgca cccagcaaca gcacggcagc
     1561 gaccaaccgg atgatccggc tgcccgctgt ccttgcactg atggtgtcgc cagaagccat
     1621 gaaggtgtgc tcgcgtgcga tcgggccgcc cgctgaatca ggctgcgggc aggaagtcga
     1681 tcgggtagat gatggtcatc gccacgattc cttccttggc gccgaagtcg aagccgcgga
     1741 tgcgatccac tacctgctcg gcgagcgtcg gcgcgcccat gttcgacgac tcgagctggc
     1801 acagggagac gctgccgtcc ggctcgatcg tcaggcggat gacgatttgt ccacgaatcg
     1861 tcgggtcgtt gcgcagttcc cggttgtaga gccggtatag cgacgacttg tagcggtcga
     1921 atacgatctg gatttcctcg tccgtgcggc cggcgaattg tccgccgctc agcgggcgat
     1981 ccgctccgcc gccgctgccg atagcacttg cgacctgggt aacctcgacg cctgcgatgt
     2041 cctggcctcc cccgccgccg acattgcggc tgatttgcgc gaggttgatg cccccgctcg
     2101 agcccgcgcc tgtcgttgca accatcgagc gttccggccg tccgatggca gcatcaccgg
     2161 cgttgcccag ccgggcctgt gagcctagct gcgccgacgg ccggctttgc gcgcggctgg
     2221 cgaaggtttc cctgaaggcg agaatgccct tcgtctgtac ctgttccttg gcgctcgcct
     2281 gcttcgtttc ctgcatagcg ggctcctcca ccggctcggg gctgggcttc ggcggctcct
     2341 cctccgcgag cagcggttcg ggttcgggcg gcggtgcttc ttcccggggc gcttcttcga
     2401 cgacgggttg cggcggcacc gggatctgcc gcttttctat gagctttgca acgcgttccg
     2461 gtacctcgat gagttcctcg cgttccggaa tgggcagatc gatcagctgg ataacgaacg
     2521 ccagcagcat ggtgaacgcc atcgacgctg tgagcgatct gcggaaccgc cagtcttcct
     2581 cgacaccgcg catccagggc atcacctgga cacgatgcgg taccgttccc tcctcgcgct
     2641 cgattatcca ctcggcccgg cggctttctt cttcctccag ggcatcgagg aggtcgtcac
     2701 cgagcaggcc gaggctgtcg ttctgaactt cgatctggcc gacgatcgcc tgtcggcgct
     2761 gctcgatgcc tgcgacgtgc gaggcaaatt tctgcactcg ctggcggacc ttcctgacgt
     2821 gttccgcact gttgccgagg tcgcctgact ggccccagaa aagcttgccg gcgccgagct
     2881 gctcgagttc ttcgagcgag ccgcagacgc ggt
//
LOCUS       DASUOM010000511         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1757135,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000511 DASUOM010000000
VERSION     DASUOM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757135"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2707
                     /locus_tag="VFY27_10650"
     CDS             <1..2707
                     /locus_tag="VFY27_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766016.1"
                     /translation="KTNLANFSRKMQGQRDLHSVADMLLSELAPLVGAQQGIFYVASD
                     SMHGPRLKQVAAYADSAAPGFVREYAFNEGLIGQCAQERKSLVISDVPANSVRINSGL
                     LDAAPRSVVVLPALFEDKVRAVIELASISDFTKSHVSFLEQLADIIGVVINNIEGTMR
                     TERLLAQSQQLATEQQTQQRELQKTNEELAQKAQLLAAQNAEVERKNQEIEQARRALE
                     EKAAELALTSRYKSEFLANMSHELRTPLNSILVLGQQLADNAQNNLSARQVEYASTIR
                     AAGTDLLNLISDILDLSKIESGTVAVESEEVELAGLKKTVERTFRHESESSSLSFTTE
                     FESSLGSTINTDPKRLLQVLKNLLSNSFKFTERGSVRLRVSSAKKGWTPGHPILDKAR
                     SVIAFAVSDTGIGIPLDKQKIIFEAFQQADAGTARKHGGTGLGLAISRELAQLLGGEI
                     TLHSTPGSGSTFTLYLPASYAGPVLAVTRNATVDTAATAYAPMLSPSTGKTTVADDRQ
                     TLVHGDRVLLVVQKDPTIAGMLLDAARSAGMKVVVAARGADAAVLARKYRPEAVVLDT
                     FLVDMHGWSVLSRFKREPDLRHIPVQVIVDDADEREARARGAFATITKPVRPSDLRSN
                     LQRMLDFADAPKRHLLVAGGRAVAEGGIVEVAAGSDIETTVAAGPSDAMRLLQQREFD
                     CVVVDALQSDMAAVEFLQEAAAVPALRNLAFVIRADAGWAAKNRPQVDMLARSMVLRL
                     AFTIDGVLDECCLFLHRRVSMLPTHKQYMLRRAADYDILAGKTILIVDDDVRNIFALS
                     TLLERHNMQVVSSQTGRDALRLIGETEDLSLVLLDLMMPELDGIETMRQMRTNWKFER
                     PIIALTAKAMTGDREKCIEAGASDYIAKPVDSDNLLSLLRVWLNR"
     gene            2764..>3396
                     /locus_tag="VFY27_10655"
     CDS             2764..>3396
                     /locus_tag="VFY27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766017.1"
                     /translation="MTEQANILLVDDKPERLLTYEVILESLNQNLVRANSGEEAFEKL
                     MAMEFAAILLDVSMPGMDGFETAALVRSQPRFGATPIVFVTGVHITDLDRLRGYEMGA
                     ADYVYVPVVPEILRGKVQVLVQLYLQRRELARLNEQLAAANDELAEAHARLKAENTRE
                     LQKLNRTLEEANRQLVSEVGERKRAEALLREAARRKDEFISILGHELRNPL"
BASE COUNT          695 a         1071 c         1073 g          557 t
ORIGIN      
        1 gaagaccaat ctcgccaact tcagccgcaa aatgcagggc cagcgcgacc tccactcggt
       61 ggcggatatg ctgctttcgg aactggcgcc gctggtcggc gcacaacagg gcatattcta
      121 cgttgcgtcc gactccatgc atgggccgcg cctgaagcag gtcgccgctt acgcagacag
      181 cgccgctccc ggcttcgtgc gcgaatacgc cttcaacgaa ggcttgatcg gccagtgcgc
      241 gcaggagcgc aagtcgctcg tcatttcaga cgtcccggcg aactccgttc gcataaactc
      301 gggcctgctg gatgccgcgc cgcgcagcgt ggtcgtgctg cccgcattgt tcgaagacaa
      361 ggtccgggcc gtgatcgagc tggcatcgat cagcgatttc acgaagtctc acgtctcctt
      421 tctcgagcag ctcgccgaca tcatcggcgt ggtcatcaac aacatcgagg ggacgatgcg
      481 caccgagcgg ctgcttgccc agtcgcagca actggcgaca gagcagcaga cccagcagcg
      541 cgaactgcag aaaacgaacg aagaactggc gcagaaagcg caactgctgg cggcgcagaa
      601 cgccgaggtc gagcggaaga accaggaaat cgaacaggcg aggcgcgcac tggaggaaaa
      661 ggcggcggaa ctggcgctca cttcccgcta caagtccgag ttccttgcga acatgtctca
      721 cgagctgcgc acgccgctga atagtatcct cgtgctcgga cagcagctgg cggacaacgc
      781 gcaaaacaac ctgagcgcgc gacaggtcga gtacgcgtct accatccggg cggcgggcac
      841 cgatctgctg aaccttatca gcgatatcct ggacctgtcg aagatcgagt ccggcaccgt
      901 cgcggtagaa agcgaagagg tcgaacttgc cggtctcaag aagacggtgg agcgcacctt
      961 ccgccacgaa tcggaatcca gcagcctctc gttcacgacc gagttcgagt catctctcgg
     1021 cagcactatc aataccgacc cgaagcgcct gctgcaggtg ctgaagaacc tcctgtcgaa
     1081 ctcgttcaag ttcaccgagc gcggcagcgt ccgcctgcgg gtaagttcgg cgaaaaaagg
     1141 ctggacgccg ggacacccga ttctcgacaa ggcccggtcc gtcattgctt tcgcagtcag
     1201 cgacacgggc ataggcatcc cgctggacaa acagaagatc attttcgagg cgttccagca
     1261 ggcggatgcc ggcacggcgc gcaagcacgg cggcaccggc ctaggcctgg cgatcagccg
     1321 cgaacttgca caactgctgg gcggggagat aaccctgcac agcacgcctg gcagcggcag
     1381 tacgttcacg ctctaccttc ctgcgagcta cgccggcccc gtgttggcgg tgacgcggaa
     1441 cgcgacggtg gataccgcgg cgacggctta cgcaccaatg ctctcgccat cgacgggcaa
     1501 gactaccgtc gctgacgacc gccagaccct ggtgcatggc gaccgggtgc tcctggtggt
     1561 tcagaaggat ccgacgatcg ccggcatgct gctcgacgcc gcccgctccg ccggcatgaa
     1621 agtcgtggtc gcggcacggg gagccgatgc agccgtactg gcgcgaaagt accgtcccga
     1681 ggcggtggtc ctcgacacct tccttgtcga catgcatggc tggtcggtgt tgagccggtt
     1741 caagcgcgag ccggacctgc ggcacattcc ggtgcaggtg atcgtagacg acgccgacga
     1801 gcgcgaggcg agggcccgcg gtgcgttcgc gaccatcacc aaaccggtgc gcccttccga
     1861 cctgcgtagc aaccttcaaa ggatgctcga ctttgccgac gcaccgaagc gccacctgct
     1921 ggttgccgga ggccgtgctg tcgcggaagg cggcatcgtc gaggtggccg cggggagcga
     1981 catcgaaaca acggtcgccg cgggaccgtc cgatgcgatg cggctgttgc aacagcggga
     2041 gttcgactgc gtcgtcgtcg acgcgctgca gtccgacatg gccgcggtgg aattcctgca
     2101 ggaggcggcg gcagtgccgg cgctgcggaa cctggctttt gtcattcgcg ccgacgccgg
     2161 gtgggcggcg aagaaccgcc cccaggtcga catgctcgcc cgctccatgg tccttagact
     2221 ggcatttacg atcgacggcg tcctcgacga atgctgcctg ttcctgcacc ggcgggtctc
     2281 gatgttgccg actcacaagc agtacatgct gcgccgggca gccgattacg acatactcgc
     2341 gggcaagacc atcctgattg tcgatgacga tgtgcggaac atcttcgcac tgtcgaccct
     2401 gctggagcgc cacaacatgc aggtagtgtc gtcccagacg ggacgggatg cactgcgcct
     2461 gatcggggaa acggaggacc tgtcgctggt cctgctcgac ctgatgatgc cggagctcga
     2521 cggtatcgag acgatgcggc agatgcgcac gaactggaag ttcgaacggc ccattatcgc
     2581 cctcacggcg aaggcgatga ccggcgatcg cgagaagtgc atcgaagccg gggcatcgga
     2641 ctacatcgcg aaacccgtcg atagcgacaa ccttctgtcc ctgcttcgcg tctggctgaa
     2701 ccgctgacgc acagcaaccg caccgattct tatcaacatg gaaacgcccg cgatgcgccc
     2761 gacatgacgg aacaagcgaa tatcctgctg gtcgacgaca agcccgagcg cctgctgacc
     2821 tacgaagtca tcctggaaag cctgaaccag aacctcgtac gtgcgaattc cggcgaggaa
     2881 gccttcgaga agctcatggc gatggagttc gcggcgatcc tgctggacgt cagcatgccc
     2941 ggaatggacg gctttgagac cgccgcgctg gtacgcagcc agccacgctt cggcgccacg
     3001 ccgatcgtgt tcgtgaccgg cgtgcacatc acggacctcg atcgcctgcg cggctatgaa
     3061 atgggagctg ccgactacgt ttacgtcccg gtagtgccgg aaatacttcg cggcaaggtg
     3121 caggtactgg tacagcttta cctgcagcgg cgtgaactcg cgagactgaa cgagcagctg
     3181 gccgccgcca acgatgagct tgctgaggcg catgccaggc tgaaagcgga gaacacccgc
     3241 gagttgcaga aactgaatcg tacgctcgaa gaggcaaacc gccagctggt ctccgaggtt
     3301 ggcgaacgaa agcgcgccga ggccctgctc agggaagcgg cccggcgcaa ggacgagttc
     3361 atatcgatcc tcgggcacga attgcgcaat ccactg
//
LOCUS       DASUOM010000512         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1500441,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000512 DASUOM010000000
VERSION     DASUOM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500441"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFY27_10660"
     CDS             <1..842
                     /locus_tag="VFY27_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5766018.1"
                     /translation="AIAVAQEATNLARDIETGNIDTESTLLWLEQRIPFLPELIERFQ
                     VDLARIQEGLAGAAIAAGRWVAGNAVTIGQNALQFFLRLFVMLYLLFFFIKDGKSILL
                     AVTRVLPLDMELQQNLKSRFANVARATLKGTFLISAIQGLIGGISFWALDIRAPILWG
                     VVMAVFALIPAVGPAIVWLPAAIILLASGEVAKGLILIVIGAVVIGLVDNLLRPVLVG
                     RDARLPDYIILLTTLGGITVFGLSGVLIGPIIAALFFTVWTSLGDRQQPEGEAPAGSL
                     NRP"
     gene            870..1658
                     /locus_tag="VFY27_10665"
     CDS             870..1658
                     /locus_tag="VFY27_10665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5766019.1"
                     /translation="MFPIRDENPQITTPLVTYAIIVLNVAAWLLLQGLGSEPALTQSV
                     CQLGLIPGDLLGTIDRAQVPPDFPCSIDGQSNWLTMLTSMFMHGGWMHIIGNMWFLWI
                     FGGNVEDSMGHLKFAIFYVLCGIAAGAAQVFANTASPIPMVGASGAIGGVMGAYIVLY
                     PRVHVHMLLLLGFYITTFAVPAVWMLGYWILVQFVSGLTSIGAQGGGVAFWAHVGGFA
                     AGAALVFLFKDESLLARHPYRGWHQKKHATQAWHKARDDWRRRR"
     gene            1640..3445
                     /gene="aceK"
                     /locus_tag="VFY27_10670"
     CDS             1640..3445
                     /gene="aceK"
                     /locus_tag="VFY27_10670"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX5766020.1"
                     /translation="MAPQALNGPPARGHPPTGNGEGRRERQAASRLADSFSNYNRRFR
                     ELTRRTGRHFLNRDWGAARSDAVRRIDLYNDSVTATMHEMRTLLGEKIASREAWGRIK
                     NAYAQIIADRPDSEFYKTFFSSVTRRNFDTVGVDPLVEFLAEDVEPSTGDRYPLQTRV
                     YRNRGSLFYLFDEILGDLPFAHRFRDVEGTIAFVTAEIGAWRNEQKIESYPREVEVIP
                     PLFYRGRRAYLVGRVLGEGWRVPLVIPFANPQDGITSDTVILSEAEVSMLFGFTRSYF
                     HVDLEVVGSAVRFLRELMPLKPVEEIYTVLGRAKQGKTERYRSLMRHMKNSTDLFRFA
                     PGAAGMVMQVFTLSSFNVVFKVIRDKFGFPKNILPEQVREKYELVFKHDRAGRLVDAQ
                     EYRRLSFDLDRFEPKLLESLLGECSRNCSIQGEKLLIEHLYIERRLVPLNLFLKDAPA
                     DDAARAVIDYGQAVRDLASSNIFAGDLLMKNFGVTRHGRVIFYDYDEVTFVTNCNFRP
                     LPESDYAEDEMSAEPWFFVGPNDVFPEEFIRFFGFNRELQEVFRRYHSELLSHEWWSG
                     IKRRLEQGDIIEVLPYSVRSRTTKLAGAGLYAGRT"
BASE COUNT          651 a         1031 c         1099 g          779 t
ORIGIN      
        1 ttgcgatcgc ggtagcacag gaagcgacca atctcgcacg ggacatcgaa accggtaata
       61 tagatacgga atccactctg ttgtggctcg agcagcgtat cccgttcctg cctgaattga
      121 tcgagcgatt ccaggtcgat cttgcacgca tccaggaagg ccttgccggt gccgccatag
      181 ccgcgggccg gtgggtggcc ggaaacgcgg tcacgatcgg gcaaaatgcg ctgcaatttt
      241 tcctgcgcct tttcgtaatg ctttacctgc tgttcttctt catcaaggac ggcaagtcga
      301 tcctgctggc cgttacgcgg gttctccccc tggacatgga actgcagcag aatctgaagt
      361 cgcgattcgc gaacgtcgcg cgagccaccc tgaagggcac cttcctcatc agtgcgatcc
      421 agggcttgat tggcgggatc tcgttctggg ccctcgatat tcgcgcgccg atcctgtggg
      481 gagtggtcat ggcggtgttc gctctcattc cggccgtcgg gcccgcgatt gtatggctgc
      541 cggccgctat catactgctg gcctccggtg aagtggccaa gggactgata ctgatcgtca
      601 tcggtgcggt cgtgatcggg ctggtcgaca acctgctgcg cccggtcctg gtggggcgcg
      661 atgcccgcct gccggattac atcatcctgt tgactacgct tggcggaatc acggtcttcg
      721 ggctgtccgg cgttcttatt ggtcccatta tcgcagcgct gttctttacc gtctggacca
      781 gcctcggaga ccggcaacag cccgaaggtg aggcacccgc cggcagcttg aaccgacctt
      841 agcactccaa gccgactggc aggccgccga tgttcccgat cagagatgaa aatccgcaga
      901 ttacgacacc gctcgtaacc tacgcgatca tcgtcctcaa tgtcgccgcc tggctgctgt
      961 tgcaggggct gggcagcgag cctgcgctga cgcagtcggt gtgccagctc ggcctgattc
     1021 ccggcgacct gctcggcacg atcgaccgcg cccaggttcc tcccgacttt ccctgctcca
     1081 tcgacgggca gtcgaactgg ctgacgatgc tcacatcgat gttcatgcat ggcggctgga
     1141 tgcatatcat cggcaacatg tggttcctct ggatcttcgg cggcaacgtg gaagattcga
     1201 tggggcacct gaagttcgca atcttctatg ttctctgcgg gattgcagca ggcgccgcac
     1261 aggttttcgc gaacacggcc tcacccatac cgatggtcgg tgcgtccggc gccatcggcg
     1321 gggtgatggg ggcttacatc gtcttgtatc cccgcgttca tgtgcacatg ctcctcctgc
     1381 tcggctttta cattactacg ttcgcagttc cggcagtctg gatgctcggt tactggatcc
     1441 tggtgcagtt cgtcagcggg ctgacatcga ttggcgccca ggggggcgga gtagcatttt
     1501 gggcgcacgt gggcgggttt gccgccggcg cggcgctggt attcctgttc aaggacgagt
     1561 cgctgctggc tcgtcatccg tatcgcggct ggcaccagaa aaagcatgcg actcaggcgt
     1621 ggcacaaggc acgggacgat tggcgccgca ggcgctgaac gggccgccgg cgcgcgggca
     1681 tccgcccacc ggtaatggcg aggggcgaag ggaacggcag gccgcttcga gacttgctga
     1741 ctcctttagt aactacaacc gccgctttcg cgagcttacc cgccgcaccg gtcgccactt
     1801 tctcaacagg gattggggcg cggcgcggtc ggacgcggtc aggcggatcg acctctacaa
     1861 cgattcggtc acggccacca tgcatgagat gcggacgctg ctcggagaaa aaattgccag
     1921 ccgggaggcg tggggccgca tcaagaacgc ctacgcgcaa attattgcgg atcgtccgga
     1981 ttcggaattc tacaagacgt ttttcagttc cgtcacgcgg cgaaactttg acaccgtcgg
     2041 agtcgacccg ctggtggagt ttcttgcgga ggacgttgag ccgtccaccg gcgacaggta
     2101 tccgttgcag acccgcgttt accggaaccg cggctcgctg ttctaccttt tcgacgaaat
     2161 cctgggagat ttgccgtttg cgcaccggtt tcgcgacgtc gagggtacga ttgcattcgt
     2221 taccgcggaa atcggcgcgt ggcggaacga gcagaaaatc gaatcctacc cgcgggaagt
     2281 agaggtgata ccgcctttgt tctaccgtgg gcgccgcgcc tacctcgtcg gccgagtgct
     2341 tggtgaaggt tggcgggtgc cgctcgtcat cccgtttgcc aacccgcagg atggtatcac
     2401 ttcggacacg gtgattcttt ctgaagcgga agtcagcatg ctgttcggct tcacgcgaag
     2461 ttatttccat gtcgacctgg aagtcgtggg cagcgcggtg cgcttcctgc gtgaactaat
     2521 gccgctgaag cccgttgagg aaatctatac tgtgctcggg cgtgccaagc agggaaagac
     2581 ggagcgttac cgctccctga tgcggcacat gaaaaactca acggacctgt tccggttcgc
     2641 ccccggcgcg gccggcatgg tgatgcaggt atttacgctg tcgtcgttca acgtcgtatt
     2701 caaggtaata cgcgacaagt ttggctttcc aaagaacatc cttcccgagc aggtaaggga
     2761 gaagtacgaa ctggtgttca agcacgatag agccggaagg ctggtggatg cgcaggaata
     2821 ccgtcggctg tcattcgacc tggatcgctt cgagccgaaa ctcctggaat ccctgctcgg
     2881 ggaatgcagc cggaattgtt cgatacaggg agaaaagctc ctcatcgaac acctgtatat
     2941 agagcgccgg ctggttccgc tgaacctgtt cctcaaggat gctccggcag atgacgcggc
     3001 tcgcgccgtt atagactacg ggcaggcggt gcgggacctc gcctcgagca acatctttgc
     3061 cggcgacctg ctgatgaaaa acttcggcgt tacccgccat ggacgcgtca tcttctacga
     3121 ttacgacgag gttacgttcg tcaccaactg caacttccgg cctctgccgg agagcgacta
     3181 cgcggaggac gagatgtccg ccgagccatg gttttttgtc ggcccgaatg atgtatttcc
     3241 cgaggagttc atcaggttct ttggtttcaa cagggagctg caggaggtat ttcgccggta
     3301 tcactccgag ctgctctcgc atgagtggtg gtccggtatc aagcggcggc tcgaacaggg
     3361 cgacatcatt gaagtgctcc cgtatagcgt gaggagtcgt accaccaagc ttgccggggc
     3421 gggcctgtac gccggcagaa cgtgacagga gtaccagatg gatctctctg aatggctcga
     3481 ctaccaggtt ctgggtaatt ctctgctggc ctgggcgtcg ggattggcgc tttttgtcgc
     3541 cgcctggatc gtcctcaagt
//
LOCUS       DASUOM010000513         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_938982,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000513 DASUOM010000000
VERSION     DASUOM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938982"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFY27_10675"
     CDS             <1..224
                     /locus_tag="VFY27_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5766021.1"
                     /translation="RVYEGKVARLMDFGAFVTILPGKDGLVHISQISNERVEKVSDKL
                     AEGDVVKVKVLEVDRQGRVRLSMKEVEAA"
     gene            complement(283..1506)
                     /locus_tag="VFY27_10680"
     CDS             complement(283..1506)
                     /locus_tag="VFY27_10680"
                     /inference="COORDINATES: protein motif:HMM:NF008218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX5766022.1"
                     /translation="MHCSRDTLPRPLLQKPEGVNTPRPAPCASRGPFFCPRLPASHHP
                     CSRVPCSRNFQKNRAPPVNTGQPAGRIRQSDFRPAAWLRNRHMQTVFPSLPLLRLPRP
                     PLYREMLELPDGDVTVVDWLEEVPRTTETSPLLVVLHGLESSAKSTYARQLLHAAAGR
                     GWRAAVLHFRDCGDYRNRLPRRYHAGETSDLRYFLHKLRTDGHEGPLLAAGYSLGGNV
                     LLKYLGESAADTPLLAAAAVSVPLNLHESSKALSQGISRVYQNYLLRRMKLSVTRTFN
                     PHTAAFDWGKAMGAATFAEFDDAVTAPLHGFAGKDDYYDRCSSIRFLKTIERPTLIIN
                     ALDDPFMTPDVIPDESQVSDFVTLEVSEQGGHVGFIDGGTPWRPQYYLPRRITRFLEG
                     ELAEQETEMVAIPGL"
     gene            complement(1732..2121)
                     /gene="phaR"
                     /locus_tag="VFY27_10685"
     CDS             complement(1732..2121)
                     /gene="phaR"
                     /locus_tag="VFY27_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX5766023.1"
                     /translation="MSTRRVINKYPNRRLYDTEESRYITLSDIRDLVLERVDFAVIDK
                     KDGSDITRCILLQVISEREQQEDPVLSQRFLLQIIRSCDGRAALPLSSHLEQSLSAFL
                     QNSGRSGTAPATGTWKPQLTAGRKKVG"
     gene            complement(2168..3106)
                     /gene="gluQRS"
                     /locus_tag="VFY27_10690"
     CDS             complement(2168..3106)
                     /gene="gluQRS"
                     /locus_tag="VFY27_10690"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX5766024.1"
                     /translation="MRNASSGIGTTVTGKQYIGRFAPSPTGPLHFGSLVAAAASYLQA
                     RSCRGRWLLRIEDIDPPRERRGAADAIVSALETYGFEWDGPVLYQSRNNAQHRAAVER
                     LLDEWRAYPCGCSRQRLATAPQGPLGRIYPGTCRAGSNSRHSAIRLRTDNEPVGFVDL
                     VQGPQEQRLESESGDFVILRRDKLIAYHLAVVVDDFAQGVSQVVRGVDLLESTPRQVY
                     LQRVLGYPTPEYAHIPVVVNRAGQKLSKTTGSREVPLDRVAPVLCAALRALGQPVSRD
                     LHSAGLSAIWAWSMEHWTLETMINQRSVPEPLEAYG"
     gene            complement(3063..3464)
                     /gene="dksA"
                     /locus_tag="VFY27_10695"
     CDS             complement(3063..3464)
                     /gene="dksA"
                     /locus_tag="VFY27_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX5766025.1"
                     /translation="MRRGEEYMSAEQREHFRSILTAWKRELMNEVDRTVHHMQDEAAN
                     FPDPNDRATQESEFGLELRTRDRERKLLRKIDAALARIDDGTYGYCEETGEEIGLKRL
                     EARPVATLSLEAQERRELAERQFRDRDDRYR"
     gene            3577..>4160
                     /locus_tag="VFY27_10700"
     CDS             3577..>4160
                     /locus_tag="VFY27_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766026.1"
                     /translation="MAAVADFLVLTALLDDFLVPFLLLDLLPAVLFAEGFAAVFLRAA
                     PFPAPADFLLTFFVAFFLTGVLEADFFVDFRADFFVDFFDPAGFFRAADFFFDAFFVE
                     AFFALTLRFLPGAFLLAADFPDAVVFLPPPARFGFLLAAFFAGIFHSCRSEKNAELYI
                     GCPNMEAQFGLFFPGAAGICSRSARLGCLCRSLL"
BASE COUNT          695 a         1267 c         1288 g          910 t
ORIGIN      
        1 ggcgcgtcta cgaaggcaag gtcgcgcgtc tcatggactt cggcgcattc gtgaccattc
       61 tgccgggcaa ggatggcctg gtgcatatct cgcagatttc caacgagcgt gtcgagaaag
      121 tgagcgataa gctggccgaa ggcgatgtcg tcaaggtcaa agtgcttgaa gtcgatcgcc
      181 agggccgcgt gcgtctctcc atgaaggaag tggaagccgc gtagcgttct gcgccgtcac
      241 aggcatgcag gggaccggcg acttgccggt cttttttttg tttcagagtc ctggaatcgc
      301 gaccatctcc gtttcctgct ccgcgagttc accctccaga aatcgggtga tccgcctggg
      361 cagatagtat tgtggtcgcc agggagtgcc gccatcgata aatcccacgt ggccgccttg
      421 ttcactcacc tccagcgtaa cgaagtctga aacctggctc tcatcgggga tgacgtccgg
      481 tgtcatgaag ggatcgtcca gcgcgttgat gatcagggtc gggcgttcga tcgttttcag
      541 gaagcgaatc gagctgcatc gatcgtagta atcatcctta ccggcaaagc cgtgcagcgg
      601 cgccgtcacg gcgtcgtcaa actcggcaaa ggtggcggcg cccatagcct tgccccagtc
      661 aaatgccgcc gtatgcggat tgaacgtccg cgtaacggag agcttcatgc gacgcaacag
      721 gtagttctgg taaactcttg agattccttg gcttagtgcc ttagatgatt cgtgcagatt
      781 caggggcacg ctgacggctg ccgccgcgag gagcggtgta tccgctgcgc tttcaccgag
      841 gtacttgagc agcacgttgc caccgagcga atatccagcc gccagcagtg gcccctcgtg
      901 gccgtcggtc cgcagcttgt ggaggaagta acgaaggtcg ctcgtctcac cggcgtggta
      961 gcggcggggc agccggttgc gataatcacc gcagtcgcga aagtgcagca cagccgctcg
     1021 ccatccgcga ccggcggctg catgcaacag ctgccgggca taggtggact tggctgaact
     1081 ttcaagcccg tgcagaacaa ccaggagggg cgatgtttcg gtggtacggg gtacttcttc
     1141 aagccagtca acaactgtca cgtccccgtc gggcagttcc agcatctccc ggtagagcgg
     1201 cggtcgtggc agcctgagca gcggcaggga cggaaacacg gtctgcatat gacgatttcg
     1261 tagccacgct gccggcctga aatcgctttg gcggattctt cctgcgggtt gtccggtgtt
     1321 cacgggcggt gctcgatttt tctggaagtt ccggctgcat ggtacccggg aacatgggtg
     1381 atggcttgcc gggagccgcg ggcaaaaaaa tggccccctg cttgcgcagg gggccggccg
     1441 tggagtgtta actccttcag gcttctgcaa cagggggcgg gggagcgtat ccctgctgca
     1501 atgcacaaaa taggatattc gccacctgtg tgtcaagcat ctttgaaccg cccctcccgc
     1561 tgcagcgcgg agcctgcttg cgcgacggtt tgacaaactg cccgtagcgc gaggcgacaa
     1621 ccggtagcgc gaatccggct gaggtgtcgc tcgcggggcg gctcctgcag gttcttttcc
     1681 ggagggggtg aaatccggcg atgacccaca gccaacaggc gatctacagg tctagccgac
     1741 cttcttgcgt cccgccgtaa gctgcggttt ccaggtgccc gttgctggcg ctgtaccgga
     1801 acggccggag ttctgcagga acgcggacag gctctgttca agatgcgaag agagcggcaa
     1861 ggctgcccgg ccatcgcagg accggatgat ttgcaggagg aaacgctggc tcaggacagg
     1921 gtcctcttgc tgctcgcgct cgctgatcac ctgcagcagg atgcaccggg tgatgtcgct
     1981 tccgtctttc ttgtcgatga cggcgaagtc gactctctcc agcacgaggt cccggatatc
     2041 cgagagcgtg atgtaccggc tttcctcggt gtcgtaaagg cgacgattgg gatacttgtt
     2101 aatgacgcgc cgggtactca ttcactatct cgggatgcac cgcagctaag atagcggatt
     2161 ttccggctca gccataggct tccagcggct ccggaacgct acgctggttg atcatcgtct
     2221 ccagcgtcca gtgttccatt gaccacgccc atatcgcgga caatccggca gagtggaggt
     2281 cccgggaaac cggctgcccg agggcgcgca atgcggcgca cagcaccggt gcaacccggt
     2341 cgagcggcac ttcgcgcgag cctgtcgttt tgctgagctt ctgtcccgcc cggttcacga
     2401 cgacgggaat gtgcgcgtat tcaggcgtgg gatagcccaa cacacgctgc aggtatacct
     2461 gccgtggagt cgactccagc aagtccacac ctcttacgac ctggctcaca ccttgtgcaa
     2521 aatcatccac caccaccgcc aggtggtacg cgatcagctt gtcgcgccgg agaatgacga
     2581 agtcgccgga ttcggattcc agtcgctgct cttgcgggcc ttgcacaagg tcgacgaaac
     2641 ctaccggctc gttgtctgtc ctgagccgga ttgcgctgtg acgtgagttg cttccggcgc
     2701 ggcaggtgcc gggatagatg cgacccaacg gcccctgagg cgcagtcgcc aggcgctggc
     2761 gcgagcagcc gcacgggtac gcgcgccact cgtcgagcag gcgctcgacc gccgcgcgat
     2821 gctgcgcgtt gttgcggctc tggtagagca ccggcccgtc ccattcgaag ccgtaggtct
     2881 ccagcgcgct gacgatagcg tcagccgcac ctctccgctc tcgtggcgga tcgatgtctt
     2941 cgatgcgcag cagccaacgg cctctgcagg atcttgcctg gagataactt gcggccgccg
     3001 cgaccaggga gccgaaatgc agagggccgg tgggtgacgg ggcaaatcgc cctatgtact
     3061 gcttaccggt aacggtcgtc ccgatcccgg aactggcgtt ccgcaagttc gcggcgttcc
     3121 tgggcttcca gcgacagcgt cgcaaccggg cgagcctcca gccgcttcaa cccgatctcc
     3181 tctccggttt cctcgcaata gccataagtg ccatcgtcga ttctcgcgag agctgcgtcg
     3241 atcttgcgaa gcagtttgcg ttcgcggtcg cgggtgcgca gttcgagtcc gaactccgac
     3301 tcctgggtcg cgcggtcgtt ggggtcgggg aagttcgcgg cctcgtcctg catgtggtgc
     3361 accgtccgat ctacttcgtt catcagctcc cgtttccagg cagtgagaat ggatcggaaa
     3421 tgctcccgct gttccgcgct catgtactcc tcgccgcggc gcatcgaata aggctcgatg
     3481 ccgtgaatcg ggccggccag caggtcctgc cgagacgggc gcttcgcggc gccattcgtc
     3541 cgggcggccg gcgttgcagg cttccgtgca ggctgcgtgg ctgctgtggc cgatttcctg
     3601 gtgctgacag ccttgcttga cgacttcctg gtccccttct tgctgctgga ccttttaccg
     3661 gcggttctct ttgctgaggg ctttgcagca gttttcttgc gtgcggcccc tttcccggca
     3721 cccgccgact ttttgctcac ctttttcgta gccttcttcc tcactggggt cttggaggcc
     3781 gacttcttcg tcgacttccg ggcggatttt ttcgtggact ttttcgaccc tgccggcttt
     3841 ttccgggcgg ccgatttctt cttcgacgcc tttttcgtcg aagctttctt cgcgcttact
     3901 ttacgtttct tacctggtgc ttttttgctg gcagccgact ttccggatgc agtcgtcttc
     3961 ctgccgcctc cagcgcgctt tggtttctta ctcgcggcct tttttgcggg catcttccac
     4021 tcctgccggt ccgagaaaaa cgcggaatta tacatcggtt gcccgaacat ggaagcccaa
     4081 ttcggcctgt tcttccccgg tgccgcaggc atttgcagcc ggtcggcgag gctcggctgc
     4141 ctctgccgaa gcctgctcca
//
LOCUS       DASUOM010000514         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_306223,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000514 DASUOM010000000
VERSION     DASUOM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306223"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFY27_10705"
     CDS             complement(<1..425)
                     /locus_tag="VFY27_10705"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX5766027.1"
                     /translation="MTRPTGKADLPLHGGRVPAWLASRMAALGRVVVEAVVHHYGHEE
                     LLRRLSHPFWFQSFGAVMGMDWHSSGITTSVIGALKRGLEPVQNELGIYVCGGRGRHS
                     RRTPAELTRVGELTGIDGDALAYSSRLVAKVDSAAVQDG"
     gene            complement(425..994)
                     /locus_tag="VFY27_10710"
     CDS             complement(425..994)
                     /locus_tag="VFY27_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX5766028.1"
                     /translation="MNQSELFDPMPLPDGLWYRPDFITPEEEAGLLGAIAALPLQEAH
                     YKEWTAKRRIVSYGGRYDFSRNKLLEAEPVPQFLHPLRERVAQWCEISPTRFSHAMIA
                     EYRPGTQLGWHRDVPNFDCIAGVSLSGPARMRFRPYPPQKGKGRATLALDLQPRSVYM
                     LRGTARWDWQHAISPTKSLRYSITFRTLS"
     gene            complement(1172..1936)
                     /locus_tag="VFY27_10715"
     CDS             complement(1172..1936)
                     /locus_tag="VFY27_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766029.1"
                     /translation="MRSLYFVSTIALGALISGHALGQESTGSNQQSTQQNTTNRQDST
                     GSGQQATANPEPYAKSDDSWISISGTVASPEPDSFSLDYGDGVITVEMDDWDTYGDAY
                     PLMEGDRVTVYGKVDDDLFELSSIEASSVYVEGLGTYFYASSADEEDPVYAEYFWTAP
                     TPLVLNRATLRGTVERIGREDREFTIDTGLQQVTVETDGLEYNPLDDFGFQKVERGDK
                     VSVSGEFDVDFLQGRVFEADSVITLSEANRSNTGSS"
     gene            2401..2772
                     /locus_tag="VFY27_10720"
     CDS             2401..2772
                     /locus_tag="VFY27_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766030.1"
                     /translation="MRNQITLAIAISLLGSPALAQTTFESLDTNSDGSISKDEYYGLV
                     SDAGIYPDLDSDSDGFIEENEFEGIGLDDDYASWDLNRDDYVDSREFYDGYFGYYDAN
                     EDAHWDGDEWDDAGEAGILDF"
     gene            complement(2844..3980)
                     /locus_tag="VFY27_10725"
     CDS             complement(2844..3980)
                     /locus_tag="VFY27_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766031.1"
                     /translation="MTVLTVVACSDNHVVAIAPDPEPPSETFDPQDTFRYDTYGDEAL
                     WTDTLRFHDVVATISPETALAVGLKVDAEVLPDGLLASADLTDPATTIELLRLDAVVG
                     VKATVTEDGRVTSFGITCALCHSDVDDSVAPGIGIRQDGWPAKDLDPGLILSLSPFFD
                     DKPQQREELRSWGSGKYDPYWNLDGINDPVVIAPAYGLKDVPLETYTGEGQVSYWNAY
                     VAITQMGGQGNFEEPLLNISVQADPDLVTPKLPALLEYQESLEPPLPPAGSYDETAAE
                     RGKVLFEGKAECAGCHFGPRLTDAAVTLHEAHEVGADPLYATRTTTRMYRTTPLRALW
                     QHPPYYHNGEFATLKEVVDHYDLVLNLGLFDAEKSDLVEYLKSL"
     gene            complement(4254..4409)
                     /locus_tag="VFY27_10730"
     CDS             complement(4254..4409)
                     /locus_tag="VFY27_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766032.1"
                     /translation="MADGFWVVALLIAAVLVYVVAQVIFYSRKSRQQWREVDRSKLRK
                     WEDEDKW"
BASE COUNT         1015 a         1420 c         1349 g         1019 t
ORIGIN      
        1 tagccatcct gcacggctgc gctgtccacc ttggctacaa gccgactgga ataagcgagt
       61 gcatcgccat cgatgccggt caattcccca acacgcgtaa gttccgcggg tgtccggcgc
      121 gagtgccggc cgcgaccacc gcagacgtag atgcccaatt cgttctgcac cggctccaat
      181 ccccgcttga gagcgccgat gacgctggtg gtgatccccg aagaatgcca atccatgccc
      241 atgacggcgc cgaaggactg gaaccagaac ggatgcgaca agcgtcgcag cagttcctcg
      301 tggccgtagt gatgtacaac cgcttctact acaacgcggc cgagcgcggc catgcgactc
      361 gccagccacg cgggcaccct cccgccatgg agcggtaaat cggctttgcc ggtaggtctg
      421 gtcactacga cagggttcgg aaggtgatgg aatagcgcag gcttttggtt ggcgaaatcg
      481 cgtgctgcca atcccaccgc gctgttcctc tgagcatgta aacagatcga ggctgcaagt
      541 cgagcgccag tgtcgcacga cctttaccct tttgtggtgg ataaggacgg aatcgcatgc
      601 gtgccggacc tgatagggac actcctgcga tacagtcaaa gttcggcaca tcacgatgcc
      661 agccaagctg cgtacccggc cgatattcag caatcatggc gtggctgaac cgggtgggtg
      721 agatctcgca ccattgagcg acgcgttcgc gcaatggatg caaaaactgc ggcactggtt
      781 cagcctccag cagtttattc cggctgaaat cgtaacgtcc gccgtaactg acgatacgac
      841 gcttcgcggt ccactccttg taatgcgctt cctgcaacgg cagagccgca atggctccca
      901 gcagccctgc ctcctcctcc ggcgtaatga aatcaggccg gtaccacaag ccgtccggga
      961 gtggcatagg gtcgaacagt tcactctggt tcaagacact ggaaattgag gtgaggtcag
     1021 ggctgtgaat atatacagct atcggtcaga attccagtca ggaaacttct cctacctctc
     1081 catcggtcca ccgaaaggag gagccggaac caggcttgaa ccacggggcc ggccctcctt
     1141 ccgaacgatc caagaccaga cgaaactggc gttacgacga gccggtatta ctccggttcg
     1201 cctcagaaag cgtgatcacg ctgtcggcct cgaagacgcg cccttgcaga aagtcaacgt
     1261 caaattcgcc ggacacgctg actttgtcgc ctctttcaac cttctggaag ccgaaatcat
     1321 cgagcggatt gtactcgagt ccgtctgtct cgaccgtcac ctgttgcaaa ccggtatcga
     1381 tagtgaattc gcgatcttct cgaccgattc gctccacagt cccgcgaagt gtcgcgcgat
     1441 tcaaaaccag cggcgtcgga gccgtccaga agtactctgc gtataccggg tcttcctcgt
     1501 cggcggaact tgcataaaag taagttccga ggccttctac ataaacgctg cttgcctcga
     1561 tactggaaag ttcgaaaaga tcatcgtcca ccttgccgta aacagtgacg cggtccccct
     1621 ccatgagggg atatgcatcg ccgtatgtat cccagtcatc catctcgacg gtgatcacgc
     1681 cgtcaccata gtcaagtgag aacgagtcgg gctcgggcga tgcgaccgtg ccgctgatgc
     1741 tgatccagct atcgtccgat ttggcgtacg gctcagggtt ggcggtagcc tgctggccgg
     1801 agccggtact atcttgtctg ttggtagtgt tctgctgagt gctttgttga ttggaaccgg
     1861 tactctcttg ccccagcgcg tggccggata tcaatgctcc cagagcaata gtgctgacaa
     1921 agtaaaggct tcgcatcagt tgcctccttg gtagtagttg ctggattttt cgcgaatctc
     1981 ggggtgcaag tgatgtgcca ataggcaatg cagttaggta tgaggaggca ttgggagaat
     2041 cccggcctcg taacaactga tgcaggatct atgacatgta atatctgcac ttcaacccag
     2101 aggcgtgggc atcacgccgc atcactgaca cataccataa gtcttcactg tttccagatt
     2161 ttgcgttagg tcgtagattt tacgcacagc cgcccgatga tttaatttac ggggtgcagc
     2221 agctcgcggc gatatcaagt tcagccctcc gaggccaaca atggtcccgc tgcccttcac
     2281 cgcagctttg ccgcattatc gaaagcgtcg acctcccgct ggcacactgt ttgcatttat
     2341 ctctcctcca aatttgctgc tgtgcagctt tgatcaactt caatgacagg aggaacaaac
     2401 atgcgcaacc agatcacact cgctattgca atctctctgc tgggttcacc ggcacttgca
     2461 caaacgacct ttgaaagtct cgacacgaac agcgatggca gcatttcaaa agatgagtat
     2521 tacggcctcg ttagtgacgc cggcatctac ccggacctgg actcggactc ggatgggttc
     2581 attgaggaga atgagttcga aggaatcgga ctcgacgacg attatgcttc ctgggacctg
     2641 aatagagacg attacgtgga ctcccgggaa ttctacgacg gctattttgg gtattacgac
     2701 gcgaatgagg atgctcattg ggacggcgac gagtgggatg acgctggcga agccgggata
     2761 ctggacttct aaagtggcgt gagtgcccgc tggctggtat ctaggtcggc accccgctat
     2821 tgcaggcgta tcctccggga ttgtcataga gacttgaggt actcgacaag atcgcttttc
     2881 tccgcatcga acaggcccag gttcaacacc aggtcatagt gatcaacaac ctccttaagc
     2941 gtcgcaaatt cgccgttgtg ataataagga ggatgctgcc acaatgcccg gagcggcgtg
     3001 gtgcgataca tccgcgtggt ggtgcgcgtc gcgtacagcg ggtcggctcc cacttcgtgc
     3061 gcctcatgca aggtaacggc cgcatccgtt aggcggggtc cgaagtggca gccagcgcac
     3121 tcggccttgc cctcgaacag caccttgccg cgttcggcag ccgtctcgtc atagctgccg
     3181 gcaggcggca gtggcggttc gaggctttcc tggtactcga gcaacgccgg aagtttcggc
     3241 gtaacgagat ccggatccgc ttgcacggag atgttcaaca acggctcctc gaagttgccc
     3301 tggccgccca tctgcgtaat cgccacgtat gcgttccagt aggaaacctg cccctcaccc
     3361 gtgtaggttt ccagcggaac gtctttcagg ccgtatgccg gggcaatgac caccggatca
     3421 ttgatgccgt cgaggttcca gtacggatca tatttacccg aaccccagga cctgagttct
     3481 tcgcgctgtt gcggcttgtc atcgaagaaa ggcgacaagg acagtatcag gcccgggtcc
     3541 aggtccttgg caggccagcc gtcctggcgg atgccgatgc cgggagcgac ggaatcatcc
     3601 acgtccgaat ggcacaacgc gcaggtgata ccgaacgatg tgactcgacc atcttcggtc
     3661 accgtcgcct tcactcctac cacggcatcc agtcgaagca gctcgatggt ggtcgccgga
     3721 tcggtcaggt ctgcacttgc gagtaaaccg tccggcagta cttccgcatc caccttaagc
     3781 ccgacagcca gtgctgtttc agggctgatc gtcgcaacta cgtcatgaaa ccgcagggtg
     3841 tcggtccaca aagcctcatc accataggtg tcatagcgga aggtgtcctg cggatcaaag
     3901 gtttccgatg gcggctccgg gtccggtgcg atcgcgacaa cgtggttgtc cgagcacgcc
     3961 accaccgtca ggacagtcat cagcaagacc gtttccaggc aattccgcaa ccggaccatg
     4021 tccttcctcc cgtaatgggt cgtagtcgca cacaggatgc gggccctgcc gaaaaggttc
     4081 ccgtgtagcc gcctgaacga acagcgacgg cgtgcgtcac ggaggaactg cctggttgct
     4141 tcgatttctt tggatgtgtc cggacgcgcc ggcgcggcga gcaacttatc ccgcgaatgg
     4201 gtggagtgcg ttggcgttga tcgctgggtg aggcgacctt taggcttcgc cgttcaccat
     4261 ttgtcttcgt cttcccactt ccgcagtttg ctgcggtcga cttcgcgcca ttgctgacgg
     4321 cttttcctgg agtaaaaaat cacctgcgca acgacataga cgagaaccgc agcaatcaag
     4381 agggccacaa cccaaaaccc gtcagccatc ccggaaccct cgcatcgatc cctggtgcag
     4441 ccactcgcgg catcgcgccc tgaaccagga gtcacattgg caatgtggga tattcaggat
     4501 aacgcaactc gttcacggac gcgcagcccg tcgattcctg cgactttccc ggcttaaacg
     4561 tgaaggtcag acttgcgacc agccggggct agcacctgcc tggccggaac tacgctaccg
     4621 caggacttgt ccaacgcctc cttaaaggga gctcgggatg atcggtcggt cgggggaggt
     4681 cgagcgtaga tgccatgtcg ccacacgcga tgttattgcg gtggcgctta ccttcatgct
     4741 tcacctcggg ggctgcggcg gctctgctac acccccatcc gcgactgaac cgccaacacc
     4801 gcc
//
LOCUS       DASUOM010000515         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1753188,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000515 DASUOM010000000
VERSION     DASUOM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753188"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VFY27_10735"
     CDS             complement(<1..1728)
                     /locus_tag="VFY27_10735"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX5766033.1"
                     /translation="MRPNLLAGVLALLLALPALSEEETATLPTGVEIDLGPAGAVTLR
                     RYEHDAVAVEVDGSLDESVWSELPVASDFRVLEPDTLAVPPYRTALRFFYTEHGLYLG
                     FDLEQPPDTIVRRYVPRDNLDIKRDNVSFTLDTSGNGRYGYWMNVSLGDSQRDGTILP
                     ERQYSTEWDGAWYGASAGTARGWSAEVFIPWSQVAMPKEPGLRRMAFYTQREVAHRNE
                     IWGFPALPESQSQFMSSFQPLILEGIDPRQQWSVFPYASSSFDRVDDDLRYKAGLDLF
                     WRPTSNFQLTATANPDFGSVESDDVVVNLTANETFFPEKRLFFQEGQEIFQATPRSEI
                     EGDQRFSVVNTRRIGGRPDDPDLPPGTSLPARDALAVADLLGAAKATGQYGGFRYGVL
                     AAFEDETAFRIDDAEYLVDGREFGTVRLLYEDSRGAAYRGLGFLSTAVLRPDAEAFVH
                     SADLHYLSRGGRWKFDGQLVHSERDDLGGGNGATADFTFTPRQGLKHQLQMAWFDERL
                     NVNDLGFQIRNDNRDVWYRMEWVRSGLSRVRDFKLTPFLRYEENGDGYRTNNAVASGY
                     EITLNNLHTL"
     gene            complement(1885..2319)
                     /locus_tag="VFY27_10740"
     CDS             complement(1885..2319)
                     /locus_tag="VFY27_10740"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5766034.1"
                     /translation="MGEGNTYDGGCFCGAVRLSVTGEPVGMGYCHCESCRRWSAGPVN
                     AFTLWEPGSVRIKAGEEKLGSYSRTPASIRKWCTTCGGHVLTEHPGMGVTDVYAAVIP
                     DFPYRAGVHVHYQETKLPMRDGLPKMKDVPAEMGGSGSTVAE"
     regulatory      2568..2655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          502 a          837 c          848 g          511 t
ORIGIN      
        1 taacgtgtgc aggttgttca gcgtgatctc gtaaccggat gcgacggcat tgttggtgcg
       61 gtagccgtcg ccgttctcct cgtaacgcag aaacggcgtc agcttgaagt cgcgcacgcg
      121 gctcaagccc gacctcaccc actccatccg gtaccagacg tcgcggttgt cgttgcgaat
      181 ctggaagccc aggtcgttga cgtttaagcg ctcgtcgaac caggccatct gcaactggtg
      241 cttcaggccc tggcgcggcg taaacgtgaa atccgctgtc gcgccgttgc cgccgccgag
      301 atcgtcgcgt tccgagtgca cgagctggcc atcgaatttc caacgtccgc cgcgcgacag
      361 gtagtgcagg tccgccgagt gaacgaatgc ttcggcgtcc gggcgcagga ctgctgtcga
      421 caggaaacca aggccgcggt acgccgcgcc gcggctgtct tcgtagagca ggcggactgt
      481 gccgaactcg cgcccgtcga ccaggtactc ggcgtcatct atccggaagg ccgtttcatc
      541 ctcgaacgcc gccagcacgc cgtaacggaa accgccgtat tgcccggtcg cttttgccgc
      601 tccgagcaga tcggcgaccg cgagcgcgtc gcgagcaggg agcgacgtgc ccggcggcag
      661 gtcaggatcg tcgggcctgc cgccgatcct ccgcgtgttg acgacggaga agcgctggtc
      721 gccctcgatc tcgctgcgcg gcgttgcctg gaagatctcc tgcccttcct ggaagaacag
      781 ccgcttctcc gggaagaagg tctcatttgc cgtgagattg accaccacgt cgtccgattc
      841 caccgaaccg aagtcggggt tggcggtggc cgtcagctgg aaattgctgg tcggccgcca
      901 gaacaggtcc aggccggcct tgtaacggag gtcatcatcg accctgtcga aggacgatga
      961 cgcgtaggga aatacgctcc attgctgccg cggatcaatc ccttccagta tgagcggctg
     1021 aaatgaactc atgaattgcg actgggattc gggcaaggcc gggaatcccc agatctcgtt
     1081 ccggtgggca acttcgcgct gcgtataaaa ggccatgcgc cgcaggcccg gttccttggg
     1141 catggccacc tgcgaccacg gtatgaatac ctcggcactc cagcctcgcg ccgtcccggc
     1201 cgaggcgccg taccaggcgc cgtcccactc ggtactgtat tgacgttcgg gcagtatggt
     1261 gccgtcgcgc tggctgtcgc cgagtgagac gttcatccag tagccgtagc ggccgttgcc
     1321 ggaggtatcg agcgtgaagg acacgttgtc gcgcttgatg tccaggttgt cacgcggcac
     1381 gtaacgccgc acgatcgtgt cgggcggctg ttcaagatcg aaaccgaggt acagcccgtg
     1441 ctccgtataa aagaaacgaa gcgccgtgcg gtatggcgga acggccaggg tgtctggttc
     1501 cagcacgcga aaatcgctgg ccaccgggag ctctgaccag acggattcgt cgaggctgcc
     1561 gtcgacttcg acggcgactg cgtcatgctc gtagcggcgc aaggtcaccg ctccggcagg
     1621 tccgaggtcg atctcgactc cggtgggaag cgttgcggtc tcttcctccg acagcgcggg
     1681 tagcgcaagt aacagcgcga gtacacccgc caacaggttc ggtcgcaagg ctcccccctt
     1741 gaaaagggca agtctacatg gccggaggtc gccggtttgg atatacgaac gcaatgcgga
     1801 cgatacgggc gcgggtgcat ctacccctgc tactggacgt gcatagcggc cccggctgct
     1861 ggagtgggtg gccggccgag gctgctactc ggcgactgta gagccgctgc cgcccatctc
     1921 agccgggacg tccttcatct tcggcaggcc gtctcgcatc ggaagcttcg tttcctggta
     1981 gtgaacgtgc acgcccgcac ggtagggaaa gtcggggatg accgccgcgt aaacgtcggt
     2041 aacgcccatc ccgggatgct cggtcaacac gtgtccacca caggtggtgc accattttcg
     2101 gatgctcgct ggcgtcctgg agtaagagcc gagcttctcc tctcccgctt taatgcgcac
     2161 ggacccaggc tcccataggg taaaggcatt gaccggcccc gccgaccagc gacggcagga
     2221 ttcgcaatgg cagtaaccca ttccgacggg ttcgccagtc accgacagcc tgaccgcgcc
     2281 gcagaaacac ccaccgtcgt aagtattgcc ctcgcccata tcccgtctcc gtaaagccgc
     2341 ggcggaaatc gctgcggtgc atgcattctg ccgaaaagtt tgcggactat cgatagctca
     2401 tcagcccgct ttggagcgga cttcgggtag ctccttcttc agcggccgtt atgtaaagaa
     2461 atgtgacgca gtgcacattt tcttcagaat cgtgaccggg gtctcagttt cgcggaccgc
     2521 ggaggaacta tgttgagcct gcagtcgatg gacggctgca ttggggggtc cgataacggc
     2581 aaacgcatcg aaaggtgcgg acgcaaagct ccgggtctaa agccctcgac gggccatgac
     2641 ggccgagtta ccgaagacat tccggtttta tccagtggag cggatgcaat ccgtcttc
//
LOCUS       DASUOM010000516         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_306903,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000516 DASUOM010000000
VERSION     DASUOM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306903"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..886
                     /locus_tag="VFY27_10745"
     CDS             131..886
                     /locus_tag="VFY27_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HEX5766035.1"
                     /translation="MNCEEYKEAIATDPSASFAGDDAHASGCDACSKYRDEMRALDER
                     IARALAIDVPELRLPDLPYIEPAGGEKSAAAANPGAPGIRSPFMSPPLWVGLAAAIAL
                     VAILFTGLPETRTDHRQLVAEIVAHMDHEQASRQVTSVPVPAQTLHAVVDPKVAAMDR
                     DVGLITYAMSCVINGRTVPHLVIQGSTGPVTLILLPDETIESPLSLVGQNVQGVLLPV
                     GHGSIAVIGQREDQMDEIDRIGKELADSVEWSI"
     gene            991..1365
                     /locus_tag="VFY27_10750"
     CDS             991..1365
                     /locus_tag="VFY27_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766036.1"
                     /translation="MKAKKAARPVALAVGAALAGSFAMSGVASADTGASPFAMTTLNA
                     GYMLGAGEGACGGDKAKAEGNCGGDKGAEGNCGGDKEDKGAEGNCGGDKEDKDAEGNC
                     GGDKEDKDAEGKCGEGKCGGAV"
     gene            1528..2154
                     /locus_tag="VFY27_10755"
     CDS             1528..2154
                     /locus_tag="VFY27_10755"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5766037.1"
                     /translation="MRDDMVQQKTKARQKRCDWAGDSSLQYIEYHDKEWGVPVWDDRT
                     QFEFLILEGAQAGLSWSTILRKRDGYRKAFADFDPQKVARFTQKRIDKILQNPGVVRN
                     KLKVNAAVTNARAFLEVQEEFGTFCDYVWRFVDGQPKQNRLKKQADIPATSPDSDAMS
                     KDMKKRGFRFVGSTILYAHMQATGMVNDHLVSCFRYKQCAAIGRKKAA"
     gene            complement(2167..3096)
                     /locus_tag="VFY27_10760"
     CDS             complement(2167..3096)
                     /locus_tag="VFY27_10760"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5766038.1"
                     /translation="MPAPERFSGVLAPVITPFLEDLAPDPRRLAAHCRWLSSQGAGLA
                     LFGTNSEGNSLSVGEKMELLDRLIEAGIDPARMMPGTGCCALSDSVELTRHAVRGGCG
                     GVLMLPPFYYKGVSDEGLFRSYSEVIERIGSSDLRIYLYHIPPVAHLGISIELVERLI
                     TAWPETIVGIKDSSGDWDNTNKLLELAADDFRVFSGSESFLLRNLRAGGAGCISATAN
                     VNPGAIRRLCEEWRSPAADALQRDLNELRDCLQLPWMIPGLKAVVAYFLDDPGWETVR
                     PPLIPLEPARKSALIAKLSQRRFAMPGLAPGVG"
     gene            complement(3150..>3783)
                     /locus_tag="VFY27_10765"
     CDS             complement(3150..>3783)
                     /locus_tag="VFY27_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766039.1"
                     /translation="EVQATGAAGTFAFPVEFGYTGSYSPGVHGLRAPLVIQGFVDEDP
                     NKTFTFRTENGVTAHLIDVPSEQAYLRFALYDELTDGDDDLDMYVYYCPDSVNCTKIG
                     ESGEPTSREQFNVLLPGAGRYAVLIHGFDTDNTAGGPGANYSLLGWAFGLSDDQGNMT
                     ASGPGFVNAGTTDAVQLSWSGLAADTIYLGGISHNTPQGLVAITVVTIQN"
BASE COUNT          760 a         1119 c         1232 g          672 t
ORIGIN      
        1 gcacgaaaga aattgccgaa ctcatgggga tccggcaagg agccgtcctt acccgcctcc
       61 accgcgctcg cctgaagctg aaagattcca tcgatgcgac gggatgccgc acggtgatca
      121 gggacagcgg atgaactgcg aggagtacaa ggaagccatc gcgacggatc cgtcggcatc
      181 gtttgccggc gacgacgctc atgcgtccgg ctgcgatgcc tgcagcaagt accgtgacga
      241 gatgcgcgcg ctcgatgagc gcattgcacg cgccctggcg atcgatgtgc ctgagttaag
      301 gctgccggac ctgccgtaca ttgagccggc cggcggtgag aagtctgctg ccgcagccaa
      361 tccaggcgcc ccgggaatcc gatctccctt catgtcgccg ccgctctggg tggggctggc
      421 tgctgcaatc gcgcttgttg caattctttt cacaggactg cccgaaacgc gcacggatca
      481 ccggcaactg gtcgcagaga tcgtcgcgca catggatcac gagcaggcgt cacgtcaggt
      541 cacttcggtc ccggtgccgg cgcagaccct gcatgcggtt gtcgacccga aagtcgcggc
      601 gatggatcgc gacgtaggtc tcatcactta cgcgatgagt tgcgtgatca acgggcgcac
      661 ggttccgcat ctcgtcatcc agggcagcac gggacccgtc accctgatcc tgctgcccga
      721 cgaaacaatt gaatcgccgt tatctcttgt tgggcagaat gttcagggcg ttctcctgcc
      781 ggtcggtcac gggagtattg cggtcattgg acagcgcgaa gaccagatgg atgaaatcga
      841 caggatcggc aaagagctcg ccgattccgt ggagtggagt atctgaacat cgggggaagg
      901 tagggtgctt ctggaaggcc gtcccttttt ccgtcaccgg cgccggctcc tgcgacgcct
      961 ttagcgaaac tggagagagg agatttgaag atgaaagcca agaaagcagc aagacccgta
     1021 gccctggccg tgggcgcggc actcgccggc tcattcgcca tgtcaggcgt tgcgagcgcg
     1081 gatacgggcg caagcccgtt tgcgatgact accctgaacg ctggttacat gctgggcgcg
     1141 ggtgaaggcg cctgcggcgg cgacaaggcg aaggcagaag gaaactgcgg cggagacaag
     1201 ggtgccgaag gcaattgcgg gggcgacaag gaagacaagg gtgccgaagg caattgcggc
     1261 ggcgacaagg aagacaagga tgccgaaggt aattgcggcg gcgacaagga agacaaggac
     1321 gccgagggca agtgcggcga aggcaaatgc ggaggcgctg tttagccggg cgagagaaaa
     1381 cggaagctcc ctggacggtc cagggagctc ttctgcctcg atcgcaacac gcgccgtcga
     1441 tgcttttact tcgcgttaca tgaacttttg ttcgctgcag gccgggtgtg agattgctat
     1501 tcttcgctca ggatctggat actcggggtg cgggacgaca tggtgcagca gaaaaccaag
     1561 gccaggcaga aacgttgcga ctgggccggc gacagcagcc tgcagtacat cgagtaccat
     1621 gacaaggagt ggggtgtgcc ggtgtgggac gaccggaccc agttcgagtt cctgatcctg
     1681 gaaggcgccc aggcgggact cagctggtcg acgatcctta gaaagcgcga tggttacagg
     1741 aaggccttcg ccgacttcga tccgcaaaag gtcgctcgct tcacgcagaa gcggatcgac
     1801 aaaatcctgc agaatcccgg agtcgtgcgt aacaagctaa aggtcaacgc ggctgtgacc
     1861 aatgcccggg cattcctcga ggtccaggaa gagttcggca cgttctgcga ctacgtctgg
     1921 cgatttgtcg acggccagcc gaagcaaaac cggctgaaga agcaagcgga cattcccgct
     1981 acatcgcccg actccgatgc gatgagcaag gatatgaaga agcgtggctt ccgattcgtc
     2041 ggaagcacca ttctttacgc gcacatgcag gcgaccggca tggttaacga ccacttggtc
     2101 tcctgctttc gctacaagca gtgcgcggcg ataggccgaa agaaagctgc ctgaccgcgt
     2161 tgccgcttac cctacgccgg gtgcaagtcc aggcatggcg aaccggcgct gcgacagctt
     2221 cgcgatcaac gcggacttcc ttgcaggctc gagcgggatc agcggtggcc gcacggtttc
     2281 ccatcccggg tcatcgagga aatatgccac caccgctttc agcccgggga tcatccacgg
     2341 cagctgcagg caatccctca gttcattgag gtcccgctgc aatgcgtcgg ccgcggggct
     2401 ccgccactcc tcgcaaagcc tgcggatggc gccagggttc acgttcgccg tcgccgaaat
     2461 gcatcccgct ccgcctgccc tcaagttgcg caacaggaag ctttcactgc cggagaacac
     2521 ccggaagtcg tcggcggcta actcgagaag cttgttcgtg ttgtcccagt cgcctgagct
     2581 gtccttgatc ccgacaatgg tttccggcca cgcagtgata agcctttcca caagctcgat
     2641 cgagatgccg aggtgggcga caggcggaat gtgataaagg tagatgcgca gatcgctgct
     2701 gccgatgcgc tcgatgactt ccgagtaact gcggaacagc ccctcgtcgc ttacgccttt
     2761 gtagtaaaag ggcggcagca tcagcacgcc gccgcaaccg cctctcacgg catgtctcgt
     2821 cagttcgaca gagtcgctca aggcgcagca gccggtaccg ggcatcatgc gcgccgggtc
     2881 gatgcccgct tcgatgagcc ggtcgagcag ctccatcttt tcgcccacgg aaagcgagtt
     2941 cccttcgctg ttggtgccga agagggcaag ccccgcgccc tgggacgaaa gccatcggca
     3001 gtgcgcggcc agccgcctcg ggtccggcgc gagatcttcg aggaacgggg tgatgacggg
     3061 ggcgaggacg ccggagaatc tctcgggtgc gggcatcgca ggacgttcct tatcagcagg
     3121 gaaggacggc gcagggcgta cgccgccgtc tagttctgga tcgtaacgac ggtgatggcg
     3181 accaggccct gcggggtatt gtgcgaaatc ccgcccaggt aaatcgtgtc tgccgccagc
     3241 cccgaccagc ttaactgaac cgcgtcggtc gtgccggcgt tgacgaaacc cgggccgctc
     3301 gcggtcatgt ttccctggtc gtcggacagg ccgaacgccc agccgagcag cgaatagttc
     3361 gcaccgggac cgcccgccgt gttgtcggtg tcgaagccat gaatcaggac cgcatagcgg
     3421 ccggcgcccg gcaacaacac gttgaactgt tcacgggagg tcggttcgcc gctctcgccg
     3481 atcttcgtgc aattgacgct gtccgggcag tagtagacgt acatgtccag gtcgtcgtcg
     3541 ccgtccgtca gttcgtcgta gagcgcgaaa cgcaggtagg cctgctccga cggaacgtca
     3601 atcaggtgcg ccgtcacgcc gttttcggtg cggaaggtga acgtcttgtt cgggtcctcg
     3661 tcgacgaagc cctggatgac gagtggggcg cgcaggccgt ggacgccggg cgagtagctg
     3721 ccggtgtaac cgaactccac cgggaaggcg aaggttccgg cggcgcccgt cgcctgtact
     3781 tcg
//
LOCUS       DASUOM010000517         5703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_577232,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000517 DASUOM010000000
VERSION     DASUOM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577232"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFY27_10770"
     CDS             complement(<1..842)
                     /locus_tag="VFY27_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5766040.1"
                     /translation="MPVSGKLFIGARQVTTGHSFRAVNPASGDVLEPPFSAAGLPGVE
                     QACELAWSAFHEYRRLDPGIRATFLETIADEIMALGNELLERGTAESGLPIARLTGER
                     ARTAGQLRLFADELRTGGWLGIRKDPALPDRKPLPRPDLKLRKVPLGPVAVFGASNFP
                     LAFSVAGGDTAAALAAGCPVIVKGHSAHPGTSDLVAQAVIEAVRRCVLPDGVFSLLNG
                     DSHALGGALVAHPHIKAVGFTGSRRGGLALMKIAGERPEPIPVFAEMSSINPVLLLPG
                     ALAA"
     gene            953..2668
                     /locus_tag="VFY27_10775"
     CDS             953..2668
                     /locus_tag="VFY27_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX5766041.1"
                     /translation="METSLITTLDWYVIGLYFAIIGFIAWWYGRDNKDSVDYFLAGRN
                     AGFIAIGASIFTSNIGSEHIVGLAGQGASTGMAMAHWELHAWILILLAGFFVPFYYKA
                     GVQTIPEFLERRFNSRARTILSVVSLVGYVFTKVSVTVYAGAIVFQTLLPDTFGTPDN
                     AFWVGAFTTVILTGLYTVFGGMRAIMATATPQAVIILFGAFTITWLGLTTLGEGAGVI
                     AGWGELVSTVQVNKEQFALWRPLSDPNFPWLGVMIASPIVGIWYWCTDQYIVQRALSA
                     KNLTTARRGALFGGLLKVWPVLIFLVPGLIGWSLHEKGLIELPMRMVNGVSEINGDTV
                     FPALVSTLLPSGIRGLIVACLLAALMSSLASLFNSSASLFTVDVYEKLRPGQTEKHLL
                     LVGRIATTVVVGLGMLWIPVMKVISGGGLYQYLQSVQGYLAPPITAVFLLGLFWTRLN
                     GRGAVWALVGGFVLGMGKLTCQALFGEGKIESPAILAAIGDFNFLYATGVLFVASAAL
                     MIIGSLTSAPPPAEKIDGLTYRSIRVMHGDEIRASWDAGNKFMATAILVLVAGIYLYF
                     SFWLN"
     gene            complement(2675..3370)
                     /locus_tag="VFY27_10780"
     CDS             complement(2675..3370)
                     /locus_tag="VFY27_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5766042.1"
                     /translation="MHGSIARDLGVLIVSGRYKPGELLNGEIAASDRLQVSRGAYREA
                     VRILAAKGLVESRPKVGTRVSPPGRWHLLDPDVLSWIFEFEPDEGLLENLFELRKIVE
                     PEAAALAALRRTDEDLDAMARALDGMATHTLAVEAGRLADQEFHSALLRASGNAFLVS
                     LTSGIAAAISWTTVFKQRNNPLSRDPVPDHKSVYDAVKSADPEAAHAAMANLVDLAFL
                     DTTNSRRIRKKGT"
     gene            complement(3711..3899)
                     /locus_tag="VFY27_10785"
     CDS             complement(3711..3899)
                     /locus_tag="VFY27_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766043.1"
                     /translation="MTEERKGGEGNREADRRYRKGVKETVEKTSEDERSDKARDVSRE
                     ELKKARQAEEKGKSRARD"
     gene            complement(3989..4666)
                     /locus_tag="VFY27_10790"
     CDS             complement(3989..4666)
                     /locus_tag="VFY27_10790"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5766044.1"
                     /translation="MTNTAVRPAPSSSSDESLLAEVLSGSLESFEGIMRHHNQRLYRL
                     ARSIVTDDSEAMDVVQEAFITAYRRLDDLKEPAALGTWLARITRNAALMRLRGNRRFQ
                     TMEESDLDNVLEMSVTTPRQLLPEREVANMQLRSLLEECIDELPDAFRAVFVLRAVEQ
                     CSVDDTADILEIPQATVKTRFHRARRLMRQRILDYTEAAGIGVHEIAGPRCDAIVNKV
                     MATLRTI"
     gene            complement(4663..5085)
                     /locus_tag="VFY27_10795"
     CDS             complement(4663..5085)
                     /locus_tag="VFY27_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5766045.1"
                     /translation="MKIAAGTAVAAATCLGFFTLLVAAAPAAGDAAAGRRSYAKCTGC
                     HSPDRNRTGPRHCGLFGRTSGTLPGYDYSEAMRKAAILWDAGTLDRFLAAPMTVVPGT
                     TMGFAGVADARERRNLVAYLGSLTASAEECSDTDGELK"
     gene            complement(5101..>5703)
                     /locus_tag="VFY27_10800"
     CDS             complement(5101..>5703)
                     /locus_tag="VFY27_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5766046.1"
                     /translation="MKTIRQASDFQLLAETAKDSRRRFLQQAGTATFSGLAIALLGGR
                     STALAAGTADDAVSQDVRILNTPIAPEHEAVAPYQLGADSGLLQPEVAKVALAFQDHH
                     KQHVEALAKAVRGLGGKDAHALQKYDFPVSKLKAQSDVLQFAAGLERGAVSAYAGAIP
                     LFGNRDLSSAAASILADEAMHWAVLRNALGLDPVPGAFFS"
BASE COUNT          973 a         1775 c         1790 g         1165 t
ORIGIN      
        1 tttgctgcca gcgctccggg aagcagcaac accgggttga tgctgctcat ttcggcaaac
       61 acgggaatcg gttccgggcg ttcgccggca atcttcatga gggcgagacc gccgcggcgc
      121 gaaccggtga agccgactgc ctttatatgc gggtgagcta cgagcgcccc cccaagcgcg
      181 tgcgaatcgc cgttcagcag cgagaacacc ccgtccggca gtacgcaccg gcgcaccgct
      241 tcgatgacag cctgcgccac gagatcgctg gtgccgggat gcgccgagtg gcctttcacg
      301 ataacggggc atccggccgc gagagccgcc gccgtgtcac cgcccgccac ggagaaagcc
      361 agcgggaaat tgctggctcc gaagacggcc acggggccca gcgggacctt gcgcagttta
      421 agatccggcc gcggcagcgg cttcctgtcc ggcaacgccg ggtctttgcg gataccgagc
      481 cagccacccg ttcgcaactc gtcggcgaac agtcgcagct gccctgccgt ccgtgcccgc
      541 tctccggtca gccgggcgat cggcagaccg gattcggcgg tgccgcgctc cagcagttca
      601 ttaccgagcg ccatgatttc atctgcaatc gtttcgagaa atgtggcacg gatcccgggg
      661 tcgaggcggc ggtattcgtg aaacgccgac cacgcgagtt cacacgcctg ctcgacaccg
      721 ggcaggccgg cagcactgaa cgggggctcg agcacatctc ccgacgccgg gttcacggcg
      781 cggaagctgt gaccggtcgt cacttgccgg gcgccgatga aaagttttcc ggaaacgggc
      841 atgggctctc cgttactatg cgctgcactg tgttccatga ggcagcacat cggtttggac
      901 aagcagtaat aactcagaca ttattacacc aatcgagggg gagattagca acgtggaaac
      961 ttctttaata acaacactcg actggtacgt catcggcctc tatttcgcga tcatcggctt
     1021 catcgcctgg tggtacggcc gcgacaacaa ggacagcgtc gactactttc tcgccggccg
     1081 gaacgccggt ttcatcgcca tcggcgcctc gatattcacg tccaacatcg gctccgagca
     1141 catcgtcgga ctcgccggac agggcgcatc gacgggcatg gcgatggctc actgggaact
     1201 gcatgcctgg atcctgatcc tgctcgccgg cttctttgtc ccgttctatt acaaagcggg
     1261 cgtccagacg attccggaat tcctcgagcg ccgcttcaat tcccgcgcgc gcaccattct
     1321 ctcggtggtg tcgcttgtcg gctacgtgtt caccaaggtg agcgtcaccg tctacgccgg
     1381 cgccatcgtg ttccagacgt tgctgccgga cactttcggt acgcccgaca acgcattctg
     1441 ggttggcgca ttcaccaccg tcattcttac cggcctctac accgtgttcg gcggcatgcg
     1501 cgcgatcatg gccacagcga cgccacaggc cgtcatcatc ctgttcggtg ctttcaccat
     1561 cacctggctt ggtctgacga cgctgggcga gggagcaggc gtcatcgcgg gctggggtga
     1621 actcgtctcg acagtgcagg tgaacaagga gcagttcgcc ctgtggcgcc ccttgagcga
     1681 cccgaacttt ccctggctgg gcgtaatgat cgcctctccc atcgtgggca tatggtactg
     1741 gtgtaccgac cagtacatcg tgcagcgtgc gctctccgcg aaaaacctga cgacggcgcg
     1801 acgcggtgcc ctgttcggcg gcttgctgaa ggtgtggccg gtgttgattt tcctggtgcc
     1861 gggactgatc ggctggtcgt tacacgagaa agggctcatc gaactgccca tgcgcatggt
     1921 caacggcgtc tctgagatca acggagacac ggtatttccg gcgctggttt cgacgcttct
     1981 tccctctggc atccgcgggc tgatcgtcgc gtgcctgctg gctgcgctca tgagttcgct
     2041 ggcctcgctg ttcaactcca gcgcatcgct gttcactgtg gacgtgtacg aaaagttacg
     2101 gccgggccag accgagaagc acctgttgct ggtcggccgc atcgctacca cggtggtggt
     2161 cgggctcggt atgctgtgga tcccggtaat gaaggtgatt tcgggcggcg gcctgtacca
     2221 gtacctgcag agcgtccagg gttacctcgc gccgccgatc accgcggtat tcctgctcgg
     2281 gttgttctgg acgcggctca acggcagggg cgccgtttgg gcgctggtgg gcggattcgt
     2341 gctcggcatg ggcaagctga cctgccaggc gttgttcggc gaaggcaaga tcgaaagtcc
     2401 ggcaatactc gcggcgattg gcgatttcaa cttcctttat gcgaccggcg tcctgttcgt
     2461 ggcgagtgcc gccctgatga tcatcggttc actcaccagt gcgccgccgc cggcggaaaa
     2521 aatcgacggg ctcacctatc gttcgatacg cgtgatgcac ggtgacgaga tccgcgcgag
     2581 ctgggatgcg ggcaacaagt tcatggcgac cgccatcctc gtcctggttg cgggaattta
     2641 cctgtacttc agtttctggc tgaattgaat aaggtcaggt gcctttcttc ctgatcctgc
     2701 gcgagttcgt agtatcgagg aaggccaggt ccacgagatt cgccatcgcc gcatgcgcgg
     2761 cctcggggtc tgcggacttg acggcgtcat agacgctctt atggtctggc accgggtcgc
     2821 gcgacagggg gttgttgcgt tgtttgaaca ccgtggtcca ggatatagcc gcggctatgc
     2881 cgctggtcag ggacacgagg aaggcgttgc cgctcgcgcg aagcagtgca gagtgaaact
     2941 cctggtcggc cagccggcct gcttctaccg cgagggtgtg tgtcgccatc ccgtcgagtg
     3001 ctctcgccat cgcatcgagg tcctcgtccg tgcgacgcag ggcagcgagg gcggcggcct
     3061 ccggttccac gatcttccga agctcgaaca ggttctcgag cagcccttcg tccggttcga
     3121 actcgaaaat ccacgacagg acgtcggggt cgagcaggtg ccagcggcct ggcgggctga
     3181 cgcgcgtacc gacttttggc cgcgactcga ccaatccctt ggcggcgaga attcgtacgg
     3241 cttcgcggta ggcgccgcgc gatacctgca ggcgatcgct ggcggcgatt tctccgttta
     3301 gcagttcgcc gggcttgtaa cggcccgaga cgatcaggac tcccagatcc cgtgcaatcg
     3361 atccgtgcaa acgaagggag cggcgcctta cccgcgcatt ggtcttaatt gaagcggctg
     3421 gcagtcgtgt aacgcccggg tttgagccgg ttggccggag gatcttcttt tcagattttt
     3481 tgcgtttcat tgcgggcaaa caataccgca ttgatcggca ttcgagcgag gatcgggagc
     3541 ggagcagggc ttgctgccct gccccgaacg ccccttttat agaagaagac cgtaggagcc
     3601 cgccgtgcgg gcgagttcgc tgacgctttg tcgcactcgc ctgcatggca ggctcctaca
     3661 ggtggcagtc ggatcgtcag gggtgcgact ttgtcaccgc ggtagtttgc ttaatccctc
     3721 gcgcgggact tgcccttttc ctcggcctgt cgcgccttct tgagctcctc ccggctgacg
     3781 tcccgcgcct tgtcagaacg ctcgtcctcg ctcgtctttt ctaccgtttc cttcacacct
     3841 ttacgatacc ggcggtctgc ttcgcggttg ccttctccgc ctttgcgttc ctctgtcatc
     3901 tctggttcct ttgcatgtgg attttgaaaa actcgcgcga tcgcgcaggt tttacgatgc
     3961 aaatggcatg ccagaggtgc caacgaacct agatcgttcg taacgtggcc atcaccttgt
     4021 tgacgatggc atcgcaacgg gggccggcga tttcgtgcac cccgataccg gcggcctcgg
     4081 tatagtcgag tatccgctgc cgcatcaggc gacgtgcgcg gtgaaagcgc gtcttgacgg
     4141 tggcttgcgg gatttccagg atatccgcgg tatcgtcgac gctgcattgc tcgacggcgc
     4201 ggagcacgaa gaccgctcga aatgcgtccg ggagctcgtc gatgcactcc tcgagaaggc
     4261 ttcgaagctg catgttggct acttcgcgtt ccggcaagag ctggcgcggt gtcgtcacgg
     4321 acatctccag gacgttgtcg agatctgatt cttccatcgt ttggaagcgg cggttgccgc
     4381 gcagtcgcat cagcgctgca ttgcgggtaa ttcgcgcaag ccacgtgccg agcgccgccg
     4441 gttccttaag gtcgtcaagc cgccggtacg ccgtgatgaa cgcttcctgg acgacatcca
     4501 tcgcttccga gtcgtccgtg acgatgctgc gggcaagacg atacaggcgc tggttgtgat
     4561 gccgcatgat cccctcgaag gactcgaggc tgccggaaag tacctctgcc agcaggctct
     4621 catcactgga acttgacggt gctggtcgca cagccgtatt ggtcacttca attctccatc
     4681 tgtgtcgctg cattcttcgg cggaggcggt aagactgcca agatacgcca ccaggttacg
     4741 ccgctcccgc gcatcggcga cgccggcgaa acccatggtc gtgcccggaa caaccgtcat
     4801 cggcgccgcg aggaagcggt cgagcgtacc ggcatcccag aggattgcag cttttcgcat
     4861 ggcttcgctg taatcatagc cgggaagcgt gccggaggtg cgcccgaaca gcccgcaatg
     4921 ccgcggcccg gtacggttcc ggtcagggga atggcatcca gtgcatttcg cgtagctccg
     4981 cctgcccgcc gccgcgtcgc cggcagcagg cgccgccgcg acgagcaggg tgaaaaaccc
     5041 gaggcacgtt gcggctgcga cagcagtgcc ggccgcaatt ttcatcgaca aaaccggtta
     5101 tcaggaaaag aacgcgcccg gcaccgggtc cagccccagc gcgttacgca gaaccgccca
     5161 atgcatcgcc tcgtcggcca gtatgctcgc cgcggccgag gacaggtcgc ggttgccgaa
     5221 gagcgggata gcgccggcgt aggcactgac ggcgcctcgc tccagtcccg cagcgaactg
     5281 cagtacgtcc gactgcgcct tcagtttgct gaccgggaaa tcgtacttct gaagtgcgtg
     5341 cgcatccttg cccccgaggc cgcgcaccgc cttggccaaa gcctccacgt gttgcttgtg
     5401 gtgatcctgg aaagcaagtg ccaccttcgc gacctcgggc tgcagcagcc cgctgtcggc
     5461 gcccagctgg taaggggcga cggcttcgtg ctccggcgca atcggcgtat tgagtatgcg
     5521 aacgtcctgc gacacagcat cgtcggcggt tccggctgcc agcgcggtgg agcgcccgcc
     5581 gaggagcgcg atcgccaggc ctgagaatgt cgcggtacct gcctgttgca ggaagcgccg
     5641 gcgggagtcc tttgccgtct cggcgagaag ttgaaagtcg gaagcctgcc tgatcgtttt
     5701 cat
//
LOCUS       DASUOM010000518         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1747232,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000518 DASUOM010000000
VERSION     DASUOM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747232"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..2250
                     /locus_tag="VFY27_10805"
     CDS             46..2250
                     /locus_tag="VFY27_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766047.1"
                     /translation="MRGNVTTRFIAAGVAVVATVAGPEGTANAQAQERSILEEIVVTA
                     QRREENIQDVPISVTAVRGEQLETLFAGGEDVRALAARVPSLYAESSNGRLAPRFYMR
                     GLGNSDFDLAASQSVSVVVDEVVQENVILKSFPLFDLDRVEVLRGPQGSLFGRNTPAG
                     IIKFETRKPTAELDGYAAATVGSLGTLNFEGALGGALNESETLLARLSVLSQNRDDWI
                     DNGFTGESDVMGSFSENAFRLQLLAQPSDAFSALFNVHGRDLNNGTASVFRANILDPG
                     SNSLNENFDRDKVFFDGGNNNPQEARALGGSVRLDFDFGNEVTLTSISSYETVENRSL
                     GDIDGGNLVTGPGFIPFPSETQDGLEDLEQLTQEFRLASDANDRLFWQAGLFWFDSDF
                     SVQTWPFFVPPTTVRHENQSWAAFGQLSFDLGDRTTLTAGVRYTDDDKDMTTPETPNP
                     QPPVSVSDEQVSWDLSLSFAMSDDVNIFGRVASGFRAPSIQGRNIAFFDPNPFSIANS
                     ETILSTEIGFKSTPTDQFRLNGSVYYYTIDDQQLSAIGGNTNSNVLVNADKGKGMGVD
                     LDAELLFSDNFVMTFGGSYNDTEIDDPNLVTAVCAQCTVLDPLDTNGFAILDGNPFPQ
                     APEFMINATARLSASAGDNDEVYLFADVVHQGDTNFFLYESAEYHSGDIFEAGLRAGY
                     IANDGQWELSVFARNITDEENLKGGIDFNNLTGFVNEPRIFGLTFQMNFGEF"
     gene            2295..3200
                     /locus_tag="VFY27_10810"
     CDS             2295..3200
                     /locus_tag="VFY27_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766048.1"
                     /translation="MLLTISGCATADATSPVLMGRIESAEVTEASGIARSTIRSDRFW
                     VVNDGGSPPVLHAVGLDGRDQGSLRLEAASNLDWEAVASFELRGKSWLLVADAGDNTA
                     NRDHSSIYVVEEPALEDGEDAVASPEWTMSFRWPDGPRDCEAVAVDTGAERILLLSKR
                     TIPALLYELPLRPDTAGVITATRLGEVDALPQPTARDLERAAPERNWHWQPTAMDITA
                     DAGAAVILTYRAVYHFERRSGEAWVEALRKRPTIINLGDVREAEAVAFAEDGRSVIVT
                     VEAPSPPLYRVPLLSASRELRREEE"
     gene            3197..4297
                     /locus_tag="VFY27_10815"
     CDS             3197..4297
                     /locus_tag="VFY27_10815"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5766049.1"
                     /translation="MKSMQKRVLPALLITLFLGAGCTSPTTTLDHAEEGQGVTIMTFN
                     VENLFDTRDDPGKDDRTYLPLEAKQTPEHKRACAQVEVATWRDQCLDWDWNEDILDRK
                     MSSVAAAVLQVNDGRGPDILALQEVENVRVLEQLRTRYLADAGYRPAILVEGDDNRGI
                     DVAFLTRLAISGKPRLHRIPFDNAEPSRVADTRGILEATFILPDGTLLAGYAVHFPAP
                     FHPTPMRVAAYEFLAKLRASLPADRPAFAAGDFNTTSEEDRKQSMLDRLARPYFSIVH
                     EKNCRDREGSCRGTSYYAGDDTWSFLDMILWSPARDRSARATWRLRGNSVRIANGTEE
                     QVRDDGTPARFVLPAGSGVSDHWPLHFTIEPT"
BASE COUNT          928 a         1468 c         1370 g          874 t
ORIGIN      
        1 aagaacgagg ttatactgcg cccagtgttt tcagggagca aacaaatgcg tggcaatgtt
       61 acaacccggt tcatagccgc tggcgtcgct gtagtcgcga ctgtcgccgg ccccgaaggc
      121 actgcaaatg cgcaggccca ggaacgttcg atactcgaag agatcgtcgt gaccgcgcag
      181 cggcgcgagg aaaacatcca ggacgtgcca atctcggtaa cggccgtgcg tggcgaacag
      241 ctcgagacgc tgttcgccgg gggcgaagat gtgcgcgcgc tggcggcccg tgttcccagt
      301 ctatacgcgg agtcatcgaa cgggcgactg gcaccccgct tctacatgcg cgggctgggc
      361 aattcggact tcgatctcgc agcctcgcaa tcggtttcgg tcgttgtcga tgaagtcgtt
      421 caggaaaacg taatcctgaa gagtttcccc ctgttcgacc tcgaccgcgt cgaagtcctg
      481 cgtggacctc aaggttcgtt gttcggccgc aatacccctg ccggcatcat caagttcgag
      541 acccggaagc ctactgcgga attggatggg tacgccgccg cgaccgtggg cagcctgggc
      601 acgctgaatt tcgaaggagc gttgggcggc gctttgaacg agagcgaaac cctgctcgcc
      661 cgtctttcgg tactgagtca gaatcgcgac gattggatcg ataatggctt taccggcgaa
      721 agtgacgtga tggggagctt cagcgaaaac gcctttcgcc tccagctgct cgcgcagccg
      781 agtgacgcgt tcagcgcact gttcaacgta cacggccgtg acctgaacaa cggcaccgcc
      841 tccgtattcc gcgccaacat cctggatccc ggcagcaaca gcctcaacga gaacttcgac
      901 cgcgacaagg tctttttcga cggcggcaat aataatccgc aggaagcgag agctctcggc
      961 ggctccgtcc gcctcgactt tgatttcgga aacgaggtga cgcttacttc gatcagctcg
     1021 tatgagaccg tcgagaatcg cagcctcggc gacatcgatg gcggcaacct ggtcacggga
     1081 ccaggattta tcccattccc ttcggagacg caggacgggc ttgaagatct cgagcagctt
     1141 acgcaggagt tccgcctggc ctctgacgcc aacgaccgcc tgttctggca ggccggctta
     1201 ttctggttcg attccgattt ttcggttcag acatggccgt tcttcgtccc gcctacgacc
     1261 gtgcggcatg aaaaccagtc gtgggcggcc ttcgggcagc tcagtttcga cctgggcgat
     1321 cgcacgaccc tgactgccgg cgtccgctat accgatgacg ataaggacat gactacgccg
     1381 gaaaccccca atcctcagcc gccggtgtcc gtctcggacg agcaggttag ctgggatctc
     1441 agcctctcct tcgctatgag tgacgacgtg aatatcttcg gccgtgttgc aagcggtttc
     1501 cgcgcaccgt ccatccaggg ccgcaacatc gctttcttcg acccgaaccc gttttcgatc
     1561 gcgaattcgg aaacgattct gtcgaccgaa ataggcttca agtccacacc aacggaccag
     1621 ttccgcctta acggctcggt ctattactac acgatcgatg atcagcaact ttccgctatc
     1681 ggcggcaaca cgaattcgaa cgtgctggta aatgcggaca agggcaaggg catgggtgtc
     1741 gatctcgacg ctgaactgct cttctccgac aacttcgtca tgaccttcgg tgggagctac
     1801 aacgacacgg aaatcgatga cccgaatctg gtaaccgccg tctgtgcaca gtgcaccgtg
     1861 cttgacccgc tggacactaa cggcttcgct atcctcgacg gcaatccttt cccgcaggct
     1921 cccgagttca tgatcaatgc cacggcgcgg ctgagcgcat cggccggcga caacgacgag
     1981 gtttacctgt tcgccgatgt cgtgcaccag ggcgacacga atttcttcct ttacgagtcg
     2041 gccgaatatc acagcggtga tattttcgaa gcgggcctgc gcgccgggta catcgccaat
     2101 gacggccagt gggagctttc ggtcttcgcc cgcaacatca ccgacgaaga aaacctgaaa
     2161 ggcggcatcg acttcaacaa cctgacgggc ttcgtcaacg agccgcgcat cttcgggctc
     2221 accttccaga tgaacttcgg ggagttttag aaagagccgc aattccgttt tgcgtgggct
     2281 cctacagcgc cctgttgctg ctcaccatct ccggctgtgc gaccgcggac gcgacgagtc
     2341 ccgtcctcat ggggcggatt gaaagcgcgg aggtcaccga ggcgagcggc atcgcacgct
     2401 cgaccattcg cagcgatcgc ttctgggtcg tcaatgacgg aggctcgccg ccggtgcttc
     2461 atgccgtcgg ccttgacggc cgcgatcaag ggagcttgcg gctcgaagcg gcaagcaacc
     2521 tcgactggga agccgtcgct tctttcgagc tccgcggcaa gtcctggctg ctggtcgcgg
     2581 acgccggcga caacaccgcg aaccgcgacc acagctcgat ctacgtcgtg gaagaaccgg
     2641 cgctggaaga tggcgaagac gcggtcgcct cacccgaatg gacgatgagc tttcgctggc
     2701 cggatggccc tcgcgattgc gaagccgttg ccgtggatac cggggccgaa cgcatcctgc
     2761 tgctctcgaa acgcaccatc cccgcgcttc tttacgagct tccgcttcgc ccggataccg
     2821 caggcgttat caccgcgacg cgcctcggcg aagtcgatgc gcttccccag ccaaccgcgc
     2881 gcgacctcga gcgtgcggca cccgagcgca actggcactg gcagccaacc gcgatggata
     2941 taaccgcgga tgccggggcc gcggtcattc tgacctaccg cgcggtgtac catttcgaac
     3001 gaaggagcgg cgaagcgtgg gtcgaggcac tgcgaaagcg gcccacgatc atcaacctcg
     3061 gagacgttcg cgaggcggag gccgtcgcgt tcgccgaaga cggcaggtcc gtaatcgtca
     3121 cggtagaagc tccgtccccg cctctctacc gggtgccgct attgtccgcg tccagggaat
     3181 tgagacgaga ggaagaatga aaagcatgca aaagagagtc ttgcccgccc tgctcatcac
     3241 tctcttcctg ggggccggct gcacctcgcc aacaacgacc ctcgaccatg cagaggaggg
     3301 gcaaggcgtt actatcatga ccttcaatgt cgagaacctg ttcgataccc gggacgaccc
     3361 cggcaaggat gaccgaacct atctccctct cgaggccaag cagacccccg agcacaaaag
     3421 agcctgtgcg caggtggagg tcgccacctg gcgggatcag tgcctggact gggactggaa
     3481 cgaggacatc ctcgaccgca agatgtcgtc cgtcgcggct gccgtcctgc aagtgaatga
     3541 cggcagggga ccggacatcc tcgcgctgca ggaagtcgag aacgtgaggg tcctggagca
     3601 gctgcggacg cgctacctgg ccgatgcggg ataccggccc gcgatcctgg tcgagggcga
     3661 cgacaatcgc ggcatcgacg tcgccttcct caccaggctc gcgatttcgg gcaagccaag
     3721 gctgcaccgc atcccgttcg acaacgcgga gccttcgcgc gtagcggaca cgcgcggcat
     3781 tctggaagcc acgttcatcc tgccggacgg tacgctcctg gccggttacg cggtgcattt
     3841 tcccgcgccg ttccatccca cgcccatgcg ggtcgcggcc tacgaattcc tggcaaagct
     3901 gcgcgcttcc ctgcctgccg accgaccggc gttcgcggcc ggcgacttca ataccacgtc
     3961 cgaggaggac cgcaagcaat cgatgctcga ccgactcgcg aggccctact tcagcatcgt
     4021 ccacgaaaag aactgccggg acagggaggg cagctgccgt ggcacgagct attacgcagg
     4081 cgatgacacc tggtctttcc tcgacatgat cctgtggtca cctgcccgcg accgtagcgc
     4141 gcgcgcaaca tggcgtttac gcgggaactc ggtgcgcatt gcgaacggga cggaagagca
     4201 ggtgcgtgac gacggtactc cggcacgctt cgtgctgccg gccggcagcg gtgtgtccga
     4261 ccactggcct ctgcacttca cgatcgaacc aacatagaaa caacgccttg cagcgtcact
     4321 tgcgcacgcc tggtgagctt cggcgcactg cagagcgccg agcggcggga ttttctgcgt
     4381 ctccttcggc tgaaaccctt attgcatggg catttggcct gcgatagatt tgccgcacca
     4441 caacaggaac gggcaagccg gatccttaag taatgctgaa aatgttcgac aaccaatgcc
     4501 ggggcgaaac ctgactgtca caacttccta ttagcattcg ctcctctatc actcccggga
     4561 atccacaatg agaaacaaga tcggccacgt acgtccagtc gctggactct ggtacgcgtt
     4621 tgccagcgcc ctgttcgttt
//
LOCUS       DASUOM010000519         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_900403,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000519 DASUOM010000000
VERSION     DASUOM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900403"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFY27_10820"
     CDS             <1..1184
                     /locus_tag="VFY27_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766050.1"
                     /translation="QREIAHMRSFVDRFRAKASKARQAQSRIKALERMEIIAPAHVDS
                     PFHFSLRPPEKLPNPLLRMDDASAGYGGAPVLNSVRLTLTPGDRIGLLGANGAGKSTL
                     IKLVAGMLEPLEGERVPANDLNIGYFAQHQVEQLYPERSPLEHFFEIEPTAREMDLRN
                     YLGGFGFPSDTVFMKTGPFSGGEKSRLALALLVYRRPNLLLLDEPTNHLDLEMRQALS
                     VALQEFPGAMMIVSHDRHLLRVTTDQLLLVHAGSVDEFDGSLDDYPAWLAAQNRAGAK
                     PGSNPDRRSGTATTESLRERKDRKRREAAERQSFVRFNRRVEKAEAVLAEAHERKKQL
                     EERLADTTLYQPKNKEKLQAVLAEQVDHNWALAKAEAEWLEATEALERVQAPEQRTVT
                     P"
     gene            1383..>3143
                     /gene="recQ"
                     /locus_tag="VFY27_10825"
     CDS             1383..>3143
                     /gene="recQ"
                     /locus_tag="VFY27_10825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX5766051.1"
                     /translation="MTDDIHRILREVFGFTRFQGKQEAAIVSALAGRDSLVLMPTGGG
                     KSLCYQVPALLREGVAVVVSPLIALMQDQVAALIESGIQAAYLNSTLSYAEQQEVLAN
                     LREGRLKLLYVAPERLLQEQTLSLLTTLPVSLIAIDEAHCVSQWGHDFRTDYLALNEL
                     ARLFPGIPRMALTATATAPTRKEIVERLALKDPQMFLSGFDRPNIRYLVQAKSDARRQ
                     LLQFLDRHPDEAGIVYCLSRKKTESTAEWLQSRGLKALPYHAGLPGEVRAAHQRRFLV
                     EDAVIIVATIAFGMGIDKPDVRFVAHLDLPKSIESYYQETGRAGRDNRPADAWMVYGL
                     QDVVRLRQMMDESAAAEEYKRNERQKLDSLLGWCEVTTCRRRSLLEYFGDRHDHDCGN
                     CDVCLTPPVTWDGTEASRKLLSAVYRTGQRFGAAHVVDVLLGKATEKVLQHRHDSISV
                     FGIGKEFEAPAWRSVMRQLIVQGYLRADPERFGALRLAEKSRPLLRGETTVMLRRDLS
                     LGAPLKKPRTARTAVAEADRAFWEALRGCRQLLAAEHKVPPYVIFHDATLMQIVDRRP
                     RNEHELLGISGVGQAKLQRYG"
BASE COUNT          618 a          962 c         1008 g          555 t
ORIGIN      
        1 agcagcgcga gatcgcgcat atgcgatcct tcgtcgatcg cttccgcgcg aaggcgtcga
       61 aagcacgaca ggcgcagagc cgcatcaagg ccctggagcg catggaaatc attgcaccgg
      121 cgcatgtcga ttcgccattc cacttttccc tgcgcccgcc tgagaaactg ccgaatcccc
      181 tgttgcgcat ggacgacgca tctgccggct acggtggtgc gccggtcctg aacagcgtgc
      241 gtctcactct tacgccgggc gatcgcattg gcttactcgg cgccaacggc gcgggcaagt
      301 ccacgctcat caaactggta gcgggaatgc tcgagcccct ggagggggag cgggttccag
      361 cgaacgacct gaacatcggc tacttcgcgc agcaccaggt ggagcagctt tatccggaac
      421 gctcgccact ggaacatttt ttcgagatcg agccaacggc gcgcgagatg gatctgagga
      481 attatctcgg cggtttcggc tttccaagcg atacggtatt catgaagacc ggcccgtttt
      541 caggcggtga gaaatcacgg ctcgcactgg cgctcctggt ttatcgccgc cccaacctgt
      601 tgctcctgga cgaacccacc aaccacctgg atctggagat gcggcaggcg ctgagcgtcg
      661 cgctgcagga gttccccggt gcgatgatga ttgtctccca cgaccggcac ctgctccggg
      721 tgacgacgga ccagcttctc ctggtgcatg ccggttcggt agacgagttt gacggcagcc
      781 tcgatgatta cccggcgtgg ctcgcggcac agaaccgggc cggcgcaaag cccggcagta
      841 atcccgacag gcgcagcggg actgcaacta ccgagagtct cagggagcgc aaagatcgca
      901 agcgtcgtga ggcagccgag aggcagtctt tcgtgcgctt caatcggcgg gtcgagaaag
      961 ccgaggcggt gctggcagaa gcgcacgagc ggaaaaagca gctggaagag cgcctggcgg
     1021 ataccacgct ttaccagcca aagaacaagg agaagttaca ggcggtcttg gcagaacagg
     1081 tggatcacaa ctgggcgctg gcaaaagcgg aggcggagtg gctcgaagcc accgaggcgc
     1141 tcgagcgggt ccaggcgccc gagcaaagga cggtgacgcc atgaagccgg tcgaccgacc
     1201 acttcgcggt acgtgcgagc acgggcgaag tcggtgctta caactgcgca aagcctgcgc
     1261 gaccgccatg cgcgccgcac ttcaggtggc cgatgagatc ggacgtctgc gccattcttt
     1321 tcccttgttt ttgccaggct tggcctgcgc gcatcctgcc aaaagttctg tatcctgacg
     1381 ggatgacgga cgatattcat cggatattgc gggaggtatt cggctttacc cgcttccagg
     1441 gaaagcagga ggctgcgatc gtgagcgcgc ttgccgggcg cgactcgctg gtgctgatgc
     1501 cgacgggtgg cggtaaatcc ctttgctacc aggttcccgc gctgctgcgg gaaggcgttg
     1561 cagtggtggt atcgccgctc atcgcattga tgcaggacca ggtcgcggcc cttattgagt
     1621 cgggaatcca ggcagcgtac ctcaactcga ccctttctta tgcagaacag caggaggttc
     1681 tcgcaaacct tcgcgaggga aggctgaagc tgctttacgt tgcgccggaa cgcttgctgc
     1741 aggagcagac actgtcactg ctcacgacac tgccggtatc gctcatcgcc atcgacgaag
     1801 cccactgtgt ttcgcagtgg ggtcacgatt tccgcaccga ctaccttgca ctgaacgaac
     1861 tcgcgcgcct gttccccgga attccacgca tggcgctgac ggcaactgcg accgcgccta
     1921 cccggaagga gatcgtcgaa cgactggcgt tgaaagatcc gcagatgttc ttgagcggct
     1981 tcgaccggcc gaacatacga tacctggtac aggcaaagtc cgatgcgcgc aggcagctcc
     2041 ttcagttcct ggaccgacat cccgacgaag caggcatcgt ctactgcctg tcacgcaaga
     2101 agaccgagag cactgccgaa tggcttcaat cccgggggct taaggcattg ccgtatcacg
     2161 ccggcttgcc gggcgaggtt cgggctgccc accagaggcg cttcctggtc gaggacgccg
     2221 tgatcatcgt tgcgaccatc gccttcggca tgggaatcga caagcccgat gtgcgcttcg
     2281 tcgcgcatct cgacttgccg aagagcatcg aatcgtatta ccaggaaacc ggcagggccg
     2341 gacgcgataa ccggccggcg gatgcatgga tggtgtatgg cctgcaggac gtcgtgcgcc
     2401 tgcggcagat gatggacgaa tccgccgcgg ccgaggaata caaacgcaac gagcgccaga
     2461 agctcgattc actgctgggc tggtgcgaag tgacgacctg ccgacgtcgc tccctgctcg
     2521 agtattttgg cgatcggcac gaccatgact gcggcaactg cgacgtttgc ctgacgcctc
     2581 ccgtgacctg ggacggcacc gaggcctcgc gcaagctgtt gtcggccgtg tatcgaacgg
     2641 gccagcggtt cggcgcggca cacgtggtcg acgtcttgct gggaaaggcc actgaaaagg
     2701 tgctgcagca ccggcacgac agcatcagcg tgttcggcat cggcaaggaa ttcgaggctc
     2761 cggcatggcg gtccgtgatg cggcaattga tcgtgcaggg ttacctgcgc gccgaccctg
     2821 aacgcttcgg cgcgctcagg ctcgcggaga agagcaggcc cctgctgcgc ggcgaaacaa
     2881 ccgtgatgtt gcgccgcgac ttgagcttag gcgcacccct gaagaagccc cgcaccgcca
     2941 ggaccgccgt ggcggaggcc gaccgggcgt tctgggaggc acttcgcggc tgccgccagc
     3001 tgctcgccgc cgaacacaag gtgccgccct atgtcatatt ccacgatgcg acgctcatgc
     3061 agatcgtcga ccgccgcccg aggaacgagc acgaactgct ggggatcagc ggcgtggggc
     3121 aggccaagct gcagcgctac ggc
//
LOCUS       DASUOM010000520         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1635772,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000520 DASUOM010000000
VERSION     DASUOM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635772"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VFY27_10830"
     CDS             <1..887
                     /locus_tag="VFY27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5766052.1"
                     /translation="LFVEAGGPPGAFNVINGIGEVAGKALALHNDVDKVTFTGSTEVG
                     KLMLQYAGQSNMKRVSLECGGKSPQIFMGDLPDLERAVTAAFNGIYANMGEVCNAGSR
                     LLVDRTIYDEFVDRFVTEGQHAYVPGDPLDPSTNLGPLVTREAQHRVLSMVEAGKKAG
                     AELKFGGTVPEGLQNGAYVTPALFTGVDNSMRIAREEIFGPVAAVIPFDGANEAVSIA
                     NDTIYGLAAGIWTRDLGRAHRMIRDIEAGVIWVNCFDEGDMTQPFGGYKQSGNARDKC
                     LESLLSYTQSKSAWIRLS"
     gene            complement(939..1694)
                     /locus_tag="VFY27_10835"
     CDS             complement(939..1694)
                     /locus_tag="VFY27_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5766053.1"
                     /translation="MRETRPPQGAQAALRAIRLLKLFTAERPEMSLSELSRGSGLNKT
                     TTHRLLRALQSENLIERNAATSAYSLGPGLMALGVQALASSDLRRRVRPLLKALAKSS
                     GETATLEVPIGDSMLILDEVAGTHVIGAVGNVGTRWPLHATSTGKAFLAFDDNGIERL
                     GSPLKALTARTLVDKEAFQPQLIEIRRRGYAMSVDELEEGYAAVATIIRGALGDVQGT
                     LCIGGPTQRLNAARRAELGASLCEAASRLTPDM"
     gene            complement(1813..>3240)
                     /locus_tag="VFY27_10840"
     CDS             complement(1813..>3240)
                     /locus_tag="VFY27_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5766054.1"
                     /translation="RVSTASYIASMLRPEVIRDLKLGAYGLKMIACDPGVQTAMPDGS
                     VISWWSDRERMKLELSEKAPEDREEFFRVDAHLRELASYLQPFFMEPPPDPYRSGAGR
                     LLEVLRISRRFRGLRGEQVASFVRFLTGSLGDFLDRHFHSEAVKRLILSNSLYGKHGG
                     PYQPGTAMGLLFHLLSGGAEERQGFAGHVIGGMGAITQAMAAACRDRGVQIRTGTRVA
                     AINHGGGRVSGVTLEDGSVIDAPIVVSNADPRRTFLEMLSGGLPEDFRRDVAAIKMAG
                     PCAKVNLVLTEEPAVKGMPATHSKTERSLFTLAPSLQEAENSYNEARRGEVPRDLWVD
                     CVLASNVDEGLATPGLHVLTSFVQYLPYRLAKGRWEDEREALGDRVIEIIGRYAPNVP
                     ASVVARRVLTPLDLEETFGITEGNIFHGDISLEQLFFMRPVPGFARYRTPVDGLWLCG
                     AGTHPGGGVTGAPGYNAARCILAGR"
BASE COUNT          621 a         1010 c         1018 g          591 t
ORIGIN      
        1 aactcttcgt cgaggccggc ggaccgcccg gggccttcaa tgtcattaac ggcatcgggg
       61 aagtcgcggg caaggcgctc gcgttacaca acgatgtcga caaggtcacc ttcacgggct
      121 cgacagaagt cggcaagctc atgctgcagt acgccggtca gtcgaacatg aagagagttt
      181 cccttgaatg cgggggcaaa tcgccgcaga ttttcatggg cgacctgccg gatctcgagc
      241 gcgccgtcac ggccgcgttc aacggcatct acgccaacat gggagaagtc tgtaacgccg
      301 gttcacgcct gctggtggac aggacaattt acgacgagtt cgtggatcga ttcgtcacag
      361 agggacagca cgcttacgtg ccgggagacc cgctcgaccc gtcgacgaac cttggacccc
      421 tggtgacgcg cgaagcacag catcgggtgt tgtccatggt ggaagccggg aaaaaggccg
      481 gcgcagagct caagttcggc ggcacggtgc ctgagggcct gcaaaacggc gcgtatgtga
      541 cgccggcgct attcacaggc gtcgacaatt cgatgcggat cgcgcgcgag gaaatcttcg
      601 gtcccgtagc ggccgtcatc cccttcgacg gggcgaacga ggcggtcagt atcgccaacg
      661 acacgattta cggcctcgcg gccggcatct ggacgcgaga ccttggtcgc gcgcaccgga
      721 tgatccggga catcgaggcc ggggtgatct gggtcaactg cttcgacgaa ggcgacatga
      781 cacagccgtt cggcggctat aagcagtcgg gaaacgcccg cgacaagtgc ctggaaagcc
      841 tgttgagcta cacccagtcg aagtcggcct ggatacgcct ctcctgactg gcgtcggcgg
      901 aagaaagtgt ccctgctgct acctgccgcg aaaacctcct acatgtcggg tgtcagcctg
      961 gaggctgcct cacagagcga tgcgccgagc tcggcgcgcc tggccgcgtt cagcctctgc
     1021 gtagggccgc cgatacacaa cgtaccctga acgtcaccca gcgcgccgcg gatgatggtt
     1081 gcgaccgccg cgtaaccttc ctcgagttcg tccaccgaca tggcatagcc acggcggcgg
     1141 atctcgatga gctggggctg gaaggcctcc ttgtccacga gcgtgcgggc agtgagcgct
     1201 ttcagggggc tgccgaggcg ttcgataccg ttgtcgtcga aggccaggaa tgccttgccg
     1261 gtagaggtag cgtgcagcgg ccagcgcgtg ccgacgttac ccaccgcgcc gatcacatgg
     1321 gtgccggcaa cttcgtcgag tatcagcatc gaatcaccga tgggaacttc cagcgttgcg
     1381 gtttcgccgc tgctcttcgc caatgccttc aaaagcggcc gcacgcggcg ccgcaggtcg
     1441 ctgctggcca gcgcctgcac gccaagggcc atcaaaccgg gacccaggct gtacgcgctg
     1501 gtcgcggcat tgcgttcaat caggttttcg ctctgcagcg cgcgaagcag ccgatgggtg
     1561 gtggtcttgt tcaggcccga cccgcggctc agctccgaaa gtgacatttc cgggcgctcg
     1621 gcggtgaaca gcttcaacaa gcggatggcc cgaagcgcgg cctgtgcccc ctggggtggt
     1681 cgcgtttctc gcataacgct gactccatat tatgattgac ggttccatat tatggcagca
     1741 gcgcgctttc atcaaccctc gttccacttc gcccaaaagt ggaatacttg ttctgcgagt
     1801 ggcgagagcg tcctagcgcc cggcgaggat gcagcgcgcg gcgttgtatc cgggcgcgcc
     1861 ggtaacgcct ccgccgggat gagtcccggc accgcacagc cagagaccat caacaggtgt
     1921 gcggtaacgt gcgaagcccg gcacgggccg catgaaaaaa agctgctcca ggctgatgtc
     1981 cccgtgaaag atatttccct cggttatgcc gaaggtctcc tcgaggtcga gcggcgtcag
     2041 caccctgcga gcgacgaccg acgctgggac gttcggagcg taacggccga ttatctcgat
     2101 aacccggtcg cccaaggctt cgcgctcgtc ttcccagcga cctttcgcca gccgataggg
     2161 aaggtactgc acgaaagagg taaggacatg cagccctggc gtagccagcc cttcgtcgac
     2221 gttggaggcg agcacgcagt ccacccacag atctcggggg acctctccgc ggcgtgcttc
     2281 gttgtagctg ttttcggctt cctgcaggga aggcgcgaga gtgaacaggc tcctttccgt
     2341 cttgctgtga gtggcaggca tgcccttcac ggcaggttct tccgtcaata cgaggttcac
     2401 cttcgcgcaa ggtccggcca tctttatcgc cgccacgtcg cgccggaaat cttccggcaa
     2461 tcctcccgag agcatctcga ggaaggttct cctgggatcc gcgttggaga cgacgatcgg
     2521 cgcgtcgatc acggagccgt cttcgagggt aacgccgctg acgcgcccgc cgccatggtt
     2581 tatcgcagca acccttgttc cggtgcgaat ctgcacacct cggtcgcgac atgctgccgc
     2641 catggcttgc gttatcgcgc ccatgccgcc gatgacgtgt cccgcgaaac cctggcgttc
     2701 ctcggcacct ccgctcaaaa gatggaacag gagacccatc gccgtaccgg gctggtaggg
     2761 accgccgtgc ttgccgtaaa ggctgttgga gagtatcagc cgcttgaccg cttcggagtg
     2821 aaagtgccgg tcgagaaaat cgccgagcga gccggtgaga aagcgcacaa acgatgcaac
     2881 ctgctccccg cgcaggccgc gaaagcgacg gctgatgcgc aatacctcga gcaggcggcc
     2941 tgccccgctt ctgtacggat ccggcggagg ctccatgaag aacggctgca ggtaggaagc
     3001 aagctcccga agatgtgcat cgacgcggaa gaattcctcg cgatcttccg gagccttttc
     3061 tgaaagctcc agcttcattc gttcgcgatc cgaccaccag gatatgacgc tgccatcggg
     3121 catcgcagtt tgcacgcccg gatcgcaggc gatcatctta agcccgtaag cgccgagctt
     3181 caggtcgcgt atgacttcag ggcggagcat gcttgcgata taggaagcag tcgacacccg
//
LOCUS       DASUOM010000521         5893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1152153,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000521 DASUOM010000000
VERSION     DASUOM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152153"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFY27_10845"
     CDS             <1..716
                     /locus_tag="VFY27_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HEX5766055.1"
                     /translation="KQFEHYRKKCPDFSADVRRSRNVSAGLMVSNSTLLISEDARVPR
                     ERLDALLHHEIGIHILTWHNGGHQPLRQLQSGLAHYDELQEGLGVLAEYLAGNLSPLR
                     LRTLAARVIAVRQMVDGAAFVDTFNELKTRFGFTGRSAFIIATRVFRGGGLTKDVVYL
                     RGLKRLLAYLREGHAFEQLLIGKFSLSQLPAIRKLDEAGWLQAPKLVPAYTHNAAAQR
                     RLVECTEVPLDGLYQRLVA"
     gene            727..1773
                     /locus_tag="VFY27_10850"
     CDS             727..1773
                     /locus_tag="VFY27_10850"
                     /inference="COORDINATES: protein motif:HMM:NF009110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthetase"
                     /protein_id="HEX5766056.1"
                     /translation="MKIGFVINDIRTEVAAYATVRLARSARNLGHDVWLFGVADFIYD
                     PDGSVHATAATPQKKKYKDNEELLEELQDEDAEYDRILIDELDVLLLRNDPAEDATSR
                     PWAQTSAILFGKLAIERGVLVLNDPSHLAYALNKTYFQHFPEQVRPDTCIARDTGSIR
                     RFIEAHDGSAVIKPLQGSGGQNVFLVQPGEEANLNQMVEAVLRDGYAIVQEYLPAARD
                     GDMRLIVMNGRALQVDGKYAAFRRVNKEGDARSNVKAGGKIQPAEPDERALRLVDMVR
                     PKLVQDGMYLVGLDIVGDKLMEINVFSPGGIGVAERLYEVNFADQIIADLERKTLTRS
                     YYGHRLTNAQLATL"
     gene            1865..2839
                     /locus_tag="VFY27_10855"
     CDS             1865..2839
                     /locus_tag="VFY27_10855"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEX5766057.1"
                     /translation="MTMRGAWLSVVALSITRAASADALIDRDNGPLSGLFSFPDSREG
                     SQLAAEGDSRWELSASVSSHSTRDSGGGESLLFDGETARFGVTWRRGITERLELGVEL
                     PWVLHESGNLDSFIDRWHDIFSLPDGIRDERPHDRLLFRYGDGTRLLTLQRNVGGPGD
                     VRLLGGWQLTGGTSGSSALRLSVKLPTGDSDSLLGSGSTDISLGFASDRSRLLGAERL
                     SGFYRINATWLGAHDIGIRRNSSLVGQASGGISHALTPRTSLALQALVRSPVYDSDVS
                     PLGDFAASVTMGVRFRLPHGYSLAFSVGEDIHPHSMPDVTFGMQLQAR"
     gene            complement(2916..4361)
                     /locus_tag="VFY27_10860"
     CDS             complement(2916..4361)
                     /locus_tag="VFY27_10860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5766058.1"
                     /translation="MIEKNAQRFLPVAALTAAALLASPPGVTQDPADNQPNPETQARE
                     APPPGGPPKDFALPERDVRQLDNGLKMTTVQFGAVPKATISVIVSAGNLNEGDRTWLA
                     DLTGDFLLEGTTSRTAEDIALQAAAMGGQVDVTVTEDLTTITGDVLTEFAPEMARLLA
                     DIVRNPAFPEGELERLRRDRLRTLSVAETEPDQMAMAAFREALYGKHPYGQLFPEEEE
                     LQSYDIDAVRGFYEGNFGAKRTHVIVAGKFDDQETVGAVEEAFADWKAGPDPLIDIPQ
                     PAQGKVVVDVIDRPDASQSNIYLGLPTIAPGHEDWIPLQVSNTLLGGFFSSRITRNIR
                     EDKGYTYSPFSTLSTRYKDGYWAQVAAVTTEVTGPAIQEIVNEIDELQENPPPAQELE
                     GVKNYSAGVFVLQNSTRGGIINVLSYIDHHELPESYLTNYVSEVHAVTPEQVSEIARE
                     YLREEDMTLVVVGDRSQVSEQVAPFIGAREE"
     gene            complement(4354..5811)
                     /locus_tag="VFY27_10865"
     CDS             complement(4354..5811)
                     /locus_tag="VFY27_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5766059.1"
                     /translation="MKKQFGILPAVFLLLLAAACERAPEPAEEATDARDTQQEQGSDA
                     RSLFSVPVEYHTLDNGLKVVMSQDRTAPLVTVAVYYNIGFRIEPRDRTGFAHLFEHMM
                     FQGSKNLGKMEFVNLVQRNGGVLNGSTRFDYTNYFEIVPSNKLETMLWAEADRMRSLD
                     ITEDNLVNQQGVVKNEVKVNVLNTPYGGYPWLDMPQYANTNWYNAHNFYGELDDLDAA
                     SLDDVADFFSRYYAPNNAAIAIVGDFESEVALAMVREHFGDIKPSPAPIELPDISEPR
                     QEEQKTASRRDPLATRPGFAFAYHAPPRLTKEWYAMGLLDQVLLQGEDSLLHQALVKR
                     EGITSSVDGGINAFLGNMLNYKGPMLWQAFFIHDQNVDQERIYTALDEVIERVRTQPV
                     DAETLARAKVKWRSGYYDTVTDNFGFGRADLLASLALFDDKPQLINEFEAEILDVTPE
                     LMLQTAREYLRPTNRTVLTLEAGAGTDGEEVASND"
BASE COUNT         1057 a         1791 c         1844 g         1201 t
ORIGIN      
        1 tgaagcagtt cgaacactat cgcaagaagt gccctgactt ttcggccgat gtacggcgtt
       61 ccaggaacgt gtctgccgga ttgatggtgt caaacagcac gctgctcatc tccgaggacg
      121 cgcgggttcc tcgcgaacgg ctggatgcgc tcctgcatca tgagatcggc atccacatac
      181 tcacctggca caacggcggc caccagccac tgcgacagct ccaatccggc ctcgcgcact
      241 atgatgaact tcaggaaggc ctcggcgtcc tggcggagta cctggcgggt aacctgtctc
      301 cgttgcgcct gcggacattg gcggcacgcg tcatcgcagt gcgccaaatg gtggatggcg
      361 cggcgttcgt cgacacgttc aatgaactga aaacgaggtt cggcttcacg ggccgttctg
      421 cgttcatcat cgcgaccagg gtattccgcg gcggcgggct gaccaaggac gtagtgtatc
      481 tgagaggcct caagcggctg ctcgcgtacc tgcgggaggg gcatgccttc gagcagttgc
      541 tgatcggcaa gttttcgctg tcgcagcttc cggcgatccg caagctggat gaagcaggct
      601 ggctgcaggc gccgaagctg gtccccgctt acacccacaa cgcggcagcg caacgccgcc
      661 ttgtcgagtg cacggaggtc ccgctggacg ggctttacca gaggttggtg gcatgagggc
      721 catggcatga aaattggatt cgtcatcaac gacattcgca cggaggtcgc agcatacgcg
      781 acggtgcggc tggcgaggtc cgcgcggaac ctcgggcatg acgtgtggct gtttggcgtc
      841 gcggacttca tctacgaccc agacggttcc gtgcatgcca ctgcggcgac gccacagaag
      901 aagaaataca aggataacga agagctcctg gaagaactgc aggatgagga cgcggagtac
      961 gatcgcatac tcatcgacga actggatgtc ctgctcctgc gcaacgatcc agcggaagat
     1021 gcaaccagca ggccgtgggc gcagacgtcc gcaatcctgt ttggcaagct tgcgatcgag
     1081 cgcggcgttc tcgtattaaa cgacccgagt cacctggcgt acgcgctcaa caagacttac
     1141 ttccagcact tcccggagca ggtacgcccc gacacctgta tcgcgcgcga caccggcagc
     1201 atccggcgct tcatcgaggc gcacgacggg agcgctgtga tcaagcctct gcaaggttcc
     1261 ggaggacaaa atgtgttcct cgtgcagccg ggagaagagg ctaacctgaa ccagatggtg
     1321 gaagccgttc tgcgtgacgg ctatgccatc gtgcaggagt accttcccgc cgctcgggac
     1381 ggggacatga gactgatagt aatgaatggc cgtgccctgc aggtcgatgg gaagtacgcc
     1441 gcctttcgcc gcgtcaacaa ggaaggcgac gctcgcagca acgtcaaggc cggcgggaag
     1501 atccaacccg cagagccgga cgagcgtgcg ctgcggcttg tggacatggt gcgaccgaaa
     1561 ctggtgcagg atggaatgta cctggtcggg ctggacattg tcggcgacaa gctgatggag
     1621 ataaacgtgt tctcgccagg agggatcggc gtcgcggaaa gactttacga ggtcaatttc
     1681 gccgaccaga tcatcgcgga cctcgagcga aaaacgctga cccggtcgta ctatgggcac
     1741 cggctgacga atgcgcaact tgcgacattg tgaggcgccg cagggcagga tataacctgc
     1801 cctttcgaag aacaatttga atgtccactg attgcagtcg ctgccatccc cgcgatgcag
     1861 ccgcatgacc atgcgcgggg cctggctttc agtcgttgcc ttgtcgataa ctcgcgccgc
     1921 atcggccgat gcgttgatcg accgcgataa cggcccgctg tcgggactgt tcagctttcc
     1981 cgacagccgc gaagggagcc agctcgcggc cgaaggcgac agccgctggg agctgtccgc
     2041 ctctgtctcc agtcattcga ccagggactc cggcggcggg gaatcgctgc tattcgacgg
     2101 tgagacggcc cgcttcggcg tgacctggcg ccgcggcatc acggagcggc tcgaactcgg
     2161 cgtcgagctc ccgtgggtgc tgcacgagtc cgggaacctc gattccttca tcgaccgctg
     2221 gcacgacatc tttagcctgc cggacggcat ccgcgacgaa cgcccgcacg accgcctgct
     2281 gtttcgctat ggcgatggta cccggctgct gacactgcag cggaatgttg gcggccccgg
     2341 cgatgtccga ttgctgggcg gttggcagct cactggcgga acgagcggca gttcggcgct
     2401 gcgcttgagc gtcaagcttc ccacgggcga cagcgattca ctgctcggca gcggcagtac
     2461 cgatatcagc ctggggttcg ccagtgatcg cagcaggctg ctgggtgcgg agcggctttc
     2521 cggcttctat cgaataaacg ccacgtggct gggggcgcat gatatcggga ttcgacgcaa
     2581 cagctcgctg gtcggtcagg catccggcgg gataagccat gcgctgacgc cgcgcacgtc
     2641 tctcgcgttg caggcccttg tccgcagccc ggtttacgat tccgacgttt cgccactcgg
     2701 ggatttcgcc gcctcggtga cgatgggggt gcgttttcgc ctgccgcacg gctactcgct
     2761 cgcgttttct gtcggtgaag acattcaccc gcactcgatg ccggacgtga cgttcggcat
     2821 gcagttgcag gcgcgataac tttctcctcc tacaagtgct gccgcgaagc ccggcataat
     2881 gtcgcgtctc ggcgaggcgc gggtcctaca gactgctatt cttcgcgcgc gcctatgaac
     2941 ggggcgacct gctccgagac ctggctgcgg tcgcccacga ccaccagcgt catgtcttcc
     3001 tcgcggaggt attcgcgcgc gatttcggag acctgttccg gtgtcactgc atgcacctca
     3061 ctgacgtagt tggtgaggta gctttccggc agctcgtgat gatcgatata gctcagcaca
     3121 ttgatgattc cgccacgcgt cgagttctgc aggacgaaaa cgcctgcact gtagttcttg
     3181 acgccttcca gctcctgggc cggcggagga ttctcctgca gctcatcgat ctcgttgacg
     3241 atctcctgta tagcgggacc tgtcacctca gtggtgacgg cggctacctg cgcccagtaa
     3301 ccgtccttgt agcgcgtcga gagcgtgctg aacggcgaat aggtatagcc tttgtcctcg
     3361 cggatgttcc gggttatgcg cgacgaaaag aaaccgccga gaagcgtgtt actcacctgc
     3421 agcggtatcc aatcctcatg tcccggggcg atcgtcggca agccaaggta gatgtttgac
     3481 tgggatgcat cgggccggtc gatgacgtcg accaccactt tcccctgggc gggctgcgga
     3541 atgtcgatga gcggatcggg gcctgctttc cagtctgcaa aggcttcctc gactgccccc
     3601 accgtctcct ggtcgtcgaa cttgccggca acgattacgt gcgtgcgctt cgcgccaaag
     3661 tttccttcgt agaagccccg cactgcgtcg atgtcgtaac tttgcagttc ttcctcttcc
     3721 ggaaacagct ggccgtatgg atgtttgccg tacaacgcct cgcggaatgc ggccatcgcc
     3781 atctggtccg gctcggtttc ggccacgctc agcgtgcgca agcggtcgcg acgcagccgc
     3841 tccagttcac cttccgggaa ggccgggttg cggacgatgt cggccagcag cctcgccatc
     3901 tccggcgcga attctgtcag cacatcaccg gtaatcgtgg tcaggtcttc ggtcactgta
     3961 acgtcgacct ggccgcccat cgcggccgcc tgcagcgcga tatcctcggc agtccgggac
     4021 gtcgtgcctt cgaggaggaa gtcgcccgtg agatcggcaa gccaggtgcg atctccctcg
     4081 ttcaggttac cggcgctgac gatgaccgaa atagtggctt tgggaaccgc gccgaactgc
     4141 actgtcgtca tcttcaggcc gttgtcgagc tggcggacgt cccgttccgg cagggcgaag
     4201 tctttcggcg ggccgccggg cggaggcgct tcccgggctt gcgtctcggg gttcggttga
     4261 ttgtcggccg gatcctgcgt cactccgggc gggctggcga gcaaagcggc cgcggtaagc
     4321 gccgcgacgg gcaaaaagcg ttgcgcattc ttctcaatca ttgctggcca cctcttctcc
     4381 atcggtaccc gcgcccgcct ccagcgtcag tacggtccgg tttgtcggac gcaggtattc
     4441 ccttgccgtt tgcagcatca gctcgggcgt gacgtcgagg atttccgcct cgaactcgtt
     4501 tatgagttgc ggcttgtcgt cgaacagcgc gagcgacgcc aggaggtcgg cccggccgaa
     4561 gccgaaatta tcggtcaccg tgtcgtagta accggaacgc cacttgacct tcgcgcgggc
     4621 aagcgtttcg gcatcgaccg gttgcgtccg aactcgttcg atgacctcgt cgagggccgt
     4681 gtaaattcgc tcctggtcca cgttctggtc atgaatgaaa aaggcctgcc agagcatggg
     4741 gcccttgtag ttcagcatgt tgccgaggaa tgcgttgatc ccgccgtcga ccgagctcgt
     4801 tatgccctcc cgcttcacca gcgcctggtg aagcaggctg tcctctccct gcagcaggac
     4861 ctggtcgagc aggcccatcg cgtaccactc ctttgtcagc cgcggcggcg catgataggc
     4921 aaaggcgaag cccggcctgg tggcgagcgg gtcgcgccgg ctggcagttt tctgctcctc
     4981 ctggcgcggt tcgctgatgt ccggcagctc aatcggcgcg ggcgagggtt tgatgtcgcc
     5041 gaaatgctcc ctcaccatcg cgagcgcgac ttcggactcg aagtcgccga cgatggcgat
     5101 tgcggcgttg ttcggcgcgt agtacctgga gaagaagtct gcgacatcat cgagacttgc
     5161 ggcgtccaga tcgtccagct ccccgtaaaa attgtgcgcg ttataccagt tcgtattggc
     5221 gtactgcggc atgtccagcc atgggtaacc gccgtaaggg gtgttcagca cgttcacttt
     5281 tacctcgttt ttcaccaccc cctgctggtt gacgaggttg tcctcggtga tgtcgagact
     5341 gcgcatccgg tcggcctcgg cccacagcat cgtctccagc ttgttgctgg gaacgatctc
     5401 gaagtagttg gtgtaatcga agcgagtcga gccgttgagc acgccgccat tgcgctgcac
     5461 gaggttcacg aattccatct tcccgaggtt cttcgagccc tggaacatca tgtgttcgaa
     5521 caggtgcgcg aagcctgtgc ggtcacgcgg ctcgatgcgg aagccgatgt tgtagtagac
     5581 agcgacggtg acgagaggag ctgtcctgtc ctgggacatg accaccttga ggccgttgtc
     5641 cagcgtgtga tactccacag gcacggagaa caacgatcgc gcatcgctgc cctgttcctg
     5701 ttgagtatcc cgggcatcag tcgcttcttc tgctggctcg ggcgcccgct cgcaagcggc
     5761 cgcaagcaac aacaggaaca ccgccgggag aatgccgaac tgctttttca tgttatcgaa
     5821 tccttaggat tgacgtttag cttgacgcga atccggcgaa caagttccct gctgcgatgc
     5881 ggatgcattg ctc
//
LOCUS       DASUOM010000522         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_65705,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000522 DASUOM010000000
VERSION     DASUOM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65705"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFY27_10870"
     CDS             complement(<1..199)
                     /locus_tag="VFY27_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018881716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766060.1"
                     /translation="MAVILIIDDSPTELHLFQRMLEKNGFNTLVADSGEEGIRQARSR
                     KPDCILMDVVMPGMNGFQATRK"
     gene            complement(210..602)
                     /gene="pilG"
                     /locus_tag="VFY27_10875"
     CDS             complement(210..602)
                     /gene="pilG"
                     /locus_tag="VFY27_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HEX5766061.1"
                     /translation="MSKNPSRSLNGLKILVVDDSKTIRRTAETLLSKEGCQVFTAIDG
                     FDALSKIADHQPDLIFVDIMMPRLDGYQTCSLIKHNKVFRDTPVIMLSSKDGLFDRAR
                     GRIVGSEQYLTKPFTKDELLGAISNQIN"
     gene            920..1885
                     /gene="gshB"
                     /locus_tag="VFY27_10880"
     CDS             920..1885
                     /gene="gshB"
                     /locus_tag="VFY27_10880"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX5766062.1"
                     /translation="MPQSTIHLGVVMDPIETITPKKDSTLAMLLEAQRRGAKIHYLEQ
                     RDLRLIGGEAFGRCRILSVKDDLENWFEYGPSLDVPLADLDVILMRKDPPFDMEYIYT
                     SYILERAEFAGALVVNKPQSLRDMNEKAYTAWFSDCAPLTLVTRSMAEMKEFLAEHHR
                     IVVKPMDGMGGRSIFVVAEGDNNANVIFETLTDNGRRFAMAQVFIPEITDGDKRILLV
                     NGEPIPFCLARIPSKGENRGNLVMGAIGEGRELTDRDRFICSRVGPVLKKRGVTFAGI
                     DVIGDYLTEINVTSPTGIRELDRKYDLNIAGTLFDAIEETLAARR"
     gene            2298..>3014
                     /locus_tag="VFY27_10885"
     CDS             2298..>3014
                     /locus_tag="VFY27_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766063.1"
                     /translation="MTADAFAEIDEAEDLEALAHPGVREEKKKPDRLPPMLFLAALVH
                     GILIIGITFNAMIGDDYADAISLEVTILADPDHTAATPDDADYLAQASQDGGGNTLLQ
                     VRPGALAESNVPINNAGEADGNSLDPAQAHEVAADELLNTVSDTDIAVADSPRVDPKP
                     DEATALALEAGAETTLPLPQDDQVELQIHDDHPRELVTSVDTRESVVAGYLDRWKRKI
                     ETTGIRYFPEQSLLGELTGSP"
BASE COUNT          610 a          913 c          908 g          583 t
ORIGIN      
        1 gttttctcgt ggcctggaag ccgttcattc ccggcatcac cacgtccatc aggatgcagt
       61 cgggcttccg ggaccgggcc tggcgtattc cctcctcgcc gctgtctgcc acgagggtgt
      121 tgaaaccgtt cttctccagc atgcgttgga acaggtgcag ctcggtcggc gaatcgtcaa
      181 tgatgagaat cacagccata caggtttcct caattgatct ggttcgaaat agcacccaga
      241 agctcgtcct tggtgaacgg cttggtgagg tactgttccg accccacgat ccgccccctg
      301 gcgcgatcaa acagcccgtc cttgctcgac agcatgatca cgggagtgtc cctgaacacc
      361 ttgttgtgct tgatcagcga gcaggtctgg tagccatcga gccgcggcat catgatgtcg
      421 acaaagatca ggtcaggctg gtggtcggca atcttggaca gggcgtcgaa accgtcgatt
      481 gccgtgaata cctggcaacc ttccttggac agcaaggtct ccgcggtacg gcgaatcgtt
      541 ttactgtcat ccacgaccag gatcttgagt ccgttcagac ttcgagatgg gttttttgac
      601 actgattttc ctcgcccaat acgtcctcga agattaagga cgtgtcccca agtagcgact
      661 tgtaccatat tgattaagtg gtcgccatga tggctgccac atgccgcaag tttgatgatt
      721 ttattcgctt ttcacagtgt tcgtaaggac gcgatgccgg cttttccgca aaatggctgg
      781 gaataatttt ccgcgtacgg cgaacggcgc gtaatgtgct gtttatcggc cggttcgcgc
      841 ggcagtattt gcgcgtcccg cagcgctgta tagttgttcg ccccaagttt tcttcccgac
      901 aggcaggaca agaagcaaca tgccgcaatc gaccatacat ctcggcgtcg tcatggatcc
      961 gatcgagacg atcacgccga aaaaagacag cactctcgcg atgttgctgg aggcacagcg
     1021 gcggggcgcc aaaattcact atctcgaaca gcgcgacctc cggttgatcg gcggcgaggc
     1081 attcgggcgc tgccggattc tttcggtgaa ggacgacctc gaaaactggt tcgagtatgg
     1141 accttcgctc gacgtgccgc tcgccgatct cgacgtcatc ctgatgcgca aggacccacc
     1201 gttcgacatg gagtacatct ataccagcta tatcctggag cgggccgagt tcgccggggc
     1261 gctggtggtc aacaagcctc aaagcctgcg ggacatgaac gagaaggctt acaccgcctg
     1321 gttcagcgac tgcgccccgc tgaccctcgt cacgcgttcc atggccgaga tgaaggagtt
     1381 cctggcggaa caccatcgca tcgtggtcaa gccgatggac ggcatgggcg ggcgctccat
     1441 cttcgtcgtg gccgaaggcg acaacaacgc caacgtcatc ttcgagaccc tcaccgacaa
     1501 cggccggcga ttcgcaatgg cgcaggtgtt catccccgag atcacggatg gtgacaagcg
     1561 catcctcctc gtcaacggcg aacccattcc tttttgcctg gcgcgcatcc cctcaaaggg
     1621 cgagaaccgc ggcaacctcg tgatgggggc catcggcgaa gggcgcgagt tgaccgaccg
     1681 tgaccgcttc atctgctcgc gcgtcggacc ggtactgaaa aagcgtggcg tcactttcgc
     1741 gggcatagac gtcatcggcg attacctgac ggagatcaac gtcacgagcc cgacgggcat
     1801 tcgcgaactg gacaggaagt acgacctgaa catcgccggg acattgttcg atgctatcga
     1861 ggagacgctt gccgcgcgac gctgactcgc ctgaagtgct gcggccggtc atgaacgttg
     1921 ggagcgaagt cagggctgga gagggccttc atgaacgaat cggcgggagt aggtagcgga
     1981 gccgcgcgag ggggagggag ttgtttcagg aacgccgtga acccatccat gggggcttcg
     2041 cacaaaacat ccatgttttg tgacgatcct gaaacaactc cctccccctc acacgactcc
     2101 gcccggccgt cgtgggcgtt catgaaggcc ctctccagcc ctgacttcgc ctgccgccaa
     2161 tctcagctgt tcacggatcg cccgggttgg acctgcgtct cattggcagc gcggcaggcg
     2221 cgttaaaatg cggcactcct gtgccacaat ccctcgatca ggaccgttga tttacgttac
     2281 gcatggccga tacatcattg acagcagatg ccttcgcgga gatcgacgag gcggaagacc
     2341 tcgaagccct cgcccacccg ggcgtccgcg aagagaagaa gaagccggac cggctgccgc
     2401 cgatgctgtt cctggccgcg ctcgtgcatg gcatcctgat catcggcatc accttcaacg
     2461 cgatgatcgg cgacgactat gccgatgcca tctccctgga ggtcactatc ctcgcggatc
     2521 cggaccacac ggctgcgacg ccggacgatg ccgattacct ggcgcaggcg agccaggacg
     2581 gcggcggcaa caccttgctg caggtgcgtc ccggagcgct ggccgagagc aatgtgccga
     2641 tcaacaacgc gggcgaggcg gatggcaaca gcctggatcc cgcccaggcc cacgaggtgg
     2701 ccgccgacga gttgctcaac acggtgagcg acacggatat tgcggtggca gacagtcccc
     2761 gggtggaccc gaagccggac gaagcgaccg cgctggcgct cgaggccggc gcggagacga
     2821 cgctgccgct gccgcaggac gaccaggtag agttgcagat tcacgacgac catccgcgcg
     2881 agctcgtcac cagtgtggat acgcgagagt cggtggtcgc aggctacctg gaccgatgga
     2941 agcgcaagat cgagacgacc ggcatccgct attttcccga gcagtccctg ctgggcgagc
     3001 tgaccgggag tccc
//
LOCUS       DASUOM010000523         7295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1152980,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000523 DASUOM010000000
VERSION     DASUOM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152980"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..2942
                     /locus_tag="VFY27_10890"
     CDS             267..2942
                     /locus_tag="VFY27_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766064.1"
                     /translation="MATAQDREAPESVISAPTLPAAEENSGAIEEVVVTGRFLSSSQQ
                     LVNERIDDSYVSDVLGEDTISRLGDSTVGAALRRVPGLSLVADKFVYIRGLGERYSAT
                     SLNGAQIPSPDLTRNVIPLDVFPTSIVESLKVQKAWSPDLPANFGGGSVDIRTKGIPD
                     AFVLNFEFGSGYNSLAGDGYTYEGGDDDRFGTDDGTRALSGDVLAAINEYQGSVGVQQ
                     ILTFMRRQDPSATLADAQLVNRDLALELNRNIGAETTDVPLDASARASIGNNWLIGND
                     WELGFLAGGSYQNEWRNTEARARNFNFPEERTDDEDETTRSTTMAGTGTVGLKFTDDH
                     QIETTSLWLRNTDDETAVRDFFNENRQKSDGLGFRNYRIKFEEREMLTHQISGSHFLG
                     TDTRERLPGFLSGLAGVLPDESSVSWFYSESEAQTDIPNEVSVNSQTVTDPVTGAVQS
                     EAVLRDATAADFRFTDLNDEVINHGWSASLPIEFKRNQLVLGGGYNHVRKARTYRQSQ
                     FSIGALAVADPSILRGSLDQVFSDENILDPNNNFVFDRQGSNNESYIAATMTDAAFGS
                     VEWTWNDTWKIATGARWEDYRQVAVSWNPYGYSQANPQVTTDPDVLKRGTFADDEIFP
                     SASVTWMSDLWAETFQLRFGWSETAVRPDLREITEASYVDPVTDDLTFGNPDVEPAAV
                     TNYDVRAEWFFSSGDNLTVSLFRKEIDQPIDFFESPASDTTIAREILNGESAEVQGVE
                     IEGLKELAFLGGFFDTMFVQGNVTLQDSELVSGSRPDAPTNPVRPLGGASEYVANVML
                     GFDSPDSKHTASLIYNVFGERLYVAGRNGAPDAFEQPFHSLDLTYAWYPTDTMTVKLK
                     AQNLLEETVTIERQGIIVFEQDPGINYSLSFSWSP"
     gene            3189..3896
                     /gene="phoB"
                     /locus_tag="VFY27_10895"
     CDS             3189..3896
                     /gene="phoB"
                     /locus_tag="VFY27_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX5766065.1"
                     /translation="MTGIKILIVEDEAPIREMIAFHLARAGYETLEAADCRSARRLLA
                     DERPDLALIDWMLPDMSGLELTRMLKRDNENADLAVIMLTARSDEHDKVSGLEGGADD
                     YVTKPFSPRELIARIQAVLRRSGSADAETLDAGVLELDPASHRVSIKGKEIPLGPTEY
                     RLLKFLMSHPDRVYSRTQLLDRVWGANVYVEERTVDVHVRRLRKALSRGGADRYIQTV
                     RGAGYRFSIRETLQAHG"
     gene            3909..5240
                     /gene="phoR"
                     /locus_tag="VFY27_10900"
     CDS             3909..5240
                     /gene="phoR"
                     /locus_tag="VFY27_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEX5766066.1"
                     /translation="MPSSWRRFLTGLSLLLAGGLMLGWLYGHTLLGFTAACVVALVWQ
                     VQNTLRFEQAARTRNFSHFQFGEGIWPQIYSHFSHLRQRNRLHKRNYQQLLKEVREMT
                     NALPDGGIVLNDASEIVLCNAAARELAGFDEHQDRGQRVDNLLRAPKFIKYLKSGDYG
                     TAVEIPSPIREGAWLLCKIVAYGADQRLLLIRDVTERRRLTTMRREFIANASHELRSP
                     LTVISGYLDTLADDPDIPQHWHKPLGQMREQAGRMSRIIADLLELSRLEGAGVLTGEQ
                     HVDVSALLDSAANLYADLRSNPEIVVQAESQEKLLGSAAELESVIRNLLSNAVRHTPP
                     DGSIQLTWRTGPEGGELIVADNGEGIAAENIPRITERFFRIDQGRSRDDGGVGLGLSI
                     VKHVLIRHDAELAIESKPGEGSRFICRFPPARIIPGSITSAQSNVHSINKF"
     gene            5253..5966
                     /gene="phoU"
                     /locus_tag="VFY27_10905"
     CDS             5253..5966
                     /gene="phoU"
                     /locus_tag="VFY27_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5766067.1"
                     /translation="MQTSDLTDHISRRYNKDLESVRNSVLRMGGLVESQLSKAISAIV
                     SGDSELGLKVAEDDYKVNLLEVEIDEACSRILATRAPTAGDLRLIVAIVKTITDLERV
                     GDEAEKIGFLASKLAIMDRPPDSYRELKNLGNHVLDMLRGAMNAFARLDVPASYAVVR
                     KDELVDEEYDAITRQCITFMMEDPRTIRRSMNITWAARALERIGDHAKNICEYVIYMV
                     EGRDVRHTDLDQAAAQEEV"
     gene            6046..7104
                     /locus_tag="VFY27_10910"
     CDS             6046..7104
                     /locus_tag="VFY27_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5766068.1"
                     /translation="MLRKAAAIVAATASLACGVAFAQAGRDYIYIVGSSTVYPFSTVV
                     AERFGRGSEFRTPKVESTGSGGGLKLFCDGVGVQHPDIANSSRAIKQSEIDACEANGV
                     REIVEVKIGYDGIVLAHGVSADPVSLTREEIFLALAKQVPGEADGDLVANPYRTWQDI
                     NEDLPAADIEVLGPPPTSGTRDAFVELAMEDGCKATPWIAALAESDEGRFMTICHTLR
                     EDGAFVEAGENDNLIVQKLQSNPRALGIFGFSFLDQNTDKVRGAAIDGVEPTFDTIAD
                     GTYPISRPLFFYVKKAHAEVIPGLRDFLMEFTSERAWGEFGYLSDRGLIPLPEEERDE
                     VASEVRSLTPLADAGRFE"
BASE COUNT         1491 a         2203 c         2272 g         1329 t
ORIGIN      
        1 gattccgtac gcgacctcgc aggttgacgg agtcgcaccg ggagcgggca gcgacccggc
       61 accggagctg tacctcggag cagtgagcct gggcgagacg gattggactg cgggctggac
      121 ttttggtctg cacgccgaca gccaggccca gccgctgtgg ttcgaggctc tttgactttg
      181 acatgagcgc tatgagtagc acaacaacaa ccgtatccag ctcctttgcg tgggcagccg
      241 cactggcatt gctgtcgtgt ccagcaatgg caacggccca ggatcgggaa gctcctgaat
      301 ccgtcatcag cgcgccgacc ctgcccgccg cggaggagaa cagcggcgcg atagaagaag
      361 tcgtcgtgac cggccggttc ctgagctcga gccagcagct tgtgaacgag cgcatcgacg
      421 attcctacgt cagtgacgtg ctgggcgagg atacgatcag ccggctcggc gacagcactg
      481 tcggcgcggc cctccgccgg gtccccggtc tttcgctggt cgcggacaag tttgtctata
      541 tccggggcct cggcgaaaga tattccgcga cgtcattgaa cggtgcacag ataccttcgc
      601 ccgacctgac gcgaaacgtc ataccgctgg acgtgttccc gacctcgatc gtcgagtctc
      661 tcaaggtcca gaaagcgtgg tcgcccgacc tgccggccaa cttcggcggc gggagcgtcg
      721 acatacgcac caagggcata ccggatgcat tcgtgctgaa cttcgaattc ggcagtggct
      781 acaactccct ggcgggagac ggatatacct acgagggcgg cgacgacgac agatttggaa
      841 ccgatgacgg cacgcgcgcc ttgtccggtg acgtgctggc cgcgatcaac gagtaccagg
      901 gcagtgtcgg cgtacagcaa atcctcacgt tcatgcgccg gcaagaccct tcggccacgc
      961 tggcggacgc ccagctcgtc aaccgtgacc tcgcgctcga actgaaccgc aacatcggcg
     1021 ccgagaccac ggatgtgccg ctggatgcca gcgcgcgtgc cagcatcggc aacaactggc
     1081 tgatcggcaa cgactgggaa ctcgggttcc tcgccggcgg ctcctaccag aacgagtggc
     1141 gcaacacgga ggcgagggcc aggaacttca acttcccgga agaacgcacg gacgatgagg
     1201 acgagacgac ccgatcgacc acgatggcgg gcacgggtac cgtcggcctg aagttcacgg
     1261 acgatcacca gatcgagacc acgtcgctgt ggttgcgcaa caccgacgac gagacggccg
     1321 tccgggactt cttcaacgag aaccggcaga agtcggacgg gctcggtttc aggaattacc
     1381 ggatcaagtt cgaggaacgt gaaatgctga cccaccagat cagtggcagc cactttctcg
     1441 gcacagacac gagggaaaga ctgcccgggt tcctgtcggg cttagcaggc gtccttccgg
     1501 acgaatccag cgtatcctgg ttttactccg agtcggaggc ccagacggat attccgaacg
     1561 aagtcagcgt caactcgcag accgtcacgg acccggttac gggggccgtg cagagcgagg
     1621 ccgtcctgcg cgatgcgacc gcggcggact ttcgcttcac cgacctcaat gacgaggtca
     1681 tcaatcatgg gtggtcagcc tcgctgccga tcgagttcaa gagaaaccag ctcgtcctgg
     1741 gcggcggata caaccacgtg cgcaaggccc ggacctaccg gcagtcgcag ttcagcatcg
     1801 gtgcgctggc ggttgccgat ccgtcgatcc tgcgcggttc gctcgaccag gttttctcgg
     1861 acgagaacat actggacccg aacaacaact tcgtcttcga tcggcagggt tcgaacaacg
     1921 agagctacat tgctgcaacc atgaccgatg cggctttcgg ctcggtcgaa tggacgtgga
     1981 acgacacctg gaaaatagcc acgggcgcgc gctgggagga ttaccgccag gttgccgtct
     2041 catggaatcc ctacggttat tcacaggcga atccgcaggt cacgacagat ccggatgtcc
     2101 ttaagcgggg aactttcgcc gacgacgaga tctttccgtc ggcgtccgtg acctggatgt
     2161 ccgatctctg ggcagaaacc ttccagctgc gcttcggctg gagcgaaacc gccgtacggc
     2221 ccgatcttcg ggagatcacc gaggccagtt acgtcgatcc ggttaccgac gacctgacct
     2281 tcggcaatcc ggatgtcgag cccgccgcag taacgaacta cgacgttcgc gccgaatggt
     2341 tcttctcgag cggagacaac ctgaccgtct ccctcttccg caaggaaatc gaccagccga
     2401 tcgacttctt cgaatcaccg gcaagcgata cgaccattgc ccgcgagatc ctcaatggag
     2461 agtctgcgga ggtccagggg gtcgagatcg aggggttgaa ggaactcgcg ttcctcggcg
     2521 gtttcttcga cacgatgttc gtgcagggca acgtcacgct gcaggactcg gagctggtat
     2581 cgggctcgcg tcccgatgcg ccgaccaacc ccgtgcggcc actgggcggc gcgtccgagt
     2641 atgtcgcgaa cgtgatgctc gggttcgact cgccggactc gaagcacacg gcctccctga
     2701 tttacaacgt cttcggcgag cgcctctacg tcgcgggccg gaacggcgcg ccggacgcct
     2761 tcgaacaacc cttccattcg ctcgacctga cgtacgcctg gtacccgacc gatactatga
     2821 ccgtcaagct caaggcacag aacctcctgg aagagacggt tacgatcgag cggcagggca
     2881 tcattgtttt cgagcaggac cccgggatca attactcgct cagtttctcc tggtcgcctt
     2941 aaggggtcga accgattttt ccggagcgtt aagggaccgc gagcgttaag gggtacccgg
     3001 aaaaatcggt tcgacccttg gtactccctg caaactgtcg ctttgaggct ccgaggtaac
     3061 tcccggagct tttctttttc tgaccccacc cctcgacacg agggcaacgg ccgtttcggt
     3121 cgtacaatga cggctcaccg taacagatct gtaacaaaag cttgctgtaa tctgccccgg
     3181 aacagaccat gaccggaatt aaaatactga tagttgaaga cgaggcgccg attcgcgaga
     3241 tgattgcctt ccatctcgct cgcgcgggct acgagacgct cgaggcggcg gactgccgca
     3301 gcgcaaggcg gctcctggcg gacgagcgtc ccgacctcgc gctgatcgac tggatgttgc
     3361 cggacatgag cggtctcgag cttacccgca tgctgaaacg cgacaacgag aacgcagatc
     3421 ttgcggtcat catgctgacg gctcgcagcg acgaacacga caaggtctcg gggctggaag
     3481 gcggcgcaga cgactatgtc accaagccgt tctctccacg cgagctcatc gcgcggatcc
     3541 aggccgttct gcgcagatcg ggatctgccg atgcagagac gctcgatgca ggtgtgctgg
     3601 agttggaccc cgcaagccat cgcgtgagca tcaagggcaa ggagattccg ctcgggccga
     3661 ccgaataccg attgctcaag ttcctgatgt cgcatccgga tcgcgtatac agccgcaccc
     3721 agttgctgga ccgggtgtgg ggcgctaacg tctatgtcga agagcgaacc gtcgacgtgc
     3781 acgtgcggcg cttgcgcaaa gcgttgtccc ggggcggcgc tgaccggtat atacagaccg
     3841 taagaggcgc cggttaccgc ttctcgattc gagaaacgtt acaggcgcac gggtaaggcg
     3901 gcgcgttcat gccaagtagc tggcggcggt ttctcaccgg gctttccctg cttctcgccg
     3961 gcgggctcat gctggggtgg ctgtatgggc acacgctcct gggcttcacc gcggcttgcg
     4021 tcgttgccct cgtgtggcag gtacagaaca cgctgcgctt cgagcaggcg gcgcgcaccc
     4081 gcaacttcag ccatttccag ttcggcgaag gcatctggcc gcagatttac tcccacttca
     4141 gccacctgag gcagcgcaac cgcctgcaca agcgtaacta ccagcagttg ctcaaggaag
     4201 tccgggaaat gacgaatgcg ctgcccgacg gtggaatcgt cctcaacgat gcgagtgaaa
     4261 tcgtgttgtg caatgcggca gcccgggaac tggcgggctt cgatgagcac caggatcgcg
     4321 ggcagcgggt cgacaacctc ctgcgtgcgc cgaaattcat caagtacctg aagtccggtg
     4381 actacggcac ggcagtcgag atcccgtcgc cgatccggga gggtgcatgg ttgctttgca
     4441 agatcgttgc gtatggcgcc gatcaacggc ttctcctgat tcgtgacgtt acggaacgcc
     4501 ggcggctgac gacgatgcgg cgcgaattta tcgccaatgc gtcccatgaa ctgagatcgc
     4561 cgttgacggt gatcagtggg tacctggaca cacttgctga cgatccggat attccgcaac
     4621 actggcacaa gccgctggga cagatgcgtg aacaggccgg tcgaatgagc cggatcatcg
     4681 cggacctgct cgagttgtcg cgcctggagg gagcgggtgt gctcaccggc gaacagcacg
     4741 tcgacgtgtc cgcgttgctc gattctgcgg caaacctgta cgcagacctg cggtctaatc
     4801 cggagatcgt ggttcaggcg gagtcgcagg agaagctgct gggcagcgcc gccgagcttg
     4861 agtcggtcat ccggaacctg ttgtcgaatg ccgtgaggca cacgccgccc gacggttcga
     4921 ttcaacttac ctggcgcacc ggacccgagg gcggcgagct gatcgttgca gacaatggcg
     4981 aaggaatcgc cgcggaaaat attccgcgca taacggaacg cttcttccgc atcgaccagg
     5041 gccggagccg ggacgacggc ggagtcggtc tcgggctttc gatcgtcaag cacgtgctca
     5101 tccgtcacga cgccgaactg gctatcgaaa gcaagcctgg tgaaggcagc cgcttcatct
     5161 gccgctttcc gcccgcgcga atcattccgg gaagcattac atcagcgcag tcaaacgttc
     5221 actcaataaa caagttctga ggcctcaata tcatgcaaac ctccgatctg accgaccata
     5281 tctccaggcg atacaacaag gacctcgaga gcgtcaggaa cagcgtcctg cgaatggggg
     5341 ggctcgtcga gtcccagttg tccaaagcga tctccgcgat cgtcagcggt gacagcgaac
     5401 ttgggctcaa ggttgccgaa gacgattaca aggtcaacct gctcgaagtc gagattgacg
     5461 aagcatgcag ccggattctc gcgacacgcg cgccgacggc cggggacctg cggctgatcg
     5521 tcgccatcgt gaaaaccata accgatctgg aacgcgtagg ggacgaagcc gagaagatcg
     5581 gcttcctggc atcgaagctc gcaatcatgg atcgcccgcc cgactcctat cgcgagttga
     5641 agaacctggg aaaccatgtt ctggacatgt tacgcggcgc gatgaatgcc tttgcccggc
     5701 tggacgttcc cgcgtcttat gcggtcgtcc gcaaggacga actcgtcgac gaggaatatg
     5761 acgcaatcac gcgccagtgc ataaccttca tgatggaaga tccgcgcacg ataaggcggt
     5821 cgatgaacat cacctgggcc gccagggcgc tcgagcgcat cggggatcac gcgaagaaca
     5881 tctgcgagta cgtcatctac atggtcgagg ggagggatgt gcgccacacg gacctggacc
     5941 aggccgcggc ccaggaagaa gtttgaggag cccgccccgc atgcaacaaa tccgtaacat
     6001 tccgtcgcta agctggtggg caaagcgaca tacggagaga gtgaaatgtt gaggaaagct
     6061 gccgccatcg tggcggccac ggcgtcgctc gcgtgtggcg tcgcctttgc gcaggcgggg
     6121 cgggactaca tatatatagt cgggtcttcc acggtttatc cgttctcgac cgtcgttgcc
     6181 gagcgatttg gccggggtag cgagttcagg acgccgaaag tggagtcgac cggctccggt
     6241 ggcggcctga agctgttctg cgatggcgtc ggcgtgcagc atccggacat cgccaattcg
     6301 tcccgcgcaa tcaagcagtc cgaaatagac gcctgcgagg cgaatggcgt gcgagagatc
     6361 gtcgaggtca agatcggtta cgacgggatc gtgcttgctc atggcgtgtc tgcggacccg
     6421 gtgagcctga cacgggagga aatattcctg gcactggcca agcaggtgcc cggggaagcg
     6481 gacggcgatc tcgtcgcaaa tccttaccgg acctggcagg acatcaacga ggatctgcct
     6541 gcagcagata tcgaggtgct cggacctccg ccgacctccg gcacccggga tgcctttgtc
     6601 gagcttgcca tggaggatgg atgcaaggcg actccgtgga tcgctgcgct cgccgaatcg
     6661 gatgaaggcc gtttcatgac gatctgccat acacttcggg aggatggtgc gttcgtcgaa
     6721 gccggtgaga acgacaatct cattgtacag aagctgcagt caaacccccg cgcgctgggc
     6781 atattcggct tcagctttct cgaccagaac acggacaagg tacgaggggc cgccatcgac
     6841 ggcgttgagc cgaccttcga cacgatcgcg gacggaacct acccgatatc gcggccgctc
     6901 ttcttctacg tcaagaaagc gcacgccgag gtcattcccg gcttgcggga tttcctcatg
     6961 gagttcacca gcgagagagc gtggggagag ttcggttacc tgtcggaccg cggcctgatc
     7021 cccctgcccg aagaagagcg ggacgaagtc gcatccgagg tgcgcagcct gacgccgctt
     7081 gccgatgcag ggcggttcga gtagcggcgc cggaaccagg aaaaccgttt ccacctcatg
     7141 cagctcgcaa ccctcgctct cgtcctcacc gccttgtcgg ttgcggcatt ctggctcggg
     7201 cgacatcggg cgctggaggc ggccggtggc ccgcaccaga aagtgcgcct gcattcactg
     7261 ccgggacact acggctacta tgctgcgatc tggtt
//
LOCUS       DASUOM010000524         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_549512,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000524 DASUOM010000000
VERSION     DASUOM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549512"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /gene="iolC"
                     /locus_tag="VFY27_10915"
     CDS             complement(<1..1911)
                     /gene="iolC"
                     /locus_tag="VFY27_10915"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEX5766069.1"
                     /translation="MSSRSSGVSKHDVICLGRAAVDLYGQQSGGRLEDMQSFAKYLGG
                     SSGNLAAGLARLGIRSAMLTRVGNEQMGRFVREQLAREGVDVTRVRTDPDRLTALVVL
                     GIMSADDIPHIFFRERCADMGLCEEDIDEAFIASARMLAITGTHLSTTSTRNAVQKAI
                     RAANEGGTKVVLDIDYRPVLWGLANAGEGASRFAESREITEILQGFLPDCDLIVGTEE
                     EIAIAGGETDMVKALNTIRSLSDAIIVLKRGASGCSIFDSGHIDSLDDGIAVSGAEID
                     VFNTVGAGDAFLSGFLCGVLSDLSLEDSGALGNACGALVVSRHGCTPAMPSRIELDAW
                     LSRRNPPRQPQNDAELAHLHRTTTWRDDPRPLCALAFDHRSQFEQLAAQHGRPFDAIE
                     EFKELIAEVLLEAVDESDSSVRYGAIVDHRYGGKALARLAGAAIWTGSPIEIPGSRPL
                     EFEPQFATASHLCTWPAHQVVKCLMFYHPDDDPALRHEQEQRIRALYADLLALDRRLI
                     LEVICPTTEHPVTAETLPRAMRRLYNLGVRPDWWKLEARSAAGWEAVSSVIRKNDPHC
                     KGVVLLGLGADEKTLANGLTVAAGYDICRGFAVGRSIFADAASRWFAGEIDGSAAKDA
                     IKQRYLDMIDVWC"
     gene            complement(1895..2701)
                     /locus_tag="VFY27_10920"
     CDS             complement(1895..2701)
                     /locus_tag="VFY27_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX5766070.1"
                     /translation="MNDAQQHVIRHHGGPPGRGDLIAEFSGLRVSSILLDAGESIAKE
                     TDTETAWLLMEGKVRGQAGDTKFELERHSLFDESASCLHVSAETAVTLEAMTACELTV
                     YQCPNRKHFPARVFTPADVPNEHRGKGQVAERCLRFVRTIFDGSNAPENAELVLGEVV
                     TLPGGWSSYPPHHHRQPEIYHYRFTEPQGYGHAELGDEVFKVTDRDTVCIPPGLDHAQ
                     CAAPGYGMYYSWVIRHLPNDPYTIPDFTEEHAWTMDKDAVYWWPADVEQE"
     gene            complement(2732..>3097)
                     /locus_tag="VFY27_10925"
     CDS             complement(2732..>3097)
                     /locus_tag="VFY27_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5766071.1"
                     /translation="RVATGYKCGLQFEIFGTRGSIAFDQERMNELRVYDGEQRPARAG
                     FKTILSGPAHPDYGNFCPAPGHGLGINDLKVIEVRNLLRAIQHGEPFQPDFEEGLRVQ
                     EVMEAIERSSAERAWVELG"
BASE COUNT          544 a          987 c          958 g          608 t
ORIGIN      
        1 gcaccagacg tcgatcatat cgaggtagcg ctgcttgata gcgtctttcg cggcagaacc
       61 gtcgatctcg ccggcgaacc atcgggaagc ggcgtcggca aagatgctgc ggccgactgc
      121 gaagccgcgg cagatgtcgt accctgcggc aacggtcagg ccattggcaa gtgtcttttc
      181 gtccgcgccg agaccgagaa gcacgacacc cttgcagtgc gggtcattct tccggatgac
      241 cgaggaaacg gcttcccagc ccgcggccga tcgtgcttcc agtttccacc agtccggccg
      301 cacgccgagg ttgtagagcc ggcgcatcgc gcgcggcagc gtctccgccg tcacaggatg
      361 ttccgtggtt gggcagatca cttccagtat caggcggcgg tcgagcgcca gtaggtcggc
      421 gtagagcgcg cgaatacgct gttcctgttc atgcctcaat gccggatcgt catccgggtg
      481 gtagaacatc aggcacttga cgacctggtg ggccggccac gtgcagaggt gcgatgccgt
      541 cgcaaattgc ggctcgaatt ccagcggcct cgagcctgga atctcgatcg ggctgccggt
      601 ccagatggcg gcgcccgcga gcctggccag ggctttgccg ccatagcggt ggtcgacaat
      661 ggcgccgtaa cgcactgacg aatcgctttc atcgactgcc tccagcagga cctctgcgat
      721 gagttccttg aactcttcga tagcgtcgaa gggacgcccg tgctgagcgg caagctgttc
      781 gaactggctg cgatgatcga aagccagtgc gcacagcggt cgggggtcgt cgcgccaggt
      841 ggtcgtgcgg tgcaggtgag cgagttctgc gtcgttttgc ggctggcgcg gcgggttccg
      901 gcgcgacagc catgcgtcca gttcgatgcg gctcggcatt gccggagtgc agccgtggcg
      961 ggagacgacc agggcgccgc acgcgttgcc gagcgcgccg ctgtcctcga gcgacaggtc
     1021 gctaagtact ccgcagagga agccggacag aaacgcatca ccggcgccca ccgtattgaa
     1081 aacgtcgatc tccgctccac tcaccgcgat gccgtcgtcg agagaatcga tgtgtccgga
     1141 gtcgaagatg gaacagccgg atgcgccgcg cttcagcacg atgatggcgt cgctcaagct
     1201 ccggatggtg ttgagcgctt tcaccatgtc ggtttcaccg ccggcgattg cgatttcctc
     1261 ttccgtgccg acgatcaggt cgcagtccgg caggaagccc tgcaggattt ccgtgatctc
     1321 acgcgattcc gcgaagcggc tcgcgccttc accggcgttc gcaagccccc acaagacggg
     1381 cctgtaatcg atgtccagca cgactttcgt gcccccctcg ttcgccgccc ggatggcttt
     1441 ctgcaccgca ttgcgagtgg atgtcgtcga aaggtgcgtt ccggtgatcg cgagcatccg
     1501 ggcagaggcg atgaatgctt cgtcgatgtc ctcttcgcaa aggcccatgt cggcacagcg
     1561 ctcgcgaaag aaaatatgcg gtatgtcatc cgcgctcatt attcccagca cgaccagcgc
     1621 ggtcaggcga tccgggtccg ttcttacccg cgttacatca actccctccc gcgccagttg
     1681 ctcacggacg aaacgtccca tctgttcatt gccgacgcgg gtaagcatgg cgctgcgaat
     1741 accgaggcgg gcaagccccg cggcgagatt tcccgacgaa ccgccgagat atttagcaaa
     1801 actctgcatg tcctccaggc ggccacccga ctgctggcca tagaggtcca ccgccgcgcg
     1861 cccgaggcaa atgacgtcgt gcttactgac gccgctactc ctgctcgaca tcggccggcc
     1921 accagtacac cgcatccttg tccatcgtcc aggcatgctc ttccgtgaaa tcgggaatcg
     1981 tgtaggggtc gttcggcagg tggcggatca cccaggagta gtacatgcca tagccgggag
     2041 cagcgcattg cgcgtggtcg agcccgggcg gaatgcagac cgtatcgcgg tcggtgacct
     2101 tgaagacctc gtcgcccagc tcggcgtgcc cgtagccctg cggttccgta aagcgatagt
     2161 gatagatttc cggctgccgg tggtgatgcg gcgggtagct ggaccatcct cccggcagcg
     2221 tcacgacttc gcccagaaca agttccgcgt tttccggtgc gttgctgccg tcgaaaatcg
     2281 tgcgcacgaa gcgcaggcaa cgctccgcga cctggccctt gccgcggtgt tcgttcggca
     2341 cgtcggcagg cgtgaagacg cgtgcgggaa aatgcttccg gttcgggcac tggtataccg
     2401 tcagttcaca ggcggtcatc gcctccagcg tcaccgctgt ttctgcagac acgtgcaggc
     2461 aactcgccga ttcgtcgaac agggagtgcc gttccagttc gaactttgta tctcctgcct
     2521 gccccctgac cttgccttcc atcaggagcc acgccgtttc cgtatccgtt tccttagcga
     2581 tgctctcccc ggcatcgagc aggatactgc tgacacgaag tccggaaaac tcggcaatca
     2641 gatcgccgcg tcccggcgga cctccgtggt gacggatgac atgttgttgc gcatcgttca
     2701 tcacgtaact cccgttgagt ctgtcacagg gtcagcccag ctcgacccat gccctctcgg
     2761 cgctcgatcg ctcgatcgcc tccatgacct cctgcacgcg cagtccctcc tcgaaatcag
     2821 gctggaaggg ttcgccgtgc tggattgcgc gaaggaggtt gcggacctcg atgaccttga
     2881 gatcgtttat tccgaggcca tgccctgggg cagggcagaa gttgccgtag tcgggatgcg
     2941 cgggcccgct caggatcgtc ttgaatcccg ctcgtgccgg ccgttgctcg ccgtcataaa
     3001 cgcgcaattc gttcatgcgt tcctggtcga aagcaatgct gccccgggtg ccgaaaatct
     3061 cgaactgcaa gccgcacttg tagccggtgg caacccg
//
LOCUS       DASUOM010000525         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1514747,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000525 DASUOM010000000
VERSION     DASUOM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514747"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFY27_10930"
     CDS             <1..747
                     /locus_tag="VFY27_10930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5766072.1"
                     /translation="EWPADVGLDKAGITLEDLLTMRSGLRTTSNRNYATWVLSDNWVV
                     SALRQPLEATPGQEMRYSTGNTHLLSAILTTASGRSTLEFAREALAEPLGFELAPWPR
                     DPQGIYFGGNDMRLTPRQMLAFGELYLNGGRRGEQQIVPASWVEASLQPRSKSPHGDA
                     RYYGYGWWICALVGYQVPHAWGHGGQFIMLVPDLDLVIVTTSSPNAGEGAHEHANQVY
                     QWLQQIIYLVANAPSPPPANERETVAHQRH"
     gene            complement(675..1001)
                     /locus_tag="VFY27_10935"
     CDS             complement(675..1001)
                     /locus_tag="VFY27_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5766073.1"
                     /translation="MNPNNEKMRRRIPWNVWLVLGGCGSAVLAAGFPERGIGLLGVSL
                     AFGLTLLTMAGAIGHISGCHINRRSLAGGGAGLRGVARSRTQWRWCATVSRSLAGGGD
                     GAFATR"
     gene            complement(985..1695)
                     /locus_tag="VFY27_10940"
     CDS             complement(985..1695)
                     /locus_tag="VFY27_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766074.1"
                     /translation="MGKVSHQATEGYESPSENDIHHAVGAGESRRPTVAVLDDDEAIL
                     RSIGRALAAQGFDARLFSSPALLLAEIASVAPSCLIADLAMPGMTGLDVQRALAMQGL
                     KCPVIFVTGHGDVRTSVQAMRGGAVDFLTKPFELYELLDALERAISKSRHFREIDDRL
                     ASVQRRVESLTPREREVFKQVIDGLLNKQIAANLGISEKTVKVHRGRVMRKMSVRSVA
                     QLVRAAGQVERLQNEPEQ"
     gene            complement(1718..>2117)
                     /locus_tag="VFY27_10945"
     CDS             complement(1718..>2117)
                     /locus_tag="VFY27_10945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5766075.1"
                     /translation="GFKRVNDEIGHLGGDRLLQQVGERLTSCIRGSDTACRFGGDEFV
                     VLLADIGLGRRPRTKAKDKIISQLTMPYVIDGTTVRIGASIGMATYPTDGKNYDELMR
                     FADFTMYRNKVAPSRDEFFPKPSRRCAYGA"
BASE COUNT          416 a          670 c          620 g          411 t
ORIGIN      
        1 gaatggccgg ctgacgttgg tcttgacaag gccggcatca cgctggagga cttgctgacg
       61 atgcgctcgg gattgcgaac tacgagtaac cgcaactacg ccacctgggt cctcagtgac
      121 aactgggtag tgtccgcgtt gcgtcagccg ctggaggcga cacccggcca ggagatgcgg
      181 tacagcacgg gaaacaccca cctgctttct gcaattttga cgactgcgag cgggcgcagc
      241 actttggagt ttgcccgcga ggcgctggct gagccgctgg gcttcgagct cgcgccctgg
      301 ccccgggatc cacagggcat ctacttcggc ggcaacgaca tgagactgac gccccgacaa
      361 atgctcgctt tcggcgagct gtatctgaac gggggccgtc ggggcgagca acagatcgtg
      421 ccagcctcat gggtcgaggc ttccctgcag ccgcgctcga aatcaccgca cggggacgcg
      481 cggtattatg gttatggctg gtggatctgc gcgctggtcg gataccaggt tccacatgcg
      541 tggggccacg gcggccagtt catcatgctg gtcccggatc tcgatctcgt gatcgtgacg
      601 acgtcctcac cgaatgccgg tgagggagct cacgagcatg cgaaccaggt ctatcagtgg
      661 ctacaacaga taatctacct ggtcgcgaat gcaccatcgc cgccgccggc caatgagcgc
      721 gagaccgtgg cacaccagcg ccattgagtc ctgctccgcg ccaccccacg cagaccagcg
      781 ccgccgccag cgagtgaccg ccggttgatg tgacatccgg agatgtgccc gatggcacct
      841 gccatcgtga gcaaggtcaa gccgaacgcg agcgaaaccc ccaggagccc gatgccgcgc
      901 tccgggaatc cggcagcgag gacagcgcta ccgcatccgc ccaaaaccag ccagacgttc
      961 caaggaattc ggcgccgcat tttttcattg ttcgggttca ttctgcaggc gttctacttg
     1021 accggctgcg cgcaccaatt gcgccacgga ccgcacggac atcttccgca tcactctgcc
     1081 gcgatgcact tttaccgtct tctcggaaat gccgagattc gccgcaattt gcttgttgag
     1141 cagaccgtcg atgacctgtt tgaacacttc ccgctcacgg ggcgtcaggg actcgacccg
     1201 gcgctgcacc gaggcaagcc gatcgtcgat ttcacggaag tgccgactct tcgagatggc
     1261 gcgttccagg gcatccagca gctcgtacaa ctcaaatggc ttggtaagga agtccactgc
     1321 cccgccgcgc atggcctgca cggatgtccg gacgtccccg tgtccggtca cgaagattac
     1381 cggacacttc aagccctgca tcgcgagagc tcgctgcaca tcgaggccgg tcataccggg
     1441 catggcgagg tctgcgatca ggcacgaggg tgcgacagac gcgatttcgg ccagcagcaa
     1501 cgctggtgaa gaaaacaacc tcgcatcgaa tccctgagcg gcgagtgcac gtccaatact
     1561 acggagtata gcttcgtcgt cgtccagaac ggcgacggtt ggccgccgcg actctccagc
     1621 gcctacagca tggtggatgt cattttcact cgggctttca tatccttcgg tcgcctggtg
     1681 actgacctta cccatagcgc atgccggata aacgtctcta tgcaccataa gcacaccggc
     1741 ggctcggctt cggaaagaac tcgtcacggg atggcgcaac cttattgcgg tacatggtga
     1801 agtcagcaaa tcgcatcagt tcatcgtaat ttttcccgtc ggttggatag gtggccatac
     1861 cgatgcttgc cccgatccgg actgtcgtgc catcgatgac gtacggcatt gtcagctgac
     1921 tgataatctt atctttcgct ttggtcctgg ggcgccgccc cagtccgatg tccgccagca
     1981 ggactacaaa ttcgtcgcct ccgaagcggc aggccgtgtc ggaaccgcgg atacaagacg
     2041 tcagtcgctc ccctacctgt tgcagcaacc ggtcgcctcc cagatgcccg atttcatcat
     2101 tgacgcgttt gaacccg
//
LOCUS       DASUOM010000526         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1279944,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000526 DASUOM010000000
VERSION     DASUOM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279944"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFY27_10950"
     CDS             <1..356
                     /locus_tag="VFY27_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5766076.1"
                     /translation="VTVVDGRIDVMEAMRIMKRVGATSLIVERRHENDEYGMVLFSDI
                     AKQVIAKDRAPERVNAFEIMAKPVISVRPEMSIRHCARLFERFGISHAPVIEDDKVIG
                     IVSYYLLVLGSLDLD"
     gene            367..1617
                     /locus_tag="VFY27_10955"
     CDS             367..1617
                     /locus_tag="VFY27_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5766077.1"
                     /translation="MTLLVTAITVVLVVSFLCSVFEATLLSVSPAQVGTLSRKHRRAG
                     QLMSDYKRNIDRPIAAILILNTAAHTVGAAVAGASYTTLYGTQSLWVFTIVFTISVLL
                     FTEIIPKTLGVTYAGALARPAALGIWVMTSVLRPLVAVSERISRALRGDSETPVTSVE
                     EIRLLAALGRTEGAVGVRTANMIVGATHLRQLTASDVMLPRQQVVLLSEEMSREEIVT
                     TARQSGHSRFPFSPGNDLDQVTGIVIVKELLYWLHDHPGESIDWPTLVREPIVVPETM
                     SLLALLRTFQDARRHMAIIVDEYGDVEGIVTLEDVLEEIVGEILDESDRPIEDIWPQP
                     DGSLHVRATVDLRKICTKLDLPWEPEDEVTTVGGLVTELLGRLPLRGDAVVWNGCRVE
                     ILAAGDRRAELIAVSRIDRNAEDH"
     gene            complement(1637..2245)
                     /locus_tag="VFY27_10960"
     CDS             complement(1637..2245)
                     /locus_tag="VFY27_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5766078.1"
                     /translation="MSEYLPAELSQTHLLLLIVAVLLLFLLLFVHRRRRKDSRSLSKV
                     FAAIAYERIDGLVVPNGDDGEIQIDHLLLTAKGLLVIDVKDVVGTVFGSDKMQDWTVI
                     NEERRFTFANPQPALYDRIAAIRSIVRQVPVTGRLLFLDGAAFTKGIPRLVVDCDELL
                     QEFAEKDKTSAKMKVEAFKPHWEHIRSKAVPSEAGKLIRYRA"
BASE COUNT          467 a          713 c          740 g          463 t
ORIGIN      
        1 acgtcactgt cgtcgacggg cgcatcgacg tcatggaggc gatgcgcata atgaagcgag
       61 tcggcgctac cagcctcatc gtcgagagac gtcatgaaaa cgacgagtac ggcatggtgc
      121 tgttctcgga tatcgccaag caggtgattg ccaaggaccg cgcaccagaa cgcgtcaacg
      181 cgttcgagat catggccaag ccggtgattt cggtaaggcc ggagatgagc atccggcatt
      241 gcgcccggct cttcgagcgc ttcggcatca gccacgcgcc ggtcatcgaa gatgacaagg
      301 tcatcgggat cgtaagctac tacctcctgg tgctcggttc gctggacctc gactagtgga
      361 gaaaccatga ccctcctcgt caccgcaatt acggtggtac tggtagtttc tttcctttgt
      421 tctgtcttcg aggcgacgct cctgtccgtc agcccggcgc aggtgggcac gctgtcgcga
      481 aagcatcgcc gcgccggcca gctgatgtcc gactacaagc gcaacatcga caggccgatc
      541 gccgcgatac tcatcctgaa taccgcggct cacacggtcg gcgccgcagt tgccggcgcc
      601 agctacacca ccttgtacgg cacccagtca ctctgggtat ttacgatagt gttcacgatt
      661 tcggtgcttc tgttcaccga gatcattccg aagactcttg gcgtcacgta cgccggcgcg
      721 ctcgcgaggc ctgctgcgct cggcatctgg gtgatgacct ccgtcctgcg accgctcgtt
      781 gccgtcagcg agcgcatctc gcgtgcattg cgcggcgatt ccgagacgcc ggtgacgtcg
      841 gtggaagaga tccgccttct ggcggcgctc ggacggacag aaggggctgt cggcgtgcgt
      901 acagccaaca tgattgtcgg cgcaacgcac ctgcggcagt tgacggcatc cgacgtgatg
      961 ctgccgcgcc agcaggtcgt gttgttgtcg gaggaaatga gccgcgagga aatcgtaacg
     1021 acggcacgcc agtccgggca cagccgcttt ccattcagcc ccgggaacga cctcgaccag
     1081 gttaccggta tcgttatcgt caaggaactc ctttactggc tgcacgacca cccgggcgaa
     1141 tcgatagact ggccgacgct cgtccgcgaa cccattgtcg tgccggagac gatgtccttg
     1201 ctggcgctcc tgcgcacatt ccaggatgcg cggcgacaca tggcgatcat cgtcgacgaa
     1261 tatggcgacg tcgaaggcat cgtcacgctg gaggacgtgc tggaagagat tgtcggtgag
     1321 atcctggatg agagcgacag gcctatcgag gacatctggc ctcagcctga cggctcgtta
     1381 cacgtgcgcg ccacggtcga tttgcgcaag atttgcacca agctcgactt gccgtgggag
     1441 ccggaggatg aagtaaccac ggttgggggg ctcgtcaccg agctccttgg ccgcttgccc
     1501 ctaaggggcg acgcagtggt gtggaacggt tgccgggtgg agatccttgc tgccggggat
     1561 cggcgggccg aactgattgc ggtgagccgg atcgaccgta acgcggagga tcactgacca
     1621 acccgctccc ggtgcctcag gccctgtagc ggatcagctt acccgcttcg gacggcacag
     1681 ccttgctgcg tatgtgttcc cagtgaggct tgaaggcttc caccttcatc ttcgcgcttg
     1741 tcttgtcctt ctctgcaaat tcctgcagca actcatcgca atcgacaacc agcctcggga
     1801 tgcccttcgt aaatgccgcg ccatcgagga acagcaggcg gccggtcacc ggcacctgac
     1861 ggacgattga gcggatcgcg gcgatgcggt cgtacagcgc cggttgcgga ttggcgaagg
     1921 taaagcggcg ctcttcgttg atgacggtcc agtcctgcat cttgtcgctg ccgaagacag
     1981 ttccgacgac atcctttaca tcgatgacaa ggagcccttt cgccgtgagc agcagatgat
     2041 cgatttgtat ctcgccgtcg tcgccgttcg gcacgacaag accatctatg cgttcatagg
     2101 cgatcgcggc gaaaactttg gaaagggagc ggctgtcctt gcgccggcga cggtgcacga
     2161 acaacaacag gaagagcaac aggaccgcaa cgatcagcag cagcaaatga gtttgagaca
     2221 gctccgcggg aaggtattca gacatcgttc ggatatccac gcgtggacac tcttatttat
     2281 cgcccgcgga cgcgcgtgcg tcaaggccga agcgctcttc cagggccgtt acaaacgccg
     2341 gaagatcggg cgagatcgcg acggaacggg aggggcgatc gag
//
LOCUS       DASUOM010000527         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1153869,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000527 DASUOM010000000
VERSION     DASUOM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153869"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="potE"
                     /locus_tag="VFY27_10965"
     CDS             complement(<1..960)
                     /gene="potE"
                     /locus_tag="VFY27_10965"
                     /inference="COORDINATES: protein motif:HMM:NF007938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HEX5766079.1"
                     /translation="MQEQKKMSLMQLTFIVAVNMMGSGIIMLPTNMAQVGAISLLSWI
                     VTAVGSMAIAYGFAQAGVFNDRSGGMAAYAEDAYGRSGYFQVFFLYFLSLAIGNVAIA
                     ISAVGYLATFFPWLSSTPISTCIGVIGLLWLTTFANFGGPSVTGRIGSITVWGVIIPV
                     GTLSLIGWFWFSTDTFAAAWNPQGLSLMEGMGSSVALTLWAFLGMESAAQNSAAVENP
                     KRNVPLACMFGTLGAAVIYVLSTTVIQGIVPNAELADSTGPFGLAFAKMFNPTVGSVI
                     MALAVVACLGSLLGWQFTIAQTGKAAAEDRMFPALFARVNRMGA"
     gene            complement(996..>2597)
                     /locus_tag="VFY27_10970"
     CDS             complement(996..>2597)
                     /locus_tag="VFY27_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEX5766080.1"
                     /translation="DRNNHKSLHQGALVKAGAIPIFLPTARNSFGMIGAVDWDAWDEQ
                     YLREQIRKHPLVEDPERHAMERPFRLACIQLATYDGTVYNVRKVLDKIGSLCDYVLWD
                     EAWIGYNAFHPLFTDHSPMRLKNLGPDMPGLFSTQSVHKQGAGFSQASQIHKRDEHIR
                     GQPRFIDHKRFNESFLMHASTSPFYPLFAALDVNAKVHDGKAGEMLWDRCIELGIETR
                     KKLREFVRYYTESGRDAQERWFFDPFVPDVVTIRGSDVTDDTTGAAWEDLPTEVIKRE
                     QQCWTFDPESAWHGYAGYVNGYAMVDPNKLMLLTPGINRQTGDYLDFGVPATVVANYL
                     REQRIVPEKCDLNSILFLMTPAEDESKLNTLIAKLVKFKELWDRDAALGEVLPTLYEQ
                     NRERYAGYSLRQTCIEMHDFYKEANVKELQKQCFRASSFPELAMSPKKAYEALVANEV
                     DYVPLDEGVRNRVAATLALIYPPGIGVLVPGERWDERARPMLDYFLTFEESFNRFPGF
                     NYEVQGVYQETVDGRIKFHTYVVRE"
BASE COUNT          520 a          789 c          783 g          505 t
ORIGIN      
        1 tgcacccatg cggttcaccc tggcaaagag cgcggggaac attctatctt ccgcggccgc
       61 cttgccagtc tgggctatgg tgaactgcca gccgagcagc gatcccaggc aggctactac
      121 ggcgagcgcc atgatcaccg aacccacggt cgggttgaac atcttcgcga atgcgagccc
      181 gaacggtccg gtcgagtcgg ccagctccgc attcggcacg ataccctgga tgaccgtggt
      241 ggaaagcacg tagatcaccg cagcgcccag ggtgccgaac atgcaggcga gcggcacgtt
      301 gcgcttcgga ttctcgacgg cagcggaatt ctgcgccgcc gattccatgc ccaggaaggc
      361 ccacaacgtg agcgcaacgc tcgaacccat gccctccatc aggctcaatc cctgcggatt
      421 ccaggccgca gcaaaagtgt cggtgctgaa ccagaaccag cctatcagcg agagcgttcc
      481 gaccgggatg atgacgcccc agacggtgat cgatccgatt cgaccagtga cgctcggtcc
      541 gccgaagttg gcaaacgtgg taagccacag aaggccgatc acgccgatac acgtcgaaat
      601 cggcgtcgat gaaagccacg ggaagaacgt tgccaggtag ccaacggccg atatcgcgat
      661 cgcaacgttg ccgatggcga gtgacaggaa gtacaggaag aagacctgga agtagccgga
      721 ccggccgtac gcgtcctcgg cgtatgccgc catccctccg ctgcggtcgt tgaaaacgcc
      781 ggcctgcgca aagccgtacg cgatggccat cgatccgacc gcggtaacga tccacgagag
      841 aagggaaatc gcgccgacct gtgccatgtt ggtgggcagc atgatgatcc cggagcccat
      901 catgttcacc gcaacgatga acgtaagctg catcaggctc atcttctttt gctcttgcat
      961 aacgtctctc ctgcaatcat ccatccgcgg tgcggctatt cccggaccac gtaggtatga
     1021 aacttgatac ggccgtcgac cgtttcctga tagacacctt gcacttcgta gttgaatccg
     1081 gggaagcggt tgaacgactc ttcgaacgtg agaaaataat ccagcatcgg ccgcgccctt
     1141 tcatcccagc gctctccggg aacgaggact ccgatccccg ggggataaat cagggcgagc
     1201 gtggcggcga cccggttacg cacaccttcg tcgagcggga catagtcgac ttcgttcgcg
     1261 accaacgctt cgtacgcttt cttcggcgac atcgcgagtt cgggaaagct gctggcgcgg
     1321 aagcactgct tctgcagttc cttgacgttg gcctccttgt agaagtcgtg catctcgata
     1381 caggtctgac gcaacgaata cccggcgtaa cgttccctgt tctgctcgta gagcgtcggc
     1441 aggacctctc cgagcgcggc gtcgcgatcc cagagttcct tgaacttgac gagcttcgcg
     1501 atcagggtat tgagcttgct ctcgtcctcg gcgggcgtca tgaggaaaag aatgctgttc
     1561 agatcgcact tttccggcac gatgcgttgc tcgcgcaggt agttggccac tacggtcgcg
     1621 ggcacgccga aatccaggta atcgcccgtc tgccgattga tccccggcgt cagcagcatg
     1681 agcttgttcg ggtccaccat cgcgtagccg ttcacatacc cggcgtaacc atgccaggcg
     1741 ctctcgggat cgaaggtcca gcattgctgc tcgcgcttga tcacctcggt cggcaggtct
     1801 tcccaggcgg cgccggtagt gtcgtccgtg acgtcggaac cacgaatggt taccacgtcg
     1861 ggtacgaatg gatcgaagaa ccatcgctcc tgggcgtcgc ggccggattc cgtgtaatag
     1921 cggacgaatt cgcgcagttt cttccgcgtt tcaatgccga gctcgataca gcgatcccaa
     1981 agcatttcgc ccgctttgcc gtcgtgaacc ttcgcattca catcgagcgc cgcgaacagc
     2041 gggtagaagg gcgaagtcga agcgtgcatc aggaacgact cgttgaagcg cttgtgatcg
     2101 atgaaacgcg gctgtccccg aatgtgctcg tctcgcttat ggatctggga agcctgcgaa
     2161 aacccggcgc cctgcttgtg tactgactgg gtggagaaca gcccgggcat gtcgggcccg
     2221 aggttcttca ggcgcatcgg actgtgatcg gtgaataggg gatgaaaggc gttgtagcct
     2281 atccatgcct cgtcccagag cacgtagtcg cagaggctgc cgatcttgtc caggaccttg
     2341 cgaacgttgt agaccgtccc gtcgtacgtg gcgagctgga tgcacgccag ccgaaacgga
     2401 cgctccatgg cgtggcgctc gggatcctcg acgagcgggt gtttgcggat ctgctcgcgc
     2461 aggtactgct cgtcccacgc gtcccagtcg accgcgccga tcatgccgaa cgaattgcgt
     2521 gcggtcggca ggaagatggg aatcgcgccc gccttgacca gagcgccctg gtgcaacgac
     2581 ttgtggttgt tgcgatc
//
LOCUS       DASUOM010000528         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_550315,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000528 DASUOM010000000
VERSION     DASUOM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550315"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFY27_10975"
     CDS             complement(<1..207)
                     /locus_tag="VFY27_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766081.1"
                     /translation="MNANVSPAHTPMMQQYVRIKAEYPDMLLFYRMGDFYELFYDDAR
                     RAARLIDITLTQRGKSAGEAIPMAG"
     gene            314..1468
                     /locus_tag="VFY27_10980"
     CDS             314..1468
                     /locus_tag="VFY27_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5766082.1"
                     /translation="MKILHIETGRHFYGGAQQVIYLLNGLRLQNVANVLACSPDTEVD
                     TRAREAGAKVYNVASAGDLDLSLAWWLMKIMRWEKPDVVHCHSRRGGDFLGGAAAALA
                     RLPAALSRRVDHPEPQWIAKLRYRPFRKVIAISENVAAVLRENGVDEERLAIIRSAVD
                     VERFREPPARKELLEEYGLDDGHFIIASVGQLIPRKGHRYLLEAMASLSRRLPRARLI
                     VFGQGDLEAELVEQASRLGLDDVVRFAGFREDLDRYMGAFDLVVHPALREGLGVSMLK
                     AAAAGVAVVAFDLAGSREAVVNGRTGILVRPKDTAGLEQAILLLAENRVLRETYGKAA
                     RARMRDEFSIATMVDKHIELYESLLSDHEHEPKGHEPAGEGQPDASQRHD"
     gene            1446..1949
                     /locus_tag="VFY27_10985"
     CDS             1446..1949
                     /locus_tag="VFY27_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX5766083.1"
                     /translation="MHRSDTIEESARALVEALLDAAKTVAAAESCTGGWVAKAITDIA
                     GSSGCFACGIVSYSDEAKEALLDVDPATLKEHGAVSEPTVREMAAGVLDLSGADLAVA
                     ISGVAGPSGGTPDKPVGTVWFSWASRSAEGCVSVDAVLHRLKGNREAVRRQSVVLALD
                     GVRERIG"
     gene            1952..2461
                     /gene="thpR"
                     /locus_tag="VFY27_10990"
     CDS             1952..2461
                     /gene="thpR"
                     /locus_tag="VFY27_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5766084.1"
                     /translation="MAERRLFFALWPSDRQRELLRDTLRPVMSSIEGDAADRRNWHVT
                     LVFIGGFPEEQVPFLQAATGEIAMDPVRLRFDRVEYWQRARVAALMPLATPASLETLV
                     QSLQAKLHAFNIEPEQRLYRPHITVARRARAFETVQLTRPVDLEWQEFELIESTSGPG
                     GVSYSPLKQ"
     gene            2591..>3235
                     /gene="recA"
                     /locus_tag="VFY27_10995"
     CDS             2591..>3235
                     /gene="recA"
                     /locus_tag="VFY27_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5766085.1"
                     /translation="MDENRKKALAAALGQIEKQFGKGSVMRLGDGGPAKDIEAVSSGS
                     IGLDVALGIGGLPRGRVIEIYGPESSGKTTLTLQVVAEAQKLGGTAAFVDAEHALDPS
                     YAEKLGVNVDELLVSQPDTGEQALEITDMLVRSGAVDIIVVDSVAALTPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGNIKRSNAMVIFINQIRMKIGVMFGSPETTTGGNAL"
BASE COUNT          628 a          962 c         1071 g          574 t
ORIGIN      
        1 gcccgccatc ggaatcgctt caccggcaga cttgccgcgc tgcgtcagcg tgatgtcgat
       61 gagcctggct gcgcgcctgg catcgtcgta gaagagctcg tagaagtcgc ccatgcggta
      121 gaagagcagc atgtccgggt actcggcctt gatgcgcacg tactgctgca tcatgggcgt
      181 gtgggcgggc gagacgttcg cgttcatgta cttgctggcg gtcgcgatcc cacttccgct
      241 tcccctgtta caggccacta caatgaaagc gcctcatgct agcctctgac tttggcggag
      301 tcgatggaca acaatgaaaa tactgcacat cgagacgggc cggcacttct acggaggcgc
      361 gcagcaggtc atctacctgc tgaacgggct ccggctgcag aacgttgcca acgtgctcgc
      421 gtgttccccc gataccgagg tggacacgag agcgcgagag gctggcgcaa aggtatacaa
      481 cgtggcttcc gccggcgacc tcgatctcag ccttgcatgg tggctcatga aaatcatgcg
      541 ctgggaaaag ccggacgtcg ttcattgcca cagccgccgt ggcggcgatt tccttggcgg
      601 tgcggctgca gcactcgcgc gcctgcccgc cgcgctgtcc cgccgtgtcg accacccgga
      661 accgcaatgg atcgcgaagc ttcgataccg gccctttcgc aaggtcatcg cgatctcgga
      721 gaacgtagcg gcggttctcc gggagaacgg cgtggacgag gaacgccttg cgatcatccg
      781 cagcgcggtg gatgtagaac gcttccggga gccgcccgcg cgaaaggagc ttttggaaga
      841 gtacgggctc gatgacgggc atttcatcat cgcttccgtc gggcagctca tcccgcggaa
      901 gggacatcga tacctgctgg aggcgatggc gagcctgtcc aggcggctgc cccgcgcgag
      961 attgatcgtc ttcggccagg gagacctgga ggctgaactc gtggagcagg catcgcggct
     1021 gggactggac gatgtcgtcc ggttcgccgg ttttcgggag gatctcgacc gctacatggg
     1081 agcattcgac ctcgtcgtgc atcccgccct gcgggaagga ctgggcgtgt cgatgctgaa
     1141 agcggccgct gccggtgtcg cagtggtagc cttcgaccta gcaggctcgc gcgaagctgt
     1201 ggtaaacggc cgtacgggga tactggtccg tccgaaggac acggctggcc tggaacaggc
     1261 tatcctcctg cttgccgaaa atcgcgtcct gcgagagaca tacgggaagg ccgcgcgcgc
     1321 acgcatgcgg gacgaatttt ccatcgcgac aatggtcgac aagcacatcg agctgtacga
     1381 gtcgctgttg agcgaccacg aacatgaacc aaagggacat gaaccggcag gtgaaggaca
     1441 accggatgca tcgcagcgac acgattgaag aatccgccag agcgctggtt gaagcactgc
     1501 tggatgcggc gaaaaccgta gcggccgcgg aatcctgcac cggcggctgg gtcgccaaag
     1561 cgattaccga tattgccggc agctcgggct gtttcgcctg cggcatcgtc agctacagcg
     1621 acgaggcgaa ggaagccttg ctggacgtcg atccagcgac cttgaaggag cacggtgctg
     1681 tcagcgagcc taccgtgcgg gaaatggccg ctggcgtgct cgacctgagc ggcgccgacc
     1741 tggcggtcgc catcagcggc gtcgcagggc ccagcggagg aacgccggac aagccggtcg
     1801 gcaccgtgtg gttctcctgg gcctcgcgct cggcggaagg ctgtgtctcc gtggacgccg
     1861 tcctgcaccg tctgaaaggg aaccgcgaag cagtgcggcg ccagagcgtg gttctggcgc
     1921 tggatggcgt gcgggagagg attgggtaag gatggctgaa cggagactgt ttttcgcatt
     1981 gtggccgtcg gaccggcaac gggagctgtt gcgcgacacg cttcgtccgg taatgagctc
     2041 gatcgaaggc gatgccgcag accggcgcaa ctggcacgtc acgctggtat tcataggtgg
     2101 tttcccggag gagcaggttc cgttcctgca ggcggcgacg ggggaaatcg caatggaccc
     2161 ggttcgcctg cgcttcgacc gggtggaata ctggcagcga gcaagggtcg ccgcactgat
     2221 gccgcttgcc acgccggcct cgctcgagac gttggtgcag tcgctgcagg cgaaactcca
     2281 cgcgttcaat atcgagcccg aacagcgtct ctacaggccg cacatcacgg tcgcgcggcg
     2341 cgcaagagcg ttcgaaaccg tgcaactgac gcgtccggtg gacctcgagt ggcaggagtt
     2401 cgaactcatc gaatccacct ccgggcccgg cggtgtcagc tatagcccgc tgaaacaatg
     2461 agtttgatcg ttctcctcgg cgattcttca cagattgccc cgattccctc tcgcaatccc
     2521 gctgccgggc gcagtcgatt tgtgaaataa tccgcatctt cgaaaacggc tcccggacgg
     2581 cattaaaacg atggatgaga atcgcaagaa agcactcgca gcagcactcg gtcagataga
     2641 aaagcagttc ggcaagggct cggtcatgcg cctgggagat ggcggtcctg ccaaggacat
     2701 cgaggctgtt tccagcggct cgatcggcct tgacgttgcg ctaggcatag gcgggctgcc
     2761 gcgaggacgg gtcatcgaaa tctacggccc cgaatcgtcc ggcaagacca ccctgacatt
     2821 gcaggtcgtg gccgaggcac agaagctcgg cggcaccgcg gcgttcgtcg atgctgaaca
     2881 cgcactcgat ccctcctatg ccgaaaagct cggcgtgaac gtggacgaac tgctggtgtc
     2941 gcagccggac accggcgagc aggcgctcga gatcaccgat atgctggtgc gttccggtgc
     3001 cgtggacatc atcgtcgtcg actcggttgc ggcgctgacg ccgaaagcgg agatcgaggg
     3061 tgaaatgggc gattcccaca tgggccttca ggcgcgactg atgtcgcagg cgctcaggaa
     3121 gctcaccggc aacatcaagc gctcgaacgc gatggtcatc ttcatcaacc agatccgcat
     3181 gaagatcggc gtgatgttcg gcagcccgga aacgacgacc ggcggcaatg cgctc
//
LOCUS       DASUOM010000529         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1396335,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000529 DASUOM010000000
VERSION     DASUOM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396335"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2861)
                     /locus_tag="VFY27_11000"
     CDS             complement(<1..2861)
                     /locus_tag="VFY27_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766086.1"
                     /translation="MLVRHKSLTAPPVTSSAVRRKHTGVQRLCLSTFLVFALALVALP
                     AWAQVEGATVRGVISSPEGPASGITVRAVNVANGQSRSTSTGNDGSYVMVNLRPGTYR
                     IQVATTGEGEEVTLRVGQSAVLNFQIGAAAAEPGPDGPRPQIAEPGMPSPAPIEEIVV
                     TGVQVETFTGGEVGTSITPEMMDRLPQVNRNFLAFADLAPGVQFVQNSDGTANIRGGA
                     QHQRAVNVFIDGVSQKDYVLKGGVTGQDTSKGNPFPQSAIAEYKVITQNYKAEYPHVG
                     STAITAVTASGTNEFHGSVFGDYTNEDLRAAVPLEEQSGEKVASSQSQYGFNVSGPIV
                     RDRLHFLFAYEAKDNEDPVDIVPGGGFTAETLPPEYAALVGREVAEFKEDLYFAKLDW
                     FINDSHDISASVKYRDEISEQGFQNVDTKSFGGSIDQEDTRVQVKHTWNADAWQNEFR
                     ITYEDSLWSPNPLTNGNAERLVNASDEAILNVGGGQNLQRKGQDGWGIQNDFTVLDLE
                     WFGGHTIKTGISYKKITLNTLQQLPANPQYFYNVEFNGPGTFELVQPHRVEFGMPLTA
                     ESGAVETDNTQWGLYVQDDWYVTERLTLNIGIRWDYEETPIYVDRVTPPAVAAALQSW
                     PNIANANYDISNWISTGGNRDYDGDNIAPRLGFSYDIGEDAMHTIYGGYGRSYDRNQF
                     DFVQLEVTNGTFGRVSFFFEGDPANPCEGTNCVPWDPAYLTPEGLDALVASTGLANPP
                     TEIFLLNNELKTPYSDQFSIGLRSTWGAWDTDLTFSHVESKNGFNWLLGNRRENGEFF
                     APGAIWGAPFGFTPPGFSNVLLSSNDLETEADSIFLKLDRPHQETWGISFAYTFTDAE
                     ENRNFDGTFTLDYPTIEGYGRRDAVGIADHRLVASGTYDLPWEVLLSGKLTLDSGVAF
                     QYTDCLAGPDQCVLRRIEPDDAEMRQLDLAVSKTFNFGFL"
     gene            3417..>5391
                     /locus_tag="VFY27_11005"
     CDS             3417..>5391
                     /locus_tag="VFY27_11005"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5766087.1"
                     /translation="MGTHPRGAIGSPSASSPNLREADTELDPAVLLAALQSMRNGDFS
                     VRLPGDWTGLAGKIADTFNDVVTANDHMASELQRVSEAVGRRGRTRERVRCERRVGSW
                     SAMESSVNGMVDDLLWPTTAVTRSIAAVAQGDLSETVPLEVDGHRIEGEFLRSAQIVN
                     RMIEQMRVFTFEVTRVAREVGAEGTLGGQAEVRDVSGVWKDLTDNVNSMANHLTAQVR
                     NISEVTIAVANGDLSRKITVDVRGEMLQLKDAINTMVDQLRSFASEVTRVAREVGTEG
                     KLGGQATVPGVAGTWKDLTDNVNVMATNLTDQVRGIAEVATAIARGDLSRKITVDVKG
                     ELLELKDTINTLVDQLNGFAAEVTRVAREVGTEGKLGGQAKVSGVAGTWKDLTDNVNS
                     MAGNLTAQVRNIAEVTTAVARGDLSRKITVNVQGEILELKNTINTMVDQLNSFASEVT
                     RVAREVGTEGELGGQAVVPGVAGTWKGLTDNVNVMAANLTNQVREIAEVASAIARGDL
                     SRKITVDVKGEILELKNTINTLVDQLNGFAAEVTRVARDVGTEGKLGGQATVPGVAGT
                     WKDLTDNVNVMAENLTNQVRGIVKVVTAVAHGDLTQKLPVESKGEVAALAKTINSRTD
                     TLAIFADQVTTVAHEVGVEGRLGGQANVPGAAGT"
BASE COUNT         1015 a         1648 c         1707 g         1021 t
ORIGIN      
        1 aggaagccga agttgaaggt cttggaaacg gcgaggtcaa gttgtcgcat ctccgcatcg
       61 tccggctcga tgcggcgcag gacgcactgg tccggacccg ccaggcagtc cgtatattgg
      121 aaggcaacgc ccgaatcgag agtgagcttg ccggacagca gaacctccca gggcaggtcg
      181 tacgttccgc tcgcgacgag gcggtgatcg gcgatgccga ctgcgtcgcg cctgccgtac
      241 ccttcgatcg tcgggtagtc cagggtgaag gtcccgtcga aattacggtt ctcctcggca
      301 tccgtgaagg tgtaggcaaa cgaaatgccc caggtctcct gatgtggccg gtccagtttg
      361 aggaagatcg agtcagcctc tgtctccagg tcattactgg aaaggagcac gttggaaaac
      421 ccgggcggcg tgaagccgaa cggcgctccc cagatcgccc cgggcgcgaa aaactccccg
      481 ttctcgcggc ggttgcccag cagccagttg aagccgttct tgctttcgac atgtgaaaag
      541 gtgaggtcgg tatcccacgc gccccaggta ctccgcaggc cgatgctgaa ctggtcggag
      601 taaggggttt tcagttcgtt gttcagcagg aatatctctg tcggtggatt cgcgagtccc
      661 gtgctcgcca ccagcgcgtc caaaccctcc ggcgtcagat aggcggggtc ccacggaacg
      721 cagttcgttc cctcgcaggg attggcagga tcgccttcga agaagaacga aacccggccg
      781 aacgtaccat tcgtaacctc gagctgcacg aagtcgaact ggttgcggtc gtaagaccgt
      841 ccgtagccgc cgtagatggt gtgcatggcg tcttcgccga tgtcgtagga aaaccccagt
      901 cgcggcgcga tattgtcacc gtcgtagtcc cggttgcccc cggtgctgat ccagttgctt
      961 atgtcatagt tggcgttcgc gatgttcggc cagctctgca gcgccgccgc caccgccggc
     1021 ggcgttaccc tgtcgacgta gatgggcgtc tcctcgtagt cccagcgtat gccgatgttg
     1081 agtgtcagtc gttccgtcac ataccagtcg tcctggacgt acaggcccca ttgcgtgttg
     1141 tcggtttcga cggcgccgct ctcggcggtc agcggcatgc cgaactccac gcggtgtggc
     1201 tgcacaagtt cgaacgtgcc gggtccattg aattcgacgt tgtagaagta ctgcgggttt
     1261 gccggcaact gctgcaaggt gttcaacgtg attttcttgt aactgatacc cgtctttatc
     1321 gtgtgcccgc cgaaccattc caggtccagc acggtgaagt cgttctggat tccccagccg
     1381 tcctgccctt ttctctgcag gttctggccc ccacccacat tcaggatggc ttcatcggag
     1441 gcgtttacca gtcgctcggc gttgccgttc gtgagcgggt tgggactcca cagggaatcc
     1501 tcgtaagtaa tacggaactc gttctgccac gcgtccgcgt tccaggtgtg ctttacctgt
     1561 acccgtgtgt cctcctggtc gatgctgccg ccgaaagact tcgtgtcgac attctggaag
     1621 ccctgctccg aaatctcatc gcgatatttg accgacgcgg aaatatcatg cgagtcgttg
     1681 atgaaccagt cgagcttggc gaaataaaga tcttccttga actcggcgac ctccctgccg
     1741 accagcgcag cgtactccgg cggcagcgtt tctgcggtga agcctccgcc gggaacgata
     1801 tcgaccgggt cctcgttatc ctttgcctcg taagcgaaca ggaagtgcag cctgtcccgg
     1861 acgatcgggc ctgatacgtt gaagccgtac tgcgactgtg aagacgcgac cttctctcca
     1921 ctctgctcct ccagcggcac cgccgcccga aggtcttcgt tcgtatagtc cccgaacacg
     1981 ctgccgtgaa attcgttcgt gccggatgcc gtcacagccg tgatagcggt actgcccacg
     2041 tgcgggtatt cagccttgta gttctgcgtt atcaccttgt actcggcgat cgcggattgc
     2101 gggaaggggt ttcccttgct cgtgtcctgc ccggtcacgc cgcctttcag cacgtagtcc
     2161 ttctggctga cgccgtcgat gaagacgttg acggcgcgct ggtgctgtgc gccgccgcgg
     2221 atgttcgccg taccgtcgga gttctgcacg aactgcaccc cgggagcgag atcggcgaac
     2281 gcgagaaagt tgcggttgac ctgcggcagc cggtccatca tctccggcgt gatcgacgtg
     2341 cctacttcgc cgccggtgaa ggtctcgacc tgcaccccgg tcaccacgat ctcctctatc
     2401 ggcgccgggc tgggcattcc gggctcggcg atctgcggac gcggcccgtc cggcccgggc
     2461 tcggcggcgg ccgcaccgat ctggaagttg agcacggcgc tctggccgac tcgcagggtg
     2521 acctcttcgc cctcgccggt cgtcgccacc tggatgcgat aggttccggg gcggaggttc
     2581 accatcacat aggaaccgtc gttgcccgtg gacgtgctcc tggactggcc attggcaacg
     2641 ttgactgcac gaacggtgat accgctcgcc gggccttccg ggctcgagat gacgccgcga
     2701 acagtggcgc cctcgacctg agcccacgcc ggcagcgcca ccagtgccag ggcaaacacg
     2761 agaaacgtgg aaaggcatag ccgctgcacg cccgtatgtt tgcggcgtac tgcactcgat
     2821 gttacgggcg gcgctgtaag ggatttgtgc cggaccagca tgacaacgct cctgctattg
     2881 gcgtgttcat gctccccccg ggacgccgct cgtgaaacgg cgtttgcaca tggttatggt
     2941 aaaccagcgg tttgcggagg tgcaacagcg gagccttgcg gcagcgggct gtgcaaggtt
     3001 tcgttgcact aacaagacag cgggtcggca ggagcccacg tcgaaaggaa gcggcacgct
     3061 gccggatccc tcgccgtgtg ggcggcgaat gccgggcgag tgagatacac accagcgaac
     3121 tcgtccgcgt tgcgtgctcc tccagcgact ctttcgggcg ctggcgaagt gcgcactggt
     3181 ctctgcgatt ggtatttacg gcgcatttat taccgctacc ataaaaatcg tgttgcgcct
     3241 tagttttgcc ggttgtgatg acacctcgct ccggtgtacc ttctccaagc gcgaattcgc
     3301 ggagcccgct cgtctcttta tgttaaagag gcgagcgccc tcgccagcag tgtcacaagc
     3361 gctccggtct ttaagccccg tctgggccct cgcaattaca attaacggat aacgctatgg
     3421 gaacacaccc aaggggtgct attggctcac cgtccgcttc atcgccgaat ttgcgggagg
     3481 ccgacacgga acttgacccg gccgtactgc tcgctgctct gcaatcaatg cggaatggcg
     3541 atttttcggt gcgcctgcca ggagactgga caggcctcgc gggcaagatc gcggacacgt
     3601 tcaatgatgt cgtaaccgcg aatgaccaca tggcctcgga actgcagcgc gtcagtgaag
     3661 ccgttggccg tcgcggcagg acgcgtgaac gggtgcgctg cgaacgccgc gtaggttcgt
     3721 ggagcgcgat ggaatcctcc gtgaacggca tggtggacga tctcctctgg ccgaccactg
     3781 ccgtaacgcg cagcatcgcc gccgtagccc agggcgatct ctctgaaacc gttcccctcg
     3841 aggtcgacgg acatcggatc gagggcgagt tccttcgttc cgcgcaaatc gtcaaccgca
     3901 tgatcgagca gatgcgggtg tttacatttg aagtaacgag agtggcgcgc gaagtcggcg
     3961 ccgaaggaac gttgggcggc caggccgagg tccgggacgt gtcgggcgtc tggaaagatc
     4021 tcacggataa cgtcaattcg atggcaaatc acctcaccgc ccaggtgcgc aacatctccg
     4081 aggtgactat cgcggtcgca aacggcgacc tgtcgagaaa gattaccgtg gacgtccgcg
     4141 gcgagatgct ccagctgaag gacgccatca acacgatggt cgaccagctg cgttccttcg
     4201 cctcggaggt gacgcgcgtc gcgcgcgagg ttggcaccga aggcaagctc gggggccagg
     4261 ccaccgtgcc tggcgtcgcc ggaacctgga aggacctgac agacaacgtg aacgtgatgg
     4321 cgaccaacct caccgaccag gtgcgtggca tcgcggaggt cgcgaccgcc atcgcccgcg
     4381 gcgacctgtc gcgcaagatc acggtcgacg tcaagggcga actgctggag ctcaaggaca
     4441 ccatcaacac gctcgtggac cagctgaacg gcttcgccgc ggaagtgacg cgcgttgcgc
     4501 gtgaggtcgg tacggaaggc aagctcggcg gacaggccaa ggtttccggt gttgccggca
     4561 cctggaaaga cctgaccgac aacgtgaact cgatggcagg caacctgaca gcccaggtac
     4621 ggaatatcgc cgaggtcacg accgccgtcg cacgcggcga cctgtcgcgc aagatcacgg
     4681 tgaacgtgca gggcgagata cttgaactga agaacacgat caacacgatg gtcgaccagc
     4741 tcaacagctt cgcctcggaa gtgacgcgcg tcgcgcgcga ggtgggcacc gagggcgaac
     4801 tcggcggaca ggccgtcgtg ccaggcgtcg caggcacctg gaagggactc accgacaacg
     4861 tgaacgtcat ggccgcaaac ctgacgaacc aggttcgcga gatcgccgag gtggcgtcgg
     4921 ccatcgcacg cggcgacctg tcgcggaaga tcaccgtcga cgtcaagggc gagattctcg
     4981 agctcaagaa caccatcaac acgctggtgg accagctgaa cggctttgca gccgaggtga
     5041 cgcgcgttgc gcgcgatgtt ggtacggaag gaaagctcgg cggccaggcg acggtgccgg
     5101 gcgtcgccgg cacgtggaag gacctgacgg acaacgtgaa cgtaatggcc gagaacctga
     5161 cgaaccaggt gcgcggcatc gtcaaggtgg ttaccgcggt cgcccatggc gacctgacgc
     5221 agaaactccc cgtcgagtcc aagggcgaag tagcggcact tgccaagacc atcaacagca
     5281 ggaccgacac gcttgcgatc ttcgccgacc aggtcacgac ggtggcgcac gaagtgggag
     5341 tggaggggcg cctcggcggc caggcgaacg tgccgggagc cgcgggcacc t
//
LOCUS       DASUOM010000530         6271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_671407,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000530 DASUOM010000000
VERSION     DASUOM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671407"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFY27_11010"
     CDS             <1..386
                     /locus_tag="VFY27_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5766088.1"
                     /translation="PRELVARIQSLLRRVGPGETHPQVMRFAGIEVDTAARTVVVDSQ
                     PVELTSMEFELLAILARRAGKKVTRDEILSALRGIDAAILTRSVDIMVSRLRQKLGDT
                     IKPPRFIQTIWGTGYSFIGRRVLDV"
     gene            379..1743
                     /locus_tag="VFY27_11015"
     CDS             379..1743
                     /locus_tag="VFY27_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766089.1"
                     /translation="MFDRLRRSLSFRLLAIFLTLAVLFVYGSIEAVRWIYGNDDLREL
                     ISGHLALHVKYVRDDIGDPPRIDRAIAITKTVPVDIRIAGPGINWASDPDFPELEELD
                     FGASAIFSEDPGAWLNEIEDVEFAKLGKHRFLKIDQGTFATIVSTPRIAETPSGPSLL
                     AIIVGSGLLWLFIAYLCVRWLFRPIDSIREGAARIGQGDLSYRIEGYRQDQLGELAED
                     VNKLASDVRNMLDAKRQLLLGISHELRSPLSRLRLSLEFLADGAQKESLRSEIAEMEQ
                     IVATLLEAERLNTRHAALNRTDVQVRDLVEQLIEDYFVRDKHRIELAFEDDALVANVD
                     DARLILLVKNLLNNALRYSDPDKGPVRLAVSMSGGELIIEVQDHGPGFSDEQAARIGE
                     PFFRGDPSRTRRTGGTGLGLYLTRLVAEAHGGTLSLRRDYRGGACLAVRLPVAELQRG
                     AQEA"
     gene            complement(1777..1992)
                     /locus_tag="VFY27_11020"
     CDS             complement(1777..1992)
                     /locus_tag="VFY27_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766090.1"
                     /translation="MSGQSEITARSIRFGTCVSLGLFLSHLQGVSGLDITYTALLAST
                     LFLAFLLGEAGLAEYEKLKRKRRRKKL"
     gene            2397..3419
                     /gene="hflK"
                     /locus_tag="VFY27_11025"
     CDS             2397..3419
                     /gene="hflK"
                     /locus_tag="VFY27_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5766091.1"
                     /translation="MRQDKNVLHEILNMQRRPPQGPSGRQIALGLLVLLVVVAGFTSF
                     YTVQPEERAVIKRFGAVHGITDPGLHFKLPFGIDSVQKVATERVLKQEFGFRTDEVSG
                     SDRTRFSEQEFPDESLMLTGDLNIIEVEWVVQYRIADPIKYLYGMREPVRTLRDLTES
                     VMRRVVGNRIGSEVLTIGRVEIANDAREEIQAAMQRYDNGLHIITVELQDVVPPARVQ
                     PAFNEVNEARQELERMINEANRELNLAIPRAEGTANRVIAEAEGYATERVNRALGETA
                     RFSAVLDEYRDAPEVTRTRLYLEALHETLPRIGSVFVMEEGQVLPLLNMRGGDLQRIV
                     EPEEAR"
     gene            3416..4360
                     /gene="hflC"
                     /locus_tag="VFY27_11030"
     CDS             3416..4360
                     /gene="hflC"
                     /locus_tag="VFY27_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5766092.1"
                     /translation="MSRNLTLASVALAALFFVAINSAFVIDQAEQGIIVQFGEPIGDV
                     IAEPGLHWRVPFIQEVRRFDKRLLAWDGDVSQIPTLGREFIVVDTTARWRIADPLQFL
                     RSVRDESGARARLDDILDSVTRDIVSGTELEEIVRSADWEVDMTGLAEEEVNALAETD
                     LERPKKGRAMLERDMFAGAATQMPELGIELIDVRIKRINYIESVRAQVETRMISERQS
                     IAARFRSEGQGRSQEILGGMERDLRRIRSEAERAAQEIRGEADAEATRIYGLAFSEDP
                     EFYAFFRTLESYDALGENMTLMIDRDSDFFRYLRSTQR"
     gene            complement(4404..4604)
                     /locus_tag="VFY27_11035"
     CDS             complement(4404..4604)
                     /locus_tag="VFY27_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766093.1"
                     /translation="MTPFNIVLVVVGVGLLLLGAVSGYALFARALKISDRFGDETNVG
                     TLWGLFLLGLSAGLALLWWALP"
     gene            complement(4621..5046)
                     /locus_tag="VFY27_11040"
     CDS             complement(4621..5046)
                     /locus_tag="VFY27_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5766094.1"
                     /translation="MATEERTVWLGTPSQVINLGSFILLGLFFWLVIPLFVILWKWLV
                     VKNMKYELTTERLRLRHGVFNKKLDELELYRVRDYKLDQPFFLRIFSLANIILQTSDR
                     SHPTVIIQAIPDAENLREQLRTYVEACRTRKGVRELDVE"
     gene            5180..6262
                     /locus_tag="VFY27_11045"
     CDS             5180..6262
                     /locus_tag="VFY27_11045"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5766095.1"
                     /translation="MKSRTTRRILIIAAVIVAVGVLVRAAMPWAAARYVNNQLADMGD
                     YRGHVSDVDIALIRGAYTLRDATVTKREAQTDQPFLVLPRLDISVEWSALFDGELVGE
                     IQMQKPVANLIQGESNADTQTGAGVNWPEQVRKLFPFRFNRIEIADGTATFRAPGIEA
                     EESLNLHNLNVVLNNLTNVEQRDAPIFADFDVNGRLMENTPLAITGHINPNAEQPTFD
                     VNMSLEGAPLVGVNPWLEEFLNVDAEKGTFSMYAELAAADGRFEGYIKPLLEDAEIFR
                     LDEPSSGPLQKAWEALVGLVSGILTNPGEDQVATQIPFEGDIENPDAGILSAAINLLR
                     NAFVAAFTHSLEGTVSLEDVETGDGE"
BASE COUNT         1275 a         1903 c         1965 g         1128 t
ORIGIN      
        1 agccgcgtga acttgtggcc cgcatccaga gcttgctgcg gcgtgtcgga cccggagaaa
       61 cgcacccgca ggtgatgcga ttcgcaggta ttgaagtaga cacggcagca cgaactgtgg
      121 tcgttgacag ccagccggtg gagttgactt cgatggaatt cgagttgctc gcgattctcg
      181 cacggcgcgc cggcaagaag gtcacgcgtg acgaaatcct gagcgctctg cgcggcatcg
      241 acgcggcaat actgacgcgc tcggtcgaca tcatggtcag ccggctacgc cagaagcttg
      301 gtgacacgat caagccaccg aggttcatcc agacgatctg gggcaccggc tattccttta
      361 tcggccggcg cgtgctggat gtttgaccgt ctcaggcgat cgctatcctt tcgcctgctc
      421 gcgattttcc tgacactggc ggtactgttc gtctacggct ccatcgaggc ggtacgctgg
      481 atctacggca acgacgatct tcgggaattg atcagcggcc acctcgcgct gcacgtaaag
      541 tacgtgcgcg atgacatcgg cgatccgccg cggatcgatc gcgcgatcgc gattaccaag
      601 acggtgccgg tagatatccg catcgcaggt cccggcatca actgggcctc cgaccccgac
      661 ttccccgaac tcgaggagct ggatttcggc gcaagcgcaa ttttcagcga ggacccagga
      721 gcctggttga acgagattga agatgtggag tttgcgaagc tgggcaagca tcgcttcctc
      781 aaaatcgacc agggtacgtt tgcgaccatt gtctccacac cgcgcatcgc agagaccccc
      841 tccggccctt cacttctggc catcatcgtc ggcagcggcc tgctgtggct gttcatcgcc
      901 tatctgtgtg tgaggtggct gtttcgcccg atcgactcga ttcgcgaagg cgctgcgcgg
      961 atcggccagg gggatctctc ctacaggatc gaaggctacc ggcaggacca gctgggcgaa
     1021 cttgccgagg atgtcaacaa gctggcctcg gacgtgcgca acatgctgga tgcgaagagg
     1081 cagcttcttc tcggtatcag ccatgaactc cggtcgccgc tctcgcggct gcgtctctcg
     1141 ctggagttcc ttgcagacgg cgcacagaag gagagcttgc ggtcagagat cgcggagatg
     1201 gagcagatcg tggcgacact gctcgaggcc gaaaggctca acacacggca tgccgcgctc
     1261 aaccgcaccg acgtgcaggt ccgggatctg gtagagcagt tgatcgaaga ttatttcgtc
     1321 cgcgacaagc accgcatcga actcgccttc gaggacgatg ccctggtagc gaacgtggac
     1381 gacgcccgcc tgatcctgct ggtcaagaac ctgctcaaca atgcgctacg gtactccgac
     1441 ccggacaagg ggccggtgcg gctggcggta tcaatgtccg gaggtgaact gatcatcgaa
     1501 gttcaggatc acggtcccgg cttttcggac gagcaggccg caagaatcgg cgaacccttc
     1561 ttccgcggtg acccttcccg aacccggagg accggcggga cgggactcgg gttgtacctg
     1621 acacgactcg tcgccgaagc acacggcggg accctgagtc tgaggcggga ttatcgtggc
     1681 ggagcgtgcc tggcagtcag gttaccggtc gcagagctgc agcgcggtgc gcaggaggcc
     1741 tgacccggac tgcttgcgaa cgcgctcaac cgcgtgctaa agctttttcc tgcgcctttt
     1801 ccgcttcagt ttttcgtatt cggccaggcc cgcctcgccc agcaggaacg ccaggaaaag
     1861 cgtgctggcg agcaaagctg tataggtgat gtcgaggccg gagacgcctt gcaggtgcga
     1921 caggaagagg ccaagtgaaa cgcaggtgcc aaagcggatg gatcgggcgg taatttcaga
     1981 ctgtccagac attgcggtgc ggtttcccca ttcgttgtaa tagtcggtgg cgcaagcaga
     2041 atcgtcccac cgacacgcga atcccgcgcg aacagacttc acaactctgc gacttgcggc
     2101 aggccggcat tttccggccg gcggcgttca gtcggagtcg tcaatcggcg actgtaaatg
     2161 acctggcctg gcacaccgtg gcacgtgact tatacagtgc ctgccgaagc tgtcaaggca
     2221 aaaaccccct ccggtgacgg ccggtcccag ggtgtgaaag cggaaatccg ggcgcccgct
     2281 gggttacagt ggcgaactcg acctgattcg cgcaccggcc cggcaatccg gacgtcacgg
     2341 tttgcgccag aacaactcca aagtcatcaa caaagatcag caacaaggat cgcataatga
     2401 gacaggacaa gaacgtgctt catgagattc tcaacatgca gcgccgtcct ccacaagggc
     2461 catccggacg gcagatcgcc ctcggcctgt tggtcctcct ggtcgtcgtt gcgggcttca
     2521 cgtcgttcta taccgtgcag cccgaggagc gcgccgtgat caagcgcttc ggcgcggttc
     2581 acgggatcac ggaccctggt ctgcatttca agctgccttt cggtatcgat tcggtgcaga
     2641 aggtggcgac cgagcgtgtc ctgaagcagg agttcggctt ccgcaccgac gaggtgtcgg
     2701 gttccgaccg cacgcgtttc agcgaacagg agttccccga cgaatcgctg atgcttaccg
     2761 gcgacctgaa catcatcgag gtcgagtggg tggtgcagta ccgcatcgct gatccgatca
     2821 agtacctgta cggcatgcgc gagccggtcc ggaccctgcg ggacctgacc gagtcggtca
     2881 tgcggcgcgt cgtcggcaat cgcatcggca gtgaagtgct gacaatcggg cgcgtggaga
     2941 tagcgaacga cgcccgcgag gagatccagg ctgccatgca gcgttacgac aacggcctgc
     3001 acatcatcac ggtcgaactg caggacgtcg tgccgccggc gcgcgtccag ccggcgttca
     3061 acgaagtcaa cgaagcccgg caggaactgg aacgcatgat caacgaggcc aatcgcgaac
     3121 tgaacctcgc gatcccgcgc gccgagggca cagcgaaccg ggtcattgca gaagcggaag
     3181 ggtatgcgac tgagcgcgtc aaccgcgcat tgggcgaaac agcgcgattc agcgccgttc
     3241 tcgacgaata tcgggacgcg cccgaggtga cacgcacgcg actttacctg gaggcgttgc
     3301 atgagacctt gccgcgcatc ggttcggtct tcgtcatgga ggaagggcag gtgcttccgc
     3361 tcctgaacat gcgcggcggc gatctgcaac ggatagtaga accggaggaa gcacgatgag
     3421 caggaatctc actctcgcct cggtggcgct cgcggccctg ttctttgtgg ccatcaactc
     3481 agcgttcgtc atcgaccagg cggagcaagg aattatcgtg cagttcggcg aaccgatcgg
     3541 cgacgtgatc gcggagccgg gcctgcactg gcgcgtgccg ttcatccagg aagtacggcg
     3601 cttcgacaag cgcctccttg cgtgggacgg cgatgtcagc cagatcccga ccctcggccg
     3661 cgagttcatc gtcgtcgaca ccaccgcgcg ctggcgcatc gccgacccgc tgcagttcct
     3721 gcggtcagtg cgcgatgaat ccggcgcgcg agcgcgcctg gacgacatcc tcgattctgt
     3781 cacccgcgac atcgtctccg gcaccgagct cgaagagatc gtacgctcgg ctgactggga
     3841 agtggacatg acgggtctgg cggaggagga agtgaacgcc ctcgccgaga cggacctcga
     3901 gcgcccgaag aaaggcaggg cgatgctcga gcgcgacatg ttcgcgggcg cggcgacgca
     3961 gatgccggaa ttgggaatcg aactcatcga tgtgcgcatc aagcgcatca actatatcga
     4021 gtcggttcgc gcgcaggtcg agacacggat gatttcggag cgacaaagca tcgccgcccg
     4081 tttccgctcc gaaggccagg ggcgcagcca ggagatcctg ggcggaatgg agcgggactt
     4141 gcgcaggatt cgctcggagg ccgagcgcgc ggcccaggag atccgcggcg aggcggacgc
     4201 agaagcaacg cgcatctatg gcctcgcgtt cagcgaggat ccggagttct acgcgttctt
     4261 caggacgctg gagagctacg acgcgctggg cgagaacatg accctgatga tcgaccgcga
     4321 ttccgacttc ttccgctacc tgcgatcgac gcagcgctga tcgcggaaag tggttgcttg
     4381 aacgcgtacg ccggcgggcg gcgtcagggc aacgcccacc acagcagcgc caggccggcg
     4441 gaaaggccca gcaggaacag cccccacaaa gttcccacgt tggtctcgtc gccgaacctg
     4501 tcggagatct tcagcgcgcg tgcgaacagg gcatacccgg atactgcgcc caacagcagc
     4561 aggccgaccc cgaccaccac caggacgata ttgaaaggcg tcattcgttg tccccttcgg
     4621 ctactcgacg tccagctcgc ggacaccctt cctggtcctg cacgcctcga cgtaggtacg
     4681 cagctgctcc ctcaggtttt ccgcgtccgg aatagcctgg atgatcacgg tcggatggct
     4741 cctgtccgag gtctgcagga tgatgtttgc cagcgagaag atgcgcagga aaaatggctg
     4801 gtcgagcttg taatcgcgta cccggtacaa ctcgagctca tccagcttct tgttgaacac
     4861 gccgtgacgc aggcgcagcc gctctgtcgt cagttcatac ttcatgttct tgacgaccag
     4921 ccatttccac agaatcacga aaaggggaat gaccagccag aagaacaagc ccaacaatat
     4981 gaaagagccc aggttgatga cctgtgatgg cgtgcccagc cagactgtcc tttcttctgt
     5041 tgccatagcg ctccctccgc cgccggttcg cgggttccgc ggcttctcaa gcgcggtatt
     5101 ctaaccctat ctggcataca tcttgctcgg tcttccctgc ggccgctcac atctcgcggc
     5161 cctggcgaag ggcaggccca tgaagtcgag gacaacgcgt cgaatcctga tcatcgcagc
     5221 cgtgatcgtg gcggtggggg tgctggtacg cgcggctatg ccgtgggcgg cagcgcgcta
     5281 tgtgaataac cagctcgccg acatgggaga ctaccggggg cacgtctctg atgtagacat
     5341 cgccctcatc cgtggggcgt acacgctgcg cgacgcgaca gtgacgaaac gggaagcaca
     5401 gacggaccag ccgttcctag tgctgccgcg gctcgacatt tcggtggaat ggtcggcact
     5461 gttcgacggc gagctggtcg gcgagatcca gatgcagaaa cccgtcgcca acctgatcca
     5521 gggtgagagc aatgcggaca cgcagaccgg tgcgggagtg aactggcccg agcaggtgcg
     5581 aaaactcttt ccattccgtt tcaatcgaat cgagatagcc gacggtacgg caacgtttcg
     5641 cgcacccggg atcgaagcgg aggaatcgct gaacctgcac aacctgaacg tcgtgctcaa
     5701 taatctcacg aacgtggagc aacgcgacgc acccatattt gcggatttcg acgtcaacgg
     5761 caggctcatg gagaacactc cgctggcgat cacgggacac atcaatccca atgcagagca
     5821 acccaccttc gacgtcaaca tgtcgcttga aggcgcgccg ctggtcggag tgaacccgtg
     5881 gctggaggaa ttcctcaacg tcgacgcaga gaaggggacc ttctcgatgt atgccgaact
     5941 cgccgccgcc gatggccgct tcgaaggcta tataaagccg cttctcgagg atgccgagat
     6001 ctttcgcctg gatgagccgt cgagcggccc gctgcagaaa gcatgggagg cgctggtcgg
     6061 tctggtcagc gggatactca cgaaccccgg cgaagaccag gtggcgacgc agattccctt
     6121 cgaaggcgat atcgagaacc ccgatgccgg catactctcg gccgcgatca acctgctgcg
     6181 gaacgcgttc gtcgccgcgt tcacgcactc gctggaaggt accgtgagcc tcgaggacgt
     6241 agaaacgggc gatggcgaat agattcctca a
//
LOCUS       DASUOM010000531         4620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1269991,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000531 DASUOM010000000
VERSION     DASUOM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269991"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFY27_11050"
     CDS             complement(<1..354)
                     /locus_tag="VFY27_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766096.1"
                     /translation="MGALSLLPATVAIVLAFVTRNTVFSLAVACFIGVLVAGQGLMGF
                     PNLLKDALGTPAFSWIFLLELFIGMLIAFFQRTGAIRNFSAWVASLNMTRVRVQLVAW
                     IMGMFVFFSDYFSPLF"
     gene            complement(452..1756)
                     /locus_tag="VFY27_11055"
     CDS             complement(452..1756)
                     /locus_tag="VFY27_11055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5766097.1"
                     /translation="MSNRTEAASFPRYRQRCGWNALLPPRRVNAELEEDLRCDVVIVG
                     AGYTGLAAARRFQELEPEARIVVLDASEVGEGSPGRNSGFMLEIALADDADPRQLERM
                     TACNRLIADAMTGLKRLVDTNEIACDLSRTGTYRAAAGAAGIAALRNYRAFLDAACLP
                     YEPLDRQQLHDRLGTRFYKEGLYSPHCYLVQPAALIRGLADSLPPRVVLHENSPVVAI
                     ERKGGTWTARTPKASVHAPLLILANNAFCKNLGIAGSRIVAMYTYAAMTKPLSPGSLD
                     SLGSDLAWGLLPAHRLGSTLRRTGDGRLLIRSLYGYEAEADSGTVSVMLKSALERRYP
                     QLRELDFSSVWSGATGFTLNGAPVWGEYAPGLFVAAGCNGGGVVKGTLFGRLLAEVAL
                     GFGAPDIAALFGRAAWMPPEPLRRLGFNVVSRVERYKGRAEA"
     gene            1831..2871
                     /locus_tag="VFY27_11060"
     CDS             1831..2871
                     /locus_tag="VFY27_11060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5766098.1"
                     /translation="MINTDVVIVGAGPCGLFQVFELGLLGLKAEVVDSISKPGGQCAE
                     LYPDKPIYDIPAIPVCTGEELTNSLLKQIEPFNCGMHLGEEVIIVRRQEDDTFFVETD
                     ARKRFTARAVVIAGGVGSFQPRPLRVPDAELYEDRSLHYRVRDASQFHDRRIVILGGG
                     DSALDWVLALVGKASHITLVHRRDEYRAAPASVGKMRSLVADRRMDLIEHGKAVAITG
                     AEGRMSAITVNVRAGDSAEERSLVVPAEHALVFFGLSPKLGPIADWGLDINRKTINVD
                     TERFETSEPGIYAIGDINQYPGKKKLILCGFHEAALAAFAIKQRLEPDKKVHVQYTTT
                     SPLMHKRLGLPD"
     gene            complement(2956..3924)
                     /locus_tag="VFY27_11065"
     CDS             complement(2956..3924)
                     /locus_tag="VFY27_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5766099.1"
                     /translation="MRFILTAISAVMLSVLPALAISAPAVQVDVIKQPYGGARNVAEI
                     SMNPDYIHASGLERLIESWGGQLIRPVQDVRLDAAQERQYGEWNRMALANSNMAKLVR
                     EGRSDDVLTIGLEANCNVLLGMLAGLKYDEEGHTRRVGLVFIDAHGDFNVPETTLSGM
                     LGGMPVAVAAGHALHNIRKTSGLTEPLPMEHIVWGGVRDLDPLEAERFREYEVQQFSV
                     EDVRSLSGALRRQMDDLASRVDAIYVHIDMDVLDPEEVPGHDLAVTDGPSSRDLAAAM
                     TLMFRNPKTVALGIASTPAGPRDPAGVSRQAALNLIEGAIKGVQSR"
     gene            complement(4048..>4620)
                     /locus_tag="VFY27_11070"
     CDS             complement(4048..>4620)
                     /locus_tag="VFY27_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766100.1"
                     /translation="NIRAIGLPEWSEDDQQFAKALQELMGADEITGLAAELDGIEEPR
                     EEPVSGGSDDIGDVSWNVPTVTLRFPSNVEGMTFHHWSSAMAMATPIAHKGATAGAKV
                     IAATMLDLLQDDELRREAWRYFREEQLAGDEYVPFISRDDPPAIEKNEETMNSYRNRM
                     REFYYDPSRYDTYLEQLGIDYPQLERPDGD"
BASE COUNT          876 a         1428 c         1407 g          909 t
ORIGIN      
        1 gaagagcggg ctgaaatagt cactgaagaa cacgaacatg cccatgatcc aggccaccag
       61 ctgcactcgc acccttgtca tattcaggct ggcgacccac gcgctgaagt tgcggatcgc
      121 cccggtgcgc tgaaagaacg cgatcagcat cccgatgaat agttcgagca ggaaaatcca
      181 tgagaacgcg ggggtgccaa gggcgtcctt gagcaggttc gggaatccca tcaatccctg
      241 gccggctacc agcacgccga tgaagcaggc cacggccagc gagaacaccg tgttgcgggt
      301 gacaaacgca agcacgatag ccacggtcgc aggcagcagc gaaagcgcgc ccaaagcctc
      361 aacttggtcc acgggatccc ctgatgtttt ctaatcgttg ttcagtgacg ttactggaag
      421 cttcgcgata aatcacggtg gttcattctg ttcatgcctc cgcccgtccc ttgtaacgct
      481 ctacgcggga gacgacattg aatccgagcc gccgcagcgg ctccggcggc atccacgcgg
      541 ccctgccgaa gagcgcagcg atgtcgggtg cgccgaagcc gagagccacc tccgccagca
      601 gcctgccgaa aagcgttccc ttgacgacgc ctcccccgtt gcatcccgcg gcaacgaaca
      661 gtcccggcgc gtattctccc cacaccggcg ccccgttcag cgtgaagccg gtggcgccgg
      721 accagacgct tgagaagtcc agttcccgta gctgcggata gcgccgctcc agcgcgcttt
      781 tgagcatcac gcttaccgtt ccgctgtccg cttccgcctc gtaaccgtag agcgagcgaa
      841 tcagcaatcg tccatccccg gtgcgcctca atgtgctgcc cagccggtgc gccggcagca
      901 gtccccaggc gagatcgctg cccagcgagt cgaggctccc gggtgacagt ggtttcgtca
      961 tggcggcgta ggtgtacatg gcgacgatac gggaccccgc tatgccgagg ttcttgcaaa
     1021 aggcattgtt ggcgaggatc agcagcggcg catgcacgct tgccttcggt gtgcgtgccg
     1081 tccaggtgcc gcccttcctt tcgattgcga cgacgggaga gttctcgtgc aggaccaccc
     1141 gcggcggcag gctgtccgcc agtccgcgga tgagtgccgc gggctgtacc aggtagcagt
     1201 gaggggagta caggccttct ttgtaaaagc gcgtgcccag ccggtcgtgc agttgctgcc
     1261 ggtcgagggg ttcgtaagga aggcatgctg cgtccaggaa cgcgcggtag ttgcgcaatg
     1321 ccgcgatgcc cgctgcgccg gccgcggcgc gataggttcc tgtccgcgac aggtcgcagg
     1381 ctatttcgtt cgtgtccacg aggcgcttca aaccggtcat tgcgtcggca atcaggcgat
     1441 tgcaagccgt catccgttcc aactgccggg ggtccgcatc gtcggcgaga gcgatttcga
     1501 gcatgaagcc cgaattacgg ccggggctgc cttcaccgac ttcgctcgca tccagtacca
     1561 caatgcgcgc ctccggttcg agttcttgaa accgccgggc cgcggcaagg cctgtatagc
     1621 ctgcccctac gatcacgaca tcgcatcgca agtcctcttc gagctcagca tttacgcgtc
     1681 gtggcggcag caatgcgttc cagccgcagc gctgccgata gcgcgggaaa gatgccgctt
     1741 cagtcctgtt gctcacccga tgcgcgccgg gattggacgg ccgaagacgg gatacggaca
     1801 atagcgcctg ccgttcaaag gagccgatgg gtgatcaata cagatgtcgt gatcgttggc
     1861 gcaggtccct gcggcctgtt ccaggtgttc gagctcggcc tgctggggct gaaggcagag
     1921 gttgtcgatt cgatttcgaa accgggcggc caatgcgccg agctgtaccc ggacaagccg
     1981 atctacgata tcccggcgat cccggtatgc acgggggaag agttgacgaa cagcctcctg
     2041 aagcagattg agccgttcaa ctgcggcatg cacctgggcg aggaagtaat catcgtcagg
     2101 cgccaggagg acgatacatt tttcgtcgag accgatgccc gcaagcggtt tacggctcgt
     2161 gcggtggtga tagctggcgg cgtcggatcg ttccagcccc ggccattgcg cgtgccggat
     2221 gccgagctct acgaagatcg ttccctgcat taccgcgttc gcgacgcgtc gcagtttcat
     2281 gatcgcagga tcgtgatcct tggcggcggc gattccgcgc tcgactgggt gctggcgctg
     2341 gtcggaaaag caagtcacat cacgctcgtg caccggcgag acgaataccg ggcggcgccg
     2401 gcctcggtcg ggaaaatgcg gtcactggtt gccgaccggc ggatggacct gatcgagcat
     2461 ggtaaagctg ttgcaattac cggagccgag gggcgcatga gcgccataac cgtaaatgtc
     2521 agggccggcg attctgcgga agaacgctcg ctcgtcgtgc cggccgaaca cgcgctggtg
     2581 ttcttcgggt tgtcgccgaa actcggcccg atcgccgact ggggcctcga tatcaaccgc
     2641 aagaccatca acgttgatac agaacgtttc gagacatcgg agcctgggat ctacgccatc
     2701 ggggacatca accagtaccc gggcaagaaa aaactgatcc tgtgcggttt tcacgaagcg
     2761 gcgctcgcgg ccttcgccat caagcagcgc ctggagcccg acaagaaagt gcatgtgcaa
     2821 tacacgacca ccagcccgct gatgcacaag cgcctcggcc tgcctgattg acgcgtgaaa
     2881 ggggacagat ttttttgtca tgctggagaa agtagatgcg ctcatcgcga aaacattgat
     2941 ctgtcccttt ttaggttacc tcgactgcac gcccttgatc gcaccttcga tcaggttcag
     3001 ggctgcctgc cggctgacgc ccgccgggtc ccgcggtcct gcaggcgtcg aggcgatgcc
     3061 gagcgcgacc gtcttcgggt tcctgaacat gagggtcatc gccgctgcaa ggtcccggct
     3121 gctcgggccg tccgtcaccg cgagatcgtg tccaggtact tcctcgggat ccagcacgtc
     3181 catgtcgatg tgcacgtaga tggcatcgac gcggcttgcc agatcatcca tctggcggcg
     3241 taaggcgccc gatagtgacc ggacgtcctc tactgaaaat tgctgcacct cgtattcgcg
     3301 gaatctctcc gcttccagcg ggtcgaggtc gcgcacgccg ccccagacga tgtgctccat
     3361 cggcaacggt tccgtaaggc ccgacgtttt ccggatgttg tgcaacgcat gtcccgctgc
     3421 aacagctacg ggcatgccgc cgagcattcc ggacaaggtg gtttccggca cgttgaagtc
     3481 gccgtgcgcg tcgatgaaca ccagaccgac tctccgcgta tggccctcct cgtcgtactt
     3541 cagcccggcc agcattccga gcagcacgtt gcaatttgcc tcgaggccga tcgtcagcac
     3601 gtcatcgctg cgtccctccc gcaccagctt tgccatgttg gaatttgcga gcgccatgcg
     3661 gttccattcg ccgtactgcc gttcctgcgc cgcgtcgagt cgtacatcct ggacggggcg
     3721 gatcagctgg ccaccccagc tttcgatgag gcgctccagt ccggaagcgt ggatgtagtc
     3781 gggattcatc gatatctccg ccacgttgcg cgcgccgccg tagggctgct tgatgacgtc
     3841 gacttgcact gccggagccg atatggcgag agccggaagg acggacagca tcacggccga
     3901 gatcgcggtt aatataaagc gcatgccagg tttcctcaaa gtagaaaggg gacagtatta
     3961 tttttcttgc tgcaccggtt aaagtcatca tgctcatcgg gaacaagtag atccgtccca
     4021 taaaacggca aaaataaatc tgttccatta atccccgtcg ggacgttcga gttgcggata
     4081 atcgatgccc agttgctcga gataggtatc gtagcgcgac gggtcgtagt agaactcgcg
     4141 catccggttc cggtagctgt tcattgtttc ctcatttttc tcgatggcgg gaggatcgtc
     4201 gcgcgagatg aacggaacat actcgtcgcc cgcaagctgt tcctcgcgaa agtaccgcca
     4261 ggcctccctg cgtaattcgt cgtcctgcag caggtcaagc atggtggcgg cgatgacttt
     4321 cgccccggcg gtagcgccct tgtgcgcaat aggggttgcc atagccatcg cgctggacca
     4381 gtgatggaag gtcatcccct ccacgttcga gggaaacctg agcgtgacgg tggggacgtt
     4441 ccaggataca tcgccgatgt cgtccgagcc gccggatacc ggctcttccc ttggctcctc
     4501 gatgccgtcg agctcggcag cgagacctgt tatttcatcg gcgcccatca actcctggag
     4561 cgctttcgca aactgctggt cgtcctcgct ccactcaggc agccctatcg cccggatgtt
//
LOCUS       DASUOM010000532         5593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_184975,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000532 DASUOM010000000
VERSION     DASUOM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184975"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFY27_11075"
     CDS             complement(<1..570)
                     /locus_tag="VFY27_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5766101.1"
                     /translation="MLAEPFPLHLDWAGAPALPPAFIFFAGAVLLLLVGRRQRLQQAV
                     LLLVPVAGVLNLLTLEGGSSFVWEAMQFDLIQLRVDRLSILFGLLFHLGAFLGVIYSL
                     RVHERAQHVAALTYAGSALGAVFAGDLISLFFYWEGMAVSSAFLVWAGRTQESNGAGL
                     RYLGMHLLSGLLLLAGAVMHYRATGSIAFD"
     gene            complement(570..815)
                     /locus_tag="VFY27_11080"
     CDS             complement(570..815)
                     /locus_tag="VFY27_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766102.1"
                     /translation="MSRKAARIALTALHIAAFVAVVVELLWPFPDDAHAVERIHALDF
                     PASYAIYGFVSCVLLVLVGRGLRRLVMRDEDYYREDR"
     gene            complement(869..2365)
                     /locus_tag="VFY27_11085"
     CDS             complement(869..2365)
                     /locus_tag="VFY27_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5766103.1"
                     /translation="MLDTTSWPVAAALLVPVVGASGVLLLRHSPNLREAASLASAAAL
                     FFFVLSLVPAVANGARPGFTLLEFLPGINIAFRAEPLGTGFALVVAFLWFITVVYAIG
                     YMRVHEERNQSRFYAFFALAIAATVGAAFAANLMTLYLFYEALTLATYPLVTHGGAEK
                     DRAGGRTYLVILLTTSLTFLLLALAWTYAISGTLEFVEGGIYRDGDGLIGGMTPALLA
                     LLFTLFLFGTGKAALMPFHRWLPAAMVAPTPVSALLHAVAVVKLGVFAILKITVYTFG
                     LKTLSDFGVALVMQYVAAATLLIAAIYAIREDNLKRRLAYSTISQLAYIVLAASLANE
                     LSVMAGGMHILAHAFAKITLFFCAGMILVSLHKTTVTEMRGIGRTMPITLTAWVVASL
                     CVIGLPLTGGFWSKWYLAHGALAAGEPLMMGIILLGSLLAMAYLLPPAIRAFYTPPED
                     SAETGVVFQSEAPWPSLLALSITAIMTVLLFFVVQPVFELLSGFARQR"
     gene            complement(2368..2829)
                     /locus_tag="VFY27_11090"
     CDS             complement(2368..2829)
                     /locus_tag="VFY27_11090"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HEX5766104.1"
                     /translation="MIQRGPAVSLLLWLCLAPAPASAEEWLTGGELERSCELLLQDPA
                     RSNGLLCLAFVQGFIAGADTSVSAVGPRRTASSSEKETYADRAARTRLGTLRLQQIQS
                     TYGRYCIGDQVTAVEVVEKVTDYLESHPEASNVTAHDAVREALVYSFPCES"
     gene            complement(2923..3675)
                     /locus_tag="VFY27_11095"
     CDS             complement(2923..3675)
                     /locus_tag="VFY27_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766105.1"
                     /translation="MHKEIDLEFELHAYVDGDLDDEAMTRVEEYLVENPDTAAKVRAY
                     LQQKDELRRFARAQTSTDEPPALRDLGKKLARRLKPGHFFPWRLAVIMPLVFAAGWLG
                     HIFYIPLVQGPGYTNEVFQAHLLTSSDPSEILPLSPERMHKLFARIDEIERLPDLHQL
                     GFEPIGAQLLPTDDGVVLHIPYRNASGAVVSWFLSHDMQAAEEAPRLLHRQDVSIVTW
                     QHAQSRYAIAASLNDDELTRLAEYVDAHDDPY"
     gene            complement(3677..4180)
                     /locus_tag="VFY27_11100"
     CDS             complement(3677..4180)
                     /locus_tag="VFY27_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5766106.1"
                     /translation="MIDDLHAHISSLRRYAFVLCRNPADADDLVQESLVKAIAAADTY
                     QAGKDLRAWLFSILHNTFVSHKRQFARRARAARFLDATLLEAGVPPVQEKHVEAQHTL
                     NMLSRLTPEQQSVLVLIAVEGLSYAEAAEALDVPIGTLMSRLARGREELRRLVASSKG
                     RQLKAVR"
     gene            complement(4248..>5593)
                     /locus_tag="VFY27_11105"
     CDS             complement(4248..>5593)
                     /locus_tag="VFY27_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5766107.1"
                     /translation="DGDAHIYVKGAPERVLHMCRTQPCNGSQGPLDIEYWTGRIAEAA
                     AEGERLLAFAMEPEPTTVVDIDFHHVEEGLVFLGLVGFIDPPREEAIAAIGECHSAGI
                     AVKMITGDHGATAAAIARQLGLDENPEVMTGADLDRIGDDQLPDLVARTSVFARTSPE
                     HKLRIVRALQSRNEIVAMTGDGVNDAPSLKQADVGIAMGDKGTDAAKEAAQMVLLDDN
                     FASIVAAVHEGRVVYDNIRKVVAWTLPTNGGEALIIISAIAFGFTLPISAVQILWINL
                     VTAATLGLVLAFEPAEPDVMRRRPRAPDAGLLTPFLVWRVVLVSFLFLGVALAFYFHA
                     LGRGLDLATARTLVVNVIVVLEMFYLFNVRYLHMTSFSWRGVLGTPAVLIAIIVIVIA
                     QFAFTYLPVMQRLFDTRAVSLVDGVLVIAAGASVMVLLEIEKIFLRRSGRLTVEG"
BASE COUNT         1197 a         1709 c         1677 g         1010 t
ORIGIN      
        1 gtcgaaggcg atactccccg tggcgcggta atgcatcacg gcgcctgcca gcaggagcag
       61 tcccgacaac aggtgcattc cgaggtagcg aaggccggct ccattcgatt cctgggtgcg
      121 tccggcccag acgaggaagg ccgacgaaac cgccattcct tcccagtaga agaagaggct
      181 gatgaggtca ccggcaaaga ccgcgcccag tgccgagccg gcgtaggtga gcgctgcaac
      241 atgctgcgcc ctctcgtgaa cgcgcagcga atagatgacg ccaaggaagg ctccgaggtg
      301 aaacaggaga ccgaacagga tgctcaggcg atcaacgcgc agctggatga ggtcgaactg
      361 cattgcctcc cagacaaacg agcttccccc ctcaagcgtc aggaggttca gtacgcctgc
      421 aaccgggacc agcagcagca ccgcctgttg aagccgttgc ctgcgcccca ccagtagcaa
      481 cagaacagct ccggcaaaaa agataaaggc cggcggcaac gccggcgcgc ccgcccagtc
      541 aaggtgcaag gggaacggct cggccagcat cacctgtcct ccctgtaata gtcctcgtcc
      601 cgcatcacga ggcgccgcag tcccctgcct acgagcacga ggaggacgca ggagacgaac
      661 ccgtaaattg catacgaggc tggaaagtcg agagcgtgta tgcgctcaac tgcgtgtgcg
      721 tcgtccggga acggccacag gagttcgacg accaccgcga cgaatgccgc gatgtgcagc
      781 gcggttagcg cgatgcgagc ggctttccgg ctcacccaga aaagcggtcc gtcccgattt
      841 ccggctacac ctgatcgtcc gacttcgttc atcgttgtct cgcgaacccc gaaagcagct
      901 cgaaaaccgg ctgcaccaca aaaaacagta gtaccgtcat aattgctgtt atggacagcg
      961 ccaacaggga cggccacggg gcttccgatt gaaatacgac ccctgtctcc gcggagtcct
     1021 ccggcggcgt atagaacgca cggatcgccg gcggtaacag gtaagccatt gcaagcaggg
     1081 atccgagcag gatgatgccc atcatcagtg gttctccggc cgcaagtgca ccgtgcgcga
     1141 gataccactt gctccagaaa ccgccggtca gtggcagccc gatcacgcac agacttgcaa
     1201 ctacccacgc cgtcagggta attggcatgg ttcgcccgat gcctcgcatc tccgtgaccg
     1261 tcgtcttgtg caaagatacg aggatcattc ccgcgcagaa gaaaagcgtt attttggcga
     1321 atgcatgggc caggatgtgc atgccgcctg ccatcacgga cagctcgttg gcgaggctgg
     1381 cggcaagaac gatgtacgcg agttggctga tcgtcgagta ggcgaggcgc cgcttcaggt
     1441 tatcttcgcg aattgcatag atagcggcga tcagcagggt tgccgctgcg acatactgca
     1501 tgaccagagc cacgccaaag tcagaaagtg tcttaaggcc gaaggtgtag accgttatct
     1561 tgagtattgc gaacacgccg agcttgacta cggccaccgc gtgcagcagc gctgagacgg
     1621 gtgtcggggc aaccatcgct gcgggcagcc accggtgaaa cggcattagc gccgctttgc
     1681 cggtaccgaa caggaacaac gtgaagagaa gcgccagcaa ggcgggggtc attccgccga
     1741 tcagtccgtc accgtcgcga tatataccgc cttcgacgaa ctccagtgtt ccactgatcg
     1801 cgtaggtcca cgccagcgca agcagcagga acgtcagcga cgttgttaga agaatgacca
     1861 ggtaagtacg gccgccggca cgatctttct ctgcaccgcc gtgggtcacc aagggatagg
     1921 tcgcaagcgt caacgcctca tagaacaggt agagcgtcat gaggttggcg gcgaatgccg
     1981 cgccaacagt ggcagcgatg gctagcgcga agaaggcata gaagcgcgac tggttccgtt
     2041 cctcgtggac acgcatgtac ccgatcgcat ataccacggt gatgaaccag agaaacgcca
     2101 cgaccagcgc gaaacccgtg cccagcggtt ccgccctgaa ggcaatgttt atgccgggca
     2161 gaaactcgag gagggtaaat ccgggtctcg cgccgttcgc aaccgcaggc accagcgaca
     2221 ggacgaagaa gaaaagtgct gccgccgacg cgagcgacgc cgcttcccgc agattggggg
     2281 aatgccgtaa cagcagcacc ccagatgctc ccacgacagg cacgagaagg gctgccgcga
     2341 ccggccatga tgtggtatcg agcattgcta actttcgcag ggaaagctgt agaccagcgc
     2401 ttccctgacg gcatcgtgcg cggtgacgtt ggacgcttcg gggtggctct cgaggtaatc
     2461 cgtgaccttt tcgacaacct cgaccgccgt tacctggtcc ccgatgcaat agcgcccgta
     2521 cgttgactga atttgctgaa gtcgcagggt accgagacga gtgcgagcgg cgcgatccgc
     2581 ataggtttct ttttcagagg aggaagctgt acgtcggggg ccgacggcgc tgaccgaagt
     2641 atcggcgcca gctatgaagc cctggacgaa cgccaggcaa agcaagccat tcgagcgtgc
     2701 cggatcctga aggagcagct cgcaggaacg ctcgagttcg cctccggtaa gccactcctc
     2761 cgcggacgcc ggggcgggtg caaggcaaag ccacagcaaa agcgaaactg ccggtccacg
     2821 ctgaatcatg cgtccctcct gacaaacgga gcgtgccgcc gggaggacgg cgctcctatg
     2881 tgtcatgtac gcgggaggga gggttttagt ccaccgaagt tgtcaataag ggtcgtcgtg
     2941 ggcgtcaacg tattccgcca ggcgggtcag ttcgtcatca ttaagtgagg ctgcgattgc
     3001 gtaacgggat tgggcatgct gccatgtgac aatggagaca tcctgcctgt gcagcagccg
     3061 cggagcttct tccgccgctt gcatatcgtg cgacaggaac cacgaaacga cggcacccga
     3121 ggcgtttctg tagggtatgt ggagcaccac tccatcgtcg gtcggcagca gctgcgcgcc
     3181 gatgggctca aacccgagtt ggtgcaggtc cggcaatcgc tcgatctcgt cgatgcgtgc
     3241 aaacaactta tgcatgcgct caggcgagag cggcaagatc tcgctgggat ccgacgaagt
     3301 caaaagatgc gcctgaaaaa cctcgttcgt gtagccgggg ccctgcacca gcggaatata
     3361 gaaaatgtgg ccgagccatc cggccgcgaa taccagcggc atgatcactg caaggcgcca
     3421 gggaaaaaaa tgcccgggct tcaaacgtct tgcgagtttc ttgccgagat cgcgcaaggc
     3481 cgggggttcg tcggttgaag tctgggcgcg ggcaaagcgg cgaagctcgt ccttctgctg
     3541 caggtaagcg cgaacctttg ctgccgtgtc cggattctcg accagatact cctcgactcg
     3601 cgtcatcgct tcgtcgtcga ggtcgccatc gacataagcg tgcaactcga attccaggtc
     3661 gatttccttg tgcatcctat ctcaccgctt ttaattgcct gcccttgctg ctggcgacca
     3721 gccgccggag ttcttcccgg cctcgcgcga ggcgcgacat cagtgttcca atcgggacat
     3781 cgagcgcttc tgctgcctct gcataggata ggccttccac tgctatcagg accagcaccg
     3841 actgctgctc cggggtgagc cggctcagca tgttcagcgt gtgctgcgcc tcgacgtgtt
     3901 tctcctgtac cggcggcact cctgcctcga gcagcgttgc atcaagaaac ctggcggccc
     3961 gggcgcgccg tgcaaactgt cgcttgtgag agacgaaagt attgtggagg atgctgaaga
     4021 gccaggcgcg caggtccttc ccggcctggt atgtatctgc ggcagcaatg gctttcacca
     4081 gcgattcctg tacaaggtcg tctgcatccg cggggtttcg gcacagaaca aacgcgtacc
     4141 tgcgcaagct cgaaatgtgg gcgtgcaggt cgtcaatcat cgattctccc gtttacggat
     4201 atgtatacgc ccccggaagc gcctttagtc cgtacttacc ggctttttta cccctccact
     4261 gtcaaacgtc ccgaccgtcg caggaaaatc ttctcgatct ccagcaaaac cattacggac
     4321 gcgccggcgg cgatgacgag caccccgtcc accaggctca ccgcgcgagt atcgaacaat
     4381 cgctgcatca caggcaggta agtaaatgcg aactgcgcga taacgattac gattatggcg
     4441 atgagcacgg ccggggttcc gagtacgccc cgccagctga acgacgtcat gtggagataa
     4501 cggacgttga acaggtagaa catctccagc acaacgatga cgttgaccac cagcgtccgc
     4561 gcagttgcga ggtcgaggcc tcgtccaaga gcatggaagt aaaacgcaag cgcaacgccg
     4621 aggaaaagaa acgataccag caccacgcgc cataccagga acggcgtcag cagcccggcg
     4681 tccggcgccc tcggacggcg gcgcatgacg tccggctcgg ccggctcgaa cgccagcacc
     4741 agtcccaaag tcgccgctgt gaccaggttg atccatagaa tctgcactgc cgatatcggc
     4801 agcgtgaagc cgaaggcgat ggcgctgatg atgatgaggg cctcgccgcc gttcgttggc
     4861 aaggtccatg ccacgacctt gcggatgttg tcgtagacca cgcgaccttc gtgcacggcc
     4921 gcgacaatag acgcgaagtt gtcatccaac agcaccatct gggcggcttc cttggctgca
     4981 tccgtgcctt tgtcgcccat cgcgatacca acgtccgcct gcttcagcga cggcgcatcg
     5041 ttcaccccgt ctcctgtcat cgcgacgatt tcgttcctcg attgcaacgc ccgcacgatg
     5101 cgcagtttgt gttcgggact cgtcctcgcg aatacgcttg ttcgtgcaac gaggtccggc
     5161 agctggtcat ccccgatgcg gtccaggtcg gcgcccgtca taacttcggg attctcatcc
     5221 aaacccagct gacgggcgat cgccgcagcg gtggccccgt gatccccggt gatcatcttc
     5281 accgcgattc cggctgagtg gcattctccg atcgccgcga tcgcctcttc gcgcggtggg
     5341 tcgatgaagc ctaccaggcc gagaaatacc aggccttcct caacatggtg aaagtcgatg
     5401 tcgactaccg ttgtcggctc gggctccatg gcgaaagcaa gcagtcgctc tccttccgca
     5461 gctgcctctg caatgcggcc ggtccagtat tcgatatcca gcggcccctg gctcccgtta
     5521 caaggctgcg tccgacacat gtgaaggaca cgctctggcg cgcccttgac gtatatgtgg
     5581 gcgtccccgt cgg
//
LOCUS       DASUOM010000533         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1155231,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000533 DASUOM010000000
VERSION     DASUOM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155231"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFY27_11110"
     CDS             complement(<1..375)
                     /locus_tag="VFY27_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766108.1"
                     /translation="MQIISFAKMTAISALLALAACDGGSASFNPLPPDDPPPPDEPPA
                     IAVEPAFRQLAFEQPLAMMQAPGDSTSWFVVEKAGAVWVFPDDPDATLNDVALFADIS
                     DRVDSSPSEAGLLGMAFHPDYGT"
     gene            561..1862
                     /locus_tag="VFY27_11115"
     CDS             561..1862
                     /locus_tag="VFY27_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5766109.1"
                     /translation="MQRVITAFFCLAISSGALAQDAKRDLSDAFAAIEPRVIEWRRDF
                     HEHPELSNREFRTAEKVAAHLRSLDIDSVETGVAHTGVIGTLKGGKPGPVIALRADMD
                     ALPVKEQTGLPFASKAKGEFNGQEVDVMHACGHDTHTAILMGAAEILAANRDQLAGTV
                     KFFFQPAEEGAPVGEEGGAALMIKEGVLQGPDAPEAIFGMHAWPVPAGTINYRAGSFM
                     AASDSLYITVKGRQAHGSAPWMGVDPIFVSSQIVTALQGIPSRQLDITKGPAVITVGS
                     MHGGVRGNIIPDVVEMMGTIRTFDAGVREELHAKLKKTVNLVAEAAGAEATITIDPYS
                     PVTGNHPELLQQMMPTLAWAAGEDNVTEHPLITGAEDFAHFQQQIPGLYLMLGINKEG
                     VGAGQAPSNHSPLFFVNEDALIVGVRTMVGLALDYADRTAD"
     gene            1993..>2988
                     /locus_tag="VFY27_11120"
     CDS             1993..>2988
                     /locus_tag="VFY27_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5766110.1"
                     /translation="MRDQRNIAELVASQQPGRPLEQRFYTDPDVYQLEIDRILTQNWF
                     LAGHVSQLAAPGDFFVTRMAKESAIVVRGSDGKVKAFANVCRHRGSLVCLEQSGSVKK
                     FECPYHGWMYDIDGNLTAARSMPPDFDKRPYGLLPVSVEVLGGLVFIAFTDNPPSLRG
                     AKRDLAEPFEMFGFEDLKLAAKKSYPIAANWKLAIENYQECYHCATAHPDYARMHTLM
                     LDPAKRDRVQDKMLSRFAACGLRQIEFDFVDTLARPGEQGYGYSRTALFEGYKTGSRD
                     GEPLAPLLGNLKGYDGGASDFTVGPFSFLLAYSDHVVGYVFKPADHANCVCEIYWF"
BASE COUNT          593 a          937 c          901 g          557 t
ORIGIN      
        1 cgtaccgtag tccgggtgga aggccatgcc cagcagtccg gcttcgctcg gcgacgagtc
       61 gacgcggtcg ctgatatcgg cgaacagcgc aacgtcgttc agcgttgcat ccggatcatc
      121 cggaaacacc cacaccgccc ccgctttctc caccacgaac caactcgtgg aatcgccggg
      181 agcctgcatc atggcaagcg gttgctcgaa agcgagctgg cggaatgcgg gctcgaccgc
      241 gatagccggc ggctcatcgg gcggtggcgg gtcgtccggc ggcaacgggt tgaagcttgc
      301 ggagccgccg tcgcaggccg ctagcgcaag caaggcagaa atcgccgtca ttttcgcgaa
      361 gctgattatt tgcataactt tcttccagcg cgatgcgatg tgcctgtaga cagcataggc
      421 agtcaataag tgcctgtcac gatgtcgctg ccgcgcgggc ggaccgctac ccggtgctcc
      481 gtattatgtt attcccgcgc acgggatcgg cggcgggcgt aaggtacagt gtcatttgtc
      541 cagctccgga gtattgtttc atgcaacgcg tcatcaccgc cttcttctgt ctcgccattt
      601 cttcaggcgc ccttgcgcag gacgcgaagc gggatctcag cgacgcattc gccgccatcg
      661 agcccagggt catcgagtgg cggcgcgact tccacgagca tccggaactg tcgaaccgcg
      721 aattcaggac ggcggagaaa gtcgcggcac accttcgcag cctcgatatc gactccgtgg
      781 agaccggagt tgcacacacc ggtgtcatcg gcacactgaa gggcggcaag cctggcccgg
      841 tcatcgccct gcgcgccgac atggatgcgc ttcccgtgaa agaacagacg ggcttaccgt
      901 tcgcatcgaa ggcgaaaggg gaattcaacg ggcaggaagt cgacgtgatg cacgcctgcg
      961 gtcacgatac tcacaccgcc atcctcatgg gagccgcgga gattcttgcc gcgaatcgcg
     1021 accagcttgc cggtaccgtc aagttcttct tccagcctgc cgaggaagga gcccctgtgg
     1081 gggaagaagg cggcgctgcc ctcatgatca aggagggcgt gctccagggt cccgatgcac
     1141 cggaagccat cttcgggatg cacgcatggc cggttccggc agggaccatc aactatcgcg
     1201 ccggcagctt catggctgct tccgacagcc tctacataac cgtgaaaggc cgccaggcgc
     1261 acgggtctgc gccgtggatg ggcgtcgacc cgatcttcgt ctcgtcgcag atcgtcacgg
     1321 cattgcaggg aattcccagc cgccagctcg acattacaaa gggccccgcc gtcatcaccg
     1381 tcggcagcat gcacggcggc gtacgcggca acatcatccc ggacgtggtc gagatgatgg
     1441 gtacgatccg gacgttcgac gccggcgtgc gcgaggagct gcacgcaaaa ctcaagaaga
     1501 ccgttaacct cgttgccgaa gcggccgggg ccgaagcgac catcacgatt gatccctaca
     1561 gccccgttac cggcaatcat cccgaattgc tgcagcagat gatgccgacg ctcgcctggg
     1621 cagcgggtga agacaacgtc acggagcacc cgctcatcac cggggcggag gactttgcgc
     1681 acttccagca gcaaattccc ggcctgtacc tgatgctcgg gatcaacaag gagggcgtcg
     1741 gtgcgggaca ggctccatcc aaccactcgc ccctgttctt cgttaatgaa gacgcgctga
     1801 tcgtcggggt acgaaccatg gtcgggctgg cactggacta tgccgacagg accgccgatt
     1861 agtctcggat agtcgccctc cggaagtttc agatgatgcg aggcgtgatt ccgcccaaat
     1921 gttcctgtgt tatcgtgctt tccatatagc ctgttaccac ccgtgacagg cgcataaacc
     1981 aggaacagca tcatgcggga ccagcgaaac atagcggagc tcgttgccag ccagcagccc
     2041 gggaggccgc tggagcagcg tttctacacc gatcccgatg tctaccagct cgagatcgac
     2101 aggatcctca cgcagaactg gtttctcgcc ggccatgtct cccagctggc cgctcccggc
     2161 gatttcttcg tgacccggat ggcaaaggaa tcggcgatcg tggtgcgcgg ctccgacggg
     2221 aaagtgaaag ccttcgccaa cgtttgccgt caccgcggct cgctggtctg cctggagcag
     2281 agcgggtccg tgaagaagtt cgaatgtccc taccacggct ggatgtacga cattgacggc
     2341 aacctcaccg ccgctcgctc catgccgccg gattttgaca agaggccgta cgggcttttg
     2401 cccgtctcgg tagaggtgtt aggcgggctc gtcttcatcg cgttcacgga caatccgccg
     2461 tcactgcggg gcgcgaaacg cgatcttgct gaaccgttcg aaatgttcgg cttcgaagac
     2521 ctcaagctcg cggcgaagaa gagctacccg atcgccgcga actggaaact cgctatcgag
     2581 aattaccagg agtgctatca ctgcgccacg gcccatcccg attatgcgcg catgcacacc
     2641 ctgatgctcg atcccgcgaa acgagaccgc gtccaggaca agatgctgag ccgttttgca
     2701 gcctgcggcc tgcggcaaat cgaattcgat tttgtcgata cgctggcgcg gccgggcgaa
     2761 cagggttacg gctacagccg caccgccctt ttcgaaggct acaagaccgg cagtcgcgat
     2821 ggcgaaccgc tagccccctt gctcggcaac ctgaaaggtt acgacggcgg tgcttccgac
     2881 tttacggtcg gaccgttctc gttcctgctg gcatacagcg accatgtggt gggttacgta
     2941 ttcaaacccg ccgatcatgc gaactgcgtc tgcgaaatct actggttc
//
LOCUS       DASUOM010000534         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_913366,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000534 DASUOM010000000
VERSION     DASUOM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913366"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFY27_11125"
     CDS             complement(<1..764)
                     /locus_tag="VFY27_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766111.1"
                     /translation="MPQAVPHADEDVALLEIHNATIYRGSTRVFNHFSLEIGQHEQVA
                     ILGPNGSGKTTLLKLINRELYPVLAADSWVRILGRSNWNVWELRSRIGIVSQDLQARY
                     YNTADGLEVVLSGYVSSIGLKGMPAGTVSDEQKSAGLRIMADLGVAHLADTPLRQMST
                     GEQRRCLLGRALVHQPDALILDEPTAGLDMAASFDYLGRIRQFVAEGRSIVLVTHLLN
                     EIPPEVERVILIREGVIIADGPKDQVLTIENLEESYG"
     gene            complement(768..1301)
                     /locus_tag="VFY27_11130"
     CDS             complement(768..1301)
                     /locus_tag="VFY27_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766112.1"
                     /translation="MQDLTLIANMAEIIGVIIVIGGLLFAMLQTRQFRQQRREMAAIE
                     LLRFFGSPRFAEAYRTILQMPEGMTAADIRGGAPELEKSAMLIGTTMENIGVMTYQRI
                     VPFMVVNNLVGSSTVILWRKLGNWVDGLREEMGEPYAFEWFQWLAERLADDHGDDPRP
                     AYEAHKDWKPARLTPEL"
     gene            complement(1347..2768)
                     /locus_tag="VFY27_11135"
     CDS             complement(1347..2768)
                     /locus_tag="VFY27_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5766113.1"
                     /translation="MSYDVPLKPASLPKRLRNFIREAISDNETDFTVGPIGRALGLLA
                     IPMMLEMSMEAVFALADIIFVSRLGTDAVAAVGLTEALVTVLYAVAIGLGMGVTAMIS
                     RRIGAKDNDGAARVAGQAIWIGAIVAAIIAFVGFNFAADLLGLMGASPGVIETGQGYT
                     TVILGGSASIIYLFLLNAAFRGAGDAPVALRSLTLANLINIVLDPCLIFGLGPFPELG
                     VTGAAIATTVGRGIGVLYLLYYLFAAKGRLTFRLRHAALDWSLIRRLISISIGGVGQF
                     LIATASWILIMRIVALYGSAPVAAYTIALRLMEFALLPAWGLGNAAATLVGQNLGARK
                     PDRAAESVWRAARYNATFLLVIGLVLIAFAPLIVSLFSTDPEIIRYGSDCLRIMGLGY
                     PMYAVGMIIIQALNGAGDTRTPSVLNFICFWLVQIPLAYALATAADLGPHGVFWSIVI
                     SESMLTFIGVAIFRKGRWKLQQV"
     gene            2885..3802
                     /locus_tag="VFY27_11140"
     CDS             2885..3802
                     /locus_tag="VFY27_11140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5766114.1"
                     /translation="MCRETGIRATIVIGMLGILGALAACDPGSRDEASKDGDARQNAD
                     RMLREHDGDSGTPSAGAGIPPDREVVAETLAYAEVNEELVYGHFVFPADMVNPLPGLI
                     VVHEWWGLNDGVRAMADRLAAEGYIVLAVDLFGGKTADHPPAARELMLAALQKPELAE
                     ANLRQAYEFIRDTGGAPKIGSLGWCFGGAWALNAALMFPDELDATVIYYGQVPAEAER
                     LAPLNVPILGLFAENDRGIPVETVRSFEQALENLEKDFEIEVFPDVGHAFANPSGTSY
                     DADAAGRAWELTTRFLKEHLGTTGDPAET"
     gene            complement(3829..>4175)
                     /locus_tag="VFY27_11145"
     CDS             complement(3829..>4175)
                     /locus_tag="VFY27_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766115.1"
                     /translation="NIADSRISRTGKDLENQVNQQLDITVPARQLAPLTLRDFCVIDP
                     VGEGSTTAAEKPVLPRQVAGSSRLSLVAALAHASLRCTRDDEHKTVYVSQPLDVTLIC
                     EASDAEGAVTAR"
BASE COUNT          828 a         1268 c         1310 g          769 t
ORIGIN      
        1 ccataggact cctccaggtt ttcgatcgtc aggacctggt ccttcgggcc atcggcgatg
       61 atgacgccct cacgaatcag tatgacgcgc tcgacctccg gcggaatttc gttcagcagg
      121 tgcgtgacga gcacgatcga acgtccctcg gcgacgaatt gcctgatccg ccccagatag
      181 tcgaagctcg cagccatgtc cagtccagcg gtgggttcat ccaggatcag cgcatctggc
      241 tggtggacga gtgcacggcc gagcaggcag cgtctctgct cccccgtcga catctgccga
      301 agcggcgtat cggcaaggtg agcgacaccg agatccgcca tgattcgcag tcctgcagat
      361 ttctgctcgt cgcttactgt tccggccggc atccccttga gcccgatgct cgagacatag
      421 ccggagagga cgacctccag gccgtcagcc gtattgtaat aacgtgcctg aaggtcctgg
      481 gagacgatgc cgatgcggga ccgcagttcc cacacgttcc agttgcttcg cccgaggatc
      541 ctcacccacg aatccgctgc cagaaccggg taaagttcgc ggttgatcag cttgagcagg
      601 gtggtcttgc cggacccgtt cggtccgagg atcgccacct gttcgtgctg tccgatctcg
      661 agactgaaat ggttgaatac ccgcgtgctg ccgcgataga tcgtcgcgtt gtgaatctcg
      721 agcagcgcta cgtcttcgtc cgcgtgcgga acggcctgcg gcacgatcta aagctcgggc
      781 gtcagtcgtg ccggtttcca gtccttgtgt gcctcgtatg ccggccttgg atcatcgcca
      841 tgatcgtctg caagccgctc ggccagccac tggaaccact cgaaggcgta cggctctccc
      901 atttcctccc gcagtccgtc cacccagttt cccagcttgc gccagaggat gacggtgctc
      961 gagccgacca ggttgttgac gaccatgaac ggcacgattc tctggtaggt catcacgccg
     1021 atgttttcca tcgtggtgcc gatcagcatc gcggattttt ccagctccgg cgcgccgccg
     1081 cggatatcgg cggcggtcat cccctccggc atctggagaa tagtgcggta ggcctcggca
     1141 aatctcgggc tgccgaagaa gcggagcagt tcaattgccg ccatttcgcg ccgctgctgc
     1201 ctgaactgcc gcgtctgcag catcgcaaac agcaaacctc cgatcacgat gatgacgcca
     1261 atgatctcgg ccatgttcgc gatcaatgtc aagtcttgca tgagtttttt cattccttgt
     1321 tctgaagcca gccgaatagt actccttcac acctgctgca gcttccacct tcccttgcgg
     1381 aatatcgcca ctccgatgaa ggtgagcatc gactccgata tgacgatgga ccagaaaacg
     1441 ccatgcgggc ccaggtccgc ggcagttgcc agggcgtagg ccagcgggat ctggaccagc
     1501 cagaaacata tgaagttcag gacggatggg gttcgcgtat cgccggcacc gttcagtgcc
     1561 tggatgatga tcatgccgac ggcgtacata ggatagccaa gtcccataat ccgaaggcaa
     1621 tcgctgccat acctgattat ttcggggtcg gtggagaaga ggctcacgat aagcggcgcg
     1681 aacgcgatca acacaaggcc gatcaccagc aagaaggtcg cattatagcg agccgctcgc
     1741 cagaccgact cggcggcgcg gtccggcttg cgcgccccga ggttttggcc caccagcgtt
     1801 gccgctgcgt tgccgagacc ccacgccggc agaagggcga attccataag cctcaaggcg
     1861 atcgtgtacg cagcaaccgg cgcactgccg tagagggcaa cgatgcgcat gatcaggatc
     1921 cagctggcgg tcgcgatcag gaactgcccg acgcccccga tggagatgct gatcagccgc
     1981 cggatgagcg accagtcgag cgcagcatga cgcaggcgga aagtcagccg gccctttgcc
     2041 gcgaagaggt aataaaggag gtacaggacg ccgatgccgc gtccgacggt ggtcgcgata
     2101 gccgcgccgg tcacgccgag ctccgggaat gggccaaggc cgaaaatcag gcagggatcg
     2161 agcacgatgt ttatcaggtt cgcaagcgtc agcgaacgca gtgcgaccgg ggcatccccg
     2221 gcgccgcgaa aggcggcgtt gagcaggaag aggtaaatga tgctggcgga gccgccgaga
     2281 ataaccgtgg tgtagccctg cccggtctcg atgacaccag ggctcgctcc catcagtccc
     2341 agcagatcgg cggcgaagtt gaatccgacg aaggcgatga ttgccgccac gatcgcgccg
     2401 atccagatag cctgtcccgc gacccgggcc gcaccgtcat tatcctttgc gccgatccgg
     2461 cgggaaatca tcgccgtaac gcccataccg agtccgatag cgacggcata aagaactgta
     2521 acaagtgctt ccgtcaggcc tacggcggcg acggcgtcgg tgcctagcct ggacacgaag
     2581 atgatgtccg ccagggcgaa gaccgcttcc atggacattt ccagcatcat cgggatcgcg
     2641 agcaggccga gggcgcgtcc tatcggtccg actgtgaaat cggtctcgtt gtcgctgatg
     2701 gcctcgcgaa taaaatttcg cagtcgcttc ggcagcgacg caggctttag cgggacgtca
     2761 taactcatgc agtggttttc caatgattac tgctgcgatc gcgaaacgct gcatgggcgc
     2821 gagtgcggat ctcagttgtt ttgctttaca ctcgacgctt ttggcgggtc gacgaggata
     2881 tacgatgtgc agggaaaccg gcatacgcgc gacgatagtc atcggcatgc tgggcatact
     2941 gggcgcgctc gccgcgtgtg acccgggatc tcgcgacgag gcatcaaagg atggggacgc
     3001 gcgacagaat gccgaccgga tgttgcgcga gcatgacgga gattccggaa cgccaagtgc
     3061 aggagccggc attccgcccg accgggaagt agtcgccgaa acgctggcat acgcggaagt
     3121 aaacgaagag ctggtctacg gccacttcgt atttcccgcg gacatggtca acccgcttcc
     3181 cggcctgatc gtcgtgcacg aatggtgggg cttgaacgac ggcgtgcggg cgatggctga
     3241 ccggctcgca gccgagggct acattgtgct cgcagtcgac ctgttcggcg gcaagacggc
     3301 cgaccatccc ccggctgccc gcgaactgat gcttgcagcg ttacagaaac cggagctggc
     3361 ggaagcaaac ctgcggcagg cgtacgagtt catcagggac accggcggcg caccgaaaat
     3421 cggctccctc ggatggtgct tcggcggcgc ctgggccctg aacgccgccc ttatgtttcc
     3481 ggatgagctg gacgcgacgg tcatttacta cggtcaggtt cctgccgagg cggagagact
     3541 ggcgccgctc aatgtgccga tactggggct gttcgccgag aacgaccgcg gcataccggt
     3601 ggaaaccgtc cggagcttcg agcaggcact ggaaaacctg gaaaaggact tcgagatcga
     3661 ggtcttcccg gacgtcggcc acgccttcgc gaacccgagc ggcacgagct acgacgctga
     3721 cgcggccggg cgtgcgtggg agctgacgac gcgattcctg aaggaacatt tggggacgac
     3781 cggagacccg gcggagacat agtccgcgtg ctacccgggg cttcggcgtc agcgtgcggt
     3841 gacggcacct tccgcgtcgg acgcctcgca gatcagcgtg acatccagag gttgtgatac
     3901 gtacaccgtc ttgtgctcat cgtccctggt acagcgcaag gaagcgtggg ccagggcggc
     3961 aaccaggctg aggcggctgg aaccggccac ctggcgcggc aagacaggct tttccgcggc
     4021 agtcgtggaa ccttcgccca ccgggtcgat cacacagaaa tcccgcagcg tgagaggcgc
     4081 cagctggcgg gcaggaactg tgatgtcaag ctgctgattt acctggtttt ccagatcctt
     4141 gccggtcctc gagatcctgc tgtcggcgat gttga
//
LOCUS       DASUOM010000535         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_311245,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000535 DASUOM010000000
VERSION     DASUOM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311245"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFY27_11150"
     CDS             complement(<1..417)
                     /locus_tag="VFY27_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5766116.1"
                     /translation="MRGVILALLFGGTLAAGGAAAEAVVIGSKNFTENYILAEAAAQL
                     LESKGIEVERRLGLNGTRISFEALVNGAIDVYPEYSGTISEVILGDPGLREWQDVAAA
                     IAERHLVLLEPLGFDNTYAIAVTGELAREHQLREISD"
     gene            complement(419..1183)
                     /locus_tag="VFY27_11155"
     CDS             complement(419..1183)
                     /locus_tag="VFY27_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766117.1"
                     /translation="MSTTHAPNAAEFNGVSLRRGAVLALDDVTLAMPLGQTTAVLGAS
                     GSGKSTLVQLIIGLLRPDTGTVRTLGEPIDFANPRPLRKRIGYAIQDVSLFPHLDVRA
                     NILLPATLSGWSEAEKGVRLDELLQLMHLPRDVLDRYPHELSGGQQQRAGLCRAMMLR
                     PELLLLDEPFSGLDTMTRKSIHEQFLHMQQQAPVSTVLVTHDPQEAINLSHGLVVMKS
                     GRVQQYGPVAEVIADPANEYVRHLCTALESLPVSTS"
     gene            1641..1970
                     /locus_tag="VFY27_11160"
                     /pseudo
     CDS             1641..1970
                     /locus_tag="VFY27_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            complement(2006..>2803)
                     /locus_tag="VFY27_11165"
     CDS             complement(2006..>2803)
                     /locus_tag="VFY27_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766118.1"
                     /translation="VARRDELQRQIKDIRGDLESVIRQRDQLQTEIKGRESRGETTVH
                     ALREKDNKIFKLSRELTSWQNRLPPLITRFRERDQEAKSLARKLGEAEQRIAQLESRQ
                     PVEHTRIEPAGAGALTEGLTACNDQFDESAEVDLSDLEDQIGDEDLASPVPVGTFDDT
                     ASYASYAEPSARPRGSSGRPGMPVNGVPALQVVKDDLQRIKGVGPAIERTLHDLGIFS
                     FDQIARISEYDINRIAERLKGFRSRIYREDWIGQARTLHDLQRGDPA"
BASE COUNT          488 a          885 c          875 g          555 t
ORIGIN      
        1 atccgaaatt tcccgcagct ggtgctcgcg tgccagttcg cccgtgaccg cgatcgcgta
       61 agtattgtcg aagccgagcg gctcgaggag gaccaggtgg cgttccgcga tagcagcggc
      121 gacatcctgc cattcccgta agccaggatc gccgaggatg acttccgata tcgtcccgct
      181 gtattccgga tatacgtcga tagcgccgtt taccagcgcc tcgaagctga tcctggtgcc
      241 gttcaggccc agccgccgct ccacctcgat acccttgctc tcgaggagct gggcggcggc
      301 ttccgccaga atgtagttct cggtgaagtt cttgctgccg atgaccacgg cttccgccgc
      361 cgcgccgcca gcggcaaggg tgccaccgaa gagcagtgcc aggatcacgc cacgcatgtc
      421 agctcgtcga caccggaagg ctttccagcg cggtgcaaag atgccttacg tattcgttcg
      481 cgggatcggc gataacttcg gcgactggcc cgtactgctg cacccgcccc gatttcatta
      541 cgacaaggcc atgagacagg ttgatcgctt cctgcggatc gtgggttaca aggacggtgg
      601 aaaccggcgc ctgctgctgc atgtgcagga actgctcgtg gatgctcttg cgcgtcatgg
      661 tatcgaggcc tgaaaacggc tcatcgagca gcagcagttc aggacgcagc atcatcgcgc
      721 gacagagccc cgcccgttgc tgttgaccac cggacaactc atgcgggtaa cggtcaagga
      781 catcgcgcgg caggtgcatc agctgcaata attcgtcgag gcgaacgcct ttctctgctt
      841 cgctccagcc ggacaacgtg gccggaagca agatgttcgc gcgcacgtcg aggtgcggaa
      901 acaacgagac gtcctggatc gcgtagccga tccgcttgcg cagcggtcgg gggtttgcga
      961 aatcgatagg ctcgcccagc gttcggaccg ttcccgtatc cggtcgcagc aagccgatga
     1021 tcagttgcac gagcgtcgat ttcccgctgc cgctcgcgcc aagcactgcc gtggtctgcc
     1081 ccagtggcat cgcaagcgtt acgtcgtcca atgcgagcac ggcgccccgt cgcaacgata
     1141 cgccattgaa ttcggcagca ttgggtgcgt gggtggtgct cacgtttgcg tcgcctgctt
     1201 ccgttccgtc cgagttcttg tatccgtccc tgcggcgcaa ggacaccaga gcaaagcaac
     1261 cttacgggcc acgccgccaa atggccagtc gaggcctcgt cgatcgtgca cgcgcggccg
     1321 gatgcaggcg cccgctctgc aacgtccaac ctgttaaact gcgaatgccc tcccggtact
     1381 cgctatcgga catccggacc ttttccgaag ccattcaatc cttcacatca aaaggcactc
     1441 gtcacatgaa atcatcgcga cccccttgcc tgttacctgt ccttctcgca ctggccgctt
     1501 gcagcccggg cgaagagggc gccggaatgc aggaacagca gcaggcgcag ccacaacagg
     1561 cggagccgca gcaggcggag cctgccgccg gcagcgaagt tgcaacgggt gagccggtcg
     1621 agctggagcc gggcttgacg atgcgggttc tgaaagaagg cagcggagag gtcgccgagc
     1681 ccgggcagac ggccgtcgtc cattacaccg gctggctgca cgacccgcag gcacccgaca
     1741 accgcgggga gaaattcgac agttccctgg atcgcggaca gcacttccag tttcctctgg
     1801 gcgccggggc agtcatagcg ggctgggatc ggggcgtcgc agggatgcgg gtcggggaag
     1861 tgagggagct cgagatcgcg cctgaactcg cctacggcga tcgcggcgcg ggtggcggcg
     1921 tgatcccgcc gggtgcgact ctggtgttcg aagttgaact tgccgacgtg gtgggcgcag
     1981 cggaataacg ccctgaccag gctccctagg cgggatctcc ccgttgaagg tcgtgcaggg
     2041 tacgcgcctg cccgatccag tcttcgcggt agattcgact gcggaaccct ttcagacgct
     2101 cagcaatcct gttgatgtcg tattcgctaa ttcttgcgat ctggtcgaag ctgaaaatgc
     2161 cgagatcgtg caaggttcgt tcgatggcgg gaccgacgcc cttgattcgt tgcaggtcgt
     2221 ccttgaccac ctgcagcgcc ggaaccccgt tcaccggcat tccggggcgg ccgctcgatc
     2281 cccgaggtcg cgcggacggc tcagcgtagc tcgcataact ggccgtatcg tcgaaagttc
     2341 ccacaggtac cggggaggcc aggtcttcgt cgccgatctg gtcctcgagg tcgcttaagt
     2401 ccacctccgc ggattcatcg aactggtcgt tgcacgcggt gagcccttcg gtaagtgctc
     2461 ccgcgcctgc gggttcgatg cgtgtgtgtt ctaccggctg ccggctttcg agttgggcga
     2521 tacgctgctc ggcttcaccg agcttccggg cgagtgattt cgcttcctgg tcccgctcgc
     2581 ggaatctcgt gatcagcgga ggcagccggt tttgccagct cgtcagctcc cggctcagct
     2641 tgaagatctt gttgtccttc tcgcgcaggg catgaacggt tgtttcgccc cgcgattcgc
     2701 gacccttgat ttctgtctgc aactggtcgc gctgcctgat aacgctctcc aggtcgccgc
     2761 gaatgtcttt gatctgccgc tgaagttcat cccggcgggc aac
//
LOCUS       DASUOM010000536         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_479791,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000536 DASUOM010000000
VERSION     DASUOM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479791"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFY27_11170"
     CDS             complement(<1..594)
                     /locus_tag="VFY27_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5766119.1"
                     /translation="MPRIDSFLKLGLAQGCSDVHLAVGVPPMLRMHGDLMPIKFRDLS
                     DTELESYVMEILTDSQKQHFDGGNDLDFSYVGEEGGRFRVNLFRKDTGVGAVFRHIPN
                     EVPTLEKLKVPPVIRDFCDYHQGMVLVTGSTGTGKSTTLAAMIDHLNKTRSLNIISLE
                     DPIEFVHPSKNCQVIQRELGTHLTSFADGVRSAMREDP"
     gene            complement(787..3168)
                     /locus_tag="VFY27_11175"
     CDS             complement(787..3168)
                     /locus_tag="VFY27_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5766120.1"
                     /translation="MAILAGFRADQLITQLIGEPDTASSNALKLTERLKKLGPKVIPK
                     AIDALAMCDRSHTMLLVDILASQVSDKTLKYYKEGLADGGDRVVSGTAWALSSSNSYN
                     ANALLDFLDDDEISKPAVIEILRVHKQGLSVHDLLRRAYELESKEKAALFKIIEEIVS
                     EDMVPDLIARMGGKDPAVKIHLIKLLSKFTKEEINRALEMQLGDTNKLVRSAALSALA
                     ERGGNVNIAKVSKLLHDPDLDVQNKAVDVMVRINHPETIRFLVPALKDESEYTRRAAV
                     EVLNEIGNADSVKDLLEAIKDDDWWVRARAGDALAEIGGPRVVDSVLQLIRDKDEDIR
                     RTAIEILNSTKDERAVDELMKATDDSDWWVRERAVDALSQIGSAKAVPKLLRMLGKNP
                     KTDTVVIRALGKLGNHEIVDKLVPLLQRPEREVQVEAIKAISHLADQKRADTIRKVLQ
                     KIKQSGDSTIINSADIAIKELDAKFSQTVLEENIRAEKIAEHTRTLLIENEDIDALLT
                     TPEDKVLDISNLEPGDVIDGRYKYIEKIGKGAFGTVLLMQDQVVDERLILKFLNPNVS
                     SDEEMMKRFVHELRYSRKITHRNVIRIYDFLHLQGAYAISMEYFPSHTLSGEIPDSKP
                     MAFDKALKYSFDIATGMAVAHQAGVIHRDLKPANILVNDEGLLKIVDFGVAAAASSGD
                     TQLTKTGYVIGSPKYMAPEQILGKKVDETADIYSVGVIMYEMVTGVPPYSRGDHMSVM
                     YQHVQGKAKRCQELNPNIPDDYAAVIARAMSVDKTKRYNSMDSLIEALSAVKL"
     gene            3575..4633
                     /gene="apbC"
                     /locus_tag="VFY27_11180"
     CDS             3575..4633
                     /gene="apbC"
                     /locus_tag="VFY27_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX5766121.1"
                     /translation="METSKVPGTEHLLRDVGRVKRASVEDGRARIEIELGFPAETAQV
                     ELRKFFQDLAKTASGAEDVEVVLSSKIASHGVQPNLKPLANVSNIIAIASGKGGVGKS
                     TTAANIALALSRDGASVGVLDADIYGPSQPLMLGLSGQRPTSADGKTMIPLQAHGLQV
                     MSIGFLIDADQPMVWRGPMVTQALNQLLQQTQWTDLDYLIVDMPPGTGDIQLTLSQRV
                     PVSGAVIVTTPQDIALLDARKGLQMFRKVSVPVLGIVENMSTHVCSNCGFEEPIFGSG
                     GGERMARDFDVQLLGQLPLDLQIREQTDSGTPTVVAEPDSPPAQAYMQTARRMAARLA
                     MQGKDYSRKFPNIVVEDS"
     gene            4703..>4903
                     /gene="dcd"
                     /locus_tag="VFY27_11185"
     CDS             4703..>4903
                     /gene="dcd"
                     /locus_tag="VFY27_11185"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876281.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5766122.1"
                     /translation="MSIKSDRWIRRMAVEHGMIDPFEPAQVRSNSGGRIVSYGTSSYG
                     YDVRCSDEFKIFTNIESVIVDPK"
BASE COUNT          892 a         1464 c         1502 g         1045 t
ORIGIN      
        1 gggatcctcg cgcattgccg agcgtacgcc atccgcgaaa ctggtgaggt gcgtgccgag
       61 ttcgcgctgg atgacttggc agttcttgct cggatgcacg aactcgatag ggtcttccag
      121 gctgatgata ttgaggctgc gcgtcttgtt caggtggtcg atcatggcgg caagcgtcgt
      181 cgacttgccg gtgccggtcg acccggtgac cagcaccatg ccctggtggt aatcgcaaaa
      241 atcgcggatg acgggcggca ccttcagttt ctcgagcgtc ggcacttcgt tcgggatgtg
      301 gcgaaatacc gccccgacgc cggtgtcctt gcggaacagg ttgacgcgga accggccacc
      361 ttcctctccg acgtaggaaa aatcgaggtc attgccgcca tcaaagtgct gcttctgcga
      421 gtccgtcagg atttccatga cgtagctttc gagctccgtg tccgacaggt cgcggaactt
      481 gatcggcatc aggtccccgt gcatgcgcag catcggcggc acgccgacgg ccagatggac
      541 gtccgagcag ccctgtgcga gccccagttt caggaacgaa tcgatacgtg gcatgaaatg
      601 tcttttcttt tctgaagcgc cgaacgctcc accccggtta tgtccgattg acgcaaaatt
      661 ccgccgaata ggcaagctcg cgggacacgg ttcgttaaat cgagcacaga cccgccggcc
      721 atgccgaagc gcgatgttct tcaccaaatc cgccgccggc ataaggacag ccaagcgtgc
      781 gtggtattac agcttcaccg cactcaacgc ctcgatgagc gagtccatcg agttgtagcg
      841 cttggtcttg tcgaccgaca tggccctggc aatgacggca gcgtagtcgt ccggaatgtt
      901 ggggttcagc tcctggcaac gcttcgcctt gccctggacg tgctggtaca tgactgacat
      961 gtgatcgccg cggctgtaag ggggaacgcc ggtgaccatt tcgtacatga ttacgccgac
     1021 gctgtaaatg tcggcggtct cgtcgacctt cttgcccagg atctgttccg gtgccatgta
     1081 cttcggggaa ccgatgacat agccggtctt ggtgagctgc gtatccccgc tgctggcggc
     1141 tgccgcaacg ccgaagtcca cgatcttgag cagcccctcg tcgttgacga ggatgttggc
     1201 cggcttcagg tcgcggtgga tgacgccggc ctggtgcgcg accgccatgc cggtggcgat
     1261 gtcgaaggaa tatttcagcg ccttgtcgaa tgccatcggc ttgctgtcgg gtatttcgcc
     1321 actcaaggtg tgtgacggaa aatattccat ggagatggca taagctccct gcaggtgcag
     1381 gaagtcgtaa atgcggatga cgttgcggtg cgtgatcttg cgcgagtagc gcaactcgtg
     1441 cacgaagcgt ttcatcatct cttcgtcgga cgagacgttc gggttcaaga acttgaggat
     1501 cagccgctcg tccaccacct ggtcctgcat cagcagcacg gtaccgaaag cgcccttgcc
     1561 gatcttctcg atgtacttgt agcgaccgtc gatgacgtcc cccggctcca ggttggagat
     1621 gtcgagaacc ttgtcttcgg gtgtcgttag cagtgcgtcg atgtcttcgt tttcgatcag
     1681 cagcgtgcgc gtgtgctccg cgatcttttc cgcgcggatg ttctcctcca gcacggtctg
     1741 cgagaatttg gcatcgagct cctttatggc gatgtcggcg ctgttgatga tcgtcgaatc
     1801 tccggactgc ttgatcttct gcaacacctt gcgaatcgta tccgcgcgct tctggtcggc
     1861 gaggtgcgaa atagccttga tcgcctccac ctggacctcg cgttccggac gctgcaacag
     1921 cggaaccagc ttgtcgacaa tctcgtgatt gccgagtttg cccagtgccc ggatgacgac
     1981 ggtgtcggtc ttggggttct tgccgagcat gcgcagcaac ttcggcactg ctttggcgct
     2041 gccgatctgc gagagcgcgt cgaccgcccg ttcgcgaacc caccagtcac tgtcgtcggt
     2101 ggccttcatc aactcgtcga ccgcgcgctc gtccttggtg gagttgagga tttcaatggc
     2161 ggtgcggcgg atgtcctcat ctttgtcccg gatgagctgc agcaccgagt cgactacccg
     2221 cggcccaccg atctcggcga gtgcgtcgcc cgctcgggcc cgcacccacc agtcgtcgtc
     2281 cttgatcgcc tccagcagat cctttaccga gtccgcgttg ccgatttcat tcagcacctc
     2341 gacggcggca cgccgggtgt actcggattc gtccttcagc gccggtacca ggaagcgaat
     2401 ggtttccggg tgattgatcc tgaccattac gtcgacagcc ttgttctgga catcgaggtc
     2461 agggtcatgc aggagtttgc tgaccttcgc gatgttcacg ttgccgccgc gttccgcgag
     2521 ggcgctcagt gcggcgctgc gaaccagctt gttcgtatcg ccgagctgca tctcgagcgc
     2581 ccggttgatc tcctctttcg tgaacttcga cagcagcttg atcaggtgga ttttgaccgc
     2641 cggatccttg ccccccatgc gagcgatcag gtcgggcacc atgtcctccg acacgatctc
     2701 ctcgatgatc ttgaatagcg cggctttttc cttggactcc agttcgtagg cgcgcctcag
     2761 caggtcatga acgctcaggc cctgcttgtg cactctcagt atctcgatga cggctggttt
     2821 tgaaatctcg tcgtcgtcga ggaaatccag aagcgcgttc gcgttgtaac tgttgctgct
     2881 cgagagtgcc catgcggtgc ccgagaccac ccggtcgccg ccgtccgcga gcccctcttt
     2941 gtaatacttg agcgtcttgt cgctcacctg gctcgccagt atgtcgacaa gcagcatggt
     3001 gtggctgcgg tcgcacatgg cgagcgcgtc gatggctttg gggatcacct tcggcccaag
     3061 cttcttcagc cgctcggtga gtttgagggc gttggacgag gctgtgtccg gttcgccgat
     3121 aagttgggtg atcagctggt cggcgcgaaa acccgctagt atcgccatct gtcccaattc
     3181 ccccgggtgc aggcgtccat tccctccgtg gcgccggcgc tactaacgcg aacagggaca
     3241 gaaaagccgc cggctgacgg catccctgcg cttgcatgca gctgtacctg gtgttgtaaa
     3301 actgtcaatt cgttgtgtcg ctcaagccgg attgattgag gctcctggtg cggcattatg
     3361 ccatatcgct gccgtcgcga ttcggagaca ggagcatcgg ccgaagtggc cggactgccg
     3421 acaccatatc aacgcaacct cggctacaat gtcccgtgcg tcgagccggc gcctgctccg
     3481 acaggtatct tcatcggctc cgtggccgga acttaccctg ttttcgcaat catgaagtgt
     3541 gaaattgcca gcaaattccg atcttaagca agcgattgag acgagcaagg tgcccggtac
     3601 ggagcacctg cttcgcgatg tggggcgcgt caagcgcgcc agcgtggaag atggacgcgc
     3661 ccgcatcgag atcgaactcg ggtttccagc agaaacggcg caggtggaac tgcggaagtt
     3721 tttccaggat ttggcgaaaa ccgccagcgg cgcagaagac gtcgaggtgg tgctctccag
     3781 caaaatcgcg tcccacggcg tgcagccgaa cctgaagccg ctggcgaacg tcagcaacat
     3841 catcgcgatc gcatccggaa agggcggggt cggcaaatca acgacggcgg cgaacatcgc
     3901 cctcgcgctg tcccgtgacg gcgcttcggt cggggtgctg gatgccgaca tttacgggcc
     3961 ttcccagccg ctcatgctcg ggctttccgg gcagaggccg accagcgcgg atggcaaaac
     4021 aatgattccg cttcaggcgc acggcctgca agtcatgtcc atcggcttcc tgatcgacgc
     4081 cgaccagccg atggtatggc gcggcccgat ggtgacccag gctctcaacc agctgctgca
     4141 acagacgcag tggacagacc tggactacct gattgtcgac atgccgcccg ggaccggcga
     4201 tatccagctg accctgtccc agcgcgtgcc ggtgagcggg gccgtaattg ttacgacgcc
     4261 gcaagatatc gcgctgctgg atgcgcgcaa ggggctgcag atgttccgca aggtatcggt
     4321 tccggtgctc ggcatagtcg aaaacatgag cacgcacgtc tgcagcaact gcggcttcga
     4381 ggagccgatc ttcggtagcg gcggcgggga gcgcatggca agggattttg acgtgcagct
     4441 tctcgggcag ttgccgctcg acttgcagat ccgggagcag accgacagcg gtacgccgac
     4501 ggtggtagcg gagcctgaca gcccgccggc gcaggcctat atgcagaccg cgcggcggat
     4561 ggctgcgcgg ttggcgatgc agggcaagga ctacagtcgc aagtttccga atatcgtggt
     4621 cgaggacagc tagcctccta caggtgccaa tcgtcgagcg gcgcgggatc ggcggcttaa
     4681 caagagctaa gggacactca acgtgagcat aaaatcggat cgatggatcc gccgcatggc
     4741 agtggagcac ggcatgatcg atcccttcga gccggcgcag gtgcgcagca actccggggg
     4801 gcgcatcgtc tcttacggta cgtccagcta cggctatgac gtccgctgtt cggacgaatt
     4861 caagatcttc accaacatcg aatccgtcat cgtcgacccc aag
//
LOCUS       DASUOM010000537         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1486660,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000537 DASUOM010000000
VERSION     DASUOM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486660"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2548)
                     /gene="putA"
                     /locus_tag="VFY27_11190"
     CDS             complement(<1..2548)
                     /gene="putA"
                     /locus_tag="VFY27_11190"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX5766123.1"
                     /translation="MRELAQAADAGEPLRKKIQDTAELLVHHVRKNAVADGGLDAFLQ
                     QYDLSSEEGVLLMCIAEALLRIPDADTADELIADKISSASWQDHIGASDSLFVNASTW
                     GLMLTGRLLKLDEATRRNPVQVLKKLAGRAGEPVVRTAMRQAMRIMGHQFVMGRTIDE
                     ALERSKQKKRRHYRYSYDMLGEAALTTRDAARYFDSYSSAIRSIGAAANRVQDLLAAP
                     SISIKLSALHPRYTFNQAERVLKELVPRVADLVIEAREAGIALTVDAEEADRLELSLD
                     VFARVYRDSRLSGYDGLGLAVQAYQRRATDVIRFLRTMAGEAGRRIPVRLVKGAYWDT
                     EIKRSQEHGLDSYPVFTRKSHTDVSYLACARLLLEAGESLYPQFATHNAHTLASVIHF
                     AKGSSRFEFQRLHGMGEDLYRQVVDPEKFNLPCRVYAPVGSHEDLLPYLVRRLLENGA
                     NTSFVNRIVDENVAVSDIIADPVALARKSGCRPHPGIPAPGDLFMPERRNSRGYNLAD
                     RAVSRTLLIATDRAADKVSTAASRKAARGTETRSLNPADNTHVVGTWKAAGTQAVDKA
                     IENALSAQPRWDQRPASERAEILRRAADLFESHTPEILALCILEAGKTVPDSIAEIRE
                     AVDFLRYYAAGCVTLFGDPVVLRGPTGERNTLGMRGRGVFVCISPWNFPLAIFTGQVA
                     AALAAGNAVLAKPAEQTTLIAARAVELLLEAGMPPDVLQLIPGEGAKTGAYAVADPRI
                     AGVAFTGSTETARSINRTLAARDGSIAVLIAETGGQNAMFADSTALPEQVVLDSVMSA
                     FNSAGQRCSALRVLCLQEDIAPRVIELLRGYMRELRIGAPGDLGVDIGPVI"
BASE COUNT          454 a          838 c          768 g          496 t
ORIGIN      
        1 cgatgaccgg gcctatgtca acgccaagat cgcccggggc gccgatcctg agttctcgca
       61 tatagccacg caacagttca atcacccgtg gcgcgatatc ctcctgcagg cacaggacgc
      121 gcaacgcaga gcagcgttgc cctgcactgt tgaatgccga catgacgctg tccagtacaa
      181 cctgctccgg cagcgccgtg ctgtcggcga acatggcgtt ctggcccccg gtctccgcga
      241 tcaggactgc aatcgaaccg tcgcgcgcgg cgagcgtgcg gttgatggaa cgggcggttt
      301 ccgtggagcc ggtgaacgca actccggcaa tcctcggatc cgcaaccgca tacgcgcccg
      361 tcttcgcacc ctctcccggg atcagttgca ggacgtccgg cggcatgcct gcctccagga
      421 gaagctcgac ggcccgcgcg gcgatcaacg tggtttgttc cgcgggtttg gcgagaaccg
      481 cgttgcctgc cgccagtgcg gccgcaacct gtcccgtgaa aattgccagc gggaaattcc
      541 agggactgat gcatacgaaa acgccgcgcc cacgcatccc gagggtgttc ctttcgccgg
      601 tcggccccct cagcacgacg ggatcgccga acagcgtgac gcagcctgcg gcgtagtagc
      661 gcaggaagtc gacggcctcg cgaatttcgg caatagaatc cgggactgtc ttgccggcct
      721 cgaggatgca aagggccagg atttccggcg tatggctttc gaacagatct gcagcgcgac
      781 gtaggatttc ggcgcgctcg gatgccggac gctggtccca tcgcggctgg gccgaaagcg
      841 cattttcaat cgctttgtcg actgcctgcg tccccgcggc tttccacgtg ccgactacgt
      901 gtgtgttgtc cgccgggttt aatgaccgcg tttccgtgcc ccgggctgcc ttcctgctcg
      961 ccgctgtcga cactttgtcg gcagcgcgat ccgtcgctat caaaagcgtt ctgctcaccg
     1021 cgcgatcggc cagattatag ccgcgggaat tgcgacgctc gggcatgaac aggtcgccag
     1081 gcgccggtat gcccggatgc ggccggcacc cggacttgcg cgcgagcgcg accgggtccg
     1141 cgatgatgtc cgaaaccgct acgttctcat caacaatccg gtttacgaac gaggtgttcg
     1201 cgccgttctc gagaaggcgg cgcacgaggt acggcaacag gtcttcatgg ctgccgaccg
     1261 gcgcgtagac gcggcagggc agattgaatt tctccggatc gactacctgg cgataaagat
     1321 cctcgcccat gccgtgcagg cgttgaaact cgaaacggga actccccttc gcgaagtgta
     1381 tgacgctcgc cagcgtgtgc gcgttgtgcg tcgcaaattg cgggtacagg ctttcacccg
     1441 cctccagtaa caaccgcgcg cacgcgaggt aggaaacgtc agtgtgcgac ttacgagtga
     1501 agacaggata gctgtcgagg ccgtgttcct ggcttcgctt gatttcggta tcccagtacg
     1561 ctccttttac caaacgcacc ggtatgcggc ggcctgcctc accggccatc gtgcgcaaaa
     1621 aacgaataac gtcggtggcg cgccgctggt atgcctggac cgccagtccc agcccgtcat
     1681 aaccggagag cctgctgtcc cgatacacgc gcgcgaacac atcgagcgaa agttcgagcc
     1741 gatctgcctc ttccgcatcg actgtcaacg caatgcccgc ttcgcgggcc tcgataacga
     1801 ggtcggcaac ccggggcacg agttccttca gcacccgctc cgcctggttg aaggtgtagc
     1861 ggggatgcag cgccgataac ttgatggaga tgctgggtgc cgcgagcagg tcctgcaccc
     1921 ggttcgctgc tgcgccgatg ctccggatcg ccgacgagta cgaatcaaag tagcgcgcgg
     1981 cgtccctggt cgtcagcgcc gcttcgccca gcatgtcgta cgaatagcgg taatgccgcc
     2041 tcttcttctg cttcgacctc tccagtgcct cgtctatggt gcggcccatt acgaactggt
     2101 gtcccatgat gcgcatcgcc tgccgcatcg ccgtccttac aaccggctcg cccgcgcgac
     2161 cggcaagctt cttgagcacc tgcaccgggt tgcgcctcgt tgcctcgtcc agcttgagga
     2221 gccggccggt cagcatcagt ccccaggtgg atgcgttgac gaacagcgag tcacttgccc
     2281 caatatgatc ctgccagctc gctgatgaga tcttgtccgc gatcagttcg tccgcggtat
     2341 ccgcgtccgg aatccgtagc aaggcttccg cgatgcacat gaggagcaca ccctcctccg
     2401 aggaaaggtc gtactgctgc aaaaaggcat ccaggccgcc gtcggcaact gcgttcttcc
     2461 tgacatggtg cacaagcagt tccgccgtgt cctggatctt ctttcgcaac ggctctccgg
     2521 cgtctgctgc ttgcgccagt tcccgcacca gcgatt
//
LOCUS       DASUOM010000538         5533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1757878,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000538 DASUOM010000000
VERSION     DASUOM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757878"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFY27_11195"
     CDS             <1..1353
                     /locus_tag="VFY27_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5766124.1"
                     /translation="LENPQEAKAIAVKIIDATRAREAARKAREMTRRKGALDIAGLPG
                     KLADCQEKDPALSELFLVEGDSAGGSAKQGRDRRTQAILPLKGKILNVEKARFDKMLS
                     SAEVGTLITALGCGIGRADFDPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPEL
                     IERGHIYIAQPPLYKIKRGKQEVYVKDDAELTAYLLNAALDDARLHVNAEAPALSSAA
                     LEALAKEYTAVESIVSRLANRYGEAALRAIGRLQAVDSRIYENLDRLEAWTEQLSAAL
                     PKLSGGRGDNGNGGSYTAALDRGDSDGEGVRIEIRKVQHGMGTTRYLPREFFDTNEYK
                     HIVSLGTKLNDLIGPGAYIARGDKQLQISSFAEAMEWLMNEARRGQSVQRYKGLGEMN
                     PAQLWDTTVNPETRRLMQVKIEDAVKADEVFTTLMGDQVEPRREFIERNALSVSNLDV
                     "
     gene            complement(1395..2102)
                     /locus_tag="VFY27_11200"
     CDS             complement(1395..2102)
                     /locus_tag="VFY27_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5766125.1"
                     /translation="MLLLRSLIFQVYFFVSVLLFACAVFLSWPLSFERRFALARTWAR
                     SMLWVGRVVCGLNYLIEGEENIPDEPSVIMIKHSTVFEAYAQLAVFPPQTWVIKRELT
                     WIPIFGWGVAAMKPIAIDRRAGHTAVSQVIEQGRARLREGIWVTIFPEGTRVQRGMVR
                     KYGVSGAALASDVGCPVLPVAHNAGDLWPRRSLIKRPGLIRFCIGPAIDSAGRSPREI
                     NESVRLWIENKMDEISA"
     gene            complement(2102..2677)
                     /gene="gmhB"
                     /locus_tag="VFY27_11205"
     CDS             complement(2102..2677)
                     /gene="gmhB"
                     /locus_tag="VFY27_11205"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX5766126.1"
                     /translation="MTGSALRLIVLDRDGVINHDSGEFIKSAAEWKPIEGSLEAIALL
                     SASGFTVAVASNQSGIGRKLFDRSALDSIHRKMRRAVQAAGGSIDSIVYCPHLPDDHC
                     DCRKPAPGLLRRLGRRYGVPMEGVPVIGDSDRDLAAARAAGARPVLVLSGNGRRTKAE
                     LDRLRVPVEHYDNLLAAAKALVSEVEPGKRP"
     gene            complement(2695..4785)
                     /gene="glyS"
                     /locus_tag="VFY27_11210"
     CDS             complement(2695..4785)
                     /gene="glyS"
                     /locus_tag="VFY27_11210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5766127.1"
                     /translation="MTAEGKAADFLVEIGTEELPPKALKELMLAFARNVEEGLARQRL
                     DHEGVTPFASPRRLAALVTRLSRSQPGRELEQKGPPLSVAFDAQGLPTPAARAFAEKC
                     GVPVEALRRSSTPKGEWLSCRVAEAGQSAASLLPGICEEALEKLPVPRRMRWGSSDYE
                     FVRPVHWVLMLHGTEVIDARLLGVRAGRLTRGHRFLSTGELEVAEPASYLGVLEQAFV
                     IAGFPERRQRILRSVQDAAAGAGGVAIGDDDLYDEVTALTEWPVALAGRFSDTFLELP
                     PQVIIATLTGHQRYFPVRSGDGELLSAFVTVANLESRDPGRVRDGNERVVQPRLADAA
                     FFWEQDRKASLASRRGALGGIVYQKGLGTLADKSERVASLSAAISGQLQIGASTVQRA
                     ALLAKCDLLTGMVGEFPELQGTMGRYYALGSGESQSVADAIGEQYLPRFAGDSLPVTQ
                     AGQVLAIADKLDTLAGIFSLGRKPSGNRDPFGLRRAALGLVRIVIENKLDLDLKRLIA
                     RAVELQPAQPRDATEPGGDIYDFILERMRAYYIDQKGLAPEIFEAVRVRRPVSLSDLD
                     KRVDAVAAFVKVDAAQSLAASNKRIANILRQAGDHAYGAVDASLLEEDAEVELFDAIR
                     SAQASVDPLLQTGAYEAVLTRLAQLRQPVDTFFDKVMVMTDDEALRHNRLALLSGLRG
                     LFLDIADISRLSVG"
     gene            complement(4786..>5533)
                     /gene="glyQ"
                     /locus_tag="VFY27_11215"
     CDS             complement(4786..>5533)
                     /gene="glyQ"
                     /locus_tag="VFY27_11215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5766128.1"
                     /translation="QPCRRPTDGRYGDNPFRLQHYYQYQVVIKPSPLEIQELYLESLQ
                     AIGIDLAVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECRPVTG
                     EITYGLERLAMYLQNVESIFDITWTDGPLGRITYRDVYHQNEVEQSRYNFEEADTSAL
                     FRQFDHAEAESRRLIECKLALPAYEQMLTASHTFNLLDARRAISVSERQRFILRVRGM
                     SRAIAESYYASREALGFPMVHTVMQKKRGS"
BASE COUNT         1049 a         1803 c         1725 g          956 t
ORIGIN      
        1 ctcgagaatc cgcaggaggc gaaggcgatc gccgtcaaga tcatcgacgc cacgcgggcc
       61 cgcgaggccg cacggaaggc gcgggagatg acgaggcgta agggtgctct cgatatcgca
      121 ggtctcccgg ggaaactcgc ggattgccag gaaaaagatc cggcgctctc agagttgttc
      181 ctggtcgagg gcgactccgc aggcggctcc gcaaagcagg gcagggatcg ccggacacag
      241 gctatccttc ccctgaaggg aaagatcctg aacgtcgaga aggcgcgctt cgacaagatg
      301 ctgtcatcgg cagaagtcgg caccctgatc acggcgctcg ggtgcggcat cggccgcgca
      361 gatttcgatc cggacaagct gcgctatcac cgcgtgatca tcatgaccga cgccgacgtc
      421 gacgggtcgc acatccgtac cctgctcctg acgttcttct atcggcagat gcccgagctg
      481 atcgaacgag ggcacatcta tattgcgcag ccgccacttt acaagatcaa gcgaggcaag
      541 caggaggtgt acgtgaagga cgatgcggag cttacggcct atctcctgaa cgctgccctg
      601 gatgacgctc gcctgcacgt caatgcagaa gcgcccgcct tgtcgtcggc cgccctggaa
      661 gcactggcga aggaatacac cgccgtcgaa agcatcgtgt cgcgactggc caatcgctat
      721 ggcgaggcgg cgctgcgggc gatcggcagg cttcaggcag tcgattcccg gatatatgaa
      781 aacctggaca ggctggaagc ctggaccgag cagctgtcgg cggcgctgcc gaagctctcc
      841 ggagggcgcg gtgacaacgg taacggcggc agctacaccg ccgcgctcga ccgcggcgac
      901 agcgacggcg agggagtgcg tatcgagata cgcaaagtcc agcacggcat gggcacgacg
      961 cgctatcttc cgcgggaatt ctttgacacc aacgagtaca agcacatcgt ctcgctgggg
     1021 acgaagctca acgacctcat cggcccaggg gcatacattg cgcgaggcga caagcagctg
     1081 cagatcagct ccttcgccga ggctatggag tggttgatga acgaagctcg tcgaggacag
     1141 tccgtgcagc gttacaaggg actcggggaa atgaatcccg cccagctctg ggatacgaca
     1201 gtgaacccgg agacccggcg cctcatgcag gtgaagatcg aagacgccgt caaggccgac
     1261 gaagtcttca ccaccctgat gggcgaccag gtggagccgc ggcgcgaatt catagaacgg
     1321 aacgccttgt ctgtctccaa cctcgacgtc tgacggctgc agggccggct ttgccggccc
     1381 tgcctctcca cccgttacgc gcttatctcg tccattttgt tttctatcca gaggcgcacc
     1441 gactcgttga tctcacgcgg cgagcgaccg gcgctgtcga tcgccggccc gatgcagaag
     1501 cggatgagcc cgggtcgctt gatcaggctg cggcgaggcc aaaggtctcc ggcgttgtgt
     1561 gcaaccggca gtaccgggca tccgacgtca ctggcaagcg ccgcgccgct gacaccgtat
     1621 ttccgcacca tgcccctctg cacgcgcgtt ccctccggaa aaattgtcac ccagatgcct
     1681 tcccggagcc tcgcgcggcc ctgctcgatg acctgggaca cagcggtgtg gccggccctc
     1741 cggtcgatcg cgatcggctt catcgcggcc actccccaac cgaagatcgg gatccatgta
     1801 agctcccgct tgattaccca ggtctgcggc gggaataccg ccagctgcgc ataagcctcg
     1861 aacaccgtcg aatgcttgat catgatgacc gaaggttcgt cagggatgtt ctcctcgcct
     1921 tcgatcaggt agttgagccc gcatacgacg cgaccgaccc acagcatcga acgcgcccag
     1981 gtacgggcaa gtgcgaagcg ccgctcgaag gaaagcggcc agctcaggaa gacggcgcac
     2041 gcgaacagca gaaccgagac gaaaaaataa acctggaaga tgagcgaacg cagcaggagc
     2101 atcacggccg cttcccaggt tcaacttcgc tcaccagcgc ttttgccgcc gcaagcaaat
     2161 tgtcatagtg ctcgaccggc acccgaagcc gatccagctc agccttggtt ctgcggccgt
     2221 tcccgctcaa caccagcacc ggtcttgccc ctgcagcccg tgcggcagcg aggtcgcgat
     2281 cgctgtcccc gatgaccggg accccctcca tcggcacccc gtagcgccgc ccgaggcgcc
     2341 tgagcaatcc cggcgccggt ttgcggcagt cgcaatggtc gtccggcagg tgagggcagt
     2401 aaacgatgct gtcgatagag cccccggcgg cctgcacggc ccgccgcatt ttgcggtgaa
     2461 tcgagtcgag ggcgctgcgg tcgaaaagct tgcgaccgat gcccgactgg ttggacgcca
     2521 cggccaccgt gaaaccgctt gccgacagca gggcgatggc ttcgaggcta ccctcgatcg
     2581 gcttccattc ggcggccgac ttgatgaact cgccggagtc gtggttgatg acgccgtccc
     2641 tgtccagcac tatcaaccgt aaggccgatc cggtcatgaa ggccgcctgc gctgtcagcc
     2701 gacggaaagg cgagagatgt cggcgatgtc gagaaacagg ccgcgaagcc cggacagcaa
     2761 tgccagccgg ttgtgccgca gcgcctcgtc atcggtcatc accatcacct tgtcgaagaa
     2821 ggtgtccacc ggttgccgca gttgtgcgag cctcgtgagg actgcctcgt atgcgccggt
     2881 ttggagcaac gggtcgacag aggcctgcgc ggagcggata gcgtcgaaaa gctcgacctc
     2941 cgcatcctcc tcgagcaggg aagcatctac tgcgccatag gcgtggtcgc cggcctgccg
     3001 cagtatgttg gcgatgcgct tgttggatgc ggcaaggctc tgcgccgcgt cgaccttgac
     3061 gaatgccgcc actgcatcga cacgtttatc aaggtcggac aaagacacgg gacgcctgac
     3121 gcgcacggct tcgaaaattt ccggcgccag gcccttctgg tcgatgtagt acgcccgcat
     3181 ccgctccagt atgaaatcgt agatatcacc gcccggttcc gtggcgtctc gcggctgcgc
     3241 tggctgaagc tcgaccgcgc gcgcaatgag cctcttcaag tcgaggtcga gcttgttctc
     3301 gatgacgatg cgcacgagcc ccagtgctgc ccggcgaagg ccgaaggggt cgcggttgcc
     3361 gctgggcttc ctgcccaacg agaaaattcc cgcaagcgtg tcgagcttgt ccgcaatggc
     3421 aagtacctgc ccggcctgcg tcacgggcag cgaatcgccg gcaaaccggg gcaggtattg
     3481 ctcgcctata gcgtccgcga ccgattggga ctcgccggag ccgagggcgt aatagcgtcc
     3541 catggtgccc tggagctcag ggaattcccc gaccatcccg gtgagcaggt cgcacttcgc
     3601 caggagcgcg gcgcgctgca ccgtcgaagc cccgatctgc aactgcccgg agattgcagc
     3661 cgagaggctc gccacccgct cgctcttgtc agccagcgtg cctaagccct tctggtacac
     3721 gatgccgccc agtgctcccc gcctgctcgc gagcgacgct ttcctgtcct gttcccagaa
     3781 aaacgcagca tctgccaggc gcggctgcac cactcgttcg ttcccgtcgc gcaccctgcc
     3841 cgggtccctg ctctccaggt tggcgacagt aacgaaggcg gagagcaatt cgccgtcccc
     3901 gctcctgacg ggaaagtagc gctggtgacc ggtcagggtg gcgatgatca cctgcggcgg
     3961 cagttcgaga aatgtgtcgc tgaaacggcc agccagcgca accggccact ccgtcaaagc
     4021 cgtgacttcg tcgtaaaggt cgtcgtctcc gattgccaca cccccggcgc ccgccgcggc
     4081 atcctggacg gatcgcagga tccgttggcg gcgctcggga aagcctgcga tgacgaatgc
     4141 ctgttcgagc acgcccaggt aggaagctgg ctccgctacc tcgagctcgc cggtcgagag
     4201 aaagcggtgt ccgcgagtca gcctgccggc ccgtacgccg agcaggcggg cgtcgatcac
     4261 ctccgtcccg tgcagcatca ggacccaatg caccggcctt acgaactcgt agtcggaact
     4321 gccccagcgc atgcgccgtg gcacgggcaa cttctcgaga gcctcctcgc aaatgcccgg
     4381 cagcagcgag gctgctgact gtccggcttc ggcgacgcgg caggaaagcc actcgccttt
     4441 gggggtgctg ctgcgtcgga gagcttcgac cggaacgccg catttttccg caaaagcgcg
     4501 ggctgcgggg gtgggtaagc cctgcgcgtc gaacgcgacc gacagcggag ggcccttttg
     4561 ctccagttcg cgccccggct gcgacctcga gagcctggtc acgagcgcgg cgagccgccg
     4621 cgggctggcg aacggcgtga cgccctcatg gtccaggcgc tggcgcgcaa gcccttcctc
     4681 cacgttccgg gcgaaggcga gcatcagttc cttcaaagcc tttggcggca actcttccgt
     4741 gccgatttcg acgaggaaat cggccgcctt tccttcggcc gtcacttaag agccccgctt
     4801 tttctgcatg acggtgtgca ccattggaaa gccgagcgct tcccggcttg cgtagtagga
     4861 ctctgcaatg gcgcgcgaca tgccgcgcac gcgcaggata aaacgctgcc gctcactgac
     4921 cgaaattgcc cgccgggcat ccagcaggtt gaaggtgtgc gacgccgtca acatctgctc
     4981 gtacgcgggc agcgccagct tgcattcgat cagccgccgg ctctcggcct ccgcatgatc
     5041 gaactgcctg aaaagcgcgc tcgtatcggc ttcctcgaag ttgtagcggg actgttccac
     5101 ctcgttctgg tggtagacgt cacggtaagt gatgcgcccc agcgggccgt ctgtccacgt
     5161 gatgtcgaaa atactctcga cgttttgcag gtacattgcc aggcgctcca gcccatatgt
     5221 gatttcaccg gtgaccgggc gacattcgag accacccacc tgctggaagt aggtgaactg
     5281 ggtcacttcc atcccgttca gccagacctc ccatcccagg ccccacgcgc cgagcgtcgg
     5341 agattcccag ttgtcttcga cgaagcgaat gtcatggacc gccaggtcta tgccgatcgc
     5401 ctgcagcgac tcgaggtaca gctcctggat ttcgagaggc gagggtttga tcacgacctg
     5461 gtactggtag taatgctgca ggcgaaacgg gttgtcgcca taccggccat cggtcggccg
     5521 ccggcaaggc tgc
//
LOCUS       DASUOM010000539         7431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_848761,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000539 DASUOM010000000
VERSION     DASUOM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848761"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFY27_11220"
     CDS             complement(<1..242)
                     /locus_tag="VFY27_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5766129.1"
                     /translation="MSLKGSKTETNLKDAFAGESQANRRYLYFAAKADVEGYNDVAAV
                     FRSTAEGETGHAHGHLEYLEEVGDPATGLPIGATSA"
     gene            399..638
                     /locus_tag="VFY27_11225"
     CDS             399..638
                     /locus_tag="VFY27_11225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5766130.1"
                     /translation="MSGETPLNLEKSLEQLETLVDELESGDLPLETAMKKFEEGIKLT
                     RQCQSALKDAEQRVEVLLKSAGGEEVLEEFSAADT"
     gene            complement(700..2001)
                     /locus_tag="VFY27_11230"
     CDS             complement(700..2001)
                     /locus_tag="VFY27_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5766131.1"
                     /translation="MVRSVTELKRPADVPKKIGKYVIINKVGKGSTGTVYLSHDPYYR
                     RDVAIKVYNIEEDTDQDRARVSRKMFFNEAHMVGMLQHPNIMPIYDAGEEAGKYYVVT
                     EHIQGARTLAAYCRPDNLLRVDDVVEIIYKCAKALHYAHGRGVIHRDIKPSNIMLTTD
                     NDVRIIDFGIAICTDSDVSRIEGIAGSPSYMSPEQVQSEELTSRSDIYSLGAVLYELL
                     TGFRPFRADNLSKLLHQIVYATAPPIHTYRDDLPEELENVVAMCMQKDPGKRCASGAA
                     MAADLTRVHKDLRAKYHSLDNREHFDLLRTLTFFHEFSHAEIWEVLRASDWHEYKGGE
                     DIVREGEMDDRFYIIVAGQADVEANGRKLGVLSNGDCFGETSYVRGAKRTASIRASGN
                     VTILRVSSTLMEQVSSACQLRFNKVFLRSLITRLQGDSANT"
     gene            complement(2017..2517)
                     /locus_tag="VFY27_11235"
     CDS             complement(2017..2517)
                     /locus_tag="VFY27_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5766132.1"
                     /translation="MKKTEAKADAELRRKVLRDPVLLLAFGFGTGLAPRAPGTVGSLL
                     GIPLAWAAIALELHLQLVLGAVLFAIGIWLCGEAGKRLAVHDHPGIVWDEIVAMYLVL
                     LALPVSIVFWTAGYLLFRLFDIWKPWPIRDLDHRLGGGLGTMLDDLFAALYAALLLGS
                     GRWLMF"
     gene            complement(2521..3480)
                     /gene="thiL"
                     /locus_tag="VFY27_11240"
     CDS             complement(2521..3480)
                     /gene="thiL"
                     /locus_tag="VFY27_11240"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5766133.1"
                     /translation="MDEFDLIRRFFVREGAGDGVIVGVGDDGAIIEPEPGRNLVVVVD
                     TLIEGVHYPFDLSPADVGYRSLAVNLSDVAAMGGRPRWMTLALTLVEADETWLCGFAN
                     GLFEAAGEHAVALIGGDTTRGAQTVISVQLIADVVSGDALARSGARPGNAIYVTGTPG
                     DAAGGLSLLQEHSKERDDSDGSYLVHRFSRPSARVAFGAAIGGIATAAIDLSDGLGTD
                     LAKLLAASNVSGVLDLGLLPLSAEILRVFDRERAMSFALDGGDDYELCFTAPPADEHQ
                     IMAMAARQGVTVTLIGEVGEGSGLACKRDGKLFPYEDRGYRHF"
     gene            complement(3513..3959)
                     /gene="nusB"
                     /locus_tag="VFY27_11245"
     CDS             complement(3513..3959)
                     /gene="nusB"
                     /locus_tag="VFY27_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018137845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5766134.1"
                     /translation="MSDTPSPGPRGRARELLVQALYQLQIADHEKRELLAQFRERPEY
                     QRVDQSFFDEALDEILEGREALEKKAEEYLDRPLSQLDPVERGILLLGFYELRSRMEI
                     PYRVVINEAVNLTKRFGAVDGHKYVNAVLDRAAGHMRQGERGGASR"
     gene            complement(4024..4497)
                     /gene="ribE"
                     /locus_tag="VFY27_11250"
     CDS             complement(4024..4497)
                     /gene="ribE"
                     /locus_tag="VFY27_11250"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5766135.1"
                     /translation="MDKIKTIEGELLVRDARITIVASRFNSFVVDALVRGAVSCLRRH
                     GAVDADLELVRVPGAYEMPLAVDKIAAARRNDGIVALGAVIRGATPHFDYVAGECVRG
                     ISEAGRKHGVPIGLGVLTVDTIEQAIERSGTKAGNKGEEAALAVIEMVNLLRRLS"
     gene            complement(4693..5832)
                     /gene="ribBA"
                     /locus_tag="VFY27_11255"
     CDS             complement(4693..5832)
                     /gene="ribBA"
                     /locus_tag="VFY27_11255"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX5766136.1"
                     /translation="MHNKTSIHPSAATFSSTEDIIADIRAGKMVIMVDDEKRENEGDL
                     IMAAEKVRAQDINYMARYGRGLICLTMTRERCAQLRLPLMVDDTDRRHATNFTISIEA
                     AEGITTGISAHDRARTVQVAVAPDARPEHLSQPGHIFPVMAQPGGVLTRAGHTEAGCD
                     LARLAGFEPAAAIVEILNEDGSMARRPDLEAFSRQHGVRIGTIADLIRYRLEKERYVE
                     RIAEKTVQTAHGEFRMYCYDDRVNRTVHVALAKGDLARATLPLVRVHIQDTLGDILGV
                     QSPTLGWPLDSAIERISREDTGVVVVLREQESSRELMEAVESLSQTSDDLAERRRGEA
                     VIKTYGVGAQILRDLGLQRIRVLSAPKQMHGISGFDLEIVEYVEK"
     gene            complement(5825..6493)
                     /locus_tag="VFY27_11260"
     CDS             complement(5825..6493)
                     /locus_tag="VFY27_11260"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5766137.1"
                     /translation="MFTGIVKALGVVEQMQVRDEGARLSVRSGDLPWRDYGVGDSISV
                     NGVCLTAAELHEDGFDADLSLETMTVTAMRNLASGSSVNLEPSLALGERLGGHLVSGH
                     VDCVGTVLRRTEDAGHVRLRTGIPEQYLRYVVKKGSICVDGVSLTIHEVSSNPAAWFE
                     VTIIPHTSAATIAGTYDIGAEVNIEVDLIARYLEGLLGTGYATPAETSQGITKDFLRA
                     YGYA"
     gene            complement(6495..>7431)
                     /gene="ribD"
                     /locus_tag="VFY27_11265"
     CDS             complement(6495..>7431)
                     /gene="ribD"
                     /locus_tag="VFY27_11265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5766138.1"
                     /translation="ALASAGDRARGATAYVTLEPCRRTGRTGPCSEALVRAGVAKVIF
                     ASGDPSQRDAGQASALEQAGIEVRGGLMQAEAEKLNEGFLMRVSQQRPFVRLKVASSL
                     DGATAMQSGESRWITGEAARRDVQKLRATSGAIMTGVGTIVADDPLLTVRDESLVLRQ
                     PFRVVIDSRLSTPAAARVFEQPGETLIFCTEDRGRGTLEQRGAAVYRVATHRGRVDLA
                     EVLRKLAELEVNDVLVEAGPLLSGSLLAGGHVDELVIYQAPHMMGSETMGIFTTPGWT
                     ELGHRLPLEFLDVRRVGRDARIIARPAAKEPAAAN"
BASE COUNT         1370 a         2449 c         2172 g         1440 t
ORIGIN      
        1 ttcgccgagg tagcgcctat cggcaggccc gtagccgggt cgcccacctc ctcgagatac
       61 tcgagatggc cgtgcgcgtg tccggtttcg ccctctgccg ttgaacggaa taccgcggct
      121 acgtcgttgt agccctcgac atccgctttc gcagcaaagt aaaggtagcg acggtttgcc
      181 tgggactcgc cggcaaaagc gtccttcagg ttggtctcgg ttttgctgcc tttcaatgac
      241 atgatcactc ctcacaaaag cgtcgggttg taactttaga ctaagtctaa tgacccagtg
      301 actctaagcc tgcaggtcac tgatgtcaac gcgttgaccc ccgatcctgg gccatgtagc
      361 gtacccgcga atttcctgaa ggacgcacac ggaatccgat gtccggcgag acacccctga
      421 acctcgaaaa gtccctcgag cagcttgaaa cgctcgtcga cgagctggaa agcggcgacc
      481 tgccgctgga aaccgcgatg aagaagttcg aggaaggcat caagctgacg cggcaatgcc
      541 agtcggcgct caaggacgcg gaacaacggg tagaggtgct gttgaagagc gcgggtggcg
      601 aggaagtgct cgaggaattc agcgccgcgg acacctgagc agatttatct cccccctttg
      661 cgccggcatg gccggctcct acggtgcgct attggcgttt caggtgttgg cgctgtcgcc
      721 ctgcaggcgc gtgatcaggg accgcaggaa gaccttgttg aagcgcagct ggcaggctga
      781 cgatacctgt tccatgagcg tcgagctcac gcgcaggatc gtgacgttgc cgctggcacg
      841 aatagatgcg gttcgcttgg cgccgcggac gtagctcgtt tcgccgaagc agtcgccatt
      901 cgacaatacc ccgagcttcc tgccgttcgc ctccacatcc gcctgtccgg ccacgatgat
      961 gtaaaagcgg tcgtccatct cgccttcgcg gacgatgtcc tcaccgccct tgtactcatg
     1021 ccagtcgctg gcgcgaagca cttcccatat ttccgcgtgg gaaaactcgt gaaagaacgt
     1081 cagggtgcgc agaagatcga agtgttcccg gttatccagg ctgtggtatt tcgcgcgcag
     1141 gtccttgtgc accctggtca ggtcggcagc cattgccgcg ccgcttgcac agcgtttgcc
     1201 gggatccttt tgcatgcaca tcgcgacgac gttctccagc tcctccggca agtcgtcgcg
     1261 gtacgtatgg atcgggggcg ccgtcgcgta aacgatctgg tgcaaaagct tcgacaggtt
     1321 gtcagcgcgg aacgggcgaa acccggtcag cagttcgtaa agcaccgcac ccagggagta
     1381 gatgtcggag cgcgaagtca gttcctcgga ctgcacctgt tcgggcgaca tgtagctcgg
     1441 cgatcccgcg ataccttcta tccgcgagac gtcggaatcg gtacagatgg caatgccgaa
     1501 gtcgatgatg cgcacgtcat tgtcggtcgt cagcatgatg ttgctgggct tgatgtcgcg
     1561 atggatcacg ccgcgcccgt gggcatagtg cagcgccttt gcgcacttgt agatgatctc
     1621 gacgacgtcg tcgacccgca ggagattgtc cggccggcaa taggcggcca gcgtgcgggc
     1681 gccctggatg tgttccgtaa cgacgtagta cttgcccgct tcctcgcccg cgtcgtagat
     1741 cggcatgatg ttcggatgct gcagcatgcc gaccatatgc gcttcgttga aaaacatctt
     1801 gcgggaaacc cgggccctgt cctggtccgt gtcctcttcg atgttgtata cctttatcgc
     1861 gacgtcgcgc cggtagtagg gatcgtggga gagatagacg gtaccggtgc ttcccttgcc
     1921 gaccttgttg atgattacgt acttgccgat tttcttcggg acatcggcgg gccgcttcag
     1981 ttcggttaca ctacggacca ttttgtctta gcctgatcag aacatgagcc acctgccaga
     2041 cccgaggagt aacgcggcat agagggcggc gaagaggtca tcgagcatag ttccgaggcc
     2101 gccgccgagt ctgtgatcca ggtcccgtat cggccagggt ttccagatgt cgaacagcct
     2161 gaacagcagg tagcccgctg tccagaagac aatagaaaca ggaagtgcga gtaaaaccag
     2221 gtacatggcg acgatttcgt cccagacgat tcccgggtga tcgtgcacgg cgaggcgctt
     2281 gcccgcctct ccgcacaacc atatcccgat agcgaagagc accgccccga gtacgagctg
     2341 caggtggagc tcaagcgcaa tcgcggccca cgcgagcgga atcccgagca gcgacccgac
     2401 ggtgccgggc gctcttggcg ccaaccccgt gccgaagccg aacgcgagca gcagcacagg
     2461 gtcgcgaagc accttccggc gcagttctgc gtccgctttg gcttccgtct ttttcatggc
     2521 ttagaaatgc cgatagccgc gatcctcgta cggaaacagc ttgccgtctc ttttgcaggc
     2581 aaggccgctg ccctctccga cttccccgat gagcgtgacc gttacgccct gccgcgccgc
     2641 catagccatg atctggtgct catccgccgg cggcgcggta aagcagagct cgtagtcgtc
     2701 gcctccatcg agcgcaaaac tcatcgctcg ctcgcgatcg aagacgcgca gtatttctgc
     2761 ggatagcggc agcaggccga gatcgagaac gcctgacacg ttgctcgcgg ccagcagctt
     2821 cgcaagatcg gttcccaggc catcggacaa gtcgattgcg gcagttgcaa tcccgccgat
     2881 ggccgcgccg aacgcaaccc gtgcagacgg tcgcgaaaac cggtgaacaa ggtagctgcc
     2941 gtcgctatcg tcgcgctctt tcgagtgctc ctgcagcaat gagagtcccc ccgctgcgtc
     3001 gccaggagtg ccggtaacgt agatcgcgtt accgggccgg gcaccgctac gcgcgagcgc
     3061 atccccggaa actacgtcgg caatcagttg cacgctgatt accgtctgtg ccccccgcgt
     3121 cgtatctccg ccgatcaatg cgacggcatg ctcgcccgcc gcctcgaaca atccattagc
     3181 gaatccgcag agccacgtct catcggcttc gacaagcgtc agcgcgagcg tcatccatcg
     3241 cggcctgccc cccatcgccg cgacgtcgga aaggtttacg gcgagtgacc ggtagccgac
     3301 atccgccgga gacaaatcga agggatagtg cacaccttca atgagggtat cgacgacgac
     3361 cacgaggttg cggcccggct ccggctcgat tatcgcgccg tcgtcgccca cgccgacaat
     3421 cacaccatcg ccggctccct ctcgcacgaa gaaacgacgg atgagatcga actcgtccac
     3481 accgctttcc cgatcagcgt ccggcggcga agtcagcggc tcgcgccgcc acgctcgccc
     3541 tgccgcatat gtcccgccgc gcggtccaga acggcgttga catacttgtg gccatcgacg
     3601 gcgccgaatc gcttcgtcag gttcaccgct tcgttgatga cgacgcgata gggaatctcc
     3661 atgcgcgacc gaagctcgta gaatccgagc agcaatatgc ctcgctcgac gggatcgagc
     3721 tgtgacagcg gcctgtcgag atactcctct gccttcttct cgagcgcctc gcgaccctcc
     3781 agtatttcgt cgagcgcttc atcgaagaat gactggtcga cccgctggta ctcgggccgc
     3841 tcccgaaact gcgccagcaa ctcgcgcttt tcgtgatcgg cgatctgcaa ttggtacagc
     3901 gcctgcacga gcaactcacg tgcacggcct cgcggtccgg gagagggggt atcgctcata
     3961 gctccgacgg ccaccggcgc agcgcattga cgaacaacgc aggtagcatg gacgtaattc
     4021 gcttcaagac aggcgacgca gcaggttcac catctcgatg accgcgagcg cggcttcctc
     4081 gcccttgttt cccgctttgg tgccagagcg ctcgatcgcc tgctcaatag tatcgaccgt
     4141 cagcactccg agcccgatcg gcacgccgtg cttgcgccct gcctccgaaa ttccccgaac
     4201 gcactctccc gccacgtagt cgaagtgcgg cgtcgcgccg cgaatgacag cgcccaacgc
     4261 tactatgccg tcgttccggc gtgccgcggc gatcttgtcg accgcgagag gcatctcata
     4321 ggcacccggg actcgtacca gttcgaggtc tgcatccact gcaccgtgcc ggcgcaggca
     4381 cgagactgct ccccggacga gcgcatccac cacgaagctg ttgaaccgcg atgccacgat
     4441 cgtgatacgc gcatcgcgca ccagcagctc gccttcgatt gtttttattt tatccatctt
     4501 gatgaaacca ggtcctcggt gtcggtgaaa tcgacgtaca cagccagcga acctttgcga
     4561 aagcgcgaag aagcgttgta ggaacccgcc atgcgggcga gtgcgattcg cccgcagcgc
     4621 actcgcccgc acggcgggct cctacagtcg catcaatcat gacctaagca ggattcttcc
     4681 agcggcacta cctcattttt cgacgtattc cacgatctcg agatcgaagc cggaaatgcc
     4741 gtgcatctgc ttcggcgccg acaatactcg tatgcgctgc aagcccaggt cgcgcagtat
     4801 ctgcgcgccg acgccgtagg tcttgatgac cgcttcaccc cgtcgccgct cagcaaggtc
     4861 gtcggaggtc tgtgacaacg attccaccgc ctccatcagc tcccgcgacg actcctgctc
     4921 gcgcagcacg acgaccacgc cggtatcctc tcgcgagatg cgttcgatgg cgctgtcgag
     4981 cggccagccg agcgtcggac tctggacccc gaggatgtcg ccgagggtat cctggatatg
     5041 cacccgtacg agaggcaaag tcgccctggc gagatcgccc ttggcgagcg ctacgtgcac
     5101 cgtgcggttt acgcgatcgt cgtagcagta cattcgaaac tcgccgtgcg cggtctgcac
     5161 cgtcttttcc gcgatcctct cgacgtagcg ttccttctcc agccgatagc gaatgagatc
     5221 cgcgatcgtg ccgatccgca ccccgtgctg ccgcgagaac gcttcgaggt ccggccggcg
     5281 cgccatgctg ccgtcttcgt tgagaatctc gacaatggca gcagccggtt caaagccggc
     5341 gagccgcgcc aggtcgcaac ccgcctccgt gtgcccggcg cgcgtcagta cgccgcctgg
     5401 ttgtgccatg acgggaaaga tgtgaccggg ctggctgaga tgttccgggc gcgcatcggg
     5461 cgctacggca acctgcacgg ttcgagcgcg gtcgtgcgcc gaaataccgg tcgtgatgcc
     5521 ctcggctgcc tcgatggaga tcgtgaagtt ggtcgcgtgc cggcgatcgg tatcgtccac
     5581 catcagcgga aggcgcagct gcgcgcagcg ctcccgcgtc atcgtcaggc aaatgagccc
     5641 gcggccatat cgcgccatgt agttgatgtc ctgcgcacga accttctcgg cggccattat
     5701 caggtcgccc tcgttctccc gcttttcgtc gtccaccatg atgaccattt tgccggcacg
     5761 gatatccgcg atgatgtcct cggtgctgct gaatgtggcc gcggacggat gtatcgaagt
     5821 cttgttatgc ataaccatat gccctgagaa aatcctttgt aatgccctgc gatgtttcgg
     5881 ccggcgtggc gtagccggtg ccgagcaagc cttctaggta acgcgcgatc aagtccactt
     5941 cgatgttcac ctcggcacca atgtcatagg ttcccgcgat cgtcgccgcg ctcgtatggg
     6001 gaatgattgt tacttcaaac cacgctgccg ggttgctcga tacctcgtgg atggtaaggc
     6061 tgacgccgtc gacgcaaatc gatcccttct tcacgacata gcggagatat tgctccggga
     6121 tgccggtgcg caagcgcacg tgccccgcat cttccgtcct gcggagcacg gtgccgacgc
     6181 agtcgacgtg gccactgacc aggtgccccc caagccgttc gccaagtgcc agcgatggct
     6241 cgagattgac gctgctgccg gacgccaggt ttcgcatggc ggtgacggtc atcgtttcca
     6301 atgacaggtc ggcatcgaag ccgtcttcgt gcagctctgc ggcagtcaga cagaccccgt
     6361 tgacggaaat gctgtcgccc acaccgtagt cgcgccacgg cagatccccg gagcggaccg
     6421 atagcctggc gccctcatcc cgcacctgca tctgctcaac cactcccagc gccttgacga
     6481 tgccagtgaa catttcagtt cgccgccgca ggttctttcg ccgcgggccg cgcgattatc
     6541 cgcgcatccc gccccacgcg ccgcacatct aagaactcga gtggcaggcg atggcccagc
     6601 tcggtccagc ccggtgtagt gaagatgccc atggtttcgc tgcccatcat atgcggcgcc
     6661 tggtaaatga caagttcgtc cacatggccc ccggccagca ggctgcccga cagcagcggt
     6721 cctgcctcca ccagcacgtc attgacttcc agctccgcaa gcttccgcaa cacttccgca
     6781 aggtcgacgc gccccctgtg cgtcgcgacg cggtataccg cagcgccccg ttgttccagc
     6841 gtgcctctgc cgcggtcttc cgtgcaaaag atcaacgtct cgccgggctg ctcgaacact
     6901 cgcgccgccg ccggggtgct caggcgcgag tcgatcacca cccggaacgg ttgacgcagc
     6961 accagcgact catcgcgaac cgtgagtagc gggtcgtctg cgacaatcgt ccctacgccc
     7021 gtcatgatag cgccgctcgt cgcacgcagc ttctgcacat cccgccgtgc tgcctcgccg
     7081 gtgatccacc ggctttcacc gctctgcatc gcggtggcgc cgtccaggct ggacgcaacc
     7141 ttcagcctga cgaacgggcg ttgctgcgaa acgcgcatca ggaaaccttc gttcagcttc
     7201 tccgcctctg cctgcatgag accgccccga acctcgatcc cggcctgttc gagcgccgat
     7261 gcctgacctg cgtcgcgttg cgatggatct ccgctcgcga atatcacctt cgcgaccccg
     7321 gcacgaacca gtgcttccga gcacggaccg gttcttccgg ttctcctgca cggctccagc
     7381 gtgacatacg ctgtcgcacc acgcgcgcga tcgccggcgg acgccagcgc a
//
LOCUS       DASUOM010000540         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_186456,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000540 DASUOM010000000
VERSION     DASUOM010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186456"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFY27_11270"
     CDS             <1..524
                     /locus_tag="VFY27_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX5766139.1"
                     /translation="AVSSLRSGKEPDLLEPLHTGVEINMHLPALLPEDYVPDVHLRLM
                     LYKRIASAGSHDELREMQVEMIDRFGLLPAAAKNFMRIAAVKLEASALCIEKIDASRS
                     GGYLVFGKKTLVDPVALVRLVQNDSHTYRMQGAERLQFRLELPDDDDRFTFVEQLLQE
                     LSTDPASARRLAS"
     gene            573..1448
                     /locus_tag="VFY27_11275"
     CDS             573..1448
                     /locus_tag="VFY27_11275"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HEX5766140.1"
                     /translation="MQGITEQQGALARAKANGVHAIALSAQMVCLALFAAAAGAQDEA
                     PLPAGEPRFNVELIVFSYRATDSAGNEVFVPDEPVLVPDAEEELPPDVAQNTFGDVQE
                     PVPEYSDTDITGDAEDERDPVSRRIELQLLDSENHTMSDIYRKLEQLDAYQPIMHAAW
                     TQTTTPREAAPAIHLRALGEPPPGLNGSITLYRGRYLHLVVDLALDASERDSAVTATD
                     RLISYSDGRAQEANGSDRSEPFSQQVRYRILEDRIMKTGDVRYFDHPRFGVVARVTKP
                     KEEQEPADATSGGVD"
     gene            complement(1500..2399)
                     /locus_tag="VFY27_11280"
     CDS             complement(1500..2399)
                     /locus_tag="VFY27_11280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5766141.1"
                     /translation="MKPLQRELLEKLIEASAEPVLTASIERPDWPVVLCNPAFERLHG
                     GKPVLERPLADVVETLLGRDLALEVSETIRSRQETSIPVLLHKREYLLVLRPVSVDSS
                     NNYYALYWRSGHVGDPGAANEDIHHALLRAKRRIRDLSRDDPVTGLLNESAFREVLEH
                     DWAVAAREKSSLALISFTLDNFDAYLEVFGRHATDSCLRRVAQAIRRCMRRASDVAAR
                     VGTGDGDALVVLSHASDEVGVVEFAERISGAVRELGLHHPRSKAARFVTVSHQARVVQ
                     VDRSKQTPAEFLERVITAEEVNA"
     gene            complement(2409..3209)
                     /locus_tag="VFY27_11285"
     CDS             complement(2409..3209)
                     /locus_tag="VFY27_11285"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX5766142.1"
                     /translation="MPEPAQGPAAASFAILAGSGFSDFASLSPGRRLVTAYGAPSAAV
                     REIKFSGRRIWFLPRHGDRHDIPPHRINYRANLAALKLLDVKNVLALNTVGVIAPGPG
                     PGQLAVPDQLIDYTYGRDHSIYDGSSASLDHIDFTEPFSEPLRRQILDAARAADVPCH
                     DGGVYAVTQGPRLETAAEIDRLERDGAGFIGMTAMPEAAIARELQMNYACLSLVVNFA
                     AGRGAKGIHEDIEASLLEAKTRALRLMQQLFNGPSTGTGGGRRDRILE"
     gene            complement(3202..>3620)
                     /locus_tag="VFY27_11290"
     CDS             complement(3202..>3620)
                     /locus_tag="VFY27_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5766143.1"
                     /translation="AGVMLAHVVYVETDPLPAGFSRYWIGNQLRTQLNFDGAVFCDDL
                     SMKAAAEFGPMPKRAALALEAGCDMVLICNDRPAARATVATLRDYSNPLSLVRLARLH
                     GTGQLLRETLLASDEWQAAAQRLGRWSERPELELNA"
BASE COUNT          670 a         1161 c         1155 g          634 t
ORIGIN      
        1 gggcagtcag ttcgctcagg tcaggcaagg agccggactt gctagaaccc ctgcacacag
       61 gcgtcgagat caacatgcac ctgccggcgc tgctgccgga agactacgtg cctgacgtgc
      121 acttgcgact gatgctttac aagcgcatcg caagcgccgg gagccatgac gagctgcgcg
      181 agatgcaggt tgaaatgatc gaccgtttcg gcttgctgcc ggctgccgca aagaacttca
      241 tgcgcatcgc cgccgtcaag ctggaagcat cggccctttg catagagaaa atcgacgcgt
      301 cgcgcagcgg cggctacctg gtgttcggca agaaaacgct ggtcgatccc gtcgctcttg
      361 tccggctggt gcaaaacgac agccacacct accggatgca aggcgcggag cgcctgcagt
      421 ttcgcttgga attgccggac gatgacgata gattcacctt cgtcgagcag ctgttgcagg
      481 agctttcgac tgacccggca agcgcccgga ggctggcaag ttgaacagtc tgcaatccta
      541 aagatctcgt tcgggaacgg aatgacacga gcatgcaagg aataacggaa cagcaaggcg
      601 cattggcgag agccaaagcc aacggcgttc acgcaatcgc cttgtccgcc cagatggttt
      661 gcctggcatt gttcgccgcg gcagcgggtg cgcaggacga agctccttta cctgccggcg
      721 aaccgaggtt caatgtcgag ctgatcgtct tttcgtatcg cgcaacagac tccgccggca
      781 acgaggtgtt cgtccccgac gaaccggtgc tcgtaccgga tgcggaagaa gaattgccgc
      841 ccgacgtcgc gcagaacact ttcggcgacg tccaggagcc ggtgcccgag tattccgata
      901 ccgacataac cggcgacgcg gaggatgaac gtgacccggt gagcaggcgc atcgaactgc
      961 aactgctcga ttccgaaaac cacacgatga gcgacatcta ccggaagctc gagcagctgg
     1021 atgcatacca gccgatcatg cacgcagcat ggacgcagac gacgacgccc agggaagcgg
     1081 ctcccgcgat acacctgcgc gcactggggg aacctccgcc cggcctgaac ggcagcatta
     1141 ccctgtaccg ggggcgctac ctgcacctgg tggtggacct tgctctggat gccagcgaac
     1201 gcgacagcgc agtgacggcg accgacaggc tgatcagcta cagcgatggc cgggcgcagg
     1261 aagccaacgg cagcgatcgg tcagagccat tctcgcagca ggtgcgttat cgcattctcg
     1321 aggaccgcat catgaagacc ggtgacgtgc gctatttcga ccatccccgt ttcggggtcg
     1381 tcgcccgggt cacgaaaccg aaggaagagc aagagccggc cgatgcgacc agcggcggcg
     1441 tcgactgacc cctggccgtc atctcaccgg tctcctgcgc agcgttaaga tcctgaaggc
     1501 taggcgttca cttcttcggc cgtgatgacg cgttcgagga attccgccgg cgtttgcttg
     1561 ctccggtcga cctgcacgac acgcgcctgg tgcgacacgg ttacgaaacg cgccgccttc
     1621 gagcgcggat gatgcaggcc cagttcccgc accgcgccgc ttatccgttc ggcgaactcc
     1681 actaccccga cctcgtctga ggcatgcgac agtacgacga gagcatcgcc gtcgcccgtg
     1741 ccgacgcgtg cagcgacgtc gctcgcgcgc cgcatgcaac gccggatagc ctgcgcgacg
     1801 cggcgcaggc aggagtctgt cgcatggcgg ccgaacactt ccaggtaggc gtcgaagttg
     1861 tcgagcgtaa acgaaatcag cgccaggctg ctcttctccc ttgccgcgac cgcccagtcg
     1921 tgttccagga cttcacgaaa cgcggactca ttcagcaatc ccgtgaccgg gtcgtcgcgc
     1981 gacaggtcgc gaatccgccg tttcgccctc agcagcgcgt ggtggatgtc ctcgttcgct
     2041 gctccggggt cgccaacgtg gccgctgcgc cagtagagcg cgtaatagtt gttggatgaa
     2101 tccaccgaca ccgggcgcag caccagcagg tattcgcgct tgtgaaggag gacgggaatg
     2161 ctcgtctcct ggcgggagcg tatcgtttcg ctgacttcca gcgcgaggtc cctgccgaga
     2221 agcgtctcga cgacgtccgc gaggggacgt tcgagcaccg gctttccgcc gtgcagccgc
     2281 tcgaacgcgg ggttgcagag tacgacgggc cagtccgggc gttcgatgct cgcggtgagc
     2341 accggctccg ccgacgcctc gatcagcttc tccaggagtt cgcgctgcag gggtttcatt
     2401 agctcggact attcaagaat acggtcgcgc ctgccaccac cggtgccggt cgacggtccg
     2461 ttgaacaact gctgcatgag acgcagcgcc cgcgtcttcg cctccagcag gctggcttcg
     2521 atgtcctcgt gtattccctt cgccccgcgc ccagcggcga aattcacgac caatgaaagg
     2581 cacgcatagt tcatctgcag ttccctcgcg atggccgcct caggcatggc tgtcatgccg
     2641 atgaagccgg ctccgtcccg ctccagccgg tcgatttccg cggcggtttc cagccggggg
     2701 ccctgggtca cggcatagac gccgccatcg tgacagggca cgtccgcggc cctggcggcg
     2761 tccaggatct gccgccgcag gggctcgctg aacggttcgg tgaaatcgat gtggtcgaga
     2821 ctagcggaac tgccgtcgta aatactgtga tcccgtccat aggtgtagtc gatcagctgg
     2881 tccgggacgg ccagctgccc ggggccaggg ccgggtgcaa tgacccccac cgtgttgagt
     2941 gcgagaacgt ttttcacgtc caggagcttc agggccgcca ggttggcgcg gtagttgatg
     3001 cggtgcgggg ggatgtcgtg ccgatccccg tggcgcggca ggaaccatat gcggcggccg
     3061 ctgaacttta tttcccggac cgctgcagag ggcgctccgt aggccgttac cagccgccgc
     3121 cccggcgaca ggctggcgaa atcgctgaag ccggagcctg ccaggatggc aaatgacgcg
     3181 gctgccgggc cttgtgccgg ttcaggcatt cagttcgagt tccggccgtt cggaccagcg
     3241 gccgaggcgc tgggctgccg cctgccactc gtcgctggcc agcagtgttt cgcgaagcaa
     3301 ctggccggtg ccgtgcagcc gcgcgagccg caccagtgac agcggattcg agtagtcgcg
     3361 cagggtcgca acggtcgccc gggctgccgg gcggtcattg cagatcagca ccatgtcgca
     3421 acccgcctcc agcgcgagtg ccgcgcgctt cggcatcgga ccgaactcgg cggccgcctt
     3481 catgctgagg tcgtcgcaaa acaccgcgcc gtcgaagttg agctgtgtcc gcaactggtt
     3541 tccgatccag tatcgtgaga atcctgcagg cagcgggtca gtctccacgt acacgacgtg
     3601 tgcaagcatc actcccgcca
//
LOCUS       DASUOM010000541         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1372026,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000541 DASUOM010000000
VERSION     DASUOM010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372026"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="VFY27_11295"
     CDS             complement(<1..1857)
                     /locus_tag="VFY27_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEX5766144.1"
                     /translation="MQLLQSFLRHVVLFFATVALPAMGIAAEVRSPDGRIVFTVEVDD
                     SGGPYYRVDYRGETVVARSRLGMRFEAQHGFDAGFRIAGTSESSNDSTWEQPWGERRT
                     VVDRHNELLVEFAADEGSQRKFALRVRAFDDGVGFRYEVPEQRGYADVNIVDELTEFT
                     LQPDATAWWIPARRYNRYEYLYNTTRLTEIQNAHTPMTLRLPSGTHVSIHEAALVDYA
                     AFVLDQQRDGVFQTDLTPWSDGIRVRTKTPFKSPWRTIQVAPDAVGLLNSDLILNLNE
                     PNKLGDVTWVEPGKYVGIWWGMHIGRNTWGSGPTHGATTEETKKYIDFAAEHDISGVL
                     VEGWNIGWDGDWYSNGDVFSFTEAYADFDLEELTAYAADKGVEIIGHHETSGSITNYE
                     KQMSDAYRLYESLGVRKVKTGYVADAGDLKRLDDKGIPRYEWHDGQFSVNHHLRALTE
                     AAKHRISLNPHEPVKDTGLRRTYPNWLSREGARGQEYNAWGEPPNPPEHTAILPYTRM
                     LSGPMDFTPGVFDLTFKGVDSPFRVKTTLAKQLALYVVLYSPIQMAADLPENYEQRPD
                     AFRFIVDVPTDWEQSVALAGEVGDFVAFARKERDGADWYLGALTDENPRSLSL"
     gene            2089..3945
                     /locus_tag="VFY27_11300"
     CDS             2089..3945
                     /locus_tag="VFY27_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX5766145.1"
                     /translation="MGISPARLLLPLLALLFASGAGAQWEIQHLEPPFWWTGMQSPTL
                     ELMVHGLNIAELEPRLDYPGVGIADVHRTGNPNYLFLELELAGNVASGAFVIDFRRDG
                     ETELTYRYELRPRETGSADRKGFDASDVIYLVTPDRFANADASNDEVDGLVDGLDRGD
                     PHGRHGGDLQGIIDHLDYIAGMGYTQIWLNPVLQNDQPESSYHGYATTDFYQVDARFG
                     DNELYRELSAEAASRGIGLILDFIPNHSGSEHWWMKDLPGADWINHGSEFVATTHKRE
                     SLFDPNGAESDRLRFSDGWFVSAMPDLNQRNPRLARYLVQNSIWWVEFAGLSGIRVDT
                     WPYSDKEFLAEWTRRMLEEYPRLNIVGEEWVTNPAIVSYWQRGTLRQDGYESWLPSLM
                     DFPLHDAAIRGLKEEEGWGLGLDRIYGMLANDFLYGDPHNLVIFPDNHDMSRIYTQLD
                     ERIELYKMALAFFLTTRGIPQLYYGDEILMANPGTDAHGVIRGDFPGGWPGDDTNGFT
                     GEGLTELQREAQALTKTLLNWRKSASAIHGGKLTQFAPEGSTYTYFRHDDRQKVMVVI
                     NKAPGDHPLDVARFAEIIGDAARGVDVVTGEEYVLDGTFKAPANAALVLELR"
     gene            4100..>4455
                     /locus_tag="VFY27_11305"
     CDS             4100..>4455
                     /locus_tag="VFY27_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766146.1"
                     /translation="MTTTTRGFILQATYRVAGGPGDERHPVVHLYGRLEDGSSFLVRD
                     DRQRPHFYIRAADAEAAKALGAPQPETLDRRTFDGRPVCRIDVPIPSDVPPLRDRLHA
                     ANIDTFEADVRFASRYL"
BASE COUNT          829 a         1407 c         1364 g          855 t
ORIGIN      
        1 cagcgacagg ctgcgtgggt tctcgtccgt cagcgcgccg aggtaccagt ccgctccgtc
       61 tcgctccttg cgcgcgaacg cgacgaaatc gccgacctcg ccggccaaag cgacgctctg
      121 ctcccagtcc gtggggacat cgacgatgaa ccggaacgca tccggccgct gctcgtaatt
      181 ttcgggaaga tcggccgcca tctgtatcgg gctgtacagc actacataaa gagccagctg
      241 tttcgccagc gtggtcttta ccctgaaagg agaatcgacg cccttgaacg tcaggtcgaa
      301 gaccccggga gtaaaatcca ttgggcccga cagcatccgc gtgtacggca ggatcgccgt
      361 gtgctccggt ggattgggcg gctcgcccca cgcgttgtat tcctgcccgc gcgcaccttc
      421 gcgcgaaagc cagttgggat aagtgcggcg aagccctgtg tctttgaccg gttcgtgcgg
      481 attcaggctg atcctgtgtt tcgcagcttc cgtcagtgcg cgcaggtgat ggttcacgct
      541 gaactgtccg tcgtgccatt cgtagcgcgg gatgcccttg tcgtcgagtc gcttcagatc
      601 gccggcgtcg gcaacatatc cggtcttgac cttgcgcacg cccagcgatt cgtaaagccg
      661 gtaggcatcg ctcatctgct tctcgtagtt cgtgatgctg cccgaggttt catgatgccc
      721 gatgatttcg acgcctttgt cggcggcata ggccgtcagt tcctccaggt cgaaatccgc
      781 ataagcttcg gtaaaactga acacgtcgcc gttcgaatac cagtcgccgt cccagccgat
      841 gttccagccc tcgaccagta cgccgctgat gtcgtgctcg gctgcgaagt ctatgtactt
      901 cttcgtctcc tccgtcgttg cgccgtgcgt cggccccgag ccccaggtgt tccggccgat
      961 gtgcatgccc caccagatgc cgacgtactt gccgggctct acccaggtga cgtcgccaag
     1021 cttgttcggc tcgttcaggt tcaggatcag gtcggagttc agcaggccga cagcgtcagg
     1081 cgcgacctgg attgtccgcc acggcgactt gaaaggcgtt ttcgtcctga cccggatacc
     1141 atcggaccat ggggtcaggt cggtctggaa aacgccgtcc cgctgctggt cgagcacgaa
     1201 ggcggcgtaa tcgacgagcg ccgcttcgtg tatcgaaaca tgggtgccgg aaggcaggcg
     1261 cagggtcatc ggcgtgtgcg cgttctggat ctcggtcagg cgggtcgtgt tgtaaaggta
     1321 ttcgtagcgg ttgtagcgcc gcgccggaat ccaccaggcg gtcgcatcgg gctgcagcgt
     1381 aaattcggtc agctcgtcca cgatgttgac atccgcatag ccgcgctgtt ccggcacctc
     1441 gtagcgaaac ccgaccccgt cgtcgaaggc gcgcactcgc aacgcaaact tgcgctgcga
     1501 accttcgtcc gcggcaaact cgaccagcag ttcgttatgc cggtcgacca ctgtccttcg
     1561 ctcgccccag ggttgctccc acgtactgtc gttgctgctt tcgctcgtgc cggcgatgcg
     1621 aaagccggca tcgaagccat gctgcgcttc gaaacgcatg ccgagacggg agcgggccac
     1681 gaccgtctcg ccgcgatagt cgaccctgta gtacggcccg ccgctgtcgt ccacctcgac
     1741 tgtaaaaacg atacggccat cgggcgaacg tacttccgcc gcgatgccca tagcgggcaa
     1801 cgcgacagtc gcgaagaaca ggacaacatg cctgaggaag gattgaagta gctgcatgcg
     1861 gtggtctctg ttgtttgtag gagcccgcca tgcggggcga gttcgccgca ctcgctcgca
     1921 tggcgagctc ctacagcgtt tgttcgacgc gcgctagtct acaacggcga ctgaggatcc
     1981 ctccttgtat acgtattcat cgtcataaag ccctcatgaa tggcagcatt caggtgccat
     2041 ccggtaatct gcgcgatggc atcatccggt gctggggaga tgacgttaat gggaatatca
     2101 ccagcgaggc tcctgttgcc gctgcttgcg ctcctgttcg catccggggc gggcgcccag
     2161 tgggaaatcc agcacctcga gccgccgttc tggtggaccg gcatgcagag cccgacgctc
     2221 gaactcatgg tgcacggctt gaacatcgcg gagctcgagc cgaggctcga ctatcccggt
     2281 gtcgggattg cagacgtcca caggaccggc aatccgaact atctgttcct cgagctggag
     2341 cttgccggca acgttgcgtc cggcgccttc gtaattgact tccggcgtga cggagaaacg
     2401 gagctcacgt atcggtacga gcttcggcct cgcgaaaccg gttccgccga cagaaaaggc
     2461 ttcgatgctt cggacgtcat atacctggtc acgccagacc gctttgccaa cgcggatgct
     2521 tcgaacgatg aagtggacgg cctcgtggat ggactggacc gcggcgaccc gcatggccgg
     2581 cacggcggcg acctgcaggg gatcatcgat cacctcgact atatcgccgg catgggctac
     2641 acacagatct ggctcaaccc cgtgctgcag aacgaccagc cggaatcctc ctatcacgga
     2701 tatgcaacca ccgattttta ccaggtggac gcccgcttcg gcgacaacga gctctacagg
     2761 gagctttcag ccgaagcggc gagccgcggc atcggcctga tcctggattt catcccgaac
     2821 cattccggct ccgaacactg gtggatgaag gacctgccgg gcgcggactg gatcaatcac
     2881 ggtagcgagt tcgtcgcgac cactcacaag cgcgagagcc tgttcgatcc gaacggcgcc
     2941 gaaagtgaca ggttgcgttt cagcgacggc tggttcgtct ccgcgatgcc ggacctgaac
     3001 cagcgcaacc cgaggctcgc tcgatacctg gtgcagaaca gcatctggtg ggtcgagttc
     3061 gccggccttt ccggcatccg cgtcgacaca tggccgtact cggacaagga atttctcgct
     3121 gaatggacgc ggcggatgct ggaggagtac ccgcgcctca acatagtggg cgaggagtgg
     3181 gttacgaatc ccgccatcgt ctcctactgg cagcgaggca cactgcggca ggacggttac
     3241 gagtcctggc taccgagtct catggatttc cccttgcacg atgcggcaat tcgcgggctg
     3301 aaggaagagg aaggctgggg cttaggcctc gaccggatct acggcatgct ggcgaacgac
     3361 ttcctatacg gcgacccgca caatctcgtg atctttcccg acaaccatga catgagccgc
     3421 atctacacgc agctcgacga acggatcgag ctgtacaaga tggcgctcgc gttcttcctg
     3481 acgacacgcg gcattccgca gctttactac ggcgacgaga tcctgatggc caaccccggc
     3541 acggatgcgc acggcgtgat tcgcggcgat tttcccggcg gctggcccgg tgacgatacg
     3601 aacggattca cgggtgaggg cttgacggag ctgcaacggg aggcgcaggc gctgacgaaa
     3661 acgcttctga actggcgcaa gtccgcgagc gccatacacg gcgggaagct gacgcagttt
     3721 gcgccggaag gaagcaccta tacctacttc cggcacgatg accggcagaa ggtaatggtg
     3781 gtaatcaaca aggctcccgg ggatcacccg ctcgacgtgg cgcgattcgc cgaaatcatc
     3841 ggcgatgccg cgcgaggcgt ggacgtcgtg acaggcgagg aatacgtgct cgacggaacc
     3901 ttcaaagcgc cagcgaacgc agcgctcgtc ctcgagttgc gatgaactct gttcgcgcgc
     3961 tgtaggagcc cgccacgcgg gcgagttcgc tgacgtctgg tcgaactcgc ccgcatggcg
     4021 ggctcctaca gtgcatgtcg actgctacaa ggtggaaaag cggcggaccc ctcgcccgca
     4081 tttcgtgcaa actacccccg tgaccaccac gactcgcggc ttcattctcc aggcgacgta
     4141 tcgtgtcgcc ggcggccccg gggacgagcg tcaccccgtc gtgcatcttt acgggcggct
     4201 ggaagacgga agcagctttc tcgttcgtga cgaccggcag cgaccgcatt tctacatccg
     4261 ggccgccgac gcagaagccg ccaaggcact cggtgcgccg cagccggaga ccctcgacag
     4321 gcgcacgttc gacggccggc ccgtctgccg catcgacgtc ccgattcctt ccgacgtgcc
     4381 gccgctccgg gatcgactgc acgcggcaaa catcgacacc ttcgaggcgg acgttcgctt
     4441 cgcttcgcgt tacct
//
LOCUS       DASUOM010000542         5596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1517648,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000542 DASUOM010000000
VERSION     DASUOM010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517648"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFY27_11310"
     CDS             <1..878
                     /locus_tag="VFY27_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5766147.1"
                     /translation="FWSLAWIVSSFVFAGWLWWYLGGTGPDAEAQFIARDKMLQFLTG
                     YGIEKALAVDNVFVFLMVFTYFGVPPEYQKRVLMIGILGALFLRALLILVGAWIIGQF
                     HWVLYLFGAFLVYTGIRMWYAGARKPDLESNATLKWLRQRMHIAPDFDGEKFLTIENG
                     QRALTPLALVILLIGVVDVLFAVDSIPAIFAITTDPFIVLTSNVFAVLGLRALYFLMV
                     NMHERFHLLSYGLSFLLVFIGGKLLLMDIVEIPVGLSLLVTFSILIFAIVLSAYVPRK
                     DPDGSARVAPGELND"
     gene            complement(909..3365)
                     /locus_tag="VFY27_11315"
     CDS             complement(909..3365)
                     /locus_tag="VFY27_11315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5766148.1"
                     /translation="MHQKSKYGAIAISIMLLAWQDAAAGLHLRNAVVERLDNGLTVIL
                     LEDRNFPVVSTQMLYRVGARDESYGHTGLAHFLEHMAFRDSENFPDTGLVSSIYAVGG
                     EWHGYTWTDETTYFATVPKEELGLLLSIEADRMSRLRISPDDMEAERGAVLAEMHMYE
                     NYPTSMLLDAVLFTSFFAHPYRNNTIGWESDIEALEHPEVVAFYERHYHPANAVLAIV
                     GDFDRRQVLERVETLFGKIEAKAPTRWPHTVEPVQEGERRVRLHAETDVRRFMIAWRA
                     PSANHEDFAAFLVLQELLGAGSGVNFRQNDWGTPLDDGALLEGLADEVTTWYPPSAQD
                     YIFIVGGTIPPEDRESRLEEDLEERLAGVRRRVTAPADLQAAIDRVIEELEYDVETTE
                     DAAHQLAFFDGLGALDTLLRLPQRVRAVTGTDVQRVARRWLLPERRTIGWHVPGQDTE
                     KLPIMEAPPSQAEKNNAPDAPPGPPDREPVPGPEMRELRGGVPVILQQSDLSQAAFLQ
                     VVLPGSDVAGAGVRRSEPVAGYSSVAWRFVPEDLRKTVARAREAVEAARVGVDLKRDA
                     PTDPETAIEQVFASRMSGNEPAQGTTGVSPALVVVSGDLATERVFELLDEAFGGMPAG
                     QRVRSRPSANVNDRHPIEVHLERPVAQAQLGYIVAVPGPAEEASWAHRILLYILSHDY
                     EGRLGTEAISNRGLAYYIDSRYRSDGANAWITLAVGVDPAKLPDLKNLLQAELERLQK
                     EPPSHAEIEEAKRHFTGRARSASQSNAEISSALAEQWLWYGKLLTADDLERRLRRVDR
                     EAVLDAVSSFIDGATVVVTE"
     gene            3532..4941
                     /locus_tag="VFY27_11320"
     CDS             3532..4941
                     /locus_tag="VFY27_11320"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5766149.1"
                     /translation="MGSSAGVMAKPMVAAVIALCLAQPAPGQENAHETLAREILAELI
                     AANTAPSGGNDMRQAVAGLVTRLKNAGFSDDDMTIVAETPTLPNLVIRYRGNSRGTRP
                     LLMMAHLDVVEALPKDWTVEPFFLTEKDGYFYGRGTTDNKAGAAIMVANFIRMKREGY
                     VPNRDLIIMLTADEETTGAGAQLLVNEHRDLVDAEFALNTDGGMIILEGEDPQAFIMQ
                     TSEKVYVSFTLEATDPGGHSSVPRANNPISRLSKTLVDIGEFQFPADLNETSRAFFRL
                     WEPMAPEEARPVIRSILENPGDPQLSAKLVEHPYFNALARTTCVATQLEGGHAENALP
                     QTARATVNCRVLPQSSAEETHNVLTAMAAKNGVSVTQIAPALASPASPLDPSIVDPVE
                     HVADDMWPGITVIPEMSTGATDGLFMRNAGIPVYGVSAIAEDPDDFRAHGQDERIRVQ
                     SYYEALEYWYRLVKLLSRP"
     gene            4974..>5596
                     /locus_tag="VFY27_11325"
     CDS             4974..>5596
                     /locus_tag="VFY27_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766150.1"
                     /translation="MSPITPSRAIPALILAVLALDPASPAIAVETVQGTIAAITAEDW
                     RVSGLSFSVDAGEPLNGSLRIASLELPSIELSLADVLVECRRIELSAVEFGCLDAEYT
                     LDLPDVGQLEFPGEAVYERRSGATRFVLREIPLAQGKITLRGTATADAVEIEYSGEML
                     KLDGLTELAQRFTPGGIALTASGTGNLSGAARLRDGSVTNASMRAEVS"
BASE COUNT          988 a         1815 c         1711 g         1082 t
ORIGIN      
        1 gcttctggtc actcgcgtgg attgtctcga gcttcgtgtt cgccggctgg ctgtggtggt
       61 acctcggcgg aaccggaccg gacgcagagg cgcaattcat cgcgcgcgac aagatgcttc
      121 agttcctcac cggctacggc atcgagaagg cgctcgctgt cgacaacgtg ttcgtcttcc
      181 tgatggtatt cacgtacttc ggagtgccac cggaatacca gaagcgcgtg ctcatgatcg
      241 gcatacttgg cgcgttgttc ctgcgcgccc tgctcatcct ggtcggggcc tggatcattg
      301 gccagtttca ctgggtgctg tacctgttcg gcgccttcct cgtctatacc gggatccgga
      361 tgtggtacgc cggcgcaagg aagccggacc tggagtcaaa tgcgacgctg aagtggctgc
      421 gacagcgcat gcacatcgca ccggatttcg acggcgagaa attcctgacg atcgagaacg
      481 gccagcgagc gttgacaccg ctcgcactcg tcatactgtt gatcggcgtc gtcgacgtat
      541 tgttcgcggt cgactcgatt cccgccatct tcgcgatcac gaccgatcct ttcatcgtgc
      601 tcacctcgaa cgtctttgcc gtactggggc ttcgcgcgct gtacttcctc atggtgaaca
      661 tgcacgagcg ctttcatctc ctgtcctacg ggctttcgtt cctgctggtg ttcatcggcg
      721 gcaagttgct cctgatggac atcgtcgaga tcccggtagg cctttccctg ctcgtcacct
      781 tctctattct tatcttcgcg atcgtcctgt ccgcctacgt cccgcgaaaa gaccctgatg
      841 gatccgcgcg agtcgcgccc ggagaactga acgattgacc cgcggcggtg actgcgaccg
      901 tcgtcgattc actcggtaac gacgacggta gctccatcga tgaacgagga gacggcatcc
      961 agcaccgcct ccctgtcgac gcgacggagg cggcgctcca ggtcgtcggc agtaaggagt
     1021 tttccgtacc agagccattg ctcggcgagt gccgacgata tctccgcatt gctttgcgaa
     1081 gccgagcggg cccgacccgt gaaatgccgc tttgcctcct cgatttccgc gtgcgagggc
     1141 ggctccttct gcaggcgctc gagttctgcc tgcagcaggt tcttcaggtc gggcagcttc
     1201 gccgggtcca cccccaccgc gagcgtaatc caggcatttg ccccatccga ccggtagcgg
     1261 ctgtcgatgt agtacgcgag gccgcggttc gaaatcgctt ctgtcccgag ccggccctcg
     1321 taatcatggc tcagtatgta gagtaatatc cggtgcgccc acgacgcctc ctcggcaggg
     1381 ccgggaaccg cgacgatgta gccgagctgg gcctgcgcca ccggccgctc gaggtgtacc
     1441 tcgatcgggt ggcggtcatt gacgttcgcg ctcggcctgc tgcggacgcg ctgccccgcc
     1501 ggcattccac cgaatgcttc atcaagcagc tcgaataccc gctccgtcgc gagatcgccg
     1561 ctcaccacga ccagggcggg cgaaactccc gtcgttccct gcgcaggctc gttcccgctc
     1621 attctggatg cgaacacctg ttcgatggcc gtctccgggt ctgttggtgc atcgcgcttt
     1681 aaatcaacgc caacccttgc ggcctccacg gcctcgcgtg ccctcgccac tgttttccgc
     1741 aggtcttcgg gaacgaagcg ccacgcaacc gaagaatatc ccgcgaccgg ttcgcttctt
     1801 cgcacgccgg ctccggcgac atcgctgccg ggcagcacca cttgcaggaa ggctgcctgc
     1861 gagagatccg actgctgcag gatcaccggg accccaccgc gaagttcgcg catctcgggt
     1921 cccggcaccg gttccctgtc cggcgggccc ggcggcgcat ccggcgcgtt gttcttttcg
     1981 gcttgcgaag gcggcgcttc cattatcggc agcttctccg tgtcctggcc gggtacgtgc
     2041 cacccgatcg tacggcgctc aggcagcaac caccgacgag cgacgcgctg cacgtccgta
     2101 ccggttacgg ctctcacgcg ttgcggcagt cgcagcaggg tgtccaatgc cccgaggccg
     2161 tcgaaaaacg cgagctggtg cgctgcgtct tcggtcgttt cgacgtcgta ttcgagttcc
     2221 tcgatcaccc tgtcgattgc tgcctgcagg tcggctggcg ccgtaacccg gcgacgtacg
     2281 ccggcgagcc gttcttcgag atcctcttcc agccgcgact ccctgtcctc gggcgggatc
     2341 gtgccgccga cgatgaagat gtaatcctgg gccgagggcg ggtaccaggt cgtcacctcg
     2401 tccgccaaac cttcgagcag cgctccgtcg tcgagcggcg tgccccagtc gttctggcgg
     2461 aagttcactc cggaacccgc gcccagcagt tcctgcagca ccaggaaagc ggcgaagtcc
     2521 tcatgattgg ccgacggcgc ccgccacgcg atcatgaagc gcctgacgtc cgtctcggcg
     2581 tgcagccgca cgcggcgctc gccttcctgc accggctcca ccgtgtgcgg ccatcgcgtg
     2641 ggcgctttgg cctcgatctt tccgaacagc gtctcaacgc gctcgagcac ctggcggcga
     2701 tcgaaatcgc cgacgatcgc gagcacggca ttcgcagggt ggtagtggcg ctcgtaaaag
     2761 gcgaccacct ccgggtgttc gagcgcttcg atatcgcttt cccagccgat cgtattattg
     2821 cgatagggat gagcgaagaa ggatgtaaac aagactgcgt cgagcagcat ggaagtcggg
     2881 taattttcgt acatgtgcat ctccgccagc acggcgccgc gctcggcttc catgtcgtcc
     2941 ggcgaaatgc gcaggcgcga catccggtcg gcctcgatgg agagcagcag gcccagttcc
     3001 tctttcggca cggtggcgaa gtacgtcgtc tcgtcggtcc atgtatagcc atgccattcg
     3061 ccgccgacgg cgtagatgga actgacgagc cccgtatcgg gaaagttttc cgaatcgcga
     3121 aacgccatgt gctcgaggaa atgcgcgagg ccggtgtggc cgtaagattc atcgcgcgca
     3181 cccacgcggt acagcatctg agtgctcacc accggaaaat tccgatcctc gagcaggatc
     3241 accgtcagcc cgttgtccag gcgctcgacg acagcattgc gcaggtgaag gccggccgcc
     3301 gcatcctgcc atgcaagcaa catgatcgat atggcgatcg ccccgtactt cgacttctgg
     3361 tgcaccgaag cctcccgttg tccgatgtac tgtcttgctt cctttgagga cgattctatc
     3421 tttcgccggt gccgacggtc aggcgcgctc catcatgtaa agcccacatc gtggacgggc
     3481 gaaggcggca ggccttacac tcacacgcca ctcaagggac aggatgatgc aatgggaagt
     3541 agtgcaggag tgatggcgaa accaatggtt gcggcggtga tcgcactgtg cctggcgcag
     3601 ccggcccccg gccaagagaa cgctcacgaa actttggcca gggagattct tgccgagctg
     3661 atcgccgcca acaccgctcc cagcggcggc aacgacatgc ggcaggcggt tgcgggactg
     3721 gtgacgcgcc tgaagaatgc aggattcagc gacgatgaca tgaccatcgt cgccgaaact
     3781 ccgactcttc ccaatcttgt gatccgctac cgcggcaact cccgcggcac ccggccgctg
     3841 ctcatgatgg cgcacctcga cgtggtcgag gcgttaccga aagactggac ggtcgagccg
     3901 ttcttcctga ccgagaagga tggttacttc tacgggcgcg gcaccaccga caacaaggcc
     3961 ggcgccgcga tcatggtcgc aaacttcatc cgcatgaaac gcgaggggta tgtgccgaat
     4021 cgcgacctca tcatcatgct gactgcagac gaggagacta ccggcgccgg cgcgcagctc
     4081 ctggtcaacg agcaccggga tctcgtagac gcggaattcg ccctcaacac cgacggcggc
     4141 atgatcattc tcgaaggcga agacccgcag gccttcatca tgcagacgag tgagaaggtt
     4201 tacgtgagtt ttacgctgga ggcgaccgat ccaggcggtc acagctccgt gccgcgcgcc
     4261 aacaacccga tatcgaggct ctcgaagacg ctcgtcgaca ttggagaatt ccagttcccg
     4321 gcagacctga acgaaacctc gcgggcgttc tttcgcctgt gggagccgat ggccccggag
     4381 gaggcccggc cggtgatcag gagtatcctg gaaaaccccg gcgatccgca gctgtcggcg
     4441 aagctcgtag agcaccctta ttttaacgcg cttgcgcgaa ccacctgcgt tgcgacgcaa
     4501 ctcgagggtg ggcacgcgga gaatgcattg ccgcaaaccg ccagggcaac cgtcaattgc
     4561 cgcgtactgc cacagtcgtc cgccgaagag acgcacaacg tgctgaccgc gatggcggcg
     4621 aagaacggcg tctccgtcac gcaaatcgct cccgcgctgg ccagtcccgc atcgccgctc
     4681 gacccctcga tcgtcgatcc ggtcgagcac gtggcggacg acatgtggcc cggcatcacc
     4741 gtaattcccg agatgagcac cggcgcgacc gacggacttt tcatgcgtaa tgccggcata
     4801 cccgtatacg gagtctccgc catcgccgaa gacccggacg acttccgcgc ccatggccag
     4861 gacgagcgca ttcgggtgca gtcgtactac gaggcgctcg aatactggta ccggttggtg
     4921 aagctcctgt cccgaccttg acccggagcc gggatcggat gccggaggcg cgtgtgagtc
     4981 caatcacacc ctcacgtgcc atcccggcac ttatccttgc cgtcctggcc cttgaccctg
     5041 catcgcctgc cattgcggtg gaaacggtgc agggcacgat cgcggcgatt acggcggagg
     5101 actggcgggt ttcgggactt tctttttcgg tcgatgccgg tgagccactg aatggcagct
     5161 tgcgcatcgc ctcgctggaa ctgccgtcga tagaactttc ccttgctgac gtgctggtcg
     5221 agtgccggcg cattgagctg tctgccgtcg agttcgggtg tctcgatgcc gagtacacgc
     5281 tggatctgcc ggacgttggg caactcgaat ttccgggcga ggcggtgtac gagcggcgaa
     5341 gtggcgctac ccggtttgtc ctgcgcgaga ttcccctggc gcaggggaag atcacgctcc
     5401 gcggcaccgc tacggcggac gctgtcgaga tcgagtactc cggtgaaatg ctcaagctgg
     5461 atgggctcac ggaactcgca cagcgcttta cccccggtgg catcgcgctg acagcatcgg
     5521 gaaccggcaa cctgagcgga gcagcgcgcc tgcgggatgg aagcgtgacc aatgcgtcca
     5581 tgagggcgga ggtctc
//
LOCUS       DASUOM010000543         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_792804,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000543 DASUOM010000000
VERSION     DASUOM010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792804"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VFY27_11330"
     CDS             <1..2116
                     /locus_tag="VFY27_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5766151.1"
                     /translation="AGEVVEVFRRTLETGEPHHEPEFAEYRTDRNLTEYYDWRTQRIV
                     LHDGSYGIVCYFRDVSEEVTARKRVAESEFRYRTLFESIDVGFCIGEIILDDGGNAID
                     YRFLVTNPAFETHTGLVDARGKTIREIVPDIEDRWIELCARVAMTGEQTRFVDHARAI
                     DRWFEVDAFRTGKPEQRRVAMLLSDVSERKRAEQALRESEQRFRTMADSAPALIWVSD
                     TTGDCTYFNRQWLEFTGRPLEAELGAGWMESVHPDDREQTEEAYGSASAVRGPVRTEF
                     RLRRHDGVYRWLVEEGVPRHLPDGQFAGYVGVCIDITQRMQWEKALRETDRRKDEFLS
                     TLSHELRNPLAAVSTATNVLMNSRDNADRVGEMAAIIERQSAQLVRLIDDLLDVGRIS
                     RGKVKLERRHIDVADVVRQVIVDCGSVCDQKRLGLVVDLPEQPVLVDADPVRVAQVVN
                     NLLDNACKFTPSGGRVSVSVGQESGEAVINVADTGIGMSREQLPRIFEMFTQVEDAPG
                     DRTGGLGIGLSLAQSIMELHGGTIEAHSEGREKGSKFALRLPVSDSDAPGDDVAETGE
                     AEAGQPVLKRIIAADDNLDTLHAVALLLRMKGHEVETAVDGVDALKKVQAHPPDVALL
                     DIGMPGMDGYEVARRIRREPWGQNVLLVATTGWGAQRDKQQAQAAGFDAHLTKPVDLD
                     ALERLIAAPQKSTVGNRDVPLH"
     gene            2183..>2918
                     /locus_tag="VFY27_11335"
     CDS             2183..>2918
                     /locus_tag="VFY27_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766152.1"
                     /translation="MTSRLPSSIVPANFITILPEVAMLIARPARLLKGLYLLLLMLAA
                     CSGSPDEEEALASADGILRFIPADAPYVFAATEPLPEEAYRKLAPYVETIVQTYSSIL
                     LAAINQQPDDSSESHLDEETAKRLSSFIQQIAGMITEDGIPEAGIDRDSTGAVYGVGL
                     LPVLRITLSDGSLFESTMTRMESEAGERMSVADLDGHPYRYAGNDEARVILAVIDDHL
                     VATVVPANLPEASLKAVLGIELPGRNI"
BASE COUNT          603 a          823 c          962 g          530 t
ORIGIN      
        1 ggcaggggaa gtcgtggaag tctttcgacg tacgctcgag accggggagc cccatcacga
       61 gccggaattt gccgagtacc ggactgaccg taacctgacc gagtactatg actggcgcac
      121 ccagaggatc gtgctgcacg atggctctta cgggatcgtt tgttattttc gcgacgtctc
      181 cgaggaggtc accgcccgga agcgggtcgc ggaatcagag ttccgttacc gcacgctttt
      241 tgagtcgatt gacgttggct tctgcatcgg ggagatcatc ctcgacgacg gcggcaacgc
      301 gatcgattat cgcttcctgg tgaccaaccc cgccttcgaa acacacacag gcctggtgga
      361 tgccaggggc aagacgatcc gcgaaatcgt gccggatata gaagatcgtt ggatcgaact
      421 gtgcgcgcgc gtagctatga ccggagaaca gacgcggttc gtcgaccacg cgagggcaat
      481 cgaccgctgg ttcgaagtcg acgcattccg gaccggaaag ccggagcagc ggcgggtagc
      541 gatgctgttg tcggacgtca gcgaacggaa gcgggccgag caggccttgc gggaaagcga
      601 acagcgtttt cgcaccatgg ctgacagcgc accggcactc atatgggtat ccgacacgac
      661 cggcgactgc acgtatttca accgccaatg gctggagttt acggggaggc cgcttgaagc
      721 ggaactgggc gccggatgga tggagtccgt acaccccgac gaccgtgagc agacggaaga
      781 agcttatggc tccgcatctg ccgtccgcgg acccgtccgg accgaatttc gcctgcggcg
      841 ccacgacggg gtgtaccggt ggctggtgga agagggcgtt ccgcggcacc ttccggatgg
      901 gcagtttgca gggtacgtgg gtgtgtgtat cgacataacg cagcgcatgc aatgggaaaa
      961 agcgcttcgt gagacggacc ggcgcaagga cgaattcctt tccactttgt cacacgagct
     1021 gaggaatccg ctggcggcag tcagcacagc cacgaatgtg ctcatgaatt cgcgcgacaa
     1081 tgcagaccgg gtgggcgaga tggcggccat catcgaacgg cagtcggccc agctcgtacg
     1141 actgatcgat gatcttctcg atgtcggccg gatctcgcgt ggaaaggtaa agctcgagcg
     1201 gcggcacatc gatgttgcag acgttgttcg ccaggtgatc gtcgattgcg gctcagtgtg
     1261 cgatcagaaa cggctgggac tcgtggtgga ccttccggag caacccgtgc tggttgacgc
     1321 cgaccccgta cgcgtggccc aggtcgtaaa taacctgctc gacaatgcgt gcaagtttac
     1381 gccgtcaggc ggccgggtaa gtgtttcggt cgggcaggaa agcggcgagg ccgtcatcaa
     1441 cgtcgcagat accggcatcg gcatgtcgcg cgagcagctc ccgcgcatct ttgaaatgtt
     1501 tacccaggtc gaggatgcac cgggagatcg taccggcggt ttgggcattg gtctttcgct
     1561 ggcgcaatcg atcatggaac tgcacggcgg cactatcgag gcgcacagcg aaggtcgcga
     1621 aaaaggcagc aagtttgcgc tgcgacttcc ggtctccgac agcgatgcac cgggtgacga
     1681 cgtcgcggag acaggcgaag cggaagcggg acagcccgtg ctgaagcgca tcatcgcggc
     1741 agacgacaat ctcgatacgc tgcacgccgt cgctctcctg ctacgaatga aaggccatga
     1801 agtggaaacg gctgtcgacg gggtcgacgc gctgaaaaag gtgcaggcgc atccgccgga
     1861 cgtggcgctg ctggacatcg ggatgcccgg catggacggg tacgaagttg cgaggcgcat
     1921 tcgccgggag ccgtggggcc agaacgtgct tctggtggca acgacaggct ggggcgcgca
     1981 gcgcgacaag cagcaggcgc aggcggctgg attcgatgcg cacctgacga agccggtcga
     2041 cctggatgcg ctcgagcgat tgatcgcggc accacagaag agcacggtcg gtaaccggga
     2101 cgtcccgctt cactagggag aggctcccta gcgaagggtt cttcccggga gggctacagc
     2161 acgatatatg tgaccagtga cgatgacgtc tcgactgccg tcgagtatcg tacccgccaa
     2221 tttcatcacc atcctgccgg aagtagccat gctcatcgcg agacctgccc gcctgctgaa
     2281 aggcctgtac ctgttgctgc tgatgcttgc ggcgtgttcc ggatcgccgg acgaagagga
     2341 ggccctggca tccgcggacg gcatcctgcg cttcataccg gcagatgcgc cttatgtgtt
     2401 tgccgcgaca gagccgctgc cggaagaggc gtatcgaaaa ctggcccctt acgtcgagac
     2461 gatagtgcag acgtacagct cgattctgct cgcagcaatc aaccagcagc cggacgattc
     2521 ttccgaatcg catcttgacg aggaaacggc aaagcgcttg tcgtcgttca ttcagcagat
     2581 agccggcatg attacggagg acggtatacc ggaggcgggc atcgatcggg actcaacagg
     2641 cgccgtgtac ggtgttggtt tgttgccggt ccttcgcatt acgctcagcg atggcagcct
     2701 gttcgaatcg accatgacca ggatggaaag cgaggctggc gagagaatga gcgtcgctga
     2761 tctcgacggc cacccatacc gctatgccgg gaatgacgag gcccgcgtga tactggccgt
     2821 catagacgac cacctggtgg caaccgtcgt gccggctaat ctcccagagg cttcgttgaa
     2881 ggcggttctc ggtatcgaac ttcccggcag gaatatcg
//
LOCUS       DASUOM010000544         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_270579,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000544 DASUOM010000000
VERSION     DASUOM010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270579"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFY27_11340"
     CDS             complement(<1..899)
                     /locus_tag="VFY27_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5766153.1"
                     /translation="MNAKTRNDGTPRRTPILHFLAVLISALQGVATTTPAHADGTAVL
                     LELEGALGVATAEYIVGGIEDAEEAGANIVIIRMDTPGGLVEPMRDIVQKILASTVPV
                     ATYISPGGARGDSAGTYILFASHIAAMAPTTHVGAATPVSLGGGDPPDSSPPFGERPG
                     GDEDPAGTEGEEGAEGEGATDEAGDDEEHEPPVPGSSMERKVLNDAIAYIRGLAEAHG
                     RNADWAEKAVREAATLTAEGALEQNVIDLIANNQVELLAAIHGREVTVNNETVTLDTE
                     GLEIEVVEPSWRIKILTTISNPE"
     gene            1040..1522
                     /locus_tag="VFY27_11345"
     CDS             1040..1522
                     /locus_tag="VFY27_11345"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX5766154.1"
                     /translation="MFIRFITTGGTIDKVYFDAISQFEVGDSQLKHILGDGLVDFEYD
                     IVALFHKDSLELSEEDRAVIRQHIEEDDARLIVVTHGTDTMVQTATALHGIPGKSIVL
                     TGSLTPARFKSTDAIFNVGMAVAAVQIVQPGVYIVMNGQVFPAGSVRKNREANRFERC
                     "
     gene            complement(1609..>2766)
                     /locus_tag="VFY27_11350"
     CDS             complement(1609..>2766)
                     /locus_tag="VFY27_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5766155.1"
                     /translation="DFAVGQSRMLYGKTMPSERPMHRMYEQWHPLGIVGTISAFNFPV
                     AVWSWNACIAAICGNVNIWKPSPKTALCGVAVQKICNAALADAGFPEIFHLFNDGDNR
                     LAQKFVDDPRIDLLSFTGSTAVGRKVGERVAARLGKSLLELGGNNAIIVDEFANLNLA
                     VPAIVFGAVGTAGQRCTTTRRVLVHASRMKELQERLVKAYGQVRIGDPLDAKTLMGPL
                     IDEAAVERVRKAAEAVRRAGGEILCGGKVLDRPGNFIEPIIAVAKNDWDVVQTETFGP
                     ILYLVPVNSLEEAVSLHNGVAQGLSSSIFTDRLQHAEYFLSHLGSDCGIANVNIGTSG
                     AEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWGAKLPLAQGINFGL"
BASE COUNT          484 a          881 c          852 g          549 t
ORIGIN      
        1 atttccggat tcgatatggt ggtcaggatc ttgatgcgcc agctcggctc gaccacctcg
       61 atctccaggc cttccgtgtc cagcgtcacc gtctcgttgt tgacggtgac ctcccgcccg
      121 tgaatagcgg cgaggagttc gacctggttg ttcgctatca agtcgatgac gttctgttcc
      181 agcgcacctt ctgccgtgag ggtcgccgcc tcgcgcaccg ccttttcagc ccagtcagca
      241 ttgcggccgt gagcctcggc caggccacgg atgtaggcga tggcgtcgtt caggactttg
      301 cgctccatgg agctcccggg aacgggcggt tcatgctcct cgtcgtcccc ggcctcgtcg
      361 gtagctcctt cgccttcggc gccttcctcg ccttccgtgc cggcggggtc ttcgtcacct
      421 cccgggcgct cgccgaatgg tggcgaggaa tcgggagggt cgccgccgcc gagagaaacg
      481 ggtgttgccg cgccgacatg cgtcgtcggc gccatcgccg cgatatggct ggcaaagagt
      541 atgtaggtgc cggcgctatc gccgcgagcg cccccgggtg atatataggt tgcgaccggt
      601 acggtggagg cgaggatctt ctgcacgatg tcgcgcatgg gttcgaccag gccgcccggg
      661 gtatccattc ggatgatgac gatgttcgca ccggcttctt cggcgtcttc aattccgcca
      721 acgatgtatt cggccgtcgc cacgcccagc gccccttcga gttcaagcag gaccgcagtg
      781 ccgtccgcgt gagcgggagt ggtggttgca actccttgaa gggccgagat cagcacggcc
      841 aggaaatgca ggatgggagt ccttcgcggc gtaccgtcat tccgggtttt tgcgttcacg
      901 cgtcaatgat agcagggcgc gcggccccgg ggacaagccg ccggcggcgt acggcaacat
      961 accggccctt ctgggccgcc cttctgatcg ctcgtcgtaa gtggcaaact tcgaggcccg
     1021 gcagtcgcgg cagagcacga tgttcatccg attcataacc accggcggca cgatcgacaa
     1081 ggtttatttc gatgctatca gccagttcga ggttggcgat tcgcaactga agcacatcct
     1141 cggcgacggg ctggtggact tcgaatacga catcgtcgcg ctattccaca aggacagcct
     1201 ggaactgagc gaagaagacc gggcagtcat ccggcagcat atcgaggaag acgatgcacg
     1261 gctgatcgtc gtcactcacg gtacggatac gatggtgcag acggctaccg cactgcatgg
     1321 catcccgggg aagtcgatcg tccttaccgg ctcccttacg cctgcgcgct tcaaaagcac
     1381 cgacgcaatc ttcaacgtcg gaatggccgt cgctgccgta cagatcgtgc agccgggcgt
     1441 ctacatcgtc atgaacggcc aggtctttcc agcaggaagc gtgcggaaga accgcgaagc
     1501 gaaccgcttc gagcgctgct gattctgcga agaacgtctt aggggcccgc catgcgggca
     1561 attccgttca cgtagatcgc acactcgccc gcgtggcggg ctcctgcgct acaagccgaa
     1621 gttgattccc tgcgcgagcg ggagtttggc gccccagttg atcgtgttgg tctggcggcg
     1681 catgtacgcc ttccatgcgt ccgagcctga ctcgcggccg ccgcccgttt ccttttcgcc
     1741 gccgaatgca ccgccgattt cggcgcctga ggtgccgatg ttcacgttcg cgatgccgca
     1801 atcggaaccg aggtgtgaca ggaagtactc cgcatgctgc aagcgatcgg tgaagatcga
     1861 tgacgacaag ccctgggcaa cgccattgtg cagtgatact gcttcctcga ggctgttcac
     1921 gggaaccagg tacaggatcg gcccgaaagt ctcggtctgc accacgtccc agtcgttctt
     1981 cgcgacggcg atgatcggct cgatgaagtt gccgggacga tccagaacct tgccgccgca
     2041 gaggatttcg ccaccggccc tgcgcactgc ctcggccgcc ttcctgactc tttctacggc
     2101 cgcctcgtcg atcagcgggc ccatcagcgt ctttgcgtca agcggatcgc cgatgcgaac
     2161 ctggccgtag gctttcacca gtcgctcctg cagttccttc atccgggatg catgcaccag
     2221 cacgcgccgc gtcgtagtgc agcgttgccc ggccgttccc acggcgccga aaacgatcgc
     2281 cggcaccgcg aggttcaggt ttgcgaactc gtcgacgata atcgcgttgt tgccgcccag
     2341 ctccagcagg cttttaccca ggcgcgcggc aacgcgttcg cccaccttgc gtcccaccgc
     2401 cgttgagccg gtaaacgaga gcaggtcgat acgcgggtcg tcgacaaact tctgcgcaag
     2461 cctgttgtcg ccgtcattga acaggtgaaa aatctcaggg aaacctgcat cggcaagtgc
     2521 cgcgttgcag atcttctgca ccgcgacgcc gcacaacgca gtctttggtg acggcttcca
     2581 gatattgacg ttgccgcaga tcgctgcgat gcaggcgttc cacgaccata ccgcgaccgg
     2641 gaagttgaac gcggaaatcg tgccgacgat accgaggggg tgccactgct cgtacatgcg
     2701 gtgcatcggc cgctccgagg gcatcgtctt gccgtagagc atccgggact ggcccacggc
     2761 aaagtc
//
LOCUS       DASUOM010000545         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1034604,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000545 DASUOM010000000
VERSION     DASUOM010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034604"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /gene="uxaC"
                     /locus_tag="VFY27_11355"
     CDS             complement(<1..1286)
                     /gene="uxaC"
                     /locus_tag="VFY27_11355"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX5766156.1"
                     /translation="MTQPLELHPDRLFPQEPRARDIARRLYALVADKPIISPHGHTDP
                     SWFAGNNSFSNAAELLLTPDHYLFRMLFSHGISLDAVGVSRVGGGTAAADPRACWRTF
                     AEHYYLFRGTPSRLWLDWVFVNVFGMKVQLDAGTADKYFDAIGEALAQEEFRPRSLFE
                     RFNIEVLATTESPLDELRHHAAIRDSGWQGRVITAYRPDPTTDPEFDGFADNLRRLGE
                     LAGEDTGSWRGYLSAQRVRREFFKSMGATSTDHGHATAQTADLDQDSARKLFDKVRTG
                     RHEKADAELFRAQMLTEMARMSIDDGLVMQIHPGSFRNHNRLIHRDYGRDKGFDIPVR
                     TNYVDGLKPLLDAVGLDERLSIILFTLDESTYSRELAPLAGVYPSLKLGPAWWFHDSP
                     AGMRRFRELTTDTAGFYNTVGFNDDTRAFLSIPARH"
     gene            complement(1402..2430)
                     /locus_tag="VFY27_11360"
     CDS             complement(1402..2430)
                     /locus_tag="VFY27_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX5766157.1"
                     /translation="MAQFVSFGEIMLRLKTPGNERFFQSPSFEATFGGGEANVAVALA
                     NFGLDAGFVTALPKNDIGDAAVRELRSFGVDTSSIIRSGDRLGIYFLETGANQRPSKV
                     IYDRAGSAIAEAKPGDFDWGRMLAGAKWLHITGITPALSQSAADLSFVCVKSAKAQGV
                     TVSCDFNYRGKLWKYGKSAIEVMSELVKSVDVGIANEEDCQKSLGVTADVDVSSGKLG
                     HAKYEALSEKMLETYPNLSVMAITLRESMSADRNGWSACLRDKSGFHLSRRYEITDIV
                     DRVGGGDSFASALIFGLNTYDSRQDALEFAVAASCLKHSISGDFNRVSVAEVRNLMGG
                     DASGRVQR"
     gene            complement(2499..3143)
                     /gene="eda"
                     /locus_tag="VFY27_11365"
     CDS             complement(2499..3143)
                     /gene="eda"
                     /locus_tag="VFY27_11365"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5766158.1"
                     /translation="MARTALIEERLATTGVVPLVQADDPEAALRTSEALLAGGLSIIE
                     VVLRTDDALNCLKAVSESLPEATVGAGTVLTKAQAEAAVAAGAAFIVSPGLDDGVVEV
                     ANAHGLPVFPGIATASELQHAFNLGLRVVKFFPAGVCGGVAMIRALSSAFREMKFMPT
                     GGVSAENLADYLAVPSVIACGGSWLTPTDAIRNRDYGRIRSLATEAVAIARSRS"
     gene            3268..>4130
                     /locus_tag="VFY27_11370"
     CDS             3268..>4130
                     /locus_tag="VFY27_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5766159.1"
                     /translation="MDTLAKQDPQLKVNLERLKSTLLELSRIGFNEADKGIYRVGFSE
                     ADMAARRWLKHLLESEDLCPRIDGAGNVYARLGDPDRPTVTLGSHLDTVPAGGMFDGA
                     LGVVAALEVLRTCREQQVDLVWPLELLATSEEEGRYGGMLGAQAIAGDLTPGIILNMH
                     DADGNMLSDAMTAAGMDPIGALDARRLPETMRAFLELHIEQGPVLEKLDKTIGVVDGI
                     SGVFKWIVRLIGKADHAGTAPMNMRSDAFMGLADFAHEIPRIIDENGTDRSRLTVGKV
                     ELKPGSPHTIPG"
BASE COUNT          799 a         1283 c         1258 g          790 t
ORIGIN      
        1 tcgtgccgtg ccggtattga aaggaaggcg cgggtatcgt cattgaagcc cacggtgttg
       61 tagaagccgg ccgtgtcagt ggtgagttcc cggaagcggc gcatgcccgc gggagaatcg
      121 tggaaccacc acgcagggcc gagtttcaat gacgggtaca cgccggcgag cggcgcaagt
      181 tcccggctgt aagtgctctc gtccagggtg aacaggatga tggagagccg ctcgtccaga
      241 ccgacggcgt cgaggagcgg cttcaagccg tcgacgtaat tggtccggac aggaatgtcg
      301 aagcccttgt cccgtccata gtcccggtga atgagccggt tgtggttgcg aaaggagccg
      361 ggatgtatct gcatcaccag gccgtcatcg atgctcatgc gcgccatttc ggtcagcatc
      421 tgggcgcgga acagttctgc gtccgccttt tcgtggcgcc cggtcctcac cttgtcgaaa
      481 agctttctcg cactgtcctg gtcgagatcc gcggtctgcg cggtggcatg accatgatcg
      541 gtggaagtag cgcccatgct tttgaaaaac tcgcgccgca cgcgttgcgc ggagaggtag
      601 ccgcgccagc tgccggtatc ttcgcctgcg agttcaccaa gccggcggag gttgtcagcg
      661 aacccgtcga actccgggtc tgtcgtcggg tcgggccggt aggccgtgat cacgcggcct
      721 tgccagccgc tgtcgcgaat cgctgcgtga tgacgcagtt cgtccagcgg cgactcggtg
      781 gtcgccagca cctcgatgtt gaagcgttcg aacagtgagc gcggacggaa ctcctcttgc
      841 gcgagcgctt cgcctatagc atcgaagtac ttatccgccg tgccggcgtc cagttgcacc
      901 ttcatgccga agacattgac gaacacccag tccagccaca gccgtgaggg cgtgccgcga
      961 aacaggtagt aatgctcggc aaacgtgcgc cagcaggcgc gcggatccgc tgctgccgtg
     1021 ccaccgccca cacgggacac accgacagca tcgagagaaa tcccatggct gaacaacatg
     1081 cgaaaaaggt agtgatccgg cgtcaggaga agttctgcgg cgttgctgaa ggagttattg
     1141 ccggcgaacc aggaggggtc ggtgtgcccg tgcgggctga tgattggctt gtctgcgacc
     1201 agcgcgtaga ggcgccgcgc aatgtcgcgc gctcgaggtt cctgcggaaa caggcgatcg
     1261 ggatgcagtt cgagcggctg agtcatccgg tcggcagcgc aaaaatatca cagtacagaa
     1321 ccaacaccag gcgtgtcacg gttgcggtca tgcaggcatc tcctttgttt gtgcgatgaa
     1381 atgatgtcag tcggaaacca gttaccgctg cacgcgtccg gaagcgtctc cgcccatcag
     1441 gttccggact tcggcaaccg acacccggtt gaaatcaccc gagatggaat gcttgaggca
     1501 acttgcagcg acggcgaact cgagcgcgtc ctgccggctg tcatacgtgt tcaggccgaa
     1561 gatcagtgcg gaggcgaagc tgtccccgcc tcccacccga tcgacgatat cggtgatctc
     1621 gtagcgcctc gagaggtgga agccgctctt gtcgcgcagg cacgccgacc agccgttgcg
     1681 gtcggcgctc atcgactcgc gtaatgtaat cgccatcacg gaaaggttcg ggtacgtttc
     1741 cagcatcttt tcgctcagtg cttcgtattt cgcgtggccg agtttgccgc tgctgacgtc
     1801 aacgtcggct gtgaccccta acgatttctg gcaatcctct tcgttcgcga taccgacgtc
     1861 gacagacttc acgagctccg acatcacctc gatagcgctc tttccgtact tccagagttt
     1921 gccgcggtag ttgaagtcgc aggacaccgt aactccctga gcctttgccg atttgacgca
     1981 tacaaaactc aagtccgccg cagactggct aagtgcgggg gtaatcccgg tgatatgcag
     2041 ccatttcgcg ccggcaagca tgcgacccca gtcgaagtca cccggcttcg cctccgcgat
     2101 ggcggagccc gcgcgatcgt agatgacctt cgatggacgc tggttggcgc cggtttcaag
     2161 gaagtagatg cccaacctgt cgccgctgcg aatgatcgaa ctcgtgtcca cgccaaaact
     2221 ccgcagctca cgtacggcgg catctccgat atcgttcttc ggcaaggcag tgacaaagcc
     2281 cgcgtcgagc ccgaagttcg ccagcgcaac cgcgacattg gcttcgccgc cgccgaacgt
     2341 cgcctcgaaa gagggcgact ggaagaagcg ctcgttgccg ggcgtcttca gccgcagcat
     2401 gatttccccg aacgacacga actgggccat gttttccttc tcccttcttt agtaagtgtt
     2461 ccggatgccg tacttgcgga gtgcctgcag cgtttttgtc aggagcgcga ccttgcgatc
     2521 gcgacggcct ccgttgccag cgatcttatc ctgccgtaat cgcggttcct gatcgcatcc
     2581 gtgggcgtaa gccagctgcc gccacaggcg atgactgagg gtactgccag gtaatcggcg
     2641 agattctccg ccgagacgcc gccggtcggc atgaacttca tttcgcgaaa tgcggaactc
     2701 aatgccctga tcatggcgac gcccccgcac acgccggcag gaaagaactt gacgacacgc
     2761 aggccgagat tgaacgcatg ctggagctca gatgccgtag caatgcccgg aaatacaggc
     2821 aggccgtgcg cattcgctac ctcgaccacc ccgtcatcga gccccggcga aacgataaat
     2881 gccgcgcccg ccgcaaccgc agcctcagct tgcgccttcg tgagcaccgt tcccgcacct
     2941 acggttgctt ccggcaagct ctcgctcaca gccttcaggc agttcagggc atcgtccgtc
     3001 cgcagcacga cctcgatgat ggagaggccg cctgcgagca gcgcttcact tgtccgcagg
     3061 gccgcctccg ggtcgtcagc ctggacgagc ggcaccacgc cggtagtcgc aagccgctct
     3121 tcgatcaatg cagtcctggc catgctgttg tcaccatgag ttcattccag gtaaccggtt
     3181 tcccgataag atatgacaac cggcttgcca gttaaaatat gccggctttt ttgccagcgg
     3241 ccatgaggtt cgttaggctg ggtggcgatg gatacactcg ccaagcagga cccgcaactg
     3301 aaggtaaacc tggagcggct caagagcacg ctgctcgaat tatcgcgcat cggcttcaac
     3361 gaagccgaca agggcatcta ccgcgtcggc ttctccgagg ccgatatggc cgcacgccgc
     3421 tggcttaagc acctgctgga gtcggaagac ctttgcccgc gcatcgacgg tgccggaaac
     3481 gtctatgcgc gccttggcga tcccgatcgc ccgacggtca ccctcggttc gcatctcgac
     3541 accgttcctg ccggcggcat gttcgatggc gcactcgggg ttgttgccgc cctcgaggtg
     3601 ctgcgcacct gcagggagca acaggtagat ctcgtctggc cgctggaatt actggcgacc
     3661 agcgaggaag aaggccgcta tggcgggatg ctcggtgccc aggccatcgc tggcgacctg
     3721 acgcccggca tcattctcaa catgcatgat gccgatggaa acatgttgtc cgacgccatg
     3781 accgcagccg gaatggatcc cattggcgca ctggacgcgc ggcggctgcc cgagaccatg
     3841 cgggcgttcc tcgagttgca catcgaacag gggccggtgc tagagaagct cgacaagacc
     3901 atcggcgtgg tcgatggaat ctccggcgtc ttcaagtgga tcgtgcgcct gataggcaag
     3961 gccgaccacg cgggcactgc cccgatgaac atgcgcagcg acgctttcat gggactcgcc
     4021 gatttcgccc atgagatacc gcgaatcatc gacgagaacg gcaccgaccg cagccgcctt
     4081 acggtcggca aggtagagct gaagcccggc agcccgcata ccattcccgg
//
LOCUS       DASUOM010000546         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_588497,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000546 DASUOM010000000
VERSION     DASUOM010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588497"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFY27_11375"
     CDS             complement(<1..634)
                     /locus_tag="VFY27_11375"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5766160.1"
                     /translation="MVSSNDWTPVTIRPRKHLGLLALVPFLLWSCRADDPTGVTNEPG
                     VLASGYWVPDGIAVIGEREFLFADRGGALYHYVDGGATRVRGLPPSRTSGVYGGLLDV
                     SLHPGFPDSRLVYIAYNDDSFGLAVARFELRDDRAESLSVIYESDEFSIGSRIEWEDP
                     SHFFLSFGVGGDPYPDAGPQDLGADVGKIHRLTADGQIPSDNPILPGALQP"
     gene            complement(921..1430)
                     /locus_tag="VFY27_11380"
     CDS             complement(921..1430)
                     /locus_tag="VFY27_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5766161.1"
                     /translation="MSVTVVVPDTVAGLDAVRELMRAFVAWHRERHQEDLHLIDRYFD
                     SAAFEAELLSLPGKYSPPHGQLLLAFAGDRPAGCVALRGIDEEVCEMKRMFVYPELHG
                     GGIGRVLADAIVEEARRLKYRTMVLDTSIRQREAQALYQRLGFERTEPYYELEDDVRD
                     WLVFMRRQL"
     gene            complement(1430..2176)
                     /locus_tag="VFY27_11385"
     CDS             complement(1430..2176)
                     /locus_tag="VFY27_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5766162.1"
                     /translation="MQTHRRLEKLRRQWDELAAFDAVGSVLTSFQRDDVIADQKFFLQ
                     GEHEIEFILDFCTRLGAAPPRGNALDFGCGIGRVTRALALRFDEAVGVDISSEMLRQA
                     RASHACLASVTLMQNGATLSMFQDSSFDFVYSSRVLQHIPDPDQVRIYLQEFVRVLKP
                     PGILVFQLPRSIRMRNRLQPRARLYSVFRSVGLPANLLLHGLRLTPMRMAAVGQEEVE
                     KTIQHAGGDSLHVELFEGGSTLYFVRKALA"
     gene            complement(2223..2753)
                     /locus_tag="VFY27_11390"
     CDS             complement(2223..2753)
                     /locus_tag="VFY27_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5766163.1"
                     /translation="MAKLVFGMNQSLDGYVDHMAFAPGPTLFRHFIEQAQEQAGSVYG
                     RQVYEVMRYWDEEQPEWDAAEHAFAAAWRRQPKWVVSRTLKSVGPNATLVGDDLEGAI
                     RELKSERDGEIEVAGPALAQSLTELGLIDEYRIYLHPVVLGHGKPYFAGPRPPLRLVT
                     NDRIGEDVVRLTYVPA"
     gene            complement(2854..3381)
                     /locus_tag="VFY27_11395"
     CDS             complement(2854..3381)
                     /locus_tag="VFY27_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5766164.1"
                     /translation="MSITIRFGLPTDASELAELAARTFHETFAADNRADDMALHLATA
                     YGPAQQHAELTSPDTITLVAEVDARLIAYAQLRTGEAPECVRGVSPVELWRFYVSREW
                     HGRGVAQALMRSVETEASRLGGRTLWLGVWERNDRAQAYYRKAGFVDKGSHAFMVGND
                     LQTDRIMERSLSPVT"
     gene            complement(3378..>3780)
                     /locus_tag="VFY27_11400"
     CDS             complement(3378..>3780)
                     /locus_tag="VFY27_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5766165.1"
                     /translation="DRGIAGNANQGGRRQVTIIEQEAWEDLMRGLKAALDPSTRRANL
                     MLSGIPLKETRGHILLIGKCRLEILGETRPCERMEESLTGLKAAMGPEWRGGVFASVA
                     AGGVIRVGDAVELRSWNGELGPSQRAAEPRR"
BASE COUNT          764 a         1205 c         1069 g          742 t
ORIGIN      
        1 aaggctgcaa cgcgccaggg aggattggat tatccgacgg gatctgtccg tcggccgtga
       61 ggcgatggat cttcccgacg tcggcgccga gatcctgcgg ccctgcatcc gggtagggat
      121 cgccacccac accgaaggac agaaagaagt gcgacgggtc ctcccactcg attctcgagc
      181 cgatcgagaa ctcgtcggat tcatagatca cgctcaggct ctcggctcgg tcgtcacgga
      241 gctcgaagcg cgcgaccgca agcccgaatg aatcgtcgtt gtaggcgatg tacacgagcc
      301 ggctgtccgg gaaaccggga tgcaggctga cgtccagtag cccgccgtaa acgccactgg
      361 tcctggacgg ggggaggccc cgaactctgg tcgcgccacc gtccacgtag tgatagagcg
      421 cacctcctcg atcggcgaag aggaactcgc gctcgccgat caccgcaatt ccatcgggga
      481 cccagtagcc ggacgcgagc acccccggct cgttcgttac gccagttggg tcgtctgccc
      541 ggcacgacca gagcaggaag ggcacgaggg ccagaagccc caggtgtttg cgcggcctga
      601 tcgtgaccgg cgtccaatcg ttgctcgata ccattccggg ctccggggcc gcgtatcgtt
      661 gcgaagtgct cggcaatgca ctctcgctcg ttgatgccgc atatcgtgat gcgttttcga
      721 agtgggatac ctgcctgctc ctcactcgct caattttcac caccgggaac cgaacgccga
      781 cgtgccggcg taagcacctg agcgtagcac ggtgcgaggg agacggcatg tccatcttgc
      841 ccggctaacc aatccttctc ggccagcctt cgtacccaca tggacgcttg acaatcgatc
      901 cggttcgata gcccgccaag ctaaagctgc cgcctcatga atacgagcca atcccgaacg
      961 tcatcctcca actcgtagta cggctccgtt cgttcgaagc ccagccgttg gtaaagcgcc
     1021 tgcgcctcgc gttgccgaat gcttgtgtcg agcaccatgg tgcgatactt cagccgcctg
     1081 gcctcctcga cgatcgcgtc ggcgagaacc ctgccaatgc cgccaccgtg caattccgga
     1141 tacacaaaca tgcgcttcat ttcacagact tcttcatcta ttccgcgcag tgcgacgcag
     1201 ccggcaggcc tgtcgcctgc gaaggccaga agaagctggc cgtggggagg actgtacttg
     1261 cctggcagcg acaacagctc cgcttcaaat gcggccgaat cgaagtaccg atctatcagg
     1321 tgcaggtctt cctggtgtct ctcccggtgc caggcaacga aagctctcat caattctctg
     1381 actgcgtcaa gaccggcgac ggtgtcggga accacgaccg ttactgacat caggccagag
     1441 cctttctgac gaagtacagt gtgctgcctc cttcgaatag ctctacgtga agggaatcac
     1501 ctccagcgtg ctggattgtc ttctcaacct cctcctgtcc aactgcggcc atccgcatcg
     1561 gagtcagcct gaggccatgg agaagaaggt tggccggcag ccctacggat ctaaagactg
     1621 agtacaatcg agcacgcggt tgcaggcgat tcctcatgcg gatgcttctc gggagttgga
     1681 agaccagtat gcctggaggc ttcagcaccc gtacgaactc ctgtaaatag attctgacct
     1741 gatcgggatc ggggatatgc tgcaggacac gggaggaata cacgaaatcg aacgaggaat
     1801 cctgaaacat gctgagagtg gcgccattct gcatgagcgt aacgctcgcg aggcaagcat
     1861 gactggcacg agcctgcctc agcatctcag acgagatatc tacgccaacg gcttcatcaa
     1921 aacgaagggc taacgcacgg gtgacccgtc cgatcccaca accaaagtcg agggcattgc
     1981 cgcgcggtgg agccgctcct aaacgtgtgc agaaatcgag aatgaactcg atctcgtgct
     2041 ctccctgaag gaagaatttc tgatcggcga tcacgtcatc acgttgaaag ctcgtgagta
     2101 cgcttccgac cgcatcaaaa gctgccagtt catcccattg gcgacgcagc ttctctaatc
     2161 tccgatgcgt ttgcataacc tcgtcggctc ctgcctgtcg cagcggattc cgagtcgcga
     2221 catcaggcag gaacgtaggt caacctgacc acatcctcgc caatccgatc attggtcaca
     2281 agacggagcg gcggccgggg gccggcgaaa tatggcttgc cgtgaccaag tacgacgggg
     2341 tgcaggtaga ttcgatactc gtcgatcagg ccaagttcgg tgaggctttg cgccagggcc
     2401 gggccggcaa cttcgatctc gccgtcgcgc tcggacttca gctcacggat cgcgccctcg
     2461 agatcatccc caacaagcgt ggcgttgggg ccaaccgact tcaacgtgcg cgagacgacc
     2521 catttcggct gcctccgcca cgccgctgcg aaggcgtgtt ccgctgcatc ccactcaggc
     2581 tgttcctcat cccagtaacg catgacctcg taaacctggc gaccgtacac actacctgcc
     2641 tgctcctgag cctgctcgat gaagtggcgg aagagcgtgg ggcctggcgc aaacgccatg
     2701 tggtcgacgt agccgtccag ggactggttc attccgaaca cgagcttagc catacccact
     2761 tccctcccgt gctgctccgc gcggttctat cgtgttatgc aatcggttgc aagcgacatc
     2821 gactgatgca tatcgcaatg agttgtggcc atttcaggtg acaggcgaga gcgatcgttc
     2881 cattatgcga tcggtttgca ggtcgttccc gaccatgaac gcgtgcgagc ctttgtccac
     2941 gaagccggct ttacgataat aggcctgcgc ccggtcgtta cgttcccata ctcccagcca
     3001 gagggtgcgg ccgccgaggc ggctcgcttc ggtctcaacg ctcctcatga gcgcctgagc
     3061 aacgccgcgc ccgtgccatt cccgggagac atagaagcgc cagagttcga cgggcgacac
     3121 accacgtacg cactccgggg cctcaccagt tcgcaactgg gcataggcga tcagtcgggc
     3181 gtcgacctca gccaccaggg tgatggtgtc cgggctggta agttcagcgt gctgttgcgc
     3241 cggcccatag gcagtggcga gatgaagcgc catgtcatcg gcgcgattgt ccgcggcaaa
     3301 ggtctcatga aacgttcgag cggccaactc ggcaagttct gacgcatccg tcggaaggcc
     3361 gaatcgaatt gtgatgctca tcgccgtggc tcggccgcgc gttggctcgg gcccagttca
     3421 ccgttccaac tccgcaactc tacagcatct ccaactcgaa tgacgccgcc cgctgcaaca
     3481 ctcgcgaaca cgccacctcg ccactccggt cccatggctg ccttcaatcc ggtgagtgat
     3541 tcctccatgc gttcacaggg cctggtctcc cccagtattt ccaggcggca cttaccgatc
     3601 aatagaatat gcccacgcgt ctccttcaac ggtattccgc tgagcatcag attcgctcgc
     3661 ctggtggacg gatccagagc tgctttcaga cccctcatca gatcctccca tgcctcctgc
     3721 tcgatgatgg ttacctgacg ccgtccgccc tgattcgcgt taccagctat cccacgatcg
//
LOCUS       DASUOM010000547         4580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_90608,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000547 DASUOM010000000
VERSION     DASUOM010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90608"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1634)
                     /gene="ilvA"
                     /locus_tag="VFY27_11405"
     CDS             complement(93..1634)
                     /gene="ilvA"
                     /locus_tag="VFY27_11405"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008896918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX5766166.1"
                     /translation="MSTSPNLQNAVIDDYLERIENARVYEVATETPLDVARNVSRRLK
                     NRILLKREDLQSVFSFKLRGAYNKLVKLPRDVLDKGVICSSAGNHAQGVALAAARLNV
                     RAVIVMPVTTPAIKVDAVRSLGGEVVMHGDTYDDAEQHARQLRDEQQLTFIHPFDDPD
                     VIAGQGTIGREILKQSSTPIHAVFVPIGGGGLISGIAAYIKETNPAVRVIGVEPDDAA
                     GMQQSLAAGRPITLDHVGIFADGVAVRRVGDETFRLCQHYVDEVITVDTDQTCAAIRD
                     IFEETRSIAEPAGALSLAGIKKYLAREKLRDATLVAVIGGANVNFDRLRHIAERAAVG
                     EQREMLLAVEIPEQPGSFRKFCEAIGRRSVTEFNYRYSDSAKAHIFAGIELRHGQAER
                     TRLIKKLSSLGYQIQDLSDNELAKVHVRHMVGGGAMPENEKLFRFEFPERPGALLDFL
                     NAIGSSWNISLFHYRNHGSDHGRILAGIQVPPAEVNELEAHLAVLGYAHWDESGNPAY
                     RMFLA"
     gene            1841..3160
                     /locus_tag="VFY27_11410"
     CDS             1841..3160
                     /locus_tag="VFY27_11410"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5766167.1"
                     /translation="MQTSEPVRLSLARPACRPKDGTPLFRAEAMQSAREPLHGPIAVV
                     MPPSALAGASLSLVTLLMLALAAFIVEVPQRTAAVGVLMPPDGFTKIVAETSGQVIDV
                     NVAAGDLVEAGQALLSVSSDRSVIGHGTVSSNQLRSLRNERRLQGEANLRRQRVQSER
                     LRTVDGQLDSLELRLELLGREMKIQDSRSTLLGARFDRLHELAASGNVPAVQLDDQRL
                     ALLDEQAGAAALQQRAAQLEEERAELAQARVALVEEAATRQIEFAIALEQLQRQIASL
                     EASVSSELQAPASGVVALVTVRAGQVVRPGQTLVTLQRGKSDLEAWLYLSSANAGLLR
                     AGQEVQLRLDSYPHQMFGTLPATIASISHVALLPSDLDVPLAITGPVFELRATLDAQH
                     ITALQRDWPLAAGTSVHADVVQRRYRLYEWLLRLRQTDSATDSSADA"
     gene            3153..>4580
                     /locus_tag="VFY27_11415"
     CDS             3153..>4580
                     /locus_tag="VFY27_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX5766168.1"
                     /translation="MHERLLGLKRGRRVPLILQREHAECGLACVAMVAGWFGHHVPLD
                     VLRSLTPPSSRGATFNDLLRTATELNLSGRPLRLQFSDLRRLALPAILHWRMQHFVVL
                     TRLGTRNATIHDPACGKRRVCMKELHAGFTGVAMELHQAKGFVRRAASGSLSFLSFLA
                     SCRNLGRYLAVMLLLLVAIQVLALVPPVATQLLIDEVVLGQDVEWLYRALGGLALVLL
                     IALLLDVMRRWIALYAGSVLAADSTFNVVRHLYNLPAQFLKNRHLGDLMSRLESLVPV
                     RKAITETALEGIVQAAVLLATLGIMLFYSPWLTLVSAAGLAVSGILIATILPAMRRLG
                     EQTLVHKALGDSSLLETMRACDSTLALGLAPLRLAQWQNHFVAAMNTRVRQSKLSIWH
                     GFGTGLVGGTEQVLFLGVGITQLIDKQLTLGVLFAFMTLRNRLGAAAISLFGVLQEMF
                     MLKVHVDRLSDIVLAEPLPPVQPGAL"
BASE COUNT          783 a         1475 c         1444 g          878 t
ORIGIN      
        1 atgccggcgt gccgcaacat accgctgcag gagcctgccc ggcgggcgac gtctcctctg
       61 caaaccggcc cttgttgccg tgccggtaat gtctacgcca ggaacatcct gtaagcggga
      121 ttcccgctct catcccagtg agcgtacccg agcaccgcga ggtgcgcctc gagttcattg
      181 acttctgcgg gcggcacctg tatgccggcg agtatgcggc cgtggtcgga gccgtgattg
      241 cggtaatgga acaggctgat gttccagctg ctgccgatcg cgttcaggaa atccagcaac
      301 gcgcccggcc gctccggaaa ctcgaaacgg aacagctttt cgttttctgg catggcgccg
      361 ccgccgacca tgtggcgcac gtgcaccttc gcgagttcgt tgtcgctcag gtcctggatc
      421 tggtaaccga gggagctgag ctttttgatg agccgtgtcc gttccgcctg cccgtgccgc
      481 agctctatac ctgcgaagat gtgcgctttg gccgaatcgc tgtagcgata gttgaactcg
      541 gtgacgctgc ggcgaccgat cgcttcgcag aactttcgaa aacttcccgg ctgctccggg
      601 atctctaccg ccaggagcat ctcccgctgc tcgcccacgg cggcgcgttc ggcaatgtgg
      661 cgcaggcggt cgaaattgac gtttgcgccg ccgatgacgg caaccagtgt cgcgtcacgc
      721 agcttctcac gagccaaata cttcttgatg cccgcgagtg aaagcgcgcc ggcgggttcg
      781 gcaatcgacc tggtctcttc gaagatgtcg cggatggccg cacaggtctg gtcggtgtcg
      841 acggtgatga cttcatcgac gtagtgttgg cacagccgga atgtctcgtc ccccacccgc
      901 cgcacggcca cgccatcggc aaagatgccg acgtggtcca gcgtgatggg ccttccggcg
      961 gcgagcgact gctgcatgcc ggcggcatcg tcgggctcga cgccgatcac ccgtacggcc
     1021 ggattcgtct cctttatata ggcggcaata ccggagatca gcccgccgcc gcctatcggc
     1081 acgaataccg cgtggatcgg cgtgctgctc tgcttgagga tttcccggcc gatggtgccc
     1141 tggcctgcga tcacatcggg atcgtcgaac gggtgaatga aggtgagctg ctgctcatcc
     1201 cgcaactgcc gcgcgtgctg ttctgcatcg tcatacgtat cgccatgcat gacgacttca
     1261 ccgcccagcg agcgtacggc gtcgactttg atggcgggag tcgttaccgg catcacgatg
     1321 accgcgcgga cattaaggcg cgccgccgcg agcgccactc cctgcgcgtg gttaccggct
     1381 gaactgcaga tcacaccctt gtccagtacg tcccgcggca gcttcacgag tttgttgtag
     1441 gcgccccgga gcttgaagga aaacaccgac tgaaggtcct cccgtttcag caggatgcga
     1501 tttttcaacc tcctggaaac gttgcgagcc acgtccagcg gagtttcagt tgcaacctca
     1561 tagacgcggg cgttttcgat cctttcgagg taatcgtcga taacagcgtt ttgtaagttc
     1621 ggactcgtgc tcatacaggc tccggcgtcc ttcgcggatc tcagcgcaga ggacaaagtg
     1681 ccgggaagag tagcgcattt gtcatggcgc tgacgaacca gcacgaggga aagtcgaatc
     1741 ggtgcaaaga gcgctgaaag gctcatcgcc cgtcgacgcc agccatgttt gcattgcgct
     1801 tgagtaaatc agcgcaagct gaacagccaa agaagtggag atgcaaacga gcgaacctgt
     1861 ccgcctcagc ctcgcaaggc cggcgtgccg cccgaaggac gggacgccgc tgtttcgtgc
     1921 cgaagcgatg cagtcggcac gggaaccgct gcacgggccg atagcggtgg tcatgccgcc
     1981 ttctgccctg gcaggagcga gcctgagtct ggtgacactg ttgatgcttg cgctggcggc
     2041 attcatcgtt gaagtaccgc agcgcacggc ggcggtaggc gtgctgatgc cgcccgacgg
     2101 tttcacgaaa atcgtcgcgg aaacctctgg ccaggtcatc gatgtgaacg tcgctgcagg
     2161 tgatctggtc gaggcgggac aggcactgct cagcgtcagc tccgaccggt cggtgatagg
     2221 ccacggtacc gtttcctcga accagctccg gtccctgcga aacgagcgtc gtttgcaggg
     2281 tgaggctaac ctccggcgac agcgggtgca gtccgaacga ctgcgcactg tcgacggcca
     2341 gctggacagc ctcgaactgc ggctagagct tcttggccgc gaaatgaaaa tacaggactc
     2401 gcgcagcaca ttactcggcg cgcgcttcga ccgcctgcat gaactggccg cgagcggcaa
     2461 tgttcccgcc gtccagctgg acgaccagag gctcgcgctg ctggacgagc aggcaggtgc
     2521 cgcggcgctg caacagcggg cggcgcaact ggaggaggaa cgcgccgagc ttgcacaagc
     2581 gcgggttgcc ctggtagagg aagcggcaac ccggcagatt gagttcgcaa ttgctctcga
     2641 gcaattgcaa cggcagatcg cctcgctgga ggcctccgtg agcagcgaac tccaggcacc
     2701 cgcgagcgga gtcgtggcac tcgtcacggt tcgcgccggc caggtggtgc gccccggcca
     2761 gacgctggtt acgctgcagc gcgggaaatc ggatctcgag gcatggcttt acctgtccag
     2821 cgcaaacgcg gggctgttgc gggccgggca ggaggtgcag ttgcgcctcg attcctatcc
     2881 gcaccagatg ttcggcacgt tgccggccac cattgcatcg atatcgcatg tagccctgct
     2941 gccgtccgat ctggatgtac ccctggccat caccgggccc gtattcgagc tccgggcgac
     3001 gctggacgcg cagcacatca ccgcactgca gcgcgactgg ccgctggctg ccggaacctc
     3061 cgttcatgcg gatgtcgtgc agcgacgcta ccggctgtac gaatggctgc tgcgactgcg
     3121 ccagaccgac agcgcgaccg actcttcagc cgatgcatga gcgcctgctc ggactgaagc
     3181 ggggccgccg cgtcccgctg atcctgcagc gcgaacacgc ggagtgcggc ctcgcatgcg
     3241 tggcgatggt cgccggctgg ttcgggcatc acgtacctct ggacgtgctc cgctcgctaa
     3301 cacccccatc gtcccgcggc gcgaccttta acgacctgct gagaacggcg acggagctga
     3361 acctgtccgg ccgtccgctg cggctccaat tttccgacct gcgccgcctc gcgttgcccg
     3421 cgatcctgca ctggcgcatg cagcatttcg tggtgctgac acgcctcggc acccgtaatg
     3481 ccaccattca cgatccggcg tgcggcaagc gcagggtctg catgaaggag ttgcacgcgg
     3541 gcttcacggg tgtcgcgatg gagcttcatc aggcgaaagg attcgtgcgt cgagccgcgt
     3601 ccggttccct gagcttctta agctttctcg ccagttgccg caatctcggg cgctacctcg
     3661 ccgtgatgtt gttgctgctg gttgcgatcc aggtgttggc gctggtgccc ccggtggcga
     3721 cgcagctgct gattgacgag gtggtgctgg ggcaggacgt cgagtggctg taccgcgccc
     3781 tcggcggcct ggcgctggta ctgctgatcg ccctgttgct ggacgtcatg cgccgatgga
     3841 tcgccctgta cgccggcagc gtgcttgcag ccgacagcac gttcaacgtc gtccggcacc
     3901 tgtacaacct gccggcgcag ttcctcaaga accgccacct cggcgacctc atgtccaggc
     3961 tcgaatcgct ggtaccggtc cggaaggcca tcaccgagac tgccctggag ggcatcgtgc
     4021 aggcggcagt cctcctggcg acgctcggca tcatgctgtt ctacagcccc tggcttacgc
     4081 tggtgtcggc agctggcctc gcagtctcgg gcattctcat cgcaacaatc ctgcccgcga
     4141 tgcgccgcct gggcgaacag accctggtcc acaaggcgct cggcgacagc tcgttgctcg
     4201 aaacaatgcg cgcctgcgat tccacgctcg cactcggcct cgccccattg cgcctcgcgc
     4261 agtggcagaa ccatttcgtc gccgcgatga acacgcgcgt gaggcaatcg aagctttcca
     4321 tctggcacgg cttcggcacg gggctggttg gcggcacgga acaggttctc ttcctcggcg
     4381 tcggcatcac ccagctcatc gacaagcagc tgacgctcgg agtgctgttc gctttcatga
     4441 cgctgcgcaa tcgcctcggc gccgccgcga tatccctgtt cggcgtgctg caggagatgt
     4501 tcatgctgaa agtgcacgtc gacaggttgt ccgacatcgt cttggcagaa cctcttcccc
     4561 cggttcaacc cggcgccctc
//
LOCUS       DASUOM010000548         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_623736,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000548 DASUOM010000000
VERSION     DASUOM010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623736"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1726)
                     /locus_tag="VFY27_11420"
     CDS             complement(290..1726)
                     /locus_tag="VFY27_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5766169.1"
                     /translation="MLDRRAFLRRAASLGAGSMFAPSLASLSNWSRIDETIARTLRPN
                     DNAGLGPLVTSVDCPEFQIPDSFRCIRISRSLGASAANPNFIVPNALDGMATFSLPNG
                     DIRLIRNHEMNQPAARAVPLGNKPYDPKASGGTTSLTVRLEGTGADRSVRVLDEYVSI
                     GGTHVNCAGGRTPWESWLTCEETTAGPSSGFERPHGYIFEVPVAAKEAVDPVALTAMG
                     RFVHEAVAVDPRDNYVYETEDMRWNAANEQSLPGAGFYRFVPRTPQRLAEGGQLQILA
                     VRDRPRHNTVIGQTVGARLPAHWINIEAPDPADAERDPSAVFREGLSKGAAIFDRLEG
                     CFWADDSCYFVATSGGTARAGQVWRYVPESNDSGYITLVFESPSRSVLDAPDNICVSP
                     RGGIVICEDGSAEQYVRALSTSGQLVNLALAPLVTGEPSPREFAGCCFSPDGEVLFFN
                     VQGSTTSYGTRPGTTYALWGPWTQAVGV"
     gene            complement(1811..>2262)
                     /locus_tag="VFY27_11425"
     CDS             complement(1811..>2262)
                     /locus_tag="VFY27_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5766170.1"
                     /translation="HANVSSIDLSLVAQTIAGIQDKAAAAVRFTAIFSLMMGIPVLMS
                     AVAATRRERVREGVLLRALGASRRQIQRIMLAEYATLGALGTVTGVLLGIAGAWAAMW
                     FLFEQRTLVIAPEVAVVGGIMIALTVTIGYLSGRDVFRAPPAEALRE"
BASE COUNT          456 a          713 c          673 g          420 t
ORIGIN      
        1 atggcctgat ccacagcttg cgcgacggtt cgcgggaaag ttgcggcgga gtcagcggtc
       61 tgctgcgcag cggtcgcgtg cgatgactgc acggggcgac gcgtagcgca tgcgagcagc
      121 gtaacggcca ggaaaaagcc gagggggacc tggcggaggg acatacgtga ataatagcga
      181 gcgcgttgga ttgaacgctg ctttttacga tcagccggcc tgcgctatcg cagcatctca
      241 ggatcctccg cgaggcgggg cttcgtatca gtgcgcgact agggacgaat cacacgccaa
      301 cagcctgcgt ccacggaccc cacaacgcgt acgtagtgcc gggtcgggtg ccataactcg
      361 tggtcgagcc ctgcacattg aagaagagca cttcgccgtc gggactgaaa cagcaccccg
      421 cgaattcgcg gggcgatggt tctccggtta ccaatggcgc aagcgcgaga ttcaccaact
      481 gccccgatgt cgacagcgcg cgcacgtact gttcggccga tccatcctcg caaatgacaa
      541 tcccgccgcg cgggctcacg caaatattat ccggagcatc gagcaccgac cttgacggcg
      601 attcgaaaac aagtgtgata tatcccgagt cgttactctc cggcacataa cgccagacct
      661 gtcccgcgcg cgcagtgcca cccgaggttg caacgaagta acagctgtca tcggcccaga
      721 aacatccttc gagccggtcg aaaatcgccg cgcctttcga caatccctcc ctgaagacgg
      781 cagatggatc acgctccgca tcagcaggat ccggcgcctc gatgttgatc cagtgtgcgg
      841 gcagtcgcgc cccaacagtc tgtccgatta ccgtgttgtg ccgggggcga tcccgcacag
      901 cgagtatttg caattggccg ccttcggcaa gtctctgcgg cgtcctcgga acgaaacgat
      961 aaaagccagc gcctggaagt gactgctcat tggccgcgtt ccagcgcata tcctccgttt
     1021 cgtagacata gttgtctcgg ggatcgaccg cgaccgcctc gtgcacgaag cgtcccattg
     1081 ctgtgagcgc aactgggtca accgcttctt tcgccgcgac tggaacttcg aagatgtaac
     1141 cgtgcggcct ctcgaatcct gatgacggac cggcggtcgt ttcctcgcag gtgagccagc
     1201 tttcccaggg cgtgcggcca ccggcgcaat tcacgtgcgt tccaccgatc gacacgtact
     1261 cgtccagcac gcgaacactt cgatctgcac ccgttccttc gaggcgcacc gtgagtgaag
     1321 tggtgccgcc actcgctttc ggatcgtacg gtttgttgcc gagcggcact gcgcgtgccg
     1381 cgggctgatt catttcgtga tttctgatga gccttatatc gccattcggc agcgaaaacg
     1441 tggccatgcc atccagggca ttgggaacga tgaaattcgg attggcggcc gacgccccga
     1501 gcgagcgact tatgcggata caccggaaac tgtcggggat ctggaattcg ggacaatcga
     1561 cgctcgtgac cagtgggccg aggccggcgt tgtcgttcgg ccgcagagtg cgagcgatgg
     1621 tctcgtcgat tcggctccag ttcgagagcg aagcaagcga aggtgcgaac atcgagccgg
     1681 cgccaagtga tgcggcccgt ctcaggaaag ctcgtcgatc aagcatactc gttggtctcc
     1741 ggtgacagac tgaatcgggg acagtctact ctgcccccat gaaccgctgt ctctgtcccc
     1801 atgaaccaat ttactcgcgc aacgcctcgg cgggaggagc gcggaataca tcgcggccgg
     1861 acaggtaccc gattgtgacc gtcagcgcga tcataattcc accgacaacc gcgacttcgg
     1921 gtgcaatcac cagtgtgcgc tgctcaaaca ggaaccacat cgcggcccac gctcctgcga
     1981 tgccgagtag aacgccggtc acggttccga gcgcgcccag cgtcgcatac tcggccagca
     2041 ttatccgctg gatctggcgg cgactcgccc ccagagcgcg gagcagcacc ccctctcgca
     2101 cacgttcacg acgcgtcgcg gcaactgcac tcatcaacac ggggatcccc atcatgaggc
     2161 tgaaaattgc cgtgaatcgc acggccgcgg cggccttgtc ctggattccc gcgattgtct
     2221 gcgccacgag actcaggtcg atcgatgaaa cgttcgcatg cc
//
LOCUS       DASUOM010000549         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1151958,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000549 DASUOM010000000
VERSION     DASUOM010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151958"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFY27_11430"
     CDS             complement(<1..312)
                     /locus_tag="VFY27_11430"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138585.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5766171.1"
                     /translation="MRFTDFGLVTLKEIPAEAEIVSHQLMLRAGMIRRLAAGLFTWMP
                     LGVRVLRKVERIVREEMDRAGALEVLMPAVQPAELWKESGRWDKYGSLLLRMKDRHDR
                     EY"
     gene            451..1077
                     /locus_tag="VFY27_11435"
     CDS             451..1077
                     /locus_tag="VFY27_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766172.1"
                     /translation="MNGQRAFFVAAEGIPFLLAAALAIAAAWYICGLAWALLPFALFI
                     WLYLVFRDPWREVASAPLGVVSPVDGTVAELSSDNYGDDESKEAGVQRIVIRVSSLGT
                     YTARSPVEGKITDFGVTPEPPDGAPGLWLLTDEGENVVLRFRGNRFGIAPRASLTYGE
                     RVGQGQRCAYLRLTKFAEIELPPGSRLLVTEGQRVSAATDLLAMLPQA"
     gene            1346..2140
                     /gene="pssA"
                     /locus_tag="VFY27_11440"
     CDS             1346..2140
                     /gene="pssA"
                     /locus_tag="VFY27_11440"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX5766173.1"
                     /translation="MEPEPESVRRRGIYLLPNLLTTAALFAGFYSIVAAVDGSFERAG
                     AAIFVAMILDSLDGRVARLTKTSSQFGKEYDSLSDMVSFGLAPAIVVYQWGVARLAEW
                     NVTWGRVGWLVAFLFAVAAALRLARFNSQIATADKRWFVGLPSPSAAGVVAAFVWLSS
                     KLDITGLGGLAGAFLVTAAAGALMVSDFRYYSFKEIHFGRRIPFTWMLAIPLIFMIIS
                     IDPPLVFLLMFGIYALSGPAFWYWLRRRPAVDNVTNDPAEEAEDDR"
     gene            2130..2831
                     /locus_tag="VFY27_11445"
     CDS             2130..2831
                     /locus_tag="VFY27_11445"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5766174.1"
                     /translation="MTAESTRLAYLDAIGIDVFAPRATERATAEPVLDHEPVERSAVD
                     ADPFGWPELRRAVAACTRCALHKGRTQTVFGVGNPDADWMIVGEAPGAEEDRRGEPFV
                     GRAGQLLDEMLRAVGLSRESAFIANILKCRPPNNRDPAASEAEACRSYLERQIEFVHP
                     RIILAVGRIAAQQLLRSEESVGRLRGRVHTLANPRIPVVVTYHPAYLLRTPTQKREAW
                     KDLCLARRIGGEHTE"
     gene            2828..3295
                     /gene="rimI"
                     /locus_tag="VFY27_11450"
     CDS             2828..3295
                     /gene="rimI"
                     /locus_tag="VFY27_11450"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5766175.1"
                     /translation="MISPIPQVPVHTRTMVHDDLAQVSDIERRSYDFPWSHGVFRDCL
                     LAGYTCIVLERGDTVAGYGILSVAAGEAHVLNICVDPDYRRLGYGDRLLDELLARARS
                     ASVKEVFLEVRPSNVNALSLYRKKGFRQIAQRRAYYQARSGREDAAVLSLVVK"
     gene            3399..>3684
                     /locus_tag="VFY27_11455"
     CDS             3399..>3684
                     /locus_tag="VFY27_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5766176.1"
                     /translation="MNSLEKHVKQRRTFAIISHPDAGKTTLTEKLLLYGGAIQLAGTV
                     KGRKATRHATSDWMALEQQRGISVTSSVMQFPYRDRLVNLLDTPGHEDFSE"
BASE COUNT          653 a         1133 c         1133 g          765 t
ORIGIN      
        1 gtattcgcga tcgtgacgat ccttcatgcg cagcagcagg gagccgtact tgtcccagcg
       61 gcccgattcc ttccataact ccgctggctg aaccgcaggc atcagtactt caagcgcgcc
      121 tgcgcgatcc atttcctcgc ggacgatccg ctccaccttt ctcaggaccc tcacgccgag
      181 cggcatccac gtaaacagtc ctgcagcgag ccgccgaatc attccggcac gcagcatcag
      241 ctggtggctt acgatttccg cttccgccgg aatctctttc agggtaacta gtccgaaatc
      301 ggtaaatctc atgtactttt ccggccttca gtatcggggg acgcgacggt tcggtagtcc
      361 gcggcgaatg gcgggcgtat tctacacgac ccgccgcacg gccggtccgg gcgccgtcca
      421 tcgatatcag acgttgggga gtaaggcctt ttgaacggac agcgagcatt tttcgtcgcc
      481 gcagaaggca ttccgttcct gctcgctgca gcgctggcaa tcgccgcagc gtggtatatc
      541 tgtggcctgg cctgggcatt gctcccgttc gccctgttca tctggttgta cctggttttt
      601 cgcgatccgt ggcgcgaggt cgcctcggcc ccgcttggcg ttgtaagtcc ggtggatggc
      661 acggtcgcag aactgtcttc ggacaactac ggggacgacg aaagcaagga agctggtgtc
      721 caaagaatcg tcatccgcgt ctcgagcctc gggacgtaca ccgcacgctc tcctgtcgag
      781 gggaagatta cggatttcgg ggtaactccg gagcctccgg acggcgctcc cgggctctgg
      841 ctcctgacgg acgaaggcga gaacgtggtc ttgcgattcc gcggcaaccg gttcggcatt
      901 gcgcctcgcg catcccttac ttacggggag cgggtgggcc agggccagcg ctgcgcctac
      961 ctgcggctga cgaaatttgc cgagatcgaa ttgccgcccg gttcgcggtt gctggtgacg
     1021 gaaggtcaac gtgtgtccgc cgcaacggac ctgctggcga tgctgccgca ggcatagtgc
     1081 cctgaatcag tagttcatcg catccttcgc ggcgtcttcg gggcacctgc acaagccagg
     1141 ttcaagacaa ctgttggagc ccgccatgcg ggcgagtgcg atggacataa gctaactcgc
     1201 ccgcatggca ggctcctaca gtgctcctcc cgacttgtgc agagcttgcc tcggagccat
     1261 tgcacccccg attgttaacc tctcttgggg catggcagta ttaacggccc tcggacggcc
     1321 ttttccgcga gaaacctgca gggtaatgga accggaaccc gaaagcgtca ggcgacgcgg
     1381 catttatctt ttgcccaacc tgcttacgac ggcagcgttg ttcgcgggct tctattccat
     1441 cgttgccgcc gtcgacggca gctttgagag ggcgggagcg gctatcttcg tagcgatgat
     1501 cctggactcg ctggatggca gggtcgcgcg cctgacaaag acttcgtcac agttcggcaa
     1561 ggaatacgac agcctgtcgg acatggtgtc gttcggactt gcgccggcga tcgtcgttta
     1621 ccagtggggc gtcgcgcggc tggctgaatg gaacgtcacc tggggcaggg taggctggct
     1681 ggtcgcgttc ctgttcgccg tcgctgccgc gctaagactg gcccgcttca actcccagat
     1741 agccacagcg gacaagcgat ggttcgtcgg tttgcccagc ccttcggcag ctggcgtcgt
     1801 ggccgcattt gtctggctga gcagcaagct cgacatcacg gggcttggcg ggctcgcggg
     1861 cgcgttcctg gtcactgccg ccgcgggcgc gctgatggtt tcggatttcc gctactacag
     1921 tttcaaggaa atccacttcg ggcgtcgcat cccctttacc tggatgctcg ccatacccct
     1981 catattcatg atcatctcga tcgacccgcc gctcgtattc ctgctcatgt tcggcattta
     2041 cgcattgtcc ggccccgcat tctggtactg gttgcggcgc aggccggccg tggataacgt
     2101 gacgaacgat ccggcggagg aagcagaaga tgaccgctga gtcgacgcgt ctcgcatacc
     2161 tggatgccat cggcatcgat gtgttcgcgc cgcgcgcaac cgaacgagcg actgccgaac
     2221 ccgtgcttga ccacgaaccc gttgagcgat ccgctgtcga cgcggatccg ttcggctggc
     2281 cggaactgcg gcgtgccgtt gcggcttgca cgcgctgtgc cctgcacaaa ggccgcaccc
     2341 agacggtgtt cggtgtgggc aacccggatg ctgactggat gatcgtcggc gaagcgcctg
     2401 gtgccgaaga agaccgccgt ggcgaaccct tcgtcggccg tgcgggtcag ttgctggatg
     2461 aaatgctgcg cgcagtcggc ctgagccgcg agagtgcctt cattgcaaac atcctgaagt
     2521 gccggccgcc gaacaatcgc gaccctgctg cttccgaggc tgaagcatgc aggagctacc
     2581 tcgagcgaca gatcgagttt gtgcatccca ggatcatcct cgccgtcggg cgtattgccg
     2641 cgcagcaatt gcttcgcagc gaggaaagtg tgggcaggtt gcgaggccgc gtgcatacac
     2701 ttgctaatcc gaggatacct gttgttgtga cttaccaccc cgcttacctg ttgcgcaccc
     2761 cgacacaaaa gcgcgaggcc tggaaagacc tttgtctcgc gcggaggatc ggcggagagc
     2821 acacggaatg atttccccga ttccacaagt gccggtccac acccggacga tggttcatga
     2881 cgacctcgcg caggtatccg acatcgagcg gcgcagttac gactttccct ggagccacgg
     2941 cgtgtttcgc gattgcctgc ttgccggcta cacctgcatc gttctcgaac gcggcgatac
     3001 agtcgcgggc tacggcatcc tttcggtagc cgccggcgaa gcacacgttc tgaacatctg
     3061 cgtcgacccg gattaccggc gactgggcta tggcgatcgc ctcctggacg agttactggc
     3121 acgggccagg tctgcatcgg tcaaggaggt attcctggag gtaaggccgt cgaacgtcaa
     3181 tgctctgtct ctttaccgca agaagggctt ccgccagata gcgcagcgtc gggcgtacta
     3241 ccaggctcgt tccgggcggg aggatgcggc ggtcctgtcg ctcgtcgtga agtaacgccg
     3301 ggcgccgttt gttcgccctg ttgccatggt cgtatggcgg gaatagcggt aaacttgcgc
     3361 gctttttagc cgcgcccgcc gccatgttgg ctctgaacat gaattccctg gaaaaacacg
     3421 tcaaacaacg tcgcacattc gcgatcattt cacatcctga tgccggcaag accacgctga
     3481 ccgaaaagct attgctctac ggcggcgcga tccagctggc gggcaccgtg aaaggccgta
     3541 aggccacccg tcatgccaca tccgactgga tggcgcttga acagcagcga ggaatctcgg
     3601 ttacgtcttc ggtgatgcag tttccgtatc gcgaccggct ggtcaacctg ctggacacgc
     3661 cggggcacga ggatttctcg gaag
//
LOCUS       DASUOM010000550         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1157704,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000550 DASUOM010000000
VERSION     DASUOM010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157704"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFY27_11460"
     CDS             complement(<1..308)
                     /locus_tag="VFY27_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766177.1"
                     /translation="MLLAIILLAGCASAPRNVPYPAFMQAEDLDGAFHAGLPGTRAKV
                     LSGDPRTGGFSALLLLPEQWRWNTGAAPGKSVEIFVLEGEVMLGDLALRPGNYAWLPP
                     G"
     gene            complement(353..>2269)
                     /locus_tag="VFY27_11465"
     CDS             complement(353..>2269)
                     /locus_tag="VFY27_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX5766178.1"
                     /translation="GQEYPPYVPAEDSIAEFTIKAAFFGILFGIVFGAANAYLGLRAG
                     LTISTSIPVAVMTVAAFRALQTVGKTGTILEANLAQTVGSASSSLASGVIFTLPALFL
                     WGLSPGLLQMTLLAMCGGLLGVLFMIPLRRFLIKGEHGRLPYPEGTACAEVLVANEVG
                     GSSARYVFYGLGLGAFFKFLTSWLHIIPEAAWTRLPFIRKGELGMDLSPALFGVGYIL
                     GPRIAAVMVGGGLLAWVLIIPGIAWWGDGFIAPVFPESTALIRDMSASELWSRYVRYI
                     GAGAVATAGIVTLIRSIPVMVQSFRIGTAQIRERVGDDGTIRAAVPRTDNDLPLKFIG
                     IGLLAIVLFLGLVPQVFGDVGGLGVRLIAALCVVVFAFFFVTVASRIVGLVGVTSNPT
                     SGMTIASLLGTAGVFLVMGWTDMTGKAAALTVGCVVAIAASIAGDTSQDLKTGYLLGA
                     TPRRQQVGELVGVLTSATFVCLSVLLLAETFGFGGTELPAPQATLMKLVIEGVLDQQL
                     PWGFVAIGIGIALACELLKIPSLPFAVGVYLPVSTMTPLFVGGLLRGLFERRARTEAE
                     ASEYRERGILLGSGFVGGEGLLGVGIAAVAFMQSRKPEGIGIEWLGTPGIAQLVGLVA
                     FGFFIAGFVRLVRR"
BASE COUNT          483 a          762 c          694 g          330 t
ORIGIN      
        1 ccgggtggca gccaggcata gttgccgggg cgcagcgcca ggtcgcccag cattacctcg
       61 ccctccagca cgaatatttc cacgcttttg cccggggccg cgccggtatt ccagcgccat
      121 tgttccggta gcaagagcag cgcgctgaat ccgccggtgc gcgggtcgcc gctcaggacc
      181 ttcgcacgag tgccgggaag gccggcatgg aacgcgccat cgaggtcttc ggcttgcatg
      241 aaggccgggt acgggacgtt gcgcggtgcg ctcgcgcatc cggcgagcag gatgatggcc
      301 aggagcatca gtgtgaaggc tgtccaactc cccctggccg gcttcattac gctcaccgcc
      361 gaaccagtcg gacaaagccc gcgataaaga aaccgaaagc aaccagcccc acgagctgag
      421 ctatgcctgg tgtaccgagc cattcgatgc ctatgccttc cggcttgcgg ctctgcatga
      481 acgctacggc ggcgatgccg actccgagaa gaccctcgcc gccgacaaag ccgctgccca
      541 gcaggatgcc acgctcgcga tattccgaag cctcggcttc cgttctcgcg cggcgctcga
      601 acaggccgcg cagcagccca ccaacgaaca gcggcgtcat cgtcgataca ggaaggtaga
      661 cgccgacggc gaatggcagc gacggaatct tgagcagctc gcaggccagc gcgataccaa
      721 taccgatcgc aacaaatccc cacggcaact gctggtcgag aacaccctcg atgacgagtt
      781 tcatcaaggt cgcctgtgga gccggaagct ccgttccgcc aaagccgaat gtctccgcaa
      841 gcagcagcac cgaaaggcag acgaatgttg ccgatgtcaa cacgccgaca agctcgccca
      901 cctgctggcg ccgcggtgtt gcgccgagaa ggtagccggt cttcaggtcc tgggaagtat
      961 cgccggcgat cgaggccgcg atcgcgacga cgcagccgac agtgagcgca gccgccttcc
     1021 cggtcatgtc ggtccagccc atcaccagga aaacgccggc ggtaccgagc agcgaagcga
     1081 tggtcatgcc gctggtcggg ttgctggtga cgccaaccaa tcccacgatg cgcgaggcga
     1141 cagtgacgaa gaaaaacgcg aacaccacca cgcacagcgc tgcaatcaaa cgcacgccga
     1201 ggccgccaac gtcgccaaag acctgcggca cgagaccgag aaacagcacg atggccagca
     1261 agccgatgcc gatgaacttc aacggcaggt cgttatcggt gcgcggtacg gcagcccgta
     1321 ttgtgccatc atcgccgacg cgttcgcgaa tctgtgccgt gccgatgcga aagctctgca
     1381 ccatgaccgg gatgctgcgg atcagggtaa cgatgcctgc ggtagccacc gctcccgctc
     1441 cgatgtacct gacatagcgg ctccacagtt cgctggccga catgtcgcgg atcagcgccg
     1501 tgctttccgg gaacaccggc gcgatgaagc cgtcacccca ccacgcaatc cccgggatga
     1561 ttagcaccca tgccagcagt ccgccgccca ccatcacggc cgcgatgcgc ggacccagaa
     1621 tgtaaccgac gccgaacaac gcgggcgaca ggtccatccc gagttcgccc ttcctgatga
     1681 acgggagccg cgtccatgcg gcctccggga tgatgtgcag ccacgaggtc aggaacttga
     1741 agaacgcacc aagtcccagg ccatagaaca cgtagcgcgc actggatccc ccaacctcgt
     1801 ttgcaaccag cacctccgca caggcggtgc cctccgggta cgggaggcgg ccatgctctc
     1861 ccttgatcag gaagcgcctc agcggaatca tgaacaggac gccgaggagc cctccgcaca
     1921 tcgccagcag cgtcatctgc aacagtcccg gcgacaggcc ccacaggaac agcgcgggca
     1981 gcgtaaaaat gacgccgctt gccagcgaac tcgacgccga gccaaccgtc tgcgcgaggt
     2041 tggcttccag aatcgtgccg gtcttgccga ccgtctgcag cgccctgaac gcggcaacgg
     2101 tcatgaccgc cacggggatc gaggtgctga tggtcaggcc ggcgcgaagc ccgaggtagg
     2161 cattcgcggc gccgaagacg atgccgaaca ggatgccgaa aaacgcggcc ttgatcgtga
     2221 attcggcgat ggagtcttcg gcgggaacgt agggaggata ttcctgccc
//
LOCUS       DASUOM010000551         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1641713,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000551 DASUOM010000000
VERSION     DASUOM010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641713"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFY27_11470"
     CDS             complement(<1..731)
                     /locus_tag="VFY27_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5766179.1"
                     /translation="MSLKLEITSTHKDLMGDDHICEFNAGGGTIGRGLENDWVLPDRD
                     RFISSRHATIDFRSGAYYLADTSTNGVYINQEDEPIGRRHPRRLFNGDRLRMGDFLIS
                     VSVDEGEDLDIPAEAPRAPVNHLELMVPEEPIKTGIQLLGEEEITGGDVFESTLFGKQ
                     TQKMTRNTASPAKAKQPPIAKPKPAPVRRPAVRRHADGGKLDAEQLFEEFLAGLGIER
                     ADLHPSADPGEIMLNAGQVLREFVAG"
     gene            856..1200
                     /locus_tag="VFY27_11475"
     CDS             856..1200
                     /locus_tag="VFY27_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEX5766180.1"
                     /translation="MPEKRLYKIMFMSQGQVYEIYARNVGHGSLFGFVEVEKLIFGER
                     SSVVVDPSEEKIKTEFGGVKRTYLPMHSIIRIDEVDKQGTSKISRLEGGNVAQFPVPV
                     YTPGGSGGSNKS"
     gene            1214..>3780
                     /gene="purL"
                     /locus_tag="VFY27_11480"
                     /gene_synonym="purI"
     CDS             1214..>3780
                     /gene="purL"
                     /locus_tag="VFY27_11480"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX5766181.1"
                     /translation="MSRPATEPLGESSIAAILQLPGPEAWSGFRLERKLETLRREVPP
                     VTALHSGYHYFVHCSGELSAAEHLRLADLLPGANTGRDQPQEPHFFVVPRAGTISPWS
                     SKATDIAHACGFSKVKRIERGIACSVDSLEPLSSRELQAAGSVLSDRMTEMLLFRAAD
                     AKQLFVTEGPRSFEMIPLTHRGRAALVEANRALALALSADEIDYLHENYARLQRDPTD
                     AELMMFAQANSEHCRHKIFRADWIIDGVPQKAKLFDMIRSTTEASRRGVISAYSDNAA
                     VIEGSRGLKLIPQLPERRYDWSEEPIHILLKVETHNHPTAISPFPGAATGAGGEIRDE
                     GATGLGARPKAGLTGFTVSHLRIPGFGQPWEKARAHPARMATPLQIMLDGPIGAASFN
                     NEFGRPNLAGYFRTFEADLPGLPGGEFRGYVKPIMVAGGLGNVRAAHARKVEVPAGAR
                     IVVIGGPAMLIGLGGGAASSLASGSSTEELDFASVQRGNPEMQRRAQEVIDRCTALGE
                     ENPILLIHDVGAGGLSNAVPEAVDHSKLGARVDIRSVPNAEPGMSPLGIWCNEAQERY
                     VLIVAEHRIPGFSALCERERCPFAVIGTLTSDQRLLVTDSEFDNSVIDMPMQLLLGKP
                     PKMTRDVARLPKQQVELDLRGVGLDDAVTRVLRFPAVADKSFLIHIGDRTVGGLSVRD
                     QMVGPWQVPVSDVAITAGGFENEAGEAMAMGERTPLAILNAPASGRMAVGEAITNIVA
                     ASIEDLQQVRLSANWMAAAAHAGEDAELFDTVKAVSHDLCRELGIAIPVGKDSLSMRA
                     RWMDESGEHVSVAPVSLIVSAFAPVSDVRSHLTPQLRRAREPTLLLLLDLGHGANRLG
                     "
BASE COUNT          697 a         1180 c         1214 g          689 t
ORIGIN      
        1 ccggcgacga actcgcgcag cacctggcct gcgttcagca ttatctcccc cggatcggcg
       61 gaaggatgca aatctgcacg ttcgatgccg agtccggcca ggaattcctc gaacagctgc
      121 tcggcatcga gcttgccgcc atcggcatgc cggcgcaccg ccggtcgacg cacgggtgcg
      181 ggcttcggct tggctatcgg cggctgcttt gccttcgccg gcgatgcggt gttgcgcgtc
      241 atcttttgcg tctgtttgcc gaagagcgtc gactcgaaaa catccccgcc ggttatttcc
      301 tcctcgccca gcaactggat gccggtcttg atcggctctt ccggcaccat cagttcgaga
      361 tggttgacag gagctctcgg cgcttccgcg gggatgtcga gatcctcgcc ttcgtcaacg
      421 ctgacggaga tcaggaagtc gcccatgcgc agccggtcgc cgttaaacaa gcgccgcgga
      481 tggcgcctgc cgatcggttc gtcctcctgg ttgatataca cgccgttcgt gctcgtatcc
      541 gcgaggtaat acgcgccgct gcgaaaatcg atggtcgcgt gccggctgga aatgaagcgg
      601 tcacggtcag gcagtaccca gtcattttcg agcccacggc cgatggttcc gccacctgca
      661 ttgaattcgc agatgtggtc gtcgcccatc aggtccttgt gcgtgctggt tatttcgagc
      721 ttgagcgaca ttgatatccc cggaaaatgg gaggccggca aggcgtcgat gcggtgccgg
      781 tctcgacctg taaattttgc aggggatcaa agctttgaac tgccaatcag ttcacactac
      841 tgtagacttc gcggcatgcc ggaaaagcgt ttgtacaaaa tcatgttcat gagccagggc
      901 caggtttatg agatctacgc ccgcaatgtc ggccacggat ccctgtttgg cttcgtcgag
      961 gtcgaaaagc tcattttcgg cgagcgaagt tccgtggtcg tcgatccctc cgaggaaaaa
     1021 atcaagacgg aattcggggg tgtgaaaagg acatacctgc cgatgcactc gatcatccgt
     1081 atcgacgagg tcgacaagca gggcaccagc aagatctccc gcctggaggg aggcaatgtt
     1141 gcgcagtttc cggtgcccgt ctacaccccg ggcggcagcg gcggcagcaa caagagctga
     1201 ggttcagaag cgcttgtcca gacctgcgac cgagcctttg ggagaatcgt cgatcgctgc
     1261 aatcctgcag ctgccaggac ccgaggcgtg gtcgggcttc cggctggaaa ggaaactcga
     1321 gaccctgcgc cgggaggtgc cccctgtcac cgcgctgcat tcgggctacc actacttcgt
     1381 ccattgttcc ggcgagctgt ctgccgcgga acacttgcgc cttgcggact tgctgcccgg
     1441 cgcgaacacc ggccgcgatc aaccacagga gccgcacttc ttcgtggtgc cgcgggccgg
     1501 cactatctcg ccctggtcca gcaaggcaac ggatatcgcc catgcctgcg gcttcagcaa
     1561 ggtcaaacgc atcgagcgcg gcattgcctg ctctgtagac agcctcgagc ctctctcatc
     1621 gcgggaactg caggccgcag ggagcgtgct gagcgatcgt atgaccgaga tgctcctttt
     1681 tcgcgcagca gatgcaaaac aactgttcgt aaccgagggc ccgcggtcgt tcgagatgat
     1741 accgttgacg caccgcggga gggcagccct cgtcgaggcg aatcgagccc tcgcacttgc
     1801 gctgtctgcc gatgaaatcg actacctgca cgagaactac gcccgcctgc aacgcgatcc
     1861 aacggacgcc gaactcatga tgttcgcgca ggcgaattca gaacactgcc ggcacaagat
     1921 ttttcgcgcc gactggatca tcgacggggt gccgcagaag gcgaagctgt tcgacatgat
     1981 tcgcagcacg accgaagcga gtcgacgcgg tgtgatctcg gcctactcgg acaatgcggc
     2041 ggtgatcgaa ggctccaggg gcctgaagct gatcccgcag ttgccggagc gccgctacga
     2101 ctggtcggaa gagccgatcc acatcctcct gaaggtcgag acgcacaatc acccgactgc
     2161 catttccccg tttcccggag ctgcaaccgg cgcgggagga gagatccgcg atgaaggcgc
     2221 cacggggctg ggcgcgagac cgaaggccgg gcttaccggc ttcacggtgt cgcacctgcg
     2281 tatcccgggc ttcggtcagc catgggagaa ggcacgtgcg catccggcgc ggatggctac
     2341 gccgttgcag atcatgctcg acggcccgat cggagcggca tcgttcaaca acgagtttgg
     2401 ccggccgaac ctcgcgggct acttccgcac cttcgaggcc gacctgcccg gactgccggg
     2461 cggggaattt cgcggttacg tcaagccgat catggttgcc ggcggcctcg gcaacgtgcg
     2521 cgccgcgcac gcccggaagg tagaggtccc ggccggcgcg cggatcgtgg tgatcggagg
     2581 gccggcgatg ctcatagggc tcggtggcgg ggcggcgtcc agcctggcga gcggcagcag
     2641 caccgaagag ctggatttcg cgtcggtaca gcgcggcaat cccgagatgc aacgccgcgc
     2701 acaggaagtt atcgatcggt gcacggctct cggcgaagag aacccgatat tgctgattca
     2761 cgatgtaggc gcaggcggcc tgtcgaacgc cgtgcccgaa gccgtcgacc acagcaagct
     2821 tggcgcgcgg gtggacatcc gttcggtacc caatgcggag cccggaatgt cgccgctcgg
     2881 aatctggtgc aatgaggcgc aggaaagata cgtcctgatc gtcgctgagc atcgaattcc
     2941 agggttcagc gcgttgtgcg agcgggaacg ctgcccgttc gccgtcatcg gcacgctcac
     3001 gagcgatcag cgcctcctcg ttaccgacag cgagttcgac aacagcgtca tcgacatgcc
     3061 gatgcagctg ctgctcggca agccgccgaa gatgacccgc gacgttgcgc ggctgccaaa
     3121 gcagcaggtc gaactggacc tccgcggcgt cgggcttgac gatgccgtca cacgagtgct
     3181 gcggtttccc gccgttgccg acaaatcgtt cctgattcac atcggcgatc gcactgtcgg
     3241 cggcctgagc gtgcgcgacc agatggtcgg gccctggcag gtgccggtca gcgatgtcgc
     3301 gatcacggcc ggcggtttcg agaacgaagc cggcgaggcc atggcaatgg gcgagcgcac
     3361 gccgctggcg atcctgaacg ccccggcatc gggacgtatg gctgtcggag aggcgatcac
     3421 gaatatcgtc gccgcttcta tcgaggatct gcagcaggtg cggctttcgg ccaactggat
     3481 ggctgccgca gcgcacgccg gcgaagacgc cgagcttttc gacaccgtca aggccgtcag
     3541 ccacgacctc tgccgggaac tgggcatcgc gatcccggtg ggcaaagatt cgctgtccat
     3601 gcgggcgcgc tggatggacg agagcggcga gcacgtttca gtagcgccgg tatcgctgat
     3661 cgtgtcggcg ttcgctccgg tcagcgacgt gcgcagccac ctgacgcccc agcttcgccg
     3721 cgcaagggaa cccacgctgc tgctgttgct cgaccttggt cacggcgcca atcgcctcgg
//
LOCUS       DASUOM010000552         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_71286,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000552 DASUOM010000000
VERSION     DASUOM010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71286"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFY27_11485"
     CDS             <1..510
                     /locus_tag="VFY27_11485"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX5766182.1"
                     /translation="GVLGMSVAHMGLCIFVLGVTITSAFSVESDMRMASGEKVEVAGY
                     EFELLGLRNVQGPNYRALEGQVEVRKDGEFVAVLRPQKRTYLVQQSPMTEAGIDSALG
                     RDLFVALGDPLGGGAWSVRLQYKPMIVFIWLGALVMALGGMIAASDRRYRVNVVVRER
                     RKARVEKPA"
     gene            507..1037
                     /locus_tag="VFY27_11490"
     CDS             507..1037
                     /locus_tag="VFY27_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5766183.1"
                     /translation="MRLRYLGPIIALGILIPIFVIGLQRDPSKVPSPYLGKQAPAFSL
                     PSVEDPAAIVSSENYKGQVALVNVWATWCVGCRHEHDFLLTLAKREDVPIYGLNWRDD
                     REEAVLWLQQLGDPYVASAFDADGNVGIDWGVYGAPETFLIGPDGTVLHKHLGPLTEE
                     SWQRDFVPKIAAVRGN"
     gene            1037..1471
                     /locus_tag="VFY27_11495"
     CDS             1037..1471
                     /locus_tag="VFY27_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX5766184.1"
                     /translation="MRAFTAALVLLFAPAAFAIDAGVAFDDPVLQARYEEIISEVRCL
                     QCQNESLKDSNAFLAVDLRREIRRLLSEGKSNAEIYDFLVQRYGEFALYRPRMSGKTL
                     FLWLAPLLLIAAGSVVAFRVVRARMALPVADDDDEVQLTDRT"
     gene            1468..2451
                     /locus_tag="VFY27_11500"
     CDS             1468..2451
                     /locus_tag="VFY27_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5766185.1"
                     /translation="MIWLLFGAMLLAAALAVTWPMYRVEKRVTSSVIASVVVVLGVSA
                     VIYSSIGTPVPPEPVASLEDAAAALEERLQEHAADIDGWKMLGRSYMQLQNYSKAITA
                     FEKVAELEATPTAQTMASLGEALLIEEGSASADKVSQLFESALSLEPANPKALFYGGI
                     IAIERGNRDLAADRWEALLALSPPPEIEDVLRQRVAEWRGEIPAVTQAGPASSEAIVA
                     VNVSLAPGAASAVAPDATVFIIARDPAQPSPPIAAARRRVSELPAQVSLSDADAMIPG
                     RPLSAFDELEIVARVSMSGAPAAQTGDWYGEHTVSQPGSAPVDIVIGKQVP"
     gene            2496..2861
                     /locus_tag="VFY27_11505"
     CDS             2496..2861
                     /locus_tag="VFY27_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEX5766186.1"
                     /translation="MCFACGRDNPVGLKIHFQLQGERCVGEFTPSANHVGYRNTVHGG
                     IIYAALDDVMANFLYLQNIKAHTARCEVRYRKPLEVGQTIDLAGWVARRRGRLVILQG
                     EARHADALIADCEASFMLV"
     gene            complement(2867..3097)
                     /locus_tag="VFY27_11510"
     CDS             complement(2867..3097)
                     /locus_tag="VFY27_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766187.1"
                     /translation="MAGRSTRETDIHDLSLSVIHALAMVDVKVLSYIQLRRLYAALTH
                     ARDDVEQETLRRTDGNIYGDVVNVPPPEPLDE"
     gene            complement(3163..4512)
                     /locus_tag="VFY27_11515"
     CDS             complement(3163..4512)
                     /locus_tag="VFY27_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5766188.1"
                     /translation="MPFRHQRELELLRQALDTLQAGFGGLADSASHYDLSGVADVLQE
                     TARRMQDNYPYHHPRYAGQMLKPPHPVARLAYALSLWLNPNNHALDGGRASSAMELEC
                     VAAIARMFGMREWLGHLCGGGTMANLEALWVAGQLRPGCILASRQAHYTHARLSEVLK
                     LDFASVPVTPAGGLDLEALRGLLRTRQVGTVVATIGTTGVGAIDPLADILALKEEFDF
                     RVHVDAAYGGYFQIAAELGDASRRHYDCIAKADSIVIDPHKHGLQPYGCGCVLFKEPG
                     VGTLYKHDSAYTYFSSSELHLGEISLECSRPGAAAVALWATQQLLPMQRGAAFAEGLD
                     RSLLAARKFHQWIEASENYSALMPPELDIVVYAVNAPDAVTASNRARAIFERAAQANL
                     HLALIELPAAIVGHYRPELEVNRDTVTCLRSCLMKPEHLDWLDDICTSLDTLAERLT"
     gene            complement(4517..>5516)
                     /locus_tag="VFY27_11520"
     CDS             complement(4517..>5516)
                     /locus_tag="VFY27_11520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5766189.1"
                     /translation="LDEFAGWLNIAEQHGLDTRIITGRELEEQVGPAASQWLGALYTA
                     SDGRAEPHKAAPAIARAAAAKGAKILTGCAVRGLESEAGRISAVVTEHGRIRTSTVLC
                     AAGAWSSLFCRSLGISVPQLKVQGNVLRTAPARAVLSGNVFDCKVGIRRRQDGGYTVA
                     HGAILMHAITPSTFRYAFKFLPALRQEIKVLRLSIGREFLTEFRMPSRWALDSVSPFE
                     KNRVLNPKPSPSVIAKIKVNLAETFPELADTPIAETWAGMVETSPDVVPMICPIERYP
                     GFYLATGFSGHGFGLGPGAGKAIAGMLTGRDSGIDLRQLRLERFFDGSPIRPQSTV"
BASE COUNT          984 a         1652 c         1828 g         1052 t
ORIGIN      
        1 ggcgtgctcg gcatgtcggt tgcgcacatg gggctctgca tcttcgtcct cggtgtcacg
       61 atcacatccg cgttcagcgt cgagtccgac atgcgtatgg cgtccggcga aaaagtcgag
      121 gttgccgggt atgaattcga actgctcggc cttcgcaacg tacagggacc gaactatcgc
      181 gcgctggagg ggcaggttga agtccggaag gacggtgaat tcgtggcggt tcttcgcccg
      241 cagaagcgaa cctacctggt gcagcagagc ccgatgacgg aggccgggat agattcggcg
      301 ctgggccgcg acctcttcgt cgcgctgggc gatcccctgg gcggcggcgc atggagcgtc
      361 cgcctgcagt acaagccgat gatcgttttc atctggctcg gtgcgctggt gatggcgctg
      421 ggcggcatga tcgccgcgag cgaccggcgc taccgcgtca acgtggtggt gcgcgaacgg
      481 agaaaagccc gagtggagaa gcctgcgtga gactgcgtta cctcgggccg ataatcgcgc
      541 tgggcatcct gatcccgatt ttcgtcatcg gcctgcagcg cgacccttcg aaggtgcctt
      601 caccgtatct cggcaagcag gcgcctgcgt tttcgctgcc cagcgtggag gatcctgccg
      661 ctatcgtcag cagtgagaat tacaagggac aggttgccct cgtaaacgtc tgggcgacct
      721 ggtgcgtagg ctgccggcat gagcatgatt ttctcctgac gcttgcgaag cgtgaggatg
      781 ttccgatcta tggcctcaac tggcgggatg atcgagagga agcggtgctc tggctgcagc
      841 aacttggcga cccgtatgtt gcttccgcgt tcgatgccga tggcaacgtc gggatcgact
      901 ggggtgttta cggtgcgccc gagacattcc tgataggtcc ggacggcacg gtgctgcaca
      961 agcacctggg gccgctgacg gaagagtcct ggcagcggga ttttgtgccg aagattgccg
     1021 ctgttcgtgg aaactgatgc gggcattcac ggcagccctt gttcttcttt tcgcgccggc
     1081 cgctttcgcg atcgatgcag gtgtggcgtt cgacgacccg gtactgcagg cgcggtacga
     1141 ggagatcatt tcggaggtgc gttgcctcca gtgccagaac gaaagcttga aggactcgaa
     1201 tgcgttcctt gcggtagacc tgcggcgcga gatccggcgc ttgctgagcg aaggcaagag
     1261 caatgcggag atttacgact tcctggtaca gcgttacggg gagttcgcac tctatcgtcc
     1321 gcgcatgagt ggcaagacgc tgttcctgtg gctggcgccg ctgttgctga tcgccgcggg
     1381 cagcgtggtt gcgttccggg tcgtacgggc acgcatggcg ctgccggttg cggatgacga
     1441 cgatgaagtc cagctgacgg accgcacgtg atctggttgt tattcggcgc aatgcttctc
     1501 gcagccgcgc tcgcggtcac gtggccgatg taccgcgtgg agaagcgggt aaccagtagc
     1561 gtgatcgcgt cggttgtcgt agtgctgggc gtttctgcgg tcatctactc gagtatcggc
     1621 acaccggtgc cgcctgagcc tgtggccagc ctcgaggatg cggcagctgc gctcgaggag
     1681 cgcctgcagg aacatgccgc tgacattgac ggctggaaaa tgctcgggcg ctcgtacatg
     1741 cagctgcaga attactcgaa ggcaatcacc gcgttcgaaa aggtagccga actcgaagcc
     1801 acgcccaccg cacaaaccat ggcgtctctc ggcgaagcgc tgctgatcga ggagggcagt
     1861 gcaagtgcag acaaggtgtc ccagctgttc gaatcggcgc tttcgctgga acctgccaat
     1921 ccgaaagcct tgttttatgg aggcatcatc gccatcgagc gcggcaacag ggatctcgcc
     1981 gctgatcggt gggaagcact cctcgctttg tcaccgccgc ccgagataga ggacgtcctc
     2041 cgccagcgag ttgcggagtg gcgcggcgaa attccggcgg tgacgcaggc gggccctgct
     2101 tcctccgagg caatcgtggc cgtaaacgtg tcgctcgccc ccggcgcggc ctccgcagtc
     2161 gcccccgatg ccaccgtatt catcatcgca cgggacccgg cgcagccttc ccctccgatc
     2221 gctgccgcca ggcgcagggt atcggagctg ccggcgcagg tgtcgctgag cgacgctgac
     2281 gcgatgatac cgggacgccc gttgtcggca ttcgacgagt tggagattgt cgcgcgcgtg
     2341 tcgatgtcgg gcgcccccgc agcgcagacc ggtgactggt atggcgaaca caccgtgtcg
     2401 caaccgggtt ccgcgccggt cgatatcgtc atcggcaagc aagtgccgta gcggcagatc
     2461 acttgagcgc tccaggaacg attgaagcag ccacgatgtg cttcgcgtgc ggccgggata
     2521 atccggtcgg cctgaagata cactttcaat tgcagggaga gcgctgcgtc ggcgagttca
     2581 caccgtcagc gaaccacgtg ggctaccgga atacggtgca cggcggcatc atttacgccg
     2641 cgctcgatga cgtgatggcg aattttctgt acttgcagaa catcaaggcg cacacggcgc
     2701 gttgcgaggt tcggtatcgg aagcctttgg aggtcgggca gacgatcgac ctcgcggggt
     2761 gggttgccag gcgtcgtgga cgtttggtga tcctgcaggg cgaagcgaga cacgcggatg
     2821 cgctcattgc cgattgcgaa gcgagcttta tgctggtgtg acgcttttat tcgtccaggg
     2881 gttccggtgg ggggacgtta accacgtcgc cgtagatgtt gccgtcggtg cgcctgaggg
     2941 tttcctgttc gacgtcgtcg cgcgcgtgcg tgagggcggc atagaggcga cgaagctgaa
     3001 tataggacag gactttcaca tccaccatcg ccagcgcatg gatgacgctg agtgacaggt
     3061 cgtggatgtc cgtctcccgg gtactccgtc cggccatgat cgggcccttg catgcctggt
     3121 ctgcagtcta gcagcggtga aggtagcgaa atgtgacggg cgtcacgtca gtctttcggc
     3181 gagcgtgtcg agtgacgtgc agatgtcatc gagccaatcc aggtgctcgg gcttcatcag
     3241 gcacgaccgc aggcaggtga cggtgtcacg gttcacctcg agctccggac ggtagtgacc
     3301 gacgatagct gcagggagct cgatcagggc gaggtgcagg ttcgcctgcg ccgctcgctc
     3361 gaagatcgcg cgggcgcggt tgctggcagt gacggcgtcc ggcgcattaa cggcatagac
     3421 cacgatgtcc agttctggcg gcatcagggc cgagtagttc tcactggcct ctatccattg
     3481 atggaacttc cgggcagcca gtaatgaacg atcgagacct tcggcaaagg cggcgccgcg
     3541 ctgcatgggc agcaattgct gcgttgccca caacgccacc gcggcggcgc ctggccgtga
     3601 gcattccagg ctgatctcgc ccaggtggag ctccgatgac gagaagtagg tgtaggcaga
     3661 atcgtgcttg tacagggtcc cgacacccgg ctccttgaac agcacgcacc cgcatccata
     3721 cggttggagt ccgtgcttgt gcgggtcgat gacgattgaa tccgcttttg cgatgcagtc
     3781 atagtgccgc cggctcgcgt cgcccagttc agcggcgatt tgaaagtagc cgccatacgc
     3841 tgcatccacg tgtacgcgaa aatcgaactc ctctttgagc gcaagtatgt cggcaagcgg
     3901 atctatagcg ccgacaccgg tcgtgccgat ggtcgcgacc actgtcccga cctgtctcgt
     3961 ccgcaaaagg ccgcgtaacg cctccaagtc caggccgcct gcgggagtca cggggacact
     4021 cgcgaagtcg agcttgagca cttcactgag acgcgcgtgc gtgtaatgcg cctgccggga
     4081 ggcgaggatg cagcccggcc gcagctggcc cgccacccac agagcttcca aattcgccat
     4141 cgtaccgccg ccgcacaagt ggccgagcca ctcgcgcatg ccgaacattc gcgcgatggc
     4201 cgcgacgcac tcgagttcca tggcggaact cgccctgccg ccatccagcg catggttgtt
     4261 cgggttgagc cagagcgaaa gggcgtaggc caggcgcgcc acgggatggg gcggtttcag
     4321 catctgtcct gcgtaacgcg gatggtggta cgggtagttg tcctgcattc ggcgcgctgt
     4381 ctcctgcaga acgtccgcga ctcccgaaag atcgtagtgc gaagcgctgt ccgccaggcc
     4441 gccgaagccg gcctgcagcg tgtcgagcgc ctgcctgagg agctcgagtt cgcgctggtg
     4501 ccggaacggc attgcttcag accgtcgact gcggacggat cgggctgccg tcgaagaagc
     4561 gctcgagtcg cagctggcgc aggtcgatgc cggagtctct acccgtcagc atgcccgcga
     4621 tcgccttgcc ggcgccgggg cccaggccga atccgtgccc gctgaaccca gtcgcgagat
     4681 agaacccggg gtaacgctct atcgggcaga tcatcggaac gacgtccgga gatgtttcca
     4741 ccatgccggc ccaggtctcg gctatcggag tgtcagcaag ctccggaaac gtctccgcga
     4801 ggtttacctt gatcttcgca atcactgaag gagaaggttt cgggttgagc acgcggttct
     4861 tctcgaacgg agaaacgctg tcgagcgccc atcgcgaagg catgcggaac tcggtgagaa
     4921 actcccggcc aatggacaac ctgagcactt tgatttcctg ccgcaacgcg ggaaggaatt
     4981 tgaacgcgta gcgaaacgtg gatggcgtaa tcgcatgcat caggatcgcg ccgtgcgcca
     5041 ccgtgtaacc gccgtcctgc cggcggcgaa tgccgacctt gcaatcgaac acgttgccgc
     5101 tcaagacggc tcttgccggg gcagtacgca ggacatttcc ctggaccttg agctgcggca
     5161 ccgagatgcc gagcgagcga cagaaaagcg acgaccacgc accggccgcg cacagaacgg
     5221 tggatgtcct gatcctgccg tgttcggtga cgacagccga aatgcggccg gcttcggact
     5281 ccaggccgcg caccgcgcag cccgtcagga ttttcgctcc cttcgccgcc gcagcccgcg
     5341 cgatcgccgg cgcggccttg tgcggttcag cgcgaccgtc gcttgccgta tacagggcgc
     5401 ccagccactg cgacgcggcg ggacccacct gttcttccag ttcccggccg gtgatgatgc
     5461 gggtatcgag gccgtgctgc tccgcgatgt tcagccatcc ggcaaactca tccagt
//
LOCUS       DASUOM010000553         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_35573,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000553 DASUOM010000000
VERSION     DASUOM010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35573"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFY27_11525"
     CDS             <1..299
                     /locus_tag="VFY27_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5766190.1"
                     /translation="GPDADDDQMFTPAAYLPSPDSDPAVLMEDMDWHDDATSRMSEAL
                     TNLDDRSRHILETRWLADEKQTLHELADEYGVSAERIRQIENNAIKKLRVAMEA"
     gene            complement(386..703)
                     /locus_tag="VFY27_11530"
     CDS             complement(386..703)
                     /locus_tag="VFY27_11530"
                     /inference="COORDINATES: protein motif:HMM:NF023374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HEX5766191.1"
                     /translation="MNRILNEFDTGTWIEGTGLVRRLIQDDNNGARHQRFILDLRNGQ
                     SLLMAHNLDLAERVPAGLGDRVGFRGMYEWNELGGLVHWTHHDPLGIEDGGFIRYRGK
                     VYK"
     gene            complement(707..1375)
                     /locus_tag="VFY27_11535"
     CDS             complement(707..1375)
                     /locus_tag="VFY27_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5766192.1"
                     /translation="MKSIAVAAIAALTTLGIAGCETLDAYTQESKTSSTAKGAMIGAA
                     AGAAVGLMSGDDAVERRQQAMILAGVGALAGGAVGNYMDRQESKLRAELQGTGVSVTR
                     IGDNITLNMPGNVTFATDSSDLSPAFFDVLNSVGKVMKEYNKTVVEVAGHTDSTGSDS
                     YNQSLSERRAGAVAQYLRSQQIGSERLITVGMGETRPVADNGSADGRQANRRVEITMV
                     PLTS"
     gene            1637..>2002
                     /locus_tag="VFY27_11540"
     CDS             1637..>2002
                     /locus_tag="VFY27_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766193.1"
                     /translation="MRETDRWLSDYGDSHRNISCPAIYWPAVLVLVPGITGMLWSLPT
                     PAAFARISPLLNWGSAFLMASLVYYFIISLPLAIGMLPFMVSVAAMGVLLAQAEVSVA
                     GVSLGVVVVSLAGLYFGHHA"
BASE COUNT          369 a          618 c          588 g          427 t
ORIGIN      
        1 cgggtcccga cgctgacgat gatcagatgt tcactcccgc agcctacctg ccgagccctg
       61 actcggaccc ggcggtgctg atggaagaca tggattggca cgacgacgcc accagccgca
      121 tgagcgaggc gctcacgaat ctcgacgacc ggagccgcca catcctggaa acgcgctggc
      181 tcgccgacga gaaacagaca ctgcatgaac tggccgatga gtacggcgtg tcggcggaac
      241 gcattcgcca gatcgagaac aacgcgatca agaaactgcg cgtggcgatg gaagcgtaat
      301 tggcttcttt gctctgctcc tggccgtagc gagaattcgg aagagaaacc tgcaggagca
      361 tcgatcagaa gctgcacaag cttcgctact tgtagacttt tccgcggtaa cgaataaagc
      421 cgccgtcttc gatgccgagc gggtcgtggt gcgtccagtg caccagcccg ccaagctcgt
      481 tccactcgta catgccgcga aagccgacgc gatcgccgag tcctgcgggc accctctccg
      541 caagatccag gttgtgggcc atcagcaggc tctgcccgtt gcgcaggtcc aggatgaaac
      601 gctgatgacg tgcgccgttg ttgtcatcct gaatgagcct gcgcacgaga cctgtccctt
      661 cgatccaggt cccggtgtcg aactcgttca ggatgcggtt caagcgttag ctggtaagcg
      721 gcaccatcgt aatctctacg cgccggtttg cctgccgtcc gtcagcgctg ccattgtcag
      781 cgacgggacg tgtctcaccc atgccgaccg ttatgagtct ttcgctgccg atctgctgtg
      841 accgaaggta ctgcgcgacc gctccagcgc gacgctcgga gagactttgg ttgtaggagt
      901 cgctgcccgt gctgtcggta tgacctgcga cctcgaccac cgtcttgttg tactccttca
      961 tcaccttccc gacggagttc agcacatcaa aaaaggcggg gctcaggtcg gaactgtccg
     1021 tggcaaaggt gacattgcct ggcatgttga gtgtaatatt gtcgcctatg cgtgtaacgc
     1081 tgacgcccgt tccctgcagc tccgcgcgca gcttgctctc ctggcgatcc atgtagttgc
     1141 ctactgcacc tcccgcaagc gcgccgacac cggcgagaat catcgcctgt tgcctgcgtt
     1201 caacggcatc gtcgcctgac atgagtccga ccgctgcgcc ggcggccgca ccgatcatgg
     1261 cgcccttcgc cgtgctgctg gttttcgact cctgcgtata cgcatccagg gtttcgcatc
     1321 cggcgattcc gagggtcgtt aatgctgcaa tggcagcgac tgcaatggat ttcatgttca
     1381 tcataattcc ctgtaactga ctgcccaatg aaacggtgac gtggcgggag cgttcctccg
     1441 attgaatgcc cgcgcctgaa ttcgagaggc gtattgtagc ggctcggaac ctgaacaagg
     1501 acttaaaaca gccgcccaaa gtggcgactg gttctcaaat cggagaacga atctcttcat
     1561 gaacactccg ggcgagggcc gaaggtgcag cggtgtaaac tattcgtcgc ctgccgatcc
     1621 ccagcgggga gtatccatgc gtgaaaccga tcgttggctg tcggattacg gcgacagtca
     1681 ccggaacatt tcctgtccag cgatctactg gcctgccgtt ctcgtcctgg tgccgggcat
     1741 caccggcatg ctgtggtcgc tgccgacgcc cgccgcgttc gcccggattt cgccgctcct
     1801 caactggggc agcgcgttcc tcatggcatc gctcgtctac tacttcatca tctccctgcc
     1861 gctcgcaata ggcatgctgc cgttcatggt gagcgttgcg gctatgggag tattactggc
     1921 gcaggcggaa gtttcggtcg cgggcgtgtc gctgggtgtg gtcgttgtct cgctggccgg
     1981 actctacttc gggcaccacg cg
//
LOCUS       DASUOM010000554         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1158403,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000554 DASUOM010000000
VERSION     DASUOM010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158403"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFY27_11545"
     CDS             <1..500
                     /locus_tag="VFY27_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766194.1"
                     /translation="ALAVASVNVERYGMQERVRLIQSNYFEKLAARTATGDGGYDLIV
                     SNPPYVDADEMAALPAEFAHEPALGLAAGEDGLDSVLAILHDACAFLSDEGILVVEVG
                     MSEQALQKEFPGIPFLWLDFESGGSGVFLLTREELTIHREKFAVAIRRRIDAGSGVKV
                     QPDVR"
     gene            490..1584
                     /gene="aroC"
                     /locus_tag="VFY27_11550"
     CDS             490..1584
                     /gene="aroC"
                     /locus_tag="VFY27_11550"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5766195.1"
                     /translation="MSGNSFGVLFSVTTFGESHGPAMGCIIDGCPPGLGLEAADIQAD
                     LERRRPGRSRHTTQRNEPDQVQILSGVFEGVTTGTPIGLLIENKDQRSHDYSEIKDKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETTMRVAAGAVARKYLRERLNVEITGYLAQL
                     GPIALEAVSLSAIDDNPFFCADPGRVRELEQFMDELREAGDSIGAKLSILARNVPPGL
                     GEPVFDKLEADLAKGLMSINAVKGVEIGAGFAAVSQRGSEHRDQMTPDGFDKNDAGGT
                     LGGISSGQDILASIALKPTSSIAIPGKTVDKAGQRSEIVTTGRHDPCVGLRAVPIAEA
                     MVALVLMDHFLRHRGQNADVASGTPVIPGR"
     gene            1760..2782
                     /locus_tag="VFY27_11555"
     CDS             1760..2782
                     /locus_tag="VFY27_11555"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5766196.1"
                     /translation="MAERYDVAVVGATGAVGEAMLEILAERKFPVGTVHALASERSVG
                     KEVRFGRQALRVAHLADFDFSQVQIGLFSAGGSVSAEYAPKAAAAGCVVVDNTSKFRL
                     EPDIPLVVPEVNPEKIADYRATGIIANPNCSTIQMVVALKPIYDAVGIKRINVATYQA
                     VSGAGRSAVEELVRQTSLLLNGRPLDMKGAARQIAFNAVPHIDEFQDNRYTREEMKMV
                     WETQKILGDETIAVNATAVRIPVFYGHSEVVNIETEKKISAAEVCDLMRGAQGVELID
                     GTKTGEYPTAVTDASGKDAVYVGRVREDLSHPHGINLWIVSDNIRKGAALNSVQIAEI
                     LAKNHL"
     gene            2916..>3997
                     /locus_tag="VFY27_11560"
     CDS             2916..>3997
                     /locus_tag="VFY27_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX5766197.1"
                     /translation="MSLRHNRGWLVLGLLLASDVWALGLGDIRLSSALNEPLRAEIQL
                     LSATPEELSNLKVELAPAATFDRYGIERPLFLTRLQFSVQQAGPNGPSTVRITSSEPL
                     TEPFVTFLVEATWSSGRLLREYTVLLDPPTFAPPPTSQSSQAVTAPSRAAQADSGRIE
                     RPAPQAAQPAAPRTAAAPADGGSFDTAEGGDVLVQRGDTLWEIAARVRPDNRLDMNQM
                     MMAIYEANPQAFAGNLNRLNAGATLRIPAADDIFRISRGDAMSEVQRQNSAWGMPDTR
                     TTQPSLTLVPPDSDQTGYESTSSQPSTATAGSAATADRIRQLEETIEEQNSLIEIRDN
                     ELAQLRDELAALRASQAAAADALPAGG"
BASE COUNT          832 a         1200 c         1285 g          680 t
ORIGIN      
        1 atgcgcttgc cgtcgcttcc gtgaacgtcg agcggtatgg catgcaggag cgcgtccgcc
       61 tcatccagtc aaattacttc gagaagctgg cggcccgaac ggcaacgggc gacggaggtt
      121 acgacctcat cgtcagcaat cctccctacg tcgatgcgga cgagatggcc gcgttgcctg
      181 ccgaattcgc acacgagccg gcgcttggcc tcgcggccgg cgaagacggt ctggattcag
      241 ttctggcgat actgcatgat gcgtgcgcat ttctctcgga cgagggaata cttgtggtcg
      301 aagtgggcat gagcgagcag gcgctgcaga aagagtttcc tggcatacca ttcctctggc
      361 tggatttcga aagcgggggc agcggcgtct tcctgctgac aagggaggaa ctgacgatcc
      421 atcgtgagaa attcgcggtc gccatccgcc ggcgcatcga tgcagggtcc ggagtaaagg
      481 ttcagcctga tgtccggtaa cagcttcggc gtcttgttct cggtgacgac gttcggcgaa
      541 agccacggcc ctgcgatggg ctgcatcatc gacggctgcc cgcccgggct cggactcgaa
      601 gcagccgata tccaggcgga cctggagcgg cgacgccccg gacgctcccg tcacaccaca
      661 cagcgcaacg agccggacca ggtgcagatc ctgtcgggag ttttcgaagg tgtgacgacc
      721 ggtacgccca tcgggctgtt gatcgagaac aaggaccagc gctcgcacga ctacagcgag
      781 atcaaggaca agtttcgtcc cggccacgcc gactacacct accagcagaa atacggcatt
      841 cgcgactatc ggggcggcgg ccgctcgtcg gcgagggaaa cgacgatgcg cgtggcggcc
      901 ggtgcggttg cgcggaagta cctacgcgag cgcctgaacg tcgaaatcac aggctacctg
      961 gcacagctcg gccctatcgc tctcgaggcg gtgagtttga gcgcgatcga cgacaacccg
     1021 tttttttgcg ccgatccggg cagggtgcgc gaactcgagc aattcatgga cgagctccgc
     1081 gaagcgggcg actccatcgg tgcgaaactc agcattctcg cgcgaaacgt ccccccgggg
     1141 ctgggcgagc cggtcttcga caagctggaa gcggatcttg cgaagggact catgagcatc
     1201 aacgccgtca agggcgtgga aatcggtgcg ggcttcgcgg ccgtcagcca gcgcggcagc
     1261 gagcacaggg accagatgac gccggacgga tttgacaaaa acgatgccgg cggtaccctc
     1321 ggcggcatct cgtcggggca ggacatcctg gccagcattg cccttaaacc gacctcgagc
     1381 atcgccatcc ctggcaagac agtggacaag gccgggcagc gctccgagat cgtcaccacc
     1441 ggccggcacg acccgtgcgt cgggttgcgc gccgtcccca tcgccgaggc gatggtcgcg
     1501 ctggtgctga tggaccactt cctgaggcac cgtggccaga atgcggacgt ggcgtcgggg
     1561 actcccgtaa taccagggcg ataatctttg gggaaacccc ggtgggccat tctgccggcg
     1621 tcaattggac aataattgcc ggaaccgtcg actgatcgtt atttttatgc acgcgtgctg
     1681 ccgacaatca ccctgatgga aggtgcagac tcgcgggaaa agagcgaaga agattcaaaa
     1741 tgaggaccaa gagaccctga tggcagagcg atacgacgtt gccgtagtcg gcgcgaccgg
     1801 agcagtgggc gaggcaatgc tcgagatact ggccgagcgg aaatttcctg tcgggacggt
     1861 gcatgcgctg gcgagcgaac gttcggtggg caaggaagtg cggttcggca ggcaagccct
     1921 gcgtgttgcg cacctggccg acttcgattt ttcgcaggtc cagatcggtc tgttctcggc
     1981 ggggggctcg gtttcggccg agtacgcgcc gaaggcagcc gcggcaggct gcgtcgtcgt
     2041 cgataacaca tcgaaattcc ggctcgaacc cgacatcccg ctggtcgttc cggaggttaa
     2101 cccggagaaa atcgcggatt accgggcgac cggcatcatc gcgaacccga attgttccac
     2161 gatccagatg gttgtggcgc tcaaacccat atatgacgcc gtcggcatca agcggatcaa
     2221 cgtcgcgacc taccaggcgg tttccggcgc gggccgcagc gcggtcgagg agctcgttcg
     2281 gcaaacatcc ctcttgctga acggccggcc gctcgacatg aaaggcgccg ctcgccagat
     2341 agcgttcaac gcggtgccgc acatcgacga attccaggac aaccgctaca cacgtgaaga
     2401 aatgaagatg gtgtgggaaa cgcagaagat actgggagac gaaacaatcg cggtgaatgc
     2461 aacggccgtg agaatcccgg tgttttacgg gcattccgaa gtcgtaaaca tcgagacgga
     2521 aaaaaagatt tcagcggccg aggtgtgtga tttaatgcgc ggtgctcagg gggtcgaact
     2581 gattgacggg acgaagacgg gagaataccc gacggctgtc actgacgcgt ccggcaaaga
     2641 tgccgtatac gttggccggg tgcgggaaga cctgagtcat ccgcatggca tcaacctgtg
     2701 gatcgtttcg gacaacatac gtaagggcgc cgcgctaaat agtgtccaaa tagctgaaat
     2761 attggccaaa aaccatttat aagctatagt tcgcgagcaa acgggatggc gtctgcaact
     2821 cattaaataa cctgcaaatt taactgccga aaatcaactg ccgggtgggg gtcctgattc
     2881 aagcccgggc aattggggag ctggagaaaa tagttatgtc gctaagacac aatcgcgggt
     2941 ggctcgttct cgggctgctc ctcgcaagcg atgtctgggc gctgggtctc ggagacattc
     3001 ggctcagttc ggccctcaac gagccgctca gggccgagat tcagttgctg tccgcaacgc
     3061 cggaagaact gtcgaacctg aaggtggagc ttgcgccggc ggcgaccttc gaccggtacg
     3121 gcatcgaaag gccgctcttc ctgaccaggt tgcaattttc ggtgcagcaa gcgggtccga
     3181 acggcccgag caccgtgcgc atcacgtcat ccgagcccct caccgagccc ttcgtcacct
     3241 tcctcgtgga agcgacctgg tccagcggca ggcttctgcg tgagtacacc gtgctgctcg
     3301 atcctccgac cttcgccccg ccgccgacct cgcagtcatc gcaggctgtg acggcgccga
     3361 gccgggcagc gcaggccgac agcggccgca ttgaacgtcc cgcgcctcaa gccgcgcaac
     3421 ccgctgcacc gcgaaccgcg gcggcgcctg cggatggcgg ctcgtttgac accgccgaag
     3481 gcggagacgt gctggtgcag cgcggcgaca cgctctggga gatcgcggcg cgcgtccggc
     3541 cggacaatcg cctcgatatg aaccagatga tgatggcgat ctatgaagcc aacccccagg
     3601 cattcgccgg caacctgaac cgcctcaacg ccggcgcgac tctcaggatc ccggccgccg
     3661 acgacatatt ccgcatcagt cgcggcgacg ccatgagcga agtgcagcgg cagaactctg
     3721 catggggcat gccggatacg cgcacgacgc aaccgagctt gacgctggtt cctccggact
     3781 cggaccagac aggctatgaa agcaccagct cgcagccttc gacagccact gccggaagcg
     3841 cggcaacggc ggatcgcatt cgtcaactcg aggaaacgat cgaagagcag aactcgctga
     3901 tcgagatccg cgacaacgag ctggcgcagc tgcgtgacga gctggcagcg ctgcgtgctt
     3961 cgcaggccgc cgcggcggat gcgctgccgg ccggcgg
//
LOCUS       DASUOM010000555         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_71863,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000555 DASUOM010000000
VERSION     DASUOM010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71863"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFY27_11565"
     CDS             complement(<1..256)
                     /locus_tag="VFY27_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766198.1"
                     /translation="MIAAVSTPEIGILYPGRILRSLFLATVLLFASCGGGETLMAEGP
                     ASHEDFARRIESNGLNNTIMGQRWLDAANAALTEPLPVVLP"
     gene            414..1319
                     /locus_tag="VFY27_11570"
     CDS             414..1319
                     /locus_tag="VFY27_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5766199.1"
                     /translation="MAADSQVSQLPPARIGISGWRYAGWRGKFYPEGLPQKNELAYAA
                     GRFNTIELNGSFYSLQRPESYAHWYRQVPDNFVFAIKGSRFITHMLRLRDVKTALANF
                     FASGLFELEEKLGPILWQLPPTFQYDRERLEAFFALLPKTGADASRLARKHDERIKAR
                     ARIRTDPNARLRYALEVRHKSFVKPDFINLLREHMIALVVADTAGKWPYMEDVTADFV
                     YVRLHGDKKIYESGYSGAVLDAWAKKVDAWRVGEEVADVHKVSAKRPPRKEAREVFVY
                     FDNDMKVHAPADAARLAKRLSARPA"
     gene            1376..1966
                     /locus_tag="VFY27_11575"
     CDS             1376..1966
                     /locus_tag="VFY27_11575"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5766200.1"
                     /translation="MSETRDRIVMAAMDLFWEKGYGSTSISDILKRAGANSGSLYHFF
                     PAKQDVLSAVLDTYHQGIGPMLLDPAWDGVDDPLDRIFVLLDRYRRFLIDTDYFYGCP
                     IGSLALELHEPDPPIRERLAANFSAWTAAIEECLDAAPDRLPPGVDKKALAQFVLTTM
                     EGGVMQARTYRSLESFDASVAQLRNYFEYLAGTAAS"
     gene            2052..2459
                     /locus_tag="VFY27_11580"
     CDS             2052..2459
                     /locus_tag="VFY27_11580"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX5766201.1"
                     /translation="MEDAMGQINYLAVIAAAVSAFLLGGLWYSALFGKAWMAASGVTK
                     ERAAEANQAKVFGVAFIWSLLGAYVFAMFLGPAPAFGFATAAGFAAGLFWVAGSFAIN
                     YQFEQRPARLLLINGGYHTLQYTLYGSILGLWH"
     gene            complement(2464..3123)
                     /locus_tag="VFY27_11585"
     CDS             complement(2464..3123)
                     /locus_tag="VFY27_11585"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5766202.1"
                     /translation="MYPDAPDDLSDRRHAMVATQIAARGVRSPKVLDAMRAVPREAFV
                     PEHMRKLAYQDSPLPIGAGQTISQPHVVALMIEALDLKGGENVLEVGGGSGYAAAVLG
                     QIAGRVCSIERIEKLAQSAKETLAALGYDNVEMRYGDGTRGCPDRAPFDAIVVAAGTP
                     EVPYALKQQLMVGGRLVIPLGRNDHFQHLVRLTRVSETEFDEVDLAPVRFVPLIAETY
                     H"
     gene            3348..>3892
                     /locus_tag="VFY27_11590"
     CDS             3348..>3892
                     /locus_tag="VFY27_11590"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX5766203.1"
                     /translation="MQLKRYRVALSGLVILMLLAGCSPPSAEAPQPTAAANEGTGSPT
                     GELQSLFDDEWAARLARDPLFASSMGVHRYDDRLPDESGEQHQRSLEQDLQFLERLRS
                     IDRTSLTNEEQLNYDLFEFILRSRTSLARYRPYLIPILSDAGFHMQVQRMYEAMPFES
                     VADYERYISRLRALSAYFRQN"
BASE COUNT          780 a         1183 c         1190 g          739 t
ORIGIN      
        1 acggcagaac gaccggaagc ggctctgtca gcgcggcgtt tgccgcgtcg agccagcgct
       61 gacccatgat tgtgttgttg agcccgttcg actcgatccg gcgcgcaaag tcctcatggc
      121 tggccggacc ctcggccatg agggtttcgc cgccgccgca gctggcgaac aagagaacgg
      181 tcgcgaggaa gagagagcgt aaaatccgac ccgggtagag gatgccgatt tccggagttg
      241 agacggctgc aatcattcac gattggaacg ccggcagccg cattagtttc acgagcacga
      301 ttcagcagct cacctggtgc tgcaggatca ctgatccaga gacaattcca atcgtcggcc
      361 gtctcgcgag atgttactgt ggctcggggg aagcgccgta ggaaaggatt ttcatggcag
      421 ccgactctca agtatcgcag ttgccgcctg cccgcatcgg catctctggc tggcgttacg
      481 cgggatggcg cggcaagttt tatcccgaag gcctgccgca aaagaacgaa ctcgcctacg
      541 ctgccggccg cttcaatacc atcgaactga acggcagctt ttattccttg cagcggcccg
      601 agagttacgc acactggtat cggcaagtgc cggacaactt cgtgttcgcg atcaagggat
      661 cacgcttcat aacgcacatg ttgaggctgc gcgacgtcaa gaccgctctg gcaaatttct
      721 ttgcctcggg ccttttcgag ctggaagaaa agctgggacc tattctctgg cagcttccac
      781 cgaccttcca gtacgaccgg gagcgcctcg aggccttttt cgcgttactg ccgaagaccg
      841 gcgccgacgc atcaaggctt gcgcgcaagc acgacgagcg catcaaggcg cgggcaagaa
      901 tcaggacgga ccccaacgcc aggctccggt atgcgctaga ggtgcggcat aagagctttg
      961 tgaaacccga cttcatcaac ctcctgcggg aacacatgat cgccctggtc gttgcggata
     1021 ccgcaggaaa gtggccgtac atggaggatg tcaccgcgga ttttgtctat gtgagactgc
     1081 acggggacaa gaagatctat gagagtggct actcgggcgc ggtgctcgat gcctgggcga
     1141 aaaaagtgga cgcgtggcga gttggcgaag aggtagccga tgtgcacaaa gtgtccgcga
     1201 aaagaccgcc ccggaaggaa gcacgcgagg tgttcgtcta cttcgacaac gacatgaagg
     1261 tacatgcgcc tgcggacgcc gcgcggcttg ccaaaaggct gtcggcacgc ccggcttgac
     1321 ggtcgcaagg cgcatggatt acagttgatt gaccaatcaa ctcagaggca ggggcgtgtc
     1381 cgagacacgt gaccggatcg taatggccgc catggatctg ttctgggaga agggctacgg
     1441 ttcgaccagt atttcggaca tcctgaagag ggccggtgcc aacagcggca gcctgtatca
     1501 cttctttccc gcgaagcagg atgtattgag cgctgtcctc gacacctatc accaggggat
     1561 cggcccgatg ttactggatc ctgcctggga cggggtggac gacccgctcg accgtatctt
     1621 cgtcctgctg gacagatacc gccgcttcct catcgacaca gactacttct acggctgccc
     1681 catcggcagt cttgccctgg aactccacga gccggatccc cccattcgcg aacgcctcgc
     1741 tgccaatttc tccgcctgga cggctgccat agaagagtgc cttgacgcgg ccccggaccg
     1801 cctgccgccg ggcgtcgaca agaaggcact cgcgcaattc gtgctcacga cgatggaagg
     1861 cggcgtcatg caggccagga cgtaccgcag tcttgagtcg ttcgacgcat ccgtcgcgca
     1921 gttacggaac tatttcgagt acctggccgg cacagcggcc agctgagaaa gaacacccga
     1981 cgatgcgcgc cggcgcgggt gcaggggaag aaggaaccag ccggtaaaag ccaataacaa
     2041 ggagaataac gatggaagat gcaatgggac agatcaacta cctggccgtg attgcggctg
     2101 ccgtgtcagc attcctgctc ggtggcctgt ggtactcggc attgttcggc aaggcgtgga
     2161 tggcggcgag cggtgtgacc aaggagcgtg cagctgaggc caaccaggcg aaagtcttcg
     2221 gagtcgcatt catctggtcg ctgctcgggg catatgtgtt cgcgatgttc cttggccccg
     2281 cgcccgcctt cggcttcgcc accgcagcag ggtttgcggc aggattattc tgggttgcgg
     2341 gttctttcgc catcaattac cagttcgagc agcggcctgc ccgactgctc ctgatcaacg
     2401 gcggatatca caccctccag tacacgttgt acgggtcgat cctgggtctg tggcactgac
     2461 ggatcagtga taggtttccg cgatcaacgg gacaaagcgc accggggcca ggtccacctc
     2521 gtcgaattcc gtctcggaaa cccgggttag tcgaaccagg tgctggaagt ggtcgttgcg
     2581 ccccagcggg atgacgagcc tgccgcctac catcagttgt tgcttcagcg cgtaagggac
     2641 ctccggggtg cctgctgcca cgacaatggc gtcgaagggc gccctgtcgg ggcagcccct
     2701 ggttccatcg ccgtaacgca tctcgacgtt gtcgtagccg agcgctgcca gcgtctcctt
     2761 tgcggattgc gccagcttct cgattctttc tatcgaacag actcgtcccg cgatctgccc
     2821 cagcaccgcc gcggcatatc ccgagccccc gcctacctcc agtacgttct ccccgccttt
     2881 aaggtccagt gcctcgatca tgagcgcgac aacgtgcggc tgggatatcg tttgcccggc
     2941 gccgatcggc agcggcgagt cctggtacgc cagcttccgc atgtgctccg gaacgaatgc
     3001 ctcccgcggc accgcccgca ttgcgtccag taccttggga gagcgtacgc cacgcgcggc
     3061 aatctgggtg gcgaccattg cgtgtcgccg gtcggagaga tcgtccgggg cgtctgggta
     3121 caacaccact tcaccaaatc agagcttgcg gcaacctttg tacagcaaga tacgtgcccg
     3181 gacgggggct ttgcccgcgc aaagcggaac cggtaacggc caggtaagcc ttacacgcgt
     3241 tgtaagtcct acattactcc tttgagtaag tgccagcttg cagaggtatc cgctgctgga
     3301 ggaccattgg ccgcggcaca aacggtcgga acgacggggg agaaaaaatg cagttgaagc
     3361 ggtatcgggt tgcgctctcg ggcctggtca ttctgatgct gttggccggc tgctcgccgc
     3421 cgtcagcaga agcaccgcag ccgacagcgg cagcgaatga aggcaccggc agccccaccg
     3481 gggaattgca gtccctgttc gacgacgaat gggcggcccg gctggcaagg gacccgctgt
     3541 tcgcaagctc gatgggagtc cataggtacg acgaccggct ccccgacgaa tccggggagc
     3601 agcatcagcg gtctctcgag caggacctcc agtttctcga gcgcttgcgc agcatcgacc
     3661 gaacgtcgct gacgaacgag gagcaactca actacgacct gttcgaattc atccttcgaa
     3721 gccgcacaag cctcgcccga tatcgcccgt acctcattcc catcctgagc gacgccggct
     3781 ttcacatgca ggtgcagcgc atgtacgaag cgatgccgtt cgaatcggtc gccgactacg
     3841 agcgctacat ctcccggctg cgggcgcttt ctgcctactt ccggcagaac at
//
LOCUS       DASUOM010000556         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_204653,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000556 DASUOM010000000
VERSION     DASUOM010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204653"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..873)
                     /locus_tag="VFY27_11595"
     CDS             complement(133..873)
                     /locus_tag="VFY27_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX5766204.1"
                     /translation="MIRRHVSLSLIIPLALTPQVHAQQVGSPPAEKQAEPWSGKAALG
                     YLATSGNTDSSSLNSGFALVYTTGRWAHSLEASAIKSSENDATSAEAYSVGWKTEFNM
                     TERDFLFGRVNWRKDRFSAYEQQLSESVGYGRRLIDTGVHFLNVELGAGARQADLLDG
                     TTEEDFILRGGINYQWKFTETAAFTQDINAESGPENVYTESVSAIKTKLIGNLGLVAS
                     YTIKNSSVVPPGSDNSDTFTALSLEYAF"
     gene            1028..1672
                     /gene="coq7"
                     /locus_tag="VFY27_11600"
     CDS             1028..1672
                     /gene="coq7"
                     /locus_tag="VFY27_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX5766205.1"
                     /translation="MHERRLSPTDRLFSGIDTVLRGIAGPKARSRRPCPADGIPDSQL
                     TPEQRRHAAGLMRVNHAGEVAAQGLYQGHAAVARSHSIAHHMREAAAEELDHLGWCEQ
                     RLEELGDVPSRLRPLWYAGSFAIGVASGALGDRWSLGFVEETERQVAEHLEGHLDRLP
                     PSDARSRAIVERMRIEEQQHGARAKAAGAESLPRLVHRLMRLIAKVMTRTSYRI"
     gene            1848..2501
                     /gene="crp"
                     /locus_tag="VFY27_11605"
     CDS             1848..2501
                     /gene="crp"
                     /locus_tag="VFY27_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEX5766206.1"
                     /translation="MQTTAKTLSDVPAINRFLSYCRVRTVPSKTVMIHAGDLPDVLYY
                     IVDGSVEVMIEDEDGNEMVLAYLNKGQFFGEMGLFYEQPTRSAWVRTRSECEIAEMTY
                     PRFRQIASESPGLVFELATQLATRLDRTNRKLGDLAFVDVTGRVAHAIMDLCNEPDAM
                     THPDGMQIKVSRQELSRLVGCSREMAGRVLKVLEEQGLVSASGKTIVVYGARPNRKI"
     gene            complement(2621..3304)
                     /gene="rpe"
                     /locus_tag="VFY27_11610"
     CDS             complement(2621..3304)
                     /gene="rpe"
                     /locus_tag="VFY27_11610"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5766207.1"
                     /translation="MEPLIAPSILSADFARLGEDVTAVLRAGADIVHFDVMDNHYVPN
                     LTIGPMLCKALRNYGIDAPIDVHLMVEPVDRIIPDFAEAGATYISFHPEATRHVDRTL
                     GLIRDSGCKAGLVFNPATPLDWLDYVLDKVDMVLIMSVNPGFGGQQFIESALQKLSRV
                     RKAIDDSGLPVRLEIDGGVKTDNIGKIAAAGADTFVAGSAIFGSDDYAATISAMRSEI
                     EKAGGGGAW"
     gene            complement(3346..>3990)
                     /gene="djlA"
                     /locus_tag="VFY27_11615"
     CDS             complement(3346..>3990)
                     /gene="djlA"
                     /locus_tag="VFY27_11615"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEX5766208.1"
                     /translation="QVPETFVRGLFLTMGFLAKADGRVAEEEIRAARVLMHRLGLGPS
                     EVREAIDWFEEGKHPSFPLHDVLRQVRRDTVRHPDLKLQFVRLLLEVALSKNSLHRRE
                     RAALWTICSELDVSRVDLAQLEAILRAQKGFRRSSAGGSDAERLTSAYAILGVNRFSS
                     NEDIKKAYRRLMNKNHPDKLATAGSDAALTTAAARRTREIRGAYEMLKARRSIR"
BASE COUNT          793 a         1204 c         1196 g          797 t
ORIGIN      
        1 agcaatgcaa acagcggctt agcggtcccg gcagcggcac ggccggggcg aacaaccggt
       61 taagtcttta ccatcagtgg cttatttgcg gctgtaagga agtcttgctg acgcgagcgg
      121 tatcgcctgc ggtcagaacg cgtactcgag cgacagggcg gtaaaggtat cgctattatc
      181 actccccggg ggaaccacgc tgctgttctt gatggtatag gacgccacca ggccgaggtt
      241 ccctatcagc ttggtcttta ttgcagagac ggactcggta tagacgttct cggggccaga
      301 ctcggcgttt atgtcctggg tgaaggcagc cgtttcagtg aacttccact ggtagttgat
      361 cccgccgcgc aggatgaagt cctcctcggt agtgccgtca aggagatccg cctggcgcgc
      421 accggcgccg agctccacgt tcaggaagtg cacgcccgtg tctatgagcc gtcggccgta
      481 gccgacagac tcggacagct gctgctcata ggcgctgaac cggtccttcc gccagtttac
      541 ccggccgaac aggaagtcgc gctccgtcat gttgaactct gtcttccagc caaccgagta
      601 agcttcggcg ctggtagcgt cgttctcgga agatttgatt gcgctcgctt ccagcgaatg
      661 cgcccagcgt ccggtcgtat agacaagcgc aaagcccgag ttcaggctcg agctgtccgt
      721 gttgccactg gtcgcgagat agccgagtgc cgccttgccc gaccacggct cggcctgctt
      781 ttccgcagga ggactgccaa cctgctgtgc atgaacctgc ggcgtcagtg ccaggggaat
      841 tatcaatgac aaggatacat gacgcctaat catgcgttgg ctccttcggc gtttcgtttg
      901 tggtaatcgg cgctaccgga gcaaggaatc tcgacgactg catccttggg acccccggcg
      961 cacgggacgc gcagtatatc gctgctcgca ccccgcgcga tacccgattc cgccttataa
     1021 tcccgccatg catgaacgcc gcctgtcacc gacggaccgc ctgttctccg gcatcgatac
     1081 tgttttgcgc ggtatcgcag gaccgaaagc ccgctcacgc agaccctgtc cggccgatgg
     1141 gataccggat tcgcaactga cgccggagca gcgccggcac gcggccggtc tcatgcgggt
     1201 caaccatgcg ggtgaagtag cggcccaggg gctttaccag ggtcatgcgg cagtcgcgag
     1261 aagtcattca atagcccatc acatgcgtga agcggctgcg gaagaactcg atcacctggg
     1321 ctggtgcgaa caacgtctcg aagaactcgg cgatgtgcct agccggctca ggcctctctg
     1381 gtatgcaggc tcgtttgcca tcggagtcgc cagtggcgcg cttggtgacc gttggtcgct
     1441 cgggttcgtc gaggaaacgg aacgccaggt agccgaacac ctcgaaggac atctcgaccg
     1501 tttgccgccg tccgacgcga ggagccgcgc gatcgtcgaa cgaatgcgaa tcgaggagca
     1561 gcagcatggc gcccgggcaa aggcagcggg tgccgaaagc ctgccgcggc tcgtgcatcg
     1621 actgatgcgc ctcattgcga aggtgatgac gcgaacgtca taccgaatat gacgcgaatg
     1681 gtttaatatc aggtgtacct caagttgctg ccggatgggg gtccagcagt ctaaaaaaca
     1741 cttttccaat aagattacgg acttagcttg ctcttgtgag caggctgtat taaaagctgt
     1801 atcaaaagct gtattaaaag aatgggcagt gccatcgagg attcagaatg caaaccacag
     1861 ccaagacttt gagcgacgtt ccggccataa accggttcct gagctactgc cgcgtgcgca
     1921 ccgtgccgtc gaagacggtg atgatccacg ccggcgacct cccggacgtt ctctattaca
     1981 tcgttgatgg ctccgtcgaa gtcatgatcg aggatgaaga cggcaacgag atggtgcttg
     2041 cttacctgaa caagggacag ttcttcggcg agatgggact cttctacgag cagcctaccc
     2101 ggagtgcctg ggttcgaacg cggagcgagt gcgaaatcgc cgagatgacc tatccgcggt
     2161 tccggcagat cgcgagtgaa agtccggggc tggtgttcga actggccacg cagctcgcga
     2221 cgcggctgga tcgcaccaat cgcaaactcg gcgatctggc cttcgtggac gtcacgggcc
     2281 gcgtcgcgca cgccatcatg gatctctgca acgagccgga cgcgatgacg cacccggacg
     2341 gcatgcagat caaggtcagt cggcaggaac tcagccggct ggtaggctgt tcacgcgaga
     2401 tggccggacg cgttctcaag gtgctcgagg aacagggact ggtatcggcg agtggcaaga
     2461 ccattgtcgt ttacggcgcc aggccgaacc gcaaaatctg acagggcgtt caagtcgact
     2521 gcaggacttg acgcataacg aaatccagag ggaaccggcg ggaagaccga agtcggggtg
     2581 cgggctattt agcagacgtc atcgcactcg ccagcatggg ctaccacgct cctccgccgc
     2641 ctgctttttc aatctcggaa cgcatcgctg agattgtcgc cgcgtagtca tcgctgccga
     2701 aaatggcgga acccgcgacg aaagtatccg caccggctgc cgcgatcttt ccgatattgt
     2761 ccgttttgac tcccccgtct atttcaagcc gcaccggtaa gccgctgtca tcgatcgctt
     2821 ttctcacgcg gctgagcttc tgcagtgccg attcaatgaa ctgctggccg ccgaaaccgg
     2881 gattgaccga catgatcagc accatgtcca ccttgtcgag cacgtagtcg agccagtcga
     2941 gcggcgtcgc cgggttgaac accaggccgg ccttgcaacc gctgtcgcgg atcaagccga
     3001 gtgtgcggtc gacgtgccgc gtcgcctccg gatggaagct tatgtaggtg gctcctgctt
     3061 cggcgaaatc cggaatgatg cgatcgaccg gctccaccat caggtgcacg tctataggcg
     3121 cgtcgatacc atagtttcgc aacgccttgc aaagcatcgg accaatcgtc aggttcggca
     3181 cgtagtggtt gtccatcacg tcgaaatgta cgatgtcggc cccggctctc agcaccgcgg
     3241 tgacgtcctc tcccagccgt gcgaaatcgg cggaaaggat tgacggtgcg atcagtggtt
     3301 ccacggtgcc tcccagaatc ctgtctctgc catcgacatt cttgactagc ggatgctgcg
     3361 ccgcgccttg agcatttcat acgcgccgcg gatctccctg gtgcgcctgg ccgcggcagt
     3421 tgtcaaagcc gcatcgcttc ctgccgtagc cagcttgtca ggatggttct tgttcatcag
     3481 gcgtctgtaa gccttcttta tatcctcatt gctggagaaa cgattgacgc cgagaattgc
     3541 gtaggcgctc gtgaggcgtt ccgcatccga cccaccggcc gacgacctgc gaaagccttt
     3601 ttgcgctcgc aggattgcct cgagctgcgc taagtcgacg cggcttacgt cgagttcgct
     3661 gcagatcgtc cagagcgccg cccgctcacg ccggtgcagg gagtttttcg acagcgccac
     3721 ttccagcaga aggcgcacga actgaagctt caggtccgga tgacgcaccg tatcgcgcct
     3781 gacctgccgc aatacgtcgt gcagcgggaa cgacgggtgc ttcccctcct cgaaccagtc
     3841 gatcgcttcg cggacttccg aaggccccag gccaaggcgg tgcatcagta cccgtgcggc
     3901 tcgtatctcc tcctccgcga cacgcccgtc cgccttcgcg agaaagccca tggtcaggaa
     3961 aagcccgcgg acgaatgttt ccggaacctg
//
LOCUS       DASUOM010000557         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1037210,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000557 DASUOM010000000
VERSION     DASUOM010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037210"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..394
                     /locus_tag="VFY27_11620"
     CDS             191..394
                     /locus_tag="VFY27_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766209.1"
                     /translation="MLALALPIGAVLPYDGSSREGAGCYLVAGRDIFDYHESVECFAC
                     AQGTGGDADVISRIELQIAVMSR"
     gene            complement(391..1881)
                     /locus_tag="VFY27_11625"
     CDS             complement(391..1881)
                     /locus_tag="VFY27_11625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX5766210.1"
                     /translation="MPAVERLCARNLDKLDPEVRRPAFDMRTSGLGIVHLGVGAFMRS
                     HIAEYTDDALEAGREDWTIAGVSLRKPDVRDRLAPQDCLYILGSRDDTGERRRLIGSL
                     ESMHVAPENPQHLIGLLADERVRVVTLTVTEKGYCLDPGSGQLSLAHPDIRHDLENRD
                     RPRSTIGYLAAALQLRMQASVRPPTIVSCDNLPSNGARLRSAVLAFARERDPALASWI
                     ARTVSFPETMVDRIVPATTEADISLNAGACGLRDEAYVKTEPFRQWVIEDAFSNGRPP
                     WDAGGALFVADVAPFEAAKLKLLNGPHSAIAYLGYLAGFNFVHEAMGDPDFSTFISRM
                     MTHDISPVAPEPDEMDHEIYIKELLRRFGNSALEHRTWQIAMDGSQKLPQRILSTVRA
                     QLAQGGPVASLGVVVAAWMRYALGRDEQGGKIDVSDPLAGRFAAIAADKHASPADIAG
                     RFLEITEIFGDDLPQDRRFRSTVVESLRQLFEYGALETVRRFVRLG"
BASE COUNT          399 a          660 c          648 g          397 t
ORIGIN      
        1 ggaaaaaaca attacggaaa gcgaccgcga agacccgtcc gataccattg tgaaagcggt
       61 aacgcagctc ttcgaggagt tcccgcgcta gcccgcccgg tgaattctca gagcgtcata
      121 ccgaactgtt cgtgattcgg ctcgggcaga ttggcttgct gtgaggttgt gcacgtgctc
      181 cccagccgca atgctcgcgc tcgccttgcc tatcggggcc gtacttcctt acgatggctc
      241 cagtcgcgag ggcgcgggtt gctaccttgt ggccggaagg gatattttcg attatcacga
      301 gtccgtcgaa tgtttcgcct gcgcgcaagg cacgggtggc gacgccgacg tcatctccag
      361 gatcgagctt caaatcgcag tcatgagccg ctagcccagg cggacgaagc gcctgacggt
      421 ctccagcgct ccgtattcga acagctgccg caggctttcc accaccgtac tgcgaaaccg
      481 ccgatcctgc ggaagatcgt cgccgaagat ttccgtgatc tccaggaacc tgcctgcaat
      541 gtcggcggga ctcgcgtgct tgtcggcagc aatcgcggcg aacctgcctg ccagcggatc
      601 gcttacgtcg attttccctc cctgctcgtc gcggccgagc gcgtaacgca tccaggctgc
      661 cacgaccacg ccgaggcttg ccaccggccc gccctgcgcc agctgcgccc gcaccgtcga
      721 gagaatccgt tgcggcagct tctgcgaacc atccatcgcg atctgccagg ttcgatgctc
      781 gagggcgctg ttaccgaagc gtcgcaacaa ttccttgatg taaatctcgt gatccatttc
      841 atcaggttcg ggcgcgacgg gcgatatgtc gtgcgtcatc attcggctga tgaatgtgct
      901 gaagtcagga tcgcccatgg cttcatgtac gaaattgaac ccggcaaggt agccaagata
      961 cgcgatggcc gaatgaggac cattgaggag cttgagcttt gccgcctcga atggcgccac
     1021 gtccgcgacg aacagcgctc cgcctgcatc ccatggcggc ctgccgttgc tgaaagcatc
     1081 ttcgatgacc cactggcgaa aaggttcggt ctttacatag gcttcgtcgc ggaggccgca
     1141 agccccggca ttcagggaga tatccgcctc cgtcgtcgcc ggcactatgc gatcgaccat
     1201 cgtttccgga aagcttaccg tcctggcaat ccaggatgcg agcgcgggat ctcgttcgcg
     1261 cgcgaacgcg agtaccgccg acctgagacg agctccgttc gacggcaggt tgtcgcagga
     1321 cacgatcgtc ggcggccgga ctgaggcctg catacgcaac tgcaacgcag ccgcgagata
     1381 gccgatcgtg ctgcgcggcc gatcccggtt ttcaaggtcg tgccggatat cggggtgagc
     1441 aagcgacagt tgaccgctgc cgggatcgag acagtaacct ttttcggtaa cggtgagcgt
     1501 cacgacgcgt acgcgttcgt ccgcgagcaa gccgatcagg tgttgtggat tttccggcgc
     1561 aacgtgcatc gactcgagcg agccgatcag cctgcgccgc tcgccggtgt catcgcggct
     1621 cccgagaatg tacaggcagt cctgcggagc cagccggtcg cgcacatcgg gtttcctgag
     1681 gcttaccccg gcaatggtcc agtcttcgcg cccggcctcc agcgcatcgt cggtgtactc
     1741 cgcgatgtgc gagcgcatga acgcgccgac gcccaggtgc acaatgccga gcccgctcgt
     1801 ccgcatgtca aacgcgggac ggcgaacttc ggggtccagt ttatccaggt tgcgagcgca
     1861 cagacgctcg acggcaggca tctcagttaa ccagtccggg tatccaaagg gagactgccg
     1921 gaatatacgc tacgacggcg agtgccacga gcagggcgag gtagaacggc cacgccacct
     1981 ccgagcggcg gcaggcaccc aacagtatcg gcgcggcaag cagctgacgt cgcgtcagcg
     2041 ggtatcacga tccagcttgt aggctttgcg cggcagggta taagcgaggt cggctgcgac
     2101 ttcg
//
LOCUS       DASUOM010000558         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1160326,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000558 DASUOM010000000
VERSION     DASUOM010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160326"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFY27_11630"
     CDS             complement(<1..230)
                     /locus_tag="VFY27_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5766211.1"
                     /translation="MQDVTIMFTSGPMRERRFEFEVGRVVIGRLPGGGGLELKGADTS
                     VSREHAVLEEADGDIELCNLSPNGTTVNGKLVL"
     gene            complement(244..1131)
                     /locus_tag="VFY27_11635"
     CDS             complement(244..1131)
                     /locus_tag="VFY27_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5766212.1"
                     /translation="MSTTQLIAYVAAFLSFALVVFAVRQAVSAFFVRYERLWDFETRH
                     SDSKAGALINLLKKVMVLEGRKIDSIDRMYSKLEIQVVRAASDMTAEEYLGQSLLEGL
                     LVLLVASAVCLFLFGPASLLIPVVVALAWAFFVRPSLVEGDAEKRSRAVYRRIPYTLD
                     LAVLVLQAGGTLREALEVSAARDDPLAEELRAALKEMDSGATQPAALKNMSNRVGLES
                     LDSIVMAIIRGLETGAPMAQTLAVQAELFRERRLQELEKLAVEAPTKMTFPNMLVMLS
                     VLVIILGPVLVKISKSGLL"
     gene            complement(1142..1993)
                     /locus_tag="VFY27_11640"
     CDS             complement(1142..1993)
                     /locus_tag="VFY27_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5766213.1"
                     /translation="MDLPIAYALAFLSAVLVAYLGLTAFYPPLRARVESDYLEVDRTL
                     KSIFYTAYDARLFVALKYGGTIVAFLVGMFLFNSFVFGAFMAAIVFPIPGMLLNRIVN
                     GRRDKLDHQTSDVMTALSATVKSGMTLEESITEIAANMRPPVSQEFALIKQRIDSGQS
                     IVSALRAADDRLQAPRLSLILQSIIVSQERGGRLSALMERLSEAMREIERVEERIKTE
                     TSGLRLSARIMVFLPFVIAGFLYIAEPAQVTMLFTTPIGNIILVVAIAMDIAAFRMMK
                     KIVDLDV"
     gene            complement(1993..>3260)
                     /locus_tag="VFY27_11645"
     CDS             complement(1993..>3260)
                     /locus_tag="VFY27_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX5766214.1"
                     /translation="GLQWSVSTDQVARDKFVETVVYELGLGANPGEGQIADVLANFDP
                     IFERNKRVLGKNQMLFIARESIRSDIEALVFGLGPLEDLLNLPDITEIMVVGKNRIFI
                     EKGGGLEATGRSFPSEDGLNVAVNRMVRPIGRTVNRSEPIVDARLEDGSRVHIVIPPV
                     AIKGTSVTIRRFRDTPFSIDDLVKFGTFGPRAVTFLRGCILARKNIVISGGTGSGKTT
                     LLNVLGAQIPFDQRLVVIEDSAELQLPQPNLVNLEARKANLEGEGEISIKDLVRASLR
                     MRPDRIVVGECRGGEALDMLQAMNTGHDGSMTTGHANSPREMIQRLQVMVMEAGGAMP
                     VSAIRQQIAAAVDLIVQIQRQRDRVRRVTHISEVVGFDQDTEEVVVEDIFALAEGRTG
                     PPELRFTGYLPSFIEPLLRHGGIKLEELF"
BASE COUNT          644 a         1053 c          946 g          617 t
ORIGIN      
        1 agcaccagct tgccgttgac ggtcgtgccg ttgggactga gattgcacag ttctatatca
       61 ccgtccgctt cctcgagtac cgcgtgctct cgcgataccg atgtgtccgc gcccttcagc
      121 tccagcccgc cccctcccgg caggcggcca atgacgacac gtcccacttc gaattcgaac
      181 ctgcgctcgc gcatcggtcc gctggtaaac attatggtca cgtcctgcat tttcagcgct
      241 tcctcataac aggccggact tgctgatctt gaccagaacc gggccaagaa tgatgacgag
      301 cacggacagc atcaccagca tattgggaaa tgtcatcttg gtgggcgcct cgaccgcgag
      361 cttctccagc tcctggagcc tgcgctcccg gaacagttcc gcctgcaccg cgagcgtctg
      421 cgccatcggc gcccccgtct cgaggccgcg gatgatggcc atgacgatgc tgtccaggga
      481 ctcgagcccg acgcggttgc tcatgttctt cagcgcggcg ggctgcgtgg cacccgagtc
      541 catctctttt agcgccgcgc gcagctcctc cgccagtgga tcgtcgcgtg ccgcggaaac
      601 ctccagcgcc tctcgcagcg tgccgccggc ctggagtacc agcaccgcga gatcgagcgt
      661 gtaaggtatg cggcggtaaa ctgccctcga gcgcttctcc gcatcgccct cgaccagcga
      721 aggcctgacg aagaacgccc aggccagggc aaccacgacc ggaatcaaca gggaggccgg
      781 gccgaacagg aaaaggcaga cagcgctggc gaccagcaga acgagcaagc cctcgagcag
      841 cgattggccc aggtattcct ccgccgtcat gtcggaggcc gccctcacca cctggatctc
      901 cagcttggaa tacatccggt cgatcgaatc gatctttcgc ccttccagga ccatgacttt
      961 cttcaggagg tttatgagag cacccgcttt tgagtcgcta tgacgtgttt cgaagtccca
     1021 caacctctcg taccgcacga agaatgccga aaccgcctgc cgtaccgcga acacgacaag
     1081 ggcgaacgac aggaatgccg ctacgtacgc aatgagttgg gtggtcgaca tctggattcg
     1141 cctacacgtc gagatcgaca atcttcttca tcatgcgaaa cgccgcgatg tccattgcaa
     1201 tcgcgacgac caggatgatg ttgccgatcg gggtggtaaa gagcatcgtg acctgcgcag
     1261 gctccgcgat gtagaggaaa ccagcgataa cgaacggcag gaacaccatg atccttgcgg
     1321 aaagccgcaa cccggaagtt tcggttttga ttcgctcctc gacgcgttcg atctcccgca
     1381 tcgcctccga aaggcgctcc atcagggcgg agaggcgtcc acctcgttcc tggctgacga
     1441 tgatcgactg caggatcagg ctgagcctcg gcgcctgcaa ccggtcatcg gccgccctca
     1501 gcgccgatac gatcgactgg cctgaatcga tacgctgctt gatcaacgcg aattcctggg
     1561 agaccggggg ccgcatgttt gcggctatct ccgttatgga ctcctcgagc gtcatgccgc
     1621 tcttgacggt agcgctcagc gcagtcatca catcgctggt ctggtgatcg agtttgtccc
     1681 gcctgccgtt gacgatacga ttcagcagca ttcccggaat cgggaagacg atcgccgcca
     1741 tgaaggcgcc aaagacgaag ctgttgaaca ggaacatgcc gaccaggaag gcgacgatcg
     1801 tgccgccata cttcaacgct acgaagagcc tggcatcgta agctgtatag aagatgctct
     1861 tcagcgtccg gtctacttcg agataatcag actccacgcg cgcccgaaga ggcgggtaga
     1921 aagcagtcag cccgaggtag gcgaccagga ccgcagacag gaaagccagt gcgtaggcaa
     1981 tcggcaggtc catcagaaga gttcctccag cttgattccg ccatgccgca gcaacggctc
     2041 tatgaagctc gggaggtagc cggtgaacct cagttccggc ggccccgtac ggccttccgc
     2101 cagcgcgaat atgtcctcga cgacgacctc ctcggtatcc tgatcgaaac cgacaacttc
     2161 gctgatgtgc gtcacccgcc gcacgcggtc tctttgccgc tggatctgca cgatgaggtc
     2221 gacagccgcg gcgatctgct gccttatcgc ggaaaccggc atcgcgccgc ctgcttccat
     2281 caccatgacc tgcaggcgct ggatcatttc gcggggagag ttcgcgtgac ccgtggtcat
     2341 cgacccgtcg tggccggtgt tcattgcctg cagcatatcc agcgcctcgc cgccgcggca
     2401 ctcgccgacc acgatccggt ccggccgcat gcgcagcgaa gcgcgaacga ggtccttgat
     2461 cgagatctcg ccttcgcctt ccaggttcgc cttccttgct tcgaggttca cgaggttcgg
     2521 ctgcggcaac tggagttcgg cgctgtcctc tatgaccacg aggcgctggt caaaaggaat
     2581 ctgcgccccc agcacgttca acagcgtggt ctttccggat cccgtaccgc ccgaaatgac
     2641 gatgttcttg cgcgctagaa tgcagccgcg gagaaacgtc accgctctcg gaccgaaggt
     2701 cccgaacttg accaggtcat cgatggagaa cggcgtatcc cgaaaccggc gaatggtgac
     2761 ggaagtaccc ttgatcgcga ccggcggaat gacgatgtgc acgcgggagc cgtcttcgag
     2821 gcgcgcgtcg acgatcggtt cggagcggtt gacggtacga ccgatcggcc gaaccatcct
     2881 gttgacggcc acgttcaacc cgtcttcgga gggaaagctg cggccggttg cctcgaggcc
     2941 gccgcccttc tcaatgaaga tgcgattctt gccgacgacc atgatttccg tgatatcggg
     3001 caggttcaaa aggtcctcga gcggccccag tccgaaaacg agcgcctcga tgtccgagcg
     3061 aatggactcc ctggcgatga acagcatctg gttcttgccg aggacccgct tgtttcgctc
     3121 gaagatcggg tcgaagttcg cgagcacatc cgcgatctgc ccttccccgg gattcgcgcc
     3181 cagcccgagc tcgtaaacga cggtttcgac gaacttgtcg cgcgccacct ggtccgtgct
     3241 gacactccac tgcaggccct
//
LOCUS       DASUOM010000559         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_694046,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000559 DASUOM010000000
VERSION     DASUOM010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694046"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFY27_11650"
     CDS             complement(<1..191)
                     /locus_tag="VFY27_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766215.1"
                     /translation="MPVLVGRAIYCGTVALLPMFISLSATAQYDADGNEADVVEEIVV
                     TGSRIKRRDFSSPSPIATI"
     gene            345..1757
                     /locus_tag="VFY27_11655"
     CDS             345..1757
                     /locus_tag="VFY27_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5766216.1"
                     /translation="MSKQEKWTRREFSKATLAATAVAATNGGVAIAATAERPNILVFV
                     ADDAGARHFGCYGNRAVRTPNIDRLSSEGITADNAMLTTSQCSPSRISILTGKYPHAT
                     GAEDLHMPMPERHTPLQAYLREAGYFTGHMQKVHEGSHSDSKFHWYDKGLDRLPAFLD
                     AAGDRPFFLWVAFDDPHRPYQDDTIAEPHGPERVEVPPYMVDTRETRADLARYYDEIG
                     RMDETVGRHLRILEERRLEQDTLVVFLSDNGAPFPREKGTVYDGGVRTPLIFRRPGTV
                     PRGLRHDGLMSVIDLAPTFLSIAGIDVPDDMQGHDISPGLRDPRAWRRDAAFSERNWH
                     DCDEHIRSIRTAGYRLIHNAYIELPFGSPADVTASPSWLALYEGKKRGQLTETQHLLF
                     ESPRPEIEFYDARQDEWQVENLAARPEYQQEIAAHYAMLKEWMTATLDFPPERRRRAG
                     YVDRVTGVLFSLDVPPMFNE"
     gene            1825..>2297
                     /locus_tag="VFY27_11660"
     CDS             1825..>2297
                     /locus_tag="VFY27_11660"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX5766217.1"
                     /translation="MAHIQPASITVDDTRSVSALLQLPADAQACLVLAHGAGAGMHHA
                     FLQTLATELDGRSIATLRYQFPYMERGSRRPDPPPLAHATVRAAVNAAARLAPGIPLF
                     AGGKSFGGRMTSQAQAADLLPGVRGIIFVGFPLHPAGRPSTTRFDHLHDVTTPML"
BASE COUNT          463 a          752 c          678 g          404 t
ORIGIN      
        1 tctatcgtcg cgatgggact gggagaactg aagtccctgc gtttgatgcg tgaacccgtg
       61 acgacgattt cttcaacgac gtccgcctca ttaccgtccg catcgtactg ggcagtggca
      121 gagagcgaaa tgaacattgg cagcaaggcg acggtgccac aatagatcgc tcggccaacc
      181 aagacaggca taaggattct ccccgatccc gccagctctt tttccagcca atcttacgct
      241 gttctcctgc gaagatgtca cccatcgggc cgccgcacga gggctttaag cgaaacgaac
      301 gtataattct ccggccaaga cttctgctta cagtgagccg gggtatgtcg aaacaggaaa
      361 agtggacacg ccgggagttc agcaaggcaa cgctggcagc gacagcggtg gctgcaacga
      421 acggaggagt cgcaatcgct gccaccgccg agcggccgaa catcctggtg ttcgtcgccg
      481 acgacgccgg ggcgcgccac tttggctgct atgggaatcg cgccgtacgc acgccgaaca
      541 tcgaccgttt gtccagcgag ggcataaccg cagataacgc gatgctgacg acctcgcaat
      601 gcagcccgtc gcgcatcagc atcctcaccg gcaagtatcc gcacgcgacc ggggctgaag
      661 acctgcacat gccgatgccg gagcggcata caccgctgca ggcgtacctg cgtgaggccg
      721 gctatttcac gggccacatg cagaaggtgc atgaagggtc gcactcggac agcaagttcc
      781 actggtacga caaagggctc gaccggcttc ctgcctttct cgatgccgcg ggcgacaggc
      841 cgttcttcct gtgggtggcg ttcgacgatc cgcatcgccc ttaccaggat gacactatcg
      901 ccgaaccgca cggtccggaa cgggttgaag tgccgccgta tatggtagac acccgcgaga
      961 cgcgtgccga cctcgcgcgc tactacgacg agatcgggcg catggacgag acagtcggac
     1021 ggcacctcag gatccttgaa gagcgccgcc tcgagcagga cacgctggtc gttttcctga
     1081 gcgacaacgg tgcgccgttt ccgcgcgaga agggcactgt ttacgatggg ggtgtcagga
     1141 cgccgctcat ctttcgcaga cccggcacgg tcccgcgagg cctgcgccac gacgggctga
     1201 tgagcgtgat cgatcttgcg ccgacgttcc tgtcgatcgc gggcatcgac gttcccgacg
     1261 acatgcaggg ccacgatatc tctcccggcc tgcgcgatcc tcgcgcctgg cgcagggatg
     1321 cagcattcag cgagcgcaac tggcacgact gcgatgagca tatccgcagc atacgtaccg
     1381 caggttatcg cctcatccac aacgcgtaca tcgaactgcc gttcggtagc cctgccgatg
     1441 tgacagcaag tccttcatgg ctcgcgcttt acgagggcaa gaagcgcggc caactgacgg
     1501 aaacgcagca cctgttgttc gagtcaccgc gcccggagat cgagttctac gacgcgcgcc
     1561 aagacgaatg gcaggtcgag aacctggcgg cgcggccgga ataccagcag gaaatcgcag
     1621 cgcattacgc gatgctgaaa gagtggatga ctgcgaccct ggatttccca ccggagcgcc
     1681 ggcgcagggc aggttatgtc gatcgcgtaa cgggcgtcct gttctccctc gacgtcccgc
     1741 cgatgttcaa tgaataaacg caattgagga cccggcgacg gacttgcggt caaaatacgc
     1801 tcttctgcaa tgcaaagttc atcaatggct cacatacaac ccgcatccat caccgtcgac
     1861 gatacccgca gcgtctccgc gctcctgcaa ctgcccgctg acgcacaggc ctgcctcgta
     1921 ctcgcgcatg gcgccggtgc cggcatgcac catgcattcc tccaaacgct ggcaaccgaa
     1981 ctcgacggtc gcagtatcgc tacccttcgg taccagttcc cttacatgga gcgcggctcg
     2041 cgacgcccgg acccgccccc gctggcccac gccacggtac gcgcggccgt caatgcagcc
     2101 gcgaggctcg caccgggaat cccgctattc gccggcggca aatccttcgg tggccgcatg
     2161 acctcgcagg cacaggccgc ggacctgttg ccgggcgtcc gaggcataat cttcgtcggt
     2221 tttcctcttc acccggcggg caggccctcc acgacgcgct tcgatcacct tcacgacgtg
     2281 actacgccga tgctgtt
//
LOCUS       DASUOM010000560         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1762904,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000560 DASUOM010000000
VERSION     DASUOM010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762904"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFY27_11665"
     CDS             <1..626
                     /locus_tag="VFY27_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5766218.1"
                     /translation="TFATPCLQRPLEFGCDIVMHSVTKFLNGHSDMVGGIAVTGDEEL
                     AERLAFLQNSIGSVQGPFDSFLALRGLKTLHVRMQRHCSNAQAIAGWLEKDARVESVI
                     YPGLESHPQHRLAKSQMSAFGGIVTFFIKGGLEEARTFLERCEIFALAESLGGVESLV
                     DHPAIMTHASVPAEKRRALGISDKLIRLSVGIEDLADLIADLDHALG"
     gene            complement(637..1719)
                     /gene="prfA"
                     /locus_tag="VFY27_11670"
     CDS             complement(637..1719)
                     /gene="prfA"
                     /locus_tag="VFY27_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5766219.1"
                     /translation="MKESIRDKLESTRDRFQELSGLLSDPGVIGNQNQFRDLSREYSR
                     LEPVVNLFSQYERLQGEIAAAEEMAADPDREIRQMGREELETLNERLEELQLALQKAL
                     IPRDPYDESNVFLEIRAGTGGDEAAIFAGDLFRMYAKYAEQMRWQLEVLGEREGEHGG
                     YKEIVSRISGHGIYGRLKFESGAHRVQRVPATESQGRIHTSACTVAILPEPDELEEIE
                     IDVNDLRVDTYRASGAGGQHVNKTDSAVRLTHLPSGIVVECQDERSQHKNRARAMSLL
                     QAKLLDAELSRRQAEQAQQRKLLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLQEI
                     LGGDLDQVIDPLVNEYEADQLAALAH"
     gene            complement(1716..>2436)
                     /locus_tag="VFY27_11675"
     CDS             complement(1716..>2436)
                     /locus_tag="VFY27_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5766220.1"
                     /translation="KNLHGKGIGRMFVANRDILRAQNLASQYGGFALPLSELDGILPE
                     ADILISSTASPEPIINVEQMKKAVRARKRKPVFAVDLAVPRDLDPAISTLDDVYLYTI
                     DDLDKVVVEGQSNREAAVADANRILEEETRRYLDIERGKEVAPVITALRDHGDTLRQE
                     VLEQGRRRLDKGADTHEVLEYVTASLLKKLLHQPSVRLRDAGAASDTRFIEAVRELFG
                     LAETGDQPLANKDRRKDVTSK"
BASE COUNT          391 a          765 c          766 g          514 t
ORIGIN      
        1 acacttttgc cacgccctgc ctgcagcgtc ccctggaatt cggctgtgac atcgtgatgc
       61 attccgtgac gaagttcctg aacggtcact cggacatggt cggcggcatc gccgtcaccg
      121 gcgacgagga actcgccgaa cggctggcct tcctgcagaa ctccatcggt tctgttcagg
      181 ggccgttcga cagcttcctc gcgctgcggg gactcaaaac cctgcacgtg cggatgcagc
      241 gtcactgcag caacgcccaa gcaatcgccg ggtggctgga gaaggatgca cgcgtggaga
      301 gcgttatcta tcccggcctc gaatcgcacc cgcagcacag gttggcgaaa tcgcagatgt
      361 cggcctttgg cggcatcgtc acgttcttca tcaagggcgg gctcgaggag gcacgcacgt
      421 tcctcgaacg ctgcgaaatc ttcgcgctcg ccgagagcct ggggggtgtc gaaagcctcg
      481 tagaccatcc cgcaataatg acccacgcgt cggtaccggc cgaaaagcgg cgggcgctag
      541 gcatcagcga caagctgatc cgcctgtcgg tcggcatcga ggatcttgct gacctcatcg
      601 ccgacctcga ccacgccctg ggctgaaaag tttggttcaa tgcgccaggg cggcgagctg
      661 gtcggcttcg tactcgttga cgagcgggtc gatgacctgg tcgaggtcgc cgccgaggat
      721 ttcctgcagc ttgtaaagcg tcaggttgat gcggtgatcg gtgacgcggc cttgcgggaa
      781 attgtaagtg cggatccttt cggagcggtc ccccgagccg accagcagtt ttctttgctg
      841 cgcctgttcc gcctgccgtc tcgacagttc ggcgtccagc agttttgcct gcagcaggga
      901 catggcgcgg gcacggttct tgtgctgcga gcgctcatcc tggcattcga cgacgatacc
      961 gctcggcaga tgggtgagcc tgacggcgga atcggtcttg ttgacgtgct gtccgccggc
     1021 gccggaggcg cgataggtgt cgacgcgcag gtcgttcacg tcgatctcta tctcctcgag
     1081 ttcgtcaggc tccggaagga tcgcaacagt gcaggcggag gtgtgaatgc gcccctgcga
     1141 ttccgtcgcc ggaacgcgtt gcacgcgatg tgcgccggac tcgaacttga gcctgccgta
     1201 aattccgtgt ccggagatgc ggctgacgat ttccttgtag ccgccgtgct cgccctcgcg
     1261 ttcacccagc acctccagtt gccagcgcat ctgttctgcg tacttcgcgt acatgcggaa
     1321 caggtcgccc gcgaagattg cggcttcatc tccgcccgta cccgcacgaa tttccaggaa
     1381 gacgttgctc tcgtcgtagg gatcgcgggg aatcagcgcc ttctgcagcg cgagctgcag
     1441 ttcctcgagc cgctcgttca gcgtctccag ttcctcgcgg cccatctggc ggatctcgcg
     1501 atcggggtcg gcggccatct cttccgccgc ggcgatttcg ccctgcagcc gctcgtactg
     1561 gctgaagagg ttcaccaccg gctcgaggcg cgagtattca cgggacaggt cgcggaactg
     1621 gttctggttg ccgatgacgc ccggatcgga aagcaggcct gacagttcct ggaaccggtc
     1681 ccgggtggac tcgagcttgt cccttatcga ctccttcatt tcgacgtcac gtccttgcgg
     1741 cggtccttgt tcgccagcgg ctggtcgccg gtttccgcga ggccgaagag ttctcgtaca
     1801 gcttcgatga agcgcgtatc ggacgcggcc cccgcatcac gcagccgcac gctgggctgg
     1861 tgcaacagct tcttgagcaa tgacgcggtc acgtactcga gcacctcgtg ggtgtcggcg
     1921 cccttgtcga gccgccgccg gccctgttca aggacttcct gccgcagggt gtccccgtgg
     1981 tcgcgcaggg cggtgatgac cggtgcgact tccttgcctc gttcgatgtc gaggtagcgg
     2041 cgggtttcct cttccaaaat ccggttcgca tcggcaactg ccgcttcccg attgctttgt
     2101 ccctcgacca cgaccttgtc gaggtcgtca atggtgtaga ggtaaacgtc atcgagcgtg
     2161 cttatcgccg gatcgagatc ccgcggcact gcaagatcga ccgcgaacac aggttttctc
     2221 ttgcgcgcgc gcacggcttt cttcatctgc tcgacgttga tgatcggttc cgggctcgca
     2281 gtggagctga tcaggatgtc ggcctcgggc agaatcccgt ccagttcaga cagcggcagc
     2341 gcaaagccgc catactggct ggccaggttc tgtgcgcgca ggatgtcgcg gttcgcgacg
     2401 aacatgcgac cgatgccttt gccgtgcagg ttcttc
//
LOCUS       DASUOM010000561         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_797337,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000561 DASUOM010000000
VERSION     DASUOM010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797337"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFY27_11680"
     CDS             <1..973
                     /locus_tag="VFY27_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766221.1"
                     /translation="GRPAHAVLLTGSAGVGKRAAASWMAMQRLGLDAPALPTVQVVPV
                     EGDMPADERLAQHPDFRWLRPPEGKQAILIDQVRELVGELSLTSYEGGAKVVVIEPAN
                     MLTGNAANSLLKTLEEPQGDALLLLVADRMGSLPATILSRCQRITIATPPESVSLSWL
                     ERLRPGANWRAALRMAGNAPLAAIEALERLDVTDAMARDFTQLAERRVSPIDVAARWS
                     QYEPDFVLEWMGRQLQQCIYRTSGAAGESFEGSPAESVLNRIDRRKLFCYLDDINRVR
                     GQSAGSYNVQLTLESLLIDWAQGLKDSGNKFIRGGLLPIPDGKVNAQ"
     gene            970..1308
                     /locus_tag="VFY27_11685"
     CDS             970..1308
                     /locus_tag="VFY27_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5766222.1"
                     /translation="MTKPGLLTLTIKDKSALYLAYMPFVVNGGLFIPTNSSYRLGDEV
                     FMLLNLMGEEEKIPVAGTVIWMTPKGAQGKRTAGIGVQFSDQDQGNTQKKIENYLAGA
                     LGGDKPTHTM"
     gene            complement(1398..2444)
                     /locus_tag="VFY27_11690"
     CDS             complement(1398..2444)
                     /locus_tag="VFY27_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5766223.1"
                     /translation="MKDGPLDKELLKSFSPLDGLKRDNLAALAKKVQIRELSPQQILF
                     KEGDTEKRTVYIVSGSLELIDQGQVQEIIEGGTDSARNPVSPMFPRRVTAKARNRVQY
                     ISIDSDLLDVMLTWDQTGSYEVSELKGDSSSGSDDWMTMLLQTKAFHKIPPANIQAIF
                     MRMQQINYKAGDVVLKQGAEGDYFYVLTRGKSLVTRETPLNKEGIKLAELSVGDTFGE
                     EALISDAKRNATVTMLTDGAVMRLGKEDFKRLLNEPMLDWVTPAEAEEIVKNGGHWLD
                     VRLPSEFENQHYDGAINVPLYFIRLKINTLDPKKKYVVCCDTGRRSSAGAYILSERGY
                     NVYVLKGGINSGQN"
BASE COUNT          506 a          763 c          784 g          542 t
ORIGIN      
        1 tggccggccc gcacatgccg tcctgctgac cggcagtgcc ggggtaggca agcgcgccgc
       61 cgcatcctgg atggcgatgc aacggctggg cctcgacgca ccggcgttgc caaccgtgca
      121 ggtggttccg gtggaaggtg acatgcctgc ggatgagcgc ctcgcgcagc atccggactt
      181 tcgctggctc cggccgcctg aagggaagca ggcgatactc atcgaccagg tccgcgaact
      241 ggtcggcgaa ctgtccctga ccagttacga aggcggcgcc aaggttgtcg tgatcgagcc
      301 ggctaacatg ctgacaggca acgctgccaa cagcctgctg aagacgctgg aagaacccca
      361 gggagatgca ctgctgctgc tcgttgcgga tcgcatggga agcttgccgg ccacgatctt
      421 gagccgctgc cagcgaatca ccattgctac tccgcctgaa agcgtaagcc tttcgtggct
      481 ggagcgctta cgaccggggg cgaactggcg cgccgcgctg cgcatggcgg gtaatgcgcc
      541 gcttgcggca attgaagcgc tcgaacggct ggatgtaacg gatgcgatgg cccgggattt
      601 cactcagctg gcggagcgtc gggtttcgcc gatagacgtg gcagctcgct ggagccagta
      661 tgagccggat ttcgtgctcg agtggatggg acgccagctg caacagtgca tctacaggac
      721 aagcggagct gctggagaaa gcttcgaggg aagtccggca gaatctgtgt tgaaccgcat
      781 agacaggcga aaattgttct gctatctaga cgacatcaac cgcgtcaggg gccaatccgc
      841 gggctcatac aacgttcagt tgactctgga gagcctgctg atcgactggg cacagggtct
      901 caaagacagc ggcaacaagt ttatccgggg cggcttgctg ccgattccgg acggtaaggt
      961 gaatgcgcaa tgacaaaacc cggtttattg acactgacca tcaaggacaa gagtgcgctg
     1021 tacctggcgt acatgccctt tgtcgtgaac ggcggccttt tcatacccac gaacagttcc
     1081 taccgcctcg gagacgaggt attcatgctc ctgaacctga tgggcgagga agagaagatc
     1141 ccggtggcag ggacagtgat ctggatgacg cccaagggcg cccaggggaa gaggacggca
     1201 ggcatcggcg tgcagttcag cgaccaggac cagggcaaca cgcagaagaa gatcgagaac
     1261 tacctggccg gcgcgctggg cggcgacaag cccacccaca cgatgtaatt ctgcgcagga
     1321 gcccgccatg cgggcgagat ggcaaatgtc acgcgttgtg gcgcgtgtca gttcacgagg
     1381 gggtcgaaac caccacgtca gttttgcccg gaattgatcc cgcccttcag cacgtaaacg
     1441 ttgtagccgc gctcgctgag aatgtaagcg cccgccgaac tgcgtctgcc agtgtcgcag
     1501 cagacgacgt atttcttctt cgggtccagc gtattgattt tcaggcggat gaagtagagc
     1561 gggacgttga tcgcgccatc gtagtgctgg ttttcgaatt cgctgggcag gcgtacgtcc
     1621 agccaatgcc cgccgttctt cacgatctcc tcggcttcgg caggcgtgac ccaatccagc
     1681 atcggctcgt tgagcaggcg cttgaagtct tccttgccca gtctcatgac ggccccgtcg
     1741 gtcagcatcg tgactgtggc gttgcgcttt gcgtccgata tcaaggcttc ttcaccgaac
     1801 gtgtcgccca ccgaaagctc ggccagcttg atgccttcct tgtttaacgg tgtctcgcgg
     1861 gtgacaaggc tcttgccgcg cgtgagcacg taaaagtaat cgccttcggc gccttgcttc
     1921 agcacaacat cgccagcctt gtagttgatc tgctgcatgc gcatgaagat ggcctggatg
     1981 ttcgccggcg gtatcttgtg gaacgccttc gtctgcaaca gcatcgtcat ccagtcgtcg
     2041 ctgccggagc ttgagtctcc cttgagctcg gacacttcgt aggagccggt ctggtcccat
     2101 gtgagcatga cgtcgagcaa atcactgtcg attgatatgt actgcacccg gttgcgcgct
     2161 ttcgcagtga cgcgccgcgg aaacattggc gatactggat tcctggcgct gtcagtacct
     2221 ccctcgatga tttcctgaac ctgtccctgg tcgatcagct ccagcgaacc ggacacgatg
     2281 taaacggtcc gcttctcggt atctccctcc ttgaagagga tctgttgcgg cgacaattcc
     2341 cgtatctgca ccttcttcgc cagcgcagcg agattatccc gcttcagtcc atccaatgga
     2401 gaaaagctct tgagcagttc cttatccagc ggcccgtcct tcatttttca tcccgttcac
     2461 tcgattgtcg tgtagcgtaa gtacgcacgt tccccggagg atcgctcgtt gccggtgcac
     2521 actgcccccg aacaccggcg ccgtagcgcg gcatttgctg gtttgcccgg gctaagccgt
     2581 tattctacct tcaat
//
LOCUS       DASUOM010000562         5563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1179351,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000562 DASUOM010000000
VERSION     DASUOM010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179351"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..553
                     /locus_tag="VFY27_11695"
     CDS             230..553
                     /locus_tag="VFY27_11695"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5766224.1"
                     /translation="MRLAATRLRGRKVINYDNEDLGTIEDFMLDTATGRFGYAVLSCA
                     AIAGKQKLFPVPLHALTVDTDGGRFMLNADRETLSYAPGFGPGDWPDTGDPRWKSGVN
                     RFYSF"
     gene            625..1245
                     /locus_tag="VFY27_11700"
     CDS             625..1245
                     /locus_tag="VFY27_11700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5766225.1"
                     /translation="MTFKLTELPYDLDALEPHVSARTLELHYGKHHKGYVDKLNQNAG
                     GTRYEDMALEEVIQQSHKNDDTPVYNNASQAWNHTFFWDSMSPGEASRPSGSLSDMID
                     DQFGGLDEFRKQFKESAVGRFGSGWTWLVADEDALAIVSTSNADSPLITPTAPLITLD
                     VWEHAYYLDYQNARDKFVDAFLGHLINWDFAEDNLDAYTTRRQLDV"
     gene            complement(1271..1483)
                     /locus_tag="VFY27_11705"
     CDS             complement(1271..1483)
                     /locus_tag="VFY27_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766226.1"
                     /translation="MNDTEKAHLKEALHKMNNALNSISMQAELAKMYAEQDNSEMVGE
                     ALGIIMSQCRQCSAVTQSAHSLLQGD"
     gene            complement(1494..2807)
                     /locus_tag="VFY27_11710"
     CDS             complement(1494..2807)
                     /locus_tag="VFY27_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5766227.1"
                     /translation="MARILLVDDDTDFTSATQTLLRAEEHDVSTVGSVAEASGVLRRE
                     NFDMLFVDLSLPDGSGLELIKDDGPKAVIITGHPSIETAIRAVRGRVVDYLVKPLDKN
                     QLIKSIESAALDKPTRRIPSIEAIRPANMIGESAAMKSLMKSIAEYGPTDVTILITGE
                     SGTGKDLVAQALHNACHSDAKFVPVNCGAIPRELVASELFGHEKGSFTGAGAKRVGIF
                     ERAKNGTVFLDEIGELPLDQQVALLRVLETGSMVRVGGEKEIPMTARIIAATNKDLEK
                     EVSEGKFREDLFFRLMVLPIHVAPLREREGDIPLLANYFLEMYSKEHGTPSRFSPRVL
                     SKLETYHWPGNVRELKHTVLRAAILNRGKDKVDTLPDNFDRPPKWSEDSQGLRAGMSI
                     RDVEKTLIEKTLEHFDGNKKLTAEALGISLKTLYNRLCEYEEDRV"
     gene            complement(3010..3963)
                     /locus_tag="VFY27_11715"
     CDS             complement(3010..3963)
                     /locus_tag="VFY27_11715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5766228.1"
                     /translation="MQILIVEDNDVVAQGLSRALSMLDSRISVYRAADLQSAQKLLQD
                     GPRIDVALVDLGLPDATGIEAPTKLKIFSPELVIVVITGDSSSETALSLIRLGIQDYI
                     PKSEATPNRIFRTIRLARERYEREQRLKRIACIDQLTGSLNRRGLLSAIKESNDVAVR
                     LNIPSALMTIDIDHFKEINDTYGHPIGDLILRQGCRRIAHCIRENDVVGRAGGDEFWV
                     VLNGFTKPENIPAIANKMLKSFAAPFRVNSEINASISIGVALMPDHAGTTQEWMLKSD
                     LALYEAKRDGRNRWAMYHDGLQPVQGSEPVRPSGLVKLQSV"
     gene            4421..4630
                     /locus_tag="VFY27_11720"
     CDS             4421..4630
                     /locus_tag="VFY27_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766229.1"
                     /translation="MDVINNEWGFPMTMLALISVLAIFGTALWFILSAMDTNKKFRWF
                     QIVGRRERQERTKRTSEWEQQKRPR"
     gene            4761..>5563
                     /locus_tag="VFY27_11725"
     CDS             4761..>5563
                     /locus_tag="VFY27_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5766230.1"
                     /translation="MSLRSHLLLLTLAVLLPLAIFGVGATLWVADRERDAFEQGARQR
                     TLALITAVDTELGGHAGTLRALASSYNLEAGNLPAFHRQAMRLLQTQENWRSIILAMP
                     SGQRILDSSMPLDAALSVIDRTGFDEALRRKEPTIGNLSPEDSEYHFSVLMPVQSTGQ
                     VYILMALVDPQAVLSLLTPQRLPRDWVGTVLDSNNRVVARTFNSSATVGKPASESLQA
                     ALRQAAEGWFHGTTIEGADVYASFHQSSVTGWTVALGIPSAVVGATTSH"
BASE COUNT         1097 a         1635 c         1594 g         1237 t
ORIGIN      
        1 aactgcatgc tggcgtcgag ttgacacgcg cttggactgg cggtcgccgt gcaattagca
       61 agtccctggc ggacttgcga tctgcacggc ggccgaccac tctttttcca accacagaat
      121 gacggctaca gcgttgggaa ctgatatggc ggtcggaaca ggtacgcggt tgcggcaggc
      181 cagcgccaat ggccgcgccg ccttgatgac aacagcggga ggcgcgacca tgcgtcttgc
      241 cgccacacgg ctgcggggtc gcaaagtcat caactatgac aatgaagatc tcggtacgat
      301 cgaagatttc atgctggata cggccaccgg gcggtttggc tatgccgtac tctcctgtgc
      361 cgccatagca ggcaaacaaa agctctttcc tgtacccttg catgcactga cggttgatac
      421 cgacggtggg cgttttatgc tgaacgccga tcgggaaacg ctaagctacg ctcctggatt
      481 cggcccaggc gactggccgg acacgggcga tccacggtgg aaatccggcg tcaacaggtt
      541 ttacagcttc tgacgactcc accgtcccgt cgctactccc gatatgcctg atccctcccc
      601 tgacgagttt catggagtct gtcgatgaca ttcaaactta ccgaattgcc ttatgatctt
      661 gacgcgctcg agccgcacgt gtcggcgagg acactggagt tacactacgg caagcaccac
      721 aaaggctatg tggacaaact gaaccagaat gccggcggaa cccgttacga ggatatggcg
      781 ctcgaggaag ttattcagca atcgcacaag aacgacgaca ccccggtgta caacaatgca
      841 tcgcaggcgt ggaaccatac ctttttctgg gacagcatga gtccgggaga agcaagccgc
      901 cccagcggtt cgctgtccga catgatcgac gaccagttcg gcgggctgga cgagttccgc
      961 aagcagttca aggaatcggc cgtgggcagg tttggcagcg gttggacctg gctggtagcg
     1021 gacgaggacg ctcttgccat cgtttcgaca tcgaatgccg attctccgct cataacgccc
     1081 actgctccgt tgataaccct ggacgtctgg gaacacgcct attacctcga ctaccagaac
     1141 gcccgtgaca agtttgtcga tgcgttcctc gggcacctga tcaactggga cttcgccgag
     1201 gacaatctcg acgcgtacac gacgcgacgt cagttggacg tttagcactc cctggggtgc
     1261 tgtggggcga tcagtctccc tgcaagaggc tatgagcgct ctgcgtaacg gcgctgcact
     1321 gccggcactg agacatgatg atgccgagcg cttcacctac catctcgctg ttgtcctgtt
     1381 cggcatacat cttggcgagc tcagcctgca tcgatatcga attcagcgcg ttattcatct
     1441 tgtgaagcgc ctctttcaga tgggcttttt cggtgtcatt caacggcatc ttgctacacc
     1501 cggtcttctt cgtattcgca tagtcggtta taaagggtct tgaggctgat cccgagcgct
     1561 tcagcggtaa gcttcttgtt gccgtcgaag tgctcaagag tcttctcgat gagggttttt
     1621 tctacgtccc ggatcgacat accggcacgg agcccctgcg aatcttcgga ccacttgggt
     1681 gggcggtcga agttgtccgg aagtgtgtct accttgtcct tgccccggtt caggatcgcg
     1741 gcacgcagca cggtgtgctt cagctccctg acatttccgg gccagtggta ggtctcgagt
     1801 ttcgacagga cccgaggcga aaagcgcgac ggcgtcccgt gctccttcga atacatctcg
     1861 agaaagtagt ttgcaagcag tggtatgtca ccttcacgtt ctcgcaacgg cgccacgtgg
     1921 atcggcaaca ccatcaggcg aaagaacagg tcttcccgaa acttgccctc gctgacttcc
     1981 ttttcgaggt ccttgtttgt tgcggcaatg atgcgcgcag tcatcggaat ttctttttcg
     2041 ccacctacac gcaccatcga gccggtttcg agaacccgca gcagggccac ctgttgatca
     2101 agtggcagct ccccgatctc atcgagaaac acggtgccgt tcttcgcccg ctcgaatatt
     2161 cctacccttt tcgccccggc gcccgtgaag cttcccttct catgcccgaa aagttcgctc
     2221 gccaccagtt cacgcggaat tgcgccgcag ttgactggta cgaacttggc gtcggaatgg
     2281 caggcgttgt gcagcgcctg tgcgaccaga tccttgccgg tgccgctctc cccggtgatg
     2341 aggatggtca cgtcagtggg accgtattcc gcaatcgact tcatcagcga cttcatcgcc
     2401 gccgattccc cgatcatgtt cgcaggccgg atcgcttcga tcgacgggat gcgcctggtg
     2461 ggcttgtcca gggccgcgga ctcgatgctc ttgatcagct ggttcttgtc aagcggcttc
     2521 accaggtagt cgacaacccg gccacgcacg gcccgtattg cggtctcgat agaagggtgg
     2581 ccggtgataa tcacggcttt gggaccgtca tccttgatca gttcaaggcc gctgccgtcc
     2641 ggcagggaca ggtcgacgaa cagcatgtcg aaattttcgc gccgcagcac tccgctcgct
     2701 tctgccacgc tgcccacggt tgaaacgtcg tgttcttctg ctctcagaag tgtctgggta
     2761 gccgacgtga agtcggtgtc atcgtcaaca agtagaattc gtgccatatt agaaatcgtt
     2821 gggtggtgac cccgccgtcg acccggcagg ggtcaccgtc acaggaacgc acagtatacg
     2881 gtaatggcac gatatttggg tcggggaaaa ccgggcgtgc atgccccggt ccgctcggct
     2941 gacgcctgac gcaccgccgg tcggtgcggc tccggcttgg cgcgccgagc cctggaaccg
     3001 ttacccggct cagaccgatt gaagtttcac caggcctgaa ggacgtaccg gttccgagcc
     3061 ctgcacaggc tggagtccat cgtggtacat cgcccaccga ttccttccat cgcgcttggc
     3121 ctcgtaaagg gccaggtcag atttcagcat ccactcctgc gtcgtacctg catggtccgg
     3181 catcagggcc accccgatgc tgatgctcgc gttgatctcg gaattgacgc ggaacggcgc
     3241 agcaaaactt ttcagcatct tgttggcgat ggccgggatg ttctcgggct tagtgaaccc
     3301 gttcaggaca acccagaact cgtcgcctcc tgctcgcccg acgacatcgt tctcgcggat
     3361 gcaatgtgca atgcgccgac agccctggcg caatatcagg tcgccgatcg ggtgcccata
     3421 ggtatcgttg atttccttga aatggtctat atcgatagtc atcaatgcag atgggatatt
     3481 cagccgcacg gctacgtcgt tggactcctt gatggcgctc aggagacccc tgcggttcaa
     3541 cgagccggta agctggtcga tacaggctat acgtttcagc ctctgctctc gctcatagcg
     3601 ctcgcgggcc agccgtatcg tgcgaaagat ccggttgggc gtggcttcgc tcttcggaat
     3661 gtaatcctgg atgccgagcc gaatcaggct caatgccgtc tccgacgaac tgtctcccgt
     3721 gatgacgacg ataaccagct cgggactgaa aatcttgagc tttgtcggtg cttctattcc
     3781 cgttgcatca ggcagaccga gatcaacgag tgcaacgtcg atccgcggtc cgtcctgcag
     3841 gagcttctgt gcggactgca ggtcggctgc ccggtacacc gagattcgag aatccagcat
     3901 tgacagcgcc cgcgacaacc cctgcgcaac aacgtcatta tcttcgacaa tcagaatttg
     3961 catagcttcc tgctcatctg ttgctttcac cgctcgaggt gtcacagccc cagccatccg
     4021 cgtgaagtga ccgacatttt gttcacgttc gatcggtctg gacgcgacag gccggcaacc
     4081 tgtccctgat tcgctttatt tgctgatatc cggtgcaaat cctgtgccag cttacccgcc
     4141 gccggaacca tggaagacgc cgcactgtgg tatgggaatt gcattcgctg gtgtggtcct
     4201 tgcgtgagtg cgtgtctttt gcgacccgct ccggtggaag aggcgcgaat actaatcgat
     4261 ctggcggcgg tagcaagccg tcagtgatat gcgacagcgc cagggcgcag tcgcgctgcc
     4321 acgttggaat ttgtcgcgaa atcgcgtcaa aaaagagcgc agcagacgca tagtttacaa
     4381 gcggatagca atttttacat tgaactgaag gagcatcgtg atggacgtta tcaataatga
     4441 gtggggattc ccaatgacga tgctcgcgtt gatctccgtc ctggcgattt ttggcaccgc
     4501 gctatggttc atactttccg cgatggacac gaacaagaag tttcgctggt tccagatcgt
     4561 tgggcggcgc gaacgacagg agcgcacaaa acgcactagt gaatgggaac aacaaaagcg
     4621 cccgaggtag agcgcgcgaa gcataggatt tattctgtta gcttattgcg gatccgaata
     4681 ccggaagccg ttgcttccta aacgccgttg cccctggagc agcgattgca atgcaggatg
     4741 cctggctgaa ctgcagttaa atgtctctcc gttcacactt gctgttgctg accctggccg
     4801 tgctcctgcc gctcgcgatc ttcggcgttg gtgcaacttt atgggttgca gatcgggagc
     4861 gtgatgcctt cgagcagggg gcgcgacagc gcacgcttgc actgattacg gcggtggata
     4921 cggaactggg cggacatgca ggaacgctgc gcgcgctcgc gagttcctac aacctcgaag
     4981 caggcaacct cccggcgttt catcgccagg ccatgcgttt gctgcagaca caggaaaact
     5041 ggcgaagcat tatcctcgcc atgccttcag gtcaacggat cctcgattcc tcgatgcccc
     5101 ttgacgcggc gctgtcagtg attgaccgca ccggtttcga tgaagcactt cgccgaaagg
     5161 agccgacaat cggcaacctc tctcccgagg attccgagta ccatttttcc gtgctgatgc
     5221 cggtgcagtc caccggacag gtatacatcc tgatggcgct ggtcgatccg caggcggtcc
     5281 tctcgctcct tacgccgcag cgtttacctc gcgactgggt gggcacggtc ctcgattcca
     5341 acaaccgggt cgtcgcgcgc acgttcaact cgagcgcgac cgtcgggaaa ccggcgtcgg
     5401 aatcgttgca ggctgctttg aggcaggctg cggaaggctg gtttcacggc accactatcg
     5461 agggtgccga cgtatatgca tcttttcacc agtcctcggt aaccggctgg acggtggcgc
     5521 tgggaattcc ctccgcggtg gttggcgcga caacgagcca tat
//
LOCUS       DASUOM010000563         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_660443,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000563 DASUOM010000000
VERSION     DASUOM010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660443"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFY27_11730"
     CDS             <1..362
                     /locus_tag="VFY27_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766231.1"
                     /translation="AVGTIANSVEAAGIWQSETAARQPQLLMTIWSVGGALFIAAQLA
                     WGTLIMGFSVAGSLTGVIPRWLARLGFVAVACCLVGALGIQSVMNGGWAQVPWFASIP
                     LFAVWVAIVSVLMIRKC"
     gene            590..1822
                     /locus_tag="VFY27_11735"
     CDS             590..1822
                     /locus_tag="VFY27_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766232.1"
                     /translation="MRSEASALRALTATIAVILCGCQEQVGSPSPVTAYDSAGVTVLD
                     FVVDTTIPAGLSLAREPRVTIGATDSDTFQLFRVVDAELMLDGTLVVANAGNSELMFF
                     DDSGTLVRKQGRSGTGPGEFQSISWIDRRSPDSLAVGDDRLRRVIVLDRTGVPIRTVS
                     IAGTAADASSSSVYAPQVRSMLADGSLLIASYSSLMPQAGPQRTTATLFRYDPTSGAL
                     SDLGSWPAAELHLIPSEGRLAVLLPLLARDFIIEASPTVVAAGTTDRPEISLLAPTGD
                     VSMILRWTGAHRQLTPEQLTRETQSRLEAFPDGPLREQRLREVEQISAHETVPAFGSI
                     RISDDGAIWIGGYALREDSTVAWLRVDPATREVARLAVPGAAQVLDATNDRIVLLIRD
                     DLDREVIAVHDLIRFNDR"
     gene            2099..2689
                     /locus_tag="VFY27_11740"
     CDS             2099..2689
                     /locus_tag="VFY27_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766233.1"
                     /translation="MDQSAAVDARGLRLTSWTAACWAFLYGLYRFYYAVGGEIGIPGT
                     PASQRQWLFINGAASMLLFAAAGAPIVLRSLWYMPRGRMILLALCWVITVTCVGHALI
                     GMVQRISSLTGSLTISYPFWQSIDRRQADLQALFFNEPWFLGEGLLWSGMAWAGAMRR
                     SPRRWIWYGSAAAAVAVYTTIGLLSAFGIIGRVVVG"
BASE COUNT          595 a         1034 c         1085 g          734 t
ORIGIN      
        1 ccgcggttgg cacgattgcc aactccgtag aggccgctgg catctggcag tccgaaaccg
       61 ctgcgcggca gccacagctg ctgatgacca tctggagcgt tggcggtgcc ctcttcatcg
      121 cggcacaact ggcctggggt acactgatca tgggcttcag cgtcgcaggt agcctgactg
      181 gagtgattcc tcgttggctg gcacgcctcg ggttcgtagc agttgcctgt tgcttggtgg
      241 gtgcgctggg gatccagtcc gtgatgaacg gtggatgggc acaggtaccg tggtttgcct
      301 ctattccgct ctttgcggtc tgggtcgcga tcgtgagtgt gctcatgatt cgcaagtgct
      361 gagcgctgtc gccatcggta tggcaggata gggacgctcc ggcgtataac actcgttggc
      421 cgcccggcgg ctgcgccgcc ttgccgccgg gtgaagggca gacatgctcg gcgcgtggac
      481 cggcggacac gtcccttcgt ggccgtggat cgctgacgct caccacggcc cctgcgggcc
      541 acgtctcgaa gcttgaacgt gagcagacac ttgagccgac ggatcagaaa tgaggagtga
      601 ggcaagtgcg ttgcgggcac tcacagcaac cattgccgta attctttgcg gctgtcagga
      661 gcaggtgggt tccccatcgc cggtaaccgc ctatgacagt gccggcgtta ccgtactgga
      721 tttcgttgtc gatacgacga ttccagctgg cctgagcctc gcgcgcgaac cgcgagttac
      781 aatcggggcc accgactcgg acacgttcca gctcttccgt gtcgtcgatg ccgagctcat
      841 gctcgatgga actctcgtcg tcgcgaatgc tggcaactcg gagctcatgt tctttgatga
      901 cagcggaacg ctggtcagaa agcaagggag atcgggcact ggtccagggg agtttcagtc
      961 gatcagctgg atcgaccgca ggtcacccga ttcccttgcg gtcggggacg accgcctcag
     1021 acgtgtcatc gtactcgatc gcacaggcgt gccgattcgt acagtaagca ttgctggtac
     1081 cgcggccgac gcatcttcca gcagcgtata cgcaccacag gtgagaagca tgcttgccga
     1141 tggatcactc ctcatcgcga gctattcgtc gctgatgcca caggcgggtc cgcagcgaac
     1201 cacagcgacg ctgttccggt acgatccgac cagcggtgct ctctcagatc tcggatcctg
     1261 gccagccgcc gagttgcacc tcatcccgag cgagggtcgc cttgccgtcc tgctcccgct
     1321 gctcgcacga gatttcatca tcgaagcctc tcccaccgtt gtcgctgcgg gcaccaccga
     1381 tcgccccgaa atcagcctgc ttgcgccgac cggcgacgtc tcaatgattc tccgatggac
     1441 aggggcacac aggcaactga cacctgagca gctgacccgg gagacacaga gcagactcga
     1501 ggcgttccca gatgggccat tgcgtgaaca gcgcctccgg gaggttgagc agatttcagc
     1561 tcacgaaacg gtccctgcgt tcgggtccat tcgcatctcg gacgacggcg caatctggat
     1621 cggcggctac gccctgcgcg aggacagcac cgtggcctgg cttcgtgtcg atcccgcgac
     1681 gcgcgaagtc gcacgtctcg ctgttccggg agcggctcaa gttctcgatg ccacgaacga
     1741 ccgaatcgtg ctcttgatac gtgatgatct cgatcgcgag gtcatcgccg ttcacgattt
     1801 gattcggttt aatgatcggt agcatcggat tccggttgcg cgcagcagct cggttgggca
     1861 accacggctc aagcgtccgc taacactcgc ctgagcccga cggcttgcgc cgcctgggta
     1921 gccgggtgaa ggatgggcgt gcttgggcgc gtagaccggc ggacatgtcg ccttcgtggc
     1981 cgtggatcac tgacgcccca cggcccctgc gggccacgtc tcaaagcgtg aacgttagat
     2041 accattgcgg gtgcaacgac acgaatgtca cgcattcggc ccctatactg agtcctgcgt
     2101 ggatcaatct gccgcggtcg atgcccgagg cctgcgcttg acgagctgga ccgcagcctg
     2161 ctgggcattc ctgtacggtc tctaccgatt ctactacgcc gttggcgggg agatcggcat
     2221 tcctggaacg cctgcatcgc agcgtcagtg gctgttcatc aacggtgctg ccagcatgct
     2281 gctcttcgcg gctgccggtg ctccgattgt tcttcgatcg ctgtggtata tgccgcgcgg
     2341 ccgcatgatt ctcctcgccc tctgttgggt catcacggtc acgtgcgttg ggcatgcact
     2401 gatcgggatg gtgcaacgaa tctcaagcct cacgggctct ctgacgataa gctatccctt
     2461 ctggcagtcg atcgatcgca gacaggccga cctccaagcg ctattcttca acgagccctg
     2521 gttcctgggc gaaggcttgc tttggagcgg aatggcgtgg gcaggcgcta tgcgtaggtc
     2581 cccgcgtcga tggatttggt acggaagcgc tgccgctgca gtcgcagtgt acacaacgat
     2641 cggccttctg agtgccttcg gaatcattgg acgagtcgtt gtcggatgac tcccggtgga
     2701 agggtggcac gtgagtatcg ctgcgtgtac gtcgcgtgac cgcttgccgc agcagcaccc
     2761 gaacggcagc tcacacgctt tgagcatgcc ggcttgcgcc gcctgggtcg ccgggtgaaa
     2821 cgtaggcttg cctggcgtgt ggccgccgga catgtcactt cgagctcgtg gatggttgac
     2881 gctcaccacg gcccctgcgg gccacgtcgg tcaacttgaa cgttatccgc aaccgggctt
     2941 ttggtttgga tcgcgagacg acgaaatcga caaacatgaa gagcttaagg tggattctga
     3001 tctcggcatt ggcgaccggc ttatcgttcc aatgccgtgg aggtgagcca gagaccggtg
     3061 gacaaacgcc atcatcgagt ttgtcgagcg aggagtcgaa ccacgattcg ctgacagacg
     3121 cagctgtgga gatcgttgag ttcctgcgtg gttccgctac gctcgacgcc aatttgctcg
     3181 cggattcaat ctcgttcttg gttgccgcgg aagggggcgg cgctgtacgc gttgtcgcaa
     3241 ggagtgttct tcgagctcgg agcgaatggc gtgttggtga gtacagtttg gtgccgccgg
     3301 gctcactcac tgaactcacg cttcagcctg gaagccatat caattgtcgt gagatgtccc
     3361 tgggctcgag atttcccgag ttcgacggcc tgcctcacgt cggtgtccgg ctcgccctgc
     3421 caggtgcagc tagctgtctg cagacatg
//
LOCUS       DASUOM010000564         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_167441,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000564 DASUOM010000000
VERSION     DASUOM010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167441"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFY27_11745"
     CDS             complement(<1..814)
                     /locus_tag="VFY27_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766234.1"
                     /translation="MATEETFPSVAAMLRERRPVAPVYCIYPEIYTTTAREFVAGFPG
                     RVLYAVKANDHPGIIRLLFDAGVRDFDCASVPEIARVRAACADATAYFMVPVRLRGAA
                     REAQEKYGVRHFMVDHPSGIEPLAREVGMSRVVVFARMAAHHQSAMQDLSLRFGAPPA
                     EVPDLLQQIAAQGAEPALAFNVGSAVTDPEAYSSAMELAASVLHKLPFRVRLLDIGGG
                     FPMSYPGFPVPPLEDYFERIVALRASLPLAPGGELMGEPGRALAAPGLSAVVE"
     gene            complement(823..2280)
                     /locus_tag="VFY27_11750"
     CDS             complement(823..2280)
                     /locus_tag="VFY27_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX5766235.1"
                     /translation="MTTLHTLDLLAIGIYFAVLLWIGVRVARAERGEEESESFLAADR
                     DMNLVQTTATTAATDLGGGFSIAMGGLGFTLGISGSWLIAISGLSVVMVSFLMVPKVK
                     RWADRVKGLTTGDLFEARFDRRTGTVAAVVVGLAWFTFVGGQVIAGGKLLQVTLSLDL
                     TLGVLLSGSVILAYTAMGGLKAVIYTDVFQFVVLFIGVLFIAVPLGLIEVGGISGIRE
                     HFSASPETASMIRWDAVGWKTALGWFFAIFPVWFISIAAMQRIVAARDERTARWSFFL
                     TGVPIEWPLFAVGSTLIGMIARMLMPDLADAELATPMVILELLPAGLAGFVVAAYIAA
                     VMSTADSALIGPVAIFTNDIYRKRIRPDASNRSLVRIARIATIVLGVLAIAIAYLVPN
                     VLDLILYAYTFGAAGLFFPMVGLLFWPRTTAKGAFFSMLAGGSSAVAWAAFGEPHGFA
                     ASYAGWVIGLPVLVVVSLLTEHGADENLKLFYDEK"
     gene            complement(2375..3400)
                     /locus_tag="VFY27_11755"
     CDS             complement(2375..3400)
                     /locus_tag="VFY27_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5766236.1"
                     /translation="MKVIYTEAQKRHYPKNFLSSGAPQPNPEVPERADRLLAAAQMAG
                     LSHKEPQDFGDAPLAAVHTPEYLQFLQHIYTRWQRIPEASPEVMPNIHPPRRGGAYPL
                     SAVGQVGYHVCDSSCPISADTWESARWSAMTAVQGAREILNGDSACYSLARPPGHHAS
                     QDLAGGFCYLNNSAIGAEVLRSQYDRVAILDIDVHHGNGTQEIFYERADVLTVSIHAD
                     PVRFYPFFWGHAAERGKGRGLGYNLNLPLPRGTSDDEYLQALDRAFLRVRAFSPGALV
                     VALGLDAFEGDPFQGLAITTDGFGRIAERIAGLQLPTLIVQEGGYLCDELGDNLKSFL
                     QVFASGS"
     gene            complement(3406..>3982)
                     /locus_tag="VFY27_11760"
     CDS             complement(3406..>3982)
                     /locus_tag="VFY27_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5766237.1"
                     /translation="GGLLQVPIALARGAHSVRREVIAKLKDQSGITVRLATEQDAATI
                     HALIVDLARATGLREKVTSKPQHFSRHGAGERAAFQALIAERAGQALGLCLFFYSFSS
                     WRGELGIYVQDLFVSDAARGSGLGRRLMRETARLGKQRGATYLRLSVAKENTAAQEFY
                     RTIGMTEAADECIYQVAGTAFRQLASAAGTD"
BASE COUNT          751 a         1312 c         1270 g          649 t
ORIGIN      
        1 cctcgacgac cgccgagaga cccggcgcgg ccagtgcgcg ccccggctcg cccatcagct
       61 cgccgccggg agccaacggc agtgacgccc tcagtgccac gattcgctcg aagtagtctt
      121 cgagcggcgg caccgggaag cccgggtagg acatcgggaa tccgccaccg atgtcgagca
      181 ggcgcacccg gaacggcagc ttgtgcagca cgcttgccgc aagctccatt gcggacgagt
      241 acgcctcggg gtccgtcaca gcggaaccga cattgaaggc gagcgcaggt tcggccccct
      301 gcgccgcgat ctgttgcagc aggtcgggta cttcggcggg cggcgcgccg aaccttaacg
      361 aaaggtcctg catcgcggac tggtgatgcg ccgccatgcg agcgaacacg acgacgcggc
      421 tcatgccgac ttcgcgcgcc aacggctcga taccgctcgg gtgatcgacc atgaagtgcc
      481 gcacaccgta tttttcctgc gcctcgcgag cagcgccacg aagccgcacc ggcaccatga
      541 agtacgcagt tgcgtccgca caggcggcac gtactcgcgc gatctcgggc acggacgcgc
      601 agtcgaagtc acgcactcct gcatcgaaca gcagccgaat gatgcccggg tggtcgttgg
      661 ccttcaccgc gtagagaacc cggccgggga agcccgcgac gaactcccgc gcggtggtcg
      721 tgtagatttc ggggtatatg cagtagaccg gcgccaccgg acgccgctcg cgcagcatgg
      781 ccgcgacact cgggaaagtc tcttccgtag ccattcttcc gttcactttt cgtcgtaaaa
      841 gagcttgagg ttctcgtccg ccccgtgctc cgtcagcagc gacactacga cgagcaccgg
      901 gagccctatg acccagccgg cgtaggaagc ggcgaaacca tgcggttcgc cgaaagccgc
      961 ccaggcgaca gccgaggagc cgccggcaag catgctgaag aatgcgcctt tcgccgttgt
     1021 tcgcggccag aacaggagcc cgaccatcgg gaagaacaat cccgcggcgc cgaacgtgta
     1081 cgcgtaaagg atgaggtcca gtacgttcgg caccaggtag gctatggcaa tggcgagcac
     1141 tccgagcacg atcgttgcga tacgagcaat gcgaacgagc gaacggttgc tcgcatccgg
     1201 ccggatgcgc ttgcggtaga tatcgttggt gaaaatggca acgggaccga tcagtgccga
     1261 gtccgcggtc gacatcacgg ccgcgatata ggctgcgacg acaaagccgg cgagtcccgc
     1321 cggcaacagc tcgaggatca ccatcggcgt cgccagttcc gcatcggcaa ggtccggcat
     1381 caacatgcgg gcgatcatgc cgatgagcgt gctaccgacc gcgaacagcg gccactcgat
     1441 gggcacgccg gtcaggaaga acgaccagcg cgccgttcgc tcgtctctcg ccgcaacgat
     1501 gcgctgcatg gctgcgatgg agatgaacca caccgggaag atggcgaaga accagccgag
     1561 cgcggttttc catccgaccg cgtcccagcg aatcatcgat gcggtttcag gcgatgcact
     1621 gaagtgctcc ctgatgccgc ttatgccgcc gacttcaatc aggccgagcg gcacggcgat
     1681 gaacagtacc ccgatgaaga gcacgacaaa ctggaaaacg tcggtgtaga tcaccgcctt
     1741 cagtccgccc atcgcggtgt acgcgagaat gaccgaaccg gagagcagca cgccgagcgt
     1801 gaggtcgagc gatagcgtca cctgcagcag cttccctccg gcgatgacct ggccgccaac
     1861 gaaggtgaac catgcgagtc ccaccacgac cgcggcgacc gttccggtgc ggcgatcgaa
     1921 gcgtgcctcg aacaggtcgc ccgtcgtcaa acctttgacc cgatccgccc agcgcttcac
     1981 cttcggcacc atcaggaacg acaccatgac cacgctcagt cccgaaatgg cgatcagcca
     2041 tgagccggag atgccgagcg tgaacccgag cccgcccatg gcgatgctga acccgccgcc
     2101 caggtccgtc gcggccgtgg ttgccgtggt ctgcacgagg ttcatgtcgc ggtcggcggc
     2161 gaggaaggat tcgctttcct cctcgccgcg ctccgcgcgc gccacacgca cgccgatcca
     2221 cagcaggacg gcgaagtaga tgccgatggc gaggagatcc agcgtgtgca gggtggtcat
     2281 gttgcggtcg tcgcggggta ggagcccgcc atgcggacga gtggaatacc cgatgtgggg
     2341 ctgcggatcc atcgccccgc gctatcgata atattcaact cccgctcgca aacacctgca
     2401 gaaacgactt gaggttatcc ccaagctcgt cgcacagata accgccttcc tgcacgatca
     2461 gcgtcggcag ctgcaagccg gcaatgcgtt ccgcgatacg cccgaagcca tcggtcgtga
     2521 tggccaaccc ctggaacgga tcgccttcga aggcatcgag acccagtgcg acgaccagcg
     2581 cgcccggaga aaacgcccgg accctcagga acgctcgatc cagcgcctgc aggtattcgt
     2641 cgtcgctcgt gccgcgcggc aacggcaggt tgaggttgta accgagcccc ctgcccttcc
     2701 ctcgttccgc cgcatgtccc cagaagaacg ggtagaagcg caccgggtcg gcatggatcg
     2761 acacggtcag cacgtcggcc cgctcgtaga agatttcctg cgtgccgttg ccgtgatgca
     2821 cgtcgatgtc gaggattgcg acgcgatcgt attgcgagcg caggacttct gcgcctatgg
     2881 cgctgttgtt caggtaacag aagccgcccg cgaggtcctg cgacgcatgg tgtcccggcg
     2941 ggcgcgcgag cgagtagcag gcgctgtcgc cgttgagaat ttcccgtgca ccctgtactg
     3001 cggtcattgc gctccagcgc gcgctctccc aggtatcggc agagatcggg caggagctgt
     3061 cgcagacgtg atagccgacc tgcccgaccg ccgacaacgg gtacgccccg ccgcggcggg
     3121 gcgggtggat gttcggcatc acttccgggg aagcctcggg aatacgctgc cagcgggtat
     3181 agatgtgctg caggaactgc aggtactcgg gagtgtgcac agccgccagg ggtgcgtcgc
     3241 cgaagtcctg cggctccttg tgggagagac ccgccatctg cgctgccgcc agcaaccggt
     3301 ccgcccgctc cggcacttcg gggttcggct gtggggcgcc ggaggacagg aagttcttcg
     3361 ggtaatgcct cttctgcgct tcggtgtaga tgactttcat gcgcctcagt ccgtaccggc
     3421 tgcagacgcc agttgccgga aagcggtgcc ggccacctgg taaatgcatt cgtctgccgc
     3481 ctcggtcatg ccgatcgtcc ggtagaactc ctgcgcagca gtgttttcct tcgcgaccga
     3541 cagcctgaga tatgttgcgc cacgttgctt cccgagtcgc gccgtttcgc gcatgagcct
     3601 gcgcccgagt ccggagccgc gcgccgcatc ggaaacgaac aggtcctgga cgtagatgcc
     3661 gagttctccg cgccacgacg agaaactgta gaagaacagg cacagaccga gcgcctgtcc
     3721 tgcgcgctcg gcgatgagcg cctggaacgc ggcgcgttcc ccagcgccgt ggcgcgaaaa
     3781 atgctgcggc ttgctggtga ctttctcccg cagcccggtt gccctcgcca ggtcgacgat
     3841 aagcgcatgg atggtcgcgg catcctgctc cgttgccagc cgcacggtga taccgctctg
     3901 gtctttcagc ttggcaatca cttcgcgacg cacgctgtgg gcgccacgtg ccaacgcaat
     3961 tggcacttgt aggagcccgc cg
//
LOCUS       DASUOM010000565         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_448544,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000565 DASUOM010000000
VERSION     DASUOM010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448544"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFY27_11765"
     CDS             <1..198
                     /locus_tag="VFY27_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5766238.1"
                     /translation="QGRGTEEDLDKLKQVADKIEGHTICALGDAAAWPVQSFLRHFRH
                     EFEFMVRHNGRSIVDNVAAAA"
     gene            206..2386
                     /gene="nuoG"
                     /locus_tag="VFY27_11770"
     CDS             206..2386
                     /gene="nuoG"
                     /locus_tag="VFY27_11770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5766239.1"
                     /translation="MSDDVINFEVDGQPMQGKKGQMIMEVTDHEGTYIPRFCYHKKLS
                     IAANCRMCLVEVEKAPKPLPACATPIGDGMKVFTKSPKAIAAQRATMEFLLINHPLDC
                     PICDQGGECELQDLAMGFGRDISRYYDGKRSVKDQDIGPLISTDMTRCIHCTRCVRFG
                     EEICGYQQLGTIGRGEWMKISTYIAQTIDHELSANIIDLCPVGALNNKPYRYSARAWE
                     MMQHATISPHDCVGSNMYVHSLRGTIKRVVPKENEAINETWLSDRDRFSYEGVYSGDR
                     LLVPQLKENGSWRDIDWEDALQLLAEKLSGRRGDKVGILASPSATVEEAWLLARLADG
                     LGSSNLDHRLRRRNFVDQQNDPVFPWLGCDIAAIQNLDAVLVVGSNLRQEAPILAHRV
                     RKAAIQGARIAMVNPQVYPYFFDVAPYLSGEMLAGQLAGIAVSASNGQLPDSVRALCE
                     DIQPTDTQREIARLLTDASDGLVLLGALAARDADFAAIRVLGAAIANLTGTRLGYLSD
                     GSNAAGAHLAGLLPHRRAGGIAREAAGLDAGRMLDAELDALLLFNVEPSHDHANGGDA
                     VARLGARQGFVAALTSFASPELLQGADLLLPVGTFAETSGTYVNCEGRWQSFGGVASA
                     LGEARPPWKVLRVLGNLLNLDGFDYLTSEAVRDELKAQLGDAAPDNAYRGSGPVERAT
                     ATSLDYDVPMYDVDAVVRRAVALQLTPEAARGRGDAAERQESAA"
     gene            2383..3465
                     /gene="nuoH"
                     /locus_tag="VFY27_11775"
     CDS             2383..3465
                     /gene="nuoH"
                     /locus_tag="VFY27_11775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5766240.1"
                     /translation="MSWLIPDFVANWWNIVPPTLQQVTVITTQIVAITICIILTVAFA
                     TYFERKVIGFMQGRIGPNRVGPLGLGQPFADVIKLLIKEVIVPRESNRFLFIIAPLFA
                     IIPALATWAVIPLTENFVVADIDAGLLYVLALTSMGVYGIILAGWATNSKYAFLGAMR
                     SAAQIVAYEIAMGFALVGVLMAGGSLNLGEIVRAQSGGVSNWFLFPLLPLFIVYFVSG
                     VAETNRAPFDVAEGESEIVAGFHVEYSGVAFAIFFLAEYANMVLISALTAIFFFGGWA
                     SFFEGWTFLGEDNFLREPSFFWLALKIAFFIFTFFWLRATFPRYRYDQIMRLGWKVFI
                     PVTLVWIAVEALMSWINWGPWARLAG"
     gene            3469..>3884
                     /gene="nuoI"
                     /locus_tag="VFY27_11780"
     CDS             3469..>3884
                     /gene="nuoI"
                     /locus_tag="VFY27_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5766241.1"
                     /translation="MDKVKGIFKTLVLWELWQGLGTTLRNFFRDDVTVQYPEEKTPQS
                     FRYRGLHAQRRYANGEERCIACKLCEAVCPALAITIESEVGDDGTRRTTRYDIDLFKC
                     IYCGFCEEACPVDAIVETRIHEYHMETPGENIMTKD"
BASE COUNT          735 a         1149 c         1249 g          751 t
ORIGIN      
        1 caggggcgcg gcaccgaaga ggatctggac aagctgaaac aggtcgcgga caagatagag
       61 ggacacacca tttgtgcgct cggcgacgcc gcggcgtggc cggtacagag tttcctccgc
      121 cacttccgcc acgagttcga gttcatggtg cggcacaacg gacgcagcat cgtcgataac
      181 gttgccgcgg ccgcatgagg aagggatgag cgacgacgtc atcaatttcg aggtcgacgg
      241 ccagcccatg cagggcaaga aaggccagat gatcatggag gtcaccgatc acgaagggac
      301 ctacattccg cgtttctgtt atcacaagaa gctgagcatc gcggccaact gccgtatgtg
      361 cctcgtggaa gtagagaagg ctccaaagcc gttgccggct tgtgcgaccc cgattggcga
      421 cggcatgaag gtcttcacga aatccccgaa ggcgattgcg gcgcagcggg cgacgatgga
      481 gttcctgctc attaaccatc cgctcgactg tcccatctgc gaccagggcg gcgaatgcga
      541 actgcaggac ctggcgatgg gttttggcag ggacatttcg cgctattacg acgggaagcg
      601 ttcggtgaag gaccaggaca tcggccctct tatctcgacc gacatgacgc gctgtatcca
      661 ctgcacgcgc tgcgtgaggt tcggcgagga gatttgcggt taccagcagc tcggcacgat
      721 cgggcgcggc gagtggatga aaatcagcac ctacatcgcg cagaccatcg accacgagct
      781 gtcggcaaac atcatcgatc tctgtcccgt cggtgcgttg aacaacaagc cttaccgcta
      841 cagcgcccgc gcatgggaaa tgatgcagca tgccacgatt tcgccgcacg actgcgtcgg
      901 ctcgaacatg tatgtgcact ccctgcgcgg caccatcaag cgcgtggtac cgaaagagaa
      961 cgaggcgatc aacgagacgt ggctgtccga tcgtgacagg ttcagttacg aaggcgtata
     1021 cagcggcgac cggctgctgg taccgcagct gaaagaaaac ggcagctggc gggacatcga
     1081 ctgggaagac gcattgcagc tgcttgcgga gaagctttcg ggcagacggg gagacaaggt
     1141 cgggatactg gcgtcgccga gcgcgaccgt cgaggaggcg tggctactgg cccgccttgc
     1201 ggacggcctg ggcagcagca acctggacca ccgcttgcgg cgccggaact ttgtcgacca
     1261 gcagaacgat ccggtttttc cctggctcgg ctgcgacatc gccgccatcc agaaccttga
     1321 tgctgtgctg gtagttggct cgaacctgcg ccaggaagca ccgatccttg cgcaccgggt
     1381 gcgaaaggcc gcgattcagg gcgcgcgcat cgcgatggtc aatccgcagg tctatcccta
     1441 tttcttcgac gtcgcgccgt acctgtcggg agagatgctg gccggccagc tggccggtat
     1501 tgcggtgtcg gcgtcgaacg ggcaactgcc ggattcggtg cgagcgcttt gcgaagacat
     1561 ccagcctacc gatacacagc gggagatcgc gcgcctgctt acggatgcca gtgacgggct
     1621 ggtgctgctg ggcgcccttg cggcgcgtga cgccgacttt gccgcgatcc gcgtcctggg
     1681 tgcagcgatt gcaaacctca ccggtacccg cttgggctac ctgtcggacg gttcgaacgc
     1741 ggcgggagcg cacctcgcag gcctgctgcc gcatcgcaga gccggtggga ttgcgcgtga
     1801 agccgcggga ctggacgctg gccgtatgct ggatgccgag ctcgatgctt tgctgttgtt
     1861 caatgttgag ccgagccacg atcatgccaa cggcggggat gctgtcgcgc ggctgggcgc
     1921 gcggcaaggc ttcgtggcgg cattgacgtc ctttgcctcc ccggagctcc tgcagggagc
     1981 agacctgctg ttgcctgtcg ggacgtttgc cgaaacctcc ggcacctacg tgaactgcga
     2041 gggtcgctgg cagagcttcg gcggagtcgc gagcgcgctc ggcgaagcgc gaccgccatg
     2101 gaaggtcctg cgggtcctgg ggaacctgct gaacctggac ggcttcgact acctcaccag
     2161 cgaggcggtg cgcgacgaac tcaaggcgca gctcggcgat gcagctccgg acaacgcata
     2221 ccggggtagc ggcccggtcg aacgtgccac cgcaacgtcc ctcgactatg acgtgccgat
     2281 gtacgatgtg gatgcggtgg tgcgccgcgc agtcgcgctg caactaacgc cggaagcggc
     2341 gcgtggccgc ggcgacgcgg cggagcggca ggaatccgca gcatgagctg gctcataccg
     2401 gatttcgtcg ccaattggtg gaacatcgtt ccgcctacgt tgcaacaggt gaccgtcatt
     2461 acgacccaga tcgtcgcgat cacgatctgc atcatcctga cggtggcttt cgcgacctac
     2521 ttcgagcgaa aggtgatcgg cttcatgcag ggccgaatcg gcccgaaccg cgtcggtccg
     2581 ctggggctgg ggcagccatt cgccgatgtt atcaagctgc tgatcaagga agtgatagtt
     2641 ccgcgggaat cgaaccgatt cctgttcatc atcgcaccgt tgttcgccat cattcccgca
     2701 ctcgcaacct gggccgtcat cccgctgacc gagaacttcg tcgtcgcgga catcgatgcc
     2761 ggcttgttgt acgtgctggc cctgacctcg atgggtgtct acggcatcat cctggcggga
     2821 tgggccacga attcgaaata tgcattcctg ggtgcgatgc ggtcggcggc gcagattgtc
     2881 gcctacgaaa tcgccatggg ctttgcgctg gttggcgtgc tgatggccgg aggtagcctg
     2941 aacctgggcg agattgtccg ggcgcagtca ggcggcgtca gcaattggtt cctcttccca
     3001 ctgctgccgc tgttcatcgt gtacttcgta tcgggagtgg ccgagactaa ccgcgcgccg
     3061 ttcgacgtgg cggaaggtga atccgagatc gtggcgggtt tccacgtcga gtactccggc
     3121 gtcgctttcg ccattttctt cctggccgaa tacgccaaca tggtactgat atccgcgctc
     3181 accgccattt tctttttcgg cggctgggca tcctttttcg aaggatggac gttcctcggc
     3241 gaggacaact tcctgcggga gccgagcttt ttctggctgg ccctgaagat cgcgtttttc
     3301 attttcacct ttttctggct gcgcgccacg ttcccccggt atcggtatga ccagatcatg
     3361 cggctcggct ggaaagtatt cattcccgtg accctcgtgt ggattgcggt cgaggccttg
     3421 atgtcctgga ttaactgggg cccctgggcg aggctggcag gctaggcaat ggacaaagta
     3481 aaaggcattt tcaagacgct cgttctgtgg gaactgtggc aggggcttgg aacgaccctc
     3541 cgcaacttct tccgtgacga cgtcacggtg cagtacccgg aggagaagac gccgcagtcg
     3601 ttccgctacc gtggactgca tgcccagagg cgctacgcca atggcgaaga acgctgcatt
     3661 gcctgcaagc tctgcgaagc cgtctgtccc gcgctagcca ttaccataga gtcggaagtc
     3721 ggggacgacg gaacgcgccg gaccacgcgc tacgacatcg atcttttcaa gtgcatctac
     3781 tgcggcttct gcgaggaagc ctgcccggtc gatgcaatcg tggagacgcg aatccacgaa
     3841 taccacatgg aaacacccgg cgagaacatc atgacgaagg acaa
//
LOCUS       DASUOM010000566        15780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1162161,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000566 DASUOM010000000
VERSION     DASUOM010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162161"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFY27_11785"
     CDS             <1..315
                     /locus_tag="VFY27_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX5766242.1"
                     /translation="ASEHPDLWRLVFEHQAPRGLPTPERMKVRRDVMFELVADNLREH
                     SPDHSEQEVNHTATALWSGVHGICILALTGKLYLGGAFSMNKLIDTLIDAVLNDFKHK
                     GA"
     gene            complement(334..1485)
                     /locus_tag="VFY27_11790"
     CDS             complement(334..1485)
                     /locus_tag="VFY27_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5766243.1"
                     /translation="MIRLIQLTAMLALAAPALMWANPQTTEPDRHIANQPPRIQLAIL
                     LDTSNSMDGLIDQARQQLWQVVNEFSKAKRNGVTPVFEVAVYEYGNTGIDARLGHVRQ
                     VVGLTRELDRVSEALFSLTTNGGDEYCGHVIAQAVNQLQWSASPNDVKAIFIAGNEPF
                     TQGPTPFTEAIAEANVRGIVVNTIHAGDYQTGLSEGWQQGALLAGGNYMSIDHNQAVV
                     HIEAPQDGEIANLNARLNETYIPYGREGDAGAARQQEQDDKSYDVSAGLLAERAESKA
                     TALYDNSGWDLVDAVEAGKTDLDELESDALPEEMQSMSLEARKALVDEKANERAELKA
                     QIVELGRQRDAYVAEQRNIEAEQNGSTLNEALMESVREQAADRSFVFTE"
     gene            1633..2664
                     /locus_tag="VFY27_11795"
     CDS             1633..2664
                     /locus_tag="VFY27_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5766244.1"
                     /translation="MTTRSKRLLPILLGTFVCSANGAAEPADWGAIEVAGNSVQPGTI
                     GRFPAISQRGFEATYLNLPVYVAHGAEAGPTLCVTAAIHGDETNSVEIARRVFAETDA
                     TGLKGTLYMLPFVNADGFRNRKREMTDGRDLNRTFPGSANGSVASLVANFVFTDLTSR
                     CTALIDLHTGSNLRSNYPQIRVDLENEDAKELAVHFGVGMILGGGGPDGSLRGEMVKA
                     GIPAIIYEAGGPLLFQEEEVVRGTEGVRNVMHYLGMMEIPGYKASQPKIYEKTRWVRV
                     PRGQGGFFFPELSMGAKVEEGTRMGYVVDPVTDEQHVITSKDAGQIIGMTLPQPVLSG
                     YPLFHLGVE"
     gene            complement(2700..3764)
                     /locus_tag="VFY27_11800"
     CDS             complement(2700..3764)
                     /locus_tag="VFY27_11800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5766245.1"
                     /translation="MIVCLSAVFCPASQAAELAWANVPERSVPAHLDRAIAQGDAMPR
                     LHSLLVSQHGELLLERYFNGRGPDDLANVKSVSKSILSALVGIAIEQRILDGVEITLT
                     RYFGELLTEVGPDKGSITLEDLLTMRSGLRATSNGNYSAWVLSDNWVVSALRQPLETT
                     PGQEMQYSTGNTHLLSAILTTASGRSTLEFAREALTEPLGFELAPWPRDPQGIYFGGN
                     DMRLTPRQMLAFGELYLNGGRRGERQIVPAWWVEASIQPRSRSPHGGARYYGYGWWIC
                     ELAGYQVPHAWGHGGQFIMLVPELDLVIVTTSSTNAGAVAHDHANQVYQWLQQIIHLV
                     ATAPSVPAANDRETVAHLRR"
     gene            complement(3952..5751)
                     /locus_tag="VFY27_11805"
     CDS             complement(3952..5751)
                     /locus_tag="VFY27_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5766246.1"
                     /translation="MDLQQIIFLAILAVALVLLVTEWIRLDLTGVFIVVALAGTGLLS
                     PSEALSGFSSEPAILVASMFVLSGGLSYTGLTEALGRWIGRLSGESLSRAVLVIMPSV
                     ALMAAFSHHLMITAMMLPVILGLNRTHNIPSSRLMMPMAFAASLGTTMTVVAAPAFLV
                     ARNLLQRTGEHDVGIFDITPIGIVLTLIGTAYILLLGRWLLPTREGATAEEDRFRLER
                     YYTELVVLEDSLYKGKSMQEFQEEHEERFQVVDWLRDGQSRARPWQRKEIAPHDLLLV
                     RASPDELATLEKQQGLALHAVMQYGEVIPDEKAKDLPGSERLVQAVVGPDSELIGRNI
                     GSVDFLARYDVVVVGLWRKRGFMRKELSKVVLKAGDLLVLWGDQKALDRLADNPSFLL
                     LLPFSARDRKLGKGWLAGLIMGGSVVAATTGVLPVTIAFMTGAAVMVLTGCLSLKQAY
                     DSVDTRIFVFIAGAIPLGIAMEQTGTAALFAGWLAELVGPWETTWVLLALFLTASLFT
                     QILSDTATTILLGPVALGMAALLDISAPAMVFSVAMGAVVAFLTPIGHHGNLLVYEPG
                     DYRFADFVRVGTPLTLLLGLAVAFLAPVVWPGL"
     gene            complement(5837..7723)
                     /locus_tag="VFY27_11810"
     CDS             complement(5837..7723)
                     /locus_tag="VFY27_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5766247.1"
                     /translation="MEVPGGTPIYTFGEFQLHVARQALRSEAEDRWIPLTPRVFETLL
                     FLVEHPGELLDKARMMAAIWPDVVVEENNLDQNISTLRRLFGERRGEPRYIATVRRRG
                     YRFVAPVSRLAEGNGRPAARSNGVEPNPATANNRFDLVKWWGLAAAAGLALAAVLAEW
                     PGERPVPVSDLPAATLAVLPFRPLTATDRNESLELGMAESLIARLSNSRLVVSPLSAV
                     RRYADPGQDAVAAGRELGVDAVLEGHLQRDGERLRVSVRLMNAGDGHQLWADRYDENF
                     TDIFSVQDAIATKVRAALAPELTGEETPPFRRYTEDAEAYQLYASGRFHRRRGNEAGL
                     REALGFFHEAVERDPDFALAYVGLAEGYAGLGVFGIAAPHDVFPRAQQAVHRALELAP
                     ELGEAYASLGHVKVQYEHDWSGAERAYARAIDLSPNYAPAHQWLGIYRSFSGRFEEGL
                     ERMHHAQTLEPASPLYGALIGMLLIYQRRYDEAIEHLEKTLELDPSLATTRTYLALAY
                     LRRGEYDRALQHLDEASSLAPGSAAYRGQAHALAGRRKEAEAEIQRLVADSQQRYVSA
                     YDIATIHASLGNRDEAFTWLERAFAERSQLIAWLPWDPVFDGMRSDPRYSGMAGRLNI
                     DIQD"
     gene            7863..8402
                     /locus_tag="VFY27_11815"
     CDS             7863..8402
                     /locus_tag="VFY27_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766248.1"
                     /translation="MKTLAGNALFAVLVAGMLAVQPAKGEGQEDVLQQHASTDWDKLW
                     WGKDPIEGVWNVRVVITDCASGLPLGPPPFDAMAMFNANGTFQDTNSNSPALQVRSEA
                     LGYWKYVRGNKYRFAFKAFHFDLSGMYVGYNIVRHDVVLADNGRSYISTGTVEFFNPD
                     GTTRPPVMGCSEAKATRFK"
     gene            complement(8609..9178)
                     /locus_tag="VFY27_11820"
     CDS             complement(8609..9178)
                     /locus_tag="VFY27_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766249.1"
                     /translation="MKSQYRNTWIALTAATPLLVMAAAPAAAEERVCRGTIGATTVDN
                     LRVPDNANCVLKGTRVKGTIKVESNATLRAEDVVVIGNVQAENAKNVRVLEGSRVGGS
                     VQVKQGGGATVSDSRVDADIQYESNSAMLRVLRNVVGGNVQAFQNVGGVEIRRNTIDG
                     NLQCKANSPPPVGGRNVVDGNKEDQCRRL"
     gene            complement(9360..10706)
                     /locus_tag="VFY27_11825"
     CDS             complement(9360..10706)
                     /locus_tag="VFY27_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5766250.1"
                     /translation="MKKRILVIDDEPAVRFGLREYLETQGYEVHEAGSCQQALEVFLS
                     TSPDAAVIDYKLQDGDALTLLPRLREIDATVPLIVLTAHGSIDLAVQAIKEGAEQFLT
                     KPIELPAFQVILRRLLDARRAHRAHLVGSARERRAAIDPFLGTSAAIRELEIHARKVL
                     DAESPILICGETGTGKGVVAAWLHRHGPRSREAFVDLNCAGLTREFLESELFGHEKGA
                     FTGAVTRKQGLLEIAHGGTVFLDEIGDVDSQVQPKLLKVLEEKRFRRLGDVRERFVDT
                     RLIAATHADLDALMRDGRFRSDLYFRISAIPLVVPPLRERDADVVILAKALIGQLAAD
                     LGRTAPAFTAAAEKALLAYRWPGNVRELRNVLERALLLGSGDAIDEKDLRFGTAHAMP
                     AQDDSMLTLEELERRHIERALDATKGKVADAARRLGIPRSTLYQKLKDYGLKPSRN"
     gene            complement(10703..12604)
                     /locus_tag="VFY27_11830"
     CDS             complement(10703..12604)
                     /locus_tag="VFY27_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766251.1"
                     /translation="MTHGNWRFSVTRKVALGLALIMAVGMASMLSIYRGLERVGVAHE
                     QVAAVQAPIVAAAYGMEVNVNGAALHILTYLAGRRPEDRTEAEDDVGDFTSYHATYLE
                     LVMTDRERELGRRIYHQHSRFATLAGELMDLADRQEELYAAITEDMEEIDYVLDNRLQ
                     PRLFREAELRRVGIGAAVASANFEAETAEVGQWAANYHRGPTPSARRTILAKLTVMES
                     ALHSFLGFDLAGEDRQLEPTLRKLVGRVAVHVREVLALEDQIFEGRNRLIGLRESMDD
                     LLDENIQVLAVGGMDEPKAQAKAAANEVLVAMKLLVPMFLLAAGVIGFMLIQVIRKPL
                     GKLYRGTLAVTGGDLSYRIPPASNDEFGDLARQYNAMVDQLEATTVSRDRLKQEMAER
                     ERAEHERESLQAELRRSETMSTMGALVAGVAHEVRNPLFAISSTLDALTARLGERSEY
                     SRFIENLQGQVSRLGSLMADLLAYGRPTASEFSTGSIDAIVARALESCGTLARETGAT
                     IEVRLDPDPPPVRLDPGRLGQALHNLVDNALRHAPQGSIVTIETRRIEHRGGSWIECA
                     VSDRGPGFSAADLPHVFEPFFTRRHGGTGLGLALVQRIVSEHGGEVLAGNHEEGGAVV
                     TIRLPVAQA"
     gene            12834..13193
                     /locus_tag="VFY27_11835"
     CDS             12834..13193
                     /locus_tag="VFY27_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766252.1"
                     /translation="MQRAFARFSHIGGYACLPKGIVTALANLVRAINCYHSNLVEGHN
                     THPIDIERAPKKDYSADAEQRNLQLEAEAHITVQQWIDEGALRGRATSVDSILGNPPT
                     LKSTDAFAKCCARSCCS"
     gene            complement(13409..13663)
                     /locus_tag="VFY27_11840"
     CDS             complement(13409..13663)
                     /locus_tag="VFY27_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766253.1"
                     /translation="MLKWTATFFFLMIACGVVGFTNAQGEMVEAGFLFLLFFYLTVVS
                     AFLSGLSGNPPLAWLRDLIRDRAPATRVRERSASVDFFRR"
     gene            14846..>15780
                     /locus_tag="VFY27_11845"
     CDS             14846..>15780
                     /locus_tag="VFY27_11845"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5766254.1"
                     /translation="MSYLWPRKPIVLPLLSAALFGGCGGTPSGPTTAVSPALGDNFAG
                     VVTEPFGVFAGVEYVRHTGVFEGESTLGAFRVPFEVVAPADPDLGENTLLFEPPHSGF
                     GTVGRNLVLGPDLIFGRGLRFASVGVGNLSDLHILDPSIPDLLLAGAAFVPADPENLP
                     AGSIIDEEIIALFLAALESDEDMAAMLGSIDRRYAYGISQSAAAISELLFDPTGLGQE
                     VFDLSFLHAAHWRPVFETRGAFHNLPDGSPVPVAFDLPTGVGRVMIVNSEGDALVSDS
                     EFYQCAIHRADFRLYEVSGAAHVPTSDAVPLGNNPL"
BASE COUNT         2923 a         5032 c         4904 g         2921 t
ORIGIN      
        1 gcctccgagc acccggacct gtggcgcctc gtgttcgagc accaggcgcc cagggggctg
       61 ccgacaccgg aacggatgaa agtgcgccgc gacgtcatgt tcgaactcgt cgcagacaat
      121 ctccgcgaac attcgcccga ccactcggag caggaagtaa accacaccgc cacggcactc
      181 tggagcggtg tgcatggaat ctgcattctt gcgctgaccg gcaaactcta cctgggaggc
      241 gcattctcga tgaacaagct gatcgatacg ctgatcgacg ccgtcctgaa cgatttcaag
      301 cacaagggtg cctgacgtac agtcggtgtc gcgctactcc gtgaaaacaa agctccggtc
      361 tgccgcctgc tctctcacgg actccatcag cgcctcgttc agcgtactgc cattttgctc
      421 ggcttcgatg ttgcgctgct cggccacgta ggcatcacgc tgccggccca gctcgacgat
      481 ctgcgctttc agctcggccc tttcgttcgc cttctcgtcc acgagcgcct ttcgcgcctc
      541 gaggctcatg gactgcattt cctcgggtag cgcgtcggac tccagttcat cgaggtcggt
      601 tttgccggcc tcgacggcgt ccacgagatc ccatcccgag ttgtcgtaca aagcagtcgc
      661 cttggattca gcgcgctccg ccagcaggcc cgcggacaca tcgtagctct tgtcgtcctg
      721 ttcctgctgc cgagccgcgc ccgcgtcgcc ttcccggcca tacggaatat aggtctcgtt
      781 cagacgggcg ttcagattgg cgatctcgcc gtcctgcggc gcctcgatgt gcaccaccgc
      841 ctggttgtgg tcgatgctca tgtagttgcc gccggcgagc agcgcaccct gctgccagcc
      901 ctcggagaga cccgtctggt aatcgccggc gtggatggta ttcaccacga tgccgcgtac
      961 attggcctcg gcgatcgcct cggtgaacgg cgtcggtccc tgggtgaaag gttcgttgcc
     1021 ggcgatgaag atcgctttca cgtcgttcgg cgaagcgctc cactgcagct ggttcacggc
     1081 ctgggcgatg acgtggccgc agtattcgtc gccgccgtta gtggtcagtg agaacagcgc
     1141 ctcggacacc cggtccagct cccgcgtcaa cccgacgacc tgccggacat ggccgagccg
     1201 ggcatcgata ccggtgttgc cgtactcgta caccgccact tcgaacactg gcgtcacgcc
     1261 gttgcgcttc gccttcgaaa actcgttgac gacctgccat agctgctggc gagcctggtc
     1321 gatgagcccg tccatgctgt tgctggtgtc cagcaggatt gcgagctgga ttctcggcgg
     1381 ttggttcgca atgtgccggt ccggctccgt tgtctgtgga ttcgcccaca taagggcggg
     1441 cgcggcgagg gccagcatag ccgtgagctg aataaggcgt atcatgagtg gactcctgtc
     1501 tgagtgagcg tcgttagtca ggctacgagt ccgggtcaac gaatggcagg cacggatttg
     1561 taaaacgtcg cgggacggat ttacacggaa cttacattcg gcaagcggca gctacgacac
     1621 atggagagaa aaatgactac aaggtctaag cgtttgctgc cgatcctgct cggcaccttc
     1681 gtgtgctcag ctaacggcgc cgcggaaccg gccgactggg gtgcgataga ggtcgcgggg
     1741 aactcagttc aaccggggac aataggcagg tttccggcga tcagtcagcg cggtttcgag
     1801 gccacgtatc tcaacctgcc ggtctacgtc gcgcacggcg ccgaggcagg cccaacgctc
     1861 tgcgtgactg cggcgataca tggtgacgaa accaacagcg tggaaattgc gcggcgtgtg
     1921 tttgcagaga ccgatgccac tgggctgaaa ggcactctct atatgctgcc ttttgtcaac
     1981 gccgatggtt tccgcaaccg aaaacgcgaa atgaccgatg gccgcgacct gaaccgaact
     2041 tttcccggca gcgcgaacgg gagtgtcgct tccctcgtcg ccaacttcgt cttcacagac
     2101 ctgacatcgc ggtgcactgc attgatcgac ctgcacacag gatcgaatct ccgttcgaac
     2161 tatccgcaga ttcgcgtcga tctggagaac gaagacgcca aagaacttgc cgtgcatttc
     2221 ggtgtcggca tgatactcgg cggcggtggt ccggatggca gtttgcgcgg cgagatggtc
     2281 aaggcgggaa ttccagccat catctatgaa gcgggtgggc cgctgttgtt ccaggaagag
     2341 gaagtcgtac gtggaacgga gggcgtcagg aacgtaatgc actatctcgg catgatggaa
     2401 attccgggct ataaagcctc gcagccgaag atttacgaga agacccggtg ggtccgcgtg
     2461 ccgcgcgggc aaggtggctt cttcttcccc gaattgtcca tgggagcgaa ggtagaagaa
     2521 ggaacccgta tgggctacgt ggttgacccc gtcacggacg aacagcatgt cataacgtcg
     2581 aaggatgccg gccagatcat cggcatgacc ctgccgcagc ctgttctctc cggttacccg
     2641 ctgttccacc tcggggtgga atgaatgcac gcgccgtctg ggttgacgct gagcaggact
     2701 taacggcgca ggtgtgcaac agtctcgcga tcattggcgg ccggcaccga tggagcagtc
     2761 gccacgaggt ggattatctg ttgtagccac tgatagacct ggttcgcatg gtcgtgagct
     2821 actgcaccgg cattcgttga agacgtcgtc acgatcacga gatccagttc ggggaccagc
     2881 atgatgaact ggccgccgtg gccccacgca tgcggaacct ggtatccggc cagctcgcaa
     2941 atccaccagc cataaccgta atagcgcgct cccccatgcg gcgatctcga gcgcggctgt
     3001 atggaagcct cgacccacca ggccggcacg atttgtcgct cgccccgacg accgccgttg
     3061 aggtacagct cgccgaaggc gagcatctgc cggggcgtca gtctcatgtc attgccgccg
     3121 aagtaaattc cttgtggatc tcggggccag ggtgcgagtt cgaagcccag cggctccgtc
     3181 agcgcttcgc gggcaaactc caaagtgctg cgcccgctcg cagtcgtcag aattgccgaa
     3241 agcaggtggg tgtttcccgt gctgtactgc atctcctggc caggcgtcgt ttccagcggc
     3301 tgacgcaatg cggacactac ccagttgtca ctgagaaccc aggcgctgta gttcccgtta
     3361 ctcgtcgctc gcaatcccga gcgcatcgta agcaagtcct ccagcgtgat gctgcccttg
     3421 tcgggaccaa cctcggtcaa cagttcgccg aagtaccgag tgagggtgat ttcgacaccg
     3481 tcgaggattc tctgctcgat tgcaatgccg acaagtgccg agaggatgct tttcgatacc
     3541 gactttacat tcgccaggtc gtcaggccct cgtccgttga agtaacgctc cagcagcaac
     3601 tcgccgtgtt ggctcaccag caggctgtgc aatctcggca tggcatcgcc ctgggcgatc
     3661 gcgcgatcga gatgtgcggg cacgcttcgt tccggcacat ttgcccacgc aagttccgcc
     3721 gcctgcgatg ccgggcagaa aaccgcagac aggcatacga tcactaacgc atgcatcctt
     3781 gcggctgaca tcggtccctc gcggtcgaag ctactggaat gatacgcagc ggatcgaggg
     3841 acgtaccgtg aacgcgttca cgcaacggga tcgaagaaca gacacaaatc gcacacgacc
     3901 ggaacttcag tcggaagcgt gacctgggtc gcggcttcgc ttacggattg cctagagtcc
     3961 cggccacacg accggggcga ggaacgctac cgcgagcccg agcagcaggg tcagcggtgt
     4021 tccgacacgg acgaaatccg cgaaacggta atcgcccggt tcgtacacga gcaagttgcc
     4081 gtggtggccg atcggcgtta gaaacgcaac tacggcgccc atggccacgc tgaacaccat
     4141 ggccggcgcc gagatgtcga gcagtgccgc cataccgagc gcgaccggcc ccagcaggat
     4201 agtggtcgcc gtgtccgaga ggatctgcgt gaacagcgaa gccgtcagga agagcgccag
     4261 cagcacccag gtggtctccc acgggcccac cagctccgcc agccagccgg cgaacagcgc
     4321 ggccgtgccg gtctgctcca tggcgatgcc gagcgggatt gccccggcta tgaaaacgaa
     4381 aatccgcgtg tctaccgagt cgtacgcctg cttcagcgac aggcaaccgg tcagcaccat
     4441 gacggcggcg ccggtcatga aggcgatcgt taccggcagg acccccgtgg tcgccgcgac
     4501 gacgctgccc cccatgatca gcccggcgag ccagcccttg ccgagcttcc ggtcgcgcgc
     4561 ggagaacggc aagagcagca ggaacgacgg gttgtcggcg agcctgtcga gggccttctg
     4621 gtcgccccaa agcaccagca ggtcgccggc cttgaggact accttcgaca attccttgcg
     4681 catgaagccg cgcttgcgcc acagcccgac gacgacgacg tcatagcggg ccaggaaatc
     4741 cacgctgccg atgttgcggc cgatgagctc cgaatccggc ccgacgacgg cctgcacgag
     4801 gcgctcgctg ccgggcaggt ccttcgcctt ctcgtccggg attacctcgc cgtactgcat
     4861 gacggcgtgc agcgcaagcc cctgctgctt ctcgagcgtt gcaagctcgt ccgggctggc
     4921 ccgcacgagc agcaggtcgt ggggtgcgat ctccttccgc tgccacggcc gtgcgcgcga
     4981 ctggccgtcc cgcagccagt cgacgacctg gaaacgttcc tcgtgctcct cctggaactc
     5041 ctgcatgctc ttgcccttgt aaagcgagtc ctcgagcacc accagctcgg tgtagtagcg
     5101 ttcgagccgg aagcgatcct cctcggcggt agcgccctcc cgcgtcggca acagccagcg
     5161 cccgagcagc aggatgtagg cggtaccgat gagcgtgagg acgatgccta tcggggtgat
     5221 gtcgaaaatg cccacgtcgt gctcaccggt gcgttgcagc aggttgcgcg cgaccaggaa
     5281 cgccggcgcc gcgacgacgg tcatggtggt gccgagcgac gccgcgaacg ccatcggcat
     5341 catcagccgg gaggagggaa tattgtgtgt gcggttcagg ccaaggatca ccggcagcat
     5401 catcgccgtg atcatcaggt gatggctgaa agccgccatc agcgccacgc tcggcatgat
     5461 gacgagcacg gcgcgcgaca ggctctcgcc ggacaggcgg ccgatccagc gccccaaggc
     5521 ctccgtcagc cccgtatagc tcaaccctcc cgagagtacg aacatcgagg ccaccaggat
     5581 cgccggctcg ctcgagaatc ccgacagcgc ctcggatggc gacagcaggc cggtgccggc
     5641 gagcgccacg acgatgaaca cgccggtgag gtcgaggcgg atccactccg tcaccagcag
     5701 cacgagggcc acggcgagaa tggcgaggaa gatgatctgc tggagatcca tgcacgcccg
     5761 gctacggagg aacgacctgc tcatggtagt ggccgggggt gccggcgtca ccagatggaa
     5821 tcccggccta caaccgtcag tcctggatgt cgatattgag gcgccccgcc atcccggaat
     5881 aacggggatc cgatcgcata ccgtcgaata ccgggtccca cggcagccag gcgataagtt
     5941 gcgaccgttc cgcgaacgcg cgctccagcc aggtgaaggc ctcgtccctg ttgccgaggc
     6001 tcgcatggat cgtggcgatg tcgtaggcgg agacgtaacg ttgctgagag tcggcgacga
     6061 gcctctggat ttcggcctcg gcttccttgc gtcgtccggc caaagcgtgc gcctgaccac
     6121 gataggcggc actgcctgga gccagtgacg acgcctcgtc gaggtgttgc agggcgcggt
     6181 catactctcc gcgacgcaaa taggcgagtg ccaaataggt gcgtgtcgtg gcgagcgacg
     6241 gatcaagctc gagtgtcttc tcgaggtgct cgatggcctc gtcgtagcgc cgctggtaga
     6301 tcagcagcat gccgatcagt gcgccgtaca gcggcgaggc cggctcgagg gtctgcgcgt
     6361 gatgcatccg ttcgaggcct tcttcaaagc ggcccgagaa agaccgataa atgccgagcc
     6421 actggtgcgc cggcgcatag ttcggactta gatcgatggc gcgtgcatag gcacgctctg
     6481 cgccggacca gtcgtgctcg tactgcactt tgacgtgacc gagcgaggca tacgcttcgc
     6541 ccagctccgg cgccaattcg agcgcccggt gcacggcttg ctgcgcgcgc ggaaagacat
     6601 cgtgcggcgc cgcgatgccg aaaacgccga ggccggcata tccctccgcc aagcccacgt
     6661 acgccagggc gaagtcaggg tcgcgttcga ccgcttcgtg aaagaatccg agcgcttccc
     6721 gaagcccggc ttcgttgccg cgccgccggt ggaaacggcc gctcgcatac agctggtacg
     6781 cttcggcgtc ctcggtgtag cgcctgaacg gcggcgtctc ttcaccggtc agctccgggg
     6841 ccagcgcggc ccgtaccttc gtggcgatcg catcctgcac gctgaagata tcggtgaaat
     6901 tttcgtcgta gcggtcggcc cacagttggt ggccgtcgcc ggcgttcatg agccgtaccg
     6961 aaacgcgcag ccgctctcca tcacgttgca ggtgtccctc cagtaccgcg tcgacaccca
     7021 gctcgcggcc ggcggcaacg gcgtcctggc cgggatcggc atagcggcgg acggcgctca
     7081 gcgggctgac gaccagccgg gaattgctca ggcgcgcaat cagggactcg gccatgccga
     7141 gctccaggga ctcgttgcgg tcggtagcgg tcagcggccg gaacggcagc accgccaggg
     7201 tcgcggcggg aagatccgac actggcactg gtcgttcgcc cggccactcc gcgagcacgg
     7261 cagccagggc gagccccgcc gctgcggcca acccccacca cttgacgagg tcgaaacgat
     7321 tatttgctgt ggcaggattc ggttcgaccc catttgatcg ggcggccggg cggccgttgc
     7381 cctcggccag ccgacttacc ggcgcgacga agcgatagcc ccgccggcgc accgtcgcga
     7441 tgtagcgggg ctctccgcgt cgctccccga ataacctccg cagggtcgat atgttctggt
     7501 cgaggttgtt ttcctcgacg accacgtccg gccagatcgc ggccatcata cgtgccttgt
     7561 cgagcaactc ccccgggtgt tcgacgagga aaagcagtgt ctcgaacacg cgcggcgtca
     7621 gcgggatcca ccgatcctcg gcttcggaac gaagtgcctg gcgggcgacg tggagctgga
     7681 actcgccaaa cgtgtagatg ggggtgccac cgggcacttc catgacgaag ccttcgccgg
     7741 ttcagaaata ttcagaaaac gttcagaggc cgctaaggat aggccgggtc cgcgactgca
     7801 ttctagcagg tgaagcgctg cagccgcggc gcaaaccgga ggtcacaagt atgaacgggg
     7861 caatgaagac tttggctgga aatgcgttgt ttgccgtgct ggtggcaggg atgctggcgg
     7921 tacagccggc caaaggtgag ggacaggagg acgtcctgca gcagcacgcc agcacagatt
     7981 gggacaagct gtggtgggga aaagatccca ttgagggagt ctggaatgta cgcgtggtca
     8041 tcacggactg tgcttcgggc ctcccgctcg gaccgccccc attcgacgca atggcgatgt
     8101 tcaacgccaa cggcaccttc caggatacca actccaacag cccggccctg caggtcaggt
     8161 ccgaagcatt gggctactgg aaatacgtca ggggaaacaa gtaccggttc gccttcaagg
     8221 cgtttcactt cgacctgtcg gggatgtacg tcggctacaa catcgtgagg cacgacgtgg
     8281 tgctggccga caatggcaga agctacatct caaccggcac cgtggagttc tttaatccag
     8341 acggcactac gcggccgccg gtgatgggct gttccgaagc gaaagcaacg cgcttcaaat
     8401 aggcgggcaa aaattccggt acgaaaaaat gcggcaggag cgacgcaata tctgcgattc
     8461 gctcctgcct catgttcaac cctgcacgcc gacgtcgagc ccggagtcat aagcccatag
     8521 actgacgctg cgggcggcgg aaaacgccag accggaagag ctcgggcggc atcagacccg
     8581 cgccgcccgg atctcgccgc ctaggacgtc agagccgtct gcactggtct tccttgttgc
     8641 catcgaccac gttgcggccg ccaaccggcg gcggcgagtt cgctttgcac tgcagattgc
     8701 cgtcgatcgt attgcggcga atctcgactc cgcccacgtt ctggaaggcc tgcacgttgc
     8761 cgccgacaac gttgcgcagc acgcgcagca tcgcggagtt cgattcgtac tggatgtcag
     8821 cgtcgacccg gctgtcgctc acggtggcgc cgccgccctg cttcacctgc accgaaccgc
     8881 cgacgcgcga accttcgagc acgcgcacgt tcttggcatt ctcggcctgg acgttgccga
     8941 tcacgaccac atcctccgcg cgaagcgtcg cgttcgactc gaccttgatg gtgcccttta
     9001 cccgcgttcc tttcagcacg cagttcgcgt tatccggcac gcgcaggttg tcgaccgtgg
     9061 tcgctccgat cgttccgcgg catacgcgtt cctcggcagc tgcgggagca gcggccatga
     9121 ccaaaagcgg cgtcgccgca gtcagcgcga tccaggtatt gcggtactgg cttttcattc
     9181 ggttgttctc cactgagtga ttgataaacg tctgccagac gagatgcagt tcgcgtgcca
     9241 accgatcccg aggccggcac gactggtgga agccttgaaa cttatggcat taagttcggg
     9301 acaggctggc acggcggcca cacgccgggg tgccggcgtc cggaatctgg acggcccgtc
     9361 tagttccgag acggtttcag gccgtagtcc ttgagtttct ggtagagggt gctgcgcgga
     9421 atgccgaggc gccgagccgc gtccgccacc ttgcccttcg tcgcgtcgag cgcgcgctcg
     9481 atgtgccgcc gctccagctc ctcgagcgtc agcatcgagt cgtcctgcgc cggcatcgcg
     9541 tgcgccgtac cgaagcgcag gtccttttca tcgatcgcat ccccggatcc caggagaagc
     9601 gcgcgttcca ggacatttct gagctcacgc acattgccgg gccagcgata cgcgagcaac
     9661 gccttctcag cggcggcggt gaatgcgggc gcggtccggc cgagatccgc cgccagttgc
     9721 ccgatgagcg cctttgcgag tatgacgacg tctgcatccc gctcacgcaa cggtggcacg
     9781 acgagtggta ttgcgctgat gcgaaagtaa aggtcgctcc tgaatcggcc gtcgcgcatc
     9841 agcgcgtcga ggtccgcatg ggtcgccgcg atgaggcgag tgtccacgaa ccgctcgcgc
     9901 acgtcgccga gccgcctgaa tcgtttttcc tcgaggacct tcaggagctt cggctgcacc
     9961 tgcgaatcga cgtcgccgat ctcgtcgaga aagacggtgc ctccgtgggc gatctcgagc
    10021 aggccctgct tgcgtgtcac cgcgcccgtg aaggcccctt tctcgtggcc gaacagctcg
    10081 gattccagaa actcgcgcgt caggccagca cagttgaggt cgacgaatgc ctctctcgag
    10141 cgcggaccgt gtcgatgcaa ccaggctgcg acgacgccct tgccggttcc cgtttcgccg
    10201 cagatcagga tagggctctc ggcatcgagg acctttcggg cgtggatctc cagttcgcga
    10261 atcgccgcgc tcgtgccgag gaatggatcg atggctgcgc gccgctcgcg ggccgagccc
    10321 acgagatgcg cgcggtgcgc acggcgcgcg tcgaggagcc gccgcaggat gacctggaat
    10381 gccggcagct cgattggctt ggtcaggaac tgctcggcac cctccttgat cgcctgaacc
    10441 gcgaggtcga tcgagccgtg ggcggtcagc acgatcagtg gcacggtcgc gtcgatttcg
    10501 cgcaggcgcg gcaacagcgt caatgcgtcg ccgtcctgca gcttgtagtc gatgacggcc
    10561 gcatcggggc tcgtggacag gaagacctcg agcgcctgtt ggcagctccc ggcttcgtgc
    10621 acttcgtagc cttgagtctc gagatattcc cgcagcccga agcgcaccgc cggctcgtcg
    10681 tcgatcacga gaatccgttt cttcatgcct gcgccaccgg cagcctgatc gtcacgaccg
    10741 ctccgccctc ctcgtggttc cccgccaata cctcgccgcc gtgctcacta acgatgcgct
    10801 gcaccagcgc gaggccgagg cccgtgccac cgtgccggcg cgtgaagaac ggctcgaaca
    10861 cgtgcggcag gtccgcggcc gagaaaccgg gcccccggtc gcttaccgca cattcgatcc
    10921 aactgccccc tcggtgctcg atgcgccgcg tctcgatcgt tacgatcgat ccctgcggcg
    10981 catgccgcaa cgcgttgtcg acaagattgt gcagcgcctg ccccagccgc cccggatcca
    11041 ggcgaaccgg cggtgggtcc gggtcgagcc ggacctcgat cgtggctcct gtctcgcggg
    11101 cgagagtgcc gcaggactcc aacgcccgcg ccacgattgc gtcgatggag ccagtgctga
    11161 attcgctcgc agtcggccgt ccgtatgcga gcaggtccgc catcaggctg ccgagccggc
    11221 tgacctggcc ctggagattc tcgatgaaac ggctgtattc cgagcgctcg ccgagacggg
    11281 cggtcagggc atcgagcgtc gacgagatcg cgaacagggg attacgcacc tcgtgcgcga
    11341 cacctgcgac cagcgcgccc atggttgaca tcgtctcgct gcgccgcaac tcggcctgga
    11401 gactctcgcg ctcatgctct gcgcgctcac gctccgccat ctcctgcttg agccgatcgc
    11461 gcgagacggt cgtggcctcg agctggtcca ccatagcgtt gtattgtcgg gcgagatcgc
    11521 cgaactcgtc gttcgatgca ggtggaatgc ggtatgacaa gtcgccgccc gtcacggcga
    11581 gcgtgccgcg atagagcttg ccgagtggct tcctgatcac ctggatgagc ataaagccaa
    11641 tgacgccggc cgccagcagg aacatcggca cgagcaattt catcgcgaca agcacctcgt
    11701 tggcagcggc cttggcctgc gccttcggct cgtccattcc ccctaccgcg aggacctgga
    11761 tgttttcatc cagcagatca tccatggatt cgcgcagccc gatgagccga tttcggcctt
    11821 cgaatatctg gtcctcgaga gccaggactt cgcggacatg cacggccacg cgaccaacaa
    11881 gcttgcgcag cgtgggctcc agttgcctgt cttcgccggc cagatcgaag ccgagaaaac
    11941 tgtgaagagc gctctccatg acagtgagct tggccagaat cgtgcgccgc gctgacggcg
    12001 tcggaccacg atggtagttc gccgcccatt ggccgacttc cgccgtctcg gcctcgaaat
    12061 tggcgctggc gacggccgcg ccgatgccga cgcggcgcaa ctcggcctcg cggaacagcc
    12121 ggggctgcag gcggttgtcg aggacgtagt cgatctcttc catgtcctcg gtgatcgcgg
    12181 cgtagagctc ttcctgccgg tccgcgagat ccatcagctc gcccgcgagg gtcgcaaacc
    12241 ggctgtgctg gtgatagatg cgacgtccaa gctcgcgttc ccggtctgtc atcacaagtt
    12301 cgagatacgt cgcatgatag ctggtgaagt ctccgacgtc gtcttccgcc tcggtccggt
    12361 cttcgggccg ccggccggcc aggtacgtaa gtatgtgcag cgccgcaccg ttgacgttca
    12421 cttccatccc gtatgcggca gcaacgatcg gggcctgcac ggccgcgacc tgctcgtgcg
    12481 cgacgcccac gcgctcgagg ccgcggtaga tgctcagcat ggaagccatg ccgaccgcca
    12541 tgatgagcgc tagccccagc gcgacctttc gggtgacgct gaagcgccag tttccgtgcg
    12601 tcatgggaat cactccgtcg cggccgcagc cgggcagtct gtccgtcgtc ggattatcct
    12661 gcgccttcgc tgcctcgtcg cctgcggcga cgcctgtcgc gcgacttcgt cgcgctcgga
    12721 tcgaacctgt gacccccgcg ttcggagcgc gccttttttt tggatactaa ctaactgaaa
    12781 tatatacaaa atctggtggg ccgcccattg caattccagc actacgcagg actttgcagc
    12841 gcgcgttcgc acgattttca cacattggcg ggtacgcctg cctaccgaaa gggatcgtca
    12901 cggcgctcgc taatctcgtc cgggcgatta attgctatca cagcaacctg gtcgaagggc
    12961 acaacacgca cccgatcgat atcgagcggg caccgaagaa ggattacagc gccgatgctg
    13021 agcagcgaaa cctgcaactc gaagccgaag cgcatatcac cgtccagcag tggattgatg
    13081 aaggcgcact cagaggccgt gcaacttctg tcgattcaat ccttggaaat ccaccaacat
    13141 tgaaatccac cgacgctttt gcgaaatgct gcgcgcgaag ctgctgctcg tagagcatcc
    13201 ggcaacaaag aaaaggagcg tcgtgccggg tcagctacgt cagcacgatg ttgaggtcaa
    13261 gcatctcgca aactgtgatc tgccacgcca ttactatctt gacggcagag gagccgctat
    13321 cgcggacgaa gtcaaggcga atccagcaaa ctgcgcagta tgctctgaca acccagttgt
    13381 gacttcgcag ccccggctgc aatggacctt atcggcgaaa gaagtccacg ctggccgagc
    13441 gctcgcgcac tcgggtcgcg ggcgctcggt cgcggatcag gtcgcgcagc cacgcgagcg
    13501 gcgggttacc gctcaggccg gacaggaatg cgctaacgac cgtcaaatag aagaacagca
    13561 agaacaggaa tccggcttca accatctcac cctgggcatt ggtaaacccg accaccccac
    13621 aggcaatcat caaaaagaag aacgtcgccg tccatttcaa catcgtgctc tcctcaggat
    13681 tgttggaacg ccgcattcct cgcccccggt tttaatgcct acgactttgc catgcatatt
    13741 gcgtgccagt ggagcatggc tattgaacgg accggcaaca cccctgctgc aggccaaaat
    13801 tgcacaccgg tgtaaatttt atccttccac cagcagcgct cgggactcta tttcatcggc
    13861 ctgccggcag cctcggcagc agagccacag gcatcgtgga ggccgtgcgg agccgagtgc
    13921 aggaggtgac cccgggtgat atcgcgtggt ctacaccggc ggcgcgcctg gcgcatatgc
    13981 ggaacggcgg aacatcgcgg ccgaacacac ctgataccga ttccgcgcgg catcggggac
    14041 aagagtctgg cgctgcggcg ggcgactgcg ggtcgtcatc gccgcatcgc ggatccggcg
    14101 gttatcgagc ggattcttga acacctcggc cgggacgcag agtccgtcga tccgacgcat
    14161 ccgagccgcg cgccgcccca gggcagtttg tcgctctaac cgccactcca ccgcccatgc
    14221 gactttggca ttacagagcg ttcaggaccg gcacgcgcac tgttgctttc gtaccgggga
    14281 aagttcatgg acggtcggtt tggtgtggaa ccgttcgttg cagcgtgacg tgattgagaa
    14341 cttccgattt cacaacctca gacatacgtg ggagtctgcc cgagttgatg gaactcggcg
    14401 gttggaggtc atacgagatg gtgcttcggt acgcgcactt ggcgccggag catttatcga
    14461 atggagaagg caagcgaagg gggtatcgaa atcgcaagaa ccaacgctat gatttcgcta
    14521 cgcgtaaaaa aaactcacca cgagtcaggc ctatgtgtcg tgctcgaaga tattcaaact
    14581 cccgaccgcc tgattcgtag cgaggttttc ccgaggggca caagctgaat tacctattat
    14641 caagtggcgt gacgcccgtt gcagattcag tacttatcac aacgatgcag agccgattca
    14701 cacaaatctc atacagccgg cactgcgacg tgatgctcgg cgtgtccgac gagttgctgt
    14761 aatcctttcc gctcacgagc gcgaacgctg gcggacttga aatcgccggc gcgaggcgca
    14821 caatgggccg cgaccgggag acgaaatgtc atacctctgg cctcgtaagc cgattgttct
    14881 gccgctgctc agcgccgcgc tcttcggcgg ctgcggcgga acaccttccg gacccacaac
    14941 cgccgtctcg ccggcgctcg gcgataattt tgccggcgtc gtcaccgagc cgttcggcgt
    15001 attcgccggc gtcgagtatg tccggcacac gggcgtcttc gagggcgaaa gcacattggg
    15061 ggcgtttcgc gtcccgttcg aggtcgttgc gccggccgac ccggacctcg gcgaaaacac
    15121 gttactgttt gagccgccgc atagcgggtt cggaacagtg ggccgcaatc tcgttctcgg
    15181 tccggatttg attttcggcc gcggattgcg cttcgcctcg gtcggcgtcg gcaatttaag
    15241 tgacttgcac atcctggacc cgtcaattcc cgaccttctg ctcgccggag cggcctttgt
    15301 gccggctgac ccggagaacc ttccggccgg atccatcatc gatgaggaga tcatcgcgct
    15361 gttcctcgcc gcgctcgaat ccgacgagga tatggccgcc atgctcgggt cgatcgaccg
    15421 gcgctatgcc tatggtattt cgcagtccgc cgccgcaatt agcgaattgc tgtttgaccc
    15481 aaccggcctg gggcaagaag tctttgacct gtcattcctg cacgcggcgc attggcggcc
    15541 tgtgttcgag acgcgtggag cgtttcataa tctgcccgat ggcagcccgg tccccgtcgc
    15601 tttcgacctg ccgacgggcg tcggccgcgt gatgatcgtg aacagtgaag gcgacgcgtt
    15661 ggtcagcgat tcggagtttt atcagtgtgc gatacaccgc gccgattttc ggctgtatga
    15721 agtctctggc gcggcgcacg tgccgacgtc ggacgccgtt cctctcggga acaatccact
//
LOCUS       DASUOM010000567         4328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1039756,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000567 DASUOM010000000
VERSION     DASUOM010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039756"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFY27_11850"
     CDS             <1..899
                     /locus_tag="VFY27_11850"
                     /inference="COORDINATES: protein motif:HMM:NF017495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HEX5766255.1"
                     /translation="GPRVLAVFFCTTLSLFTAFFLTGSIFRAWLPADAWQPLAALSGS
                     WAGGTANMIAVKEAIGMPDSLLAMSLLTDALCYSLWVAILFSVARLAPAFNRFTRAQS
                     SGDLVVEEPKATGPTTPDSVLVWLSLGLLAAAVSSWLASFLPTSNMISATTWTILLAT
                     GIGLVAGQTPLARYPGAASISGAMLIALVAVLASQSNFQGIGEAPLFILCGVLIIAIH
                     AGLMSLAARVFHFDMYLCGISSLAHIGGVAAAPLLAATYSRSLVPVGVLLALLGYVIG
                     TGYGLVVAHVLSGIYGAEVAAP"
     gene            896..2326
                     /locus_tag="VFY27_11855"
     CDS             896..2326
                     /locus_tag="VFY27_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5766256.1"
                     /translation="MKRAHFMILVAGLLLSACLRALPAFAETAAQPAHGVIGVEEVHL
                     DPDFWVARLEDPDRVVMMRQAIEEQNAKLFELDATMHDLRDLPATLPRDYVFALIEDL
                     ADPPSGTMFDESGKSVSMRTVKAIAKNRNLYDIPAEQATRYGLVVHRADLRTFPTSLR
                     VFRSAGNTDIDRFQESALFPGTPVVIAHESGDGEWWFVVSPRYAAWIEKQHVAEGTAA
                     EVLGYVDKAPYRVITGATVDTVFNPEHPEVSELQLDMGVRVPVLAGEANAPVNGQHPY
                     SAHVIELPVRAEEGQLRLLPALLQRNSDTDADYLPMTRANIIRQGFKFLGERYGWGHS
                     YNGRDCSGFVSEVYRSMGALMPRNTSAQSVSPALQARLFGVGDGQKARMEAVRSLNVG
                     DLVFIPGHVMLVIGNIDGQPYVIHDTTGISYRLDDGDMARVSLNAVSVTPLLPLRVNE
                     TETYVDRMTSIVKIVPDISKEASPSL"
     gene            2323..3417
                     /locus_tag="VFY27_11860"
     CDS             2323..3417
                     /locus_tag="VFY27_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008218716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5766257.1"
                     /translation="MKISDIKFGTLRVPLKTPFKTALRTVDKIEDIVVMISTDCGHVG
                     YGEAPATAVITGDTHGSIVEAIRHYIWPRVKGQDVANLNHLTQLIQGAMEKNSSAKAA
                     VEIAVYDLWGQLYDAPLYRLLGGGDPLITTDITISVDYIDKMVADSIAAVERGFESLK
                     IKVGKDIGVDIERVKAVHAAVEGRALLRLDANQGWTAKQAVYALQSLEDAGVRLELVE
                     QPVKAQDLNGMKYVTERVHTPIMADESVFGPTEVVELIRMRAADIINIKLMKTGGISN
                     AIRIADIAAMYGVECMIGCMLETGISVAAAVHVAVAKSDAITKIDLDGPSLCTFNPID
                     GGVIFNDSEISVTGAAGLGIREIRGLEPLT"
     gene            3443..4291
                     /locus_tag="VFY27_11865"
     CDS             3443..4291
                     /locus_tag="VFY27_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX5766258.1"
                     /translation="MSPLLKIRAERDQMSAIERRIADYILDEPHLLRDYSSQQLANAL
                     KISQSSVVKFSQKLGFKGYPDLKYSIGESLARGDGGDRESNVDQRPEDPHSALAESLW
                     YSKTQAEQETRLINPSTKIDAIADAIGEAGKVFIIGLGEDGIPSRWFAMRLSLLDILT
                     VHHLDPVLMAASIATTAPGDILLVFSEHGQQPALCQIGRQFRERHGKVVSVTRHTANP
                     LRAQSDQTLLVSAHDEREHIQPLLYQSALRHLLDMLFVILTENGDGRLDRYAANLERI
                     QNLFEP"
BASE COUNT          816 a         1359 c         1337 g          816 t
ORIGIN      
        1 tggggccgcg cgtactggcg gtttttttct gtacaacgct gagcctgttc actgcctttt
       61 ttcttaccgg cagcattttc cgcgcctggc tccccgcaga tgcgtggcaa ccgctggcgg
      121 cgttgagcgg cagctgggca ggcggtacgg cgaacatgat cgcggtcaag gaagcgatcg
      181 gcatgcctga ttccctgctg gccatgtcgc tgctgaccga tgccctctgc tactcgttgt
      241 gggttgccat tctcttctcc gtcgcgaggc tcgcgcctgc gttcaaccgc ttcacccgcg
      301 cgcaatcgag cggggacctg gtcgttgaag agccgaaggc aacgggaccg acgaccccgg
      361 acagcgtgct cgtctggctc agcctgggtc tcctcgctgc cgcagtgtcg tcctggcttg
      421 catcgttcct gccgacatcg aacatgattt ccgcaaccac ctggaccatc ctgctcgcaa
      481 ccggcatcgg cctggtggcc gggcagacgc cgctggcgcg ataccctggc gcggcgtcga
      541 tatccggcgc aatgctcatc gccctggttg cggtactggc gtcgcaaagc aacttccagg
      601 ggatcggtga agcgccgctt ttcatactgt gcggcgttct catcatcgcc attcatgccg
      661 gcctgatgtc gctcgcggca cgcgtgtttc atttcgacat gtacctgtgc ggtatatcct
      721 ccctcgcgca tattggcggc gtggcggccg cgccgctgct agcggcgact tactcccgct
      781 cgctggtacc ggtcggagta ctgctcgcgc tgcttggcta cgtcatcggc accgggtatg
      841 gcctcgtagt cgcgcacgtc ctgtcgggaa tctacggagc ggaggtggcc gctccatgaa
      901 acgcgcccat ttcatgatac tcgtcgctgg cttgttgctg tcagcgtgtc tccgggcgtt
      961 gcccgcgttc gcagagacgg cggcacaacc cgcgcacggg gttatcgggg tggaagaggt
     1021 gcatctcgat ccggacttct gggtagcgcg gctcgaggac cccgaccggg tcgtgatgat
     1081 gcggcaggcg atcgaggagc agaacgcaaa gctattcgaa ctcgatgcga cgatgcacga
     1141 cctgcgggac cttccggcga cacttccgcg cgattacgta tttgctctca tcgaggatct
     1201 cgcggatcct ccttcaggca cgatgttcga cgagagcgga aagtccgtgt cgatgcgaac
     1261 agtcaaagca atcgcgaaga accgcaacct ctacgacatc ccggcagagc aggcgactcg
     1321 ttatgggctg gtcgtgcatc gcgcggacct gcgtaccttc ccgaccagct tgcgcgtctt
     1381 ccgctccgcc ggaaatacgg acattgaccg cttccaggaa tccgctttgt ttcccggcac
     1441 gccggtcgtc atcgcacacg agagcggcga cggcgagtgg tggttcgtgg tctccccgcg
     1501 ttatgccgcc tggatcgaaa agcagcacgt tgccgaaggc acggccgcgg aggtgcttgg
     1561 ctatgtcgac aaggcgccgt atcgcgtcat cacaggcgcc actgtcgata ccgttttcaa
     1621 tcccgaacat ccggaggttt cggagctgca gctcgacatg ggcgtgcgcg taccggtgtt
     1681 agccggggaa gccaatgcgc cggtcaacgg gcagcacccc tacagcgccc acgtgatcga
     1741 gctgccggtg cgcgcggaag aaggccagtt gcgcctgctg cccgcgctgc tgcagaggaa
     1801 ctccgacacc gatgctgact acctgccgat gaccagggcg aacatcattc gccagggttt
     1861 caaattcctg ggcgaacgct atggctgggg ccattcctac aacgggcgcg attgctcggg
     1921 cttcgtctcc gaagtctacc ggagcatggg ggcgctgatg ccgcgtaaca cctctgccca
     1981 gagcgtgagc cccgcattgc aggcgcggct gttcggcgtc ggcgacgggc agaaagcgcg
     2041 catggaggcc gtccgttccc tgaacgtcgg cgacctcgtg ttcattcccg gtcacgttat
     2101 gctggtgatc ggcaacatcg atgggcagcc atacgtcatc cacgacacga ccggcatcag
     2161 ctatcgcctg gatgacggcg acatggcgcg agtgagcctg aacgccgtct ccgttactcc
     2221 cctcctgccg ttgcgcgtca acgaaaccga aacctacgtc gaccgcatga ccagtatcgt
     2281 caaaatcgtt cccgacattt ccaaggaagc ttcgccttct ctatgaaaat ctccgacatc
     2341 aagttcggta ccctgagggt cccgctaaag acgccgttca agactgcgct gcgcaccgtc
     2401 gacaagatcg aggacatcgt ggtcatgatc agcaccgact gcggtcacgt cggttacggc
     2461 gaagcgcccg cgacggcggt cattaccggc gacactcacg gctctatcgt cgaagccatc
     2521 cggcactaca tctggccgcg cgtcaagggg caggacgtgg cgaacctgaa ccacctgacg
     2581 cagctcatcc agggcgccat ggagaagaac tcgagcgcca aggcggcggt cgagatcgcc
     2641 gtctatgacc tctggggcca gttgtacgat gctccgctct accggttgct cggcggcggc
     2701 gacccgctca tcacgaccga catcacgatc agcgtcgact acatcgacaa gatggttgcc
     2761 gactccattg cggcggtgga gcggggcttc gaatcgctga agatcaaggt cggcaaggac
     2821 atcggagtcg acatcgaaag ggtgaaggcg gtgcatgccg cggtggaggg cagggcgctg
     2881 ctgcggctcg atgcgaacca gggatggacg gcgaagcagg ccgtctacgc gctgcagtcc
     2941 ctcgaagatg cgggcgtgcg cctggaactg gtcgagcaac cggtcaaggc gcaggatctg
     3001 aacgggatga agtacgtcac cgaacgggtg cacacaccaa tcatggccga cgagagcgtc
     3061 ttcggtccga cggaggtggt cgaactgatc cgcatgcggg cggcggacat catcaatatc
     3121 aagctcatga agactggcgg catctcgaac gcgatccgga tcgcggacat cgccgcaatg
     3181 tacggagtcg aatgcatgat aggctgcatg ctggagacag gcatcagcgt cgccgccgcg
     3241 gtccatgtcg cggtcgcgaa atctgacgct ataacaaaaa tagacctgga cggaccatcc
     3301 ctttgcacct tcaacccgat agacggcggg gtgatcttca acgattcgga gatcagcgtt
     3361 accggcgctg cgggcttggg tatacgggag attcgcgggc ttgaaccact aacctgaaaa
     3421 gctccactga tgatgacgga cgatgtcacc gttgctcaaa atccgcgctg aacgggacca
     3481 gatgtcggcg atcgaacgcc gcatcgcgga ttacattctg gatgagccgc acctgttgcg
     3541 cgactattca tcgcagcagc tcgcgaacgc actgaagata agccagtcga gcgtcgtcaa
     3601 gttcagccag aagctcggct tcaagggtta cccggatctc aagtactcga tcggcgagtc
     3661 gctggcgcgc ggcgacggcg gggatcgcga gagcaacgtc gatcagcgtc ccgaagaccc
     3721 gcattcggcg ctggccgaga gcctctggta cagcaagacc caggctgagc aggaaacgcg
     3781 gctgatcaat ccttcaacga agatcgatgc gatcgccgac gcaatcgggg aggctggcaa
     3841 agtcttcatc attggtctcg gcgaagatgg catcccatca cggtggttcg ccatgcgtct
     3901 ttcgttgcta gacatcctga cggtgcatca tctcgacccc gtgctgatgg ctgcgagcat
     3961 cgccacgacg gcacccgggg atatcctgct ggtgttctcg gaacatggcc agcagcctgc
     4021 gctgtgccag atcggccgcc agttccgcga gcggcacggc aaggtcgtat cggtcacccg
     4081 ccacaccgcg aacccgctgc gcgcccagtc ggaccagacg ctgctggttt ccgcgcacga
     4141 tgagcgcgag cacatccagc cgctcttgta ccagtcggca ttgcgccatc tcctcgacat
     4201 gctgttcgtc atcctgaccg agaacggcga cggccgcctg gaccgctacg ccgccaacct
     4261 cgaacgcatc cagaacctgt tcgaaccctg agcgctttca ataggccgct gtgggagccc
     4321 gccgtgcg
//
LOCUS       DASUOM010000568         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1278054,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000568 DASUOM010000000
VERSION     DASUOM010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278054"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFY27_11870"
     CDS             complement(<1..602)
                     /locus_tag="VFY27_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766259.1"
                     /translation="MAGLLARERIIAGPGFNRWLVPPASVAIHLCIGSVYAWSIYNPA
                     LVRTEGVVTSAAGDWSLSSVVWIFTVAIVFLGLSAAFAGKWLEEVGPRKVGVVAACLW
                     GGGYLVGGLGILIHELWLVYLGYGVIGGCGLGLGYVSPVSTLIRWFPDRRGMATGMAI
                     MGFGGGAIIGAPAKEFLLKFFYRAPEYLGARDAVELVTEGG"
     gene            complement(657..1481)
                     /locus_tag="VFY27_11875"
     CDS             complement(657..1481)
                     /locus_tag="VFY27_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766260.1"
                     /translation="MPQNRFCYLAYDGTVHPVRTGRLAACSLLVGMLFSGSAIAEPTD
                     ITIRVVARGAMYVGDLVEGAQVTLTDAGSGEVLAQGVTEGAAGDPERIMEAARKRGAP
                     MSAPGDAQFSATIDIDEPRYLQVTAFGPLQRRESATRTTMTQWVVPGKHLTGGDGWVL
                     ELAGFFVQGDLESSFVSLEQASEGVLVEAEVALMCGCPVKPGFFWDADNYEVTALVKR
                     GEAEVGRYPLRYAGSASDFAGSIAVELPGLYDITVYAHDPASGNTGVDRLELTVSE"
     gene            1636..2562
                     /locus_tag="VFY27_11880"
     CDS             1636..2562
                     /locus_tag="VFY27_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HEX5766261.1"
                     /translation="MLFSLFTWLFVLQIAGTPRIAADTPAPAQLREVADGVFVRPGQT
                     AVVFEAENVANTGVVIGDRCVAVIDTGGSRSEGDALAAAIGSLTDVPVCFVINTHVHP
                     DHMLGNEAFSEDQVRFIGHQKLPRAMALRGETYLQRAAEYGGAARADVQMVVPEQTVS
                     DEVQLDLGHRVLVLRAHATAHTDNDLTVRDEQTGTLFAGDLVFLEHLPVLDGSINGWL
                     EELGRATEQDFARVVPGHGPVEAGWPEAAGPTIGYLEGLREATRAFIAEGGDLGLAQE
                     VIAIAQPGGWQLIEQYHKRNVAAAFAELEWED"
     gene            2568..>3252
                     /locus_tag="VFY27_11885"
     CDS             2568..>3252
                     /locus_tag="VFY27_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEX5766262.1"
                     /translation="MNTRLECYAVVSYLALGVLAAGAVQATTVEEDMAWTGGLRSAYF
                     ADREILTDDLITLEAPQRAENAAIVPVSIKAAIPQTEERYIRTIWLFVDKNPGPLVGK
                     FHFTPASGRADLGLRLRVDAYSPVRAIAETSDGKLHMSRRFVKASGGCSAPASSDADA
                     AREQLGRMKFLVRHEEAEKNASEVEGSPVTLAQLMIRHPNTSGLQKDQVTHLYAPAEF
                     IKQVRVSFEG"
BASE COUNT          637 a         1050 c         1014 g          551 t
ORIGIN      
        1 ccgccctccg tcaccagttc taccgcgtcc cgggcaccga gatattccgg cgcacggtaa
       61 aaaaacttca acagaaactc cttcgcgggc gcgccgatga tcgccccgcc gccgaagccc
      121 atgatggcca tgcccgtggc catgccgcgc cggtcgggaa accagcgaat cagcgtgctg
      181 accggagata cgtagcccag gccaaggcca caaccaccaa tgacaccgta accgagatat
      241 accagccaca actcatggat caggatgccc agaccgccga ccaggtagcc gccaccccag
      301 aggcatgccg caaccacacc gaccttgcgc ggcccgacct cctccagcca cttgccggcg
      361 aatgccgccg acaggcccag gaaaacgatc gccacggtga atatccacac gacgctgctg
      421 agcgaccaat cgcccgcagc agaggtcact acgccttctg tccggaccaa tgccgggtta
      481 tagatgctcc aggcatagac agagccgatg cataaatgga tggccacaga cgcggggggg
      541 accagccagc ggttgaatcc cggcccggcg ataatccgct cccttgcgag caatcctgcc
      601 atcagcgttt tcccctgctt tccatctgca cgaccttgcc gcccgaccgt tttctcttat
      661 tcgcttacgg ttagctcgag acgatcaacg ccggtgttgc ccgaggccgg gtcgtgggcg
      721 tagaccgtga tgtcgtacag gccaggcagc tcaacagcga tactgccggc aaaatcgctc
      781 gcgctgcccg cgtatcggag cgggtagcgt ccgacctccg cctcgccccg cttgaccagc
      841 gcggtcactt cgtagttgtc ggcgtcccag aaaaagccgg gcttgaccgg gcagccacac
      901 atcagcgcga cctcggcttc cactaaaaca ccctcgctcg cctgctcgag actgacgaaa
      961 ctggattcaa gatcgccctg cacgaagaat cccgccagtt ccagaaccca tccgtcgccg
     1021 cccgtaaggt gctttcccgg cacgacccat tgggtcatcg tcgtcctggt tgcgctctcg
     1081 cgccgctgta gcggcccgaa cgcggtcacc tgcaggtagc ggggctcgtc aatgtcgata
     1141 gtcgcgctga actgcgcatc cccgggagcg gacattggcg ccccacgctt gcgcgcggcc
     1201 tccatgatcc gctcaggatc gccggcggcg ccttccgtca ccccctgtgc cagtacttcg
     1261 ccgctccctg catcggtcag ggtcacctgt gcgccttcca ccaggtcgcc aacgtacatg
     1321 gcgccccgag cgacgacgcg tatggtgatg tcggtgggtt ccgcgatcgc gctgccggaa
     1381 aacaacatgc cgaccaggag agaacaggcc gccaaccggc cggtccggac cggatggacg
     1441 gtgccgtcgt acgccaggta gcaaaagcgg ttctgcggca ttgtttttcc gcccatgggg
     1501 ttcgcgtcgc tcttttggcc gaatcgcgtt ttcgatcgag tatagcggtc gccgggacca
     1561 ggtgcaggcg ttatgatgcc tgcaggcatc ccgcgcacga gcgaccgtgg tccatccaag
     1621 gcagccgcac gactcatgtt gttttccctt tttacgtggc ttttcgtact gcagattgcc
     1681 ggtacccccc ggattgcagc cgataccccg gctcctgctc aactccgcga ggtggctgat
     1741 ggcgtgttcg tgcgaccggg gcaaaccgca gttgtgttcg aagcggaaaa cgtggcgaac
     1801 accggtgtcg tcatcggcga tcgctgcgtt gcggtgattg acaccggtgg aagcagatcg
     1861 gagggcgatg cgctcgccgc ggcgataggg tccttgacgg acgtgccggt gtgcttcgtt
     1921 atcaatacgc acgtgcaccc cgaccacatg ctgggaaacg aggcgttcag cgaggaccag
     1981 gtccggttca tcggccacca gaagttgccg agagcgatgg cgctgcgcgg ggagacctac
     2041 ctgcagcgcg ccgcggaata cggcggggca gcgagggcgg acgtgcagat ggtcgtgccg
     2101 gaacagacgg tcagcgacga ggtccaactt gacctcgggc atcgggtgct ggtgctgcgc
     2161 gcccatgcca cagcgcacac ggacaacgac ctgacggtgc gcgatgaaca gacgggcaca
     2221 ttgttcgccg gtgacctggt tttcctcgag cacctgcccg tgctcgatgg aagtatcaat
     2281 ggctggctcg aagaactcgg ccgggccact gaacaggatt tcgcacgcgt agtgcccggc
     2341 cacgggcccg ttgaagcggg gtggccggag gccgcgggac ctacgatcgg gtacctggaa
     2401 ggactgcgcg aggcaacgcg cgcgttcata gccgagggtg gcgacctagg cttggcgcag
     2461 gaagttatcg cgatagcaca acccggaggc tggcagttga tcgagcagta tcacaagcgc
     2521 aatgttgcag ctgccttcgc ggaactggaa tgggaggatt aaccgacatg aacacacgac
     2581 tggagtgtta cgcagtggtc tcctatctcg cgctcggcgt actcgccgcg ggcgcggtgc
     2641 aggcgacgac ggtcgaggaa gacatggcct ggaccggcgg gttgcgaagc gcttatttcg
     2701 ccgaccgtga aatcctgacc gacgacctga taaccctgga ggcgccacag cgtgcagaga
     2761 acgcggcaat cgtgccggtt tccatcaagg cagccatccc acagaccgag gaacgctaca
     2821 tccgcacgat ctggctgttc gtcgacaaga atccggggcc tctggtcggc aagttccact
     2881 tcactccggc aagcgggcga gccgacctcg gactgcgact gcgggtcgat gcatacagcc
     2941 ctgtacgcgc gatcgcggag accagcgacg gaaagctgca catgagccgt cgcttcgtca
     3001 aggcgagcgg cggatgctcg gcgccagcgt cgtccgacgc cgacgcggcg cgggaacagc
     3061 ttggcaggat gaagttcctc gttcgtcatg aggaagctga aaagaatgcc agcgaggtcg
     3121 agggaagccc cgtcacgctc gcgcagctga tgatccgtca cccgaatacc agcggcttgc
     3181 agaaagacca ggtgacacat ctctatgcgc cggcggagtt catcaagcag gtcagggtga
     3241 gcttcgaagg ag
//
LOCUS       DASUOM010000569         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_168653,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000569 DASUOM010000000
VERSION     DASUOM010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168653"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..561)
                     /gene="apaG"
                     /locus_tag="VFY27_11890"
     CDS             complement(181..561)
                     /gene="apaG"
                     /locus_tag="VFY27_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5766263.1"
                     /translation="MSDNDFEISIEVATDYVDDQSEPDVGRYVFAYTITISNRGNVPA
                     KLLSRHWLITDANGKVQEVRGDGVVGEQPHLNPGEKFRYSSGAVLETPVGAMQGSYRM
                     KADNGISFDAPIPPFTLAVPGVLH"
     gene            complement(611..1435)
                     /gene="rsmA"
                     /locus_tag="VFY27_11895"
     CDS             complement(611..1435)
                     /gene="rsmA"
                     /locus_tag="VFY27_11895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5766264.1"
                     /translation="MRPNAPRKRFGQHFLAAEETISQIVAAVSPREGDTIVEIGPGRG
                     AITGDLAKSGAAVHAVEFDRDLVPWLRERYAQLGNISIHEADALRFDFPSLGSELRIV
                     GNLPYNISTPLLFRMADISGSLRDLHFMLQKEVVDRITASPGTGAYGRLTIMLGCRMH
                     SEQLFDVPATAFRPPPKVTSSFIRLWPRKADEMPVTDRKLMSQLVAQAFSQRRKTLRN
                     ALRNTATPEDLAAVQIDPGSRPGEVPVTAWVTLANRLAARERAPNPRGTGGQMGIE"
     gene            complement(1432..>2307)
                     /gene="pdxA"
                     /locus_tag="VFY27_11900"
     CDS             complement(1432..>2307)
                     /gene="pdxA"
                     /locus_tag="VFY27_11900"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5766265.1"
                     /translation="ADPETLLARAAMLGWNDVTLREFDPAGKRAVHARTGELLVMSQP
                     LPNPDVCGTPDPQNARSLLEGIKRAVDGCTSGTFAALVTAPLQKSAINDGGIAFSGHT
                     EYLAELTAAATPVMLLVAADLRVALASTHMPLKDVSAYLTPERLARVIEVLDHDLQTK
                     FRIPKPSILVCGLNPHAGESGHLGREEREVIAPVVRKMQERGIDVRGPVPADTAFTPA
                     AGKSDAVLAMYHDQGLPVLKYAGFGTAVNVTLGLPIVRTSVDHGTALDIAGTGKADPG
                     SMLAAVELAASLSAA"
BASE COUNT          390 a          705 c          765 g          447 t
ORIGIN      
        1 cgcaaagccc ccatggatgg gttcacggcg ttcctgacaa gccctccccc caaccaaggc
       61 gtagccaacc acggacgtta cccgttcacg agctgccgtc ttcacccgtt catgagcaat
      121 tgacgtgtgg tgcccgttca cgagcgatgc aggcattcgg ctgttcgtca gcaacgctgg
      181 ttagtgcagt acaccgggta cggcgagggt gaaggggggt atgggggcat cgaagctgat
      241 gccgttgtcg gctttcatgc ggtagctgcc ctgcatcgcg ccgacgggcg tttcgaggac
      301 agcgccgctc gagtagcgga atttttcgcc cgggttcaaa tgcggctgtt cgccgacgac
      361 gccgtcgccg cgaacttcct gcaccttgcc gttcgcgtcg gtgatcagcc agtggcggct
      421 aagcagcttt gccggaacgt tgcccctgtt ggagatcgtg atcgtgtagg caaagacata
      481 gcggccgaca tcgggttccg actggtcgtc gacgtagtcg gtcgccactt cgatactgat
      541 ctcgaaatcg ttgtctgaca ttggcggctg caatgcaact cccatgatcg cacggcggat
      601 tggcttgggc tcattctatc cccatttggc cgccggtgcc ccggggattg ggggcccgct
      661 ctcgagcagc gagccggttc gccagcgtca cccatgccgt caccggcact tccccggggc
      721 gtgaccccgg gtcgatctgc acggctgcca ggtcttccgg cgtagccgtg ttcctgaggg
      781 cgttgcgcag ggtcttgcgc cgctggctga atgcctgcgc gacgagctgc gacatcagct
      841 tgcgatccgt gaccggcatc tcgtccgcct tgcggggcca gagcctgatg aacgaagagg
      901 tgacctttgg aggcggcctg aatgccgtgg caggaacatc gaacagctgt tcggaatgca
      961 tgcggcatcc cagcatgatc gtgagcctgc cgtacgcgcc ggtgccaggg gaggccgtga
     1021 tgcgatcgac gacttccttc tgcagcatga agtgcaaatc gcgcaacgaa cctgagatgt
     1081 ccgccatgcg gaagagcagg ggagtcgaga tgttgtacgg aaggttgccg acgatgcgca
     1141 gttcgctgcc gagtgacggg aagtcgaatc gcagcgcgtc tgcttcgtgg atgcttatgt
     1201 ttccgagctg tgcgtaacgc tcgcgtagcc acggtacgag atcgcggtcg aattcgaccg
     1261 catgcaccgc ggcgcccgat tttgcgaggt caccggtgat cgcgccgcgg ccgggaccaa
     1321 tctcgacaat ggtgtcgcct tcccgcggag acacggccgc gacgatctgc gatatcgtct
     1381 cttccgctgc gaggaaatgc tggccgaagc gtttgcgtgg tgcattcggc ctcatgcggc
     1441 agagagcgag gcggcgagtt cgaccgccgc aagcatgctg cccgggtctg ccttgccggt
     1501 gcccgcgatg tcgagggccg tgccgtgatc caccgaggtg cgcacgatag gcagaccgag
     1561 ggtcacgttc acggctgtcc cgaagccggc gtacttgagt accggaagcc cctggtcgtg
     1621 atacatggcg agcacggcgt ccgacttgcc ggcggcgggg gtaaacgcgg tatcagccgg
     1681 cacgggaccg cgcacgtcga tcccccgctc ctgcattttc ctgacgaccg gagcaattac
     1741 ctcccgttct tcacggccga gatggccgct ttcgccggca tggggattca ggccacacac
     1801 gaggatcgag ggtttcggga tcctgaactt cgtctgcagg tcgtgatcga gaacttcgat
     1861 tactcgcgcc agccgttctg gcgtcaggta ggcggagacg tcctttagtg gcatatgcgt
     1921 gctcgcgagc gccacgcgca ggtccgcggc cacgagcagc atgacgggag tagctgcagc
     1981 ggtcagttcc gcgagatatt cggtatgccc gctgaaggcg atgccgccat cgttgatcgc
     2041 gctcttctgc aagggggccg tcacgagggc agcgaaagtg ccgctggtgc agccatccac
     2101 cgcgcgtttt attccctcga gcagcgagcg cgcattctgc ggatcgggag tgccgcagac
     2161 atccggattc ggcagcggct gggacatgac cagcagctcc ccggtgcgtg cgtgaacagc
     2221 ccgcttcccg gccggatcga attcgcgcaa tgtgacgtcg ttccagccga gcatggccgc
     2281 gcgtgcaagg agcgtttcgg gatccgc
//
LOCUS       DASUOM010000570         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1765134,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000570 DASUOM010000000
VERSION     DASUOM010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765134"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFY27_11905"
     CDS             <1..211
                     /locus_tag="VFY27_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEX5766266.1"
                     /translation="IMSDQSHQIPAQLQTGRDVGMQLMDQALFAAIEANEVDPDDAFR
                     YASDKRKFKRFVTDTGILPTLDMDD"
     gene            236..1297
                     /locus_tag="VFY27_11910"
     CDS             236..1297
                     /locus_tag="VFY27_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5766267.1"
                     /translation="MNKIDQYLKDALVKSASDLHFISGDPPRVRIHGTLSKLNEQTLT
                     IEAVREALYEIMSGTMQRDFEKNDAIDFAYEIPDVSRFRVNIMRHLNGLGGVFRAIPS
                     KALTIEELKMPEVVHNLCKQTQGMILVTGKTGSGKSTTLAAMVDAINKRLKGHILTIE
                     DPIEFVHKRQGCLISQREVGVHSQSFAAALHSALREDPDVILVGEMRDLETISIAVTA
                     AEMGILVMGTLHTNGAAQTVDRIINSFPADKQGHIRTMLSTSLRGVISQQLLPTKHKP
                     GRVAALEILINTSAVANLIRQGRLDQLETAMQSGGAIGMQTMDSALMDLVERRFVSGK
                     EAYQQAHNKTKFQQIKDQT"
     gene            1546..>2457
                     /gene="metG"
                     /locus_tag="VFY27_11915"
     CDS             1546..>2457
                     /gene="metG"
                     /locus_tag="VFY27_11915"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5766268.1"
                     /translation="MPAPVRKILVSSALPYANGSIHLGHMVEYVYSDIWARFQKLRGH
                     ECTYVCASDAHGTPIMIKAREDGITPEELIGRVSQEQERDLEAFGVAFDNFHSTHSEE
                     NEELVGRMYLALRDAGHIYTRTIEQAYDEKEKMFLPDRFVRGTCPRCGSEDQYGDACE
                     VCGATYSPNDLVNPVSVLSGTTPLWRESEHYFFRLSEFGQRLKAWMKTAALHHSITSK
                     LEEWFEAGLKDWDISRDAPYFGFLIPGTGDKYFYVWLDAPMGYVASFLNLCRRQTNLD
                     FDKYWLPGHDTEVYHFIGKDIVYFHTLF"
BASE COUNT          525 a          774 c          719 g          439 t
ORIGIN      
        1 catcatgtcc gaccaatcgc accagatacc cgcacagtta caaaccggcc gcgacgtcgg
       61 catgcagctg atggaccagg cgttgtttgc ggccatcgaa gccaacgagg tcgatcccga
      121 cgatgcattc cgctacgcca gcgacaagcg gaagttcaag cgcttcgtga cggacactgg
      181 gatcctgccg acgcttgaca tggacgactg acggaccgga gccggacgac atcccttgaa
      241 caagatcgac caatacctga aagacgcgct ggtcaagagc gcctccgacc tgcacttcat
      301 ctccggcgat ccgccgcgcg ttcgcatcca cggcaccctg tcgaagctga acgagcagac
      361 gctgacgatc gaggcggtgc gtgaagcact ctacgagatc atgtccggca ccatgcaacg
      421 ggatttcgag aagaacgacg ccatcgactt cgcctacgaa atcccggacg tttcgcgctt
      481 tcgcgtcaac atcatgcgcc atttgaacgg cctcggcggc gtcttcaggg caataccgtc
      541 gaaagcactg acgatcgaag aactgaagat gcccgaggtc gtacacaacc tgtgcaagca
      601 gacgcagggc atgatcctgg tcaccggcaa gaccggctca ggcaagtcca cgacgctcgc
      661 tgcaatggtc gacgccatca acaagcgact caagggacac atcctcacca ttgaggatcc
      721 gatcgagttc gtgcacaagc ggcagggatg cctcatcagc cagcgcgaag tgggcgtcca
      781 cagccagtcc ttcgccgccg cccttcactc cgccttgcgc gaagacccgg atgtcatcct
      841 cgtcggtgaa atgcgcgacc tcgaaaccat ctccatcgcg gtaacggctg ctgaaatggg
      901 cattctcgtc atgggcaccc tgcacaccaa cggcgccgcc cagaccgtcg atcgcatcat
      961 caattccttt cctgccgaca agcagggcca tatccgtacg atgctgtcga cgtcgcttcg
     1021 cggcgtcatc tcgcaacagc tcctgcccac caagcacaag ccggggcgcg tcgccgcgct
     1081 ggaaatactc atcaacacct ccgctgtcgc caacctcatt cgccaggggc ggctcgacca
     1141 gctcgaaacg gctatgcaga gcggcggcgc gatcggcatg cagacgatgg actctgccct
     1201 catggacctc gtggagcggc gcttcgtctc gggcaaggag gcctaccagc aggcccacaa
     1261 caagacgaag ttccagcaga tcaaggacca gacttagcgg ccgcttcggt tcgggttttg
     1321 aaccctcgag gcattgttca tgaacggggt gaggtggtga ctggcggagg ctcgcccggc
     1381 ggggtatggc gttcctgaac agccctccca ccggacgagg cgctgccgac caccgccgcc
     1441 gaacgttcat gaatcgtgcg cacgcgcgac gcacatccga acagccacta ctcgccatgg
     1501 cataatcgcc agctttaacc ctcccgagcc ccagcagttc cgtctatgcc agcccccgta
     1561 agaaaaatcc tcgtcagcag cgccctcccc tacgccaacg ggtcgataca cctgggccac
     1621 atggtggaat acgtgtactc ggatatctgg gcgcgcttcc agaaattgcg cggccatgag
     1681 tgcacttacg tctgtgcgag tgacgcgcac ggtacgccga tcatgatcaa ggcgcgcgag
     1741 gacggcataa cacctgagga actgatcggg cgggtgtcgc aggagcagga gcgggacctg
     1801 gaagcgttcg gtgtcgcgtt cgacaacttt cattcgacgc actcggaaga aaacgaagag
     1861 ctggtcggcc ggatgtacct ggctttgcgc gacgcggggc atatctatac caggacgatc
     1921 gagcaggctt atgacgagaa ggagaagatg ttcctgccgg atcgtttcgt gcgcgggacc
     1981 tgccctcgct gcggcagcga agaccagtac ggcgatgcct gtgaggtctg cggtgcaacg
     2041 tactcgccga acgaccttgt gaatccggtt tcggtgttgt ccgggacgac gccgctgtgg
     2101 agggaatccg agcactattt tttccgactg agcgagttcg ggcagcggct gaaggcctgg
     2161 atgaagacgg cggcgcttca ccattccatc accagcaagc tggaggagtg gttcgaggcg
     2221 gggctcaagg actgggacat ctcgcgcgat gctccctact tcgggttcct gattcccggc
     2281 accggggaca agtatttcta cgtctggctc gacgcgccga tggggtatgt cgccagcttc
     2341 ctgaacctgt gccggcggca gaccaacctc gacttcgaca agtactggct gccgggacat
     2401 gatacggagg tttatcactt catcggaaag gacatcgtgt acttccacac gctgttc
//
LOCUS       DASUOM010000571         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_535158,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000571 DASUOM010000000
VERSION     DASUOM010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535158"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFY27_11920"
     CDS             complement(<1..871)
                     /locus_tag="VFY27_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766269.1"
                     /translation="MRQSFSSFIVLAFLFLALNGCGDTATLPVDAGIGPNPQLPEANP
                     SLIPTVNIAPAKGWEDGATPTPAAGLAVVAFARSLDHPRWLYVLPNGDVLVAESNAPV
                     RPAARKGIKGWMMKRIMERAGAGVPSADRITLLRDSDGDGIAETRSVFLENLSSPFGM
                     ELVGNDFYVANADALVRFTYREGSTTIEGPGVTVAALPGGPINHHWTKNVLASTDGRV
                     LYVTVGSNSNVAENGMENEVHRAAILEVDPGGGGLRLYASGLRNPNGLAWNPADGSLW
                     VTVNERDGIGSDLV"
     gene            987..1820
                     /locus_tag="VFY27_11925"
     CDS             987..1820
                     /locus_tag="VFY27_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX5766270.1"
                     /translation="MAISTASLFLILLVLALVLLWTVPPRLRRSRRARAFGRPFPVVF
                     AEILERNLPLYRKMPADLQSELRGRINVFLDEKVFIGCGGLEITDEIRVTIAGHACCL
                     ILNRDADFFPGFSSILVYPDAYLVDEVVSDGVLETEGQDARSGESWHRGPVVLSWQDV
                     LDSLAGDSDGYNVVLHEFAHKLDEENGEVNGVPALAETSHYKEWADVLTRAYSASNTA
                     GGKHGVRVLDEYAFTAPEEFFAVATETFFEKPAQMQKELPDLYRQLRRFYCVDPASWT
                     G"
     gene            complement(1872..>4003)
                     /locus_tag="VFY27_11930"
     CDS             complement(1872..>4003)
                     /locus_tag="VFY27_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766271.1"
                     /translation="YLKGKTDFDSLPPRSPPYWGHYVFHDNNRDTHQKTLEITRAVHR
                     MFYEYHPTVVHDLHESIPLLLTWNGTGPFNEYIDPILIDEWFEMAFAEIRTMTTFGMP
                     GVWTFAFGEAWGHHYLDSVAVNHNAIGRGYETFGNATAETVDRVLRVDEQKDDADNAV
                     TTQQWYRPLPPERNVRWSLRNNTNYMQTGALAVLEHSAGNAAEMLRAFYRKGYNSWQK
                     GINEAPYAFVIPADQGDPRRVAEMVGLLSGHRIEVGRAQSAFTIDEGRFPAGTFVVKL
                     DQPYRNYAVDLLKPQRFPSDTDYTPYDDVSWALPVHYGVEALEVADQGIRNVATTAVT
                     EDYLPTADVSGSGPVFLLKDSGQEAMLAARARLAAFDIDIAEQEFTVGRTRYPAGSWI
                     LPAQDGLREELDEVAAELALNFQGVRSLPDVARHAAPLPRIAVWHTWDDTESVGWIRY
                     SLDQREIPYDYIRDDDVRAGNLDQYDIILFGNNYLDLKDQIHGIDKRFGPMPYTRTEE
                     FPSHGVPDSSEDITGGIGWEGMANLERYLAGGGLMITLGNGSALALEGGLVAGVAATD
                     NEAVTPGSELTTTFLRTDHPIAYGYSEKASAFRSSFTVYNVRRAERDRVVMQWGTKPI
                     KDDREEEPDEADEEEEEEEEAEMLVSGGIEKADDLEGYPAIIDLPAGRGKVIAFNFNP
                     MHRDLNRSDHRFLWNAILNWQHLLEMR"
BASE COUNT          672 a         1280 c         1247 g          804 t
ORIGIN      
        1 gcaccaggtc gctgccgatg ccgtcgcgct cgtttacggt aacccacagg ctaccgtcgg
       61 cgggattcca cgccagcccg ttcgggtttc gcagccccga cgcgtacaga cgcaggccac
      121 caccccccgg atccacctcg agtatggctg cccggtgtac ctcgttctcc ataccgttct
      181 cggccacgtt gctgttcgag ccgacggtga catacagcac ccttccgtcc gtgctcgcga
      241 ggacgttctt cgtccagtgg tggttgatcg ggcctcccgg cagtgcggcg accgtaacgc
      301 cgggcccctc tatcgttgtc gagccctcgc ggtaggtgaa gcgcaccagc gcatcggcgt
      361 tcgcaacata gaagtcgttg ccgaccagtt ccatgccaaa cggcgaactc aggttctcga
      421 ggaataccga acgggtttcc gcgatgccgt cgccatcgct gtcacgcaac agggtgatgc
      481 gatctgcgct cggaacgccc gcacccgcgc gctccatgat tcttttcatc atccagccct
      541 tgatcccttt cctcgctgcc ggccggacgg gcgcattgct ttcggccacc agcacgtccc
      601 cgtttggcag aacgtagagc cagcgcgggt gatcgagcga tctggcgaac gcaaccacgg
      661 ccaggccggc tgcgggcgtc ggggttgccc cgtcttccca gcccttcgcc ggcgcaatgt
      721 tcacagtcgg tatcagggag ggatttgctt cgggcaattg agggtttggg ccgatacccg
      781 cgtccacggg cagggttgct gtatcgccgc atccgttcag cgccaggaac aggaaagcca
      841 ggacaatgaa tgacgaaaag gactggcgca tgacggtctc cgtgcagtgc agagctcccc
      901 gcgtgccttt cgcagtatag cgctcgcctg ctcactcccg aagcgtcgcc agattcgtat
      961 actgtctgcc gggatcgaaa aggaggttgg caatttctac cgcttcactg ttcctgatcc
     1021 tgctggtgct ggccctggtc ctcttgtgga cggtgccacc gcggctcagg cgctcgcgtc
     1081 gcgcaagggc tttcggcagg ccattccccg ttgtgttcgc ggaaatcctc gagcgcaacc
     1141 ttccgctgta ccggaagatg cctgccgacc tgcagtccga actgcgaggg cggatcaacg
     1201 ttttcctcga cgaaaaagta ttcatcggct gtggcgggct cgagataacg gacgagatca
     1261 gggtcaccat cgccgggcat gcctgctgcc tcatcctgaa tcgcgatgcc gatttcttcc
     1321 ccgggttctc gagtattctc gtttaccctg acgcatacct tgtcgacgaa gtcgtgtcgg
     1381 acggggtttt agaaacggag gggcaggatg cacgttccgg cgagtcatgg catcgcggac
     1441 cggttgtgct ttcgtggcag gacgtgctcg acagccttgc cggggacagt gacggctaca
     1501 acgtcgtcct gcacgagttc gcgcacaagc tcgacgagga gaacggcgag gtaaacggcg
     1561 tgccggcgct ggccgagaca tctcattaca aggagtgggc cgacgtgctc acgcgcgcat
     1621 acagcgcctc taataccgcc ggcggcaagc atggcgtgcg ggtgctggac gagtacgcct
     1681 ttacagcacc ggaggagttc ttcgccgtgg cgaccgagac cttcttcgag aagcccgcgc
     1741 agatgcagaa ggaactgccg gacctgtacc ggcaactgcg caggttctac tgcgtggatc
     1801 ccgcgagctg gacgggatag ttgttctgga ccgccgctgc aggagaccgc cttgcgggcg
     1861 acgctatcgg ctcaacgcat ctccagcagg tgttgccagt tcaggatcgc gttccacaag
     1921 aagcggtggt cggagcgatt caggtcgcgg tgcatcgggt tgaagttgaa ggcaattact
     1981 ttgccgcgtc ccgccggcag gtcaataatc gccgggtaac cttccaggtc gtccgctttc
     2041 tcgatgccgc cgctcaccaa catctccgcc tcttcttctt cttcctcctc ctcgtccgcc
     2101 tcgtccggtt cctcctcgcg atcgtccttg atcggcttcg tgccccactg catgaccacg
     2161 cgatcccttt cggcgcggcg aacgttgtag accgtaaagc tgctgcgaaa agccgaggcc
     2221 ttttccgaat agccgtacgc gatcgggtga tcggtcctca agaatgtcgt ggtcaattcc
     2281 gaacccggcg tcactgcctc gttatcggtc gccgctacgc cggcaacgag cccgccctcc
     2341 agcgccagtg cagagccgtt gccgagcgta atcatcaggc cgccgccggc caggtaccgc
     2401 tcgaggttgg ccatgccttc ccagccgatg cctcccgtga tgtcctccga cgagtcgggc
     2461 acgccgtggc tcgggaactc ttcggtccgc gtgtacggca tgggcccgaa ccgcttgtcg
     2521 atgccgtgaa tctggtcttt caggtccagg tagttgttgc cgaacaggat gatgtcgtac
     2581 tggtccaggt tgccggcacg gacgtcgtcg tcgcgaatgt agtcgtaagg gatttcccgc
     2641 tggtccaggc tgtagcgaat ccagccgacg gactcggtgt cgtcccacgt gtgccaaacc
     2701 gcgatccgcg gcagcggtgc ggcatgccgc gcgacgtccg gcaggctgcg cacgccctgg
     2761 aagttcagcg cgagctccgc tgcgacctca tccagctctt cgcgcaaccc gtcctgcgcc
     2821 ggcaggatcc acgaacctgc gggataccgg gtgcgcccga cggtgaactc ctgctccgcg
     2881 atgtcgatat cgaacgcggc caatctggcc cgggccgcca gcatcgcctc ctgcccgctg
     2941 tccttcagga ggaatacagg accggaaccg gacacgtccg cggtgggaag gtaatcctct
     3001 gtaaccgccg ttgtcgcaac gttccttata ccctggtccg cgacctccag cgcttcgacg
     3061 ccgtagtgca ccggcagcgc ccaggagacg tcgtcgtatg gcgtgtaatc cgtgtcggat
     3121 gggaaccgct gtggcttcag gaggtcgacg gcgtagttcc ggtacggctg gtccagtttc
     3181 acgacgaaag tgccggcggg gaacctgcct tcgtcgatcg tgaacgcgga ctgcgcccgt
     3241 cccacttcga tgcgatgacc cgacagcagg ccgaccatct cggcgacgcg gcgggggtca
     3301 ccctggtcgg ccggtatgac gaacgcgtac ggagcttcgt tgatgccctt ctgccacgag
     3361 ttgtagccct tgcggtagaa agcacggagc atctccgcgg cgttgccggc gctgtgctcc
     3421 aggaccgcga gcgcgccggt ctgcatgtaa ttcgtattgt tgcgcagcga ccagcggacg
     3481 tttcgttccg gaggcagcgg gcgataccac tgctgcgtcg tgacggcgtt gtcggcgtca
     3541 tccttctgtt cgtcgacgcg cagtacgcga tccaccgtct ccgcggtcgc gttgccgaag
     3601 gtctcgtaac cacggccgat ggcgttgtgg ttgaccgcga ccgaatcgag gtagtggtgt
     3661 ccccaggctt cgccgaaggc aaaggtccag acacccggca tgccgaacgt cgtcatcgtg
     3721 cgaatttcgg cgaaggccat ctcgaaccat tcgtcgatga ggatggggtc gatgtactcg
     3781 ttgaagggcc ccgtgccgtt ccacgtcagg agcagcggaa tcgactcgtg caggtcgtgc
     3841 accaccgtcg ggtgatactc gtagaacatc cggtgcacgg cgcgcgtaat ctcgagggtc
     3901 ttctggtgcg tatcgcggtt gttgtcatgg aagacgtaat ggccccagta cggcggtgag
     3961 cgcggcggca ggctgtcgaa atccgtcttg cctttcaggt agc
//
LOCUS       DASUOM010000572         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_319672,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000572 DASUOM010000000
VERSION     DASUOM010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319672"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFY27_11935"
     CDS             complement(<1..413)
                     /locus_tag="VFY27_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX5766272.1"
                     /translation="MTSSLVHRGPDDCGFFSHNNVAFGFRRLSIIDLEGGNQPILNES
                     GDIVLVCNGEIYNSPDIRRKLASAGHRFRTRTDVEVILHLYEDHGEDCVRYLQGMFAF
                     AIWDMRSKRLLLARDHMGQKPLYFYQDNERFLFAS"
     gene            complement(504..1499)
                     /locus_tag="VFY27_11940"
     CDS             complement(504..1499)
                     /locus_tag="VFY27_11940"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5766273.1"
                     /translation="MTRERKVLVTGAGGFIGKHLVGDQLARGHEVTATDVNLGELEAM
                     KLDGRLTCRQVDVRSSKDMRPCMQGADVVFHLAAAHLEVTEDDSYFEDVNARATEELL
                     RIAAEEGVSRFVHCSTAGIYGPLKKLPANEETPAAPDIPYERSKLAAEKVVLAESLRT
                     GLHTVIMRPAWVYGPLCPRTLKLVRAIARRRFFFVGKGENLRHPIYITDILQAFERAA
                     TLAVPPGEKLLAAGPEAVTVRQLVAAIAEELGMAYSPPTIPAWLMKPVCFGVEKIAAI
                     AGAEPPFSRRSLKFFSESSAFDISRARRVLGFEPVVGIRQGLRLTIAFYRQRGLL"
     gene            complement(1496..2371)
                     /locus_tag="VFY27_11945"
     CDS             complement(1496..2371)
                     /locus_tag="VFY27_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5766274.1"
                     /translation="MMSVRDMEKGAEEPWQLAMFRKGLKKRLRLASLRKLLLPLPKDE
                     RCLLLTCGDNNGAMNYYLRELGGRWSWADLEKTSIAEMTRLLGEPVAHAAEDSLPYAD
                     ASFNRVVVIDVHEHVSEPAIVTREIARILKPGAQLIITTPNGDETKLAVRIKSLVGMS
                     VETYGHTRVGLTGDEIAELMTAAGVRPVGTTTFSGFFTEMLELTINFLYVKVLRRGPE
                     AAEVRDSPDGQHADGHADHSEIAPAPAEQLQKVQTSYRIYSAIFPLYWLISKLDHLLF
                     FRDGYCVVVEGKRER"
     gene            2565..2960
                     /locus_tag="VFY27_11950"
     CDS             2565..2960
                     /locus_tag="VFY27_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX5766275.1"
                     /translation="MTTELQCLAWVTILTLVIRIPWMVNKVTVRGLEKVTEYPLTSEP
                     LSGWAHRVWVAHEDALQNLIVFAVLVGLLHAAGESNGWTAGAAIIYFWARLVHFIVYA
                     FGIPRVKTIAFLAAFAAQLVLGWQVVMCL"
BASE COUNT          585 a          910 c          904 g          658 t
ORIGIN      
        1 tcggaagcaa aaaggaagcg ttcattgtcc tggtaaaagt acagcggctt ctgccccatg
       61 tggtccctgg cgagcagcag ccgcttgctg cgcatgtccc atatcgcaaa cgcgaacatg
      121 ccctgaaggt accgtacaca atcttcgccg tgatcctcat agaggtgaag gatcacttcg
      181 acatccgtac gagtacgaaa acgatgcccg gcggacgcca gcttccggcg tatgtcaggg
      241 ctgttgtaga tttcgccgtt gcagacgagc acgatgtcac cgctctcgtt caggatgggc
      301 tgattgcccc cctcgaggtc aataatgctc agcctgcgaa aaccgaacgc gacgttattg
      361 tgcgagaaga agccgcagtc atccgggccc cggtgaacaa gagaggaagt cattcgtcgc
      421 gtgagttctt cggaactctc gccgattgcg cctgcaattc cgcacatatc gtagcgtcct
      481 ttcggcgctt ttatccctgc tccttaaagg agcccgcgct ggcgataaaa cgcgatggtc
      541 aggcgtagcc cctgccggat tccgaccacc ggttcaaaac ccagtacgcg cctcgccctg
      601 gagatgtcga aagcggagct ttcactgaag aatttcaggc ttcgacgcga aaacggcggt
      661 tccgctcctg cgatcgcggc gattttttca acgccgaagc aaacgggttt catgagccat
      721 gccggtatcg tgggcggaga gtaggccatt cccagttcct ccgctatcgc cgccacgagt
      781 tgtcgcaccg taacggcttc cgggccggcg gcaagcagtt tttctccagg cggaacagcc
      841 agggtcgcgg ctcgctcgaa cgcctgcagg atatcggtaa tgtaaatcgg gtgccgcagg
      901 ttctcacctt tcccgacgaa aaaaaagcgc cgcctggcga tggcgcgaac cagcttcagg
      961 gtgcgcgggc agagcggccc gtacacccac gccggccgca tgatgacggt gtgcagtcca
     1021 gtccgcagcg actcagccag caccaccttc tcggccgcca gcttgcttct ttcgtacgga
     1081 atgtccggcg cagcgggcgt ctcttcgttc gccggcagtt tcttaagcgg cccatagatg
     1141 cccgcggtgc tgcagtggac gaagcgggac acgccctctt cagctgcgat cctcaggagt
     1201 tcttcagtgg cgcgagcgtt gacgtcctcg aaataggagt catcctcggt tacttcgagg
     1261 tgtgcggcgg caagatggaa tacgacatcg gctccctgca tgcagggccg catatctttg
     1321 ctcgagcgca catcgacctg ccggcaggtg agacggccgt cgagcttcat tgcctcgagc
     1381 tctccgaggt ttacgtcggt cgcggtcact tcatgacctc tggccaactg gtccccgacc
     1441 agatgtttgc cgatgaagcc tcccgcgccg gtaacgagta ccttacgctc tcgagtcatc
     1501 gctcgcgctt tccctcgact acgacacaat agccgtcacg gaagaaaagc aagtggtcga
     1561 gcttagagat aagccagtag agaggaaaga ttgcggagta aattcgatag ctggtctgca
     1621 ccttctgcag ttgttccgcg ggagccggtg cgatttcgga atggtctgca tgaccgtccg
     1681 catgctgacc gtcagggctg tcccgcacct cggcggcctc tggtccgcgg cgcaatacct
     1741 tcacatacag gaaattgatc gtcagttcga gcatctcggt gaagaagccc gagaatgtag
     1801 ttgtccctac aggccgcaca cccgcggccg tcatcagctc cgcgatctcg tcaccggtaa
     1861 gcccgacgcg ggtatggcca taggtttcca cggacatccc caccagcgac tttatcctga
     1921 ctgcgagctt ggtctcgtcc ccgttgggcg tcgtgatgat caactgcgca cccggcttca
     1981 atatccttgc tatctcccgt gtgacgattg cgggttcgga gacgtgttcg tgcacgtcga
     2041 ttacgacgac tcggttgaag cttgcgtcgg cgtacgggag cgagtcttca gcggcgtgcg
     2101 ccacgggctc gccaagcaac cgggtcatct ccgctatgga agttttctcg aggtccgccc
     2161 aggaccagcg tccgcccaac tcccgcaggt agtagttcat ggcgccgttg ttgtcgccgc
     2221 aagtgagcag gagacagcgc tcgtccttcg gtagcgggag aaggagcttc cttaaggatg
     2281 cgaggcggag cctcttcttg agtcccttgc gaaacatcgc gagttgccag ggctcttcgg
     2341 ctcccttttc catatcgcgg actgacatca ttaaaagtcg cagtgccttc ggatgcgttc
     2401 gttggctcaa tgtcgtggta acagacaagc ggtctgggcg tgcttcgctt gagatttatt
     2461 tccccggctg cggtgtctac ggagacttgg cacctgtttt cggctatcgt cgcgctgctc
     2521 ggagcaaaag acaaggaaat gcgtctcagc acgggacctt gcgaatgacc acagaactac
     2581 aatgcctcgc ctgggtaaca attctgacgc tggtgatccg gatcccatgg atggtgaaca
     2641 aggtgaccgt ccgcggtctc gaaaaggtta ccgagtaccc gctgacctcg gagcccctgt
     2701 caggctgggc ccaccgcgtc tgggtggcac acgaggatgc gttgcagaac cttatcgtct
     2761 tcgcggtgct ggtgggcctg ctgcacgcag ccggcgaatc gaatggctgg acggccggag
     2821 cggcgattat ctacttctgg gcgaggctgg tgcatttcat cgtgtacgcc ttcggcatac
     2881 ccagggtcaa gacgatcgcc ttcctggcag cctttgccgc gcaactcgtt cttggctggc
     2941 aggtggtcat gtgtttgtag agaggctgcc ttgctgaagc ggatgaacca aaggtatcag
     3001 ggtcaagctt ccgttttatc tcccgtctcg cgcacgcggg ggcctgctgc gtcgcgg
//
LOCUS       DASUOM010000573         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_705141,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000573 DASUOM010000000
VERSION     DASUOM010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705141"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFY27_11955"
     CDS             complement(<1..239)
                     /locus_tag="VFY27_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626550.1"
                     /note="DNA-(apurinic or apyrimidinic site) lyase; has
                     apurinic or apyrimidinic endonuclease activity and DNA
                     N-glycosylase activity; removed damaged DNA at cytosines,
                     thymines and guanines; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5766276.1"
                     /translation="MNPAKRIAIYSRLREQNPHPETELEYGSPFQLLVAVILSAQATD
                     VGVNKATARLFRDAPTAQDMLALDVAGLKRYIKTIG"
     gene            complement(261..932)
                     /locus_tag="VFY27_11960"
     CDS             complement(261..932)
                     /locus_tag="VFY27_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HEX5766277.1"
                     /translation="MNSGPATLPQVKAGLWSENPGLVQLLGLCPLLAVTTTFVNGLGL
                     GLATLFVLTCSNALVSATRRFIYSEIRIPMYVLIIASLVTCIEMIFEAWFPALDRSLG
                     IFIALIVTNCAIVARAELFASRNRLLPSIIDGLSMGAGFALLLCALGAFRELVGQGSI
                     LARMDMLLGGEPLRGLELSDRGFLLAILPPGAFFSLALAVAAKNALDRRRKQKLGLLA
                     ARQLS"
     gene            complement(929..1612)
                     /gene="rsxG"
                     /locus_tag="VFY27_11965"
     CDS             complement(929..1612)
                     /gene="rsxG"
                     /locus_tag="VFY27_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HEX5766278.1"
                     /translation="MMPGRSPSALSSGIVLAVVAAVCTALVAVTHRVTAPRIAENEKA
                     WLEQSLRPALADIFYDNELSESTLVLPPPHGLPGNEPATIYRLFSEERPVAAVFIVSA
                     RDGYSGPIRLLVAVEYSGRLTGVQVLEHNETPGIGDLIEPSRSDWLKQFEGASLEEPP
                     RGRWAIRRDGGAFDQFTGASITPRAVVRAVKETLLYYEANRDSVFEAPAQAETEQQDM
                     PVTDPETEQ"
     gene            complement(1609..>2427)
                     /locus_tag="VFY27_11970"
     CDS             complement(1609..>2427)
                     /locus_tag="VFY27_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HEX5766279.1"
                     /translation="AMVTAALIAFSLPALCPWWVTATAALFAIVVAKHLYGGMGYNIF
                     NPAMAGYVVALVAFPTYMNFWTAPRIGDIDYHYLNLYETARYTLTGSLPEYLDFDAIS
                     RATPLDTIKTGLQNMMTFDEMYSNPLMGDFGGRGWEWIGNFTAIGGAWLLLRKIIRWH
                     IPVAVLAGVLVPSGIMYFLDTGVHASPGFYLFSGGTILCAFFIATDPVTAATSPKGRL
                     VYGFGIGLLIYAIRRWGSYADGVAFAVLLMNMARPAIDYLTRPRIVGHARNEAR"
BASE COUNT          483 a          728 c          785 g          431 t
ORIGIN      
        1 ccaatagtct ttatgtagcg cttgagaccg gcaacgtcca gggcaagcat gtcctgcgcc
       61 gtcggtgcgt cccggaagag cctggctgtc gccttgttta cgccgacgtc cgtcgcctgc
      121 gccgacagta tcaccgccac cagcaactgg aacgggctac cgtattcgag ttccgtctcc
      181 gggtgcggat tctgctcccg caaacgggag tagatcgcaa tgcgtttggc ggggttcaag
      241 ggcggaggag gccggaacgc tcagctcagc tggcgtgcag cgagcagccc gagtttctgc
      301 tttcttcgcc ggtcgagtgc atttttcgcg gcaaccgcga gggcgaggct gaaaaacgcg
      361 cccggcggca aaatcgcgag caggaacccg cggtccgaga gctcgagccc gcgtaaaggt
      421 tcacccccga gaagcatgtc catgcgcgcg aggatcgagc cctggccgac cagttcgcga
      481 aacgcaccga gcgcgcacag cagaagcgca aagcctgctc ccatggaaag gccgtcgatg
      541 atgctgggta acagccggtt gcgtgaggcg aaaagctcgg cgcgcgccac aatggcgcaa
      601 ttcgtcacga tcagcgcgat aaaaattccc agcgatcggt cgagcgcggg gaaccacgct
      661 tcgaagatca tttcgatgca ggtaacgagg ctcgcgatga tcagcacgta catagggatg
      721 cggatctcgc tgtagataaa gcgccgggtt gcagacacca gcgcattgga gcacgtcagg
      781 acgaacagcg tcgcgaggcc gagtcccagc ccgttgacga aagtggtggt cacggccagg
      841 agcggacaca ggcccaacag ctgtactagg ccgggatttt cgctccacag cccggccttg
      901 acctggggca atgtcgccgg cccgctgttc attgctctgt ttccggatca gttaccggca
      961 tgtcctgctg ctccgtttcc gcctgggctg gtgcttcgaa cacgctgtcg cgattcgctt
     1021 cgtagtatag caacgtctcc ttgaccgccc tcacgaccgc ccgcggcgtg atcgaagctc
     1081 cggtgaactg gtcaaacgca ccgccgtcgc gccggatcgc ccagcggcca cgcggaggct
     1141 cctcgaggga tgctccctcg aactgcttca gccagtcaga acgcgatggc tcgatcaaat
     1201 cgccgatacc cggggtttcg ttgtgctcca atacctggac gccggtcagc cgacctgagt
     1261 actcgacggc gacgaggagt cgtatcggcc cggagtagcc gtcgcgggca ctgacaatga
     1321 acacggccgc caccggccgt tcttcagaga acaacctgta gatcgtcgca ggctcgttcc
     1381 cgggtagtcc gtgcggcggc ggaaggacga gcgtggattc ggacagttcg ttgtcgtaga
     1441 aaatgtcggc aagggccggt cgcaggcttt gctcgagcca ggctttttcg ttctcggcga
     1501 ttcgtggtgc ggtaaccctg tgcgtgacgg cgaccagcgc agtgcaaact gcagccacga
     1561 cagcgagcac gataccgctg ctcaatgcag agggcgagcg cccgggcatc atctcgcctc
     1621 gttcctggca tgaccgacga ttcgcggtct cgtcaggtag tcgatcgcag ggcgcgccat
     1681 gttcatcagc agtacggcga atgctacgcc gtctgcgtag ctgccccagc gccggatggc
     1741 gtatatgagc aagccaatgc cgaatccgta aacgagccgt cctttcgggc tggtggccgc
     1801 cgtgaccgga tcggtcgcaa tgaaaaacgc gcacaggatg gtgccgccgc tgaacaggta
     1861 gaagccgggg cttgcatgca cgccggtgtc gaggaaatac ataatgccgg aggggaccag
     1921 tactcccgca agcaccgcga cgggaatgtg ccagcgaatg atcttgcgca acagcagcca
     1981 ggcaccgccg attgccgtga agttgccgat ccattcccag ccacgcccgc cgaagtcgcc
     2041 catcaacgga ttgctgtaca tctcgtcgaa cgtcatcatg ttctgcaggc ccgtcttgat
     2101 ggtgtcaagc ggcgtggcgc ggctgatggc atcgaagtcc aggtactccg gcagcgatcc
     2161 ggtgagcgtg tagcgtgcgg tttcgtacag gttgagatag tgatagtcga tgtcgccgat
     2221 gcgtggcgcg gtccagaagt tcatgtaggt tgggaaggcg accagcgcga cgacgtaacc
     2281 cgccatggcg ggattgaaga tgttatagcc catgccgccg tacaggtgct tcgcaaccac
     2341 gatggcgaag agcgcggcgg tggcggtaac ccaccagggg cagagggcgg gtagcgagaa
     2401 ggcaatgaga gcggccgtga ccatggc
//
LOCUS       DASUOM010000574         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_860926,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000574 DASUOM010000000
VERSION     DASUOM010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860926"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2171)
                     /locus_tag="VFY27_11975"
     CDS             complement(<1..2171)
                     /locus_tag="VFY27_11975"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766280.1"
                     /translation="MLANPRMSSFITPAGGGHMKWRGYAMTRWQPQTDGINGGSLLYV
                     RDLDRMLSWSPAVPAAGPHLQVICGPDSVEFRDTRHDLLIRHQIAVAPFSDLELRKVS
                     ITNDADVPRRLMVCAYSEPVLTDAVADARHPAFSKLFIEDEYLSDQDALLFRRRPRAD
                     GEPSLCLACKAVAQPGFDVEIQFETDRREFIGRSGSRESPAALQSGRLRCRPGESLDP
                     AAVVGLVVTIPPLSTFQCAFVTGVGDDEIEVLQNLDRMSSIQAVNWAMDDARRNMQQE
                     LMLLEMTSQDVRRGSELFGNLVSPPRPDLARVFLMSPAMRAQPSLWRHGISGDRPLIT
                     VVISDTVDFNNLKNLIRALAACQALGVTADVVFLDESDSAYSHPTHDRLQKVIRHYLR
                     RTGAERQFGTYIVPAFSLDEAGRQAFATSSHIMLDLRMDDWYQQLAFQQRQQPVVPAF
                     LPQPGSPVRRDPVLPVERPGNLLLDNGLSGVSRASSDYVIYLEKGDTTPAPWCNILAN
                     PDFGTLISESGSACTWYRNSSEFSLTTWSNDAVLDRPGEALYMRDEETGRFWSPLAGP
                     ARDEEPYVARHGIGRSYFEHSSEGLYQSTEVYVDPEDPVKFVRLHLRNLWPRARRLTI
                     TYAAEWRLGNSGIAAGLHLVPQWDGDRSTLFVRNAFSASYPETQAFLTSNLPAHGITF
                     DGDEFLGRSRSWAAPAGLRAIGLSGRVGPCAQPLAAYQVHV"
BASE COUNT          427 a          667 c          714 g          411 t
ORIGIN      
        1 ctgacatgaa cctggtaggc ggccagcggt tgtgcgcaag ggccaacgcg gccggacaag
       61 ccgatcgccc gcaacccggc cggcgcagcc catgaacggg aacgtcccag gaattcgtcg
      121 ccgtcgaacg taatgccgtg cgcgggaagg ttgctcgtga gaaacgcctg agtctccggg
      181 tagctggcgg aaaacgcatt cctgacgaag agggtgctgc gatcgccgtc ccactgcggt
      241 accaggtgca gcccggctgc aataccgctg ttcccgagac gccactccgc agcgtatgta
      301 atagtaaggc gacgcgcacg cggccacaga ttccggaggt gcaggcgcac gaatttcact
      361 ggatcctcgg ggtctacgta cacctctgtc gactggtaga gcccctcgct cgagtgttcg
      421 aaataactgc gcccgatgcc gtggcgcgcc acgtaaggtt cttcgtcgcg agcgggccct
      481 gcgagtggcg accagaagcg cccggtttcc tcgtcgcgca tgtagagcgc ttcgcccggg
      541 cggtcgagga cggcgtcgtt gctccacgtt gtaagtgaga actcgctgct gttgcggtac
      601 caggtgcacg cactgccgga ctcgctgatc agggtgccga agtccgggtt cgcgagaatg
      661 ttgcaccatg gggcaggtgt cgtgtctcct ttctccaggt agatgacgta gtcggacgag
      721 gcgcgtgaga cgccgctcaa cccgttgtcc agcaaaaggt tcccgggccg ttcgacgggg
      781 aggaccggat cccgccgcac aggtgaaccc ggctggggca ggaacgccgg gacgaccggc
      841 tgctgccttt gctggaacgc aagctgctgg taccagtcat ccatgcgcag gtccagcatg
      901 atgtgggagc ttgttgcgaa ggcctgtcgc ccggcttcat cgaggctgaa tgccggcacg
      961 atatacgtgc cgaactggcg ctcggcgccg gttctgcgca aataatgccg gatgactttt
     1021 tgcagtcggt cgtgagtcgg atgcgaatac gcgctatcgc tctcgtccag gaagactaca
     1081 tcggcggtga ctcccaaggc ctggcatgcg gcaagcgcac gaatcaggtt cttcaggtta
     1141 ttgaagtcga ccgtatcact tatgaccacg gttatgaggg gtcgatcgcc agatatccca
     1201 tggcgccaca gcgacggctg tgcgcgcatt gctggtgaca tcaggaacac tcgtgccaga
     1261 tcgggacgcg gcggcgatac gagattgccg aagagctcag aaccgcgacg gacatcctgc
     1321 gatgtcatct ccagcagcat cagttcctgc tgcatatttc gccgggcatc gtccatggcc
     1381 cagttgacgg cctggataga gctcatgcgg tccaggttct ggagtacctc gatttcatca
     1441 tcgcccaccc ctgtaacgaa tgcgcactgg aaggtcgaga gcggcggtat ggtgaccact
     1501 aagccgacga cagcggccgg atcaaggctc tcgccggggc ggcaacgtag ccggccggat
     1561 tgcagggccg cgggggactc gcggctcccg ctccggccaa tgaactcccg gcgatccgtc
     1621 tcgaactgga tttcgacgtc aaaccccggt tgagctaccg ctttacaggc gaggcaaagc
     1681 gagggttcgc cgtctgctct cggtcgacga cggaacagga gtgcatcctg gtcggaaagg
     1741 tactcgtctt cgatgaataa cttgctgaaa gccgggtgac gcgcatccgc aactgcgtcc
     1801 gtcagtaccg gctcgctgta cgcgcaaacc ataagtcgcc gcggcacgtc cgcgtcattc
     1861 gtgatgctga ccttgcgcag ttccaggtcg gaaaacggcg ccactgcgat ctgatgccgg
     1921 atcagcagat catggcgggt gtcccggaac tcgaccgaat cgggcccgca aatcacctgg
     1981 agatggggtc cggcagccgg gacggccggc gaccacgaaa gcatgcgatc gaggtcccga
     2041 acgtacagga gcgagccgcc gttgatcccg tccgtttgcg gttgccagcg tgtcatagcg
     2101 tatccgcgcc acttcatgtg gccgccgcct gcaggagtta tgaaagaaga catccgcgga
     2161 ttcgccagca tcgttatgtt tttcgaggag gcgtcgagcg accagtattc gatggccac
//
LOCUS       DASUOM010000575         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_922063,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000575 DASUOM010000000
VERSION     DASUOM010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922063"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1083)
                     /locus_tag="VFY27_11980"
     CDS             complement(58..1083)
                     /locus_tag="VFY27_11980"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX5766281.1"
                     /translation="MTPSPRTTRVIEGVPPDAIPYADLLAAERPAVLKGVVRDWPLAS
                     AASPVEAASYLKSFYQGRPVLAFIARPELKGRFGYTEDANKLDFTSERGSLVDYLDII
                     LGLAQDRDAPSYYIGSTDVDAYLPGFRAENGLILNHSMFASNPPMVGAWIGNRTTALA
                     HHDMSHNIACCAVGRRRFTLFPPEQVHNLYPGPLEPTPGGQVVTMTDIQAPDFDRFPR
                     LREALAVSEVADLEPGDALFYPAMWWHQVDALDAFNVMINYWWNTAPAYIDTPQNALL
                     HALLSVRDRPEQEKRAWRAMFDYYVFGPPERAGEHLPSDARGNLGPIDEIKARRLRAY
                     LLKRLNR"
     gene            complement(1093..1818)
                     /locus_tag="VFY27_11985"
     CDS             complement(1093..1818)
                     /locus_tag="VFY27_11985"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX5766282.1"
                     /translation="MTNSVLLDNVTHKDLRVKTGYSAEFGDSINSCVVFPTEFAYVQR
                     EYPIVFRHDAQGDLQAIALLGFDENENLFLDEKGWNARYVPAIQQRGPFLIGLQKGDS
                     GEERREAMVNVNLDHPRISQTEGEPVFLPHGGNSPYLEHMSRVLQLIYKGAGITRLMI
                     AAFEEAGLIESVDVNVEIQRGPTFRLPGLLTVSQEKLAGLDGAQLVRLNSAGFLQLAV
                     LAMASVGNISWLIELKNRKRAAA"
     gene            complement(2000..>3559)
                     /locus_tag="VFY27_11990"
     CDS             complement(2000..>3559)
                     /locus_tag="VFY27_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766283.1"
                     /translation="RATEIYVRSRSTQQDLGSWGMANPRDIEQYAPGVVEEFCMACEF
                     DEFPGGDTDIVFGGDATQLFTIMTPIYEQLGNPVTVNDAENWLEEDIMSVYAQFSTDT
                     EFMGRDLQVNGGLRLERTEVSATALQPVPTNVRWTADNDYVVDYGAGQENVEGEGDYN
                     HLLPNIDFRLDVTDQVVARASYSQTIGRVPFGNLFASTTVEPPNRPTIIGGVTGGTAQ
                     DPTLLPLESENFDLSVEWYYGDASYVSLGFFDKTVQNFLGTAQVDRPLFGLRDPTAGG
                     PGTRIEDALGIINGLGVDESEANLFTLVALMTANGGDLAAAQAEFEANLDANGVLPQL
                     YVDEIAAAYDVSGDATDPEMIFRVQQPINQEEGNVNGWEFSLQHFFGDTGFGFAANYT
                     IVEGDVEADDAIDPNANQFALVGLSDTANLTLIYENYGFAARVAYNWRDNFLNATNQG
                     GGNSPQYTGEYGQIDASVSYDLSDNIQLMFEGINLNGEDTRQYRRKESMFIWGYENEP
                     RYAFGARYRFN"
BASE COUNT          649 a         1112 c         1098 g          700 t
ORIGIN      
        1 ccgtgccgcc tccggcaatg acgacgcgct taacgacctg gctcgacaag ggcaccatca
       61 ccggttcagg cgcttcagca ggtaagcccg caggcgccgg gccttgatct cgtcgatcgg
      121 ccccaggttg ccgcgcgcgt cagacggcag gtgctcgccc gctcgttccg gcggaccgaa
      181 aacgtaatag tcgaacatcg cgcgccacgc tcgcttctct tgttccggcc gatcccggac
      241 gctcagaagc gcgtgcaaca gcgcgttctg cggcgtgtcg atgtaggccg gcgccgtatt
      301 ccaccagtaa ttgatcatca cgttgaacgc gtccagcgca tccacctggt gccaccacat
      361 cgccgggtag aacagcgcgt cgccgggttc gagatccgca acttcactca cggcaagcgc
      421 ctcgcgcagc cgcggaaacc gatcgaaatc cggcgcttgt atgtcggtca tcgtgaccac
      481 ctgcccaccc ggcgtcggct ccagcgggcc gggatacagg ttatgcacct gctccggagg
      541 gaacaaggta aaacgccggc ggcccaccgc gcagcatgcg atattgtgcg acatgtcgtg
      601 atgcgcgagc gccgtcgttc ggttaccgat ccaggcgcca accatcggcg gattgcttgc
      661 gaacatcgag tgattgagta tcaggccgtt ctcggccctg aatcccggca ggtaggcatc
      721 gacatcggtg gagccgatgt agtacgacgg tgcatccctg tcttgcgcca ggccaaggat
      781 gatgtcgaga taatcgacca gggagccgcg ctctgaagtg aaatcgagct tgttcgcgtc
      841 ttcggtatag ccgaagcggc ctttcagttc cggacgtgcg atgaacgcga gcaccggccg
      901 tccctggtag aacgacttca ggtagctggc cgcctcgacc ggcgaggcgg cgctcgcaag
      961 cggccagtcg cgcaccactc ccttgagtac ggcgggtcgt tcggcggcca ggagatcggc
     1021 gtagggaatg gcgtccggcg gaacgccttc gatgacccgt gtcgtgcgag gtgacggtgt
     1081 catgtgaggg agctaagcgg ccgcacgctt ccggttcttg agctcgatca gccaggagat
     1141 attgccgacg gaggccatgg ccaatacggc aagttgcagg aagcccgcac tgttgagccg
     1201 caccaactgg gcgccgtcga gtccggcaag tttttcctgg ctgacggtca gcaggccggg
     1261 cagcctgaac gtcggcccgc gttggatttc tacgttcacg tcgaccgact cgatgaggcc
     1321 cgcttcctcg aacgcagcaa tcatcagccg ggtgatcccg gcgcctttgt agatgagctg
     1381 aaggacgcgg ctcatgtgct cgagatacgg cgaattccct ccgtgcggaa gaaagaccgg
     1441 ttccccctcg gtttggctga tgcgtgggtg atccaggttg acattgacca tcgcttcccg
     1501 gcgctcctcg ccgctgtcgc ccttctgcaa gccgatcagg aacggtccgc gttgctggat
     1561 tgccggaacg tagcgggcat tccacccttt ttcgtcgagg aacaggtttt cgttctcgtc
     1621 gaagccgagc aaggcaatgg cctgcaggtc gccttgcgca tcatggcgga aaacgatcgg
     1681 gtactcgcgc tgaacgtagg cgaattccgt agggaaaacc acgcagctgt tgatgctgtc
     1741 gccgaactcc gccgaatagc cggtcttcac cctgaggtcc ttgtgggtga cattgtcgag
     1801 caaaaccgaa ttcgtcatct cgggtttccg gcggatggcg tcaggtccct tgtcaggcgg
     1861 ggtcgggctc ccgcaagtgt agcaaagcta ccgggacacg cgggcggaca gccgccacga
     1921 agcaggggat cgcatttcct cccgcagaaa aaatgaaggg tcgccgtgcg gcgacccttc
     1981 cgtcacgcac gaaccgtacc tagttaaacc gatagcgcgc accgaaagca tagcgcggtt
     2041 cgttttcgta gccccagatg aacatcgact ccttgcgacg gtattggcgg gtgtcctcgc
     2101 cgttcaggtt gatgccctcg aacatcagct gaatgttgtc ggacagatcg tatgagacgc
     2161 ttgcgtcgat ctggccgtat tcgcccgtgt actgcgggct gttgccgccg ccctggttcg
     2221 tcgcgttcag gaagttatcg cgccagttgt aggccacccg tgccgcgaaa ccgtagttct
     2281 cgtagatcaa cgtcaggttc gccgtatccg aaaggccgac cagggcaaac tggttggcat
     2341 tggggtcgat cgcgtcgtcc gcttccacgt cgccctcgac gatcgtgtag ttcgccgcaa
     2401 agccgaagcc cgtatcgccg aagaagtgct gcaaggagaa ttcccacccg ttgacgttgc
     2461 cttcctcctg gttgatcggc tgctgtaccc ggaagatcat ttccgggtcg gttgcatcgc
     2521 cggagacgtc gtaagcggcc gcaatctcgt ccacatagag ctgcggcagt acgccgttcg
     2581 catccaggtt cgcctcgaac tctgcctgtg ccgcggccag gtcaccaccg tttgccgtca
     2641 tcaatgcgac cagcgtgaac agattggctt cagattcgtc gacgcccagg ccgttaatga
     2701 tgcccagcgc atcctcgatg cgtgtacccg gcccgcctgc cgtcggatcg cgcaggccga
     2761 acaacggccg gtcgacctgc gccgtaccga ggaagttctg caccgtcttg tcgaagaatc
     2821 cgagagacac gtagctcgca tcgccgtagt accactcgac cgacaggtcg aagttctcgg
     2881 actcgagcgg caacaaggtc gggtcctgcg cggtgccgcc cgttacacca ccgatgatcg
     2941 tcgggcgatt cggcggctcc accgttgtcg acgcgaacag gttgccgaag ggcacgcggc
     3001 cgatcgtctg gctgtaggat gcgcgagcga cgacctggtc ggtgacatcg aggcggaagt
     3061 cgatattcgg cagcaggtga ttgtagtccc cttcgccctc gacattctcc tgcccggcgc
     3121 cgtagtcgac cacgtagtcg ttgtccgccg tccaccgaac gttcgtcggc accggctgca
     3181 gtgccgtcgc cgagacctcc gtacgttcca ggcgcaggcc gccattaacc tgcaggtccc
     3241 ggcccatgaa ctcggtgtcg gtcgagaact gcgcgtagac ggacatgatg tcctcttcga
     3301 gccagttttc ggcatcgttg acggttacgg gattgccaag ttgttcgtaa attggcgtca
     3361 tgatcgtgaa caactgcgtg gcatcgccgc cgaatacgat gtcagtatcg ccgcccggaa
     3421 attcgtcgaa ttcgcaggcc atgcagaatt cctcgaccac gccgggcgcg tattgctcaa
     3481 tgtcccgcgg gttggccata ccccagctgc cgaggtcctg ctgcgtcgag cggctgcgca
     3541 cgtagatttc ggtagcgcg
//
LOCUS       DASUOM010000576         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1693950,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000576 DASUOM010000000
VERSION     DASUOM010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693950"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFY27_11995"
     CDS             <1..374
                     /locus_tag="VFY27_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5766284.1"
                     /translation="LESTYMGAVLLDGMEAICSVWPRGVLDADLRDPLATDKPVLLLS
                     GEADPVTPPQFAEMALLEMTNSLHLVGADQGHGQAGRTCIPEIMAQFVETASVEGLDE
                     ECLAERQFAMPFFLDYNGPSP"
     gene            371..1105
                     /locus_tag="VFY27_12000"
     CDS             371..1105
                     /locus_tag="VFY27_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766285.1"
                     /translation="MIRVSNLKKSFGEVKAIDGVSFEAPDGLITGLLGPNGAGKSTTL
                     RVLYTVLKPDEGNADIDGADVVSHSLEARRRIGTLPHGSGLYPHLTARENIAYYGRLY
                     GIEPGQLPEKVDRVIRQLDIADFADRRTKGFSQGQRTKVALARALVHEPRNVILDEPS
                     NGLDVMATRSLRELIRQLKAEGKCVLFSSHVMQEVAALCDQIVILAGGKVSLHDSPEG
                     IRNATGCDDLEDAFVQAIDFVGGQRR"
     gene            1105..2286
                     /locus_tag="VFY27_12005"
     CDS             1105..2286
                     /locus_tag="VFY27_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766286.1"
                     /translation="MFETLRIVFGKEVLDNFRDRRTLLSALLLGPLFGPVIFAFAINL
                     SLKQSLSDASEALDLPVLGTEHSPNLVSYLRSHNINIVEAPATYEEARVAVEAGMHDV
                     VVVIDGGFGDDLLEGTPATVAVITDQANRQADRESRRAMQALNAYSDELAGIRLAARG
                     VNPMVLRPLNIDLIDVSTPSGRSALLLGMLSYFFIFALLTGGMNLAIDATAGERERGS
                     LEPLLCLPVRRDRLIYGKIAATCLFMAISLGISLVSFYVTLKFVPLERLGMTPNFGPA
                     VVGTAFLLLLPFTLLGASLMTLVASFTKSYKEAQTWVSIVLLAPTLPILIVSLLMVRP
                     STILMLVPSLSQHLLLVGLIRNEPLDFLHVVVSVVGTLVAGAVLAWVCARLYRREGLL
                     G"
     gene            complement(2375..2956)
                     /locus_tag="VFY27_12010"
     CDS             complement(2375..2956)
                     /locus_tag="VFY27_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5766287.1"
                     /translation="MSRTRVFRLFLLSLLASSATAVADGPDYSYIQGSYGQVEVDDVD
                     VDGDGFGISGSAALTERFHLFGGYTTADMGSGVDLNQLEAGVGYNHPVSDTVDVVLSL
                     AYLSAEIDLAGVGSIDDSGYGMGIGLRGMLTPEFEVNGELQYVDFGDGGDDTGFGGGF
                     LYSFTDQFAAGASGDWTGDISTYQLNARFLFGN"
     gene            complement(3090..4475)
                     /gene="mnmE"
                     /locus_tag="VFY27_12015"
     CDS             complement(3090..4475)
                     /gene="mnmE"
                     /locus_tag="VFY27_12015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5766288.1"
                     /translation="MSGTEDHGDTIVARATPAGTGGIGIVRVSGPFAPRIATSMLGRL
                     PLPRVASFSDFRDLDGELLDQGIALYFPSPASFTGEDVLELHGHGGPVVVAGLVDAAV
                     ALGARMALPGEFSRRAFANGKLDLAQVEAIADLVSSGSTQAARAAMRSLSGAFSNAVE
                     QLQERLTHLRMHVEAAIDFPEEEIDFLADDALLARVEDCAAAFRRLRDHAGVGRVLRD
                     GYRVVIVGPPNAGKSSLMNRLSGHESSIVTELAGTTRDIVREQINIDGLAVELSDTAG
                     LRDDPGVIEAEGIRRARAAMRQADAVLWIQDASTAPPAGPATAAGLMAGDDLPEPLPD
                     NIPVVIVRNKVDVSGDAPGLLRRAPTVVAISAVTGAGIDALRAELRDLAGYRDLGEGV
                     FTARRRHVDALDRAMAHFNTGRRHLGGRAAGELFAEELSLAQLALSEITGAFSSDELL
                     GRIFAEFCIGK"
     gene            complement(4540..>5025)
                     /gene="yidC"
                     /locus_tag="VFY27_12020"
     CDS             complement(4540..>5025)
                     /gene="yidC"
                     /locus_tag="VFY27_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5766289.1"
                     /translation="DRQQLSQAMMDLYKREKVNPAAGCLPILIQMPFFLAFYWVLLES
                     VEMRQAPFALWITDLSSRDPFFILPLIMGAAMFFQQKLNPAPADPVQAKVMQIMPIVF
                     TGFFAFFPSGLVLYWVTNTLLSIAQQWHINKVVGRESAKRKSGRKEGKKKEAKTKTGA
                     D"
BASE COUNT          946 a         1582 c         1546 g          951 t
ORIGIN      
        1 aactcgagag cacctacatg ggcgcggtgc tgctcgacgg gatggaagcc atctgctcgg
       61 tctggccgcg cggcgtcctt gatgccgacc tgcgcgaccc gcttgcgacc gataaacccg
      121 tgttgctgct gtcgggcgaa gccgaccccg ttactccgcc gcagttcgcc gaaatggcct
      181 tgctcgagat gacgaacagc cttcacctcg tcggtgccga ccaggggcat ggccaggccg
      241 gcaggacctg catccccgag atcatggcgc agttcgtcga gacggcgagc gtcgagggcc
      301 tggatgagga atgcctggcg gagcgccagt tcgcaatgcc gttttttctc gactacaacg
      361 gcccgtcacc atgatccggg tcagcaacct gaaaaagtcc ttcggcgaag tgaaagcgat
      421 agacggtgtc agtttcgagg cgccggacgg cctcatcacg ggcttgctgg ggccgaatgg
      481 cgccggcaag tcaaccacgt tgcgggtcct gtacacggtg ctgaaacctg atgaaggcaa
      541 cgccgacata gacggagccg acgtcgtcag ccacagcctg gaggcgcgcc ggcggatcgg
      601 caccctgccg cacggttcgg gtctctatcc acacctcacg gcaagggaga atatcgccta
      661 ctacggcagg ctatacggca tcgagcccgg gcagcttccg gaaaaggttg acagggtaat
      721 tcgccagctc gacatcgcgg acttcgcgga tcgccgcacg aaaggtttct cgcaggggca
      781 gcgcaccaag gtcgcgctgg cgcgagcgct ggttcacgag ccgcgcaacg tcattctcga
      841 tgagccgagc aacgggctcg acgtcatggc cacgcgctcg ctgcgcgagc ttatccggca
      901 actgaaagcg gaaggaaaat gcgtactgtt ttccagtcac gtgatgcagg aggttgcggc
      961 cctgtgcgac cagatcgtca tccttgccgg cggcaaggtg tccctccatg acagcccgga
     1021 aggtattcgc aacgccaccg gctgcgatga cctggaagac gcgttcgtgc aggcaatcga
     1081 cttcgtgggc gggcaacgac gctgatgttc gaaacgttgc gcattgtttt cggcaaggaa
     1141 gtgctggaca acttccgcga ccgccgcaca ctgctctccg ccttgttgct ggggccgctg
     1201 ttcgggccgg tgatcttcgc gttcgccatc aacctttcac tgaagcagtc gctgagcgac
     1261 gcgagcgagg ccctggacct gccggtgctg gggaccgagc attcgccgaa cctcgtgagt
     1321 tacctgcgaa gccacaacat caacatcgtg gaagctcccg ctacttatga agaggcaagg
     1381 gtggcggtcg aggcgggcat gcacgatgtc gtggtcgtaa tagacggggg tttcggcgac
     1441 gacctgctgg agggcacgcc cgccacggtt gccgttatca ctgaccaggc aaatcgccag
     1501 gccgatcgcg aatcgcgccg cgccatgcag gccttgaacg cgtatagcga tgaacttgcc
     1561 ggcattcgcc ttgcggcgcg cggggtcaac ccgatggtgc tgcggccgct caatatcgac
     1621 ctaatcgacg tatcgacccc cagcgggcgc tcggccttgc tgctcggaat gctcagttac
     1681 ttctttatct tcgcgctcct gaccggcggc atgaatctcg cgatcgatgc gacggccggc
     1741 gagcgtgagc ggggctcgct ggagccgctc ctgtgcctgc cggtgcggcg cgatcgcctg
     1801 atatacggaa agatagcggc cacttgcctg ttcatggcga tctcgctggg tatcagcctc
     1861 gtatcctttt acgtcacact taaattcgtg ccgctggaaa gactcgggat gacgccgaac
     1921 ttcggtcccg ccgtggtcgg cactgctttc ctgctgttgc taccgttcac gctgctggga
     1981 gcgtccttga tgaccctggt cgcctcgttt accaagagct acaaggaagc gcaaacctgg
     2041 gtcagcatcg tgctgcttgc gccgacgctg cccatcctca tcgtcagcct cttgatggtc
     2101 cgtcccagta cgattctcat gctggtaccg agccttagcc agcaccttct gctggtaggt
     2161 ctgattcgta acgaaccact ggacttcctg cacgtcgtgg tctcggtcgt gggaacgctg
     2221 gttgcaggtg cggtacttgc gtgggtttgc gcgcgcctgt accgccgcga gggcctgctc
     2281 ggttagggag cgaagaatgc gatcgggatc ggaatccccg cgcaaaaaaa accccgccct
     2341 aaaaggcggg gtttatgacg tatcccggct tccgtcagtt gccgaagagg aatctcgcgt
     2401 tcagctggta ggtgctgata tcgccggtcc agtctcccga ggctcctgcc gcaaactggt
     2461 cagtgaagct gtacaggaag cctccgccga agccggtgtc gtcgccgccg tcgccgaagt
     2521 cgacgtactg caattcccca tttacctcga actcgggcgt gagcatgccg cgaagaccga
     2581 tgcccattcc gtagccgcta tcgtcgatgg acccgacccc tgcgaggtcg atttccgcgg
     2641 acaggtacgc gagcgacagg actacatcga ccgtgtccga gaccgggtgg ttgtagccta
     2701 ccccggcttc cagctggttg aggtcgaccc ccgagcccat atccgcagtg gtatagccgc
     2761 cgaataggtg aaaacgctct gtcagggctg cagaacccga gatgccgaag ccgtcgccgt
     2821 ccacgtcgac gtcgtcaacc tccacctggc cgtaagagcc ctgaatgtag ctgtaatccg
     2881 gcccgtcggc gacggctgtc gcggaagagg caagcaggct caacaggaac aaccgaaata
     2941 cccgtgtacg agacatgtac gatctcctca tgtgtcaggg cgaaatgggc cccggagacg
     3001 gcagcttaga cgtgaggtca caggcgcgat cgcgacccgt gtcacaaacc acacgctgtt
     3061 gtacgggtac aacgattatc tgtgaaccat tacttaccga tacagaattc cgcgaaaata
     3121 cgaccgagga gctcgtcgct gctgaatgct ccggtgatct cgctcaaggc cagttgcgca
     3181 agggagagct cctctgcgaa aagctccccg gccgctcttc ccccgaggtg acggcggcca
     3241 gtattgaagt gcgccatggc gcggtccagc gcatcgacat gtcttcgcct cgcggtaaaa
     3301 acgccctcgc ccaggtcgcg gtaacccgcc agatcgcgta gctcggcacg caacgcgtca
     3361 atgccggcgc cggtcaccgc ggagatcgca acgaccgtcg gcgctcgccg cagcaggccc
     3421 ggcgcatctc cgctcacatc gaccttgttg cgcacgatga caacggggat gttgtcgggc
     3481 agcggctcag gcaggtcgtc gccggccatc aggcccgctg cggtggcagg gccggccggg
     3541 ggagccgtgc ttgcatcctg gatccagaga acggcgtccg cctggcgcat cgctgcgcgg
     3601 gcacgccgaa ttccttccgc ttctatgaca ccggggtcgt ccctcaaccc ggcggtgtcc
     3661 gaaagctcta ccgccagtcc gtcgatattt atctgctcgc gcacgatatc ccgcgtcgtg
     3721 cccgctaact cggttacgat cgaggactcg tggccgctaa ggcggttcat caggctcgac
     3781 ttgccggcgt tgggtggccc gacgataacc acccggtagc catcgcggag caccctgcca
     3841 acgcccgcgt ggtcgcgcag gcgccggaag gcggcagcgc aatcttcgac gcgcgcgaga
     3901 agcgcatcgt ccgccaggaa atcgatctcc tcttccggaa agtcgatagc cgcctcgaca
     3961 tgcatcctta agtgggtgag cctctcctga agctgttcga cggcgttcga aaatgcaccg
     4021 gagagtgagc gcatagccgc ccttgcggcc tgggtcgagc cggaagatac gaggtcagcg
     4081 atcgcctcta cttgtgcaag atccagtttg ccattggcaa acgcgcgtct cgagaattct
     4141 cccggtagcg ccatgcgcgc tcccagcgcc accgcagcgt cgaccagtcc cgcgacgacg
     4201 acggggccac cgtggccatg caattccagc acgtcttcac cggtgaacga tgcgggcgaa
     4261 ggaaagtaca gggcaatgcc ctggtccagc agctctccat ccagatcgcg aaagtcgctg
     4321 aagcttgcga ccctcggcag cgggagacgg ccaagcatgc tggtcgcgat ccgcggcgcg
     4381 aacggacccg agacgcgcac gatgccgatg ccgccggtgc ctgccggcgt cgctcgcgcg
     4441 acaatggtgt cgccgtggtc ttctgttccg ctcatggttt gccgaggctc cctgatgggg
     4501 tccaggagtt tgcgcggcag gaagatcttc gcaccagcct cagtcggcgc cggttttagt
     4561 cttcgcttct tttttcttgc cttccttcct gccggacttt cgcttcgcac tttcccgtcc
     4621 gactacctta ttgatatgcc actgctgtgc gatggaaaga agcgtattgg tcacccagta
     4681 gagcaccagt ccagacggga agaatgcgaa aaagccggtg aacacgatcg gcatgatctg
     4741 cattaccttc gcctgcacgg gatcggccgg tgccgggttc agcttttgct ggaagaacat
     4801 ggcggcaccc atgatcaggg gcaggatgaa aaaaggatct cgcgacgaga ggtccgttat
     4861 ccagagcgca aacggggcct ggcgcatctc gacgctttcg agcagtaccc agtaaaacgc
     4921 caggaagaat ggcatctgga tcaggatcgg cagacatcct gccgccgggt ttaccttctc
     4981 ccgcttgtac aggtccatca tcgcctggct cagctgctgg cgatc
//
LOCUS       DASUOM010000577         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_922187,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000577 DASUOM010000000
VERSION     DASUOM010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922187"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VFY27_12025"
     CDS             <1..1554
                     /locus_tag="VFY27_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX5766290.1"
                     /translation="FPPEPNGYLHFGHAKSIALNFGLAQQYGGRCNLRFDDTNTEKEE
                     QEYVDSILEMVQWLGYDYGTALYASDYFDTMYRAAELLISEGHAYVDSQTPEEVQLNR
                     GSFTEPGKNSPYRERNIDDNLELLREMRDGKHADGAHVLRAKIDMASPNINLRDPTLY
                     RIKRARHHRTGDQWCLYPLYTYAHPIEDAIEGITHSLCTLEFEDQRPFYDWLLNRLAD
                     GGLFKRSLPQQIEFARVNLTHVVLSKRKLIQLVEEGHVDGWDDPRMPTLAGARRRGYT
                     PEGFKLFAERIGVSKANQTIEFSVLEDCMREHLNEVAPRRMAVLDPVKLVIENYPDGQ
                     EEMMEIANHPQKPEMGSRRVPFSKELWIEREDYQEVPAKGYFRMFPGNTVRLRHGYVV
                     KCVGYDKAADTVRCEYFPDSRSGTQGAAKYKVKGNIHWVSVKHAFTAGIRTYDRLFGS
                     EHPQGIEDLNPASKVTSMAHVEPSLQSPEEPTYQFERHGYYTLDSSATRTFNRTVTLR
                     DSWAGGKPA"
     gene            complement(1514..2065)
                     /locus_tag="VFY27_12030"
     CDS             complement(1514..2065)
                     /locus_tag="VFY27_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX5766291.1"
                     /translation="MALKATIFKASLQVSDIDRNCYGEHQLTLARHPSETDERMMMRV
                     LAFALNAHEHLQFGKGLSDQDEPDLWQKDLTGSIVHWIDVGQPDEKRLIRASGKADRV
                     TVYTYGRGAEMWWEANAGRLARARNLAVWRVPGDESLALAQLADRTMRLQCTVQEDQV
                     WFSGPADTLPLKPVCLQPRSPAG"
BASE COUNT          427 a          715 c          708 g          386 t
ORIGIN      
        1 tttccgccgg agcccaacgg ctatctgcat ttcgggcacg ccaagtccat cgccctgaat
       61 ttcgggctgg cgcagcagta cggcgggaga tgcaacctgc gcttcgacga caccaatacg
      121 gagaaggagg agcaggaata cgtcgattcg atcctcgaga tggtgcagtg gctcggctac
      181 gactacggca ccgcgctgta cgcatcggat tacttcgaca ccatgtaccg ggcggcggag
      241 ctgctgatct ccgagggcca cgcctacgtg gacagccaga cgccggagga agtccagctc
      301 aaccggggct ctttcaccga gccggggaag aacagtccct accgggagcg caacatcgac
      361 gacaacctgg agctgttgcg cgaaatgcgc gacggcaagc acgccgacgg cgcccatgtg
      421 ctgcgcgcga aaatcgacat ggcttcgccc aacatcaacc tgcgcgaccc gacgctctac
      481 cggatcaagc gcgccaggca ccaccgtacc ggggaccagt ggtgcctcta tccgctgtac
      541 acctacgcgc atccgatcga ggacgcgatc gagggcatca cccattcgtt gtgcaccctg
      601 gaattcgagg atcagcggcc gttctacgac tggctgttga acaggctcgc cgacggcggg
      661 ctgttcaagc gctccctgcc ccagcagatc gagttcgcgc gcgtcaacct gacccacgtc
      721 gtgctgtcca agcggaaatt gattcaactg gtcgaggaag gccacgtgga cggctgggac
      781 gatccgcgca tgccgacgct ggcgggcgcg cggcgccgcg gctacacgcc ggaaggcttc
      841 aagctgttcg ccgaacgcat cggcgtgtcc aaggccaacc agaccatcga gttttccgtg
      901 ctcgaggatt gcatgcgcga gcacctgaac gaggtcgcgc cgcggcgcat ggcagtgctc
      961 gatcccgtga agctggtgat cgagaattat ccggacggac aggaagagat gatggagatc
     1021 gcgaaccatc cgcagaagcc ggagatgggc tcgcggcgcg tgccgttttc gaaagagcta
     1081 tggatcgaac gggaggacta ccaggaagtg ccggcgaagg ggtacttcag gatgtttccg
     1141 ggaaacacgg tgcggttacg gcatgggtac gtcgtcaaat gcgtgggcta cgacaaagcg
     1201 gcggatacgg tgcgctgcga atactttccg gattccaggt caggcacgca aggcgcagcg
     1261 aagtacaagg tgaaggggaa catccactgg gtgagcgtga agcacgcttt tacggcgggg
     1321 atacggacct atgatcgctt gttcgggagc gagcatccgc aggggatcga ggacttgaac
     1381 ccggcgtcga aggtgacgtc gatggcgcac gtcgagccga gcctgcagag cccggaagag
     1441 ccgacctacc agttcgagcg gcacgggtac tacacgctgg atagcagcgc gacgcgcacc
     1501 ttcaatcgca cggttaccct gcgggactcc tgggctggag gcaaaccggc ttgagcggca
     1561 gcgtgtccgc ggggccgctg aaccacacct gatcctcctg caccgtgcac tgcaaccgca
     1621 tggtgcgatc ggccagctgc gccagcgcca ggctctcgtc gccgggcacg cgccacacgg
     1681 caaggttcct cgcacgcgcc agtcgcccgg cgttcgcctc ccaccacatc tccgctcccc
     1741 tgccgtacgt atagaccgtg acccggtcgg ccttcccgct cgctcgaatc agccgctttt
     1801 catccggctg ccccacgtcg atccagtgca cgatcgatcc cgtcaggtcc ttctgccaga
     1861 ggtccggctc atcctggtct gacaatccct tgccgaactg aaggtgctca tgcgcgttca
     1921 acgcgaacgc cagcacgcgc atcatcatgc gttcgtcggt ctccgaagga tgccgggcga
     1981 gcgtgagctg gtgctcgccg tagcagttcc ggtcgatgtc cgaaacctgc aggctcgcct
     2041 tgaagatggt ggccttgagt gccatggcgg cacgctaacg tcagtccgcg ctgtcgggtg
     2101 cgcccgcggc cgatgccagg tcttcctgct tcaccttctc cgtgttgaac ttccccgccc
     2161 gctgcgtcat ggtcttcagc acttcctcga gctcgggcgg ctcgcccttg atgaagcgca
     2221 gcggattgat gcgcgc
//
LOCUS       DASUOM010000578         7453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_801426,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000578 DASUOM010000000
VERSION     DASUOM010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801426"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VFY27_12035"
     CDS             <1..1018
                     /locus_tag="VFY27_12035"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5766292.1"
                     /translation="LKPGFLIGGVPGNFNESARFGDSQLFVVEADEYDTAFFDKRAKF
                     VHYRPRTVVLNNLEFDHADIYKNVDEIIWQFHQLLRTVPGNGKVIMNATDANLQAVIN
                     RGCWTPVESFGLSSGDWSGEFLDAAERHLAIHGPGGSRWETLWSLGGLHNLENALAAV
                     AAASSVGVPPEAAVASLSRFEGVKRRMERTATVGDVPIYDDFAHHPTAIRRSLESLGK
                     RYPGRRIVVALEPRSNTMKLGVHNDTLGESLAAADIAFVFCPEEVGPGLEQGLRKLAD
                     RTRLFADYDSLVEGIVKDAQPRDVLVFMSNGGFGGVRQKVTSALQQARDAAVVSPGAA
                     RSIT"
     gene            1099..1680
                     /locus_tag="VFY27_12040"
     CDS             1099..1680
                     /locus_tag="VFY27_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5766293.1"
                     /translation="MQVPLFPLRTVLYPKGPLPLRIFEPRYLDMVSECLKNDRPFGVL
                     LIRAGSETGPAITYSTGTLAKIIDWYQGSDGLLGITAVGQQRFRLLHTERRKNNLDVG
                     RIRILPENKNVPLPEEMKPLAKVLESVLDDLGSLYGAVERRYDDAGWVACRFAEILPI
                     APEVKQACLELDDPVERLNAVRKLIEDVRDTEL"
     gene            complement(1730..2998)
                     /gene="hemL"
                     /locus_tag="VFY27_12045"
     CDS             complement(1730..2998)
                     /gene="hemL"
                     /locus_tag="VFY27_12045"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5766294.1"
                     /translation="MTLIEDARKYIAGGVNSPVRAFTAVGGEPVFIRRAMGAWLETED
                     GRKLVDYVGSWGPMILGHAHPAVVKAVREAAAEGLSFGAPTVRETELARKICEFVPSV
                     ERLRMVNSGTEATMSAIRLARGFTGRDKIVKFEGCYHGHSDSLLIKAGSGALTLGVPS
                     SAGVPAALAEHTLTLEYNSIGDVQKAFTELGSDIAAVIVEPVAGNMNLVLPEADFIST
                     LRECCDVHGSLLIFDEVMTGFRVAQGGAQQLFGIRPDLTTLGKIIGGGMPAAAFGGRA
                     DIMALLAPDGPVYQAGTLSGNPLAMAAGLATLRELEQPGFHEALSEKTRGLVDGLQAM
                     ARETGIPIAAEACGGMFGFVFSEDAPMRRLSQVAAGDIARFRKFFHGMLQQGVYFAPS
                     AFEAGFVSAAHGEDEIAFTLEAARKVFADL"
     gene            3109..3273
                     /locus_tag="VFY27_12050"
     CDS             3109..3273
                     /locus_tag="VFY27_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX5766295.1"
                     /translation="MKTYMCLICGFIFDEAEGDPGGGIPPGTRWEDVPLAWRCPDCGA
                     GKEDFEMVEV"
     gene            complement(3318..4235)
                     /locus_tag="VFY27_12055"
     CDS             complement(3318..4235)
                     /locus_tag="VFY27_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5766296.1"
                     /translation="MKRQTPSFATRWASAGWLLLVLSLAATAEDAGHPVSMWQLDGHA
                     NSVYLLGSVHLLRADDHPLPSVIDEAYEDAEKVIMELDMDDIDPVEAQSLLTELGLIH
                     DDRSLSDLMGPEPYAEAAALASSADIPLAMLVKSEPWLAAITVEQLMLSRIGFNPLYG
                     IEHHFMEKAEADGKEILGLETVEEQLKFLDSMSLESQRALLLQSLKESVDIRKIMDEL
                     VDAWRHGDIAFMQDNMLDEMKQYPELYRTIVVDRNRKWLGKVQELLDDDDDYLIVVGA
                     LHLIGEDGLPDLLAEHGLEVRQLHQQPPD"
     gene            complement(4259..5017)
                     /locus_tag="VFY27_12060"
     CDS             complement(4259..5017)
                     /locus_tag="VFY27_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5766297.1"
                     /translation="MHPVDPVRVLHFTDPHLFATADGSLRGRVTHATLQRVLEHYRKS
                     GWSADHVQVTGDIIQDDTREAYVRFRETVRPIGLPVYCVPGNHDIRGMMREELQDEQF
                     HYCSSLEANNWLIAGIDSSMEGQAAGSLDSSELERLDTLVNNTGAEHVMICLHHPPLP
                     VGSRWLDELALQNAEEFLEHANASGKVRLVVFGHVHQAFDDVYHSLRIIGTPSTCRQF
                     AQDSDTFAVDQKPPAYRRIELYARGGVETEVVWA"
     gene            complement(5057..6370)
                     /gene="ahcY"
                     /locus_tag="VFY27_12065"
     CDS             complement(5057..6370)
                     /gene="ahcY"
                     /locus_tag="VFY27_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5766298.1"
                     /translation="MSSKAVALKQQDYKVADISLAGWGRKEIAIAETEMPGLMALRAE
                     YAGKTPLAGAHVTGCLHMTIQTAVLIETLTALGAEVRWSSCNIFSTQDHAAAAIAASG
                     VPVFAWKGETEEEYWWCIEQTINGPDGWRPNLILDDGGDLTLVMHDKHPEAMKGVVGL
                     SEETTTGVKRLYDMQKEGTLRCPAFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMI
                     AGKIAIVCGYGDVGKGCAQSLRGLGATVWITEIDPICALQAAMEGYRVVTLEDASDKA
                     DIIVTATGNYHVLTGAHMERMKNQSIVCNIGHFDNEIDVAYLKQYEWENIKPQVDHVV
                     FPDGKRIILLAEGRLVNLGCGTGHPSFVMSNSFTNQVLAQVELWQNHAKYENKVYVLP
                     KHLDERVARLHLERVGAKLTEMTDEQAKYIGVKKDGPYKSDHYRY"
     gene            complement(6485..>7453)
                     /gene="metK"
                     /locus_tag="VFY27_12070"
     CDS             complement(6485..>7453)
                     /gene="metK"
                     /locus_tag="VFY27_12070"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5766299.1"
                     /translation="DIEALVRETVIGIGYSDSAMGFDGASCAVITALGKQSPHIRQGV
                     DRGDPENQGAGDQGLMFGYACNETSVLMPAPITYAHRLVRRQAEVRKAGTLPWLRPDA
                     KSQVTFVYEDDKPVAIDAVVLSSQHHPDVSTAELTDGVMEEIIKPVLPGKWISKKTQF
                     HINPTGRFEIGGPMGDCGLTGRTIIVDTYGGSARHGGGAFSGKDPSKVDRSAAYACRY
                     VAKNIVAAGLADRCEIQVSYAIGVAEPTSISVQTFGTGRISDDRLVDLIRENFDLRPY
                     GILKMLDLIRPIYKPTAAYGHFGREDLELSWERSDKAETLRGAVAA"
BASE COUNT         1373 a         2319 c         2253 g         1508 t
ORIGIN      
        1 attgaagccc gggttcctga ttggcggagt tccgggcaac ttcaatgaat ctgcgcgttt
       61 cggcgattcg cagttgttcg tcgtcgaagc cgatgaatac gacaccgctt ttttcgacaa
      121 gcgggcgaag ttcgtgcact accggccacg taccgtggtt ctgaacaatc tcgaattcga
      181 tcacgccgac atctataaga acgtcgacga gatcatctgg caattccacc agttgcttcg
      241 caccgtgccg ggaaacggca aggtcatcat gaacgcgacc gacgcaaact tgcaggccgt
      301 catcaacaga ggttgctgga cgccggttga aagctttggt ctttcttccg gcgactggag
      361 tggcgagttt cttgatgctg cggaaaggca cctcgccatc cacggacccg gaggcagccg
      421 gtgggaaacg ctgtggagcc tgggcggcct gcacaacctg gagaacgccc tggccgcagt
      481 cgcggcggcg agttcggtcg gtgtgccgcc tgaagccgcg gtcgcttcac tgtcacgttt
      541 cgagggggtg aaacgccgca tggagcgcac ggcgaccgtg ggcgacgttc ccatatacga
      601 tgattttgcg caccacccga cggcgatccg gcgctcgctg gaatccttgg gcaaacgcta
      661 tcccggccgc cgcatagtgg ttgcgctcga gcctcgctcc aacacgatga aactcggtgt
      721 gcacaatgac actctcggcg agtcgcttgc cgctgccgat atcgcgttcg tcttctgccc
      781 ggaagaggtc gggccgggac tggaacaagg cctccgcaag ctggccgatc gcaccaggtt
      841 gttcgccgac tacgattcgc tcgtcgaagg cattgtgaaa gatgcgcagc cgcgggacgt
      901 gctggtgttc atgagcaatg gcggattcgg cggcgtgcgc caaaaggtaa cgtcggcgct
      961 gcagcaggcg cgcgacgcag cggttgtgtc gcccggcgcc gcacgttcta taacctgaga
     1021 ctggcgggcc tgccccggcg gcggcgcaag gtaacgactc atcgggttgt gcgcatcgaa
     1081 ccaggagcag aggaagcgat gcaggttccg ctctttccat tacgcaccgt gttgtaccct
     1141 aaaggaccgt tgccactgcg gatattcgag ccgcggtacc tggacatggt cagtgaatgc
     1201 ctgaagaacg accggccatt cggcgtgcta ttgatacgtg cgggaagcga aaccggaccg
     1261 gcaatcacct acagcacagg aaccctggca aagatcatcg actggtacca gggcagcgac
     1321 ggcctgctcg gcattaccgc ggtcggccag cagcgtttcc ggctccttca caccgagcgc
     1381 cgcaagaaca accttgatgt cgggcggatc aggatcctgc cggagaacaa aaacgtgcca
     1441 ttgcccgagg aaatgaaacc cctcgccaag gttctcgaga gcgttctgga tgatctcggc
     1501 agcctttatg gagccgtcga acgccgctac gacgatgccg gttgggttgc ctgccgcttt
     1561 gccgaaatcc tgccgatcgc cccggaagtc aagcaggcct gccttgaact tgacgatccg
     1621 gttgagcgac tgaacgccgt ccgcaagctg atcgaggacg tacgggatac agaactgtag
     1681 gagtccgcca tggggggcga ttagataagg gctggcgctc accacccaat cagaggtcgg
     1741 caaacacctt tcgcgcggcc tcgagagtga acgcaatctc gtcttcgccg tgcgcggctg
     1801 aaacgaaacc ggcttcgaag gccgacggag caaaatatac cccctgctgc agcatcccgt
     1861 gaaagaattt gcggaagcgg gcgatgtcgc ccgctgcgac ctgggagagt cggcgcatcg
     1921 gtgcgtcttc gctgaagacg aaaccgaaca tgccgccgca ggcttcagct gcgatcggga
     1981 tgccggtctc acgcgccatg gcctgcaggc catcgaccag ccccctggtt ttttccgaca
     2041 gcgcttcatg gaagcccggt tgctcgagtt cccgcagcgt cgcgagcccc gctgccatcg
     2101 ccagtggatt gccggagagg gtgcccgcct ggtacaccgg cccgtcggga gcgagcaacg
     2161 ccattatgtc ggcacggccg ccgaaagcgg ctgcgggcat tccgcccccg atgatcttgc
     2221 cgagtgtcgt caggtcgggc cgtatgccga agagttgctg ggcgcctccc tgggcgacgc
     2281 ggaatccggt catgacttca tcgaaaatca gcaacgatcc gtgtacgtca cagcactcgc
     2341 gcagagtgga gatgaaatcc gcctccggca ggacgaggtt catattcccg gccaccggct
     2401 ccacgatgac cgccgcgata tcggacccca gttcggtaaa ggccttttgc acgtctccga
     2461 tactgttgta ttccagcgtg agcgtgtgct ccgcaagggc ggccgggaca ccggcggaac
     2521 tcggcacgcc aagagtgagc gcgccggagc cggccttgat gagcaacgag tcggagtgcc
     2581 cgtgatagca gccttcgaac ttgacgatct tgtcgcggcc cgtgaacccg cgcgccagcc
     2641 ggatggcgct catggtggct tcagtccccg aattgaccat tcgcagacgc tcgaccgacg
     2701 gcacgaactc gcagattttt ctcgccagtt ctgtctcgcg aaccgtggga gcgccgaagc
     2761 tcaagccctc cgccgccgct tcgcgtaccg ccttcacgac tgcggggtgt gcatgcccca
     2821 ggatcatcgg accccacgac ccgacgtagt ccacgagctt ccggccatct tccgtttcca
     2881 gccaggctcc catggcacgc cggatgaaaa cgggctctcc gccgaccgct gtaaaagcgc
     2941 ggacgggcga gttgacgcct ccggcgatgt acttcctggc gtcctcgatg agcgtcatac
     3001 gtcctttccg tgcagttctc acgcatgcta cccaagcgca ggtgagcgcg aaaccccgca
     3061 cctcatcctg taaaatgcgg acctttatcc gaaccggacc agcgcttgat gaaaacctac
     3121 atgtgcctga tttgcgggtt tatcttcgac gaagcagaag gcgacccggg aggcggcatt
     3181 ccccccggta cgcgctggga ggatgtgccc ctggcatggc gctgcccgga ctgcggggcg
     3241 ggcaaggaag atttcgagat ggtcgaggtt tgactgtgtc aaggcatttg caccgatcat
     3301 gccttttgca gcggctccta atccggcggc tgttggtgaa gctggcgtac ctccaggccg
     3361 tgttccgcga gtaagtcagg cagtccgtcc tcgccgatga ggtgcagcgc cccaacgacg
     3421 atgaggtagt cgtcatcatc gtccagcagt tcctgcacct tacccagcca cttccggttg
     3481 cgatcgacga caatcgtccg gtacagctcc gggtactgct tcatctcgtc gagcatgttg
     3541 tcctgcatga aggcaatgtc gccatgtcgc caggcatcga cgagttcgtc catgatcttc
     3601 cggatgtcga cactttcctt gagcgactgc aacagcaggg cgcgctgcga ttccagcgac
     3661 atgctgtcca ggaacttgag ttgctcctcg acggtttcaa ggccgaggat ttcctttccg
     3721 tcggcctcgg ctttttccat gaagtgatgc tcgatgccat agagcgggtt gaagccgatg
     3781 cgcgaaagca tcagctgctc tacggtaatg gcggcgagcc agggctctga tttcacgagc
     3841 atcgccagcg gtatgtcggc gctactcgcc agcgctgcgg cttccgcata tggctcggga
     3901 cccatcaggt cgctgaggct cctgtcatcg tggatgaggc caagttccgt caacagcgac
     3961 tgggcttcga ccgggtcgat gtcatccatg tccagctcca tgatgacctt ctcggcatct
     4021 tcataggcct cgtcgatcac ggatggcagc ggatgatcgt cggcgcggag caggtgcacg
     4081 gaaccgagca ggtagacgct gttagcatgc ccgtccagct gccacatgga aacaggatgg
     4141 cctgcgtcct cggccgtcgc ggccagggaa agaacgagca gtagccagcc ggcgctcgcc
     4201 catcgtgtcg cgaatgaggg cgtctgtctt ttcatgttat gcatgtgcga acgcctcttc
     4261 aggcccagac cacttccgtc tccacccctc cgcgtgcata cagttcgatg cgacggtagg
     4321 ccggcggctt ttggtctaca gcgaaggtgt cgctgtcctg cgcgaattgc cggcaggtcg
     4381 atggtgtgcc gataatcctc aacgagtggt acacgtcgtc gaaagcctgg tgcacgtgcc
     4441 cgaacacaac cagcctgacc ttccccgaag cgttagcatg ctccagaaac tcttccgcgt
     4501 tctgcaatgc cagctcatcc agccacctgc tgccaaccgg caagggcggg tggtgcaggc
     4561 aaatcatgac gtgctcggcg ccggtgttgt tcacgagtgt gtccaggcgt tctaattcgc
     4621 ttgagtcgag actgccggct gcctgcccct ccatgctgct gtcgatcccg gcgatgagcc
     4681 agttgttggc ttcgagagaa ctgcagtaat gaaactgctc gtcctgcagc tcttcgcgca
     4741 tcatgcctcg aatgtcatgg ttgcccggca cgcaataaac cggcaggccg atcggcctga
     4801 ccgtttcgcg aaaacgtacg tacgcctcgc gcgtgtcgtc ctggatgatg tcgccggtca
     4861 cctggacgtg gtctgctgac cagcccgact tccggtaatg ttccagcacc cgttgcaggg
     4921 ttgcgtgggt aacccgcccg cgcaagcttc catccgctgt cgcgaacaaa tgcgggtccg
     4981 tgaagtgcag gacccgaacc ggatcgacgg ggtgcactga aatgcgtcag cctgcgttca
     5041 aaggaaacat gtgctgtcaa taacgatagt gatcggactt gtacggcccg tccttcttca
     5101 caccgatgta tttagcctgc tcgtcggtca tttcggtcag cttcgcaccg acccgctcga
     5161 ggtgcaggcg tgccacacgc tcgtcgagat gcttcggcag cacgtacacc ttgttctcgt
     5221 atttcgcgtg gttctgccag agttcgacct gtgccaacac ctggttcgtg aacgagttcg
     5281 acatgacgaa actcgggtgg cccgtcccgc agcccaggtt cacgagcctg ccttccgcaa
     5341 gcaggatgat ccgcttgccg tcggggaaga ccacgtggtc cacctgcggc ttgatgtttt
     5401 cccactcgta ctgcttcagg tatgcaacgt cgatctcgtt gtcgaagtga ccgatgttgc
     5461 acacgattga ctggttcttc atccgctcca tgtgagcgcc ggtgaggacg tgatagttgc
     5521 cagtagccgt gacgataatg tccgccttgt cgctggcatc ctcgagagta acgacgcgat
     5581 agccttccat tgcagcctgc agtgcgcaga tcgggtcgat ctccgtgatc catacggttg
     5641 cgccgaggcc gcgcagggac tgcgcgcagc ctttgccgac atcaccatag ccgcagacga
     5701 tagcgatttt cccggcgatc atgacgtccg tggcgcgctt gatgccgtca acgagcgact
     5761 cgcggcagcc gtacaggttg tcgaacttgg acttcgtcac ggaatcgttc acattgaacg
     5821 cggggcaccg caacgttccc tctttctgca tgtcgtacag gcgcttgacg ccggtagtgg
     5881 tttcctctga caggcccacg acgcccttca tcgcctccgg gtgcttgtcg tgcatgacca
     5941 gcgtgaggtc gccgccgtca tcgaggatca ggttcggccg ccagccgtcc ggcccgttga
     6001 tggtctgctc gatgcaccac cagtactctt cctcggtctc gcctttccac gcgaataccg
     6061 ggacgccgct cgcggctatc gccgcagccg catgatcctg cgtcgagaag atattgcagg
     6121 atgaccagcg tacttcggcg cccagtgcag tgagagtttc gatcagcacc gcggtctgga
     6181 tcgtcatgtg caggcagccg gtcacgtggg ctccggccag tggagtcttg ccagcatact
     6241 cggcgcgcag cgccatcagg ccgggcattt ccgtctccgc gatggcgatt tccttgcggc
     6301 cccagccggc cagggaaatg tcagccactt tgtagtcctg ctgcttgagc gcaacggctt
     6361 tgctgctcat tggaacctcc tgattagatg caaatgttcg ggaaatgtgt cgcgcgacca
     6421 gcagtcgctg cgaatgatgc tcaacgctcg ttaccggcca ggggccggcg gtcgcagccg
     6481 tccgtcaggc ggcgacggct ccgcgtaatg tttcggcctt gtcggaacgc tcccagctca
     6541 gctcgagatc ctcgcggcca aaatgtccat aagccgccgt cggcttgtaa attggccgga
     6601 tcaggtcgag catcttcaat attccataag gcctcaggtc gaaattctcg cgtatgaggt
     6661 cgaccagccg gtcgtcgctg atccgtccgg taccgaaagt ctgcacgctg atggacgttg
     6721 gctccgcgac gccaattgcg tacgagacct ggatttcgca gcgatcggcc aggcccgccg
     6781 cgacgatgtt cttcgccacg tagcggcacg cataggcggc cgagcggtcc accttggacg
     6841 ggtccttgcc ggagaacgct cctccgccgt gacgcgcgga tccgccgtag gtatctacaa
     6901 tgatcgtccg cccggtcagt ccgcagtcac ccattgggcc gccaatctcg aaacgcccgg
     6961 tcgggttgat gtggaactgc gtcttcttgc tgatccactt tccgggcaat accggcttga
     7021 tgatctcttc catgacgccg tcggtaagtt ctgccgtact gacatccggg tggtgctggg
     7081 acgaaagtac gacggcgtcg atcgctaccg gcttgtcatc ctcgtagacg aaagtgacct
     7141 ggcttttcgc atccggccgc agccacggca gcgttccggc cttccggacc tctgcctggc
     7201 gcctcacgag gcggtgagcg taggtgatgg gtgcaggcat caggacactg gtctcgttgc
     7261 aggcgtagcc gaacatcagg ccctggtcgc cggcgccctg gttttccggg tcgccacggt
     7321 cgacgccctg gcggatgtgc ggcgattgct tgccgagcgc agtaataacg gcacacgaag
     7381 cgccgtcgaa gcccatcgcg gagtccgagt agccgatgcc gatcaccgtc tcccgcacca
     7441 gtgcctcgat gtc
//
LOCUS       DASUOM010000579         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_991039,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000579 DASUOM010000000
VERSION     DASUOM010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991039"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFY27_12075"
     CDS             complement(<1..443)
                     /locus_tag="VFY27_12075"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5766300.1"
                     /translation="MDIVLRASVMFFALYLLVRVMGKRELAQMTPFELIVLVVLGDLI
                     QQGVTHNDFSLTGAILAISTMGFWALALSWITFLSPRAEALLDGEPRVIVRDGEIVQE
                     NLDRDRLTQAEILSEMRLAGIARLSEVAWAILEPHGKMSFIKRVS"
     gene            complement(443..967)
                     /locus_tag="VFY27_12080"
     CDS             complement(443..967)
                     /locus_tag="VFY27_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766301.1"
                     /translation="MDGRARPQGIEHKHIHEAGGSIFRRWPFGLAALALVLAPAFFGV
                     YGSKATLAGAGNDVRFSVKGPQRIRNGEFFEMIVTIEAQRRIEDAVVLVGASLFRDMT
                     INTLLPDPSEHGFRSGSLEFRFGALGAGETLQVKLDGQVNPGHTPSANKGSIAVADGE
                     TTLATVSYAMEVLP"
     gene            1355..>2518
                     /locus_tag="VFY27_12085"
     CDS             1355..>2518
                     /locus_tag="VFY27_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5766302.1"
                     /translation="MSTAPGSSSIRGALPVQRRRPRAGERTRGAVDALQTKIAVNVQN
                     LDTATYQQQLRDNIAELPDATGCDAAFLALLNDDGSAIETILSSSSVFSGLNPEPLTG
                     ELLEAWPWLLGRIGHLRLIDISDTDEGPGIARPELKRLGELNIGSCLVIGFTVRNALA
                     GFLALANERPVECWDASLHLLMKLIGASLASGLERMRTLAALEELEERNRLVNLTAND
                     GIWDFDGQTKRIELSRRWKEMLGYDQDRDDVLPDWYRLVHPDDMARVQAKMREHLEGK
                     TPFFESVHRMKHQNGEWRWMTSRAKARQDDKGRLIRLLGVEVDITERKLYEEALFREK
                     ESAQITLRSIGDGVVTTDANCNVEYINPVAEELTGWKVDDASGRPIDDIFRGFH"
BASE COUNT          531 a          745 c          768 g          474 t
ORIGIN      
        1 atgctgacgc gcttgatgaa actcatcttt ccgtgtggtt cgagtattgc ccatgccact
       61 tcgctcaagc gggcaatccc cgcgagcctc atctcggaga gaatttcggc ctgggttagc
      121 cgatcccggt ccaggttttc ctggacaatc tctccgtcac gcacgattac ccgcggctcc
      181 ccatcgagca gtgcttcagc tctgggtgag aggaacgtta tccagctcag ggccagggcc
      241 cagaaaccca tcgtgctgat cgcgagaatt gcgccggtga gcgagaagtc gttgtgcgtg
      301 acgccttgtt gaatcaggtc gcccaggacg acgagcacaa tcagttcgaa aggagtcatc
      361 tgtgcgagtt ctctcttgcc catcacgcgc acgagcaggt agagcgcgaa gaacatcacg
      421 cttgcgcgga gtacgatatc catcagggaa gtacctccat tgcgtagctg accgtagcca
      481 acgtggtctc accgtctgca accgctatcg aacctttgtt ggctgacggt gtatggcccg
      541 ggttaacctg gccatccagt ttcacctgca gggtttcgcc ggcgccgagc gcgccgaagc
      601 gaaactccaa cgaaccgctg cgaaagccgt gctcggaagg atcaggcagc agggtgttta
      661 tggtcatatc acggaacagc gaggcgccaa ccaggacgac tgcatcctca atccggcgct
      721 gagcttcgat ggttacaatc atctcgaaga actcgccgtt gcggatgcgc tggggaccct
      781 tgacgctgaa ccggacgtcg ttcccggccc ccgccaaagt cgctttgctg ccgtaaacgc
      841 cgaagaatgc aggcgcgagg acgagagcga gagccgcaag gccgaatggc cagcgccgga
      901 agatcgagcc tcctgcctcg tggatgtgtt tgtgctcgat tccctgtggt cttgcccgcc
      961 cgtccataac ccctcgattg acgccatggc gcgtccgtga actggccggg catggctggt
     1021 ggaggtgcga ctagccaagc tgcagcaaga accgtgcccg ttcgacaaac gtcatgggct
     1081 gcgcgcgcat tggcaggacc aggaccaaat ccccgttcgc catttgcaaa gtctacacac
     1141 gtggtttgtc gaacgagggg ttaaaccttt actaaactgc aaaattacca tgcacttatc
     1201 ttgaattcct gtgatgacgt gtcagaatgc gcgggtaatt ggggaaacga tgggcacggg
     1261 catttgtgcg gcagtccgca ggccaggtgc tccgcgacgg atagactgtg gccttaaccg
     1321 gtacatcaag aacaaccaac tctaggcgac gagcatgtca accgcgcccg gcagcagcag
     1381 cataagggga gctttgcccg tccagcgtcg tcgaccgaga gccggcgagc ggacgcgtgg
     1441 tgcggtcgat gcgctgcaaa ccaagattgc ggtcaatgtc cagaatctcg acaccgccac
     1501 ctaccagcag caactgcgcg acaacatcgc ggaactgccg gacgccaccg gatgcgatgc
     1561 ggccttcctt gcgctgctca atgatgacgg cagcgctatt gaaactatcc tgtcatccag
     1621 ttccgttttc tccgggctca atcccgagcc cctgaccggc gagcttctgg aggcatggcc
     1681 gtggctgctg ggtcgcatcg gtcacctgcg actgatcgac atctctgaca cagacgaggg
     1741 cccgggcatt gccaggccgg aattgaaaag gcttggggag ctcaacatcg gctcgtgcct
     1801 cgtcatcggc ttcaccgtac gcaacgcact cgcgggcttt ctcgccctgg caaacgaacg
     1861 cccggtggag tgctgggatg cgagcctgca tttgctgatg aagctcattg gtgcgtcgct
     1921 cgcatcgggg ctggagcgca tgcgaacgct tgcggcgctc gaggaactgg aggaacgcaa
     1981 ccggctagtg aacctcaccg ccaacgacgg catctgggac ttcgacgggc agaccaagcg
     2041 catcgaactc tcgcggcgct ggaaggaaat gctcgggtac gaccaggacc gtgatgatgt
     2101 cctgccggac tggtatcgcc tggtgcatcc cgacgacatg gcccgcgtgc aggcgaagat
     2161 gcgcgagcac ctggagggca agacgccgtt tttcgaaagc gtgcaccgca tgaagcacca
     2221 gaatggcgag tggcgctgga tgaccagtcg cgccaaggcg cggcaggacg acaagggccg
     2281 ccttatccgc ttgctgggcg ttgaagtaga cattacggaa cgcaagcttt atgaagaggc
     2341 gctgttccgc gaaaaggaaa gcgcgcagat cacgctgcgg tcgattggcg acggggttgt
     2401 caccaccgat gcaaactgca acgtcgagta tataaacccc gtcgcggaag agctgaccgg
     2461 ctggaaagtc gacgatgcga gcggccggcc gatcgacgat atcttccgcg gttttcac
//
LOCUS       DASUOM010000580         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1310928,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000580 DASUOM010000000
VERSION     DASUOM010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310928"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..770
                     /locus_tag="VFY27_12090"
     CDS             150..770
                     /locus_tag="VFY27_12090"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5766303.1"
                     /translation="MARIVEIHPVNPQPHRIASVVGAIRDGGLIAYPTDSSYALGCQI
                     GDKKAMDRIHRIRRTDKNHNFTLVVRDLSEISVYARVDNWIYRLLKSLTPGPYTVILQ
                     ATREVPKRLQNPRRRTIGVRVPDHPIVAALLDALGEPIMSSTLHLPGDDLPLTDPHEI
                     NSRIGHEIEVVVDAGPTGIEPTTVIDLSGGAVEILRRGRGDVSFRA"
     gene            905..1705
                     /locus_tag="VFY27_12095"
     CDS             905..1705
                     /locus_tag="VFY27_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5766304.1"
                     /translation="MPFAVVDGEPVTQLPQDLYIPPYALQVFLEAFEGPLDLLLYLIR
                     RQNLDILDIPIAEITRQYVQYIELMKELHLELPGEYLLMAAMLAEIKSRMLLPRPQAE
                     EEEEDDPRAELVRRLQEYERFKKAAEELGALPRLERDVFVASVEAPERKVVAQLPDVT
                     LKELLIAFHDVLKRAEMFSRLHLRREPLSVRQRMSEILLRVKANTFSSFSDLFDPEEG
                     RMGVAVTFIAILELLREAAIEVAQAEAFAPLHVRAASSVRLVSDDGVA"
     gene            1745..2605
                     /gene="scpB"
                     /locus_tag="VFY27_12100"
     CDS             1745..2605
                     /gene="scpB"
                     /locus_tag="VFY27_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5766305.1"
                     /translation="MDQNEIKYFVEAALLAAGRPLSIDQLQNLFDRNSVPERTQIREA
                     ISGLQEDYEERGLAISEVASGFRTQIKASMADRLQKLWEERPPRYSRAFFETLALIAY
                     RQPMTRGEIEEIRGVAVSANIMRSLLERDWIRVVGHRDVPGRPAMFGTTRHFLDYFGL
                     KKLDDLPPLADLADWETLRVQLNLPEVSDEEAEPETAEIADLPVLLYPQSYQADDEAG
                     AASPGEDEASAEDIADAAATAGFEAIPPTRWPGPVEILRVVPGDDIEDPAEDEAGKVL
                     KDRDALISRR"
     gene            2626..3435
                     /locus_tag="VFY27_12105"
     CDS             2626..3435
                     /locus_tag="VFY27_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5766306.1"
                     /translation="MSERIQKVLAAAGHGSRRQIEGWIRESRLRVDGRVATLGESVSG
                     SETITLDGRSLAVRPAAGAHRHIIYNKPGDEITSRSDPEGRRVVFDSLPKLSGSRWVA
                     VGRLDMATTGLLIFTTDGDLANALMHPSSELVRRYAVRVHGAPSQADVTRLLEGVELE
                     EGGRAAFDSIERSGGDGANRWFTVTLREGRNREVRKMWAAVGYEVSRLIRTAYGPLEL
                     PRSLRRGKYVALTPAQVRTLYCSAGLDPPRPASMSPASRGRRPRHARAKSR"
     gene            complement(3471..>3732)
                     /locus_tag="VFY27_12110"
     CDS             complement(3471..>3732)
                     /locus_tag="VFY27_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5766307.1"
                     /translation="RPYWGAYSVSKFGIEALTQILAAENKHVPIRVNCVNPGPTRTEL
                     RLQAYPAEEKSASKPPTEVLSPYLFLLGPESREVTGMSLDSQ"
BASE COUNT          771 a         1122 c         1151 g          688 t
ORIGIN      
        1 aataatagct caatcccgcc tcacgccaaa atgcggccac cccggaccat gcatgaaatc
       61 gccgcgataa ttgagcagga tcaagcaagg gcgcgagcgt gttcatgaat agtacggccc
      121 ggcaccagca cccccgctac aatccacata tggcccgcat cgtggaaatt catccggtga
      181 acccccagcc acatcggatt gcaagcgtcg ttggcgcgat ccgcgacggc ggcctcatcg
      241 cctatcccac cgattcgagc tacgcgctgg gatgtcagat aggcgacaag aaagccatgg
      301 accggatcca ccggatacga cggaccgaca agaaccataa cttcaccctg gtggtacggg
      361 acctgtcgga aatcagcgta tatgcacggg tcgacaactg gatttatcgc ctgctgaagt
      421 cgctcactcc ggggccgtat acggtgatct tacaggccac ccgcgaggta ccaaagcgtt
      481 tgcagaatcc caggcggcgc acgattggcg tgcgtgtgcc ggatcatccg atagtcgccg
      541 cgcttctcga cgcgctgggg gaacccatca tgagttccac cttgcattta ccgggcgatg
      601 accttccact gaccgatcca cacgagatca acagtcgcat cggacacgaa atcgaggttg
      661 tcgtagatgc gggtcccaca ggaatagagc cgaccacggt cattgatctt tcggggggag
      721 ccgtggaaat cctgcgccgg ggccgcggag atgtcagttt tcgcgcctga tgcagcagtt
      781 gccgtggctc gagcaaatca ggttaaaatg ccggcagccc atgaaaccag aattagtagt
      841 gctgacaagc aaagacgcgc cgaagcccgc gtcgaagggc gacggcggtc cattccaggg
      901 cgaaatgccc tttgcggtag tagacggcga gccggtcacc cagctgcccc aggacctgta
      961 cataccccct tacgcgctgc aggtcttcct cgaagccttc gaaggaccgc tggatctcct
     1021 tctgtacctg attcgccgcc agaacctcga tattctcgat ataccgatcg ccgagattac
     1081 acggcaatac gtccagtaca tcgagttgat gaaagaactg cacctggaac ttcccggcga
     1141 atacctgctg atggcggcga tgctcgcgga aatcaagtcg cgcatgctgt tgccgcggcc
     1201 gcaggcggag gaagaggaag aagatgatcc tcgcgctgag ctggtgcgcc gcctgcagga
     1261 atacgagcga ttcaagaaag ccgcggaaga actgggggca ttgccccggc tcgaacgtga
     1321 cgtgttcgtc gcctccgtgg aggcacctga gcggaaagtg gtggcgcagc tgcccgatgt
     1381 cacgctgaaa gagttgctga ttgcgtttca cgacgtgctg aagcgcgccg aaatgttttc
     1441 gaggttgcac ctgcggcgag agccgttgtc ggtgcggcag cgaatgtccg aaattttgct
     1501 gcgggtaaag gccaacacgt tctccagctt ttccgatctt ttcgatcccg aggaggggcg
     1561 catgggcgtt gccgttacct tcatcgccat tcttgaactg ctgcgggagg ccgccatcga
     1621 agtcgcacag gccgaggcgt tcgcaccgtt gcacgtgcgc gccgcctcct cggtgcggct
     1681 cgtttcagat gacggcgtcg cctgatcgtc ctgcagtttc ccgccaacat agtagagacg
     1741 catcgtggac cagaacgaga tcaagtactt cgtggaagca gccctgctgg cggccggcag
     1801 gccgctttcc atcgatcagc tgcagaacct gttcgaccgc aattctgtgc cggaaaggac
     1861 gcagatcagg gaagcgatca gcggcctcca ggaagattat gaagagcgcg gcctggcgat
     1921 atcggaggtg gcatccggct tccgtacaca aatcaaggct tcgatggcag accgcctgca
     1981 gaagctgtgg gaagagcggc ctccccgtta ctccagggct tttttcgaga cacttgcact
     2041 tatcgcatac cggcagccta tgacgcgggg cgaaatcgag gaaatccgcg gggtggcagt
     2101 gagcgccaac atcatgcgct cgctcctgga gcgggactgg atacgggtgg tgggtcatcg
     2161 cgacgtgccc ggccggcccg cgatgtttgg caccacgaga cacttcctcg actacttcgg
     2221 attgaagaag ctcgacgacc tcccgccgct tgccgacctt gcggactggg agacgcttcg
     2281 tgtccaactt aatcttcccg aggttagcga tgaagaggcg gagccggaaa ccgccgagat
     2341 agccgacctg cccgtcctcc tgtaccccca aagctaccag gcggacgacg aggcgggcgc
     2401 cgcgagtcct ggcgaggacg aggcgtccgc agaagatatc gcggacgcag cagcgaccgc
     2461 cggcttcgag gctatcccgc caaccaggtg gccgggtccg gttgaaatcc tgcgcgtcgt
     2521 gcccggtgac gatattgaag acccggcgga agatgaggcc gggaaggttc ttaaggacag
     2581 ggacgcattg atctccagac gttagtccgg gccagggaaa gccgcgtgtc cgaacgtatc
     2641 cagaaagtcc tcgccgcggc cggccacggt tcgcgtcgcc agatcgaagg ctggattcgc
     2701 gagagccggc tgcgcgtcga cggccgtgtc gccacgctcg gcgaatccgt ctcgggcagc
     2761 gaaaccatca cactcgacgg ccgcagcctc gcagttcgtc ccgcagcagg agcacaccgc
     2821 cacatcatct acaacaagcc cggcgacgaa ataacgtcac gctccgatcc ggaggggcgc
     2881 cgggtcgtgt tcgattccct tccaaagttg agcggctcgc gctgggtagc agtcggccgg
     2941 ttggatatgg caactaccgg actgctgatt ttcaccaccg atggcgatct ggcaaatgcc
     3001 ctcatgcacc cctcatcgga gctcgttcgc cgttacgccg tcagggtgca tggcgctccc
     3061 agccaggccg acgtcacccg cttgctcgaa ggtgttgagt tggaggaggg cggtcgcgcc
     3121 gcgttcgaca gcatcgaacg ttcaggaggt gacggtgcca atcgatggtt cacggtcacc
     3181 ttgcgcgaag gccgcaacag ggaagtgcgc aagatgtggg ctgcggtcgg ttacgaggtc
     3241 agccggctga tacgcactgc ttacggaccg ctcgaacttc ctcgcagcct gcgtcggggg
     3301 aagtacgtgg cactgacgcc ggcgcaggtg cgcacgcttt attgttctgc gggactggac
     3361 ccgccgagac cagccagtat gtcgcccgca tcccgcggcc gtcggccgcg acatgcgcgc
     3421 gcaaagtcta gataaaacaa atcgttataa agtaataatg atgtataacc tcactgcgag
     3481 tcgaggctca tcccggttac ttcccggctc tctggcccca gcaggaacag gtaaggggag
     3541 aggacctcag tgggcggctt cgatgcactc ttctcttcgg ccgggtatgc ctgcagtctt
     3601 aattcggtgc gcgtggggcc gggattgacg caattcaccc ggatcgggac gtgcttgttc
     3661 tccgcggcga gaatttgcgt cagggcctct ataccgaact tggagacgga atacgctccc
     3721 cagtaggggc gt
//
LOCUS       DASUOM010000581         6413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1527317,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000581 DASUOM010000000
VERSION     DASUOM010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527317"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1915
                     /locus_tag="VFY27_12115"
                     /pseudo
     CDS             1..1915
                     /locus_tag="VFY27_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            2029..3342
                     /gene="odhB"
                     /locus_tag="VFY27_12120"
     CDS             2029..3342
                     /gene="odhB"
                     /locus_tag="VFY27_12120"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5766308.1"
                     /translation="MSIEIKVPALPESVSDATLVAWHKKPGDPISRDENLVDLETDKV
                     VLEVPAPTSGTLKEIRIEDGATVNAGDVLAILEEGEGAGAVSGNGADEEQETTAAPEP
                     APAARQPEAAAKQDEEDGEDEAADERGVESRGESAHKLSPAVRRLLDEHDLEATIVSG
                     SGRDGRITKSDVMEYLKSHSDENVAPGDPEPRPDGAEAREARGSSRSQQRVPMTRLRA
                     KIAERMVQAQSTAAMLTTFNEIDLTQVISMRNRYKEAFQKQHDVKLGFMSFFVKASVE
                     ALRKFPVVNASIEGNDIVYHDYFDIGIAVSSERGLMVPVLRDVDQLSFAQIESDIERL
                     GKKARDGTMTMEDLTGGTFTITNGGIFGSLLSTPILNPPQSAILGMHAIQERPVVVDG
                     EIAVRSMMYVALTYDHRIIDGREAVQFLVSIKSSLEDPARMLLEF"
     gene            3395..4822
                     /gene="lpdA"
                     /locus_tag="VFY27_12125"
     CDS             3395..4822
                     /gene="lpdA"
                     /locus_tag="VFY27_12125"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5766309.1"
                     /translation="MARTFDVIVIGAGPAGYIAALRASQHGMNVACIDEWENRDGKSA
                     FGGTCLNAGCIPSKALLESSELVHRAEHEFSKHGIKVSGLEVDLASMQKRKQGIVRQL
                     TGGIAGLFKANKVEGLVGHGKLLADRKVELTPKSGEPEILEARYVILATGSAPVELKI
                     APFDGDRIVDSWDALEFEKVPRTLGVVGAGVIGLELGSVWSRLGSEVVVLEAMDEFLA
                     AADRDIASEAAKQFKRQGLAIHLGAKVAAAAASETGVRVDYEGSSGKQSIEVEKLVVA
                     VGRKPYTNGLLGKDCGIQLDERGFIPVDQECRTRVKGVFAIGDCVRGPMLAHKGSEEG
                     VMAADLIAGGVAEMNYDVIPSVIYTAPEIAWVGKTEQEVKASGREYKTGSFPFAASGR
                     AKGMEQTAGMVKIISALDDDEILGVHILGPMAGELISEAVLAMEFSASTEDIQRTIHA
                     HPTLAEALHEAALAVDNRALNNINK"
     gene            complement(4860..>6413)
                     /locus_tag="VFY27_12130"
     CDS             complement(4860..>6413)
                     /locus_tag="VFY27_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5766310.1"
                     /translation="RKEGGSDLILLALEDVTERNLSEKAKADLAAIVESSDDAIVSKD
                     LDGVIRSWNRGAERIFGYSSEEIIGQSIMRIVPPDLQHEEREILASLRRGNGVDHYET
                     VRLTKDGQRLDVSLTISPVNDSSGKVIGASKIARDISDVKRTAQALKDANRRKDEFLA
                     MLAHELRNPLSAISSAVELLGTPDGGNHLDVARLIIGRQTVHLARLVDDLLDMSRITR
                     GVVALRKDVLNVNTLIDGAVEQVKPLIEQRRHVLEVSVPRKPLWLEGDATRLEQLFAN
                     LLTNSAKYTEPGGRIEVKARRLIDTILITVRDNGIGISAKLLPDVFELFSQSDRSADR
                     SAGGLGIGLTLVKSIAEMHGGNVEAKSGGLRKGSEFTVRLPALAVDDKRKKAQARSVP
                     VANSGPARRILVVDDNEDAALALSRLLEASGHIVFQAYDGPSSLEAAKEHEPDVVLLD
                     IGLPGLDGYEVAKRMRQDPALSEVLLIALSGYCQEEDRQRSREAGFDHHLAKPTDHKQ
                     LLELLAAAR"
BASE COUNT         1334 a         1902 c         2030 g         1147 t
ORIGIN      
        1 gtaccacaag ggcttctccg cggatatgaa aacgccgggc ggcaacgtgc acattgcgct
       61 ggcgttcaat ccgtcgcacc tggagatcgt cgaccccgtg gtcgagggca gcgttcgcgc
      121 gcggcagcaa cgcaggggcg atttcgagcg tgcggaagtg ctgccggtac tcatccatgg
      181 cgacgcagca tttgcggcgc agggggtggt cgcggaaacc ttccagctgt ctcagacgaa
      241 cggtttcagg accggcggca ccgttcacat cgtgatcaat aaccagatcg gctttaccac
      301 cagccggccg tcggacgcgc gttccacgcc gtactgcacc gacatcgcga aaatgatcga
      361 ggcgcctatt tttcacgtaa acggggacga tcccgaggca gcgattttcg tcacccgtct
      421 cgcgctcgag taccggcaac gctacaagaa ggacgtcgtc atcgacctcg tgtgctaccg
      481 gcgtcacggg cacaacgagg cggacgagcc gagcgccacc caaccggtca tgtacggcac
      541 gatccgcaag cacaggacga cgcgcgagct ttacgccgat cgcctggtcg aaggcgggct
      601 gatcgacaag gaaggcgtcg ctgcactgca ggaaaactat cgcgaccggc tggatcgcgg
      661 ggaaccggtg ccgaaatcgt ccctcgggct gatcggcaac gagtacacgg tcgactggaa
      721 ccggtacctg gacgcgagct gggacgagga agtggatacc acgctcgagg tcgcgaaggt
      781 cgccgaactg tcgaagaaga ttaccgcgct gccgccgggc ttcgtactgc acgggcgcgt
      841 gaagaggatc atcgacgaac gtgtccgcat gggtgccggc gagatcgata tggactgggg
      901 tttcgctgaa accatggcct acgccggcct gatcgacgac ggctacgact gtcgtgtcac
      961 cgggcaggac acctcccgcg gcaccttctt tcaccgccac gcggtattgc acaaccagcg
     1021 caaccgggag gagttcatcc cgttacagac gatctccgat cggccttcgg cgtttcgcgt
     1081 catcgactcg ctcctgtcgg aggccgcggt gatgggcttc gaatacggct acgcgacgac
     1141 cgagccaagg acgctggtag tgtgggaagc ccagttcggg gacttcgcca atgctgcaca
     1201 ggtcgtgatc gaccagttca tcagctccgg cgaggcaaaa tggggccggc tttgtggact
     1261 taccctgttc cttccccacg gctatgaagg ccagggtccc gagcactcct cggcgcgact
     1321 ggaacgtttt ctgcaacttt gcgcggagaa caacatgcag gtctgcatac cctccactcc
     1381 ggcccagatg tttcacatgc ttcggcgcca gatgctgagg ccgtatcgca agccgcttgt
     1441 cgtcatgacg ccgaagagcc tgctgcgcca caaactgtcg gtgtcgccgc tgtcggatct
     1501 ctcggaagga cagttccacc tggttattcc ggaagtcgag gagatcgacg gggaagccgt
     1561 caagcgcctc gttttctgca gcggcaaggt ttactacgac ctgctgcagg cacggcgttc
     1621 gcaggaattg tccgacgtgg ccttgatccg gatcgagcag ctttatcctt tcccgattga
     1681 ccggtatgag gcgcagatcc agtcgtaccc gaatgccgaa gccgtcgtgt ggtgccagga
     1741 ggaaccccag aaccagggag cgtggtacca gattcgtcac cggctgcagg aacccctgaa
     1801 cgaccgccag cagctctact acgcgggccg ggagagcgcg gcggctcccg cctccggcgt
     1861 gctgaaactg catatccagc agcagcagga gctggtcgag gcggcgctgg gcatcgagat
     1921 cgccgccagc gaatccgacg gaacgatcga caagccaaag aagaaaaaaa ccggtcgcaa
     1981 aggcaagcca gggagcaaaa cgcgaaaaag caatgaacgg actaacgcat gagtattgag
     2041 ataaaagtcc cggcactgcc ggaatcggtc agcgacgcga cgctggtggc ctggcacaag
     2101 aaaccgggcg accccatctc ccgagatgaa aacctggtag acctcgagac ggacaaggtg
     2161 gtcctggagg ttcctgcccc gacttccggc accctgaagg aaatcaggat agaggacgga
     2221 gcgacggtca atgccggcga tgttctcgcg atcctggaag aaggggaggg tgccggggcg
     2281 gtttccggca acggcgccga tgaggagcaa gagaccacgg ctgcccccga gccggcccct
     2341 gcagcgcggc aacccgaagc agcggcaaag caggatgaag aagacggaga ggacgaagct
     2401 gcagacgaga ggggcgttga atcccgcggt gaaagcgctc acaagttgag ccccgcggta
     2461 cggcgcctgc tcgatgagca cgacctggaa gcaacgatcg tatcgggaag cggtcgcgac
     2521 ggccgcatca cgaaatccga tgtcatggag tacctgaaga gccacagtga cgagaacgtg
     2581 gcgccgggag atccggaacc gcggccggac ggcgcggaag cccgtgaagc gcgtggaagc
     2641 tcgcgatcgc aacagcgcgt gccgatgacc aggctgcgcg caaagatcgc cgagagaatg
     2701 gtgcaggcgc agagcaccgc ggccatgctc accacgttca acgagatcga tctcacacag
     2761 gtcatctcga tgcgcaatcg ctacaaggaa gccttccaga agcagcatga cgtcaagctc
     2821 ggcttcatgt cgttcttcgt caaggcatcg gtcgaggcgc tcaggaaatt cccggtcgtg
     2881 aatgcctcga tcgagggcaa cgacatcgtc taccacgatt atttcgacat cggcattgcg
     2941 gtgtcgtcgg agcgcggatt gatggtgccg gtcctgaggg acgtggacca gctgagcttc
     3001 gcgcagatcg agagcgacat cgaacggctt ggtaaaaagg cgcgcgacgg cactatgacg
     3061 atggaggacc ttaccggcgg cacctttacg attacgaacg ggggtatctt cggctcgctg
     3121 ttgtcgacgc cgatcttgaa tccgccacaa agcgccatcc tcgggatgca cgcgatccag
     3181 gagcgcccgg tcgtcgtcga tggcgagatc gcagtacgtt cgatgatgta cgtcgcgctg
     3241 acgtacgacc atcgcatcat cgacggtcgc gaagcggtgc agttcctggt cagcatcaag
     3301 agctcgctgg aagacccggc caggatgctg ctcgagttct gagccggcgc atatcctcac
     3361 cgacgaagga aagcaatgga agtgagagaa gcggatggct aggactttcg acgtcatcgt
     3421 gattggcgcg ggccctgcgg gttacattgc ggcactccgg gcgagccagc acggcatgaa
     3481 cgtggcctgc atcgacgagt gggagaatcg cgacgggaag agcgcgtttg gcggcacctg
     3541 cctcaacgca ggctgcatcc cttcaaaggc actgctcgag tcttccgagc tggtgcatcg
     3601 cgcagagcac gagttcagca agcacggcat caaggtgtca gggctggaag tggacctggc
     3661 atcgatgcag aagcgaaagc aaggcatcgt gcgccagctt accggcggga tcgccggact
     3721 tttcaaggcg aacaaggtcg aaggactggt tggacacggc aagcttctcg ccgaccgcaa
     3781 agtcgagttg accccgaaat cgggcgaacc ggaaatactg gaagcgcgct acgttatcct
     3841 cgcaacgggt tcggcgccgg tcgagctgaa gatcgcgcca ttcgacgggg atcgcatcgt
     3901 tgattcctgg gacgcactcg agttcgaaaa ggtgccgcga acactcgggg tcgtcggggc
     3961 aggcgtgatc gggctcgagc tcggcagtgt ctggagccgc ctcgggtcgg aggtggtcgt
     4021 tctcgaagca atggatgaat tcctcgcggc ggcggaccgc gacatcgcga gcgaagcggc
     4081 gaagcagttc aaacgccagg gcctggcgat tcaccttggc gcgaaagtgg ccgctgccgc
     4141 ggcctcggaa acgggcgtca gggtcgacta cgaaggttcc tccggaaagc agtccatcga
     4201 agtcgagaag ctggtggtcg ccgtcggccg taagccatac accaacggcc tgctcggcaa
     4261 ggattgcggc atccagctcg acgagcgcgg cttcattccc gtcgaccagg agtgccgcac
     4321 gcgtgtcaaa ggcgtgttcg cgatcggcga ttgcgtgcgt ggcccgatgc tcgcgcacaa
     4381 gggatcggaa gaaggcgtga tggcagcgga cctgatcgca ggcggggtcg ccgagatgaa
     4441 ctacgacgtg attccttcag tcatctatac cgcgccggag atcgcctggg tcgggaagac
     4501 ggaacaggaa gtcaaagcca gcgggcgtga atacaagacc ggctccttcc cgttcgctgc
     4561 gagcggccgc gccaagggca tggaacagac cgccggtatg gtcaagatca tctccgccct
     4621 ggacgacgac gagattctcg gcgtgcacat actgggcccc atggccggcg aattgatctc
     4681 agaggccgta ctggccatgg agttttcggc gagtaccgag gatatccagc gcactatcca
     4741 cgcgcacccg acccttgcgg aagccttgca cgaggcggca ctcgcggtcg acaaccgcgc
     4801 cctcaacaac atcaacaagt gatcaggtta acgagtacat ctgtcccctt tatgaacgct
     4861 taacgagctg ccgcgagcag ctcgagcaac tgcttatggt cggttggctt ggcgaggtgg
     4921 tgatcgaaac ctgcttcccg cgagcgctgg cgatcttctt cctggcaata tcccgaaagc
     4981 gctatcaaga gcacttccga cagcgcgggg tcctgccgca ttcgtttcgc tacttcgtaa
     5041 ccgtcgaggc ccggcaggcc gatgtcaagc aggacaacat cgggctcgtg ttccttagcg
     5101 gcctcgagcg aggatggccc gtcgtaagcc tgaaacacaa tatggccgga agcttcgagc
     5161 agccgggaga gcgccagtgc cgcgtcttcg ttatcgtcca cgaccaggat gcggcgggcg
     5221 ggaccggaat tcgcaactgg tacggaccgg gcctgggctt tcttgcgctt gtcgtcaacc
     5281 gcgagtgccg gtaagcgtac cgtgaattcg ctgcccttgc gcagccctcc acttttagct
     5341 tcgacgtttc cgccgtgcat ctccgcgatt gacttgacca gcgtcaggcc gattccgagc
     5401 cccccggcgg agcgatcggc ggaacgatct gactgcgaga agagctcgaa cacgtccggc
     5461 agaagtttgg cggaaatgcc aatgccgtta tcgcgcactg taatcaggat tgtgtcgata
     5521 agtcgtcgcg cttttacttc gatgcggcct cccggttccg tgtacttggc cgagttggtt
     5581 aacaggttcg cgaacagttg ttccagccgg gtcgcatcgc cctccagcca cagaggcttc
     5641 cttggcaccg agacctccag gacatgacgc cgttgttcga tgaggggctt tacctgttcg
     5701 acagcgccgt caatcaaggt gtttacattc aaaacatcct tgcgcagcgc cacgactccc
     5761 cgcgtgattc gtgacatgtc cagcaggtca tccaccagcc gcgcgagatg cacagtctgg
     5821 cgtccgatga tcaggcgagc cacgtccaga tgattcccgc cgtcgggcgt accgagaagc
     5881 tcgacggcgc tgctgatggc agacagcggg tttctcagct catgagcgag catggccagg
     5941 aactcatcct tgcggcggtt ggcatccttc agcgcttgag cggttcgttt cacatcgctg
     6001 atgtcgcggg cgatcttcga cgcgccgatg accttgccgg agctgtcgtt caccggagaa
     6061 atggtcagag atacgtcgag tcgttgacca tctttggtaa gccgcacggt ttcgtagtgg
     6121 tcgacgccat tgccccggcg cagacttgca agaatttccc tctcttcgtg ctgcaggtcc
     6181 ggaggaacga tccgcattat cgactgaccg attatttcct ccgagctgta gccgaaaatg
     6241 cgttccgcgc cgcggttcca gctccggatg acgccgtcga ggtctttgct cactattgcg
     6301 tcgtcggagg attcgacaat ggcggcgaga tcggcctttg ccttctcgga caaattgcgt
     6361 tcagtcacat cttccaatgc gagcagaatc aagtccgagc cgccttcttt ccg
//
LOCUS       DASUOM010000582         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1408711,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000582 DASUOM010000000
VERSION     DASUOM010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408711"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFY27_12135"
     CDS             complement(<1..295)
                     /locus_tag="VFY27_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766311.1"
                     /translation="MNSKFLIRNAVRRALCVGALATVAGYLPTAAAQEPEDEALEEIT
                     VTGTRIMRQDYQSASPISTVDADLFQQTGAPTIETTLNTLPQFVPAITTTSNNP"
     gene            complement(699..2231)
                     /locus_tag="VFY27_12140"
     CDS             complement(699..2231)
                     /locus_tag="VFY27_12140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5766312.1"
                     /translation="MKAHFLPLCALTGDDHAGACTNARVEANAGEYPAMRMRSQSAPL
                     KPSVLVSFALMALMLAEIPLASPRFERPAGVADANEPLIAAGYRALFTCSAYFFSGRK
                     LADIERVELVDTAPLRLPAPEIDETRQLVAAADGAGNVQVAAYRDSMGCTLLPPHWDE
                     ADLPRLPYVAYPDAPDVRDVPFPRGDATDLGRDGLQRSARGLRNVIEQAFNGTSYGKD
                     VVTVGIVVVRDGEIIAERYRPGFGVHKGYRTWSTAKSITAALLGIAARKGLLDLDAPA
                     AIPEWQYPGDPRRAITYKHLLWMSSGLSSGGNNTYAVYFGGQDVVSAVTTTPLEVEPG
                     SRWKYANNDTLLLLRALRHVLGSDLQYLRFPYDELLHRIGMFHTRMETDHLGNFIGSS
                     QVYTTARDLARFGVLLANDGVWEGERLLPEDWVRFSRMPAPAKPVEPGTRGYGAQFWL
                     LGSIAGIPEDTYTTAGNKGQYVTVLPSGLVIVRTGVDPLGTSWEHERFVADVISELNQ
                     AD"
     gene            2220..>2693
                     /locus_tag="VFY27_12145"
     CDS             2220..>2693
                     /locus_tag="VFY27_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766313.1"
                     /translation="MSFHLSSSLDRDVVFDSFSQQGYAHIRNVLPAENAKRIHKGLTE
                     QTPWNLAFNNKGKHIDLPAAQLAVMPKQSVNQLQQAIYAQARDGFQYCYTNYPIYDAY
                     QAGLNRGHVLHQFYEWMNDEEFLQFARAATGFDDISFVDAQATRYMPGHFLTTHDD"
BASE COUNT          545 a          828 c          820 g          500 t
ORIGIN      
        1 atgggttgtt cgatgttgtc gtgatcgcgg gcacgaactg cggcagcgtg ttgagcgtcg
       61 tttcaattgt cggcgcgccc gtctgctgga acaggtctgc atccacggtc gagatcgggc
      121 tcgcggactg gtagtcctgg cgcatgattc gcgtccccgt gacagtaatc tcctcgagcg
      181 cctcgtcctc gggttcctgg gcagcggcgg tcgggagata gcccgcaacg gtcgcaagcg
      241 caccgacgca caaggcgcgc cgcactgcat tccgtatcaa aaatttactg ttcatcattg
      301 cctcctgaag cgaagtcacg cggctcatcg ccacggaaaa actgtcggaa ccagaccggg
      361 gaacggtcga aatttcgtcg ttgcaccggc gggtcaggac gacccgaact gtagagacct
      421 ggaacggcct cgggtgcgaa tagcctacgt cgtgttgctg tgaacttaca accggtcaac
      481 cggcgtgcga catcgctccg ccagccgtca aagcccggaa aatcaaatcc gatccgcgcc
      541 tggttcgaga aatttcagga gtttaaaacc cgcgccgagg caagcgttgc aaaaacgaaa
      601 caacgggaag ggtgctgctt atgacaaatc gttctatccc gaaggcgggt gcaagaggag
      661 ttgcggatgt aaacccgcta cgggaagaag aacgtcgatc aatctgcctg gttcagttcc
      721 gagataacgt ccgctacgaa gcgctcgtgt tcccaggaag tgccgagcgg atcgacgccc
      781 gtacggacga tgaccagccc ggacggcaag accgtgacgt actggccctt gttgccggcg
      841 gtcgtgtacg tgtcttcggg gatgcccgct atcgaaccca gcagccagaa ctgcgcgcca
      901 tagccacgcg ttcccggctc gaccggtttt gcaggtgcgg gcatccgtga gaaccggacc
      961 cagtcctccg gaagcaggcg ttctccctcc cagaccccgt cgttggcgag cagcaccccg
     1021 aatcgtgcca ggtcccgggc ggtggtgtag acctggctcg atccaatgaa gttgcccagg
     1081 tggtcggttt ccatgcgcgt gtgaaacatg ccgatgcgat gcagcaactc gtcatacggg
     1141 aacctgaggt actgaaggtc gctcccgagc acgtggcgca gcgcacgaag taacaacagg
     1201 gtatcgttgt tcgcatactt ccagcggctg ccgggttcga cctcgagcgg cgtggtcgtc
     1261 accgcgctca cgacgtcctg gccgccgaag taaacggcgt aagtattgtt accgccgctc
     1321 gagaggccgc tcgacatcca cagcaggtgc ttgtaggtga tggctcggcg cgggtcgccc
     1381 ggatactgcc actcgggaat cgcggccggt gcatccaggt cgagcagccc tttgcgcgcc
     1441 gcgataccaa gcagcgctgc cgtgatgctt ttcgcggtcg accatgtgcg gtatcccttg
     1501 tgtacgccaa agcccggacg atagcgttcg gcgatgatct cgccgtcgcg tacaacgact
     1561 atccccaccg ttaccacgtc cttgccgtag ctggtcccgt tgaaagcctg ttcgatgacg
     1621 ttcctcaagc cgcgcgcgct ccgttgcagg ccgtcgcggc cgaggtccgt ggcgtcgcct
     1681 cgaggaaatg gaacgtctcg tacgtcaggt gcgtccgggt aggccacgta tggcagccgc
     1741 ggaagatccg cctcatccca gtgtggcggg aggagcgtgc accccatgga gtcgcgatat
     1801 gcagcgacct gcacattgcc cgcgccgtcc gcggcagcta ccagctgcct tgtctcgtca
     1861 atctccggcg ccggcaaacg gagcggcgcc gtatctacga gctcgacgcg ttcaatgtcc
     1921 gcaagcttcc tgccggaaaa aaaataggcg gaacaggtaa agagcgcgcg gtaacctgcc
     1981 gcgatcagtg gttcgttcgc atctgcgacg cctgccggcc gctcgaacct gggcgaagcc
     2041 aaaggtatct ccgccagcat gagcgccatc agtgcaaagg acaccagcac tgatggcttc
     2101 agcggtgcgg attgactgcg catccgcatg gccggatatt cacccgcgtt ggcctccacg
     2161 cgggcgttgg tacatgcgcc tgcgtggtca tcgccggtga gcgcacaaag aggtaggaaa
     2221 tgagctttca tctgagttcg tccctggatc gcgacgtcgt tttcgacagt ttctcgcagc
     2281 agggatatgc ccacatccgg aatgtgctgc cggcggaaaa cgcaaagcgg attcacaaag
     2341 gcctgacgga acaaactccc tggaacctcg ccttcaacaa caagggcaag catattgacc
     2401 tgcccgccgc ccagctggcg gtcatgccga agcaaagcgt aaaccagttg cagcaggcaa
     2461 tctatgcgca ggcgcgcgac gggttccagt attgttacac gaactacccc atctacgatg
     2521 cctaccaggc cggactgaac cgcggccacg tgctgcacca gttttacgaa tggatgaacg
     2581 acgaggagtt cctgcagttc gcgcgagcgg ccaccgggtt cgacgacatc agtttcgtcg
     2641 atgcacaggc cacgcggtac atgccgggac acttcctgac gactcacgac gac
//
LOCUS       DASUOM010000583         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1281792,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000583 DASUOM010000000
VERSION     DASUOM010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281792"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFY27_12150"
     CDS             complement(<1..682)
                     /locus_tag="VFY27_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5766314.1"
                     /translation="MAQLGFGLIGTGFMGRTHAIALQSVATVFAGIEAPRLVTVADVN
                     ADSAATAGRDLRFERSTVDWKELLHDPAIDVIDICAPNYLHYPMSLAALHAGKHVYCE
                     KPLALGVDEAAELAALAGRSGLVHAIGLNYTTNPLVQTAREIVSSGEIGEPISFTGRY
                     FEDYMADPAVPHSWRCERRFAGAGALADLGSHLIDMAQTLLGPIDSVFASVETVHAKR
                     RVPATGEMR"
     gene            complement(794..1786)
                     /gene="iolG"
                     /locus_tag="VFY27_12155"
     CDS             complement(794..1786)
                     /gene="iolG"
                     /locus_tag="VFY27_12155"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX5766315.1"
                     /translation="MTINLCLFGAGRIGRIHGANIAAHRDATLKYVVDVDPAAAARLA
                     AGLNSAAADVQAALSDDDVQGVVIATSTDTHLSLIEAAASAGKAVFCEKPLDLDETRA
                     RRCIEVAAAAAIPLYVGFNRRYDPSFRRLKDEIAAGTIGAVEVVSITSRDPSPPPADY
                     IRRSGGMFRDMTIHDLDMARWLLGEEPSSVFASGSCLVDPAIREAGDIDTAVVILETR
                     GGAFCQVTNSRRCSYGYDQRIEVFGARGMLRADNETETRVELASSAGFQREPALPFFL
                     ERYREAYRLEMDDFIRALKGEETSLADGNDGLRALILADAAERSRQSGKAERPA"
     gene            complement(1783..2646)
                     /locus_tag="VFY27_12160"
     CDS             complement(1783..2646)
                     /locus_tag="VFY27_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX5766316.1"
                     /translation="MKPRKQPAREPADLEQLRAAIVARHDELSPRLRQVAAYVLDHPN
                     DMGLQTLAVIAERCGVQASTIVRFAKAFGYAGASQMQRLFRDELISSSPSSSYAERIR
                     QFNERTGDGRELSPHELMQEFAASNIIALEHIKDTISASDLERSVDLMEQAAAVYLVG
                     LRRSFPVAAYMAYALRHVAKPVHLIDGLAGMLVEQSSMITGNDLLFAISFRPYAKETG
                     EIVRKAKGAGARVIAISDSELSPVSRDADISFDIRDAEVRQFRSLTGSLCVAQTLVIS
                     YAHRMEGQDNL"
     gene            2929..>4378
                     /locus_tag="VFY27_12165"
     CDS             2929..>4378
                     /locus_tag="VFY27_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5766317.1"
                     /translation="MPARLSFACRTVFALSLMFLASLANAQVQLRGQVTDAATARPLP
                     SAEVRIVELDRREATSNSGDFAIANIPPGDYTVQVRFVGYAPAEQSVQVSATGAQTLD
                     FELTAIGTTEEIVVTGYRAAQANALQDKQMSSVIKETLTADDAGKLPDQNVAEALRRI
                     TGVTATVDQGEGRYVTVRGVDPSYTNITLDNQVIGSPEDTRRVALDTIPSEVLSRVEV
                     VKAVTPDMDGHAVGGSINVITPSAFDDPDGSFFSATADYGYYDMNGDNPFGISAGWGQ
                     VFGPDEKWGILLSASYSERNYDTENLQGGDPWEEEGDFLIPDSLVLRDYRIERLRSGL
                     VANLEYRPTDEVKLYFRNLINEFEDTENQAETVWAYREGDLENQTATSGTFTEGEGER
                     LVSERNEKQSIRTSTLGGEFILGNWVLDASATYGEAEQETPYDREWVFEWSEPGPMTY
                     DTSDLFFTVDAGPTFHDPDLYEFNEYARGGQNI"
BASE COUNT          804 a         1347 c         1387 g          840 t
ORIGIN      
        1 tgcgcatctc gccggtcgcg gggacgcggc gctttgcatg cacggtctcg acgctcgcga
       61 aaacggaatc gatcgggccg agcagcgtct gtgccatgtc gatcaggtgc gagccgaggt
      121 cggccagcgc gccggcgccc gcgaagcgcc gttcgcagcg ccagctgtgc ggcacggcgg
      181 ggtccgccat gtagtcctcg aaatagcggc cggtaaaact tatcggttca ccgatctcgc
      241 cggaggagac gatttcacgc gcggtctgta ccagtggatt cgtcgtgtaa ttgaggccga
      301 tggcgtgcac caggccgctc cttccggcca atgccgcgag ctccgcggcc tcgtcgacac
      361 cgagtgcaag cggcttttcg caatacacgt gtttcccggc gtggagcgcg gccagcgaca
      421 tcgggtagtg caggtaattc ggcgcgcaaa tgtcgatgac atcgatcgcc ggatcgtgaa
      481 gaagctcctt ccagtcgacg gtgctgcgtt cgaagcgcag gtcgcggccc gccgtagcgg
      541 cgctgtccgc attgacgtcg gcaacggtca cgagtcgcgg cgcttcgatg cccgcaaata
      601 ccgtcgccac cgactggagc gcgatggcgt gcgtgcggcc catgaatccg gtgccgatca
      661 gtccgaagcc tagctgtgcc atctcgcctt gaatccgttt gcttgtgccg ttgccgtgat
      721 ccggcctccc gtccgcagaa tacgcggttg aaagtaatgt ttcaatctgt caagaaatga
      781 aattactgtt ccatcacgcc ggccgttcgg ctttgccgct ctgtcgggaa cgttcagcgg
      841 cgtccgcgag gatcaaggcg cgcaggccgt cgttgccatc ggcaaggctg gtctcttcgc
      901 ccttcagcgc gcgaatgaaa tcgtccattt caagccggta cgcttcccgg tagcgctcga
      961 gaaagaacgg cagcgccggt tcgcgctgga agcccgcgct gctcgccagt tccacgcggg
     1021 tttcggtttc gttgtctgcg cggagcatgc ccctcgcgcc aaagacctcg atacgctggt
     1081 cgtagccgta gctgcagcgg cggctgttcg tgacctggca gaaggcgccg ccgcgggttt
     1141 cgaggatgac caccgcggtg tcgatgtcgc ccgcctcgcg gatggccgga tccacgaggc
     1201 aactccccga tgcgaatacg gaggagggtt cttcccccag cagccagcgc gccatgtcca
     1261 ggtcatgaat cgtcatgtcg cggaacatgc cgccggagcg gcggatgtag tctgccggcg
     1321 gcggggaggg gtcgcggctc gtgatgctca ctacctccac ggcgcctatt gtgccggcgg
     1381 cgatttcgtc cttcagccga cggaacgacg ggtcgtagcg gcgattgaag ccgacataaa
     1441 gcggaatcgc cgccgccgct gcaacttcga tgcaacggcg cgctctcgtt tcatcgagat
     1501 ccagcggttt ttcacagaat accgccttgc ccgcggacgc cgccgcctcg atcagcgaca
     1561 ggtgcgtatc cgtcgaggtg gcaatgacca ctccctgcac gtcgtcatcg gaaagcgccg
     1621 cctgcacgtc ggccgccgcc gagttgagac cggcggcgag tcgcgccgcc gctgctggat
     1681 cgacgtcaac cacgtacttg agggtggcat cacggtgtgc cgcaatgttc gccccatgta
     1741 tccggccgat gcgtccggcg ccgaacaggc aaaggttgat agtcataaat tgtcctgtcc
     1801 ttccatgcga tgcgcatagc tgataaccag ggtctgggcg acgcacaatg aaccggtgag
     1861 ggatcgaaat tgcctgactt ccgcatcgcg gatgtcgaag gagatgtcgg catcgcgcga
     1921 gacgggactc aattcgctgt cgctgatggc gatgacgcgc gcccccgcac ccttggcttt
     1981 gcggacgatc tcgccggtct ctttcgcgta agggcgaaag ctgattgcga acaggaggtc
     2041 gttgccggtg atcatcgagc tttgttcgac cagcatgccc gccaggccat cgatgaggtg
     2101 cacgggcttc gcgacgtggc gcagggcgta agccatgtaa gcggcgacgg gaaaagaacg
     2161 gcgcaggccg accaggtaca cggctgccgc ctgttccatc aggtcgacgg agcgttcgag
     2221 gtcgctggcg ctgatcgtgt cctttatgtg ctccagcgca atgatgttgc tggcggcaaa
     2281 ctcctgcatc agttcatgcg gggagagttc gcgcccgtcg ccggtacgct cgttgaactg
     2341 gcggatccgc tccgcgtagc tggagcttgg cgaggagctg atcagctcgt cacggaaaag
     2401 ccgctgcatc tggctggcgc cggcatagcc gaaagccttg gcgaagcgga cgatggtcga
     2461 agcctggact ccgcagcgtt ccgcgatgac ggcaagggtc tgcagcccca tgtcgttcgg
     2521 gtggtccagc acgtaggccg ccacctgcct gagccgcggg ctcaactcat cgtgccgcgc
     2581 cacgatggcg gcgcggagct gttccaggtc cgcgggctcc ctggccggtt gtttacgtgg
     2641 cttcatcgat ctggtccctt tgttcttggt tttattatga tttaagcata gtaacggcgg
     2701 cacggcaggt attccatcgc cggtaaatcc gtttcaccgg aactgcagag ttggaggcgg
     2761 cagcgcggga tatgaaacac caatttcata tttgcatgtt acaaaatttt tattctatac
     2821 tccatcgtaa cgtcgccgtc acgttacatc gccatcattg ccggtttctc gccgcaatgg
     2881 attcgtgaaa acgttgcggc ggcccggggt gcttttgagg ggatcatcat gcctgccaga
     2941 ctttcgtttg cgtgccgcac cgtttttgcc ttgagcctca tgtttttagc gtcgctggcc
     3001 aacgcacagg tccagttgcg cggccaggtt accgatgccg cgaccgcccg gcccctgccg
     3061 tcggccgaag tgcggatcgt cgaactcgac cgccgcgaag ccacctcgaa ttccggcgac
     3121 tttgcgatcg ccaacattcc cccgggcgac tacacggtgc aggtgcgctt cgtcggttac
     3181 gcgccggcgg aacagagcgt gcaggtgtcg gcgaccggcg cgcagacgct ggatttcgag
     3241 ctcaccgcga tcggtaccac cgaggaaatc gtcgttaccg gctatcgcgc cgcgcaggca
     3301 aacgcgctgc aggacaagca gatgtcgtcg gtcatcaagg aaacgctgac cgcggatgat
     3361 gccggcaaac tgccggacca gaacgtcgct gaagccttgc gccgcatcac cggcgtcacg
     3421 gctaccgttg accagggcga gggccgttac gtcaccgttc gcggcgtcga tcccagctat
     3481 accaacatca cgctggacaa ccaggtcatc ggatcgccgg aagacacccg ccgtgtcgcc
     3541 ctggatacga ttccttccga ggtgctgtcg cgcgtggaag tggtgaaggc cgtaacgccg
     3601 gacatggacg gtcatgccgt cggcggctcg atcaacgtca tcaccccgag cgcattcgac
     3661 gatcccgacg gcagtttctt ctccgcgacc gccgattacg gctattacga catgaacggc
     3721 gacaaccctt tcggcatttc ggccggctgg ggccaggtgt tcgggccgga tgagaagtgg
     3781 ggcatcctgt tgtccgcgag ttactcggag cgcaactacg acaccgagaa cctgcagggc
     3841 ggggacccgt gggaagaaga gggcgacttc ctgattccgg actcgctggt actgcgcgac
     3901 taccgcatcg aacggctgcg cagcggtctg gtagcgaacc tggaataccg gccaaccgac
     3961 gaggtgaagc tgtatttccg gaacctgatc aatgaattcg aggataccga gaaccaggct
     4021 gagaccgtct gggcctaccg cgagggggat ctggaaaacc agaccgcgac ctcgggtacg
     4081 ttcaccgaag gcgaaggaga gcgcctcgta tcagaacgca acgaaaagca atcgatccgc
     4141 acgtcgaccc tcggcggcga attcattctg ggcaactggg tgctcgacgc ttcagccacg
     4201 tatggcgagg ccgagcagga gacgccgtac gatcgcgagt gggtattcga gtggtccgaa
     4261 ccgggtccaa tgacttacga cacatcagac ctgttcttca cggttgacgc gggaccgacc
     4321 ttccatgacc ccgaccttta cgagttcaat gagtacgcac gcggtggcca gaacatcc
//
LOCUS       DASUOM010000584         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_440479,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000584 DASUOM010000000
VERSION     DASUOM010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440479"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VFY27_12170"
     CDS             <1..1530
                     /locus_tag="VFY27_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5766318.1"
                     /translation="QGDDVGTSVSTGDIDADGIQDLIIGSPRADLEAPISGSPGATYV
                     VFGRTAADGGFPPRTVLPLLAEGDGSDGFVLTGTDRSDSSGAAVGAGDINGDGVDDII
                     VGADLATATTADEGRTYVVYGRRASVGGFPSLIPLSSLPDGDGSTGFVLNGEERGDRS
                     GNRVTAADINGDGIEDVIVGAALANPGGTNRGRNDAGKTYVVYGRNDFPAEFELSSLA
                     AGDGSTGFVLYGAARNDAVNRNDYFGIALSGGDLNGDGIADLAIGATGADPGGREEAG
                     AAYVVFGRAAGSPTGPVLRIDGNASAVEGDYLQFKVTLSRPADENVSYQVRTIDGTAT
                     SPGDYGDKGGARLMAPGETEQIIWVPTVNDAAIEENETLRLRLSNPENAELGKASARG
                     TIVDNDDTSDWARLTVANARAAEGELARFELTLSKPTKQDVKFAVQTVDVTATANQDY
                     APKGGTRIIPAGEISATIFVEIAEDNLAEGVEKFRLRVEARSNNAVVVRGNATGTITV
                     P"
     gene            complement(1620..3113)
                     /locus_tag="VFY27_12175"
     CDS             complement(1620..3113)
                     /locus_tag="VFY27_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX5766319.1"
                     /translation="MRAALYARFSTDKQSDSSIEDQLRVCERLAATHGFTVIRRFSDA
                     AISGGTSNRPGYQAMLEAARRREFEVILAEDCSRLWRLLAEQAPRLAELADLDIHVVT
                     HDLDTRQESAAILGAVSGAMAEAYRREIARRTRRGLEGRARQKKPTGGKTFGFAGDEI
                     DPDQAPVVLEIFQRYAAGETQRAIAVDLNRRGIPSPGAAWKRRERATDGKWRVSAIHA
                     MLRNERYIGRIVWNRCRWIRSATDSSKRKRIENPRSEWIVRDGLAIIDLDLWARAQAR
                     RDTGYDSPRAYPRYLLSGLLECGLCGRKMIISGGRGHRYICGNRQTGVGCDNDLGVAR
                     TLAEDLILKPIARELLSREAVDRAVTAMRGMRVPPRPDPRLAELRRLVSEGVLSKDEA
                     APAMERLRPTPSIEPDLSQAADVYRQVVTHLRKSLDADDIAVARPLLRQLIGPITVVP
                     TTQDGERFLTANFPAVAANSARTAGEGRLVAGASFVCRFDPVTLRRT"
     gene            complement(3362..4228)
                     /locus_tag="VFY27_12180"
     CDS             complement(3362..4228)
                     /locus_tag="VFY27_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5766320.1"
                     /translation="MRRLNHQLKQLCKHNRDGSYGTQVQRERVLTLIANQLHELGFTQ
                     MHAKSLKPKHVEALTTRWLESGVAAGTMKNRMAALRWWADKVNKRNVVARSNDHYGLP
                     RRQYANGANRAKALEKTNLERVTDPHVRMSLELQAAFGLRREEAIKIRPRLADNGDWL
                     WLKPSWTKGGRAREVPIRTEAQREVLDRAKALAGAGSLIPAEKTYAEQLRLYEGQCIR
                     AGLSRMHGLRHAYAQQRYRELTGWDSPAAGGPASKSFSIHDRAQDQLARETIARELGH
                     DRPSISVVYLGR"
BASE COUNT          831 a         1447 c         1537 g          901 t
ORIGIN      
        1 cagggggatg acgtcggtac atcggtgagc accggcgata tagacgcgga tggcatccag
       61 gatctcatca tcggctcccc tcgcgccgac ttggaagcgc cgataagcgg gagtcccggt
      121 gcaacctatg tggtgttcgg ccgcacggcc gccgacggag ggttcccgcc tcggaccgtg
      181 cttccgctac ttgccgaggg cgatggcagc gacgggtttg tactcacggg aacggatcga
      241 agcgattctt ccggcgcagc ggtgggcgct ggcgacatca atggcgacgg ggttgatgac
      301 atcattgtcg gtgccgactt ggcgacagca accaccgcgg acgaagggcg aacttacgta
      361 gtctatggtc ggcgcgcttc cgttggcgga tttccctcgc tgatacccct atcgtcgtta
      421 ccggatggag atggcagcac gggttttgtc ctgaacggcg aagagcgagg cgatcgttcc
      481 ggcaatcggg tgactgccgc ggatatcaac ggggacggta tcgaggatgt aatcgtcggt
      541 gcggccttgg cgaacccggg cgggacgaac cggggccgca acgatgccgg aaagacatat
      601 gtggtctacg gccgcaatga tttccccgcc gagttcgagt tgtcttcgct cgcggcgggc
      661 gatggcagca ccggattcgt gttgtacggc gcggcccgaa acgatgccgt gaatcgaaac
      721 gactactttg gcatcgcgct cagcggcggt gacctcaatg gcgatggcat cgccgacctg
      781 gccatcgggg ccaccggagc cgatcccggt ggccgcgagg aggccggggc ggcgtacgta
      841 gttttcggcc gcgcggctgg atcgccaacg ggtcccgtgc tccgtatcga cggcaacgcc
      901 tccgcagtcg agggcgatta tctgcagttt aaggtaacgc tttcgcggcc cgccgatgag
      961 aatgtgagct atcaggtaag gaccatcgac ggtactgcca catcacccgg cgattacggc
     1021 gacaaaggcg gcgcaaggct catggcgccg ggcgagaccg agcagatcat ttgggtcccg
     1081 accgtcaatg atgcggctat cgaagagaac gagaccctgc gtctgagact gtcgaacccg
     1141 gagaatgccg aactgggcaa ggccagcgcc aggggcacca tcgtggacaa cgacgatacc
     1201 agtgactggg cgcgcctcac ggtggcgaac gcacgtgccg ccgaaggtga gttggcacgg
     1261 ttcgagctca cgctgtcaaa gccgaccaag caggacgtga agtttgcggt acagactgtc
     1321 gacgttaccg cgacggcgaa tcaggactac gccccaaagg gcggtacacg aatcattcca
     1381 gccggcgaaa tcagtgcaac gatcttcgtc gagatcgctg aggacaattt agcggaaggc
     1441 gtcgaaaagt tccgcctgcg cgtcgaggcc cgcagcaaca acgcggtcgt cgtgcgcggc
     1501 aatgcaacgg gcacgatcac ggtaccgtaa acttgccagt ggtgctagcg atcgttggct
     1561 gcgtaagcga cggcgctgat gcagtgcttg ctgatggtcg aagagactcg cagtatcggc
     1621 tatgttcttc ggagcgttac aggatcgaat ctgcatacaa agctcgcccc cgctaccaat
     1681 ctaccttccc cggccgttct cgctgaattt gcggcgacgg ccgggaagtt cgcggtcaga
     1741 aaacgttccc cgtcctgggt tgtcggaacg accgtgattg gcccgatcaa ttgccgaagc
     1801 agtggccggg ctaccgcaat atcatcggca tcgagcgact tccgcaagtg ggtaacgacc
     1861 tggcgataga catccgcggc ctgagacagg tccggttcta tcgacggggt cggtcgcagg
     1921 cgctccatgg ccggcgccgc ttcgtccttc gacagcacgc cttcgctgac caggcgccgg
     1981 agctcggcca ggcgcgggtc cggtctcggc ggtacccgca tacctcgcat cgctgtcacc
     2041 gcccggtcga cggcttcgcg ggatagcaac tccctggcga tcggcttcag gatcaggtcc
     2101 tcggccagcg tgcgtgcgac tccgaggtcg ttgtcgcaac caacgcccgt ctggcggttg
     2161 ccgcagatgt accgatggcc ccgtccaccg ctgatgatca tcttgcggcc gcacaggccg
     2221 cactcgagga gtcctgacag gagatatcgc gggtacgctc gcggcgaatc gtagccggtg
     2281 tcacgacgcg cttgcgctct ggcccacaaa tcgaggtcga tgatggcgag cccgtcccgg
     2341 acgatccatt ccgaccgcgg attctcaatc cgcttgcgct tcgagctgtc ggtggccgag
     2401 cgtatccagc ggcagcgatt ccacacgatg cggccgatgt agcgttcgtt gcgcaacatt
     2461 gcgtgaattg cactgacgcg ccatttaccg tccgtggcgc gctcgcggcg tttccaggcg
     2521 gcgccggggg agggtattcc tcgccggttc aggtccaccg cgatcgcacg ctgggtctcc
     2581 ccggcggcgt agcgctggaa tatctcaagg acgacaggcg cctggtcggg atcgatttca
     2641 tcgccggcaa acccgaaggt ctttccgccg gtgggtttct tctggcgagc acgaccctcc
     2701 agtccgcggc gcgtgcgcct cgcaatttct cgccggtagg cctcggccat cgccccactg
     2761 acggcaccga ggattgcggc cgactcctga cgcgtatcca ggtcgtgcgt caccacgtgg
     2821 atatcgagat cggcgagctc agccaggcgg ggcgcctgct cggcgagcaa acgccagagt
     2881 cgggaacaat cttcggccag gatgacttcg aactcgcgcc gccgtgccgc ttcgagcatg
     2941 gcctgatagc ctggccgatt gcttgtgccg ccgctgattg ccgcgtctga gaagcggcga
     3001 atgacagtga aaccgtgcgt cgcggccagt cgttcgcaga cgcgcagctg gtcctcgatg
     3061 gagctatcgc tttgcttgtc tgtcgaaaag cgggcgtaga gtgcggctct cacggggttt
     3121 ctcacttttc gggagtgtca cgcgctcgcg cgggctttgc aagcgccgat aggcaaccct
     3181 cgacaacaga tcaaccagcg ccgcgatgtc cggtcgcatg gcgctttcgc ttgctcgcgt
     3241 ggtcttgtcg gtcattggct ccctggcaat aagcttgaat ccacctacct gacgcatatt
     3301 ctggggagcg accgctcgtg ctgggactcg aaagcctgac aaaccggtgg cgggttttgc
     3361 gtcatcgccc caagtacacc acggagatcg atggccgatc atggcccagc tcgcgagcaa
     3421 tcgtttcgcg cgcaagttgg tcttgcgcgc ggtcatggat agaaaatgat ttcgacgctg
     3481 ggccgcccgc ggctggcgaa tcccagcccg tcaattcgcg gtagcgctgc tgggcgtagg
     3541 cgtggcgcag gccgtgcatg cgggagaggc ccgcccggat gcactggccc tcgtagagcc
     3601 ggagttgttc ggcgtaggtc ttctcggccg ggatgagcga cccggcgccg gccagggcct
     3661 tggcccggtc caggacctcc cgctgcgcct cggtccggat cgggacctcc cgggcccggc
     3721 cgcccttggt ccagctgggc ttgagccaga gccaatcgcc gttgtcggcg agccgcggcc
     3781 ggatcttgat ggcttcctcg cgccgaagcc cgaacgctgc ctggagttcc aggctcatcc
     3841 ggacgtgagg gtccgtgact ctctccaggt ttgtcttttc cagtgccttg gcacggttgg
     3901 cgccgttcgc atactgccgg cgcggcaaac cgtagtgatc gttcgagcgg gccactacat
     3961 tccgtttgtt gaccttgtcc gcccaccacc ggagcgcggc catgcggttt ttcatggtgc
     4021 ctgccgctac gcctgattcg agccaccggg tcgtcagggc ctcaacgtgc ttcggcttca
     4081 gggacttggc gtgcatttgc gtgaacccga gctcgtggag ctggttcgcg atgagcgtca
     4141 gcacccgttc ccgctgcacc tgggtgccgt agctgccgtc ccggttgtgt ttgcaaagct
     4201 gtttcagctg gtggttcagg cgtcgcaccg gtgtcagaac ccttaatgca gttccattcg
     4261 aagctggtaa gtgtttccga tcacttgccg accgtggtca gacaaggcca tccggttctg
     4321 gatgacttcg atcccgggac accactcccg tcctaacctg aacaggcctg gtcacaggcc
     4381 acaccgattc tctgccgcag cagaagggtt cggtgctaac cagctgatag ccagcggaac
     4441 gtggatgatc gaatgcattt ggcaaagtcc tccttgttgg gaaagtgcag ggcaatcacc
     4501 tgcaggtgga tgaaaatgcg ggaatgaccc gcggcgtttg acaatggact ggcggaggat
     4561 gaatccccgg tgctgacctg cagccctttc agcacgttgc cggaatatag cgaccggcga
     4621 cgctctcaat ggacgtagcc tacgacgtgg tcatgcgcgg ggcatcgaag aaacctaccg
     4681 gaacgccgcg ccatttaagg gctggcgccg cggggg
//
LOCUS       DASUOM010000585         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_802330,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000585 DASUOM010000000
VERSION     DASUOM010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802330"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFY27_12185"
     CDS             <1..892
                     /locus_tag="VFY27_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5766321.1"
                     /translation="GATAGADGRDDAERFDDLLWKPYKGLKDQDPGFLIYPVLGNHDW
                     DTSRVGAMAQVTYLERSPLYDLDGIFYRKRAAPDVEIFAIDTTVLLNGLPVYDDALGP
                     DGIPIDTGKFDKDEPWAEPVGEEERMLEWLERSLAGSDARWKVVIAHHPIWSSSGTKH
                     EEAKVLREVLLPMLCRYADAYIVGHDHTLELHSDDCRSAGNEYRNLPPLVQVVSGAAG
                     KQRPAHTPFMAWQDRTFPQKKTWYARGLVWGFVELQLMGDTATVTMVSTPNSGSGEPV
                     VEYSRSFERRSGESSKVAPQ"
     gene            943..>2294
                     /locus_tag="VFY27_12190"
     CDS             943..>2294
                     /locus_tag="VFY27_12190"
                     /inference="COORDINATES: protein motif:HMM:NF007790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HEX5766322.1"
                     /translation="MILVGFFGFTLGVALITWYATRRHEVTATPEGYFLGGRTLSAWV
                     IAGSLLLTNLSTEHLIGLNGDAFQHTIAVTAWETTAALAMVLTALVFLPRYLRAGLTT
                     IPDFLENRFDRHTRVIMSLLFLVSYAVAILPVVLLFGASGLETLFDVSTNLGISAGEA
                     RWLLVWGVGTLGSLYAIFGGLRAVAISDTLNAVGFFIGGLLVTFLALAAVGDGNVVAG
                     LQNVYTEEHDKFDITGNEPGSFLPFGVLFTGMIVNQVFFWCVNQSILQRALGARNLAE
                     GQKGVLIAAFFKLFAPIIIVLPGVVAWHMFKGTLAPSDYLLAYPSLVKAVLPAAAAGL
                     FAAVMVGAVLSTFNSVLNSAATLFSLGVYQGALNRKAEGRQLVVAGRTCSIVLAIAAM
                     IAAPSIDTSGSLYNYLQQINATFFGPMLAVILAALFTRRISAAAAKTALIAGPIVFYL
                     "
BASE COUNT          397 a          712 c          723 g          462 t
ORIGIN      
        1 gggagcaacc gcgggagccg acgggcgcga cgatgcggaa cgctttgacg acctcctgtg
       61 gaagccttac aagggactga aggaccagga tccgggtttc ctcatttatc ccgttctcgg
      121 aaatcacgat tgggatacct cgcgcgtggg cgccatggcg caggtgacgt atctcgaacg
      181 atcaccgctt tatgacctgg acggcatctt ttaccgcaag cgcgcggctc cggacgtgga
      241 gatttttgcg atcgacacga cggtgctgct gaacggcctg cctgtttacg acgacgcgct
      301 cgggcccgac ggcataccga tcgataccgg caagttcgac aaggatgagc cgtgggcgga
      361 accggtgggc gaggaggagc ggatgctcga atggctcgaa cggtcgctcg ccggctcgga
      421 cgcccgctgg aaagtcgtga tcgcgcatca tccgatctgg tcttcctccg gcaccaagca
      481 tgaagaagca aaggtgttgc gcgaggtgct gctcccgatg ctgtgccgtt acgccgacgc
      541 ctacatcgtg ggtcacgatc acacgctcga gctgcacagc gatgattgcc gcagcgccgg
      601 caacgaatac cgaaaccttc ccccgctcgt gcaggtggtt tccggcgctg ccggcaaaca
      661 acggcccgca cacacgccgt tcatggcatg gcaggaccgg accttcccgc aaaagaagac
      721 ctggtatgcg cgaggactgg tgtggggatt tgtggagttg cagctcatgg gcgacacggc
      781 aacggtaacg atggtctcga cgccgaactc cggtagcggc gagccggtag tcgagtactc
      841 gaggagcttc gagcgccgtt ccggcgagag ttccaaagtc gcaccgcagt gatcgcggca
      901 tgatgtacgg catcacctgg ccctgatttg cgcaagcact gaatgatcct cgtcggcttc
      961 ttcggattca cccttggcgt cgcgctcatc acgtggtatg cgacgcgacg gcatgaggta
     1021 acggcaacgc cggaaggtta ttttctcggc ggccgaacct tgagcgcctg ggttatcgcc
     1081 ggttcgctcc tgctgaccaa cctctcgacc gagcacctga tcgggctgaa tggcgatgcc
     1141 ttccagcaca ccatcgcagt gacggcatgg gagacgacgg cggcgctggc gatggtactc
     1201 acggccctgg ttttcctgcc gcgctacctg cgcgcggggt tgacgaccat cccggacttc
     1261 ctggagaacc gcttcgatcg gcacacccgc gtgataatgt ccctgctctt cctcgtttcg
     1321 tacgccgtcg ccatcctgcc cgtggtgctg ctgttcggcg ccagcgggct ggaaacgcta
     1381 ttcgacgttt ccacgaacct cggcatctcg gccggagagg cacggtggct cctcgtctgg
     1441 ggcgtcggaa cgctcggttc gctttacgcg atttttggcg gactgcgcgc ggtcgcgatc
     1501 tcggacactc tcaacgcggt gggcttcttc atcggcggtc tgctggtgac gttcctcgcg
     1561 cttgcggccg tgggcgacgg caacgtcgtc gcgggcttgc agaacgtcta caccgaggag
     1621 cacgacaagt tcgacatcac cggcaacgag cccggaagtt tcctgccgtt cggcgttctc
     1681 ttcaccggga tgatcgtcaa ccaggtgttc ttctggtgcg tgaaccagtc gatcctgcaa
     1741 cgcgcgctcg gcgcaaggaa ccttgcggag ggccagaagg gagtattgat cgccgccttc
     1801 ttcaagctgt tcgcgccgat catcatcgta ttgccgggcg tcgttgcatg gcacatgttc
     1861 aagggcacgc ttgccccgtc cgattatttg ctggcgtatc cctcgctcgt gaaggcggtg
     1921 ttaccggcgg cggcggcagg actgttcgcc gctgtcatgg tcggggcagt gctcagcacc
     1981 ttcaacagcg tgctgaacag cgccgcgacg ctgttcagcc tcggcgtcta ccagggtgcg
     2041 ctcaatcgca aagccgaggg gcgacagctc gtcgtggcgg gtcggacctg cagcatcgtg
     2101 ctggcaattg ccgcgatgat cgccgcgccg tcgatcgaca ccagcggcag tctctacaac
     2161 tacctgcagc agatcaacgc caccttcttc ggtccgatgc tcgcggttat cctggcggca
     2221 ttgtttaccc ggcgcatttc cgctgcggct gcgaaaacgg cactgatcgc gggccctatc
     2281 gtcttctacc tgat
//
LOCUS       DASUOM010000586         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_698245,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000586 DASUOM010000000
VERSION     DASUOM010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698245"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFY27_12195"
     CDS             <1..1091
                     /locus_tag="VFY27_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5766323.1"
                     /translation="VYSGVLNSGDTIYNPIKSKRERIGRLLQMHSNDRKEIKEVRAGD
                     IAAAVGLKDVTTGDTLTDPKSLITLEKMEFPEPVIAVAVEPKTKADQEKMGIALQKLA
                     KEDPSFRVHTDEESGQTIISGMGELHLDIIVDRMKREFKVEANVGKPQVAYRETIRKM
                     VEQEGKFVRQSGGRGQYGHVYLRIEPQEAGAGYEFVNAIVGGVVPKEYIGAVDKGVRE
                     QMENGVIAGYPVVDAKVTIYDGSYHDVDSSEMAFKIAGSMAFRDGAQKASPVLLEPIM
                     KVEVVTPEDYFGDVMGDLNRRRGLPQGMDEAPAGKIIRAEVPLAEMFGYATDLRSMSQ
                     GRATYSMEFEKYSEVPNNVAESVMKKAS"
     gene            1146..2336
                     /gene="tuf"
                     /locus_tag="VFY27_12200"
     CDS             1146..2336
                     /gene="tuf"
                     /locus_tag="VFY27_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5766324.1"
                     /translation="MSKGKFERTKPHVNVGTIGHVDHGKTTLTAALTKVMADKFGGEV
                     RGYDQIDNAPEEKARGITIATAHVEYESANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADQVDDAELLELVEMEVRDLLST
                     YEFPGDDTPIVTGSALKAFEGDTSEIGVPSVEKLIEEMDRYIPQPERAIDGDFLMPVE
                     DVFSISGRGTVVTGRIERGVVKVGDEIEIVGINDTSKTTCTGVEMFRKLLDQGQAGDN
                     VGVLLRGTKREEVERGQVLAKPGSITPHTKFEAEVYILTKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGAVELPEGTEMVMPGDNVQMVVDLIAPIAMEDGLRFAIREGGRTVGAGVVA
                     KIIE"
     gene            2501..2576
                     /locus_tag="VFY27_12205"
     tRNA            2501..2576
                     /locus_tag="VFY27_12205"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2534..2536,aa:Trp,seq:cca)
BASE COUNT          609 a          699 c          916 g          450 t
ORIGIN      
        1 gggtgtactc gggcgtcctg aactcgggcg atacgattta caacccgatc aagagcaagc
       61 gcgagcgcat cgggcgtttg ctgcagatgc attcgaacga ccgcaaggag atcaaggaag
      121 ttcgcgcggg cgacattgcc gcggcggtcg gcctcaagga tgtcaccacc ggcgatacgc
      181 tcactgatcc aaaaagcctg attaccctgg agaagatgga attcccggag ccggtgatcg
      241 cggtcgcggt tgagccgaaa accaaggccg accaggagaa aatgggcatc gcgctgcaga
      301 agctcgcgaa agaggatccg tcattccgcg tgcataccga cgaggagtcc ggccagacca
      361 tcatctccgg catgggagag ctgcatctcg acatcatcgt cgaccgtatg aagcgcgagt
      421 tcaaggtcga ggcgaacgtc ggcaagccac aggtggctta ccgtgaaacc attcgcaaga
      481 tggtggagca ggaaggcaag ttcgtccggc agtcgggcgg ccgcggccag tatggccacg
      541 tgtacctgcg catcgagccc caggaggctg gcgcaggcta cgaattcgtc aacgcaatcg
      601 tcggcggcgt ggtaccgaaa gaatatatcg gcgcggtcga caagggtgtg cgcgagcaga
      661 tggagaacgg cgtgattgcc ggatacccgg tcgtcgatgc gaaggtgact atctatgacg
      721 gttcctatca cgacgtcgac tccagtgaaa tggcgttcaa gatcgccggc tcgatggcgt
      781 tccgcgacgg tgcgcagaag gcaagccccg tattgctcga gccgatcatg aaggtggaag
      841 tggtcactcc cgaggactat ttcggcgacg tgatgggcga cttgaatcgc aggcgcggac
      901 tgccgcaggg catggacgag gcgccggccg ggaagattat ccgcgccgaa gtgccgcttg
      961 cggaaatgtt cggctatgcc accgacctgc ggtcgatgag ccagggtcgc gctacttatt
     1021 cgatggaatt cgagaagtac agcgaagtgc cgaacaacgt cgctgaaagc gtgatgaaga
     1081 aagccagcta gcaaaataag ggaacagatg tgaaatgtgt tccctgacca cccaggaacg
     1141 gagttatgtc gaaaggaaaa tttgagagaa cgaagccgca tgtaaacgtt ggcacgatag
     1201 gccacgtgga ccatgggaag acgacgctga cggcggcttt gacgaaggtc atggcggaca
     1261 agtttggtgg agaggtccgc ggctacgacc agattgacaa cgcgccggag gagaaggcgc
     1321 ggggcataac gatcgcgacg gcgcacgtgg aatacgagag tgcgaaccgt cactacgcgc
     1381 acgtggactg tccgggacac gcggactacg tgaagaacat gatcacggga gcggcccaga
     1441 tggacggtgc gatcctggtg gtgagcgcgg cggacggccc gatgccgcag acgcgggagc
     1501 acattttgct ggcgcgtcag gtaggggttc cgtacatcgt ggtgtacctg aacaaggcgg
     1561 accaggtgga cgatgcggag ttgctggaac tggtggagat ggaggtgcgt gaccttctgt
     1621 cgacctacga attcccgggc gacgacacgc cgatcgtgac cggttcggcg ctgaaggcgt
     1681 tcgagggcga cacctcggag atcggtgttc cctcggtgga gaagctgatc gaggagatgg
     1741 accgctacat tccgcagccg gagcgcgcca ttgacggcga tttcctgatg ccggtggagg
     1801 acgtgttttc gatctccggg cgcggcacgg tggtgaccgg gcgtatcgag cgaggcgtgg
     1861 tgaaggtggg cgacgagatc gagatcgtgg gcatcaacga caccagcaag acgacctgca
     1921 cgggcgtgga gatgttcagg aagcttttgg accagggtca ggcgggcgac aacgtgggcg
     1981 tgctgctcag gggcacgaag cgcgaggaag tggagcgagg ccaggtactg gcgaagccgg
     2041 gctcgatcac gccgcacacc aagttcgagg cggaggtgta catcctgacc aaggaagagg
     2101 gcggacgtca tacgccgttt ttcaagggct accgtccgca gttttacttc cgcaccacgg
     2161 acgtgaccgg tgcggtggaa cttccggagg gcaccgagat ggtgatgccg ggcgacaacg
     2221 tgcagatggt ggttgacctg atcgcgccga tcgccatgga agacggcttg cgcttcgcca
     2281 tccgcgaagg cggccgcacc gtaggcgcag gcgtcgtcgc aaagatcatc gagtaagcaa
     2341 atcggggtca gaccccatca caaaacgacc tacgacctat gaacaaaggg cgatgcgatt
     2401 gcggcttctg ccaccgatcg gatcattagc attgcaacgt tgcgacccga cagctgtcga
     2461 caaaaacgtc acgctcgtca ggttggtacg accaaaggat aggccagtag ctcaattggc
     2521 agagcggcgg tctccaaaac cgcaggttgg gggttcgatt ccctcctggc ctgccatttg
     2581 agcaagagcc tcgcttggca ggttctggct aaggcgaagc tggactgaac tcgatattga
     2641 tgaacgcaca gacggaagcg ggcgaatccg gggt
//
LOCUS       DASUOM010000587         7994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1041599,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000587 DASUOM010000000
VERSION     DASUOM010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041599"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFY27_12210"
     CDS             <1..230
                     /locus_tag="VFY27_12210"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX5766325.1"
                     /translation="QFLQYLRKGESLSVDMPDGRGHLFRVVDIDIVDSRRGSIQLDTD
                     TPMLSLVTCYPFNARDRGGPMRYVVTAAMVY"
     gene            282..1001
                     /locus_tag="VFY27_12215"
     CDS             282..1001
                     /locus_tag="VFY27_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330228.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5766326.1"
                     /translation="MTTPSTTSAAVLGGFLFAGLVALALILGNAALDVKAHERSVQVK
                     GLAEREVPADVVIWPLSYQLASNDLNDLYESIAEKNGIITRFLADNGLDLADVSTAAP
                     TVVDRHAQSWGNTSEIEYRYVATATVTLYSSDVDTARKAMANAIDLGKNGIALSGEQY
                     GNQVQFMFSGLNDIKPTMIEESTFNARAVAQKFAADSESTLGKIKSAQQGQFSITDRD
                     STTPHVKNVRVVSTVEYYLSD"
     gene            complement(952..1530)
                     /locus_tag="VFY27_12220"
     CDS             complement(952..1530)
                     /locus_tag="VFY27_12220"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5766327.1"
                     /translation="MSWKKLSSKVVFDNPWITVFEDRVINPGGGRNDYGHVHFKNKAV
                     AVLPLDDRGNTWLVGQDRYTLGTFTWELPMGGAPLAESPLDAARRELREETGLIAARW
                     TEVMRLHTSNSITDEVGIVFVAEELTEGPSDLDETENIELRKLPLAEALDMIDRGEIT
                     DAISVAALLGVARRLEPSRRGSTPPSIPRERS"
     gene            1712..3091
                     /locus_tag="VFY27_12225"
     CDS             1712..3091
                     /locus_tag="VFY27_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5766328.1"
                     /translation="MASRQARLTQGPVGRHLVDMTVPVLFGVFTMMMQAFVDAWFIGQ
                     VGDRELAALGFAFPVLMLVSSVAIGLGAGTSSVVARALGAEDYDRARRLTTDSLLLAF
                     GMTALVAVAGILTIGPLFRLLGAPEDLIPLIGGYMRILYAGVPFVVVGMVGMSSMRAT
                     GDTRLPSILMVLASVLNVVLDPLLIFGFGPIPRMELDGAAMAAFLARGSLFFGALYFM
                     RRRLDMLSFDRPDPVELRKSWVDILHVGIPAAGTNAIVPIATGAITAMLARYGPEAVA
                     GFGVASRVESLMLVIYYAMSAIIGPFVGQNVSSGKADRIYLALRLCTLFCLASGLVIA
                     LALAASSTFLPSLFSDNPAVTRVATTFLLIAPLGYGAYGMVMVMNAAFNGMGKPIPGV
                     WISMMRLAALYLPLAFIADRFFGVTGIFAAYTVANIVTGITAYLWARSAVQSQVDKHG
                     PRIMATEIV"
     gene            complement(3120..4865)
                     /locus_tag="VFY27_12230"
     CDS             complement(3120..4865)
                     /locus_tag="VFY27_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5766329.1"
                     /translation="MAFRTAIVSAEPVAGSAGSSLESDEFVVERDVPVQMRDGVKLHA
                     DVFRPRGGGPFPVLVYRTPYGKSAAADAYDTHVDAVRRGYAVVLQDVRGRYSSDGNFD
                     PYRREGLDGYDTIEWAAARPWSNGLVGTYGLSYPGAVQWLAAMESPPHLKAMAPAMTF
                     SSPRNFFYSNGTFDMSWLPWIYQNVAPDRRVREGLPGIASGEEARQSWPAVAQEYLSW
                     RPLRALPYLRREAPFYFEWLEHPPEDPWWDWAELRSRYGTVTAAVLNLSGWFDEAYGP
                     EGAITNFNGLTAARSGDANERTHLILGPWTHGVHATMSRRAGELDFGPQAVIDYDDVL
                     LDFFDRYLRYDDHHAAPTVRYFVMGENRWREDTQWPPADMVSGTRYLVAADEKLLLSP
                     HPEPNDAASSKFVSDPDLPVVNPHPLLGPHDYQHLAERKDILTFDTEPLKASLTVAGD
                     IAAIVYASCDCRDFDLWVRLQDVRPDGRALSLMSPGNDVQRASYRDPAAGRQLLQPGE
                     VYEVKLSKLSTAVRFAKGHRLRVQISASFAPNFSTNLQTGLSEIDSAASRPATISIHH
                     DLKYQSRLILPVVHP"
     gene            5034..6044
                     /locus_tag="VFY27_12235"
     CDS             5034..6044
                     /locus_tag="VFY27_12235"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX5766330.1"
                     /translation="MYVFFVHWPAALSALALLMISGLASAGPAYRHYTAGNVEDVVTP
                     TSGLLVLQGGGTDVDENYVEMGRAGGGGDFVVLRASGSDEYNAYIHALCACDSVETLV
                     FEDRQAASDPRVIEIIRNAEALFIAGGDQSRYVRFWKDTAVEDAINYVAAKPAPVGGT
                     SAGMAILGEFAYSAMSEESLTSGTALGDPFHEDLTLSREFVDVPVLKNIITDQHLQER
                     NRIGRTVALLARLMNDGWTKEARAIAADRETALHIDPATGAARVFATKHETPHVWFMR
                     AQTRPLRCEPRTPLTFREVAVYRIGPGGSFNVNSWSGREGLAYTLNVDRGVLSSTRGD
                     VY"
     gene            complement(6038..7186)
                     /locus_tag="VFY27_12240"
     CDS             complement(6038..7186)
                     /locus_tag="VFY27_12240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5766331.1"
                     /translation="MHQADLRNTHDPSASRWPKLVVTAVLLVLAAGCAGVPQGASKQV
                     DLLFAPLATGVQPGAAVMVIRRGRIVHQAGYGYADLERRVPITADSAFNLASVSKQFT
                     AMAVMVLAASGDLGYDDPVSQYLPQLSPYKGVTIRQLITHTGGLPDYYDIIDTRYGMP
                     RNSEALTLLGDMATPDFPPGERFAYSNSGYDMLGPLVEAVSGTDFATFMRERVFAPAG
                     MRHSLIHDHTLPMIASRAYGYEPIGDGFRLYDDDPLNGIVGSGSMYSTLNDLFRWDQA
                     LYTDTLLGRSALDEAFVSARLNDGTAVDYGFGWRLDEYDGRRRVRHGGSWVGFRTHIA
                     RYPDERFTVVILANRADLQPEIYIDAITDIYLSSQGGEAQKQAGTARQ"
     gene            7588..>7994
                     /locus_tag="VFY27_12245"
     CDS             7588..>7994
                     /locus_tag="VFY27_12245"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5766332.1"
                     /translation="MTPLAAAIFAVLNPVTSLAQEQQQEQGLEEVIVTATRRESNLQE
                     VPQSITVFSTEDIQRQAFQNMQDYIKALPSMNLVNVMPGRNSVIMRGVSTGSAEYRTD
                     SQVAVYLDEQPMTSISQQVDVRMIDVARIESLPG"
BASE COUNT         1520 a         2433 c         2499 g         1542 t
ORIGIN      
        1 tccagttcct gcagtacctg cgtaaaggcg aatcgctgag cgtcgacatg ccggacggtc
       61 gcggtcatct gttccgggtg gtcgatatcg acatcgtcga ctcgcggcgt ggcagcatcc
      121 agctcgatac ggacacgccg atgcttagcc tggtgacctg ctatcccttc aatgcgcgcg
      181 acaggggcgg cccgatgcgc tacgtcgtca ctgccgccat ggtctattga cgttgctacg
      241 tccgggggcg agcatgctcc atcacaaaag ggaccggatg tatgacgaca ccatcaacga
      301 cttctgcagc ggttttgggc gggttccttt tcgccgggct tgtagcgctg gcgctgattc
      361 tcggcaacgc cgccctggac gtgaaggcgc acgagcgaag cgtgcaggtg aagggactgg
      421 ccgagcggga ggtgccggcc gacgttgtca tctggccgct ttcgtaccag ctcgcgagca
      481 acgacctgaa cgatttgtat gaatccatcg cggagaagaa cgggatcata acccgcttcc
      541 tggccgacaa cgggctcgac ctggcggacg tctctacggc tgcaccgacg gtggtcgacc
      601 ggcatgcgca atcctgggga aatacctcgg agatcgaata ccggtatgtc gcaacggcaa
      661 cggtgacgct ttattcgagc gacgtggaca ccgcgcggaa ggccatggcg aacgcgatcg
      721 acctgggaaa gaacggaatt gccctgagcg gcgagcagta cggtaaccag gtgcagttca
      781 tgttcagcgg tttgaatgat atcaagccca cgatgatcga ggagtcgaca ttcaacgccc
      841 gcgcggttgc gcagaagttc gccgcggatt cagagagcac gctcggcaag atcaaatccg
      901 cccagcaggg ccagttttcc atcaccgacc gcgacagcac tacgccccac gtcaagaacg
      961 ttcgcgtggt atcgacggtg gagtactacc tctccgacta ggttccaggc gccgcgccac
     1021 gcccaacaga gccgcgacgc tgatcgcgtc cgttatctcg ccacgatcga tcatgtccag
     1081 cgcttcggcg agaggaagct ttctcagctc gatattttct gtctcatcga gatccgacgg
     1141 cccttccgtc aactcttccg ctacgaacac tatgccgact tcgtcggtaa tcgagttgct
     1201 ggtgtgcagc cgcatcacct ccgtccacct cgcggcgatc agtcctgtct cttcccgcaa
     1261 ctcccgccgc gccgcgtcca gcggcgattc ggcgagcggc gcgccgccca tcggcagttc
     1321 ccaggtaaaa gtgccgagcg tgtagcggtc ctggcctacc agccacgtgt ttcctctgtc
     1381 gtcgagcggc aggaccgcca ccgccttgtt cttgaagtgc acatgcccgt agtcgttccg
     1441 gccgccgccg ggattgatga cgcggtcctc gaacacggtt atccagggat tatcgaaaac
     1501 gactttcgag gataattttt tccaggacat ggttacgcat cgcgagcttg caaacagcgc
     1561 gcatgataac ccgaaggcgc cagcgatttg cctgcaccca gcggattgca ggtcgatcgg
     1621 cgttccgtca tagttatgcc gcttcagccg ttgccgggct ctgccccgaa cctgcgcggc
     1681 aatccgctaa ccccttgttc cccgggacac catggcatcc agacaggccc gactgacgca
     1741 gggaccggtc ggccggcatc ttgtcgacat gacggtgccg gtcctgttcg gcgtcttcac
     1801 gatgatgatg caggcgttcg tcgatgcctg gttcatcggc caggtgggcg atcgggaact
     1861 cgcggcgctc gggtttgcgt tcccggtgct gatgctcgtc tcgagcgtcg cgattggcct
     1921 cggcgccggg acgtcttccg tcgtggcgcg cgcgctcggg gcggaagact atgatcgcgc
     1981 acgccgcctg acgacggaca gcctgttgct cgcgttcggg atgacggctc tcgttgcggt
     2041 tgccggcatc ctgacgattg gcccgctctt ccggttgctc ggggcgcccg aggacctgat
     2101 tccgctgatc ggcggctaca tgcggatcct ttacgccgga gtgccgttcg tcgtcgtcgg
     2161 catggtcggc atgagcagca tgcgcgcgac cggcgacacg aggctgccga gtatcctgat
     2221 ggtgctcgct tcagtcctga acgtcgtgct cgaccctctg ctcattttcg gtttcgggcc
     2281 gatcccgcgg atggagctgg atggcgctgc gatggcggcg ttcctggcgc gaggcagcct
     2341 gttcttcggc gctctttact tcatgcgccg gcgcctcgac atgctcagct tcgaccgtcc
     2401 ggatccggtt gagcttcgca agtcgtgggt ggatattctt cacgtaggca tcccggccgc
     2461 gggcacaaat gccatcgtcc cgattgccac gggcgcgatc accgcgatgc tcgcgcgcta
     2521 cggacccgaa gccgtggcgg gcttcggggt cgccagtcgc gtggagtcgc tgatgctggt
     2581 catctattac gcgatgtccg cgatcatcgg accttttgtc ggacaaaacg tatcctcggg
     2641 caaagccgac cgcatttatc ttgcgctccg cctttgcacg cttttctgcc tggcgtcggg
     2701 gctggtcatc gcactggcgc tggcggcatc gagcacgttc ctgccatccc tgttcagcga
     2761 caaccctgcg gtaacacggg tcgcaacaac gttcctgctc atagcgccgc tcgggtacgg
     2821 cgcctacggc atggtgatgg tgatgaacgc cgctttcaat ggaatgggca agccgatacc
     2881 gggagtctgg atcagcatga tgcggctcgc ggcactatac ctgccgttag ccttcatcgc
     2941 agacaggttc ttcggcgtta cggggatttt cgccgcgtac acagtggcaa atatagttac
     3001 gggtattacc gcctacctct gggccaggag cgccgtccag tcgcaagtcg acaaacacgg
     3061 gccgcgcatc atggccacgg agattgtgta gccaggcagc cgcacttcag gaccgggctt
     3121 caggggtgca caaccggcag gatgagccgt gactggtatt tcaggtcatg gtggatgctg
     3181 atcgttgccg gtctcgatgc ggcagagtca atttccgaca gtccggtctg cagattcgtc
     3241 gagaagttcg gcgcgaatga cgccgaaatc tgcacccgca ggcgatggcc cttcgcgaaa
     3301 cgcaccgcgg ttgaaagctt cgatagcttc acctcgtaga cctcgccggg ctgcagcagt
     3361 tgccgccccg ccgcaggatc gcggtaactg gcacgttgta cgtcgttgcc gggactcatg
     3421 aggctcaggg cacgtccgtc cggccgaacg tcctgcaacc tgacccaaag atcgaagtcc
     3481 cggcaatcgc aggaagcgta gacgatcgcg gcaatgtcac ccgcaaccgt caggcttgcc
     3541 ttaagcggct cggtgtcgaa ggtaaggatg tccttacgct ccgcgagatg ctgatagtcg
     3601 tgcggcccga gcaacggatg cggattgact acggggagat ctggatcgct gacgaatttg
     3661 ctggatgcgg cgtcgttcgg ctcagggtgc ggagaaagga ggagcttttc atcggccgcg
     3721 acgagatacc gcgttccgga aaccatatcg gcgggtggcc attgagtgtc ttcgcgccag
     3781 cgattctcgc ccattacgaa gtagcgaacc gtaggggcgg cgtggtgatc gtcgtatcgc
     3841 aggtagcgat cgaaaaagtc cagcaagacg tcgtcgtagt cgatcactgc ctggggtccg
     3901 aagtccagct caccggcacg tcgcgacatg gtcgcgtgaa cgccatgcgt ccagggtccg
     3961 aggatcagat gggtgcgctc gttcgcatcg ccggagcgag cggcggtcag gccgttgaaa
     4021 ttggtaatcg cgccttccgg cccgtatgcc tcgtcgaacc agccgctcag gttcagtacg
     4081 gccgccgtga cagtgccata gcggctacga agctccgccc agtcccacca gggatcttcc
     4141 ggcgggtgct caagccactc gaagtagaac ggcgcctctc tccttagata aggaagcgct
     4201 cgtagcggtc gccacgacag gtattcctgt gcgaccgctg gccacgattg acgggcttct
     4261 tcgccactcg caatgccggg caggccctcc cgcacgcgcc tgtccggcgc cacattctgg
     4321 taaatccagg gcagccagga catgtcgaac gtgccgttgg agtagaaaaa gttgcgaggg
     4381 gacgagaagg tcattgccgg tgccatggcc ttcaggtgcg gtggcgactc catggcagca
     4441 agccactgga cagcgcccgg atacgacagg ccgtaggtcc caaccaggcc gttcgaccag
     4501 ggtcgggctg cggcccattc gatcgtgtcg tagccatcaa ggccttcccg ccggtacgga
     4561 tcgaaattcc cgtcggacga gtagcgcccc cgaacatcct ggagcacgac agcgtagccg
     4621 cgccggacgg cgtcaacgtg cgtgtcgtaa gcgtcggccg cggcgctttt cccatacgga
     4681 gtgcgataaa cgagcacggg aaaagggcct ccgccgcggg gccggaaaac gtcggcatgg
     4741 agcttgacgc cgtcgcgcat ctgcaccggg acgtcgcgtt caacgacgaa ctcgtcagac
     4801 tcgagggagg atcccgccga tccggcaacc ggttccgcag agacgatggc tgttcggaaa
     4861 gccactgcgg ccatgatgaa gatgacggag tagcgtcgca ttcgatgatg gtcgtactgc
     4921 gggtgaggaa aatcacgcgg ggttcacatg atggatatgc tgccgtccgc gggagcgatt
     4981 gttggctaag ctttccaggc tgccagcaga ggcgccttgg agtgcgaata cagatgtacg
     5041 tgttctttgt gcattggccg gcggcgttat cggccctggc actgctcatg atcagcggac
     5101 tggcgtcagc cgggccggct tatcgccact atacagcggg aaacgttgag gatgtcgtta
     5161 cgccgaccag cggtttactg gtcctgcagg gcggcggcac cgatgtggat gagaactacg
     5221 tggagatggg gcgggctggc ggcggtggcg attttgtggt gctgcgggct tccggaagcg
     5281 atgagtacaa cgcctatatc catgcattat gtgcatgcga ttcggtcgag accctcgtct
     5341 tcgaggacag gcaggcagca tccgatccgc gtgtcatcga gatcattcgc aatgccgaag
     5401 cgctcttcat tgcgggcggc gaccagtctc gctatgtccg tttctggaaa gacaccgccg
     5461 tggaagatgc aatcaattac gtcgccgcga aacctgcgcc cgtcggaggt accagtgccg
     5521 gaatggcgat actcggggag ttcgcgtatt cagcaatgag cgaggaaagc ctgacatcgg
     5581 gaacggcact cggtgacccg ttccacgagg atttgacgct ttcccgtgaa tttgtggatg
     5641 tcccggttct caagaacatc attacagatc agcacctgca ggaacggaac cgcatcggcc
     5701 ggacagtggc gctgctcgcg cggctcatga atgacggctg gacgaaagag gcccgcgcga
     5761 ttgcggcgga ccgggagaca gcgctgcaca tcgaccccgc gacgggagcg gcaagggtct
     5821 ttgctacgaa gcacgagaca cctcatgtct ggttcatgag agcacaaacc cggccgctgc
     5881 ggtgcgagcc gcggactcca ctgacgttcc gcgaggtcgc ggtataccgg atcggcccgg
     5941 gaggcagctt taacgtcaac agctggagcg gccgggaggg cctggcatat acgctgaacg
     6001 tcgatcgggg tgtcctctca tcgactcgag gcgacgttta ctgacgagca gtccccgcct
     6061 gcttttgcgc ttcgccgcct tgcgatgaaa ggtaaatgtc cgtgatcgcg tctatgtaga
     6121 tctccggctg caaatccgct cgattggcga ggatgacgac ggtgaaccgc tcgtccgggt
     6181 agcgtgcgat gtgggtcctg aaaccgaccc agctgccgcc gtggcgcaca cgccgtcggc
     6241 cgtcgtattc gtcgagcctc cacccgaagc cgtagtcgac cgctgtgcca tcgttcagcc
     6301 gtgccgatac aaatgcctca tcgagtgcgc tgcgaccaag tagcgtatcg gtataaagag
     6361 cctggtccca cctgaacagg tcgttgagcg tcgagtacat gctgccggag ccgacgatac
     6421 cgttcagggg atcgtcatca tacagcctga aaccatcgcc gatcggctca tagccgtacg
     6481 cgcgcgatgc aatcatgggc agcgtgtgat catggatcag ggagtggcgc ataccggcgg
     6541 gcgcgaagac tcgctcgcgc ataaacgtgg cgaagtcggt gccggacacg gcttcaacca
     6601 gcggcccgag catgtcgtac cccgagttgc tgtatgcaaa gcgctcgccg ggcggaaaat
     6661 cgggtgtcgc catatcgcct agcaaggtca gcgcttcgga gttgcgcggc atgccgtacc
     6721 tcgtgtcgat gatgtcgtaa tagtcaggca ggccgccagt gtgggtgatg agttgccgga
     6781 tcgtcacccc tttgtacggc gacaattgcg gaagatactg gctgaccggg tcgtcgtagc
     6841 cgagatctcc cgacgccgcg agaaccatga ccgccatcgc agtaaactgc ttcgacacgg
     6901 aggcgagatt gaaagcggaa tcggccgtaa tcggaacacg cctttccaga tccgcgtagc
     6961 cgtacccggc ctggtggacg attcgtccgc gccggatcac catgaccgcc gcccccggct
     7021 ggacgcccgt cgccagcggc gcgaacaaca aatcgacctg ctttgaagcc ccctgcggca
     7081 ctcctgcgca gccggcagcc aggaccagaa gaacggccgt gactaccagc ttcggccatc
     7141 ggctcgctga ggggtcgtga gtatttcgca aatctgcctg gtgcatggat ttcgtccgga
     7201 agctcaacga tcgggggcgg cagcccgtga gcctccaacg ctcacaggac gcgtcagacc
     7261 gcgggtcata tgctgcaaaa gtctcagggc ctgtcggcga ggaaaccaca cagtgaaacg
     7321 ccaggcaaat tcctgcttta ccagttcgca cgctgccgaa ggcctagtga taatggtcat
     7381 ttcaccatgt gatgcatgcc tactaatcac tcattgttca gagcaaagtc ttgggttcgc
     7441 tcccgcaccg ctttccgccg gtggaaggag cgccgatcat tgcaaccact cgggggagaa
     7501 cgcagtatga tcaacgcgcc agcgaccgca gaatcctgca gttcacgaat tgacggcgct
     7561 cctgcccccg gcttacgcag ttttcgcatg acaccgctgg ccgcagcgat tttcgcggtg
     7621 ttgaacccgg tcacgtcgct cgcacaggag cagcagcagg aacaggggct cgaagaagtc
     7681 atcgttacgg cgacccgccg cgaatccaac ctgcaggaag ttccgcaaag catcacggtg
     7741 ttctcaaccg aggatatcca gcggcaggcg ttccagaaca tgcaggacta catcaaggcc
     7801 ctgcccagca tgaacctggt caacgtcatg ccaggacgta actcagtcat catgcgcggc
     7861 gtctcgaccg gttccgcaga gtaccgcacc gacagccagg tggccgtcta tctcgacgag
     7921 cagcccatga cctcgatctc gcagcaggtc gacgttcgca tgatcgatgt cgcgcgcatc
     7981 gagtccctgc cggg
//
LOCUS       DASUOM010000588         9295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_564133,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000588 DASUOM010000000
VERSION     DASUOM010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564133"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFY27_12250"
     CDS             <1..183
                     /locus_tag="VFY27_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766333.1"
                     /translation="VIGPANPAYRLSPNELRRSVTAVATRRDEVLYYREGRVTDPDGR
                     EAALAQLVLRRQLDRS"
     gene            185..631
                     /locus_tag="VFY27_12255"
     CDS             185..631
                     /locus_tag="VFY27_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX5766334.1"
                     /translation="MQPRRLKTLSYLMDESIRLPGGYRIGWDGIIGLVPGLGDVAGLA
                     VSAYIIAESARLGAPKSVLLRMSANTAIDTILGSIPLLGDLFDLAFKANRQNLRLLQS
                     HLDAPQRTRHVSIGWLVIVGLVALLGLATVLVLLSRLVAWLWNLIF"
     gene            736..1521
                     /gene="ygiD"
                     /locus_tag="VFY27_12260"
     CDS             736..1521
                     /gene="ygiD"
                     /locus_tag="VFY27_12260"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX5766335.1"
                     /translation="MQNSTTMPVIFFGHGNPMNAIEDNPWAREWSAIGAAMPRPRGIL
                     SISAHWYLPLTAVTAMQQPRTIHDFGGFPRALFEVQYPAPGSPDLAQRVSQLLAPAQV
                     AMDDRWGLDHGTWSVLAHAWPEADIPVVQLSIDDTRSAGWHYALAKRLAPLREDGILI
                     MGSGNLVHNLHTYAWGERAAKPYEWAVRFEAMAREQLERGDFAPLIAYETLGRDAMLA
                     APTPDHYLPLIYVLAQYRPGEAVHFPVEGFDGGSISMLSVQFG"
     gene            complement(1539..3326)
                     /locus_tag="VFY27_12265"
     CDS             complement(1539..3326)
                     /locus_tag="VFY27_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766336.1"
                     /translation="MPFTPDGSKPAPATRHQLRRALSFTVPHRLTIVTILLLTLAVSV
                     VSAIEPLVMKYIVDGLTADGGMEVVWLGLVFLGILALCRDGAGGTSNWLTWRIRVRLQ
                     YALLEATVGRLHRMPLKMQRSEGVGAILTKLDRSIQGFVAAVTQLLFSVLPALVFLIM
                     AIAIMVDLEWRLALLVLVFAPLPAVLAAVAAPEQTRRERNMLDRWAQIYSRFNEVLAG
                     LVTVRSFAMEEAEKDRFLKGVNEANRVVERGVATDTAFGTASNLVVAIARIAVIGVGG
                     ALVVRGRMTVGTLIAFLGYVGGLFGPVQGLSATYQSLQRATVSLGQIFSILDLQEHLG
                     DSPEAHDVEAVRGDIEFIDVHFSYGPAERPLLNGISLKVAAGETLAVVGPSGSGKTTL
                     MALLMRFYDPDSGAVLLDGQDLRTLKQSSLRRHIGVVLQDPLLFNDTVSANIAYGRPE
                     ASQPEIEQAARAASAHDFIMLLPEGYKTVVGERGARLSTGERQRISIARALVKEPSIV
                     VLDEATASLDAESEASVQAALDTLITDRTTFVIAHRLSTVVSADRIVVLQNGRIAESG
                     SHQQLLGSGGYYAQLVRQQTRGLIRNEGE"
     gene            3580..5265
                     /locus_tag="VFY27_12270"
     CDS             3580..5265
                     /locus_tag="VFY27_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008897573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5766337.1"
                     /translation="MPAEEFDAIVVGSGISGGWAAKELTEKGLRVLLLERGKNVEHIK
                     DYVNARKAPWEFPHRGGRTHAMEEAYPVLKRDYPLNEKNLDWWASDADSPYTEVKRFD
                     WFRGYHVGGRSLMWGRQSYRWSDYDFEANARDGVAIDWPIRYADLAPWYDHVERHAGI
                     SGSQEQMPQLPDGQFQPAMPLNCGEELVAGRLKGLFDGRRRIIPGRVANLTEALPGRA
                     PCQYRNACWLGCPYGGYFSTQSSTLPAAVATERLTLKPFAIVTEVLYDRDRRRASGVR
                     VVDAVTEHSTEYKAPVIFLCASTLNSTWILMRSATDVWPDGLGSSSGELGHNLMDHHF
                     RLGASGKLAEASLAGRYYYGRRPNGFYIPRYRNLFGDKRPYLRGFGYQGSASRNDWQR
                     AVGELGIGAEFKDAMSQPGQWEIGATAFGEMLPNHDNRASIDSSRTDKWGLPVLKIDC
                     ATGENERLMGIDMKNDMAEMLEAAGARDVEPYDRGYFPGMGIHEMGTARMGRDPRRSV
                     LNEWNQVWDAPNVFVTDGACMTSSACQNPSLTYMALTARAADYAVKELKRRNL"
     gene            5282..5884
                     /locus_tag="VFY27_12275"
     CDS             5282..5884
                     /locus_tag="VFY27_12275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5766338.1"
                     /translation="MSADSQRPDLITRREAILRVSALLGGAALVGQTAMLAGCAREGV
                     QSQAFSADDMALLDEIAETILPETGTPGAKAAGVGPFIAMMVVDTYDEREQEVFRAGL
                     QALEDECRAMHGMSFLAATPGQRTVLLRKLDAEQLQFMQTAGKHEPAHYFRMLKELTL
                     LGYFTSEIGYTQAMRYIETPGRYDPCVPWEPGEKTWAVHA"
     gene            5877..7025
                     /gene="proB"
                     /locus_tag="VFY27_12280"
     CDS             5877..7025
                     /gene="proB"
                     /locus_tag="VFY27_12280"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5766339.1"
                     /translation="MPEAPASASLFREGSFRRLVIKIGSSLLTDGSGAVNRPWLSGIA
                     EDITALRARDCGVLVVTSGAIAIGCRAIGINRRRARLEMLQAAAACGQVQLMQAYQEA
                     LGRHGIAVAQVLLTLDDTEHRRRFLNARGTLEKLLEHDVVPVINENDTVATDEIRYGD
                     NDRLAARVAQMVMADGLLLLSDIDGLYTSDPRENPDAEHVAGIGDISDDVIAMAGGPR
                     SADGSGGMATKLQAAQIATHAGCRTIIANGNGPRPVLALASGARCTMFRAQGTPASVR
                     QQWLAGALRTRGALHLDAGAVAALQEGSSLLPVGVTGVTGDFARGDPVALLNGDGREI
                     GRGLIAYESSEAARIIGCHSDELEARLGYRGRSVMIHRNDLVFFGRTK"
     gene            7057..8316
                     /locus_tag="VFY27_12285"
     CDS             7057..8316
                     /locus_tag="VFY27_12285"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5766340.1"
                     /translation="MNIGPMMCEMGRAAKLASQALGAAGTAARNVALQECATAIRNGA
                     VAILDANRQDLERARKLELGSAMTDRLALDESRVEAMAKGLEAIAALPDPLGRVIAGW
                     QRPNGLKIERVSVPLGVIGIVYESRPNVTADAAGLCIKSGNAVILRGGSESFLSSSAI
                     YACLQEGLKTAGLPLESVQMVPTTDRAAVGYMLSSMREYIDVLVPRGGKRLIARVQED
                     ARVPVIGHLEGICHVYLHASADQAMAQDIVLNSKMRRTGICGAAETLLVDRGAAERML
                     PPIIAALCEAGCEVRGDGMVQSISGSVLPATESDWSTEYLDAIISVRTVEDVDDAIRH
                     IRRYGSGHTEAIVAEDSTAAEHFLRGIDSAILLHNASTQFADGGEFGMGAEIGIATGK
                     IHARGPVGAEQLTSYKYVVRGAGQVRP"
     gene            8357..>9295
                     /locus_tag="VFY27_12290"
     CDS             8357..>9295
                     /locus_tag="VFY27_12290"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766341.1"
                     /translation="MNRSKNRSVPFMLIAAALTGIAACNGTPPDSVEGTTKSGPGWKT
                     VNDGVVVSFDNAEFRRVRLQVVDDGIIRVTATPQEDFSNLPDTLMIVAGAPDAGFRAE
                     QRGNEVVVQTGALEARVSLATGTVRFTDSDGNELLAEAERSLAPVTADPGATDDDSFF
                     VRQQFQRATDEDEGIYGFGQQQDGRVNYAGRNVELTTHNIEIAIPFFASSRGYGVLWN
                     NTSITRFGDPEPPRPLSAGFRLFDANGEPGGLTARYYAGGELKLEQVEPDLNYQFLSH
                     ANVREIPLPEEVRDSDDVRIEWEGSFVPSNDGIHELK"
BASE COUNT         1801 a         2834 c         2991 g         1669 t
ORIGIN      
        1 gtgatcggcc cggcgaatcc ggcgtaccgt ctttcgccaa acgaactgcg ccgttcggtg
       61 actgccgtcg ccacgcgcag agacgaggtg ctgtactacc gcgagggcag ggtaaccgat
      121 cccgacggtc gcgaagctgc gcttgcgcag ctggtgctgc gccggcagct ggaccgcagc
      181 tgaaatgcag ccgcgtcgcc tgaaaaccct ttcatacctg atggacgagt cgatcaggct
      241 gcccggcggc tatcgcatcg ggtgggacgg gatcatcggc ctggtgcccg gacttggaga
      301 cgtggcaggc ctggccgtgt ccgcttatat catcgccgag tcggcacggc tgggtgctcc
      361 gaagagcgta ctgttgcgca tgtcggcaaa taccgcgatt gacacaatct tgggctctat
      421 accccttctt ggcgacctgt tcgacctggc cttcaaggcg aaccggcaga acctgcggct
      481 tctgcagtcg cacctcgacg ccccgcagcg gacccgccac gtcagcatcg gctggctcgt
      541 tatagtcggg cttgttgcct tgctgggcct ggccacggta ctggtgctgc taagccgcct
      601 ggtcgcctgg ctctggaatc tcatcttcta gggaaggcct tgcaccagcc gccctttact
      661 ttataacgga gcctctgcgc atgtcggttc aaaggcacct gtagtagcac cttcttctcc
      721 gtagaaatca ttcctatgca gaattccaca acgatgcccg tcatcttctt cggccacggg
      781 aacccgatga acgcaatcga ggacaacccc tgggcacgag agtggtcggc tatcggcgcc
      841 gcgatgccgc gaccgcgagg catactgagt atctccgcgc attggtatct gccgctgacg
      901 gcggtgactg cgatgcagca gccgcggacg attcacgatt tcggagggtt tccccgggcg
      961 ctgttcgagg tgcagtatcc tgcgccggga tcaccggacc tggcgcagcg ggtgtcccaa
     1021 ctgcttgcac cggcgcaggt cgccatggac gaccgctggg gtctcgatca tggaacgtgg
     1081 tcggtccttg ctcacgcctg gcccgaagca gacattccgg tcgtgcagtt gagcattgac
     1141 gacacgagga gcgcgggctg gcactacgcg ctcgcgaagc gcctggcgcc actgcgggag
     1201 gacgggattc tcatcatggg cagcgggaac ctggtgcaca acctgcacac gtacgcgtgg
     1261 ggtgaacgcg ctgccaaacc ctacgagtgg gcggttcgct tcgaggccat ggcgcgcgag
     1321 caacttgagc gcggtgattt cgcgcccctg atcgcttacg agacgctggg gcgcgacgca
     1381 atgctggccg cgccgactcc ggaccactac ctgcccctca tctacgtgct ggcccagtac
     1441 aggccgggcg aggccgtgca cttccctgtc gaaggctttg acggcggctc catctcgatg
     1501 ctttcagtgc agttcggctg agcgctctta cccatacctt actcgccctc gttgcggatc
     1561 agtccgcggg tttgctggcg caccagctgc gcatagtagc cgccgctgcc gaggagttgt
     1621 tggtgactgc cggactcggc aattcggccg ttctgcagga cgacgatccg gtcggcgctg
     1681 accacggtac tgaggcgatg agcaatgacg aaggtcgtcc gatcggtaat gagcgtatca
     1741 agtgccgcct gtactgatgc ttccgactcc gcatccagcg atgccgtggc ctcgtcgagg
     1801 acaactatgg aaggctcctt tacgagcgcg cgggcaatcg atatccgctg tcgctcaccg
     1861 gttgagaggc gggcgccccg ttcgccgact accgttttgt aaccttcagg cagcaacatg
     1921 atgaaatcgt gcgccgacgc agcgcgcgcc gcctgctcga tttccggctg gctcgcttcc
     1981 gggcggccat acgcgatgtt ggcgctgacg gtatcgttga acagcagcgg atcctgcaac
     2041 accacgccaa tgtgacggcg cagcgaactc tgcttgagcg tgcgcaagtc ttgcccgtcg
     2101 agcagcaccg cgccggagtc ggggtcgtaa aaccgcatca gcaacgccat cagcgtcgtc
     2161 ttcccggaac cactggggcc cactacagcc agggtctcgc cggcagctac cttgagcgaa
     2221 atcccgttga gcaacggacg ctccgccggg ccgtacgaaa agtgaacgtc aatgaattcg
     2281 atgtcccccc gcaccgcctc tacgtcgtgt gcctcgggcg agtctcccag gtgctcctgc
     2341 aaatcgagaa tggagaagat ctgtcccagt gagacggtgg cgcgctgcag cgactggtat
     2401 gtggcactaa gcccctggac cgggccgaac aggccaccca cgtagcccag gaaggcaatc
     2461 agggtgccga cggtcatccg gccacgcacg actaacgccc cacctacgcc gatgacggca
     2521 atacgcgcta ttgcaacgac gaggttgctt gcagtaccga aagcagtgtc cgttgcgact
     2581 ccacgctcga cgacacgatt ggcttcgttc actcccttga gaaagcggtc cttctcggct
     2641 tcttccatgg caaagctgcg caccgtgaca agccccgcca ggacctcgtt gaagcgtgaa
     2701 taaatctgcg cccaccggtc gagcatgttc cgctctctcc gcgtctgctc gggcgcagcc
     2761 acggccgcga gaacggccgg caagggcgcg aacacgagta ccagcagtgc aagccgccat
     2821 tcgaggtcca ccattatggc tatcgccata atgagaaaca cgagcgcggg cagcacgctg
     2881 aataggagct gcgtgaccgc ggcgacaaat ccctggatgc tgcggtcgag tttggtcagg
     2941 atggcgccga cgccttcact gcgctgcatc ttgaggggca tccggtgcag gcggccgacg
     3001 gttgcttcga gcagggcgta ctgcaggcgc acgcgaatgc gccacgtgag ccagttcgag
     3061 gtgccacccg cgccatcccg gcagagcgcc agtatcccga gaaacacgag accgagccaa
     3121 acgacttcca ttccgccgtc tgcggtcagt ccgtccacga tgtacttcat gaccagcggt
     3181 tcgatggcgc tgacgacaga cacggcaagc gtcaacagca gtatggtaac gatggtgagc
     3241 cggtgcggca ccgtgaagga aagcgcgcgc cgcaactggt gcctcgtcgc aggcgctggc
     3301 ttcgatccat cgggtgtgaa gggcatatct ttacctttgc caggggaatc cggacagctc
     3361 gaaacaaaag caccgggaga cagggtggct ggaacgccag tggcgggcga ggctcctacg
     3421 ggcgactcga cccgtccata tttccaccag ccaaacagca attcaaccga acactgccgg
     3481 cgggccggac gagcacccac aggtacccaa ccggattgca tcaacgtatc atcgccagat
     3541 cgtgtgccct tacagtttta cgacgcttga ggtcgattag tgcctgctga agaattcgat
     3601 gcgattgtcg ttggttccgg aatctcaggt ggctgggccg ccaaggaact taccgagaaa
     3661 ggtctgcgag tactgcttct cgaacgtggc aagaacgtcg aacacatcaa ggattacgtc
     3721 aacgcgcgca aggcgccgtg ggagtttccg catcgcggcg gccgtaccca tgcgatggag
     3781 gaagcttatc ctgttctcaa acgggactat ccgctcaacg agaagaatct ggactggtgg
     3841 gcgtccgatg cggattctcc gtacaccgag gtaaaacgtt tcgactggtt tcgcggctat
     3901 cacgtcggcg gacgctcgtt gatgtgggga cgccagagct accggtggag cgactacgat
     3961 ttcgaagcga acgctcgcga tggcgtggct atcgactggc caatccgcta cgcggacctc
     4021 gcgccatggt acgaccacgt ggagcggcac gccggtatat ccggctcgca agaacagatg
     4081 ccgcaacttc cggacgggca gttccagccg gcgatgccgt tgaactgcgg cgaagagctg
     4141 gtcgcgggca ggctgaaggg tttattcgat ggccgtcgcc ggatcatccc cgggcgggta
     4201 gccaatctca ccgaggcgct ccccgggcgg gcgccatgcc agtatcgaaa tgcctgctgg
     4261 ctgggttgtc catacggggg ctacttcagc acccagtcct cgacgttgcc ggccgccgtc
     4321 gctacagaac ggctcacgct caagcctttc gccatagtca cggaagtcct ctatgaccgc
     4381 gaccgcaggc gcgcaagcgg cgtgcgtgtg gtagacgccg tgacggagca cagcaccgaa
     4441 tacaaggcac cggtcatatt cctttgtgcc tcgacgctca acagcacgtg gatcctgatg
     4501 cgttccgcga ccgatgtgtg gccggacggg ttgggaagca gctcaggcga actgggacac
     4561 aacctgatgg atcaccactt ccggctgggc gcgtcgggca agctcgcgga agcctcgcta
     4621 gcggggcgct actactacgg gcgcaggccg aacggttttt atatcccgcg ttaccgcaac
     4681 ctgttcggcg acaagcggcc atacctgcgg ggatttggtt accagggttc cgcaagtcgc
     4741 aacgactggc aacgtgcggt aggcgaactg ggtatcggag ccgaattcaa ggatgccatg
     4801 tcacagccgg gacagtggga aataggggcg acggctttcg gcgagatgct gccgaaccac
     4861 gacaacaggg cgtccatcga ctcgagcagg acggacaagt gggggctgcc ggtgctgaag
     4921 atcgactgcg cgacgggcga gaacgagcgc ctgatgggca tcgacatgaa aaacgacatg
     4981 gccgagatgc tcgaggcggc tggtgcgcgc gacgtggaac cctacgacag gggttacttt
     5041 cccggcatgg gtatccatga aatgggtacc gcccgtatgg gccgcgatcc gcgcaggtct
     5101 gtgttgaacg aatggaacca ggtgtgggac gcgccgaacg tgttcgtcac ggacggcgcg
     5161 tgcatgacgt cgtccgcctg ccagaaccct tcgctgactt acatggccct gacggcgcga
     5221 gccgcagact atgccgtgaa agagctgaag cgccgcaacc tctgacggta ggggaacaac
     5281 catgtcagcc gattcccagc gtcccgacct cattacacgc cgcgaagcga tcctgcgcgt
     5341 cagcgcattg ctcggaggag cagcgctggt cggacaaacc gcgatgctcg ccggctgcgc
     5401 acgcgagggc gtccagtcgc aggccttctc cgccgacgat atggctttac tcgacgagat
     5461 cgcggagacg attcttccgg aaaccggtac gcccggtgcc aaggcggcgg gcgtcgggcc
     5521 tttcatcgcg atgatggtcg tcgataccta tgacgagcgg gagcaggagg tattccgcgc
     5581 cggcctgcaa gcgctggaag atgaatgccg cgccatgcac ggcatgtcct tcctggcggc
     5641 cacgcccggg caacgaaccg ttctgctgcg gaagctcgat gccgaacagc tgcagttcat
     5701 gcagacagca ggaaagcacg agccggcgca ctacttccgt atgctgaaag agctaacatt
     5761 gctcggctat ttcacgtcgg agatcggata cacacaggca atgcgttaca tcgaaacacc
     5821 cggacgttat gacccctgtg tgccctggga gccgggcgaa aaaacctggg cggtccatgc
     5881 ctgaggcacc cgcctccgca agcctgtttc gggagggaag ttttcgccgc ctggtcataa
     5941 agatcgggtc gtcactgctg accgatggga gcggagcggt caaccggcca tggctgtcgg
     6001 gtatcgcgga agatattact gcgcttcgtg ccagggattg cggggtcctg gtcgtaacgt
     6061 cgggtgcgat tgccatcggc tgccgcgcca tcggcatcaa caggcgacga gcgcggttgg
     6121 agatgctgca ggccgcagct gcctgcggcc aggtgcagct catgcaagcc taccaggaag
     6181 cactgggccg ccatggcatc gcggtcgcgc aggtgctgct cacgctggat gatacggaac
     6241 accgacgccg gttcctgaat gccaggggca ccctggaaaa gctgcttgaa catgatgtcg
     6301 tgccggtgat caacgagaac gacacggtcg caacggacga gatccgctac ggcgacaacg
     6361 accggctcgc cgcgagagtt gctcaaatgg tgatggcaga cgggttgctg ctgttgtcgg
     6421 acatcgatgg cctttacacc agcgatccgc gggaaaaccc cgatgccgaa cacgtagccg
     6481 gcatcggcga tatctcggat gacgtcatcg ccatggcggg cggaccgcgc tccgctgacg
     6541 gcagcggtgg catggcgacg aagctccagg ccgcgcagat cgcaacgcac gccggatgca
     6601 gaaccattat cgcgaatggt aacggtccgc ggccggtctt ggcgctggcc agtggcgccc
     6661 gctgcacgat gttccgcgca cagggtacgc ctgcctcggt gcggcagcag tggctggctg
     6721 gtgcgctgag aacccgcggt gccttgcacc tcgatgccgg cgccgtcgcg gcgttacagg
     6781 agggcagcag cctgcttccg gtaggcgtca ccggggtgac cggcgacttc gcgaggggcg
     6841 atcctgtcgc gttgctgaat ggcgacggca gggaaatcgg ccggggcctg atcgcctacg
     6901 agagcagcga agcggcaagg atcatcggtt gccacagcga tgagcttgaa gcccggctcg
     6961 ggtatcgcgg ccgctctgtc atgattcacc gcaacgacct tgtctttttc ggcaggacga
     7021 agtaaccggc atggaacagc agcaggaccg gggattatga acatcgggcc gatgatgtgt
     7081 gagatgggac gtgccgccaa actcgcgtcg caagcgctgg gtgcggcggg tacggctgcg
     7141 cgaaacgttg cccttcagga atgcgcgacc gctattcgta atggtgctgt tgcgattctc
     7201 gatgcgaacc ggcaggacct tgaacgcgcc cggaagctcg agctcggtag tgcgatgacc
     7261 gaccgcctgg ccctcgacga atcacgcgtc gaagcaatgg cgaaaggcct ggaggccatc
     7321 gccgcactgc cggacccgct cggtcgcgtc atcgccggct ggcagcggcc gaacggactg
     7381 aagatcgagc gcgtctcggt cccgcttggc gtcatcggca tcgtttatga aagccgcccg
     7441 aacgtcacgg ctgacgccgc gggactctgc atcaagtcgg gcaacgcagt gattcttcgg
     7501 ggtggttccg agagctttct ctccagctcc gcgatctacg cgtgcctgca ggaagggctg
     7561 aagaccgccg gattgccgct cgagtccgtg cagatggttc caacgacgga tcgcgccgcg
     7621 gtcggctaca tgctgtcgtc catgagagag tacatcgacg tgctggttcc ccgcggcggc
     7681 aaacgcctca ttgcgcgggt gcaggaagat gcgcgggttc cggtaatcgg gcacctggaa
     7741 gggatctgcc acgtctacct tcatgcgtcg gcggaccagg caatggcgca ggacatcgtg
     7801 ctgaattcca agatgcggcg caccgggatc tgcggtgccg ccgagacgct gctggtcgat
     7861 cgcggtgccg ccgaacggat gttgcctccc atcatcgccg cgttatgcga ggccggttgc
     7921 gaagtgcgcg gcgatggcat ggtgcagtcg atctccggca gtgtattgcc ggccacggaa
     7981 tccgactgga gtaccgagta cctcgacgcg attatttccg tgcgcaccgt cgaggatgtc
     8041 gatgatgcca tcaggcacat ccggcgctac ggttccggtc acaccgaagc tatcgtcgct
     8101 gaagactcaa cggccgcaga gcacttcctg cgcggtatcg acagcgcgat cctgttgcac
     8161 aacgcctcga cgcaattcgc cgatggcggc gaattcggca tgggcgcgga aatcggcatc
     8221 gcaaccggaa agattcacgc ccgcgggccg gtgggcgccg aacaactgac gagctacaag
     8281 tacgttgtac gtggcgccgg gcaggtacgg ccctgagcat aaaggggcgg attgtctttt
     8341 gtattcggga tgcgccttga accgcagcaa aaacagatct gtccccttca tgctcatcgc
     8401 ggccgcactc accggaatag ctgcctgcaa cggaacacct cccgactcgg tggagggcac
     8461 aacgaaatcg gggcctggct ggaagacagt caacgatggc gtagtcgtta gcttcgacaa
     8521 cgcagagttc cgccgggtac gcctgcaggt cgtagatgac ggcatcatcc gggttaccgc
     8581 cacaccgcag gaggacttca gcaacctgcc cgatactctc atgatcgtcg caggcgcgcc
     8641 ggatgccggg tttcgcgcgg agcagcgcgg gaacgaagta gtggttcaga ccggagccct
     8701 ggaggcgcgg gtatcgctgg caacgggcac cgtgcgcttc acggattctg acggcaacga
     8761 gctgcttgcc gaagcggaac ggagccttgc accggttact gcggatccgg gcgcaaccga
     8821 tgatgattcc ttcttcgtcc gccagcagtt tcaacgggcc acggacgagg acgaagggat
     8881 atacgggttc ggtcagcagc aggacggccg tgtcaattat gcgggcagga acgtcgagct
     8941 gacgacgcac aatatcgaga ttgctattcc gttttttgcg tccagccgcg gctatggcgt
     9001 gctctggaac aatacttcca tcacccgctt cggagaccct gagccgccac gtcctctgtc
     9061 agcgggcttc cggttgttcg atgcgaacgg agagcccggc ggcttgaccg ccaggtacta
     9121 cgcaggtggc gaattgaagc tcgaacaggt ggagccagac ctcaattacc agttcctgtc
     9181 acacgcgaac gtgcgcgaaa taccgttacc ggaggaggtg cgggattcgg atgacgtgcg
     9241 catcgagtgg gagggcagct tcgttccgtc gaacgatgga atccacgagc tgaag
//
LOCUS       DASUOM010000589         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_370466,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000589 DASUOM010000000
VERSION     DASUOM010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370466"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFY27_12295"
     CDS             complement(<1..718)
                     /locus_tag="VFY27_12295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5766342.1"
                     /translation="MRIAQVSPLIESVPPLLYGGTERVVSSLTEQLVAVGHDVTLFAS
                     ADSQTNAHLVACAPSALRLEPRSIDPWPHYMMMLEQIRRRAKEFDLIHFHIDYLHFPL
                     MRELAIPHLTTRHGRQDLWDLAPLFAAYPDMPLVSISDSQRRAQPEGNWVGTVYHGLP
                     RQRFRWSRSGGDYLVFLGRVSPEKRVDRAIEIAERSGRELVIAAKVDKADHEYFETCI
                     KPVLNRPFVRFVGEVGDAGKA"
     gene            complement(1169..>3325)
                     /locus_tag="VFY27_12300"
     CDS             complement(1169..>3325)
                     /locus_tag="VFY27_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766343.1"
                     /translation="KVTSSLIATTITLPLLMVSQLASSQSEQVLSEVGAMGKLANGRQ
                     GVVIPDFSNETSADYSEWVALATGRDFKSFAISFRGTALVLDGTMIREFNSAGQELMN
                     AGFPVDCKKPFPITKSSGQPGTDSLSTCTAFTPLNDGTIRIAGQSKGKSLVMVGYDPE
                     SGQMRLKASGTPPSISDMDGDEYADSDDRKGAGYWAIGDKKKIIFFPLNNENNFEVVA
                     TINGVDLDAITPFGPHRLIVALTTGELREVNTASGVSTSKAFLPTGADCQLGRRDPQK
                     FSLRGDPSGVLFVGNMGCREITLFDSNLQQVAASDSFSGNPFELNNPSPFFVSGLDWQ
                     SGQGGDFGDCDGLTETDGCKFGIEPRQAVMWEVENVGSDTSYRMFQFVNLDDCRWSGN
                     RPCPILNCPSADGKGAACPVTDKKEQVLDLAQVLIRADQSGVFEEMAFGDGPVPEMAL
                     PGYMRGEECFPSSGDSNVCVPNNYRFHSFFAVTDAVFTGNFFTDYKIDEFRVGSDDPC
                     LIPEPESTIEEVNETANLIVYNTDSFGTVDRGGVQGRRGGVIVNDACNGRAGGYKWSS
                     QTIGLELYDDDNPEAYIDQANRMMGELIQAKEELLCAPFPDDSSLGPLLLGSDCNSIG
                     GELSQMESKLATCFDSLYAPQSGNSAENCSAFFTKVQNLNNVLDASAWPVPNTTNLDV
                     LRPNYEGEFRARLATLVFFIQSYVLNSVPPGGIILP"
BASE COUNT          678 a          965 c          972 g          710 t
ORIGIN      
        1 ccgccttgcc ggcgtcgccc acttccccga cgaagcgcac gaaggggcga ttcagcaccg
       61 gcttgatgca ggtctcgaag tattcatggt ccgccttgtc cactttcgcg gcaatgacga
      121 gctcacgtcc cgaccgctcg gcaatctcga ttgcgcggtc gacccgtttc tccggagata
      181 cccggccgag aaacacgaga tagtccccgc cgctacggga ccacctgaat ctctggcgtg
      241 gcaggccgtg atagaccgta ccaacccagt taccctcagg ctgtgcccgg cgctggctgt
      301 cggaaatgga taccagcggc atgtccgggt aggcagcaaa cagcggcgcc aggtcccaca
      361 ggtcctggcg gccatggcgc gtcgtcaggt gcggaattgc cagttcgcgc atcagcggga
      421 agtgaaggta gtcgatgtgg aagtgaataa ggtcgaattc cttcgcccgc cggcggattt
      481 gctccagcat catcatgtaa tgcggccaag ggtcaatgga ccgcggctcc aggcgcagcg
      541 ccgagggcgc gcaggcaacc agatgagcat tcgtctgcga atccgcggaa gcaaacagcg
      601 taacgtcatg gccgaccgcc accagctgtt ccgtaagact tgaaacgacg cgctccgtgc
      661 cgccgtataa caggggggga accgactcaa tgagcgggga aacctgcgcg atcctcatct
      721 ttctcgagtt gcctctaatc ggatgacagg gtccccagcc ccgccaggct aatcaacagc
      781 tgctactctt atatgtatac caccgaaagt tttcaacccc gggaagtcgc cgcattgaga
      841 cagtgcttgg aggcctcgta gcacgcgtct cagcacgtaa ttcttacaac ttacctgacg
      901 tatcggagcc tgcgtttcgc atgcctcgtt gcaaatactg ccgctggcta tgcaaattat
      961 ctacccccgc ccgggacagc gaggtcagat gtgatgacgg taaatgtgcg gcaggctggg
     1021 agttcctgca gagtggaaca gcggatatgc gcacgatgcg cgtgggattg atatgcgttt
     1081 cctacgctaa tgatccggcg atatagccga actgctgcca ggaaagaacg cggcggcccc
     1141 ggtttgaggc cgccgccagg attgtcgctt aaggcaggat tatgccgccc ggcggcacgc
     1201 tgttcagaac gtagctttgt atgaagaaaa ccagcgttgc caggcgcgcg cggaattcac
     1261 cttcgtagtt aggcctcaaa acgtcaagat tggtggtgtt gggaacgggc caggcagagg
     1321 catccagtac gttattgaga ttctgcacct tggtgaagaa agcgctgcag ttctcggcgc
     1381 tgttgccgct ctggggtgcg tacaacgaat cgaaacatgt cgcgagcttc gattccatct
     1441 ggctcaattc accgcctatc gaattgcagt ccgagccgag taacagtggc ccgaggctgc
     1501 tgtcgtcggg gaacggggca cacagcagtt cctctttcgc ctggatcagt tcgcccatca
     1561 tacgattggc ctggtcgata taagcctccg ggttgtcgtc gtcatagagc tcgagtccga
     1621 tcgtttgcga agaccatttg tagccgccgg cgcggccgtt gcaggcgtcg ttgacaatca
     1681 caccaccccg ccttccctgc acaccaccgc ggtcgacggt gccgaagctg tcggtgttgt
     1741 agacgatcag gttggctgtc tcgttgactt cttcaatggt gctctcaggt tccgggatca
     1801 ggcatgggtc gtcactgcca acacgaaact cgtcgatctt gtagtccgtg aaaaaattgc
     1861 cggtaaagac agcgtctgtc acagcgaaaa aggaatgaaa gcggtaattg ttcggtacgc
     1921 atacgtttga atcgccactg cttgggaagc actcttcgcc gcgcatgtat ccaggtagcg
     1981 ccatttcagg taccgggccg tcgccgaatg ccatttcctc gaagacgccg ctctggtcgg
     2041 ccctgatcaa tacctgtgcg aggtcgagaa cctgttcttt cttgtcggtg accggacagg
     2101 cggctccctt accatcggcc gacgggcagt tgaggatggg gcacggccgg ttgcccgacc
     2161 agcgacaatc gtccaggttt acgaactgga acatgcgata cgaggtatcg ctgcccacgt
     2221 tctctacttc ccacatgacg gcctggcggg gctcgatgcc gaacttgcag ccgtcggtct
     2281 cggtcagtcc gtcgcagtct ccgaaatccc ccccctggcc gctttgccag tcgaggcctg
     2341 agacgaaaaa cggggaagga ttgttgagct cgaacgggtt tccgctgaag ctgtcgctgg
     2401 cggctacctg ctgcaggttt gagtcgaaca gggtaatttc gcggcaaccc atgttaccga
     2461 cgaagagcac gcccgagggg tcaccacgga ggctgaactt ctggggatcc cttcttccca
     2521 attggcagtc cgctccggtt ggcaaaaaag ccttggaggt ggaaactccg ctggcagtgt
     2581 tcacctctcg caattcaccg gtagtcagcg caacgatcag ccggtgcggt ccaaaggggg
     2641 tgatagcatc gagatcaacg ccgttgatcg tagccacgac ttcaaaattg ttttcattgt
     2701 tcagcggaaa aaagatgatt ttctttttat cgccgattgc ccagtaaccg gcacccttgc
     2761 ggtcgtcaga atcggcatac tcgtcgccgt ccatatctga gatggaggga ggcgtgccgc
     2821 ttgctttgag tctcatctgg ccgctttcgg gatcgtatcc gaccatgacc agcgacttgc
     2881 cctttgactg cccggcgatg cggatcgtgc cgtcgttgag cggcgtgaag gcggtgcagg
     2941 tcgacagaga atccgtgccg ggctgtccac tggactttgt tatcggaaac ggttttttac
     3001 aatctaccgg gaatccggca ttcatcagct cctgtcctgc agagttgaac tcgcggatca
     3061 tggtcccatc gagcaccagt gccgtgcccc tgaacgaaat cgcgaagctc ttgaagtcgc
     3121 gcccggtggc caacgcgacc cactccgaat aatccgcgct ggtttcattg ctgaagtcgg
     3181 gtataacgac gccctgcctg ccgttcgcaa gcttgcccat tgcacccacc tcgctgagca
     3241 cctgttcgct ctggctcgac gcgagctgcg aaaccatgag caatgggaga gtgatcgttg
     3301 ttgcgataag ggaactggtg acctt
//
LOCUS       DASUOM010000590         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1387416,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000590 DASUOM010000000
VERSION     DASUOM010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387416"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1018
                     /gene="galE"
                     /locus_tag="VFY27_12305"
     CDS             8..1018
                     /gene="galE"
                     /locus_tag="VFY27_12305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5766344.1"
                     /translation="MGDKTILVTGGAGYIGSHVVRQLGAAGESVLTLDNLTTGFREAV
                     TAGELVVGDTGDAALLENLFVDHDIDTVMHFAAHTIVPESVSDPLKYYRNNTASSRTL
                     LECAANHHVKHIVFSSTAAVYGIPAGGRAAEDSPTQPINPYGTSKLMTEWMLRDLAFS
                     GGPSYVALRYFNVAGCETSGRIGQSTPKATLLVKVACEAAVGRRSHVSVFGTDYPTPD
                     GTGLRDYIHVEDLATAHVDALAYLRKGGESTTLNCGYGHGYSVREVLSAVEKANGKPL
                     KIVEEARRAGDPPELVAVADRIRKVLGWKPKLDNLDTIVGTSLAWERKIAAGDPAAYW
                     AA"
     gene            1092..>2479
                     /locus_tag="VFY27_12310"
     CDS             1092..>2479
                     /locus_tag="VFY27_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5766345.1"
                     /translation="MSLPRPRSLNGLILVGFGLVALPLLIAVVWALVSLDRLAEQSEQ
                     LIVSGVTAAENNRALAQQVASFERLARQYLVLRTEDSLSLTRQDVASIEEITALILPF
                     AAMADATELARSIPARARSLVDALSQDIVSENDGQEAIAEFATLRQDVTRLSSIISTH
                     IELELSALQASIRRAQRVSAWQTAALVPGTVVVVLFFTLLVAKPIRQIDRAINQLGQS
                     GFSKPIEVKGPTDLEKLGRQLEWLRVRLLELAQEKNRFLRHMSHELKTPLANIREGTE
                     LLLDGTVGELDASQREVTDILRQNGLKLQRLIENLLSFSAWQTKNEVLTLSQFSLRPL
                     IVSVAKAQRLALKSAQIQLRLQVEDCVITADRDKIRTVLDNLLSNAVKFTPRGGRVTI
                     RSVAKPGSCEIEFADTGPGIPDDEGPRIFDAFFQGKQKQGGHIAGTGIGLSVVQECIQ
                     AHDGVIELVDSD"
BASE COUNT          497 a          770 c          780 g          432 t
ORIGIN      
        1 agaaacaatg ggtgacaaga cgatcctggt caccggcggt gcaggctaca tcggcagcca
       61 cgttgtgcgg caactgggcg ccgctggcga atccgtgctg acgctcgaca acctgacgac
      121 cggcttccgc gaagccgtga cggccggcga gctagtggtc ggcgacacgg gtgatgcggc
      181 gctgctcgag aacctgttcg tcgaccatga catcgacacg gtcatgcatt tcgcggcgca
      241 caccatcgta cccgagtcgg tctcggaccc gttgaaatac taccgcaaca acaccgccag
      301 cagccgtacc ctgctggaat gcgcggcaaa ccaccatgtg aagcatattg tgttctcctc
      361 gacggcggcg gtgtacggca tcccggcggg cggtcgcgcc gccgaggatt cacccacgca
      421 accgatcaat ccttacggca cgtccaagct gatgacggaa tggatgctgc gtgacctcgc
      481 gttctcggga ggcccgtctt atgtcgctct ccgttacttc aacgtcgccg gctgcgagac
      541 cagcggacga atcgggcaat ccacgccgaa ggcaacgctc ctggtgaagg tcgcatgcga
      601 agccgcggtg ggacggcgca gccatgtctc cgtcttcggt acggactatc ccactcccga
      661 cggaaccggc ttgcgcgact acatacacgt cgaagacctc gcgaccgcgc atgtggacgc
      721 attggcctat ttgcgcaagg gaggcgagtc aacaaccctc aattgcggct atgggcacgg
      781 ctacagcgtg cgcgaagtgt tgagcgcggt cgagaaggca aacggcaagc ccctgaagat
      841 tgtcgaagaa gcccgccgtg ccggcgaccc gcccgagctc gtcgcagtgg cggatcgaat
      901 ccgcaaggtg cttggatgga aaccgaagct cgacaacctg gacacgatcg tcggtaccag
      961 cctcgcctgg gagcgcaaga tcgccgccgg tgatccggcc gcttactggg ccgcctgaac
     1021 gctctttgaa cgcacccgaa acagagtcaa atgacccgcc gttcggctgg atttcgggct
     1081 aaacttccgc aatgtcgctg ccccgccccc gctcgctcaa tggactgata ctcgtaggat
     1141 tcggcctcgt tgcattgccg ctcctgattg cggtggtgtg ggcgctggta agcctggacc
     1201 ggcttgcgga gcagagcgaa cagctcatcg tcagtggcgt gacggcggcg gagaacaacc
     1261 gcgcgctggc gcaacaggtg gcatcgttcg aacgtctcgc ccgccagtac ctcgtactgc
     1321 ggacggagga cagcctgtcg ctgacgcggc aggacgtggc gtccatcgag gaaatcaccg
     1381 ccctcattct gccgttcgca gcgatggccg atgccacgga gctcgcccgg tcgatcccgg
     1441 cgcgcgcacg aagtctcgtg gatgccctgt cgcaggacat cgtcagcgaa aacgacgggc
     1501 aggaagccat tgccgagttc gccacgctgc gacaggacgt tacgcggctg tcgtcgatca
     1561 tcagtaccca catcgaactc gaactgagcg cactgcaggc aagcatccgc cgggcccagc
     1621 gggtgtccgc gtggcagacg gctgccctcg tgcctggcac cgtcgtcgtc gtgctgttct
     1681 tcaccctgct ggtggcgaag ccgatccggc agatcgatcg cgccatcaac cagctgggcc
     1741 aaagcggctt ttcgaaaccg atcgaggtca agggtccgac cgacctcgag aagctggggc
     1801 gccagctcga gtggctgcgc gtgcgcctgc tggagctggc ccaggaaaag aaccgcttcc
     1861 tgcggcacat gtcgcacgaa ctcaagacgc cgcttgcgaa catccgcgaa ggaaccgaat
     1921 tgctgctcga cggaacggtc ggcgagctgg atgcgtcgca gcgggaagtc accgacatcc
     1981 tgcggcagaa cggattgaaa ctgcagcgcc tgatcgagaa cctgttgagc ttcagtgcgt
     2041 ggcagacgaa gaacgaagtc ctgaccctgt cgcagttttc attgcgcccg ctgatcgtgt
     2101 ccgtggccaa agcgcagcgc ctggcgttga aaagcgcgca gattcagctg cgactccagg
     2161 tagaggattg cgtcatcacc gcggatcgcg acaaaattcg aactgttctg gacaacctgc
     2221 tgtcaaacgc cgtcaagttc accccgaggg gggggcgcgt taccatccgt tctgttgcca
     2281 agcccggaag ctgcgaaatc gaattcgcgg acacggggcc gggcatcccg gatgatgaag
     2341 gtccgcgaat ttttgacgcg ttctttcagg gcaagcagaa acaggggggg cacatcgccg
     2401 gcaccggcat tggtctttcg gttgtgcagg aatgcataca ggcacacgac ggtgtcatag
     2461 aacttgttga ttcggatga
//
LOCUS       DASUOM010000591         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1771014,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000591 DASUOM010000000
VERSION     DASUOM010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771014"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1398)
                     /locus_tag="VFY27_12315"
     CDS             complement(97..1398)
                     /locus_tag="VFY27_12315"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5766346.1"
                     /translation="MGNNVVVIGTQWGDEGKGKIVDLLTDRAAAVVRFQGGHNAGHTL
                     VIGGKKTVLHLIPSGILRDGVRCLIGNGVVVSLTALIKEAEALIASGVPVFERLKLSP
                     GCPLILPSHVALDIAREKSRGAAAIGTTGRGIGPAYEDKVSRRALRVADIFAREKFAA
                     KLGEVLDFHNFLLRNYFRAEPVSFAQTLDAALQEAEVIAPIVADVTQLLDELSRAGES
                     ILFEGAQGAFLDIDHGTYPFVTASNTVAAAASTGTGVGPKELGYILGIVKAYTTRVGA
                     GPFPTELFDDQGEHLATVGAEFGATTGRPRRCGWFDAVALRGAIVNSSVSGLCVTKLD
                     VLDGLEKIRICVGYELDGVRVASLASVIGRLAECQPIYEDMPGWTQSTVGVTRYAALP
                     TNAQQYLERIEELAGVPLDIISTGPDREQTIVKRHPFDTFA"
     gene            complement(1444..1632)
                     /locus_tag="VFY27_12320"
     CDS             complement(1444..1632)
                     /locus_tag="VFY27_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX5766347.1"
                     /translation="MWQDILTAFALYLIIEGMIPFIGPDRFRRTVEQISRLNDNNLRT
                     MGFIAMASGLLLLFIVRA"
     gene            complement(1693..>2283)
                     /gene="hflC"
                     /locus_tag="VFY27_12325"
     CDS             complement(1693..>2283)
                     /gene="hflC"
                     /locus_tag="VFY27_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5766348.1"
                     /translation="VDAVNFYTSTGGSLTLANDRLSEIVRNAIVTELGKRTVQQAISV
                     ERVELMRDMLDSASETAEGLGVDLVDVRVKQVEFPDDVRNSVYQQMREERARVAAQRR
                     AEGREIAEERKATADRDQTVILAEANRQSQIIRGEGDATAAQIYAAAYNRDPEFYTFY
                     RSINAYRESVGKPGDILVIEPDNEFFRYLNESGGAD"
BASE COUNT          422 a          726 c          676 g          459 t
ORIGIN      
        1 caaccaccgt cgtgaccgta gcaaaagcag gcagcggcgg tggtggtggc ggcagcgtgt
       61 ctgtgctgtt ctttctcttc ggtggcgcga cgctcttcag gcgaatgtat cgaaaggatg
      121 tcgcttgaca attgtctgtt cgcgatcggg gccggtcgaa atgatgtcca gcggcacgcc
      181 cgcaagttct tcgatacgct cgagatattg ttgcgcgttt gtcggcaggg ccgcatagcg
      241 ggtgacgccg actgtggact gggtccagcc cggcatgtct tcatagatcg gctggcattc
      301 agccaggcgg ccaatgacgc tagcgaggct cgcgacccgt acaccgtcca gctcataacc
      361 gacgcaaatc cgtatttttt cgagtccgtc cagcacatcg agcttggtca cgcacagtcc
      421 cgaaacgctg ctgttgacga tcgccccccg tagcgcgacg gcgtcgaacc agccgcaacg
      481 ccgcggtcgc ccggtggttg ctccgaattc ggcaccgaca gtcgccaggt gctcgccctg
      541 gtcgtcaaac agctcggtcg ggaacggtcc tgcgccgacg cgggttgtgt atgccttcac
      601 gattcccagt atgtaaccga gctccttcgg gccgaccccg gtgccggtgc ttgctgccgc
      661 cgcgacggtg ttggatgccg tgacgaaggg ataggtgccg tggtcgatgt cgagaaacgc
      721 tccctgcgcg ccctcgaaaa ggatgctctc ccctgcccgc gaaagttcat cgaggagctg
      781 cgtgacatcc gcgacgatcg gcgcgattac ttcagcctcc tgcaacgcgg cgtccagcgt
      841 ctgggcgaaa cttacgggtt ccgcacggaa ataattgcgc agcaggaaat tgtgaaagtc
      901 cagtacctcg ccaagcttgg ccgcgaattt ctcgcgcgca aaaatgtcgg ccacccgtag
      961 cgcccgccgc gaaaccttgt cctcgtaagc cgggccgata ccgcggccgg tggtgcctat
     1021 cgcggcggcg cccctcgatt tttcacgcgc gatgtcaagc gctacgtggg acggcaaaat
     1081 gagtggacat ccgggactca gcttcaggcg ttcgaacacc ggcacgccgc tggcaatcag
     1141 cgcttcggct tccttgatca gcgcggtcag cgacaccacc acgccgttgc cgatgaggca
     1201 gcggacgccg tcgcgcagga taccggatgg aatcaggtga agaacggtct tcttcccgcc
     1261 gatcacaagc gtgtgcccgg cgttgtgccc gccctggaag cgaaccacgg cagctgcacg
     1321 gtccgtcagc aggtcgacaa tttttccctt gccctcatcg ccccattgcg tgccgatgac
     1381 aacgacgttg tttcccatct ccggttcctt gaccgctcgc ccgaggctag ttacaggcca
     1441 ctactaagcc cttacgatga ataaaagaag aaggcccgac gccatcgcta taaagcccat
     1501 cgttcgcagg ttattgtcat tgagcctgga gatctgctcg acggtccggc gaaaccggtc
     1561 ggggccgatg aacggaatca tgccctcgat gatcaggtac agcgcaaatg ccgtgagtat
     1621 atcctgccac atgaggcact caggctcgca gctgcaatcg ccgcaaggcg cgcggtgcag
     1681 tccatagctg cactagtcag cgccgccgga ttcgttgaga tagcggaaga actcgttgtc
     1741 cggttcgatt accaggatgt cgcccggctt cccgaccgac tcccggtagg cattgatgct
     1801 gcggtagaac gtgtagaact cgggatccct gttataggcg gccgcgtata tttgggccgc
     1861 cgtggcatct ccttcaccac gtatgatctg gctctggcgg ttggcttcgg caaggataac
     1921 ggtctggtcg cgatccgcgg tcgcctttcg ctcttccgcg atttcacgac cctctgcacg
     1981 gcgctgcgcg gcgacccgcg ctcgctcttc ccgcatctgc tggtacaccg aattgcgaac
     2041 gtcatcggga aactcgacct gcttcacgcg tacgtctacc aggtcgacac cgagcccctc
     2101 agccgtctcc gacgcggaat ccagcatgtc gcgcatcagc tccacgcgct ccaccgagat
     2161 tgcctgctgc acggtccgct tgccgagttc ggtcacgatc gcgttacgca cgatttcgga
     2221 caggcgatcg ttggcgagcg tcaacgatcc acctgtcgag gtatagaagt tcacggcatc
     2281 gac
//
LOCUS       DASUOM010000592         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_616619,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000592 DASUOM010000000
VERSION     DASUOM010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616619"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..994
                     /locus_tag="VFY27_12330"
     CDS             23..994
                     /locus_tag="VFY27_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX5766349.1"
                     /translation="MTAVRDFVHHHYRHFNAAALVDAADGYVCHLDQGGVMFMTLAGA
                     MSTAELGLSLAEMIRRDKVHAICCTGANLEEDVFNLVAHDHYVRMPHYRQLTAEDEEA
                     LLARHLNRVTDTCIPEEEAIRRIEHEILDIWLDADRKGERYFPHEFLYEVLRSGKLDR
                     HHQIDPRDSWLVAASEKDLPIIVPGWEDSTLGNIYASHCITGEISNVHTVRSGIEYMI
                     YLADWYERTAGHSSVGFFQIGGGIAGDFPICVVPMLQQDLRKEDTPRWGYFCQISDST
                     TSYGSYSGAVPNEKITWGKLSADTPKYIIESDATIVAPLIFACVLDE"
     gene            999..>2345
                     /gene="speA"
                     /locus_tag="VFY27_12335"
     CDS             999..>2345
                     /gene="speA"
                     /locus_tag="VFY27_12335"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX5766350.1"
                     /translation="MSETAAPAESWSREDASELYRMPAWSDGFFRINERGHITVHPFE
                     DSDLCIDIMDVVEEAERRNLTFPLLLRFQDVLRARVQRLNAAFSSAIEEAAYQNVYRS
                     VYPIKVNQLHEVVEEVLDAGKPFELGLECGSKAELVAALPHLSQDQTLLICNGVKDRT
                     MLSLILAAQGLGKNVIPVMEKFAEFEQLMALVDEQETETRFGVRIRLRTAGSGRWADS
                     GGYRSKFGVSLPELVELVDQLERTNRLDSFVLLHFHLGSQIADIQTLKQAVKEMAQIY
                     AELKARGLPIRYFDVGGGLGVNYSGGYDEGGINYSLQEYANAVVYSVMEVCNAKSQPH
                     PVLISESGRAITAHHSVLVVETLGAFRKDRVPGDFKVPADAAPMVRDLGDILQRLEAR
                     PARKMSVSQLLEAYHDIVEIHREAATLFSVGYLPLQQNALVERLYWSCCAAILKRLR"
BASE COUNT          465 a          712 c          729 g          439 t
ORIGIN      
        1 ccacactact ggagattcgc ctatgactgc ggttcgcgat tttgttcatc atcattaccg
       61 tcatttcaac gcggcggcgc ttgtcgatgc ggccgatggt tatgtgtgcc acctcgacca
      121 gggcggcgtg atgttcatga cgctcgccgg tgcgatgagc accgcagagc tcgggctctc
      181 gctcgcggag atgattcgcc gcgacaaggt ccacgcgatc tgctgtaccg gcgcgaacct
      241 ggaggaagac gtgttcaacc tggttgcgca cgaccattac gtccgcatgc cgcactaccg
      301 gcaattgacg gccgaagacg aagaagcgct gctggcccgc cacctgaacc gcgtcaccga
      361 cacctgcatc ccggaagaag aggcaatccg ccgtatagag cacgaaatac tcgatatctg
      421 gctggacgcg gacaggaagg gcgagcgtta ctttccgcac gagttcttat acgaggtatt
      481 gcgctccggc aagctcgacc ggcaccacca gatcgatcct cgggacagct ggctggtcgc
      541 cgccagtgaa aaggacctgc cgatcatcgt ccccggctgg gaggattcga cgctcgggaa
      601 catctacgcc tcgcattgca taacgggcga aatatcgaac gtgcacaccg tgcgaagcgg
      661 catcgagtac atgatttacc tcgcggactg gtacgagaga actgcgggcc actcgtctgt
      721 cggcttcttc cagatcggcg gcggcatcgc cggcgacttc ccgatctgcg tcgtacccat
      781 gctgcagcag gacctgcgca aggaagacac gccgcgctgg ggatacttct gccagatcag
      841 cgattcgacg acgagctacg ggtcctactc cggcgccgtg ccgaacgaga agatcacctg
      901 gggcaaactc tccgcggata ccccgaaata catcatcgag tccgacgcaa ccatcgttgc
      961 gcctctgatt ttcgcgtgcg ttctcgatga atagggcgtt gagcgaaacc gctgcaccgg
     1021 cggaaagctg gtcccgcgag gacgccagcg agctttaccg catgccggcg tggagcgacg
     1081 gattcttccg catcaacgag cgcggtcaca tcaccgtgca cccgttcgaa gacagcgatc
     1141 tttgtatcga tatcatggac gtcgtcgaag aggccgagcg gagaaacctg acgtttccgt
     1201 tgctgctccg gttccaggac gttttgcgag cgcgtgtgca gcggctgaac gctgcgtttt
     1261 cgtccgccat cgaagaggcc gcctaccaga acgtctaccg cagcgtttac ccgatcaagg
     1321 tgaaccagct gcacgaagtc gtcgaagaag tcctggatgc aggcaagccg ttcgagctgg
     1381 ggctggagtg cggctccaag gccgagctgg tcgccgccct gccgcacctg tcccaggacc
     1441 agacgctcct gatctgcaac ggcgtcaagg accgcaccat gttgtccctg atcctcgcgg
     1501 cgcagggtct tggcaaaaac gtcatcccgg tgatggagaa attcgccgag ttcgaacagc
     1561 tgatggccct ggtggacgag caggagacgg agacgcgctt cggcgtgcgc atccgcctgc
     1621 gtaccgccgg ctccgggcgc tgggcggact ccggtggtta tcgctcgaag ttcggtgtgt
     1681 cgctgccgga actggtggaa ctcgtcgacc agctggagcg cacgaaccgc ctcgactcgt
     1741 ttgttctcct gcactttcac ctgggcagcc agatcgccga catacagacg ctgaagcagg
     1801 cggtgaagga gatggcgcag atttatgccg agctgaaggc gcgcggcctg cccatccgct
     1861 actttgacgt gggcgggggg ttgggtgtca attactccgg cggttatgac gagggcggca
     1921 tcaactacag cctgcaggag tatgcgaatg cggtggttta ctcggtcatg gaagtatgca
     1981 acgcgaaatc ccagccgcac ccggtgctca tctcggagag cggccgtgca atcaccgcgc
     2041 atcactccgt gctggtcgtc gagacgctcg gggcgttccg caaagaccgc gtgccaggag
     2101 atttcaaggt accagccgat gcggcgccga tggtgcgcga cctcggcgat atcctgcaaa
     2161 gactggaggc aaggccggcc agaaaaatga gcgtcagcca gctgctggag gcctatcacg
     2221 acatcgtcga gattcatcgc gaagctgcga ccttgttcag cgtcggttac ctgccgcttc
     2281 agcagaacgc gctggtcgag cgactgtact ggagctgttg cgccgcgatc cttaagcgcc
     2341 tgcgg
//
LOCUS       DASUOM010000593         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_327019,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000593 DASUOM010000000
VERSION     DASUOM010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327019"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFY27_12340"
     CDS             complement(<1..660)
                     /locus_tag="VFY27_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766351.1"
                     /translation="MNNISQILSVIDPTTDDQPALRRAAWVAANTGAELEMLICYYNE
                     YLSGDRLFDSRSLQETRREVLEGYEKFLESLAQPLRESGITITTRAIWDHPLYEGIVR
                     HAAALNADLVFKDAHHHSALSRALLTNTDWSLIRACASPLWLVKSRELPETPVILAAI
                     DPMNEHDKPAALDDEILMLGKSLAQASQAEMHVFHSYDPRVAISTATANAYIPVSLPF
                     DE"
     gene            complement(745..1842)
                     /locus_tag="VFY27_12345"
     CDS             complement(745..1842)
                     /locus_tag="VFY27_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766352.1"
                     /translation="MNSLIRLFAVMRKELRQLGRDRITFAMIVGIPIGQILLFGYAIN
                     TDVRNLSAAVADQANTHLSREFVADLKNTQVLRIGAPAESPEELEALLRTGEISVGVY
                     IPHDFDRRVLDSRPDAHLLVDGADPTILGVANQLVSMPVAFDTWTVAQPLRRSLEVRP
                     YYNPERRTAVNIVPGLIGVILTLTMMLFTAVAIVRERERGNLELLINTPVSNTQLMVG
                     KVVPYIGIGLVQLALIIGVGKLLFDVPIRGSVLDLYLAALAFIAANLALGLFVSTVAK
                     TQFQAMQVTFFILLPSILLSGFIFPFAGMPLFAQYLGELLPITHFIRLTRGIMLREAS
                     LMELSNELWLLVGFTAVAMTAAVMRFSRRLD"
     gene            complement(1839..>2490)
                     /locus_tag="VFY27_12350"
     CDS             complement(1839..>2490)
                     /locus_tag="VFY27_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766353.1"
                     /translation="LEFIADIYSYPRSGRKERIAELLASYDLENVARQLAGTMSGGQR
                     QRLALAAAVLHRPELLLLDEPTSAVDPRSRRDFWAKLFELSDAGTTILVSTHYMDEAE
                     RCHRLAILDRGVKVADGPPAELQENTGMTIIEVRAESPSRAQAELSKLHEVRSVTQLG
                     VRLRVLVPSEVEAPLELVGNELSSAGLHADCSIVGASLEDVFVAVTRDPDSVGRAA"
BASE COUNT          495 a          723 c          805 g          467 t
ORIGIN      
        1 ttcgtcgaac ggaagcgaca cgggtatgta agcattggcc gttgcagttg aaatagcgac
       61 gcgcggatcg taagagtgga agacgtgcat ttcggcctgc gacgcctgcg cgaggctctt
      121 gcccagcatc agtatctcat cgtccagtgc ggcgggcttg tcgtgttcgt tcatcgggtc
      181 gatcgccgcg agaatgacag gcgtctccgg taattcgcgg gatttgacca gccatagcgg
      241 agaagcgcac gcgcgaatca ggctccagtc cgtattcgtc agcaacgcgc gggatagtgc
      301 agagtgatgg tgcgcatcct tgaataccag gtcggcgttc agggcggcgg catgccggac
      361 aatgccctcg tacagcggat ggtcccatat ggcacgggtc gttatggtga tcccggattc
      421 ccgcaacggt tgcgcaagtg attccaggaa cttctcgtac ccttcgagga cttcgcggcg
      481 ggtttcctgc agcgagcgcg aatcgaaaag ccggtcgccg ctcaggtact cgttgtaata
      541 gcagatgagc atctcgagtt cggcgccggt attcgcggct acccacgcgg cgcggcgcag
      601 cgcgggctga tcgtcggtgg tgggatcgat gacgctcagg atctgcgaga tgttgttcat
      661 caggggctgc tctggaggct gacaggccag gatttcatac tagtagaagc gcaagcagaa
      721 gcacaaggct tcggcaagcc gcgcttagtc gagccggcgc gagaagcgca ttacggccgc
      781 cgtcatcgcg accgccgtga acccgaccag cagccacagc tcgttgctca actccatgag
      841 tgacgcttcg cgcaacatga tgccccgggt caggcgaatg aagtgcgtta tcggcagcaa
      901 ctcgcccagg tactgggcaa agagcggcat cccggcgaac ggaaagatga aacctgacag
      961 caatatcgag ggcagcagga taaagaacgt cacctgcatt gcctggaact gcgtctttgc
     1021 cacggtggag acgaaaagcc ccagcgcaag gttggccgcg atgaacgcca gggcggcgag
     1081 gtacaggtcg agaacactgc ctcgtatcgg cacgtcgaac agcagttttc ctacgccaat
     1141 gatgagagcg agctggacca ggccgatccc gatgtagggc actactttgc cgaccatcag
     1201 ttgagtgtta ctgacgggtg tgttgatcag cagctccagg ttgccgcgct cgcgctcgcg
     1261 cacgatagca accgccgtga aaagcatcat tgtgagggtc aggatgacgc cgatcagccc
     1321 cggcacgata ttgaccgccg tgcgtcgctc cggattgtaa taaggacgga cttccaggct
     1381 tcgtcgcagg ggttgggcaa cggtccatgt gtcgaaagca acgggcatcg aaacgagctg
     1441 gttggcgacg ccgaggatgg tcgggtcggc tccgtcgacg aggaggtgag cgtccggccg
     1501 ggaatcgagc acgcgacgat cgaagtcgtg cgggatgtaa acaccaacgg atatttcccc
     1561 tgtccgcagc agagcttcca gttcttcagg gctttccgcc ggtgcgccga tcctcaagac
     1621 ctgggtattc ttaaggtctg caacgaactc ccgcgacagg tgcgtgtttg cctggtcggc
     1681 aactgccgcg gacagattcc tcacgtctgt gttgatcgcg tagccgaaca ggagaatttg
     1741 cccgatggga atgccgacga tcatcgcgaa cgtgatgcgg tcgcgcccga gctgccgcag
     1801 ttccttgcgc atgacggcga acagccgtat cagcgagttc atgcggcacg ccccacggag
     1861 tccggatcgc gggtgacggc tacgaatacg tcttcgagcg acgctccgac gatactgcag
     1921 tcagcgtgca ggcctgcaga tgaaagctca ttgcccacca gctcgagcgg cgcctccact
     1981 tccgagggca ccagcacacg cagcctcacc ccgagctgcg taacgctcct gacctcgtgc
     2041 agcttcgaca gctctgcctg cgcccgcgac ggtgactcgg cgcgaacttc gatgattgtc
     2101 atcccggtgt tttcctgtag ctcagcgggc gggccgtcgg cgactttcac gccccgatcg
     2161 aggatggcga gccggtggca gcgctccgct tcatccatgt aatgggtgga gacgaggatc
     2221 gtcgttcccg catccgatag ctcgaagagc tttgcccaga agtcgcgacg gctgcgcggg
     2281 tctacggcac tggtgggctc atcgagcaac aggagttcgg ggcgatgcag caccgcagcg
     2341 gcaagcgcca ggcgttgccg ctggcccccg ctcatggtgc ctgcaagctg cctggccacg
     2401 ttttcgagat cgtacgaggc cagcagttcg gcgatgcgct ctttgcggcc ggagcggggg
     2461 taggaataga tgtccgcgat gaattcgagg
//
LOCUS       DASUOM010000594         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_807599,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000594 DASUOM010000000
VERSION     DASUOM010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807599"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFY27_12355"
     CDS             complement(<1..680)
                     /locus_tag="VFY27_12355"
                     /inference="COORDINATES: protein motif:HMM:NF044430.1"
                     /note="catalyzes the hydrolysis of dipeptides with a
                     proline at the C-terminal; also catalyzes the low
                     efficiency hydrolysis of organophosphate di- and
                     tri-esters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HEX5766354.1"
                     /translation="MKTREYADTSELATLYPAHIETIQSRHDRALEKAGAGHAVVFSG
                     APKLVFLDDYHYPFKGNPHFIGWLPLTNTPFCYLVHTPGEKPVLVYYQEKDYWHTPPA
                     NPQGFWVGQFDIRIVHELRDVAKHLPKNRDNCILIGDVDEDEHAFGIERINPTTAMNM
                     LHYARGTKTAYELECMRSASRRGVAGHRAAEECFRDGMSEYEIHLAYCRAVSHTESEL
                     PYGNIIAL"
     gene            854..1864
                     /gene="trpS"
                     /locus_tag="VFY27_12360"
     CDS             854..1864
                     /gene="trpS"
                     /locus_tag="VFY27_12360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5766355.1"
                     /translation="MKQDIYLTGITTTGTPHIGNYVGAIRPGIAASKESAKRNYYFLA
                     DFHALAKAEDPEKIHRSTLEIAAAWLALGLDTDNAVFYRQSDIPEIPELTWILTSVTA
                     KGLMNRAHSYKAMVQANEEGGNSDPDRGITMALYSYPILMAADILLFKSTKVPVGRDQ
                     KQHVEMARDIAQRFNHRYGDLFVLPEPEINDNTAVLAGLDGRKMSKSYNNTIPLFADE
                     KQLRKLIMKIKTNSLEPGEPKDTKDSTLFDIYKAFATDAEVRDIEKRFTAGIAWGEMK
                     TILFEYINEQIKPAREEFRRLLDSPSDVERELSKGAERAREVSVPFIAGIRHAIGIRK
                     LG"
     gene            1907..2374
                     /locus_tag="VFY27_12365"
     CDS             1907..2374
                     /locus_tag="VFY27_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5766356.1"
                     /translation="MEFLEAAVEEARQGLSSGGIPIGAVLVLDDEIIGRGHNRRVQRG
                     SAVLHAEMDCLENAGRLTAAEYRRTVLYTTLSPCDMCSGAVLLYGIPRVVIGENSNFR
                     GPEDYLASRGVELEVLDNAVCVRLLKDFIRKHPALWHEDIGEPGAGPGVSAGQ"
     gene            2334..3284
                     /locus_tag="VFY27_12370"
     CDS             2334..3284
                     /locus_tag="VFY27_12370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5766357.1"
                     /translation="MNLALDQAFRPGSKSIADEKAMNKRVKFDFELEFTNGGGMQGQG
                     FRLDIDGDDIPDDQLARYIVRDMRLLMVGQVRILSKEIVTEPHKRGRRESLHDRPAFV
                     DLSHTIEDGLVTYKGLPAPVICDYLSREQSRQVYAAGTEFQIGRIDMVANTGTYIDCP
                     FHRYEHGKDLSEVEIHAFTNLDAIVVRADYRKNLAVDASCFAVREIRNRAVLVHTGWD
                     AHWNTEAYFGDHPYLTEDAAQYLKSCGASLVGIDSLNIDDTRGNARPVHSCLLGAGIL
                     IVEHLCNLGLLPDEGFRFSAIPPKFKGVGTFPVRAMASFD"
     gene            3384..4661
                     /locus_tag="VFY27_12375"
     CDS             3384..4661
                     /locus_tag="VFY27_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX5766358.1"
                     /translation="MGERLRFLLNRLAERLWVKPLAMCILSVVGVLLARMADQAGFSE
                     TVPDISVDSIASLLNVMAASMLMIATFAVGSMVSSYASASSKATPRSFMVVVADDVSQ
                     NALSTFIGAFIFSIVGLVAVSNGYYGEAGRFTLFALTLTVLASVVVTFVRWVDRIARL
                     GRLGSIVDKVEKATADALRQRRRTPTLRALKVEPREHEGEPVCGSTVGYVQHVDVRKL
                     QKQAEKLKSRVRVAALPGTFVAPGRILAYVRSTSGSLPDAALEQVRSAFVIGDGRRFD
                     TDPRFGLVVLSEIAVRALSPAVNDPGTAIDVIGTFVRLFSLWVQSAAEDKIEMGTCDR
                     VEVPEILLADMFDDAFPAIAREGAGAIEVAIRLQKALASLAAAGSEEMTQLATQHARL
                     AFARSEQKLDFPDDLEVLREVARPIVFDERKGP"
     gene            4664..4975
                     /locus_tag="VFY27_12380"
     CDS             4664..4975
                     /locus_tag="VFY27_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX5766359.1"
                     /translation="MHVIIWRFIPRPGMERKFEQQYATGGAWDLLFRKADGFVGTELL
                     RPAGDSTSYLTIDRWRSAAAFADFLRDNEEAYAALDAQFETLTVEEARVGTFSAVQPV
                     Q"
BASE COUNT         1066 a         1412 c         1595 g          965 t
ORIGIN      
        1 ttcagggcga tgatgttgcc gtagggcagt tcgctctccg tgtgtgaaac ggcgcggcaa
       61 taggccaggt gaatctcgta ctcggacatg ccgtcacgga aacactcctc ggccgcgcga
      121 tggccggcga ccccgcggcg cgacgcactg cgcatgcatt cgagttcgta cgccgtcttg
      181 gtgcccctgg catagtgcag catgttcatc gcggtcgtcg gattgatgcg ctcgatgccg
      241 aaggcgtgct catcctcgtc cacgtcgccg atcaggatgc agttgtcgcg gtttttgggc
      301 aggtgcttcg caacgtcgcg gagttcgtgc acgatgcgga tgtcgaactg gcctacccag
      361 aagccctgtg gattggcggg aggggtatgc cagtaatcct tctcctggta atacacgagg
      421 accggtttct cgcccggtgt gtgcacgagg taacagaacg gcgtattcgt caatggcagc
      481 cagccaatga agtgcggatt gcccttgaag gggtaatggt agtcgtcgag aaaaaccagt
      541 ttcggcgcgc ccgagaaaac caccgcgtgt ccggcgcccg ccttctcaag tgcccggtcg
      601 tgccgtgact gtatggtctc gatgtgcgcc gggtatagag tggcaagttc ggacgtatcg
      661 gcgtattcgc gggttttcat gtcacgcata ataacctcaa gcgccgagcg gctgcgagtc
      721 tcctgtcgga gacaaacggc gccacaacgc cgcgacgtgc cgccagcgcc ggtttcaatc
      781 cgcctttaac ctaggcatca tttatcgttt gcacataggg caaacgcagg aacgcgcaaa
      841 agccgacgga tcgatgaaac aggatattta tctcacaggc ataactacaa ccggtacgcc
      901 gcacatcggc aactatgtcg gtgcgatacg acctggaata gcggcaagca aggaaagcgc
      961 gaagcgcaac tactatttcc tcgccgactt tcacgcactc gcgaaggcgg aggacccgga
     1021 aaagattcac cgttcgacgc tcgagatagc ggcagcatgg ctggcgctcg gtctcgatac
     1081 cgacaacgcg gtgttctacc gccagtccga cattccagag atcccggaac tcacctggat
     1141 actgaccagc gttaccgcca agggccttat gaaccgcgcg cactcgtaca aggcgatggt
     1201 gcaggcgaac gaggagggcg gcaacagcga tcccgaccgg ggcatcacga tggctcttta
     1261 cagctatccc atcctgatgg ctgcggatat cctgctgttc aaatcgacga aggtaccggt
     1321 cggcagggac cagaaacaac acgtggaaat ggcgcgcgac atcgcgcagc gtttcaacca
     1381 tcgctacggc gacctctttg tcctccccga acccgagatc aacgacaata cagcggtgct
     1441 ggcaggtctc gacggccgca agatgagcaa aagctacaac aacaccattc cgttgttcgc
     1501 ggatgaaaag cagctgcgca agctcatcat gaagatcaag acgaacagcc ttgaacccgg
     1561 agagccgaag gacacgaagg attcgacctt gttcgacatc tataaggcat ttgcgacgga
     1621 tgccgaagtc agagatatcg aaaagcgctt taccgcgggg attgcctggg gcgagatgaa
     1681 gaccatcctg ttcgagtaca tcaacgagca gatcaagccg gcacgagagg aattccgtcg
     1741 ccttctcgac agcccttcgg acgtagagcg cgagctttcg aagggagcgg aaagggcgag
     1801 ggaggtgtcg gttcccttca tcgccgggat tcggcacgcc ataggaatca ggaagctcgg
     1861 ttgataaccg gttaggtctg ctaaacggta aggaaaaggc aaaagcatgg agtttctgga
     1921 ggcggcggtc gaggaagcga ggcagggcct cagcagcggc ggcattccaa ttggtgcggt
     1981 actcgtgctc gatgatgaga tcatcggccg cgggcacaac aggcgcgtgc aaaggggcag
     2041 cgcagtattg catgcggaga tggattgcct ggagaacgca gggcgcctga ccgccgcgga
     2101 ataccggcga acggtgctct atacaaccct ctctccctgc gacatgtgca gcggggcggt
     2161 cctcctctac gggattccgc gcgtcgtaat cggcgagaac agcaacttcc gcggcccgga
     2221 ggattacctc gcgtcgcgtg gcgtcgaact ggaggtgctg gataatgcgg tatgcgtgag
     2281 attgctgaag gacttcatcc ggaaacatcc cgccctgtgg catgaggata ttggtgaacc
     2341 tggcgctgga ccaggcgttt cggccgggca gtaaatcgat cgcggacgaa aaagccatga
     2401 acaagcgggt gaagttcgac ttcgaactgg agttcacgaa cggaggcggc atgcagggcc
     2461 agggctttcg cctggatatc gacggcgacg atattccgga cgatcagctc gcccgctaca
     2521 tcgtgcggga catgcgcttg ctcatggtcg ggcaagtccg gatcctgagc aaggaaatcg
     2581 tcacggagcc gcacaagcgc ggtcggagag aaagcctgca tgaccgccct gcctttgtcg
     2641 acctgagcca taccatcgaa gacgggctgg taacctataa aggcctcccg gctcccgtca
     2701 tctgcgatta cctgagccgg gagcagtcgc ggcaggtgta cgcagccggt accgaatttc
     2761 aaatcggcag gatagacatg gtcgccaaca cggggacgta catcgactgc ccgttccacc
     2821 gttacgagca cggaaaggac ttgtcggagg tggagatcca cgcgtttaca aacctggacg
     2881 ctatcgttgt ccgcgccgac tatcggaaga accttgcggt cgacgcgagc tgcttcgcag
     2941 tgagggaaat ccgcaacagg gcggtactgg tccataccgg ctgggatgcg cactggaaca
     3001 cggaggccta cttcggggac catccctacc tgacggagga cgcagcgcag tacctgaaga
     3061 gctgcggagc atcgctggtc ggcatcgact cgctcaacat cgacgacacc agggggaacg
     3121 cgagaccggt gcactcgtgc ctgctcggcg ccggtatcct gatcgtcgag cacctctgca
     3181 acctgggcct gttaccggat gaaggcttcc ggttcagcgc cataccgccg aagttcaagg
     3241 gagtcggaac gtttccggtt cgcgcaatgg cgtcgttcga ctgaagcccg tgggagtcgt
     3301 cagcggcaaa ggtttcgggt cgcaggagca acagttgcgc ggcggccgtc aggcgccggg
     3361 gtccggtaaa ggcgggccga accatgggtg agcgcctgcg cttcctgctg aaccggcttg
     3421 ccgagcgcct ctgggtaaag ccgctggcga tgtgcatctt gtcggtggtc ggggtgctgc
     3481 tggcgagaat ggcggaccag gccgggttct ccgaaacggt gccggacatc agcgtcgatt
     3541 ccatagcatc gttgctcaac gtgatggctg cgagcatgtt gatgatcgcg accttcgcgg
     3601 tagggtcaat ggtttcctct tatgcaagcg ccagtagcaa ggcaacgccc aggagcttta
     3661 tggtggtcgt tgcagacgac gtctcgcaga acgccctgtc gaccttcatc ggagcattca
     3721 tcttcagcat tgtggggctg gtggcagtgt cgaacggcta ttatggagaa gcaggacggt
     3781 tcaccctgtt cgctctgacg ttgaccgttc tcgcaagcgt tgtcgttacc tttgtacgtt
     3841 gggtagaccg tatcgcccgg ctcgggcgcc ttggttcgat cgtagacaag gtggagaagg
     3901 ctacagcaga tgcactcagg cagcgacggc gcacgccaac cctgcgtgcc ctcaaggtcg
     3961 agccgcgcga gcatgagggc gagccagtct gcggcagcac ggtaggatac gtgcagcacg
     4021 tcgacgtcag gaagctgcag aaacaggcag agaaactcaa gtctcgggtc agggtcgccg
     4081 ccctccccgg gacttttgta gcaccgggac gcattctggc ctacgtgcgt tccacctcgg
     4141 gaagtttgcc tgatgctgct ttggaacagg ttcgtagcgc cttcgtgatc ggcgacggtc
     4201 gcaggttcga caccgatccg cgtttcgggc ttgtggtgct gtcggagatc gctgtccggg
     4261 cattatctcc ggcagttaac gaccccggta ctgccatcga tgtcatcggg acttttgtcc
     4321 gtctcttctc gctctgggtc cagtcggcgg cagaagacaa gatagaaatg gggacgtgcg
     4381 accgtgtcga ggttcccgag attctgcttg cggatatgtt tgacgatgcc ttcccggcca
     4441 ttgcccgcga aggtgccgga gctatcgaag tcgcaatccg gctgcaaaag gccctggcat
     4501 cactggcagc ggcgggaagc gaagaaatga ctcagctcgc gacgcaacac gctcggctcg
     4561 catttgcccg gtcagagcaa aagctggact tcccggacga cctggaggtc ctgcgggagg
     4621 ttgccaggcc gatagttttt gacgaacgga agggtcccta gccatgcatg tcatcatatg
     4681 gcggttcata ccgcgtcccg gaatggagag aaaatttgaa cagcagtacg caaccggggg
     4741 cgcgtgggac ctgctgtttc ggaaggctga cgggttcgtc ggcacggaac tgttacgtcc
     4801 ggccggcgac agcacgagct acctgaccat cgatcggtgg cgcagcgccg ctgcgttcgc
     4861 agattttctg cgggacaacg aggaagctta cgcggcgctg gatgcacaat tcgagacgtt
     4921 gaccgttgaa gaggcacgcg taggtacttt ttccgctgta caaccggttc aatgatggag
     4981 agacgcatca ccggcttcca ccaggacgaa gaacgagact gggtggctga acttgcct
//
LOCUS       DASUOM010000595         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1111458,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000595 DASUOM010000000
VERSION     DASUOM010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111458"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..673
                     /locus_tag="VFY27_12385"
     CDS             428..673
                     /locus_tag="VFY27_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766360.1"
                     /translation="MRTTVRLDQALLDRARREAAARGMTLTALIEHGLRLALRRPMKT
                     PDRPPVSLPVSRAGGGTCAGVDLDNSAELLDRMDGRP"
     gene            670..1095
                     /locus_tag="VFY27_12390"
     CDS             670..1095
                     /locus_tag="VFY27_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5766361.1"
                     /translation="MILPDVNVLIYAFRSDSEDHTRYKAWLEDVLNGPSPYGIAPQVL
                     ASVVRICTHARIFRDPSTTLEAFEFCRVLLEQPNATVVTPGEHHWFLFHGLCRDAKAS
                     GNLIQDAWFAALAIESACEWITTDRDYARFPGLAWRSPL"
     gene            complement(1108..1791)
                     /locus_tag="VFY27_12395"
     CDS             complement(1108..1791)
                     /locus_tag="VFY27_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5766362.1"
                     /translation="MHFLFLGLVLLGLVFGPGLWVKRVLAKYSEPADRYETTGGELAR
                     YLLDYHGLSAIRVEISDNGDHYDPTEKAVRLTTDKFHGRSLTAIAVAAHEVGHAIQDK
                     AGYRPLRLRHRLVRSFRNVERLGAGILMISPFAGALTRAPSLGILMFLGGLMSLGTAT
                     AVHLVTLPTEFDASFGRALPMLDRLSILKPNDRPHARRLLRAAALTYVAASLMSLLNI
                     ARWWAILRR"
     gene            1862..2044
                     /locus_tag="VFY27_12400"
     CDS             1862..2044
                     /locus_tag="VFY27_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766363.1"
                     /translation="MLLVGDTDEAELHLQQSVEIGEQLGWQSSIACTSGFTSAADAEP
                     RVIFFYVPHRTPRTMT"
     gene            2041..2712
                     /locus_tag="VFY27_12405"
     CDS             2041..2712
                     /locus_tag="VFY27_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766364.1"
                     /translation="MTLSRRLSRTVVVALMTLPAVLFAQESVEKPEMGFFVTSEGLGN
                     GGDLGGLAGADAHCQALADDAGAGHRTWRAYLSTQATGDQAAVNARDRIGSGPWANAN
                     GEVIGADIDDLHYNNANIRYEFALDENGDTVNSGAMSDSPNRHDILTGTRLDGTAFPR
                     GDDRTCGNWTSSDEGAAMVGHHDRFRFTTPGSPWNAAHPSRGCSQEALLDTGGDGLFY
                     CFAAD"
     gene            2798..4036
                     /locus_tag="VFY27_12410"
     CDS             2798..4036
                     /locus_tag="VFY27_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5766365.1"
                     /translation="MKKDCFRLAILLLSLAAPFLPAHGTDYVLTNVNVFNGFEDRILT
                     NTSVFVRNGKIERIGEGTASGGTPLSDGHVVIDCEGYFLLPGLFDVHTHLDSLDRARR
                     ALESGVTTVRTAGVDGFSDVGLRELVRAGKLPGPDVSAAGIFVTPDIGDDILDDPRLA
                     DLAGGVTSDEALRLLVRINADRGVDVIKTRGTERAGRADTDPREQVYTERQLRVIVEE
                     AAKHDIPVLAHAHGDEGARAAVLAGARSIEHGTYLQEETLRLMQKRGTWLVPTYITMD
                     EMNEPKYDYVLRLRAKHMVPQLEKTFKAAHRIGVKIATGADNYYESESINRISLEVAH
                     FVRMGMSNFEALQSATTVSAGLLRLEETTGRIAEGFEADMILVPANPLTDIAALQDVL
                     LVMSNGQIALQRIPFAKQER"
     gene            complement(4137..>4454)
                     /locus_tag="VFY27_12415"
     CDS             complement(4137..>4454)
                     /locus_tag="VFY27_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766366.1"
                     /translation="VLLIAVSLSIYAGVFQLIDGVLIVLANALRGLRDTRSPMWIMLA
                     GYWLVGIGTGALLCFPLGYGAAGLWWGLILGVVAAIALMATRFRRRMAEAELRLKCSK
                     PSL"
BASE COUNT          870 a         1412 c         1385 g          787 t
ORIGIN      
        1 ggtacttctg gttggctggc agccaagcgc atgccgtgtt tgaccagtat ccgatgcacc
       61 gaggagcggg cgatcgtcac ctggtgaaag cgcttgaggt agtcagcgat ccgaccgggc
      121 ccgaagtggt actgctgccg caggtaaagg atcttgctca ccaccgcggg tggggtcgcc
      181 cggggcgacc gcaggggtgt ccgcgggcgg tcggccaggc ccgcctcgcc gtgggcctca
      241 aaccgtcgct ccagcggtag aacagggttc tcgagacgcc gaaatgccgg cacgtccggg
      301 cgacctgtcg cggttctccc tgtgcatatt tcaggatctt catgcgccac gcttgtggat
      361 gaaggaaatg ccgcgcggta gaagcggact ttgacataca gacatatcgt aatgtatgtt
      421 gcgacatatg agaaccactg ttcgccttga ccaggctctg ctcgaccgcg cccgccgcga
      481 ggccgccgca cgcggaatga cgctgacggc tctcatagag cacggtctga ggctggcctt
      541 gcggcgcccg atgaaaacgc cggatcgccc gccggtatcg ttgcctgtca gtcgtgccgg
      601 aggcggtacc tgtgccggtg tcgatctcga caatagcgcg gagctgctcg accggatgga
      661 tggccgtcct tgatccttcc ggacgtcaac gtcctgatct acgcgtttcg ctccgactct
      721 gaagatcata ctcggtacaa ggcgtggctc gaggatgttc tgaacggacc ttcgccatac
      781 ggcatcgctc cgcaggttct ggcaagtgtc gtccgcatct gcacgcacgc gcgaattttt
      841 cgtgatccga gcacgacatt ggaagccttt gagttctgcc gggtccttct cgagcagcca
      901 aatgcgaccg tcgtcacgcc gggcgagcat cattggttcc tctttcacgg gctgtgccgc
      961 gacgcaaagg cgtccggcaa tctgattcag gacgcatggt ttgctgcttt ggccatcgaa
     1021 tcagcttgcg aatggatcac gacggaccgc gactatgcgc gtttcccggg gcttgcctgg
     1081 cgctcgcctc tgtagctgcg caatcgatca gcggcgaagg atcgcccacc accgcgcaat
     1141 gtttagcagg ctcatgagcg acgccgcgac gtaagtcaac gcggctgcac gcagcagtcg
     1201 ccgggcgtgc gggcgatcgt tcggcttgag gatactgagt ctgtccagca tgggcagtgc
     1261 gcgcccgaag ctcgcgtcga attcggtcgg cagcgtcacg aggtgtacgg cagtcgccgt
     1321 gccgaggctc atcagcccgc cgagaaacat gagaatgccg agagacggcg cgcgggtaag
     1381 cgcgcctgca aacggcgaga tcatcaggat gccggcgccg aggcgctcga cattgcgaaa
     1441 cgacctcacc agccgatgcc gcaatcgcag cggtcggtag cctgccttgt cctgaattgc
     1501 gtgcccgacc tcgtgcgcgg caactgcaat cgcggtgagt gagcgtccgt ggaacttgtc
     1561 cgtcgtcagg cgtaccgctt tctccgtcgg atcgtagtga tcgccgttgt cggagatttc
     1621 cacgcgaatg gctgaaagcc cgtggtagtc gagcaggtag cgtgccaatt cgccgccggt
     1681 ggtttcgtag cgatcggcgg gctccgaata cttcgcgagc acgcgtttca cccacagccc
     1741 cggtccgaag accagaccga gcagcaccag gcccagaaac aggaagtgca tgccgcaata
     1801 ataaaggaat cctgcagcgc tgcccgcaat cgcgggggcc gccgacagcc tcggcgtaat
     1861 cgtgctcctg gtcggcgata cggacgaggc ggagctgcac ctccagcaat ccgtcgagat
     1921 cggcgagcag cttggatggc aatcgtcgat cgcctgtacg agcgggttta cctcggcagc
     1981 cgatgccgaa cctcgtgtga tattcttcta tgtgccccac cggacaccca ggacgatgac
     2041 gtgacactat ccagacggct ctcccgtacc gtggtcgttg cattaatgac gttgccggct
     2101 gttctatttg cacaagagag tgtcgaaaag cctgaaatgg gctttttcgt cacgagtgaa
     2161 gggcttggca acggcggcga tctcggcggc ctggccggcg ccgacgcgca ttgccaggca
     2221 ctcgccgacg acgcgggcgc cggccaccga acgtggcgcg cctacctgag cacgcaagca
     2281 accggcgacc aggcagcggt caacgcgcgc gacaggatcg gcagcggacc gtgggcgaac
     2341 gccaacggtg aggttatcgg cgccgatatc gacgatctgc attacaacaa tgcgaacatc
     2401 cgctacgagt tcgccctcga cgagaacggc gacacggtga attccggtgc gatgagcgat
     2461 tcgccgaacc ggcacgacat cctgactggc acgcggctcg acggcaccgc gtttccgcgg
     2521 ggcgacgaca gaacctgcgg caactggacc agcagcgacg aaggtgccgc gatggtaggg
     2581 caccacgacc ggttccggtt cacgacaccc ggcagcccgt ggaatgcggc gcacccgtca
     2641 cgcggctgca gccaggaagc gctgctcgat accggtggcg acggcctgtt ttactgtttc
     2701 gccgccgact agcgcggata cctcaccgat cgcggatcag tcgctcgatc gatattgctc
     2761 gattacccca ccttccagac atcttgtgcg ccctgccttg aagaaagact gcttccgcct
     2821 cgccatcctt ttgttgtctt tggcagcgcc gttcttgccg gcacacggca ccgactatgt
     2881 gctgaccaat gtcaacgtgt tcaacgggtt cgaagatcgc atcctgacga acacctccgt
     2941 gttcgtcagg aacggcaaaa tcgagcgcat cggggaagga accgcaagcg ggggcacgcc
     3001 gctcagcgat ggccacgtcg taatcgattg cgaaggctac ttcctgctgc cggggctctt
     3061 tgacgtgcac acacacctcg acagcctgga ccgcgcccgc cgcgccctcg aatcgggcgt
     3121 taccaccgtc aggacggcgg gcgtggatgg tttcagcgac gtcggcctga gagagctggt
     3181 gcgcgccggc aaacttcccg ggcccgatgt gtcagccgcg ggcattttcg tcacgcccga
     3241 catcggcgac gacattctgg acgatccgcg gctcgccgac ctcgcgggcg gggtcacgtc
     3301 cgacgaggca ctgcgtcttc ttgtccgcat caacgctgac cgtggcgtcg acgtcatcaa
     3361 gacccgcggc acggaacgcg ccggcagggc cgacaccgac cccagggagc aggtatatac
     3421 cgagcggcag ctgcgcgtca tcgtcgagga agccgcgaag cacgatatcc ctgtactggc
     3481 tcatgcccat ggcgacgaag gggcgagggc ggcggtactc gccggtgcgc gcagcatcga
     3541 gcacggcacc tatctgcagg aagagacgct tcggctgatg cagaaacgcg gcacatggct
     3601 ggtaccgacg tacatcacga tggacgagat gaacgaaccg aagtacgact acgtgctgcg
     3661 tctccgcgcc aaacacatgg tgccgcagct cgagaaaacc ttcaaagcgg cgcaccggat
     3721 aggtgtgaag atcgcgaccg gcgcggacaa ctactacgag agcgagtcca tcaatcgcat
     3781 ttcgctcgaa gtggcgcact tcgtccgcat gggcatgagc aacttcgagg cgctgcaatc
     3841 ggcaacaacc gtatcggcgg ggctcctgcg cctggaggaa accacgggac gcattgccga
     3901 gggcttcgaa gccgacatga tcctggttcc cgcaaacccc ctcacggata tcgccgcctt
     3961 gcaggatgtg ctcctggtga tgagcaacgg gcagattgcg ctgcagcgca ttccgttcgc
     4021 caaacaagag agataaaacc tggtgttttc ctccggaccc ccatattcgt ctcgatgctt
     4081 ctgcaagggc tcgtcacggc taccgaggag ctcgtgcgaa cgcatctcgc aatgtatcag
     4141 agcgatggct tgctgcactt caaacgcagc tcagcctccg ccatgcggcg tcgaaaccgg
     4201 gttgccatca gcgcaatggc ggcgaccacc cccaggatca ggccccacca cagcccggcc
     4261 gcgccatagc cgagcgggaa gcacagcaag gctcccgttc cgatgccgac gagccagtat
     4321 cccgccagca tgatccacat tggcgatcgc gtgtcccgca ggccgcgcaa tgcattcgca
     4381 agcacgatca acaccccgtc aatcagctgg aacacgcctg cgtatatcga caggctgacc
     4441 gcgatcagaa ggac
//
LOCUS       DASUOM010000596         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1212671,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000596 DASUOM010000000
VERSION     DASUOM010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212671"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFY27_12420"
     CDS             <1..258
                     /locus_tag="VFY27_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766367.1"
                     /translation="RKVAEGIEEHRACDRKVIPDMHRADPGSIAYSGEAKVLKDYIFH
                     HLQEEEEEMFPRVKKLISAEALETLGSRMQARKRELLNEPA"
     gene            266..1306
                     /locus_tag="VFY27_12425"
     CDS             266..1306
                     /locus_tag="VFY27_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766368.1"
                     /translation="MLSAPERALGAGLKYVDPDSLPLRRLKKGRGFAYVDRDAAIVTD
                     PNLRERLQRLAVPPGWQDVRLARDPRCHIQAVGRDEAGRLQYRYHERWNETGHDVKRE
                     RLENLGHRLGEVRSEIESQLRRHTVDADFALACAIALLDRVGLRIGYPEYSREDGGRG
                     ATTLTRKDVYVKDGTVRLRFFGKGGKRIQRIVSDPTLARALALLRAAPGELLFRWKDN
                     DGVDRCLAAEDVNAALRQRFSDATSAKDFRTFRASSIVAGALQDITSTDLTARKQSLK
                     HSIAESASFLQNTPTVARSSYVHPIVQAAFTDVTFEPAPLFTGQVRAGLTRGETALLR
                     LLKRDLENKAAA"
     gene            1666..3606
                     /locus_tag="VFY27_12430"
     CDS             1666..3606
                     /locus_tag="VFY27_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX5766369.1"
                     /translation="MSRDLFDSYTKAFESRRETVMTIAEYLEGCRNDPLMYAGPHERL
                     LAAIGEPEMVDTSRDSRLGRIHSNRTIRVYPQFSEFYGMEETIERIVAFFRQGAQGLE
                     ERKQILYLLGPVGGGKSSLAERIKALMEDQYIYVLKAGNDLSPVFESPLALFEPETQA
                     EQLQRDYNIPRRRLSGLMSPWALKRLDEFGGDIAKFRVAKVKPSRLRQIGIAKTEPGD
                     ENNQDVSSLVGKVDIRKLEMHSQNDPDSYSYSGGLNRANQGVLEFVEMFKAPIKMLHP
                     LLTATQEGNYAGSENIGSIPFTGIVMAHSNEAEWRTFKNNRSNEAFIDRIYVIKVPYV
                     LRVAEETRIYEKLVRGSELAESPLAPGTLEMLARFSVLSRLKPHENSNLFAKMRVYDG
                     ESLKEVDPKARTLQEYRDAAGVDEGMEGISTRFAFKALSATFNHDTEEVAADPVHLMY
                     VLEQAIKREQFPDDVEGSYLEFIKADLAPRYAEFIGHEIQKAYLESYHDYGQNLFDRY
                     VDYADAWIEDQDFKDPDTGQLLDRELLNQELSKIEKPAGIANPKDFRNEVVKFTLRAR
                     ANNKGRNPSWTSYEKIREVIERRMFSQVEDLLPVISFGSKKDSESEKKHDEFVQRMVE
                     RGYTERQVRRLVEWYMRVKQAS"
     gene            3617..>3732
                     /locus_tag="VFY27_12435"
     CDS             3617..>3732
                     /locus_tag="VFY27_12435"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5766370.1"
                     /translation="MNIIDRRRDPGGKSLANRQRFIRRARALVRRAVREASA"
BASE COUNT          775 a         1159 c         1153 g          645 t
ORIGIN      
        1 cgcaaggtcg ccgaagggat cgaggagcac cgggcgtgcg acaggaaagt cataccggac
       61 atgcatcgcg cggatccggg aagcatcgcc tattccggcg aggccaaggt tctgaaggat
      121 tacatcttcc accatctcca ggaggaggaa gaggagatgt ttccacgcgt gaagaagctg
      181 atctcggctg aggcgctcga aacgctcggc tcgaggatgc aagcgcgaaa gcgcgaactg
      241 ctcaacgagc cggcgtgagg cgacgatgct gagcgcacct gagcgagccc ttggcgccgg
      301 gctgaaatat gtcgatcccg actcgctccc gcttcgacgt ctgaagaaag gcagggggtt
      361 cgcttacgtc gatcgagacg ccgcgattgt gaccgacccg aacctgcgcg agcgcttgca
      421 gaggctcgct gttcctcccg gctggcagga tgtgaggctg gcacgcgatc cgaggtgtca
      481 catccaggcc gttggccgcg atgaggccgg gcggctgcag taccgctatc acgagaggtg
      541 gaacgagacc gggcacgacg tgaagcgtga gcgcctggaa aatctcggtc ataggctggg
      601 ggaggtccgc tccgagattg aatcgcagtt gcggcgccac accgtcgatg cggacttcgc
      661 cctggcctgc gcgatcgcgc tgctagatcg cgtggggctg cggatcggtt atccggaata
      721 ctcgcgagag gacggcggcc gtggtgcaac gaccctcact cgcaaagatg tgtatgtgaa
      781 ggatggcacg gtccggctcc gcttcttcgg aaagggcggc aagcgcatac agcgcatcgt
      841 cagcgatccc actctggcca gggcgctcgc gctgctacgg gcggcgcccg gcgaactcct
      901 cttccggtgg aaggacaatg atggggtgga ccgctgtctg gctgctgagg acgtcaacgc
      961 tgcactgcgg cagcgtttca gcgacgccac cagcgccaag gacttccgta cctttcgggc
     1021 cagttcgatc gttgctggcg cgttgcagga tatcacctcg accgacctca cggcccgcaa
     1081 gcagtcgctc aagcactcga ttgccgagag cgccagcttt ctgcagaata cgccgacggt
     1141 tgcgcgctcc agctatgtac atccgatcgt tcaggcagcg ttcacggatg taacatttga
     1201 gccggcgccg ctgttcaccg gccaggtgag ggcaggattg acccgtggcg agaccgcgct
     1261 tctgcgactg ctcaaacggg atctggaaaa taaggccgct gcgtgatcgt ccagcgcgcg
     1321 tgtgtccgag gcatcacgtt gggcagcctc cagcggcggc ttacttcaaa taatgatgaa
     1381 agctggagcc aacgaccgga ttcttctatt attctgattc atgcgtcggg gaagttagag
     1441 cagtcgccct cttcaattcg tcgccgtggc gccgacatct gaagagagtt cctcaccgca
     1501 ccaccggggc agcgtcgccg cacccgctac gtggcagacg tgctactccc gtcagggtgc
     1561 agcagacgag gggacagctc gaaacgcgac gacgcggcga gcaactgcac gtgcctcttg
     1621 ccggttatgt gtctgaccgg tcggtctttg gacatgggag caacgatgtc gcgcgatctg
     1681 ttcgacagct acaccaaggc cttcgagtcc cgtcgcgaga cggtgatgac gattgccgag
     1741 tacctcgaag gctgccgcaa tgatccgctg atgtacgcgg gaccacacga gcgcctgctg
     1801 gccgcgatcg gcgagcccga gatggtcgat acatcgcgcg acagccgcct cggccgcatt
     1861 cattcgaacc gcacaattcg cgtctatccg caattctccg aattctacgg gatggaagag
     1921 accatcgagc ggatcgtcgc gttcttccgg cagggcgccc aagggctcga agagcgcaag
     1981 cagatcctct acctgctggg tcctgtcggc ggcggcaagt cttcgctcgc cgagcgcatc
     2041 aaggcgctga tggaagacca gtacatctat gtcctcaagg ctggcaacga cctcagcccc
     2101 gtcttcgaaa gcccgcttgc cctgttcgaa cccgaaacgc aggccgaaca actgcagcgc
     2161 gattacaaca ttccgcgccg ccgtctcagc ggactgatga gtccgtgggc tctgaagcgc
     2221 ctcgacgagt tcggcggcga cattgccaaa ttccgcgtgg cgaaggtgaa gccgtcgcgg
     2281 ttgcgccaga tcggtatcgc caagaccgag cccggcgatg agaacaacca ggatgtctcc
     2341 tcgctggtcg gcaaggtcga tatccgcaag ctggagatgc actcgcagaa cgatccggat
     2401 agctattcgt attccggcgg cctcaaccgc gccaaccagg gcgttctcga attcgtcgag
     2461 atgttcaagg cgccgatcaa gatgctgcac cccctgctca cggcgacgca ggaaggcaac
     2521 tacgccggct cggaaaacat cggctcgatc ccattcaccg gcatagtcat ggcccactcg
     2581 aatgaggccg aatggcgcac gttcaagaat aaccggtcca acgaagcctt catcgaccgc
     2641 atctacgtca tcaaggtgcc gtacgtcctg cgcgtcgccg aagaaactcg tatctacgaa
     2701 aagctggtcc gcggctcgga gctggcggaa tcgccgctcg cgccgggcac tctggaaatg
     2761 ctggcgcgct tctcggtgct ctcccgcctc aagccgcacg agaactccaa tctcttcgcc
     2821 aagatgcgcg tctatgacgg ggaatccctc aaggaggtcg atcccaaggc gcgcacgttg
     2881 caggaatacc gcgatgccgc tggcgtcgac gaaggtatgg aaggcatctc cacccgcttc
     2941 gccttcaagg cgctgtcggc gacgttcaac cacgacaccg aggaagtcgc cgccgacccg
     3001 gtgcacctca tgtatgtgct cgaacaggcg atcaaacgcg aacagttccc tgacgatgta
     3061 gagggatcat acctcgaatt catcaaggcc gacctcgcgc cgcgctatgc cgagttcatc
     3121 ggccacgaga ttcagaaggc gtatctcgag agctatcacg actacggcca gaacctgttc
     3181 gaccgctatg tcgactatgc cgatgcctgg atcgaggacc aggacttcaa ggaccccgac
     3241 accggccagc tgctcgaccg tgaactcctc aaccaggagc tgtcgaagat cgagaagccc
     3301 gccggcatcg ccaacccgaa ggacttccgc aacgaggtcg ttaagttcac gctgcgggcg
     3361 cgcgccaaca acaaggggcg caatccttcc tggaccagtt acgagaagat ccgcgaagtt
     3421 atcgagcggc gcatgttcag ccaggtagag gacctgctgc cggtgatctc gttcggctcg
     3481 aagaaggata gcgaaagcga gaagaagcac gacgagttcg tgcagcgcat ggtcgagcgc
     3541 ggctataccg agcgccaggt gcgccgcctg gtcgaatggt acatgcgggt gaagcaggcc
     3601 agctagaaag cacccgatga acatcatcga tcgccgtcgg gaccccggag gaaagtcgct
     3661 ggcgaaccgc cagcgattca tccgccgcgc ccgcgccctg gtccgccgcg ccgtccgcga
     3721 agcctccgcc aa
//
LOCUS       DASUOM010000597         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_327577,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000597 DASUOM010000000
VERSION     DASUOM010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327577"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..890)
                     /locus_tag="VFY27_12440"
     CDS             complement(48..890)
                     /locus_tag="VFY27_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEX5766371.1"
                     /translation="MSDFLRVMADRSEQRAASIRNAFSASDFDHPVCPLTLRGFDLVA
                     EIKMRSPSEGQLSREHAGNAERALQYAEGGAAAISVLTEPSRFGGHMSHLRDVVAAVK
                     SRRIPVMRKDFLVDGRQVLEARAAGASGVLLIAAMLQDTQLQRMLDCAFEHSLFVLLE
                     SFDADDVERTRRLLELPQYADKAAQRQLLVGVNSRNLRTLEVDPDRLAKLSHSLPEGA
                     VAVAESGLNDAGDAASVSALGYRVALVGTALMRAPDPAALIGEMLAAGRAAGTSLQGN
                     SKTA"
     gene            complement(887..1915)
                     /gene="trpD"
                     /locus_tag="VFY27_12445"
     CDS             complement(887..1915)
                     /gene="trpD"
                     /locus_tag="VFY27_12445"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5766372.1"
                     /translation="MSAANTRLLDRLLDGQSLEEAEAYDLMHALADGDVHPVLAGALL
                     AALRAKGETADEIRGFATAMRQLALHPLVPPGAAIVDTVGTGGDGSGSFNLSTGTALL
                     AAACGARVVKHGNRSVSSRCGSADLLERLGMPLPLDEQAAMACLDSLNFTFLFAPAYH
                     PAMKMVAPVRGAMGVRTVFNLLGPLTNPAAPPYQLIGAFSRHAAKLMAETLSGMPIER
                     AFVVHGEPGWDEASPVGEFLLYDVRPGFVTATVRHPESYGLERCQAGDLAGGDAEHNA
                     RELRRVFCGEDHGPHRGALLMGASLVLEVTGLAGNELDGAAMAAETLDSGKAEAFLES
                     FEAQFAAA"
     gene            complement(1912..2502)
                     /locus_tag="VFY27_12450"
     CDS             complement(1912..2502)
                     /locus_tag="VFY27_12450"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5766373.1"
                     /translation="MTLRAVRILLIDNYDSFTYNLVQAFAAMGAEVLVYRNDAVSVDE
                     ARNLNFSHLVISPGPGRPEDAGVSLRMIDAFASQVPVLGVCLGHQCLVSHFGGDIVRA
                     GRLMHGKTSLVTHDGRTIFHGLSKPFEAGRYHSLCAERESLPAELELSAETNEGEIMA
                     VRHRSLPLEGVQFHPESVLTPEGNLLMANFMRMGNA"
     gene            complement(2499..>3717)
                     /locus_tag="VFY27_12455"
     CDS             complement(2499..>3717)
                     /locus_tag="VFY27_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5766374.1"
                     /translation="RNAFHVNGSRHPFPESKDEYLGHLRSALEMAPQPRPMVPGLPFT
                     GGLVGVSGYDVVRLFERLPNGAAAQPGIPLAAFTATSSTLVFDHLTRRVALLHDGSES
                     DRRRLRREVITLLRGPVPPNGERVSISVPEPCMHEHGFIERVAVCKEHIAAGDIYQIV
                     LSVLFRGRGNVAPFEVYRALRLLNPSPYMFYFEFGDLKVVGSSPEALVKLHGRHASLR
                     PIAGTVPRGRTAQEDTRNEKTLLDDPKESAEHVMLVDLARNDLGRVASPGTVRVEPWR
                     AIERYSHVMHIVSGVQGELAPQFDAFDLFAASFPAGTLVGAPKVRAMEIIDELEDTGR
                     GLYAGTAGYYGIGGDMDQAITIRTLVFNGDEYTFQAGAGIVADSVPVKEYQEVLAKGA
                     ILKSALEMAGEGL"
BASE COUNT          669 a         1257 c         1122 g          669 t
ORIGIN      
        1 ctcgacgctg ctcacgtgcg tcaggccaca tatcttgacg agacgtttca tgccgttttc
       61 gaatttccct gaagtgaagt tcctgccgcc ctgccagccg ccagcatttc accgatcagc
      121 gcggcaggat ccggtgctcg catgagcgca gttcccacga gggcgacacg ataacccagc
      181 gccgataccg atgccgcgtc tccggcatcg ttgagcccgc tttcggcaac cgcgaccgcg
      241 ccttccggca gcgagtgcga cagctttgcc agtctgtcgg ggtcgacttc cagcgtgcgc
      301 aggttccggc tgttgacacc gaccagcagc tgcctttggg cggctttatc cgcgtactgc
      361 ggcaactcca gcaatcttcg cgttcgctca acgtcatcgg catcgaaaga ttccaacagc
      421 acgaacaggg aatgctcgaa agcgcaatcg agcatgcgct ggagctgcgt gtcctgcagc
      481 atggcggcta tcagcaatac accgcttgcg cccgccgcac gtgcttccag tacctgccgt
      541 ccatccacca ggaaatcctt gcgcattacc ggtattcgcc tgcttttcac tgcggcgaca
      601 acgtcgcgca ggtgagacat gtgaccgccg aatcgtgacg gctcggtgag aacggatatc
      661 gcggccgcac caccctccgc atattgcagc gcccgctcgg cattgcctgc atgctcgcgc
      721 gacagctgtc cttcagacgg cgatcgcatc ttaatctcgg cgaccaggtc gaagccccgg
      781 agggtgagcg ggcacaccgg gtggtcaaag tcgcttgcgg aaaacgcatt ccggatcgac
      841 gcggcacgct gctcgctgcg atccgccatg acgcgcagga agtcgctcat gccgcggcga
      901 actgcgcctc gaagctttcg agaaacgctt ccgccttacc gctgtccagt gtctccgccg
      961 ccatcgccgc gccatccaac tcgtttccgg cgagtcccgt aacttcgagc accagcgaag
     1021 cacccatcag cagcgccccg cggtgcggac cgtggtcttc accgcagaag acgcgccgca
     1081 gttcccgtgc gttgtgctct gcgtcgccac ctgcgaggtc tcctgcctgg cagcgctcca
     1141 agccgtaact ttcgggatga cggacggttg cggtgacgaa accgggccgg acatcgtaaa
     1201 gcaggaactc gccgaccggg gaagcctcgt cccagcccgg ctcgccatgc acgacgaagg
     1261 ctcgctcgat cggcattccc gacaacgtct cggccatcag cttcgcagcg tgccggctga
     1321 atgctccgat cagctggtaa ggcggtgccg ccgggttggt gagtggcccg agaagattga
     1381 acacggtgcg aacgcccatg gcacccctca ctggcgcaac cattttcatt gcggggtgat
     1441 aggcaggcgc gaacaagaaa gtgaagttga gggagtcaag gcacgccatc gcagcctgct
     1501 cgtcgagcgg caacggcatg ccgagccgct ccagcaggtc ggcgctgccg cagcggctgg
     1561 aaacggagcg gttgccgtgc ttgacgaccc ttgcgccgca ggccgcggcg agcagcgcgg
     1621 taccggtcga gaggttgaaa ctgccggaac cgtcgccgcc cgtgccgacg gtgtcgacaa
     1681 tcgcagctcc gggcggcacc agggggtgca acgcaagctg cctcatcgcg gtggcgaatc
     1741 cgcgaatctc gtccgcggtc tcccccttgg cgcgcagcgc cgcaagcaag gcgcccgcga
     1801 gcacgggatg tacgtcgccg tccgccagcg cgtgcatgag atcgtatgct tcagcttcct
     1861 cgagcgattg cccgtccagg agccggtcga gcaaccttgt attcgcagca ctcatgcatt
     1921 ccccatccgc atgaagttgg ccatgagcag gttaccttcc ggcgtcagga cgctttcggg
     1981 atgaaactgc accccctcta gcggcagcga ccggtggcgc accgccatga tctccccctc
     2041 gttagtctcc gcgctcagct ccagctctgc cggtagcgac tcccgctccg cgcaaagcga
     2101 atgatacctg ccggcttcga acggtttcga caagccgtgg aaaatcgtgc ggccatcgtg
     2161 agtaacgaga gacgtcttgc cgtgcatcag ccgcccggcg cgcacgatgt cgcccccgaa
     2221 atggctcacg aggcactggt gccccagaca cacgccgagg acgggtacct ggctggcgaa
     2281 ggcatcgatc atcctgagcg atactcccgc atcctccggt cgtccgggac cgggtgagat
     2341 gacgagatgg ctgaagttca agttcctcgc ctcgtcaacg ctgacggcat cgttgcggta
     2401 caccagcact tcggcgccca tcgccgcgaa cgcctgcacc aggttgtagg tgaacgaatc
     2461 gtaattgtcg atcagcagta tgcgcaccgc ccgcagcgtc acaatccttc tcccgccatc
     2521 tcgagcgcgc tcttcagtat cgcccccttc gccagaactt cctggtattc ctttaccgga
     2581 acgctgtccg cgacgatccc cgcgccggcc tggaacgtgt attcgtcacc gttgaacacc
     2641 agtgtcctga tcgtgatcgc ctggtccatg tcgccgccga tgccgtagta gccggcggtg
     2701 ccggcataga gaccgcggcc ggtgtcctcg agttcgtcga tgatctccat ggcgcgcact
     2761 ttaggcgcac cgaccagcgt gcccgccggg aaactcgcgg cgaaaaggtc gaacgcatcg
     2821 aattgcggcg ccagttctcc ctgcacgccg cttacgatgt gcattacgtg gctgtaacgt
     2881 tctatcgcgc gccagggttc cacgcgcacg gttcccggcg acgcgacgcg gccgaggtca
     2941 tttcgcgcga ggtcgaccag catcacgtgc tccgccgatt ccttcgggtc gtcaagcagc
     3001 gtcttctcgt tccgcgtgtc ctcctgcgcc gtcctgccgc gcggcacggt gccggcgatc
     3061 ggccggagcg acgcatgcct cccgtgcagc ttcaccagcg cctcgggcga ggagcctacg
     3121 accttcaggt cgccaaactc gaaatagaac atgtaaggag acggattgag caacctcagt
     3181 gcccggtaca cctcgaaagg agcgacgttg cctcgccccc tgaagagcac cgacaggacg
     3241 atttggtaga tatcccctgc cgcaatatgt tccttgcaga cagcgacacg ctcgatgaat
     3301 ccgtgctcgt gcatgcacgg ttcgggcacg ctaatcgata cgcgttcgcc atttggtggc
     3361 acgggcccgc gcagcagggt gattacctcg cgccgcaggc gccggcgatc gctttcggag
     3421 ccgtcgtgca gcaaggccac gcgccgcgtc aggtggtcga aaacgagggt cgagctggtt
     3481 gcggtgaagg ccgccagggg gatccccggt tgcgcagccg cgccgttggg cagtcgctcg
     3541 aagaggcgca ccacgtcgta acccgagacg cctaccagcc ccccggtgaa tggcagcccc
     3601 ggcaccatgg gcctcggttg cggtgccatc tcgagggccg accgcaaatg accgaggtat
     3661 tcatccttgc tctccggaaa aggatgcctg ctgccattta catggaatgc gttccgg
//
LOCUS       DASUOM010000598         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1332577,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000598 DASUOM010000000
VERSION     DASUOM010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332577"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFY27_12460"
     CDS             <1..1132
                     /locus_tag="VFY27_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5766375.1"
                     /translation="LMPGLAEMHAHIPGSGDRRWAENVLFLYVSNGVTTIRGMAGSPL
                     HLELREQTENGELVGPAIYAAAPGISGNNAPAPEDAERAVRERANAGYDLLKVFEMPV
                     ESYERVAQTAHEIGIPFAGHVPETVGLVGALEAGQASIDHLDRYVEFLAPGYADMPGR
                     EAGFFASGLVDIADTKRIPEAVERTLAAGTWNVPTLSLVEHLASPEPAEQMIRRPEMR
                     YMPPGVLDDWVSAKTEFRARPDFQPEAAERLVEIRRQLTNALHDAGAPLALGSDAPQF
                     FNVPGFSIHHEMAMMVEAGLSPYEVLVTGTRNPAAYFGTPEEFGTVAVGRRADLILLE
                     ANPLDDVGNVKRRTGVMVRGRWLPEAEIRERLERIARLVGAQ"
     gene            complement(1219..1542)
                     /locus_tag="VFY27_12465"
     CDS             complement(1219..1542)
                     /locus_tag="VFY27_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766376.1"
                     /translation="MYARQVSTDLKPNSKAEFTQKMEAEILPLLRKQKGFKDAITFVD
                     KAGKEAFAVSLWDSKENADAYNSGSYAEVTRLLSKLVDGTPRVKAFEVANSTFHNIGA
                     KLKAA"
     gene            1722..>2047
                     /locus_tag="VFY27_12470"
     CDS             1722..>2047
                     /locus_tag="VFY27_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5766377.1"
                     /translation="MDVASVIKARLKELGVGQKELAAAAEVTDSYVSQLLTRKKLPPA
                     PDRTDIYEKMATFLRLSNGELATLARAQRLEILKRGFEERPAPLLKNVRELILRKCIP
                     EKQQQIR"
BASE COUNT          398 a          609 c          637 g          403 t
ORIGIN      
        1 cctgatgccg gggctcgcgg agatgcacgc gcacattccc ggttccggtg atcgacgttg
       61 ggcggagaac gtcctgttcc tgtacgtctc caacggcgtc acgaccatcc gcggcatggc
      121 gggcagcccg ctgcacctcg agctcaggga gcagaccgag aacggggagc tggtgggccc
      181 tgccatctac gccgcggcgc ccggcatcag cggcaacaac gcaccggcgc ccgaggatgc
      241 cgaacgcgcc gtgcgcgagc gggcgaacgc cggctacgac ctgctgaaag tcttcgaaat
      301 gccggtagaa agctatgagc gcgtggcgca gacggcgcat gagatcggca tccctttcgc
      361 cggtcacgtt ccggaaactg tcgggctggt cggggcgctc gaggcaggcc aggcctccat
      421 tgatcatctc gatcgatacg tcgagttcct tgcgcccggc tacgcggaca tgccgggcag
      481 ggaggcgggg ttctttgcca gcggcctggt cgacatcgcc gacacgaagc gcattcccga
      541 agcggtcgag cgtacgctcg cggcgggaac gtggaacgta ccgacgctca gtctcgtcga
      601 gcatcttgcc tcgccggaac ccgccgagca gatgatccgg cggccggaga tgcgttacat
      661 gcctccgggc gtgctggacg actgggtaag cgcaaaaacg gagtttcgcg cgcgaccgga
      721 cttccagcct gaagcggccg aacggctggt ggaaattcgc cggcagctga cgaacgcttt
      781 gcacgacgcc ggcgcgccgc tcgcgctcgg ttcggatgct ccacagttct tcaacgtccc
      841 cggcttttcc atccaccacg agatggccat gatggtcgag gccgggctat cgccttacga
      901 ggtgcttgta accggcacgc gcaaccccgc cgcgtacttc ggcacaccgg aggaattcgg
      961 gacggtcgcg gtcgggcgac gggcggacct catcctgctc gaggcgaatc ctctcgacga
     1021 cgttggcaac gtcaaacgcc gtaccggcgt aatggtacgg ggacggtggc tgccggaagc
     1081 cgaaatccgg gagcgcctgg agcgcattgc ccgcctggtc ggcgcgcagt aaatggctag
     1141 tgagctcggc gtcgtcattc gccacgaaaa aagccgacga agcgagcctc gtcggctgca
     1201 ggggtttacg ctgggacttt acgcagcttt cagcttggcg ccgatgttat ggaaggttga
     1261 attggcgacc tcgaaggctt taacccgcgg ggttccgtca accagtttgg acagaagcct
     1321 ggtcacttcg gcgtaactgc cgctattgta cgcgtccgca ttttccttgc tgtcccacag
     1381 gctcacggcg aacgcttctt tgccggcctt atcgacaaag gtaatcgcgt ccttgaatcc
     1441 cttttgcttg cgcagcaggg gaaggatttc cgcctccatt ttctgggtga attcggcttt
     1501 gctgttcggc ttcaggtccg ttgatacttg acgtgcatac atatcgttct cccgtccttg
     1561 tgattggccg gaaagggcgc ttcagataac caacttggtt gattcgttcg gaagccctct
     1621 cggtccagtt accataatgg gttaattcac ttgtatagtc aagttctatc tgagatcatt
     1681 ctttcaatta accacttaaa gtcaatccag gcaggattcg aatggatgtt gcgtcggtca
     1741 taaaagcgcg actgaaggag ttgggcgtgg gccagaagga actggccgcc gcagccgagg
     1801 tgacggactc ctatgtttcc cagctactaa cccggaagaa actgccgcct gctccggacc
     1861 gaaccgatat ctacgagaag atggcaacgt ttctacgcct ctcgaacggc gagttggcga
     1921 cactcgctcg cgcgcagcga ctggaaatat tgaagcgcgg attcgaagag cggcccgcgc
     1981 ctttgttaaa gaacgtgcga gaactcatac tgcggaagtg cattccagaa aagcagcagc
     2041 agatccg
//
LOCUS       DASUOM010000599         4527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1171800,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000599 DASUOM010000000
VERSION     DASUOM010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171800"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFY27_12475"
     CDS             complement(<1..439)
                     /locus_tag="VFY27_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766378.1"
                     /translation="MEQTVPASAGSGFAKANPHIAILLCLIIAPQGHAESIALIKSYD
                     FAPYDAAMSGFMSACSNDITEYNLDGEADGNARLVQEIKSDNPDLIVAIGVLAAQFAR
                     QELGEFAVLYVMVPKLQTEGLTGDNIGGISLDIPIDQQLDVYRS"
     gene            complement(498..1703)
                     /locus_tag="VFY27_12480"
     CDS             complement(498..1703)
                     /locus_tag="VFY27_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5766379.1"
                     /translation="MLCAIPRIAAAGILLLASSHALADKLNTLIPGLYGGDGITLAGV
                     DDGPFPSHAPHFLVESADTFNRLNDLISAQIGAIPFASSGAGFAFRYDEDLGTFVQVA
                     TSFGPIYAERADTIGKGRFNANLSITSFKYDEFLGDDLDSLIVTTFHEPDTLPPDDEH
                     TSFELDTVDIDLNLDIRVNALVFAGTYGLTDRLDVGFLVPFSEVDVRIGAHAEVVKSP
                     LNQIPVDVHTFEGGPESPDDSASGSARGLGDILLRAKYHWVKSDQHNLAVALQMKSAT
                     GDEDDFLGTGEATVRPLLIYSRTFGSFTPHVNLGYEFNLDDSDQNSLEYAAGFDVGTE
                     NLTFALSVLGSREASGDGIGDTIVSAAAGLKWNPFGSYIFTGNLLVPLNDDGLRSDLI
                     TTLAFERNF"
     gene            1961..3919
                     /locus_tag="VFY27_12485"
     CDS             1961..3919
                     /locus_tag="VFY27_12485"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766380.1"
                     /translation="MFGELKAWIRYQLDGIFARSSISQFVLLVLLSLVIVLLGMTAYF
                     FGLFSPQNQEVEGIGSRIDRGLWDSLWWSLKHLIEPGVFADNYGATAPVVLISLLIAI
                     MGMVIFGILIGFISTSMESRLAALQKGNSPVKERGHILILGWSNKVIMLLRLLTRLKT
                     GLRIVVLARPEIGHMQEALRAEGGLALPIKLVLRTGVPTNILELRRVAFEQASSIIVL
                     ADSARESDAAGRDVEAIKTAMLLSSFTGWRGPKPVLVSEINRKQNVEIARIAGRNEIS
                     VVSSSEVISKVIVQSARQSGLSFVYSEILSGAGNSIRVQRVPACAGKRFGDIQAGFAD
                     AVPIGVSWQQEKDGVPRYVAGLNPEPDYVIDADDSLVLLCRGNKASYDANAKPEIPAA
                     ARSESYRKPPLNRILVLGFNDNVQDILIEFDGHVSSGSHIDLVSNLEDEEARQRLEQN
                     LTKPFRNIEVAFRHGSTVSAAFLRSLDVLSYDCIITLADESNEDDPDARSIMLLLLLS
                     DIITSDPSSSRINVVSEIHDPANRELVARTAAQEVIVSPEIISMQLSQISQQPVLESI
                     YQELLSAGGIEICIKPASRYVDLLKPCSFIDLQLAAQRMMEVVIGVRIHDPAAVARHK
                     VQLNPRRETRWEFGPNDSVVVLAQEIYD"
     gene            3959..4336
                     /locus_tag="VFY27_12490"
     CDS             3959..4336
                     /locus_tag="VFY27_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766381.1"
                     /translation="MGKILLVEDNQMNRDMLSRRLRRKGFEVLLATDGQEGISLARSG
                     APDLILMDMSLPTMDGWTAARLLKSDSDTRSIPIIALTAHAMRGDRDKALDAGCDEYE
                     TKPVNLARLLGKIESLTRSPLSP"
     gene            complement(4342..>4527)
                     /locus_tag="VFY27_12495"
     CDS             complement(4342..>4527)
                     /locus_tag="VFY27_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5766382.1"
                     /translation="TSMAAPHVSGVVAAFLSIRREFIGQPDKVKQILLTNCTDLGRDR
                     PMQGAGLPNLVRMLVGT"
BASE COUNT          903 a         1291 c         1407 g          926 t
ORIGIN      
        1 tcgagcgata aacgtcgagc tgctggtcga tcggtatgtc cagtgaaatg ccgccgatgt
       61 tgtcgccggt cagcccttct gtctggagct tcggaaccat aacgtacagt actgcgaatt
      121 cgcccagttc ctgtcgtgca aattgcgcgg ccagcacgcc gatcgcgacg atcaggtcag
      181 ggttgtcgct cttgatttcc tggaccagcc tcgcgttgcc gtcggcttca ccgtccaggt
      241 tgtattccgt tatgtcattg ctgcaggcac tcatgaagcc ggacattgcc gcgtcgtagg
      301 gagcaaaatc gtagctcttg atcaacgcga tcgactcggc atggccttgc ggcgcgataa
      361 tgaggcaaag cagtatcgcg atgtggggat ttgccttcgc gaacccggag cccgcggatg
      421 cagggacagt ttgttccacg gcaatcacgt accggcttct gtcggcttac cagggcatgc
      481 ccgttccagt ttcatcgtca gaagttgcgt tcgaaggcga gggtagtgat gagatccgaa
      541 cgaagaccgt cgtcgttcag gggcaccagc aggttgcccg taaagatata gctaccgaat
      601 gggttccact tgaggcccgc cgcggcgctg acgatggtat cgccgatccc gtcaccgctg
      661 gcttcgcggc tcccgagcac gctcagggca aaggtcaggt tttcggtccc gacatcgaaa
      721 cctgcggcgt actccaggga gttctggtcc gaatcatcca ggttgaactc gtatcccagg
      781 ttcacgtgtg gcgtgaacga gccgaaggtg cgggagtaaa tcaacagggg ccgtaccgtc
      841 gcctcaccgg ttcccaggaa gtcgtcttca tccccggtag cgcttttcat ttgcagggca
      901 acggcgaggt tgtgctggtc gctcttcacc cagtgatact ttgcccgcag cagtatatcg
      961 ccgaggccgc gagcgctgcc gcttgcgctg tcgtccggcg attccgggcc gccttcaaac
     1021 gtatgtacat cgaccgggat ctgattgagc ggcgacttta cgacttctgc gtgtgcaccg
     1081 attcgcacgt ccacttccga gaacggaacc aggaatccca catcgagccg gtccgtcaat
     1141 ccataggtgc cggcaaaaac gagggcattg acccggatgt cgaggttcag gtcgatgtcc
     1201 accgtatcca gctcgaagga tgtgtgctcg tcgtcaggcg gaagagtatc gggttcatga
     1261 aaggtcgtaa cgatcaggct gtccaggtcg tcgccgagga actcgtcgta cttgaaggat
     1321 gtgatggaca agtttgcgtt gaaccgaccc ttaccgatgg tatcggcgcg ttcggcatag
     1381 atcgggccga agctcgttgc aacctgaacg aaggtgccca ggtcctcgtc gtagcggaag
     1441 gcaaaaccgg cgccggacga tgcgaacggg atcgcgccga tctgcgccga aatcaggtcg
     1501 ttcaggcggt tgaaggtgtc tgcgctttcg accaggaaat gcggtgcatg ggacgggaac
     1561 gggccatcgt ctactccggc aagagtgata ccgtcacccc cgtaaagacc ggggatcagg
     1621 gtattcagtt tgtcggcgag tgcgtgggac gaggccagca gcaggatgcc ggcggcggca
     1681 attcgaggaa tggcgcaaag catgtcgtac ctccccgggg cgtggcgatg gaggcgaatc
     1741 cttaaggatt tcgcggcctc ccacagcctt aggtgtcctt gttattgtca cccaatcaag
     1801 gtttttgtcg agcgaattat ataccagccc atggtgcgcc gccgcagggt acgaaccacg
     1861 tccatgcgga cgacggcaac cgggaatccg taaatcggtg aggtattcgg gctaatattg
     1921 cgctccgctc gtataaagcc gtggcttctt ggggagtgcc atgttcggcg agttgaaagc
     1981 atggatccgg tatcagcttg acggcatctt cgcccggagc tcgatatcgc agttcgttct
     2041 ccttgtcctt ttgtcgctgg tcatcgtcct gctcgggatg acggcctatt tttttgggct
     2101 gttctccccg cagaaccagg aagtcgaggg catcgggtca cgaatcgacc gggggctatg
     2161 ggactcgctt tggtggtccc tgaagcacct gatcgaaccg ggagtgttcg cggacaacta
     2221 cggtgcgacg gccccggtcg tcctcatatc cttgctcatc gcgattatgg gtatggtcat
     2281 attcggtatc ctgatcggct ttatctccac gtccatggag agccgcctcg cagcgctcca
     2341 aaagggaaac agtccggtga aggagcgcgg gcacatcctt atcctgggct ggagcaacaa
     2401 ggtgatcatg ctgctgcgct tgctcacgcg gttaaagacg gggctgcgga ttgtcgtcct
     2461 ggcgcgtcct gaaatcgggc acatgcagga ggcgcttcgc gcggagggag ggttggccct
     2521 gccgatcaaa ctcgtcctgc gcacgggcgt gccgacgaac atactggaac tcaggcgggt
     2581 tgcgttcgaa caggcaagca gcatcatcgt gctggccgat tccgcgcgtg aatccgacgc
     2641 ggccggcagg gacgtcgagg ccatcaagac ggccatgctg ctttcatcgt tcacgggctg
     2701 gcgcggaccg aagccggtcc tcgtatctga aatcaaccgc aagcaaaacg tcgagatcgc
     2761 gcggattgca ggccgcaacg aaatctccgt ggtttcgagc agcgaggtga tcagcaaggt
     2821 tatcgtgcaa agcgcccggc agagcggtct gtcgtttgta tattcggaga tattgtcagg
     2881 cgccggcaat tccatccgcg tgcagcgcgt tcctgcctgc gccggcaaac gattcggtga
     2941 tatccaggcg ggtttcgctg acgcggtacc gatcggcgtc agctggcaac aggaaaagga
     3001 tggcgttccg cgctacgttg cgggactgaa tccggagccg gattacgtga tcgatgccga
     3061 cgatagcctg gtacttttgt gccggggcaa caaggcttcg tacgatgcaa atgcgaagcc
     3121 ggaaattccg gccgccgcac gttcggaaag ttaccggaag ccgccgctga accggatcct
     3181 cgtgctcggg ttcaacgaca acgtgcagga cattctgatc gaattcgatg gccacgtgtc
     3241 gtcaggcagc cacatcgacc tcgtctcgaa cctcgaggac gaggaggcaa ggcagcgcct
     3301 ggagcagaac ctcacgaagc cgttccggaa tatcgaggtg gcttttcgcc acggcagcac
     3361 ggtgtccgcc gctttcctgc gctcactgga cgttctttcg tacgactgca tcatcacgtt
     3421 ggcggatgag tcgaacgagg atgatcccga tgcgcgaagt atcatgctcc tgctgctgtt
     3481 gagcgatatc ataacgtccg atcccagcag ttcccgcatc aatgtcgttt cagagataca
     3541 cgacccggcg aaccgcgaac tggtcgctcg caccgccgcg caggaagtta tcgtcagccc
     3601 cgagatcatc agcatgcagc tgagccagat ctcgcagcag ccggtgctgg aaagcatcta
     3661 tcaggaactc ctgagcgcag ggggcatcga gatatgtatc aaacccgcaa gccgctacgt
     3721 cgacctgctg aagccttgca gctttatcga tttgcagctt gccgcccagc gcatgatgga
     3781 ggtggtcatc ggcgtgcgca ttcacgatcc ggcggctgtc gcccggcata aagtacaatt
     3841 gaatccgcgg cgcgaaacgc ggtgggagtt cggtccgaat gatagtgtcg tcgtccttgc
     3901 acaggagata tatgattagc cggtgcgttg gtaagccatg acaggtaagg gtgcaggtat
     3961 gggcaagatc cttctcgtcg aagacaacca gatgaaccgg gacatgctga gccggcgctt
     4021 gaggcgcaag ggcttcgaag tattgctcgc gaccgacggc caggagggaa tctcgctggc
     4081 gcgtagcggc gcaccggacc tgatcctcat ggacatgagc ctgccgacga tggatggctg
     4141 gaccgccgct cgcctgctga agagcgactc cgatacccgg agcatcccga tcatcgcgct
     4201 gaccgcccat gcgatgcgcg gggatcgcga caaggcactg gatgccggct gcgacgagta
     4261 cgagacgaag cctgtaaacc tcgcccgcct gctgggcaag atcgagtccc tcaccaggtc
     4321 gccgttgagc ccgtagggct cctacgttcc gacgagcatc cgcacgagat tcggcaggcc
     4381 cgctccctgc atcggccggt cccggccaag atcggtgcag ttcgtgagca gtatctgttt
     4441 taccttgtcc ggttggccga tgaactcgcg ccggatcgag agaaacgccg caacgactcc
     4501 cgaaacgtgg ggcgcggcca tgctggt
//
LOCUS       DASUOM010000600         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1655916,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000600 DASUOM010000000
VERSION     DASUOM010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655916"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFY27_12500"
     CDS             <1..331
                     /locus_tag="VFY27_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5766383.1"
                     /translation="GLQQQLFRLLSQDINKAALLAGNYSADERMAAFLISLSRRYATR
                     GFSPNRFTLTMTRTDVANYLRLASESVSRGFRRLQDDGLIRVDRRDIELIDRPALESR
                     ARTILRD"
     gene            586..747
                     /locus_tag="VFY27_12505"
     CDS             586..747
                     /locus_tag="VFY27_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766384.1"
                     /translation="MNAEEKNEESLKVPLIQQLLDNPFLLLFLGVLIPMVVYVLWGVI
                     DILTIPLAP"
     gene            791..1330
                     /locus_tag="VFY27_12510"
     CDS             791..1330
                     /locus_tag="VFY27_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766385.1"
                     /translation="MSAILPPAERLWWKHPLDRVEGTWIVIAFVWCMILFFMMVFWHI
                     YGDQNLSTETYKTTPAQFMAKTQAMVDQYTVRMDAQQTPVVAPPPGSDVYLIARLWSF
                     WPIIEFEKDQTYRLHLTSMDYNHGFSLQPSNINIQMVPGYEHVITVTPNQGGVFSVVC
                     NEFCGIGHHLMVGRIFVRE"
     gene            1344..3044
                     /locus_tag="VFY27_12515"
     CDS             1344..3044
                     /locus_tag="VFY27_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5766386.1"
                     /translation="MSVATTYRICPRSGLQFESRAEKLILANAVAGVVFLLIGGLLAI
                     GVVLTRWPAVHWLEAATFYQVLTAHGLDMLIFWIIFFEIAVLYFCSSTLLRSRIATPR
                     VAWVAFALMIIGAVMNNIAVYQGGSSVMMTSYVPMMAVPSFYLGLILFAVGALIACFV
                     FFGTLVVAKREQTYEGSVPLVTFGAIVAAIIAVFTIVSGAIILIPTYLMSVGLVEHVD
                     PLIYRTIWWAFGHSSQQVNVAAHISVWYLVAAVVFGARPMSERVSRGAFLLYILFLQL
                     ASAHHLLADPGMSTEWKVVNTSYFMYFAVLASMIHGLTIPGAMEVAQRQKGFTRSMFE
                     WLRKAPWGNPVFSGVFISLIGFGFLGGISGVMMGTEQLNMIIHNTIYVPGHFHATVVI
                     GTTLTFMALTYFLIPVLFRREMIAPRLAKWQPYLFGFSMYFFCLMMMGAGTLGVPRRH
                     WDLSFSGMPMAYEWPGAAYLMMGLVGIGGLSAIAGGAIYIYVTVGSLLWGKRLDAGAE
                     RREVTPIPPTGPAVAVQSYGSMGFAAPGTFTLAMVFLGAFVLYYFINWKYLSTLWGLS
                     "
     gene            3123..>3585
                     /locus_tag="VFY27_12520"
     CDS             3123..>3585
                     /locus_tag="VFY27_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX5766387.1"
                     /translation="MTTALAMHDGAARQGIRDVVSLFKLRIGVLIMITALVGLAVSAG
                     PAGFLQIITVAITVLVASASAGAFNQYYECESDALMARTSRRAFVTGALVRTPSWLLL
                     MGMMLAGSVALAAFVLNPVSALYVFLGAFFYAIVYTVWLKRRTWLNIVIGGL"
BASE COUNT          632 a         1108 c         1062 g          783 t
ORIGIN      
        1 tggcttgcag caacagttgt ttcgcctgct gagccaggat atcaacaagg ccgcactgct
       61 cgccggcaac tactcggccg acgagcgcat ggccgctttc cttatttcgc tctcgcgccg
      121 ctacgccaca cgcggcttct cgccgaaccg ctttaccctc actatgaccc gcacggacgt
      181 tgccaactac ctgcgcctcg cgtcggaaag cgtcagccgc gggttcaggc gcctgcagga
      241 cgacgggctg attcgtgtcg accgccgaga catcgagctg atcgatcgcc cggcgctgga
      301 gtccagggcc agaaccatct tgcgcgactg agccgtcgct gcaggagccg gccgtgcggg
      361 ctggttcgct aacgtctgcc gaactcgccc gcgcggcggg ctcctgcaca gatctgcagc
      421 accctctccg ctgcccctat tgcgcgaccc gcagccacgc cgtaaccggc tcacccggcg
      481 agaaatttca actgtgcttg atctggatca gggaaattac cggcgctgag gcttcaaatt
      541 aaggcattgc acgaaatacg ttcgccaggg gatggaggag gccgtatgaa tgccgaagaa
      601 aaaaacgaag aaagcctgaa agtcccgttg atccaacaac tgctggacaa cccgttcctg
      661 ctcttgttcc tgggcgtgct tattccaatg gtcgtttacg tcctctgggg cgtcatcgac
      721 atactgacga taccactggc gccctgaccg ccaacgtgcg atctcaccgg gagaaaaggg
      781 gacaacctgc atgagcgcaa tcctgccacc ggcggaacgg ttgtggtgga agcacccgct
      841 cgatcgggtt gaaggcacct ggatcgtcat cgccttcgtg tggtgcatga tcctcttctt
      901 catgatggtg ttctggcata tctacggcga ccagaacctg tcgacagaaa cctacaagac
      961 gactcccgcc cagttcatgg cgaaaaccca ggcgatggtc gaccagtaca cggtgcgaat
     1021 ggacgcgcag cagactccgg tcgtggctcc gccgccgggc agcgacgtgt acctgattgc
     1081 gcgcctctgg agtttctggc caatcatcga gttcgagaaa gaccagacat accggctgca
     1141 cctgacgtcc atggattaca accatggctt ctccctgcaa ccgagcaaca tcaacatcca
     1201 gatggtgccc ggctacgaac acgtcatcac cgtcacgccc aaccagggcg gcgtattttc
     1261 ggtggtatgc aacgaattct gcggtatcgg ccaccacctg atggttggtc gaatcttcgt
     1321 acgcgaataa tggggagacg cgcatgtctg tagcaacgac ctaccgcatc tgtccgcgct
     1381 ccgggttgca gttcgaatcg agggcggaaa agctgatcct ggccaacgcg gtggcgggcg
     1441 tcgtgttcct gcttatcggt ggacttctcg cgatcggagt cgtgctgaca cgctggcctg
     1501 cggtgcactg gctcgaagca gcaacgttct accaagtact cacggcgcac ggtctggaca
     1561 tgctcatttt ctggatcatc tttttcgaga tcgcggtgct gtatttctgc agctcgacac
     1621 tgttgcgatc ccgcattgcg acgccccggg tggcgtgggt cgcgttcgct cttatgatca
     1681 tcggcgcggt gatgaacaac atcgccgtct accagggcgg ctcgagcgtc atgatgacgt
     1741 cctacgtgcc catgatggcg gttccatcgt tttacctggg cctcatcctg ttcgcggtcg
     1801 gcgcgctgat cgcctgcttc gtttttttcg ggacactggt ggtcgcaaaa cgcgagcaga
     1861 cctacgaggg gtctgtgcct ctcgtgacat tcggtgcgat cgtcgccgcc atcatcgcgg
     1921 tgttcaccat cgtgtcaggc gccatcatcc tgatccccac gtacctgatg tcggtaggcc
     1981 ttgttgagca cgttgatccc ctgatatacc gcacgatctg gtgggccttc ggccactcct
     2041 cgcagcaggt caatgttgcc gcgcatattt cggtctggta cctggtcgct gccgtggtct
     2101 tcggcgcccg gccgatgtcc gagcgtgtca gcaggggagc attcctgctc tacatcctgt
     2161 tcctgcaact ggcgagcgca caccacctgc tcgccgaccc cggcatgagc acggagtgga
     2221 aggtcgtcaa tacaagctac ttcatgtact tcgcggtgct ggcttcgatg atccacggcc
     2281 tgaccatccc gggagcgatg gaggttgcgc agcggcagaa ggggttcacc aggagcatgt
     2341 tcgaatggtt gcgcaaggcc ccctggggta acccggtttt ctcgggcgta ttcatttcgc
     2401 tcatcgggtt cggtttcctc ggtggcatct ccggcgtgat gatggggacc gaacagctca
     2461 acatgatcat ccacaacacg atctatgtgc cgggtcattt ccacgccacc gttgtcatcg
     2521 gcacgacatt gactttcatg gcgctgacct atttcctgat ccccgtgctg ttcaggcgcg
     2581 agatgatcgc gccgcgcctc gcgaagtggc agccctacct gttcggcttc tcgatgtact
     2641 tcttctgtct catgatgatg ggtgcaggca ccctgggcgt cccgcgccgt cactgggact
     2701 tgtcctttag cggtatgccc atggcctacg agtggccggg agctgcctac ctgatgatgg
     2761 gcctggtcgg gattggcggc ctctcggcaa ttgcgggggg tgcaatctac atctacgtta
     2821 cggtcggctc cctcctttgg ggcaaaagac tggatgccgg tgcggagcgc agggaggtga
     2881 ctccgattcc gccgacgggg ccggccgtgg ccgtgcaaag ctacggatcg atgggtttcg
     2941 ccgcgccggg tacgttcacg ctggcaatgg tctttctcgg cgccttcgtc ctttattact
     3001 tcatcaactg gaagtatctc tcgacgcttt ggggcctcag ctgatgctgc tgctgaccga
     3061 cggctgcggc aaccggtagt tgcttgaggc tgccacgtaa ctttgacgat caggaggccg
     3121 ccatgacgac ggcactggca atgcacgacg gagccgcgag gcaaggaatc cgcgacgtag
     3181 taagcctctt caagctgcgc atcggcgtcc tgatcatgat taccgcgctg gtcggcctcg
     3241 cggtgtcggc aggccccgcc ggtttcctgc agatcatcac cgtcgccatc accgtgctgg
     3301 tcgcctccgc gagcgcgggc gcgttcaacc agtactacga gtgcgagagc gacgcgctga
     3361 tggcccgcac gagccgtcgc gccttcgtca ccggggcgct ggtccgaacg ccttcctggc
     3421 tgctgctgat ggggatgatg ctggccggct ccgtcgctct tgcagcgttt gtcctgaatc
     3481 cggtcagcgc actttacgta ttcctcggcg cattcttcta cgcgatcgtc tacaccgtat
     3541 ggctgaagcg gcgtacctgg ctcaacatcg taatcggagg actgg
//
LOCUS       DASUOM010000601         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_594554,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000601 DASUOM010000000
VERSION     DASUOM010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594554"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFY27_12525"
     CDS             complement(<1..249)
                     /locus_tag="VFY27_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5766388.1"
                     /translation="MPKNAIQLLKEDHKKVKDLLSQLVETTPRAEKTRRQLLEKIAQE
                     LEIHTRIEEEIFYPAFKNAGNGEHAKMYFEALEEHRAVD"
     gene            486..1220
                     /locus_tag="VFY27_12530"
     CDS             486..1220
                     /locus_tag="VFY27_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5766389.1"
                     /translation="MAIAAWKRLERTPAYQRKKLFFKRLVGRELRLRPEVDVHSVKDG
                     GWWFHPDALGEDSIAWSLGIGEDVDFDLALIRRFHLRVDAFDPTPSTVEWLAAREMPE
                     SFHFHPWAVTAADGFLKLYPRVRRDGSKSTVMYSMVAGEPSRDDAIVVPAFCLQSIQA
                     ELGQQRLDLLKMDIEGAEYDVLDSLLESSVRPLQLLVEFHHRFAGVGLARTEEMIAKL
                     RSRGYRIFAVSETGREVSFILSAEVG"
     gene            1223..1456
                     /locus_tag="VFY27_12535"
     CDS             1223..1456
                     /locus_tag="VFY27_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766390.1"
                     /translation="MRASLYAICGIAAGVLVGASSAGCAGKAGYSPPPERRAGERCPV
                     GETLVCRDQYPSRLPDREDDPLICYCDNIPVTR"
     gene            complement(1570..3210)
                     /gene="groL"
                     /locus_tag="VFY27_12540"
     CDS             complement(1570..3210)
                     /gene="groL"
                     /locus_tag="VFY27_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145211.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5766391.1"
                     /translation="MAAKEVRFSDDARQRMFAGVNILANAVKVTLGPKGRNVVLDKSF
                     GAPTVTKDGVSVAKEIELDDKFENMGAQMVKEVASQTSDIAGDGTTTATVLAQAILRE
                     GLKSVAAGMNPMDLKRGIDKASIAIVKELEKLSKPCADDKAIAQVGSISANSDPEIGK
                     IISEAMQKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNQQSQSVELENP
                     LVLLFDKKISNIRDMLPVLEGVAKAGRPLLIIAEDVEGEALATLVVNNIRGIVKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGQVVSEEVGLSLEKTTLDDLGEAKKVQITKENTTIID
                     GAGRAEDIKGRVDQINAEIEESSSDYDKEKLQERVAKLSGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVAFIRAVAAISKLKGDNDDQTVGINILRRAVEEP
                     LRQIVKNAGGDASVVLNAVAEGKGNFGYNAATGEYGDMIELGILDPTKVTRYALQNAA
                     SVSGLLLTTEAMVAEAPKEESGHAMPDMGGMGGMGGMM"
     gene            complement(3279..3569)
                     /gene="groES"
                     /locus_tag="VFY27_12545"
     CDS             complement(3279..3569)
                     /gene="groES"
                     /locus_tag="VFY27_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5766392.1"
                     /translation="MKIRPLHDRVIVKRMEEERTSPGGIVIPDTAAEKPIKGEVIAVG
                     NGKILENGEIRALDIKAGDKVLFGKYSGTEVKVDGEELLVMKEDDIMAVIES"
BASE COUNT          713 a         1203 c         1142 g          912 t
ORIGIN      
        1 atccacggca cgatgttctt cgagagcttc gaagtacatc tttgcgtgct ctccgttacc
       61 ggcattcttg aaggccggat aaaaaatttc ctcttcgata cgggtgtgga tttccagctc
      121 ctgggcgatc ttctccagca gctggcgccg cgttttttcc gctcgcggcg tagtttcgac
      181 gagttggctc aggagatcct tcaccttctt gtgatcttcc ttcagtaact ggatcgcgtt
      241 ctttggcatg gcggtgtctc ccttcgcggt tgtttatcgt tcagcattgc agcaagaaat
      301 gtgccacggg tagcagtccc ggcgccctca ccaatccgcc actcggtatg gaaggattta
      361 ccgcctgctt tgcagttcct acgtgagtag ccgcattgcg tgagtctaat gccggtagcg
      421 aaggtgctaa gcttggtgcc agcgtcgacc gaccaaccat tggtcccggg cacctggatt
      481 cagtgatggc cattgcagcg tggaaacgcc tcgagagaac tcccgcctac cagcgcaaaa
      541 agctgttctt caagcggctg gtcggcaggg agcttcgcct caggccggaa gtcgacgtcc
      601 actccgtcaa ggatggcggc tggtggttcc accccgatgc gctgggggaa gacagcattg
      661 cctggtccct ggggataggg gaagacgtcg acttcgacct cgccctgata cggcgtttcc
      721 acttgcgggt ggacgcgttc gatccgaccc cttcaacagt cgagtggctt gccgcgcggg
      781 aaatgccgga aagcttccat tttcacccgt gggccgttac ggcagcggac ggtttcctga
      841 aactttaccc gcgcgtcagg agggacggga gcaagtccac ggtcatgtac tcgatggttg
      901 cgggcgaacc gtcacgcgac gacgccatcg tagtgcccgc gttctgcctc cagagcatcc
      961 aggcagaact ggggcagcag cgtctcgacc tgctgaaaat ggacatagag ggcgccgagt
     1021 acgacgtcct cgatagtttg ctcgaatctt cggtcagacc gctgcaattg ctggtggaat
     1081 tccatcatcg ctttgccggc gtcgggctcg ccaggacaga agagatgatt gcgaagcttc
     1141 gaagccgcgg gtacaggatc tttgccgttt ccgaaacagg acgggaagtt tcgttcattc
     1201 tttccgccga agtgggatag tcatgcgggc gagtctgtat gcgatctgcg gcattgccgc
     1261 cggcgtgctc gttggcgcca gtagcgccgg ttgtgccgga aaggctggct actcaccgcc
     1321 gccggagcgg cgggccggcg agcgatgccc ggttggtgaa actctggtgt gcagggatca
     1381 atatccctcg cgcttgccgg accgggaaga cgatcccttg atctgctact gcgacaacat
     1441 tcccgtcacg cgctaggctc gcaggagcct gttgtgcagg cgaccttcaa ccgcactcgt
     1501 cggcatggta atgctcctca aaaaaagccc cgcatatgcg gggctttcgt gttgcgggaa
     1561 cgcactgctt tacatcatgc cgcccattcc gcccatgccg cccatgtcgg gcatagcgtg
     1621 gccggattct tccttcggtg cttcggccac cattgcttcg gtggtcagca gcaggccgga
     1681 gacggacgca gcattctgca gcgcatagcg ggtgaccttc gtcgggtcga gaataccgag
     1741 ctcgatcatg tcgccgtatt cgccggtagc cgcgttgtag ccgaagttgc ccttgccctc
     1801 tgcaacggcg ttcaacacga cgctggcatc gccgccggca ttcttcacga tctggcgcaa
     1861 cggctcttcg accgcccgcc ggaggatgtt gataccgaca gtctggtcgt cgttgtcacc
     1921 cttcagcttc gagatggcgg caacggcgcg gatgaatgca acaccaccac ccggtactac
     1981 gccttcttct acggcggcgc gggtagcgtg cagcgcgtct tcgacacgtg ccttcttctc
     2041 tttcatctcg acttcggttg cggcaccgac cttgatcacg gccacaccgc cggacagctt
     2101 ggcaacgcgc tcctgcagct tttccttgtc gtagtcggaa ctcgactcct cgatctcggc
     2161 gttgatctgg tcaacacggc ccttgatgtc ttcggcacgg ccggcgccat cgatgatggt
     2221 cgtgttttcc ttggtgatct gcaccttctt cgcttcgccc aggtcatcaa gcgtcgtctt
     2281 ctcgagggac agtccgactt cctcggaaac aacctgccca ccggtcagaa cggcgatgtc
     2341 ctgcagcatt gccttccggc gatcgccgaa accgggcgcc ttgacggcgg caactttcac
     2401 gataccgcga atgttgttca ccaccagggt agcgagagcc tcgccttcta cgtcctctgc
     2461 gatgatcagg agcggacggc cagccttggc cacgccttcc agtaccggca gcatgtcgcg
     2521 gatgttcgag atcttcttgt cgaacaacag caccagcgga ttctcgagct ctacgctctg
     2581 gctttgctgg ttgttgatga agtaggggga gaggtaaccg cggtcgaact gcatgccctc
     2641 gacgacgtcc agctcgttct cgagtccgga accttcctca acggtgatga cgccttcctt
     2701 gcccactttc tgcatcgcct cggaaatgat cttgccgatt tcagggtccg agttggcgga
     2761 gatgctgcct acctgtgcga tcgccttgtc gtctgcacag ggcttggaca gcttctcgag
     2821 ttccttgacg atcgcgatgc tggccttgtc gatgccgcgc ttcaggtcca tcgggttcat
     2881 gcctgcggca accgacttca ggccttcgcg aagaatagcc tgtgccagta cggtcgcggt
     2941 ggtggtgccg tcaccggcga tatcggaagt ctgcgatgca acttccttga ccatctgggc
     3001 acccatgttc tcgaacttgt cgtcgagctc gatttccttg gcgacagaca cgccgtcctt
     3061 ggtgacggtc ggtgcgccga agctcttgtc gagtaccacg ttccggccct tcgggcccag
     3121 ggttaccttt accgcattag ccaggatgtt tacgcctgca aacattctct ggcgtgcgtc
     3181 gtcgctaaaa cgaacttctt tagcagccat ttaacttatc ctctggaaaa tagtgatgtc
     3241 gtaaaatcag gtctcgagct tggaaccagt cccggctcct agctctcgat aaccgccatg
     3301 atgtcgtctt ccttcatcac gagcagttcc tcaccgtcga ccttgacctc agtgccgctg
     3361 tacttgccga acagcacctt gtcgccggcc ttgatgtcca gtgcacgtat ttcaccgttc
     3421 tcgagaatct tgccattgcc aactgcaatg acttcgccct tgatcggctt ttcggcggcc
     3481 gtgtcgggaa tgacgattcc gccgggggac gtgcgctctt cttccatgcg cttgacgatg
     3541 acgcgatcat gaagcggacg aatcttcatc gattagtact cctctaaact tttgattttt
     3601 aacgactgtt gagtaaactg ggggtcgcgg cctgttagca ctcgacctca gggagtgcta
     3661 atgataggga cggcgcttca aatttcaagg ggcccggtga aaggagcggc caccggatgc
     3721 gaggtcacct gccgggtcca ggcaaggatt cgcggcctgc gtccgggcat cccggaacgc
     3781 ggcgcccgta tcctccgcgg aagaaaacca cagagaggcg ctgcggcagc ataaccggta
     3841 acatctggcg ccttgaactg cgcttggttg gctaccgccc ttattcatgg cgccagccag
     3901 ggctgacgcc cgcaccgccg aggccgactg cggtcaaacg acgacattat gaacagattc
     3961 ccggtacatc
//
LOCUS       DASUOM010000602         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_868765,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000602 DASUOM010000000
VERSION     DASUOM010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868765"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..651)
                     /locus_tag="VFY27_12550"
     CDS             complement(277..651)
                     /locus_tag="VFY27_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5766393.1"
                     /translation="MRLILDTHLLLWALGQPARLSAAARKQIEKADVYISAASIWEIS
                     IKATLGKLKANPAEVLGAIEPAGFILLDITGEHAAKTIELPPHHKDPFDRMLVAQASY
                     EPMILLTNDAALADYGSFVKLI"
     gene            complement(654..893)
                     /locus_tag="VFY27_12555"
     CDS             complement(654..893)
                     /locus_tag="VFY27_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX5766394.1"
                     /translation="MGNVINIHEAKTHLSKIVDDVAAGAEVIIAKAGKPMARLSPINP
                     PARRKKLGLLKGKIKVPDDFNTPLDDEVIAAFEDR"
     gene            1565..2773
                     /locus_tag="VFY27_12560"
     CDS             1565..2773
                     /locus_tag="VFY27_12560"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5766395.1"
                     /translation="MAGIFAVTGAAWVLLTDIVLYGLTRDPVLVARIETAKGWAFVVL
                     ATVLLYLVTFRSVRRFVRAKAGITAVVDSIADGVLLLGPDRTIRRANPAAIRMLGEDL
                     VGMDAEQFSRRFRVSYLDGSIVPPREFVSQRVFDEGGPLIYKAILHPDNGTERIVSVT
                     AAAVRVYADEPPDMVVSVMHDITESERRNALRDQFFAAAAHALKTPVAIIKANAELLL
                     TDGGGPGREAAVAIGQQCDRMDLLVQNLFVVGRIRSRSLKLYPQEVAIAPLVEQVMTS
                     VAARSPGYSFNSEFNVRPQPRVHVDQERLALVLRNLLDEAMQLSAPSEPISVLMAHDG
                     VDAEIGVRHDSRATSPEPESSGGEYDELGINRLVTSTIIVAHGGDLRRDTEDGMTTAW
                     IRLPMIEARG"
     gene            2770..3153
                     /locus_tag="VFY27_12565"
     CDS             2770..3153
                     /locus_tag="VFY27_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766396.1"
                     /translation="MTGGSDILVVEDEAAMADAMLRMLSITGYAARRAENGLQALKAV
                     AKQRPALVLLDILMPVMDGWQCARELRSRYGDSLPIVIVTAAEHVRARAAALSADDVL
                     AKPFEMQDFLAVVARYAAHCRAKTD"
     gene            3251..4363
                     /locus_tag="VFY27_12570"
     CDS             3251..4363
                     /locus_tag="VFY27_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766397.1"
                     /translation="MSNRGKASLERGNTDEGLRRERDKADRALEKRNDDAEEDADRIV
                     RHARETADEVLSAARDEADDKLDRAAPSVPSRAGVAGERVREDRAVDEERSAADAVLS
                     REREETARVLMRLLPLERESTDRYLLTERERSDEALSNRDDFLGIVSHDLRDLIGGIV
                     MSASVLAATAPTNDEGRNTLIYTDRIHRYAARSNKLIGDLLDVASIEAGKLAVVPARA
                     DAALLISEAVETFFDSASAKGISLLAGDVDRPLVCEFDYPRLLQVFVNLISNAIKYTP
                     EGGRISVDGKRTGTDVHFSVTDSGSGIPGHLLEAIFQRFWQVDGEKSRGIGLGLYICR
                     SIVKAHGGRIWVESEQGVGSAVHISLPDPSQRPERH"
BASE COUNT          835 a         1308 c         1409 g          849 t
ORIGIN      
        1 cggccgacag caggaccgag atcaccgcga cgataaggtt ttgcatgtca ttctcctcac
       61 gagctattca atacccggtg ttatggctca attggtgagc agcacgaccg attaagtact
      121 ccacgatatt caggacgagc gtcgtttacc gacgctgatc agagagggag ggccgccagg
      181 caaccgcgct tgaatcaggc ggcggaccga ggagcggctc gcctaccgcc gggtcattgc
      241 ccagctgcag actattcgtc tttgacggcg tcttcctcaa atcaatttaa cgaatgatcc
      301 gtagtcggcc aaagctgcgt cgttggtgag caggatcatc ggttcgtagg acgcctgggc
      361 gacgagcatc cggtcaaacg ggtctttgtg gtgcggcggt agctcgatcg tcttggctgc
      421 atgctcaccg gtaatgtcca aaagtatgaa acccgcaggc tcaatggcac cgagtacctc
      481 ggcgggattt gctttaagtt tgccgagcgt cgccttgata ctgatctccc aaatggaggc
      541 cgcactaata taaacatctg ccttttcgat ttgcttgcgt gccgctgcag aaagcctggc
      601 gggctggccg agtgcccaca gcaacaagtg cgtgtccagg atcagccgca cgatcagcga
      661 tcttcaaaag ccgcgataac ctcgtcatcg agcggcgtat tgaagtcatc gggaaccttg
      721 atcttgcctt tcaggagccc cagctttttc cgccgtgcgg gggggttgat ggggctgagc
      781 cgcgccattg gtttacccgc cttggcgatg atgacctcgg caccggcggc aacgtcgtcc
      841 acgatctttg acagatgcgt cttcgcctcg tggatgttga tgacattgcc catcgcctac
      901 gtccggaagt aaactagact tagtttaact tgggatcaca cagccggcaa gtatccaagg
      961 cgcgtcgcgg cccctcatga aaatgacggg caagtcactg ccgcttgcca caagatatct
     1021 ctggcggtca aagcccgaca tgccatcgat gtgtatgtcc agcaacaggc aatcaaccag
     1081 gtctgacttc gcacgctcga gaaactcctc cgcagaggca cagccacgcg tccggaaccg
     1141 ctcccagcaa ctgacccaga gcacaacgga cactttcgtc gtcgtcatcg atgccggtga
     1201 tccggtagtc acgcggagca cccgcacttg tgcacacagt ccgggccatg attgtgaaac
     1261 cacggcgaaa caggcaagcc gtcgattgcc cgttagggca aggtcgttct cgtcgagctc
     1321 gcgagcattt cgttcgcgtc ggctattggt atcgccgcgc gccgaaagtt gatcccgggc
     1381 agcgagcgat cacagtgctc gggagccccg gcagcccggt gctataattc gatctccgcc
     1441 cgctgcgcgc atctagacag tgccccacag aacgcgtgca gccggtatgc attccgggtg
     1501 tacgcgcgtg tggtctgtct tcgccaaagg gaagcatccc tccgctcata ggatcgcgct
     1561 tgccgtggcc ggcatcttcg cggtgactgg cgcggcgtgg gtgttgctca cggacatcgt
     1621 gctctatggc ctgacacggg acccggttct tgtggcgcgc atcgagaccg ccaagggctg
     1681 ggcgtttgtc gtgctcgcca ccgtgttgct ctacctggtg actttccgga gcgtgcgtcg
     1741 ctttgtacgg gccaaggccg gaatcacggc ggtcgtcgac agcattgccg atggggtgct
     1801 cctcctcggt cccgacagaa ctattcggcg cgcaaaccct gcggcgatcc ggatgcttgg
     1861 cgaggatctc gtgggcatgg atgcggagca gttttcccgc cgattccgcg tttcctatct
     1921 cgacggttcc atcgtgccgc cgcgggagtt tgtatcccag cgggtgttcg acgaaggagg
     1981 accgctgatt tacaaggcca tcctgcatcc cgacaacggg accgagcgca ttgtctcggt
     2041 aacggcagcg gctgttcggg tgtatgccga cgagccaccc gacatggtcg tgagcgtgat
     2101 gcacgacatt accgagtcgg aaaggcggaa cgcgcttcgc gaccagttct tcgcggcagc
     2161 ggcgcatgcc ctcaagacac ccgtagctat aatcaaggcc aacgccgagt tgctcctcac
     2221 cgacggcggc ggacccggga gagaagcagc tgtcgccatc gggcagcagt gcgaccgcat
     2281 ggacctgctg gtccagaacc tctttgtagt gggccggatc cggtcgcgca gtctgaagct
     2341 ctatccgcag gaagtggcga ttgcgccgct ggtcgaacag gtcatgacct ccgttgccgc
     2401 acggtcacca gggtactcct tcaacagtga gtttaacgtg cgcccgcagc cacgcgttca
     2461 cgtcgaccag gaacgcctcg cgctcgtcct tcgcaacctc ctggacgaag cgatgcagct
     2521 ctccgcaccg tcggaaccga tcagcgtgct gatggcacac gacggtgttg acgcggagat
     2581 cggtgtccgg cacgactcgc gtgcgacgtc gccggagccg gagtcctccg gtggagagta
     2641 tgacgagctc ggaatcaacc gcctggtcac cagcacgatc atcgtggcac acggcggaga
     2701 tctgcgtcgt gacacggagg acgggatgac gacggcgtgg atcaggctac caatgatcga
     2761 ggctcgcggg tgaccggagg gtctgacatt ctcgttgttg aggacgaggc cgccatggcg
     2821 gacgccatgc tccgcatgct cagcatcacg ggctacgcag cgcgccgtgc ggagaacggg
     2881 ctgcaagccc tgaaggcggt cgcgaaacag aggcccgcgc tcgtgctgct cgacatcctg
     2941 atgccggtta tggacggctg gcagtgcgcc agagagttgc gcagccggta tggggattcg
     3001 ctgccgatcg tcatcgtcac cgcggcggag cacgtgcggg cgcgcgccgc cgcgctgagc
     3061 gccgacgacg tcctggcgaa gcccttcgag atgcaggatt tcctggcagt ggttgcgcgc
     3121 tacgctgcgc attgtcgagc gaagaccgac tgagacctct gcacagtacc catcgaagcg
     3181 gctgtaggag cccgcagcga caacgcagat cgcgggcgta ccagcagatg cctgtgatag
     3241 tctttccagg atgagtaata ggggtaaagc gtcgcttgag cgcggaaaca ccgacgaggg
     3301 gctgcgccgc gaacgtgaca aagccgatcg tgcgctcgag aagcgcaatg atgacgccga
     3361 agaggacgcc gatcggatcg tccggcacgc tcgtgagact gccgacgagg tgctgagtgc
     3421 ggcgagggac gaggcagacg acaagctcga tcgagcggct ccctccgttc cgtcgcgggc
     3481 cggcgtcgcg ggcgaacgag tgcgcgaaga cagagccgtc gacgaggagc gtagtgccgc
     3541 cgatgcggtt ctctcccgtg aacgcgagga gacggcgcgg gtattgatgc gtcttttgcc
     3601 gctagagcgt gagtccacgg atcgatacct gctgacggag cgcgagcgtt ccgacgaggc
     3661 gttgtcgaat cgggatgact ttctgggcat cgtgagccat gacctgcgcg acctcatcgg
     3721 gggcatcgtc atgagcgctt cggtgctcgc tgcgacggcg cccacgaacg acgagggcag
     3781 gaatacgctc atatataccg accgcattca tcggtacgcg gcccgctcga acaagctgat
     3841 cggcgatctg ctcgacgtcg ccagcatcga ggccggcaag ctcgctgttg ttccggcccg
     3901 cgcggatgcg gcattactga tttcggaagc ggtggagact tttttcgact ctgcatcggc
     3961 gaagggcatt tccctcctgg cgggcgatgt cgatcgaccg ctggtctgcg agttcgatta
     4021 cccgcgtctg ttgcaggtgt tcgtgaattt gatctcgaac gccattaagt acacgccgga
     4081 aggggggcgc atcagcgtcg atgggaagcg caccggcacg gacgttcatt tttccgttac
     4141 ggacagcggg tcgggcattc ccggacacct gctggaggcg atcttccagc gcttctggca
     4201 agtggacggc gagaagtcga gaggaatcgg cctcggcctc tacatctgta gatcgattgt
     4261 caaggctcac ggcggccgga tctgggtaga gagcgagcaa ggcgtcggca gcgctgttca
     4321 tatttcgctt ccggatccat cgcagcgtcc ggagcggcat tagcccggga ttgacgggtt
     4381 cgaggtcgcg cgtcggctga g
//
LOCUS       DASUOM010000603         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_525987,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000603 DASUOM010000000
VERSION     DASUOM010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525987"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="VFY27_12575"
     CDS             <1..1616
                     /locus_tag="VFY27_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5766398.1"
                     /translation="FCAMAVIAWCPGVASQEDENREALRMAVERLRYNVDGANTAADA
                     NASRLVALFYERRNFFAAWEDSWKIRSLITVVEDSYKDGLSPTDYHLSDLTEQYARAR
                     AADLRSPEEWAAFELDLTDALISLVHHQRFGKADPLSQHATWNYKEELHESLVLEIAE
                     KAVSARSPGESLEESIPRGPYYRRLRGALASYRDIASSGGWPSIEDGPSLDLGLSDER
                     VPVLAARLSATGDLDDASPYFESKTVDGRLQEAIRRFQERHSLEADGIAGPATIRALN
                     VPVEERVAQLRLTLERARWVLGGLGRDFVLVNIAGFRAYVIDDGQVAWETRVVIGKAQ
                     QQTPVFRDELQYIVFNPTWTVPYSIATREMLPEIKLQPDWFAKHDFDLKDSDGEPMDP
                     STVDWASVTRQNFNYIFVQRPGPNNALGEVKFMFPNEHAVYLHDTPARQLFASSDRAF
                     SHGCIRVEHPLELAEILLAANGWDRGRIDAAIASGETTTVFLAKPLPILLLYWTANVT
                     PDGTVHFYRDVYRRDAAIAQALDGPFSIP"
     gene            complement(1625..4198)
                     /gene="mutS"
                     /locus_tag="VFY27_12580"
     CDS             complement(1625..4198)
                     /gene="mutS"
                     /locus_tag="VFY27_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5766399.1"
                     /translation="MSSSVASVHTPMMQQYLRIKGEYPDMLVFYRMGDFYELFFDDAK
                     RAAALLDITLTSRGKSGGAPIPMAGVPYHAAEGYLAKLVRKGESVAICEQIGDPAISK
                     GPVERRVTRIVTPGTLTDEALLTETRDNLVAAVFAGNGGETIGVAWLDLSGGRFRLTE
                     LGGLDALAGELERLRPAEIIYNEEQQPSIGSLNGKTRATARPPWHFDLDSGTRLLCSQ
                     FGTKDLGGFGCEDMRFGIAAAGALLQYVSDTQKASLPHILSIVAERTADAVIMDAPTR
                     RNLELEESLGGRREHTLAGVMDRCQSPMGSRLLRRWIQRPLRDKETLRSRYQAVEAIM
                     LGGCAGKIRELLHGIGDIERILARVALRSARPRDLRHLLLALTRLPQLKKLLQQADSP
                     LLRSLDVQLDPHPPQREMLKKALVDNPPMLIRDGGVIAEGFDEELDELRDIARNADRY
                     LVELEAREKQRTGIPTLKVGYNRVHGYYLEISKGHAHRAPQDYTRRQTLKGAERYVTP
                     ELKSFEDKVLSANERSLSRERFLYERLLDDLLEVLRSLQSTAAALAEIDVLHCFAERA
                     EALNLSAPRIVDEPCLEIAGGRHLVVEQVIETPFVPNDLMLSDNRRLLVITGPNMGGK
                     STYMRQAALIVILAHIGSFVPADSARIGPVDRIFTRIGASDDLAGGRSTFMVEMTEAA
                     SILHNATSESLVLMDEIGRGTSTFDGLSLAWAVAHHMCEQVRAFTLFATHYFELTALA
                     AEIPDCVNVHLDATEHQGKLVFMHAVKPGPANQSYGLQVAALAGVPRNVIRRAKQYLR
                     VLESQQPARQEHPQTQLPFEVADEARDALREAVDAVDPDVLTPREALDLVYRLKGLSG
                     R"
BASE COUNT          774 a         1356 c         1344 g          808 t
ORIGIN      
        1 cattttgcgc aatggccgtc atcgcctggt gccctggcgt agcctcacag gaggatgaga
       61 accgcgaagc gctgcggatg gcggtggagc ggctccgtta caacgtcgac ggggccaaca
      121 ccgcggcaga tgccaacgcc agccgcctcg tcgctttgtt ctacgagagg cgcaatttct
      181 tcgcagcatg ggaggacagt tggaagatac gatcactgat cacggttgtc gaagactcgt
      241 acaaggacgg cctctcgcca acggactacc acctttcgga tctcacggag cagtatgcgc
      301 gcgcccgggc tgcagacctg cggagcccgg aggaatgggc cgcgttcgaa ctggatttga
      361 cggacgcgct gatcagcctc gtgcatcatc aacgcttcgg caaagcagat ccgctaagcc
      421 agcacgccac ctggaactac aaggaggagc tgcatgagtc gctggtcctc gagattgccg
      481 aaaaggctgt aagcgcgaga tcgccggggg aatccctgga ggaaagcata ccgcgcgggc
      541 cctactacag gcgattgcgc ggcgcgctcg cgagctatcg ggacatcgcc tcctcggggg
      601 gctggccttc cattgaggac ggtccttcgc tcgacctggg cttatcggac gaacgcgtac
      661 cggtcctcgc ggccaggctt tcagctaccg gcgatctcga cgacgcgagt ccctactttg
      721 agagcaaaac ggtggatggc aggctgcagg aggctatccg ccgctttcag gaacgtcatt
      781 cgctggaagc cgacggcatc gccggaccgg cgaccatacg ggcgctaaat gtcccggtag
      841 aggaacgcgt cgcgcagttg cggcttacgc tggaacgcgc gcgttgggtt ctcggaggac
      901 tcggcaggga cttcgtgctc gtcaacatcg ccggattcag ggcttacgtg atcgacgatg
      961 gccaggtcgc atgggagacg cgggttgtaa tcggcaaggc gcagcagcag accccggtgt
     1021 ttcgcgacga gctgcagtac atcgttttca atccgacatg gacagtgccc tacagtatcg
     1081 ccacgcgcga gatgctgccg gagatcaagt tgcagccgga ctggttcgcg aaacacgatt
     1141 tcgatctcaa ggattccgac ggcgaaccga tggacccctc gaccgtcgac tgggcatcgg
     1201 taacccggca gaacttcaac tacatattcg tgcaacggcc cggaccgaac aacgcgctgg
     1261 gagaggtcaa gttcatgttc cccaacgagc atgcggtgta cttgcacgat acgccggcaa
     1321 ggcagctgtt cgcctcgtca gatcgcgcat tcagccacgg ctgcatccgc gtcgagcacc
     1381 cgctcgagct tgcggaaatc ctgcttgctg caaacggatg ggatcgcggc cgcatagatg
     1441 cagcaatcgc gtccggcgag actacgactg tattcctcgc gaagccgtta ccgatcctgc
     1501 tcctctactg gacggctaac gtcacgccgg acggcaccgt acatttctat cgagacgttt
     1561 atcggcgcga tgcggcgatc gcacaagctc tggacggtcc gttcagcatc ccgtgactgc
     1621 gcgatcagcg tccgctgagc ccctttagcc gatacaccag atccagcgct tcgcgcggcg
     1681 tcaggacatc cggatcgacc gcatccactg cttcccgcag tgcatccctt gcctcgtccg
     1741 caacctcgaa cggcagctgc gtttgcgggt gctcctgcct cgccggttgc tgcgactcca
     1801 gcactcgcag gtactgcttc gcgcgccgga tcacgttcct gggcacgcct gccagcgctg
     1861 ctacctgcaa accataactc tggttcgccg gtcccggctt caccgcgtgc atgaatacga
     1921 gtttgccctg gtgctccgtt gcgtcgaggt gcacgttgac gcaatccgga atctccgcgg
     1981 cgagcgccgt cagttcgaaa tagtgcgtgg cgaacagcgt gaacgcgcgc acctgctcac
     2041 acatgtgatg cgccactgcc cacgcgagag agaggccatc gaacgtgctg gtaccgcgtc
     2101 cgatctcgtc catcagcacg agactttcgc tggtggcgtt atgcaggatg gaggcggcct
     2161 ccgtcatctc caccatgaac gtcgagcgcc cgccggcgag gtcatcagat gcgccgatgc
     2221 gggtgaagat ccggtccacc gggccgatcc gcgcactgtc ggccgggacg aagctgccga
     2281 tgtgcgcgag gatcacgatg agcgccgcct gcctcatgta cgttgatttg ccgcccatgt
     2341 tcgggccggt gatgaccagc agccgccggt tgtccgatag catcaggtcg ttcggcacga
     2401 acggcgtctc gataacctgc tcgacgacaa gatgcctgcc gcccgcaatc tcgaggcatg
     2461 gctcatcgac gatccggggc gcgctcaaat tcagcgcttc tgcgcgctcc gcgaaacaat
     2521 gcagcacatc gatttccgcc agtgcggctg cggtactttg cagtgacctg agcacctcga
     2581 gcaggtcatc gagcaaccgt tcatagagga atctttcccg gctgagcgat cgttcgttgg
     2641 cgctcaggac cttgtcttcg aagcttttca gttccggggt aacgtaacgc tcggcgccct
     2701 tcagcgtctg gcgccgcgtg tagtcctggg gcgcgcggtg agcatggcct ttgctgattt
     2761 cgaggtagta cccgtgcaca cggttgtagc cgaccttcag cgtcgggatt cccgtccgct
     2821 gcttttcacg agcttccagt tccaccaggt aacgatcggc gttccgggcg atgtcgcgca
     2881 gctcatccag ctcttcgtcg aagccttccg cgatgacgcc gccgtcgcgg atcagcatcg
     2941 gcggattgtc gaccaatgct tttttcagca tttcgcgctg tggaggatgc gggtcgagtt
     3001 gcacatccag tgaccgaaga agcggcgaat ccgcctgctg cagcaacttc ttcaactgcg
     3061 gaagccgcgt cagcgcgagc aaaagatgac gaagatctcg cggacgcgcc gagcgcaggg
     3121 cgacacgggc gaggatgcgt tcgatatccc cgatcccgtg cagcaactcc cggatctttc
     3181 ccgcgcatcc tcccagcatg atggcttcga ccgcctggta ccgggagcgc agcgtctcct
     3241 tgtcgcggag cggccgctgg atccagcgcc tgagcagccg gctgcccatc gggctctggc
     3301 agcgatccat gacgccggcg agcgtgtgct cgcggcggcc gcccaggctt tcttcgagtt
     3361 cgaggttccg ccgcgtggga gcgtccatga tcacggcgtc ggcagtgcgt tccgcaacga
     3421 tcgacaggat gtgcggcagg ctggcctttt gcgtatcgct gacgtattgc agcagggcgc
     3481 cggccgcagc gatgccgaag cgcatgtcct cgcagccgaa cccgcccagg tctttggtgc
     3541 cgaactgcga gcaaagcagg cgcgtgccgc tgtcgagatc gaaatgccat ggcggacgcg
     3601 ccgtggcacg cgttttcccg ttcagggaac cgatcgaagg ttgctgctcc tcgttataga
     3661 tgatttccgc tggccgcagc cgttcgagct cgccggcgag agcgtcgagg ccgcccagtt
     3721 ccgtcaggcg aaaccggccg ccggacagat ccagccaggc aacgccgatg gtttcgccgc
     3781 cattgcccgc gaacaccgcg gcgacgagat tgtcgcgagt ctccgtcagt agcgcctcgt
     3841 ccgtcagcgt tccgggggtg acaatccggg tgacccgccg ctcgaccggt cccttgctaa
     3901 tcgctggatc gccgatctgc tcgcagattg cgacggattc acctttgcgt accagcttgg
     3961 ccaggtagcc ctccgccgcg tggtaaggga caccggccat cgggatcggc gctccgcccg
     4021 acttgccgcg ggacgtgagc gtaatatcga ggagcgccgc ggcgcgcttt gcatcgtcga
     4081 agaacagctc atagaagtcg cccatgcggt agaacaccag catgtccggg tactcgccct
     4141 tgatgcggag gtattgctgc atcatgggcg tgtggacgga ggcgacgctg gagctcatcg
     4201 gcgtttattc ttcggctggc ggcgagcggt gcatgataac gggagacgcc ctgctgtagg
     4261 agcccgccaa gcgggcgagc ga
//
LOCUS       DASUOM010000604         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1191189,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000604 DASUOM010000000
VERSION     DASUOM010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191189"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFY27_12585"
     CDS             complement(<1..380)
                     /locus_tag="VFY27_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766400.1"
                     /translation="MTLGLFDLPAPLLAWADEWITSVIPAPLSIALWAVLGGVVCLEL
                     YRVISPQERITRIKRDARSAQEELAAYDGEMEGAWPLMKSMLGLSLKRVGIVIPATLL
                     AAYPVIALLVWMSSAYGHVFPARG"
     gene            complement(377..706)
                     /locus_tag="VFY27_12590"
     CDS             complement(377..706)
                     /locus_tag="VFY27_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766401.1"
                     /translation="MNRSLNHVLVISSLACAGLLLPHTADAYVGPGAGLSLLGALWAL
                     IVAIAAAVAFIVAWPIRRMRRRKREERELAAAQERSEGAAEPSPENEMRRPPASAAQE
                     ATEPRRP"
     gene            complement(716..>2102)
                     /locus_tag="VFY27_12595"
     CDS             complement(716..>2102)
                     /locus_tag="VFY27_12595"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX5766402.1"
                     /translation="NLQTLRREGRMQKTVNAPARSRTSAADRFFFAAFIIAVAALLFI
                     GGSLATAVGITPGPQITRAYEGGKAFYAKLFHHDDVFSSDLWHPARTDLQGVSINDQR
                     AHNGVTLYTSGHEPAAYLISMEGELLHKWQRPFSQVWNESAAVKEPQPDSHVYMRKAI
                     PDTDGNLLVVYEGAGDTPYGYGMVKLDKDSNVIWSYLQPTHHDFDVAPDGRIFVLTHD
                     IIDEPSPELQHLRTPRLDDFLVILSPAGEELEKIPLLDAVEQSDYRQLLWEISSFALA
                     DPLHTNSVHVITEDEARRFTFGSPGQLLVSFREPSAIGVVDLDEQKLVWAAKGYWTGQ
                     HDPHILENGNILLFDNRGNFRKPEGRSRVIEFNPQTMQLVWQYTGTAESPLDSDIRSY
                     SQRLRNGNTLITESNGGRILEVTTAGDIVWEFTNPVRGGPENDRIPIICKALRLDAYL
                     AAAFLQVSLET"
BASE COUNT          375 a          647 c          670 g          410 t
ORIGIN      
        1 tcgccgcgcg ccgggaagac gtggccatag gcgctgctca tccacacgag cagtgcgatc
       61 accgggtagg cagctagaag cgtcgcgggt atcacgatgc cgacccgctt cagggaaagc
      121 ccgagcatcg acttcatcag cggccacgct ccttccatct ctccgtcgta cgccgcaagt
      181 tcctcctgcg cgctccgggc atcacgcttg atgcgcgtga ttcgctcctg cggagaaatg
      241 acacggtaca actcaaggca gacgacgccg ccgagcaccg cccacagtgc aatggaaagg
      301 ggggccggga tgacgctggt gatccattcg tcagcccacg cgagcagcgg cgcgggcagg
      361 tcgaacaggc cgagcgtcat ggacggcgcg gttcggtggc ttcctgtgct gccgaagccg
      421 gggggcggcg catttcgttc tcaggggacg gctcggcggc gccttcgctc cgctcctgcg
      481 ctgcggcaag ttcccgctcc tcgcgcttgc ggcgtcgcat gcgccttatc ggccacgcaa
      541 cgataaatgc gaccgcggcg gcgatcgcta cgatcagggc ccatagcgcg cccagcaggc
      601 tcaggccagc gcccgggccg acataggcat cagcagtatg cggtagcagc aaccccgcgc
      661 acgcaagact gctgatgaca aggacgtggt taagtgatct gttcatgagt caccgtcagg
      721 tttcgagtga gacttgcagg aaagcagccg ccaggtaagc gtcgagcctc agtgccttgc
      781 agatgatcgg aatccggtcg ttttcgggac cgccgcggac ggggttcgtg aactcccata
      841 cgatgtcccc cgccgtggtg acctccagga tccggccgcc attcgactcc gtgatcaggg
      901 tattgccatt gcgcagccgc tggctgtagg agcggatgtc gctgtccagc ggcgactccg
      961 ccgttcccgt gtattgccac acgagctgca tcgtctgcgg gttgaattct atgactcgcg
     1021 agcgtccctc cggtttgcgg aagttgccgc ggttgtcgaa tagcaggatg ttgccgtttt
     1081 ccaggatgtg cggatcgtgc tggccggtcc agtagccttt cgcggcccac acgagtttct
     1141 gctcgtcgag gtcgacgacg ccgatcgcac tgggttcgcg aaacgagacg agcaactgcc
     1201 ccggactccc gaaggtgaaa cgtcgcgctt cgtcttcggt aatgacatgc acgctgttgg
     1261 tgtgcagggg atcggcgagc gcgaaggaag aaatttccca caacagctgc ctgtagtccg
     1321 actgctcgac cgcatccagc aacgggattt tctccagttc ttctccggcc ggcgatagta
     1381 ttaccaggaa gtcgtcgagg cgcggcgtgc gaaggtgctg gagctccggc gaaggctcgt
     1441 cgatgatatc gtgagtcagg acgaatatcc tgccatcggg agccacgtcg aagtcgtggt
     1501 gggtgggctg caggtagctc catatgacgt tggagtcctt gtccagcttc accattccgt
     1561 agccgtacgg cgtgtctccc gcgccttcgt aaaccaccag caggttgccg tccgtgtcgg
     1621 gaatggcttt ccgcatatag acatggctgt ccggctgcgg ctccttcact gctgcgcttt
     1681 cgttccagac ctggctgaac gggcgctgcc acttgtgcaa caactcgcct tccatcgaga
     1741 tcaggtaggc ggccggttcg tgcccggacg tgtagagcgt cacgccgtta tgggcacgct
     1801 gatcgttgat ggacacaccc tgcaggtccg tccgcgccgg atgccagagg tcggaggaga
     1861 acacgtcgtc gtgatggaag agcttagcgt aaaaggcctt gccgccctcg taagcgcgcg
     1921 tgatctgcgg cccgggcgtg ataccgaccg ccgttgccag cgagccgcct atgaacagca
     1981 gcgctgcaac ggcaatgatg aaggcggcga aaaagaacct gtctgcggcg gacgtcctcg
     2041 accgcgcagg tgcgttgacg gtcttctgca tgcgcccttc tctcctcaag gtttgcaaat
     2101 tt
//
LOCUS       DASUOM010000605         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_809061,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000605 DASUOM010000000
VERSION     DASUOM010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809061"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFY27_12600"
     CDS             <1..483
                     /locus_tag="VFY27_12600"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX5766403.1"
                     /translation="YRFENRPDQRTKHSVFLQGKYFMDGKVLDMSWRYMTDDWGIDSH
                     TLDATFRWPLGERSWLEPHLRLYTQTEADFYRASLPAGELPAFASADYRLAAFDAFTA
                     GIKYGRRTSSGNELAVRLEYYRQDGEIPADQLFGDHSSEALYPGLDAIILNLSYGFDL
                     "
     gene            566..1396
                     /locus_tag="VFY27_12605"
     CDS             566..1396
                     /locus_tag="VFY27_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5766404.1"
                     /translation="MASPCQMLSRADRSTAREQVRTAAGEARRVEAKFSRYRVGNLID
                     RINKANGTPVEVDEETADLLDFAADLHRLSGGRFDISSGVLREVWTFDGSGRVPDAAQ
                     VERVLQRVGWDKITWERPLLTLAPGMQIDLGGIGKEYAVDRAARIAGSFSPAPCLINF
                     GGDVAAVAGTPGFDPWRVGIESIGAPSAKACRQIRLTRGGLATSGDTRRFLMKDGVRY
                     GHLLDPRTGWPVALAPRSVTVAADTCTQAGMLSTFAMLMGSAAETFLEQQRVRYWCIR
                     "
     gene            1534..2799
                     /locus_tag="VFY27_12610"
     CDS             1534..2799
                     /locus_tag="VFY27_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766405.1"
                     /translation="MICISLLALLSVLYAQSAGAMPAFARTYDISCNTCHSAFPRLSP
                     MGEMFADEWNFRLPNWKEQSLNLGDDRLYLPEQLPLGLRAQGFVQARDGEAIDPVTGD
                     VVADSSVDFQSPYLIKLLASAPLSEQISFYFYTIFAEKGGNGEVVVEDAWFSYGDLFG
                     TGVGMQLGQFQISDVMFPREVRLTFQDFMLYRMAGITYERGVLFGRSFGPVDVDIGFV
                     NGNGINANAGINSPGFRRADRLFDNDTSKTVFARAGTDVGGISAGLFALSGEQRNAAG
                     PAGMSSGDRDTDKRILGLDFSGEIGGGFHWFAQALWNEWDGFLDPAVSYEWYGAFAGV
                     DWVRDDFWTHSLLYNYADANDLDNTDTVFEGIDMSSLTFSSSYYFMRNVKGVIEFNVD
                     LLSTEEPGQFFTGHLSHEHYFLVGFDAAF"
     gene            complement(2766..>3777)
                     /locus_tag="VFY27_12615"
     CDS             complement(2766..>3777)
                     /locus_tag="VFY27_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766406.1"
                     /translation="AWGHIKPAWYLWTNAMPWLWLPVTLLVPWLIPGWARDLKERNTV
                     TLLLGAWVLLVLLFFSMSAGKRSLYIFPALPALALLAGPALPQLVARAAPRRVLFALG
                     LSLPLLLVAAGVYAVADPGFLQEKFAVPEATRSLAFSLAAIGLSGTAAVLAFRPRRAA
                     FGYAAIMAIVWSGVSLLVYPRIDATRSGEAIMEAAARVLGSHESLGFAGWKEQFLLQW
                     NRPAVHFGYRRNDAEGEIRDAAGWLSASPEHRLLLPQRMIEPCFDRSHLLPLGTAHRQ
                     QWLLADQRDVLPECRAVVTGAVFYDPSWPYAGRSNPTAQASAKPDRKHQHQNAASKPT
                     RK"
BASE COUNT          696 a         1179 c         1198 g          704 t
ORIGIN      
        1 taccgcttcg agaaccggcc ggaccagcgc acgaagcaca gtgttttcct ccagggaaag
       61 tatttcatgg acggcaaggt gctggacatg tcatggcgct acatgacgga tgattggggc
      121 attgactcgc acacgctgga tgcgacattc cgttggccgc tcggcgagag gagctggctt
      181 gagccgcacc tgcgcctcta cacgcagacg gaggcagact tctaccgcgc aagtctgccc
      241 gctggggaac tgccggcatt tgcgtccgcg gattaccgct tggccgcatt cgacgctttt
      301 acagccggca tcaagtatgg ccggcgcacg tcatccggca acgaacttgc ggtacggctg
      361 gaatactacc ggcaggatgg cgagataccg gccgaccagt tgttcggcga tcacagcagc
      421 gaggctctct atccgggcct cgatgccatc atcctgaatc tcagctacgg attcgacctg
      481 tgagactccc ttggcggcgc gcccggaacc agatgaaccg cgtctgaccc gccgcgacgg
      541 ttactgggtc ggcgagttcc gcgcgatggc cagtccgtgc caaatgctct cgagagccga
      601 tcgctctacc gctcgggaac aggtgcgcac ggccgccggc gaggcccggc gggtcgaagc
      661 gaaattcagc cgctatcgcg tcggtaacct catcgaccgg atcaacaagg ctaacggaac
      721 tccagtggag gtcgacgagg agacagctga cttgctcgat ttcgcagccg atttgcaccg
      781 gttgagcggc ggacgcttcg acatctcctc cggcgtgctg cgcgaggtgt ggacctttga
      841 cggcagtggc cgggtgccgg atgcggcaca ggtcgaacgc gtgctgcagc gcgtcggttg
      901 ggacaagatc acctgggagc ggcctttgct gacgcttgcg ccgggtatgc agatcgacct
      961 tggcggtatc ggcaaggagt acgccgtcga tcgtgccgcc cggatcgcgg gaagcttttc
     1021 tccagcgccc tgcctcataa acttcggcgg cgatgtcgcc gccgtcgcag gaacgcccgg
     1081 cttcgacccg tggcgggttg gcatcgaatc gatcggtgcg ccatcggcca aagcgtgccg
     1141 gcaaatcagg ctaacgcgtg gcgggctcgc gaccagtggg gacacacggc gcttccttat
     1201 gaaagacggt gttcgctatg gccacctgct tgatccgcga accggctggc cggtcgccct
     1261 cgcaccccgc tctgttacgg tcgcggctga cacctgcacg caggcgggca tgctgtctac
     1321 gttcgccatg ctcatgggct ccgcggccga aacgttcctg gagcagcagc gagtccgcta
     1381 ttggtgcatc cgctgatgag ccgaagcgtt gctcctgaaa tgcgccttca ccgctgttaa
     1441 gctaatgggg cgcctggcag ccagcaaacg cactcctccc cgtgagcttt ctaaaattcc
     1501 gttcgaggtc acgtcatggc accccgtccc tgcttgatct gcatcagctt gctcgccctt
     1561 ctctctgtcc tgtacgcaca gtccgccggc gccatgccgg ctttcgcccg aacctacgac
     1621 atctcctgca acacctgcca ctcggccttc ccgcggctga gcccgatggg cgagatgttc
     1681 gccgacgaat ggaacttccg cttgcccaac tggaaggagc agagcctgaa cctgggggac
     1741 gatcggcttt atttgcccga gcaattgccg ctcggcctgc gggcgcaggg attcgtccag
     1801 gcgcgcgacg gcgaagccat agacccggtc accggcgacg tggtggcgga ctcctccgtg
     1861 gatttccagt cgccttacct tatcaagctg cttgcgagcg ctcctttgag cgagcagatc
     1921 agcttctact tttacacgat cttcgccgag aagggaggca acggtgaggt ggtggtggaa
     1981 gacgcctggt tcagctacgg cgacctgttc ggtacgggag tcggcatgca actcggtcag
     2041 ttccagattt ccgacgttat gttccctcgc gaggtacgcc ttacgttcca ggatttcatg
     2101 ctctaccgca tggccggcat cacctacgag cgcggtgtgc tgtttggccg gtcattcggc
     2161 cccgtagatg tggatatagg ctttgtcaac ggcaacggca taaacgcgaa cgcaggtata
     2221 aacagcccgg gcttcaggcg tgccgaccgg ctgttcgaca acgatacctc gaaaaccgtg
     2281 ttcgccaggg ccgggaccga cgtcggtggt atttcggcgg ggctatttgc gctttccggc
     2341 gagcagcgga acgccgccgg accggccggc atgtccagcg gcgaccggga caccgacaaa
     2401 cgcatcctgg gcctcgactt ctcgggcgag atcggcggcg gtttccactg gttcgcccag
     2461 gcattgtgga acgagtggga cggttttctg gatccggcag tgagctacga atggtacggg
     2521 gcctttgcgg gggtggactg ggtgcgggac gacttctgga cacactcgct tctctacaac
     2581 tacgcggatg cgaacgacct cgataacact gacaccgtgt tcgaaggtat cgacatgagc
     2641 agcctcacgt tcagcagctc gtactacttc atgcggaacg tcaaaggcgt catcgagttc
     2701 aacgtggacc tcttgtccac ggaagaaccg gggcagtttt tcacgggcca cctcagccat
     2761 gagcactact tcctggtcgg cttcgacgct gcattctgat gctggtgctt tcggtcaggt
     2821 ttggctgacg cttgagcagt gggattgcta cggccagcgt acggccacga cggatcgtag
     2881 aagacggcgc ccgtcactac ggccctgcat tcgggcagca cgtctctttg gtcagcgagc
     2941 agccattgct gccggtgcgc cgttccgagc ggcaacaggt gcgagcggtc gaaacagggc
     3001 tcgatcatcc tttgcggcag caatagccga tgctccgggc ttgcggatag ccagcccgcc
     3061 gcatcccgga tttcgccctc ggcatcgttg cgacggtatc cgaagtgcac cgcgggccgg
     3121 ttccactgca gcaggaattg ttccttccag cctgcgaacc ccagcgactc gtgcgagccg
     3181 agcacgcgcg ccgcggcctc catgatggcc tcgcccgaac gcgtcgcgtc gatgcgcggg
     3241 tacacgagga gcgaaacgcc tgaccagacg atggccatga ttgctgcata cccgaacgcg
     3301 gctcttcggg gtcggaatgc gagtaccgca gccgtgccgc tcaaaccgat ggccgcgagg
     3361 ctgaaggcga gagaccgcgt ggcttccggc acggcgaatt tctcttgcag gaaccccggg
     3421 tcggcgaccg cgtatacgcc tgccgcaacc agcagtaacg gcaaagagag cccaagcgcg
     3481 aacaggacac gacgcggcgc ggctcgggcc accagctgcg gtagtgccgg tccggcaagg
     3541 agggcaagcg ccggtagcgc gggaaagatg taaagggagc gcttgcctgc gctcatcgaa
     3601 aagaacagca ggacgaggag cacccaggcg ccaaggagga gcgttaccgt attgcgctcc
     3661 ttcaggtccc tggcccagcc gggaataagc cagggcacca aaagcgtgac cgggagccac
     3721 agccacggca ttgcgttggt ccacaggtac cacgccggct tgatgtgtcc ccaggca
//
LOCUS       DASUOM010000606         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1773917,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000606 DASUOM010000000
VERSION     DASUOM010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773917"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFY27_12620"
     CDS             <1..895
                     /locus_tag="VFY27_12620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766407.1"
                     /translation="AAALFGVNLAFTQQYRYAAAESVEPGRAPRAISLVLLGAIGGAL
                     IGPELIRLGQHLIESVQFAGTLAALVVLYLVQSMLFLLLRPLRGEHEDKHAAPGRQLA
                     DIVRQPVFIVAVLGGTTAYGVMTLIMTATPLSMHINDGYSVEQTAHVIRTHVLAMYMP
                     SLFTGFLIERLGIGRLMTSGAVTLLVACIIGLQGQSVMHYTFALVLLGIGWNFLYVGG
                     TSMLTLSYSMSERFKAQAVNEFSVFGTSATASLLAGTVMYVYGWSTLIVLPLPLLILI
                     LGGLYIVRRDARVRRLSPASR"
     gene            907..2262
                     /locus_tag="VFY27_12625"
     CDS             907..2262
                     /locus_tag="VFY27_12625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5766408.1"
                     /translation="MHSAFIYHGDMYRFEVPQQSYWEATRAGTALAAAQLAANERCDV
                     AIIGGGYTGLSAAYHLARDHGIAARVLEAGHIGWGASGRNAGFCGIGGTSLSLKQMLR
                     KYGLEETRRFYRSQVNAVELVRQILSDEAIDADARGKVELEVAPSAKAFAGLAEHATR
                     QRELLGMDNRVLSPGEFRERYFDSTELHGAVVQRPAFSLHPMRYLLGLAAAAERHGAV
                     LHPRSEVLEWNKSGAEHILVTRGGTLRARSVLFATNGFMPEHLHKGFYGRPLPMISAI
                     VVTRPLTAEELAAQGWKTTEQAITSKRILNYYRLLPDNRLLFGGRGHATGSNAAATGT
                     FAALSRRIGEMWPHFKEIPIEYRWHGLICITMRLTPAIGRLADDSSVLFAYGYHGNGV
                     NTATWCGRKLAECVAGNHRLLDSLPAMVQGISPQFPFARLRLRYLQARLAMFRLQDAL
                     D"
     gene            2463..2654
                     /locus_tag="VFY27_12630"
     CDS             2463..2654
                     /locus_tag="VFY27_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766409.1"
                     /translation="MRELTKAELSFVSGGTGQCTPEGSGNSYSGVTDTSSFGDDVINL
                     YEGLVAATSHIIERVANAL"
     gene            complement(2651..3496)
                     /locus_tag="VFY27_12635"
     CDS             complement(2651..3496)
                     /locus_tag="VFY27_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5766410.1"
                     /translation="MEGNAYRPVFRVSVLAIMLFQVCALFARSALDISLVKRGMDRAI
                     ANDVSYLVVPPILAAFMYPYLRRHWTSLRSLFRPKALTLRLFMLAVILGLLLRVTYWA
                     LLTVLIWAGVIGNDDPDAVIGPLLGFDCPPLPVLALSVLTMSLMIPLVEEIINRGFLL
                     HALLPRGVVASVLLSAFLFALAHQPGTYVAAFAAGILLAVQTLNSQSLWPSLITHSTY
                     NLAAIVDWECFQIIWNPTMSDQTLITITKVAAPVAVGGIVLCCWLVCKKAIGTTVPRL
                     PRSHL"
     gene            3784..4212
                     /locus_tag="VFY27_12640"
     CDS             3784..4212
                     /locus_tag="VFY27_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5766411.1"
                     /translation="MTNDPNIVTAATLGAQVVLPTADYLKLPEYANADPAYGAQLATQ
                     CRACHSFEQGGPSVSGPNLFGVFGRQAGEVDGYPYSEALAEADFIWTPEALDAWLMRP
                     MRFLPGNRMAYPGLREQSDRNAVIAELLRVTDDSSAGDGQ"
     gene            4275..4745
                     /locus_tag="VFY27_12645"
     CDS             4275..4745
                     /locus_tag="VFY27_12645"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX5766412.1"
                     /translation="MGESREAETATESRDVADRRAFGWRTVIFGFLRSRRREHRRSAE
                     DEPVFIDWHHPWLFFLAVGTMLLSCLDAFFTLRLLDRGAIEVNPVMALAISHGTFAFT
                     VSKMLMTGLGILMLVFLSRSRLFNRVRTGVFLTVFFSVYACVVCYQFVLLLKQA"
     gene            4896..>5879
                     /gene="sucA"
                     /locus_tag="VFY27_12650"
     CDS             4896..>5879
                     /gene="sucA"
                     /locus_tag="VFY27_12650"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5766413.1"
                     /translation="MSNSLKEQYASSPLFGLNAAAVEALYERFLENPGSVQASWRAYF
                     DSLDGRKNDVPHSPVVRQIAEVTAISARNRTNGEAGAIRTSSLGAASEKQASVSRLIQ
                     VYSLRGHQIADLDPLGLMERPVPGVLKLDYLGLSEADMDTEFVTGGLAGTGNRRMKLR
                     DILALLQKIYCGKIGAEFAHMSRSRERLWLRKHFEQGIVAPGLSSEERLWILEQLTSA
                     EGIERYLHTRFVGQKRFSLEGGESLIPMLDDLIQRGGEAGIHEFVIGMAHRGRINVLV
                     NVLGKPPEELFEEFEGNVNPDEVKGSGDVKYHKGFSADMKTPGGNVHIALAF"
BASE COUNT         1178 a         1721 c         1810 g         1170 t
ORIGIN      
        1 ggctgcagcc ctcttcgggg taaacctcgc tttcacccag caataccggt acgcggcagc
       61 cgaaagcgtg gagccaggtc gcgcgccgcg cgcgatttca ctggtcctgc tcggggcaat
      121 cggcggggcc ctgatagggc cggagctgat aaggctcggg cagcacttga tcgagagcgt
      181 ccagtttgcc ggcacgctgg ccgcgctggt cgtgctctac ctcgtccagt caatgctgtt
      241 cctgctcttg cggccgctgc gcggcgagca tgaggacaag cacgcggccc cgggacgcca
      301 gctcgcggat atcgtgcgcc agccggtgtt catcgtggcg gtattgggcg gaaccaccgc
      361 ctacggcgtc atgacgctga tcatgaccgc gacgccgctc agcatgcaca tcaacgatgg
      421 atactccgtc gaacagacgg cgcatgtgat tcgcacccac gtgttggcga tgtacatgcc
      481 gtcactgttc accggcttcc tgatcgaacg gctcggcatc gggcgtttga tgacgtccgg
      541 tgcggtcacg ctgctggttg cctgcatcat cgggctgcag ggacagtcgg tcatgcacta
      601 tacgttcgcc ctggtattgc tcgggatcgg atggaatttt ctctacgttg gcggcacctc
      661 gatgctgacg ctgtcttact ccatgtccga acgattcaag gcccaggcgg taaacgagtt
      721 cagtgttttc ggtacttccg caaccgcttc gctgctcgcc ggtacggtga tgtacgtgta
      781 tggctggagc acattgatcg tactgccttt gccgttgctt atcctgatac ttggcggctt
      841 gtacatcgtg cgtcgcgacg caagggtgcg ccgcctgtct ccggcaagca ggtaacggga
      901 gcgccaatgc acagcgcgtt catctatcac ggcgacatgt accggttcga ggtaccgcag
      961 caatcgtatt gggaagccac ccgcgccggt accgccctgg cggcagcgca gcttgcggca
     1021 aacgaacgct gcgacgtggc gattatcggt ggcggttata ccggcctgag cgcggcctat
     1081 cacctcgcca gggaccatgg aattgctgca cgtgtactgg aggcaggaca catcggctgg
     1141 ggcgcctccg gacgaaatgc cggattttgc ggcatcggcg gaacttcgct gtcgctcaag
     1201 caaatgctca ggaaatacgg cctggaggag acacgccgtt tctatcgctc ccaggtgaac
     1261 gcagtggaac tcgtgcggca gattctttcc gacgaagcga tagacgcgga cgcacgcggt
     1321 aaagtggaac tcgaggttgc gccctcagcg aaagcgtttg caggactggc ggagcatgca
     1381 acccggcagc gggaactgct gggcatggat aatcgcgtac tttcgcccgg agagtttcgc
     1441 gagcgctatt tcgactcgac ggaactgcat ggcgccgtgg tgcagcgccc ggcattctcg
     1501 ctgcatccaa tgcgctactt gcttggactc gccgccgccg ccgagcggca cggagccgtc
     1561 ctgcacccgc gcagcgaggt actggaatgg aacaagagcg gtgcggaaca catcctggta
     1621 acccgtggtg gaacactgcg ggcgcgcagt gtccttttcg ccaccaacgg cttcatgccc
     1681 gagcacttgc acaagggatt ttacgggcgt ccgctgccca tgatcagtgc gatcgtcgta
     1741 acccggccct tgacggcaga agagctggct gcgcagggct ggaaaacgac cgaacaggcg
     1801 atcacgtcga aaaggatcct gaattactac cggttgctgc cggacaatcg tttgctcttt
     1861 ggcggcaggg ggcacgccac ggggagcaat gcggctgcaa ctggaacgtt tgccgcgctt
     1921 tcccggcgga tcggggagat gtggccgcac ttcaaggaaa tcccgatcga gtatcgctgg
     1981 cacggcctga tttgcatcac aatgcggctg accccggcca taggacgcct ggctgacgac
     2041 agttccgtgc tgttcgcgta cggctaccac gggaacggag tcaacactgc cacctggtgc
     2101 ggcaggaagc ttgcagagtg cgttgctggc aaccatcgac tactggattc gctgccggcg
     2161 atggtgcaag ggatctcgcc gcaattcccg ttcgcgcgac tccggctgcg ctacctgcag
     2221 gcgcggttgg cgatgttccg cttacaggat gcgctggact gacacgctat gaggttcgtc
     2281 gcttcattca gcagaaccgg aagctgaagc tgatcggccc cccggttgcg acgggtatcc
     2341 gctccgtttc ctgctccttt accgatttat gcgtgtcgct gcgagcgggt ggaaaaacta
     2401 cggtcacctt gcgcaggatg cgcaaaacca aatgacgaca agtcaaaatg gaggttgatg
     2461 tcatgcgaga acttaccaaa gcagaactct ctttcgtttc cggtggcacc ggacaatgca
     2521 ctcctgaggg atcgggtaac agctattccg gtgtgaccga tacatcttcg ttcggtgacg
     2581 acgtgatcaa tttgtacgag ggattggtcg ctgcaacgtc acacatcatc gaacgagtcg
     2641 cgaacgctct ctaaaggtgc gaacgaggca atcggggcac ggttgtcccg attgcctttt
     2701 tgcacacaag ccaacagcaa aggacgatgc ctcccacggc aaccggagcg gcaactttgg
     2761 tgatagtgat caacgtttga tccgacattg tcggattcca gataatctgg aagcattccc
     2821 aatctacgat tgcggccagg ttgtacgtgc tgtgggttat gagcgatggc cagaggctct
     2881 gcgagttcag agtctgcaca gccagcaaaa tccccgcagc gaaagcggcg acgtacgtgc
     2941 caggctgatg cgccagtgcg aaaaggaaag cagaaagaag aactgacgcc acgacccctc
     3001 tcggcaggag tgcgtgcagc aggaaacccc tgttgatgat ctcttctaca agggggatca
     3061 tcagtgacat cgtgagcacg ctgagggcca ggaccggcaa cggagggcaa tcaaagccca
     3121 ggagtggccc gattacagcg tcaggatcgt cgttgcctat gacgccggcc cagatgagca
     3181 ccgtcaggag tgcccaatac gtgacgcgca gcagcagacc caggatgaca gcaagcatga
     3241 acaaccgtag ggtcaatgcc tttggtcgga acaggctgcg cagcgaagtc cagtgccggc
     3301 gaaggtaagg gtacatgaat gccgccagaa tcggcggcac cacgagatag ctgacgtcgt
     3361 ttgctatcgc acggtccatg cctctcttga cgagtgaaat gtccaacgcg ctgcgtgcga
     3421 agagcgcaca cacctggaac agcatgatag ccagaactga tacgcggaaa accggcctgt
     3481 acgcgttccc ttccatgtgc aagttccaag gcgtcggatc aagcgggata gcgtattaac
     3541 gcctggttga tatggccgca gttctttctc gtgttgttgc ccggaaacga gtcacacggc
     3601 ttatgtcatg tctgttcact tttcgtttga taaatcctgg tctgtcacag aaaatgcggt
     3661 gcttcgccga cggcgcctgc acatcacctc attatgctga acctacgtgt tccggtcctg
     3721 ctggccgcca gtgctttgct ggcagcatgt ggcgggccgc aatcaggcga gcagaataca
     3781 acgatgacca acgacccgaa tatcgtgacc gcagcgacgc ttggcgcgca ggtcgtgctg
     3841 ccgacagcgg attacctgaa gctgccggag tatgcgaatg ccgatcctgc ttacggtgcg
     3901 caactggcaa cgcagtgccg cgcgtgccac agcttcgagc aaggaggccc gtcggtgagc
     3961 ggccccaatc tgttcggcgt gttcggccgg caggcaggtg aggtggacgg atatccctac
     4021 tccgaagcac tcgccgaagc cgattttatc tggacgccgg aggctctcga tgcgtggctc
     4081 atgcggccga tgcggttcct gccaggcaat cgaatggctt atcccggcct gcgcgagcag
     4141 agcgaccgca acgccgttat tgccgaactc cttcgcgtga cggacgattc gtcggcagga
     4201 gacgggcagt agccgcggaa gggccggccg aacggccaac tgccattttc aacatcatca
     4261 gggggagata ggctgtgggc gagtccaggg aagcggaaac ggcaaccgaa agccgcgacg
     4321 tcgccgaccg gcgcgcattc ggatggcgca ccgtcatctt cgggttcctg cgctcacggc
     4381 ggcgcgaaca ccggcgttcg gcggaagacg agccggtatt catcgattgg catcatccct
     4441 ggctgttctt cctggccgtc ggaacgatgc tcctgagctg cctcgatgcg ttcttcacgc
     4501 tgcgcttgct ggatcgtggt gcgattgagg tgaaccctgt aatggccctc gcgatcagtc
     4561 acggcacgtt cgcgttcacc gtcagcaaga tgctcatgac gggactcggc atcctgatgc
     4621 tggtgttcct ctcccgctcc aggctgttca atcgtgtgcg gacaggtgtt ttcctcaccg
     4681 tcttcttcag cgtctatgcc tgcgtggtgt gctaccagtt cgtgcttttg ctgaagcagg
     4741 cctgatgggc gtccgattcc ctaagcggag gccatacgat aaactagccc agattttctt
     4801 cacagcgtcg gctgcttaag ccttgcgctg ctgtttagtg gccttacctc tcatatatat
     4861 cgcgtaacct ccggttaggc gagccaactt ctgctatgag caattcgctg aaagagcaat
     4921 atgcttcgtc gccactgttc ggcctgaacg ccgccgcggt cgaggcgttg tatgagcggt
     4981 ttctcgagaa cccgggctcg gtacaggcca gctggcgcgc ctacttcgat tctctcgacg
     5041 ggcggaagaa cgacgtgccc cactcgccgg tcgtgcggca gatagcggaa gtaaccgcca
     5101 tttccgcgcg caatcgcacc aatggcgagg ccggcgcgat ccgtacctcc agtctcggcg
     5161 cggcgagcga aaagcaagcg tcggtatcgc gcctgatcca ggtttacagc cttcgcggcc
     5221 accagatcgc cgacctggat cccttgggac tgatggaaag gcccgtaccg ggagtgctca
     5281 agctcgatta cctcggtttg tccgaagccg acatggacac cgaattcgta acgggcggac
     5341 tcgcgggcac cggcaaccgg cgcatgaagc tgcgagatat cctcgcactg ttgcagaaaa
     5401 tttactgcgg caagatcggc gccgagttcg cgcatatgtc ccgctcccgc gagcggctgt
     5461 ggctcaggaa acatttcgag caggggatcg tcgctcccgg tctttccagc gaagaacggc
     5521 tgtggatcct cgagcaactg acatcggccg agggaataga acgctacctg catacgcgct
     5581 tcgtcggcca gaaacggttt tcgctcgagg gcggcgaaag cctgatcccg atgctggacg
     5641 atctcatcca gcgaggcggc gaagccggca tacacgagtt cgtaatcggt atggcgcacc
     5701 gcgggcgaat caacgtcctt gtgaacgtac tgggaaaacc gccggaggaa ctcttcgagg
     5761 aattcgaggg caatgtcaac ccggacgagg tgaaggggtc cggcgacgtc aagtaccaca
     5821 agggcttctc cgcggatatg aaaactcccg gcggcaacgt gcacatcgcg ctggcgttc
//
LOCUS       DASUOM010000607         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_329047,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000607 DASUOM010000000
VERSION     DASUOM010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329047"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..>2042
                     /locus_tag="VFY27_12655"
     CDS             248..>2042
                     /locus_tag="VFY27_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766414.1"
                     /translation="MKVAIQVILLSGSVALAATDVLAQAQALEEIVVTARKRAETLEE
                     APVSVRAFTEAEIASAGIETPHDFIGLTPNVTLVQTQNAGNSFINVRGISQARNSEMS
                     VAVLVDGVLMSNPAQFNQQLFDIQQIEVLRGPQGALYGRNAIGGAITIVTQQPTDETA
                     GKFEIGADSGPGYTARGTISGPLGNSDTLKYRLSGSYVDTDGYIDNTFLGEEADPYKD
                     VSGRLKLLWEPSNAFSADLRLSASALDTQALYFNIGAEVDDTSLPVRVNNPGVNERDM
                     LSFSLKLEWDGDFGSFTSITAYDDLEELLTGDQFSFLPREESFLNVETSGFGDFIRFL
                     TGQAVVDLSQTQFLDTDSLSQEFRFASPTEQPVRWIVGAYGISTDRSISTGNQIDLGN
                     GVFPVYRDPRPSVFTDGMTDPSPQLGLLEDGQDNFAWAVFGEVAWDLSDAFEAAVSLR
                     YDEDTRENTTLTEPPHGNFTGIEIVPGTVREETWSDLQPKLTLRYTPNDDVTVYGGYS
                     RGFRSGGFNQSGVGLAVPEPGIEDLFDEQIADTYEVGVKSVFADGRIFTSLSAFYTDF
                     EGAYFFFFDPGTSTQNLGSIPETEYMGFEFEG"
BASE COUNT          417 a          605 c          615 g          405 t
ORIGIN      
        1 ccgagcgcct gcagtatcat caattcagtg aaagagtagg tgcccatgat ctcatccacc
       61 aggttgcggc cccggaccat gatccggtcg gagtcgaaat ggcagatcgc cgtcgcggca
      121 cgagattttt tcctgtcagc catcaaaggg ctcctctcga ttttttcgtt agccgaattt
      181 gatttcgcta tgcgaaaatc atagcatgcg ggcgaatgct gttgcagaac atacggggga
      241 gcaacaaatg aaagtggcga tccaggttat cctgctttcg gggtcggttg cgctggcggc
      301 gactgacgtt ctggcgcagg cgcaggcact cgaggaaatc gtcgtaacgg ctcgcaagcg
      361 agctgaaacg ctggaggaag ccccggtttc cgtgagggct ttcaccgagg cagagatcgc
      421 gtcggccgga atcgagacgc cgcacgattt tatcgggctg acgccgaacg tcaccctggt
      481 gcagacgcag aatgcgggca actccttcat caatgttcgc ggtatttccc aggcacggaa
      541 cagcgagatg tccgtggcgg tgctggttga cggtgtgctg atgtccaacc cggcgcaatt
      601 caaccagcag ttgttcgaca tccagcagat cgaggttctg cggggacccc agggagccct
      661 gtacgggaga aacgcgatcg gcggggcaat taccatcgtc acgcagcaac ccaccgacga
      721 gacggccggc aagttcgaaa tcggcgccga ttccggcccc ggttacaccg cccgcggcac
      781 gatcagcggt cctttgggca attcggacac gttgaagtac cggctgtccg gttcctacgt
      841 ggataccgat ggctacatcg acaacacgtt cctcggcgag gaagccgatc cctacaagga
      901 cgtctcggga cggctgaagc tgctgtggga accgtctaac gcattctcgg ccgatttgcg
      961 cctcagcgcc tcggcgctgg acacgcaggc actctatttc aacatcgggg ccgaggtcga
     1021 cgatacgagc ctgcccgttc gcgtgaacaa ccccggcgtc aacgagcgcg acatgttgtc
     1081 gttcagcctc aagctggaat gggacggaga ctttgggtcc ttcacgtcca tcacggctta
     1141 cgacgatctc gaagagctgc tgaccggcga ccagttcagc ttcctgccgc gtgaggaatc
     1201 gtttctgaac gttgagacat ccggcttcgg ggacttcata cggttcctca ccggtcaagc
     1261 cgtggttgac ctgagccaga cacaatttct cgacaccgat tccctgagcc aggaattccg
     1321 ctttgcctct ccgaccgagc agcctgtgcg ctggatcgtc ggagcctacg gcatctcgac
     1381 cgaccgctct atctcgaccg gcaaccagat cgacctgggc aacggcgtgt tccctgttta
     1441 tcgcgacccg aggccgtcag tattcaccga cggcatgaca gacccgagtc cacagctcgg
     1501 cctgctcgag gatggacagg acaacttcgc ctgggcggtt ttcggcgagg tggcgtggga
     1561 cctgagtgac gccttcgaag cagccgtgtc gctgcgttac gacgaggata cccgcgagaa
     1621 cacgaccctt accgagccgc cgcacggcaa cttcaccggc atcgagatcg taccgggaac
     1681 ggtgcgtgag gagacctgga gcgatctgca acccaaactt acgctgcgct atacgccgaa
     1741 cgacgacgtc acggtgtatg gcggttacag ccgcggattc cgcagtggcg ggttcaacca
     1801 gtcgggcgta ggcctggcag tgccggagcc gggaatcgag gatcttttcg acgagcagat
     1861 cgccgacact tacgaagtgg gggtgaagtc ggtcttcgcc gatggccgga tctttacttc
     1921 gctgagtgcc ttctataccg acttcgaagg cgcgtatttc ttcttcttcg atcccggtac
     1981 cagcacccag aatctcggca gcattccgga aaccgagtac atgggattcg agttcgaggg
     2041 aa
//
LOCUS       DASUOM010000608         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_86723,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000608 DASUOM010000000
VERSION     DASUOM010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86723"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFY27_12660"
     CDS             <1..330
                     /locus_tag="VFY27_12660"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5766415.1"
                     /translation="SRHTRMSALQLRDLLRYAYRSRFMPEFISSMSLSGLDGTMSRRF
                     REDDVLAGMAHIKTGSLDDVTAIAGFLQARSGERYIVVSLHNDKNVHRGTGQEVQEAL
                     LRWVFER"
     gene            413..1015
                     /locus_tag="VFY27_12665"
     CDS             413..1015
                     /locus_tag="VFY27_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEX5766416.1"
                     /translation="MRLTVGPSLIALFLALPLPALAETAYVTDQLRLGLHRAEDTSDR
                     SFRTLQSGQELEILSQTRNYAQVRLPDGTAGYVKAAYLVTNKPAALIVSETQAENERL
                     QQELARTKAQFAGPAETIDALERQLAEQQAQLDEDSRRIAELQEQNESFVSRAQQFRF
                     SLPASWVAGATGLCLVAGFLCGLWWIDHRSRKRHGGFRIY"
     gene            complement(1039..1353)
                     /locus_tag="VFY27_12670"
     CDS             complement(1039..1353)
                     /locus_tag="VFY27_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5766417.1"
                     /translation="MKLQQLKYFLAIVDNGLNITAAADRLYTSQPGVSKQLKLLEEEL
                     GLKLFIRKGKSLDGLTAAGHQLIDRARLIMQEVENIRRVASSFSAEQDSQFRMMANQR
                     QA"
     gene            1615..>2306
                     /locus_tag="VFY27_12675"
     CDS             1615..>2306
                     /locus_tag="VFY27_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5766418.1"
                     /translation="MIYDSILGTIGRTPVIRLNRLAPAGIDMYVKVESFNPMASVKDR
                     LAIGIINDAEKKGLLSPGQTVVEATSGNTGVALAMVCAAKGHPFVATMAESFSIERRK
                     IMRGLGAKVILTPAAERGTGMVRRAEELAKKHGWFLARQFENPANPEYHRHTTGPEIL
                     QDFAGRRLDYWVTGYGTGGTMTGAGEVLKAGRPDITIVATEPAGASLLAGKEWNPHKI
                     QGWTPDFIPEVL"
BASE COUNT          461 a          689 c          702 g          454 t
ORIGIN      
        1 tcgagacaca cgcggatgtc ggctctgcag ctcagggact tgctccggta cgcgtaccga
       61 agccggttca tgccggaatt catatcttcc atgtcgctgt caggcctgga cggtacgatg
      121 tcgaggcggt tccgcgaaga tgatgtactc gccggcatgg cgcacataaa gaccggatcg
      181 ctcgatgacg tcaccgctat tgccggtttc ctgcaggccc gttccgggga gcgctatatc
      241 gttgtatcgc tgcacaacga caagaacgtc catcgcggca ccgggcagga agtacaggag
      301 gcgctgctgc gctgggtttt cgagagatag gcatcgcagc ctctataccc cgtcccggtt
      361 cggagacctg catcacataa gtcgtcgaca caccctgtag taagatcgaa cgatgcgtct
      421 tacggtcggg ccatcattga ttgcgctatt cctcgcgctg ccgttgccgg cgctcgcgga
      481 gacggcctat gttaccgacc agttgcggct cggcctgcat cgcgccgagg acaccagcga
      541 ccgttcgttt cgcaccctgc agagcggtca ggaactcgag atcctgtcgc aaaccaggaa
      601 ttacgcgcag gtaaggctgc cggacggtac ggcgggctac gtgaaagcgg cctatctcgt
      661 cacgaataag ccggcggcgc tgattgtgag cgagacgcag gcggagaacg aacgattgca
      721 gcaggaactg gcgcgcacca aggcgcagtt cgccggcccg gcggagacca tcgacgccct
      781 cgagcgacag ctcgcggagc agcaggccca gctggatgaa gacagtcgcc ggatcgcgga
      841 gcttcaggag cagaacgaga gcttcgtctc ccgggcgcag caattcaggt tcagcttgcc
      901 cgcttcctgg gtggctggcg ctaccggcct gtgcctggtc gcgggatttc tctgcggtct
      961 atggtggatc gatcaccgga gtcgcaaacg ccacggcggg tttcgcattt actagggagc
     1021 ctcgcgtcgg acgaggggtc aggcctggcg ctggttcgcc atcatcctga attgagaatc
     1081 ctgctccgcc gagaaactgg acgctacccg tcttatattc tcgacttcct gcatgatgag
     1141 ccgggcccgg tcgatcaact ggtgaccggc cgcggtcaga ccgtccaggc ttttcccttt
     1201 ccgtataaag agcttcaacc cgagctcttc ttcgagtaac ttgagctgct tgcttacgcc
     1261 gggttgggac gtatagagac gatccgccgc cgcggtaatg ttcagcccgt tatcgacgat
     1321 tgcaagaaaa tatttgagtt gttgtaattt catccactcg tgccgcccag gttcccgaca
     1381 cagaatagac ccgcagtttc actgttcgtt tcactgtttt ttgcactcgg cgttataact
     1441 ttgtgttatg agcactccgc gggcgttaca tgacgtggcg atcgcgggtc ctatttctgg
     1501 ggtatgattt tgagcattcc accgttcgaa caagtcctga ggccagagcg cgtcgtgctg
     1561 cagcggcagt gagagccatc gtggccgaaa aacaacgaag acgaggtata cctgatgatc
     1621 tacgacagca ttctcggcac aatcggccga acaccggtga tccgcctgaa ccgtcttgct
     1681 ccggccggca tcgacatgta cgtcaaggtg gaatccttca accctatggc gtctgtgaag
     1741 gacaggctcg ccatcggcat catcaacgat gccgaaaaaa agggtttgct gtcgccaggg
     1801 cagaccgttg tggaggcgac ctccggcaat acgggagtgg cgctcgcaat ggtgtgcgca
     1861 gcgaagggtc atcccttcgt cgcaaccatg gcggagtcct tctccatcga acggcgcaag
     1921 ataatgcgcg gtctcggcgc caaggtcatc cttaccccgg ccgccgaacg cggcaccggc
     1981 atggtgcggc gtgctgaaga gcttgcgaag aagcacggct ggtttctggc gcggcagttc
     2041 gaaaatccgg caaacccgga gtatcaccgc cacaccaccg gacccgagat cctgcaggac
     2101 tttgccgggc gcaggctcga ctactgggtc accggctacg gcaccggggg caccatgacc
     2161 ggcgcaggcg aagtcttgaa ggcgggccgc ccggatataa cgatcgtcgc gaccgaaccc
     2221 gcgggcgcct ccctgctcgc cggaaaggaa tggaacccgc acaagatcca gggctggacc
     2281 cccgatttca tcccggaggt gctgaa
//
LOCUS       DASUOM010000609         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_809540,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000609 DASUOM010000000
VERSION     DASUOM010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809540"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1420
                     /locus_tag="VFY27_12680"
     CDS             176..1420
                     /locus_tag="VFY27_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX5766419.1"
                     /translation="MISAVELFVGLRYLRAKRRTKFVSFITLISLLGIALGVAALIVI
                     LSVMNGFEAELRTRLLSMTAHGYVTGSDGRVQDWESLHAEIVATPGVQSAAPVIEMEG
                     MIRSGRVLTAVLVHGVLPEYERATSGNVVNYVEGSLDVLESDARHIVLGRSLAFDLGV
                     SIGDGLVLLMPRASGDGGMEPVLERFIVRGVFEAGVQEHDSSLALVHASDAGRLMALG
                     GAMTSVRFRARDVMEAPAISRSLQERLGEEYRSTDWTIENASYFRAIRLEKMMMSLIL
                     SLIIGVAAFNIVASLVMVVTDKTSDIAILRTLGMGPNDVVRVFFIQGAAIGWFGVFLG
                     VLSGILLALNVPELVPALEGMFGFQVMPGDVYYVSRIPSLLEVEDVTVIAIAAFALTS
                     LATLYPARRAAKVNPASALRYE"
     gene            1424..2683
                     /locus_tag="VFY27_12685"
     CDS             1424..2683
                     /locus_tag="VFY27_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX5766420.1"
                     /translation="MAVGYEPGYEFWVGTRYVRSRSSNRFVSLISAISMLGIAIAVAV
                     LIVVLSVVNGFERELRERLLAMSAHATIEDPRGRIEDWQVWVEEALDNPRVVAAAPYV
                     EGQGLLVHRDRLSGAELRGIQPKLENNVSGIAGVMAGGELASLEAGAFNIVLGVELAQ
                     ELQAAIGDKVTVTLAEGIVTPAGVLPRTKRFKVSGIYRVGMYEFDRRLALVNIEDASL
                     LYRLGGDATGVRLSVTDIYRAPEIVREVAMEAGGGVLVSDWTRRHVNFFRSIQITKSI
                     LFVILLMVVAVAAFNIVSTLVMVVKDKQSDIAILKTAGARPSSILKIFVTQGTIIGIA
                     GTAVGLALGVLLTLNLEAIVGFLEGTFGIKLLAADVYFISDLPAELDVGDVVRICTIT
                     LALALLSTLYPAWRAARTLPAEALRYE"
     gene            2676..3413
                     /locus_tag="VFY27_12690"
     CDS             2676..3413
                     /locus_tag="VFY27_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766421.1"
                     /translation="MNNRPETAAAGDTARQTASTPVLECRDVVRRFREGSEMLEVLSG
                     ADLAIGEAERVAIVGTSGSGKTTLLQIMGGLDDPTSGTVLVGGQSMANVGEKAKGELR
                     NRYIGFVYQFHHLLPEFTAEENVAMPLLIRHVRKPQALLQARALLSRVGLSERLSHKP
                     GELSGGERQRAAVARALITRPMLVLADEPTGNLDAGNGEHVLGLMLELNRELSTSLVI
                     VTHDHSIARRMDRILVLENGRLQPAAL"
BASE COUNT          624 a         1005 c         1183 g          729 t
ORIGIN      
        1 ttgacgaaaa tcgcacacca actccacccg ccgaggctcc gttcaccacc gccgtcacca
       61 gttcatgaat aacgcccacg ctgttccgca atcaaaggcc gtccgccctt gtccaacgtc
      121 cttacacccg ccagcaagcc gctgccgcac accggatgtg gtttaattgc ccgcgatgat
      181 aagcgccgtg gaactctttg tcggacttcg ctacctgcgg gccaagcggc gcaccaagtt
      241 cgtttccttc attacgctca tttcgctgct cggcatagca ctgggtgttg ccgccttgat
      301 cgttatcctg tcagtgatga acgggttcga ggcggagctg cggacgcggc tgctcagcat
      361 gacggcgcat ggatacgtga cgggcagtga cgggagggtg caggactggg agtcgctgca
      421 tgcagagatc gtggcgacac ctggcgtgca atcggcagcg ccggtgatcg agatggaggg
      481 aatgatacgc agcggccggg tgttgacggc cgtgctggtg cacggggtgc taccggaata
      541 cgagcgggcg acgtcgggta atgtggtgaa ttacgtcgag ggaagcctgg atgtgctgga
      601 gtcagacgcg cggcacatcg tgctggggcg cagcctggct ttcgacctgg gggtgtcgat
      661 cggggatggc ctggtgctgc tgatgccgcg tgcttcgggt gacggaggta tggaaccggt
      721 gctggagcgt ttcatagtgc gcggagtttt cgaagcgggc gtgcaggagc acgactcctc
      781 actcgcgctg gtgcatgcat ccgatgcagg aaggctgatg gcgctgggag gcgcgatgac
      841 gtcggtgcgc ttccgcgcca gggatgtcat ggaggcgcca gcgatctccc gttcgctgca
      901 ggagcgtctt ggcgaggagt atcgaagcac tgactggacg atcgaaaatg cgagctactt
      961 tcgcgccatt cgcctcgaga agatgatgat gtccctcatc ctgtccctga tcatcggcgt
     1021 agcggcattc aacatcgtcg ccagcctggt gatggttgtg accgacaaga ccagcgatat
     1081 cgcaatcctg cgcacgttgg gcatggggcc gaacgacgta gttcgcgtct tcttcatcca
     1141 gggggcggcg ataggctggt tcggcgtgtt tctgggcgtg ctgtccggca ttttgcttgc
     1201 gttgaacgtg cccgagctgg tgccggcgct ggaagggatg ttcgggttcc aggtgatgcc
     1261 cggcgacgtc tattacgtca gcaggattcc ttcgttactg gaagtagagg acgtgacggt
     1321 aatcgcaatc gccgcttttg ctctgacgtc gctcgcgacg ctttatccgg cgcggcgggc
     1381 ggcgaaggtc aatcccgctt ccgccttgcg gtacgaatga cgtatggccg ttggctatga
     1441 acctggttat gagttttggg taggcacccg ttacgtccgt tcgcgcagca gcaaccgctt
     1501 cgtttcgctg atctcggcga tctcgatgct cggcatcgcg atcgcagttg ctgtgctgat
     1561 cgtggtgctc tcggtggtga atggcttcga gcgtgaactg cgggaacggc tgctggcaat
     1621 gtcggcgcat gcgacgatcg aggatccccg gggccgaatc gaggactggc aggtgtgggt
     1681 cgaggaggct ctggacaatc cgcgggtggt tgcggccgcg ccttacgtcg aaggacaggg
     1741 attgctcgtg caccgggaca ggctgagtgg cgcggaactg cgaggtatcc agccgaagct
     1801 ggaaaacaac gtatccggca ttgccggcgt gatggcgggt ggcgagctgg cgagcctcga
     1861 agcaggagcg ttcaacatcg tccttggcgt ggagctcgcc caggaactgc aggcagctat
     1921 cggcgacaag gtcaccgtga cgctcgcgga aggcatcgtg acgcctgccg gagtcttgcc
     1981 gcgcaccaag cgcttcaaag tcagcggaat ttaccgggtc ggcatgtacg agttcgaccg
     2041 ccgcctggct ctcgtcaata tcgaagacgc atcgctcctt tatcgactgg gcggtgatgc
     2101 cacgggtgtg cggctttcgg tgacggatat ctaccgtgcg ccggagatcg tgcgggaggt
     2161 agcgatggag gcgggcggcg gcgtgctggt cagcgactgg acgcgccggc atgtcaattt
     2221 tttccgctcg atccagatca ccaaatcgat cctgttcgtg atactgctga tggtggttgc
     2281 agtcgcggct ttcaacatcg tctctactct ggtcatggta gtgaaggaca agcaatccga
     2341 catcgcgatc ctgaaaaccg ccggggcacg gccgtcgagc atcctgaaaa tcttcgtgac
     2401 gcagggcacg atcatcggca ttgccggtac cgctgtcggg ctcgctctcg gtgttttgct
     2461 gacgctgaat ctggaagcta ttgtcggttt tttggaaggg accttcggca tcaagctgct
     2521 ggcggcggac gtctatttca tcagtgacct gcctgcggag ctggatgtgg gggacgtcgt
     2581 caggatttgc acgatcaccc tggcgcttgc gctgctttcc acgctttatc cggcatggcg
     2641 cgccgcgcgc acgttgccag ccgaggcttt aaggtatgaa taacagaccg gaaacggcgg
     2701 cagccggcga taccgcaagg caaacagcga gtacgccggt tctcgagtgt cgcgacgtcg
     2761 tcaggcgttt ccgcgaaggc agcgagatgc tggaggtgct ttccggggcc gacctcgcga
     2821 tcggggaagc ggaacgtgta gccatcgtag gcacctccgg gtcgggcaag acgacgctgc
     2881 tgcagatcat gggcgggctc gacgacccca cttccggcac cgtgctggta ggcggccagt
     2941 cgatggcgaa cgtcggtgaa aaggcaaaag gtgaattgcg caaccgatac atcggcttcg
     3001 tctaccagtt ccaccacctg ttgcctgaat ttacggcgga ggaaaatgtc gccatgccgc
     3061 tcctcatccg gcacgtgcga aagccgcagg cgcttctgca ggcgcgcgcc ctgttatccc
     3121 gcgtcggcct ttctgagcgg ctgtcgcaca agcctggcga gctttcgggt ggagagcgtc
     3181 agcgcgccgc cgtggctcgc gcgttgatca cgcggccgat gcttgtgctc gcggacgaac
     3241 cgaccggcaa cctggatgcc ggcaatggcg agcacgttct cggcctgatg ctggaactga
     3301 accgcgaact cagcacgagc ctcgttatcg ttacacacga tcattcgatc gcccgtcgca
     3361 tggaccgcat actggttctc gaaaacggcc ggctgcagcc cgcagcactg taggagcccg
     3421 ccgcgcgggc gagtgcaatt tgcatcaggg aactcgccgc gtggcgggct cctggagttg
     3481 ccttagcact tatactgagc ctccggtggt gcacctggca gccgccgtta atccgtgtcc
     3541 c
//
LOCUS       DASUOM010000610         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_203803,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000610 DASUOM010000000
VERSION     DASUOM010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203803"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFY27_12695"
     CDS             <1..366
                     /locus_tag="VFY27_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5766422.1"
                     /translation="QAAIFSGLVGSVYSIEIIEPLANKAKETLERLGYDNVSTMLGDG
                     YYGWEEHAPFDAIVVTAAASHVPPPLIRQLKPGGRMVIPVGGRFLTQQLLLIEKQEND
                     EITTRQIAAVRFVPLTGER"
     gene            419..>2310
                     /locus_tag="VFY27_12700"
     CDS             419..>2310
                     /locus_tag="VFY27_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766423.1"
                     /translation="MTTRWVSPAVLLISTAAIAYEILLMRVLSIVHWHHFAYMIISVA
                     LLGYGASGTFIALARHALTRHFALAFSLSALAFALTMIGSYILGQRVPFNALEIVWNP
                     AEFLHLAAMYLAFFVPFFFAATCIGLAFTCGGDRISRIYFFDLLGAGIGALGIVLALF
                     VLWPQDVLRVLACVAVLAGLLPTLTPPLRYGLVAIYLLVIVAVLIALPHSLLELRMSP
                     YKGLKQTLEVMDARVLREQSSPLGLLTVVSSPTIPFRHAPGLSFGTRFEPPEQLAIFT
                     DGDAMTMMTRYTGNEAALGYMDDVTQALPYRLLQDPRVLVLGAGGGTDVLFALHHGAR
                     RVDAVELDPQLVQLVAKTYADFTGHLYEDERVRVHIAEARGFVRKSKNRFDLIQVSLM
                     DSFSASGAGVQALGESYLYTVEAIEDYLRHLEPGGFLAITRWIKLPPRDNLKLVATVR
                     AALVRSGNEEPGRSLAMIRSWNTATLLARNGEISAAEIERIRDFAARRSFDVAWYPNM
                     PRSEANRFNVLPAPWLYDGTVSLLSDNRDAFTDRYKFNLTPATDDRPYFFHFFKWSAF
                     EEMMSLREQGGAGLIEWGYLVLAGTLVQATIAGAVLILLPLSVIRPSWRRGTGRWMGG
                     YFLLLGL"
BASE COUNT          395 a          738 c          697 g          480 t
ORIGIN      
        1 caggccgcga tattttccgg gctggtcggg agcgtctaca gcatcgagat catcgagccc
       61 cttgcgaaca aggcgaaaga gacgctggaa cggctcggct acgataacgt ctctaccatg
      121 cttggcgacg gttactacgg ctgggaagag cacgcgccgt tcgacgccat cgtcgtgacc
      181 gccgccgcca gtcacgtgcc accgccgctg atcaggcagc tgaagcccgg cgggcgcatg
      241 gttatccccg tcggcggccg atttctgacg caacagctcc tgctgatcga aaagcaggaa
      301 aacgacgaaa tcaccaccag gcagattgcg gcggtgcgtt tcgtaccgct gaccggtgag
      361 cgatagcggc agcatcgcgc cacatgaacc ggccttttga ttccatggcg gcgcggtctt
      421 gaccacgcgc tgggtctccc ccgccgttct gctgatctcc acggctgcga tcgcttacga
      481 gatcctgctg atgcgagtgc tgtccatcgt gcactggcat cactttgcct acatgatcat
      541 cagcgtcgcg ctcctcggct atggtgccag tggaaccttc atcgcgcttg cgcgacatgc
      601 tctcacgcgc cacttcgcgc tcgccttcag cctcagcgcg ctcgcattcg ccctcaccat
      661 gatcggctca tacatcctgg gccagcgagt gccgttcaac gcgctggaga tcgtgtggaa
      721 tcccgcggag ttcctgcacc tggcggcgat gtacctcgct tttttcgtgc ccttcttctt
      781 tgccgcaacc tgcatcgggc tcgcgtttac ctgcggcggg gatcgcatca gccgcatcta
      841 cttcttcgat cttctgggtg ccggaatagg cgccctcggc atcgtgctcg cattgttcgt
      901 gttgtggccg caggatgtgc tgagggtgct ggcctgcgtg gcagtgctgg cgggactgct
      961 tcccacactc acgcctccgc tccgctacgg ccttgtagcc atttacctgc tcgtgattgt
     1021 ggcggttctg attgcgcttc cgcacagctt gctggaattg cgcatgtcgc cgtacaaggg
     1081 actgaagcag acgctcgagg tcatggacgc gcgtgtgctc cgggaacagt caagcccgct
     1141 cgggctcctg acggttgttt cgagtccgac gatccccttt cggcatgcgc cgggcttgag
     1201 ttttggcacg cgcttcgaac ctccggagca gctcgcaatc ttcaccgacg gcgacgccat
     1261 gaccatgatg acgcgctaca cgggtaacga agctgcgctc ggctacatgg acgacgtgac
     1321 gcaggccctg ccctaccgct tgctccagga tccgcgggtg ctcgtactcg gtgcaggagg
     1381 cggcactgac gtactgtttg ccttgcatca cggcgcccgg cgcgtcgatg ccgtggagct
     1441 cgacccgcaa ctggtacaac tagtcgcaaa aacgtacgcc gatttcaccg gacatctgta
     1501 cgaggacgag cgtgtccggg tccacatcgc agaagcccgc ggattcgttc gaaaaagcaa
     1561 aaaccggttc gacctgatcc aggtatctct gatggattcg ttttccgcgt ccggcgcggg
     1621 agtccaggcg ctgggagaaa gctacctcta taccgtggaa gccatcgagg attatttacg
     1681 acacctggaa ccgggtggct tcctcgccat cacccggtgg ataaagctgc cgccgcgcga
     1741 caacctcaag ctcgttgcga cggttcgggc cgcgctggtg cgcagcggca acgaggaacc
     1801 cgggcgaagc ctcgcgatga tccgcagctg gaacacggca acgctgctgg ccaggaatgg
     1861 cgagatttcc gctgccgaga tcgagcggat ccgtgatttt gccgcccgcc ggtccttcga
     1921 tgtcgcctgg tacccgaaca tgccgcgcag cgaagcgaac cgtttcaacg tgctgcctgc
     1981 accctggctc tacgatggca ccgtcagcct gctgagcgac aaccgcgatg ccttcaccga
     2041 tcgctacaag ttcaatctca ctcccgctac ggacgaccgg ccatacttct tccacttttt
     2101 caagtggagc gctttcgagg aaatgatgtc cctgcgggag cagggaggtg cgggccttat
     2161 cgagtggggc tacctggtgc tcgcaggaac gctggtccag gcaacgattg cgggcgcggt
     2221 cctgattctc ttgcccctgt ccgtgatccg gccctcatgg cgccgcggca cggggcgctg
     2281 gatgggaggc tatttcctgt tgctggggct
//
LOCUS       DASUOM010000611         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1774349,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000611 DASUOM010000000
VERSION     DASUOM010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774349"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4588)
                     /locus_tag="VFY27_12705"
     CDS             complement(<1..4588)
                     /locus_tag="VFY27_12705"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5766424.1"
                     /translation="MTEAPLPPAPLEHEAKALAVAHNASLSAGPRSFFARPDYRSLEE
                     DTNRVLERLHEEDPDRVRTYPYTEWLLDNGHILRATLQQIKKGLPGKYYRQLPTVAAA
                     SGGSRKTRVGALVDRAVEISQGPVEIDRLERFCQAYQATSPLTIGELWALPTMLRIRL
                     VRHVCSTAEDAWQLTRNDGRPEEIAELTSGIAGCVTSLRNMSAVQWESFVERLSVVDE
                     ILRQDPARMYALMDFDTRNDYRDVIERVSKASGWKERDVADAAVRLARQAGGEDSPYR
                     EQHVGYFLLDAGLRDLLRKLGAHGGMGRRILPARSTSGGVAYVAALSGLALGFCAFFA
                     SWLQEAAPLSLLILMSVIAAIPALTLSSDLVNTLILGIRRPHRLPKLDFSDGIPPQYR
                     AAVVVPAMLSSREAIDENVHMLELNWLGNSDTSMQFVLLTDHLDAPSERMPDDQVLLD
                     YVVAALERLNQRHGGKTGSPFVLLHRRRRWNEVASCWMGWERKRGKLVQFNDYLRGAR
                     GSDFEVQRGSVADAGNIRYVITLDADTLLPSGAAKKLIGAMSHPLNNAVLNPRTGRID
                     AGYSIIQPRIEINPTTSSSSAFARIYAGDVTLDLYSHAVSDIYQDLFGQGIFAGKGIY
                     DVDAFRATVHPQIPENRVLSHDLLEGLCGRAGLATDVVLLEDFPTSLLAHLKRMHRWV
                     RGDWQLLPWLFGCRPTPDSRRFRPGLVGGWQLFDNLRRSLLAPAILALLTLGWLFLPV
                     NLWLFTAVVMVAPGVGLLLHALSAFRTSTWRWGTARSSVLNVLDHAGRDLARWLLMLT
                     FLPVEAFVTLDAIVRTLYRLFVSRNKLLEWSTAAQVARDVTFATSASQFWRRMWPCPA
                     WAAVNAAALILVQPAALLPASLLLLLWAIAPLLAFRLSRPERREMPRIADEDQAWLRR
                     VARGTWAFFEQQVGPGTHWLPPDNLQIEPSPQTASQTSPTNIGFAMLSAAAAFDLGYT
                     GTREFVYNLHHTLQSILRLEKYRGHLFNWYSLIDIEPLAPRYVSTVDSGNFVAAMISV
                     RQSLLEMAHSPLRLTTVLHGLEDELGLIRETLAILRRRDNSRVLEELLAAVLDSEAEL
                     RSGHRPASVIRQLAEVHADALNSALLSTIDNEDLGWTSEEIRLLRTRVGNFTRRATAA
                     LSELRELFPWLLRTKRTDDLGTTALEEMSRELTLSHLAELPKCIENALERLDRAGHDS
                     ATEGASAFRQELLHASSGIRVLLDEVEALTANLGGLIRETDFSFLYNRERHLFHVGYR
                     VDTAELDSSYYDLLASEARLASFVAIAKGDVPPKHWVHLGRPLTSIKGLRVLLSWSAT
                     AFEYLMPRILMRTPRFGLLAQSCRGAVKQQRAFGQQHNIPWGVSESGYYHFDQHGRYQ
                     YQAFGIPLMGLKWDQGERLVISSYATFLALPFNAPATIENARRIEKLGGRGELGFYEA
                     LDFGTATGSHRAKPRIVQSYMAHHQGMTLAAIANLMTGDLLVERFHRDARIASAEYLL
                     YEQLPLRTQTQPLETFEAPLKQTEPSSVAIEYWSL"
     gene            complement(4592..5548)
                     /locus_tag="VFY27_12710"
     CDS             complement(4592..5548)
                     /locus_tag="VFY27_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766425.1"
                     /translation="MIRILVPLDGSPAAEMAIPHAVAIARAFSAEMELLGVVCEPGGM
                     FATPVNSLDWQLSKLQTEVYLSRTADTLDDRGLQVSWELREGDPAQAILQFARTSDID
                     ILVMTRYGTGDARQFRLGGTVQKVLSTSANSILLVDPAAHFDPAAGYAKVVAAVDGSQ
                     CSEWASAFAAMTAQAFEGSLQVLRVVEAPVLPGGTPLTAETRRFLEYIKRIAKSQASL
                     QLRNILSTIPPNVATTSSIVSSDDVVNTIESAAAASDADIVIMAAEDTQGDGSGYGSV
                     CDVLLARARFPLLVLRSEAAALSASHFRSVYLDDTEARADAV"
     gene            complement(5897..>7064)
                     /locus_tag="VFY27_12715"
     CDS             complement(5897..>7064)
                     /locus_tag="VFY27_12715"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5766426.1"
                     /translation="NSIPGHLDPTERERLRAAGTAAIEQVAIPAYEEFLVFFREEYKP
                     GARDDLGASSMPDGEAYYAELVKYFTTLDEATPDAIHRLGLEEVARIRAEMDGIIRQV
                     EFDGSFAEFIDYLRTDSRFYVDEAKDFLKEAAYIAKKIDGQMPAFFRTLPRLPYGIMP
                     VPDDIAPNYTTGRYWGAPIGGRRGGWYMVNTYALDTRPLFELPSLTAHEAVPGHHHQS
                     ALRQEIEGAPEFRRAFYPHAFGEGWGLYAEKLGIELGIYETPYDHFGRLSYEMWRACR
                     LVIDTGIHMKGWTREQAQQYLAENTALSLHNVRTEVDRYIAWPGQALAYKMGELKFLE
                     LRERAERELGPAFDIRDFHDVLLLSGGVTLNIVDDQVEQFIEEVKARSDAPGAQ"
BASE COUNT         1414 a         2099 c         2184 g         1367 t
ORIGIN      
        1 cgagcgacca gtattcgatg gccacggaag atggttctgt ctgtttgagc ggtgcctcga
       61 aggtctcgag tggctgcgtt tgcgtgcgaa gcggaagttg ctcgtacaac aggtactcgg
      121 cgctcgcgat gcgtgcgtca cggtgaaatc gttctacgag caggtcaccg gtcatgaggt
      181 tggcaatggc ggcaagtgtc atcccctggt ggtgtgccat gtatgactgg acgatcctgg
      241 gctttgcacg gtgactcccc gttgccgtgc cgaagtcgag tgcttcgtaa aatccgagtt
      301 ctccgcgacc cccgagcttc tctattcgtc tcgcgttttc aatggttgca ggggcattga
      361 atggcaaggc gaggaaggtt gcgtaactgg agatcaccag ccgctcgccc tgatcccatt
      421 ttagtcccat caggggtatg ccgaacgcct ggtactgata tcttccatgc tggtcaaaat
      481 ggtaataacc ggattccgaa actccccatg ggatgttgtg ctgttgtccg aacgcccgct
      541 gttgctttac tgcaccgcgg cagctctgcg cgagcagtcc gaagcgcgga gtccgcatca
      601 ggatccgtgg catgaggtac tcgaaagcag tcgcactcca cgaaagcagc acgcgcaggc
      661 ctttgatgct cgtcagcggc cgtccaaggt gaacccagtg cttcggtggc acgtccccct
      721 tcgcgatcgc gacgaaactg gcaagacgtg cctcggacgc gaggaggtcg tagtaactgg
      781 agtcgagctc ggcggtgtct acccgatacc ctacatgaaa gagatggcgc tcacggttgt
      841 agagaaagct gaaatccgtt tcccttataa ggccacccag gtttgcggtc agcgcctcca
      901 cctcgtccag cagtacgcga atgccgctgg atgcatgcag taattcttgc cgaaatgcag
      961 atgccccctc ggtagcgctg tcatggccag cccggtcaag gcgctcgagc gcattctcga
     1021 tacactttgg caattcagcc aggtgcgaca gtgtcagttc ccggctcatc tcttcgagtg
     1081 ccgtggtgcc caagtcatca gttcgctttg tgcgcagtaa ccacgggaac agctcccgga
     1141 gctcgctcag cgcagcggtt gcgcggcgcg tgaagttgcc gacacgcgtg cgaagcagcc
     1201 gtatctcttc actcgtccag ccaaggtcct cgttgtcaat ggtagacagt aacgcgctgt
     1261 tgagtgcgtc ggcatgaact tcggcgagct gccggatcac cgatgcaggt cggtgtccgc
     1321 tgcggagttc ggcttcgctg tcaagaacgg ctgcgagcag ttcttccagg acgcgggaat
     1381 tatcgcgccg gcgcaggatc gcaagtgttt cacgaatgag ccccagttcg tcttccagcc
     1441 cgtgaagaac agtcgtaagc cgcagcggcg agtgcgccat ctccaggagg ctctgccgaa
     1501 cggaaatcat tgcggcgacg aaattaccgc tgtccacggt ggacacgtag cggggtgcaa
     1561 gaggctcgat gtcaataagc gaataccagt taaacaggtg tccgcgatac ttctcgaggc
     1621 gcaggatgct ctgcagggtg tggtgcaggt tgtacacgaa ctcgcgcgta ccggtatagc
     1681 cgagatcgaa ggccgctgcc gcagagagca tggcgaagcc gatgttcgtc ggggaggtct
     1741 gtgaggctgt ctgtggcgat ggctctatct ggaggttatc gggcggcaac caatgcgtgc
     1801 cggggcctac ctgttgttcg aagaaggccc aggtaccgcg cgcgacgcga cgcagccacg
     1861 cctgatcctc atccgctatc cgcggcatct cacgccgttc gggcctgctc aagcgaaacg
     1921 caagcaacgg tgcaattgcc cacaggagca agaggaggga agccgggagc agagccgccg
     1981 gctgcacaag gatcagggcg gccgcattga ccgcagccca ggcggggcag ggccacatcc
     2041 gtcgccagaa ctgggacgcg ctggtagcga acgttacgtc gcgggcaacc tgtgctgcgg
     2101 tcgaccattc gagtagcttg ttgcggctga cgaacagccg gtagagggtg cggacgatcg
     2161 cgtccagggt cacgaaggct tcgacaggga ggaacgtcag catcagaagc cagcgcgcca
     2221 ggtcgcgccc ggcatgatcg agcacgttca ggacactcga tcgcgctgtg ccccagcgcc
     2281 atgtgctggt gcggaaagct gacagtgcat gcaggagcag ccccacgccg ggcgcgacca
     2341 ttacgacagc tgtgaacagc cacaggttga ccggcaggaa cagccagcca agggtcagta
     2401 acgccagaat cgcaggagca aggaggctgc gccgcaggtt atcaaacagc tgccagccgc
     2461 cgaccagccc cgggcggaat cgccgggagt ccggagttgg cctgcacccg aacagccacg
     2521 gcagcaactg ccagtcgccg cgtacccagc ggtgcatgcg tttcaggtgc gccagcaggc
     2581 tggtaggaaa atcctcgagc aggacgacgt ctgttgcgag accggcacgg ccgcagaggc
     2641 cttccagcag gtcatgacta agtacgcggt tttccgggat ctgcgggtga accgtggcgc
     2701 ggaaggcatc gacgtcgtaa atgcctttgc ctgcgaatat cccttgcccg aacaggtcct
     2761 ggtaaatgtc ggacaccgca tgcgagtaaa ggtcgagggt cacgtccccg gcataaatcc
     2821 tggcgaaagc gctggaggaa ctcgtcgtgg ggttaatttc gatgcgcggc tgaataatgc
     2881 tgtaaccggc atcaatcctg ccggtacgtg ggttcagtac agcgttgttc aacgggtgcg
     2941 acatcgcacc gatcagtttt ttcgctgcac cgctcggcaa taacgtatcc gcatcgagcg
     3001 tgatgacgta gcgaatgttc ccggcgtctg cgacggagcc gcgctgtact tcgaaatcgc
     3061 ttcctcgcgc acctcgcagg taatcattga actgcaccag ctttccacgc ttgcgctccc
     3121 atcccatcca gcaggatgca acctcgttcc agcggcgccg ccggtgaagc aatacgaacg
     3181 ggcttcccgt cttgccgcca tgtctctggt tcaacctttc gagcgcggct acgacataat
     3241 cgagaagaac ctggtcgtcc ggcatgcgct cggaaggcgc atcaagatgg tccgtcagaa
     3301 gcacgaactg catcgacgta tcactgttcc cgagccagtt cagctccagc atatgaacat
     3361 tctcatcgat cgcctcgcgg ctcgacagca ttgcgggaac cacgacagca gcacgatact
     3421 ggggtggtat accgtcggag aagtccagct ttggcagcct gtgcggccgc cggattccga
     3481 ggatgagggt gttgaccagg tccgacgaca gcgtcagtgc aggaatggcc gcaatgacgc
     3541 tcatcaggat gaggagggaa agcggcgccg cttcctgcag ccaggaggcg aagaacgcgc
     3601 agaatcccag cgccaggcct gacaatgctg cgacgtaggc gactccgccc gaggtacttc
     3661 gtgccggaag tatccttcga cccattcccc cgtgcgcacc aagtttgcga agcaagtcgc
     3721 ggaggcctgc atcgagcagg aagtatccga cgtgctgttc gcggtacggg ctgtcctcac
     3781 ctccagcttg cctggcaagc cttactgcag catcggcaac gtcacgttcc ttccatccgc
     3841 tggctttcga cacacgctcg atgacgtcgc gataatcgtt gcgcgtatcg aagtccatca
     3901 gcgcgtacat gcgggcagga tcctgtcgca ggatctcgtc aaccacgctg agacgctcga
     3961 caaatgactc ccactggact gcggacatgt tgcgcaggct ggtaacacat cctgctattc
     4021 cggaggtgag ttctgcgatc tcctccggtc ggccgtcatt cctggtcagc tgccacgcgt
     4081 cttcggccgt gctgcagacg tgccgcacaa gccggatccg caacattgta ggcaatgccc
     4141 agagttcacc tatcgtcagc ggggaggtcg cctggtacgc ctggcagaag cgctcgagcc
     4201 ggtcgatttc gaccggaccc tggcttatct cgacagcgcg atcgaccagt gcgccaacac
     4261 gggtctttcg agaacctccc gacgctgccg ctacggtggg gagctggcga tagtacttcc
     4321 ccggcagccc tttctttatc tgttgcagcg ttgcccgcag gatatgcccg ttgtccagaa
     4381 gccactcggt gtacggataa gtccgcaccc ggtcgggatc ttcttcgtga agacgctcca
     4441 ggacacggtt ggtatcttcc tcgagggaac ggtagtccgg ccgagcgaag aaagaccgtg
     4501 gcccggccga aagcgaagcg ttgtgcgcta cggcaagggc tttagcttcg tgctcgaggg
     4561 gcgcgggtgg aagaggcgcc tctgtcattc gctataccgc gtcggcgcgc gcttccgtgt
     4621 cgtcgaggta aacgctgcga aaatgacttg ccgacagcgc agctgcctcc gaacgcaaca
     4681 cgagtagcgg gaagcgcgct cgcgcgagca gcacgtcgca taccgagcca tagccactgc
     4741 catctccctg tgtatcctcg gcggccatga tcacgatgtc ggcgtcggat gcggcggcgg
     4801 cactctcgat ggtgttcacc acatcgtccg aggagacgat ggaactggtg gtcgccacgt
     4861 tgggcggaat ggtcgaaagg atattgcgca gctgcaaact tgcctgcgac ttcgcaatac
     4921 gcttgatgta ctcgaggaaa cgccgggtct cggctgtgag cggggttcct cccggcagaa
     4981 ccggcgcctc caccactcgc aggacctgta gtgacccctc gaatgcctgc gccgtcatcg
     5041 cggcgaatgc cgaggcccat tccgaacact gggagccgtc aaccgcggcg acgactttcg
     5101 catacccggc cgcagggtca aaatgtgcag cagggtcaac aagcaagatg gaattcgcgg
     5161 aggtggaaag tactttctga acggtgccgc ccaatcggaa ctggcgagca tctccggtgc
     5221 cgtagcgggt catcacaagt atgtcgatgt ctgaagtgcg ggcgaattgc aaaattgcct
     5281 gcgctgggtc gccctcccgc agctcccagc ttacctggag tccccggtcg tcaagtgtgt
     5341 cggcagtgcg tgacaggtaa acctccgtct gaagtttcga aagttgccaa tcaaggctgt
     5401 tgacgggtgt ggcaaacatc ccgccaggct cacagacgac tccgagcagt tccatctccg
     5461 cggaaaaagc acgggcgatg gcaacggcgt gcggtattgc catctcggca gctggggagc
     5521 cgtcaagtgg aacgagaatt cgaatcatgc ctgtctgtct ccgggcgcgt cgctgcgcct
     5581 gaacgaaaca aggagccggg ccggggccct gctcccgaag tgagctgatt agctccggag
     5641 tgtcttcaca tgtgggtacg gcagcgacct tcgcaaccgc atgtgtcata gcgcggataa
     5701 actaaagacg ttccggcggg ctttaatgtc agcgagcgtc actggagcaa accggcgcaa
     5761 aagcgcggcc cgcgctcggt cagcaacaga atgaccctga aaggagcccg aggaagggcg
     5821 aaaatgagga gctgttgcgt gaccgctcgc tacgtggaga ttttacgcgg gtcgccttga
     5881 acaaagcaag gacgcttcac tgcgcgccgg gagcatcgga tcgcgccttg acttcctcga
     5941 taaactgctc gacttggtcg tcgacgatgt tcaaggtgac gccgccactc agcagcagga
     6001 catcgtggaa gtcgcgtatg tcgaaagcgg ggccaagttc ccgttccgcc cgttcccgta
     6061 gttcgaggaa cttgagctcc cccatcttgt aagcgagcgc ctgtcccggc caggcgatgt
     6121 agcggtctac ttccgtccgc acgttgtgca gcgacagggc ggtgttctcc gccaggtatt
     6181 gctgtgcctg ctcgcgcgtc cagcctttca tgtgtatgcc cgtgtcgatc accaggcgac
     6241 aggctcgcca catttcgtag gaaagacggc cgaagtgatc gtatggcgtc tcgtagattc
     6301 ccaactcgat cccgagcttc tccgcgtaca gaccccaacc ctcgccgaag gcgtgaggat
     6361 agaatgcgcg tcgaaactca ggcgctcctt caatttcctg tcgcagcgcg ctctggtgat
     6421 gatgccccgg taccgcttca tgcgcagtca gcgacggtag ctcgaacagc ggccgcgtgt
     6481 ccagggcgta ggtgttgacc atgtaccagc cgccacggcg tccgccgatg ggagcacccc
     6541 agtagcgccc ggtcgtgtag ttcggtgcaa tgtcatcggg caccggcatg atgccgtagg
     6601 gcaatcgggg cagcgtgcga aagaaggccg gcatctggcc atcgatcttc ttcgcgatgt
     6661 aagcagcctc cttcaggaag tctttcgctt cgtcaacgta aaagcgcgaa tccgtgcgca
     6721 ggtagtcgat gaactcggca aagctgccgt cgaattccac ctgcctgata atgccatcca
     6781 tctcggcgcg tatacgcgca acttcctcga ggcccaggcg gtgaatggca tccggggtag
     6841 cttcgtcgag agtggtgaag tatttcacga gttctgcgta ataggcttcg ccgtccggca
     6901 tcgaggaagc acccaggtcg tcacgggcgc ccggcttgta ctcttcgcgg aagaaaacca
     6961 ggaactcttc gtatgcagga atcgcaacct gttcgattgc cgcggtgcct gccgcacgta
     7021 accgctcgcg ttccgtggga tcgaggtgtc ccggaatcga gttg
//
LOCUS       DASUOM010000612         6411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_212665,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000612 DASUOM010000000
VERSION     DASUOM010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212665"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VFY27_12720"
     CDS             complement(<1..1389)
                     /locus_tag="VFY27_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766427.1"
                     /translation="MCDAHSDVQEEQRRFDLLQQQLAAQFEQVFPDMQVPRTIVVVPS
                     LSLHPEELMKISGVHHYEERMLCLLMLLRMPRTRVVYVSSQPISPTIIDYYLHLLPGI
                     PTSHARQRLTTLSCHDGSHVPLTKKILERPRLLQRIRNELAGCQYGHMTCFNATPLER
                     TLALALNLPLYATDPALAHLGTKSGSREVFRAAGVAMPDGFENLRDDADLVAALAALK
                     QRQPTLRKAVVKLNDGFSGEGNALFSYEGCPSDTDCSVWVEQVMPQRLRFEAASETWD
                     HYRLEFAEMGGIVEVFLEGANKRSPSAQCRINPLGQAGSISTHDQVLGGPSGQVFLGC
                     TFPADEAYRLAIQEAGMRAAEILRQRGVLGRFGVDFISLPRAHGGWDHYGIEINLRKG
                     GTTHPFLMLQFLIDGRYDPASGCYYTPAGQPRYYYASDNLQHEAYKGLTPEDLIDIAV
                     YHDLHFHGATQQG"
     gene            1620..2099
                     /locus_tag="VFY27_12725"
     CDS             1620..2099
                     /locus_tag="VFY27_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5766428.1"
                     /translation="MNRRLMPFILALMLASAAHAQHAATAPAAEELTKLLTEFLDGAS
                     RNDVAAHDRFWADDLIYTRSSGVRTGKAEILENARSAPTTTAADSAEYSAEDIRIQQY
                     GDTAVVAFRLVATTGSGPERVVEYFLNTGTFVARDGEWRAVAWQSTRVPDPEAARRN"
     gene            2192..3481
                     /locus_tag="VFY27_12730"
     CDS             2192..3481
                     /locus_tag="VFY27_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5766429.1"
                     /translation="MVAVFDAALAVPVWLLSLLVLLVIGLGLVIWSLKHRRDPHFRIA
                     TDAPLPDLLPSVAGLTHGQLIDGNSVEVVENGEYFDLLLRDVQSAQRSVHFETFLWKD
                     GEASRLLSDALVERARAGVCVRVLVDARGGKEMGQETRHLLEQAGCTFAAFHPSGLRA
                     IGRINTRDHRKLAVIDGHTAWVGGHCIVDTWLGDAEDKEHFRDISVRLRGPIVHAIQS
                     TFSENWVMTTGELFLGDDVFPGLADEGDIPVHVARITPLGSASAVKILHHLVICCARK
                     RIRIQNPYFLPDPEGIELFAQAVRRGVDVRVMTPSTEASDMPIVQHAAHHNFGKLLSH
                     GVRVFEYQKTLLHQKVMTVDGCWSAIGSSNFDDRSFEINDEITLGFHDSALAAKLETI
                     FERDAEHCVELTRESWARRSLAHRFLDGALYLVNEQL"
     gene            3556..5427
                     /locus_tag="VFY27_12735"
     CDS             3556..5427
                     /locus_tag="VFY27_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5766430.1"
                     /translation="MTAAATPNWPAALTTAARLASFAIICGLLFWGQVILIPITLAAL
                     VTFLISPLVTRLDRLGLPRIVAVIVVSIGVTGLIGGIGYIVTSQLAELAQDLPEYREN
                     IRGKISDLRSMTRGGTIESVQDTIEGISADVKRDAAAEAPAATDDDDEKPMRVAIEPE
                     QQFIGESGFLSPVFEGAATAGLTMLLSIFMMIKREDLRNRLVSLAGQASLAVTTKAFS
                     EAGQRISRYLLMQFIINASMGLAVWLGLFLIDVPYSALWGLAAGVFRYIPYVGPWLAA
                     LLPITVSVITAPGWEQVVFVVGLFLTLELFSNNVMEPWLYGQSVGLSPVAVIVAAIFW
                     AWLWGPVGLVIATPITACLVVVSRYIPEMAAFDRLLSERPALQPHLWLYQRLLARDEE
                     EAGDIIEEHREEHSLSETCDQLLLGSLLALKRDLAAGRVNQEDGEFVESALREMVDEM
                     VESLDSKTADVDDADIDPAEQMPEPVLLIGMPVRDMLDDIALQLLRVLLREAHCTLEI
                     LSPETLIGERIAEVEERKPAAVCIASLPGDLAATRHVCKRLRARLPNLPLFVGRLLNS
                     RKAPERSVHLLKAAGAQHVAATLGEMRDLLQQLVRSKQTGLAHEQVQFDSGVAATLP"
     gene            complement(5435..>6411)
                     /locus_tag="VFY27_12740"
     CDS             complement(5435..>6411)
                     /locus_tag="VFY27_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5766431.1"
                     /translation="EPLSSTRVTFSRQERVTIPAGAYMVSDPVGMKVAALDHLAVSIY
                     FPSAPAQQTGHPGSRATSYIQRGNHVERENLPEARQVDHWYQLAGLDVEAGDTSRALL
                     VIGDSITDGFGVQPNTDRRWTDFLIARIREDPGLKGTLAVLNVGLGGNRLLLDGLGPN
                     GLARFDRDVLDRPGARQVIVALGVNDLGTLSRSESATQRDHENLVQQIIGAYEQMVAR
                     AREKDIEIIGATITPFRGSTFYPSDDLTEASRQKVNAWIRTPGNFDAVIDFDALLRSP
                     EDPARMDARFDSGDHLHPSVAGYEAMAKFVPLELFANSPPDPARARLR"
BASE COUNT         1202 a         1937 c         2023 g         1249 t
ORIGIN      
        1 gccctgctgc gtggctccgt ggaagtgcaa atcatggtac accgcgatgt cgatcaggtc
       61 ttcaggcgtc aggcccttgt aggcctcgtg ctgaaggttg tcggaggcgt agtagtagcg
      121 cggctggccg gcgggcgtgt agtagcagcc gctggctggg tcgtatctcc cgtcaatcag
      181 gaattgcagc atcagaaacg ggtgggtcgt gccgcccttg cgcaggttga tttctatgcc
      241 gtaatggtcc cagccgccgt gcgcccgtgg gagcgaaatg aagtcgacgc cgaagcgacc
      301 cagcacgccg cgctggcgga ggatctcggc cgcgcgcatt cccgcttcct ggatcgccag
      361 ccggtacgct tcatcggcgg ggaaggtgca gccgaggaat acctgccccg acggtccgcc
      421 aagcacctga tcatgggtcg agatggagcc tgcttgtccg agcggattga tgcggcactg
      481 ggctgagggt gaacgcttat tggctccctc taaaaacacc tcgacgatac cgcccatctc
      541 ggcgaactcg aggcgatagt gatcccacgt ctccgacgcc gcctcgaagc gcagccgctg
      601 gggcatgacc tgctctaccc acaccgaaca gtccgtatcg gagggacatc cctcgtacga
      661 aaagagcgcg ttgccttcgc cggaaaagcc gtcgttcagc ttcacgacgg ccttgcgcag
      721 ggtgggctgg cgttgcttga gggcggcgag agcggccacg aggtctgcgt catcgcgcag
      781 gttttcaaac ccatccggca tggcgacgcc tgcggcgcga aaaacttccc ggctaccgct
      841 cttcgtgccc aggtgcgcca gcgccggatc cgttgcgtag agcggaaggt tcagggcgag
      901 ggccagcgtg cgctccagtg gcgtggcgtt gaagcaggtc atatgcccgt actggcaacc
      961 cgccaactcg ttgcggatgc gttgcaacag acgcggacgc tcaagaatct tcttggtgag
     1021 gggcacgtgc gaaccgtcgt ggcaactcag cgtcgttagc cgctggcggg cgtggctcgt
     1081 ggggataccc ggcaacaaat gcaggtaata gtcgatgatt gtcggcgaaa tcggctggct
     1141 cgacacgtat acgacgcgcg tgcgcggcat ccgcaacagc atcagcaggc agagcattct
     1201 ctcttcgtaa tggtgcacgc ccgagatctt catgagctcc tccggatgaa ggctcaagct
     1261 gggaaccacc acgatcgtgc gtggcacctg catatcggga aagacctgct cgaactgggc
     1321 tgcaagctgc tgctgaagca ggtcgaagcg ccgttgctcc tcttgaacgt cagaatgagc
     1381 gtcgcacatt atcttcggtg aggagctggt ttgaggatgg ctggcctttt gcaagcatac
     1441 atgcccgcgg cgacaggcga cagcggtgcc tggctcggca aggtccatgt gttaatcacg
     1501 tgagccgatg gacgagccga ccttcgcttc gctgccaacg atcaacgccc gatgaagctg
     1561 aagtgcgaaa agaacgttat ccttcgagca tgtaccgata tttcgagtgg gggtgccgaa
     1621 tgaaccgacg actgatgccc ttcatcctcg cgctgatgct ggcgtcggcg gcgcacgcgc
     1681 agcacgccgc gacggcgccc gctgccgaag agctgacgaa actgctgacg gaattcctgg
     1741 acggtgcttc gcgtaacgac gtcgccgccc acgaccgctt ctgggcggat gacctgattt
     1801 atacacgctc ctccggcgta cggacgggca aggcggagat cctcgagaac gcacgctccg
     1861 ctccaacgac gactgcagcc gattccgccg agtattccgc cgaagatata cgcatacagc
     1921 agtacggtga caccgccgtg gtcgcctttc gcctcgtggc aacaacaggc agtggcccgg
     1981 agcgggtcgt cgagtacttc ctcaataccg ggactttcgt cgcgcgcgac ggggagtggc
     2041 gggcagtagc gtggcaatcc accagggtgc ccgacccgga ggcggctcgc cgcaattagc
     2101 caaagcctcc ggaggcaggt ccgcgtcggc caatcgctgt gcggcacagc ccgctcgagc
     2161 tagtgtgcct tgcgttgggt taggatgcgc aatggtcgcc gtattcgatg ccgcgctcgc
     2221 cgtacctgtc tggctgttga gcctcctcgt gctgctggta atcggactcg gcctcgtcat
     2281 ctggtcacta aagcatcggc gcgacccgca ctttcgcatc gcgaccgatg cgccgttgcc
     2341 ggatctcctg ccctcagtgg ctggcctgac ccacggccag ctcatcgacg gtaattccgt
     2401 cgaggtcgtg gagaacggcg aatatttcga tcttttgcta cgcgatgtcc agagcgcgca
     2461 gcgatcagtg cattttgaaa cgtttctctg gaaggacggc gaagccagca ggctattgtc
     2521 cgacgccctg gtggaacgcg cccgcgcggg agtgtgcgtg cgggtgctcg tcgacgcgag
     2581 aggcgggaag gagatgggcc aggaaacacg ccatctgctg gagcaagccg gctgcacgtt
     2641 cgctgccttc cacccgagtg gcttgcgcgc gatcggccgc atcaacaccc gggaccatcg
     2701 caagctggcc gtcatcgacg gccacaccgc atgggtcggc ggacactgca tcgtcgatac
     2761 ctggctcggc gatgcggaag acaaggagca cttccgcgat atctctgtgc ggctgcgagg
     2821 gccaatcgtg cacgcgatcc agtcgacgtt cagcgagaac tgggtgatga ccacgggcga
     2881 gctgttcctc ggcgacgacg tctttcccgg tctggcggac gagggcgata ttcccgtgca
     2941 cgtggcgcgc atcacgcccc tgggttccgc atcggcagtg aagatcctcc atcacctggt
     3001 tatttgctgt gctcgcaaac gaatccggat ccagaatcct tatttcctgc cggacccgga
     3061 agggatcgag cttttcgcac aggcggtaag acgtggagtc gacgtacggg ttatgactcc
     3121 atctaccgag gcatcggata tgccgattgt gcagcacgcc gcgcaccaca actttggcaa
     3181 gctgctgtca cacggtgtgc gcgttttcga gtaccagaaa acactgttgc accagaaggt
     3241 gatgacagtg gacggttgct ggtcggcgat cgggtcgagc aatttcgacg atcgttcctt
     3301 cgagatcaat gacgaaatca ccctcggctt ccacgattcc gccctggcag caaagctgga
     3361 aacgatcttc gagcgcgatg cagagcattg cgtggagctc acgagagaat cgtgggcgag
     3421 gcgcagcctg gctcatcgat ttctcgacgg cgccttgtac ctggtcaacg aacaactttg
     3481 atgtaggcgc attggaaagc gatccggtcg atcattatgg cgtagactgc tctttcaatc
     3541 gtggaacgac gagtgatgac tgccgctgca accccaaact ggcccgctgc gctgacgacc
     3601 gctgctcgac ttgcaagttt cgccatcatc tgcggcctgc ttttctgggg acaggtcatc
     3661 ctgatcccga tcactctcgc ggcgctcgtc accttcctga tcagcccact ggtcacgcgg
     3721 ctggacaggt tgggcctgcc acgcattgtc gcggtcatcg tcgtgtcgat cggtgtgaca
     3781 ggcttgatcg gcggcatcgg ttacatcgtc accagtcaat tggcggagct tgcgcaggac
     3841 cttcctgaat accgggagaa catccgcggc aagatctcgg atctccgctc gatgacgcgc
     3901 ggaggcacga tcgaaagcgt acaagacacg attgaaggca tcagcgccga cgtcaagcgc
     3961 gatgcggccg ccgaagcccc ggcagcgacc gatgatgacg acgagaagcc gatgcgcgtt
     4021 gcgatcgaac ccgagcagca gtttatcggt gaaagcggat tcctgagccc ggtattcgag
     4081 ggagccgcga ccgctggcct cacgatgctg ctttccatct tcatgatgat caagcgagag
     4141 gacctgcgta accgcctggt aagcctcgcc gggcaggctt cgcttgcggt gacgacaaaa
     4201 gcattctccg aggccggtca gcgcatcagc cgttacctgc tgatgcagtt catcatcaat
     4261 gcctcgatgg gcctggccgt gtggctggga ctgttcctga tcgacgtccc atacagtgcg
     4321 ctgtggggtc tcgcggccgg ggtattccgc tacataccgt atgtcgggcc gtggctggcg
     4381 gcgttgttgc cgattaccgt aagcgtcatt accgcgccgg gctgggagca ggtggtcttc
     4441 gtggtgggcc tgttccttac gttggagctt ttcagtaaca acgtaatgga gccatggctt
     4501 tatggacaga gcgtcgggct ctcgcccgtc gcggtcattg tcgcggccat cttctgggcc
     4561 tggctctggg gtccggtggg cctcgttatc gccacgccga tcaccgcttg cctggtggtg
     4621 gtatcgcgat acatcccgga gatggcggca ttcgaccggc tgctcagcga gcggccggcg
     4681 ctacagccgc acctgtggct ataccagcgc ctgctggcac gcgatgagga agaggccggg
     4741 gacatcatcg aggagcatcg tgaggagcat tcgctctccg aaacctgcga ccaattgctc
     4801 ctgggctcgc tgctcgcgct caagcgagac ctggctgcag gtcgcgtgaa tcaggaggat
     4861 ggcgagttcg tcgaaagcgc tttgcgggag atggtcgatg aaatggtcga gagccttgac
     4921 agcaaaacgg cagatgtcga cgacgcggat atcgatccgg cggaacaaat gccggagccc
     4981 gtactgctga tcggcatgcc cgtgcgcgac atgctcgatg acatcgcttt gcagcttctc
     5041 agggtcttgc tccgggaagc gcattgcacc ctcgagatcc tttctccgga gacactgatc
     5101 ggagagcgta tcgccgaagt cgaagaacgg aagccggccg cggtatgcat cgcatcgctg
     5161 ccgggtgacc tggcggcaac acggcacgtc tgcaagcgcc tgcgcgcacg cctgccgaac
     5221 ctccccctgt tcgtcggtcg cctgctgaac tcacgcaagg cgccggaacg cagcgttcac
     5281 ctcctgaagg ccgccggcgc gcaacacgtg gccgccacgc tgggagagat gcgggacctg
     5341 ctgcagcagc tggtgcgtag caagcagacg ggcttggccc atgaacaagt acagttcgac
     5401 tccggcgttg ccgccaccct gccatgagcc ggcatcatcg taaccttgcg cgagcgggat
     5461 ccggcggtga atttgcgaac agctccaagg gaacgaactt cgccattgct tcgtaacccg
     5521 caaccgacgg gtgcagatgg tcaccggagt cgaagcgtgc atccatcctt gccggatctt
     5581 cgggtgaacg cagtagtgcg tcgaaatcga tgacggcatc gaaatttccc ggcgtcctga
     5641 tccaggcgtt gactttctgc cgacttgcct ccgtaaggtc gtcggatgga tagaaggtgg
     5701 aaccccggaa cggtgtgatc gtcgcaccta tgatttcaat atccttctcg cgcgcccggg
     5761 ccaccatctg ctcatatgcg ccgatgattt gctggacgag attttcatgg tcgcgctgcg
     5821 tggcagattc gcttcgcgaa agcgtcccca ggtcgttcac gcccaaggca acgatgacct
     5881 gccgtgctcc tggcctgtcg agtacatcgc ggtcgaagcg ggcgagcccg ttaggtccaa
     5941 ggccatcgag cagcaggcga tttcctccca acccgacgtt caggacagcg agcgtgcctt
     6001 tcagcccggg atcctcccta atgcgagcga tcaggaagtc tgtccagcgc cggtcggtgt
     6061 tcggctgaac gccgaaccca tcggtgatcg agtcgcctat gaccagcagc gctcgcgatg
     6121 tgtcgcctgc ttccacatcg agaccggcga gctggtacca gtgatcgacc tgcctggctt
     6181 ccgggagatt ttctctctct acatggttgc cgcgttgtat gtaggacgtt gcgcgcgatc
     6241 ccgggtggcc cgtctgctgc gcgggagccg acggaaaata aatggacacg gccaggtgat
     6301 ccagtgccgc gaccttcatg ccgaccggat cggagaccat gtatgccccc gcgggaatgg
     6361 tcacgcgctc ctgccgtgaa aacgtcaccc gtgtcgacga aagaggctcg a
//
LOCUS       DASUOM010000613         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1535202,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000613 DASUOM010000000
VERSION     DASUOM010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535202"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="VFY27_12745"
     CDS             complement(<1..1707)
                     /locus_tag="VFY27_12745"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX5766432.1"
                     /translation="MKKQRATDGSLLASLPWTLAALAFSLTPHIQYLPVWITVSFLGC
                     AAVRWHIERQREHLPPAWLRVILALSGFIGVFASYESVSGVGPGSALLAVMAALKLLE
                     TRQRRDQFVLLFISIFLIMASLLREQHIWSLPFLVAGVILTATAWLRMSISDVLRIRD
                     SFGIAGRLLLYATPLALAMWIFFPRIATPFWSVPIDNGGATTGLSEEMSPGDISSLSQ
                     SDAVAFRVEFATQAPPPSQRYWRALVLHRFNGRTWSGNDPSIDLRAREEIQVSGEPVA
                     YEVTMEPSQQQWVFALDIPLQWSLKRTFMGSQQQLMSVQPIDRRVAYDAVSYLDYRTN
                     LDLSRMARSWYLRLPDSRNLRSAELARQMREAAASDADYVNDVLGMFRREEFFYTLQP
                     ALLGDNPVDRFLFETREGFCEHYASAFAVLMRAAGIPSRVVLGYQGGEMNPLGDYLIV
                     RQSDAHAWNEIWLEGRGWVRIDPTAAVAPERIEAGMSAARFGDAGARWGLTAPTRWLY
                     NLELTWDALNAKWNGWVLGYGPENQNRFMQWLGMDEPDWRKMVLTMLAILLLMIGALS
                     LLL"
     gene            complement(1704..>2433)
                     /locus_tag="VFY27_12750"
     CDS             complement(1704..>2433)
                     /locus_tag="VFY27_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5766433.1"
                     /translation="NLVDLEVSFAGVDPVFAGQAAEFRIAVTNHSKHRRHNIRLFAGS
                     VQSGVHDLKPGESRVLTLRMPTGRRGYRSLERYGIHTLFPFELFRAWAWLHMDLTALV
                     YPCPAEHAPLPPPTESAVGHRQYDVRGEEDFAGFRQFHDGDSPRHVAWKAYARSGEML
                     SKQFSGGDISSQWFDFNQVEARDTETRLSTLARWIIDAEQGRRNFGLRLPGRDIPPSH
                     GQAHRHRCLEALATFGDMRPVAET"
BASE COUNT          481 a          739 c          778 g          435 t
ORIGIN      
        1 gagcaggagg ctcaaagcgc cgatcatcag taggaggatg gccagcatcg tcagcaccat
       61 cttgcgccag tccggctcat ccatgccgag ccactgcatg aagcgattct ggttctcggg
      121 accgtacccc agtacccagc cgttccactt ggcatttagc gcatcccacg taagttccag
      181 gttgtacagc catcgcgtgg gggccgtgag gccccaccgc gcacccgcat ccccaaaacg
      241 cgcagcagac attcccgcct cgatccgttc cggcgcgacg gcagcagtgg gatcgatccg
      301 cacccagccc cggccttcca gccaaatttc gttccatgcg tgcgcgtccg actggcgaac
      361 gatcaggtaa tcgcccaggg gattcatctc gccgccctgg taaccgagca cgactcgcga
      421 gggaatcccc gcggcacgca tcagtacagc gaaggccgat gcgtagtgtt cgcaaaatcc
      481 ctctcgagtc tcaaaaagga agcgatcgac cggattgtca ccgagcaggg ccggctgcaa
      541 ggtgtagaaa aactcttccc gccgaaacat cccgagaaca tcgttgacgt agtccgcgtc
      601 gcttgcggca gcttcgcgca tctgccttgc cagttcggcg ctgcgcaggt tgcggctgtc
      661 gggcagccgc aaataccagg acctggccat ccgcgacagg tccaggtttg ttcgatagtc
      721 gaggtaggac accgcatcgt aggcgacgcg ccgatcgatc ggttgtacgc tcatcagctg
      781 ttgttgcgat cccatgaacg tgcgcttcag ggaccactgc agcggaatgt ccagtgcgaa
      841 cacccattgc tgctgcgacg gctccatggt gacttcgtag gctacgggtt cgccgctgac
      901 ttggatttct tcgcgggcgc gaaggtcgat ggaaggatca ttgccggacc atgtgcggcc
      961 gttgaagcgg tgcagcacga gcgcacgcca gtagcgctgg ctgggcggcg gcgcctgggt
     1021 cgcgaactcg acgcggaacg ccaccgcgtc ggactgcgac agggagctga tgtcgccggg
     1081 actcatctcc tcgctcaagc ctgttgttgc accaccatta tcgatcggca cggaccagaa
     1141 tggcgtggcg atacgtggaa agaaaatcca catggccagg gcgagcggtg tcgcgtaaag
     1201 gagcagtctt ccggcgatgc caaaactgtc gcggatccgc aagacatcgg agatcgacat
     1261 gcgcagccat gccgtcgccg tcagtatgac gccggccacg agaaaaggca gcgaccagat
     1321 gtgctgttcg cgaagcagcg atgccatgat caggaagatc gagataaaca gcagcacgaa
     1381 ctggtcacgg cgctgacgtg tctccaggag tttcagtgct gccatcactg caagaagtgc
     1441 agagcccggg ccgacaccgc tgacggattc gtaggacgcg aatacaccga tgaagccgga
     1501 caatgcaagt atcaccctga gccatgcggg gggaagatgc tcgcgctggc gttcgatatg
     1561 ccagcgtacc gctgcgcagc cgaggaaaga gacggtaatc cacaccggaa ggtactggat
     1621 gtgcggcgtg agcgagaacg ccagcgctgc cagtgtccag ggcaggcttg cgaggagcga
     1681 gccgtctgtg gcgcgttgct ttttcatgtc tcggccacgg gacgcatatc accgaaagtc
     1741 gcgagagctt caaggcagcg gtggcgatgc gcctgcccgt gtgagggcgg tatgtccctg
     1801 ccggggagac gcaggccgaa gttgcgccgg ccctgctcgg cgtcgatgat ccagcgcgca
     1861 agtgtggaaa gacgtgtttc ggtgtcccgg gcctcgacct ggttgaaatc gaaccactgg
     1921 ctgctgatgt cgccgccgga aaattgcttg gacagcattt caccgctccg cgcgtaagcc
     1981 ttccacgcca cgtgtcgcgg ggagtcgccg tcgtggaact gcctgaagcc tgcgaagtcc
     2041 tcctcgccgc gaacatcgta ttggcggtgg ccgaccgcgc tttccgtcgg cggcggtagc
     2101 ggtgcgtgtt cggcgggaca cgggtatacg agcgcggtga ggtccatgtg cagccatgcc
     2161 catgcgcgga acagctcgaa cgggaacagt gtgtgaatgc cgtagcgctc gaggctccgg
     2221 tagccgcgcc tcccggtcgg catccggagc gtcaggacac ggctctcacc gggtttgaga
     2281 tcgtggacgc cactctgcac ggagccggcg aatagccgga tgttgtgcct ccggtgcttc
     2341 gaatgattcg ttaccgcgat gcgaaactcc gctgcctgtc cggcgaatac gggatcgact
     2401 ccggcgaaac tgacttccag gtccacgagg ttt
//
LOCUS       DASUOM010000614         7098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_61258,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000614 DASUOM010000000
VERSION     DASUOM010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61258"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /gene="tig"
                     /locus_tag="VFY27_12755"
     CDS             complement(<1..1076)
                     /gene="tig"
                     /locus_tag="VFY27_12755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5766434.1"
                     /translation="MMVTVESTGKLERRMRVELPAERIEKEIETRLKKVGRTVRIKGF
                     RPGKIPSKVVRQRYGGQIRQEVLSELVQKSYSDAVIQENLNPAAGPRIVPEATGDGKD
                     FAYVATFEVLPEVKLKDLDKIEVEKPEVEISDEDREAMILNLRKQKASFEPVERAAQE
                     GDRVIVDFDGTLKGESVKGGKGKEVPVTLGQGQMLPDFEKALYGVKPGEEKTFKVKFP
                     KDYHAEELAGQKVEFAIKVHRVEEEKLPPLDDSLAELYGVEEGGLEQLRADVVSNMER
                     EAAEKVKTDVKEQVMNALLELNPIDIPSSLTHEEMHSRQHEAMRRLGIKDHDKAPPVE
                     NFRPGAERSVRLGLLMRQLIEDKK"
     gene            complement(1176..1260)
                     /locus_tag="VFY27_12760"
     tRNA            complement(1176..1260)
                     /locus_tag="VFY27_12760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1224..1226),aa:Leu,seq:tag)
     gene            complement(1524..1599)
                     /locus_tag="VFY27_12765"
     tRNA            complement(1524..1599)
                     /locus_tag="VFY27_12765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1564..1566),aa:Lys,seq:ctt)
     gene            complement(1660..1735)
                     /locus_tag="VFY27_12770"
     tRNA            complement(1660..1735)
                     /locus_tag="VFY27_12770"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1700..1702),aa:His,seq:gtg)
     gene            complement(1818..1894)
                     /locus_tag="VFY27_12775"
     tRNA            complement(1818..1894)
                     /locus_tag="VFY27_12775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1858..1860),aa:Arg,seq:tct)
     gene            complement(1925..2001)
                     /locus_tag="VFY27_12780"
     tRNA            complement(1925..2001)
                     /locus_tag="VFY27_12780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:Pro,seq:tgg)
     gene            2169..2780
                     /locus_tag="VFY27_12785"
     CDS             2169..2780
                     /locus_tag="VFY27_12785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5766435.1"
                     /translation="MFTHSSNAPAFLKVLLPVLLMLGCQAAVAADEVPATQHVRFQTS
                     EGDMVIELDGRRAPLTVGHVLELIDNGYYNGTIFHRVIPGFMIQGGGYTRDLKEKDAG
                     DEAIPNESGNGLQNLRGTVAMARLPDPHSANAQFYINVVDNASLNPRPDRWGYTVFGY
                     VIEGMDVADKIAATPTGPAGEFASDVPVATVVIEKASRVDYSP"
     gene            2798..3523
                     /locus_tag="VFY27_12790"
     CDS             2798..3523
                     /locus_tag="VFY27_12790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX5766436.1"
                     /translation="MTTLFISDLHLEGARPEIGTHFLEFLQREALLAEALYILGDLFE
                     SWIGDDDPNRHYAEMKAAIRGVADSGVPVFFMHGNRDFLIRERFAAEANVRLLPDPFQ
                     VGLYGEEVLLSHGDALCTDDLKYQEVRAVTRNPGWQAMMLKKPLEERQAIARQARADS
                     VAHGGSLDPQISDVNQGAVEQLLRTHGVRTLLHGHTHRPAVHRFQLDGALATRIVLGD
                     WYEQGSVVRWDAAGPKLSVLERR"
     gene            complement(3626..4219)
                     /locus_tag="VFY27_12795"
                     /pseudo
     CDS             complement(3626..4219)
                     /locus_tag="VFY27_12795"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
     gene            complement(4201..5445)
                     /locus_tag="VFY27_12800"
     CDS             complement(4201..5445)
                     /locus_tag="VFY27_12800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5766437.1"
                     /translation="MDVANGPRAMNIKVMIIDGQADFRTLLMHHVTTCWPDAIISDYD
                     PTSAGHLPEEFSGAGSDIVLLGDNQGNRSGIKTLKTYARKPEFPPIAYFCSNGEMKRE
                     AIAAGAQAVFLRTRIKHDELVVRIGHILRARRRVASTQSLFVGDSRTGIHPLVKGYTF
                     LKKLAASEHSAVYLAEKESTGNKMVLKVLRQVPDLSDGIGAFDRFLQEYEMIADLVHP
                     NIVKIHDLGVSDDHAHIAMEYLSGGDLKQRIQDGILEADAVRYAREIASALAQVHGVG
                     ILHRDLKPGNVMLREDGSIALIDFGLAKRMRLEQEMTGSGEIFGTPYYMSPEQGHADD
                     ADIRSDIYSLGVIFYEMLTGEKPYKASNAMGIIYKHSHAPIPILSPRLEKYQVLLDRV
                     LAKMPDDRLQSAEEVVEWLKRF"
     gene            complement(5436..7007)
                     /gene="purF"
                     /locus_tag="VFY27_12805"
     CDS             complement(5436..7007)
                     /gene="purF"
                     /locus_tag="VFY27_12805"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5766438.1"
                     /translation="MCGVVGIVGKGPVNQAIYDALTVLQHRGQDAAGIVTSGEAGLQV
                     RKGNGLVRDVFRQRHMLQLTGNVGLGHVRYPTAGGAQAAEAQPFYVNSPYGICLAHNG
                     NLTNFDELADLLTREDRRHLATGSDSEVLLNVFAHELQSRTSGHPTPEQIFDAVEAVH
                     RRCRGGYAVVAMIIGHGIVAFRDPNGIRPLVAGMRETGQGNEYMVASESVALDILRFR
                     KLRDLAPGETIFIENSGKLHSREHKGPVRHAPCIFEFVYFARPDSIIDNLSVYKARLR
                     MGEQLAGQIVREWPDHDIDVVIPIPDTSRTSAVQVAYHLGVKYREGFIKNRYIGRTFI
                     MPGQAERAQSVRRKLNAIDLEFRGKNVLLVDDSIVRGTTSKQIIRIARDAGARKVYFA
                     SAAPPVRYPNVYGIDMPAASELIANGRSVEEVQELIGADRLIYLDLHGLVRSVRHDNS
                     AITEFDTSCFSGEYITGDITPQYLAKLEVARNDKAKARNGNGRQARQAQAAPSPAEEA
                     APDKEAGRKDRAAWM"
BASE COUNT         1248 a         2239 c         2123 g         1488 t
ORIGIN      
        1 attttcttgt cctcgatcaa ctggcgcatc agcaagccca gccggacgct tctttccgcg
       61 cccggccgga agttctcgac aggtggcgcc ttgtcgtgat cctttatccc caaacgccgc
      121 atcgcctcgt gctgccgcga atgcatttcc tcgtgggtca gtgaactcgg gatgtcgatg
      181 gggttcagct ccagcaaggc attcatcacc tgctccttga catcggtctt cactttctcc
      241 gcagcttcgc gttccatatt gctaacgaca tcggcgcgca gttgctcgag cccgccttct
      301 tcaaccccgt acagctctgc aagactgtcg tccagcggag gcagcttttc ttcctccact
      361 cgatgtacct tgatcgcgaa ttccaccttc tgccccgcca gctcctctgc gtggtagtcc
      421 ttggggaatt tcaccttgaa ggttttttcc tcacccggct tcacgccgta aagcgccttc
      481 tcgaagtccg gcagcatttg cccctgcccg agcgtcaccg gaacctcctt gcccttgccg
      541 cccttcacgg actcgccctt cagcgtaccg tcgaagtcga caatgacccg gtcaccttcc
      601 tgcgcggcac gctccaccgg ctcgaagctt gctttctgct tccgcaggtt gaggatcatg
      661 gcttcgcgat cctcgtcgct gatctctacc tccggcttct ccacttcgat cttgtccagg
      721 tcctttagct tgacctcggg cagcacttcg aatgtggcga cataagcgaa atccttgccg
      781 tcgccagtgg cctcgggaac gatcctcggt cctgcggcag gattcaggtt ttcctggatc
      841 acggcgtcgc tgtagctctt ctgcaccagt tccgacagca cttcctgccg gatctgtccg
      901 ccatagcgct gacgcacgac cttggaaggg atctttcccg gccgaaaccc cttgatcctg
      961 accgtgcggc ctactttctt gagccgcgtc tcgatctctt tttcgatgcg ctcggccggc
     1021 agctcgacac gcatgcggcg ctcgagtttg ccagtcgact cgacagtgac catcatttcc
     1081 ctgacccttt tctcatttcc ggcagtggcg gcccgacttg ggccgagcct gcctctttct
     1141 catgttcaaa caagttaaat ggtagccaaa gaatttggtg cgaaaggaga gactcgaact
     1201 ctcacgggtt gccccacagg atcctaaatc cagcgcgtct accaattccg ccactttcgc
     1261 gcgagtttcg gctctttccc ttcaacccgt tgttcgaacg acgattttga cgttttgttc
     1321 acctgtgcct gtccgccagg cccgtcgata tcaagcccct ttgccgggct ggcgcgagta
     1381 taacgcgaaa catatggggg cagatgtgga ttatcccagc atgcggaccg cccgggaccg
     1441 cggatgcgcg ctttttccgc gccgacggcg gccgacgata agggggaagc gatgcgtgga
     1501 cgctggaacg gctcggactt gaatggtggg cccccgggga cttgaacccc gaaccaatgg
     1561 attaagagtc cactgctcta ccaattgagc tagaggcccc gcttcacgtc ttccccggct
     1621 gcccctcgac atggaggctt tatgcttttc tttaaaagat ggggtgagcg atgggattcg
     1681 aacccacgac gaccgggatc acaacccggg gctctaccaa ctgagctacg cccaccattt
     1741 gttgcgctat cttaccggaa ccagctctac aacaagcggt cgtggcgact tccggaataa
     1801 caaacctcgc agtcgactgg cgcgcccggc aggaatcgaa cctgcaaccc ccagcttaga
     1861 aggctggtgc tctatccgat tgagctacgg gcgcgttgtt tcgtagtcag ccggactccc
     1921 gggttggtcg gggcagacgg attcgaaccg ccgaccctct gctcccaaag cagatgcgct
     1981 accagactgc gccatgcccc gccgccggat ccactccgcg cgctgactcc ttatctcagg
     2041 aaccgccagc gcggcggggg cagaatgata cggccgcccc ggaagtcagt caattttcgt
     2101 tcccggtgga catttgccgg gggcgctcga taatagaatc acgttttact ggcaccacgg
     2161 actcctcaat gttcactcat tcatccaacg cccccgcttt tctgaaggtc ctgctaccgg
     2221 ttttgctgat gctgggctgc caggctgccg ttgccgccga tgaagtgccg gcgacccagc
     2281 atgtccgttt ccagacgagc gaaggggaca tggtgataga gctggacggc cgtcgcgcgc
     2341 ctctcacggt cggtcatgtg ctggaattga tcgataacgg ctactacaac ggcacgatat
     2401 tccaccgcgt tatcccggga ttcatgatcc agggtggcgg atacacccgt gatctgaaag
     2461 aaaaggacgc gggggatgag gcgattccca acgagtcagg taacgggctg caaaacctgc
     2521 gaggcacggt ggcgatggcc agacttccgg acccccacag tgcgaatgcg cagttctaca
     2581 tcaacgtcgt cgacaatgct tccctgaacc cgaggcctga ccgctgggga tacaccgtgt
     2641 tcggctacgt catcgaaggc atggacgtcg ccgacaagat tgccgccaca ccgaccggcc
     2701 ccgccggcga attcgcgagc gacgtcccgg tggcaactgt ggtcatcgag aaagcgtcgc
     2761 gagtggatta cagtccgtag ccgccggaga gagcagcttg accactctct tcatctccga
     2821 cctgcacctc gagggcgcac gtcccgagat agggacacac ttcctcgagt tcctgcagcg
     2881 ggaagcttta ctggccgaag cgctgtacat cctcggcgac ctgttcgaat cctggatcgg
     2941 tgacgacgac ccgaaccggc attacgcaga aatgaaggcg gccatccgcg gcgttgcaga
     3001 cagcggcgtg ccggtgttct tcatgcacgg caacagggat tttttgatcc gcgaacgctt
     3061 cgctgcggag gcgaacgtcc ggttgttgcc ggatcctttc caggtcgggc tttacggcga
     3121 agaggtattg ctgagtcacg gggacgcctt gtgcaccgat gacctcaaat accaggaagt
     3181 gcgggcggtt acgcggaacc ctggatggca ggcgatgatg ctgaagaagc cgctcgagga
     3241 gcgccaggcg atcgcgcggc aggcgcgcgc cgacagcgtg gcacatggcg ggtcgcttga
     3301 tccgcagatt tcggacgtca accagggagc cgtggagcaa ctgctcagga cgcatggcgt
     3361 caggaccttg ttgcatgggc acacacaccg gcctgccgtg caccgtttcc agctcgacgg
     3421 ggcgctggcg acgaggatcg tgctggggga ctggtacgag cagggaagcg tggtgcgctg
     3481 ggatgccgcg ggaccgaagc tgagcgtgct ggagcggcgg taggtggtgt tgcgggagcc
     3541 tgacatccag gcaggttcgc tgacgcacgg tcgcactcgc ccgcatggcg ggctcctgca
     3601 tgagcggggg accgctgtgg aagcggaact gcgggtcggg gtcgatgatt agcgccgcct
     3661 ctatcgactt gagttctgcc acgcgctttg cgatcgcgtc gtcgcttgtg gcctcgcttt
     3721 tcgcgaagtc gaggtactgt ccgaagtggc gggcctccga gacgagtaag cctttgtaga
     3781 acgttgccag gtccggcggc agctgcggga tcagggcggc gaagcgttcg caggagcgcg
     3841 cttcgatcag tgcgccgata atcagcgtat ccgtcaggcg atgcggttct gcagttctcg
     3901 ccgccgcacg caacgtagcg gcgtagcgcg atgcatccag gcgggaaaaa gggatgtccc
     3961 gctcgcgcat tagcttcttt acctgctcgt agtgcctcag ttcctctcgt gccagccgcg
     4021 acatccgttc cgcaagcagt ccgcgctccg ggtagcgata caggaaaccg atcgcggttg
     4081 acgccgcctt cagctcgcaa ttcgcgtgat cgagcaggag ttcgggtatg cgcccgcaag
     4141 cttcgtcaac ccaggcttgc ggagtcctgg tctggcaaaa atccgcaacg gccgcgggcg
     4201 tcagaaccgc ttcaaccatt cgactacctc ctccgcgctt tgcaggcggt cgtccggcat
     4261 cttggcgagc acgcggtcca gcaacacctg gtacttttcc agcctgggcg aaaggatcgg
     4321 gatcggcgcg tggctgtgct tgtagatgat ccccatcgcg ttcgaggcct tgtacggctt
     4381 ctccccggtc agcatctcgt agaagatgac gccaaggctg tagatgtcgc ttcgaatgtc
     4441 cgcgtcgtcg gcatgtccct gttcagggct catgtaataa ggagtgccaa aaatctcgcc
     4501 gctgcccgtc atctcctgct ccaggcgcat gcgcttcgcc aggccgaaat cgataagcgc
     4561 gatgctgccg tcttcccgca gcatgacgtt ccccggcttc aggtcgcgat gcaggatgcc
     4621 gacgccgtgc acctgcgcca gggcactggc gatctcgcgg gcgtagcgaa ccgcatccgc
     4681 ctccaggatg ccgtcctgga tccgctgctt caggtcaccg cccgacaggt actccatggc
     4741 gatgtgcgca tggtcgtcgc tcaccccgag atcatggatc ttcacgatgt tcgggtgcac
     4801 gaggtccgcg atcatctcgt attcctgcag gaaccggtca aaggcgccga tgccgtcgga
     4861 caggtccggg acctggcgca gcaccttcag gaccatcttg ttgccggtgg attccttttc
     4921 cgcgaggtac accgccgaat gctcgctggc cgccagcttt ttcaggaacg tatatccctt
     4981 cacgaggggg tggatgcccg tcctcgagtc gccgacgaaa agtgactggg tcgaagccac
     5041 gcgtcgccgg gcgcgcagga tatgcccgat ccggacgacc agttcgtcgt gttttatgcg
     5101 ggtacgcagg aataccgcct gggcgcctgc ggcgatcgcc tcgcgcttca tctcgccatt
     5161 gctgcagaag tacgcgatcg gcgggaattc cggctttcgc gcataagtct tgagcgtctt
     5221 gatgccgctg cggttgccct ggttgtcgcc aagcaggacg atgtcgctgc ccgcgccgct
     5281 gaattcttcc ggtaggtggc cggcggaagt cgggtcgtaa tcggagatga tggcgtcggg
     5341 ccagcaggtc gtgacgtgat gcatcagcag ggtgcggaag tcggcctgcc cgtcaatgat
     5401 catcaccttg atattcatgg cgcgcgggcc attcgctaca tccacgcagc cctgtctttc
     5461 ctgcccgctt ccttgtccgg ggccgcttct tccgctggcg atggcgctgc ctgtgcctgc
     5521 ctggcctgcc gcccgttccc gttcctggct tttgccttgt cgttccgggc aacctccagc
     5581 tttgcaaggt attgaggggt gatgtcgccg gtgatgtact cgcccgagaa acaggaagta
     5641 tcgaactcgg taatcgccga gttgtcgtgc cgcacggagc ggaccagccc gtgcaggtcg
     5701 aggtagatca gcctgtccgc accgataagt tcctgcactt cctcgaccga ccggccgttc
     5761 gcgatcagct cagaagcagc cggcatgtcg atgccgtaga cgttggggta gcgcaccggc
     5821 ggcgcggcgg aggcgaagta caccttgcgc gcgccggcgt cgcgggcaat ccggatgatc
     5881 tgtttggagg tcgtcccccg tacaatcgag tcgtcgacca gcaggacgtt cttcccgcgg
     5941 aattcgagat cgatggcgtt cagcttgcgc cgtaccgatt gcgcgcgctc cgcctggccg
     6001 ggcatgatga atgtccgccc gatgtagcgg ttcttgataa agccttcccg gtacttcacc
     6061 cccaggtggt acgccacctg caccgccgag gtccggctgg tgtcgggtat gggaataacg
     6121 acatcgatgt cgtggtccgg ccattcacgg actatctggc ccgcaagctg ctcccccatg
     6181 cgcaacctgg ccttgtaaac cgacaggttg tcgatgatcg aatcgggccg cgcgaaatac
     6241 acgaattcga agatgcaggg cgcgtgccgt accggtccct tgtgttcccg gctgtgcagt
     6301 ttgccgctgt tctcgatgaa aatggtctcg ccgggggcga ggtcgcgcag cttgcggaag
     6361 cgaagaatgt cgagcgcgac gctctcggaa gccaccatgt actcgttgcc ctgtccggtc
     6421 tcgcgcatgc ctgcaaccag cggccggatg ccgttcggat cgcgaaaagc aacgattccg
     6481 tgaccgatga tcatcgcgac caccgcgtag ccgcccctgc agcgacgatg caccgcctcg
     6541 actgcgtcga atatctgttc gggtgtcggg tgcccgctgg tgcggctctg caactcgtgg
     6601 gcaaaaacgt tcagcagcac ttccgaatct gaaccggtcg cgaggtggcg acggtcttcc
     6661 ctcgtcagga ggtctgcaag ttcatcgaag ttcgtgaggt tgccgttgtg tgccaggcag
     6721 atgccatatg gcgagttcac gtagaacggc tgtgcctcgg cggcctgtgc gcctccggcg
     6781 gtcgggtagc gcacgtggcc cagaccgaca ttgccggtca gctgcagcat atggcgctgg
     6841 cggaacacat cccgaaccag gccgttgcct ttcctgacct gcagtcctgc ttcgcctgag
     6901 gtgacgatgc cggccgcgtc ctggccccga tgctgcaaca ccgtcaaggc atcgtagatc
     6961 gcctggttga caggcccctt cccgactatc ccgacaacac cacacattat tgaaagcttc
     7021 gccctgcgcg cattcagacc ggcccatcga ctgcagaatc ggccggctct tcgtccttct
     7081 gcagcatatc aacgcctt
//
LOCUS       DASUOM010000615         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1415863,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000615 DASUOM010000000
VERSION     DASUOM010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415863"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFY27_12810"
     CDS             <1..181
                     /locus_tag="VFY27_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5766439.1"
                     /translation="PVSTTHTLVGAILGVGMARGIDAIDLRVVGRILASWLITVPIGA
                     LLSIIFFFFFKTVLP"
     gene            194..1498
                     /locus_tag="VFY27_12815"
     CDS             194..1498
                     /locus_tag="VFY27_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766440.1"
                     /translation="MPVEQAATRTAVQARSLATRRYVLALLVVVYTFNFIDRQILAIL
                     LPAIKAEFGVNDTVLGFLAGSAFALFYATLGVPIALLADRWNRRNLIALSLAIWSGMT
                     ALSGVATNIVQLTLARIGVGVGEAGCSPPAHSIISDLYSPQERSTAMGIFTLGISAGI
                     MIAYLAGGWVAENIGWREAFLIVGLPGLALALLVRFTISEPVRGQSDGKKDSAARPGI
                     VAVARFLLGRKSFLHLAVGSGLAAFIGYAVISFFPTFLFRSHGMNVTEIGVWLGIILG
                     TAGGLGFAGGGYVADRLGRRGQRYALWGVTAATMFAWLFHLPVFLVGSPYLALALFVI
                     PAIFANFYLATTFAQIQGLVGLRMRAVASALLLFILNSVGLGLGPLSAGVLSDLLEGF
                     FGGDSMRYSLLFIALVVGPWSAWHYFAAGRHIEGDLARSDET"
     gene            complement(1521..2912)
                     /locus_tag="VFY27_12820"
     CDS             complement(1521..2912)
                     /locus_tag="VFY27_12820"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5766441.1"
                     /translation="MARRNSVARNPELRAFLKEFPDTDVMELLIADLSGVHRSKRIRG
                     SEFEKTFSSGFYIPGGAVLLDVLGNVVSGISFTADDGDPDVDARVVPGSLAPIPWAKR
                     PSAQALFRLYARSGEPFFGDPRYVLERAAEPLRAMKLKIVMAAELEFYLLDAKADRPR
                     ARVSRIPGIGRPQVGPQVYHPDDLWDIDNFLHDINDACRAQNIPVGTTTSEFAPGQFE
                     INLQHVADPVLACDHAVLLKRVVKSVARERGLVACFMAKPFEESAGSGLHIHLSLIDR
                     EGRNYFSGGKERAATPPFSARFRHAVGGLAHTMAEATAIFAPNANSYRRLRPDMFAPV
                     EPNWGANHRNVSLRVPVSNADNLRIEHRTSGADANPYLVTAAILAGVHYGIKNRCEPG
                     RMVREGEVIDLKRKLPARWEAAIEKFAHSRILPEYLGSEYCKAYAINRRVEAERFHNT
                     VSDVDFDWYMRAV"
     gene            complement(2958..3770)
                     /locus_tag="VFY27_12825"
     CDS             complement(2958..3770)
                     /locus_tag="VFY27_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5766442.1"
                     /translation="MSTIQVENLGDVARITLNRPEKLNAIDSGMLEQLEQALDAAEQQ
                     EDVRAILLDGNGRAFSAGFDIDTEVPAESREDHLRRELTRDFEVIMRFWDCPKPIVAA
                     VHGYCLGSSMEIAAVCDVTIAADDSRFGAPEVRYGSGIVCLVLPWIVGYKNASELLLA
                     GDNNIDAARAAGIGLVNRVVPAAMLHSEALAMARQISLNDALAVRLTKRAIHRSMDIA
                     GMRQALHEALEVDIEIETTDTEESIAFNEILREQGVKAALAWRARNATNAAG"
     gene            complement(3772..4860)
                     /locus_tag="VFY27_12830"
     CDS             complement(3772..4860)
                     /locus_tag="VFY27_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5766443.1"
                     /translation="MEATLQRDLYIEPSWFAREREKIFLDQWTCVAREEDLVAAGSYE
                     AVKIAGESIIVVRGDDWKLRAFHNVCRHRGCELIDSTSDAQKRGQFLANIRCPYHSWT
                     YRLDGKLHHTPYVEVEKEKFGLHEVALDCWGGLVFVKVGTGSQTLREQLGPVPGRVVR
                     YPLEELGTGWRKEYLVAANWKVILENYNECYHCAGVHPELCKIVPEFRRNGGAQLDWE
                     AGIPQKPGTNTFTRTGTTSRLPFPGLNAAEKERHFGELAYPNLMLSLSMDHVAMFILW
                     PQGPEQTRIDCRLLFHPEAINRMDFDPSDAADLWDLVNMQDWDICERVQRGMHARPFR
                     HGLYAPMEDLSLDIRNYVAARLGSLPID"
     gene            4976..>5223
                     /locus_tag="VFY27_12835"
     CDS             4976..>5223
                     /locus_tag="VFY27_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5766444.1"
                     /translation="MFLSRRYDALYDWSHKQREVATTGTVVMSAEAKSNRPFDAIVIG
                     AGHNGLTTAFYLARSGLRVLVLERRDVVGGCAVTEEIDA"
BASE COUNT          888 a         1620 c         1587 g         1128 t
ORIGIN      
        1 accggtatcg accacccata ccctggtcgg ggcgatactc ggtgtgggga tggcccgtgg
       61 aatcgatgca atagacttgc gggttgttgg ccgcattctc gcttcatggc tcataacggt
      121 tcctatcggc gcgctgctct ccatcatctt cttcttcttt ttcaaaaccg tgttgccctg
      181 agatcgacgc atgatgcccg tagagcaagc tgcaacccgt acggctgtgc aggcgcgctc
      241 cctcgccaca cgtcgatacg tgctggcgct tctcgtcgtg gtctacacgt tcaacttcat
      301 cgaccggcag atcctggcga tcctcttgcc ggcgatcaaa gcagagttcg gcgtcaacga
      361 tacggttctg ggcttccttg ccggctccgc gtttgcgctg ttttacgcga ccctgggcgt
      421 gccgattgcg cttctcgccg atcgctggaa tcgccgtaac ctcatcgctc tctcgctggc
      481 gatctggagt ggaatgaccg ccttgtccgg cgtcgccacc aacatcgtgc agctgacgct
      541 cgcgcgcatc ggcgtgggag tgggtgaagc cgggtgcagc ccacctgcgc attccattat
      601 ctccgacctg tactcgccgc aggaacgatc cacggcgatg ggaattttca ccctgggaat
      661 ttccgcgggc atcatgatcg cgtacctggc cggcggctgg gtcgccgaga acatcggctg
      721 gcgcgaggcg ttcctcattg tcggtctgcc ggggctggcg cttgcgctac tggtccgttt
      781 cacgatcagc gaacccgttc gcggccagtc cgacggcaag aaagacagcg cggcgagacc
      841 cggtatagtg gcggtggccc ggttcctgct cggacgaaaa tcgttcctgc accttgccgt
      901 gggatcagga cttgcggcct tcattggcta cgccgtgatc agcttcttcc caacgttcct
      961 gttccgttcg cacgggatga acgtgacgga aatcggcgtc tggctcggca tcatcctcgg
     1021 tacggcggga ggcctggggt tcgcgggagg aggctatgtc gccgacaggc tcgggcgccg
     1081 cggccagcga tacgcgttgt ggggcgtgac tgccgcaacg atgttcgcat ggctttttca
     1141 cttgcctgtg ttcctggtcg gaagccctta cctggcgctg gccctgttcg tcattccggc
     1201 catcttcgcg aacttctacc ttgcgacgac cttcgcacag atccagggcc tggtgggcct
     1261 gcggatgcgc gctgtagcgt ccgctttgct gctgttcatc ctgaactcgg tgggactggg
     1321 gctgggaccg ctgtccgcag gtgtgttgag cgacctgctg gagggctttt tcggcggcga
     1381 ctcaatgcgc tacagcctgc tgttcatcgc cctggtcgtc ggtccctggt cggcgtggca
     1441 ttatttcgca gccggccggc acatcgaagg cgatctcgcc cgctccgacg agacctgatt
     1501 catccacatg ccgtcgccgc ttacaccgcg cgcatgtacc agtcgaaatc cacgtcgctg
     1561 acagtgttgt ggaagcgctc tgcctcgacg cgccggttta tcgcataagc cttgcagtat
     1621 tcgctcccga ggtactccgg cagtatgcgg ctatgcgcga atttttctat ggcggcctcc
     1681 cagcgtgcgg gtagcttgcg cttgaggtcg ataacctcac cttcccgcac catccttccc
     1741 ggttcgcagc ggttctttat tccgtagtgc acgcccgcca atatcgcggc ggtcacgaga
     1801 tacggatttg cgtctgcgcc cgaagtgcga tgctcaatcc gcaggttgtc cgcgttggac
     1861 accggcacgc gcaacgaaac gttgcggtgg ttcgcgcccc agttcggttc caccggcgcg
     1921 aacatgtcgg gacgcaggcg tcgataggag ttggcgttcg gcgcgaagat tgccgtagcc
     1981 tccgccatcg tatgtgcgag cccgcctact gcgtgcctga acctcgcgct gaaaggcggc
     2041 gttgccgcac gttccttgcc gcctgagaaa taattgcggc cttcgcggtc gatgagactc
     2101 aggtgaatgt gcaggccgga tcctgcgctc tcctcgaaag gcttggccat gaaacaggca
     2161 accaggccgc gctcgcgcgc cactgacttc acaacgcgct tcagcagcac ggcgtggtcg
     2221 caggcaagca ccgggtcggc gacgtgctgc aggttgatct cgaactggcc cggcgcgaac
     2281 tccgaggtgg tcgtgccaac cgggatgttc tgcgcgcggc atgcatcgtt gatgtcgtgc
     2341 aggaaattgt cgatatccca gagatcgtcc gggtgataca cctgcgggcc gacctgcggc
     2401 cgtccgatcc cgggtatcct ggacaccctg gcccttggac gatcggcttt cgcgtcgaga
     2461 agatagaatt cgagttctgc cgccatcacg attttgagct tcattgcgcg cagcggctcg
     2521 gctgcgcgtt cgagcacgta gcgggggtcg ccgaaaaacg gttctccgga tcgcgcgtag
     2581 agacggaaga gcgcctgggc actcggccgc ttcgcccacg gaatgggcgc aaggctgccc
     2641 ggcactaccc gcgcatccac gtccgggtcg ccgtcgtccg cagtgaagct gatgcccgaa
     2701 acgacgttac ccaggacgtc gagaagcacg gcgccgcccg ggatataaaa gccgctgctg
     2761 aatgtcttct cgaactcgct gccgcgtatg cgtttgctgc ggtgcacgcc cgagaggtcc
     2821 gcgatcagca gctccataac gtcggtgtcc gggaattctt tgaggaacgc acgcagttcc
     2881 gggttgcgag cgacgctgtt gcgtcttgcc atgccttcag gttccctttg gtcttgcgcc
     2941 ggtttgtgaa cgttgcttca tcctgccgcg ttcgtcgcgt tccgggcgcg ccatgccagg
     3001 gccgccttca cgccctgttc ccggaggatt tcgttgaacg cgatggattc ttccgtatcg
     3061 gtcgtttcta tctcgatgtc cacttcaagc gcttcatgca gtgcctggcg catgcccgca
     3121 atgtccatgc tcctgtggat cgcacgcttc gtcagccgca ccgcgagcgc atcgttcaaa
     3181 gagatctgcc gcgccattgc gagcgcttcg ctgtgaagca tggcggcagg cactacgcga
     3241 ttgacgagcc cgatgccggc agcgcgtgcg gcatcgatgt tgttgtcgcc tgccaggagc
     3301 agctcgctag cgttcttgta accgacaatc caggggagaa cgaggcacac gatgccgctg
     3361 ccgtacctca cctcgggagc gccgaagcgg ctgtcatcgg ccgcaatggt gacgtcgcac
     3421 acggccgcga tctccatgga actgcccagg cagtagccat gtactgcggc aacgatcggc
     3481 ttgggacagt cccagaaccg catgattact tcgaaatccc tcgtcagctc gcggcgcagg
     3541 tgatcttccc gcgattccgc gggaacctca gtgtcgatat cgaatcccgc ggagaaagcc
     3601 cggccattgc cgtccagcag aattgcccgc acgtcttcct gttgctcggc cgcatcaagc
     3661 gcctgctcga gctgctcgag catccccgaa tcgatcgcgt tcagtttctc cggccggttg
     3721 agcgtgatgc gtgcgacgtc gccgagattc tcgacttgaa tcgtggacat ctcagtcgat
     3781 cggtaggctg cccagtcggg cggctacgta gttgcgaata tcgaggctca ggtcctccat
     3841 cggcgcgtac agcccgtgcc tgaacggccg cgcgtgcatg ccgcgctgca ctcgttcgca
     3901 gatgtcccag tcctgcatat tcacaaggtc ccagagatcc gcggcatccg atggatcgaa
     3961 gtccatgcgg ttgatggctt ccgggtggaa cagcaggcga caatcgatgc gcgtctgttc
     4021 cggaccctgc ggccagagta tgaacatggc aacgtgatcc atggagaggc tcagcatcag
     4081 gttcggatag gcgagttcac cgaaatgccg ctccttctcg gcagcgttca gcccgggaaa
     4141 cgggagccgg ctggtcgtgc cggtgcgcgt gaacgtgttg gtccccggct tctgcgggat
     4201 gcctgcttcc caatcgagtt gcgctccgcc gttcctgcga aactcgggga cgatcttgca
     4261 gagctccgga tggaccccgg cgcagtggta gcactcgttg taattctcga gtatcacctt
     4321 ccagttggcc gcgacgagat attcctttct ccagcccgta ccgagttcct cgagcggata
     4381 tctcactacg cggccgggca ccggtcccag ctgctcgcga agtgtctggg aacctgtacc
     4441 gactttcacg aaaacgagtc cgccccaaca gtcgagcgcg acttcatgca agccgaactt
     4501 ttccttctcg acctcgacat agggtgtgtg gtgcagcttg ccgtccagcc ggtatgtcca
     4561 ggaatggtat gggcagcgga tgttcgccag gaactggccg cgcttttgtg cgtcggaagt
     4621 cgagtctatg agttcacagc cgcgatggcg gcaaacattg tgaaatgcac gcagcttcca
     4681 gtcgtcaccg cgcaccacga taatgctctc accagcgatc ttgaccgctt cgtagctacc
     4741 tgccgctacg agatcctctt cccttgccac gcaggtccac tggtcgagaa aaatcttctc
     4801 gcgttccctg gcaaaccatg agggctcgat gtaaagatcg cgctgaagcg tcgcttccat
     4861 ttcttttgta agagtccggc tgggccaagt gtgggtcttc gattcctgtt cgaagcctag
     4921 catagcgacc gagatatccg catcggagcg gtttcacatc gtggccaggt agccggtgtt
     4981 cctgagcagg cgttacgatg cgctttacga ctggtcacat aagcaacggg aagttgcaac
     5041 caccggcacg gtagtgatgt ctgcggaagc aaaaagcaac agaccttttg acgcaatcgt
     5101 catcggagca gggcacaacg gcctcacaac cgcgttctat ttggcgaggt cgggactgcg
     5161 cgtcctcgtg ctggaacggc gtgacgtagt gggcggttgt gcggtcaccg aagaaatcga
     5221 tgc
//
LOCUS       DASUOM010000616         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1179103,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000616 DASUOM010000000
VERSION     DASUOM010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179103"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFY27_12840"
     CDS             <1..1220
                     /locus_tag="VFY27_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766445.1"
                     /translation="LTPVPQVITPRSEETSQSDGPFKTPISAERSPAGVTNTTSEATA
                     PKIAESAASSAPVKSPAAPGESWSGLTWLGGSGVALILGLLLFGRKLKSRLAPDAFDR
                     RRTDGRSLDETDIAPIDGLPSQGTAPVARMVSLDADLDDGSGFQYGGDIDVAQDFGFS
                     ETSGKFPNRRDMDATAAHGGTDVIATRRVEEATILVSETPPQHEDSGEYDVSMIVDAT
                     KQMVEDQDGTAKDLGAIEVDAEEDDTLAEEYTLSKNVDYTILEQDYEDELTATQALNA
                     EIARAARALSRQFGKDEMGDTVTDTMNDEMDATAAAAFGEAFDDVFDDKTSQLPARDE
                     AGPDDATTALPAAGSGYPESDETTQLSVSSTMTLDDTANEEIALEVPAAENDPSVEVE
                     IESAMVDTKKMRA"
     gene            complement(1242..3485)
                     /gene="parC"
                     /locus_tag="VFY27_12845"
     CDS             complement(1242..3485)
                     /gene="parC"
                     /locus_tag="VFY27_12845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5766446.1"
                     /translation="MQNQLNLEGIEQLALKDYTEKAYLDYSMYVILDRALPQIGDGLK
                     PVQRRIIYAMSELGLSAGAKAKKSARTIGDVIGKYHPHGDSACYEAMVLMAQPFSYRY
                     AIVDGQGNFGSTDDPKSFAAMRYTESRLMPYAKSLLAELAHGTVDWTPNFDGTLDEPV
                     VLPARLPNLLLNGTTGIAVGLSTDVPPHNLHEVANALVHLIDNPKATVAHLMKHIKGP
                     DFPTGGELISPRADIKQIYETGNGTLRLRASYKVENGDIVINSLPYQVSGSKVLEQIA
                     VQMRNKKLPIVEDLRDESDHEEPIRLVITPKSKKIDVDELMSHLFSTTSLERTVRVNM
                     NIVALDGRPRIFNLPGLMTEWLKFRNATVKRRLNHRLQIVSDRLHILDGLLIAYLNID
                     EVIAIIRREDEPKPKLQKRFRLTEIQAEAILNLRLRNLAKLEEMKIRGEQDELNNEKK
                     DIEKTLKSGARLKALIKKEILEDAETYGDARRTKLVEREAAQALDETLLVASEPVTLV
                     LSRRGWARAAKGHEVDPLALSYKSGDEFLAAAQGRSNQLAMFVDSTGRVYSVPAHTLP
                     SARGQGEPLSGRFNPPDGAVFCGTMIGDADSKWLLASDAGYGFIAKLADLASRNKAGK
                     SVLRVPAGGQVVVPAPVPPDAECYIAAVSSIGRLLFFEMEELPELAKGKGNKIINIPG
                     KKYQAGEEKLVAVAVVPEGGNLQVQTNTRSMTIKWDDLDDYYGERGRRGALLAKGWRK
                     VDRLAAA"
BASE COUNT          707 a         1192 c         1153 g          752 t
ORIGIN      
        1 gtctgacgcc tgtgccgcag gttataaccc cgcgtagcga agagacatcg cagtccgacg
       61 gtccgttcaa aacgccgatc agtgctgaac gttctcctgc cggagtgacc aataccacat
      121 ccgaagcgac cgcaccgaag atagccgaaa gcgctgcgag cagcgcaccg gtgaaaagcc
      181 cggctgcccc cggcgaatcc tggtccgggc tcacctggct cggtggaagc ggcgttgccc
      241 tgatcctcgg actgttgctg ttcggccgga aactgaaatc ccggctcgcg cccgacgcat
      301 tcgaccggcg ccggaccgat ggcaggagcc tggacgaaac cgacatcgca ccgatagatg
      361 ggctaccgtc gcagggaacg gctcccgtgg cgcgcatggt gagcctggat gccgacctcg
      421 acgacggctc cggcttccag tacggcggcg acatcgacgt tgcgcaggat ttcggctttt
      481 ccgaaacttc cggcaaattc cccaaccgca gggacatgga cgcgacagcc gctcacggcg
      541 gcacggatgt cattgccacc aggcgtgtgg aggaggcgac catccttgtc agcgagactc
      601 cgccacagca tgaagacagc ggcgagtacg acgtatccat gatcgtcgat gcaacgaagc
      661 agatggtcga agatcaggac ggcacggcca aggatctggg ggccatcgaa gtcgatgccg
      721 aagaagacga caccctcgcc gaagaatata cgctcagcaa aaacgtcgac tacacgattc
      781 tggagcagga ttacgaagac gaactgactg caacccaggc tcttaacgcg gagattgcgc
      841 gggcagcgcg cgccctgtcg aggcaattcg gcaaggacga gatgggagac accgtcaccg
      901 atacgatgaa cgacgaaatg gatgcgaccg cggccgcagc cttcggggaa gcgttcgatg
      961 acgtcttcga cgacaagaca tcccagcttc cggcccgcga cgaagctgga ccggatgatg
     1021 caaccacggc cttgccggct gcgggttcgg gttacccgga gagcgatgag accacgcaac
     1081 tctccgtcag ctcgacgatg acgctcgacg acaccgcgaa cgaagaaatc gcgctggaag
     1141 tgccggcggc ggaaaatgat ccatccgtcg aggtggaaat cgaatccgcc atggtcgaca
     1201 cgaaaaagat gcgcgcctga cggacgggtc cttcgggggg ctcaagcggc agcgaggcga
     1261 tccaccttgc gccaaccttt cgccagcagg gcgccgcggc gcccccgttc gccgtagtag
     1321 tcgtcgagat cgtcccattt gatcgtcatc gatcgcgtgt tcgtctgtac ctgaaggttg
     1381 ccgccttccg gtacaaccgc cacggcaacg agtttctctt ccccggcctg gtatttcttg
     1441 ccggggatgt tgatgatctt gttcccctta cccttcgcca gctccggtag ctcttccatc
     1501 tcgaagaaca acagcctgcc aatggagctc accgctgcga tatagcactc ggcatccggt
     1561 ggaacaggcg ccggtaccac gacttgtcct cctgcaggca ccctcaggac cgatttcccg
     1621 gccttgttgc gcgaggcgag atccgcgagc ttggcaatga agccatagcc ggcatcgctc
     1681 gccaggagcc acttcgagtc ggcatcgccg atcatcgttc cgcagaacac cgcgccgtcg
     1741 ggcgggttga agcgaccgga cagcggctcc ccctgcccgc gcgccgatgg cagcgtgtgt
     1801 gccggcacgc tgtagacgcg gccggtactg tccacgaaca tcgcaagctg gttgctgcgg
     1861 ccctgcgccg ccgccaggaa ctcgtctccg gacttgtagc tcagtgcgag cgggtcgact
     1921 tcatgcccct tcgccgcgcg tgcccagccg cgcctggaca gtacgagtgt taccggctcg
     1981 ctcgcaacaa gcagggtctc atccagcgcc tgcgccgctt cccgctcgac gagcttcgtt
     2041 cgccttgcat cgccgtatgt ttccgcgtcc tcgagaattt ccttcttgat gagcgctttc
     2101 aggcgcgcgc cgctcttcag ggtcttctcg atatccttct tctcgttgtt cagctcgtcc
     2161 tgctcgccgc ggatcttcat ttcctcgagc ttcgccaggt tcctgaggcg caggttcagg
     2221 atcgcctccg cctggatttc cgtcagcctg aatcgcttct gcagcttagg cttcggctca
     2281 tcctccctgc ggatgatcgc gatgacttca tcgatgttca ggtaggcgat cagcaagccg
     2341 tcgaggatgt gcaggcggtc ggaaacgatc tgcagccggt gattcaggcg gcgcttcacg
     2401 gtggcgttgc ggaacttgag ccactccgtc atgagccccg gcaggttgaa gatccgcggc
     2461 cggccgtcga gcgccacgat gttcatgttg acccgtaccg ttcgctccag ggatgtcgtt
     2521 gagaacaggt gggacatcag ttcgtcgacg tctatttttt tcgatttcgg cgtaataacc
     2581 agccgtatcg gctcttcatg gtcagattca tcgcgaaggt cttcgacgat cggcagtttc
     2641 ttgttgcgca tctgcaccgc gatctgctcg agcaccttgc tgccggaaac ctggtacggc
     2701 agcgagttga tcacgatgtc gccgttctcc accttgtagg atgcgcgcag ccgcagcgtg
     2761 ccgttacccg tttcgtagat ctgcttgatg tcggctcgcg gcgagatcag ttcaccgccg
     2821 gtcgggaagt cgggtccctt gatgtgtttc atcaggtgcg cgaccgttgc cttcgggttg
     2881 tcgatcaggt gcaccagtgc attggcaacc tcgtgcaggt tgtgcggcgg cacatcggtc
     2941 gagagcccga ccgcgatgcc ggtcgtgcca ttcaggagca ggttgggcag gcgcgcgggc
     3001 agcacgaccg gctcgtccag ggtgccgtcg aagttcggcg tccagtcgac agtgccatgc
     3061 gcaagttcgg ccagcaggct cttcgcatat ggcatgagcc gcgattcggt gtaacgcatg
     3121 gcggcaaagg acttcgggtc gtcggtagag ccgaagtttc cctgcccgtc gactatggca
     3181 tagcgatacg aaaatggctg cgccatcagc accattgcct cgtagcaggc ggagtcgcca
     3241 tgcggatggt atttgccgat tacatcgccg atggtgcgcg ccgacttctt cgccttggcg
     3301 ccggccgaga gccccagctc gctcatcgcg tagatgatgc gccgctgcac cggcttcagc
     3361 ccgtcgccga tctgcggcaa cgcgcggtcc agtatcacgt acatcgaata gtcgaggtac
     3421 gccttctccg tatagtcctt aagggcgagt tgctcgatgc cctcgaggtt cagctggttt
     3481 tgcattcctg ttcctgtcat ttgctgtgcg caagaggaga tcggtggtcg ctgacagaca
     3541 gcagcacgca cggcgcatga acgcgatacg tttcggcgga gcctcggttg gggggagggc
     3601 ttgtcaggac tgtcgcaaac atggatgttt gcgcagagcc cccatggatg ggtttacggc
     3661 gttcctgaca agccctcccc ccagccgagg ttccgccaac gactccctct attcgttcat
     3721 gcgccgtgcg tactgccgtc tgtcagcgac caccgatctc ctcttgcgca cagcaaatga
     3781 caggaacagg aatgcaaaac cagc
//
LOCUS       DASUOM010000617         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1554121,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000617 DASUOM010000000
VERSION     DASUOM010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554121"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..2042
                     /gene="argS"
                     /locus_tag="VFY27_12850"
     CDS             291..2042
                     /gene="argS"
                     /locus_tag="VFY27_12850"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5766447.1"
                     /translation="MSQGVVQEKVASALLGALNGAREKGQLKTAAWPTLSLDAPKRPE
                     WGDLASTVAMSLASSEQRTPHDIAQIIVENLPQRDLLFDRVEIVRPGFLNLTVKPALW
                     QETLREIEAQGASYGKSDVGKGRRVLVEYVSANPTGPLHVGHGRGAAVGQALASLLHT
                     VGFEVVSEYYINDAGRQMKLLGASVTARYRELAGQPAAFPEDGYHGAYVKTLAERLKP
                     QLDAQSSLPPEQFEERVLTLAYRELLALIRDDLKAFGLEFSSWFSEASLLSSQGVDRV
                     LDELKSRQLLFQQDGAWWFRSTAFGDEKDRVVKKQDGEYTYLASDIAYHRDKLQRGYD
                     LLIDVWGADHHGYIPRMQAVMQAYGHPKERLQVILVQLVKLLRAGEEVKMSKRTGEFI
                     TMREVIDEVGADAAKFYFLMRDSKTHLEFDLELAKQRSADNPVYYVQYAHARIASLWR
                     VAASRGIACPLPSQTDLSLLSDPDELALIRKLAAYPSVLEAAAIGYEPHRMTYYLQQL
                     AALLHTFYNKHRILPTAADPEVFEQSPSGMTADEGAKRETLSPERTAARLALMRGVQQ
                     VIKNGLGVLGISAPDQM"
BASE COUNT          431 a          608 c          622 g          402 t
ORIGIN      
        1 tggaattatc gtctcaggcc attctcgacg cctgcaggac cacccgagcc gtcgcctaac
       61 ccctcgaatt tgctgagtca tgccttgtct ctctcttcag ccctgtggta cagtgccgag
      121 ttaattttct ctttcaatgc taaggactta cgcacattgc ccgtgacggt gtgtcgcgga
      181 acatgaagga cccaggcgtc attcgacagg acgttaaagg gggcgtaccg gtttgttaca
      241 acggcgaatc cgagtagaac cgttcatgga tcagatgcat taggagccat gtgtcgcagg
      301 gtgtcgtgca agaaaaggtg gccagtgcac tcctgggcgc cctcaatggg gccagggaaa
      361 agggccaact gaaaacggcg gcctggccca cgctgagcct cgatgcgccg aagcgtccgg
      421 aatggggcga cctcgcgtcg actgtcgcca tgtcgctggc gtcatctgaa caacgaacgc
      481 ctcatgacat cgcgcagatc atcgtcgaga atcttccaca gcgggacctg ctgttcgatc
      541 gggtcgagat cgtccggccg ggctttttga atctcaccgt caagccggcg ctatggcagg
      601 aaacgctgag agaaatcgag gcacaaggcg cttcctacgg caaaagcgat gtcgggaagg
      661 gacggcgggt gctggtggaa tatgtgagcg cgaatccgac cggcccgttg cacgtcgggc
      721 atggacgcgg cgccgctgtc ggacaagccc tcgccagtct gctgcacacg gtcggcttcg
      781 aggtcgtgag cgaatattac atcaacgatg ccggccgcca gatgaagctg ctgggcgcat
      841 cggtgactgc ccgctatcga gagctggcgg gccaacccgc tgcctttccc gaagacggct
      901 atcacggcgc ctacgtgaag acgttggcgg aacgcctgaa gccccagctg gatgcgcaga
      961 gcagcctgcc gcccgagcaa ttcgaggaac gggtcctcac gctggcgtat cgcgaactgt
     1021 tggctctcat tcgcgacgat ctgaaagcct tcggactaga gttttcctcg tggttcagcg
     1081 aagcctcgct gctctcctca caaggcgtcg accgcgtgct ggatgagtta aagtcccgcc
     1141 agctgctgtt tcagcaggac ggcgcctggt ggtttcgctc gacggccttt ggagacgaga
     1201 aagaccgcgt cgtcaaaaag caggacggcg aatatacgta cctcgcttcc gacatcgcct
     1261 atcaccgcga caagctgcag cgtggctatg atctgctaat cgatgtgtgg ggtgccgacc
     1321 atcacggcta tatcccgcga atgcaggcgg tcatgcaggc ctatggtcat cccaaagagc
     1381 gacttcaagt aatactcgtg cagttggtca agcttctgcg agccggcgaa gaagtgaaga
     1441 tgtcgaagcg gacgggcgag ttcattacga tgcgcgaggt gatcgatgaa gtcggcgcgg
     1501 atgccgccaa gttctacttc ctcatgcggg attcaaagac tcatctcgag tttgacctcg
     1561 agctggccaa acagcgctcg gcggacaatc ccgtgtacta cgtgcaatat gcacacgccc
     1621 ggattgcgag cctctggcgc gtggccgctt cacgtggcat cgcctgccca ctaccaagcc
     1681 agacggacct ctcgttgttg tccgatccag acgaactcgc gctgatccga aaactggccg
     1741 cctatccgtc ggtgctcgaa gccgccgcga tcggctacga gccgcaccgg atgacttatt
     1801 atttgcagca gctcgccgcg ttgctgcaca cgttttacaa caagcaccgc attttgccga
     1861 cggctgccga tcccgaagtg ttcgaacaat ccccatccgg gatgacggcc gacgaggggg
     1921 caaagcggga aacgctgagc cccgaacgga ccgccgcgcg gttagccttg atgcggggcg
     1981 tgcagcaagt catcaagaac ggtttgggag tcctcggcat ttcggccccg gaccagatgt
     2041 aggctaccaa agacaacgat cgt
//
LOCUS       DASUOM010000618         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_810661,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000618 DASUOM010000000
VERSION     DASUOM010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810661"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFY27_12855"
     CDS             complement(<1..247)
                     /locus_tag="VFY27_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766448.1"
                     /translation="MPEITAIHFALFTLLFTIGALLGWIMRGDRSARERIAVNAGWQE
                     RVEAQENEQLRLAEQNTNLMQAVRQYQAAQKDSAKRAK"
     gene            518..1171
                     /locus_tag="VFY27_12860"
     CDS             518..1171
                     /locus_tag="VFY27_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5766449.1"
                     /translation="MIKLPETKTFLFDGPAGRLQALLDMPRKVEAGAAAVVCHPHPEH
                     GGTLENKVVHTLARAFVGKGFAVLRFNFRGVGESEGAFDEGRGETADALAAVGEMKER
                     YRGKPLWLAGFSFGASIAIRAAAEVRADGLVSVAPAVSRVAGAGGPQPGCPWLVIQGD
                     EDDLVPAGDTIEWINSLEPGPELEIFPETGHFFHGKLVRLREAVETFIDENQKKTGR"
     gene            complement(1208..1546)
                     /locus_tag="VFY27_12865"
     CDS             complement(1208..1546)
                     /locus_tag="VFY27_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5766450.1"
                     /translation="MAANDKTGTNSKKAPTKSEIYAQIVEDTGLSKKDVAAVFDSLSS
                     QIKKSLGGRNGSGLFTVPGLLKMRVVKKPATKARQGVNPFTGQEMTFKAKPASKTVKV
                     AALKGLKDMV"
     gene            complement(1709..2185)
                     /locus_tag="VFY27_12870"
     CDS             complement(1709..2185)
                     /locus_tag="VFY27_12870"
                     /inference="COORDINATES: protein motif:HMM:NF025822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HEX5766451.1"
                     /translation="MKPISATIAIAFLPFLAACGGPNEAATVPRAEPAEATSIDIGDH
                     QVHFNAQLTGQLPPEIARTYNIVRSNNRAMLNVSIIDEETRASVPANVTVKTVNLTGQ
                     LKTVNMRRIDEQDAIYYIGETPVANRETLIFDISVMTEGASTPSEVRFKREFFTDQ"
     gene            complement(2391..2939)
                     /locus_tag="VFY27_12875"
     CDS             complement(2391..2939)
                     /locus_tag="VFY27_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5766452.1"
                     /translation="MTQALYFLVKTLAQLYLLLLLLRFWLPWLRADFRNPIAQGILRF
                     TSPLVVPLRRFLPAIGRLDTATILVAFIIQFVVVLVLLAIVRRSVPTPVVAVVSLIEL
                     AILSLNLFFFATLIRIILSWVAPGNYNPITALLTTLSEPVLRPFRRIIPSVGGIDISP
                     IFAILLLQAAVIFLQSIKPVPV"
     gene            complement(2936..3781)
                     /gene="proC"
                     /locus_tag="VFY27_12880"
     CDS             complement(2936..3781)
                     /gene="proC"
                     /locus_tag="VFY27_12880"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5766453.1"
                     /translation="MTRQTVGFIGGGNMARAIAGGLIRSGFGAGNILISTPRPEQQAL
                     LQREFPGAMITGDNCKVAGTAGNLLLAVKPQILRPVCVELKAAVQKQKPLVISIAAGP
                     TVADIDSWLGGNLEIVRVMPNQPALVDQGISALYANPRTDDLGRILAEKVMTAVGKVV
                     WVDDESLMDAVTAVSGTGPAYIYLLIDMMIASAIELGIPPDVARTLVLETAKGAASLA
                     VAEKEPMMSLIDRVRSPGGTTTAAFARLEAADVRAIFASAITAARDRAQELAEEARST
                     RDGGA"
BASE COUNT          728 a         1111 c         1196 g          817 t
ORIGIN      
        1 ccttggcccg tttcgccgag tctttctgtg ccgcctggta ctgtctgacg gcctgcatca
       61 ggttcgtgtt ttgctcggcc aaccggagtt gttcgttctc ctgcgcctcg acccgttcct
      121 gccagcccgc attgacggcg attctttcac gggcggagcg gtcgcctcgc ataatccatc
      181 caagcagcgc cccaatggtg aagagcagcg taaacaacgc gaagtggatg gctgtgattt
      241 ccggcatttc cctgacccct aggctccggt cgcggccgga accattctca taatgccacg
      301 ggcacccgct ccattttgcc aatcacgtca ctaatttacg gcggtttatc cgtgcagcat
      361 ggctgtgtga ctctacagca acctggccta tttctcatac actgccgttg tcgagtaggc
      421 aggcagcttg cgctggtccc cgtaagggca ttagattcga tgccaaagcc gatgcaggtt
      481 atctactgac gccgagctct cccggacgca tcacccgttg atcaaactgc cggaaacgaa
      541 aacatttctc ttcgacggcc ctgccggcag gcttcaggct ctgctcgata tgccgaggaa
      601 agttgaggca ggcgcggcgg cggtcgtgtg ccatccgcat cccgagcatg gcggaacact
      661 cgagaataaa gtcgtgcata cgcttgccag ggccttcgtg gggaaaggtt tcgcagtact
      721 gcgcttcaac ttccgcggcg tcggcgagag cgagggagca ttcgacgagg gccggggcga
      781 aacggcggat gcgctggccg ccgtgggcga gatgaaggaa cgctaccgtg gcaaacccct
      841 gtggcttgcg gggttttcgt tcggagcctc cattgccatt cgtgcggcgg ctgaggttcg
      901 tgcggacgga ctggtcagcg tggcgccggc ggtctcgcgc gtagccggcg cgggaggtcc
      961 acagccgggt tgtccctggc tggtcatcca gggcgacgag gatgatctgg tcccggcggg
     1021 ggatacgatc gagtggatca acagcctcga gccggggcct gaactggaga ttttcccgga
     1081 aaccggccat ttttttcacg gcaagctggt gcgtttgcgc gaggccgtgg agacgttcat
     1141 cgacgaaaac caaaaaaaaa ccgggcgcta agcccggttc tttgtataca ttcacccttg
     1201 cctcgcgtca gaccatatcc ttcaggcctt ttaaagcggc aaccttgacg gtcttcgatg
     1261 cgggcttggc tttgaacgtc atctcctggc cggtaaaggg gtttacgccc tgccgtgcct
     1321 tcgttgcagg ctttttgacc acacgcattt tcagaagacc gggaacggtg aacaggccgc
     1381 tgccgttccg gccgccgaga ctctttttga tttgtgaaga aagcgaatca aagactgcag
     1441 cgacgtcttt cttggaaagg ccggtgtctt cgacgatctg tgcataaatc tcggatttag
     1501 tcggcgcttt cttgctgttc gtgccggtct tgtcatttgc tgccataaaa aagagtctcc
     1561 taaaattgct ggcaaaacga aattgggtgt tcggaaagcg aggattgctc gcgaaaatgc
     1621 tgcgcgacga taacacaaga aaagcgcgcg cgtaaggcgc acaggcattt attaggggtt
     1681 atttgctggt ttttacggcc taggctcgtt actggtcggt aaagaactct cttttgaatc
     1741 gcacttcgga cggggtgctc gccccttctg tcatgacgct gatatcgaag atgagcgtct
     1801 ctcgatttgc aaccggcgtt tcgccaatat agtaaatcgc atcctgttca tcgattcgcc
     1861 tcatgttgac cgtcttcagt tggccggtga ggttgacggt cttgacggtg acgttcgccg
     1921 gcaccgaggc tcgtgtttcc tcatcgatta tcgagacgtt gagcatcgcg cgattgttgc
     1981 tgcgaacgat gttataggtt cttgcgatct cgggcggcaa ctgacccgtg agttgggcat
     2041 tgaagtgtac ctggtggtct ccgatgtcga tcgaggtggc ttcggctggt tcggcccgcg
     2101 gcaccgttgc cgcttcattg ggcccgccgc aggcggcaag gaacggcaga aaggctatcg
     2161 ctatcgttgc gcttatcggt ttcatgttca tcccccttca ggatccctgc ccggacccgc
     2221 ttgaagcgtc cgactatagc ggattgccgc cggttgtcct atggcctccc ctgatgcccg
     2281 gcgactgcgt tcgcatactc ggggagcgac tgccgcgagc cagtatccgg gcattctcac
     2341 gagcaattcc ccgaccggcc tcactcctgt cgctccagcg cgtttgccgg tcagaccggt
     2401 acgggcttga tcgactgaag gaatatgacg gcggcctgca acagcaggat ggcaaagatc
     2461 ggcgagatat cgatgccacc gacgctcggg atgatgcgcc ggaagggcct gaggaccggt
     2521 tccgacaggg tcgtcaggag cgccgtgatc gggttgtagt tcccgggggc cacccagctg
     2581 agaatgatgc gaatcagggt ggcgaagaag aacaggttca ggctcaggat tgcaagctcg
     2641 atcagggaaa cgacggcgac gaccggagtg gggaccgagc ggcgcacgat ggcgagcagg
     2701 acgagcacga cgacgaactg gatgatgaag gcgacgagga tggtggccgt gtccagccgg
     2761 ccgatcgccg gcaggaaacg gcgcaagggc accaccagcg gagaggtaaa gcggaggatg
     2821 ccctgtgcga tcgggttacg aaagtccgcg cggagccacg gcagccagaa gcgcagcaac
     2881 agcagcagca ggtaaagctg cgcaagcgtc ttaacgagga aatatagtgc ctgagtcatg
     2941 cgcctccgtc ccgggtcgac ctggcttctt ctgccagttc ctgcgcccga tcgcgtgcgg
     3001 ccgtgatcgc cgacgcaaag atagcacgaa catctgcggc ttcgaggcgt gcgaacgcag
     3061 cagtggtcgt gccgccgggc gagcggacac ggtcgatcag cgacatcatc ggttcctttt
     3121 ccgccaccgc cagtgacgcg gcaccttttg cggtttcgag taccagcgtt cgcgcaacat
     3181 ccggagggat accgagctct atggcggagg cgatcatcat gtcgatcaac aggtagatgt
     3241 aggcgggccc ggtgccggac actgccgtga ctgcgtccat gagcgactcg tcgtcgaccc
     3301 agacgacctt gccgaccgca gtcatcacct tctccgccag tatcctgccg aggtcatcgg
     3361 tccgggggtt tgcgtaaagc gcggaaattc cctggtcgac gagagccggc tggttcggca
     3421 tcacgcgtac gatctcgagg ttgccgccca gccatgaatc gatgtcggct acggtgggcc
     3481 ccgcggcgat cgaaatcacg agaggttttt gcttctgtac cgccgccttc agttccacgc
     3541 atacgggtcg cagtatctgc ggcttgacgg cgagcaacag gttgcctgct gtgccggcaa
     3601 ccttgcaatt gtcgccggtg atcatggcgc ccggaaattc ccgttgcagc agggcttgct
     3661 gttcaggacg cggtgtcgag atcaatatgt tgccggcgcc gaagccactc cggatgaggc
     3721 cgcctgctat ggcccgcgcc atgttgccgc cgccgatgaa tcccacagtc tgacgcgtca
     3781 tcacgctcct ttcatcgttc actcatgtca gtcgctccgg cgcgggccga agagggcggt
     3841 gccaagcctg ac
//
LOCUS       DASUOM010000619         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_16701,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000619 DASUOM010000000
VERSION     DASUOM010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16701"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFY27_12885"
     CDS             complement(<1..280)
                     /locus_tag="VFY27_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX5766454.1"
                     /translation="MHIRSWQAVLIITAISGAAVVAQVAPGTRFTLATLSLSSGVTAF
                     SLMGAAALLGSRWQFIESLFGGLDRVYLTHKWLGVWALAFASFHLAFKA"
     gene            422..1030
                     /locus_tag="VFY27_12890"
     CDS             422..1030
                     /locus_tag="VFY27_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5766455.1"
                     /translation="MKRSQLEEDKALARRVADGSEAAFEEFFREYFPRLYRFALGRVK
                     GNESAAEEIVQRTMCLVMRKMPGYRGEALLFTWLCQICRNEIASVFREQRIDPQQEVP
                     LEDNPAVQAALASLSVDVGPEGSRSDDEIARFVRATLEYLPARYACALEMKYIRGCSV
                     DEIGEELNISSKAAESVLSRARAAFKEGFRSLWDFEPDFLLE"
     gene            1060..2049
                     /locus_tag="VFY27_12895"
     CDS             1060..2049
                     /locus_tag="VFY27_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5766456.1"
                     /translation="MVNERTTKDAAAGSDPPREDSIAKLMRLAGPRPAVPPDAYERVH
                     AAVKREWRSAVAAQRTRRWAIPAAIAASVVVALVLGGRMLPIDRAPIATVAMTAGADL
                     QVHMRPGDKVYAGDSITTGDNGLSLAFDNGMSLRLAAHTSATLEAIDEVTVTTGMVYA
                     DTGSRTHADSMITVHTDVGSATDYGTQFAVGYDSGAMSVAVREGSVGVDAPRGPFTAE
                     AGERLTLNDGGEARYTKLASHDSSWQWATALAPAFDIDRRPVMDFLRWVARETGKELV
                     FTTDSVRLAAMSTRLSGSVAGLTPVEAIEAVQPTISRFEFHVDEQRVTVSLAQ"
     gene            2363..4891
                     /locus_tag="VFY27_12900"
     CDS             2363..4891
                     /locus_tag="VFY27_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5766457.1"
                     /translation="MSFLARLGVIAALLIACHGVSGAEQSWQGAQLEDYIDELRSRGL
                     RIIYSTDLVHEDYRVQVEPGTDDPEAALREVLAPYGLTLTTGPAGTLLITRAPAELAN
                     VNVRVVDTASGMPVPGARLALDGRQTGVTDEAGRFVMGDVATGQHELRASASGYEGEA
                     TAVFTASGADEVTVRVALDPGPAPLPEIIVTSSHYDIRYQQAGSHTFLDRELTTKLPN
                     LGDEALRTIARLPGATSGGLSSRHHVRGGLQNEQLFLLDGLRLYEPYHLKDFHSITTI
                     VPPGAIAGINFYSAGYQARYGDRMSGVVDIELREPPEKPATELGLTFFHASALSRGAF
                     GGDGRGDWLVSARRANLDLIADVVNPEYGAPRYQDYLAHVGWNIGGHYLSGNALFSND
                     KISLAQPDGSERASARYKNDVLWLKAESEWSERLESRTILSATDIGNRRSGVTEKPGV
                     LEGFVEDGRDFRSLALKQDWLYSISDRWLLSAGVELKRLEADYRYDGALTIAPPFDSI
                     LDNQPSQTRAIDTSPRGAQYAVYTEVRWRPVNSLAIDLGLRWDQQTYTTAMDDEQMSP
                     RVNLLWRLNDRTDLRLAFGQYYQAQEINELQVSDNVVDFHPAQRARHLVASLTHVFPA
                     GTELRLETYQKKYYGLMPRFENVFDPLILIPELQIDRVRIDADRATAEGLEITLSGDG
                     EQDLSWWAGYAWSRSVDELDGLRVPRSWDQRHSVSAGISRDWSHWSISAAALIHSGWP
                     KTVLLADTVPQDGTTELSLYVEPRNSRRHEAFRSLDVRVSRRFDLPRGELSAFLEVTN
                     LLNHENPCCTEYGIELDENGNQLLVSDEGTWLPIIPSLGVLWSF"
     gene            complement(4990..>6009)
                     /locus_tag="VFY27_12905"
     CDS             complement(4990..>6009)
                     /locus_tag="VFY27_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX5766458.1"
                     /translation="VAAIALLPMLGIGGMQLLKAETTGPAKSDKLRPRIKHTAQGLWR
                     LYLFLTIGCALAYWFAGMSAFDAIAHSFSTVSTGGFSIYDASLAHYASPEIEAVAVVF
                     MILGALNFALHFRAWNESSLRPYFINPEARAFVMTIICLTGLVTLVLSTQADIALAEA
                     FRYAIFEIVSVITSTGYGIVDFSVWPALLPVLLIFISFIGGCAGSTAGGMKVIRFLVM
                     SRQAVIEVTRLIHPRLVRPLKVGDSIVSDTVVRAVWAFFTIYAFVFVVMMLGLMWLGL
                     DQVTAFAAIATCLNNLGPGLGEVAANFATVSDPVKLICAFACILGRLEVLTIFVLLTP
                     GYWRR"
BASE COUNT         1270 a         1753 c         1894 g         1092 t
ORIGIN      
        1 ctgccttgaa tgccaggtgg aaggaagcga atgcaagcgc ccagacgccg agccacttgt
       61 gcgtcaggta aaccctgtca agcccgccga agagcgattc gataaactgc catcggctgc
      121 cgagcaaagc agcggcaccc atcagcgaga acgccgtcac gccggaggac aggctgaggg
      181 tggcaagcgt aaaccgcgtc ccgggagcaa cctgggcgac cacggccgcg ccgctgatgg
      241 ctgtgatgat aagaaccgcc tgccagcttc tgatgtgcat gcgcgaaact tccttccttt
      301 gggtttagtc tattgcatcc ggcgcggccg tgagtggcca gctggtattt ctaaaggatg
      361 agcgaaggat ttaccgcgga tcgtcgtcta acggcgacgt aaataaggac cggccggcac
      421 ggtgaaacgc tcccaactcg aggaggacaa ggcgctcgcg cgcagggtcg cggacggcag
      481 tgaggctgcg ttcgaggaat tcttccgcga atattttcca cggctgtacc ggtttgcgct
      541 ggggcgggtt aaaggcaatg agtctgccgc cgaggagatc gtccagcgga caatgtgcct
      601 cgtgatgcgg aagatgccgg gataccgtgg agaggcgctc ctcttcacct ggttatgcca
      661 gatctgccgg aacgaaatag cctcggtgtt ccgggaacaa cgcatcgatc cgcagcagga
      721 ggtgccgctc gaggacaatc cggcggtaca ggcggcgctc gcatcgctct ctgtcgacgt
      781 ggggccggaa ggttcgcgga gcgacgacga aattgcccgt ttcgtgcgcg caacgctcga
      841 atatttgccg gcgcgctacg catgcgcgct ggagatgaaa tacatccgcg gctgttccgt
      901 ggacgagatt ggcgaggagc tgaatataag cagcaaggcg gcagagtcgg tgctgagccg
      961 cgcccgggcg gcattcaagg aaggattccg gtcattgtgg gatttcgaac cggacttcct
     1021 gctcgagtag cggcgagttc ggatcaggag tgaatcacga tggttaacga acgtacgaca
     1081 aaggatgctg cggcgggcag cgatccgccg cgcgaggatt ccattgcgaa gctgatgcgc
     1141 ctggccgggc cgcggccagc cgtgccgccg gatgcttacg agcgtgtgca cgctgccgta
     1201 aagcgggaat ggcgcagcgc tgtcgcagcc cagcggacgc ggcgctgggc gattcccgcg
     1261 gctatcgccg cgagtgtagt cgttgcgttg gtgctcggtg gacgaatgct gccgatcgac
     1321 cgcgcaccca tcgcgacggt cgccatgacc gccggtgcag atctgcaggt tcacatgcgt
     1381 ccgggtgaca aagtttatgc cggcgattcg ataacgacag gtgacaacgg tctttcgctc
     1441 gcgttcgata acggaatgtc gctgcgtttg gcggcacaca cctccgccac cctggaggca
     1501 attgacgaag tcactgtcac gacggggatg gtctacgcgg acacaggctc ccgcactcac
     1561 gcggatagta tgatcactgt tcacactgac gtaggctcgg cgacggatta cggtacccag
     1621 ttcgccgttg gctatgacag cggcgccatg agcgtcgccg ttcgcgaggg cagtgtcggg
     1681 gttgatgcgc cacgcggacc tttcactgcc gaagccggtg agaggctgac gctcaatgac
     1741 ggcggggagg ctcggtacac gaagctcgcc tcgcacgaca gctcctggca gtgggcaaca
     1801 gcactcgcgc cagctttcga tatcgaccgg cgaccggtca tggatttcct gcggtgggtg
     1861 gcgcgcgaaa ccggtaagga actggttttc acgacagaca gcgtgcgcct cgccgcaatg
     1921 tcgacccggc tcagcggatc tgtggcaggc ttgacgcctg ttgaggccat tgaggcagtg
     1981 caacccacca tctcccggtt cgagttccac gtcgatgaac agcgagtgac tgtcagcctg
     2041 gcgcagtagg tgaaatcgct cgagagatca acctcgctct gctcgtatta ccgttagccc
     2101 ggatatctca ccgattcgcc ggaatctcgg gcagccaatt gttcgcataa cgcatttccg
     2161 atcgacgagt agggatggac tcgttagagc gaagcaggat gcccgagccg agcggaatac
     2221 ccgagtgcat ttccgacgag tccaaggatg gactcgttag agcgaagcag gatgcccgag
     2281 ccgagtgagg aaaatgcgtt gtgcggacaa ggggcaagcg agaaaccgag gatcggtgag
     2341 atatccgagc taaaattggc ggatgtcgtt cctggcccgc ctcggcgtga tcgccgcact
     2401 ccttatcgcg tgccatggcg tgtccggcgc cgagcagagc tggcagggtg cgcagctcga
     2461 ggattacatc gacgagctgc gaagccgcgg gttgcgaata atctacagca ccgatctcgt
     2521 ccatgaagat taccgggtgc aggtcgagcc aggcaccgac gatccggaag ccgccctgcg
     2581 cgaagttctt gcgccatacg gcctgaccct gacgaccggt cccgcaggca ccctgctgat
     2641 cacgcgggca ccagcggagt tggctaacgt caacgtacgc gtggtcgaca cagcgagcgg
     2701 catgcccgtc cccggcgccc ggcttgccct cgatgggcgg caaaccggag tcacggatga
     2761 ggcgggccgg tttgtgatgg gagacgtcgc caccgggcaa catgaactga gggcttccgc
     2821 aagcggatac gaaggagagg cgacggcggt attcacggcc agtggcgcag atgaggtcac
     2881 tgtgcgcgta gcgctcgatc ccgggccggc accgcttccc gagatcatcg ttacctccag
     2941 ccattacgac attcgctacc agcaagccgg ttcgcacacc ttcctcgacc gcgagctgac
     3001 gacgaagctg ccgaatcttg gagatgaagc gctgcgcacc atcgcgcgcc tgccgggggc
     3061 cacgagtggc gggttgtcgt cgcgccatca cgtccgtgga ggactgcaga acgaacagct
     3121 tttcctgctc gatggcctgc gcctgtacga gccttatcac ctgaaagatt ttcattcgat
     3181 caccacgatc gttccgccgg gagcgatcgc gggaataaac ttctactcgg ccggttacca
     3241 ggctcgctac ggcgatcgca tgagtggcgt cgtcgatatc gagctgcggg aaccgccgga
     3301 aaaacccgcg acggaactgg gcctcacgtt cttccacgcc tcggcgctat cccgcggcgc
     3361 tttcggagga gacggccgcg gcgactggct ggtttcagcg cggcgcgcca atctcgacct
     3421 tatcgcggac gttgtaaacc cggagtatgg agcgcctcgc taccaggatt acctcgcaca
     3481 tgtcggttgg aatataggcg gccattatct gtcgggtaac gcgctgttct cgaacgacaa
     3541 gatttcattg gcgcagcctg acggcagcga gcgcgcgagt gccaggtaca agaatgacgt
     3601 gctatggctc aaggccgaat cggaatggag cgagcgcctc gagagcagga cgatcctctc
     3661 tgcaacggat atcgggaacc ggcgctccgg cgtgaccgaa aagccgggtg ttctcgaggg
     3721 cttcgtcgag gacgggcggg actttcggtc gctggcgtta aagcaggatt ggctctacag
     3781 catttccgat cgctggctgc tgagcgccgg tgtcgaactc aagcggctgg aagcggacta
     3841 tcgctacgac ggcgcgttga ccatcgcccc gcccttcgac tccatactcg acaaccagcc
     3901 gtcccaaacg cgcgcgatcg acacgtcacc tcgtggagcg cagtacgccg tttacaccga
     3961 agttcggtgg cgcccggtga acagtctggc catcgacctc gggcttcggt gggaccagca
     4021 gacctacacg acggcaatgg acgacgagca gatgagtccg cgcgtgaact tgctctggcg
     4081 attgaatgac cgcaccgatc tgcgtctcgc gttcggccag tactaccagg cgcaggagat
     4141 caacgagctg caggtgagtg acaacgtcgt cgattttcac ccggcgcagc gtgcgcgaca
     4201 tcttgttgca agcctgacgc acgtattccc ggccggcacc gaactgcgac tcgagacgta
     4261 tcaaaagaag tattacggcc tgatgccccg ttttgaaaac gtcttcgatc ccctgatcct
     4321 gatcccggaa ctccagatcg atcgcgtgcg catagacgcg gaccgggcga cggcggaagg
     4381 cctcgagatc acgctgagtg gcgacggcga gcaggacctt tcgtggtggg caggatatgc
     4441 gtggtcgcgg agcgtggatg aactggatgg cctcagggtt ccaaggagtt gggaccagcg
     4501 gcacagcgtc agcgcaggca tcagccgtga ctggagccat tggagtatca gtgcggctgc
     4561 gctcatccac agtggctggc cgaaaaccgt cctgcttgcc gatacggttc cccaggacgg
     4621 aacgacggag ctttccttat acgttgagcc ccgcaacagc cgccggcacg aggccttccg
     4681 gtctctggat gttcgtgtca gccgccgctt cgacttgccg cgcggagagc tgagcgcctt
     4741 tctcgaggtg acgaacctgc tgaaccacga aaacccctgc tgcactgaat acggcatcga
     4801 actggacgag aacggaaatc agctgctcgt cagcgacgag ggaacgtggc tcccgatcat
     4861 tccgtcgctt ggggtgttgt ggagtttctg atcagcttgt cctgagttgc acggtttctt
     4921 tcggcaaagc cgccgtcgag cggacaaggg cagcaggcca tcacctgcac ggctcgcgga
     4981 tccgaaatgt taacgtctcc aataaccggg cgttagcaac acaaagatgg tgagcacttc
     5041 caggcggccc agaatgcatg caaacgcaca aatgagcttg accggatcgc taacggtggc
     5101 aaagttcgct gcgacctcgc caagaccggg gccgaggttg ttgagacaag tagcaatcgc
     5161 ggcgaatgcc gttacctggt caagcccgag ccacatcagg cccagcatca taaccacaaa
     5221 aacgaacgca tagatggtga agaaggccca caccgccctg acaaccgtat cgctcacgat
     5281 actgtcaccc accttgagag gacgtaccag gcgtggatga atgagtcggg tcacctcgat
     5341 aacagcctgg cgggacatca cgagaaaccg gattactttc atcccgcccg cggtggatcc
     5401 ggcgcatccg ccgatgaaac tgatgaagat aagcaatacc ggcaacagcg ccggccacac
     5461 agaaaagtcg acgatgccgt agccggttga ggtgataacg gagacgatct caaatatggc
     5521 ataccggaac gcttctgcta atgcgatgtc agcttgcgtc gagagaacca gggtcaccag
     5581 accagtcagg caaatgatgg tcatcacgaa tgcccgggct tcgggattga taaagtaagg
     5641 tctcaatgag gactcgttcc aggcacgaaa atgcaatgcg aaattgagcg cgccgagaat
     5701 catgaaaaca acagcaaccg cctcaatctc cggacttgca taatgtgcga ggctggcatc
     5761 gtaaatcgag aagccgcctg tggagactgt actgaagctg tgcgcgatgg catcgaacgc
     5821 cgacatgccg gcgaaccagt acgcaagtgc gcatcctatc gtcaggaaca ggtacagtcg
     5881 ccacagtccc tgcgcagtgt gctttatgcg gggacgaagt ttgtcgctct ttgccgggcc
     5941 tgttgtctcg gccttcaata gttgcattcc gccgataccc agcatcggta acaacgcgat
     6001 tgctgccac
//
LOCUS       DASUOM010000620         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1469346,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000620 DASUOM010000000
VERSION     DASUOM010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469346"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFY27_12910"
     CDS             <1..307
                     /locus_tag="VFY27_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5766459.1"
                     /translation="NFFSRATAILATIFFASSLTLAYMSSQRVKGPSSLIERAPIVQP
                     EEDADVTTPDEGANPDLPPLEAEDAPDAGTGDTAEAELPALEPAGTEGGEDDTEGNP"
     gene            323..407
                     /locus_tag="VFY27_12915"
     tRNA            323..407
                     /locus_tag="VFY27_12915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Leu,seq:gag)
     gene            595..951
                     /gene="ndhC"
                     /locus_tag="VFY27_12920"
     CDS             595..951
                     /gene="ndhC"
                     /locus_tag="VFY27_12920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5766460.1"
                     /translation="MLQNYLPVLIFLGVAGGIGLLLLALGFLFGRGRKDDEKLSPYEC
                     GFEAFEDSRMKFDVRYYLVAILFIIFDLEIAFLFPWAVSLDAIGGFGLVAMGIFLGIL
                     VVGFIYEWKKGALEWD"
     gene            1008..1505
                     /locus_tag="VFY27_12925"
     CDS             1008..1505
                     /locus_tag="VFY27_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5766461.1"
                     /translation="MASENASLQKKGFVVSSVDNVINWARTGSLWPLTFGLACCAVEM
                     MHAGASRYDLDRFGIIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPRWVVSMG
                     SCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPSAEALVYGLIQLQKKISRTSTIAR
                     TAITR"
     gene            1517..2212
                     /locus_tag="VFY27_12930"
     CDS             1517..2212
                     /locus_tag="VFY27_12930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5766462.1"
                     /translation="MTDFYETLAARIEARFDAQVERIPSRCGELSYEVRKENLTEVAK
                     ALRDEFAFEQLMDLCGIDYLTYGIDEWTTESATARGFSRAVERESVVMDESDQFDPRR
                     FAVVYHLLSLKNNARLRIRVYTGGDNPPIVVSVVDVWNSANWFEREAFDLYGILFDGH
                     PDLRRILTDYGFIGYPFRKDFPLIGTVEVLYDAEKGRVTYQPVSIEPRTLVPKVIRDD
                     NRYRPELKERPHG"
     gene            2205..>2872
                     /locus_tag="VFY27_12935"
     CDS             2205..>2872
                     /locus_tag="VFY27_12935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5766463.1"
                     /translation="MAEIQSFTMNFGPQHPAAHGVLRLVLEMEGEVVQKADPHIGLLH
                     RATEKLAESKPFSQSIGYMDRLDYVSMMCNEHGYVLAIEKLLGITPPVRAQYIRVMFD
                     EITRILNHLMWLGAHGLDIGAMTVFLYTFREREALMDVYEAVSGTRMHATYYRPGGVY
                     RDLPDSMPRYEPSKWRKQKEVDRLNEARQGTMLDFIEAFVTTFPKNIEQYESLLTDNR
                     IWKQR"
BASE COUNT          612 a          826 c          855 g          579 t
ORIGIN      
        1 gaatttcttt tcgcgagcaa cggcgatact agccaccata ttctttgcca gtagtctcac
       61 gctcgcctac atgagcagcc agcgcgtcaa aggcccctcg agcctcatcg agcgggctcc
      121 catcgtccag cctgaggaag atgctgacgt tacgacgccc gacgaaggtg caaatcccga
      181 tcttccgcca ctggaggcag aagatgcgcc ggacgccggc actggtgata cggccgaagc
      241 ggaactgccg gcactggaac ctgccgggac cgagggcgga gaagacgata cggaagggaa
      301 cccctagaac accagaaaca tagccgatgt ggtggaattg gtagacacgc tgtcttgagg
      361 gggcagtggc gaaagctgtg ccggttcgag tccggccatc ggcaccaccg acaagaacaa
      421 atcagaaatc acaaaaggcc gatcattccg tgatcggcct tttgtctatt gcaggctcaa
      481 agacgcgccc ggggcagggc attacacgga gtctggcaga ctgctacaat tgcgcgctaa
      541 gccgcgctcg ccacgagcgc acacccgact gctggtaaaa agtatctttt tcaaatgctg
      601 cagaattatt tacctgtcct gattttcctg ggggtcgctg gcggcattgg acttctgctg
      661 ctcgcgctgg gtttcctttt tggccgcggc cgaaaagacg acgagaagtt gtcgccttac
      721 gagtgcgggt tcgaagcatt tgaagattcc cgtatgaagt tcgatgtgcg ctattacctg
      781 gtggccatcc tgttcatcat cttcgacctg gagatcgcat ttctttttcc ctgggcggtg
      841 tcgctggacg ccatcggggg atttggcctc gtcgccatgg ggatcttcct cgggatactg
      901 gtggtcggct tcatttacga gtggaagaaa ggagcgttgg agtgggattg agcggaactg
      961 cacaagcgga agcacggaac gataccgcgc cgtccgcgcc gtccgcggtg gccagcgaga
     1021 acgcgagcct gcagaaaaaa ggctttgtcg tctcgagcgt cgacaatgtc atcaactggg
     1081 ctcgcacggg ctccctttgg ccgctgactt tcggcctcgc ctgctgcgcg gtggagatga
     1141 tgcacgcggg tgcttcgcga tacgacctcg accgtttcgg catcatcttc cgccccagtc
     1201 cgcgccagtc ggacgtcatg atcgtggccg gtactctcgt gaacaagatg gctccggcgc
     1261 tgcgcaaggt gtacgaccag atgcccgaac cgcgctgggt cgtctcaatg ggctcctgcg
     1321 cgaacggagg cggctactat cactattcct attcggtagt acgtggttgc gaccgcatag
     1381 taccggttga cgtatacgtt ccaggctgtc caccgtcggc ggaagcattg gtttacgggt
     1441 tgatccagct gcagaagaag atcagccgga cgagcaccat tgcccgcaca gcaataaccc
     1501 gctaagcaac aacttgatga ctgattttta cgagacactg gccgctcgga ttgaggcgcg
     1561 cttcgacgcg caggtagaga gaattccgtc gcgttgcgga gagctttcgt atgaggtgcg
     1621 gaaggaaaac ctgactgaag ttgccaaggc gctgcgtgac gagttcgcct tcgaacagtt
     1681 gatggatctc tgcggtatcg attacctgac ctacggcata gacgaatgga cgacggagtc
     1741 ggctactgcc agaggcttca gtcgcgccgt cgaacgcgag tccgtcgtca tggacgaatc
     1801 cgaccagttc gaccccaggc gctttgccgt ggtctaccac cttctgtcac tcaagaacaa
     1861 tgcgcggctg cgaatccgtg tctataccgg cggcgataac ccaccgatcg ttgtttccgt
     1921 cgtcgacgta tggaactccg ccaactggtt cgagcgtgaa gcgtttgacc tttacggcat
     1981 ccttttcgat ggacaccccg acctgcgacg gatacttacc gactatggct tcatcggtta
     2041 tccgttccgc aaggacttcc cgctcatcgg cacggtcgag gtgctctacg atgccgagaa
     2101 gggccgggtg acctaccagc ccgtgagcat cgagccaagg acactcgtgc cgaaagtcat
     2161 ccgggacgat aaccgctatc gtccggagct gaaggaaaga cctcatggct gaaatccaga
     2221 gcttcaccat gaatttcggt ccgcagcatc cggcagctca cggcgtcctg cgcctcgtgc
     2281 tggaaatgga aggcgaggtc gtgcagaagg cggatcctca tatcggcctg ctgcaccggg
     2341 caacggagaa gcttgcggag tcgaaaccgt tcagccagag catcggctac atggaccggc
     2401 tcgactacgt gtcgatgatg tgcaacgagc atggctacgt cctcgccata gagaaactgc
     2461 tcgggatcac gccgccggta cgagcgcagt acatacgcgt catgttcgac gagatcacgc
     2521 gcatcctcaa ccatctcatg tggctaggcg cgcatggcct cgacatcggc gccatgacag
     2581 tgttcctcta caccttccgg gagcgagagg cgctcatgga cgtgtacgaa gcggtctccg
     2641 gaacccggat gcacgcaacc tattaccggc ctggcggggt ctaccgtgac ctgccggatt
     2701 cgatgcccag gtacgagccg tccaagtggc gcaagcagaa ggaagtggac cggctgaacg
     2761 aggcccggca aggaaccatg ctcgatttta tcgaggcctt cgtaacgacg ttcccgaaga
     2821 atatcgagca gtacgaatcg ctgctgaccg acaacaggat ctggaagcag cg
//
LOCUS       DASUOM010000621         6368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_572024,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000621 DASUOM010000000
VERSION     DASUOM010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572024"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFY27_12940"
     CDS             <1..363
                     /locus_tag="VFY27_12940"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX5766464.1"
                     /translation="TLRRKSLTATAGAPDIVDQEVAPGIENLQVQFGVDVDRDNTIDR
                     YVNPGAGILDPAHADYIPSARVMTARVWLVVRSIDREMGIVDNRSYEPGDVNLGVPND
                     DFRRIQVSKTILLRNART"
     gene            403..876
                     /locus_tag="VFY27_12945"
     CDS             403..876
                     /locus_tag="VFY27_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEX5766465.1"
                     /translation="MNAAKKQNGAALVVSLILLVVVTVLAVSGMNTATTELAMARNDQ
                     NYENAFQAAETGLETALARGSFDTTSTSIPTQTIVVPERLFDTVQVLIEFDDTTIVPD
                     KAFSLGAGSGIAAYHFRAIATAESARAGGGTETDRDASAVHTQGFYIVGPGTPTL"
     gene            922..1260
                     /locus_tag="VFY27_12950"
     CDS             922..1260
                     /locus_tag="VFY27_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766466.1"
                     /translation="MNIRMMLAAVVIGLSFQALANFAVVSEAYEVPLADIRLPANANG
                     TLAFKACETCDYATIRVTTTTQYEANNSVLDLEAFRKELERIRNPREVTATVLHHLES
                     NTIQAIRIKF"
     gene            1294..4638
                     /locus_tag="VFY27_12955"
     CDS             1294..4638
                     /locus_tag="VFY27_12955"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX5766467.1"
                     /translation="MKAQTGNPAWTLAGFLLALTSGAPALADDTELLLQIPDIDSKPN
                     ILFILDSSGSMDEVVRTQEPYDAGRTYEGGTCDATRVYWTDVDLPPDCSATTQSVAKS
                     AFVCEAAAGQLTGIGSYADTMVQHRETDIAGVSRWQDLEPGNHVDLVECRADSGEHGM
                     GTAGELWPSNTAGAPFTSDPDRELAWGSAPAAITYTVYDCNYLNWKSSPATVDLPKID
                     ILKSVSKAVLNSVTDVNVGIMRFQGDHGGTVIKAISDLDSDRASILQTIDDIDANGNT
                     PLAETLYESALYWRGQSAHFGERWDPSRTDPAALVSLDPEAYKQPQMQVCSKNFNVLL
                     SDGQPTQDVDTPTLLGNLPDFPAAGCDGSGAGHCLDDVAHYLANVDIHADDGDQLVST
                     HTIAFDLDDAAAIKLLQDTAQESGGSFFFADDVETLALALLNIIAEITERSLSFTAPS
                     VSVNTFNRTQNLNDLYLTVFSSRTKVHWPGNLKKFTVENGQIVDASGSLAVNPATGFF
                     YDTAKSIWTDGTADGNDVMLGGAAHELPTPASRNLYTNNDGTNNLVAGANALHPTNVA
                     AFTGADFGLTGGTGEPTIDEMIRWMRGEDLRDEDIDGSTADTRNAIGDPLHSQPASIV
                     YGGSPESPDVVVFVGTNDGYLHAIDGSTGQELWSFVPKELLGDMNRLYFDPTSNFKHY
                     GIDGNIVPVMKDTDMDGDIEPGDGDFVYILFGLRRGGSSFYALDVTVKNSPQLLWVKQ
                     YPEFGQSWSTPVVTRVNIDHGGSVGERPVVIVGGGYDTVHDTRTHPAAPDAQGAGIHM
                     LDLVTGQRLWHAGHTDSDADLKLASMTRAIPTQIRVIDMNGDGRADRMYAADLGGQLW
                     RFDIINGEDPLNLVNGGVIARLGAEGLGSPSDADTRRFYNAPDAAIFNDPLQGRRFIS
                     ISIGSGYRAHPLDETAADRFYSIRDPDVFTQLTQAEYNAYDIITEADLEDVAGRVRVV
                     IGSEDRGWMFTLPGSQKVISDSITFDDSVFFVGFSPEENTADPCAPSNGRNFLYEVNI
                     VNGDPVVNNLDALADADADAERVTPLAQGGIAPTPAFLFPAPTDPDCEGKDCSPPPIG
                     CVGVECFDPGFANNPVRTLWTQDGVE"
     gene            4635..5081
                     /locus_tag="VFY27_12960"
     CDS             4635..5081
                     /locus_tag="VFY27_12960"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX5766468.1"
                     /translation="MRPLHLCRAAGTSRERGVTLIELLIVVVIVSILAAVAYPNYRQY
                     VQKSKRNEAKAALLQIATNQERFYLNQNTYTNDMTSLGFPADDNVLTGSGTYSVDVTA
                     ADANTFTATATYQLDDEEKAKCETFTITATGAQTSSPLADCWTRTQ"
     gene            complement(5190..5711)
                     /gene="lspA"
                     /locus_tag="VFY27_12965"
     CDS             complement(5190..5711)
                     /gene="lspA"
                     /locus_tag="VFY27_12965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5766469.1"
                     /translation="MPSADPTGRKPFTLYLAVSLLIVALDQLTKWAILTWVPLYDKVA
                     VNSFIYITHQQNRGAAFSFLANASGWQRWFFTVLAIAVSIAIVVWLWRIRREGLVVLS
                     SGLALVLGGAVGNVIDRLRFGHVVDFIQVLIAGWPFPSFNVADSAITVGAALLIIDAL
                     FISGRAKPVEQSS"
     gene            complement(5851..>6368)
                     /locus_tag="VFY27_12970"
     CDS             complement(5851..>6368)
                     /locus_tag="VFY27_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX5766470.1"
                     /translation="HLRRAGRALDFSVTQVPSVVVDGERVSSTAIRQALWNGELNRAS
                     RLLGRHYSMSGTVIRGDNLGRALGFPTANVNLRRRQSPVMGIFAARVRGLGREPLDAV
                     ASVGTRPTFGGTKPLLEVHIFDFDEDIYGAYIQVDFIARLRSEEKFGGAEELVEQMHR
                     DSARARKILAA"
BASE COUNT         1380 a         1929 c         1883 g         1176 t
ORIGIN      
        1 accttgcgac gaaagtcgct gacagcaaca gctggcgcac cggacatcgt cgaccaggaa
       61 gtcgcacccg gaatcgaaaa cctgcaggtc cagttcggag tcgacgtcga ccgggacaac
      121 accatcgacc ggtacgtgaa ccccggagca ggaattctcg atcctgccca cgcggactac
      181 ataccgagcg cgagagttat gaccgccagg gtgtggctgg tggtgcgctc gatagatcgc
      241 gagatgggca tcgtcgacaa ccgttcctac gagccgggcg atgtgaacct tggtgtgccg
      301 aacgacgatt ttcgccggat acaggtatcg aagacgatcc ttttgagaaa tgccaggacc
      361 tgacggcagg cacacgatgc ggatgatacg gttatgaaag aaatgaacgc tgcaaaaaaa
      421 cagaacggtg cggcgcttgt cgtcagcctg attctactgg tcgtggttac tgtactcgcg
      481 gtatccggca tgaacacggc cactaccgaa ctggcaatgg ctcgcaacga ccagaactac
      541 gagaacgctt tccaggctgc cgaaacgggg ctcgagacgg cgctggcgcg cggcagcttt
      601 gacacgacga gcaccagtat tccaacgcag acgattgtgg tgccggagcg gctgttcgac
      661 acagtgcagg tgctgatcga attcgacgac accaccattg tgccggataa agctttcagc
      721 ctcggcgccg gcagcggaat tgcggcttac catttccgcg ccatagcgac tgcggaatcg
      781 gcgcgggccg ggggcggtac ggagactgat cgcgacgcat ctgcagtgca cacgcaaggt
      841 ttctacatcg tcgggcccgg gacgccgact ctctgaggca gcccttaaaa atattggaac
      901 ggatgatttg ggacgggtga aatgaacatt cgaatgatgc ttgccgcagt agtgataggc
      961 ctcagcttcc aggcattggc caatttcgct gtcgtatcgg aagcttacga agttccgctc
     1021 gcagacatcc ggcttccggc aaatgcgaac ggtactcttg cgttcaaggc ttgtgaaacc
     1081 tgcgactacg cgaccataag agtcacgacg accacgcaat atgaagcgaa taacagcgtt
     1141 ctggatctgg aggcgtttcg aaaggaactc gagcgcattc ggaacccgcg ggaggtaacg
     1201 gctaccgtcc tgcatcacct cgaatcaaac acgattcaag ctatacgtat caagttctga
     1261 ccgagcgacg ggctgaagga tcaaggaaag aatatgaaag ctcaaactgg gaatcctgcc
     1321 tggacgcttg ccggcttttt gctcgcgctg acctcaggtg cgccggcgct tgccgacgat
     1381 actgaactgc tgctccagat accggacatc gacagcaagc cgaacatcct gttcatcctc
     1441 gattcctcgg gcagcatgga cgaggtcgtc cggacacagg aaccgtacga tgccggccgc
     1501 acatacgagg gcggcacctg cgatgcgact cgcgtgtact ggaccgatgt cgatctgccg
     1561 ccggactgct ctgctaccac gcaatctgtc gccaagagcg ccttcgtctg cgaagcggcc
     1621 gcgggacagc tgacaggcat cggcagttac gcggatacca tggtgcagca tcgcgaaacg
     1681 gacatcgccg gtgtctcccg atggcaggat ctcgagccgg gcaatcatgt cgacctggtg
     1741 gagtgccggg cggactccgg tgaacatgga atgggcacgg cgggcgagct gtggccttcg
     1801 aataccgccg gcgcgccctt taccagcgac ccggaccggg aactcgcgtg gggcagcgcg
     1861 cccgcagcca taacctatac ggtatacgac tgcaactacc tgaactggaa gagttcgcct
     1921 gcaacggtcg acctgccgaa gatcgacatc ctcaaatccg tttccaaggc cgtactgaat
     1981 tcggtcaccg atgtgaatgt cggcatcatg cgtttccagg gcgaccacgg cggcacagtc
     2041 atcaaggcga tcagcgacct ggattccgac cgggcaagta tcctgcaaac gatcgatgat
     2101 atcgatgcaa acggcaatac gccccttgcc gaaaccttgt acgaatccgc actctactgg
     2161 cgtggccaga gtgcccactt cggcgaaaga tgggatcctt cgagaacaga cccggcagca
     2221 ctggtgtcgc tggatcccga agcatacaag cagccgcaga tgcaggtctg ctcgaaaaac
     2281 ttcaacgtcc tgctgtcaga cggccagcca acgcaggatg tcgacacacc aactttgctc
     2341 ggcaacctgc cggattttcc cgctgccggc tgtgacggct ccggtgcagg acattgcctc
     2401 gatgacgtgg cgcactacct ggctaacgtc gatatccatg ccgacgacgg ggaccagctg
     2461 gtatcgaccc ataccatcgc gttcgacctg gacgatgcgg ccgcaatcaa gcttctgcag
     2521 gatacggcgc aggagtccgg cgggtcgttt ttcttcgctg acgacgtaga aaccctggcg
     2581 ctggctttgc tgaacattat tgccgagatc acggaacgct cgctatcgtt cactgctccg
     2641 tcggtgtcgg tcaacacgtt caaccggaca cagaacctca acgacctgta cctgacggtg
     2701 ttctcctcga gaacgaaagt gcattggccg ggcaatttga agaaatttac cgtggaaaac
     2761 ggccagatag ttgatgcctc cggctcactt gccgtgaacc ccgctaccgg attcttctac
     2821 gacacagcga agagtatctg gactgacggt acggctgacg gcaatgacgt catgctgggt
     2881 ggtgcagctc acgagctgcc gactcctgcg tctcgcaatc tttatacgaa caatgacggc
     2941 accaacaacc tcgtcgccgg ggcgaatgcc ttgcatccaa cgaacgtcgc cgcattcacc
     3001 ggggcggatt tcggcctgac cggcggcacc ggcgagccca cgatcgacga gatgattcgc
     3061 tggatgcgcg gcgaggacct gagggacgag gacatagatg gttccaccgc tgacacgcga
     3121 aacgcgatcg gagacccgct gcattcgcag ccggcgtcga tcgtctacgg cggcagcccc
     3181 gagtcgcccg acgtcgtcgt cttcgtggga accaacgacg ggtacctgca tgccatcgac
     3241 ggaagcacgg gacaggaact ctggtccttc gtgcccaagg aattgctggg ggacatgaat
     3301 cggctgtact tcgatcccac ctccaacttc aagcactacg gaatcgacgg taacatcgtg
     3361 ccggtaatga aagatacgga catggacggc gacatcgaac ccggtgacgg cgacttcgtg
     3421 tacatcctgt tcggcttgcg gcggggcggc agttctttct acgcgcttga cgtgaccgtc
     3481 aagaattcac cgcaacttct gtgggtgaag cagtacccgg aattcggcca gagctggtcg
     3541 acgccggtgg tcacgcgggt gaatatcgac cacggcggct cggtcggtga acggccggta
     3601 gttatcgtcg gaggcggcta cgatacggtg cacgacactc gtactcatcc cgcggccccg
     3661 gacgcgcagg gagccgggat tcacatgctg gacctcgtga ccggacagcg tctctggcac
     3721 gcaggacaca ccgattccga cgccgatctg aagctcgcgt ccatgacccg cgccattccg
     3781 acccagatcc gtgtcatcga catgaacggc gatggccgcg cggaccgcat gtacgccgcc
     3841 gacctgggcg gtcaactgtg gcggttcgac atcatcaatg gcgaggatcc gcttaacctc
     3901 gtcaatggcg gagtgatcgc gcgactgggc gccgagggcc tcggttcgcc atcggatgcc
     3961 gacacacgcc gcttctacaa tgcgccggac gccgcaatct tcaatgaccc gctgcaaggc
     4021 cgacggttca tttccatcag catcggcagc ggctatcggg cgcatccgct ggacgagacg
     4081 gcagcggaca ggttctactc cattcgcgac ccggacgtat tcacgcaact cacccaggct
     4141 gaatacaatg cctacgacat catcacggaa gcagatctcg aagatgtcgc cggacgcgtc
     4201 agggtcgtca tcggttccga agaccgcggc tggatgttta ccttgcccgg cagccagaag
     4261 gtaatctccg actccatcac gttcgacgac tccgtgttct tcgtcggatt ttcgccggaa
     4321 gagaatacgg cagatccctg tgcaccgagc aacggccgca acttcctgta cgaggtaaac
     4381 atcgtgaatg gtgatccggt ggtcaacaac ctcgacgcac tggctgacgc cgatgcggac
     4441 gccgagcgcg tgacccctct tgcacaaggg ggcatcgcgc ccacgcccgc gttcctgttc
     4501 ccggctccaa ccgacccgga ctgcgaaggc aaagactgct caccgccacc gataggctgc
     4561 gtgggtgtcg agtgcttcga tcccggattt gccaataacc cggtgcgaac gctgtggaca
     4621 caggatggcg tggaatgagg ccgctccacc tttgtcgcgc ggccggcacc tcgcgggagc
     4681 gcggggttac gctgatcgag ctcctgatcg tcgtcgttat cgtcagcatc ctggcggcgg
     4741 tggcctatcc gaactaccgg cagtacgtgc agaagtcgaa gcgcaacgaa gccaaggccg
     4801 cgctgttaca gatagctacg aaccaggaac ggttttatct gaaccagaac acctatacga
     4861 acgacatgac gagcctcggc ttcccggcgg acgataatgt gctgacaggc tccggaacgt
     4921 attcggtgga cgtgacggcc gcggacgcaa acacgtttac ggccactgcg acgtatcagc
     4981 tggatgatga ggaaaaagcg aagtgcgaaa ccttcaccat cacggcaacc ggcgcacaaa
     5041 cctcctcgcc actggccgac tgctggacca ggacccaata agctctgtag gagcccgcca
     5101 tgcgggcgag tttttgatgc ttcagtgaac attcgcccgc acggcgggct cctacagtcg
     5161 gctattcctt tctcgccgca agcggaagcc tagctgctct gctccaccgg ttttgctctt
     5221 cccgagataa acagcgcgtc gatgatcagc agcgcggcgc ccacggtgat ggctgaatcc
     5281 gcgacgttga acgacggaaa cggccaaccg gcgatcagca cctggataaa gtccaccacg
     5341 tggccgaagc gcagccggtc gataacgttg ccgaccgcac cgcccagtac cagggcaaga
     5401 ccagacgaca gcaccaccag cccctcccgc cgaatacgcc acaaccacac cacgatcgca
     5461 atgcttacgg cgattgcaag aacggtgaag aaccaccttt gccagccgga ggcattggcg
     5521 agaaagctga aagcggcccc ccggttctgc tgatgtgtga tgtagatgaa cgagttcacg
     5581 gcgaccttgt cgtagagcgg cacccacgta aggattgccc atttcgtgag ctggtcgagc
     5641 gctacgatga gcagggatac agcgagatat agcgtgaacg gtttacggcc ggtggggtcg
     5701 gcgctgggca ggactgcagt agtgtcgttg tcagtcaaaa gttgcttcct tggttgtcgt
     5761 gttgattcat ttatcgatcc gaagatgcct gcggtctggt gtcgcccgcg gagcgggctc
     5821 ctgcagcgct ttttacgaaa ccaggcggca ctaggccgcg agtatctttc ttgcgcgggc
     5881 gctgtcacgg tgcatctgtt caaccagttc ttcagccccg ccaaacttct cttcgctcct
     5941 tagccgggct atgaaatcga cctgaatata ggcgccataa atatcctcgt caaaatcgaa
     6001 gatgtgcacc tcgagcagcg gtttggtgcc gccaaaggta ggtcgggtgc ctacgctcgc
     6061 aacagcgtcc agcggctcgc gtcccagtcc gcggacgcgc gcggcaaata tacccatgac
     6121 cggactctga cgtcgccgca ggttcacgtt cgcagtagga aaacccagcg cgcggccgag
     6181 gttgtcgccc cggatgacgg tgcccgacat gctgtaatgc cgcccgagca ggcgcgatgc
     6241 gcggttgagt tcgccattcc atagcgcttg ccggatggcg gtgctgctga cgcgctcgcc
     6301 gtcgacaacg acgcttggca cctgcgtcac cgaaaaatcg agcgcgcggc ctgcccgccg
     6361 caaatgct
//
LOCUS       DASUOM010000622         6693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_206554,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000622 DASUOM010000000
VERSION     DASUOM010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206554"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFY27_12975"
     CDS             complement(<1..303)
                     /locus_tag="VFY27_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5766471.1"
                     /translation="MKRMLINATQAEELRMAMVDGQRLYDLNIELPSGERKKANIYKG
                     KITRIEPSLEAAFVNYGADRHGFLPLKEISSEYFINDPAPGAKPEIKHVLKEGQDIV"
     gene            727..1713
                     /locus_tag="VFY27_12980"
     CDS             727..1713
                     /locus_tag="VFY27_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5766472.1"
                     /translation="MQEKSEKTAPGERSGVRKVEVDAEGAGQRVDNFLRRELPGVPKS
                     RLYRLLRRGEVRINGGRVRADHRLEAGDEVRIPPARVRQPGADPSAAQAARLAERVLF
                     EDKRLLVIDKPSGTAVHGGSGVSHGVIELLRFARPDLKDLSLVHRLDRETSGCLVLSK
                     RRSALRALHEQFREGSVEKNYLALVAGDWQSGEVVVEAPLYVQNRKGGERHVIVSDEG
                     KAAATRLSLSRRFGDFSLLQCQPLSGRTHQIRVHAAHRGHPLVIDERYGEEETNEAAR
                     AAGLTRLFLHAQSIAFPDDSGNDLHFTAPLADDLEAFLTKLETRPGAGGRRW"
     gene            complement(1757..2350)
                     /locus_tag="VFY27_12985"
     CDS             complement(1757..2350)
                     /locus_tag="VFY27_12985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5766473.1"
                     /translation="MQDPSAPPLILASSSPHRRGLLDRILTEYQTVSPDVDESREGDE
                     GPLEQAVRLARTKAEAVASTHTRQLVIGADQLAVLGRRVLGKPGDHQHAIDQLLASSG
                     KSLRFLTAVCVLDPVARRRHEHVDETLVRFRRFDRRLAENYLRHDKPYDCAGSFRIEG
                     AAFVLFESVTTDDPTALIGLPMIWLGGKLQELGYLLS"
     gene            2554..3144
                     /locus_tag="VFY27_12990"
     CDS             2554..3144
                     /locus_tag="VFY27_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5766474.1"
                     /translation="MRNPLLDRSSPGELADLGQVVETKTNLGDFSRLSGVVEADLASL
                     PEAEFPREWRQAPVQIRLRFSWIDAGRRLPALEGRVTGSIPVVCQRCLGPMELALDEE
                     LKLLLVAPGESAGAKGAADDFEIWELDEATIRPLDILEEALIMAMPLSALHERGQGCD
                     AAVDAEPPVREETNRPFAGLRSQISGRDGEETGSSG"
     gene            3238..3429
                     /gene="rpmF"
                     /locus_tag="VFY27_12995"
     CDS             3238..3429
                     /gene="rpmF"
                     /locus_tag="VFY27_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5766475.1"
                     /translation="MAVQKSRKTPARRGMRRSHDKLGKPTLSVDPTTGETHLRHRVTP
                     DGFYRGNQVIAQVEEDDEE"
     gene            3512..4522
                     /gene="plsX"
                     /locus_tag="VFY27_13000"
     CDS             3512..4522
                     /gene="plsX"
                     /locus_tag="VFY27_13000"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5766476.1"
                     /translation="MGTKSVIALDAMSGDLGAEVVVRAAVAALEQHPNLSLVLVGNEQ
                     ELQRLVTNLSGVAQRLTICHATEVVGMSESAADALRKKKDSSMRVAIDLVKEGKADAC
                     VSAGNTGALMATAKFVLKMLPGIERPAIIAELPAIGGTVHMLDLGANTASDAELLFQF
                     ALMGSIVTSDIRRIEHPRVALLNIGVEASKGHEAIKDAATLLAASGLNYVGFIEGNEI
                     FSGKADVVVTDGFTGNIALKTMEGTVGLASHYLRRAFTRSLLTKVQAFFAAPVLKSLA
                     VEMDSRNYNGASLVGLNGIVIKSHGSADAYAFQHAIETALVEVKNQVPMQIGNLLQQE
                     AA"
     gene            4519..5487
                     /locus_tag="VFY27_13005"
     CDS             4519..5487
                     /locus_tag="VFY27_13005"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5766477.1"
                     /translation="MIYSRIAGTGRFLPERILTNADLEKMVDTTDEWIRTRTGVERRH
                     IASEDQTTSDLSVAAAKIAMDAAGVAADEIDLIVVGTTSPDLIFPNIGTLVQHRLGVH
                     GCPAFSIEAACTGFIYALTTADKFVRAGEAKCALVIGAEIISKLVDWEDRSTCVLFGD
                     GAGAVIIKPSEEPGIISCHLGADGQYKELLYYPVGASKDLHKAGVGDSKIIMSGNEVF
                     KVAVKTLGNVAEQALAANNISKDEIDWLIPHQANLRIIQATARRLGMPMDKVILTVQD
                     HGNTSAASVPMALDVGVRDGRVQRGQLILMEAFGGGFTWGSVLMRY"
     gene            complement(5502..6047)
                     /locus_tag="VFY27_13010"
     CDS             complement(5502..6047)
                     /locus_tag="VFY27_13010"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX5766478.1"
                     /translation="MGVSISVELTDRDLKFFRYAVKKSRDAVRDADEQEIIEAIQSVV
                     DVIKVESPLPDFISRRLPELDAMIAMLKDTEWKLPKTDRERLLATFVYFGDPEDIIPD
                     DIPGIGFIDDVILIELLIRDLRHVREAYDDFCEFRDSYGTKYGRDPDPAMRRKRLESR
                     RKQLHERMKRRQRREKTTALW"
     gene            6256..>6693
                     /locus_tag="VFY27_13015"
     CDS             6256..>6693
                     /locus_tag="VFY27_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5766479.1"
                     /translation="MKPVLFIGNKNYSSWSLRAWFLLKEAGIEFDEVRIPLDTPEFSE
                     RIGSISPTRKVPVLKLDGDVVWESLAIAETVAEHWPERKLWPHDPHLRAHARSLSAEM
                     HSGFMALREAMPMNCRAMGRKVTLTDAVTGDINRISAIWGECRP"
BASE COUNT         1341 a         1951 c         2143 g         1258 t
ORIGIN      
        1 gacgatgtcc tggccttcct tcaggacatg cttgatctcc ggtttcgcgc ccggagcagg
       61 gtcattgatg aagtattcgc tcgagatttc cttgagcggc aggaatccgt gccggtcggc
      121 gccgtaattg acgaaggccg cttcgagact cggctcgatg cgtgtgattt tgcctttgta
      181 gatgttagct ttcttgcgtt cgcctgacgg cagttcgatg ttgaggtcgt acaggcgctg
      241 gccatcgacc atcgccatgc gcaactcttc tgcctgagtt gcgtttataa gcattctttt
      301 catgttgccc tgctttgatg tagtggggca gcaagcggat acggcgggaa cgcgaagggg
      361 ccaggcaggg tgcctcaaca tggagagcct ggcgcaacgc gccagcaatg aggttgctaa
      421 gcttgaacca ggcgaggtgg caagccatcc cgcacagcaa aaaaataatc attgttaccc
      481 tgcggcgcag ccgctatggc gtttgccggc ctttcttgct tgctgtcaaa cggagtccgg
      541 tctctaaagg tacgaggcct ggaatgaatt tgattacgcg cgaccgctgc cggggcagac
      601 gagccaaggc tttgaggccg gcgtagtcaa caagtctgtg ggacggggaa aaggactccc
      661 cggctttcac agcgcgctaa tataacacac agttccaagc tggcaatgcc gtacaagtcc
      721 ctgtctatgc aagagaagtc ggaaaaaacc gctcccggcg aacgctcggg agtgcggaaa
      781 gtcgaggtcg atgccgaggg ggcagggcag cgcgtggaca atttcctgcg cagggagctg
      841 ccgggggtac cgaagagccg tctctaccgg ctgctgcgcc ggggagaggt gcgcatcaac
      901 ggcggccggg tgcgggcaga ccatcgcctc gaggcaggcg acgaagtgcg gattccgccc
      961 gcccgggtcc gtcagcctgg cgccgatcca tcggccgcgc aggcagcgcg cctggccgag
     1021 cgtgtgctgt tcgaggacaa gcgcctgctg gtgatcgaca agccgagcgg caccgccgta
     1081 cacggcggca gcggcgtcag tcacggcgtc atcgagctgc tgcgtttcgc ccggccggac
     1141 ctgaaggacc tgtcgctggt gcaccggctg gaccgggaga catccggctg cctggttctg
     1201 tcgaagcgac gctcggcact gcgggccctg catgagcagt ttcgtgaagg ctcggtagag
     1261 aagaactacc tggcgctggt ggccggggac tggcaatccg gggaggttgt agtcgaggcg
     1321 cccctctacg tgcagaaccg caagggcggt gagcgccacg tcatcgtcag tgacgagggc
     1381 aaagccgcgg ctacgcgcct cagcctgtca cgccgcttcg gggacttcag cctcctgcag
     1441 tgccagccgc tctcgggacg gacccaccag atccgggtgc atgccgcgca ccggggccat
     1501 ccgctcgtga tcgacgagcg ctacggcgag gaagagacca acgaggccgc cagggcggca
     1561 ggtctcacgc ggctgttcct gcacgcacag tcgatcgcct ttcccgatga cagcggcaac
     1621 gacctgcatt tcaccgcgcc gcttgctgac gacctggagg cgttcctgac gaaactggaa
     1681 accaggccgg gagcaggcgg ccgccgctgg taaaatgaga aaaagggaac cgactcgttt
     1741 accgcccctt taaccgtcag gaaaggaggt accccagctc ctgcagtttc ccgcccagcc
     1801 agatcatcgg caggccgatg agagccgtcg gatcatccgt ggttacggac tcgaacagga
     1861 cgaaagcggc gccttctatt ctgaaacttc cggcgcaatc gtaaggcttg tcatgacgca
     1921 ggtagttttc cgcgagtcgc ctatcgaagc gcctgaagcg caccagcgtt tcgtcgacgt
     1981 gctcgtggcg gcgccgggca accggatcga ggacgcaaac cgcggtgaga aacctgagcg
     2041 atttcccgga cgaagcgagc aactggtcga tggcgtgctg gtggtcgccg ggcttgccca
     2101 gcacgcgtcg ccccagcacc gccagttgat cggctccgat cacgagctgc cgggtgtgtg
     2161 tgctggcgac tgcttccgcc tttgtgcggg ccaggcgcac cgcctgttca agcggtccct
     2221 cgtcgccttc acgtgattcg tccacgtccg gagagacagt ctggtactcg gtgaggatac
     2281 ggtcgagcag gccgcgtctg tggggcgacg acgatgccag gatgaggggc ggggccgagg
     2341 gatcctgcac agctgaacct tttctagaca cgagtggcgg gtgagaagca gatctgttgt
     2401 tgacctgatt gagcggcggt attgccgcag gaagaataaa cctgcccact tattttgctg
     2461 tgtaagttta acctgaaggc ccggaccgct tgcgaacgcg ctggcgtaag cgcctttgac
     2521 acctgggaat caggtcctta tgatgcgccc gccatgcgca atccgctgct agatcgaagc
     2581 tcgcccgggg aactggcgga ccttggccaa gtagttgaaa ccaaaacgaa cctgggagac
     2641 ttctcccggc tttcaggggt cgtggaagcg gatctcgcgt cactgccgga ggcggagttc
     2701 ccccgagaat ggcggcaggc acccgtacag attagactcc gcttcagctg gatagatgcc
     2761 ggtcggcggc tgccggccct cgagggccgg gtgaccgggt ccattccggt ggtttgccag
     2821 cgatgccttg gtccgatgga gctggctctc gatgaagagc tgaagttgct gctggttgcg
     2881 ccgggggaga gcgcgggggc gaagggcgca gcagacgact ttgagatctg ggagttggac
     2941 gaggcgacga tacggcctct cgacatcctt gaagaagcac tgatcatggc tatgccgctg
     3001 tccgcgctgc atgagcgcgg gcagggctgt gacgcagcgg tggacgcgga gccgcccgtc
     3061 agagaggaaa cgaaccggcc gtttgccggt ctacggtcgc agatttccgg ccgcgacggc
     3121 gaagaaaccg gcagttccgg ataacccccg cgcacgcagc cgccggaaag agctgctgac
     3181 aaggaatgac tgaatcaatg gcggcgcgag aatcgccgcc gttcaatgga gagtgaaatg
     3241 gctgtacaaa agagtcgcaa gacacccgca cggcgtggca tgcgtcgatc gcacgacaaa
     3301 ctgggtaagc ccacgctgtc ggtggatccg accacgggag aaacgcacct gcgtcatcgc
     3361 gtgaccccgg atggttttta tcgcggcaac caggtgattg cccaggtcga agaagacgac
     3421 gaagaataac ctggcatccg gccggtccga ataacaaccc tcgcatggcg tgccatcgcg
     3481 tgtttaacaa gcggtccggc cagacgactt cgtgggaaca aaatcagtaa ttgcgcttga
     3541 tgcgatgagc ggcgacctcg gcgccgaggt tgtcgtgcgc gcggccgtcg cggcgctgga
     3601 acaacatccc aacctgagcc tggttctcgt cggcaacgaa caggaactgc agcgacttgt
     3661 aacgaacctg tcgggggtcg cgcaacggct aacgatatgt cacgcaacgg aagtggtcgg
     3721 catgtcggaa tccgcggcgg atgcgctgag gaaaaagaaa gactcctcga tgcgtgtggc
     3781 gatcgacctc gtcaaggaag gcaaggccga cgcctgcgtc agcgccggta acaccggtgc
     3841 gctgatggct accgcgaagt tcgtgctgaa aatgctgccc ggcatcgagc ggcccgcgat
     3901 cattgccgaa ttgccggcca tcggcggaac cgttcacatg ctcgacctgg gcgcgaacac
     3961 agccagcgac gccgagctcc tgttccagtt tgccttgatg ggttccatcg taaccagcga
     4021 catacggcgt attgagcacc cacgcgtggc gctcctcaac atcggtgtcg aagcatccaa
     4081 gggacacgaa gcgatcaagg atgcggctac gctgcttgcc gcgagcgggc tcaattacgt
     4141 agggttcatc gagggcaacg agattttttc cggcaaggcc gacgtcgtcg tgactgacgg
     4201 tttcaccggt aacattgccc tgaagacgat ggagggcact gtcgggctcg catcccacta
     4261 tcttcgccgt gccttcaccc gctcattgct tacgaaggtg caggcgtttt tcgcggctcc
     4321 cgtgttgaag agcctggcgg tcgaaatgga ttcgcggaac tataacggtg cgtctctcgt
     4381 gggacttaat ggtatcgtca taaaaagtca tggcagtgcc gacgcgtacg cattccagca
     4441 tgcaatcgag acggccctgg tcgaggtcaa gaaccaggta ccgatgcaga tcggtaacct
     4501 cttacaacag gaagccgcat gatctattcg cgcatcgcag ggaccggccg atttctgcct
     4561 gagcgcatcc tgacgaacgc ggacctcgaa aaaatggtcg acaccaccga cgagtggata
     4621 agaacccgaa cgggtgtcga gcggcggcat attgcttccg aggaccagac tacatcggat
     4681 ctctctgtcg cagccgcgaa gatcgcgatg gatgccgccg gcgtcgcggc ggacgagatc
     4741 gacctgatcg tcgtgggaac caccagcccc gacctgatct ttccgaacat cggaaccctg
     4801 gtgcagcacc gcctcggcgt gcatggctgc ccggcgttca gtatcgaggc tgcatgcacc
     4861 ggctttatct acgcactgac aactgccgac aagttcgtaa gggccggtga agcgaaatgc
     4921 gccctggtga tcggcgccga gatcatctcg aagctggtgg actgggaaga tcgcagcact
     4981 tgcgtgctgt tcggcgatgg tgcgggcgcc gtgataatca aaccctcgga agagccgggc
     5041 attatttcct gccaccttgg cgcagacggc cagtacaagg agttgctcta ttacccggtc
     5101 ggtgcgtcga aagacctgca caaggcgggt gtcggcgact cgaaaatcat catgagcggc
     5161 aacgaagtgt tcaaggttgc ggtaaagacc cttggcaacg ttgccgagca ggcgctagcg
     5221 gcgaataaca tcagcaagga cgagatcgac tggctcattc cgcaccaggc gaaccttcgt
     5281 attatccagg cgactgccag gcggcttgga atgccgatgg ataaagtcat cctgaccgtg
     5341 caggatcacg gcaacacgtc tgcggcatcg gtgcccatgg cgctcgacgt gggggtgcgc
     5401 gacggccggg tgcagcgcgg ccagctgatc ctgatggaag cgttcggcgg cggctttacc
     5461 tggggctcgg tccttatgcg ctattgacgc agcttccgtc gttaccagag cgcggtcgtt
     5521 ttctcgcggc gttgacggcg tttcattcgt tcatgcagtt gcttgcggcg agactcgagg
     5581 cgcttgcggc gcattgccgg atccgggtcc cggccgtatt tcgtgccgta gctgtcgcgg
     5641 aattcgcaaa agtcgtcata agcctcgcgg acgtggcgca ggtcgcgaat cagcagctcg
     5701 atcaggatca cgtcgtcgat gaagccgatc cccgggatat cgtcggggat gatgtcttcc
     5761 ggatcaccga agtagacgaa cgtagccagc agccgctcgc gatccgtctt cggcagcttc
     5821 cattctgtat ccttcagcat cgcgatcatc gcatcgagct ccggaagccg ccgcgaaatg
     5881 aaatcgggca gggggctttc caccttgatg acatcgacga ccgactggat cgcctcgatg
     5941 atctcctgct catccgcatc gcgtacggcg tcgcgcgatt tcttgacggc atagcggaaa
     6001 aacttcaagt ctcggtccgt aagttctacc gaaatactga cgcccatttc gatgcaccgc
     6061 tgccttcgag gcggacaggc taccggcggc agctataccg gtcaattcct gtcttgctgc
     6121 gccgcaccag ggagcctctg cacaagtctg gatggaaaga tcccgtctct gacttgtgca
     6181 caggctccct ggcctgtgcc cggggcccaa ctgtggaata atgttcgtgt cgaggcgaag
     6241 ggcacgtaca gtcgcatgaa accggttctc tttattggca acaagaacta ttcaagctgg
     6301 tcattgcggg cctggtttct gctgaaggaa gcaggaatag agttcgacga ggtacgcatc
     6361 cccctcgaca cgccggagtt ctcggagcgc atcggcagca tctcaccgac gcgtaaagtg
     6421 ccagtgctga agctagatgg cgacgtggtc tgggagtcgc tcgcaatcgc cgagacggta
     6481 gcggagcact ggccggagcg gaagctgtgg ccacacgatc ctcacctccg ggcgcacgcc
     6541 cgttccctga gcgccgaaat gcactccggt ttcatggcat tgcgtgaagc aatgcctatg
     6601 aactgccggg caatgggccg taaagtgacg cttaccgatg cggtcacggg cgacatcaac
     6661 cgcatctctg cgatatgggg cgagtgtcgc ccc
//
LOCUS       DASUOM010000623         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1291628,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000623 DASUOM010000000
VERSION     DASUOM010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291628"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFY27_13020"
     CDS             <1..883
                     /locus_tag="VFY27_13020"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5766480.1"
                     /translation="MVTTRQLAYEREDLAEAEFALNSDAGGGDLNVDGKALVYNIQAA
                     EKTYVTWEMTARNAGGHSSRPRPDNAIYDLADAIGKIRAHRFPVRWNPMTLQFFQVTG
                     EQMGGELGDAMVRFAKNPEDTTASDRLAVESSYVGTTRTTCVVTMLRAGHAENALPQS
                     ATATVNCRIFVGVPAAEVLQTLQQVVGNDAIEFKETYAPVESPVSELREDVRAAISEA
                     VQGRYPGVRMIAYMESGGTDGMHFRRAGIPTWAASGIFMNPDEMFAHGLNERVPIKTF
                     YDALDHWTIIIKELTGD"
     gene            complement(1438..2328)
                     /locus_tag="VFY27_13025"
     CDS             complement(1438..2328)
                     /locus_tag="VFY27_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766481.1"
                     /translation="MNASAFVRLPGLWWYVAAWLSIAPAGPAHALSDAAAALRAKYES
                     IHASLGSSPFRQPLLLRAAPGDDSLKGEVFAVINQPFSVTGATLQDKTRWCDVLILHL
                     NVKQCRVRGGNGAGTLALVVGRKFEQPASAGRAVDFEFRVLNAGPDYVRVQMAAEDAP
                     SDARNQVMLEVAPLDDRRSCLHVTYSYVFSMMERMGMRAYLSTLGRDKVGFTTTGTGA
                     DGKPAYVRGVRGMVERNVMRYYLAIVTYVESLAVPLAERQEKRLRDWFAATERHALQL
                     HEMGREEYLAMKRNETRGMR"
     gene            complement(2647..>3421)
                     /locus_tag="VFY27_13030"
     CDS             complement(2647..>3421)
                     /locus_tag="VFY27_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX5766482.1"
                     /translation="ENRFTSEEELIEETRGLVSVTREKVMPLFTKLPAQELIVEPFPG
                     FLRGTGQSSRYEQKPESEGPATYRIATDDWETQTRGRAAIVLVHEGWPGHHLQIATAR
                     DIEGLHPAIRLASSGAYIEGWARYSEALGEEAGIYEAGYGEITRRAWPARGMVMDPGL
                     HLYDWTRERAIAYAVESGNFTPDTAEDLVDRIAVWPGQLTAYDTGGLEIFALRKQAEE
                     QLGDAFDIREFHDRILENGALPLGALREHVETWIAEKQK"
BASE COUNT          683 a         1084 c         1023 g          631 t
ORIGIN      
        1 catggtgacc actcgtcagc tggcctacga gcgcgaggac cttgccgagg ctgaattcgc
       61 attgaactcc gacgcggggg gtggcgacct gaacgtggat ggcaaggcgc tggtctataa
      121 catccaagcg gcggaaaaga cctacgtcac ctgggagatg acagcgcgca acgccggagg
      181 ccacagttca aggccgcgac cagacaacgc gatctatgac cttgctgacg cgatcggcaa
      241 gataagagcc caccgcttcc cggtgcgctg gaaccccatg acacttcagt ttttccaggt
      301 gacgggtgaa caaatgggtg gtgaactcgg cgacgccatg gtccggtttg cgaagaaccc
      361 cgaagataca acggcatccg atcgacttgc cgtggaatcg tcctacgttg gcacaacccg
      421 aacgacatgt gtcgttacga tgttgcgggc aggtcatgcg gaaaatgcgt tgccacaatc
      481 cgccacggcg actgtcaatt gccgcatctt cgtcggcgta cccgccgctg aagtgctcca
      541 gacactgcag caggtcgttg gcaacgatgc gatcgagttc aaggaaacat acgcacccgt
      601 cgaaagcccg gtttcggaac tgcgcgaaga tgttcgcgcc gccatcagcg aagcagtgca
      661 ggggcgctac cccggcgtaa gaatgattgc ctatatggaa tcgggcggga cagacggcat
      721 gcattttcgc cgcgcgggga ttcccacatg ggcggcgagc ggcattttca tgaatccaga
      781 cgaaatgttc gctcatggcc tgaacgaaag agtgcccatc aaaacgtttt acgacgccct
      841 ggatcactgg accataataa tcaaggagct tacgggcgac tgaagtgagc aaaaagaaga
      901 cttgacctca cttgctgcgg cgttccgaaa gctcggcggc tgaaggtaag caagctgcta
      961 gcgatctgcc cggaacaaga tgtaaccaac ggggcacagg cggtgcacgc aaatctcccg
     1021 catgtctcga gatagagaaa cgcgtttgag cgggctattg tgcgatcgtc ggaagctgtc
     1081 cggcgatttc catccgtacc gggctcgcca tccagctttt gttgcaggag ctttaatatc
     1141 gcagggcgat cgtcctgaac catcagcgga gtctgcccag ttgctcggga tcgagggcgg
     1201 cggtacgcat tgcccactgg tttgcaaggt cgtatcgccc aggtccaggt aggcaaacgc
     1261 aagcatcaag gctgaacccc ggaacgcgcg tcccggtagc gcagccgccc agtaattccc
     1321 gaagcccccg tcgtcacctc taacaatgcg gaaaggtacg taccatcctc tcggttcggg
     1381 cgggcgtctt tcaagcagtc aaaaaccgag cgccaacaaa taagcgaccg tggacggtca
     1441 ccgcatgccg cgcgtctcgt tgcgcttcat cgccaggtac tcctcgcgtc ccatctcgtg
     1501 caactgcaac gcatggcgtt cagtagctgc aaaccagtcg cgcaggcgct tttcctggcg
     1561 ctccgcaagc ggcaccgcca gcgactcgac gtaggtgact atcgcgaggt agtagcgcat
     1621 cacattgcgc tcgaccatgc cacgcacgcc gcgcacgtat gccggcttac cgtccgcccc
     1681 ggtgccggtg gtggtgaaac cgaccttgtc gcgtcccagc gtcgacaggt aggcccgcat
     1741 ccccatgcgc tccatcatgc tgaagacgta ggaataggtc acgtgcaggc agctgcgcct
     1801 gtcgtcgagc ggcgccacct cgagcatcac ctggttcctg gcgtcgctcg gggcatcctc
     1861 ggcggccatc tgcacacgca cgtagtcggg ccccgcgttg agcacgcgaa attcaaagtc
     1921 cactgcccgc ccggcactcg ccggctgttc aaacttgcgg ccgactacca acgccagcgt
     1981 cccggcgccg ttgccgcccc gcactcggca ttgcttgacg ttgaggtgca ggatcagcac
     2041 gtcacaccag cgggttttgt cttgcagagt ggcgccggtg acgctgaagg gctggttgat
     2101 caccgcgaag acctcgccct tgagggagtc gtcgccgggt gccgcccgta gcagcaaggg
     2161 ctgccggaac gggctgctgc ccagcgaagc gtgtattgac tcgtacttcg cgcgcaacgc
     2221 ggcagcggcg tcggacaacg cgtgcgccgg cccggcgggc gcgatcgaca accacgcagc
     2281 cacgtaccac cataggccgg gcagccgaac aaaagcgctg gcgttcaacc tgatggctcc
     2341 ttcgtggaca aatgccctgg atactgagat tagcgggccc atgactttag gtaaggtctt
     2401 caatccttca attcacgcaa gacttaccca aggccggccg aacgcgccga gaaggtgaat
     2461 cgaatgatgc tcccgctcac gctgtcctgc gggcccgcta aggccgtttc tgcgccggct
     2521 gcacgacgtg gtcgccgatc accagccccg ccgcagttgt tcggaggtaa gcacaattcc
     2581 gccagccctg acgggaaacg ccggcgaacg gccgggtcga tcggtactct cccgttgtga
     2641 gccgacttac ttctgcttct cggcgatcca ggtctcgaca tgctcgcgca aagcaccgag
     2701 cggcagcgcg ccgttttcga ggatgcggtc gtgaaactcg cggatgtcga aggcatcgcc
     2761 aagttgctcc tcggcttgct tccgcagtgc gaagatttcg agcccacctg tgtcataggc
     2821 cgtaagctgg cccggccaga cggcgatgcg atcgacgaga tcttccgccg tatccggcgt
     2881 gaagtttccg gattcgaccg cataggcgat cgctcgctcg cgggtccagt catagaggtg
     2941 cagtcccgga tccatcacca tgccgcgtgc cggccaggcg cgccgcgtga tttcgccgta
     3001 gcctgcctca tagattcccg cctcttcccc cagcgcttcc gaatagcgcg cccaaccttc
     3061 gatataggcg ccggagcttg caagacggat cgccggatgc aggccctcga tgtcgcgcgc
     3121 cgtcgcgatc tgcagatgat ggccgggcca tccttcatgc accaggacaa tagccgcccg
     3181 accgcgcgtc tgtgtctccc agtcatcggt cgcaatgcga taggtcgcag gcccttcgga
     3241 ttccggtttc tgctcgtaac gcgaggactg gccggtgcct ctcagaaaac ccggaaacgg
     3301 ctcgacgatc agctcctgcg cagggagttt cgtgaagagg ggcattacct tctcacgcgt
     3361 cacggagacg agcccgcgcg tctcctcgat gagttcctct tcgctcgtga aacggttctc
     3421 c
//
LOCUS       DASUOM010000624         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1088752,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000624 DASUOM010000000
VERSION     DASUOM010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088752"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..412
                     /locus_tag="VFY27_13035"
     CDS             203..412
                     /locus_tag="VFY27_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5766483.1"
                     /translation="MSTFTIQHEQEEDGRWIAEVPELPGVLAYGATADEAMAKAEVLA
                     LRVLAERLEHKEAKPQSITIGIAAA"
     gene            409..621
                     /locus_tag="VFY27_13040"
     CDS             409..621
                     /locus_tag="VFY27_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX5766484.1"
                     /translation="MSSWPSSRATRVLAALYRIGWSLKRQSGSHKTLSRQGWPDFVFG
                     FHDRDEIGPRMLSRIAKHTGLKPEDL"
     gene            726..1556
                     /locus_tag="VFY27_13045"
     CDS             726..1556
                     /locus_tag="VFY27_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5766485.1"
                     /translation="MTNAKTIADHWGRGDVYGLIVSALNKMSKPLDSLTVEDLAPADH
                     FHARGFPATVELADRLPLKTGQHIVDIGCGLGGPARYLAKRFQCNVSGVDITEPFVEA
                     ANKFTALLRMEGQVNIEHGDGQHLPYADSAFDGAVTQHVTMNVADRPAFFAEAYRVLK
                     PGAFFALTEHGLGEKGNPHYPVPWSMDGSGAYLVTPSNTSALLQQAGFVDVRIEDTGT
                     KYAAGYKAAIEKAQRGELPPLGMHLLMGETAPQKMRNALRNIEEGRTHPIQVICRKPG
                     "
     gene            1538..2095
                     /locus_tag="VFY27_13050"
     CDS             1538..2095
                     /locus_tag="VFY27_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766486.1"
                     /translation="MSQTRINLLQAAANLLLPPTHTGARLSATLAGMAPVEIGDQHRD
                     LVARLRPIILPWRKLTIAIDGVDNSGKSTLGRFLAWQLGMLLIETDLLLKDKGGDIDH
                     DLVTFTRLLNSRHDLNRPAIVEGVLVLRTLEKVSVRPDFMVYVETNGRDGSEVWQGMF
                     KAYAAKYRPRESANFTFRWSSEHAS"
     gene            2261..2605
                     /locus_tag="VFY27_13055"
     CDS             2261..2605
                     /locus_tag="VFY27_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766487.1"
                     /translation="MLTKAFMPYHAAVLDKSWPVLEPRLQIIILGMLKVAGGGLLGYG
                     LSLLWLLLPLQRGEVWAAWAALTVSLAVVGPILYVVVSLRRIEPSAKTPIVPTLVVLV
                     LVMVATVASLAR"
BASE COUNT          576 a          850 c          872 g          596 t
ORIGIN      
        1 tattgcgaaa tctgtggccg ctgatcgttg ctcacgcata taccgactat gtgtggttca
       61 catgacaatc gaatgcccgt gagtgctttg gcaactaacg gcgcgttggt gtccgacgcg
      121 tacctcgcgc tgcgcgcttc gtcccgcgtg gcaaaacgcg gacgttgggt ggcaaattcg
      181 gacgcagcgt agaatcgcag ctatgtcgac cttcaccatt cagcacgagc aggaagaaga
      241 cggccgttgg atcgcggaag ttcctgaact tcctggtgtt cttgcctacg gcgcgacggc
      301 tgatgaagcc atggcgaagg ctgaagtcct ggctctaaga gtgctcgcgg aacgcctcga
      361 gcacaaggaa gccaagcctc agtccatcac cataggtatc gcagctgcgt gagttcgtgg
      421 ccttcgtcca gggccactcg agtactcgca gctctctatc ggatcggctg gtcgctcaag
      481 cgccagtccg gttctcacaa aactctttcg cggcaaggct ggccggattt cgtcttcggg
      541 tttcacgatc gcgacgagat cggtccgcga atgctttcgc gcatcgccaa gcacactggt
      601 ttgaagccgg aggacttgtg agcgcctcgc tttgccaccc aactataggt ggaacggacc
      661 ggccaaaggc ggccggccgt tcaccacaac gttagccgcc aatctacgac ggtcgaggag
      721 gtgaaatgac gaatgccaaa actattgcgg accattgggg aagaggtgac gtctacggcc
      781 taattgtgtc ggcattgaac aagatgtcga agcccctgga cagcctgacg gttgaagact
      841 tggcgcctgc ggatcatttc cacgcgcgcg gctttcccgc gaccgtcgag ctcgcagatc
      901 ggcttccgct aaagaccggc cagcatatcg tcgatatcgg gtgcggcctg ggcggccctg
      961 cacgctactt ggcaaagcgg tttcaatgca atgtgagtgg cgtcgacatc actgagccat
     1021 tcgtcgaggc ggccaacaaa ttcacggcgc tgctccgcat ggaggggcaa gtgaatattg
     1081 agcacggcga cggtcagcac ctcccgtacg cggactccgc cttcgatggc gccgtcacac
     1141 aacacgtcac catgaacgta gccgatcgcc cggcattctt tgcggaagcc taccgcgtat
     1201 tgaaaccagg cgccttcttt gcgcttaccg agcacggtct aggagagaag ggtaatcccc
     1261 actaccccgt gccctggtcc atggacggaa gcggcgctta cttggtgacg ccgtcgaata
     1321 caagcgcgct tctgcaacag gctggattcg tcgacgtccg gatcgaggac accggcacaa
     1381 agtacgcagc ggggtacaaa gccgcaatcg agaaggcgca gagaggcgag ttacccccac
     1441 tgggaatgca tctactcatg ggtgagacgg caccgcagaa gatgcgcaat gcgcttcgaa
     1501 atatcgagga gggtcgcacc catcccatcc aagtgatatg tcgcaaaccc ggataaatct
     1561 tctgcaggcc gcggctaacc tgctgctgcc gccgacgcac accggcgcgc ggctgagcgc
     1621 cacgttagct ggcatggcgc ccgtggagat tggtgaccag caccgagatc ttgtagcgcg
     1681 cttaaggcca attatccttc cgtggcggaa actcactatc gcgatcgacg gagtcgacaa
     1741 ctcggggaag tcaacgctcg gtcgatttct ggcgtggcag ctcggaatgc tattgataga
     1801 aaccgacctc ctgcttaagg acaaaggcgg cgacatcgat catgacctgg tcacattcac
     1861 gcgcctgctc aactcgcgtc atgatttgaa ccgtcccgct attgtggaag gtgtgctcgt
     1921 attgcgaacg ttggagaagg tcagcgtcag gccggatttc atggtctacg ttgaaaccaa
     1981 tggccgtgac ggaagcgaag tttggcaggg gatgttcaaa gcatatgccg cgaagtatcg
     2041 acctcgtgag agcgcgaact tcacgttccg ctggagttcg gaacatgcca gctaacagcc
     2101 ggttgttgcg cgacgcttcc gaatcggcgc tcctgcgtcg cgcctccttc cgcgcgccaa
     2161 aacgggaacg ttaggcgcca gggtacagct tatgcaaatc gtagctcttg tatcgctcgt
     2221 attggcagct gtgctcgctc tcgcctttgg agcacgctac gtgctgacca aggccttcat
     2281 gccctatcac gcggccgttc tggacaagtc gtggcccgtg ctggagccgc gcctccaaat
     2341 catcatcctc ggcatgctca aggtggcagg tggtggcctc ctgggctacg gcttgtcgct
     2401 gctgtggctg ttgctgccgc tacagagagg cgaagtctgg gcggcctggg ctgcgcttac
     2461 tgtttcgttg gcagttgtcg ggccgattct ctatgtcgtc gtgtcgctgc gtcgtatcga
     2521 gccgagcgcc aagacgccca tcgttccgac gctcgtcgtc ttagtgctgg tgatggtggc
     2581 cacggttgcc tctctcgccc gctgatgggg tgcgctttgg cgcctaacag cacgccgcac
     2641 acggacgcgc gcacgagcgc cgtgcttcgc atagctcacc gggcgcgcgc cggtgggcgt
     2701 ggacgctagg cccctaaccg acataggagg tccgcagatg gcgcaattac gagaggcttt
     2761 gcctcaactc agcggcgatt tcttccttac cgacggcgga atcgagacaa cgcttatctt
     2821 ccttgagggt caggagctgc cggatttcgc ggcgtttcat ttgctaaaga ccaaggatgg
     2881 cgaggctgcc ctac
//
LOCUS       DASUOM010000625         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1000880,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000625 DASUOM010000000
VERSION     DASUOM010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000880"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFY27_13060"
     CDS             <1..528
                     /locus_tag="VFY27_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766488.1"
                     /translation="GIPCIYYGTEQQFEGHGRNDRFIREAMFGGEFGAFESRGRHFFN
                     ETGTVYRELQKILRLRREHLVIRRGRQFLRQISDDGQNFGLPVMISNAIRCVVPWSRI
                     FSDREVLLAVNTDVDVPRTAWVTIDNGLHAPGTQLTCLYSTNAAQIGSIVDIEPRNGK
                     AVQLTVPAAGFVAFA"
     gene            complement(662..1909)
                     /locus_tag="VFY27_13065"
     CDS             complement(662..1909)
                     /locus_tag="VFY27_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX5766489.1"
                     /translation="MKPLRSSGVGFRVGESPVGNLKEEEMVNSNAKQKILCVLYDDPV
                     DGYPPKYARDDIPKLSRYPDGQTLPTPSGIDFMPGELLGCVSGELGLRKFLEERGHSL
                     VVTSDKEGENSALDKELRDADVVISQPFWPAYLTAERIAKAQNLRLAITAGIGSDHVD
                     LQAAIERGITVCEVTYCNSISVSEHAVMQILTLVRDYIPQYGWVIKGGWNIADAASRS
                     YDVEGMNVGVLAAGRIGLAVLKRMKPFDVKLHYHDRHRLPDEVEKELGVTFHKDAESL
                     ARACDVLSIHCPLHPETEHLFDDELIGKMKRGSYIVNTARGKICDRDAIARALESGQL
                     AGYAGDVWYPQPPPKDHPWRSMPHHAMTPHTSGTSLSAQARYAAGTREILECWFDGKP
                     IREEYLIVEGGKLAGAGAHSYSV"
     gene            2688..>2887
                     /locus_tag="VFY27_13070"
     CDS             2688..>2887
                     /locus_tag="VFY27_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766490.1"
                     /translation="MPVVGFVLTAAGWAVMWGVVSFWNPIAFAAMWTGAAILMWCASR
                     SGYPGSRRHLALAMVSIPLWWW"
BASE COUNT          519 a          895 c          870 g          603 t
ORIGIN      
        1 ggtattccgt gcatctacta tggcaccgag cagcagttcg aaggccacgg tcgcaatgac
       61 cgctttatcc gcgaggcaat gttcgggggc gagttcggcg cgttcgaatc ccgaggccgg
      121 cacttcttca acgagaccgg caccgtgtat cgagagcttc agaagatcct gcgtctgcgt
      181 cgcgagcatc tcgtaattcg ccggggacgg cagttcctgc gacagatttc ggacgatggt
      241 cagaacttcg gtttgccggt gatgatcagc aacgcgatcc gatgcgtcgt gccgtggtcg
      301 cgtatcttca gcgaccgcga ggttctgttg gcagtgaata ccgatgtcga cgtacccagg
      361 acagcgtggg tcactatcga caacgggttg cacgcgcccg gcacccagct cacgtgtctc
      421 tactccacga acgccgctca gatcggcagt atcgtggaca tcgagccgcg caacggcaag
      481 gcggtgcagc tgacggtgcc agcagcgggg ttcgtcgcat tcgcgtaggt ccgcgcaggg
      541 cgtcttttac tcgaagacat caccgctgat tgcgcacacc gctagcgtac cgaaagacga
      601 gcgctgcagc atcaacgcgc atcattcatg aacggtcgag atgggcggtg gggcagcgtc
      661 gctacacgct atacgagtgc gcgcctgcgc cggccagttt acccccctct acgatcaggt
      721 actcctccct gatcggtttg ccatcgaacc agcattcgag gatctcccgc gtacctgccg
      781 cgtaacgcgc ctgagccgac agcgacgtgc cagaggtatg gggcgtcatc gcatgatgcg
      841 gcatgcttcg ccacggatga tccttgggag gtggctgtgg ataccagacg tcgccggcgt
      901 aaccagccag ttgaccgctc tccagcgccc tggcgatcgc gtcccgatcg cagatctttc
      961 cgcgcgcggt attcacgata taggagccgc gcttcatctt gccgatcagc tcatcgtcga
     1021 acaggtgctc cgtctcggga tggagcgggc agtgaatgct gagcacgtcg catgccctgg
     1081 ccagtgactc cgcgtctttg tggaacgtca cgccgagttc tttttcgact tcgtcgggca
     1141 gcctgtgcct gtcgtgataa tgcagcttca cgtcgaacgg cttcatacgc ttgagcacag
     1201 ccagcccgat gcgaccggca gccagcacgc cgacgttcat accctccacg tcgtacgacc
     1261 gcgaggcggc atcggcgatg ttccagccgc ctttgatgac ccagccgtat tgcgggatgt
     1321 aatcgcgaac aagggtcagg atttgcatga ccgcgtgttc ggacacgctg atgctgttgc
     1381 agtaggtgac ttcgcatacc gtgattccgc gctcgatggc cgcctgcaga tcgacatggt
     1441 ccgagccgat acccgccgtg atcgccagtc tcaggttctg cgcttttgcg atgcgttcgg
     1501 ctgtcaggta ggcgggccag aacggctggg atatcaccac gtcggcgtcg cgcagctcct
     1561 tatcgagtgc ggagttctcg ccctccttgt cggacgtcac aacgagtgaa tggcctcgtt
     1621 cttcaagaaa cttccgcagg cccagttcgc cggacacgca gccgagcagc tcgccgggca
     1681 taaaatcgat gccgctcggc gtcggcagcg tctgcccgtc aggatacctg gaaagtttcg
     1741 gtatatcgtc gcgcgcgtac ttcggcgggt agccgtcgac cggatcgtcg tacagcacgc
     1801 aaaggatttt ctgcttcgca ttggaattga ccatctcttc ctccttcaag ttaccaaccg
     1861 gggattcgcc gacgcgaaac ccgacgccgc tcgaacggag tggcttcatt ccgtgacggt
     1921 tcgcgtcagg tgaccacgtg cgtgttgccg agcgtggatg aatgtataga gagtaacgtc
     1981 atagcggctg ggcagctcga ccggaacgtg tcggcgatat tcctgctggt gtctgcgtat
     2041 cgcagagggt atcgaccaag gagcgcttcg caccgccacc acgtagctgt cggcgttcca
     2101 gtaagagcct cggcttgacc gggcacccgc acatcgggac ctccgcctcg tccgcaaccc
     2161 cgccaagccg gtcaccgccc ggctgacggt attcgcggta agatcgccgt ggacgaaaaa
     2221 agctcgcgag tttcgacgac ccatcttgcc cgaacgtcgt ttaagtatag cgtatccgcc
     2281 accggagtct ttgcgccact caccgaggat gctgatgtcg tcgagggcat cctcgtatac
     2341 ctcaatgtta ggacccctta ccgacccgat atcacccgtc ggtcccgcgc cgccgtggct
     2401 tcaagccgac gcccttcccc tcagctacca ccctgtgcgg atatcgctga gcgcccggcc
     2461 ttccgccgca gcacaaactg ggcacttgca gcagctgctt cggtgcggcc gaccccgaac
     2521 tccgctataa attacccgcc cactaaatta cggcatacac ttgctcgcat ctgtgagaaa
     2581 gtcgaagaat tcctgcaagc gcgcccgcgt cgcatgcgta tgaaggagcc cacggctctg
     2641 gttgcactgc cgctgtgaac gcggccgcga gacggagtgg gatatttgtg ccggtggtgg
     2701 gcttcgtgtt gaccgctgcc ggttgggccg tcatgtgggg cgtcgtatcg ttctggaatc
     2761 cgatcgcgtt cgccgcgatg tggaccggcg cggcgattct catgtggtgc gcttcgagat
     2821 ccgggtatcc aggttcacgg cggcatctgg cgcttgcgat ggtgtcgatt cctttatggt
     2881 ggtggtt
//
LOCUS       DASUOM010000626         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_202459,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000626 DASUOM010000000
VERSION     DASUOM010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202459"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1461
                     /locus_tag="VFY27_13075"
     CDS             175..1461
                     /locus_tag="VFY27_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5766491.1"
                     /translation="MNRNRTASVLIALQVFAWIVTPAAAETVVVSAARMLDVERGTVT
                     GPVQVVVRDGLIESINPDRLPPDARVLELGDRTLLPGLLDMHTHVTLDYFTGSYWVTA
                     PVFQTAPDWAIMGVNFAGQTLQAGFTTVRDVGAWPGFPDVALMRAIDAGTVPGPDVWP
                     AGHYISITGGHCDITGFAPGIQELGPKQGIADGVDEVLKAVRYQAKHGVRVIKVCATG
                     GVFSFSNNAAVGALQYSLEELEAIVREATKLGLKVAAHAHGTEGINTAVRAGVSSIEH
                     GSILSDESIELMKEHGTYLVPNLYINDLPLPPETPAATVAKSDYLEPLVVQSLQKAYK
                     AGVNMALGTDAGVFPHGQNGREFSALVANGIPELHALKMATVYAADLLGVDDRGVIAP
                     GKRADLVAVPGNPLDDIKVMEDVRFVMKQGMVYRQE"
     gene            1698..2450
                     /locus_tag="VFY27_13080"
     CDS             1698..2450
                     /locus_tag="VFY27_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766492.1"
                     /translation="MQANVDNLIATLSRRKTGLFALLMTALLSALPQQSAADVALSLA
                     HFAAGERALEGRVALPDVEGDLTLLLQCRTFVSKRGSARNTFCLDHDRELESLLATLH
                     DAVNKSRMIPAMVDGKPEAVQILFRVLFVRRGTHASVHVYENWGHDVDTLGFDYRAPQ
                     LWSRKGTSYKCFRHDRPLVAQATIRISETGLPSGQASVEFAEGSAEDAGCEQQLKEEL
                     AASAFIPAEANGKPVEATFTTVIGWVTSSQAN"
     gene            2823..3164
                     /locus_tag="VFY27_13085"
     CDS             2823..3164
                     /locus_tag="VFY27_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5766493.1"
                     /translation="MGAKRRSLTQDERKQIAAKTGSRCHICGGPLEPRWHADHVLAHS
                     GGGRHSVDNYLPAHATCNNYRWDYSSTEFAEILKLGVFFRTAIEKGDAHADKLARSYL
                     AKERQRQRRMR"
     gene            complement(3181..3396)
                     /locus_tag="VFY27_13090"
     CDS             complement(3181..3396)
                     /locus_tag="VFY27_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766494.1"
                     /translation="MVVAQVDWGCWVVSVTRQELLELLDRWYGAGPIYPPDWTIRERG
                     NREDNEIRQFVESLDGSTRYLLTALET"
BASE COUNT          732 a         1087 c         1102 g          691 t
ORIGIN      
        1 caggttcgct cacaacaggt aaccgcagaa tacgaaggct ccggtgccgc ccccccggga
       61 gcggcctggc atcgataatg cgccgcgccc ggggattgcg ccagattccg gccatgggtc
      121 cctccacggt tctacgctac actcccgtgc gctccttacg gggatacaac aacaatgaac
      181 agaaaccgca ccgcatccgt gctgatagca ctccaggttt tcgcatggat tgtgactcca
      241 gccgctgccg agaccgtggt ggtcagcgcc gcgcgcatgc tcgacgtcga gagaggcacg
      301 gtgacgggac cggttcaggt cgtcgtccgc gacggcctga tcgagtcgat caaccccgac
      361 cggctgccgc ccgatgcgcg cgtgctggag ctcggcgatc gcaccctgct gcccggcttg
      421 ctcgacatgc atacgcacgt gaccctggat tatttcaccg gttcgtactg ggttaccgca
      481 ccggttttcc agacggcgcc cgactgggcg atcatgggcg tgaacttcgc gggtcagaca
      541 ttgcaggcgg gcttcaccac ggtgcgggat gtcggtgcct ggccgggttt tcccgacgtc
      601 gccctgatgc gcgcgatcga tgcgggtacc gtgccggggc cggacgtttg gccggccggg
      661 cactacattt ccattaccgg cggtcactgt gacatcaccg gcttcgcgcc gggtatccag
      721 gaactggggc cgaagcaggg catcgccgac ggcgtggacg aggtgctcaa ggcggtccgt
      781 taccaggcga agcacggcgt acgggtgatc aaggtgtgcg cgaccggcgg cgtcttctct
      841 ttctccaaca atgccgcggt cggcgcgctg cagtattcgc tggaagagct cgaggccatc
      901 gtgcgcgaag cgacaaagct cggcctgaag gtggcggcgc acgcgcacgg caccgagggc
      961 atcaataccg cggtgcgggc gggggtgtca tcgatcgagc acggttcgat cctctccgat
     1021 gaatcgattg aactgatgaa agagcacggc acttatctcg tcccgaacct gtacatcaac
     1081 gacttgccgc tgccgcccga gacaccggct gccaccgttg caaagagcga ctacctggaa
     1141 cccctggtcg tgcaaagcct gcagaaagcc tacaaagccg gcgtcaacat ggcgctgggc
     1201 acggatgcgg gcgtttttcc gcacgggcag aatggccgcg agttttctgc cctggtggcc
     1261 aatggcatac cggaactgca tgcactgaaa atggcaaccg tctacgcggc cgatctcctc
     1321 ggcgtggatg accggggtgt gatcgcgccc ggcaaacgtg ccgacctcgt tgcggtcccg
     1381 ggtaacccgc tggatgacat aaaggtcatg gaggatgtgc gcttcgtgat gaaacaaggc
     1441 atggtttacc ggcaggagtg atttccggag ggaatagggt caccttgttt cagggtctgg
     1501 cgcagcgagt gcctgcttga agtgcccgat gattcatgac cccaattctt acttagttca
     1561 tccagccagt cgtgcatcgc tttgcggccc gtggcatcgc gcacgatgta ggcgaagctg
     1621 cagaaatccg aatgcggtgg cctgattctg cagaagtcgc tgagggtatg agctttacgt
     1681 gctgaagggg tctaggaatg caagcaaacg tcgataacct cattgccaca ttgagtaggc
     1741 ggaagacggg cctttttgcg ctgctaatga cggcgctcct ctcggcgctt ccacaacaga
     1801 gcgccgccga tgttgcgctg tcactcgccc acttcgcggc gggtgagagg gccttggaag
     1861 gtcgcgtggc tctccctgac gtcgaaggtg atctgacatt gcttctgcaa tgcaggacct
     1921 tcgtttccaa gcgagggagt gccaggaaca ccttctgcct ggaccacgac cgtgagttgg
     1981 aaagtctgtt ggcgaccctg cacgatgcgg tcaacaaatc ccggatgatc ccggcgatgg
     2041 tcgacggaaa gcccgaagcg gtacagatcc tctttcgcgt attgtttgtc cggcgcggta
     2101 ctcacgcgtc cgtccacgta tacgaaaact ggggacacga cgtcgataca ctgggtttcg
     2161 actaccgtgc cccccagctt tggtctcgaa aggggacgtc ctacaagtgt ttccgtcacg
     2221 atagacccct cgttgcgcaa gcgacgatcc gtatttccga aacagggctg ccgagcgggc
     2281 aggcgagcgt ggagttcgca gaaggctccg cggaggacgc cggttgcgag cagcagttga
     2341 aggaagaact agcggccagc gccttcatcc ctgccgaagc gaatggcaag ccggtggaag
     2401 ccacattcac cacggtcata ggctgggtta ctagttcgca agcgaactga cagcaccgcc
     2461 gccgccatac ccggcgggaa ccagctgaaa tcgctgacgc ccgcagccgc cagagttgcg
     2521 ccactgcagc tcgatctccg cgggcaccat cacaatcccg aacaacaggt ataccgctac
     2581 cgggaaggct aggggccgaa cgggtgcccc cgaactgcaa gtctggagag acacattaac
     2641 ttatgttagc tagtgctgct tcaacccgca ggccgtgcta tcaattctca ttagctaccg
     2701 tggcatgcgt aagagatgcg taatgtgggg gaccagtggc tcttgaaacc tttgagcaat
     2761 acggcgcgcg gttgcgggaa ctgcgtcgac tccggcaagc gaccaaagaa gaagtccggg
     2821 gagtgggcgc gaagcgccga tcgctcaccc aggacgagcg caaacaaatc gcggcgaaga
     2881 ccggctcgcg ctgccacatc tgcggcggtc cgctcgagcc cagatggcat gccgaccacg
     2941 tcctcgcgca tagcggtggc ggtcgacaca gcgtcgacaa ctacctgcca gcgcatgcga
     3001 cttgcaataa ctatcggtgg gactactcgt caacggaatt cgcggagatt ctgaagctgg
     3061 gcgtcttctt caggacggca atcgaaaagg gcgatgcaca tgcggacaag cttgctaggt
     3121 cctacctggc taaggaaagg caacgccagc gccgcatgcg atagcggcct cgcaaagttt
     3181 tcaggtctcc agtgccgtca acaggtagcg ggtggaccca tctagactct cgacaaactg
     3241 gcgaatttcg ttatcttcgc ggttgccgcg ctctcgtatc gtccaatccg gaggatagat
     3301 gggtcctgcg ccgtaccatc tatccaggag ttcgagcaac tcctgccgtg ttaccgagac
     3361 gacccagcag ccccagtcca cttgcgcgac aaccaagccc tcactatcca ggtcccgtac
     3421 ggcagaaaat agttctaacg ccgattcatc ccgtcgcggc ggaaacaagg gtccaagttg
     3481 ttgcgggacg tttccattcc aactgttctc ggtattcgtg acccattcct tcgggttgcc
     3541 aatatagacg tcgcaataag tcacttttct tgttccatca ccatcgcggc gtcaattggc
     3601 gacgtccgca gg
//
LOCUS       DASUOM010000627         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1375106,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000627 DASUOM010000000
VERSION     DASUOM010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375106"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..868)
                     /gene="gph"
                     /locus_tag="VFY27_13095"
     CDS             complement(167..868)
                     /gene="gph"
                     /locus_tag="VFY27_13095"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX5766495.1"
                     /translation="MVSPQIEPRHAILWDLDGTLIDSAGDLASALNRLLAEQGLAGLA
                     EEQVRTMIGDGAARLVERGFAAAGRPLGKSSCHDAVRRFLHLYGENPVQSTRVFDGAL
                     EVVDRLSRMGCVHGVCTNKPAAITATILDRLGLGRWVHAVVGGDSTPYKKPHPAPLLR
                     CLDELGPGLPATVVGDSEIDLLAARAVNLPVIVVTFGYSRKPVQELGADALVEHLEQV
                     PQVLSHLPLRTAGLS"
     gene            complement(878..1234)
                     /locus_tag="VFY27_13100"
     CDS             complement(878..1234)
                     /locus_tag="VFY27_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEX5766496.1"
                     /translation="MPKPIQDYKSRHNDAGSRKFGTFSYLPEMNALSIRQQVQYMINR
                     GWNCAVEHVAPGLATTDYWYMWKLPMFGERNLDTIMTELTACRNANPGNHVRIIGYDN
                     KRQTQGQSFVVYRGAA"
     gene            complement(1267..2700)
                     /locus_tag="VFY27_13105"
     CDS             complement(1267..2700)
                     /locus_tag="VFY27_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEX5766497.1"
                     /translation="MSKNEYAAGVKAYRENYWEPSYAPRESDLLACFKIVAQDGVPRE
                     EAAAAVAAESSTGTWTTVWTDLLTDLEHYRGRAYRVEDVPGDQSAFYAFIAYPIDLFE
                     EGSIVNVLTSLAGNVFGFKAIRSLRLEDIRFPLHYVMTCGGPPNGITVERDKLNKYGR
                     PLMGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRHRFEFVAKAV
                     ESAEAETGERKGHYLNVTAGTVEEMYRRAEFAKELDMPIIMHDFFTAGFSANTSLANW
                     CRNNGMLLHIHRAMHAVVDRNPHHGIHFRVLAKCLRLSGGDHLHAGTVVGKLEGDREA
                     TLGWIDCMRESFIAENRRRGIFFDQDFGHMPGMFPVASGGIHVWHMPALVAIFGDDAI
                     LQFGGGTLGHPWGNAAGAAANRTAMEACVKARNEGRDLEKEAREILVDAARHSPELEI
                     AMETWKEVRFEFDVVDKLDIKRDTRAS"
     gene            complement(2862..3767)
                     /locus_tag="VFY27_13110"
     CDS             complement(2862..3767)
                     /locus_tag="VFY27_13110"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX5766498.1"
                     /translation="MSRQHPIISVTGSSGAGTTTIRHTFHQIFLRENISAAYVEGDAF
                     HRYNRADMKRAMREADQRGNRHFSHFGPEANLFPELEAAFRNYGEMGRARLRHYVHDE
                     QEAILYGAEPGEFTEWEESPANTDLLFYEGLHGALATSDVNIAQHADLKVGVVPVANL
                     EWIQKIHRDKSTRGYTAEAVTDTILRRMPDYVHYICPQFTQTDINFQRVPTVDTSNPF
                     IARFVPTPDESIVVIRFRNPRGIDFPYLISMIKNSFMSRANSIVIPGGKMDLAMQLLF
                     TPMILRLVENRRRSIPKRTAYEYQS"
BASE COUNT          731 a         1161 c         1137 g          810 t
ORIGIN      
        1 agatggtttg ggatgcggcg accctcgacc gcttcatcga gaaccccgac gcagtcatgc
       61 cgggtaacaa catgaagccc tatagtggca tcagcgacga agagacccgt gccgagatcg
      121 tcagctacct cgaaagtgaa agtggcggcg gcggcgatac ctccgactag ctgagtccag
      181 cggtccgcaa aggcaggtgg gacagaacct gcgggacctg ttccaggtgc tcaacgagag
      241 catctgcgcc cagttcctgc accggtttcc tgctgtagcc gaaggtcacg acgatcacgg
      301 gcaaattgac ggcgcgcgcg gcaagcaggt cgatttccga gtcacccacg accgttgcgg
      361 ggaggcccgg ccccagctcg tcgaggcagc ggagcaacgg cgcgggatgc ggctttttgt
      421 acggggtgga atctcctccg accaccgcat gtacccatcg ccccagtcca agccgatcga
      481 gaatcgtcgc cgtaatggca gctggtttat tcgtgcatac gccatgcacg cagcccattc
      541 gactcaatct gtccactacc tcgagtgccc catcgaacac gcgggtgctt tgaaccgggt
      601 tctcgccgta caagtgcagg aatcgccgca cagcatcatg gcagctgctt ttcccgagag
      661 gccttcctgc cgccgcgaat cctcgctcga cgagacgcgc agctccatcg ccgatcatcg
      721 tgcgcacttg ctcttccgca aggcccgcga ggccctgctc cgcgagcagc ctgttcaagg
      781 cggatgcgag gtcgcctgcg ctgtcgataa gagtgccgtc aaggtcccaa agtattgcgt
      841 gtcgcggttc gatctgcggg cttaccacgg cgacgcgtca ggcagcaccc cggtagacca
      901 cgaaggattg gccttgcgtc tgccgcttgt tgtcgtagcc tattatcctg acgtgattgc
      961 cggggttcgc attgcggcac gcagtcagct ccgtcatgat cgtgtcgagg tttcgctcgc
     1021 cgaacatggg cagtttccac atgtaccagt agtctgtggt ggcgaggcct ggcgcgacat
     1081 gctccaccgc gcagttccag ccgcgattaa tcatgtactg aacctgctgc cggatggaaa
     1141 gggcgttcat ctccggcagg tacgaaaacg tgccgaactt ccttgagccg gcatcattgt
     1201 gacgtgattt gtagtcctgg attggcttgg gcatttgcat atctccactt tcgatgttga
     1261 tgagcatcag gacgcgcgtg tgtcgcgctt tatatccagc ttgtcaacga cgtcgaattc
     1321 aaaccgcact tccttccacg tctccatcgc gatttccagc tccggcgaat gccgcgctgc
     1381 atccacgaga atctcacggg cttccttctc gaggtcgcgc ccttcattac gcgccttgac
     1441 gcaagcttcc atggccgtac ggttggctgc cgcgccggcg gcattgcccc acggatggcc
     1501 gagggtgccg cccccgaatt gcaatatcgc atcatccccg aaaatagcca cgagagccgg
     1561 catgtgccat acgtgaatgc ccccggacgc caccggaaac atgccgggca tgtgtccgaa
     1621 gtcctggtcg aagaaaattc cgcgtctgcg attctcggca atgaagcttt cgcgcatgca
     1681 gtcgatccag ccgagcgttg cctcccggtc gccctcgagc ttgcctacga ccgtacccgc
     1741 atggaggtga tcgccgccgg acaaccgcag gcattttgcg agcacgcgga agtggatgcc
     1801 gtgatgcggg ttcctgtcga ctacagcgtg cattgcgcgg tggatatgca gaagcatccc
     1861 gttgttgcgg caccagttcg caagggatgt gttcgcgctg aagcccgccg taaagaaatc
     1921 atgcatgata atgggcatgt ccagttcttt tgcgaactct gcccgccggt acatctcctc
     1981 gactgtccct gccgtgacgt tgaggtagtg gcccttgcgc tcgccggttt cggcttcggc
     2041 gctttccacc gctttggcga cgaattcgaa acggtgtcgc catcgcatga atggctgtga
     2101 attgacgttc tcgtcgtcct tggtgaaatc gagtccgccc cgcaggcact cgtacacagc
     2161 gcgcccgtag ttcttggcgg aaagccccag cttgggtttg atcgtgcagc ccatcagggg
     2221 acgtccgtac ttgttcagct tgtcgcgctc gacagtaatg ccattcggcg gaccgccgca
     2281 ggtcatcacg tagtgaaggg ggaagcggat gtcctccagc cgcagcgagc gaatggcctt
     2341 gaagccgaat acgttgccgg caagcgacgt cagcacgtta acgatcgagc cttcttcgaa
     2401 gagatcgatc gggtaggcaa tgaatgcgta aaacgcactt tggtcgccag gcacgtcctc
     2461 gacccggtag gcgcggcccc tgtagtgttc caggtcggtc aggaggtccg tccacactgt
     2521 tgtccatgtg ccggtagagg attcagccgc cactgccgct gcagcttcct cgcgcggtac
     2581 cccgtcctgc gcgacaatct tgaagcacgc cagcaggtcc gattcccgcg gcgcatacga
     2641 cggctcccaa tagttctccc ggtaggcctt cacgcccgcg gcgtattcat tcttgctcac
     2701 gtcttaccct cctgttgcga tgttttggat gtcgacggct gcgcgggtag ccacttctgc
     2761 cgtcaatccg ggatcttgat aaagatcgcg cccggcgacc taagcaaaaa accctgcatg
     2821 ccgatgaaga cgcccgcggg gccggccatt ggttcaatct ctcagctctg atactcataa
     2881 gcagtcctct tcggaatcga cctccgccgg ttttcaacca ggcgaaggat catcggggtg
     2941 aacaggagct gcatcgcaag atccatcttg ccgcccggaa tcacgattga attggctcgc
     3001 gacatgaagc tgttcttgat catcgagatc aggtagggga aatcgatccc gcgtggattc
     3061 ctgaagcgaa tgacgacgat cgattcgtcc ggagtgggta cgaaacgggc aatgaaggga
     3121 ttggacgtat cgacggtcgg caccctctgg aagttgatgt cggtctgcgt gaactgcggg
     3181 cagatgtaat gcacatagtc gggcatgcgg cggagaatgg tgtcggtgac ggcttccgcg
     3241 gtgtagccgc gggtggactt gtcacgatga attttctgga tccattcgag gttggccacc
     3301 ggcaccacgc cgaccttcaa gtcggcatgc tgcgcgatgt tcacatcgct cgtggcgagc
     3361 gcgccgtgca gcccctcgta gaaaagcagg tccgtgtttg ccggggactc ttcccattcc
     3421 gtgaactcac cgggctcggc accatataaa atcgcttcct gttcgtcgtg cacgtaatga
     3481 cgaagccgcg ccctgcccat ctccccgtag tttctgaaag ccgcttccag ttccgggaac
     3541 aggttggctt cgggcccgaa atgactgaag tgccgatttc cgcgctggtc tgcttcacgc
     3601 attgcgcgtt tcatgtccgc gcggttgtag cgatgaaagg cgtcgccctc tacataggca
     3661 gcgctgatgt tctcgcgcag gaaaatctgg tggaaagtgt gcctgatcgt ggtggtgccg
     3721 gcgcccgagg aaccggtcac cgaaatgatc ggatgctggc gtgacatcac agccgctcct
     3781 ggcgctggaa caggccgcga ttggcaaaca gcggagacct gtcgtaatcg aggcacagc
//
LOCUS       DASUOM010000628         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_334124,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000628 DASUOM010000000
VERSION     DASUOM010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334124"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFY27_13115"
     CDS             <1..232
                     /locus_tag="VFY27_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766499.1"
                     /translation="AISFGCETYAVSLPDIEDYIGHKITVATYDPKLLPELNRFTTQR
                     RDKRPGERTGSRRPDSRRSGSRSGSRKSPRGA"
     gene            257..1060
                     /gene="cysQ"
                     /locus_tag="VFY27_13120"
     CDS             257..1060
                     /gene="cysQ"
                     /locus_tag="VFY27_13120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5766500.1"
                     /translation="MDLQQLVEPVVATATHAGAAILKVYATDFGVEAKDDESPLTQAD
                     MAAHRSIMTDLKQLVPDIPVVSEESGLPEFEVRRQWKRYWLVDPLDGTREFVKRNGEF
                     TVNIALIEEHCPVLGVVHVPVSGKTYIGVAGAGSELRLSNGERRAISVAIESASPVRV
                     VGSRSHRGKSLDAFLEKLADFEMVPMGSSLKFCLVAEGAADIYPRLGPTSEWDTAAAQ
                     AVVEQAGGTVLQLDGNPLNYNAKADILNPFFLVAGPQDHDWLGILSSVE"
     gene            1154..1372
                     /locus_tag="VFY27_13125"
     CDS             1154..1372
                     /locus_tag="VFY27_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5766501.1"
                     /translation="MTDTKIDTECFKELERLRELRISHRDLDYLIDRLSHDPLVDQLR
                     IRRLKKRRLVLKDMIAQLESELIPDLDA"
     gene            1459..1917
                     /locus_tag="VFY27_13130"
     CDS             1459..1917
                     /locus_tag="VFY27_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5766502.1"
                     /translation="MAVTININGVDHSIDATPDTPLLWALRDSLELTGTKFGCGMALC
                     GACTVHVDGAPVRSCTMPISTVSGKKVTTIEGIRSKEARAVQAAWEHHAVAQCGYCQS
                     GQIMSAAALLQSNPGPSDDDIDRAMSGNICRCATYVRIRAAIRSAAAALA"
     gene            1928..4123
                     /locus_tag="VFY27_13135"
     CDS             1928..4123
                     /locus_tag="VFY27_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5766503.1"
                     /translation="MLQLKPAAPTSRRDFLKALGIAGAGLTIGVSLSPPEAAEAALPS
                     KVKPEASFIPNAFIRINPDNTINVVIKHLEMGQGTYTGLATLVAEELDADWNQVIAEG
                     APADVSRYNNLLMGPTQGTGGSTAIANSFEQMRIAGATAKAMLVAAAASKWEVPAESI
                     STSGGVLSHKPSGRSASYGELSSLAAEQPVPDPGSLKLKEAKDFRYIGKSMSRKDIGK
                     TDGTAIFTQDVKMEGMLTALVLHPPRFGAKVKAVRDQRTRDSRGVVDVVQIPGGVAVL
                     ATDFWSARKGRDLLEVDWDESAAMMRGSSDLLEEYRALAAKPGQVARNDGDADKALSG
                     AARVIEATYEFPYLAHATMEPMNCVVRVGNDSCEIWNGAQLHTVDQAMVASVLGMPDT
                     QVRINTLYAGGSFGRRANPHSDYVVEAAYIAKARKGTPVKLVWTREDDTRAGYFRPMY
                     VHRLRGGLDANGNPVAWEQRVVGQSILKGTPFEQFIQDNLDTSSVEGASTLPYGIPNL
                     RVDLHTVDPGIPVQWWRSVGHTHTAYSTETFIDQLAAAAGRDPVEFRVALLKDHPRHL
                     GVLKLAAGKANWGSALPKGHARGIAVHESFNSFVAEVAEVSLQSDGGFKVERVVCAVD
                     CGIAVNPDIIVAQMESGIGYGLSPLLMSEITLDDGKVVQSNFHDYQVLRINQMPKVEV
                     HIVPSAEPPTGVGEPGTPPVAPAVANALFAATGKTYHRLPLRLDEGVSA"
     gene            complement(4137..>4544)
                     /locus_tag="VFY27_13140"
     CDS             complement(4137..>4544)
                     /locus_tag="VFY27_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766504.1"
                     /translation="MFCTGGIRCEKASALMLELGFREVFQLQGGILNYLEQVAPEDNR
                     WTGECFVFDSRVAVDRDLAEGGYVQCHACRRPLSSEDLASPGYREGVSCPRCIDELDA
                     ERAVRLEERRRQVELARERGDRHIGKRPDREPQ"
BASE COUNT          937 a         1420 c         1415 g          772 t
ORIGIN      
        1 tgccatcagc ttcggctgcg agacctatgc agtgtccttg ccagacatag aggattacat
       61 cggccacaag ataaccgtgg ccacttacga cccgaagctg ctacccgaac tcaaccggtt
      121 tacgacgcag agacgggaca aacgcccggg cgagcggacg ggctcacgac gcccggacag
      181 caggcgatcc ggcagccgca gcggtagccg aaaaagcccg cgcggtgcat aagagctgaa
      241 acaggaacaa catagaatgg atctccagca actcgttgaa ccggtggtcg cgacagcgac
      301 gcacgcgggc gctgcaatac taaaggttta cgcaaccgac ttcggcgtcg aggccaagga
      361 tgatgaatcg ccgctcacgc aagcggacat ggcggcgcac cgctctatca tgacggacct
      421 caagcaactc gttcccgaca ttccggtggt ctcggaggaa agtggcctgc ccgagttcga
      481 ggtgcgccgc caatggaagc gttactggct ggtcgatccg ctggacggca cccgagagtt
      541 cgtcaagcga aacggcgaat tcaccgtcaa catcgcgctg atcgaggagc attgcccggt
      601 gctcggcgtc gttcatgtcc cggtatccgg caagacctac atcggcgtcg ccggggcggg
      661 ttccgaactc cgattgtcca atggtgagag gcgcgccatc agcgtcgcga tcgaaagcgc
      721 gagcccggtg agggtggtcg gcagccgttc gcatcgcggc aaaagcctgg acgcttttct
      781 cgaaaagctg gccgatttcg agatggtgcc gatgggcagc tccctgaagt tctgcctcgt
      841 cgccgaaggc gccgcagata tttatccgcg cctcgggccg acctccgaat gggacaccgc
      901 ggcggcacag gcagtggtcg aacaagccgg tggcacggtg ctgcagctgg acggtaatcc
      961 gttgaactac aatgcaaaag cggacatcct gaatcccttc ttcctggtcg caggtccgca
     1021 ggaccacgat tggctcggca tactttcctc ggtcgagtaa caaagagttg taactgagct
     1081 aatattgcga ctgacgaaga ccgtcatccg gcaaaggggt tcggcgagag ttcagccaat
     1141 cagctcaggg tttatgaccg acaccaagat cgatacagag tgcttcaagg agctcgagag
     1201 actcagggaa ctgcgcatca gtcaccgtga tctcgattac cttatcgaca ggctgtcaca
     1261 cgacccgctg gttgaccagt tacgcatccg gcgcctgaaa aagcgtcggc tggtcctgaa
     1321 ggacatgatc gcccagctcg aaagtgagct catccccgac ctcgacgcct gactgccggt
     1381 catccccgct cgtgcgggcc gcctcgcttt ccgcgcgcga ccgcacacct gcacgcaaac
     1441 ctcgaggaga gaataaagat ggccgtcacg atcaatatca atggcgtcga ccattccatc
     1501 gatgcaaccc cggacacgcc gctcctatgg gccctgcgcg actcactcga gctgacgggc
     1561 accaaattcg gctgcggcat ggcgctttgc ggcgcatgca ccgttcatgt ggacggcgcg
     1621 ccggtgcgct cctgtacgat gccgatctcg acagtaagcg gcaagaaggt caccaccatc
     1681 gagggcatcc gttcgaaaga agcaagggcc gtgcaggctg cctgggaaca ccacgcggtg
     1741 gcgcagtgcg gctactgcca gtccgggcag atcatgtccg cggcggcact tctgcagtcc
     1801 aatccgggac cgagcgacga cgatatcgac cgggctatgt ccggcaatat ctgccgctgc
     1861 gccacctatg tccgcatccg cgccgcgatc aggtcggcgg ccgcggccct cgcataagga
     1921 gtcgaacatg ttgcaattga agccagcggc gccgacatcc cggcgcgact tcctgaaagc
     1981 actcggcatc gccggcgcag gactcacaat aggcgtttcg ctgtcgccgc cagaagcggc
     2041 cgaagcggct cttccgtcga aggtgaagcc ggaagcttcc ttcataccca atgctttcat
     2101 ccgcatcaac ccggacaaca ccatcaacgt cgtgatcaaa cacctcgaga tgggccaggg
     2161 cacctacacc ggcctggcga ccctggtggc ggaagaactc gatgccgact ggaaccaggt
     2221 catcgcggaa ggggccccgg cggacgtctc ccgctacaac aacctgctga tgggtcctac
     2281 tcagggcacg ggcggtagta ccgcgatcgc caactcgttc gagcagatgc gcatcgctgg
     2341 agccactgcg aaggcaatgc ttgtcgccgc tgctgcttcg aagtgggaag tacctgcgga
     2401 gagcataagc acctccggcg gggttctctc gcacaagcct tccgggcgca gcgcaagcta
     2461 cggcgaatta agctcgctgg ctgcggagca gccggtgccg gatccgggct cattgaagct
     2521 caaggaagcg aaggatttcc gctacatcgg caaatcgatg tcccgcaagg atatcggcaa
     2581 gacggatggc accgccatct tcacgcagga cgtcaagatg gagggcatgc tgaccgcgct
     2641 ggtgctgcac ccgcctcgct tcggcgcgaa agtgaaagcc gtcagggacc agcgaacgcg
     2701 cgattcccgt ggcgtcgtcg atgtggtgca gataccgggc ggcgtcgccg tgctcgccac
     2761 cgatttctgg agcgccagga aaggacgcga cctgctcgag gtggactggg acgaaagcgc
     2821 cgccatgatg cgcgggagtt ccgatctgct ggaagagtat cgcgccctcg ctgcgaaacc
     2881 ggggcaggtg gcacggaacg acggcgacgc ggacaaagca ctgtcgggcg cggccagggt
     2941 gatagaagcg acttacgaat ttccctacct tgcgcatgcg acgatggagc cgatgaactg
     3001 cgtcgtcagg gtcgggaacg attcctgcga aatctggaac ggcgcccagc tgcacacggt
     3061 cgaccaggcg atggtggcgt cagtactggg tatgccggat acgcaggtca ggataaatac
     3121 cctctacgcg ggcggcagct tcgggcggcg cgcgaacccg cattccgatt acgttgtcga
     3181 agcggcttac atcgccaaag ccaggaaggg caccccggta aagctcgtct ggacgcggga
     3241 agacgacacc cgcgcgggct acttcaggcc gatgtacgtc caccgccttc gcggcggact
     3301 ggatgcgaac ggcaacccgg tagcgtggga acagcgcgtg gtcgggcagt cgatcctgaa
     3361 gggcacgccc ttcgagcaat tcatacagga taacctcgac acgtcctccg tcgagggtgc
     3421 gtccaccctg ccctacggga ttccaaacct gcgcgtcgac ctgcataccg tcgacccggg
     3481 tattccggtg caatggtggc gttccgtcgg ccacacgcac accgcctact cgaccgaaac
     3541 cttcatcgac cagcttgcag cggcggccgg acgcgacccg gttgagttcc gcgtggctct
     3601 actgaaagac catccccggc acctgggcgt gctgaaactg gctgccggga aagcgaactg
     3661 gggctctgcg ttgccgaagg gacacgccag gggtatcgcc gtgcacgaat ctttcaactc
     3721 cttcgtcgct gaagtcgccg aggtttcttt gcagagcgac gggggtttca aggtcgagcg
     3781 ggtcgtatgc gccgtagact gcggtattgc cgtcaatccg gacatcatcg tcgcgcagat
     3841 ggaaagcggc atcggctatg gcctgtctcc gctgctcatg agcgaaatca cgctggacga
     3901 cggcaaggtc gtacagtcga actttcacga ctaccaggtg ctgcgcatca accagatgcc
     3961 caaagtcgaa gtgcacatcg taccgtcggc ggagccgccg accggcgtcg gtgaaccggg
     4021 cacgccgccg gtcgcccccg ctgtcgccaa cgcgttgttc gctgctaccg gcaaaaccta
     4081 tcaccggttg ccgttgaggc tggacgaggg cgtctcagca taggcaagag agaggactac
     4141 tgcggctcgc ggtccggccg cttgccgata tgccggtcac cgcgttcgcg ggcgagctct
     4201 acctgtcgac ggcgttcttc caggcgcacg gcgcgttcgg cgtcgagctc gtcgatgcaa
     4261 cgcgggcagg aaacgccctc gcggtagccc ggtgaggcaa ggtcttcgct ggacagcggc
     4321 ctgcggcagg cgtgacattg cacatacccg ccttcagcca ggtcccgatc gacggcaacg
     4381 cgactgtcga agacgaagca ttcacccgtc cagcggttat cttccggcgc gacctgttcc
     4441 aggtagttga gtattccgcc ttgcagctgg aagacctcgc gaaaaccgag ctccagcatc
     4501 agtgcagagg ccttctcgca gcggatgccg ccggtgcaga acat
//
LOCUS       DASUOM010000629         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_574287,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000629 DASUOM010000000
VERSION     DASUOM010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574287"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..697
                     /locus_tag="VFY27_13145"
     CDS             32..697
                     /locus_tag="VFY27_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEX5766505.1"
                     /translation="MIRLFLLSVCLFAAAATRAEPGWVSDQIEVTLRTGPSTSNAITR
                     MLPSGTQIEIVERDDDTGYARVRTSSGAEGWVLTRYLMNEPPARQQLERLTSQLNTAT
                     TEGSSLGSQLTAIKSQYDQASATIASLERDKANLERELNEIKQTASNTIEIDRQNRDR
                     RQQLADAEIKVSILQQENESLSGQTTRNWFLAGALVLFIGVVMGLWLPRIRWQKRSRY
                     ERF"
     gene            complement(847..3210)
                     /gene="ppsA"
                     /locus_tag="VFY27_13150"
     CDS             complement(847..3210)
                     /gene="ppsA"
                     /locus_tag="VFY27_13150"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX5766506.1"
                     /translation="MEPYVIELEKLGMEDIESVGGKNASLGEMIGKLGALGVRVPSGF
                     ATTAAAYRDFLKIDALDERIRKRLAALNVDDIPALSDAGRTIRNWITETPLPDRLMRD
                     IETAWGKMSKGNDIRVAVRSSATAEDLPDASFAGQQETFLNVRGVDHLVQALHQVYAS
                     LFNDRAIAYRVHQGFDHALVALSVGIQHMVRSDIGAAGVMFTLDTESGFRDAVFITSS
                     YGLGETVVQGAVNPDEFYVYKPALESGKFPILRKNLGSKAIKMVFSDNHVPGKTVETV
                     DVEESESLKFSLTESEITELARTAVTIEKHYRRPMDIEWGKDGKDGQLYILQARPETV
                     QSRTGRTIQRFRLIDRSKILSKGRSIGQKIGAGTAKVIRDVSEMERIQPGDVLVSDMT
                     DPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATTGIQDGKDVTVSCA
                     EGDAGFVYEGRLQYEEKEIELDALPPIAVKIMMNVGNPDRAFDFANIPNHGVGLARLE
                     FIINRMIGVHPNALLEYDKQNADTREAIDEQMAGYDDPVRFFVDKLAEGISTIAAAFA
                     PEPVIVRLSDFKTNEYANLIGGEAYEPKEENPMLGFRGAARYVSENFRPCFELECAAL
                     RKVRNDMGLTNVQVMVPFVRTVKQGKEVIGLLEDNGLVRGVDGLKIIMMSELPTNALL
                     ADQYLDDFDGMSIGSNDMTQLTLGLDRDSALIADLFDERDEAVKALLAMTIEACRKRG
                     KYVGICGQGPSDHPDLAKWLLAQGIESVSLNPDTVVETWMFLAGQAV"
     gene            3371..4195
                     /locus_tag="VFY27_13155"
     CDS             3371..4195
                     /locus_tag="VFY27_13155"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX5766507.1"
                     /translation="MSERTVFFLSDQTGVTAETLGHSLLTQFDSQDFRQVTLPFIDTD
                     DKAREAVRKINEAARNASLKPIIFSTLVQDEFRAIVKAADGLHLDIFDVFLEPLEQEL
                     KQKSTHTAGRAHGMSDIEGYMKRIEATNFALANDDGGMTRNYEMADVILVGVSRSGKT
                     PTCLYLALQYGVYAANFPLTDEEFDSGTLPEFLVSQKSKLFGLTIAPARLRQIRKERR
                     ALGRYATAQQVRFELREAEKIFKRYDIPFVDTTEFSIEEISSRILDSTGVERRVRP"
     gene            4288..4743
                     /locus_tag="VFY27_13160"
     CDS             4288..4743
                     /locus_tag="VFY27_13160"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEX5766508.1"
                     /translation="MLLDYQLVPQTIVKPRSFVGLMLLYESNFLRLKHVIPELDRLDG
                     HYKSRVAGDCDLHLEILDRSKYTITLSLSYFFNDKGVRIADPDMKIRAYLDGHLAEVM
                     SLGGDHRHAEIRRLTSEHRGELGTRWRRNIVLNKWLEYLMHQGHLILER"
     gene            4866..>5184
                     /locus_tag="VFY27_13165"
     CDS             4866..>5184
                     /locus_tag="VFY27_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766509.1"
                     /translation="MSSLAAMFGSPREKVQDSEKLLHLYWNRAELKKQFAGMRKAQYK
                     LQDRLKQQEGETARLQQKLEHLEALLLDPEWAGNAVVFYQLRGLAFRCQRKLSKFAEQ
                     LKQQ"
BASE COUNT         1114 a         1525 c         1482 g         1063 t
ORIGIN      
        1 ttgggtggga tgactgaaca tgatcagacc ggtgatacga cttttcttac tgagcgtgtg
       61 ccttttcgca gctgcggcga cccgcgcgga acccggctgg gtcagtgacc agatcgaagt
      121 taccctccgc acgggaccaa gcacctccaa cgccattacg cgcatgctgc ccagcggcac
      181 gcagatcgag atcgtcgaac gtgacgatga caccggctac gcgcgcgttc gtacctcgtc
      241 gggcgccgaa ggctgggtgc tcacccgcta cctgatgaac gaaccgcctg cgcgacaaca
      301 gcttgaacgg ctgaccagcc agctcaatac ggccaccact gaaggcagca gccttggatc
      361 gcagctgact gcgatcaaat cgcagtacga ccaggcgtca gcaacgatcg cttccctcga
      421 gcgagataag gcgaacctgg agagggagct caacgagata aagcaaaccg cttccaacac
      481 aatcgagatc gaccgccaga acagggaccg tcgccagcaa ctggccgatg ccgaaatcaa
      541 ggtcagcatt cttcaacagg aaaacgaatc cctctcgggc cagaccacgc gaaactggtt
      601 cctcgcaggc gcactggtgc tgttcatagg tgtcgtcatg ggcctttggc tgccgcgcat
      661 ccgctggcag aaacgatcac gctacgagcg cttctgaatg tagaaggtcg cctgagcgga
      721 catcgcggag atcggatggg gtttcggtga cgcgttcgac cggcatcagt gggagcccgc
      781 cacgcgggcg agttcgctaa cacgatcgaa ctcgcccgca tggcgggctc ctacaggtgc
      841 ttgtttctag acggcctggc cggccaggaa catccaggtc tccactaccg tgtccgggtt
      901 caaggagacg ctctctatgc cctgcgcgag cagccacttc gcaagatcgg gatgatcgga
      961 aggcccctgg ccgcaaatac cgacgtattt cccccgcttc ctgcacgctt caatggtcat
     1021 cgcgagcaac gccttgactg cctcgtcgcg ttcgtcgaaa agatcggcga tgagagccga
     1081 gtcgcggtcg aggccaagcg tgagctgggt catgtcgttg gagccgatcg acatcccgtc
     1141 gaagtcgtcg aggtactggt cggcaagaag tgcgttggtc ggcagctcgg acatcataat
     1201 gattttcagt ccgtccacgc cgcgaaccag gccgttgtct tccagcaggc cgataacttc
     1261 cttgccctgt ttcacggtcc tgacgaaagg gaccataacc tgcacgttgg tgaggcccat
     1321 atcgttgcgt accttccgca gcgcagcaca ttccagctcg aagcaggggc ggaagttctc
     1381 tgatacatag cgtgccgccc cgcggaaccc cagcatcggg ttttcctctt tcggctcgta
     1441 cgcctccccg ccgatcaaat tggcgtactc attcgttttg aagtccgaca gccgcacgat
     1501 gacaggttcc ggcgcaaagg cggcggcgat cgtgctgatg ccttcggcaa gcttgtcgac
     1561 gaaaaagcgc actggatcat catatcccgc catctgttcg tcaatcgctt cccgggtatc
     1621 cgcattctgc ttgtcgtact cgagcagggc gttgggatgc acgccgatca tcctgttgat
     1681 gatgaattcc agtcgggcga gtcccacacc atggttcggg atgttcgcaa agtcgaacgc
     1741 gcgatcggga ttgccgacgt tcatcatgat cttgaccgct atgggtggca gggcatccag
     1801 ctcgatttcc ttttcttcgt actgcaaacg gccctcatat acaaaaccgg catcgccttc
     1861 ggcgcagctc acggtaacgt ccttgccgtc ctggatgcca gtggtggcat cgccacagcc
     1921 caccacggca gggataccca gttccctcgc gatgatcgcc gcatggcagg tgcgtccacc
     1981 acggttcgtg acaatagccg aggcgcgctt catcaccggc tcccaatcag ggtctgtcat
     2041 gtcggacacc aggacgtcgc ccggctggat acgctccatc tccgacacat cgcggatgac
     2101 cttggcggtt ccggcgccga tcttctgtcc tatgctgcgt cccttgctca ggatcttgga
     2161 gcgatcgatc aggcgaaatc gctggatcgt gcgcccggtg cggctctgca ccgtctcggg
     2221 tctcgcctgc aggatataca gctggccgtc tttcccgtcc ttgccccact cgatatccat
     2281 cgggcggcgg tagtgctttt cgattgtcac ggcggtgcgc gcgagttccg taatctcgct
     2341 ttccgtgagg gagaacttca ggctctctga ctcctcgacg tcgaccgttt caacggtctt
     2401 gccgggaacg tgattgtcgc tgaagaccat cttgatggcc ttgctgccaa ggttctttct
     2461 caggatcgga aacttgccgc tctcgagcgc cggcttgtaa acgtagaact cgtcggggtt
     2521 taccgcgcct tggacaacgg tttccccaag accgtaggat gaggtgatga atacggcgtc
     2581 cctgaagccg gactcggtgt ccagcgtgaa catgacgcct gccgccccta tgtcgctgcg
     2641 caccatgtgc tggatgccga cggaaagcgc caccagggca tgatcgaagc cctggtggac
     2701 gcggtacgcg atggcccgat cattgaaaag agaggcatat acctggtgca aggcctggac
     2761 gaggtgatcc acgccacgaa cgttcaggaa tgtttcctgc tggcctgcga aggaggcatc
     2821 gggaaggtct tcggcagtgg cagaagagcg tacagccacc cggatatcat tgcccttgga
     2881 catcttgccc caggcggttt cgatgtcacg catcagacgg tcgggaagcg gcgtttcggt
     2941 aatccagttg cgaatcgtcc tgcccgcatc cgaaagagca gggatatcgt cgacgttgag
     3001 ggccgcgagc cgcttcctga tgcgctcatc gagcgcgtcg atcttcagga aatcgcgata
     3061 ggccgccgcg gttgtggcga atccgctcgg cacgcgcaca cccagtgcac cgagtttgcc
     3121 gatcatctcc ccaagcgagg cgttcttccc gccaaccgat tcgatatcct ccatgccgag
     3181 cttttcaagc tcgatgacgt acggctccaa aatcgactcc ctgtgagtgt ttccgaacga
     3241 ggaaatagtc aatcaacaat gctagcccat tgggcgatgg caggcgagaa cgtcacggcc
     3301 ctttctggcg gtaggtcacc caagtacact gaacccggat caagccagat ggccacccgg
     3361 gacacaagca atgagcgaac gtactgtatt ttttctgtcg gaccagaccg gcgtcacggc
     3421 agaaacgctg gggcacagcc tgcttacgca gttcgacagc caggattttc ggcaggtgac
     3481 tttgccattt atagataccg atgacaaggc gcgggaggcg gtacgcaaga taaacgaggc
     3541 ggcccgtaac gcctcgctga aaccgatcat tttctccaca ctggtacagg atgagttccg
     3601 cgcgatcgtc aaggcggcgg acgggctgca cctggatatc ttcgatgttt tccttgagcc
     3661 gctggaacag gaactcaaac aaaagtctac gcataccgcg ggccgcgccc acggtatgag
     3721 cgacattgag ggttatatga aacggatcga ggctaccaac tttgcgctgg cgaacgatga
     3781 cggaggcatg acccgcaact acgagatggc tgacgtcatc ctggtcggcg tatcgcgcag
     3841 cgggaagaca ccgacctgcc tttaccttgc cctgcagtac ggtgtttacg ctgcaaattt
     3901 tccactgacc gatgaagaat tcgacagcgg cacgttgccg gaattcctgg tgtcgcaaaa
     3961 atcgaagctg ttcggcttga ccatagcccc ggcccggttg cggcagatcc ggaaagaacg
     4021 gcgggcgctg ggtcggtatg ccaccgcaca gcaagtgcgg ttcgaactgc gggaagcgga
     4081 gaaaatattc aagcggtatg acataccgtt cgtcgatacg accgagtttt ctatcgagga
     4141 aatctccagt cgcattcttg atagtactgg cgtggagcga agggtgcgac cctgattttc
     4201 accttgaaag ccagtgcata attcgcaaaa agtggaactg taattgcata ccttctgaat
     4261 caaatttgct gtgatatatt gatggccatg ttactggact atcaacttgt accgcagaca
     4321 atcgtcaagc cgcgaagctt tgtcggtctg atgttgttat acgaaagcaa tttcctgcga
     4381 ctgaaacacg tcattcccga acttgatcga ctcgatggac attacaagtc acgcgttgcg
     4441 ggcgactgtg acctccacct cgaaattctc gatcgcagca agtacacgat aaccttatcg
     4501 ctgtcgtatt ttttcaacga caagggtgtc cggattgccg atcccgacat gaagatccgc
     4561 gcatatctcg atggccacct cgccgaggta atgagcctcg gcggcgatca ccgtcacgca
     4621 gaaattcgtc ggcttacctc tgagcatcgt ggcgagctcg gtacgcgatg gaggcgcaac
     4681 atcgtgctga acaaatggct cgagtacctg atgcaccagg gacacctcat cctcgaacgc
     4741 tagcaccgct caaccgaaaa ctgtcgattc aatcaaggat cggacagggg ccaattgcca
     4801 tcattcgcat cgtcccgtaa caccgccggc tgtggctgcg tcaggattat gtaaaggagt
     4861 ggtgcttgtc gagtctggca gcaatgttcg gcagtccgcg ggaaaaggtg caggacagcg
     4921 agaaattgct gcacctgtac tggaatcgcg ccgagctcaa gaagcagttc gccggcatgc
     4981 gcaaggccca gtacaagctc caggaccggc tcaagcaaca ggagggcgag acggcgcgtc
     5041 tgcagcagaa gctcgagcac ctcgaagctc tgttgctaga cccggaatgg gccggcaatg
     5101 cagtagtctt ctaccaactg cgtggcctgg cattccgctg ccagcgcaag ttgtcgaagt
     5161 tcgccgagca gctaaagcag caac
//
LOCUS       DASUOM010000630         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1420252,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000630 DASUOM010000000
VERSION     DASUOM010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420252"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="VFY27_13170"
     CDS             complement(<1..1964)
                     /locus_tag="VFY27_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766510.1"
                     /translation="MSIFEELKRRNVIRMGIAYAVASWVLLQVVDVISPAIDLPVWVP
                     KLVLLILGIGLIPVLVFAWAFEITPDGLKKEADVDRTNSIASATGRKLNYVITGFLVA
                     AIALLLVDRQMRPDAASAGDTELAAAAAERDGSIAVLPFVNMSSDEEQEFFSDGITEE
                     ILNSLASVKELKVAGRTSSFAFKDQNDDLRRIGDSLGVQHILEGSVRKSGDKVRITAQ
                     LIQVDNGFHLWSETYDRELTDVFAIQDEIANEILKQLRAQLVGANESVAETMRTSPEV
                     YELYLRAKQRIYTRDHDEIRTAIEELDSAIRLDGAYAPAYAQRGIATILSSDQQYGAI
                     PHQEARRRGKRYIDEALRLDPESAEAWAGLGLYHDRDSEAAAEALVKALTINPNLIDA
                     SNWLQISLRSSGQMRSSLDILRDIAERDPLYRPAFSNAMQLFDAFGLDDEADKLLKRI
                     EAFNPDNPALYLARATRYMYAGEMGKGLQQLEEASKHGPMSGVEQVFLSIGLLQTGQP
                     ERALTEGSVYFRPDALYALDRKDEAIALAYEHARGGDPESLFYMLVIENRNEELVDYL
                     EERWPSLAAFATENPGGDNGYSLMMHVALAYRRVGDETLADEALELVDTRMQLLEREG
                     IDNRIFTTSKASYLAMRSDVDGALDHLEAAV"
     gene            complement(2177..3373)
                     /locus_tag="VFY27_13175"
     CDS             complement(2177..3373)
                     /locus_tag="VFY27_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEX5766511.1"
                     /translation="MALTVAAGAIVDEPPGPLLFGIRVEFFLFALTLLGVALFHDRTL
                     DVGFIGLASIVLLKYLTVTDFRLARHAWEEAPILVNLLGLLLGFAVLARHFEQSDIPE
                     ILPRFLPDDWKGGFTLLAMVFVLSAFLDNIAAAMIGGTVALHVFHRRIHVGYLAAIVG
                     ASNAGGSGSVLGDTTTTMMWIEGVSAVDVLRAYVAAIPAFLFFAVVASRQQHAHHPIQ
                     SQPVGAHRIIWARVVAVILILAGAIATNILLDFPAAGVWIAIAISAFFVRTDWKVVPA
                     SMKGTLFLLTLVTCASLMPVETLPVASWQTAFGLGWVSAVFDNIPLTKLALEQDGYDW
                     GMVAFAVGFGGSMIWFGSSAGVALANMVPEARSVVNWIRHGWHLGVGYTLGFAVLLLV
                     HGWHPG"
BASE COUNT          702 a         1042 c         1035 g          646 t
ORIGIN      
        1 actgctgctt ccaggtggtc aagcgcgcca tcgacatcgc tacgcattgc gagataagac
       61 gctttgctcg ttgtgaagat ccggttatct atgccctctc tctcgagcag ctgcatgcgc
      121 gtatcgacta actcgagcgc ctcatcggca agcgtctcgt cgccgacacg gcgatacgcc
      181 agggcgacat gcatcatcag gctgtagccg ttgtcgccgc cgggattttc ggttgcgaac
      241 gcggccaggc tgggccagcg ttcttccagg taatccacca gttcctcgtt acgattctcg
      301 atcaccagca tgtagaacag ggattcggga tcgccaccgc gggcatgctc gtacgcaagc
      361 gcaatcgcct cgtctttgcg atctaacgca tacagcgcgt cgggacggaa atagacggag
      421 ccctcggtaa gggcccgctc cggttgcccg gtttgcagca gtccgatgct gaggaaaacc
      481 tgctcgacgc cgctcatggg accgtgcttc gacgcctcct ccagctgctg cagccccttg
      541 cccatttcac cggcgtacat gtagcgcgtc gcacgggcaa gatacagggc cgggttgtcc
      601 ggattgaagg cctcgatccg tttcaagagc ttgtccgcct cgtcgtcgag gccgaacgcg
      661 tcgaagagct gcatcgcgtt gctgaaggca ggccggtata gcggatcgcg ttccgcgata
      721 tcgcgcagga tgtccagcga gctgcgcatc tgcccggaac tccgcaatga aatctgcagc
      781 cagttgctgg catctatcag gttcgggttg atcgtgagcg ccttgacgag ggcctctgcc
      841 gctgcttcgg aatcccggtc gtggtagagg ccgagtccgg cccaggcctc cgctgattcg
      901 gggtcgagac gcagcgcttc atcgatatag cgcttgccgc ggcggcgcgc ttcctggtgc
      961 ggaatcgccc cgtattgctg atcggacgaa agtatggtcg cgatgccccg ctgggcgtag
     1021 gcgggagcat acgcgccgtc gaggcgaatg gcgctgtcga gttcttcgat agccgtccgg
     1081 atttcatcgt gatcccgcgt gtagatgcgc tgcttcgccc gcaggtacag ctcgtagacc
     1141 tccggggacg tgcgcatcgt ctcggcgacg gactcgttcg cgccaaccag ctgcgcccgc
     1201 agttgcttga gaatctcgtt cgcaatctcg tcctggattg cgaacacatc cgtcagctcc
     1261 cggtcgtagg tttccgacca gaggtgaaag ccgttgtcta cctggatgag ctgggcagta
     1321 attcgcacct tgtcgcccga cttgcgtacg gaaccttcga gaatgtgctg cacgcccagc
     1381 gagtcgccga tgcgccgcaa atcgtcgttc tggtctttga acgcaaagga agacgtacgg
     1441 cccgcgactt tcagttcctt cacggatgcc agcgaattaa ggatttcctc ggtgatgccg
     1501 tccgagaaaa attcctgttc ttcatccgag ctcatgttga cgaacggcaa caccgcgatg
     1561 gacccgtcgc gctctgctgc tgccgccgcc agctcggtat cgccagccga cgctgcgtcc
     1621 gggcgcatct gccggtccac cagcagcagc gcgatcgcgg cgacgaggaa gccggttatc
     1681 acgtaattca gcttgcggcc ggtagcgctt gcgatggaat tcgtgcggtc aacgtccgct
     1741 tcctttttca atccgtcagg ggttatctcg aacgcccagg cgaagaccag caccggtatg
     1801 agcccgatcc cgagtatcag gagcaccagt ttcggtaccc atacgggcag atcgatggct
     1861 ggcgaaatga catcgacgac ctgcagcagc acccaggagg ctacggcata agcaataccc
     1921 atccggatga catttcggcg cttcagttct tcgaatatgg acactggggt tccccgccgg
     1981 actcttgtta ttgtgctgtg atagtaccaa ttcccgcgac tccatgggga ctccgagggc
     2041 ggtgactgca actggcagcg cgacagcaaa tggggatagt gatctggtct ttccgcgttt
     2101 ttcgtggatc ccttcaaact tccgagtgag agggtaaaaa ccaactgcgc cagtgcacaa
     2161 catgcgcagg cggttatcag ccaggatgcc atccgtgaac aagcagcagg accgcgaaac
     2221 ccagcgtgta gcctacgccg aggtgccagc cgtggcggat ccagttcacg acggacctcg
     2281 cttcagggac catgttcgcg agcgcgacgc ccgcggacga cccgaaccag atcatcgagc
     2341 cgccgaagcc gactgcgaat gccaccatcc cccagtcata gccgtcctgc tcgagcgcaa
     2401 gtttcgtgag cgggatgttg tcgaacacgg cggataccca gcccaagccg aacgccgtct
     2461 gccaggaagc gaccggcagc gtttctaccg gcatcaacga ggcgcacgta acgagcgtaa
     2521 gcaggaacag ggtgcctttc atactggccg gaacgacttt ccagtccgtg cggacaaaaa
     2581 atgctgagat cgcaattgca atccacaccc cggccgccgg gaagtccagc aggatgttcg
     2641 tcgctatggc gcctgcgagg attagaatca cggcgacaac cctggcccag atgatcctgt
     2701 gtgcacccac gggttggctc tggatcgggt ggtgggcgtg ctgctgacgt gaagcgacta
     2761 ctgcgaagaa caggaatgcc gggatagcgg cgacgtaggc acgcagtaca tcaactgcgg
     2821 agacgccttc gatccacatc atcgtcgtgg tcgtatcgcc gagcacggat ccggagccgc
     2881 cggcgttact tgcaccaacg attgcggcga gataaccgac atggatgcgc cggtgaaaca
     2941 cgtgaagagc caccgtgcct ccgatcatgg cggcggcgat gttgtccagg aaggcggaaa
     3001 gcacgaacac catggcaagc aacgtgaagc cgcccttcca gtcgtcgggc aggaatctcg
     3061 gcaatatctc ggggatgtcg gattgctcga aatgacgggc aagcacggcg aagccgagca
     3121 gcaagccgag gaggttgacg agaatcggcg cctcctccca tgcatggcgc gccagcctga
     3181 aatccgtcac cgtcaggtac ttgagcaaaa cgatcgacgc aaggccgatg aagcccacgt
     3241 cgagggtgcg gtcgtggaac agcgcgacac ccaggagcgt caaagcaaag aggaagaact
     3301 ctacccggat gccgaacaat aacggcccgg gtggttcatc gacgatcgca ccggccgcca
     3361 cggtaagggc cataacggca acgcccatcc aggccagcga tttgcgggtc agcaacattc
     3421 agtat
//
LOCUS       DASUOM010000631         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1487569,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000631 DASUOM010000000
VERSION     DASUOM010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487569"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFY27_13180"
     CDS             complement(<1..741)
                     /locus_tag="VFY27_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5766512.1"
                     /translation="MIPSALTPNEERVLVFAPFGADGELAQRVLAELEIRAQVCDSIV
                     SLCAELEEGVGAVLITEEALWDPALVSLIDRFDDQPSWSSIPIVIAANIDLELYKREH
                     ILRRLEGYKVTFLSRPVVVVSLASTLRSALAARRRQYQVRDLMLNLHTELRLRDEFLA
                     TLSHELRNPLSAVRNAVQLMNMSEIEDPAVAKSRDIIDRQTLDLSRLLDDLLDVARVT
                     QGKISLKVEELSLRRLLEEIVRDQREAGT"
     gene            complement(749..>2042)
                     /locus_tag="VFY27_13185"
     CDS             complement(749..>2042)
                     /locus_tag="VFY27_13185"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5766513.1"
                     /translation="LAESHDEMQQVAESHGWDLSGVKLFAPQTGDAGLPRDQYTLFHP
                     GEVELTDLITRITDEIRESRPARVVVDSLADIRLLARDPLILLRQMRLLRDTLTDLHC
                     TVLLLNNLQRGNEHSGSLETFAHGIVELEYSDTLYGATRRCISIIKLRGMRYVSGRHD
                     AKITTGGLMVYPRLVPHASVGPVDTPLISSGSDELDLLCGGGLESGSSAMLIGSAGTG
                     KSTVAAQFALAAAERGDSTSMFLFDETDRSCRLRWEGMNMPVMQYVEQGLIRTCSVDP
                     TDVSPGEFCHIVRESVEQNETRIVVIDSLSGYMQVMPQSRFMEVHLFELLKYLNSRNV
                     LSILIVTQHGILGSALESPIDVSYLIDTILLMRFFEANGEVHKAISVVKKRLGKHERS
                     IRECYIGPHGLRIGEPLAKFHGVLSGIPIYRGESQQLL"
BASE COUNT          370 a          636 c          633 g          403 t
ORIGIN      
        1 ggtgcccgct tcccgctggt cgcggacaat ctcctcgagc agccggcgca aactgagttc
       61 ctccaccttg agggatatct ttccctgggt gacgcgggcg acgtcgagaa gatcgtcgag
      121 caggcggctt aagtccagcg tctgcctgtc gatgatgtcc cggcttttcg caaccgccgg
      181 atcctctatc tcggacatgt tcatcagctg cacggcgttc ctcaccgcgc tcagcgggtt
      241 cctgagttca tggctcagtg tcgccaggaa ttcgtcacgg aggcgcagct cggtatgcag
      301 gttgagcatg aggtccctta cctggtactg gcgccgccgc gccgccagcg ccgatcggag
      361 ggtgctcgcc aggctgacga cgaccacggg ccgcgacagg aaagtgacct tgtaaccttc
      421 aaggcgtcgc aggatgtgtt cgcgcttgta cagttcgagg tcgatgtttg cagcgatgac
      481 gatcggtatc gaggaccacg agggctggtc gtcgaagcgg tcgatcaggg agacgagggc
      541 ggggtcccat agtgcctcct ccgtgatgag cacggcgccc acgccctctt cgagttcggc
      601 acaaagcgag acaatgctgt cgcacacttg cgcccttatc tccagctcgg ccaggacccg
      661 ctgcgcgagt tcaccgtccg caccgaaggg cgcaaaaacc agcacgcgtt cttcgttcgg
      721 cgtcagcgcg gagggaatca tgccggtgtc aaagaagctg ttggctttcg ccgcggtaaa
      781 tcgggatccc ggacagtacg ccgtggaact tcgcgagcgg ctccccgatg cgcaggccgt
      841 gcggccctat atagcactcc cggatgctgc gttcgtgctt gccgaggcgc ttcttgacga
      901 cagagatagc cttgtgcact tcgccgttcg cctcgaagaa tctcatcagc aggattgtat
      961 cgatcaggta gctcacgtcg ataggcgact ccagcgcaga ccccaggatt ccatgctggg
     1021 taacgatcag gatcgacagc acgttgcgcg aattcaggta cttcaggagt tcgaacaggt
     1081 gaacttccat gaagcgcgac tgtggcatca cctgcatgta gccgctcaag ctatcgatga
     1141 cgacgatgcg ggtttcgttt tgttcgactg actccctgac gatgtgacag aactcgccgg
     1201 gggagacatc ggtcgggtcc acgctgcacg tccgtatcaa tccctgctcc acgtattgca
     1261 tgaccggcat gttcatccct tcccaccgca ggcggcacga acggtccgtc tcgtcgaaca
     1321 ggaacatcga cgtcgagtcc ccccgttcag ccgccgcgag ggcgaattgt gcggcaaccg
     1381 tggatttgcc tgtgcctgcc gacccgatca gcatcgccga ggagccgctt tccagtcctc
     1441 cgccgcatag cagatcgagc tcgtcggaac cgctggagat cagtggcgtg tccaccggcc
     1501 cgacagaagc gtgcggcacg agccgcgggt agaccatgag cccgccggtg gtgatcttgg
     1561 cgtcgtgccg gccgctcacg tagcgcatgc cccgcagctt gatgatcgag atacaccttc
     1621 gcgtggcacc gtagagggtg tcgctgtact cgagttcgac gatgccgtga gcaaacgtct
     1681 cgaggcttcc ggagtgttcg ttacctcgct ggagattgtt cagcaggagg accgtgcagt
     1741 gcaggtcggt gagcgtgtcg cgcagcagcc gcatctgtcg cagcaggatc agcggatcgc
     1801 gcgcgagcaa gcggatgtcc gcaagcgagt cgacgacgac gcgcgcgggc cgcgactccc
     1861 tgatttcatc ggtgatcctg gtgatcaggt ccgtcagttc gacctcaccc ggatgaaaca
     1921 gcgtgtattg gtctcgcggc agccccgcgt cgcccgtctg cggcgcgaac agcttgacgc
     1981 cgctcaggtc ccatccatga gactccgcca cctgttgcat ttcatcgtgc gactcggcaa
     2041 gc
//
LOCUS       DASUOM010000632         8202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1180046,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000632 DASUOM010000000
VERSION     DASUOM010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180046"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /locus_tag="VFY27_13190"
     CDS             <1..1814
                     /locus_tag="VFY27_13190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5766514.1"
                     /translation="AAPDKTAIAPAKSIAVLPFVHFGAAGDIDYFADGLTEELINSLA
                     QLPDLKVAARTSSFYFKGRNEDLREIGRKLGVANVLEGSVRRGADSLRVTAQLIQVSD
                     GFHLWSQTFDTGMDDVLAVQGQIARSVSETLKTRLLTDPQESGESTRDARAYHLELVA
                     RSHLRRDELRELETARALYRELLDIETDNASAYTGFAEATISLAQNHLALDFDQARRE
                     SEAAIETALRLAPDSAEAWRVRGLIHRVIGIRSGRHESSGLAEDALRRAVDLDPKNAD
                     ALAMLGVQLLDDGRNEQATALLRRALEIDPLSRRTQQLLGTALADQGRFGEARRQYES
                     LIDLYPDYTYAPIALAYMLIAQGKLDEAVAALDDPTLIAADPEAGLVLAHCYANLGML
                     RESTATLEAIREPPAAAAVAQGAQLLLAREWTALRELADREYATTQDPIWLTARLLEA
                     VLTQDATKAAVALENLGPQITDTGTEVSHSAAIERILAAQGLRLNGRADEAQRIEEAL
                     LRQLDFAPGEYVSIDTLWLRALAYAALGQQDAAVSELTRATDQGFRTLIDFEYFVRLE
                     DYPFMRDVVTDPRYLELATRIEGDNARMREALLAARP"
     gene            1835..2437
                     /locus_tag="VFY27_13195"
     CDS             1835..2437
                     /locus_tag="VFY27_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766515.1"
                     /translation="MRNAPLFFAVLGSLGLAVTACNSPTDGPADSVADAANYDDDTDR
                     SGEENLSPEEQAELMRKAAIEAGIPTSDIEVPVVSARTYVSGQMEIDVSGHFEIDGVM
                     ELDRVASISDGDYTWLQYGTSGGEAPNATVTIGGGDISVSVAVGRYVATATSTECEME
                     TDVTPTSVTGHFNCPEVAGYNQDDRSMENVAIEVEFDAGT"
     gene            complement(2525..2953)
                     /locus_tag="VFY27_13200"
     CDS             complement(2525..2953)
                     /locus_tag="VFY27_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5766516.1"
                     /translation="MKTVRQILGDKGHEVWSVRPYDTVFDSLRVMADKGVGALLVMDG
                     EKLVGIVTERDYARKVILEGKSSKTLQVSDVMTTRVLCVTPDRNVDECMALMTDNRVR
                     HLPVLDHKRVVGVVSIGDLVKATIGEQQILIDQLQQYISG"
     gene            3530..4753
                     /locus_tag="VFY27_13205"
     CDS             3530..4753
                     /locus_tag="VFY27_13205"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX5766517.1"
                     /translation="MASKPVLANSSVRNALLSVNGLSTSLPHDLLPAVTATSGPKWVH
                     QALLGLTHVQVDAIATVERSHHHILFTRNHRYRRSDLRKALEMDRTAFENWTHSAAVL
                     PIDAYPYWKHYFRRFRNGETHPGYRRYFSQVTASMRSRVLKAIRERGPLTPRQVASKK
                     VQWTDPYFARPSLAKMTMEYLWRAGTLAVARRNGQEKVYDLAQRIIPEHHFSRTVTRA
                     QFIDWACVEALKRLVTATPTQISRFFHAVSLEEARTWCEKNRGKGVQAVAMTCADDQV
                     IRGLYGLTPVVDRLDAFPDPPSGLHVLNPFDPLVHDRGSRRTSSIFGFEQRMEIWVPP
                     EKRKFGYYVLPILEGSRFTGRVDAKLDRSEGIFRVLGLWWERGVGRSSARLARLHREL
                     VELARFVGVERVTGL"
     gene            complement(5144..5335)
                     /locus_tag="VFY27_13210"
     CDS             complement(5144..5335)
                     /locus_tag="VFY27_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766518.1"
                     /translation="MPITGRLSGVPLLKPALARLDGSIPGSAERLAVLDHHYGAGPVT
                     ARVQVSGDDTGNTVSFSDG"
     gene            complement(5382..7634)
                     /locus_tag="VFY27_13215"
     CDS             complement(5382..7634)
                     /locus_tag="VFY27_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766519.1"
                     /translation="MANDIDNLDGLIDAIAERMLGKLTGRLNLDPAGAPLPQLPDWPF
                     GRKNVEPLIVHPRLRVQGLELTQSTQYFGTGYGAENSVPIVALKPLVVRAYPYVSAGL
                     VEGDTLSDKTVTGELVLLRQGKEVYRTGPTRPAGARVGPVTRIDRDLWDEETTFYTSS
                     GKLAVELVSVKWNPTLNFFVPAWYVRAGQTTAVVYLWLTSGEGGTAAASESVQVISVN
                     APKLALVRVNWKNPADDVITSPSDADLLGLCALATRMLPFPYFETTVLGVEETKSGDF
                     SGPPLKADGTPNPGGCNTAWVDLLTGLKVTRIFSALFGIAEIVYGVVPQVGALTTTNP
                     RNTGCGWGDDGVGGCLVGDELAFAHEVGHLHTCGHIGDPTLAGYDASYPNYGGSKSLI
                     GEVGIDTALGAAPLSDPRTVHDLMSYKFPQWVSPYTYLKILDNRDKHLSAAADPRKVR
                     PLLIIIFTVDRLADGSRKIDKLRAHVVQGPGFVPRPRGPAPGNWFIELIDDHERIIAS
                     HACRISIAHGSGCRCCGGETDLERAPHLDFVEAVEWNDAVTSLQITHGETQIVRLDVG
                     EAPHIEIGGPQRSDESLILDIRVHHPRTEAALSVLYTADDGETWSPVAIDPPQDKPVA
                     IAVSKLRGGESCRFRAVATAEFRSASAESESFSLPRTDRALLIEMKESQCKAGRVDLA
                     AMIDLRGHAGVEPHEVVWHSDLAGELGRGYSLSVELEEGRHRISATIPGGANDRVQKA
                     GIIVVGGKGR"
     gene            7886..>8202
                     /locus_tag="VFY27_13220"
     CDS             7886..>8202
                     /locus_tag="VFY27_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766520.1"
                     /translation="MKILLSAGVAAASMYYLDPSRGNERRADARARLEHGKDKLSREA
                     GKLDHGARNLAAGAAGTFASFLSRNRSHGKARAALRNAPRAKVLLIPGTWIVTVGLGA
                     ATM"
BASE COUNT         1569 a         2617 c         2517 g         1499 t
ORIGIN      
        1 acgccgctcc cgacaaaacc gcgatcgcgc cggcgaagtc gatcgcagtg ctgccattcg
       61 tgcatttcgg cgccgccggc gacatcgatt actttgccga cgggctcact gaggaactca
      121 tcaacagcct ggcacagctg cccgatctca aagtcgcggc gcgcacgtcg tcgttctact
      181 tcaagggccg gaacgaggat ctgcgcgaga taggccgcaa gctcggcgtg gccaacgtgc
      241 tcgaaggcag cgtccgccga ggggccgaca gcctgcgcgt taccgcacag ctcatccagg
      301 tttcggatgg gtttcatctc tggtcgcaga cattcgatac cggcatggac gatgtgctgg
      361 cggtccaggg gcagatcgcc cgttcggtat cggagacgct caagacacgt ttgctgaccg
      421 acccgcaaga gtcgggcgag tcgacacgag acgcacgtgc atatcacctc gagctcgttg
      481 cgcgctcgca cttgcgccgg gacgagttac gtgaactcga gaccgcgcgc gctctctatc
      541 gggaactcct ggatatcgag actgacaatg caagcgcata taccggtttc gccgaagcta
      601 cgatctcgct tgcacagaat caccttgcgc ttgatttcga ccaagctcgt cgcgagagcg
      661 aggccgcgat tgaaacagca ctcaggcttg cgccggactc agcggaagcg tggcgcgtac
      721 gcggcctgat ccatcgcgtt atcgggattc gttccggacg gcatgagagc tccggtctcg
      781 cagaagatgc gttgcggcgt gcagtggatc tcgacccgaa gaatgccgat gccctcgcaa
      841 tgctcggcgt gcaattattg gacgatggcc ggaacgagca ggctaccgcg cttctgcggc
      901 gggcactgga gatcgaccct ctgtcgcgcc gcacacaaca gctgctcggc acggccctgg
      961 cggaccaggg caggtttggc gaggcgcgtc gccagtacga gagcctgatc gacctctatc
     1021 ccgattacac ctacgcgccg atcgccctgg cttacatgct catcgcgcag ggcaagctcg
     1081 acgaggccgt cgccgccctg gacgatccaa cgttgatcgc cgccgatcct gaagctgggc
     1141 tggtcctcgc ccactgctat gccaatcttg gcatgctgcg agagagcaca gccacactcg
     1201 aagccattcg ggagccgccg gccgccgccg ccgttgcgca gggtgcgcag ctgctccttg
     1261 cacgcgagtg gaccgcgctt cgcgaactcg ctgaccgcga gtacgcgaca acgcaagacc
     1321 cgatttggct cacggcgcgt ctgctggagg ccgtgctcac ccaggacgcc acaaaagctg
     1381 ccgtcgcgct cgaaaatctg ggaccccaga tcacagacac cggaaccgaa gtgtcgcact
     1441 ccgccgcaat cgagcgcatc ctggccgcgc aaggactgcg actgaacgga cgagccgatg
     1501 aggcgcaacg gatcgaggaa gctctgcttc gccagctcga cttcgctccc ggcgagtatg
     1561 tatcgatcga tacgctctgg ttacgtgcgc ttgcgtacgc ggcgctcggc cagcaggacg
     1621 ccgccgtttc ggagctaacg cgcgcgaccg accagggttt ccgcacgctc attgacttcg
     1681 agtacttcgt gcggctcgag gactatcctt tcatgcgcga cgtcgtaacc gatccgcgct
     1741 acctggaatt ggcgacccgc atcgagggcg acaacgcgcg catgcgcgaa gccctgcttg
     1801 cggcccgtcc gtaaacaacc gaggtaacgt aaccatgcgc aacgcacccc ttttctttgc
     1861 cgtgctcggc agcctcggcc tcgccgtcac cgcgtgcaac tccccgaccg acgggcccgc
     1921 cgactccgtg gccgacgccg caaactacga cgacgacacc gacagatccg gtgaggagaa
     1981 cctgtcgccg gaggagcagg ccgagttgat gaggaaggcg gcgatcgagg cgggaatccc
     2041 gacttccgac atcgaggttc cagtcgtcag cgcgcgaacc tacgtctccg ggcaaatgga
     2101 gatcgacgtc agcggccact tcgagatcga cggcgtgatg gaactcgacc gcgtggcgag
     2161 catctccgac ggcgactaca cctggctcca gtatggcacc agcggaggcg aagcgccgaa
     2221 tgcgaccgta acgatcggcg gcggcgacat cagcgtttca gtggccgtcg gccgctacgt
     2281 tgcgaccgcc acgagcacgg aatgcgagat ggagactgat gtgacgccca cgtccgtcac
     2341 gggtcacttc aactgcccgg aggtggccgg ctataaccag gacgatcgca gcatggaaaa
     2401 cgtggcgata gaggtcgaat tcgacgcagg cacgtgacgg accaccggac ccggaccatt
     2461 gcggcgatcg gccggcgact gcctggcaga ccgcactgct acggtgtgcc gacctgcgcc
     2521 gtagctagcc cgaaatgtat tgttgcagtt gatcgatcag gatttgttgt tcgccaatgg
     2581 tcgccttgac caggtcaccg atcgagacaa ctccgactac tcgcttgtgg tcgagaacgg
     2641 ggagatggcg gacgcgattg tcggtcataa gggccataca ttcgtcaaca ttcctgtccg
     2701 gtgtgacgca caggacccgc gtcgtcatga cgtcactgac ctgcaaggtc ttcgaagact
     2761 tgccctcgag gatgactttt cgggcgtagt ctcgctcggt cacgattccg accagtttct
     2821 cgccgtccat caccagcaga gcgccgacgc ccttgtccgc catcacccga agtgaatcga
     2881 aaaccgtgtc gtacggccgg accgaccaaa cttcgtgccc tttgtcacca agtatctgcc
     2941 tgacagtttt catttcagct ctctccggtt tttgtccaga atagtcgata cacgccgttc
     3001 ctgcattacc ggtatcgctg cgtcacggtt gtgtgagcgg atcggctatc cccaactact
     3061 ggaagcaggg caatctctgc tgcatccccg gccagagatc gtagtccgcc tgcggttcca
     3121 gccagctctc ggaggtcgga ccgcgctttg ggcctcgtgg ccaggcctcc agccgcctgg
     3181 ccataccggg aaatactgga cggacaatga gcttccagcc cggcccattc atgtccaggg
     3241 gacgccgcca acccgccgga gctaccggga gagacggggc aagtcctgcc tatttcgtcc
     3301 gcctgtattc acaggacggc gacgtgttat gccattggcg ccggcggcaa aaatgctgat
     3361 cgacctccgc tgcagtttcg actaaggtac cgaagacgcg gcagaacgga gcggtcgtgg
     3421 agcggccgac aggttgcgag tagccaaggc ggtcagaaaa cgtctggtca aggctatcgg
     3481 gagctggtct atttcgcggc cggttcccgc cgcaatgaaa gggagatcga tggcgtccaa
     3541 gccggtgtta gcgaactcca gcgtgcgcaa cgcgctcctc agcgtgaacg gcctttccac
     3601 gtcccttccg cacgaccttc tgccggcggt gaccgccacg tccggcccca agtgggtgca
     3661 ccaggcgcta ttgggactca cccacgttca ggtcgacgcc atcgcaaccg tagagcggtc
     3721 gcaccatcac atcctgttca cccgcaacca ccggtaccgc cggtctgatc tgagaaaggc
     3781 tctggagatg gaccggacgg ctttcgagaa ttggacccat agcgccgcgg tgctgccgat
     3841 cgacgcctat ccctattgga agcactactt ccggcgcttc cggaatggcg agacgcatcc
     3901 cggatatcgc cggtacttct cgcaggtgac cgcctccatg aggtcgcggg tgcttaaagc
     3961 catccgggaa cgcggaccct tgacaccgcg ccaagtggcc tcgaagaagg tccaatggac
     4021 cgatccgtac ttcgcgcggc cgtctctcgc gaagatgaca atggagtacc tgtggcgggc
     4081 tggcacgcta gccgtcgcga ggaggaatgg acaggagaag gtctacgatc tggcccaacg
     4141 catcattccc gagcaccact tctcccgcac agtgaccaga gcacagttca tcgactgggc
     4201 ctgcgtcgaa gcgctgaagc gactggtcac cgccacgccg acacagatct cgcgcttctt
     4261 ccacgcggtc tctctcgagg aggcgcgcac gtggtgcgag aagaaccgcg ggaagggcgt
     4321 acaagccgtc gcgatgacgt gcgcggatga tcaggtcatc cgcgggctct acgggctcac
     4381 gcctgtggtc gatcgtctcg atgcgtttcc ggatccgcct tccgggcttc atgtcctgaa
     4441 cccgttcgat cccctggtcc acgaccgcgg ctcccggcgc acgtccagta tctttgggtt
     4501 cgagcagcgc atggagatct gggtgccgcc ggagaagcgc aaattcggct actacgttct
     4561 gccgatcctg gaagggagca ggttcaccgg gcgcgtggac gcgaagctcg atcgatccga
     4621 aggcatcttt cgcgtcctgg gcttgtggtg ggagcgtggc gtcggacgat cgtcagcccg
     4681 cctcgcccgg ctgcaccggg agctcgtgga gctggcccgc tttgtaggtg tggagcgtgt
     4741 caccggtctt taggacggcc tgacgtgcca cggcctcgac cgacagaagg gacgtgcact
     4801 tcgtgtatcg caaacgagcc agcaacgagc tgacccgaat actcgagccg tacagactgc
     4861 cgcagcttgg cgattcgcgt gcagaagccg gtttcatcca ggggcctgcg tcttgacgaa
     4921 ttcaccggat tgggctgcac ggcaagcatc cgtcccctgc ccgattacca tagcgccgcg
     4981 ttgggattgc ggaaatgaat gcggtcacac ttctctagtg actgtgaaca cagcggcgtc
     5041 gtcgggaaac tcaagttcag cctcgcgacc gagttcgcag ttcgctcgcg ggagctctac
     5101 cgtcgagaac cacgactggt caggggattg ataacttgct aatttatccg tcgctgaaag
     5161 aaacggtatt gccggtatcg tcgcccgaca cctgcacccg ggccgtcacg ggaccggcgc
     5221 cgtagtgatg gtcgagcacg gcaagtcgtt ccgcggatcc gggaatcgag ccatcgagcc
     5281 gggccagtgc aggcttcagg agcggcaccc cggaaagccg cccggtgatg ggcacccgat
     5341 cgctgtcaca gctgagaaag cccgcaacct tgcggcaggt ctcaccgccc tttcccgccc
     5401 acgacgatga tgccggcttt ctgcacgcgg tcgttggcgc caccaggaat tgttgccgaa
     5461 atccggtggc ggccttcctc gagctcgacg ctgagcgagt agccgcgccc gagttcaccg
     5521 gccagatcgg aatgccacac aacctcgtgc ggctcgacgc cggcatggcc gcgcaggtcg
     5581 atcattgccg cgagatcgac acggcccgct ttgcactgcg actctttcat ttcgatcagc
     5641 agcgcgcggt cggtgcgcgg cagcgagaag gattcactct cagccgatgc cgaccggaat
     5701 tctgccgttg ccaccgcgcg gaaacggcat gactccccac cgcgaagctt ggagaccgcg
     5761 atcgctacgg gtttgtcttg cggcgggtcg atcgcgaccg gagaccatgt ctcgccatcg
     5821 tcagcggtat acagaacgct gagtgcggcc tcggtgcgcg gatgatgcac gcggatgtcg
     5881 aggatgagcg actcgtcgct gcgctgcggt ccgccgattt cgatatgcgg cgcttcgcca
     5941 acatcgaggc gaacgatctg agtctcgccg tgcgtaatct gcaagctcgt caccgcgtcg
     6001 ttccattcca ccgcttcgac aaaatccaga tgaggcgcac gttcgaggtc ggtctcgccg
     6061 ccacagcagc ggcatccgct accgtgtgcg atcgagatgc ggcaggcgtg gctggcgatg
     6121 atacgctcat gatcgtcgat gagttcgata aaccagttgc ccggtgccgg tccccgcggc
     6181 cgcggcacaa agcccggacc ctggacgaca tgcgccctga gcttatcgat cttccgcgag
     6241 ccatcggcca gccggtcaac ggtgaagatg atgatcagca gcggccgcac tttgcgcgga
     6301 tcggcggcgg cgctcaggtg cttgtcgcga ttgtcgagga ttttcaggta ggtgtacggc
     6361 gacacccatt gcggaaactt gtacgacatc aggtcgtgca cagtgcgcgg atcgctgagt
     6421 ggcgccgcgc cgagcgccgt atcgatacct acctcgccta tcagcgactt gctgccacca
     6481 tagttcgggt agctggcatc gtacccggcc agcgtcgggt cgccgatatg gccgcaggta
     6541 tgaagatgcc caacctcatg ggcgaaggcg agttcgtcgc cgaccaggca tccgcccaca
     6601 ccgtcgtcgc cccagccgca accggtattg cgcgggttcg tggtggtcaa cgcgccaacc
     6661 tggggcacaa cgccatacac gatctcggca atgccgaaca gtgcggagaa aatgcgcgtg
     6721 accttgagac cggtgagtaa atcaacccag gcggtattgc agccgcccgg gttgggcgtg
     6781 ccgtcggcct tgagcggcgg gcccgagaaa tcgccggact tggtttcttc gacgcccagg
     6841 accgtcgtct cgaaataagg aaacggcagc attcgcgtcg ccagcgcaca caggccgagc
     6901 aggtccgcat cgcttggcga ggtgatgaca tcgtcggctg gattcttcca gttgacacgc
     6961 accagcgcga gcttcggcgc attcactgag attacctgca cgctttccga ggctgctgcc
     7021 gtgccgcctt cgccactggt caaccaaagg tacaccaccg ccgtggtttg tccggcgcgc
     7081 acataccacg ccggtacgaa aaagttcaag gtcgggttcc atttgacaga gacgagttcg
     7141 acagccagct tgccggacga ggtatagaac gtggtttcct catcccagag atctcggtcg
     7201 atgcgcgtca ccggtcccac gcgtgcgccg gcggggcgcg tcgggccagt gcgatagacc
     7261 tctttgccct ggcgcaggag caccagctca ccggtgacag tcttgtccga cagcgtatcg
     7321 ccttcgacca gcccggcgct cacatacgga taagcgcgca caaccagggg tttcagcgcg
     7381 acgatgggca cggaattctc cgcgccgtag cccgtgccaa aatactgcgt ggattgcgtg
     7441 agttccagac cttgaacgcg aagcctggga tgtacgatca acggttccac atttttgcgt
     7501 ccaaacggcc agtcggggag ttgcggcagc ggtgcacccg cagggtccaa attgagtcgt
     7561 ccggtcaatt tgccgagcat gcgctccgcg atcgcatcga taaggccatc caggttgtca
     7621 atatcgtttg ccatgacaga tctccctgag acctcggtcc gtgggttacg ggctagcatc
     7681 ttcctctcgt aattgatcgg cgacaagtaa ccctacccgt gcaattaaac gcacagcgtg
     7741 tcagctcgca gtacgttgcg acccgtgctg gcacgactct tgctagctac tacacagatc
     7801 aggcacgcac tagcgcaaag caggattcga agccttgcgc cgcgtcaatc cactttctgt
     7861 gagcaagtaa actatgcaaa cgttaatgaa gattctactg agtgccggcg ttgctgcggc
     7921 atcgatgtat tacctggacc cctcacgggg caacgagcgc cgcgccgatg cccgcgcccg
     7981 gctcgaacac ggtaaggata agcttagccg ggaagccggt aagctggatc atggtgcccg
     8041 caatctcgcc gcgggcgccg ccgggacgtt tgcatcgttc ctgagccgca accggtccca
     8101 cggcaaagcg cgcgcagccc tgcggaatgc gccgcgtgcg aaggtcctgc tgatcccggg
     8161 cacctggatc gtcaccgtcg gtctgggtgc ggccacgatg ta
//
LOCUS       DASUOM010000633        17431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1664882,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000633 DASUOM010000000
VERSION     DASUOM010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 17431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17431
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664882"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFY27_13225"
     CDS             <1..996
                     /locus_tag="VFY27_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766521.1"
                     /translation="ENRVGELLPDVAAGEPLLEQFVDVARAHMGAGETEWREQIVLRG
                     EVGRRVLTCACTTLPGDDDHAPGFVVVFDDITALLQAQRDAAWGEVARRLAHEIKNPL
                     TPIQLSAERLRRKYLHTMSRDEAQILDRATHTIVQQVEAMKEMVNAFSNYARAPDIDI
                     SAFDLEKLAHEVVDLYRAQESGIEIELEADRDLPRIEADIGRIRQILHNLIRNSIEAL
                     ESSTHGHILVSARKADLNGIEVVEIEVKDNGPGFQTGSVSQVFDPYVTTKPRGTGLGL
                     AIVKKLVEEHAGTIDAENREEGGALIRIQLPVNEAAREAMTVLLPRRAERRRERA"
     gene            993..2363
                     /locus_tag="VFY27_13230"
     CDS             993..2363
                     /locus_tag="VFY27_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5766522.1"
                     /translation="MSTAHVLVVDDEADIRALIQEILTDENYQVTVAANAAEARSARK
                     ENRFDLVLLDIWMPDTDGISLLREWSEHGELETPVVIMSGHGTVDTAVEATRLGALDF
                     VEKPLSIAKLLRTVERGLESARKQAGSGRGMLPSLLAPVGRSKLMQDLRDKVQQYANH
                     DSPVLLIGEPGTGRGAFARYIHALSPRAEEPLEVLLAASLTETNTEEQLFGVEKNGEV
                     RPGFFERAGNGTLLIEELSDLNDQAQKLLLSALEQRWFTRVGGHEQVELCSRILASVR
                     DDYESAIERGQLRRELVSMLNVLSVRVPPLREYAEDVPELLHYYVDKLVDSEGLAFRR
                     FSVAAQNRLRNYPWPDNVRELKNLVRRLLLTGSGEEIALDEIERELSASSQSDEPLVK
                     QDLLSLPLREAREQFERAYLQQQLVLCDGKVGKLAKRVGMERTHLYRKLRSLGVDFRA
                     ISTADD"
     gene            complement(2371..3534)
                     /locus_tag="VFY27_13235"
     CDS             complement(2371..3534)
                     /locus_tag="VFY27_13235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5766523.1"
                     /translation="MFAKARRPLAISAIVALTLGGCAAGPKPSAEQRVKTGAPQQVSR
                     QAPPTPSAARELTIAAVGDIMLGTDYPENHLPDDDGAGFLAGVAPVLSAADLTFGNLE
                     GVLVDGGTPRKVCTNPSACFLFRTPSRYVRHFVDAGFDVMSLANNHSRDFGEGGRSAT
                     MRTLAAAGIAHSGREGDFASIDVKGLKIAVLAFAVTQKSNLLHDYDAAAEIVLAHDAS
                     HDIVIVSFHGGAEGNDVTRLPFAEEEYYGEPRGDVVYFARKMVDAGADLVLGHGPHVV
                     RAMERYQDRIIAYSLGNFATYYGISVNGLNGVAPILQVTVDGTGRFIEGQIYSTVQVR
                     PGGPHRDESRHALHLIRDLSRQDFLTPGIRFLDNGRILPEARSTFREHGHVPE"
     gene            complement(3534..4112)
                     /locus_tag="VFY27_13240"
     CDS             complement(3534..4112)
                     /locus_tag="VFY27_13240"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5766524.1"
                     /translation="MAKRRSRHRAESSEYPGWAWMLFGLLVGLSVAFAIYINGRNSRI
                     PAEPQAREAASMEQATDQAAAPPVEIPSGAADEPAKPRFDFYSMLPNFEVIIPEQEPD
                     VKTDTAVQAVVEPGVYVLQAGSFTQHADADRRRAQLALQGIESTIQQVTIDDKTYHRV
                     RIGPMEDLEQLNVVRSRLREADIDVLRIRLGD"
     gene            complement(4116..5867)
                     /gene="argS"
                     /locus_tag="VFY27_13245"
     CDS             complement(4116..5867)
                     /gene="argS"
                     /locus_tag="VFY27_13245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5766525.1"
                     /translation="MKSSIAELVDRAIAGLPELADSPEVTSTRTTVERTRDPRHGDFS
                     TNIAMRLAKAVGSKPRELAGQIIALLPASDLVEKVEIAGPGFINFHLSETALQQEVAA
                     VLREGSAYGRQATRAEPRILLEFVSANPTGPLHVGHGRHAAYGATLGNLLEAAGYSVG
                     REYYVNDEGRQMDILGASVWLRMLERDGEIVAFPRAGYRGDYIKEIAEAIDAPGLPKV
                     KAAELKQRLPADEPEGDKEAYITAVIAAATELLGAERFISIRRQALDSILDDIRDDLG
                     EFGVEFDRWFSERSLSDDRRIEEALAVLKKRQMLYEKEGATWFRATDFGDEKDRVVVR
                     ENGRTTYFASDIAYHYDKRQRGFDHLLDILGADHHGYVARVRAGLEAMGFAGDSLEVE
                     LVQFVTLYRAGEKLAMSTRSGEFVTLRQLRDEVGKDAARFFYVMRSHDQHLDFDLELA
                     KSRSNDNPVYYIQYAHARVASVFRQLAEKGLAWDEQDGLSNLPRLEEKHEKSLMNSVS
                     RYPEIIEAAAANRAPHHLVQYLRELANEFHTYYNAHTFIVDDDDLRNARLALIAATRQ
                     VIASGLDILGVSAPESM"
     gene            complement(5926..8130)
                     /locus_tag="VFY27_13250"
     CDS             complement(5926..8130)
                     /locus_tag="VFY27_13250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5766526.1"
                     /translation="MSREPVFRVAINAPLSRLFDYLPPKAGNDSLQPGSRLRVPFGRS
                     TQTGMLLEVAGHSEIARTKLRRAHEIIDEEPLLRPSDLWLIRFTSEYYHHPVGEVVAA
                     ALPALLRQGKPLHPTIRHLSLTDAGRQADATAVAKKAPKQAELLSLLQQAGCLNFESL
                     GQLAPGWKRLVKSFREKGWVEVTASRGEATASADSADAEGGPPLNQDQAAAFAAISGR
                     TRFGVSLLDGVTGSGKTEVYLRLIEETLARGKQALVLVPEIGLTPQFVARLKKRSGVL
                     PVILHSALSDTQRLDAWRAARDGSARLVVGTRSAIFVPLNEAGLIVVDEEHDSSFKQQ
                     EGLRYSARDLAVARAKELDIPVILGSATPSLETLQRCAEGSYERLVLPVRAGNAVPPL
                     LRLVDLNRHQPLNGLSRPVIEAMRRHSAEGGQVLVFLNRRGFAPTLICEHCGAIAECS
                     RCDARMTVHARSLRLICHHCGAQRALDPACRECGGTVKPLGHGTERLEDALQLHFPDA
                     LVSRIDSDSTRRSGALDKALEMATTGETEILIGTQILSKGHHFPNLTLVVVIDADQGL
                     FSTDFRGTERLAQGLMQVAGRAGREQRQGEVMIQTSFASHPFWHELFQGGYERVAASA
                     LAERENAAWPPFSRLALLRAAATRRQDTHDFLARSRRTAEAAKVEGVRILGPVSAPME
                     RRAGRYRAQLLFQSRHRQPLHRLLQAVRPQLEAGPWARKVRWSIDVDPVELF"
     gene            complement(8147..9508)
                     /locus_tag="VFY27_13255"
     CDS             complement(8147..9508)
                     /locus_tag="VFY27_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5766527.1"
                     /translation="MRWLIEASYFVAAILFIYGLKRMSSPVTARGGIVWAGAGMLVAT
                     LVTFLWPGMDNYVLMIVAIALGGTLAWVSGKRVAMTDMPQMIALYNGMGGGAAAAIAA
                     VELFGGEDHGLTFGVLAVLGGLIGSVSFSGSLIAFAKLQGLMKSTVRFGGQQVFNLVL
                     LAVLLVLATVIVTQHGGPALIAIFFLLALVLGVTMTLPIGGADMPVVISLYNALTGLA
                     VAFEGFVLQNAAMIIAGTVVGAAGTLLTQLMAKAMNRSLGNVLFSGFGDTAAVASEVH
                     GSMKPIEASDAGVMMAFANKVLIVPGYGMAVAQAQHKVWELAQILIERGVEVKFAIHP
                     VAGRMPGHMNVLLAEAGVPYDVIYDENEINAEFATADVALVIGANDVVNPVARTDPAS
                     PIYGMPILNADKARNVIVIKRGQGAGFSGIENGLFFLDQTRMLYGDGQAMASELIQAV
                     KSL"
     gene            complement(9526..9816)
                     /locus_tag="VFY27_13260"
     CDS             complement(9526..9816)
                     /locus_tag="VFY27_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5766528.1"
                     /translation="MIDGFIALYIFMLAAFVGHEVIGRVPVILHTPLMSGSNFVHGIV
                     LVGAMVALGRAETGLEMTIGFIGVFLAAGNAVGGYVVTERMLEMFKSSKEKR"
     gene            complement(9863..10993)
                     /locus_tag="VFY27_13265"
     CDS             complement(9863..10993)
                     /locus_tag="VFY27_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX5766529.1"
                     /translation="MPLTVGVLKETLEGETRVALTPEISTKLKKLGVSVVMERDAGAG
                     SQLLDSAYEGVEFTDAVGVTSKCGLLLTVQPPSAETVRELGEGSVTAGFVYGHLHADL
                     VDVLKEKRHTAFAMELVPRISRAQTMDVLSSQAAAAGYQAALIAASTLDKFFPMLTTA
                     AGTIRPAQVLVIGAGVAGLQAIATAKRLGAVVKGYDVRGATKEQVQSLGAKFVETGVS
                     AEGAGGYARELTEEEKQKQQAALEKEIAVSDVVISTAAIPGRPAPRIISAAAVRTMMP
                     GAVIVDLAAETGGNCELTRAGETVLENKVKIVGPVNLPAKLGRHASEMYARNLFNFLS
                     PAISEGELQIDWEDEVFARTVLTRDGEIRHEGTRQSLEAKNQ"
     gene            11245..11886
                     /locus_tag="VFY27_13270"
     CDS             11245..11886
                     /locus_tag="VFY27_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5766530.1"
                     /translation="MRFGAASDISQQVLRTDVAGMPLEWIDYREAVRLYHTEQVAYAC
                     GTRLYTIFGGYSALSGRRSAIDVSSIVATRGTSQGLAYVRDQYVPPLNNRTLFKRDAN
                     LCLYCATRFPTRDLTRDHITPLSQGGRDAWNNVATACKRCNNHKGGRTPEQSAMQLVA
                     VPFTPTYAEYIYLKGRKVLADQMKYLLAHIPRSSPLHARLSDHLSDGSLFLDS"
     gene            11975..12901
                     /locus_tag="VFY27_13275"
     CDS             11975..12901
                     /locus_tag="VFY27_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5766531.1"
                     /translation="MQYLIDASVYVFRAHHSLPDDMVDGDGNPVNALYGFCRFIGDFI
                     EQVRPEYLAVLFDESLEASFRSEIYPAYKANRDPAPVELKRQFIQCREFTRALGIMEC
                     AHPAFEADDLIGTLVEAGRRAGRASTIVSRDKDLAQLVSEQDVFWDFAGKGKLTYDQI
                     PGVFGVWPEQIADFLALAGDSVDNIPGVPGVGKKTAAALLKHFGSLDGIYANLDRVHE
                     VCCRGARTLALKLATHREAAMLARRLTGIACEVPLERALSCLRPVAPDLGAINALFDR
                     ASIGMALRRQAERLSDLVAEPNLPRLNLQAAY"
     gene            12948..13817
                     /locus_tag="VFY27_13280"
     CDS             12948..13817
                     /locus_tag="VFY27_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5766532.1"
                     /translation="MSDWHDLKQALRKQGIAIADAKPRAVGGGDIGSAWKVPTDREPV
                     FLKTGPREFLEIFSAEAEGLEELGSANAVRVPAVLAFGLAGSDAYIALEWLDLGGRDT
                     AAAILLGRQLALQHRNLDERFGWHRDNMIGTTPQLNDRDDDWIRFFRERRLLVQLQLA
                     ESNGFGGELLQEGARLARNLDLLFDGYAPVPSLLHGDLWGGNWSSVAGEPVIFDPAVY
                     YGDRESDLAMTRLFGGFGSGFYRAYEDEWPLARGHEKRVALYQLYHVLNHLNMFGVAY
                     LSRAMSLIRELQR"
     gene            complement(13798..14742)
                     /locus_tag="VFY27_13285"
     CDS             complement(13798..14742)
                     /locus_tag="VFY27_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5766533.1"
                     /translation="MVGSQGFVPDPRNEQVKIWLNGELVLRSEAKVSVFDAGFVLGDG
                     VWEGLRLVNGRLAFLDEHLDRLYSAAKVLAIDIGMDRKEMARALYRTVESNEMRDGVH
                     VRLMVTRGIKITPNQDPRAAISGPTIVIIAEHKEPHPESRERGLVLVTSPYRTSTPDV
                     FDMHLNTHSRLNLIQSLIQAAALGADEALMLDPYGHVASCNATNFFIVRGGAVWTSTG
                     RFCFKGITRGHVVELCRQHGIPVEERDFTLADAYSADEAFVTGTFGGVTPVATLDGRE
                     FAAGASKSVTTKLSGLYRDLLRHECAAGPEPDFSAVTP"
     gene            complement(14742..15503)
                     /locus_tag="VFY27_13290"
     CDS             complement(14742..15503)
                     /locus_tag="VFY27_13290"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766534.1"
                     /translation="MSQPITGDDSVRIAMWSGPRNLSTAMMRAWENRPDTAVVDEPFY
                     ACYLKFSGAKHPFRDEVLAGQSSDWHAVARKLTAETPGGRRIFYQKHMTHHMLPEAPL
                     EWLERVSHAFLIRHPQEVSASWAHALKSVPSLDELGYVRQVELFDRIADTRGEAPPVF
                     DSRDVLERPEPALRALCNALRVPFDERMLSWPEGPRETDGVWAPHWYQSVWRSSGFGP
                     ARRQAQPAPELLPVIDAALPFYERLAQHRIPVEGQ"
     gene            complement(15611..16462)
                     /locus_tag="VFY27_13295"
     CDS             complement(15611..16462)
                     /locus_tag="VFY27_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766535.1"
                     /translation="MAQEKSPAETLNPQKPAAQVESLNARRQPQDHRALPSRRQVASE
                     DLDLIVRRAAELQNKRGNTASQLLTEEEVVEIGRQVGLEPAHVRRAMAEVHAESLAPE
                     RPQGNRILDLVAGDARVEVRRVVAGEPTLIQQQVEMLLREREKLSALRRRPARSVWEA
                     SAGIMDRLDRFMNFSGKEYALADTRQVELVIAETEPGWSLVTLAADLANKREEALYTA
                     GSCVGVAAILAAAFTAMDAGYGVFLPVVVGLASAAVAIPCLRWSVGKRHERIAVILEG
                     LIDHVDR"
     gene            complement(16637..>17431)
                     /locus_tag="VFY27_13300"
     CDS             complement(16637..>17431)
                     /locus_tag="VFY27_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5766536.1"
                     /translation="PARKLILDGEVIALRNDRTPHPFQVTMRRFGRTLDVERLRESLP
                     LDAFYFDCLHVDGDDLIDLPAFERFERMSGVVPAAFLIPRLVTADPDAAAGFLEEALA
                     AGHEGLMAKSLDSLYEAGNRGAGWLKIKPAHTLDLVVIAAEWGSGRRRGWLSNLHLAA
                     RDPGADEENGFVMLGKTFKGMTDKMLEWQTKRLLELEAGREGHVVHVRPELVVEIAFN
                     ELQSSSQYPGGMALRFARVKRYREDKRPEDADTIETVREIFARRTS"
BASE COUNT         3124 a         5542 c         5487 g         3278 t
ORIGIN      
        1 gagaaccggg tgggcgagct tcttcccgat gtggcggccg gcgagccgct gctggagcag
       61 ttcgtcgacg tcgcgcgcgc acacatgggt gcgggcgaga ccgaatggcg tgagcagatt
      121 gtccttcgcg gcgaagtcgg ccggcgagta ctgacctgtg cctgcacgac tcttcctggc
      181 gacgacgacc acgctcccgg gtttgtcgtg gtattcgacg acatcacggc gttgctgcag
      241 gcgcagcggg atgcggcgtg gggcgaggtc gcgcgcaggc tggcccacga gatcaagaac
      301 ccgctgaccc cgatccagct ctcggccgag cgcttgcgcc gcaagtacct gcacacgatg
      361 tcccgcgacg aggcgcagat cctcgatcgc gcaacgcaca cgatcgtgca gcaggtcgag
      421 gcaatgaaag aaatggtgaa cgcgttcagc aactatgcgc gagcgccgga tatcgacatc
      481 agtgcgttcg acctcgaaaa gctggcgcac gaggtggtcg atctctatcg cgcgcaggaa
      541 agcggaatcg agatagaact cgaggcagat cgagacctgc cgaggatcga agcggacatc
      601 ggacgcatcc ggcagatcct gcacaatctc attcgcaatt cgatcgaggc actggagtcc
      661 agcacccacg gtcatatcct ggtgtcggcg cgcaaagcgg acctgaacgg gattgaagtc
      721 gtggagatcg aggtcaaaga caatggaccc ggtttccaga ccggatccgt aagccaggta
      781 ttcgacccat acgtaacgac gaagccgcgc ggcaccggcc tgggtctcgc cattgtaaag
      841 aagctggttg aggaacatgc cggcaccatc gacgcggaga accgcgagga aggcggcgca
      901 ttaattcgga ttcaactgcc ggtaaatgaa gcggcacgag aagccatgac cgttctgttg
      961 ccgagacgcg cagaaagacg gagggaaaga gcatgagcac agcacacgtg ctggttgtgg
     1021 acgatgaggc cgacatcagg gcactgatcc aggaaattct cacggacgaa aactaccagg
     1081 tgacggtcgc cgccaacgcc gcggaagcac gcagcgcccg caaggaaaac aggttcgacc
     1141 tggttctgct cgacatctgg atgccggaca ccgacggcat ctcgctgctg cgcgaatggt
     1201 ccgaacatgg cgaactggaa acgcccgtgg tcatcatgtc cggtcacggc actgtcgata
     1261 ccgcggtgga agcaacccgc ctcggcgcac tcgacttcgt cgagaagccg ctgtcgatcg
     1321 caaagttgct gcggacggtc gagcgcggcc tggagagtgc gaggaagcag gcaggctccg
     1381 gccgcgggat gctgccgtcg ctcctcgcgc cggtagggcg cagcaagctg atgcaggacc
     1441 tgcgggacaa ggttcagcaa tacgccaatc acgactcgcc ggtgctcctg atcggcgaac
     1501 cgggcaccgg gcgaggcgca ttcgcgcgtt acattcacgc tttgagcccg cgtgcagagg
     1561 agccgctgga agtactcctg gccgcctccc tcaccgaaac gaatacggaa gagcagctgt
     1621 tcggcgtcga aaagaacggt gaggtacgtc cgggtttttt cgaacgcgcc ggaaacggaa
     1681 cgctgctcat cgaggaacta tccgacctca atgaccaggc gcagaagctc ctcttgagcg
     1741 cgctggagca acgctggttc acgcgcgtcg gcggccacga gcaggtcgag ctctgctcac
     1801 gcattctcgc gtcggtgcgc gacgattacg aaagcgcaat cgaacgcggg cagctacggc
     1861 gcgagctggt ttcgatgctg aatgtcctga gcgtgcgggt gccaccgcta cgcgaatatg
     1921 cagaggacgt accggagctg ctgcactact acgtggacaa gctggtcgat tccgaagggc
     1981 tggcattccg ccgcttcagt gtcgcggcgc agaaccggct gcgcaactat ccctggcccg
     2041 acaatgtaag ggagctcaag aacctggtgc gccggctgtt gctgaccgga tcgggcgagg
     2101 agattgcgct cgacgagatc gaacgggaac tcagcgcaag ctcacagagc gacgagccgc
     2161 tggtgaagca ggacctcctc tcgctgccct tgcgcgaggc gcgggaacag ttcgagcgcg
     2221 cttatctgca gcaacaactc gtcctgtgcg acggtaaagt gggcaaactc gcgaagcgcg
     2281 ttggcatgga acggacgcac ctgtacagga agcttcgttc attgggcgtc gatttccggg
     2341 cgatttctac cgcggacgat tgagggcggt ttattccggg acgtgtccat gttcgcggaa
     2401 ggtcgagcga gcctcgggaa ggatgcggcc gttgtccaga aaccggatgc cgggagtgag
     2461 gaagtcctgc cggctcaggt cgcggatcag gtgcaacgca tgacggcttt cgtcgcggtg
     2521 cggcccgccg gggcgtacct gcacggttga atagatctgg ccttctataa accggcccgt
     2581 gccgtcgacg gttacctgca ggattggcgc tacgccgttc aggccgttca cgctgattcc
     2641 gtagtaggtc gcgaagttac cgaggctgta ggcgatgatg cgatcctggt agcgttccat
     2701 ggcacgcacc acgtgcggtc cgtgacccag aacgaggtcg gcgccggcgt ccaccatctt
     2761 cctcgcgaag tacaccacgt cgccgcgcgg ctcgccgtaa tactcctcct cggcgaacgg
     2821 caggcgcgtg acatcgttgc cttcggcacc gccgtggaag gatacgatca cgatgtcgtg
     2881 actcgcgtcg tgcgcgagca caatctctgc agctgcgtcg taatcgtgca gcaaattcga
     2941 cttctgcgtt accgcgaacg ccagcacggc gatcttcaga cccttgacgt cgatgctggc
     3001 gaagtcgccc tcgcgcccgg aatgcgcgat gccggcggca gccagcgtgc gcatggttgc
     3061 agagcggccg ccttcgccga agtcgcggga atggttgttg gcgaggctca tgacgtcgaa
     3121 gccggcgtcg acaaaatgcc ggacgtaacg cgacggcgta cgaaacagga aacatgcaga
     3181 cgggttggtg cagaccttgc ggggagtccc tccgtcgacc agtacgccct ccaggttgcc
     3241 gaaggtgagg tccgctgccg acagcaccgg cgccacgccg gcgaggaagc cggcgccgtc
     3301 atcatccggc aggtgatttt ccgggtagtc ggttccgagc atgatgtcgc cgacggcggc
     3361 gatcgtcagc tcgcgggctg ccgagggtgt cggcggcgcc tgccgggaca cttgctgcgg
     3421 agcgccggtt ttcacgcgtt gttccgctga cggcttcggc ccggcggcgc agccgcccag
     3481 cgtgagcgcg acgatcgcac tgatcgcgag cggccgcctg gctttcgcga acatcagtct
     3541 cccaggcgga ttcgcagcac gtcgatgtcg gcttcccgca accggcttct cacgacgttc
     3601 agttgttcca ggtcctccat cggcccgatt ctcacccggt gataggtctt gtcgtcgatg
     3661 gttacctgct ggatggtcga ttcgatgccc tgcagcgcga gttgcgccct gcggcggtcc
     3721 gcgtccgcgt gctgcgtgaa ggaaccggcc tggaggacgt aaacgccggg ttcgacgacc
     3781 gcctgcaccg ccgtatcggt cttcacgtcc ggctcctgtt ccggaatgat gacttcgaag
     3841 ttcggcagca tgctgtagaa gtcgaaacgg ggttttgccg gctcatcggc agcgccggac
     3901 ggaatttcca ctggcggtgc agcagcctgg tccgtggcct gttccatgct cgcagcttcg
     3961 cgcgcctgcg gctcggccgg aatccgggag ttccggccgt tgatgtaaat ggcgaacgcc
     4021 accgacaggc ccaccagtag cccgaacagc atccatgccc agcccgggta ctcactgctt
     4081 tccgcgcggt gtcttgatct tcgctttgcc attgcttaca tcgattccgg cgcggacaca
     4141 cccagtatgt cgaggccgct tgcgatgacc tgccgggttg cggcgatcaa cgccaggcgc
     4201 gcgttgcgaa ggtcatcgtc gtcgacaata aaggtatgcg cgttgtagta ggtatggaac
     4261 tcgttggcaa gctccctcag gtactgaacc aggtgatgag gcgcgcggtt ggccgctgcg
     4321 gcctcgataa tttcgggata gcggctcacc gagttcatca gtgatttttc gtgcttctct
     4381 tcgagacgcg gaagattcga gagcccgtcc tgctcgtccc acgcgagccc tttctcggcg
     4441 agctggcgga acacgctcgc gacacgggcg tgcgcgtact ggatgtagta aaccgggttg
     4501 tcgttggacc ggctcttcgc gagctcgagg tcgaagtcca ggtgctggtc gtgcgagcgc
     4561 atgacgtaga agaatcgtgc tgcgtccttg ccgacctcgt cccgcaactg gcgaagcgtg
     4621 acgaactcgc ctgaacgggt cgacatcgcg agcttttcgc ccgcgcggta gagcgttacg
     4681 aactgcacca gttccacttc caggctgtcg cctgcgaagc ccattgcttc cagtcccgca
     4741 cgaacgcgcg cgacatagcc gtgatggtcg gcgcccagga tgtccagaag gtggtcgaag
     4801 ccgcgctgcc gcttgtcgta gtgataagcg atgtccgacg cgaagtacgt cgtccggccg
     4861 ttctcccgca cgaccacccg gtccttctcg tccccgaagt cggtcgcacg aaaccaggtc
     4921 gcgccctctt tctcgtaaag catctggcgc tttttcagca cggccagggc ttcctctatg
     4981 cgccggtcgt ccgagaggct tcgctcggaa aaccagcggt cgaactcgac gccgaattcg
     5041 ccgaggtcat ccctgatgtc gtcgaggatg gagtcgagcg cctggcgccg gatggagata
     5101 aacctctccg ctccaaggag ctccgtggcc gccgcaatta ccgcagtgat gtaggcttcc
     5161 ttgtcacctt caggctcatc cgccggcagt cgctgcttca gttccgccgc ctttaccttt
     5221 ggcagtcccg gagcgtcgat agcctcggca atctccttta tatagtcgcc gcggtatccc
     5281 gctctcggga aggcaacaat ctcgccgtcc cgctccagca tccgcagcca cacgctcgcg
     5341 ccgaggatgt ccatctgccg gccctcgtcg ttcacgtagt actcgcgtcc tacggaatag
     5401 cccgctgcct caagcaggtt gccgagcgtc gcgccgtacg ccgcgtgccg gccgtgtccg
     5461 acgtgcagcg gcccggtggg atttgccgat acgaactcca gcaggatgcg tggctcggct
     5521 cgggtagcct gcctcccgta cgccgatcct tcccgcagca ccgccgcaac ttcctgctgc
     5581 agcgccgttt cgctcaggtg gaagttgatg aagcccgggc cggcgatctc gaccttctcc
     5641 acgaggtcgc tggccggcag gagcgcgatg atctgccctg ccagttcgcg aggcttgctt
     5701 ccgaccgctt tcgcaaggcg catcgcgata ttggtggaaa aatccccgtg ccgcgggtct
     5761 cgcgtccgtt cgacggtcgt gcgcgtcgac gttacctcgg gggaatcagc cagctccgga
     5821 aggccagcaa tggccctgtc gaccagttct gcaatactgc ttttcaacga cgaatctctt
     5881 tgatcaagtg cggatgatgt gggcggccgg caattctagc agcacttaga acaactcgac
     5941 cgggtcgaca tcgatggacc agcgaacctt gcgggcccat ggcccggctt cgagttgcgg
     6001 gcgcaccgcc tgcaacaggc ggtgcagggg ctggcggtgg cggctctgga agagaagctg
     6061 cgcccggtag cgtccggcgc ggcgttccat gggcgcactg acgggtccga ggatccggac
     6121 accttcgacc ttggccgctt ccgccgtgcg acgggacctt gcgaggaagt cgtgggtgtc
     6181 ctgccggcgg gtggcggcgg ctcgcaggag cgccagtcgg gaaaatggcg gccacgctgc
     6241 gttctcccgc tcggccagtg cgctcgcggc gacgcgttcg tagcctccct ggaacagttc
     6301 atgccagaac gggtgggagg cgaaggaggt ctggatcatg acctctccct gccgttgctc
     6361 gcgcccggcg cgcccggcga cctgcatcag gccctgcgcc aggcgctcgg ttccgcggaa
     6421 atcggtgctg aagaggccct gatcggcgtc aatcaccaca acaagcgtca ggttgggaaa
     6481 atgatggccc ttcgagagta tctgcgtacc gatgaggatt tccgtctcgc cggtagtagc
     6541 catctcgagc gccttgtcca gggctccact tcgacgcgtg ctgtcgctgt caatccggct
     6601 gacgagcgca tcgggaaagt ggagttgcag cgcgtcctcc agccgctccg tgccatgccc
     6661 cagcggcttc accgtgccgc cgcattccct gcaggcaggg tccagtgcgc gttgtgctcc
     6721 gcagtggtgg catatgagcc tgagacttcg ggcgtgaacg gtcatgcggg catcgcagcg
     6781 gctgcattct gcaatcgcgc cgcaatgctc gcagatcagg gtcggcgcga agccgcgccg
     6841 gttcaggaat accagcacct ggccgccttc ggccgagtgc cggcgcatcg cctcgattac
     6901 cgggcggctc agcccgttga gtggctggtg ccggttcagg tcgacgagcc gcagcagggg
     6961 cgggacggcg ttgcctgcgc gcaccggcag caccaggcgt tcatagctgc cctcggcgca
     7021 cctctgcagg gtttccagcg agggggtggc cgagccgagt atgaccggga tgtccagttc
     7081 cttcgccctc gccacggcaa gatcccgggc cgagtagcgc agcccttcct gttgcttgaa
     7141 ggaactgtcg tgctcctcgt cgacgacgat caggccggct tcgttcaacg ggacgaatat
     7201 cgcggagcgg gtgccgacga ccagccgggc gctaccgtcc ctcgcggcgc gccaggcatc
     7261 gaggcgctgc gtatcactga gcgcggagtg caggatgaca ggcagcacgc cggagcgctt
     7321 ctttagcctc gcgacgaact gcggagtcag tccgatctcc ggcaccagca cgagcgcctg
     7381 ttttccgcgt gcaagcgttt cttcgatgag acgcaggtac acctcggttt tgccgcttcc
     7441 ggtgacgccg tcgagcaggc ttacgccgaa cctggtccgc cccgagatcg cggcgaacgc
     7501 tgccgcctgg tcctggttca gcggcgggcc tccttccgca tctgctgaat cggcgcttgc
     7561 agtcgcctcg ccccgggatg cggtcacttc tacccagccc ttctccctga agctcttcac
     7621 aaggcgcttc cagcctggcg cgagctgccc gagggattcg aagttcaggc atcccgcctg
     7681 ttgcagcagc gacaggagtt ccgcctgctt cggcgctttc ttcgcgaccg cggtggcgtc
     7741 cgcctgcctc ccggcatccg tcagcgacag gtgccggatc gtcggatgca gcggcttgcc
     7801 ctggcgcagc agtgcgggaa gcgccgcggc gacgacttct ccgacgggat ggtggtaata
     7861 ctcgctggta aagcgaatca gccacaggtc ggaggggcgg aggagcggct cttcgtcgat
     7921 gatctcgtgc gcgcgcctta gctttgttct tgcgatttca ctgtggcctg cgacctcgag
     7981 cagcatcccg gtctgcgtgc tgcggccgaa cggcacgcgc agccgactgc cgggctggag
     8041 actatcgtta ccggccttcg gcggcaggta atcgaacaac cgtgacaacg gcgcgttgat
     8101 cgcgactcgg aagactggtt cgcggctcat ccatcatccg aaaacgttac agtgatttca
     8161 cggcctggat cagctccgac gccattgcct ggccgtcgcc gtagagcatg cgcgtctggt
     8221 cgaggaagaa gagcccgttc tcgatcccgg aaaatccggc gccctggccg cgcttgatga
     8281 cgatgacatt cctggccttg tcggcattca ggatcggcat gccatagatc gggcttgcag
     8341 ggtccgtccg ggcaaccggg ttgacgacgt cgttcgcgcc gatgactagg gccacgtcgg
     8401 ccgtggcgaa ctccgcgttg atctcgtttt cgtcatagat cacgtcgtag ggcacgccgg
     8461 cctcggcaag caacacgttc atgtggcccg gcatgcgacc cgccacggga tggatagcga
     8521 atttcacctc gacgccgcgt tcgatcagga tctgtgcaag ttcccagacc ttgtgctgcg
     8581 cctgcgcgac ggccatgccg taaccgggaa cgatcaggac cttgttcgcg aacgccatca
     8641 tcacgcccgc gtcgcttgcc tcaatcggct tcatgctgcc gtgcacttcc gaggcaaccg
     8701 ccgccgtatc gccaaaaccg gagaaaagca cgttcccgag ggaacggttc atcgccttcg
     8761 ccatcaactg tgtcagcagc gtgccggcag caccgacgac cgtgccggcg atgatcatgg
     8821 ccgcgttctg cagcacgaag ccttcgaagg cgacggcgag gcctgtgagc gcgttgtaca
     8881 gcgaaatcac gaccggcatg tcggcaccgc cgatcggcag cgtcatcgta acgccgagaa
     8941 cgagcgccaa caggaagaaa atcgcaatga gagccggccc gccgtgctgc gtgacgatca
     9001 cggttgccag cacgagcaac acggcgagca gcaccaggtt gaaaacctgc tgcccgccaa
     9061 aacgcacggt ggatttcatc aggccctgca gcttcgcgaa cgcgatgagg ctgccgctga
     9121 aggagaccga gccgatgaga ccgccgagga cggcaaggac gccgaaggtg agtccgtgat
     9181 cctcgccgcc gaacagttcg actgcggcga tagcggccgc cgcgccgccg cccatgccgt
     9241 tgtagagcgc gatcatctgc ggcatgtcgg tcatggccac ccgcttgccg ctgacccacg
     9301 caagggtgcc gcccaacgct atggccacta tcatgaggac atagttgtcc atccccggcc
     9361 agaggaatgt caccagcgtc gccacgagca ttcctgcgcc tgcccagacg atgccgccgc
     9421 gcgccgtgac cggcgaactc atccttttca gtccatatat aaagagtatg gctgcgacga
     9481 agtaactggc ctcgatcagc cagcgcagcg aagaagcgtt atccatcagc gcttctcctt
     9541 gctgctcttg aacatttcga gcatgcgttc ggtgacgacg tagccgccaa cggcattgcc
     9601 agcagcgagg aacacgccga tgaagccgat cgtcatttcg aggcccgtct ccgcacggcc
     9661 gagcgcaacc atggcgccga ccagcacgat gccgtgaacg aagttcgagc ccgacatgag
     9721 cggcgtgtgg aggatgaccg gcacccggcc gatgacctcg tgcccgacga acgccgccag
     9781 catgaaaatg taaagcgcga tgaagccgtc aatcatcgat ctgtatcctg gtcatctgtt
     9841 tgctggtgtc ctgtaacagg cactattggt tctttgcttc aagtgactgg cgggtgcctt
     9901 cgtgccttat ctcgccatcc cgcgtcagca ccgtcctcgc gaacacctcg tcttcccaat
     9961 cgatttgcaa ttcgccttcg ctgatcgccg gactcaggaa attgaacagg tttctcgcgt
    10021 acatctcgct ggcatgcctg ccgagcttcg ccggcaggtt gaccgggccc acgattttca
    10081 ccttgttctc gaggaccgtt tcgccggcac gcgtgagctc acagttgccg ccggtttccg
    10141 ccgccaggtc gacgattacg gctccgggca tcatcgtgcg caccgctgcc gccgaaatga
    10201 tcctgggcgc cggcctgccg ggaatcgccg cggtgctgat tacgacgtcc gatactgcga
    10261 tctccttctc gagcgctgcc tgctgcttct gcttttcttc ttcggtcagc tcgcgggcat
    10321 agccgcccgc cccttccgcg ctgacgccgg tttccacgaa cttcgcaccc agcgactgca
    10381 cctgttcttt cgtggcgccg cgaacgtcgt aacccttgac gaccgcgccg agccgctttg
    10441 ctgtggcgat tgcctgcagg cccgccacgc cggcgccgat taccagcacc tgtgcagggc
    10501 ggatggtgcc ggctgcggtg gtgagcatcg ggaagaactt gtcgagcgta ctggctgcga
    10561 tcagcgccgc ctggtaacca gcggcggcgg cctgggaaga cagcacgtcc atggtttgcg
    10621 cgcgggatat ccgcggcacg agttccatgg cgaaggcggt gtggcgcttc tccttgagta
    10681 catccacgag gtctgcgtgc agatggccgt aaacgaaacc tgccgtgacc gaaccctcgc
    10741 cgagctcccg gaccgtctcc gccgacggcg gctgcaccgt aaggagcagt ccgcacttgc
    10801 tcgttacccc gacggcatcg gtgaactcga cgccctcgta ggctgagtcg agaagctgtg
    10861 accccgctcc tgcgtcccgc tccatcacta cggagacacc cagctttttc aacttggtgc
    10921 tgatctcggg ggtcagggca acgcgggttt cgccctcgag tgtttccttg agcacgccaa
    10981 cggtcagcgg cattaatcgt ctccggctgg tccttaacta cagtaaagaa tgcttattgt
    11041 tgggacaggt ggccctgttg acgcgatgtg atacgtgtgg gaatcgcgcg aagcgcgaat
    11101 gatgacatag gagtctcgag tgtagaaagt tttcgtgccc gggatatggc tcgtcgttct
    11161 gttgttctta tgcctcactg ttgctttttg ccggcttcct tctgcaaact agacagtctc
    11221 agcacaagcc tgagatattg atttatgcgt tttggtgcag cttcagacat ctcgcaacag
    11281 gtcttgcgaa ccgacgtggc gggaatgcca ctcgaatgga tcgattaccg tgaagccgta
    11341 aggctctatc acacggagca ggttgcgtac gcctgcggca cgcggcttta cacgatcttc
    11401 ggcggttaca gtgcgctcag cggacggcgc agcgccatcg atgtgagttc catcgtcgcg
    11461 acgcgcggca ccagccaggg attggcctac gtccgtgacc agtacgtacc acctctcaac
    11521 aatcgcacgc ttttcaaacg ggacgccaat ctctgccttt attgcgcgac ccgatttccg
    11581 acccgcgacc tcacgcgcga tcacatcacg ccgctgtcgc agggcggacg agatgcgtgg
    11641 aacaacgtcg cgacggcttg caagcgatgc aacaaccaca agggcgggcg aacgccggaa
    11701 cagtctgcga tgcaacttgt agcagtgccg ttcacgccga cctatgcgga atacatttac
    11761 ctgaaaggcc gcaaggtcct ggccgaccag atgaaatacc tgctggcgca catcccgcgc
    11821 tcgagcccgc tgcacgcgcg gctcagcgac catctttccg acggctcgct gttcctcgat
    11881 tcctgattcg tactggtcaa ttccatgccc gtcccggcat ccgggaactg ctatcatcac
    11941 ggcacatccg ccgttcccgg aaagccatcc ataaatgcag tacctgatcg acgccagtgt
    12001 ctatgtcttt cgcgcgcacc actcgctgcc ggacgacatg gtcgacggcg acggcaaccc
    12061 ggtcaacgcg ctatacggtt tctgcaggtt catcggtgat ttcatcgaac aggtgcgccc
    12121 cgaatacctc gcggtgctgt tcgatgagtc cctcgaggcc tcgtttcgca gtgagatata
    12181 cccggcgtac aaggcgaacc gcgatcccgc accggtagag ctgaaacgcc agttcataca
    12241 atgccgtgaa ttcacgcggg cgctcggcat catggagtgc gctcacccgg cgttcgaggc
    12301 cgacgacctg atcggcactc tggtcgaagc gggacggcgc gcggggcgag catccacgat
    12361 cgtaagccgc gacaaggacc tggcgcagct cgtctcggaa caggacgtgt tctgggattt
    12421 cgccggcaag ggcaagctca cctacgacca gattcccggc gtgttcggcg tgtggccgga
    12481 acagatcgcc gatttcctcg ccctggcggg tgacagcgtc gacaacatcc ccggcgtgcc
    12541 aggtgtgggt aagaaaacgg ctgcggcact gctcaagcac ttcggcagcc tcgacggcat
    12601 atacgcaaat ctcgatcggg tgcacgaagt ctgctgtcgc ggcgcgagaa cgctcgcact
    12661 gaaactcgca acgcaccggg aggcggcaat gcttgcccgg cggctgacag gcatcgcatg
    12721 cgaagtgccg ctcgagcggg cactgtcctg tctcaggccc gtggcccctg accttggcgc
    12781 gatcaacgcg ctgttcgatc gggcatcgat cggcatggcg ttgcgccgcc aggcggaacg
    12841 gctgtcggac ctcgttgccg aaccaaacct gccacggctg aatttgcagg cggcgtattg
    12901 agagcctgcg aagccaccat tagcgattga cgtcgtgacg acagtcgatg tccgactggc
    12961 atgacctgaa gcaagccctg cggaaacaag gcatcgcaat tgccgatgcg aagccgcgtg
    13021 ctgtcggcgg cggcgatatc ggttccgcct ggaaagtgcc taccgatcgc gagccggtgt
    13081 tcctgaagac ggggccgcgt gagtttctcg aaattttttc ggcggaggca gaaggcctcg
    13141 aagaactggg atcggccaac gccgtgcggg tgccggcggt gcttgcgttc gggctggccg
    13201 ggagcgatgc ctacatcgcg ctcgaatggc tggatctcgg cggccgtgat accgcggctg
    13261 cgatcctgct cggcaggcag ctggcgttgc agcatcgcaa tctcgacgaa cgctttggct
    13321 ggcatcgcga caacatgatt ggcaccacgc cgcaactgaa cgatcgggac gacgactgga
    13381 tccgtttctt ccgcgagcgc cgcctgctgg tgcaactgca gctcgcggaa agtaacggct
    13441 tcggcgggga gttgctgcag gagggggcgc gcctcgcacg taacctggat cttctcttcg
    13501 acggttatgc gccggttccg tcacttcttc atggcgacct gtggggcggg aactggtcgt
    13561 cggtcgcggg cgagccggtg atattcgacc ccgccgtgta ctacggcgat cgggaatccg
    13621 acctggcgat gacgcgcctt ttcggtggct ttggcagcgg cttttatcgc gcttacgagg
    13681 atgagtggcc gctggccagg ggacacgaga agcgagtggc gctttaccag ctctaccatg
    13741 tcctcaatca cctgaacatg ttcggcgttg cgtacctgtc ccgggcgatg agcctgatca
    13801 gggagttaca gcgctgaagt caggttcagg accggcggcg cactcgtggc gcagcaggtc
    13861 gcggtagagg ccggagagtt tcgtggtcac tgacttagaa gcgccggcgg cgaattcgcg
    13921 cccgtcgagt gtagccaccg gcgtgacacc accgaacgtg ccggttacga aagcctcgtc
    13981 cgccgagtaa gcgtctgcca gtgtgaaatc gcgttcctcg actggaatac cgtgttggcg
    14041 gcagagctcc accacgtggc cccgggtgat tcccttgaag caaaagcgtc ccgtggatgt
    14101 ccagacggcg cctccgcgca cgatgaaaaa gttggttgcg ttgcaacttg cgacgtgacc
    14161 gtaagggtcg agcatcagag cttcgtccgc gccaagagcg gcggcctgga tcagtgactg
    14221 gatcaggttc agccggctgt gagtgttcag gtgcatatcg aagacgtcgg gcgtgctcgt
    14281 ccgatacgga gaggtgacca gcaccagtcc tcgttcccgg ctttcgggat gcggctcctt
    14341 gtgctccgcg atgatgacga tcgtcggacc cgaaatcgcg gcccggggat cctggttggg
    14401 agtgatcttg atgccgcgcg tgaccatcag ccggacgtgc acgccgtcgc gcatttcatt
    14461 cgactcgacc gtccggtaca acgctcgtgc catctccttg cgatccatgc cgatatcgat
    14521 cgccagcact ttcgctgcgg aataaaggcg atcgaggtgt tcatccagga acgcgagccg
    14581 accgttgacg agccgtagtc cctcccacac tccgtcaccc agcacgaaac ccgcgtcgaa
    14641 caccgagacc ttggcttcac tgcgcagcac cagctcgccg ttcagccaga ttttcacctg
    14701 ctcgttgcga ggatcgggaa cgaaaccctg ggaaccgacc attactgtcc ttccaccggg
    14761 atgcggtgct gcgccagtcg ctcgtaaaag ggaagtgctg cgtcgatcac cggcaggagt
    14821 tccggcgcgg gctgcgcctg ccgacgcgcc gggccgaagc ccgaggaacg ccagaccgac
    14881 tggtaccaat gtggcgccca caccccatcc gtctcgcgcg gcccctccgg ccaggaaagc
    14941 atgcgctcgt cgaaggggac tcgcagcgcg ttgcatagcg cgcgaagcgc aggctccggc
    15001 ctctcgagca cgtccctcga atcgaagacc ggcggcgctt ccccgcgcgt gtccgctatc
    15061 ctgtcgaaca actccacctg ccgcacatag cccagttcgt cgagcgaagg cacgctcttt
    15121 aaagcgtgtg cccatgaggc gctgacttcc tgcgggtgac ggatcagaaa tgcatgcgac
    15181 actcgttcga gccattcgag tggtgcctcg ggcagcatgt ggtgcgtcat gtgcttctgg
    15241 tagaagatac gccgcccccc gggggtttcg gccgtgagct ttcgggctac agcatgccag
    15301 tcggaagact ggccggcgag cacctcgtcg cggaacgggt gcttcgcgcc gctgaacttc
    15361 aggtaacagg cgtaaaacgg ctcgtccacg actgccgtgt ccggccggtt ctcccacgcg
    15421 cgcatcatcg cggtcgacag gttgcgcggc ccggaccaca tggcgatgcg gacgctatcg
    15481 tccccggtga ttggctgact catcgatctg atcgtaatcg cagtacgtgg tcggtacgac
    15541 cgtgttttgc ctcaactccg tttcgcgaag gtgcacgctt gatagccgcg tccgccctgc
    15601 gaagatttct ctaccgatcc acgtggtcga tgagaccttc cagtatcacg gcgatgcgct
    15661 cgtgccgctt cccgacggac cagcgcaggc agggtatcgc aacagcggcg ctcgccagcc
    15721 ctacgacgac agggaggaat acgccatacc cggcgtccat cgccgtgaaa gcggcagcga
    15781 gaatcgcggc gaccccgacg cagctgccgg cggtgtagag cgcctcttcg cgcttgttcg
    15841 caagatcggc cgcaagcgtc accagcgacc agcccggttc cgtttcagcg atcaccagtt
    15901 caacctggcg ggtgtcggcg agcgcgtact ccttgccgga gaaattcatg aagcggtcaa
    15961 gccggtccat gatgcctgcc gaagcttccc agaccgaacg ggcaggtcgg cgtcgcagcg
    16021 cgctcagctt ctcgcgttcg cgcaacaaca tctcgacctg ttgctggatc agcgtcggct
    16081 cacccgccac cacccggcgc acttcaacac gcgcatcccc ggcaaccagg tccaggatcc
    16141 ggttcccctg cggtcgttcc ggcgccagcg attccgcgtg cacctcggcc atcgctcgac
    16201 gcacgtgcgc gggctccagc cccacctgcc tgccgatctc gaccacttcc tcctccgtga
    16261 gcagctgtga ggccgtgttg ccgcgcttgt tctggagctc ggcggcacgc cgcacgatca
    16321 ggtcgaggtc ctctgacgca acctggcgtc gcgacggcag cgcgcgatgg tcctgtggct
    16381 ggcgcctggc attgaggctt tccacctgtg cggcaggctt ttgcgggtta agtgtttcgg
    16441 cggggctctt ttcctgtgcc attgttttct ccgatggtcg ggccgtcagc actggttcga
    16501 aacagcaagt cgcgtgccag atgccgcaga aggcatgaac gcttgcatct cgccaaggcc
    16561 ggcctccttg ccatggcgaa aggcggcaac tcccggggga aatcgccaca tgttagccga
    16621 ttctgctcgc ttctcctcag gaagtccgcc gtgcgaagat ctcgcgcact gtctcgatcg
    16681 tgtcggcatc ttccggccgc ttgtcctcgc ggtagcgttt cacccttgcg aatctcaagg
    16741 ccatgccacc ggggtactgc gagctcgact gaagctcgtt gaacgcgatc tcgaccacca
    16801 gctcgggacg gacgtgcacc acgtgacctt cgcggccggc ttcgagttcg agcagccgct
    16861 tggtctgcca ttcgagcatc ttgtcggtca ttcccttgaa ggtcttgccg agcatgacga
    16921 acccgttttc ctcatccgcg ccgggatcgc gggcggcgag atggaggttg ctgagccagc
    16981 cgcggcgccg gccgctgccc cactcggcgg caatgacaac gaggtccagg gtatgcgccg
    17041 gcttgatctt gagccagccc gctccgcggt tgccggcttc ataaaggctg tcgagcgact
    17101 ttgccatcag tccctcgtgt cccgcagcca gtgcctcttc gaggaaaccg gcggcggcat
    17161 cggggtccgc cgtcacgagc ctcggaatga ggaatgcagc cggcacaact ccggacatgc
    17221 gctcgaagcg ttcaaacgcg gggagatcga tcaggtcgtc gccatccacg tgcaggcaat
    17281 cgaagtagaa cgcgtccaac ggaagcgatt cccgcaggcg ctcgacatcg agggtacggc
    17341 cgaagcgccg catcgttacc tggaacggat gtggcgtgcg gtcgttgcgc aacgcaatga
    17401 cttcaccatc gaggatcagc ttacgcgcgg g
//
LOCUS       DASUOM010000634        12823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_455176,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000634 DASUOM010000000
VERSION     DASUOM010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455176"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFY27_13305"
     CDS             <1..303
                     /locus_tag="VFY27_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5766537.1"
                     /translation="AVKAIAVGLLLMAAIFQVADGIQIGAAGALRGYKDTRIPMVINT
                     FAYWGLAFPLAYMAAITFRAPPSYIWGGFVLGLSVAALLLTIRFGRVSRKESLLSA"
     gene            complement(318..986)
                     /locus_tag="VFY27_13310"
     CDS             complement(318..986)
                     /locus_tag="VFY27_13310"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5766538.1"
                     /translation="MMPVKLLSESMDATITDDDHARIERILSFWFKEHELSAPQIDGR
                     MDTWFGEDPVFDHEIEKEFARDVEDASTGAIDYWAHQPRGRLALIILLDQFRRNIYRG
                     TAEAFAKDKVALKLCVEGAMKKKDKGLTPIQRVFFYMPLQHAESRKVQAKSVEIFQRL
                     AKAVSPTYRETFETVAHFAELHHDIIQQFGRFPHRNKVLGRKNTPEEDEYLSGDSPNF
                     GQGG"
     gene            1133..1618
                     /locus_tag="VFY27_13315"
     CDS             1133..1618
                     /locus_tag="VFY27_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX5766539.1"
                     /translation="MNMKLPGSMSRYIILCAAALLASCAAPKLTVQPPAVTLTGIEVR
                     NIGISGQTFLLGFAVSNPNPFPLPVKNIRYTVMLDGQRFAGGETESDFVVSARGDGEF
                     RIGVEMDLLQSVSQLSTLFRGGLRDTLEYDMDGSLAVDIPFSRPIPFSSKGVIKVRPY
                     N"
     gene            2076..6539
                     /gene="gltB"
                     /locus_tag="VFY27_13320"
     CDS             2076..6539
                     /gene="gltB"
                     /locus_tag="VFY27_13320"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX5766540.1"
                     /translation="MSGKTTTGTGLYDPAFERDSCGFGLIANLDDKPSHWLVDTAVTA
                     LARLTHRGAVASDGKTGDGCGLLLKFPGQFFRSIAAGCGITVAERFAAGTVFLSQDEA
                     VAREVKRTIDQALEDVGLAVAGWREVPVDPSVCGVQALQTLPRIEQVFVNASAGMQHT
                     YFNRRLFLARRRAESRFSKIDSSAYISSLSSATISYKGMVMPDVLPEFYPDLKDPRLE
                     ASVCVFHQRFSTNTLPEWRLAQPFRFLAHNGEINTIQGNRNWAVARAANFRSEKLHDL
                     SDLDPIVALNGSDSSTLDNMLEVLRAGGMDVLQAMRILIPPAWQTVDSIDPDVRAFYE
                     FYDCHMEPWDGPAGIVLTDGHYAACCLDRNGLRPARYVVTKDRHVTIASETGVYDYEA
                     SDVTAKGRLGPGEMLAVDLRNGRLLTTQDIHQDLKARAPYKKWLKQGVKYLDSELVDR
                     HIAAEPMDEATLATYQKMFNLTREELNEIVSVLAKAEQEAVGSMGDDTPMPVLSKQIR
                     SLFDYLRQQFAQVTNPPIDSLRERTVMSLQTNIGREMNLFDIGPEHAEQVVMNSPVLS
                     QRKLRQLLGPDMFGGSHAFVDLNAPEEQDLAGALDRICAESMQAARDGKLVIMLSDRY
                     LKKGMLPVHALLATGAVHHSLVASGFRCAVNILVETGVARDAHHFACLIGYGATAVYP
                     YLAYQSLHDLGQRGNVDRHRQLGRSYRRGIRKGLFKIMSKMGISSISSYRGAQLFEIV
                     GLHEEVVSKCFKGTVSRIQGAKFADLLEDQQQLGATAWEPRLPLKQGGLLKYVHDGEY
                     HCFNPDVVATLQAAVRSGDFGRYLDYSKLVNERPVSTLRDLFALRPAGPAIPLSEVEP
                     VSSILPRFDSAGMSLGALSPEAHEALAIAMNRIGARSNSGEGGEDPARHGTERMSKIK
                     QVASGRFGVTAEYLVHAEVIQIKIAQGAKPGEGGQLPGHKVNEMIARLRYAKPGVGLI
                     SPPPHHDIYSIEDLAQLIFDLKQVNPQALVSVKLVAEPGVGTIAAGVVKAYADLITIS
                     GYDGGTGASPLSSVKYAGSPWELGLSEAHQVLCANDLRQKVRLQTDGGLKTGMDVIKA
                     ALLGAESFGFGTAPMVALGCKYLRVCHLNNCATGIATQNNVLRSEFFVGLPDMVVNFF
                     RFVAEEVRMRLADLGLRRLEDIIGRVDLIELKPGETRRQKQLDLRPIVSSRGLVSDSP
                     QFCTDARNVPADKGELAERMVKEILPAIEAKSGGSFSYGVRNYNRSIGARLSGEIARR
                     YGNHGMQDAPVDIRLKGTAGQSFGAWNVAGLNLELIGDANDYVGKGMAGGRIIVKPPS
                     DAGYAAKDTVIVGNTCLYGATGGQLFAAGLAGERFAVRNSGAVAVVEGAGDHCCEYMT
                     DGVVVVLGRTGINFGAGLTGGFAYVLDLDRDFVDRYNHELIDIHRVTSESMEAQLQHL
                     RATITEHVAMTGSEWGIAILDDFRTYLPRFWLVKPKAAELGSLIRSLRQAA"
     gene            6552..7973
                     /locus_tag="VFY27_13325"
     CDS             6552..7973
                     /locus_tag="VFY27_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019571641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5766541.1"
                     /translation="MSKHPLQFLEVPRRDPEKQLAEARIRHYDEIYGSYDGADAASQA
                     GRCIGCGNPYCEWKCPVHNYIPDWLRLIEEGKLFQAAELSHQTNSLPEICGRICPQDR
                     LCEGACTLNDGIGAVNIGNIEKYITDEALKLGWRPDMSHVPDTGKRVAIVGAGPAGLA
                     AADVLARLGIRSVVYDAYPEIGGLLTFGIPPFKLEKQVVQRRRSILEGMGIEFELGTR
                     IGDDRPFGELLDNFDAVFLALGAYTSVSGDLPGEHLPGVHDALPYLVSNVCREMGIGS
                     GVAEAAQPWVDLAGKHVVVLGGGDTGMDCVRTAIRQGAASVTCAYRRDEANMPGSRRE
                     VANSKEEGVRFLFNRQPVEIIGDGRASGVKLVETRLGAPDASGRRRPGTVPGTGEVLP
                     ADAVIIAFGFRPSPPAWFDEFGIELLPSGRVRVATIGSTPFQTTNPKVFAGGDMVRGS
                     DLVVTAVYEGREAAKGIASYLHV"
     gene            complement(7990..10035)
                     /locus_tag="VFY27_13330"
     CDS             complement(7990..10035)
                     /locus_tag="VFY27_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766542.1"
                     /translation="MNLRRQLLLVSLLTLVLPWAGCQFVRETESALREGQQQMLAGTA
                     QAIADSLAQFPAEFAPREGNAGRRGDLMDKTGDELYGHSLATAPLVDGYLDDWGLAPQ
                     SLVDLPEASASLAVGVYRQSVFLYMDVRDGNVVYSTPGSDRGRSADSIELVTVAEDGI
                     SPLTYIFTPEAPGNLVATLVADGNPDETRISAYWLDTVAGYRLEARVPRQLLRGRLGV
                     VVNNTDNAGARAVRTRSFHGDLPGRLVTVSPLLQSVAAGYVQPDLRLTVLDVVGWRLA
                     QVGEFASRDDAAPGIAAGWMRIAYDALLESGEETELADPDPSGREQQEYVQYALRGEM
                     ASSWFRNADGSRAVVAVAQPVWSGNVQTGAVILQQGTDAILSLRNRALTRLMNFTLMA
                     TLLVAAGLLGYASWLSLRIRRLSSAAERALDDKRVQPELPSAGASDEIGDLSRSFSSV
                     LRRLGEYNEYLRTLASKLSHEMRTPLTIVTSSLENLEHEPLSAESAMYTERAREGTAR
                     LHRILNAMSEASRVEELIEQAETEVFDLNAVLSSTVTAYADAWPLRRFTYLSNASSAP
                     MEGSPELIIQMLDKLVDNAVGFSADGDEIVIRLDREHSGFTLSVSNPGPPLPGRMRSQ
                     LFDSLVSVRSDDTGKHLGLGLFIARLVATGHGGSISARDVDGGAIFEVRLPAARDLK"
     gene            complement(10152..10847)
                     /gene="pdsR"
                     /locus_tag="VFY27_13335"
     CDS             complement(10152..10847)
                     /gene="pdsR"
                     /locus_tag="VFY27_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEX5766543.1"
                     /translation="MSKRIAIVEDEAAIRENYAAAFRREGYSVDTYEARRPAMDAFAA
                     RLPDLVLIDVNLKDEVEGGFELCRDLRSSAPELPIIFLTARDSEFDAVSGLRLGADDY
                     LTKDISIPHLMARVAALFRRLEAMQQPQEKSSRMTRGDLELDVDRMTVRWKDQPVGLT
                     LTEFWIVHSLARYPGHVRNRQQLMDAARAVLDDNTITSHIKRIRRKFLAIDSAFDAVE
                     TVYGMGYRWLGER"
     gene            10984..>12823
                     /locus_tag="VFY27_13340"
     CDS             10984..>12823
                     /locus_tag="VFY27_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HEX5766544.1"
                     /translation="MCAKITTWLVEAAFLAAMLLTQATARAEPAGDAFASPHEMQSGS
                     LLLRMEAGYTIATRINTDIEIQVSGPVARTIVSQEFRNDGAKWVEGVYVFPLPDSAAV
                     DRLRLRIGERFIEGEVREKEEARVEYEKARQDGKRAGLVEQQRSNLFTTSVANLGPGE
                     TVNVEIEYLETVKYDNGQFSLRFPLTLTPRYIPGVGQGRQGSGWSADTRQVPDASMIT
                     PPVVTASRDHAVTMRAEIDAGIPLQVIASRYHPVRVEAVDEAEGRYTVALTGTKVSMD
                     HDLELAWRPVEDAVARATLFREVKDGRPHYLLMVLPPDDMELARADAANPVARELVFV
                     IDTSGSMHGASIDQAKQALLLALDGLAPGDSFNVIQFNSIVDVLYPQSVSATPGNIRL
                     AREYIRKLAANGGTEMYPALERALVPAGDEARLRQIVFITDGSVGNEQELFRLIEAKL
                     RSSRLFTVGIGSAPNSLFMRKAAESGRGAYTFISALHEVKEKMSALVRKIEQPRVTGI
                     QVAWPGEALAYPVVVPDLYAGEPVVQKVRLTGESRGGDVVRISGTSSMGDWGVDLPIA
                     AEQENEGVAAIWARSRIEHLLDRERGGEDAEAARAEVIETALDHQLV"
BASE COUNT         2394 a         3965 c         4108 g         2356 t
ORIGIN      
        1 gccgtgaaag ccatcgcggt gggcctgctc ctgatggcag cgatcttcca ggtcgctgac
       61 gggatacaga tcggagctgc aggtgcactg cgcggctaca aggacaccag gatcccgatg
      121 gtcatcaata cctttgccta ctgggggctc gcctttcctc tcgcgtacat ggccgcgatc
      181 accttccgcg cgccgccttc atacatctgg ggaggattcg tgctcggttt gtcggtcgcc
      241 gctttgctgc tgacgattcg cttcggccgc gtctcgcgca aggaatcgct gctatcagcc
      301 tgagcgctac aacgcgtcta cccaccctgg ccgaagttcg ggctgtcgcc cgacagatat
      361 tcgtcttcct caggggtgtt cttccggcca aggactttgt tcctgtgagg aaaccggccg
      421 aattgctgga tgatgtcgtg atgcagctcg gcgaagtgcg cgacggtttc gaatgtttca
      481 cgataggtcg gtgaaaccgc cttcgccagg cgctggaaaa tctccaccga cttcgcctgc
      541 accttgcggg actcggcgtg ctgcaagggc atgtagaaga acaccctctg gatgggcgtc
      601 agtcccttgt cttttttctt cattgcgcct tcgacgcaga gcttgagggc aaccttgtct
      661 ttcgcgaatg cctcggccgt gccgcggtag atgttgcggc gaaactggtc cagcaggatg
      721 atcagggcaa ggcggcccct tggctggtgt gcccagtagt cgatggcgcc ggtcgatgca
      781 tcctcgacgt cgcgcgcaaa ctctttctct atttcgtgat cgaaaacagg atcttcacca
      841 aaccaggtgt ccatccgccc gtcgatctgc ggcgcggaga gctcgtgttc cttgaaccag
      901 aacgacagga tgcgctcgat ccgcgcatgg tcatcgtccg taatcgtcgc atccatgctc
      961 tcgcttaaga gtttgactgg catcattgcg gccttgtgca tttggaaggc attgatctca
     1021 ttatagtaaa gggaccagcc gggcgccttc cggtattacg tgtgctttag cggggtggat
     1081 atgcgacccc cgtcacggaa tgagaccatg gcgcgcgccc atagtgcctc cgatgaacat
     1141 gaagctgccg gggtccatgt ctcgttacat catcctgtgt gctgcggcac tgctggcgtc
     1201 ctgcgccgcg ccgaaactga ccgtgcagcc acccgcggtc accttaaccg gaattgaggt
     1261 caggaatatc ggcatcagcg gtcagacgtt cctgctcggc tttgcagtca gcaatcccaa
     1321 cccgtttccc ttgccggtga agaacattcg gtacaccgtc atgctcgatg gccaaagatt
     1381 tgccggtggt gaaacggaga gcgatttcgt cgtttccgct cgcggtgacg gcgaattccg
     1441 tatcggcgta gagatggacc tgctgcagtc ggtctcgcaa ctctccacct tgttccgggg
     1501 tggcctgcgg gacacgcttg aatacgatat ggacggcagt ctcgccgtcg acatcccgtt
     1561 ctcacgcccg ataccttttt cgagcaaggg cgtgatcaag gttcgtccct ataactgacc
     1621 ggccgctgca cgcgctgacg ttttccgttt cgcgggtaac aaatacagtc gtcgcgaacg
     1681 ccgccgtaag tgcagaggaa atgccgattg aatcggcaca actgcggggc ctttccaccc
     1741 cgtcttttaa ggcgggtgcg ccaactggcg aaacggagat gcgctccctt atactgagcc
     1801 gctccggttg cttcagctat ccgcgcagcc cgcccggcca tcgaacctgg accagggtcc
     1861 tcgcagactc gtgccagcct tcggcaaggg ctcggccgaa tgactgcgtc cggtaccagc
     1921 agggggagca cggggcaagc aagagcagcc gcgcgtcatc gcactggcga cgatgcaaat
     1981 cgaagcaaac ttgagtgacg attctgtttc gcggcacgca tctcgaagtg cgtgcgcaac
     2041 tgaggcgtgc atagcgtgcg cgcctcggag caaccatgag cgggaaaact acaaccggca
     2101 caggactgta cgacccggcg ttcgagcgcg acagctgcgg tttcgggctc attgcaaacc
     2161 tggacgacaa gccgagtcac tggctggttg ataccgccgt aacggcactg gccaggttga
     2221 cacaccgggg cgccgttgcg tccgacggca agaccggcga tggctgcggc ctgctgctga
     2281 agtttccagg gcagtttttc cgcagcatcg ctgccgggtg cggcataacc gtcgcggagc
     2341 gctttgccgc aggcacggtt ttcctgagcc aggatgaggc ggtggcgcgt gaggtgaagc
     2401 gcaccatcga ccaggcgctc gaggatgttg gcctcgcggt cgcggggtgg cgggaggtac
     2461 cagtcgatcc ttccgtatgc ggcgtccagg ccctgcagac cttgccacga atcgagcagg
     2521 tcttcgtgaa cgcatcggcc ggcatgcagc acacgtattt caaccgccgc ctctttctcg
     2581 cgcgccgccg cgccgagagc cgcttttcaa aaatcgattc gagcgcgtat atctccagcc
     2641 tctcgtcggc gacgattagc tacaagggca tggtgatgcc agatgtcctt ccggagttct
     2701 acccggacct gaaggacccg cggctcgagg cctccgtatg cgtgttccac cagcgttttt
     2761 cgaccaacac gcttccggag tggcgtcttg cgcagccgtt ccgtttcctt gcgcacaacg
     2821 gcgagatcaa caccatccag ggaaatcgca actgggctgt cgcgcgcgcc gcgaacttcc
     2881 gctcggaaaa gctgcatgac ctttccgacc tggatccgat cgttgcactg aacggctcag
     2941 actcctcgac gctcgacaac atgcttgagg tcctgcgtgc cggcggcatg gacgtgctgc
     3001 aggcgatgcg catcctcatt ccaccggcat ggcagaccgt cgactcgatc gacccggatg
     3061 tccgcgcgtt ctacgagttc tacgactgcc acatggagcc gtgggacggc ccggccggca
     3121 tcgtgctgac cgacgggcac tacgccgcct gctgcctgga ccgcaacggg ctgcgcccgg
     3181 cacgctacgt ggtaacaaaa gaccggcatg tgacgatcgc atcggagacc ggggtgtacg
     3241 actacgaagc cagcgacgtg actgccaagg gcaggctcgg ccctggcgaa atgctcgcgg
     3301 tcgacctgcg gaacgggcgg ctccttacca cgcaggacat ccaccaggac ctgaaggcgc
     3361 gcgcgccata caagaagtgg ctcaagcagg gcgtgaagta cctggactcg gaactggtcg
     3421 accgccacat agcagcggag cccatggatg aggccacgct ggcgacctac cagaagatgt
     3481 tcaatctcac ccgcgaagaa ttgaacgaaa tcgtgagcgt gctggcgaag gcggaacagg
     3541 aagcggtcgg gtccatgggc gacgacacgc cgatgccggt actttcgaaa cagatccgct
     3601 cgctcttcga ttatctccgc cagcagttcg cgcaggtcac gaacccgccc atcgacagct
     3661 tgcgcgaacg caccgtcatg tcgctgcaga cgaacatcgg gcgcgaaatg aacctgttcg
     3721 atatcggacc ggagcacgcg gagcaggtcg tgatgaattc gccggttctg tcgcagcgca
     3781 agctgcgcca gttgctgggg ccggacatgt tcggcggcag tcacgcattc gtcgacctga
     3841 acgcgccgga ggagcaggac cttgccggtg cgctcgaccg catctgcgcg gaatccatgc
     3901 aggcggcgag ggacggaaag ctcgtgatca tgctgagcga ccgttacctg aagaagggca
     3961 tgctgccggt gcacgccctc cttgctaccg gcgcggtgca ccatagcctc gtggccagcg
     4021 gctttcgctg tgcagtcaac atcttagtcg agacgggcgt ggcgcgcgac gcgcaccact
     4081 tcgcgtgtct catcggctac ggcgcgaccg cggtatatcc ctatctcgcc taccagagcc
     4141 ttcacgacct cggccagcgt ggtaacgtgg accgccaccg gcagctggga cgcagctatc
     4201 gtcgtggcat ccgcaagggg ctgttcaaga tcatgtcgaa gatgggcata tcttcgatct
     4261 cgagctatcg cggcgcgcag ctgtttgaaa tcgtcggcct ccacgaagaa gtcgtttcga
     4321 agtgcttcaa gggcacggta agccgcatcc agggagcgaa gttcgccgac ctgcttgaag
     4381 accagcagca gctcggcgca acggcgtggg aaccgcgcct gccgctcaag cagggcggcc
     4441 tgctcaagta cgtgcacgat ggcgagtatc actgcttcaa ccccgacgtc gtcgccacgc
     4501 tgcaggcggc agtgcgctcc ggcgacttcg gacgctacct cgattactcg aagctggtga
     4561 acgagcgtcc ggtttccacc ttgcgcgacc ttttcgcact gcgtcccgca gggccggcga
     4621 tacccctgtc ggaggtcgaa ccggtcagct ccatcctgcc gcgcttcgac agcgccggca
     4681 tgtcgctggg agccctttcg ccggaagcgc acgaggcgct ggcgatcgcg atgaatcgca
     4741 tcggcgcccg ctcgaattcg ggcgaaggcg gcgaagaccc ggcacggcac ggcaccgagc
     4801 gtatgtcgaa gatcaagcag gtcgcgtcgg gcaggttcgg cgttaccgcg gaatacctgg
     4861 tgcacgcgga ggtgatccag ataaagatcg cccagggcgc caagccgggt gagggcggac
     4921 agctgcccgg gcacaaggtc aacgagatga tcgcgcggct gcgctatgcg aaacccggcg
     4981 taggtctgat ttcgccgccc ccgcatcacg acatctactc gatcgaggac ctggcccagc
     5041 tgattttcga cctgaaacag gtgaacccgc aggcactggt gtccgtgaag ctcgttgcgg
     5101 agcccggcgt aggcacgatc gcggcgggcg tcgtcaaagc ctacgccgac ctgatcacca
     5161 tttccggcta cgacggcggc acgggcgcaa gccctttgag ttcggtcaag tacgccggca
     5221 gtccctggga gctcggactg tcggaagcgc accaggtgct ttgcgcgaac gacctgcggc
     5281 agaaggtgcg attgcagacc gacggcgggc tgaagaccgg gatggacgtg atcaaggcgg
     5341 cgctgctcgg cgccgaaagc ttcggcttcg gcacagcgcc gatggtggcg cttggctgca
     5401 agtacctgcg cgtctgtcac ctgaacaact gcgcgaccgg cattgcgacg cagaacaacg
     5461 tattgcgcag cgaattcttc gtcgggctcc cggacatggt ggtgaacttc ttccgtttcg
     5521 tcgcggagga ggtgcggatg cgcctcgccg atctggggct tcggcgcctc gaggacatca
     5581 tcggccgcgt cgatctcatc gagctgaagc cgggagaaac gcggcgccag aagcagctgg
     5641 acctgcggcc gatcgtctca tcgagggggc tcgtgtccga cagtccgcag ttctgcaccg
     5701 acgctcgcaa cgtccccgcg gacaagggcg aactcgccga acgcatggtg aaggagattc
     5761 ttccggcgat agaggcaaag agcggcggca gcttcagcta cggggtgcgg aactacaatc
     5821 ggtcgatcgg cgctcgcctt tcgggcgaga tcgcccgccg atacggtaac cacggcatgc
     5881 aggatgcgcc cgtcgacatt cgccttaagg gcaccgctgg gcaaagcttc ggcgcctgga
     5941 acgtcgcggg cctgaatctc gaactcattg gcgacgccaa cgattacgtc ggcaaaggca
     6001 tggcgggcgg gcgcatcatc gtgaagccgc cctccgatgc cggttacgca gcaaaggaca
     6061 cggtgatcgt gggcaacact tgcctctatg gtgcgacagg agggcagtta ttcgccgcgg
     6121 ggctcgcagg cgagagattc gcggtacgca attccggggc ggttgccgta gtggaaggcg
     6181 ccggcgatca ttgctgcgag tacatgaccg acggcgtggt cgtggtgctg gggagaacag
     6241 gcattaactt tggtgcgggc ctgaccggcg ggttcgccta tgtgctggat ctcgatcgtg
     6301 acttcgtcga tcgttacaac cacgagctga tcgacatcca ccgtgtcact tcggagagca
     6361 tggaagcgca gctgcagcat cttcgcgcga cgatcacgga acacgttgcg atgaccggca
     6421 gcgaatgggg cattgcgatc ctcgacgact tcaggaccta cctgcccagg ttctggctcg
     6481 tgaagccgaa agccgcggaa ctgggatccc tgatccgctc gctgcggcag gctgcctgag
     6541 cggggtcacg agtgtcgaaa catcctttgc agtttctgga agtgccgcgg cgcgacccgg
     6601 agaaacagct cgccgaggcg cgcatcaggc actacgacga gatatacggc tcctacgatg
     6661 gtgcggatgc ggctagccag gcgggacgct gtatcggctg cggcaatccg tattgcgaat
     6721 ggaaatgccc ggtgcacaac tacattcccg attggctcag gctgatcgag gaaggaaagc
     6781 tgttccaggc cgcggagctt tcgcaccaga cgaattcgct gcccgagatc tgcggccgca
     6841 tttgcccgca ggaccgtctt tgcgaaggcg cctgcacgct gaacgacggc atcggcgccg
     6901 tcaacatcgg caacatcgag aagtacataa ccgacgaagc gctgaagctc ggctggcgtc
     6961 cggacatgtc gcatgtgccg gacaccggca agcgggttgc gatcgtaggc gcgggaccgg
     7021 ccggacttgc ggcagccgat gtgctggcgc gcttaggtat aaggtccgtg gtgtacgacg
     7081 cctatcccga gatcggcggc ctcctgacct tcggcattcc gccgttcaag ctcgagaagc
     7141 aggtcgtgca acggcggcgc agcattctcg aaggtatggg aatcgagttt gagctcggca
     7201 cgcgcatcgg cgacgacagg ccgttcggag agttgctcga taatttcgac gcggtgttcc
     7261 tcgcactcgg agcttacact tccgtgagcg gtgacctgcc cggcgagcac ctgccgggcg
     7321 tgcatgacgc gcttccctat ctcgtctcca acgtatgtcg cgaaatgggc atcggaagcg
     7381 gcgttgcaga agcggcgcag ccgtgggtgg atctcgccgg aaagcatgtc gtggtactcg
     7441 gcggcggcga cactggcatg gactgcgtcc ggactgcgat tcgccagggg gcggccagcg
     7501 tcacctgcgc ctaccggcgt gacgaggcaa atatgccggg gtcgcgccgg gaagtggcga
     7561 acagcaagga agaaggagtg cgcttcctgt tcaaccgcca gccggtggag atcatcggcg
     7621 acggccgggc gagcggcgta aagctggtcg agacacgcct cggcgcgccc gatgcgagcg
     7681 gacgccgccg ccccgggacg gtacccggaa ccggggaggt gctgcctgcg gatgcagtta
     7741 ttatcgcgtt cggcttcagg ccgagtccac cggcatggtt cgacgagttc ggcatagaac
     7801 tgctgccttc cggccgggtg agagtcgcga cgataggttc gacgccgttc cagacgacca
     7861 acccgaaagt cttcgcaggc ggcgatatgg tgcgcggctc cgacctcgtg gtgaccgccg
     7921 tctatgaagg ccgcgaagcc gcgaagggta tagcaagcta cctgcacgtc tgagtgccgg
     7981 ttcgccttgc tatttcaggt cgcgtgccgc cggaaggcga acctcaaaaa ttgccccgcc
     8041 atccacatcg cgtgcactaa tcgacccccc gtggccggtg gcgaccagcc tcgcgatgaa
     8101 gaggcccagg ccgaggtgct tacctgtatc gtccgacctt accgagacca gcgaatcgaa
     8161 cagctgggag cgcatgcggc ccggcaacgg cgggccagga ttgctgaccg acagcgtgaa
     8221 tccgctgtgc tccctgtcaa ggcgaatcac gatctcgtcg ccgtcggcgc tgaagccgac
     8281 cgcgttgtcg accagcttgt ccagcatctg gatgatcagt tccggtgagc cctccatcgg
     8341 tgcgctgctg gcgttgctca gatacgtgaa gcggcgcaga ggccatgcgt ctgcatatgc
     8401 agtgacggtg gatgagagca ccgcattcag gtcgaaaact tccgtctccg cctgttcaat
     8461 cagttcttcg acccggcttg cctcgctcat cgcgttgaga atccggtgca ggcgcgcggt
     8521 accttccctg gcgcgctcgg tgtacatcgc cgattccgcg ctcaagggtt catgttccag
     8581 gttctcgaga gacgacgtca cgatcgttag cggtgttcgc atttcgtgag acagcttgga
     8641 cgcgagcgtc cgaaggtatt cgttgtactc tccaagcctc cgtagcacgc tcgagaaact
     8701 tcgcgacagg tctccaatct cgtcactcgc acctgcgctc ggcagctccg gctggacccg
     8761 cttgtcgtca agggccctct cggctgcgct gctcaggcgc cggatgcgaa gcgacagcca
     8821 gctcgcgtag ccgagcagcc cggcagcaac gaggagcgtg gccatcagcg tgaagttcat
     8881 cagccgggtg agagcacggt tcctcaagct caggatggcg tcagttccct gctgcaggat
     8941 gactgctccc gtctgcacgt tccccgacca caccggttgc gcgacggcga cgacggcgcg
     9001 gcttccgtcc gcgttgcgaa accagctgct cgccatctcg cccctcaggg cgtactgcac
     9061 gtattcctgt tgctcgcgtc cgctcgggtc agggtccgca agctctgtct cctccccgga
     9121 ctccagtaac gcgtcgtacg cgatacgcat ccagcccgcg gctattccag gcgccgcatc
     9181 gtcacgcgag gcgaattccc cgacctgcgc gagccgccaa cccaccacgt cgaggacagt
     9241 aagccgcaag tccggctgga cgtaacccgc cgcgacgctc tggagcaacg gcgatacggt
     9301 gaccaggcgg cccggcagat cgccatggaa actccgcgtg cgaaccgctc tcgcgccggc
     9361 gttgtcggtg ttgttgacca ccactcccag gcgaccccgc aggagctggc ggggcacgcg
     9421 cgcctcgagc cggtagcctg cgacagtatc cagccagtat gcggaaatgc gtgtctcgtc
     9481 aggattcccg tccgcgacca gcgttgccac caggttaccc ggcgcctctg gcgtgaaaat
     9541 gtaggtgagc ggcgagatcc cgtcttcggc caccgtcacc agctcgattg aatcggcact
     9601 gcgtccgcgg tcgctgcccg gagtggagta gacgacgttg ccgtcacgca cgtccatgta
     9661 gaggaaaacg gactggcgat acacgccgac ggccagcgat gcggaagctt ccggcaaatc
     9721 gaccagggat tgtggcgcca gtccccagtc atccaggtaa ccatcgacga gcggcgccgt
     9781 cgccagggaa tgaccgtaga gttcgtcgcc tgttttgtcc atcaagtcgc cgcgtcgccc
     9841 ggcattgcct tcgcggggtg caaattcggc cggaaactgc gcaagggagt cggcaatggc
     9901 ctgcgcggta ccggccagca tctgctgctg accctcgcgc agcgcggatt cggtttcgcg
     9961 gacgaactgg caacccgccc atggaagcac cagcgtcagc aggctgacca gcaggagttg
    10021 tcgtctgagg ttcattggca aaggctacta cgtgttccgc aggtcccgca ttcgccgtcc
    10081 gcaaggccgc ccggattcga taccgctctc agatcggttc agcgtgaact cctacggggc
    10141 acggtccggg ctcaccgttc tcccagccag cgatagccca tgccgtaaac cgtctctacc
    10201 gcgtcgaatg ccgaatcgat ggcgaggaat ttccgccgga tccgcttgat gtgtgaagtg
    10261 atggtgttgt cgtcgagcac cgctcgcgcc gcgtccatca attgctggcg gttgcgcacg
    10321 tgccccgggt agcgcgccag cgagtgcacg atccagaatt cggtcagcgt aagtcccacc
    10381 ggctggtcct tccagcgcac cgtcatgcgg tcgacgtcga gttccaggtc cccgcgagtc
    10441 atgcggctgc ttttttcctg cggctgctgc atcgcctcga ggcggcggaa gagcgctgcg
    10501 acccgcgcca tcaggtgcgg gatgctgatg tccttggtca ggtaatcgtc ggcgcccagg
    10561 cgaagtccgg acaccgcgtc aaactcgctg tcgcgtgcgg taaggaagat gatcggcaac
    10621 tcgggagcgc tgctcctgag atcacggcac aactcgaagc cgccttccac ttcgtctttc
    10681 aggttgacgt cgatcaggac caggtcgggg aggcgcgcgg caaaagcgtc catggcggga
    10741 cgccgcgcct catacgtatc gactgaatag ccctcacggc ggaaagccgc ggcgtaattt
    10801 tcccgaatgg ctgcttcgtc ttcgacgatg gcgatgcgct ttgacaagtc ctgctccgcg
    10861 aatgcgacgg cacaatttgc cacatcgggt cggtgcattg ccatgtttgc gccgttgcgc
    10921 cacctcaatc ggctgctgga atggacacat cctgaaaaag gttgcatgcc ggagttcgaa
    10981 cagatgtgcg cgaaaataac aacatggctg gtggaggctg ccttcctggc agcgatgctc
    11041 cttacgcagg cgacggcaag ggcagaaccg gccggtgatg ctttcgcatc gccccacgaa
    11101 atgcagtccg ggagcctgct cctgcggatg gaggcgggct atacgatcgc cacgcggatc
    11161 aacaccgaca tcgagatcca ggtcagcggc ccggttgcgc ggactatcgt cagccaggag
    11221 ttccgcaacg acggagcaaa gtgggtagag ggcgtttatg ttttcccgct gccggacagc
    11281 gccgcggtcg accggctgcg cttgcggatc ggagagcgat tcatagaagg cgaggtgcgc
    11341 gagaaggaag aggcccgggt cgagtacgaa aaggcgaggc aggacgggaa acgcgcaggg
    11401 ctggtcgagc agcagcggag caacctcttt accacgtccg tagccaacct gggtcccggc
    11461 gagacggtca acgtcgagat cgagtatctc gaaaccgtga agtacgacaa tggccagttc
    11521 agccttcgtt ttcccctgac actcaccccg aggtacatcc ccggagtcgg acaagggagg
    11581 cagggttcgg gctggtcggc cgatacccgc caggtccctg acgcatcgat gatcacgccg
    11641 ccggtagtca cggcctcgcg cgaccatgcc gtcacgatgc gcgccgaaat cgatgccggc
    11701 atcccgctcc aggtcatcgc cagccgttat caccctgtca gggtcgaggc tgtggatgag
    11761 gcggaggggc gctatacggt tgcccttaca ggaacgaagg tttcgatgga ccacgacctg
    11821 gaactcgcct ggcggccggt cgaggacgcc gttgcgcgag cgacgctctt tcgcgaagtg
    11881 aaggacggca ggccgcatta cctgctgatg gtgctgccgc cagacgacat ggaactggcc
    11941 agagcggacg ctgccaatcc tgtagcgcgc gagctcgtgt tcgtcatcga tacctccggt
    12001 tccatgcatg gcgcttccat cgaccaggcg aagcaggccc tcctgcttgc attggacgga
    12061 ctggcgcccg gcgacagttt caacgtcatc cagttcaact cgatcgtgga tgtcctttac
    12121 ccccaaagcg tttcggctac gcccggaaat atccggttgg cgagggaata cattcgcaag
    12181 ctggcggcga acgggggcac ggaaatgtac ccggcgctcg agcgcgcgct ggtgccggca
    12241 ggagacgaag cgcggcttcg gcaaatcgtt ttcattactg acggaagcgt gggcaacgag
    12301 caggaattgt tccggctgat agaggcgaag ctccgcagtt cgcggctctt cacggtgggg
    12361 atcggatctg caccgaacag cctgttcatg cgcaaggcgg ccgaatcggg cagaggcgcc
    12421 tataccttta taagcgcgct gcacgaggtg aaggaaaaga tgagtgcgct cgtgcggaag
    12481 atcgagcagc cccgcgtcac cgggatacag gttgcatggc ccggtgaggc gctggcctat
    12541 ccggtggttg ttcccgatct ctacgccggc gaacctgtcg tgcagaaagt gcgcctgacg
    12601 ggagagtcgc gtggcggtga cgtcgtgcgt atatccggca cctcgtccat gggggactgg
    12661 ggtgtcgacc tgccgatcgc ggcggagcag gagaacgagg gcgtcgcggc aatctgggcg
    12721 cgttcgcgca tcgaacacct gctcgatcgg gagcggggag gggaggacgc ggaagccgct
    12781 cgcgcggagg tcatcgaaac cgcgctcgat caccagctcg tca
//
LOCUS       DASUOM010000635         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_211726,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000635 DASUOM010000000
VERSION     DASUOM010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211726"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..297)
                     /gene="atpE"
                     /locus_tag="VFY27_13345"
     CDS             complement(19..297)
                     /gene="atpE"
                     /locus_tag="VFY27_13345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5766545.1"
                     /translation="METVIGFTAISVAILIGLGALGVGIGMGLLGGRFLEGAARQPEL
                     APMLQTKMFLVVALLDAVAMIGVGIALYFAFANPFIGQLQNAMAPVAG"
     gene            complement(330..1169)
                     /gene="atpB"
                     /locus_tag="VFY27_13350"
     CDS             complement(330..1169)
                     /gene="atpB"
                     /locus_tag="VFY27_13350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5766546.1"
                     /translation="MATEGARTTGEYITHHLQNLQVCKADGEWIWNHCAGNPMAVNVD
                     SMFFSVLLGVVFVWLFGTVAKRVSSGRPGKLQAAVEILVEWVDSSVRDTFHGENKVIA
                     PLALTIFVWVFLMNLMDLIPVDWLPFVAENVLHIPYLKIVPTTDVNVTFGMSIAVFVL
                     IVFFTVKNKGFSGFIGELTLHPIAPPTKGLGLIAAPVIIAFNFILESVSLLAKPLSLS
                     LRLFGNMFAGELIFILIAILGIWQLPLHFLWAVFHVLIVTLQAFIFMMLTIVYLSLAS
                     EKH"
     gene            complement(1159..1611)
                     /locus_tag="VFY27_13355"
     CDS             complement(1159..1611)
                     /locus_tag="VFY27_13355"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5766547.1"
                     /translation="MSEAHEKSGASISNPLAVVLLAQTGLTAALAVVFWGWGDVVSGY
                     SALLGGLTAIVPNGFLAARLMLPRRDPGAGALLRAAYLGELGKLVLTALMFGVVFALV
                     RPLAAGALFAGFIVAQLATFAGFLARDRGRDDHGIGDRTGNTDNRNGD"
     gene            complement(1853..2788)
                     /locus_tag="VFY27_13360"
     CDS             complement(1853..2788)
                     /locus_tag="VFY27_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5766548.1"
                     /translation="MTRKKRLGRGLDALLSKPQPEIVQVPSPAFGRPESDRGSAEVGT
                     SDRLVSIPVDLLQRGQYQPRVDMRQESLEDLANSIRSQGVVQPIVARPLQGAGGGTQR
                     YEIVAGERRWRAAQMAGLQEIPAVIRDVPDDQAIAMALIENIQRENLNPLEEARALDR
                     LIREFELTHQEAAEAIGRSRAAVSNLLRLMDLSDKVRPMLEARELEMGHARALLAIED
                     KAQQYDAARQVVRNGMSVRDTERLVRRMLGTETKKRANGSKSAANADVRRLEVEITEK
                     LGARTQIDHTGKGSGRIVIAYNSLDELDGILKHIR"
     gene            complement(2811..>3594)
                     /locus_tag="VFY27_13365"
     CDS             complement(2811..>3594)
                     /locus_tag="VFY27_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5766549.1"
                     /translation="GKTTTCVNLAASLAATHRRVLLVDLDPQGNATMGCGIDKMSLER
                     SACDVLLGEETAADTIVSAPEGDFSVLPGNDDLTLAEVRLLQDIGREMRLRKALAPVR
                     GLYDFILIDCPPSINMLTVNALTAADGVLIPMQCEYYALEGLSSLLNTIEQVRTTVNP
                     ELEVYGLLRTMVDPRINLSNEVSDQLVAHFEDKVFRTVVPRNVRLAEAPSFGRPVLFH
                     DKASRGAMAYLALAGEVLRREDERTTPALQAGNTGDTYHDNY"
BASE COUNT          716 a         1118 c         1067 g          693 t
ORIGIN      
        1 ctttctcttg accctgtttt agcctgctac cggcgccatc gcgttctgca actgtccgat
       61 gaacggattc gcaaacgcaa agtaaagcgc gatgccgaca ccaatcatgg cgacagcgtc
      121 aagcagcgcc acaaccagga acattttcgt ttgcaacatg ggcgccagtt ccggctgcct
      181 tgccgcacct tcgaggaagc gcccgccgag caatcccatc ccgatgccga cgcccagtgc
      241 gccgaggccg atcagaattg cgacggatat cgcagtaaaa ccaataacag tttccatcaa
      301 tgtctcccgt cttttaagtc caataccagt taatgcttct cagatgccag gctcaggtaa
      361 acgatcgtaa gcatcatgaa aatgaacgcc tgtaacgtaa caatcagcac gtggaaaaca
      421 gcccacagga aatgcaacgg gagctgccag ataccgagta ttgcgataag gatgaaaatc
      481 agttcgccgg caaacatgtt gccgaacagt cgcagcgaca gtgaaagcgg cttggcgagg
      541 agagacaccg actcaagaat gaagttgaag gcgatgatca ccggcgcggc gatcaaaccg
      601 aggcccttcg tcggcggcgc gatcggatgc agcgtcagct cgccgatgaa gccgctgaag
      661 cccttgttct tgaccgtgaa gaagacgatc aggacgaaca ccgcgatcga cataccgaac
      721 gtcacgttga cgtcggtcgt cggcacgatt ttgaggtacg ggatatgaag gacgttctcg
      781 gcgacaaacg gcaaccagtc tacggggatc aggtccatca aattcatcag gaaaacccag
      841 acgaagattg tcagcgcgag gggcgcgatc accttgttct cgccatggaa ggtgtcgcgt
      901 acactcgagt cgacccattc gaccaggatt tcgactgccg cctggagctt tccggggcgc
      961 cccgaagaca cccttttcgc gaccgtacca aacaaccaga cgaagacgac tccgagcagc
     1021 accgagaaaa acatcgagtc cacgttcacg gccatgggat tgccggcgca atgattccag
     1081 atccactcgc cgtcggcctt gcacacctgc aggttctgca ggtgatgggt tatatattcg
     1141 ccggtcgtcc gggcgccttc agtcgccatt cctgttatcc gtatttcctg tcctgtcacc
     1201 tatcccatgg tcgtcccggc ccctgtcgcg tgccaaaaag ccggcaaacg tcgccagttg
     1261 tgccacgatg aagcctgcga acaatgctcc tgccgcaagc ggccgcacca gcgcgaagac
     1321 cacaccaaac atgagggcgg tcaataccag ctttcccagt tccccaagat aagcggcccg
     1381 gagcaaggca ccggctccgg gatcgcgacg cggcaacatg agccttgccg ccagaaaacc
     1441 gttcgggact atcgctgtca ggccgccgag caaggcggag tatccggaca cgacgtcgcc
     1501 ccaaccccag aacaccacgg cgagcgccgc cgtcaatcct gtctgtgcca gcagcaccac
     1561 cgctaacggg ttgctgattg aggctccgga tttctcgtga gcctcgctca tcgtgcgtcg
     1621 cttgcctcgc gcaacaaaaa cgccacgacc cgcggctaac cggcggtagg tggcgttctg
     1681 attaaagtat tcccgggttg aatggtcccg agggggcttt cgctgagccc gcgcattata
     1741 gtgtcctgca tggggcaagg caaccaaggg aagcaccgaa aaaacctcgc atccgcacag
     1801 gcttgatgag ggttcggggc gtaatttcaa cgtcgattgt tgggcgacgt agctaccgga
     1861 tgtgtttcag gatgccgtcc agttcgtcga ggctgttgta ggcgatgaca atgcggcccg
     1921 aacccttgcc tgtgtggtcg atctgggtcc tcgcgccgag cttttcggtg atttcgactt
     1981 cgaggcgccg cacgtcggcg ttcgcggccg atttggagcc gtttgccctt ttcttcgttt
     2041 cggttccgag catgcgacgg accaggcgct cggtgtcccg gacggacatg ccgttcctga
     2101 cgacctggcg cgcggcatcg tactgctgcg ccttgtcttc gatggcgagc agggcacgag
     2161 cgtgtcccat ctccaactcg cgcgcttcga gcatcggcct gaccttgtcg gacagatcca
     2221 tcaggcgcag caggttgctg acggcggcgc gggagcggcc aatggcttcg gcagcctcct
     2281 ggtgcgtcag ctcgaattcc cggatcagcc ggtccagagc gcgcgcctcc tccaggggat
     2341 taaggttctc gcgctgtata ttctcgatca gcgccatcgc gatggcctgg tcgtccggca
     2401 cgtcgcggat gaccgccggg atctcctgca ggcctgccat ctgcgcggcg cgccagcggc
     2461 gctcgccggc aacaatctcg taacgctgcg tgccaccgcc tgcgccctgc agcggacgcg
     2521 cgacaattgg ttgcacgaca ccctgcgagc ggatcgaatt cgccaggtct tccagcgact
     2581 cctgtcgcat gtcgacgcgt ggctggtact ggccgcgctg cagcaggtcg acaggaatgc
     2641 ttacgagcct gtcgcttgtc ccgacctcgg cgctaccccg gtcgctttcc gggcggccga
     2701 atgccgggga cggcacttgt acgatttctg gctgtggctt gctcaggaga gcgtcgaggc
     2761 ctctgccgag gcgcttcttt ctggtcactg gaacatcccg ggctgctgcg tcagtagttg
     2821 tcatgatagg tgtcgcctgt gttgccggcc tgcagggccg gggtggtgcg ttcgtcttcg
     2881 cgtcgcaaaa cttcacccgc cagggcgaga tacgccatcg cgccgcgcga tgccttgtca
     2941 tgaaagagaa ccgggcggcc gaaactcggt gcttctgcaa gccgcacgtt ccgcggtacg
     3001 accgtgcgga ataccttgtc ttcgaaatgc gccacgagct gatccgagac ctcgttcgac
     3061 aggttgatcc gcgggtccac catcgtgcgc agcaagccat agacctcgag ctccggattg
     3121 actgttgtcc ggacctgctc gatggtgttc aggagagagc tcaagccctc gagagcgtag
     3181 tactcgcatt gcatcgggat cagcacgcca tcggccgcgg tcaacgcgtt gaccgtcagc
     3241 atgttgatgg acggcgggca gtcgatgagt atgaagtcgt agagtccccg taccggcgca
     3301 agcgccttgc gcaggcgcat ctccctgccg atgtcctgca gcaggcgcac ctcggcaagc
     3361 gtcaggtcgt cattgcccgg caatacggaa aaatcgcctt ccggcgccga gacgatggta
     3421 tccgccgccg tttcctcgcc gaggaggaca tcgcacgcgg accgttcgag gctcatcttg
     3481 tcgatgccgc agcccatcgt cgcgttaccc tgcggatcga ggtccacgag aagcacgcgc
     3541 cggtgagtcg ccgccagcga agcggcaaga ttgacgcagg tggtggtttt accg
//
LOCUS       DASUOM010000636         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1296591,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000636 DASUOM010000000
VERSION     DASUOM010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296591"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="moeB"
                     /locus_tag="VFY27_13370"
     CDS             complement(<1..904)
                     /gene="moeB"
                     /locus_tag="VFY27_13370"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX5766550.1"
                     /translation="MTADTSTASQATIEETSVDSRLPELSPDEILRYSRHLIIPDVAL
                     GGQRRLKGSRVLLVGAGGLGSPLALYLAAAGVGTLGLVDFDVVDLTNLQRQIVHGTKD
                     VGRSKIASAEERIADVNPHVRVEPYETQLTSVNALEIMRDYDVIVDGTDNFPTRYLTN
                     DACVLLGKPNVYGSIFRFEGQASVFATADGPCYRCLFREPPPPGLVPSCAEGGVLGVL
                     PGIIGTIQATETLKLLLGIGEPLIGRLLLFDALRMQVRTMQLRRDPACPACGTHEIRE
                     LIDYEEFCGIRQAAVAEAGNPVPEI"
     gene            complement(1194..1499)
                     /locus_tag="VFY27_13375"
     CDS             complement(1194..1499)
                     /locus_tag="VFY27_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766551.1"
                     /translation="MSEERYLRYLRTEKNETGPGTAVLPDNLRNLRNLWFAVVRNGAA
                     LRLIRASLATAVALFERRQKKETADFADFADCMNHHRDLRYAVRKEKSGLVSAGALG"
     gene            complement(1558..2565)
                     /gene="thrB"
                     /locus_tag="VFY27_13380"
     CDS             complement(1558..2565)
                     /gene="thrB"
                     /locus_tag="VFY27_13380"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5766552.1"
                     /translation="MSAGVKVRVPGSTSNLGAGFDCVGIAVDRWLSLSAVVEADSGEP
                     VRLHRDGTLSGLTIAPGEDRIVAAFTAACDAAGRQVPHGLRMRASSDIPVARGLGSSA
                     AAVVAGAAAANELLRLGFDDQRLLELCASIEGHPDNVVPAIQGGATLAIAASPGHELH
                     AGFPAWVTARIDLHPDLALALAVPDFEAETEFMRAALPATVPHAVATRAAALGAALVS
                     GLTSGDGRLLELALDDVLHVPFRRGNVRGYDEVVQAARSAGAFGATLSGSGSTICGLT
                     PRSAVHDVAAAMVKAWRGLDVQAEAIVCETPAGGYSATAGDEYVNDFNAGTGGAARNN
                     P"
BASE COUNT          510 a          906 c          790 g          496 t
ORIGIN      
        1 cgatttccgg gactgggttt ccggcctcgg ccacggccgc ctgacggatt ccacaaaact
       61 cttcgtagtc gatgagctcc cggatctcgt gcgttccgca cgcggggcaa gccggatccc
      121 ttcgcagctg catcgttcgc acctgcattc gcaaggcatc gaacagcagc aggcgcccga
      181 tcagcggctc gccgattccg agcagcagct tgagggtttc cgtcgcctgg atggttccga
      241 tgatacccgg cagcaccccg agcaccccac cttcggcgca gctgggcaca agccccggag
      301 gcggaggctc cctgaacaga caacggtagc acggcccgtc ggcggtcgcg aacacagatg
      361 cctgcccctc gaaacgaaag atgctgccat agacattcgg cttgccgagc aggacgcaag
      421 catcgttcgt caggtagcgg gtggggaagt tgtcggtgcc gtcgacgatc acatcatagt
      481 cgcgcatgat ctccagcgcg ttcaccgacg tgagttgtgt ctcgtaaggt tcgacccgga
      541 catggggatt gacgtcggcg atgcgctcct ctgccgaggc gatcttggag cggccgacgt
      601 ccttagtacc gtgcactatc tggcgctgga gattcgtcag gtccaccaca tcgaagtcga
      661 ccaggcccag tgtcccgacg ccggcagccg ccaggtagag tgccagcggc gagcccaatc
      721 ctcccgcccc gacgagcagg actcgcgatc ccttgagccg gcgctggccc ccaagcgcga
      781 catcggggat gataaggtgc ctgctatagc gcagtatctc gtcaggcgac agttcgggca
      841 gtcgggaatc aactgatgtc tcctcgatcg tggcctgcga agcggtactg gtatctgccg
      901 tcatgtcatc cccctgctat tgccggaacg atggtgctca tcaccgtcct gtactggtgc
      961 atcgaaaccg ccccgaaatc caacatcctc atcattcagg tagaacgtca cgagtgcgca
     1021 cggtgttccg gatcaatcac ggaccgtggc gctcgcgtgg aataacgggc ggcaacgtcg
     1081 ccgatcacct caccgtcggt agctccgtta actctcagtg ccgccgccgg acaactttgc
     1141 gaccacgtct gcaagtacag gcacgcgatg ccgttcattt ccaacaccgc agatcatcca
     1201 agagcaccgg cactcacaag tccgctcttt tcttttctta ctgcataccg aagatcacga
     1261 tggtggttca tacaatctgc gaaatctgcg aaatctgcgg tttccttttt ctgtcgtcgt
     1321 tcgaatagcg caacggccgt cgccagcgac gcacgaatca gccgcaaagc cgctccgttt
     1381 cgaacaacag caaaccacag attacgcaga ttacgcagat tatccggaag cacggcagtt
     1441 ccaggtccgg tttcattttt ttccgttctg agataccgga gataacgttc ttcgctcatg
     1501 caatccgcga aatctgcggt tgcatttgtc gtcccccgaa tggttcatct atggcgttca
     1561 agggttgttg cgcgccgcgc cacctgtccc ggcattgaaa tcgttgacat actcatcgcc
     1621 ggccgttgca ctgtatccgc ccgccggagt ttcgcatacg atcgcctcgg cctgcacatc
     1681 aagtccgcgc catgccttca ccatcgctgc cgccacatcg tgtacggcag agcgcggcgt
     1741 caagccgcag atcgtcgagc ccgatccgct gagcgttgcg ccaaacgcac cggccgaccg
     1801 cgccgcctgc accacttcgt cgtagccccg cacgttgcca cggcggaagg gaacgtgaag
     1861 cacatcatcg agggcgagct cgagtagtcg gccgtctccg ctcgtcaggc ccgatacgag
     1921 cgccgcgcca agcgccgccg cgcgcgtggc aaccgcgtgc ggcaccgttg ccggcaaggc
     1981 tgcacgcatg aactccgtct cggcttcgaa gtcgggcacc gccagcgcaa gcgcgaggtc
     2041 cggatgcagg tcgatgcgcg ccgtaaccca tgccgggaat ccggcgtgca gctcatgccc
     2101 cgggctggca gcgattgcaa gggtggcacc gccctgtatt gccggcacta cgttgtcggg
     2161 atgaccttcg attgaagcgc acagctccag cagacgctgg tcgtcgaatc caagccgcag
     2221 aagttcattg gcggcggcgg cgccggccac gaccgccgcc gccgaggagc ccagcccacg
     2281 cgcgacggga atgtcggaac tggcgcgcat gcgcagcccg tgcggaacct ggcgaccggc
     2341 tgcatcgcaa gcagcagtaa acgccgcgac gatacggtcc tcgcctggcg cgatcgttag
     2401 cccactcagc gtaccgtcgc ggtgaaggcg aaccggttcg ccgctatcag cttccacaac
     2461 cgcgctgagg gagagccacc ggtccaccgc gatacctacg cagtcgaagc ctgcacccag
     2521 attggatgtg gagccgggaa cgcgaacttt gactccggca ctcatcgcgt tctgcgcggc
     2581 gttctgcgcg ccgggacggt ccggctcacg cgccgcctca caatcagtcc ggtgatcgcc
     2641 gaatggtact ccgggaatgt catacgcgtc accgctgccg ctacgtccgg ctccctatcg
     2701 cc
//
LOCUS       DASUOM010000637         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1782141,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000637 DASUOM010000000
VERSION     DASUOM010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782141"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFY27_13385"
     CDS             <1..901
                     /locus_tag="VFY27_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5766553.1"
                     /translation="VPVDWWAVIFNPSFPYRFVHMVLAAYLATAFIVGGVAAHHLLRN
                     STHRDSRVMFSMAMWMATLVAPLQIFVGDLHGLNTAEHQPMKVAAMEAHFETRRGQPL
                     ILLGIPDMDAGSVRYALEIPNGGSILLGHAADAEVPGLDRLQREDWPHVPTMFFSFRI
                     MVGLGLIMAGIGLWSLVQRLRGRLFETGSLLCMARLFAPAGIVALLCGWVVTEVGRQP
                     YTVYGLLRTTESVSPIALPAVAGSLAVFIVVYTAVFGVGVFYLLRLMRQDPAAGAVDE
                     ELEEERERTSMLRRLTAWVREEP"
     gene            898..1908
                     /gene="cydB"
                     /locus_tag="VFY27_13390"
     CDS             898..1908
                     /gene="cydB"
                     /locus_tag="VFY27_13390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5766554.1"
                     /translation="MMGSLDLAMIWVILLAIGVLAYVLLDGFDLGVGILFPFLGDESD
                     QTTAMNSVAPVWDGNETWLVLGGGGLFAVFPLAYAVIMPALYAPIIAMLMGLILRGVA
                     FEFRGSTDRRRYLWDRSFFLGSLAATLCQGIILGALVQGIPMENRAYAGGWWDWLTPF
                     SLVTGIALVAGYCLLGATWLVLKTEGELQRRARRMAGIFGWATLAGIGTFSLWTPFLQ
                     AGYMEKWFSWPALLYTAPVPLLVLAVTYTLFRTLKAGKDMAPFLCAEALFVLSFIGLW
                     ISFYPYIVPSSVTIWDAAGPDVSLGFLLVGALVLLPLILAYTAGVYWVFRGKVRPGEG
                     YH"
     gene            complement(1835..>3044)
                     /locus_tag="VFY27_13395"
     CDS             complement(1835..>3044)
                     /locus_tag="VFY27_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5766555.1"
                     /translation="TDKERTKGPRTIKVLILTPTRELAAQVEQSVIDSAKYLSIRSSA
                     IYGGVSIRPQIAELRKGVDIVVATPGRLLDHASQRTIDLSHVEVLVLDEADRMLDMGF
                     IRDIKKIIAMLPKTRQNLLFSATFNKETTDLANRLLQSPAKIEVAGAHRTADLVSQRI
                     YPVDRARKRELLSLLIGSENWRQVLVFTRTKHAANRLATQLEKDGLNASAIHGNKSQG
                     ARTRALREFKNGQVRVLVATDIAARGLDIDQLPHVVNFELPQVAEDYVHRVGRTGRAG
                     KEGEAVSLVCGEEQALLAGIEKLLRRSIPRTVVPGFETGSRATAEDVRRAPARRNENG
                     QGKEKAKRMKYASAPTAATPDNQKSTNQPSRRKRRNRGGPASSGVNDSPRRGAPSRGT
                     PSTHQRYTQG"
BASE COUNT          442 a          928 c          991 g          683 t
ORIGIN      
        1 cgtgcctgtt gactggtggg cggtcatctt caatccctcc tttccatacc gttttgtgca
       61 tatggtgctg gccgcctacc tcgctaccgc gttcattgtc ggcggcgtcg cggcccacca
      121 tcttctgcgt aacagtacgc atcgcgactc gcgcgtcatg ttttccatgg cgatgtggat
      181 ggcgaccctg gtggccccgc tgcagatttt cgtcggcgac ctccatgggt tgaacacggc
      241 ggaacaccag ccaatgaagg tcgcggccat ggaggcgcac ttcgaaaccc gccggggcca
      301 accgctgatc ctgcttggca tccccgacat ggacgcaggg tcagtgcgct acgcgctgga
      361 aatccccaac ggcggcagca ttctgctggg acacgccgcc gacgccgagg tgccgggcct
      421 agaccgcctc cagcgcgaag actggccgca cgtgcctacg atgttcttca gctttcgcat
      481 catggtggga ctcgggctga tcatggccgg cataggactg tggagcctgg tgcagcgcct
      541 gcgggggcgc ctcttcgaga ccggcagcct gctctgcatg gcgcggttgt tcgcgccggc
      601 gggaatcgtg gcactgctat gtggctgggt cgtaacggag gtcggcagac agccttatac
      661 cgtctatgga ttgctgcgaa ccacggaatc cgtatcgccc atcgcattgc cggctgtcgc
      721 gggatcgctt gcggtgttca tcgtcgtcta cacggccgtt ttcggtgtcg gcgtgttcta
      781 cctgttgcgg ctcatgcgcc aggatcctgc cgccggtgcg gtggatgagg aattggagga
      841 agagcgcgag cgcaccagca tgctgcggcg actcacagcc tgggtacgtg aggaaccttg
      901 atgggctccc tggatctggc aatgatctgg gtgatcctgc tggcgatcgg cgtgctcgcc
      961 tacgtgctgc tggacggttt cgatcttggc gtagggatcc tgtttccgtt cctcggggat
     1021 gagtccgacc agacgacggc gatgaactcc gtcgctcccg tctgggacgg caacgaaacc
     1081 tggctcgtgc tcggcggcgg cggcctgttt gctgttttcc cgctggctta tgcagtcatc
     1141 atgcccgcgc tctatgcccc gatcatcgcg atgttgatgg ggctcatcct gcgaggtgtc
     1201 gcgttcgagt tccgcggcag taccgatcgg cggcggtact tgtgggaccg ctctttcttc
     1261 cttggttcgc tggcagccac gctctgccag ggcatcatcc tgggtgcgct ggtgcagggc
     1321 atcccgatgg agaaccgcgc ttatgccggc ggctggtggg actggctgac gccgttcagc
     1381 ctcgttaccg gcattgctct cgtcgctggt tactgcctgc tgggtgcaac ctggctggtg
     1441 ttgaaaaccg agggcgaact gcagcggcgc gcacggcgga tggcgggcat ttttggctgg
     1501 gcgacgctcg ccgggatcgg caccttcagc ctctggacgc ccttcctgca ggcaggttac
     1561 atggagaaat ggttcagctg gccggcgctc ctttatacgg cgccggtgcc gctgctggtg
     1621 ctcgcggtga cgtacacgct gttccggacc ctcaaagcag gcaaggacat ggcgcctttc
     1681 ctgtgcgcgg aagccctgtt cgtgctgagt ttcatcgggc tctggataag cttctatccc
     1741 tacatcgtgc cctcgtcggt cacgatctgg gacgcagcag gtccggatgt gagtctcggg
     1801 ttcctgctgg tcggtgcgct cgtcctgctc ccccttatcc ttgcgtatac cgctggtgtg
     1861 tactgggtgt tccgcgggaa ggtgcgcccc ggcgagggct atcattgaca cctgatgaag
     1921 cggggccgcc gcggttccgg cgcttgcgcc ggctcggctg gttcgtgctc ttctggttgt
     1981 caggcgttgc ggcggtcggg gcgctggcgt acttcatacg cttcgctttt tccttcccct
     2041 gaccgttttc gttgcgccgg gccggggcac ggcggacatc ctcagcggtt gcgcgcgatc
     2101 cggtttcgaa gcccggtacc acggtacggg gaatgcttcg cctgagcagc ttttcgatgc
     2161 cggccagcag tgcctgttcc tcgccgcaga cgagggaaac ggcttcgcct tccttgcccg
     2221 cgcggccggt gcggccaacc cggtgcacgt aatcctcggc aacctgcggc aattcaaaat
     2281 tgacgacgtg cggaagctgg tcgatgtcga ggccgcgcgc ggcgatatct gtcgcaacca
     2341 gcacgcggac ctggccgttc ttgaactccc gcagcgcgcg agtccgggct ccctggctct
     2401 tgttgccatg gatggctgac gcgttcagtc cgtctttctc gagctgcgtt gcgagtcggt
     2461 tcgctgcatg cttggtgcga gtgaatacga gcacctgtcg ccagttctca gagccgatca
     2521 gtaaggacag cagctcgcgc ttccgcgcgc ggtcgaccgg gtatatccgc tgggacacca
     2581 ggtccgccgt cctgtgtgcg ccggcaactt cgatcttcgc cggggactgc agcaggcggt
     2641 tcgcgaggtc cgtggtttcc ttgttgaacg tcgccgagaa cagcaggttc tgcctggttt
     2701 tcggcagcat cgcgatgatt ttcttgatat cgcggataaa tcccatgtcg agcatgcggt
     2761 ccgcttcatc gagcacgaga acttccacgt gagagagatc gatcgtgcgc tgcgatgcgt
     2821 gatccaggag gcggccgggc gtggcaacga cgatatcgac acctttgcgc aactctgcta
     2881 tctgcgggcg aatggagacg ccgccataaa tagcggaaga cctgatcgac aggtacttcg
     2941 cactgtcgat gacgctttgc tcgacctgcg ctgccagctc acgggtcggt gtgagtatca
     3001 gcaccttgat cgtccgaggt cccttggtcc gttccttgtc agtg
//
LOCUS       DASUOM010000638         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1543750,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000638 DASUOM010000000
VERSION     DASUOM010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543750"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFY27_13400"
     CDS             complement(<1..333)
                     /locus_tag="VFY27_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5766556.1"
                     /translation="MLLTIEYFLLAAAGLLLLSVIASKAFGRMGIPALLLFLGIGMLA
                     GSDGPGGIHFDNPWLAQSLGVVALTFILFAGGMDTEWASVRRVLGIGISLSTVGVAVT
                     AGLVGWFAT"
     gene            complement(340..1218)
                     /locus_tag="VFY27_13405"
     CDS             complement(340..1218)
                     /locus_tag="VFY27_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766557.1"
                     /translation="MTHAGVRQGARVIVLPTDFQRPARRAFLHSVRLAIAFKARLHIL
                     HVIKTPTDRSGEARDSRFLRSLRTSALLELGRLVRTARDGGASGQPLLLYGDPVSSIL
                     EGVKRTHAGMIVMGTEGRIGWDRLRIGSTAEALVREAPCPVLTVHGGLAGDVARHSAR
                     VHLQRVLMATDFSRPADAALRTASGVALNLGATFRLVHVVGPSSEKSRAERKIAAQVH
                     DLRQRGLEAEGQCLDGEPVQAILRDAGVWHADLLALGTSGRRGLHRLALGSVAEELLR
                     RSGCPVLTVRKIHLQW"
     gene            complement(1326..1523)
                     /locus_tag="VFY27_13410"
                     /pseudo
     CDS             complement(1326..1523)
                     /locus_tag="VFY27_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
     gene            complement(1923..2087)
                     /locus_tag="VFY27_13415"
     CDS             complement(1923..2087)
                     /locus_tag="VFY27_13415"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5766558.1"
                     /translation="MAGIGFTMSLFIEGLAFGKTSLDAPAKVGILTASTVAGTLGWLL
                     LKRSRQAKEE"
BASE COUNT          427 a          695 c          639 g          338 t
ORIGIN      
        1 ggtggcgaac cacccgacca gcccggcggt aacggcgacc ccgacggtgg acaaactgat
       61 gccgatgccc aggacccgcc gcacactggc ccactctgtg tccatcccgc ccgcaaagag
      121 aatgaaggtc agggcgacca cgccgaggga ttgagccaac cagggattgt cgaagtgaat
      181 gcctccggga ccatcggatc cagccagcat tccgatacca agaaacagga gcagcgcggg
      241 gatacccatg cgtccgaacg ccttgctggc gatcacgctg agcagtaaga ggcctgcggc
      301 ggcgagcagg aaatattcaa tggtcaggag catggcgcgt caccactgca ggtgtatttt
      361 ccggaccgtg aggacggggc accccgatcg tctcagcaac tcctcggcta cgctgccgag
      421 cgcgagacgg tgcaggcccc gtcgcccgct cgtaccgaga gccaggagat cggcatgcca
      481 aacgccggcg tccctcagaa tggcctggac cggctctcca tcgagacact ggccttccgc
      541 ttccagccca cgttgtcgaa gatcgtgcac ctgggcggca atcttccgct ccgcacggga
      601 tttttccgac gacggaccga ccacatgcac cagtcggaac gtcgcgccga ggttcaacgc
      661 cacaccggag gcggtccgga gcgccgcgtc ggcggggcgt gaaaaatccg ttgccatgag
      721 cacgcgctgg agatgcactc gtgcggaatg tcgggcgacg tcgcccgcca ggccgccgtg
      781 gacggtcagc acggggcagg gcgcttcccg aaccaacgcc tccgccgtgc tgccgatccg
      841 cagccgatcc caaccgatgc gtccttcggt gcccatcacg atcatgccgg cgtgcgtccg
      901 tttgacgccc tccagaatgg aggagaccgg atcgccataa agcaacagcg gctgcccgga
      961 tgcaccgccg tctcgagccg tgcgtaccag acggccaagc tccaacaggg ccgaggttct
     1021 gagcgaacgg agaaaccggc tgtcccgcgc ttcgcctgag cgatccgtcg gggtcttgat
     1081 gacatgcaga atgtgaagcc gtgccttgaa ggctatggca agcctcacgc tatggagaaa
     1141 cgcccggcga gccgggcgct ggaaatcagt aggcaggaca atgacccgtg ccccttggcg
     1201 aacaccggca tgtgtcataa gttcctcata gagttcttca ccatcctaga cgcccgacaa
     1261 tagagaagta ttgcgggcga atacaagttc cgacagtgaa gagcgcctgc cagtgcgcgc
     1321 ccctgctagc ggctcagcgt cggctcgcct cgcacggcca attgcccctg ccgggcataa
     1381 tcgatcgcct cgccgagaat ccgcgccgct tcctgcggcg cgtggaaccc catcgaattc
     1441 tccgcctcga cgaaatccac catgaactgt gccttgcgct gaagcccgcg tgccggcgcg
     1501 agagcgtcgt cgcttcggcc gcctttgcgg gcggcggcga gagtttaagg attggacaca
     1561 cggcgagacg ggagcgtccg tgctcaaggc gcagcatccc ggagttcgaa atgtggaacc
     1621 agggcatcca tgcccgttcg ggcatcgcct gcgccgacgc cacgtagatg agcacccacc
     1681 tggtcgtggg gtcaacgccc aggcttgtcg agcggtgaag cctcgattgc agcgctgatg
     1741 aataaagcgc cctaggattc gctccgatac atgtaggaga cgctccagat gaaaccatgt
     1801 tccttcatcg agggacgtcg actgcacaca agcccgacgc aggccgcgtt cagtgaatga
     1861 agccgtcgac cgaggtaaca tcgacacacg gcctcctcga tcttggattg catcatagcg
     1921 gctcactcct cctttgcctg tcgactgcgt ttcagcagca gccaaccgag ggtccccgcc
     1981 accgtcgagg cagtcaggat acccactttc gcaggggcat cgagcgacgt cttcccgaat
     2041 gccagccctt cgatgaacag cgacatcgtg aagccgatgc cggccaggaa tcccgtccc
//
LOCUS       DASUOM010000639         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_578335,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000639 DASUOM010000000
VERSION     DASUOM010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578335"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VFY27_13420"
     CDS             <1..1936
                     /locus_tag="VFY27_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766559.1"
                     /translation="LLIRAAVTPDDAASPMSLSVAVLPFADMSPGKDSEYLADGMAEE
                     ILNALSGSTSLPVIARTSSFSFKNRNDDIATIARQLNVSHVVEGSVRRSGDRIRVTVQ
                     LILAADGTRVWSAAYDRRLVDVLRLQDEIARSVSAALEATLLGPVTAAQARQPVDPKA
                     YDLYLRGRQGLRDVQLRDAERYLEQSLAIDPRFVPAYVALGEAYVRRILDIDVPIGPY
                     REKLRGLLERGREYAPDDAGLIGLSGQLARYDGDIVLAEERFRQALELDPSNGTVQMV
                     YVMLKGDQGYPEQVLDIAGHAREIDPLNPLIYLGDWSAAMDMGKAQDALKATERYAKL
                     AAPANTAALGLRSMTKMVLLGDLAGGARDWAAAMDIYSSGATRPYADPFWYYVLGDLE
                     NGDAAREIYDRLVVQYPADDFAVVYRHLVTGEIARARELALPLFIGRGDYSALYEDAL
                     IARLAVDALIDRGDANLAIDIIEDLAPQYATFRAVKQIAPERLSPAPYPVKSFYSSYP
                     ALYFPDYIRALRAAGDAGDAGNMLNHLEAILRWRRARGLLVEERHVAEARALGGDYDG
                     ALTALEQALKDRTIFHGWHVFLLHNPIFADIRERPRFMEIVEQVRDEVQMQRAEFTGE
                     SAGEDASDPGLRRPDLAGQD"
     gene            complement(2116..3144)
                     /locus_tag="VFY27_13425"
     CDS             complement(2116..3144)
                     /locus_tag="VFY27_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766560.1"
                     /translation="MNGRLSRKPLIVVLASTALLASVEAHAAKPVDSSGLPFGNGFPS
                     GAHFNLNLSAKADHFTCPEPSFDELGNQEFGKVIFFPRVQGEDPITVLMESGTKGPKG
                     APDTETLEVTDWCTESFPDFGQASGDSAVLRLPYNANGYAVYGTLGGKPLKDGEPTVS
                     ISPDLFYVEDEAGNDLVLLGLVDSSGTATFGSDGTILYRTDPSGSTKGKGVRKATDLT
                     GLFEYSGEICYVQSDPSAYCYDESGSYTCTTRDLCCVDDNSDGVYEACDLLTNVAVLV
                     DDGMGGTLLTCPSDPTVDPYLPIAVSAACQSYENEFVFNIADFVGYLWNIDSTGAYLI
                     RIRFYPIP"
BASE COUNT          625 a          947 c         1000 g          608 t
ORIGIN      
        1 attgctgata cgggccgctg tgacgcccga tgatgccgcc tcgccgatgt cgctctccgt
       61 cgccgtattg ccgttcgcgg acatgagccc cgggaaggat agtgaatacc tggccgatgg
      121 catggcggag gagattctca atgcgctgtc cggctcgacg tcgctgccgg tcatcgccag
      181 aacctcgtca ttttcgttca agaaccggaa cgacgatatc gcgaccatcg ccaggcagct
      241 caacgtttct cacgttgtcg agggcagtgt ccgaaggtcg ggagatcgaa ttcgagtgac
      301 tgtacagctc atcctggcgg ccgacggaac ccgcgtctgg tcggcagcgt atgacagacg
      361 cctcgtcgac gtcctacggc tgcaggacga aatcgcaagg tccgtttcag ccgcactcga
      421 ggcaacactg ctcgggccgg taacagctgc gcaggcgcgg cagccagtgg acccaaaagc
      481 ctatgacctc tacctccgcg gccggcaagg actgcgtgac gttcaacttc gcgatgccga
      541 acggtatctg gagcaatcgc tcgcgatcga cccgcggttc gttcccgcgt acgtagctct
      601 cggcgaggcg tacgtccgac gaatactcga tatcgatgtc ccgatcgggc cataccggga
      661 aaagctgcgt ggtctgctgg agcggggccg ggaatacgcc ccggacgacg ccgggctgat
      721 cggcttgagc ggccagcttg cgcgttacga cggcgatatc gtcctggccg aggagcgatt
      781 tcgccaggcg ctcgagcttg acccgtctaa cgggaccgtg caaatggtct atgtcatgtt
      841 gaagggagac cagggttatc cggagcaagt actggacatc gctggccacg ctcgtgagat
      901 cgatccgctg aatccgctca tctatctcgg cgactggtct gccgccatgg acatgggcaa
      961 agcgcaggac gcactcaagg ccacagagcg ttacgcgaag ctggcagcgc cggccaatac
     1021 cgcggctctc ggcctgcgct cgatgaccaa gatggtcttg ctgggcgatc tggccggcgg
     1081 tgcccgggac tgggcggctg ctatggacat ctatagcagc ggagccacgc ggccttatgc
     1141 cgatcccttt tggtactacg ttctgggcga tctggagaat ggagacgccg ctcgcgaaat
     1201 ttatgatcgg cttgtcgtgc agtatcccgc cgatgacttc gctgtagtct atcggcatct
     1261 cgtaaccggc gagatcgcca gggcccggga actggccctt ccactcttta tcgggcgtgg
     1321 agactactcg gcgctctacg aggatgcgct catcgcgcgt ctcgccgtcg atgcactgat
     1381 cgatcgcggc gacgcgaatc tcgcgatcga catcatcgaa gatttagccc cgcaatatgc
     1441 aacctttcgc gcagtgaaac agatagcgcc cgaaagactt tcacccgccc catacccggt
     1501 caagagtttc tactcctcat atccagcgct gtacttcccc gactacattc gagctttgcg
     1561 ggccgccggc gacgcgggcg atgccgggaa catgctgaat cacctggaag ccatcctgcg
     1621 atggcgccgg gcaagaggtc tgcttgtcga ggaacgccat gtcgcggagg ctcgcgcgct
     1681 aggtggcgat tacgatggcg cgctgacggc tctcgagcag gcgctaaaag accgcacgat
     1741 cttccacggg tggcatgtct tcctccttca caacccgatt ttcgccgaca tccgcgagcg
     1801 tccgcgattc atggagatcg tcgagcaggt gcgggacgaa gtacagatgc aacgggcaga
     1861 gttcacaggc gaatcagctg gggaagacgc ttcagaccct ggtctacgcc ggcccgatct
     1921 tgctggacaa gattaatcta cacgagcttc aagccggttg tacgaccaat gcccctgtgg
     1981 gagcagccct gacccgtacg atgtcaattt cacgcgcgca gccggaggta gcagtggcgg
     2041 caacggcgct gcgtggccga aagatgttcg tcacccggac cgcgcaagcg attcgggcga
     2101 cgaacaaggg ggatatcagg gaatgggata aaaccggatg cgaatgaggt aagccccggt
     2161 cgaatcgata ttccacaggt acccgacgaa gtcggcgatg ttgaagacga actcgttctc
     2221 gtaggactga caggcggcgg agacggcgat cggcagatat ggatccacgg tcgggtcgga
     2281 cgggcacgtg agcagcgtac cgcccatgcc gtcatccacc agcacggcga cgttggtcag
     2341 caggtcacag gcttcgtaga cgccgtcgct gttgtcgtcc acacagcaaa ggtcgcgtgt
     2401 cgtgcaggtg tagctgccgg actcgtcgta gcaataggcc gatgggtccg actgtacgta
     2461 acagatctct ccgctgtatt cgaatagacc ggtcaggtcg gtcgccttgc gcacgccctt
     2521 gcccttggtc gagccgctag gatcggtgcg gtagagaatg gtgccatccg aaccgaaggt
     2581 ggccgtgccg ctcgagtcga cgaggccgag tagtacgaga tcgttgccgg cctcgtcctc
     2641 gacgtagaaa agatccggcg aaatcgaaac agttggctcg ccatccttga gcggtttgcc
     2701 cccgagcgtg ccgtacaccg cgtaaccgtt cgcgttgtag ggtagccgca ggacggcgct
     2761 gtcaccgctc gcctggccga agtcgggaaa gctttccgtg caccagtcgg tgacctcgag
     2821 agtttcggta tccggtgctc ctttcggccc cttggtgccg gactccatca ggaccgtgat
     2881 ggggtcctct ccctgtacac gcgggaaaaa aatgaccttg ccaaactcct ggttgccgag
     2941 ctcgtcgaag ctcggttccg ggcaggtgaa gtgatccgcc ttggcggaga ggttcaggtt
     3001 gaaatgcgca ccgctgggaa atccgttgcc aaacggcaga cccgaggaat ccacgggctt
     3061 ggcggcatgc gcctcgacag aggcgagcaa tgcggttgat gcgaggacga cgatcagcgg
     3121 cttgcgggat aatcttccgt tcatgactta ccctccagat ttacagatgc gggccggcgg
//
LOCUS       DASUOM010000640         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1592774,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000640 DASUOM010000000
VERSION     DASUOM010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592774"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /gene="purE"
                     /locus_tag="VFY27_13430"
     CDS             complement(<1..396)
                     /gene="purE"
                     /locus_tag="VFY27_13430"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5766561.1"
                     /translation="MNSEPKNAAVGIIMGSRSDWETMRHAADVLEELGIACDIQVVSA
                     HRTPDLLFEYASTAIERSLKVIIAGAGGAAHLPGMTAAKTRLPVLGVPIRSKALQGMD
                     SLLSIAQMPAGIPVGSMAIGKAGAINAALL"
     gene            complement(393..908)
                     /gene="yjgA"
                     /locus_tag="VFY27_13435"
     CDS             complement(393..908)
                     /gene="yjgA"
                     /locus_tag="VFY27_13435"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX5766562.1"
                     /translation="MNDAKPSKSARKRDHLALQTLGEKLIPLELSELEQLPLDEPLLE
                     AIVYAKKIRSRSALRRQRQLIGKLMREVDAESIEASLQKLSRQDQLSRETFRKAEGWR
                     DRFLEDGLGAVKKFAAQTGHPEAEFELLLRELDASGSEPAKRAVRRRIFREVYKVLAK
                     AGANAKGVEGQ"
     gene            1098..2444
                     /gene="pmbA"
                     /locus_tag="VFY27_13440"
     CDS             1098..2444
                     /gene="pmbA"
                     /locus_tag="VFY27_13440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX5766563.1"
                     /translation="MSVQAHRVTLDQRALEDIVELTLQEARKLGADQAEAAASHDIGL
                     SVTARLGDVENLEYTNDRGVGITIYMGSRKGSASTSDFNPEALREAVEKACSFAKYTA
                     PDKYAGLADKELMANDPPELDLAHPWELDSETAIRLAIECEDAARSFDKRISNSEGAT
                     VATNAGVRAYANSHGFVGSYRKTSHSMSCVVVGETNGHMERDHWYSAARDPADLESPE
                     KIGTTAARRTVERLGARKIKTTKAPVVFAPEVARGFIGHAVGAISGGAQYRRSSFLLD
                     AAGKQIFPDFFRIEERPHIPKALASAAYDAEGVATRDRDVVVDGVLQGYILSSYSARR
                     LGLVTTGNAGGAHNLIVPGNAEDMHALLQMMGDGLLVCEMIGQGVNGVTGDYSRGAVG
                     FWVENGQVAFPVHEITIAGNLKDLYMRIRAVGRDQDLRGGIRCGSLLVDGLTIAGA"
     gene            complement(2375..3793)
                     /locus_tag="VFY27_13445"
     CDS             complement(2375..3793)
                     /locus_tag="VFY27_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5766564.1"
                     /translation="MIPELQEKIEKTVWGNALQKRGRLGQIASHLLRYLYALIRDILA
                     GQLTLRAMSLVYTTLLSVVPLIAMSFSVLKGLGVHNDLRPLLYEGLAPLGEQGEEITD
                     QVINLVDNVRGGLLGGVSLAFFIFTSVSMVQKVEESFNFVWHVGQPRSIARRFSEYLV
                     VMLVGPVVIVIAIGMIASIRNASFVQMILDNDALGPVFVGAGKVMPYLLVASVFTFLY
                     MFVPNTKVKLTSALVGGFVGGCIWAGMGVIFTTFVLYATQRELIYSGFAVAITTLIWL
                     YLSWLVLLIGAEVAFYHQRPAFLRVGRREPSLSNSMRECLALNVMFLVGKAFRDKDRS
                     LTLEGICAQLKVPSIALEPIVDRLEKEGLLIVTEKEELLPGREMARMQLVDILDVVRQ
                     RGDTGSYRGPAWSADIESIGEQLEKAVSDVAGSQTLSDLLDRAEKEKEGVPAAGPRTS
                     HAPAIVKPSTSSEPQRIPPRRS"
     gene            complement(3895..>4443)
                     /locus_tag="VFY27_13450"
     CDS             complement(3895..>4443)
                     /locus_tag="VFY27_13450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5766565.1"
                     /translation="RGYLIGSGWRDYRASSQLCGMELPKGLEQAAKLPQTLFTPATKA
                     ESGQHDENISFETVADLLGQRLAERVRNLAVTIYERAAAYARERDIIIADTKFEFGQD
                     DEGRLYLIDEVLTPDSSRFWPASEYRTGISPPSFDKQFVRDYLDTLDWDKTPPGPSLP
                     PDVIARTAEKYRRALVLMTGHG"
BASE COUNT          856 a         1387 c         1359 g          841 t
ORIGIN      
        1 gagcaacgcg gcgttgatcg caccagcctt gccgatcgcc atgctgccca ccggaatacc
       61 cgccggcatc tgcgcgatgg acaacagcga atccatgcct tgcagggcct tcgaacgtat
      121 cggcacgccg agcacgggca aacgcgtttt tgcagcagtc attcctggaa gatgggcggc
      181 gccaccggcg cccgcgatga tgaccttgag actccgctct atcgccgtcg aggcatactc
      241 gaacaggaga tcgggcgtgc ggtgcgccga gacgacctgt atgtcacagg caattccgag
      301 ttcctccaga acatccgcgg cgtgccgcat cgtttcccag tcggagcgag agcccatgat
      361 gatgcctacc gctgcgtttt tcggttcgct gttcattgcc cttcaactcc cttcgcgttc
      421 gcgcctgctt tcgccagcac tttgtatact tcccgaaaga tgcgccggcg aaccgcgcgc
      481 ttcgcgggct cgcttccgct cgcgtcgagt tcgcgaagca gcaattcgaa ttccgcctcc
      541 ggatggcctg tttgcgccgc aaatttcttt acagccccaa ggccgtcttc caaaaatcgg
      601 tcgcgccaac cctcggcttt gcgaaacgtc tcgcgcgaga gctggtcctg gcggctgagc
      661 ttttgcagcg acgcctctat cgattcggcg tccacctcgc gcatgagctt gccgatcagt
      721 tgccgctggc gcctgagcgc gctccgtgag cggattttct tcgcgtatac gatcgcctcc
      781 agcagcggct cgtccagcgg cagttgttca agctccgaga gctcgagtgg aatcagtttt
      841 tcgcccaggg tctgcaacgc aagatgatct ctcttgcgcg cgcttttgct gggctttgcg
      901 tcgttcaccc gctaagctac ctcgtgaccg gacatgcgag cagattgctt cgagtgcggc
      961 atagtatcat gccgacacag gcacttacgc cacgcagcgc ctgttcacgg ccgcccggta
     1021 tactcatccc gcaagcgcgg aacgaccatc agatacgaaa ccatcaagta caacaccgtc
     1081 aggtacgaaa ccctgctatg agcgtacagg cacatcgcgt gacgctggat cagcgcgcgc
     1141 tcgaagacat cgtggagctt acactgcaag aagcacgcaa actcggcgca gaccaggcgg
     1201 aggctgcggc aagtcacgat atcgggttaa gcgtgacggc gcggcttggc gacgttgaaa
     1261 acctcgagta cacgaacgac aggggtgtcg gcatcaccat ttacatgggg tcgagaaagg
     1321 gcagcgcgag tacttccgat ttcaatcccg aggcacttcg tgaagcagta gaaaaagcct
     1381 gttcatttgc gaaatatacg gcgccggaca agtacgccgg tctcgcggac aaggagctga
     1441 tggcgaatga tccgccggag cttgatctcg cgcacccgtg ggagctggac tccgagacgg
     1501 ccattcgcct tgcaatcgaa tgtgaggacg ccgcgcgcag cttcgacaag cgcatcagca
     1561 actcggaagg cgcgactgtc gccacgaatg cgggcgtcag ggcatatgca aattctcacg
     1621 ggttcgtcgg cagttaccgc aagacgagtc acagcatgag ttgcgtggtc gtgggagaga
     1681 cgaacggcca catggagcgg gatcactggt acagcgcagc ccgcgacccg gcagatctcg
     1741 agtcgccgga gaagataggc actacagccg cgcggcgcac ggtcgagcga ctgggggcac
     1801 gcaagatcaa aaccaccaag gctccggtcg tgttcgcgcc cgaggttgcc cggggcttta
     1861 tcggtcacgc ggtaggcgct atctcgggcg gcgcgcagta ccgccgctcg tcgttcctgc
     1921 tggacgcggc cggcaagcag atctttcccg attttttccg catcgaggaa cgcccccaca
     1981 ttccgaaagc gctggcgagc gcggcgtacg acgccgaagg cgtcgccaca cgggatcgcg
     2041 acgtcgtggt cgacggggtg ttgcagggat acatcctgag ctcctattca gctcgtcgcc
     2101 tcggcctggt caccaccggg aacgccggtg gcgcgcacaa cctgatcgtg cctggcaacg
     2161 cagaagacat gcacgcgctt ttgcagatga tgggggatgg actgctcgtg tgcgagatga
     2221 tcggccaggg cgtgaacggt gtgaccggcg actactcgcg cggtgccgta ggtttctggg
     2281 tcgaaaacgg ccaagtggcc ttcccggtgc atgagatcac gatcgccgga aacctgaagg
     2341 acctgtacat gcggatccgc gcagtcgggc gcgatcagga tctccgtggg ggtatccgct
     2401 gcggctcact gctggtagac ggtttgacga tcgccggcgc gtgactcgtg cgcggcccgg
     2461 ctgcaggcac gccttctttt tccttctccg cgcggtcgag caggtccgac agggtctggc
     2521 tccccgcgac atcgctgacc gccttctcga gctgttcgcc gatcgattcg atgtcggccg
     2581 accacgcagg accgcggtac gacccggtat cgccacgctg ccggacgaca tcgagtatgt
     2641 caacgagttg catgcgcgcc atctcgcgcc ccggcagcaa ctcctccttt tcggtcacga
     2701 tcagcaggcc ttccttctcc agcctgtcga caatcggttc cagggcgatt gacgggacct
     2761 tgagctgcgc gcagatgcct tccagtgtca ggctccggtc cttgtcgcga aatgccttgc
     2821 cgaccaggaa catcacattg agtgcaaggc actcgcgcat cgagttcgac aggctcggct
     2881 cgcggcgccc tacgcggagg aatgcgggac gctggtgata gaacgctact tcggcaccga
     2941 tcagcagcac cagccagctc aggtaaagcc agatcagtgt ggtgatggcg acggcgaaac
     3001 ccgaatagat aagctcgcgc tgcgtcgcat acagcacgaa ggtcgtgaag atcacgccca
     3061 tgccggccca gatgcaaccg cctacgaagc cgccgaccag cgcggacgtt aacttcactt
     3121 tcgtgttcgg cacgaacata tacaggaaag tgaatacgct cgcgaccagc aggtagggca
     3181 tcaccttccc cgctccgacg aagaccgggc caagcgcgtc gttgtccagg atcatctgga
     3241 cgaagctggc attgcggatc gaggcgatca tgccgatggc gatgacgatg acgacaggcc
     3301 ccaccagcat gacgaccagg tactcggaga agcgccgcgc gatgctgcgc ggttgcccga
     3361 catgccacac gaaattgaag ctctcttcga ccttctgcac catcgatacc gatgtgaaga
     3421 tgaagaacgc aaggctcacg ccgccgagca ggcctcctcg cacgttgtcg accaggttga
     3481 tgacctggtc agtgatttcc tcgccctgtt cgccaagcgg cgcgagtccc tcatacagga
     3541 gcggccgaag gtcgttgtgc accccgagtc ctttcaggac cgagaagctc attgctatca
     3601 gcggcacaac cgacaacaac gtcgtataca caaggctcat tgcacgcagc gtgagctgcc
     3661 ccgcgaggat gtcgcggata agcgcgtaaa ggtagcggag caggtgcgat gcgatctggc
     3721 ccagccgccc tctcttctgc agcgcgttgc cccagacggt tttttcgatc ttttcttgca
     3781 gttccgggat catggcgggg tgctgtaccg ggcaggttga ggctcaggga ccctgaattg
     3841 taacccaggc tttcgcgctt cccgcgggaa gcgggtcacg ctggaggcgg ccctttagcc
     3901 gtgcccggtc atcaggacca gcgcccggcg gtatttttcc gccgtacggg cgatcacgtc
     3961 cggcggcagg gaagggccgg gcggagtctt gtcccagtcc aaagtgtcca ggtaatcgcg
     4021 aacaaattgc ttgtcgaagc tgggagggct gattccggtc cggtactcgc tcgccggcca
     4081 gaaacgcgaa gagtcgggtg tgagcacctc atcgataaga tacaggcgac cttcgtcgtc
     4141 ctgcccgaac tcgaacttgg tgtccgcaat gatgatgtcg cgttcgcggg cgtaggccgc
     4201 tgctctctcg tagatcgtaa cggcgagatt gcgcacgcgc tccgcaagcc gctgcccgag
     4261 gaggtcggct acggtctcga acgagatgtt ctcgtcgtgc tgaccgcttt ctgccttcgt
     4321 tgccggcgtg aacagcgtct gcgggagctt tgcggcctgc tcgagcccct tcggcagctc
     4381 catcccgcaa agctggctgg atgcccggta gtcacgccag cccgagccga tcagatagcc
     4441 cct
//
LOCUS       DASUOM010000641         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1182557,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000641 DASUOM010000000
VERSION     DASUOM010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182557"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFY27_13455"
     CDS             complement(<1..195)
                     /locus_tag="VFY27_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766566.1"
                     /translation="MTPWDKLATKFDRKRYALCRRGGKGRYGGSNAKQSEMTERALPP
                     GLNGDIDVLAFAEAELAAGQP"
     gene            221..1006
                     /locus_tag="VFY27_13460"
     CDS             221..1006
                     /locus_tag="VFY27_13460"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5766567.1"
                     /translation="MGLAEGRLIVEGDGPIGWLTFNQPERLNAVRLDMWQALPEAVGL
                     LSGDPSVRAIVLRGAGDRAFISGADIAEFELERSSSPSNQVFVRTVSAATAALIETRK
                     PVIAMIRGFCIGGGMVIASACDIRICAEDSRFGVPAAKLGLGYELDNLALLQSIVGKG
                     IAMEMLATARQFSADEALRTGFVNRVVPAADLEECVRELAVQIARNAPQSIAAAKLAS
                     RAASNPSLTEEAQRAVDACFDSADYQEGRAAFRQKRAPRFTGK"
     gene            1015..2577
                     /locus_tag="VFY27_13465"
     CDS             1015..2577
                     /locus_tag="VFY27_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5766568.1"
                     /translation="MSMTTTYELLEQPFATMPEMIRTHAQERPAHRAIIDGERVVTFT
                     ELDTLIDRAAAAMQRDDVGNLDAVAICSPSSIEYVVAFVAALRVGAAACPLAPSSTPE
                     QLTAMVRDCGAGLLFVDDSVSTALDRATERLNVKRVSLEGAANPSFASWLAPAGARPV
                     QTEIDPEQPFNIIYSSGTTGTPKGIVQPHRMRWGHIRRAEVLGYGSDSITVVSTPLYS
                     NTTLVSLIPALVRGGTVVLMRKFDARGFLELSQKHRATHAMLVPVQYRRLMDLPDFDS
                     FDLSSYRMKTCTSAPFAADLKADIVKRWPGGLVEIYGMTEGGGSCLLRAHEFPNKLHT
                     VGQPAPGHDVRIIGEDGKELPRGETGEVVGRSMSIMSGYHNQPQKTAEAEWLGPQGER
                     FIRTGDVGRFDEDGFLVLGDRKKDMIITGGFNVYPSDLEAELARHPAVAESAVVGVPS
                     REWGETPAAFVVLRGDETVDPDALKQWLNARVGKTQRLAYLTIVPTLPRSSIGKVLKR
                     ELRESFKGQHTA"
BASE COUNT          514 a          851 c          884 g          508 t
ORIGIN      
        1 aggctggcca gccgcaagct cggcctctgc aaacgccagc acgtcaatgt cgccattcag
       61 ccctgggggg agcgcgcgtt cggtcatctc gctctgtttt gcgttcgagc cgccatagcg
      121 ccccttgcct cccctccggc atagggcata gcgcttcctg tcgaatttcg tagccagctt
      181 gtcccaaggg gtcaacgcag gccttcaggg atgcaccgcc atgggcctgg ccgaaggccg
      241 gttgatcgtt gagggggacg ggccaatcgg ctggctgacc ttcaaccagc cggagcgcct
      301 gaatgccgtt cgcctggata tgtggcaggc gctgccggaa gccgttggac tgctatctgg
      361 ggatccttcg gtgcgggcta tcgtcctgcg aggcgcaggc gaccgtgcct tcatttctgg
      421 cgcagacata gccgagttcg aactggagcg ctcgtcttcc ccttccaatc aggtgtttgt
      481 gcgtactgta agtgcggcaa cagcagcact tatcgagacc cgcaagccgg tgatcgcaat
      541 gatccgtggc ttctgcatcg gtggcggcat ggtgatcgcc agcgcctgcg atattcgcat
      601 ctgcgccgaa gactcacgct ttggcgtgcc ggcagcgaag ctcgggctcg gctatgaact
      661 cgacaacctg gcgctcctgc agtccatcgt aggcaaaggc attgcgatgg agatgctggc
      721 gacggcgcgc cagttttccg ccgacgaggc ccttcggact gggttcgtca accgagtggt
      781 tccggcagct gatctcgaag aatgtgtgag ggagttagcg gtgcagattg cacggaatgc
      841 gccgcagagc atcgccgcgg cgaagctggc aagccgcgca gcttcgaatc cgtctttgac
      901 agaagaggcg cagcgggccg tcgatgcctg ctttgacagc gcagactacc aggaaggccg
      961 cgccgcgttc cgccaaaagc gcgcgccgcg cttcactggc aaatagtacc agggatgagc
     1021 atgacaacta cctatgaact tcttgaacag ccgttcgcta caatgcccga aatgatccgc
     1081 actcacgcgc aggaacgacc ggcacatcgc gcaatcatcg atggggagcg tgtcgtaaca
     1141 ttcacagaac tcgacaccct gatcgatcgc gccgcagcag cgatgcagcg cgatgacgtc
     1201 ggtaacctcg acgcggttgc aatctgcagt ccgtcgtcga tcgagtatgt cgtagccttc
     1261 gttgcagccc tgagagttgg cgctgccgcg tgcccgctgg ccccgtcttc gacgcccgag
     1321 cagctcacgg cgatggtgcg cgactgcggc gccggcctgt tgttcgtcga cgacagcgtg
     1381 tccacagccc tggacagggc gaccgaaaga ctgaacgtaa agcgcgtctc acttgaaggc
     1441 gccgcgaatc cctcgtttgc gtcgtggctg gcgcctgcag gagcccgtcc ggtgcaaacg
     1501 gagatcgatc ccgagcagcc attcaacatt atctattctt ccggcacgac gggaacgccc
     1561 aaaggcatcg tccagccgca caggatgcgc tggggccata tccggcgtgc cgaagtgctg
     1621 ggctacggat cagactccat cactgtggtc tcgacaccgc tttattccaa caccactctt
     1681 gtgagtctga tcccggcgct cgtgcgcggc ggaacggtcg tgctgatgcg aaaattcgat
     1741 gcgcgcggct ttctcgagct ctcacagaag catcgcgcca cgcacgccat gctggtgccg
     1801 gtgcagtatc gacgcctgat ggacctgccg gatttcgaca gcttcgacct ttcatcctac
     1861 cgtatgaaga catgcaccag cgcgcctttc gccgccgatc tgaaagccga catcgtgaag
     1921 cgctggccag gcggcctggt ggagatctac ggcatgacgg agggcggcgg atcctgcctc
     1981 ctgcgtgcgc acgaatttcc gaacaagctg cacacggtcg gccagccggc gccgggacac
     2041 gacgttcgca tcatcggcga ggacggcaag gaattaccgc gaggtgagac tggagaggtt
     2101 gtcggccggt ccatgtcgat catgagcggc taccacaatc agccgcagaa aacggcggaa
     2161 gctgaatggc ttggccctca aggtgagcgg ttcatccgga caggggacgt tggccggttc
     2221 gatgaggacg gcttcctggt gctgggcgat cgcaagaagg acatgatcat cactggcggc
     2281 ttcaacgtct atcccagcga cctggaggcc gagctggcca ggcatcctgc ggtagccgaa
     2341 tccgcggttg tcggggtccc gtctcgcgaa tggggcgaaa cgcccgccgc gtttgttgtg
     2401 ctgcgcggtg acgagactgt cgaccccgat gctctcaagc agtggctgaa tgcccgcgtg
     2461 ggaaagacgc agcgactggc ttacctcaca atcgtgccga ccctgccccg ttcgtccata
     2521 ggcaaggtgc tgaagcgcga gttgcgcgag agcttcaagg gacagcatac cgcctgaccc
     2581 ccggctcgat gacgtcgccg gattgcccgc tcggggcggc ccgctctatg gtcggcgaaa
     2641 accgggcgag gacatccatg agcgaggcgc gggccgcagg ccgcgaggac gatcaaatgg
     2701 tcgctcaggg catgatggtt gcgctgcatg cgcgccgcat gccggaggac gcggcgg
//
LOCUS       DASUOM010000642         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1667509,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000642 DASUOM010000000
VERSION     DASUOM010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667509"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFY27_13470"
     CDS             complement(<1..1010)
                     /locus_tag="VFY27_13470"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5766569.1"
                     /translation="MAARTLKRVFYGALGATGVALALFAFYLLSRTAQDAGEFDQLQN
                     LILLVNVAGVLALLALLVGNLARLVRDYRAHVPGAKLKARMVAMFVGLAVLPLLVVFY
                     FAMQFINRGIDTWFNVEVEAGLDDALALSQAALGTRMRDHLNTTIQIAERLRDVDGRE
                     LIFQLGLLRRESGASEITLFGRGGRIIAMSSDELADSLPEPAKEEMLMQIHQDRPYVN
                     LDSVEEGIYEIRTAVRFSTDGREGQAFVRAVFPVNERLGRMASNVERSYSDYKRAVYL
                     REPLKRTISITLTVVLMISLLASIYGAFFFSRRLVAPIQDLVAGTRAVAKGDFDTRLP
                     TPS"
     gene            complement(992..1585)
                     /locus_tag="VFY27_13475"
     CDS             complement(992..1585)
                     /locus_tag="VFY27_13475"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX5766570.1"
                     /translation="MSGLRRANISALVLGLSLALPFVVMVHAQDRPGREGYFDVRSAS
                     TAVVEDVIELEARLQLVLSDEALAALDSGVPLTIELKLEVIRERRFLPDATDAELTLR
                     YELEYRPLSQRYIVRNLNSGNQDSFATLYSALNNLGRIQDLPVVDESILDPDADYRVR
                     LQALLNTQQYPAPLRLLFFWRDQWQLKSEWFEWPLER"
     gene            complement(1582..2976)
                     /gene="rsmB"
                     /locus_tag="VFY27_13480"
     CDS             complement(1582..2976)
                     /gene="rsmB"
                     /locus_tag="VFY27_13480"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX5766571.1"
                     /translation="MSAPNTGAAVRAEAARAIDAVATAGRSLDEVLASAEAATDPADR
                     PLLRMLCYGTLRNHWRLREFIGQLLDKPLKAADSVIESLIALGLFQLVESRIPDHAAV
                     SLTVDAARRLRKPKLAPLVNAVLRNFVRRNLAATGPSTLESRFNHPAWFIERLQQDWP
                     EHWQEILLANDRRAPMWLRVNRRKTSAREYLKLLDGGKLQEGPGEAGPASPAMLEGLE
                     QAIRLAAPRAVTDLPGFADGVVSVQDAAAQLAAPWLLGHVPPPDAALRILDACAAPGG
                     KTAHVLELCTPSATLTAVDADSRRLAFVGDNLERLGLAATLVAADASNPEAWWDGEPF
                     DRILLDAPCSGTGVIRRHPDIKLLRRKTDIDKSAELQLALLEALWRLLAPGGRLLYAT
                     CSVLAAENDEVVSRFLSRTPEAIEDNGEQVLPNYNIRDLMHRKRRGFQILPGERDMDG
                     FYYACLDKPGRMTG"
     gene            complement(2973..3917)
                     /gene="fmt"
                     /locus_tag="VFY27_13485"
     CDS             complement(2973..3917)
                     /gene="fmt"
                     /locus_tag="VFY27_13485"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5766572.1"
                     /translation="MPHNLVFAGTPRFALASLRALVQNDMAPVLVLTQPDRPAGRGRK
                     LKASPVKLYAKEQGLTLSQPETLKDPLLMDHLAELEPTAIVVAAYGLIFPPELLALPR
                     QGCVNVHASLLPRWRGASPVQSAILGGDSVTGISLMRMEAGLDTGPVYLQQSIDIGTA
                     ETAGGLGERLGELGGELLAQHLAQILDGSLRPEAQDESRATYARKVDKAQARLDFALP
                     AVQLERQVRAYDPEPGAWFMLRDERVKCWKAEIAPGNSRHAAPPGTVLQVSRAGIEVA
                     CGEGAIRLLELQRPGKDRVTGGSQFGDGMVTPGTSLPV"
     gene            complement(3945..4469)
                     /gene="def"
                     /locus_tag="VFY27_13490"
     CDS             complement(3945..4469)
                     /gene="def"
                     /locus_tag="VFY27_13490"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5766573.1"
                     /translation="MAILPILEFPDPRLRKKAALVAHVDEELNRLIDDMFETMYAAPG
                     IGLAATQVDVHQCLLVADVSTERTAPYVLINPEILEKEGVAITEEGCLSVPGFYEEVE
                     RANHIKVRFLDRKGAVNEQEFEGLMAVCVQHEIDHLNGRLFVDYLSEAKRQRIRKRLE
                     KERRQQAVPEAVGS"
     gene            4920..5912
                     /locus_tag="VFY27_13495"
     CDS             4920..5912
                     /locus_tag="VFY27_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5766574.1"
                     /translation="MEQTERRVPLAEGHPDEYVVQPGDTLWDIASTFLRDPWYWPEIW
                     HVNPEIGNPHLIYPGDVLALVTIDGETRIMAVRGSSYRLSPQARVTPLDESIRSIPYE
                     EIAAFLSKGLVLEKNQIEQLPYVLAVRENHLIAAAGNDVYVRGGEPAQPGTRYSLVHV
                     GDELIDPDDNQVVGYQGIYVGEGALSRGGDPATVALTETNREALRGDRLLPESVDIPL
                     NFFPKSPDSEIDGRIISVVDGLYLIGQYQTVVLNRGARDGLAPGDVLTVFQEGEVVKD
                     RFAGASMFSGEKVKLPDEEAGTVMVFKVYDRIGYGLVMQATSDIHVLDAVRNPR"
     gene            6015..>6668
                     /locus_tag="VFY27_13500"
     CDS             6015..>6668
                     /locus_tag="VFY27_13500"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5766575.1"
                     /translation="MTGPERAWFILAKARLNQGCLKQLTDEFETAERIVSLPARELRK
                     LGLGEAALDAICNPDEAAIANDLRWLSESGNHLVHPQSEFWPELFRDLVDAPTILYLR
                     GRPESLHLPCLAIVGSRNPTRGGVLNAFDFARHMGAAGFAIVSGLAQGIDTAAHKGAL
                     DADATTIAVLGHGIDRVYPAANRELAHRIAAQGALVSEYPLETHPRKEHFPERNRLIS
                     "
BASE COUNT         1243 a         2156 c         2030 g         1239 t
ORIGIN      
        1 gaaggcgtcg gcagtcgcgt gtcgaagtcg cccttcgcga ccgcccgcgt accggcaacg
       61 agatcctgga tgggcgctac gagacgccgc gaaaagaaaa aggcgccgta gatggacgcg
      121 agcagggaga tcatcaggac caccgtcaag gtgatcgaaa tggttcgctt caacggttcc
      181 cggaggtata ccgcgcgctt gtagtcgcta taggatcgtt cgacgttcga tgccatgcgg
      241 ccaagccgct cattcaccgg aaacactgcg cgaacgaatg cctgcccctc ccggccgtcg
      301 gttgagaagc gcactgcagt gcggatttca tagatgcctt cttcgactga atccaggttc
      361 acgtaaggac ggtcctggtg aatctgcatc agcatttctt ctttcgcagg ttccggaagg
      421 ctgtcggcga gttcgtccga actcatagcg atgatccgcc cgcctcgccc gaacagcgtg
      481 atctcgctgg caccactttc ccggcgcagc agtcccagct ggaaaatgag ttcccgccca
      541 tcgacgtcgc ggagccgttc ggcgatctgt atcgtcgtgt tgagatgatc acgcatccgc
      601 gttccgagcg cggcctggct cagagcaagc gcatcgtcga ggcctgcttc gacttccacg
      661 ttgaaccagg tgtcgatgcc gcggttgatg aactgcatcg caaagtagaa cacgaccagc
      721 agcggcagca ccgcgagccc gacgaacatt gcgaccatgc gggctttgag cttcgcaccc
      781 ggcacatgcg cacgatagtc ccgcaccagc cgcgcaaggt ttccaacgag gagtgccagc
      841 agcgcaagta cgccggcgac gttcaccagc aggatcaggt tctgcagctg gtcgaactct
      901 ccggcgtcct gcgcagtacg actcagaaga taaaaggcga acagcgccag ggcgacgccg
      961 gttgcgccga gcgcaccgta aaaaacccgc ttcagcgttc gagcggccat tcgaaccact
     1021 cactcttgag ttgccactgg tcgcgccaga aaaacaatag ccgcaacgga gcaggatact
     1081 gctgcgtgtt caaaagcgcc tgcagccgaa ctcggtaatc ggcatcagga tccaggatcg
     1141 attcgtccac caccggcaag tcctggatgc gtcccaggtt gttcagcgcc gagtacaggg
     1201 ttgcaaacga gtcctggtta ccgctgttca ggtttctcac gatgtagcgc tggctcaggg
     1261 gccggtactc gagctcgtaa cgaagggtca gttccgcatc ggtggcgtcc ggcagaaagc
     1321 gccgctcccg tatcacttcg agcttgagtt cgatcgtcag cggaacgccg ctgtcgagtg
     1381 cagccagcgc ttcatcactc agcacgagct gcaggcgcgc ctcgagctcg atcacgtcct
     1441 cgacgacggc ggtcgacgcc gaacgaacgt cgaaatagcc ttcgcggccg ggacggtcct
     1501 gggcgtgaac catcacgacg aagggcaacg caagcgagag cccgagtacg agcgccgaga
     1561 tatttgccct gcgaagtccg ctcatcctgt catccggccg ggtttgtcca ggcacgcgta
     1621 gtaaaagccg tccatatccc tctcgccggg caggatctgg aagcctcgtc tcttgcgatg
     1681 cattaaatcg cggatgttgt aatttggcaa cacctgttcc ccgttatctt cgatcgcctc
     1741 cggcgtccgg ctcaggaagc ggctgacaac ctcgtcgttt tcggcggcca gcacggagca
     1801 ggtggcatag agaagccgtc ctccgggcgc aagcagtcgc cagagggcct cgagcagggc
     1861 cagctgcaat tccgcgctct tgtcgatgtc ggtttttcgt cgcagcagct ttatatccgg
     1921 atgacggcgg atgacgccgg tgccgctgca aggcgcgtcc agcagtattc ggtcgaatgg
     1981 ctcgccgtcc caccacgctt ccggattaga tgcgtcggcg gcaacaagtg tcgcagcgag
     2041 ccccagccgc tcgagattgt ccccgacgaa ggccaatcgc cggctgtccg cgtccaccgc
     2101 cgtcagagtg gcggaaggcg tgcagagttc cagcacatga gcggtcttgc cgcccggggc
     2161 cgcgcaggcg tcgagtatgc gcaacgccgc gtccggcggc ggcacgtgcc caaggagcca
     2221 tggagccgcc agctgcgcgg ctgcatcctg tacggagacg accccgtcag cgaacccggg
     2281 aaggtcggtc accgcgcgcg gtgcggcaag ccggatcgcc tgttcaagac cttcgagcat
     2341 cgcagggctt gccggacccg cctcgccggg accttcctgg agcttgccgc catcgagcag
     2401 cttcaggtat tcgcgagcgc tggttttccg ccggttcacc cgcagccaca tcggcgccct
     2461 tcggtcattg gcgaggagga tctcctgcca atgctccggc cagtcctgtt gcagccgctc
     2521 gatgaaccac gccgggtggt tgaaccgcga ctcgagcgtg gacggccccg tcgccgcgag
     2581 attcctgcgc acgaagttcc tgagcacggc gttcacgagg ggtgcgagtt ttggcttgcg
     2641 caggcgccgg gcagcatcga ccgtcaggga tactgcggcg tggtccggaa tgcggctttc
     2701 aaccagctgg aacagaccga gggcgatcaa agactcgatg acgctgtccg cggccttcaa
     2761 cggcttgtcg agcaactgac cgataaactc gcggagccgc cagtgattgc gcagcgtgcc
     2821 gtagcaaagc atccggagaa gaggccggtc agccggatcc gtcgcggcct ctgcgctcgc
     2881 caggacctcg tcgagtgagc gaccggcggt tgcaacggcg tcgatcgcgc gggcggcctc
     2941 ggcgcggacc gcggcaccgg tattgggcgc gctcataccg gcaacgaagt gccgggggtc
     3001 accatcccgt cgccaaactg cgagcccccc gtaacgcggt ctttgcccgg cctctggagt
     3061 tccagcaggc gaatcgcacc ctcgccgcaa gcgacctcga ttcccgcgcg gctcacctgg
     3121 agcacggtgc cgggcggcgc cgcgtgccgc gagttgcccg gggcaatttc cgctttccag
     3181 cacttcactc gttcatcgcg cagcatgaac caggcgccgg gttcgggatc gtaggcccgc
     3241 acctggcgct cgagctgtac tgcaggcagg gcgaagtcga gcctggcctg cgccttgtcg
     3301 actttcctgg cataggtcgc gcggctttcg tcctgcgcct ctggcctcag gctgccgtcg
     3361 aggatctgcg caaggtgttg cgcgagcagt tcgccgccga gttcgccgag ccgctcccca
     3421 agcccgcctg ccgtctcggc ggtaccaata tcgatcgact gctgcaggta aaccgggccg
     3481 gtgtcgagcc ctgcttccat gcgcatcagg ctgatgccgg tcacgctgtc cccgcccagg
     3541 atagcggact ggacagggga ggcgccgcgc cacctcggca acagtgatgc gtgaacgttg
     3601 acgcagcctt gcctcggcag cgccaaaagt tcaggaggaa aaatcagccc gtaggccgca
     3661 acgacgatcg ccgtcggctc aagctccgcg aggtgatcca tcagcagtgg atctttcagt
     3721 gtttcaggct ggctcagcgt gagaccctgc tctttcgcgt aaagctttac cgggctcgcc
     3781 ttgagcttcc gccctcgtcc agccggccgg tccggctgcg tcagcacaag taccggcgcc
     3841 atgtcgttct gcacgagcgc gcgtagcgac gccagcgcga accggggcgt gccggcgaaa
     3901 accaggttgt gaggcatcgt cccggcagcc ggagctttgc tgcgtcagct accgacggcc
     3961 tcgggaaccg cctgctgtct tcgctccttc tccaggcgct tgcggatacg ctgtctcttg
     4021 gcctcggaca ggtagtcaac gaacaacctg ccgttcaggt ggtcgatctc gtgctgcacg
     4081 caaaccgcca tgagtccttc gaattcctgc tcgttgacgg cgcccttcct gtcgagaaaa
     4141 cgcaccttta tatggttcgc ccgttcaacc tcttcataga agcccggcac cgagaggcag
     4201 ccttcttccg tgatggcgac accttccttc tccaggattt ccgggttgat gagtacgtaa
     4261 ggagcagttc tctccgtgga gacgtccgcc accagcaggc actggtgaac atcgacctgc
     4321 gtggccgcga gcccgatgcc gggggccgcg tacatggtct cgaacatgtc gtcgatgagg
     4381 cggttgagtt cctcgtccac atgcgccacc aacgcagcct tttttctcag ccgaggatcg
     4441 gggaactcca gaatgggcag tattgccatg atactcgtgt ttaaactcgt gtgaattgct
     4501 ataaaatcga gctaaatttt tgactttatt gaataagata gcggacaatg ccgcctgaaa
     4561 atgcgcgggc caggctcgtg ccctgaacgg aattgtgacg tgcttggcag ccttccgcgg
     4621 ccttgaccta catattataa caagccggcc tggtcaggca ggccagagat cctttgacgg
     4681 agcaaccgcc aattatgttc ccagtaaaaa actgcttaac tttcagtcgc cgactgaccg
     4741 ttatcgcgct ggccatgccg ttggctgcat gctccatgaa tcccttcgcg aacgatgaca
     4801 gctcgtcccg ttcggcgccc accgccacaa gagccgccga cgccgtgccc gcttcttcgt
     4861 ccattgaacc gcgcacctcg gcccgccctg ccgtcgaacc gacttaccgg cctcagctcg
     4921 tggagcagac cgagcgccgg gtccccctgg cggaaggtca tcccgacgag tatgtcgtcc
     4981 agccggggga cacgctctgg gacatcgctt ccaccttcct tcgtgacccg tggtattggc
     5041 ccgaaatctg gcatgtcaac cccgagatcg ggaatcccca cctgatctac cccggcgacg
     5101 tactggcgct ggtgacgatc gacggtgaaa cccgcatcat ggccgtgcga ggctcttcct
     5161 accggctctc gccacaggcg cgggtgacac cactcgacga gtcgattcgc agcattccct
     5221 acgaggaaat cgcagcgttc ctttcgaagg gcctcgtact cgagaagaac cagatcgagc
     5281 agcttcccta cgtgctggcg gtgcgtgaga atcaccttat tgccgcggcc ggcaacgatg
     5341 tctatgtgcg aggcggtgag cctgctcagc ccggcacccg ctacagcctg gtccacgtag
     5401 gcgacgagct tatcgatccc gacgacaacc aggtcgtcgg ctaccaggga atctacgtcg
     5461 gcgaaggcgc actgtcccgc ggtggcgatc ccgcgacggt cgcactgacc gaaacgaacc
     5521 gggaagcgtt acgcggcgac cgcctgctgc cagagtcggt tgacatcccg ctcaatttct
     5581 tcccgaagtc gccggatagc gagattgacg gacgcatcat ctcggtagta gatggtttat
     5641 acctgatcgg ccagtaccag accgtcgtgc tgaatcgcgg cgcgcgcgac ggactcgccc
     5701 cgggagatgt tctgaccgtg ttccaggagg gcgaggttgt gaaggatcgc ttcgccggcg
     5761 cctccatgtt cagcggcgag aaagtaaagc tgccggatga agaagccggt acagtcatgg
     5821 tatttaaagt atatgaccgc attggctacg gacttgtcat gcaggcaacg agcgacatcc
     5881 acgtactcga cgccgtacgc aacccgcgct gacccgatcg aaggagggcc ggcatccgag
     5941 tggatgccgg ccctttttat ttgtcccgca gcctgtcccg ttcattctcc ctgtccagct
     6001 gaaggccgca acgggtgacc ggtccggaga gggcgtggtt catccttgcg aaagcgcgcc
     6061 tgaaccaggg ctgcctgaag caactcacgg acgagttcga gactgcggaa cgcatcgtct
     6121 ccttgccggc ccgcgagcta cgcaagctcg ggctcggtga ggcggcgctt gacgccattt
     6181 gcaatccgga tgaggctgcg atcgctaacg acctcaggtg gctctcggag agcggcaacc
     6241 atctcgtgca cccgcagtcc gaattctggc ctgagctgtt ccgcgatctc gtcgacgccc
     6301 cgaccattct ttaccttcgc ggcaggccgg agtcgctgca cctgccctgc ctcgcaatcg
     6361 tcggcagccg caatcccacg cgcggcggcg tgctgaacgc tttcgacttc gcccggcaca
     6421 tgggtgcggc aggcttcgca atcgtcagcg gacttgcgca gggaatagac actgccgcgc
     6481 acaagggcgc gctcgatgcc gacgccacca ccatcgcggt gctgggtcat ggaatcgacc
     6541 gggtgtatcc cgctgccaac agggaactcg cgcaccggat cgccgcacag ggcgcgttgg
     6601 tgtcggagta tccgctcgag acccatccgc gaaaggaaca ttttcccgaa cgcaacaggc
     6661 tcatcagc
//
LOCUS       DASUOM010000643         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_819820,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000643 DASUOM010000000
VERSION     DASUOM010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819820"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1486
                     /locus_tag="VFY27_13505"
     CDS             323..1486
                     /locus_tag="VFY27_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5766576.1"
                     /translation="MNLELNEDQRLIQNAAREFAQNEIAPIAARFDISGEFPAETIRK
                     AGELGFMGVEIPEEYGGAGFDAISYALVISEISAADAAHGTIISVNNSLYGTSLLDFG
                     TEEQKRELLEPVASGRVNAAYALTEPQSGSDAAAMRSRATVSPDGSLYTINARKSWIT
                     SGPVAKYIVLFARAEHENESPGISAFVIDTERPGFERGKTEPKLGIRASATCEIELTD
                     YKCPAEHRLGAEGEGFRIAMAALDAGRIGIAAQSLGIAEAAYAAALQYSRERKAFGSS
                     IGSFQAIQSKIADMKMRIEASRLLIYKAAWNKMEAKKAGAKNTLYGSLAKLFASETAM
                     YVAHQALQIHGGMGYSKELPLERYFRDAKITEIYEGTSEIQRMVIGRLETGIR"
     gene            complement(1643..1963)
                     /locus_tag="VFY27_13510"
     CDS             complement(1643..1963)
                     /locus_tag="VFY27_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5766577.1"
                     /translation="MPKVIYITPSGERHEVEVDNGYSVMEGAINNNIDGIVAECGGAC
                     ACATCHSYIDEAWLDKLPGMDDMEDSMLEAAYERKPNSRLTCQIEMNDELDGLIVHVA
                     ENEG"
     gene            complement(2173..>2621)
                     /locus_tag="VFY27_13515"
     CDS             complement(2173..>2621)
                     /locus_tag="VFY27_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766578.1"
                     /translation="KNYRLLSQAWSLAMEDEKAIPALQQAARLDRDGELYLRLANTHL
                     NLGEYDECVEAAREGLNVGGLKSPDHAQISLGMCLYNLKRYDDAIAAFRAASRNERSR
                     KMANQWIEVIESDIDRDEQIRLAEVAARRQQQALEQRRGGIDIGLR"
BASE COUNT          540 a          771 c          789 g          521 t
ORIGIN      
        1 aatctcctgc attcgaaacc gggtaatttg cgtgacacgc atgccattcg ttgccgacac
       61 tgtcacgcac cgcatcttaa gctgattgca gagcgtagtc atgagttccg gttgtatcca
      121 cacccacagc gcgtcccgac cggtacggag cctcccacgg agctagaata cttctaacat
      181 aatttcgtat ctcattaagt tttctatcta atttatccac aagaaacgca ccggcccaac
      241 gaatctcagg aagagttgac catagccgct ccgtgccagg cttcaccacc aacgtataat
      301 gtcccgctct acgggagaga agatgaatct tgaactgaac gaagatcagc gattgatcca
      361 gaacgcggcg cgggagtttg cgcagaacga gatcgcaccg attgccgcac gcttcgatat
      421 cagcggggag tttcccgctg aaacgatcag gaaggccggc gaacttggct ttatgggggt
      481 agaaattccc gaggagtatg gcggtgcggg cttcgatgcg atcagctacg cattggtcat
      541 ttccgaaatc tcggcagccg atgccgcgca cggcacgatc atctcggtca acaattcgct
      601 ttacggaacg tcgctgctcg atttcgggac agaagaacag aaacgcgagt tgctggagcc
      661 tgtggcatcg ggacgtgtga acgcggccta tgcactgacg gaaccacagt ccgggtccga
      721 cgcggccgcg atgcgttctc gcgccacggt ctccccggac ggctcgttgt acacgatcaa
      781 cgccaggaag tcctggatca cgtcagggcc ggtggcgaag tacatcgtac tgttcgccag
      841 ggccgaacac gaaaacgagt ctcccggcat cagtgctttc gtgatcgata cggagcgccc
      901 gggcttcgag cgcggcaaga ccgagccgaa gctcggcatc cgcgcttccg caacctgcga
      961 gatcgagctg acagattaca agtgccccgc ggagcaccgg ctgggagcgg aaggtgaggg
     1021 attccgcatc gcaatggcag cactggacgc gggcagaatc ggcatcgcgg cgcagtcgct
     1081 ggggatagcg gaagccgcct acgcggcagc gcttcagtac tcccgcgagc ggaaggcatt
     1141 cggcagctcg atcggcagct ttcaggcaat acaatcgaag atcgcggata tgaagatgcg
     1201 catcgaggca tcgcggctcc tcatctacaa ggccgcatgg aacaagatgg aagcgaagaa
     1261 agccggcgcg aagaacaccc tgtacggcag cctcgcgaag cttttcgcct cggaaacggc
     1321 catgtacgta gctcaccagg cgctgcagat ccacgggggg atgggttaca gcaaggaact
     1381 tccgctggaa cgctattttc gcgacgcgaa gatcaccgag atctacgagg gcaccagcga
     1441 gatacagcgc atggtcatcg gcaggctgga gaccgggata cgttagggac cgagtcgctc
     1501 ccctggcaat gcatatcgca ctcgcccgca tggcgggctc ctagaggtgc ctgtcggaac
     1561 agttttcaca tctcggcggg cgcgcaggga gtagcaacgc cgccaactcg ggacacgctc
     1621 gcatggcgag ctcctacggc ggttagccct cgttttccgc gacgtggacg atcaagccgt
     1681 ccagttcgtc gttcatctcg atctggcagg tcagtcggct gttcggtttc ctctcgtacg
     1741 ccgcttccag catgctgtct tccatgtcgt ccatcccggg caacttgtcg agccatgcct
     1801 cgtcaatgta gctgtggcag gtggcgcagg cgcacgcgcc tccgcactcg gcgacaatcc
     1861 cgtcgatgtt gttgttgatg gctccttcca ttacggaata gccattgtcg acctcgactt
     1921 catggcgttc gccgctgggc gttatgtaga tcactttggg catcggattg gtggtgtgcg
     1981 acacacgtcg ggctaaaagg cgcgccattg taaggacgcg gaccgagcag ggtcaacgcc
     2041 ggggatccgt cggaagatca tgtggaacgg ggtatataag taccggcgca ggaccatgca
     2101 ggtcaagcgc cggaagatga tgacccggta cggcggccgg cgaacaagta cagacgccgt
     2161 tattcctcgt ctctagcgaa ggccgatatc gatgccgccc cggcgttgct ccagggcctg
     2221 ctgttggcgg cgtgcggcga cttcggcaag gcggatctgt tcatcccggt cgatgtcgct
     2281 ctcgatgacc tcgatccatt gattggccat cttacgggaa cgctcgtttc tggacgccgc
     2341 gcggaatgcc gcgatggcgt catcgtaacg cttgaggtta tacaggcaca tgccgagcga
     2401 tatctgggca tggtccggac tcttaagtcc gcccacgttc agtccttccc ttgccgcttc
     2461 cacgcactcg tcgtactcgc ccaggttgag gtgcgtattg gccaggcgca ggtacaactc
     2521 gccgtccctg tccagccggg cggcctgttg cagcgcagga attgctttct cgtcttccat
     2581 cgccagggac catgcctgcg agagcagccg gtaattcttg g
//
LOCUS       DASUOM010000644         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_940710,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000644 DASUOM010000000
VERSION     DASUOM010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940710"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFY27_13520"
     CDS             complement(<1..228)
                     /locus_tag="VFY27_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX5766579.1"
                     /translation="MARIVEMHPLHPQPHRIASVVAAIREGGLIAYPTDSSYALGCHV
                     GDKKAMDRIHRIRRTDKNHNFTLVVRDLSEIS"
     gene            336..893
                     /locus_tag="VFY27_13525"
     CDS             336..893
                     /locus_tag="VFY27_13525"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HEX5766580.1"
                     /translation="MQLFVDYIPIVIFIVAYFYRDIFFATGVLMIAMPIILLIQWAKT
                     RKLNKIYLASTILVLVLGSATLFYRNATFLYWKPTVLNWAIATVFLGSQWIGNKPIVQ
                     RMLGSAADLSPAQWIRLNHMWIGFFLVVGAVNLFVAYNFSEEFWVKFKLFGMLGLTVL
                     FVIVQTVWLTAATKRNAITDDEGTD"
     gene            893..1192
                     /locus_tag="VFY27_13530"
     CDS             893..1192
                     /locus_tag="VFY27_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5766581.1"
                     /translation="MWYAVISEDIEDSLSRRSQARPAHLARLSTLVDEGRLLVAGPHP
                     AIDAENPGPAGFSGSLVIADFASLEEAEAWARDDPYVEAGVYANVVVKPFKWVLP"
     gene            1189..1461
                     /locus_tag="VFY27_13535"
     CDS             1189..1461
                     /locus_tag="VFY27_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5766582.1"
                     /translation="MNSLREARIRQCLRESLDPQELLIKDQSQLHIGHEGAKDGKGHY
                     DLLLVSRAFEGVNRVQRHRMVYDALRDLLESDIHALRISAYAPSER"
     gene            1508..2311
                     /locus_tag="VFY27_13540"
     CDS             1508..2311
                     /locus_tag="VFY27_13540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5766583.1"
                     /translation="MKSSNALTRVRIASVATVGALSLATATLVNAEAVKTVNGTEIDS
                     AVLDVYMESRVNKPIEQVTAEEREALIRELTDIYLLSTQDSATHLEDDPQVAAQIELQ
                     KRGVLAQAVASQFFAENKVTEEEVLAEYSEQVKLAPPLQYKARHILVPTQGAAKDVIS
                     ELDAGADFQEIAKEKSTGPSAPSGGDLGWFAPNQMVAEFSDSVAGLEDGKYTKEPVQT
                     EFGWHVILREESRQSEPPPLDSVRDALTQNVQQKKFQAHLDDLRANAEK"
     gene            complement(2567..>2884)
                     /locus_tag="VFY27_13545"
     CDS             complement(2567..>2884)
                     /locus_tag="VFY27_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766584.1"
                     /translation="GQTVLGSDSNWHGIALGTPVAVYGSIDRETGSIVNASVVPVAGG
                     DLSYLRGVVDEVNYSTGRAVVSGVTVDYNALLSTGHAPSIGDEVAVTGRVYQQLGLLV
                     AQP"
BASE COUNT          630 a          807 c          799 g          648 t
ORIGIN      
        1 gctgatttcc gacaggtccc gtaccaccag cgtaaagttg tgattcttgt cggtccgccg
       61 tatacggtgg atccggtcca tggccttctt gtcaccaacg tgacagccca gcgcatagct
      121 cgaatcggtg ggataagcga tgaggccgcc ttcccggatc gcggcaacca cgctcgctat
      181 ccggtgcggc tggggatgta acggatgcat ttcgacgatg cgggccatag gtggattgta
      241 acgggggtcc gggtgcccgg ttgcaagcaa ttttggcgcg gggcgagttt aggctattat
      301 tcgcgccctt tggcgaggct cgttacagga tcgaaatgca actattcgtt gactatattc
      361 cgatagtcat attcatcgta gcttatttct acagggacat tttcttcgcc accggagtac
      421 tgatgatcgc gatgccgatc atcctgctca tccagtgggc gaaaacgcgc aagctcaaca
      481 agatctacct tgcatcgacg attctcgtcc tggtgcttgg tagcgcaaca cttttctacc
      541 gcaacgccac gttcctgtac tggaagccga ctgtcctgaa ctgggccatc gcgaccgttt
      601 tcctgggtag ccagtggatc ggcaacaagc ccatcgtgca acggatgctc ggcagtgccg
      661 ccgacctttc acctgcgcaa tggatccggc tcaatcacat gtggatcggt tttttcctcg
      721 tcgttggcgc cgtcaacctg ttcgtcgcct acaacttcag cgaggaattc tgggtgaaat
      781 tcaagctgtt cggcatgctg ggccttacgg tcctgttcgt gattgttcag actgtctggc
      841 ttacggcggc aacgaagcgt aacgccatca ctgatgatga ggggaccgac tgatgtggta
      901 cgccgtgatc agcgaagata ttgaagacag cctgtcgcgc cgcagccagg cacggccagc
      961 ccatcttgca aggctgtcga cgctggttga tgaaggccga ttgcttgtgg ccggtcccca
     1021 ccctgcaatc gatgccgaga atcccggtcc cgctggattc agcggcagcc tggtaattgc
     1081 cgatttcgct tctctcgagg aagcggaagc ttgggcccgc gacgatcctt acgtcgaagc
     1141 tggagtgtac gcaaacgtcg ttgtcaagcc gttcaagtgg gtgctgccgt gaactcattg
     1201 cgcgaagcga gaatccggca gtgcctccgg gagagcctcg atccgcagga actattgatc
     1261 aaggaccaga gtcaactgca cattggccac gagggcgcca aggacggcaa aggccactat
     1321 gacctactgc tggtctcacg tgcatttgag ggggtgaacc gggtgcagcg gcatcgcatg
     1381 gtttatgatg ccctccgcga tttgctcgag tctgacatcc atgcgctccg aatcagcgct
     1441 tacgctccat ccgaacgata gcacagacgt tgctgctgtt cctctacact ttttacggac
     1501 attaactatg aagtcatcga acgccctgac acgtgtcagg atcgcctcag ttgccaccgt
     1561 gggtgcactg agccttgcca ctgcgacgtt agtgaacgcc gaggctgtca aaacggttaa
     1621 tggcacagag atcgacagtg cggtcctcga cgtctacatg gaaagtcggg tcaacaagcc
     1681 gatcgagcaa gttactgctg aagagcgtga ggcgcttatt cgcgagctaa cggacatcta
     1741 cctgctgtcc acgcaggact cggcgacgca cctggaagac gacccgcagg ttgcagccca
     1801 gatcgaactc cagaagcggg gtgtgctggc ccaggcggtt gccagtcagt ttttcgctga
     1861 aaacaaagtg actgaagaag aggtactggc cgagtattca gagcaagtga agttagctcc
     1921 accgcttcag tacaaagccc gtcacatcct cgtgccgaca caaggagctg cgaaagatgt
     1981 catctccgaa ctcgatgctg gtgccgactt ccaggaaatc gcgaaggaaa aatcgaccgg
     2041 accgtcggca cctagcggtg gcgatctcgg ctggtttgca ccaaaccaga tggttgcgga
     2101 attctctgat tcagtagcag gccttgagga cgggaagtac acgaaagaac ccgtacaaac
     2161 agagtttggc tggcacgtta tcttgcgtga ggagtcccgc caatccgagc cacctccgct
     2221 ggacagcgtt cgcgacgcac ttacccagaa tgtgcagcag aaaaaattcc aggcacacct
     2281 cgatgatctg cgagctaacg ctgagaagta ggttcgtttt acttaatctc gatatacgat
     2341 aaagaaaggg cgccccttgg gcgcccttca atgtggtgga attgttttgt gagtccgttc
     2401 acagcaaaag tgaaccggcc tctctatagt cacgtccgag tcgcttctaa tcgctcaggg
     2461 gcggctcctc caaccctcgc atctacttta tgttgatacg agatctaagt cggcagggac
     2521 gccgactttt ttttgtctgg cgtttgcctg gcttcgctcc ttccgatcaa ggctgcgcaa
     2581 ccagtaaccc caattgctgg tatacccttc cggttacagc aacctcatct ccgatgcttg
     2641 gtgcatgtcc ggttgaaagc aacgcgttgt aatcgaccgt aacgccactg acgactgccc
     2701 ggccagtcga gtagttgacc tcatcaacta ctccgcgcag gtaagacaag tcaccaccag
     2761 caaccggcac tacagatgcg ttgacaatgc ttcctgtctc tctgtcgatg ctgccgtaga
     2821 cggcgacggg ggtgccaaga gcaattccgt gccaattcga atcagagccg agtacagttt
     2881 gtcc
//
LOCUS       DASUOM010000645         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1425736,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000645 DASUOM010000000
VERSION     DASUOM010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425736"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFY27_13550"
     CDS             <1..725
                     /locus_tag="VFY27_13550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5766585.1"
                     /translation="FASEVIRNEVQALREAGIPVVASMSSVAASGGYWISMAADQIYA
                     SPSTITGSIGIFGMFPTFQRSLDSLGIHTDGVGSSKWAGELRLDRAMSDDAKALVQVM
                     IEDGYDDFISKVATHRNMEQAEVDRVAQGQVWTGTDALDHGLIDRLGGLEEAIAAAAE
                     LADLDAEAYGLKYYEKVLTPAEQLTLQFLSSAARIGLDLKGLVNRPSSMERVAGVVAE
                     ALSPMLRFNDPKGMYAHCFCVF"
     gene            complement(770..1795)
                     /gene="tdh"
                     /locus_tag="VFY27_13555"
     CDS             complement(770..1795)
                     /gene="tdh"
                     /locus_tag="VFY27_13555"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX5766586.1"
                     /translation="MRALVKAKAERGIWLEDVPLPVPGHNDVLIKVRRAAICGTDIHI
                     YKWDDWAQKTIPVPLTIGHEFSGEIVECGSEVRGLATGDRVSAEGHITCGVCRNCRAG
                     RRHLCRSAVGIGVSRAGAFAEYISVPAFNVFKLPDSITDEMAAILDPLGNATHTALSF
                     DLVGEDVLITGAGPIGIMAVAIARHAAARHIVVTDVNEYRLGLAKRMGASVALNVSGG
                     SLEGTMRELGMEEGFDVGLEMSGNPAAFRDLLRTMHHGGKVAILGIPPAEMAIDWNEV
                     IFKGLTLKGIYGREMFETWYKMASMLQSGLDIQPIITHRFGNEDYEQAFELMASGQSG
                     KVILRWN"
     gene            complement(1795..2991)
                     /gene="kbl"
                     /locus_tag="VFY27_13560"
     CDS             complement(1795..2991)
                     /gene="kbl"
                     /locus_tag="VFY27_13560"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX5766587.1"
                     /translation="MPDQFLDSLATRTAALKEQGLYKQERLIAGPQQAEIKVRKNGGS
                     QTVLNLCANNYLGLANHPRVISAAHKALDDFGYGMASVRFICGTQTIHKQLEERITRY
                     LGTEDTILYPSCFDANGGLFETLLEDKDAVISDELNHASIIDGIRLSKAQRFRFRNND
                     MANLEENLKQAANCRTRLIVTDGVFSMDGTIANLEEICKLASRYDALVMIDDCHATGF
                     LGDTGRGTHEYRGVMGKIDIITSTLGKALGGASGGFTSGRKEIVAWLRQRSRPYLFSN
                     SVAPAIAATSIAVLDLLESDNGLRRKLMENASWFRNSMEALGFELRPGQHPIIPVMLG
                     DAKLATTMADRLLDHGIYAIGFAYPVVPQGQARIRTQMSAALTRDHLERAVAAFQAVG
                     RELGVI"
     gene            3101..3523
                     /locus_tag="VFY27_13565"
     CDS             3101..3523
                     /locus_tag="VFY27_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX5766588.1"
                     /translation="MRSTLNTHKDKSRATGARLDRWLVQTRFFKTRALAARAVAGGHV
                     KRGWERARPGDKVSVGDRLEIVRGHERFEVEVMALPARRGPATEARACYYESEHSIAR
                     REAATARLKQDRLAMPRTKGRPDKHTRRLLRERNRSGD"
BASE COUNT          714 a         1128 c         1185 g          737 t
ORIGIN      
        1 cgtttgcatc ggaagttatt cgcaacgaag tgcaggccct tagagaagcc ggcattcctg
       61 ttgttgcctc gatgagcagc gtcgccgcct cgggcggtta ttggatatcg atggcagccg
      121 accagatcta cgcgagcccg tcgacgatta ccgggtcgat cggcatcttc gggatgttcc
      181 cgacgtttca gcgatcgctc gattccctcg gcattcacac cgatggtgtc ggatcgagca
      241 agtgggccgg tgaattgcgc ttggaccggg cgatgtcaga cgatgccaaa gccctcgtcc
      301 aggtgatgat cgaggatggg tacgacgact tcatcagcaa ggttgcgacg caccgtaaca
      361 tggagcaggc agaagtcgac cgcgtcgcgc agggccaggt gtggacgggc accgatgccc
      421 tcgatcatgg actcatcgac aggctcggcg gactcgagga agctatcgcg gccgccgcgg
      481 aactggcgga cctcgatgcc gaggcatacg gcctgaagta ctacgagaag gtgctgactc
      541 ccgcagaaca actgacgctg cagttcctgt cctcggcggc gcgcatcggc ctggacttga
      601 aagggctggt gaaccgtccc tcgtcaatgg agcgtgttgc gggcgtggtt gcggaggcgc
      661 tgtcgccaat gttgcgtttc aacgacccga aggggatgta cgcgcactgc ttttgcgtgt
      721 tttagggaca ggatttggct gggggacaga tttaactgaa tctgtcccct tagttccagc
      781 gcaggatcac cttgccggac tggcccgagg ccatcagctc gaatgcctgc tcgtagtcct
      841 cgttcccgaa gcgatgggtg atgatcggct gaatatcgag gccgctttgc agcatgctgg
      901 ccatcttgta ccaggtttcg aacatttccc ggccgtaaat gcctttaagg gtaaggccct
      961 tgaagataac ctcgttccag tcgatggcca tttccgcggg gggtatgccg agaatggcga
     1021 ccttgccgcc atgatgcatc gtgcgcaaca agtcgcggaa ggcggcggga ttgcctgaca
     1081 tttcaagccc gacatcgaag ccttcttcca tgccgagttc gcgcatcgtg ccctccagtg
     1141 atcccccgga aacgttgagg gccaccgagg ctcccatcct tttcgcgagg ccaagccgat
     1201 attcgttcac gtcggtgacc acgatgtggc gggcggcagc atgccgggca atcgccaccg
     1261 ccatgatgcc gatcggtcct gcgcccgtga tgagcacgtc ctcgccgacg aggtcgaagg
     1321 aaagggcggt atgcgttgcg ttgccgagcg ggtcgagtat cgccgccatc tcgtcggtga
     1381 tggagtccgg cagcttgaac acgttgaatg ccgggacaga gatatactcg gcgaatgccc
     1441 cggcccggct gacgccgatg ccgaccgcgc tcctgcacag gtgtcgccgg ccggcgcggc
     1501 agttgcggca gacgccgcag gtgatgtgcc cttcagccga taccctgtct cctgttgcga
     1561 gcccgcgcac ctcgctgccg cactcgacga tctccccgga aaactcgtgg ccgatcgtca
     1621 gcggcacggg gatcgtcttt tgggcccagt cgtcccattt gtaaatatgg atgtccgtgc
     1681 cgcatatggc ggcgcggcgg accttgatga ggacatcgtt gtggccgggc acgggcagag
     1741 gcacgtcttc gagccatatg cctctttccg cttttgcctt gaccaatgcg cgcatcaaat
     1801 gactccgagt tcgcgtccaa ccgcctggaa cgcggcgacc gcgcgctcta ggtgatcgcg
     1861 tgtcagtgcc gccgacatct gggtgcgaat tcgcgcctgc ccctggggaa ctaccgggta
     1921 ggcgaagccg atcgcgtaga tgccgtggtc gagcaggcgg tcggccatgg ttgtcgccag
     1981 tttcgcgtcg cccagcatca ccgggatgat ggggtgctgg cccggacgaa gctcgaatcc
     2041 cagcgcctcc atgctgttgc ggaaccagga ggcattctcc atgagcttcc tgcgcaaacc
     2101 gttgtcgctc tccagcaggt ccagcaccgc aattgacgtt gccgcgatgg cgggggcaac
     2161 agagttcgaa aacaggtagg gccgcgaacg ttgccgcagc cacgcgacga tttccttcct
     2221 gcccgaggtg aagccgccgg aagcaccgcc aagcgccttg ccaagcgtgc tggtgatgat
     2281 atcgatcttg cccattacgc cgcggtattc atgagtgccg cgtccggtat cgccgaggaa
     2341 accggtcgca tggcaatcgt cgatcattac cagcgcgtca tagcggctgg ccagcttgca
     2401 gatttcctcg aggttcgcga tcgtgccgtc catcgagaac acgccgtcgg tgacgatcag
     2461 ccgcgtacgg cagtttgccg cctgcttgag gttctcttca aggttcgcca tgtcgttgtt
     2521 gcggaagcgg aacctctgcg cctttgaaag gcggattccg tcgatgatgc tggcgtggtt
     2581 cagctcatcg ctgatcacag cgtccttgtc ttccagcagc gtctcgaaca gtcctccgtt
     2641 tgcatcgaaa caggaggggt acagtatcgt gtcctccgtg ccgagatagc gtgtgattcg
     2701 ttcttccagt tgtttgtgta tggtctgcgt gccgcaaatg aaccggaccg acgccatccc
     2761 ataaccgaaa tcatcaagag ctttgtgcgc agcactgatg acgcgcggat gattcgcaag
     2821 tccgaggtag ttgttggcac agagattgag aacggtctgg gagccgccgt tcttgcgtac
     2881 cttgatttcc gcttgttgcg gtccggcaat caggcgctcc tgcttgtaga gcccttgctc
     2941 cttgagagcc gcggtacgcg ttgccaggga atcgaggaac tggtcaggca tgggattgtc
     3001 ctcctggccg aaaggccgca gagtatatca ggaccgcgga caagccgaat atcgtccaca
     3061 atgtctattc cagctgcttc aactgccggc acagcaccag atgcggagca cattaaacac
     3121 tcacaaggat aaaagccgcg cgaccggcgc gcgactggac cgctggctcg tgcagacgcg
     3181 gttcttcaaa acgcgtgcgc tggctgcgcg agcggtcgcc ggcggtcacg tcaagcgggg
     3241 ctgggaacgc gccaggccgg gagacaaggt aagcgtcggg gaccggctgg aaattgtccg
     3301 cggccatgag cgcttcgagg tggaagtgat ggccctgccg gcacgaagag gtcccgcaac
     3361 cgaggcacgg gcctgttatt acgagtcgga gcattcgata gcgcgcagag aggctgcgac
     3421 agcacgcctg aaacaggacc ggctggcgat gccccgcacg aaaggacggc ccgacaagca
     3481 cacgcgccgc ctgctgcggg agcggaaccg ctcgggcgat tgagaagcac agctggaacc
     3541 taagtcgagc gctgcaggag cccgtctcgc gggagacaac gggatcccgg tagccaccgt
     3601 cgtggccacg cctttcgaac cgcaaaacct cctataattg ccgtcctttt ggccgccgca
     3661 cggtctcgac aacaggagcc gccgggcacg cccggaatgc ctcgatgaaa atttccgacc
     3721 agttcgcctg cagcctggaa cccctgaaac ccgtcaacga ccga
//
LOCUS       DASUOM010000646         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Woeseiaceae bacterium isolate SMAG_U1628 k141_1061573,
            whole genome shotgun sequence.
ACCESSION   DASUOM010000646 DASUOM010000000
VERSION     DASUOM010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961765
            Sequence Read Archive: SRR25158519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Woeseiaceae bacterium (soil metagenome)
  ORGANISM  Woeseiaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Woeseiales;
            Woeseiaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,715
            CDSs (total)                      :: 2,681
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 34
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Woeseiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061573"
                     /isolate="SMAG_U1628"
                     /isolation_source="soil"
                     /db_xref="taxon:2026806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.42166667 N 112.2986111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..388)
                     /locus_tag="VFY27_13570"
     CDS             complement(191..388)
                     /locus_tag="VFY27_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766589.1"
                     /translation="MSVKYYTQFRLTDEYYRGGNEFSGVVELSRPVDQNSDVTDIEAV
                     LAKNFDLRRSSVKLLSWSRLH"
     gene            785..2251
                     /locus_tag="VFY27_13575"
     CDS             785..2251
                     /locus_tag="VFY27_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5766590.1"
                     /translation="MRNLTIPARWVVLATLILLAGTVLAQSELRTTFFRDADTALADA
                     NEANAKLLAPTSYAAGMKDYQAAEAGLDRGRNVEYVRDKAADATKHFQEATRIARLGK
                     TVLAQVMKSRQDAANARAPDLSPDLWVRAQESFGDAIREVERGDLKDAKRKDIEATTL
                     YRDAELVAIKAQYLTETRRLLGEAERAKVERYAPITLGKAHQLLADAERELNENRYDA
                     DLPRSLAQQANYEARHAIYLSEIVRRVRDKNLTVEELVLQWESPLQSIADAANVVPQM
                     ANGYEQVEQELVTFVENLRAENQELQQESEANALRAADMAEEIRALDERLGGATAERT
                     ALVQRLEAQARVKEQFETIEQMFGREEARVFREGDNVILRLVGLSFDSGKSELKQENL
                     DLLAKVERAIDVFPRSELIIEGHTDSYGSDDMNQTLSQSRAEAVQQYMINAMRIPSYR
                     LIATGFGETNPIANNETASGRAQNRRIDVVIKPNLDPA"
BASE COUNT          540 a          715 c          753 g          382 t
ORIGIN      
        1 tttcgcacga ccggcggacg aaagctcggc agggccgcgc cggcgaccta gctcccaaaa
       61 aaaatcccgg caatgccggg aagtcagggg gaaaacgatc gccagaagct agggagcgtt
      121 gcaggagcct gcccggcggg cgagtgcgct gacgcctgat cgaacccgaa gagggctcct
      181 gcaatattcc ctaatgcagc cttgaccagc tcaggagctt gacggagctc cgccgcaggt
      241 caaaattctt cgccagcacg gcctcgatgt cggtaacgtc cgagttctgg tccaccggcc
      301 ggctgagttc caccacaccg ctgaattcgt taccgccgcg atagtactcg tcggtcaatc
      361 gaaactgcgt gtagtacttg acggacatga cccggaggct accggcggcg ccgaagagcg
      421 tctgtgagcc aaagcacaaa tcctcaggcc tgcagcctgc ggccgcggca gcagttccac
      481 aatggtgaaa accgtcacaa gcgcgctgcc ccggatgatc catgggtggc acaggcggag
      541 aaccggcgag aatcccgaag ttgactgcaa ctggcgtaaa atccgcggct tgaagcttaa
      601 gcggagcctc gttcggtgca tctttaaaga gatgcgttcc acagtttcgc ccgcgctgcg
      661 tatgtattat cgtcatgccg cctcggttca aggcttgggc gcaaataacg tatttccgga
      721 gccgaaagcg gcgtcgccgc cgccagcttg ccgtattact ttttttaatt taagcgaatt
      781 cagaatgaga aatttgacga taccggcccg atgggtcgtg ctggccacct tgatactgct
      841 cgcaggaaca gtgctcgcgc agagcgaact gcggacgacg ttttttcgtg atgccgacac
      901 agctctcgcg gacgcgaacg aggcgaatgc caagttgctc gcgcccacca gttacgccgc
      961 gggaatgaaa gactaccagg cagccgaagc gggcctcgac cgcggccgca acgtcgaata
     1021 cgtgcgggac aaggccgcag atgccacgaa acatttccag gaagcaacca ggatcgcgcg
     1081 actcggcaag acggttctcg cgcaggtcat gaagagccgg caggatgcag ccaacgcgcg
     1141 ggcacccgat ctgtcgccgg acctgtgggt gcgtgcccag gaatcattcg gggatgcaat
     1201 acgcgaagtc gagcgcggtg acctgaagga cgcgaagcgc aaggatatcg aagccaccac
     1261 gctctaccgc gatgccgagc tcgtcgctat caaggcccag tacctgacgg aaacgcgccg
     1321 gctgctgggc gaagccgagc gcgccaaggt cgagcgctac gcgccgatca cgctcggcaa
     1381 ggctcaccag ctgctggccg acgcggaacg tgaactcaac gaaaatcgct acgacgccga
     1441 cttgccacgg agcctcgcgc agcaggcaaa ctacgaggcg cggcatgcta tctacctgtc
     1501 cgaaatcgtt cgcagggtaa gggacaagaa cctgactgtc gaggaactgg tcctgcagtg
     1561 ggagtcgccg ctccagagca tcgccgacgc cgcgaacgtg gttccgcaaa tggcgaacgg
     1621 gtacgagcag gtggaacagg agctggtgac cttcgtcgag aacctgcggg ccgaaaacca
     1681 ggaactgcag caggaaagcg aggctaacgc gctgcgcgcc gccgacatgg cggaggaaat
     1741 acgggccctg gacgagcggc tcggcggcgc caccgcggag cgcaccgcgc tggtgcagag
     1801 gctggaggcg caggcgcggg tcaaggaaca attcgagacc atcgagcaga tgttcgggcg
     1861 cgaagaagcg agggtgttcc gcgaaggcga taacgtgatc ctgcggctgg tgggcttaag
     1921 cttcgacagc ggcaagtccg aactcaagca ggaaaacctc gacctgctgg caaaggtcga
     1981 acgggcgatc gacgtttttc cccgcagcga actcatcatc gagggacaca ccgactctta
     2041 cggcagcgat gacatgaacc agacgctgtc gcaaagccgg gccgaggcgg tgcagcagta
     2101 catgatcaat gcaatgcgga ttccgagtta caggctgatc gccacgggat ttggcgaaac
     2161 caacccgatc gcaaacaacg agactgcttc gggtcgcgca cagaatcggc gcatcgacgt
     2221 cgtcatcaag ccgaacctcg atcctgccta gcccggaacc agggagcctc tgcccgggta
     2281 ctggacggcg tgacggattc ttcggcactg ccggtcacgc tacgccacca gttccggatc
     2341 tccagtgcgg ttgcctcgtt gacgaggtag ccgtattcgg agtgcacgtt
//
